LOCUS       DAOSMY010000001        19427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_2812_length_19427_cov_4.157031, whole genome shotgun sequence.
ACCESSION   DAOSMY010000001 DAOSMY010000000
VERSION     DAOSMY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2812_length_19427_cov_4.157031"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLD76_00005"
     CDS             complement(<1..860)
                     /locus_tag="PLD76_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26422.1"
                     /translation="MSYKNLDQQNLSFLKELLSTNSPSGYEVNAAKVWQNYVNQFAEV
                     SGDVLNNSIGVLNPQAEFKIMIAGHYDEIGLQVMYISKEGYLYVRRLGGIDLLSLPGS
                     EVVVITKKGTISGVLGKIPIHVQSPTERTKAIELEDLWIDIGVDSEEEAANLVEVGDP
                     VAIKSNFHYLGQHKIVSKGMDDKIGAFIVAEVLRNLSTQQLKVGVYGVATSQEELGAR
                     GAITSTFKVNPHVGFCLDVGIATDVPGIEERKYGKMRLGGGASITHSADNNVVLVRLL
                     QQTAQQNNIQ"
     gene            complement(906..1364)
                     /locus_tag="PLD76_00010"
     CDS             complement(906..1364)
                     /locus_tag="PLD76_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU26423.1"
                     /translation="MPEITNERTMMLFKNEISAEFAQQLCEDADKVLEFVSQVKWKDG
                     FVCRNCNHTNYCEGKTPFSRRCTRCKKEESATAHTLFHNIKFPINKAFFIAYHVCMLG
                     DEFSSYTYSFQLGLNQMTCWKFRKRIQHCIAKVDKQDSNTIQGILLQTYE"
     gene            1471..2112
                     /locus_tag="PLD76_00015"
     CDS             1471..2112
                     /locus_tag="PLD76_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU26424.1"
                     /translation="MITDAINKRYSSLSETTCCLSCGGAINYANPLVGEVCVDLGSGR
                     GNDVVRLAETVGNNRYVYGIDISEGMIAKAQSLIEKFDIKNAKIIQSNLENIPLQDNS
                     VDVVISNCTINHASNKQAVWSEIFRILKPAGRFVVSDIYATHEIAAEYKNDPQAVAEC
                     WAGASTRATYLYTLEETGFESINILEESLPYAKGKAEVASFTVTGKKPIDKIR"
     gene            2358..3680
                     /locus_tag="PLD76_00020"
     CDS             2358..3680
                     /locus_tag="PLD76_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU26425.1"
                     /translation="MKKSKHTILVPITDSSDYFTVNPLHKSADILSENEAKCLKNNID
                     VEGNFTSKKYIIDEETEKRAFKLAYLDFIDKREDEETQLFFVPNYSCNFACSYCYQEG
                     YNPIQQKLTTEIIDAFFAYVATQLAQKKKYITIFGGEPLLPNEQQRELIAYFIKKATT
                     AQLDIAIVTNGYTLLSYIDILKKARIREIQVTLDGTEQVHNNRRYLKGKQSTFNQIVE
                     GIDACIEAEMPINLRMVVDKENIDNLVEFSQFAIDKGWTKSPYFKTQIGRNYELHFCN
                     STPDKLFDRATLYKKIYELLKEHPHIAEFYKPTFSISKYIAENEELPTPLFDSCPACK
                     TEWAFDFTGTIYSCTATVGKKGEELGTFYPHITHNESLIAEWQNRDITTIEACKNCEV
                     ALACGGGCASVAKNKHGHVNSPDCRPIKELLSLGAAYYFSNTPIDTKN"
     gene            3697..4971
                     /locus_tag="PLD76_00025"
     CDS             3697..4971
                     /locus_tag="PLD76_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU26426.1"
                     /translation="MKEITANEFDQEVLKGEKVVVDFYSTECPPCEALFPKYEAVAEL
                     YGHKIKFLKIYRQGNRELATQLGVSSSPTLLFFENGKQILDTISGGIKKSEITARLNK
                     MLTENEIAEIQKKQQRSVSEYDVAILGGGPAGLTAGIYLGQAKVKTVLIDPALPGGYM
                     GITHQVSNYPGFVEAQNGYMLAHYMSEQAKQTGIDFKVAVDITSVDLVKKEIVIDELE
                     TIKAKKIIIATGTSPNTMNAPGEKEYKGKGISYCATCDAKYFVDKEVTVIGGGNSAVE
                     EALFISKFASKITMIHQFDALTANKTAIEQLQANEKITVKYEHEPRKFEKRGEKMVTT
                     IENLRTKEIAELASDGVFVFIGFKANLSFLSTNAIPALDNWGYIQTDEDRKTSISDVY
                     AIGDIVSKKYRQITTAVADGTIAAITISKELN"
     gene            5172..6491
                     /locus_tag="PLD76_00030"
     CDS             5172..6491
                     /locus_tag="PLD76_00030"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU26427.1"
                     /translation="MKVLKFGGTSVGSAQRMQHVAKLICDGSQKIVVLSAMSGTTNTL
                     VEISDYLYKKNQGSANEVVNTLEKKYLAVINELYTTTEYKQKATEAIKQIFSYLRSFS
                     KSPVFSLFDEKEILAQGEMMSTNMMHFYLCEQGIKSTLLPALEFMRTDKNNEPDAAFI
                     KEHIAKQLSQYTDTEIFVTQGFICRNAYGEVDNLQRGGSDYSASLIGAAILADEIQIW
                     TDIDGMHNNDPRFVENTKPVAKLHFEEAAELAYFGAKILHPTCILPAKLENIPVRLLN
                     TMDPKAAGTLISTENTKGKIEAVAAKDNITAIKIKSGRMLLAHGFMRKVFEIFESYQT
                     PIDMITTSEVGVSVSIDNTKNLDPIVDELKKFGTVSVDKNMTIICVVGDLPSSNIGFQ
                     AKVVSALENIPIRMISYGGSDHNISFLIDAQDKIKALKALSNKLFNN"
     gene            6514..7674
                     /gene="lysA"
                     /locus_tag="PLD76_00035"
     CDS             6514..7674
                     /gene="lysA"
                     /locus_tag="PLD76_00035"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU26428.1"
                     /translation="MKKGLFPTNKFINLTTPFYYYDTVLLKATLESLKQETSKHNIEA
                     HYAVKANANDRILNIIAKEGFGADCVSGNEIKAALNAGFPASKIVFAGVGKDDWEINY
                     ALENNIFCFNCESIPEIEIINELAHKQQKIASIALRINPNVKADTHHYITTGVAENKF
                     GINLSDLEKVIDVVKKQANIKLIGLHFHIGSQILEMNNFKSLCTRINEIQQWMLDQKI
                     VIKNINVGGGLGINYQQPNENPIPNFKDYINVITQHLTLQPHQQLHIEPGRSIVAQCG
                     SLIAKVLYIKEGTHTKFAILNAGMTELIRPALYQAYHLIENITSEEEMEKYDIVGPIC
                     ESSDVFAKGMEINRAKRGDLVALRSAGAYGEVMASRYNLRPYPTVYLTEDFQ"
     gene            7689..8858
                     /locus_tag="PLD76_00040"
     CDS             7689..8858
                     /locus_tag="PLD76_00040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU26429.1"
                     /translation="MYSLFNVSFSLFQLIVISCLFIAFVVQLIYYTCCYSKLLTYWNS
                     IKKGKITFNTTLPPVSVIICAKNEADNLQKFLPSILEQDYPSFEVIVVNDGSTDDSED
                     ILMVLKHTYPHLRTTFVPQAAKYIDSKKIALVLGIKAAKNEILLFTDADCHTISKDWI
                     EKIVRNFDDNTEIVLGYGAYRKTKGFVNHLIGYDTLFIAIQYLSFALAKKTYMGVGRN
                     MAYKKSLFYKNKGFASHLNIQSGDDDLFISEASSKTNTKIEIHPNSITVSEPNTSLKR
                     WFNQKQRHISTSKYYKWRSKFILGTEVFSRGLFYISTIALMLALPSHTTYIVVGSFIF
                     VRYIAQITIINYAATILGERKYISSIPLFDILLPLFNLYVWCRSLIHRKQHYKWK"
     gene            8943..9758
                     /gene="bamD"
                     /locus_tag="PLD76_00045"
     CDS             8943..9758
                     /gene="bamD"
                     /locus_tag="PLD76_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOU26430.1"
                     /translation="MHKFYILFISVMVLLLSSCSNYQKLLKNPDPAIKYEAALEYFAK
                     KDYMRSLTLLQDITPVYRGTEKAENILYLIASSNFHQRDYISARHYYSVYISSYPRGK
                     YIEEVKYMNALSYYKESPEPKLDQTASSKAIELFNDFLLEYPKTDKLVQIQLLVSELQ
                     EKLAERELISATLYYNIGNYRGNNYYRSAIVTAENALKDYPKSKFKEDFSILVVRSKY
                     QEAIQSVENKLYERSVVALDECIYFLQEYPTSNYIKEVQQLKLELEKIIIKFT"
     gene            9773..10099
                     /locus_tag="PLD76_00050"
     CDS             9773..10099
                     /locus_tag="PLD76_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOU26431.1"
                     /translation="MDYKKTNAPKSTISRDTTSLCENTGNIYETVKIIGKRANQISVE
                     IKSDLERKLQEFSSNNVDSLEEVFENREQIEISKYYEQLPKPSLLAIQEYLEDKTYYR
                     NPSKMK"
     gene            10193..11389
                     /gene="coaBC"
                     /locus_tag="PLD76_00055"
     CDS             10193..11389
                     /gene="coaBC"
                     /locus_tag="PLD76_00055"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOU26432.1"
                     /translation="MLEGKKILLGITGSIAAYKSAYLVRELVKKGAEVKIIMTATAKE
                     FITPLTLATLSKNPILVDFFDPTNGNWNSHVKLGLWADAFLIAPASANTIAKMAHGIA
                     DNLLLTSYLSCKSPVFIAPAMDLDMYAHPSTQENISLLMQRNNYIIEATSGELASGLK
                     GKGRMEEPHIIVDILTNFFSKKSILQGKTILITAGPTYEKIDPVRFIGNYSTGKMGYA
                     LAEECAHNGAKVILISGPVGISVQHPNIMKIHVESAAEMAVATKKYFPVSDAAILSAA
                     VADFTPTIKAAEKIKREKNNLTLELSPTEDIAVAIGKLKKQDQRIIGFALETTNEKQH
                     AFEKLQRKNFDCIVLNSLNDSHAGFGYDTNKISIIDKQGNCEEYDLKLKKEVAKDIVA
                     KLITLF"
     gene            11398..12291
                     /locus_tag="PLD76_00060"
     CDS             11398..12291
                     /locus_tag="PLD76_00060"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOU26433.1"
                     /translation="MRKIQVLFICFIFATITMTAQELVAKVTVNADKIQGVNTQLINN
                     LEKTIAEFFNAQNFTDLVFETNERIDCSFFITIKEINSTNEMKADIQVQARRVAFNSM
                     YYTPLFLFKDENFNFTFTENTILAITDNSFDSNLSAVLTYYAYVIIGLDADSYSKLGG
                     TPFYEKAERITNLAQSTSESGWKAFESSKNRYALITNLLDESLRSFRLFFYDYHRLAL
                     DEMHISVDKARAKIAEDMPTLRAAYKNRPSAIILQVFTEAKVEELLSIFEKGTPNEKK
                     IVYETLSAVSPSLNNKLEPLR"
     gene            12298..13962
                     /gene="recN"
                     /locus_tag="PLD76_00065"
     CDS             12298..13962
                     /gene="recN"
                     /locus_tag="PLD76_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU26434.1"
                     /translation="MLLSLHIENYALIKQLDLNFDSGFSVITGETGAGKSIMLGAMGL
                     LLGQRADLKTIKEGENKCIIEASFDIRHYNLKSFFLENDVDYEETTLIRREIVTTGKS
                     RMFVNDTPVTIQFLKEIGQRLIDIHSQHENLLLKNSLFQLDIIDTVATHKDDIAAYTE
                     LYLSYKNKLRTLASLKESAEKEKNNYDYILFQYEQLTSIQLQAGEKDHLEEELHMLSH
                     AEEIKSSLIQANQLLQNDEHGIVTTLKQTQQLIAQLLHFFPQCTDLNERLLSCYIELK
                     DMTAEISKMESSVEYNPQQLQFVTDRLDSIYTLCQKHKVTEIEDLLALQESYKNQLEK
                     ILSFDTEIATLEKECNIVYELLIQKATEISINRKTSAPIIEEYMVEQLSTLGMPYVQF
                     KIEFKQVSEPQLYGFDDVEFLFSSNKNTTAKPVSDIASGGEIARVMLCLKSLIAQSST
                     LPTIIFDEIDTGVSGEIADKMGNIIQKMSKQMQVICITHLPQIAAKGTQHYQVYKVDT
                     DTSETRVKPLSTTERKEEIAKMLSGSNITEAALKNAEELLKQSSNG"
     gene            complement(13936..14973)
                     /locus_tag="PLD76_00070"
     CDS             complement(13936..14973)
                     /locus_tag="PLD76_00070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU26435.1"
                     /translation="MNKIAIVILNWNGRQYLERFLPSVVMYSTYPHVEIVVIDNASTD
                     TSLEFLSTTYPTITRVVLDKNYGFTGGYNKGVQHIDADYAVFLNSDVEVTANWLDKPI
                     EHLHTHPNTVAVQPKILSFENRAMFEFAGASGGYIDFLGYPFCRGRIISEIEQDNGQY
                     NKPIPIFWASGACLFVRLQEFIQYGGFDDFFFAHMEEIDFCWRMKSKGRDVFCIPESV
                     VYHVGGGTLTKENPHKTYLNFRNNLLLIYKNMLPKQALLVFFIRFFLDYLAAIMMLLQ
                     GKPNDAKAVYRARKDFARHKKAFSAVRLLNMQENVVAQYSEMYTGSILVDFYIRRKRR
                     FSELSIARLFQ"
     gene            complement(14974..15876)
                     /locus_tag="PLD76_00075"
     CDS             complement(14974..15876)
                     /locus_tag="PLD76_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU26436.1"
                     /translation="MPQLAYFLLYVVVRLFALLPFFVLYLLSDILYLIVYKLIGYRKK
                     VVRKNLKHSFPSFSQQQLLKIEKEFYHHFCDLFVETIKLSSMSTETIKQRFSVTNMEV
                     VEKLFAQGKSCISLSGHYGNWEWSAAYGLYIKPPAKALFLYKQVKNQAFDKLIQTLRQ
                     RFSGECIEKDFALRKMWQYKKQGVATFTGFLADQTPTARNIHYWTQFMNQDTPVFDGA
                     ERIARSMSYALLYIDVRKIKRGFYELTLIPMSDDVSATTEFEMTEQYVRLLEQTIQRN
                     PSYYLWTHRRWKHKRTQPTTSNIE"
     gene            complement(15892..16215)
                     /locus_tag="PLD76_00080"
     CDS             complement(15892..16215)
                     /locus_tag="PLD76_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26437.1"
                     /translation="MAEEDLIEFDDEAAISFILNYLPEEVKTKFSDDDVQYLLDVIYE
                     FYDKNGLLHEDVVEEVDIDEEELSAYIVKCAKEDKLPQFTEENVQLLLDAEYEYSKSI
                     GLFKE"
     gene            16365..17183
                     /gene="panB"
                     /locus_tag="PLD76_00085"
     CDS             16365..17183
                     /gene="panB"
                     /locus_tag="PLD76_00085"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU26438.1"
                     /translation="MSVHNNDLRKVTTHRLTEMKSRGEKISMLTAYDYSMASIVDQAN
                     MDVILVGDSASNVICGNVTTLPITLNDMIFLATAVTKAVHRALVVVDMPFGSYQGNSK
                     EAVASAIRIMKETGADSLKIEGGQEILESLTRILSTGIPVMGHLGLMPQSINKYGTYS
                     VRAKEEWEANKLMEDAIALEKAGCFAIVLEKIPANLAEKVAKTLRIPVIGIGAGNKVD
                     GQVLVMHDMLGINKGFSPRFLRRYADLHTSISNALQQYISDVKNGDFPSENEQY"
     gene            complement(17219..18058)
                     /gene="nadC"
                     /locus_tag="PLD76_00090"
     CDS             complement(17219..18058)
                     /gene="nadC"
                     /locus_tag="PLD76_00090"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOU26439.1"
                     /translation="MINDIAYLLKEWFSEDIGDGDHSSFSCIPSNAMGTSRLLVKEDG
                     VIAGVEVAKQVFAHFDNTLQVTVFIPDGTKVKKGDIVFDVTGNVRSLLQTERLMLNLM
                     QRMSGIASVTRVYVDELQGLATKVLDTRKTTPGLRFLEKMAVRIGGGHNHRFGLYDMI
                     MLKDNHVDFAGGIRNAIVAAQTYCKEKQKDLKIEIEVRNFEELNEVLQVGGVHRIMLD
                     NFSVENTQKAVAIIQGKYEVESSGGITLGTLRQYALCGVDYISVGALTHSVKSLDLSF
                     KAV"
     gene            complement(18051..18464)
                     /locus_tag="PLD76_00095"
     CDS             complement(18051..18464)
                     /locus_tag="PLD76_00095"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOU26440.1"
                     /translation="MKKTKYIYVLLPLMFFFIGGGVSVAQQEMIDRISEGFVRGNVSF
                     VSPYFNDRMELSILGQHIGVYTKLQSQEIVQAFLTNNTPQSFNISHKGIKENSGFAIG
                     ILTTTNNKKYRVYILVRNTNEKSLIHQLRIDETND"
     gene            18528..19001
                     /gene="rlmH"
                     /locus_tag="PLD76_00100"
     CDS             18528..19001
                     /gene="rlmH"
                     /locus_tag="PLD76_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOU26441.1"
                     /translation="MRIVLLVVGKTTNTYLQNLITDYQNRLKHYVSFDLQVIPELKNA
                     KHLSIEMQKEKEGECILKQLEDANDIILLDEKGTQFSSVDFAQHIDKAQVNGKKKILF
                     VIGGPYGFSEQIYQKATQKISLSKMTFSHQMIRLIFVEQLYRAFTILNNEPYHHE"
BASE COUNT         6868 a         3494 c         3247 g         5818 t
ORIGIN      
        1 tactggatat tgttttgctg tgctgtttgt tgtaacaacc ttactaaaac cacattatta
       61 tctgcactat gggtaataga agctcctccg ccaagtctca ttttgccata ttttcgttct
      121 tctatacctg gcacatcggt agcaataccc acgtctaaac aaaaacctac gtgtggattt
      181 accttaaaag tacttgtaat agcacctctg gctcccaatt cttcttgtga ggtagctaca
      241 ccatatacgc ctactttaag ttgttgtgtt gataaattac gaagaacctc agctacaata
      301 aatgcaccaa ttttgtcatc catcccttta gatacaattt tgtgttgacc taaataatga
      361 aaattggatt taatagctac tggatcgcct acttcaacca aattagcagc ttcctcttcc
      421 gaatctacgc ctatgtctat ccataaatct tctaactcaa tggctttggt tcgctcagtt
      481 ggcgattgaa catgaatggg gattttaccc aacacgccac ttatagtacc ttttttggta
      541 atcactacca cttcactacc tggcaaactc aacaagtcga tgcctcccag cctacgcaca
      601 tataagtaac cttctttaga aatatacatc acctgcaaac ctatttcgtc ataatgacct
      661 gcaatcataa ttttaaattc cgcttgggga ttaagcaccc ctatagaatt gtttaacaca
      721 tcaccactta cttctgcaaa ttgatttaca tagttttgcc atacttttgc cgcgtttact
      781 tcgtaacctg aaggagaatt tgttgaaagt aactctttta aaaaagatag attttgctgg
      841 tctaaatttt tgtaactcat cgttttttat ttaattagta tactatttga ttatataaca
      901 cgccattatt catacgtttg taacaaaatc ccttgtatgg tgtttgagtc ctgtttatcc
      961 accttagcaa tacagtgctg aatccgtttt ctgaatttcc aacaggtcat ctgattgagt
     1021 cctaactgaa aagaatacgt ataggacgaa aactcatctc ctaacataca cacatgatag
     1081 gctataaaaa aagccttgtt aatcggaaat tttatgttat gaaacaaggt atgagccgtt
     1141 gccgattctt cttttttaca acgcgtacaa cgccttgaaa aaggagtttt tccttcgcaa
     1201 taattggtat ggttgcaatt gcggcacaca aaaccatctt tccattttac ttgcgacaca
     1261 aactccagca ctttatcagc atcttcgcat aattgctgtg caaactcagc agatatttcg
     1321 ttcttaaaaa gcatcattgt tctttcgttt gtaatttctg gcaaatatat acattttttt
     1381 agtgataata aaataattca tagtgatact attgtaatat aaaataattt ttgtattttt
     1441 gcacattatt tacaataaac aagtatcttt atgataacag atgcaatcaa caaacgatat
     1501 tcttcattaa gcgaaacgac gtgctgtttg tcttgcggag gtgctataaa ctatgccaat
     1561 ccattggttg gtgaagtgtg tgtagatttg ggcagtggca gaggaaatga tgttgtacgt
     1621 ttggcagaaa cggtaggcaa caataggtat gtatatggta tagatatatc cgaaggaatg
     1681 attgccaaag cacaatcgtt gattgaaaaa ttcgacataa aaaatgcaaa aatcattcag
     1741 agtaatttgg aaaatatccc actacaagat aacagcgtag atgtagttat ttctaattgc
     1801 accataaatc atgcctccaa taaacaagcg gtgtggtctg agatttttcg cattttaaaa
     1861 ccagctggtc gatttgtggt aagtgatatt tacgccactc acgaaattgc ggctgaatat
     1921 aaaaacgacc cacaagcagt agctgaatgt tgggcaggtg catctacacg tgctacttat
     1981 ttatatacac ttgaggaaac tggttttgaa agcatcaata tactcgaaga atctttgccc
     2041 tatgccaaag ggaaagctga agtagccagt tttacagtaa caggaaaaaa accaatagat
     2101 aaaatccgat aacaacggat ctttcacttt tacttaattt aatactattt ttttatgaaa
     2161 agtttagtga aaaaacgctg tgcatgtaac agcaactcat cgaaacaagc tcaatgttgt
     2221 gctaaagttt cgagcatagt ggctggttgt cacgactaag tgtactatgc agagtaaccg
     2281 tatcggggaa ttcgttcccc gatatatttt attcatagtt agatttttaa cacccctatt
     2341 ttaatccact tttttgcatg aaaaaatcca aacacaccat acttgttcca attactgact
     2401 caagtgatta ttttacagtc aatccgcttc acaaatcagc cgacatcctc tctgaaaatg
     2461 aagctaaatg cttaaaaaac aacatagatg tggaaggtaa ttttacaagc aagaagtaca
     2521 tcatagatga agaaacagaa aaacgagctt tcaaattagc gtacctcgat tttattgaca
     2581 aacgtgaaga tgaagaaact caattgtttt ttgtgcccaa ttattcgtgc aattttgctt
     2641 gttcgtattg ctatcaagaa ggatataatc ccattcagca aaaactaaca acggaaataa
     2701 tagatgcatt ttttgcgtat gttgccacac aattggcaca gaaaaaaaaa tacatcacga
     2761 tttttggagg cgaacccttg ttgccaaacg aacaacaaag ggagctcatt gcctatttta
     2821 taaaaaaagc taccacggct cagcttgata ttgccattgt aacaaatggc tacaccttgc
     2881 tttcgtatat agacatacta aaaaaagcac gtattcgcga aattcaagtt acgcttgacg
     2941 gaaccgagca ggtacacaac aacagaaggt atttaaaagg caaacaaagc actttcaatc
     3001 aaatagttga gggtatagat gcatgcattg aagctgaaat gcccataaac ttacgtatgg
     3061 tagttgataa agagaacata gataatttag tagagttttc tcaatttgct attgacaaag
     3121 ggtggacaaa atcgccctat tttaagactc agataggtag aaactacgaa ttacattttt
     3181 gtaacagtac tcctgacaaa ttatttgata gggctactct ttacaaaaaa atatatgaat
     3241 tgctcaaaga acatccacac attgctgaat tttataaacc gactttctct atatcaaaat
     3301 acattgctga aaacgaagaa ctcccaactc ccctatttga ttcgtgtcca gcttgtaaaa
     3361 ccgaatgggc atttgatttt acaggcacaa tttattcgtg tactgctact gtcggcaaaa
     3421 aaggagaaga gctaggcacc ttttacccac acatcactca taatgaatca cttattgccg
     3481 aatggcaaaa cagagacatc actaccattg aggcttgtaa aaattgtgag gtagccctgg
     3541 cttgtggcgg aggctgtgct tctgttgcaa aaaacaaaca tgggcatgta aatagccccg
     3601 actgtagacc cataaaagaa ctactgtcgt taggtgcagc atattatttc tcaaataccc
     3661 ccattgatac caaaaattaa taaaacacaa tagaatatga aagaaattac tgcaaatgaa
     3721 tttgaccaag aggtcttaaa aggagaaaaa gtcgtagttg acttttattc aacagaatgt
     3781 cctccttgcg aagctttatt tccaaaatac gaggctgtag cggaattgta cggacacaaa
     3841 attaaattct taaaaattta ccgacaagga aacagagagc tcgccactca actaggcgtt
     3901 tcctcgtctc ccacactttt attttttgaa aacggcaagc aaattcttga caccatcagc
     3961 ggtggaataa agaaatcgga aattacagct cgtttaaaca aaatgttaac tgagaatgaa
     4021 attgctgaga tacaaaaaaa acaacaacga agtgtaagtg aatacgatgt ggctattttg
     4081 ggtggtggtc ctgcgggctt aactgctggc atctatttag gacaagcaaa agtaaaaacc
     4141 gtgctcatcg acccagcttt gcctggagga tacatgggca tcacacacca agtatctaat
     4201 tatcccggct ttgtagaggc acaaaatggc tatatgttgg cacactatat gagcgaacaa
     4261 gcaaaacaaa cgggcatcga ttttaaagta gccgtagata taacatcagt tgatttggtg
     4321 aaaaaggaaa tcgtaattga tgaattagaa accatcaaag caaaaaaaat tatcattgct
     4381 actggcacca gccccaacac aatgaatgct ccaggcgaaa aagaatacaa aggaaaagga
     4441 atttcatatt gtgccacctg cgatgctaaa tactttgttg acaaagaagt aacagtaatt
     4501 ggcggaggaa actcagccgt agaggaagcg ctgtttatta gcaaatttgc tagcaaaatt
     4561 acgatgattc atcagtttga tgcacttaca gccaacaaaa cagctatcga acaactacaa
     4621 gctaatgaaa aaattacagt taagtacgaa cacgaacccc gtaaatttga aaaacgtggc
     4681 gaaaaaatgg taacaaccat cgagaactta cgcaccaaag aaatcgctga attagcatct
     4741 gatggcgttt ttgtatttat tggatttaaa gcaaatcttt cttttcttag caccaacgct
     4801 atcccagctt tagataattg gggatacatc caaacagatg aagaccgcaa aacttcaatc
     4861 tctgatgtgt atgctatcgg agacattgta agcaaaaaat accgccaaat taccactgct
     4921 gtggcagatg gaaccattgc cgctataaca atatccaagg agctcaacta acctaattcc
     4981 tctaacaata tcctattttt gtagcatttt tgctgtcaaa aataggctat tatggtttct
     5041 aagtcctcat cttgtaacta tctattcaat tttctatttt tttacattca aaaataagtg
     5101 ggtaattcag taaaatgatt acctttgtac tccaaaattt caagctaaat aaacattaac
     5161 gcttaatcag aatgaaagta ttaaaatttg gtggaacatc tgtaggatcc gcacagcgca
     5221 tgcaacatgt agcaaaatta atctgcgacg gaagtcaaaa aattgttgtt ttatcagcta
     5281 tgtcaggcac aacgaatacc ttagtagaaa tttcagatta tttatacaaa aaaaatcaag
     5341 gtagtgctaa tgaggtggta aatacactcg aaaaaaaata cctagcggta attaatgaac
     5401 tatacacaac aacagaatat aaacaaaaag caacagaagc tattaagcaa attttttcat
     5461 acttacgtag ttttagcaaa tcgcccgttt ttagtttatt tgacgaaaaa gagattttag
     5521 cacaaggtga aatgatgtca accaatatga tgcatttcta tttgtgtgag caaggaataa
     5581 aatcaactct actcccagct ctagaattta tgcgtacgga caaaaacaat gagcctgatg
     5641 cagcatttat caaagaacac atagccaaac aactaagcca atatactgat acagagattt
     5701 ttgtaactca aggatttatc tgtcgcaacg cctatgggga agtagacaac ctacaacgtg
     5761 gtggaagcga ctattctgcc tctcttattg gtgccgcaat tttagccgat gaaattcaga
     5821 tatggacaga tattgatggt atgcacaaca atgaccctcg ttttgtagag aatacaaaac
     5881 cggtggcaaa attgcatttt gaagaagcag cagaactcgc ctattttgga gcaaaaatac
     5941 ttcaccctac ttgcatttta ccagcaaaat tagaaaatat tccagtacga ttgctgaaca
     6001 caatggatcc aaaagcagcc ggaacactta tttcaacaga aaacacaaaa ggaaaaattg
     6061 aagctgtagc agcaaaagac aatatcaccg ctattaaaat taaatcaggg agaatgctgc
     6121 tcgcccatgg ctttatgcgt aaagtgtttg aaattttcga aagctaccaa acacccatag
     6181 acatgattac tacctcagaa gtaggtgtgt ctgtttctat tgacaatact aaaaatttag
     6241 atccaattgt agatgaactg aaaaagtttg ggacagtaag tgttgataaa aatatgacaa
     6301 ttatctgtgt agtaggtgat ctaccctcat caaatattgg tttccaagca aaagtagtat
     6361 ctgctttaga aaatattccg attagaatga tttcgtacgg aggaagtgat cacaatattt
     6421 cttttttaat tgatgcacaa gataaaataa aagcattgaa ggctctaagt aacaaattat
     6481 ttaacaatta atcatctagt tacatcgttt caaatgaaaa aaggtttatt cccaacaaat
     6541 aagtttatta atctgacaac tcctttttat tattacgata ctgtattatt aaaagcaaca
     6601 cttgagtcat taaaacaaga gaccagcaag cacaacatag aggctcacta tgcagttaaa
     6661 gcaaatgcca atgatcgaat attaaacatt attgccaaag aaggttttgg tgctgactgt
     6721 gtaagtggaa atgaaattaa agcagcatta aatgcaggat ttcctgcatc taaaattgta
     6781 tttgctggag ttggtaaaga tgattgggaa ataaactatg cactagaaaa taatattttt
     6841 tgtttcaact gtgaatctat tccagaaatt gaaattatca atgagttggc tcacaaacaa
     6901 caaaaaattg catcaatagc cttacgcatt aatccaaatg taaaagcgga tacacaccat
     6961 tacattacaa ccggtgtggc agaaaataag tttggcatca atttatctga tttagaaaaa
     7021 gtaattgatg tggttaaaaa acaagcaaat atcaaattga ttggattgca ttttcatatt
     7081 gggtcgcaaa ttttggaaat gaataacttt aaaagtttat gcacccgtat aaatgagatt
     7141 caacaatgga tgcttgatca aaaaattgtt attaaaaata taaatgtagg tggaggacta
     7201 ggcatcaact atcaacaacc aaatgaaaat cccattccaa atttcaaaga ttatataaac
     7261 gtaataaccc aacatctcac attacaacct catcaacagt tacatatcga accaggacgt
     7321 tcaattgtag cacaatgtgg ttcgttaata gccaaagtac tatatattaa agaaggtact
     7381 catacaaaat ttgccattct caacgcagga atgactgaac tcattcgtcc tgccttgtat
     7441 caagcttatc acttaattga aaatattact tcggaagaag aaatggagaa atatgatatc
     7501 gttggtccaa tttgtgaatc atcggatgta tttgctaaag ggatggagat aaatcgagca
     7561 aaaagaggag atttagtagc tttacgttca gcaggtgcct atggagaagt aatggcatct
     7621 cgctacaatt tgcgaccata tccgactgta tatttaaccg aagattttca ataatagcag
     7681 tatgcagtat gtattcatta tttaacgtat cattttcgct atttcaactt attgtaatta
     7741 gttgtttatt cattgcattt gtagtgcaac taatttacta tacctgttgc tatagcaaac
     7801 tacttacata ctggaacagc ataaaaaagg gaaagataac tttcaatact acacttcctc
     7861 cagtaagcgt gataatttgt gctaaaaatg aagcggacaa cctacaaaaa tttctccctt
     7921 ctattctaga acaagactat ccgtcatttg aagtaatcgt ggtaaacgat ggatctactg
     7981 acgatagcga agatatatta atggtgttaa agcatacata cccacattta cgcactacct
     8041 ttgtgcccca agcagcaaaa tatattgatt ctaaaaaaat agcactagta ttgggcatca
     8101 aagccgctaa aaatgaaatt ttattattta cagatgctga ttgtcatact attagtaaag
     8161 attggattga aaaaatagta cgcaactttg atgataatac agaaattgta ctcggatacg
     8221 gagcctatcg taaaacaaaa ggatttgtaa atcacctcat aggatacgac acattattta
     8281 tagcaataca atatttaagt tttgctctgg caaaaaaaac ctatatggga gttggtagaa
     8341 atatggcata taaaaaatcc ttattttata aaaacaaagg gtttgcatct catctcaata
     8401 ttcaatctgg cgatgatgat ttatttatta gtgaagcttc ttccaagaca aatacgaaaa
     8461 ttgagataca tcccaacagt attaccgtat ctgaaccaaa tacatcttta aaaaggtggt
     8521 ttaaccaaaa acaacgacat atatccacat caaaatacta caaatggagg agtaaattca
     8581 tcttaggaac agaagttttt tcacgtggac ttttttatat cagcacaata gccctgatgc
     8641 ttgcattacc ctctcataca acttatattg tagttgggtc gttcatattc gtacgctata
     8701 tagcacaaat aacaatcatt aactatgctg caactatttt gggagaaaga aaatacatca
     8761 gtagtatccc attatttgat atattactcc cattatttaa cctatacgta tggtgcaggt
     8821 cgttaataca tagaaaacaa cattataaat ggaaataata gaattcttgt taaaaaaacg
     8881 ttttttattt tctgcattca gtacaaatat gtaagtttgc agattattca tcaaaaagac
     8941 ttatgcataa attttatatc ctttttataa gtgtaatggt gctgctattg tcatcatgca
     9001 gtaattacca aaaattactc aaaaacccag atccagctat taaatatgag gcagccttag
     9061 agtattttgc aaagaaggat tatatgcgtt ctttaaccct attacaagat ataactccag
     9121 tatatagagg cacagaaaaa gctgaaaata tcttgtattt aattgcatct agtaattttc
     9181 atcaacgaga ttacatttct gcacgacact attattctgt atatatttcg tcatatccta
     9241 gaggaaaata tatagaagaa gttaagtata tgaatgcatt gagttactat aaagaatctc
     9301 cagaacctaa attagatcaa actgcgagta gtaaagccat tgaattgttc aacgattttt
     9361 tactagaata cccgaaaacg gataagttag tacagattca acttttagtt agtgaacttc
     9421 aagaaaaatt agctgaaaga gaacttatta gtgccacctt gtattataat ataggtaatt
     9481 atcgtggcaa taactactat cgttcagcca tagttaccgc agaaaatgca ttgaaagatt
     9541 acccaaaatc aaaatttaag gaagattttt ctattttagt tgtacggtca aaataccaag
     9601 aagccattca aagtgtagaa aataagcttt acgaacgaag tgttgtggct ttagatgaat
     9661 gcatttattt tttacaagaa tatcccacaa gtaattatat aaaagaagtt cagcaactta
     9721 aattggaact tgaaaaaata ataataaaat ttacataatt tagctaaaac gtatggacta
     9781 caaaaaaacg aatgcaccca aaagtacgat ttctagagac accacaagct tgtgtgaaaa
     9841 cactggaaac atttatgaaa cagttaaaat tattggaaaa agagcaaatc aaataagcgt
     9901 tgagattaaa agtgatttgg aacgaaaatt acaagaattt tcttcaaata acgtagatag
     9961 cttagaagaa gtctttgaaa atagagaaca aattgaaatt tcaaaatatt acgaacaatt
    10021 accaaaacct tctttgcttg cgatacaaga atatttagaa gataaaacat actatcgcaa
    10081 tccgtcgaaa atgaaataac ctacgaattg ttatagtact aatttttgat aaaagaatgt
    10141 gggggtggtt gttttccgtt tcgatattct tttatctttt ttataaaaac ctatgttaga
    10201 aggaaagaaa attttattgg gaataacggg gagtatagct gcatataaat cagcatactt
    10261 ggtacgcgaa ttggtaaaaa aaggagccga agttaaaatt atcatgacgg ctactgccaa
    10321 agaatttatc acaccactta ccttagcaac tttatcaaaa aatcctattt tagttgactt
    10381 ctttgatcct acaaatggaa actggaatag tcatgtgaaa ttaggcttat gggcagatgc
    10441 ttttttaatt gctcccgctt cagcaaatac tattgcaaaa atggcacatg gaatagctga
    10501 caatctacta ctaacatcct atttatcgtg caaaagccca gtgtttatag ctccagctat
    10561 ggacttagac atgtatgcac atcctagtac acaagaaaac atctctctgt tgatgcaacg
    10621 caataactat attattgaag ccaccagtgg agaattagca agtggattga aaggaaaagg
    10681 aagaatggaa gaacctcata ttattgtgga tatactaact aacttttttt caaaaaaatc
    10741 tattttacaa ggtaaaacca tccttattac tgctggtcct acctatgaaa aaattgatcc
    10801 agtacggttt attgggaatt attctacagg aaaaatgggt tatgcactgg ctgaagaatg
    10861 tgcccataac ggagctaagg tcattctaat ttctggacca gtgggaataa gtgtacagca
    10921 tccaaacatt atgaaaatac atgttgaatc ggcagcagaa atggcagtag ccacaaaaaa
    10981 atatttccca gtttctgatg cagcaatttt gagcgctgcc gttgctgatt tcacaccaac
    11041 aataaaagct gctgaaaaaa taaaacgaga aaaaaataat cttacgctag aactttctcc
    11101 tactgaggat atagcagtag cgataggaaa attaaaaaag caagaccaac ggattatagg
    11161 gtttgcatta gaaactacaa atgaaaaaca gcatgcattt gaaaaactac aacgcaaaaa
    11221 ttttgattgt attgtgctca attcattaaa tgattctcat gcaggctttg gctacgacac
    11281 aaacaaaatt agcatcattg acaaacaagg aaactgcgaa gaatatgacc taaaattaaa
    11341 aaaagaagtt gcaaaagata ttgtagccaa attaattacc cttttttgac aagcgacatg
    11401 agaaaaatac aggtgctttt tatttgtttt atatttgcta ctatcacgat gactgctcaa
    11461 gaacttgtag caaaagttac tgttaatgca gataaaatac agggggtaaa tactcaactt
    11521 attaataacc tcgaaaaaac tattgctgaa tttttcaatg cacaaaattt cactgattta
    11581 gtatttgaaa caaatgagcg tattgactgc tcttttttta taacaatcaa agaaataaat
    11641 agcactaacg aaatgaaagc cgacatacaa gtgcaagctc gaagagttgc attcaatagt
    11701 atgtattata cccctctttt cctttttaaa gatgaaaatt ttaatttcac ttttacagaa
    11761 aacactattc ttgctattac agacaattct tttgattcga acctatctgc tgtgttaact
    11821 tactatgcct atgttatcat tggattagat gcagattctt atagcaaact aggcggaaca
    11881 cctttttacg aaaaagcaga acgaataaca aatcttgctc aaagcacttc tgaaagcgga
    11941 tggaaagcct ttgaaagctc aaaaaatagg tatgccctaa tcacaaacct actcgacgaa
    12001 agtttacgtt cttttcgctt atttttctat gactatcatc gtttagctct cgacgaaatg
    12061 catatttcgg tggataaagc tagagctaaa attgcagaag atatgcccac tttacgagct
    12121 gcatataaaa atagaccgtc agccattatt ttacaagtat ttacggaagc aaaagttgaa
    12181 gaattactgt ctatctttga aaaagggacg cccaatgaaa agaaaatagt ttatgaaaca
    12241 ctttcagctg tttctccatc actcaataat aaacttgaac cgctacgata aaatcctatg
    12301 ttattatctt tacacataga aaattatgct ctaatcaaac aattagactt aaactttgac
    12361 tcaggttttt ctgttattac aggtgaaaca ggagcaggga aatctattat gttaggagca
    12421 atgggattgt tgttgggtca acgagctgac ttaaaaacaa ttaaagaagg agaaaacaaa
    12481 tgtattattg aagccagttt tgatattcga cactataatt taaagtcctt cttccttgaa
    12541 aatgatgtag attatgaaga aacaaccctt atacgaaggg aaatcgtaac aacaggaaaa
    12601 tcacgcatgt ttgtcaatga tactcctgta actatacaat ttttaaaaga aattggacag
    12661 cgacttattg atatacactc gcaacatgaa aacctcttac ttaagaattc tctttttcaa
    12721 ttggatatca ttgatactgt ggctacacat aaagatgaca tagctgcata tacagaatta
    12781 tatctttcgt ataaaaataa acttcggact ctagcttcat taaaagaatc tgcagaaaaa
    12841 gaaaaaaaca attacgatta cattttattt caatacgaac aattaacaag tatacaacta
    12901 caggctggcg aaaaagacca tttagaagaa gaattacaca tgctatcgca tgccgaagaa
    12961 attaaatcct ctttgataca ggctaatcaa ttactgcaaa acgatgaaca tggcatagta
    13021 actacactta aacaaacgca acagcttatt gcacagctac tacatttttt tccgcaatgt
    13081 acggatttga atgaaagact tttatcgtgt tatattgaat taaaagatat gactgctgaa
    13141 ataagcaaaa tggaaagctc agtagaatac aatccccaac aacttcaatt tgtaactgat
    13201 agacttgaca gtatctatac cttgtgtcaa aaacataagg taaccgaaat tgaagatctg
    13261 ctagcattac aagaatcata caaaaatcaa cttgaaaaaa tactgtcctt tgatacagaa
    13321 atagccacat tagaaaaaga atgcaatata gtatacgaat tactcataca aaaagcgact
    13381 gaaatatcta ttaacagaaa aacttcagca ccaataattg aagaatacat ggtagaacaa
    13441 ctatctacct tagggatgcc gtatgtgcaa tttaaaattg agtttaaaca agtatcagaa
    13501 cctcaattat atggatttga cgatgtagaa tttttatttt catccaataa aaacacgaca
    13561 gcaaagcctg taagcgatat tgcttctgga ggggaaattg ctcgtgtaat gttatgtttg
    13621 aaaagcctta tagcacaaag ctcaacttta ccaactatta tttttgatga aattgatact
    13681 ggcgtatcgg gtgaaattgc agataaaatg gggaacataa tacaaaaaat gagtaaacaa
    13741 atgcaagtta tttgtatcac ccacttacca caaattgcag caaaaggaac acaacactat
    13801 caagtatata aagtagatac agacacctca gaaacaagag tgaaaccact ttctacaaca
    13861 gaaagaaaag aagaaattgc taaaatgctt agtggtagta atattacaga agctgcgtta
    13921 aaaaatgcag aagaattact gaaacaatcg agcaatggat aattcagaaa atcttctttt
    13981 acgtcgaata taaaaatcga caagaatact tcctgtatac atttctgaat actgagcaac
    14041 gacattttct tgcatattga gtaaccgaac tgcactaaaa gcttttttat gcctagcaaa
    14101 atcttttctc gccctatata ctgcctttgc atcattcggt tttccttgta agagcatcat
    14161 aatggctgct aaataatcta aaaagaaacg tataaaaaac accaatagag cctgtttagg
    14221 caacatattt ttatagatta acaataaatt atttctgaaa tttaaatatg ttttatgagg
    14281 attctctttg gttaaggttc cacctcctac atgataaact acgctctctg gaatacaaaa
    14341 aacatccctc ccttttgatt tcatacgcca acaaaaatct atttcttcca tgtgtgcaaa
    14401 gaaaaaatca tcaaaaccac catattgaat aaactcctgc aaacgcacaa acagacaagc
    14461 tcctgaagcc caaaaaatgg ggataggctt attatactgt ccgttatctt gctctatttc
    14521 actaataatt cttcctctac aaaagggata acctaaaaaa tcaatatagc ctccactcgc
    14581 tcctgcaaac tcaaacatag ctctattttc aaaactcaaa attttaggtt gaacggcaac
    14641 agtattagga tgggtatgta aatgctctat aggtttatct aaccaattag ctgttacttc
    14701 tacatctgaa ttgagaaaaa ccgcataatc ggcatcaata tgttgaacac ccttattata
    14761 tcccccagta aaaccataat ttttatccaa tactacacga gttattgttg gataggtagt
    14821 tgaaagaaac tctagcgatg tatcagtaga tgcattatca atcactacta tttctacatg
    14881 tggataggta gagtacatca caacactagg caagaatcgt tctaaatatt gtctcccatt
    14941 ccaatttaat ataactatgg caattttatt catttattct atattactag ttgttggttg
    15001 agttcgttta tgtttccaac gtcggtgtgt ccacaaataa taagatggat tacgttgaat
    15061 agtctgctca agtaaacgaa catattgctc tgtcatttca aattcagttg tagcagaaac
    15121 atcatcagac atcggaataa gagttagttc ataaaaccct ctttttattt ttcggacatc
    15181 aatatacaat aaagcatatg acatactacg tgctatacgt tcagctccat caaacacggg
    15241 tgtatcttga ttcataaatt gtgtccaata atgaatatta cgtgcagtag gtgtttggtc
    15301 tgccagaaaa ccagtaaatg tagcaacacc ttgtttctta tactgccaca tcttacgtaa
    15361 tgcaaaatcc ttttcaatac actcgccaga aaaacgctga cgtaatgttt gtattaattt
    15421 atcaaatgct tgatttttaa cttgcttata caaaaacaac gctttagctg gaggttttat
    15481 atataaacca taagctgccg accactccca attaccataa tgtcctgaca acgatataca
    15541 acttttacct tgagcaaaaa gtttttcaac tacctccata ttcgttacac taaaacgttg
    15601 cttgatagtt tcagtggaca tactactcag tttgatagtt tctacaaaca aatcacaaaa
    15661 atgatgataa aactctttct ctatcttcag taattgttgt tgcgaaaaag aaggaaaaga
    15721 atgtttaaga ttcttgcgta ctactttttt tctatagcca attagcttat atacaattaa
    15781 ataaagtata tctgacagta gatacaacac aaaaaatggc agtaaagcaa aaagccttac
    15841 tacaacatac aacaaaaaat aagctaattg tggcattagc aaaaatgtat cttattcttt
    15901 aaacaaaccg attgatttag aatactcgta ttcagcatct aatagcaact gaacattttc
    15961 ttctgtaaat tgtggcaatt tatcctcttt ggcacattta acaatatacg ccgacaattc
    16021 ttcttcgtcg atatccactt cctctaccac gtcttcgtgc aagagtccat ttttatcgta
    16081 aaattcatag ataacatcca ataaatattg tacatcatca tccgaaaact ttgttttcac
    16141 ctcttcgggt aaataattaa gaataaacga gatagctgct tcgtcgtcaa attctattaa
    16201 atcctcttct gccataacta gaaatttaac tggttaaata attttaggta aagatacaaa
    16261 tgaaattgtt attagcaatg aattttaatt attttttaat atcggcaatt tgaataagat
    16321 tttattcgta aatttgcaat cttgtttaat taaaaagaac aactatgtca gtacataata
    16381 atgatttgag aaaagtaacg acacatcgat tgaccgaaat gaaatcgcgt ggagaaaaga
    16441 tttccatgct tacagcatac gattattcga tggcgagtat tgtcgatcag gctaatatgg
    16501 atgttattct tgttggcgac tcagcatcta atgttatttg tggcaatgta actaccctgc
    16561 ccattacact taatgatatg atttttttag caactgctgt tacaaaggct gtacatagag
    16621 ctttggttgt tgtggatatg ccatttggtt cctaccaagg caattctaaa gaggctgttg
    16681 cttccgctat ccgtattatg aaagagactg gagcagatag cttaaaaatt gaaggtggtc
    16741 aagagattct tgaatcatta actcgcattt tatccactgg tatacccgtt atgggtcact
    16801 tgggtttaat gcctcaatca ataaataaat acggcacata ttcggtacgt gccaaagaag
    16861 aatgggaagc caataagctc atggaagacg ccattgccct tgaaaaagcg ggttgttttg
    16921 ccattgtgtt ggaaaaaatt cctgccaacc tagccgaaaa agtagctaaa acgcttcgca
    16981 tacccgtaat tggtattgga gcaggcaata aagtagacgg acaagtgttg gtaatgcacg
    17041 atatgttggg tattaacaaa ggattttctc ctcgcttttt acgcaggtac gctgatttgc
    17101 acacttccat cagcaatgca ttgcaacaat atataagtga tgttaagaac ggtgattttc
    17161 cgagcgaaaa cgaacaatat taacgaaaaa aagaagagaa atttctcttc tttttctttt
    17221 atactgcttt aaaacttaaa tcgagggatt tgactgaatg cgtcaatgct ccaacagata
    17281 tataatctac cccacacaac gcatactggc gtaaggtgcc cagtgttatg ccacccgagg
    17341 actctacctc gtacttgcct tgaataatgg caacggcttt ttgcgtattt tcaacagaaa
    17401 aattatccaa cataatacga tgtacaccac ccacttgaag cacttcgttt aactcttcaa
    17461 aatttctaac ttcaatctct attttcaagt ctttctgctt ttctttgcaa taggtttgtg
    17521 cagcaacaat tgcattacga atgccacctg caaaatccac atgattatct tttaacataa
    17581 tcatatcata caatccaaaa cgatggttat gaccgccacc aatgcgtaca gccatttttt
    17641 ctagaaaacg tagacctggt gttgttttgc gtgtatcaag cacttttgta gcaagacctt
    17701 gcaattcatc tacatacacc cgtgtaacgg aggcaatacc actcatacgt tgcattagat
    17761 tcaacatcaa acgttctgtt tgcaacaaag agcgtacatt acccgttacg tcaaacacga
    17821 tatctccttt ttttactttt gtgccatcgg ggataaaaac tgttacttgc aatgtgttat
    17881 caaaatgggc aaacacctgc ttagcaacct ccactccagc aataacgcca tcctctttaa
    17941 ctaataatct tgaagtgccc atggcgttgg atgggataca tgaaaaagac gaatggtctc
    18001 cgtcgcctat atcctccgaa aaccactctt ttagtaaata agcaatatca ttaatcattt
    18061 gtttcgtcaa ttcttagctg atgaataagg gatttttcat tcgtatttcg caccaaaata
    18121 tacaccctat actttttgtt gtttgtagtt gtaaggatac caatagcaaa gcctgaattt
    18181 tcttttatgc ctttgtgcga tatattaaac gattgtggtg tattattcgt caaaaaagct
    18241 tgtacaattt cttgggattg caattttgta tacactccaa tatgttgtcc caatattgac
    18301 aattccattc tatcattaaa ataaggagaa acaaacgaaa catttccacg tacaaaaccc
    18361 tccgaaatac ggtcaatcat ttcttgttgt gccaccgaaa cacctccccc aatgaaaaaa
    18421 aacatcagtg gcaataacac atatatgtat ttagttttct tcatattggg aagttatgca
    18481 gttttgttct acttttgcag aatacaatgc aaacataatc aaaaaaaatg agaattgtac
    18541 tacttgtggt cggaaaaacg acaaatactt atttacaaaa cttgattact gattaccaaa
    18601 atcgcctgaa acactatgta tcatttgatt tacaggtgat tccagaactt aaaaatgcga
    18661 agcatctatc tatagaaatg caaaaagaga aagaaggtga atgtatccta aaacaactgg
    18721 aagatgcaaa cgatattatc ctgttagatg aaaaaggaac tcagtttagc tccgtagatt
    18781 ttgctcagca cattgacaag gctcaagtaa acggaaagaa aaaaatacta tttgtgattg
    18841 gtggtccgta tggattttcc gaacaaatat atcaaaaagc tacgcaaaaa atatcgctat
    18901 caaaaatgac tttttcgcat caaatgattc gccttatttt tgtggaacaa ctatacaggg
    18961 cttttactat tctcaataat gaaccttatc atcatgaatg agggaataat tagtggatag
    19021 ttgacagtgg aaagtggata gtgattggtt ttgggggatt tattatacgg aggtcgtttg
    19081 tttttcgtgt gagagttatt acacagagat acacagagtc tccacagagt ggcacggagg
    19141 atttgatgcc ccgtaggggc tacatcataa tagaaaatac ataccgattt gtttgttagc
    19201 tccagcggag cgaaatagat atctttgtgc tcttcgtgtg agagttttta cacagagaga
    19261 cacggaggtt gcacagagtg gcacggagtt gtatatattt ttctcagtgt atcttcgtgt
    19321 ttacttcgtg ttcttcgtgt aagaaatagt acacagagag acacggaggt ttcacagagt
    19381 ggcacggagt tgtatatatt tttctcagtg tatcttcgtg tttactt
//
LOCUS       DAOSMY010000002        17932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_3160_length_17932_cov_3.465570, whole genome shotgun sequence.
ACCESSION   DAOSMY010000002 DAOSMY010000000
VERSION     DAOSMY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17932
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3160_length_17932_cov_3.465570"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="PLD76_00105"
     CDS             <1..2031
                     /locus_tag="PLD76_00105"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOU26442.1"
                     /translation="NTATDWSKVLVTDEFDTDQILNNRFFGLVRIGSVRDVVIKHHDL
                     QLPVGITNSIVVSCDIGDDVAIHNVGYLSYYIIGDRCIISNVNELNTCNHAKFGNGIV
                     KEGEPEKIRVWLELMNETGCRGVAPFDGMIAADAYMWARYRDDEALMKALTQITQNSF
                     DTRRGYYGTIGDECVVKNSFVIKDVKVGTHCYIKGANKLKNLTINSSIEEPTQIGEGV
                     ELVNGIIGYGCHIFYGCKAVRFVMGNNSNLKYGARLINSFLGDNSTISCCEVLNNLIF
                     PAHEQHHNNSFLVAALVMGQSNLAAGATIGSNHNSRANDNEVQAGRGFWPGLCTSIKH
                     SCRFASFVMLSKADYPAEMYIPFPFSLLNNNVSKDQLEVTPAFWWMYNMYALERNTWK
                     FKGRDGRKRKVQHIELEALAPDSVEEIFDALHLLELWVGKAFYRKQQQNLSQLTDEAM
                     QQKGKELLFSTAEAVNALEVLGEGMEKTKRKVLIIKAYQSYHAYRDMVYYYAVNTIFN
                     WMQHDTSRTFADMVVALQGNRETKWANFGGQLMREQDVDALRQDIGNGKLTTWNQIHQ
                     RYDSLWEQYPLQKQQHAYASLCSVLGVSNLTQKQWLEVLDKSLVIQEFVRDQVYLSRK
                     KDFDNPYRRMTFRNEAEMTAAIGTIEDNGFVKEVRQMTVKFNDLVKEIKQRG"
     gene            2071..3084
                     /locus_tag="PLD76_00110"
     CDS             2071..3084
                     /locus_tag="PLD76_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26443.1"
                     /translation="MNLTDKKYANYALVQEMMETVKVVQKFNAQQTKNVASTIQSVGK
                     LFLTGEGSSRIFPAKNAIRKSLTKGLPVSIATDGSRQSAQYDLSKFAVFCASNSGRTK
                     EVVLLAKQLAAIGNVNRFALSANSGTLLEEVCTETFVLTCGWEQAVAATKSVVEQALF
                     YESILWHIEQTGTPNLADLAAKIEQALTFTIDSSIVQAAAKAPTIYFAGYNDGVAEEL
                     TLKTNEITRKKSDFLEGTYAVHGIEEVMEAQDVVFLIDPIEEEIQKFQEVLEQGVCLK
                     IIAIASRPTPFTTILVPDAGALSPYVYLCAGWNLLVEIGLALGIDLDKPERARKVGNE
                     LIV"
     gene            complement(3218..4177)
                     /locus_tag="PLD76_00115"
     CDS             complement(3218..4177)
                     /locus_tag="PLD76_00115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU26444.1"
                     /translation="MIQKIKALLREVISFIKSDFDAVSYLYTLTFCGICIYVNYTYNL
                     YDTFLVPSYSSGWSMLYFSIFYMAAYFMVSIPVLIIRKQTAILTQPLYYVKAIFFIVS
                     FAVVVGNFTYQDLKIESFTYLENHFLIRLIFFFKANITTLPLLIIAKYLWDRKEKGLY
                     GLTISNKHIAAYMSLFLFLLPFLVWISFTPDFMRAYPIYRYWWYEGIFHWENWQHTII
                     FESVYASFYVITELVMRGALIIGMASILGRHAILPMVAMYCVLHFGKPIGETISSIFG
                     GYILGVLAYQTRHIWGGVIIHIAIALSMEVMGFIHYYLGIHKS"
     gene            4273..5793
                     /gene="guaA"
                     /locus_tag="PLD76_00120"
     CDS             4273..5793
                     /gene="guaA"
                     /locus_tag="PLD76_00120"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU26445.1"
                     /translation="MAEKILILDFGSQTTQLIARRVRELNTYCEIVPYNKFPVGDTSI
                     KGVILSGSPFSVYDQAAFKTDLSLFRGQLPVLGICYGAQFIAQQSGGKVQPANSREYG
                     RAKFSVCQSNNPLLKGIQEKSQVWMSHGDTITDIPANFSIIASTHDVEIAAYAVQGEQ
                     TWGVQFHPEVFHTTDGSKLLDNFLNICGCKKDWSAASFIETTVAELKAQLGDDKVVLG
                     LSGGVDSSVAAVLLNKAIGKNLTCIFVDHGLLRKNEFETVLDDYAHLGLNVIGVNVKD
                     RFYAKLAGVTDPEQKRKIIGKEFIEVFDEEAHKIQDVKWLAQGTIYPDCIESLSITGT
                     VIKSHHNVGGLPEKMNLKLCEPLRLLFKDEVRKVGNELGMMSHLIKRQPFPGPGLAVR
                     ILGDITAEKVRILQEADDIFIKGLREWNLYDQVWQAGVILLPVQSVGVMGDERTYENA
                     VALRAVTSTDAMTADWAHLPYEFLGKMSNEIINKVKGVNRVVYDISSKPPATIEWE"
     gene            5883..6311
                     /locus_tag="PLD76_00125"
     CDS             5883..6311
                     /locus_tag="PLD76_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU26446.1"
                     /translation="MKSKLFIIGVLVNLFIGFAGIAYAQQSANNSNSTSSEVSAMAFE
                     KTVHDFGTFNEADGKVETVFVFKNTSKENLVVSRVQASCGCTTPDWSKEPIAPGKKGF
                     VKASYNPAGRPGNFNKSVTVTSNQGTQVLYIKGQVVPKAN"
     gene            6480..7532
                     /locus_tag="PLD76_00130"
     CDS             6480..7532
                     /locus_tag="PLD76_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU26447.1"
                     /translation="MKYFTLLCLTFILIYGKVYAISFDALTHDFGTIYEEKGTVSTTF
                     SFKNKSNKLLVIERIVPACGCSNTHISSRNLAAGESGNIVVEFDPSERPGNFDKSIYV
                     YSNDSTVRLSIQGKVIPSKQTIAKMYPYRFQFSRADKNTLSFGSILHTQQSTQYVNFI
                     NDSDKPVKLTFLQLPPFISTDSLFYNLLPGNEQIIPFHISVLGHNVWGEKNEKLVVVE
                     NGKPSNAIQINVTVNEDFSILSAKERSRMPKIKLSSNEIIFGKTKQPSIETKELTITN
                     IGHGQLLIRKIELTDPALRVSITKTKLNRNKRAQVKISLLSDKSLGEKEHIFNILTND
                     PIHPVQTIIVKSSIYK"
     gene            7537..8631
                     /gene="meaB"
                     /locus_tag="PLD76_00135"
     CDS             7537..8631
                     /gene="meaB"
                     /locus_tag="PLD76_00135"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU26448.1"
                     /translation="MNHIENNEEYGGLVVNKGIAQPPTVNPNLASSRKKRTIYTANDY
                     LDGIIKGNRTILSQAVTLIESTKPEHYAMAQELIEKCLPYAKKSVRIGITGVPGAGKS
                     TFIEALGMYVVNQHKKLAVLAIDPSSQRTKGSILGDKTRMEELSVHENAFIRPSPSAG
                     SLGGVARKTRETIVLCEAAGFDTIFVETVGVGQSEIAVHSMVDFFLLLQITGAGDELQ
                     GIKRGIMEMADGIVINKADGANVDKAEMAKTQYKTALSLFPMHESGWKPQVVTCSAFY
                     KTNIDAIWTMLMQYIQFTQQSGYFETKRHQQASYWMYESINQELRSSFYAHSEVNALL
                     KEMEVKVTNGEISSFIAAKRLLDVYDKIRK"
     gene            8650..9264
                     /locus_tag="PLD76_00140"
     CDS             8650..9264
                     /locus_tag="PLD76_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOU26449.1"
                     /translation="MKSFGNYIFILVMLVACSQYKSKSILQGTITDSRYKKVYLLQQI
                     TPTSWEMLDSTSIVDNHFMFADLPSEYNQLFVQLDNTIPFVVFVDKGTIELAIPAQNV
                     HETKVKGSSLHDEYMMLQDSIDFLLHQRLLFYKDAIVAQNDGRQTDATILQQKYIHSK
                     NNVFHFLQKQKGANPTSAPLLYIIKTYKPYFTDTEYRTLSASLE"
     gene            complement(9266..9745)
                     /locus_tag="PLD76_00145"
     CDS             complement(9266..9745)
                     /locus_tag="PLD76_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOU26450.1"
                     /translation="MAQYQLDSLDEKILQLISKNARTPFLEVARECNVSGAAIHQRVQ
                     KLISVGVIKGSEFIIDPVKIGYNTCAYMGIFLKDANFFKEVVDELLKIPEVVECHYIT
                     GKYAMFIKIYARNNSDFLSIIHDKLQSIKGIASTETLMSLGETFKRQITVEGVADKE"
     gene            9993..10694
                     /locus_tag="PLD76_00150"
     CDS             9993..10694
                     /locus_tag="PLD76_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOU26451.1"
                     /translation="MATKRLKEIKPIEETADIWTLLSSDQAHYLQTNLQVISAKKNEV
                     IYSDGEIPSHLFCLVSGKVKVYKQGIGGRNQIVRVIKPNELFGYRAMFALEAYVTCAS
                     AIEPCVIYAIPEDVIVSIIKNNAQLAFAFIQFLAVDLGISDARTVTLTQKHIRGRLAE
                     SLLFLRDNYGVESDGITLSIYLSREDLASLSNMTTSNAIRTLSLFSNEGMIVVEGRKI
                     KIVDVESLVKISKFG"
     gene            complement(10696..12435)
                     /locus_tag="PLD76_00155"
     CDS             complement(10696..12435)
                     /locus_tag="PLD76_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU26452.1"
                     /translation="MILFKKLLLSFVGIFVFFAAIIFIINVQYEKHMVKARYTETLQF
                     YTEIIDLNTKTARKTEIPNALVDELQKKDVYFSIYNTSGECIYSTPMNDSVIYNVSER
                     AFGMITEKNTPEHTLVVRYDQTKSSYFFYGKKYDTHTILASIPYEYIISDVWKIRNFT
                     ASFFMFFVLSLSSLAFYFVSNKLLHTVKNLKKVILKMENKEDFDDDLLFLNKEFKEIS
                     SFIVNTYKNLTATKDALSLEKEKLFKHLQISQEGLAIYTFDRKEILANENFIQYINLI
                     SNKKISTSDSIFSLAEFSPITSFLNENQYNQNNPNEVTDKIVEIDKNDYTLLVRCIIF
                     QDKTFEISINDISKQVQNNLIKKQLTQNISHELKTPVSSIQGFMETLIENPDMEQEKK
                     DFFIQRCHAQAVRLSYLLRDISLLNKLDEGSYTFEKEKIVLNDIVEGVLKDVSLELEQ
                     KNIRVEVFLKHNMTIMGNYSLLYSIFRNFIDNSLHYAGNDISIIINCYRTDKDFYYFS
                     YADTGVGVSENHLTRIFDRFYRIDKGRSRKMGGTGLGLAIVKNAIAFHQGRISAKPHE
                     TGGLEFVFTLRKE"
     gene            complement(12451..13149)
                     /locus_tag="PLD76_00160"
     CDS             complement(12451..13149)
                     /locus_tag="PLD76_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU26453.1"
                     /translation="MSTFRILVVDDEEDLCDILQYNLEKDGYIVDIAYSAEDALKKDL
                     TQYNLFLLDVMMGAMSGFKLGSMLKKDETTSHIPIIFITAKDTETDTLTGFTIGADDY
                     ITKPYSVKEVVARVRVVLKRTTEQKDGTPMSKDKILRFEQLFVNLTTKTTLIDKQEIN
                     LTKKEFEILSLLMSQPNNVFSREDILQQIWNNADVTDRTIDVNITRLRKKIEPYDKNV
                     VTRLGYGYCFETSN"
     gene            13420..14187
                     /locus_tag="PLD76_00165"
     CDS             13420..14187
                     /locus_tag="PLD76_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU26454.1"
                     /translation="MNRKYRLGLVLSGGGAKGFAHLGVLKAIEEKGLKPDIIAGVSSG
                     ALMGLLYADGKSLDEIMKFFEKASLFDSISPTLPKLGLMNPSKFKRQLRSFLTAKSFE
                     ELAIPFVVNATNLTDGKMTVFRTGESLVEAVIGSSSVPLLFTPQIIEGKQYVDGGVFC
                     NMPVSPIRDECDYIIGVHVNPICTETKAESIIEIAERVLHYAIQSSTINEKKLCDVVI
                     DVKEVKKYGMFNLDKKQEIFDYGYTKAKEVLENFSIE"
     gene            complement(14190..14723)
                     /locus_tag="PLD76_00170"
     CDS             complement(14190..14723)
                     /locus_tag="PLD76_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU26455.1"
                     /translation="MISLEFISHNNHPLLPSIQSIYEEAFPPEEKVDFSSLLSHKFTD
                     DCAFKLYAILDKNETCGMLSIVEYTSFVFIYYLAITPAKQGLGIGNKVLELIKKESSK
                     PLILEVEHATQDISKRRIEFYVRNGFHVNQYPYFQPALHQHTDAVPMHIMSYPHALSE
                     EDFLTVKTALYEHVYCV"
     gene            complement(14816..16177)
                     /locus_tag="PLD76_00175"
     CDS             complement(14816..16177)
                     /locus_tag="PLD76_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU26456.1"
                     /translation="MKTWNYLLLTAVLLLTACSAKEKEPEIIKYTVTIEQPEGGTITV
                     KQGNTVIENGTEIIKGTSLTVTATPDEFYTLESVNVSSPFELTQDTTITAVFEPMVMY
                     TVTIEQNEQGTITVLNKGNVVNSGDQVPENTELSVIAFGNKSHVFSNINTTPYFVLTQ
                     DTTITASFEAIDEQEKYIIGSYNIRYYTRGDTGGKFWNNRKQLVFNLINKYNYDVCGI
                     QETTNTQAVDFLSQLPEYTYIGYGRDNGKEYTDGGIGEQTALIYKTSRFNELAKGRFF
                     LSNTPDSISKLPTSTFNRMVVWVKLEDKQTQKVFFLFSTHFDHPTTQTGINTREQQAT
                     IAKEQIVALTEGYPVIFTGDFNCEPTEPAYTILSGEWHDAYETTPTLRQGGYINKEHT
                     YTGLYSSNDKASKRIDYVFTNDKVDIKSYIAADEKLAHTTYPSDHLPILIECSIQDAV
                     DTE"
     gene            complement(16210..>17932)
                     /locus_tag="PLD76_00180"
     CDS             complement(16210..>17932)
                     /locus_tag="PLD76_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU26457.1"
                     /translation="LDEGRITFRRDITLSFLEQDPSYNPEQTILDTFFSSNNETIRLI
                     AQYEAYISKNENLDSPEFQELVEQMEIRKAWDYDIKIKQILAQLKITDFDKKIKHLSG
                     GQLKRVALANALITEPDLLILDEPTNHLDLEMTEWLEEYLIRSGLTLLMVTHDRYFLD
                     RVCTDIIEIDQKQLYAYKGNYSYYLEKRQDRIDAQNAELARANNLFRKELDWMRRQPQ
                     ARATKAKSRIDSFYDLEKKVAQKRQDESVKLQMNATYLGSKIFEAKYISKQFDDFVLI
                     KDFYYNFSRYEKMGIIGKNGTGKSTFLKLLLGIIKPDSGSFDIGETVSFGYYSQDGLT
                     FDEQMKVIDVVRDIAEEINIGNGKKLSASQFLQHFLFTPETQHNYVYKLSGGEKRRLY
                     LCTVLMKNPNFLVLDEPTNDLDIVTLNVLEEYLANFKGCVIVVSHDRYFTDKVVDHLL
                     VFQGNAVIKDFPGNYSDYREWKEQQDAIDAKQAEIKQKNKVEEPKRSVNRTRKLSFAE
                     QREYSLLESDIENLETEKKDLETMLSGGNIPQQELIKASERIAEVIKLIDDKTMRWLE
                     LSEYTNN"
BASE COUNT         5749 a         2886 c         3310 g         5987 t
ORIGIN      
        1 aatactgcaa ccgactggtc aaaggtgttg gttacggatg agtttgatac cgatcaaatt
       61 ttaaataacc gtttttttgg gttggttcgt attggatctg tacgcgacgt ggtaataaaa
      121 caccacgatt tacagttgcc cgtaggtatt accaatagta tcgtagtatc gtgtgatata
      181 ggcgatgatg tagctattca taatgtaggt tatttatcgt actatattat tggcgatagg
      241 tgcattattt ctaatgtaaa cgaactaaat acatgcaacc atgcaaaatt tggtaatgga
      301 atagtaaaag aaggagaacc cgaaaaaata cgtgtttggc tcgaattaat gaacgaaact
      361 gggtgcagag gagtagctcc ctttgatggt atgattgctg ccgacgctta tatgtgggca
      421 cgatacagag atgatgaggc tttaatgaaa gcattaacgc agattactca aaatagcttt
      481 gatacccgtc gtggctatta tggtaccata ggcgatgagt gcgtagtaaa aaattctttt
      541 gtaattaaag atgtaaaagt aggaactcat tgttatatta aaggggcaaa taagcttaaa
      601 aatctaacca tcaactcgtc tattgaagaa ccaacacaaa ttggcgaagg ggtggaacta
      661 gtaaacggta ttattgggta tggctgtcat attttttatg gatgcaaagc ggtgcgtttt
      721 gttatgggaa ataactcaaa tttaaagtat ggagcccgac taattaattc gttcttagga
      781 gataattcaa caatatcgtg ttgcgaagtg ctcaataatc ttatattccc agcacacgaa
      841 cagcatcata acaactcctt tttggtagct gctttggtta tgggacaaag caatttggct
      901 gcaggggcaa ccattggttc caatcacaat agcagggcta atgataacga agtacaagcg
      961 ggtagggggt tttggcccgg attatgcaca agtattaagc attcttgtcg ttttgcctct
     1021 ttcgtaatgt tatccaaagc cgattacccc gccgaaatgt atattccttt cccattttcg
     1081 ttgctcaaca acaatgtttc taaagaccaa ttagaagtta ctcctgcttt ttggtggatg
     1141 tataacatgt atgccctaga acgcaataca tggaaattta aaggcagaga tggtcgtaaa
     1201 agaaaagtgc aacacatcga attagaagcc ttagctcctg attcggtaga ggagattttt
     1261 gatgccctgc acttacttga actgtgggta ggaaaagcgt tttaccgtaa gcaacaacaa
     1321 aaccttagcc aacttaccga tgaggcaatg caacaaaaag gaaaagaatt attgttttcg
     1381 actgccgaag ctgtaaatgc cttagaggtt ttaggcgaag gaatggagaa aacaaagcga
     1441 aaagtgctta tcatcaaagc atatcaatcg tatcatgcct atagggatat ggtgtattat
     1501 tatgcagtaa acactatttt taactggatg caacacgata cttcgcgtac ctttgctgat
     1561 atggtagttg ctttacaagg gaatagagaa accaaatggg ctaattttgg tggtcaattg
     1621 atgcgagaac aagatgtaga cgctttgcgt caagatatag ggaatggcaa actaacaact
     1681 tggaatcaaa tacaccaacg atatgatagt ttgtgggagc aatatccact acaaaaacaa
     1741 caacatgcgt atgcgtcctt gtgctctgtg ttaggcgtga gtaacctaac tcaaaagcaa
     1801 tggctagaag tattggacaa atcgcttgtg atacaagagt ttgtaagaga ccaagtgtac
     1861 ttgtcgcgca aaaaggattt tgataatcca tatcgcagaa tgacatttag aaacgaagcc
     1921 gaaatgaccg ctgctattgg aactatcgag gacaatggtt ttgtaaagga ggtacgtcaa
     1981 atgacagtaa aatttaatga tttagtaaaa gaaatcaaac aaagaggtta atcatctcta
     2041 ggtatttata cgttaaaaaa acgcaaaaaa atgaatttaa cagacaaaaa gtacgcaaac
     2101 tacgcactgg ttcaagaaat gatggagaca gtgaaggtag tacaaaaatt taatgcccaa
     2161 caaacaaaaa atgtggcatc aaccattcaa tcagtaggca aattattttt aacaggagaa
     2221 ggttctagca gaatttttcc agccaaaaat gccattcgta aatctctaac aaaaggattg
     2281 ccagtaagca tagctaccga tggttctcgt cagtcggcac aatatgattt gagtaagttt
     2341 gctgtgtttt gtgcctctaa ttcgggcaga accaaagagg tggtattgtt ggcaaaacaa
     2401 ttagctgcta ttggtaatgt caaccgtttt gcattaagtg caaatagcgg aacattgtta
     2461 gaagaagttt gtaccgaaac atttgtactt acttgtggct gggaacaagc tgtagctgca
     2521 accaaaagtg tagtagaaca agccttgttt tatgagtcaa ttttgtggca tatcgaacaa
     2581 acaggtactc caaacttagc tgatttagct gcaaaaattg aacaagcatt aacgtttaca
     2641 attgattcat ctattgtgca agctgcggca aaagcaccta ctatctattt tgctggttat
     2701 aacgatggtg tagccgaaga actaacatta aaaaccaatg aaattacacg taaaaaatca
     2761 gatttccttg aaggtaccta tgctgtacac ggtattgaag aggtaatgga ggctcaagat
     2821 gtggtatttt taattgaccc aatagaagag gaaattcaaa aatttcaaga agtattagaa
     2881 caaggagtat gcttaaaaat cattgcaatt gcatcaagac ctacaccttt tactaccatt
     2941 ttagtgccag atgcaggagc cttgtctcct tatgtttatt tgtgtgctgg ttggaattta
     3001 ttggtagaaa ttggtcttgc tttaggcatt gatttggata aaccagaacg tgctcgtaaa
     3061 gtaggaaatg agttgattgt gtaagatatg ttatatactg aaataggggt atgtcgatac
     3121 gtaatacctc tatttttttg ttttttgagg gtaagcttta ttctataaac aaaaaatcgg
     3181 tttaagcttg ttaattattc tgatgatata aaaagactta tgatttatga attcccaaat
     3241 aatagtgtat aaagcccatt acctccatac ttaatgcaat agcaatatgt ataatgacgc
     3301 caccccaaat atgtcgtgtt tggtaagcta aaactcccaa tatatatcct ccaaatatcg
     3361 aagaaattgt ttcgcctata ggtttcccaa aatgcaacac acagtacata gccaccattg
     3421 gtaatatagc atgtctacct aaaatacttg ccatgccaat aattaatgca cctctcataa
     3481 ctaactcggt aatgacataa aatgaggcat acacactttc aaagattatg gtatgttgcc
     3541 agttctccca atgaaatata ccttcgtacc accagtaacg atagatggga tatgcacgca
     3601 taaaatctgg tgtaaaagaa atccatacca aaaatggcaa aaggaataag aataaactca
     3661 tataagcagc tatgtgttta ttcgaaatgg ttaatccata caatcctttt tcttttctat
     3721 cccataaata tttagcaata ataagcaatg gtagtgtggt aatattagct ttgaagaaga
     3781 aaattagtcg aattagaaaa tggttttcta aatatgtaaa gctttcaatt tttaaatcct
     3841 gataggtgaa atttcctacc actacagcaa agcttacaat aaaaaagatt gcttttacgt
     3901 aatataaagg ttgtgttaaa atagcagttt gtttacgtat aattagtaca ggaatggata
     3961 ccataaagta agcagccata taaaatatgc taaaatagag catagaccaa cccgaagaat
     4021 agcttggtac caaaaaggtg tcatacaaat tataggtata attaacataa atacagatac
     4081 cacaaaatgt caatgtataa agatatgaca cagcgtcaaa gtctgattta ataaaagaaa
     4141 taacttcacg tagaagagct ttaatttttt gtatcattca ggctaaggtc aattcatttt
     4201 aaaagtttgt caaaagtaca aaaaagtgta aattatttgc tacttttgtg ggcaaaattg
     4261 taaaaactta tgatagcaga aaaaatcctt attcttgatt ttggttcaca aaccactcaa
     4321 ttgattgcac gcagggtgcg cgaattaaat acatattgtg agattgtgcc ttataataaa
     4381 ttccctgttg gcgataccag tatcaaaggc gttattttgt caggaagtcc tttttcagta
     4441 tatgaccagg ctgcatttaa aacagattta tcgcttttta gaggacaatt gcccgtttta
     4501 ggtatttgtt atggagccca gtttattgca caacaatctg gaggaaaagt gcaacctgca
     4561 aatagtagag agtacggaag agcaaagttt tctgtctgtc aatcaaataa tccgttatta
     4621 aaaggtattc aagaaaaatc gcaagtatgg atgtcgcacg gtgatacaat tacagatata
     4681 cctgctaatt tctcaattat agcaagtaca cacgatgtag aaatagctgc ttatgccgta
     4741 cagggagagc aaacgtgggg tgtgcaattc cacccagagg tgtttcatac tactgacgga
     4801 agcaaattgt tagacaattt tttaaacatc tgtggatgta aaaaagattg gtcagctgct
     4861 tcttttatcg aaactactgt ggcagagtta aaagcacaat tgggtgatga taaagtagtt
     4921 ttaggtcttt cgggaggtgt tgatagttct gtagcagcgg tactgttaaa caaagccatt
     4981 ggcaaaaatt taacgtgtat ttttgtagat cacggtttgt tgcgtaaaaa tgaattcgaa
     5041 acagtattag atgattatgc tcatttaggt ctcaacgtaa ttggggtaaa tgtaaaagac
     5101 cgtttttatg caaaattagc tggtgtaact gatccagagc aaaaacgtaa aataattggt
     5161 aaagaattta tagaggtatt tgatgaagaa gctcataaaa tacaagacgt aaaatggttg
     5221 gctcaaggca ctatttatcc tgactgtatc gagtctcttt ctatcacagg cacagttatt
     5281 aaatcgcacc ataacgtagg tgggttacca gagaaaatga acttgaaatt gtgcgaacca
     5341 ttacgtttac ttttcaaaga tgaggtgcgt aaagtaggta atgagctagg aatgatgtcg
     5401 caccttatta agcgtcaacc atttccaggt cctggtttgg ctgtacgtat attaggtgat
     5461 attactgccg aaaaagtacg tatattacaa gaagctgatg atatttttat caaaggattg
     5521 cgtgaatgga atctttacga ccaagtatgg caagctggtg ttatcttatt gcctgtgcaa
     5581 tctgttggtg taatgggtga tgaacgtacc tacgaaaatg cggtagcctt gcgtgctgta
     5641 acatctactg atgctatgac agccgattgg gcacacttgc cttacgagtt tttaggcaaa
     5701 atgtcaaacg aaatcatcaa taaagtaaaa ggagtaaata gagtagtgta tgatattagc
     5761 tccaaaccac ccgcaactat tgaatgggag taatataagt ttaaaaaaaa ttaaaaaaag
     5821 aacatttctt tgttttttta catagctttg tggcagtaaa taataacaaa aaatatacaa
     5881 ctatgaaatc gaaacttttt atcattggcg ttttagttaa tctttttatt ggttttgcag
     5941 gtatagccta tgctcaacaa agtgcaaata attctaattc aacctcatct gaggtgtcgg
     6001 cgatggcgtt tgaaaagacc gttcatgatt ttggaacttt taatgaggca gatggtaaag
     6061 ttgaaactgt atttgtattt aaaaatacaa gcaaagagaa tcttgtagtt agtagagtgc
     6121 aagcttcttg tggatgtact actcccgatt ggtcaaaaga acctattgcc ccaggaaaaa
     6181 aaggttttgt taaagcatca tacaatccag ctggtcgacc tggtaatttt aacaaaagtg
     6241 taactgtaac gagcaatcaa ggaacacaag ttttatatat caaaggacaa gttgtgccaa
     6301 aagccaacta aggaacaata atatttttca aatttgttta acccacattg aatgcagtgt
     6361 gggttttcat ttttttatgt acctttgtac ctatattatc acgttctatt tgtttgctgt
     6421 actatccaat gggcaatgcc tccattaaat atgcataaca tagataataa ccaataaata
     6481 tgaaatattt tacactactt tgtttgactt ttatccttat ttatggcaaa gtatatgcta
     6541 tttcttttga tgcactaaca cacgactttg gaacaatata cgaagaaaaa gggactgtgt
     6601 caacaacatt ttctttcaaa aataagtcaa ataaactact tgttattgaa agaattgtgc
     6661 cagcatgtgg ttgtagtaat actcatatta gtagtagaaa tctagctgct ggtgagtctg
     6721 gtaatattgt tgttgagttt gatccatcag aaagaccagg aaattttgat aaaagcattt
     6781 acgtttatag taatgactct actgtgcgtt tgtcaataca gggaaaggta ataccaagca
     6841 aacaaaccat agccaaaatg tatccgtacc gttttcaatt tagccgtgct gataaaaata
     6901 ccttgagctt tggctctatt cttcacacac agcaaagtac tcaatatgtg aattttataa
     6961 atgactcaga taaacctgta aaactgactt ttttacaact ccctccattt atttctactg
     7021 attcattatt ttataatcta ttacctggta atgaacagat tattcctttt catatctcag
     7081 tactaggaca taatgtgtgg ggagaaaaaa atgaaaaatt agttgtagtt gaaaatggca
     7141 aaccttcaaa tgctatacaa atcaatgtaa cagttaacga ggatttttct atcttatccg
     7201 ccaaagaacg ttctcgtatg ccaaaaataa agctatcatc taacgaaatt atttttggta
     7261 agacaaaaca accaagcatt gaaacaaaag agttaactat aaccaatata ggtcatgggc
     7321 aattacttat acgaaagatt gaactaacag accctgcttt acgtgtttcc attactaaaa
     7381 cgaaactaaa taggaataaa cgtgctcagg taaaaatatc gttgttgtct gacaaatcat
     7441 tgggcgaaaa agaacatatc tttaacattt taaccaatga tccaatacat ccagtgcaaa
     7501 ccattattgt aaagtcatct atatataagt aaagccatga atcacataga aaacaatgaa
     7561 gaatatggag gcttggtagt aaacaaggga attgctcaac caccaactgt aaatccaaat
     7621 ttggctagtt cgcgaaaaaa acgtactatt tacacagcta acgattacct agatggtata
     7681 attaagggca acagaactat attgagtcag gctgttactt taatagaaag tacaaagccg
     7741 gaacactatg ctatggctca agagttgata gaaaaatgtt tgccatatgc caaaaaatct
     7801 gtgcgtatag gtattacagg tgtgccagga gcagggaaaa gtacatttat cgaagcattg
     7861 ggaatgtatg ttgtaaatca gcacaaaaaa ttggcggtat tagctattga tccaagcagt
     7921 cagcgaacta aaggaagtat tttgggtgat aaaacgagga tggaagagtt gtcagtacac
     7981 gaaaatgcct ttatacgtcc ttcaccatct gctggttctt taggcggagt agcacgtaaa
     8041 acgcgtgaaa ccatcgtgtt gtgcgaagcc gctggatttg atactatttt tgtagaaaca
     8101 gtaggtgtag gacaatctga gattgctgtt cattctatgg tagatttctt tttgttactt
     8161 caaattacgg gtgctggtga tgagttgcaa ggtatcaaac gtggaattat ggaaatggct
     8221 gatggaatag taatcaacaa agcagatggg gcaaatgtgg ataaagccga aatggcaaaa
     8281 acacaataca aaacggcact atctttgttt ccaatgcacg aatcaggatg gaaaccccag
     8341 gtagtaacct gttccgcatt ctacaaaaca aatattgatg ctatttggac gatgttgatg
     8401 caatatattc aatttactca acaatcagga tattttgaaa caaaaaggca tcagcaagcc
     8461 agttactgga tgtatgaaag tatcaatcaa gaattaagaa gtagttttta tgcccactca
     8521 gaagtgaatg cattactaaa agaaatggag gtaaaagtta caaatggaga aattagctcc
     8581 tttattgctg ccaaacgttt acttgatgta tatgacaaaa tccggaaata gacaaataca
     8641 actttctttg tgaaatcatt tggtaattat attttcatcc ttgttatgtt ggtggcttgt
     8701 tctcaatata aaagtaaatc tatacttcaa ggtacaatca ctgatagtcg gtataaaaaa
     8761 gtatatttat tgcaacaaat aacgcctaca tcatgggaaa tgttggactc tacttctatt
     8821 gttgataatc attttatgtt tgctgattta ccttctgaat acaatcaact atttgttcag
     8881 ttggataata caatcccttt tgtagtgttt gttgataagg gaactattga gttagctata
     8941 ccagcccaaa atgtacatga aacaaaggta aaaggatctt ctttacatga tgaatacatg
     9001 atgttacaag atagtattga ttttttattg catcaacgtt tgttgtttta taaagatgct
     9061 attgttgcac aaaatgatgg aagacagaca gatgcaacaa tacttcaaca aaaatatatc
     9121 catagcaaaa ataatgtatt tcattttctt cagaaacaaa aaggagcaaa tcctacttct
     9181 gctcctttgt tatatataat taaaacatat aaaccgtatt ttaccgacac ggaatatcga
     9241 acattatctg cttctttaga gtaatttatt ctttatcggc aactccttca acggtaattt
     9301 gacgtttgaa tgtttcacct agtgacatca gtgtttctgt gctagctatt ccttttatac
     9361 tttgtagttt gtcatgaata atgctcaaaa aatcactatt attgcgtgca taaattttaa
     9421 taaacatcgc atattttcct gttatataat gacattccac tacttcgggt atttttagta
     9481 gttcgtcgac tacttcttta aagaaattag catctttcaa aaatattccc atgtatgcac
     9541 atgtattgta accaattttg actgggtcaa ttataaattc ggaacctttt attacaccta
     9601 ctgaaattaa tttttgcaca cgttggtgaa tagccgcacc agatacattg cattcacgag
     9661 caacttctaa aaaaggcgtg cgtgcatttt tggaaattaa ttgaaggatt ttttcgtcta
     9721 aactgtctaa ttgatactgt gccatgcgta ttttgctttt aaattatatg caaaaatact
     9781 atttttattt catattacgt attttgttat cattttttag tattaaaatt atatatacca
     9841 atgggttact tttttgttgt ctatcttaca atttgttact ctacttgagt tgttcggctt
     9901 caaacttcct aatctttttt taattaattg catttatttt ggtaaaaaat gtatctttgt
     9961 gccttgaaat attgttgtaa agtattattt atatggcaac taagcgttta aaggaaatta
    10021 aacctattga ggagactgcc gatatttgga cactgttaag ctcagaccaa gcccattatt
    10081 tgcaaactaa cttacaggtt ataagtgcca aaaaaaatga agtaatttat tcagacggtg
    10141 aaattccttc tcatttattt tgtcttgtta gtggcaaggt taaagtgtat aaacaaggca
    10201 taggaggacg taatcagatt gtccgcgtta taaaacccaa tgaattattt gggtataggg
    10261 caatgtttgc tctcgaggct tatgtaacct gtgcttctgc tattgagcct tgtgttattt
    10321 atgctattcc agaggatgtt attgtttcta ttataaaaaa taatgctcaa cttgcgtttg
    10381 cttttatcca atttttagct gttgatttgg gaatttctga tgcaagaaca gtaaccctta
    10441 ctcaaaaaca tattcgtggt cgattagcag aatcattgct gtttttacgt gataattatg
    10501 gcgttgaatc tgatggtatc actctatcta tttatctttc tagggaggat ttagcaagct
    10561 tgtcaaatat gaccacttcc aatgctattc gaacattatc gttattttca aacgaaggaa
    10621 tgattgttgt agagggtaga aaaattaaaa tagtagatgt agagtctctt gtaaaaatca
    10681 gtaaatttgg ttgatttatt cttttcgtaa cgtaaaaaca aattctaatc cccctgtttc
    10741 gtgtggtttt gctgaaattc taccttgatg aaatgcaata gcatttttaa caatagctaa
    10801 acccaatcct gttcctccca ttttacgaga acgtcctttg tcaatacgat aaaaacggtc
    10861 aaaaatacgg gttaaatggt tttcagatac ccccactcct gtgtcagcat acgaaaaata
    10921 gtagaaatcc ttgtctgttc ggtaacaatt aatgataata ctaatgtcgt tgccagcata
    10981 atgtaggcta ttatcaataa aatttctaaa gatggaatat agtaacgaat agttgcccat
    11041 tattgtcata ttgtgcttta gaaacacttc tactcgaatg tttttttgtt ctaactcgag
    11101 cgaaacatct tttagtactc cttcaacaat atcatttaat acaatcttct ctttttcaaa
    11161 tgtgtaagaa ccttcgtcga gtttgttgag taacgaaatg tctcgtaaca aatagcttaa
    11221 acgaacagct tgagcatggc aacgttgaat aaaaaaatct tttttttctt gttccatgtc
    11281 gggattttct atcaatgttt ccataaaacc ctgaatacta ctaacaggcg ttttcagttc
    11341 gtgagaaata ttttgtgtga gttgcttttt tattaagttg ttttgaactt gtttggagat
    11401 gtcattaatt gaaatctcaa aggttttatc ttggaaaata atacatcgaa cgagcaaggt
    11461 ataatcgttt ttgtctattt ctacaatttt gtctgttacc tcattgggat tattttgatt
    11521 gtattggttt tcatttaaga aactagtgat aggggaaaat tcggctaatg aaaaaatact
    11581 atctgatgtg ctgatttttt tgtttgaaat caaattaatg tattgtatga aattttcgtt
    11641 tgccaaaatt tcttttctat caaaagtgta aatcgctaag ccttcttgcg atatttgaag
    11701 atgtttaaaa agtttttctt tctctaacga taaggcatct ttggtggctg taaggttttt
    11761 atatgtgttt acgataaaac tagatatctc tttaaattct ttgtttaaaa aaagtaaatc
    11821 gtcatcaaaa tcttctttgt tttccatttt taaaattacc tttttgaggt tttttacggt
    11881 atgcaatagc ttattgctta caaaataaaa cgctaagctt gataaactta gtacaaaaaa
    11941 cataaagaat gaagccgtaa aattacgaat cttccaaacg tcacttataa tatattcgta
    12001 tggaattgag gctaaaatcg tgtgtgtatc atattttttt ccataaaaga agtaggagga
    12061 ttttgtttgg tcgtaacgta caactaatgt atgttctggt gtgttttttt ctgtaatcat
    12121 tccgaatgca cgttcagata cattgtaaat aaccgaatca ttcattggtg tggaataaat
    12181 acattcgcca ctcgtattat aaatagagaa gtacacatct tttttttgca attcatctac
    12241 cagtgcgttg ggaatttcag tttttctagc agtctttgta tttaaatcaa ttatctcggt
    12301 ataaaattgt aatgtttctg tataacgtgc ctttaccatg tgtttttcgt attgcacatt
    12361 gataataaaa ataatagcgg caaaaaacac aaaaatacca acgaatgata ataatagttt
    12421 tttaaacaaa atcatggttg tggaggcttg ttagtttgat gtttcaaaac aatatccata
    12481 tcctagccga gtaacaacat ttttatcata aggttctatt ttttttcgca gtcgagtaat
    12541 gtttacatca atagttcggt cggtaacatc agcgttattc catatttgtt gtaaaatgtc
    12601 ctctcgagaa aaaacattgt tgggttggct cattaataag cttaaaattt caaattcttt
    12661 ttttgttaaa tttatttctt gtttatcaat gagtgttgtt ttagtagtta agttcacaaa
    12721 caattgctca aaacgcaaaa ttttatcttt cgacatgggg gttccgtctt tttgctccgt
    12781 agttcgtttt aatacaactc gcactctagc aactacttct ttcactgagt aaggttttgt
    12841 aatataatca tcagccccta ttgtaaaacc agttaaggtg tctgtttctg tatctttagc
    12901 tgtaatgaaa atgattggaa tatgcgaggt tgtttcgtcc tttttgagca tagaaccgag
    12961 tttaaagccc gacatagctc ccatcattac atccagtaaa aatagattgt attgtgttag
    13021 gtcttttttt aatgcgtctt cggcagaata agcaatgtct acgatatatc cgtctttctc
    13081 taaattgtat tgtagtatgt cgcacaaatc ttcttcgtcg tctactacca aaattctaaa
    13141 tgtactcata gtgcataggt ttttttacaa agatatttat tttattgcta ttttgcacgt
    13201 taagcgataa tattttcttg ctgaatatgt tttagtatag attccataga ggtatatgag
    13261 tcgtaggata catataatca gatgattttg tggtgttagt attcattttg gcaacatgta
    13321 gaatgttata taattgtaat atggcatagt aatttgcttt ttttatagta taatgtacgc
    13381 aaatatagtg tatttttgta caattaaata tattgtgcga tgaatagaaa ataccgattg
    13441 ggacttgtat tgagtggtgg tggagcaaag ggttttgctc atttgggcgt gttaaaagcc
    13501 atcgaagaaa aaggactgaa gcccgatatt attgctggtg taagttcagg tgctttaatg
    13561 gggctattgt atgctgatgg taaaagctta gatgaaatta tgaaattttt cgagaaagcc
    13621 tccttgtttg actccatatc gccaaccctg cctaaattgg gtttaatgaa tccttcaaaa
    13681 tttaaacgtc agttacgcag ttttttaacg gcaaagtcat ttgaagagct tgctattcct
    13741 tttgtagtga atgcaacgaa tcttactgac ggcaaaatga cggtgtttcg tacaggtgaa
    13801 tctttagtag aagcagttat tggctcttcg agtgttccgt tattatttac tccccagatt
    13861 atcgaaggga aacaatatgt ggatggaggt gttttttgta atatgcctgt ttccccaatt
    13921 cgcgatgagt gtgactatat tattggagtt catgttaatc caatttgtac cgaaaccaaa
    13981 gccgaaagta ttatcgaaat agcagagcga gtacttcatt atgccataca atcaagcacg
    14041 attaacgaaa aaaaattatg cgatgttgta attgatgtaa aggaagtaaa aaagtatggt
    14101 atgtttaatt tagataaaaa acaagaaatt tttgattatg gctatacgaa agctaaggaa
    14161 gtattagaaa atttctctat agagtaagac tatacacaat atacatgttc atataatgct
    14221 gttttgacag tcaaaaaatc ttcttccgaa agtgcatggg gatagctcat gatatgcatg
    14281 gggacagcat ctgtatgttg atgcagtgct ggttgaaaat aggggtattg gttgacgtga
    14341 aacccattac gtacataaaa ctcaattctt cgttttgaaa tgtcttgtgt agcatgttct
    14401 acttcgagaa taaggggttt tgagctctct ttttttatta actcaagtac tttattccct
    14461 atacctaatc cttgttttgc aggcgtaatt gctaaataat aaataaatac aaaagaagta
    14521 tattctacta tactcagcat gccacaagtt tcatttttat ctaggattgc atatagctta
    14581 aaggcacagt catcagtaaa tttatgcgac aataaagaag aaaaatctac tttttcttct
    14641 ggcggaaagg cttcttcgta aatagattga atgctgggca agagaggatg attgttatga
    14701 gagataaatt caagtgaaat cataaactat ataatttacc gaaaatggtt actctattat
    14761 agtaagtctt atatacaaat aggctgtctt tgacagccta tttgagattg taaaattatt
    14821 cagtatctac agcatcttga atgctacatt caataagtat aggtaaatgg tcagaaggat
    14881 aagtagtgtg agctaatttt tcgtctgctg caatgtaaga ttttatatct actttatcat
    14941 ttgtaaaaac ataatcaatt cgttttgaag ccttgtcatt gctgctgtat aaacccgtat
    15001 aggtatgttc tttgttaata tatccccctt gacgcaatgt aggagtagtt tcgtaagcat
    15061 catgccattc tcctgataat atagtatatg caggctctgt gggttcgcaa ttaaaatctc
    15121 ctgtgaaaat tactggatag ccttcggtta aagctactat ttgttctttg gcaatagtcg
    15181 cttgttgctc acgtgtattg atgcctgttt gtgtagtggg gtggtcgaaa tgtgttgaaa
    15241 ataagaaaaa tactttttga gtctgtttat cttcgagttt tacccatact accatacgat
    15301 tgaatgtaga ggtgggaagt tttgaaatcg agtctggtgt gtttgataaa aagaaacgtc
    15361 cttttgctaa ttcattaaaa cgactagtct tataaattaa ggctgtttgt tcaccaattc
    15421 ctccatctgt atattctttt ccattatctc ttccatagcc gatatacgtg tattcaggca
    15481 gttgacttaa aaaatcaaca gcttgtgtat ttgtagtttc ttgtatgcca catacatcgt
    15541 agttgtattt gtttattaaa ttgaaaacta actgtttgcg gttgttccag aattttccac
    15601 ccgtatctcc tcttgtgtaa taacgaatat tatacgaccc aataatatat ttttcttgtt
    15661 catctattgc ttcaaaagat gctgtaattg tggtgtcttg tgttaataca aaataaggtg
    15721 ttgtattgat attgctaaaa acatggcttt tattgccaaa agcaatcaca gaaagttccg
    15781 tgttttcagg cacttggtct ccactattta ctacattccc tttattcagt accgtaatag
    15841 ttccttgctc gttttgttca atcgtaaccg tatacataac cattggctca aatactgctg
    15901 taattgtggt atcttgggtt agctcaaaag gtgaacttac gtttacactt tccaaggtgt
    15961 aaaattcatc aggggtagct gtaacggtaa ggctggttcc ttttattatt tcagttccgt
    16021 tttcaattac ggtatttcct tgttttacgg taatggttcc gccttcgggt tgttcaatag
    16081 taacggtgta cttgattatt tctggttctt tttccttagc actacatgct gttaaaagaa
    16141 gaactgctgt aagcagtaag taattccatg ttttcatacg tataggttat taattgatta
    16201 aaaagttatt taattgttgg tgtattcgct tagctctaac catcgcattg ttttatcgtc
    16261 aattagtttt atcacttcag caatacgttc agatgctttt ataagctctt gttgaggaat
    16321 atttcctcct gataacattg tttcaaggtc ttttttttca gtttccaaat tttctatgtc
    16381 tgattctagc aatgaatatt cacgttgttc ggcaaaactc aactttcttg tacggttaac
    16441 agagcgtttg ggttcttcaa ctttattttt ttgtttaatt tctgcttgtt tggcatcaat
    16501 agcatcttgt tgctctttcc attctcggta atcggaataa ttgcccggaa aatctttgat
    16561 aacagcattg ccttgaaata ccaataagtg atcaactacc ttgtcagtaa aatacctatc
    16621 gtgcgaaacg actatcacgc agcccttaaa attagctaaa tattcttcca atacatttag
    16681 ggtaacaata tccaagtcgt tggtaggctc gtcgagcacc aaaaaattgg ggtttttcat
    16741 caaaacagta cataggtaaa ggcggcgttt ttctcctcca ctaagtttgt acacataatt
    16801 atgttgtgtt tcgggtgtaa acaaaaaatg ttgcaaaaat tgggatgcac ttagtttttt
    16861 tccgtttcct atatttattt cttcggcaat gtcgcgtact acatcaatca ctttcatttg
    16921 ttcatcaaac gttaacccat cttgactgta gtagccaaaa gaaacggttt cgccaatatc
    16981 aaatgagcca ctgtcgggtt taataattcc taaaagcaat tttaaaaaag tagattttcc
    17041 tgtaccattt ttaccaatta tgcccatttt ctcgtatcgg gaaaaattat agtaaaaatc
    17101 ttttatcaac acaaaatcgt caaattgttt gctaatatac ttggcttcaa aaatttttga
    17161 acccaaataa gtggcgttca tttgcagttt taccgattca tcttgacgtt tttgtgccac
    17221 ttttttttct aaatcataaa aactgtcaat tctgcttttt gccttcgttg cccttgcttg
    17281 aggttgtcgt cgcatccaat ctaattcttt tctgaataaa ttgtttgcac gtgctaactc
    17341 agcattttgg gcatcaatgc ggtcttgtct tttttctaaa taatagctgt aattgccttt
    17401 gtatgcgtat aactgttttt ggtctatttc tataatgtca gtacacacac ggtctaaaaa
    17461 atacctatcg tgagttacca tcaataaggt taatcccgaa cgaataaggt attcttctaa
    17521 ccattcggtc atttctaaat ccaaatggtt ggtaggctca tctaaaataa gcaagtcggg
    17581 ctctgttatc aaagcattag ccaaagctac ccgtttgagt tgaccgccag ataagtgttt
    17641 aatctttttg tcaaaatccg taatcttgag ttgtgctaaa atttgcttaa tcttaatatc
    17701 ataatcccaa gctttacgaa tttccatttg ttctaccaat tcttgaaatt ctgggctatc
    17761 taaattttcg tttttgctga tatatgcttc gtattgtgca attaggcgaa ttgtttcgtt
    17821 gttcgacgaa aaaaacgtat ccaatatagt ctgttctgga ttgtaagatg ggtcttgctc
    17881 taaaaaagaa agcgttatat cccggcgaaa ggttattctc ccttcgtcta ag
//
LOCUS       DAOSMY010000003        12478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_5055_length_12478_cov_3.766965, whole genome shotgun sequence.
ACCESSION   DAOSMY010000003 DAOSMY010000000
VERSION     DAOSMY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12478
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5055_length_12478_cov_3.766965"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..500
                     /locus_tag="PLD76_00185"
     CDS             207..500
                     /locus_tag="PLD76_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26458.1"
                     /translation="MKSVFIVFNQANTERVEYMLDRLSIRGFTFWENVQGVGSMNGEP
                     RRGTHTWPEMNSAVMTVIPDEKVDDLLIAVKKLDQRNKEVGVRAFVWNIEQMV"
     gene            complement(505..1563)
                     /gene="mutY"
                     /locus_tag="PLD76_00190"
     CDS             complement(505..1563)
                     /gene="mutY"
                     /locus_tag="PLD76_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOU26459.1"
                     /translation="MTFSEIIIDWYQVNKRNLPWRNTKDAYTIWVSEVILQQTRVVQG
                     LAYFIRFIERFPTVIALAQADEEEVLKYWQGLGYYSRARNMHKAAQQVVELYDGIFPT
                     HYPHLLTLQGIGEYTAAAISSFAANEAHAVVDGNVYRVLSRVFGIFLAINSNEGKREF
                     KKLAQSLLPTHQASTYNQAIMEFGALQCVPARPLCSTCPLQVMCYAYTHQHVSSLPKK
                     EKKTTTKTRYFFYLDIRNGEYTYLQKRTQNDIWHNLFEYPLVECDKETSIENVINSED
                     FQEIVEKCEKTSIHSISSEIKHVLSHQIIRTIFIRIHIETDNSLLQSLYKINSRDIED
                     YPISRLMHKYIEKITNET"
     gene            1726..2010
                     /locus_tag="PLD76_00195"
     CDS             1726..2010
                     /locus_tag="PLD76_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009435111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU26460.1"
                     /translation="MTKAEIVNEIANKSGIDKVTVLRVVESFMDEVKDSLASNENVYL
                     RGFGSFVVKQRAEKVARNISRNTTITIPAHSIPSFKPAKTFVEAVKNKII"
     gene            2229..3803
                     /locus_tag="PLD76_00200"
     CDS             2229..3803
                     /locus_tag="PLD76_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOU26461.1"
                     /translation="MNNTLLLDVQSKDISIALMENNTLVEFSKEPRDMSFSVGDIFLA
                     KVKKIMPGLNATFVDVGYDKDAFLHYLDLGSNFHSIAQFTKTLVNDRKKIPSMQKNSG
                     LPDLEKDGFIADILKSGQEILVQITKEPISTKGPRLTAEISIAGRSLVLLPFSNKISI
                     SQKIKSEEERNRLRQLLVSIKPKNFGVIIRTVAEGKKVAELDAELKILEKRWEEAIEN
                     IQKAKQFPSLILKEMSRAETILRDSFNPNFQHIYVNEKTIFEEIQAYVGLIAPEKNDI
                     VKLYTDDIPIFDAFNITKQIKSSFGKITPFKNGAYLVIEHTEALHVIDVNSGNRSKAG
                     NVQETNALEVNLAAVDEVARQLRLRDMGGIIVIDLIDMQENEHKQQVYERMKERMQND
                     SAKHNILPLSKFGLMQITRQRVRPQTLIDTKETCPTCDGNGKIRSSLFFVDMLETKLN
                     EIKNTMKIRKCILFVHPFVYSFINAGIMSIKNKWILKFGLGFKVLPNQSYSYLQYGFF
                     DTDGNEIDVKDTADMA"
     gene            3815..4447
                     /gene="nth"
                     /locus_tag="PLD76_00205"
     CDS             3815..4447
                     /gene="nth"
                     /locus_tag="PLD76_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU26462.1"
                     /translation="MLLKERYKGVIEWFQQHMPVAETELHYTNSFELLVAIVLSAQCT
                     DKQVNLTTPALFKSFPTPQAMAKASFEEVFALIKSISYPNNKAKHLIALSQQLVDKYD
                     GVIPDDAERLQELPGVGRKTANVMLVVAFKKPAMPVDTHVFRVANRIGLTTAKNPLQS
                     EKTLVKNIPNHLLPIAHHWLILHGRYTCLARKPKCDECGIRTFCKFFNKK"
     gene            4508..5977
                     /locus_tag="PLD76_00210"
     CDS             4508..5977
                     /locus_tag="PLD76_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOU26463.1"
                     /translation="MSLNIENILLIGSLLLLVSIIAGKTSYRFGVPTLILFLAVGMLS
                     GSEGLGGIRFDDPQIAQFIGIVSLNFILFSGGLDTHWHQVKPVLWQGITLSTVGVLIT
                     AISLGTFVWLITDFSLVESLLLGSIVSSTDAAAVFSILRSKSLSLKKNLRPTLELESG
                     SNDPMAYVLTIAFLTLVVHQDMSVVSIIPLFLQQMVFGAILGYVFGRISKLIINRIKL
                     DFEGLYPVLVIALMFLTFSATNFVGGNGFLAIYICAVYLGNQNLIHKRTILKVYDGLA
                     WLMQIVLFLTLGLLVFPSHILPVVGIGLLVSVFLIIIARPLSVFLSLLPFRMPIRSRF
                     YISWVGLRGAVPIVFATYPLLAGIDKADMIFNIVFFVSVSSVLLQGTTLGIVAKWLGV
                     ADKKKETTSSSSEVFKAEYPTASMQEIIIAPNAYAVNKTIAELSFPKSANIAMIHRDG
                     NYLTPTGSTVLAANDILVVLSNNEEGLSKAQACLSATSV"
     gene            complement(5985..7328)
                     /locus_tag="PLD76_00215"
     CDS             complement(5985..7328)
                     /locus_tag="PLD76_00215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOU26464.1"
                     /translation="MLLISNGRIVNEGKIYKASILIKDGKIGAIYKDEVPKSIVEKVH
                     KIDADDCWVLPGVIDSHVHFRDPGLTHKGDLLTESKAAAAGGVTSIMDMPNTNPQTIT
                     VQAFEEKCQLASTKSLVNYSFYLGATNSNFEELQKIDPSKVCGIKLFMGSSTGNMLVD
                     KDSLLHRIFEDVKIPVAIHSEDEGTVKANAATYKAEYGENPIPMKYHAKIRSAEACYK
                     STALAIELAKKYGTRLHILHLSTAKELALFDNVSLDKKNVTAEACPHYLLFDENDYDT
                     LGARIKCNPSIKSTQDKEALLAAINTQKIDTIGTDHAPHLLSEKEGDALTATSGMPQI
                     QHALVSMLELAKHGKTTIENVVEKMCHNPAKLYQINKRGYIRKGYYADIVIVQRNASW
                     QVNTDTILSKCGWSPYEGKKFSSKVTYTIINGNVVYSNGEFNEDTKGQALEFTRA"
     gene            complement(7362..8099)
                     /locus_tag="PLD76_00220"
     CDS             complement(7362..8099)
                     /locus_tag="PLD76_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOU26465.1"
                     /translation="MSDSLVLIPTYNEKENIENIIRAVFALPKSFHILVIDDNSPDGT
                     ADIVKRLQVEFSTQLHLLERKAKNGLGRAYIAGFNWALSSAYEYIYEMDADFSHNPND
                     LLRLYEACSQGGADVAIGSRYVTGVNVVNWPMGRVLMSYFASKYVRFVTGLAIADTTA
                     GFKCYKRAVLETIELDKIKFVGYAFQIEMKFTAFKCGFNIKEVSVIFINRELGTSKMS
                     GKIFREAVIGVLQLRWESFFRKFPQKK"
     gene            8233..11517
                     /gene="mfd"
                     /locus_tag="PLD76_00225"
     CDS             8233..11517
                     /gene="mfd"
                     /locus_tag="PLD76_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU26466.1"
                     /translation="MDIKEFSQSLISHRVVKGINQWVESSHKNVCIQGFCGSSCALFF
                     ASSILAKTNTVYILNDAEEAAYFYHDLLQLLSPDDVYYFPSSYKKRVREGDQKDAANQ
                     VLRTEALNDISQKQSWILVTYPEAIAERVIPQQDLSKETLHLAVGEKLDSDFVISLLV
                     EYGFERVDFVYEPGQFAVRGSIIDVFSFSHDVPFRIDFFGDTIDTIRTFDIESQLSIE
                     KKTHISIVSNSVNIEENALVPLFKFFTNKVVYTIKNTPYVVDLIDKINQENDETRFIL
                     STEWIEQWQGNKVIEFGNSPFFSSPHIITLNQNIQPIFHKNFDLLAGSLTEYIAQQYQ
                     IYVLSDNPKQTERLKDIFEDKNFPITFTPINKTIHEGFVDNDLKICVFTDHQIFDRYH
                     KYSLKSQKTKAGKLVLSLKELMQFQVGDYVVHSEHGVGRFGGLIRTEINGKTQEVIKL
                     LYKDDDIIFVNIHSLHRISKYKSKEGEPPKINKLGTTAWVNLKEKTKKKVKDIARDLI
                     ALYAKRKSEKGFAFSPDSYLQNELEASFLYEDTPDQLKTTIALKKDMESDKPMDRLIC
                     GDVGFGKTELAIRAAAKAVADSKQVAVLVPTTVLAFQHFNTFKKRLKKFPCTVEYISR
                     ARKTSDVKLIIEKTKTGAVDILIGTHKLLSKELEFKDLGLLIIDEEQKFGVSTKEKLR
                     QLRSHLDTLTLTATPIPRTLQFSLMGARDLSVITTPPPNRYPVQTELIRFDDDIIREA
                     IEFELARNGQVFFVNNRVSNLPELEMQLKRLVPHARIAVGHGQMDSEKLERVLLDFIH
                     HEYDILLATTIIESGIDIPNVNTMFINNAQYFGLSDLHQLRGRVGRTNKKAFCYLISP
                     SLAILPTDARRRLQAIETLADLGSGIHIAMQDLDIRGAGNILGAEQSGFITDLGYETY
                     HRILDEAVNELKHEEFADVFAEEIEHHMNYVADCMFESDLELLFPSDYVQNISERMDL
                     YRRLDTINNEEDLQHFEKELIDRFGPLPFVSIELIQVVRLRWLCAALGIEKIQMKHGR
                     LTAYFISNPSSPYYQSETFGAMLLFAQTHPRNTQFREINGKRSLLLSGIDTIQKAYTI
                     FKDIRE"
     gene            11631..12368
                     /locus_tag="PLD76_00230"
     CDS             11631..12368
                     /locus_tag="PLD76_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432906.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU26467.1"
                     /translation="MSSNKSIISVSIVAVSIILSVFILSNAFVNRNKTDNTISVTGLS
                     ERSFESDLIVWRSNYSVKNSRLTDAYAGLKNQTAKIADFLSKKGIESKDITFSAVDIT
                     KDYQYIYNQNGTSQSIFDGYTLTQTVSISSTQVTKIETISREITELINQGIEINSMPP
                     QYYYTKLADLKIAMLAEATEDGKLRATTIAENGGARLGNLKNSTMGVFQIVALNSSEE
                     YSWGGSFNTSSKYKTATVTVRLQYNIK"
BASE COUNT         3975 a         2009 c         2298 g         4196 t
ORIGIN      
        1 aaacatgggt gcctatgggg attgtagtaa ttggaggtct ggttgtttct accttagtaa
       61 ctcttatcgt agttccaaca ttgtatgctg tgatgtctcg tagaggagaa cgcaataaag
      121 aagaaaagtt acgtaaatca tttacgttct ttgataaacc agtgagtaaa caatagttaa
      181 aattaccaga aaataaaata caccatatga agtcagtatt tattgtgttt aatcaagcta
      241 atacagaacg tgtagaatat atgcttgata gattatccat tagaggcttt accttttggg
      301 aaaatgtgca aggagtaggc agtatgaatg gagaaccgcg tcgtggtaca catacttggc
      361 cagaaatgaa ttctgcagtt atgacagtca ttcctgacga aaaagtagat gatttattga
      421 ttgctgttaa aaaactagat caacgtaata aagaagtagg cgttagagca tttgtttgga
      481 atattgagca aatggtatag ttacttaggt ttcgttagta atcttttcaa tatacttatg
      541 catcagtcga gatataggat agtcttctat gtctcgacta tttattttat ataatgattg
      601 tagtagtgaa ttgtcagttt ctatgtgtat acggataaaa atagtacgta taatttgatg
      661 acttagaaca tgttttattt cacttgagat actgtgaata gatgttttct cacatttttc
      721 tactatttct tgaaaatctt cactattgat aacgttttct atagatgttt ccttatcaca
      781 ttctactagt ggatattcaa ataaattatg ccaaatatca ttttgtgttc ttttttgaag
      841 atacgtatat tcaccattac gtatatccaa gtaaaaaaaa taacgtgttt tcgttgtagt
      901 ttttttctct tttttgggta aagaacttac gtgttgatgg gtatatgcat aacacataac
      961 ttgcaatgga caagttgaac acaatggacg agcaggaaca cattgcaacg ctccaaactc
     1021 cataatagcc tggttataag tgcttgcttg atgtgtgggg agtaaagatt gtgctaattt
     1081 cttaaattct cttttgccct cgttgctatt aatagctaaa aagataccga ataccctgct
     1141 taataccctg tacacatttc catctactac agcatgtgcc tcgttggctg caaaagagct
     1201 aatagctgcc gcggtatatt cgccaatgcc ttgtaaggta agtaagtggg ggtaatgagt
     1261 agggaatata ccatcataca actccacaac ctgctgtgcg gctttgtgca tattacgagc
     1321 tctcgaataa tatcctaacc cttgccaata cttcaaaacc tcttcttcat cagcttgtgc
     1381 taaggcaata acagttggaa accgttcgat aaaacgtata aaataagcaa gaccttgcac
     1441 taccctcgtt tgttgtaaaa taacttctga tacccaaata gtgtaagcat ctttggtatt
     1501 acgccaaggt aaattccgtt tatttacctg gtaccaatct attattattt cactaaaagt
     1561 caatgtcgta tattatatgt agcaaaagta gctatttttt caaattaaat caaacattca
     1621 actttttaga ttattttttt ttgaataatg tagattattg aaactaaaag tattatattt
     1681 gcagtccgtt tttaaaagaa aagtataact ataaaataga ctaaaatgac taaagcagaa
     1741 atcgttaacg aaatcgcaaa caaatcaggt attgataagg ttaccgtgtt gagagtagtt
     1801 gaatcattta tggatgaagt gaaggattcg ttagcttcta acgaaaatgt gtatttaaga
     1861 ggatttggta gctttgttgt aaaacaaaga gcagagaaag tggctcgtaa tatttctcgt
     1921 aatactacca ttactatacc agcacattca atcccatcat ttaagcctgc taaaactttt
     1981 gttgaggctg taaaaaataa aattatttaa ttgttataaa agaatacgga tatgccaagc
     2041 ggaaaaaaaa gaaaaagaca taaaatgtca actcacaagc gtaaaaaaag actaagaaaa
     2101 aatagacata aaaagaaaaa ataatttttt tgtctttcta aaacaactaa gcacaataag
     2161 aacaaactta aattatctgt tttgagtttg ttctttctgc ataccaatac atcacattta
     2221 tatactatat gaataacact ttacttcttg atgttcaatc taaagatatt tctatcgcat
     2281 tgatggaaaa taatactttg gtagaattta gcaaggagcc acgtgatatg tctttttccg
     2341 taggagatat ttttttagca aaagttaaaa aaattatgcc tggattaaat gccacgttcg
     2401 tagatgtagg atatgataaa gatgcatttc tgcactattt agatttaggt agtaatttcc
     2461 attctattgc acagtttacc aaaacactgg tcaatgacag gaaaaaaata ccttcaatgc
     2521 aaaaaaattc tggtttacct gacttagaga aagatggctt tattgctgat attttaaaat
     2581 ctggtcagga aattttggtg caaataacca aagaacccat atcaactaag ggtcctcgac
     2641 taacagccga aatatctata gctggacgat cattagtatt attgcctttt tcaaataaga
     2701 tttccatttc tcaaaaaatt aaatcggaag aagaacgcaa tcgacttcgt caattattag
     2761 ttagcataaa acccaaaaat ttcggtgtta ttattcgcac tgttgctgaa ggcaaaaaag
     2821 tggcagaatt agatgctgaa ctgaagattt tagaaaaaag atgggaagaa gctattgaaa
     2881 atattcagaa agccaaacaa tttccatcct taatcctcaa agaaatgagt agggctgaaa
     2941 ctattttacg tgatagtttt aatccgaatt ttcaacacat ttatgtgaat gagaaaacta
     3001 ttttcgaaga aatacaagct tatgtaggtt tgatagctcc cgaaaaaaat gatattgtaa
     3061 agctatatac cgatgatatt ccaatttttg atgctttcaa tatcaccaag caaattaaat
     3121 cctcattcgg taagattact cctttcaaaa acggtgccta tttggttatt gagcatacag
     3181 aggcattgca tgtaattgat gtaaatagtg ggaatcgatc taaagctggt aatgttcagg
     3241 aaaccaatgc gttagaggtt aatttagctg ctgttgatga ggtagcaagg caattacgat
     3301 tacgtgatat gggaggtatt attgttattg acttgattga tatgcaagaa aacgagcata
     3361 agcaacaagt gtacgaacgc atgaaggagc ggatgcaaaa cgattctgca aaacataata
     3421 tattaccatt aagcaagttt ggtttaatgc aaattacacg ccaacgtgtt cgaccacaga
     3481 cgcttataga tacgaaagaa acctgtccaa cgtgtgatgg aaatggtaaa atccgttcct
     3541 ccctgttttt tgtggatatg ttggagacaa aattaaatga aattaagaat actatgaaga
     3601 tacgaaaatg tattcttttt gtacatcctt ttgtgtatag ttttattaat gcgggtatca
     3661 tgagtattaa gaataaatgg atactcaagt ttggcttagg ttttaaagtg ttacccaatc
     3721 aaagctatag ttatttgcaa tatggctttt ttgatacaga tggaaatgaa attgacgtaa
     3781 aagatacggc tgatatggct taaatttttt gtgtatgtta cttaaagagc gttataaggg
     3841 tgttattgag tggtttcaac aacatatgcc tgtcgctgaa acagaactcc actatacaaa
     3901 ttcatttgaa ttattagtag ctattgtttt gtctgctcaa tgtacagata aacaggtaaa
     3961 tttaacaact cctgctttat ttaagtcttt tcctactcca caagctatgg ctaaagcttc
     4021 ttttgaagag gtttttgctc ttatcaaaag tatttcatat ccaaataaca aagcaaaaca
     4081 tctaatagct ttatctcagc aattagtaga taaatacgat ggagttatac cagacgatgc
     4141 cgagcgtttg caagagttgc ctggtgtggg tcgcaaaaca gccaatgtga tgcttgtagt
     4201 agcttttaag aagccagcta tgcctgtaga tacacatgta tttagggtag caaatagaat
     4261 tggtttaact actgcaaaaa acccattgca aagtgaaaag actttagtaa agaatattcc
     4321 caaccactta ttacctatag cacatcattg gcttatcttg catggtcgtt atacctgttt
     4381 ggcacgcaaa cccaaatgcg acgaatgtgg gatacgtact ttttgtaagt tttttaacaa
     4441 aaagtaaaaa ggtttctttc ggatttttta tatctttgta ggcatcatac ttttatgcct
     4501 gcaatctatg agtttgaata tagaaaatat cctgttaatt gggtcgttat tgctcttagt
     4561 aagtattatt gcaggtaaaa catcgtatcg ctttggtgta ccaacgttaa ttttattttt
     4621 agctgtaggg atgttgtctg gttctgaggg attaggaggg atacgttttg atgaccctca
     4681 gattgctcaa tttattggca ttgtttcatt aaactttatt cttttttctg gcgggttaga
     4741 tacccattgg catcaggtta agcctgtttt gtggcaaggt atcacgcttt cgactgtagg
     4801 agttttaatt acagccatat cgttgggtac atttgtttgg ctaattactg atttttcgtt
     4861 agtggaaagt ttgctgttgg ggtctattgt gtcatcaact gatgcagctg ctgtattttc
     4921 aattttgagg tcaaaaagtt tatctcttaa aaagaattta cgtcctactc ttgagcttga
     4981 aagtggaagt aacgacccta tggcttatgt tttaaccatt gcttttttaa ccttggttgt
     5041 tcatcaagat atgtcagttg tttctattat tcctctgttt ttacagcaaa tggtttttgg
     5101 tgctattttg gggtatgttt tcggacgaat aagcaaactt attattaatc gcataaaatt
     5161 agattttgaa ggcttatatc cagtgttagt cattgccctg atgtttttaa cgttttcggc
     5221 aactaatttt gttggtggaa atggtttcct tgcaatatac atatgtgccg tttatttagg
     5281 caaccaaaac ttaatacata agcgaactat cttaaaagtt tatgatggat tggcgtggtt
     5341 gatgcaaatt gtgcttttct taacattagg actattagtt tttccaagtc atattttacc
     5401 tgttgttggt attggattgt tggtatcagt atttttaata atcatagctc gtcctttaag
     5461 tgtttttctt agcttgcttc cttttagaat gcccatacgt agccgttttt atatttcttg
     5521 ggttggctta cgaggtgccg tgcctattgt gtttgctaca tatcccttac tggcaggtat
     5581 tgataaagca gatatgattt ttaatattgt cttttttgta tcggtctctt ctgtattact
     5641 acaagggact acattgggaa tagtcgcaaa atggttgggg gtagctgaca aaaaaaagga
     5701 aaccacatca tcttcatctg aggttttcaa agcagagtat ccaactgctt cgatgcaaga
     5761 aattattatt gctcccaatg cgtatgctgt taataaaaca attgctgaat tgtcttttcc
     5821 caaaagtgcg aatatagcca tgattcatcg tgatggaaat tatttaacac ctactgggtc
     5881 aacagtactt gctgctaatg atatattggt cgtattgtcg aataatgaag aaggcttgag
     5941 caaagcccaa gcctgtttgt cagcaacatc ggtataaggt cttgttatgc cctagtaaat
     6001 tctaacgctt gccctttggt atcctcgtta aattctccat tgctatatac tacgttccca
     6061 ttgataatgg tgtaggttac cttagaggag aatttttttc cttcgtaggg cgaccaccca
     6121 catttgctta aaatagtatc cgtgtttacc tgccaactag cattgcgttg cacaataaca
     6181 atgtcggcat aatacccttt gcgaatatag cctcgtttgt ttatttgata gagtttggct
     6241 gggttatgac acatcttttc taccacattt tcgatggtag ttttgccatg tttagctaat
     6301 tcgagcatac ttactagtgc gtgttgtatt tggggcatgc ctgatgtagc tgtaagagca
     6361 tctccttctt tttcggataa taaatgaggg gcatggtcag tgccaatggt atcgattttt
     6421 tgtgtattaa tagcagccag taaagcttct ttgtcttgag tagatttgat agatgggtta
     6481 catttaatac gagcacccaa ggtatcataa tcgttctcgt cgaatagcag ataatgagga
     6541 caagcttcag ccgttacatt ttttttatct aaacttacat tatcaaataa tgccaattcc
     6601 ttggcagtgc taagatgcaa aatgtgtaaa cgagtaccat atttttttgc caattcaatg
     6661 gcaagagctg tgcttttgta acatgcctca gctgaacgaa tctttgcatg gtacttcatt
     6721 ggaatcggat tttctccata ttcagcttta taagtggcag catttgcttt aacggttcct
     6781 tcgtcttcac tatgaatagc tactgggatt ttcacatctt cgaagatgcg atgcaataat
     6841 gaatctttat ctactagcat atttccagtt gatgaaccca taaataattt tatgccacac
     6901 actttggagg ggtcaatctt ttgtaattct tcgaaattgg aattagtagc acctaagtaa
     6961 aaagagtagt ttaccaatga tttagtagat gctaattggc atttttcttc aaaggcttgt
     7021 acggtaattg tttgaggatt agtgtttggc atgtccataa tagatgtaac accacccgcc
     7081 gctgcagctt tactctcggt aagtaaatct cctttgtgag taagtccagg gtctctaaaa
     7141 tgcacatgac tatcaatcac tccaggtaat acccagcagt catctgcatc aattttgtgg
     7201 actttttcaa ctatactttt tggaacttcg tctttataaa tggcaccaat tttgccatct
     7261 tttataagaa tagatgcttt gtaaattttt ccttcgttta caatgcgacc gttgctgata
     7321 agtaacataa tgtcaatata aaatgttcta tttgtgggtg attatttttt ttgtggaaat
     7381 tttctaaaga agctttccca ccgtagttgt aatactccga ttacagcctc tctaaatatt
     7441 ttaccactca tttttgatgt tcctaattcg cgattgataa aaatgacaga tacttctttt
     7501 atattgaatc cgcatttaaa agcagtaaat ttcatttcaa tttgaaaagc atatccaaca
     7561 aattttattt tatccaattc aatagtttcg agcacagctc ttttgtaaca tttaaagcct
     7621 gctgtagtat ctgcaatggc taagccggta acaaaacgta catattttga tgcaaaatac
     7681 gacatcaata cacggcccat gggccagttt actacattta ctccagttac gtatcgagaa
     7741 cctatcgcta catctgcacc tccttgagag caggcttcgt acagtcgtaa taaatcgtta
     7801 ggattatgag agaaatctgc atccatttca tatatatact cgtaagcaga agacaaagcc
     7861 cagttaaatc cagcaatata tgctctacct aaaccatttt tggcttttcg ctcaagtaaa
     7921 tgtaattgag tagagaactc tacttgtaac cgttttacaa tatctgccgt accatcagga
     7981 gagttgtcat caatgactaa aatgtgaaac gattttggta aagcaaaaac tgcacgaata
     8041 atattctcaa tattttcttt ttcgttgtag gttgggataa gaacaaggct gtccgacatg
     8101 atagtattta ctgaatttga gtggttatta atttgttccc aaagatacat aaaggattgt
     8161 aaataaattg agattttgtc ttcatttttt aatctttaac ctaaaattgt gtacttttgc
     8221 aaaaattttt cagtggatat taaagaattt tcgcaatcac tcatctcaca tcgggttgta
     8281 aaaggcataa atcagtgggt tgagtcttca cacaaaaacg tatgtataca aggtttttgt
     8341 ggatcttcgt gtgctctttt ttttgcatct tcaatattag ccaaaacaaa tacggtatat
     8401 atcctaaatg atgctgagga ggctgcgtat ttttatcacg acttattgca attattatcc
     8461 cctgatgatg tgtattattt tccttcatcg tacaaaaaac gagtgcgtga aggagaccaa
     8521 aaagatgctg ctaatcaagt gttgcgtact gaagctttaa acgatatttc tcaaaaacag
     8581 tcttggatac ttgttactta tcctgaagct attgctgaaa gagtaattcc tcaacaagat
     8641 ttgtcaaaag agacgcttca tttggctgtg ggagaaaaat tggatagcga ttttgttatt
     8701 tcattgcttg ttgaatatgg ttttgaacgc gtagattttg tatatgaacc agggcaattt
     8761 gctgtgcgtg gtagtattat agatgtgttt tctttttcac atgatgtccc ttttagaatt
     8821 gatttttttg gagatactat tgatactatt cgtacctttg atattgaaag tcaattgtca
     8881 atcgaaaaga aaacgcatat ctcaatcgta tcaaatagcg taaatataga agaaaatgcc
     8941 ttagttcctt tattcaaatt tttcacgaat aaggtcgtct atactataaa aaacacccca
     9001 tacgtagtag acttaattga taagataaac caagaaaatg acgaaacccg atttatttta
     9061 tctacagaat ggattgaaca atggcaaggg aataaagtaa ttgagtttgg aaattcacct
     9121 ttcttttctt ctcctcacat tattacactc aatcagaata ttcaaccaat atttcacaaa
     9181 aattttgact tacttgctgg tagtctgacg gagtatatag cacaacagta tcaaatctat
     9241 gtattgagtg ataatcccaa acaaacagaa cgattaaaag atatttttga agataaaaat
     9301 tttccaatca cgtttacacc catcaataaa accattcacg aaggatttgt agataatgat
     9361 ttgaagattt gcgtttttac agatcatcaa atttttgata gataccataa atattctctt
     9421 aaatcgcaaa aaacaaaagc aggaaaattg gttttatcgc tcaaagaatt aatgcagttt
     9481 caagttggcg attatgttgt tcatagtgag catggtgtgg gtaggtttgg tgggcttatt
     9541 cgtacagaaa ttaatggaaa aacacaagaa gttatcaaac ttttgtataa agatgatgac
     9601 attatttttg taaatatcca ttcattgcat cgtatttcca aatacaaaag caaagaagga
     9661 gagcctccaa agataaataa attaggtaca acagcttggg ttaatctaaa agagaaaaca
     9721 aagaaaaagg taaaagatat tgctcgtgat cttattgctt tgtatgcgaa acgcaaaagc
     9781 gaaaagggtt ttgccttttc tcctgatagt tatttgcaaa acgagcttga agcatctttt
     9841 ttatacgaag atacacctga tcagctcaag acgactattg ccttgaaaaa agacatggaa
     9901 agcgacaaac caatggatag gcttatttgt ggtgatgtag gttttggaaa aacagaatta
     9961 gcgattagag ctgcggctaa agcagtagcc gatagcaaac aagttgccgt gttggtgccc
    10021 actactgtat tggcatttca gcattttaat acctttaaaa aacgcttaaa aaagtttccg
    10081 tgtactgttg agtatattag tagagcccga aaaacatcag atgtaaaatt aattattgaa
    10141 aaaacaaaaa ctggtgcagt cgatattctt attggaactc ataaattgtt gagtaaagag
    10201 ttagaattta aagatttagg attgcttata attgatgaag aacagaaatt tggtgtttcg
    10261 acaaaagaaa aactgcgtca attacgctct catcttgata cgcttactct tacggcaacc
    10321 cctattcctc gtactttaca attttccttg atgggcgctc gtgatttgtc agtgattaca
    10381 actccaccgc ccaatcgcta tcctgtacaa acagagctta ttcgttttga cgatgatatt
    10441 atccgcgaag ccattgaatt tgagttagca cgcaacggac aagtattttt tgtgaacaat
    10501 agggtatcca atctgcctga attggaaatg caacttaaac gcttggttcc acatgctcgt
    10561 atagctgtag gtcatggaca gatggattcg gagaaactag aaagagtttt gttagatttt
    10621 attcatcacg aatatgatat tttattagca actaccatta tcgaatctgg tattgatatc
    10681 ccaaatgtaa ataccatgtt tattaataat gcccagtatt ttgggttgag tgatttgcac
    10741 caattacgag gcagggtagg tagaacgaat aaaaaagcgt tttgttacct aatttcacct
    10801 tctttagcca tattgcctac agatgctcgc agacgcttgc aggcaattga aaccttggca
    10861 gatttaggta gtggcattca tattgccatg caagatttgg atattcgtgg agctggaaat
    10921 attttgggag cagaacagag tggttttata acagacttag ggtatgaaac ataccatcgc
    10981 atattagatg aagcagtgaa tgagttaaag cacgaagagt ttgctgatgt gtttgcagaa
    11041 gaaatagaac atcacatgaa ttatgtggct gattgtatgt ttgaatctga tcttgagttg
    11101 ttgtttcctt cggattatgt gcagaatata tctgaacgta tggatttgta tcgccgatta
    11161 gatacgataa acaacgaaga agatttgcaa cattttgaga aagaattgat cgaccgtttt
    11221 ggtccgttgc catttgtctc tattgaattg atacaagtag tgcgtttacg ttggttgtgt
    11281 gctgcattgg gtattgagaa aattcaaatg aagcacggta gattgactgc ttattttatc
    11341 tctaatccat cttcccccta ttatcaatca gaaacttttg gtgctatgtt gttatttgct
    11401 caaacgcatc cacgcaatac ccagtttaga gaaataaacg ggaaacgctc tctgttgctg
    11461 tctggtatag atacgataca aaaagcatat actattttta aagatattcg agaataaagt
    11521 aaagtctatt gttgcctttt ttacattatc ccttttaaaa ataaacataa atgtatcttt
    11581 actcaaaata ttacctttat atttgtaata aaaagtaaaa tccttaaatt atgagtagta
    11641 ataaatcaat tatttcagta agtattgttg ctgtaagcat tattttatcc gtgtttattt
    11701 tatccaacgc gtttgttaat cgcaataaaa cagataatac aatatctgta acgggtttat
    11761 ctgaacgtag ttttgaatca gacttaatcg tttggcgaag taactattcc gtgaaaaatt
    11821 ctcgtttaac ggatgcttat gcagggttga aaaatcaaac agctaaaata gctgattttt
    11881 tatccaaaaa aggaattgaa agtaaggata ttactttttc agcggttgat attactaaag
    11941 attatcaata tatatacaat caaaatggca catcacaatc tatttttgat ggttatactc
    12001 ttacacaaac agtaagtatt tcttctactc aggttacaaa aattgaaact atttctcgcg
    12061 aaattaccga attaatcaat caagggattg aaattaactc aatgcctcct caatattatt
    12121 atacaaaatt ggccgattta aaaattgcta tgttggctga agcaacagaa gatggaaaac
    12181 ttcgtgctac aactattgct gagaatggtg gtgctcgttt gggtaattta aagaattcta
    12241 ctatgggcgt atttcaaatt gtggcattaa attcctcaga ggaatatagc tggggcggat
    12301 cgtttaatac ctcatcaaaa tataaaacag ccactgtgac ggttaggttg caatacaata
    12361 ttaaataaaa cacttttaca aacaaagata actaaacgaa gataattgtt tgtttagtta
    12421 tcatttgggc ttaattcttc tggatttaca ggctgaaaag tttcatctgt aagcgtat
//
LOCUS       DAOSMY010000004        10827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_6068_length_10827_cov_4.031656, whole genome shotgun sequence.
ACCESSION   DAOSMY010000004 DAOSMY010000000
VERSION     DAOSMY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6068_length_10827_cov_4.031656"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLD76_00235"
     CDS             complement(<1..624)
                     /locus_tag="PLD76_00235"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOU26468.1"
                     /translation="MENENKQGKQPNNNKPKGKFIVNMYWFYGIIAAVLIALNIFMNS
                     DASKEVSYSEFRNYAEKGYVESIVVITNKNAAEVVIKQDSSILNTVFGDDKGKVNSLI
                     VTIPSPEKMEEFVADNDKNLNIKTDIKYEERKNYLDTLLWSFGPFILIIIIWIFIMRR
                     MSGGGAGGNVFNVGKSKAQLFDKGANTMSVTFKDIAGLAEAKQEIQEI"
     gene            complement(662..1018)
                     /gene="rsfS"
                     /locus_tag="PLD76_00240"
     CDS             complement(662..1018)
                     /gene="rsfS"
                     /locus_tag="PLD76_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU26469.1"
                     /translation="MTDTQQLVNSIIAGIQEKKGSKIVVADLSKLSNSICSYFIICEG
                     GSNTQVDAIASSLKDYVKKETKERPLGMEGLENCEWVVMDYGDVMVHIMQREPRKFYD
                     LENLWQDAKLTEIADI"
     gene            complement(1086..2147)
                     /locus_tag="PLD76_00245"
     CDS             complement(1086..2147)
                     /locus_tag="PLD76_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU26470.1"
                     /translation="MAIYPQLIIDALRKVRYPGNNKDIVTNEMLEDDIRIEGNKVSFS
                     LILKANDPFAKSLVKAAEQAILTYVGEEIDIKGNISIKALPAPNPMLNPRSLTHIKHK
                     IAISSAKGGVGKSTVSSNLAVALANKGYKVGLLDADIYGPSIPKMFDIEEERPTVEVI
                     DGKDWIVPVEKMGIKILSIAFFIEKDNAIIWRGAMASNALKQLINDALWGDLDFFLID
                     LPPGTGDVHLTLLQTIDLNGAIIVSTPQQVALLDTVKGINMFLNEKINIPIVGLIENM
                     SWFTPAELPNNKYYIFGKEGMKNLAENMKLPFLGQIPIVQSICESGDSGSPIIINENS
                     IVGTSFDTITENILRNLKK"
     gene            complement(2172..2915)
                     /gene="trmB"
                     /locus_tag="PLD76_00250"
     CDS             complement(2172..2915)
                     /gene="trmB"
                     /locus_tag="PLD76_00250"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU26471.1"
                     /translation="MGKNKLKKFEDMDSYPHVFQFPFSKIQEQGFHLKNHWHTNFFQN
                     NHPIVLELGCGKGEYTVELAKLFPEKNFIGIDIKGARMWTGAKASLEQKMNNVAFIRT
                     NIELITHFFGTNEVSEIWITFPDPQMKKASKRLTSTRFMALYNEILQPNGLIHLKTDS
                     PFLYTYTQLMIEKNALDIHFQTDDLYHSNWVNEILSIQTFYEKQWIERGLSIKYIQFK
                     INKNTPLLEPEEEIEHDSYRSFNRSKRSM"
     gene            complement(2917..3405)
                     /locus_tag="PLD76_00255"
     CDS             complement(2917..3405)
                     /locus_tag="PLD76_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOU26472.1"
                     /translation="MKQILHHSNNLLIALSVGLLFSLQSCGEKSVYNEYNKIATVGWD
                     FGTPVRFETEINDTLSAYDITIQVRNNTDYAYQNFWIFVESTSPSGIVAKDTLEFLLA
                     DNTGKWLGKGISSTKEISGLYLENIKFPELGTYSFSLYQGMRSASLKGIESVGLRIVK
                     RQ"
     gene            complement(3425..4531)
                     /gene="ricT"
                     /locus_tag="PLD76_00260"
                     /pseudo
     CDS             complement(3425..4531)
                     /gene="ricT"
                     /locus_tag="PLD76_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(4544..5674)
                     /locus_tag="PLD76_00265"
     CDS             complement(4544..5674)
                     /locus_tag="PLD76_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU26473.1"
                     /translation="MQFSQIIGQDIIKQQLITSVREGRIPHAQLFLGQEGVGKLALAI
                     AYAQYVSCLNKGEHDSCGTCSSCIKYEKLIHPDLHMVFPFAKDGKTKEICDDLLPEWR
                     EFVTKNVYFSLEQWMDNIGAGNKQATIYANESNEILRKLQLTTYESAYKIMIIWLPEK
                     MNITCANKLLKILEEPPTKTLFILVSEQVEKIISTIISRTQMIKIPLIETHTMSSYLQ
                     AKHSISKENSINIARIANGNYIKALSIIKATEESQLNFTRYQEIMRYAYKNDVLATKK
                     WAEEMTSSSMGREKQKSFLDYAQHITRECFIKNIEINELNYLIDYEENFSSGFARFVN
                     EKNIQALMDFFADAATDIEQNVQGKFVFFDLGLKLMTQIKQN"
     gene            5945..7147
                     /locus_tag="PLD76_00270"
     CDS             5945..7147
                     /locus_tag="PLD76_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU26474.1"
                     /translation="MHTTKQITNNIYYVGVNDRTKHLFESLWEIPAGVSYNSYLIVDE
                     KVALVDTVDACYSDLFLSKIEGVLGEKPIDYLIINHMEPDHSASIATIKRKYPSIQIV
                     GNAKTFGMLDGFFNIKDNLYEIKEADTLTLGKTVLRFYLTPMVHWVETMMTFDETNKV
                     LFSGDAFGCFGALDGGVLDSQINVDKFWPEMERYYSNIVGKYGYPVQRALAKLSNLDI
                     QTICSTHGPVWQKYLQKTISIYNDLSLYQGKKGVVVMYGSMYGNTEQMAEVIADTLVA
                     NGIENVVMHNVSKSTPSYIIADIFRYQGLIIGSPTYSNELFPLVESMLSKIKNRDIKN
                     RYFAYFGSFSWAGVANKKLAAFAEQVGFEVVYEGGVEQKFALKHDKYVECVKLAESMA
                     QRLNNSSL"
     gene            complement(7229..7675)
                     /locus_tag="PLD76_00275"
     CDS             complement(7229..7675)
                     /locus_tag="PLD76_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU26475.1"
                     /translation="MSVNKVILVGNVGKDPEVRYLENNVAVAKFSLATTEKGYKMNNG
                     TVVPDRTEWHNIVMWRGLAEIAEKFVKKGSQLYIEGKIQSRTWDDKNGVKHYATEILC
                     ENMQMLSKKSEAESGNAPLNDKSVSTKAEETPFGNSDNIADDDLPF"
     gene            7820..8428
                     /gene="udk"
                     /locus_tag="PLD76_00280"
     CDS             7820..8428
                     /gene="udk"
                     /locus_tag="PLD76_00280"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOU26476.1"
                     /translation="MIIIGIAGGTGSGKSTVAKEILSQLPDGDIAFLPQDSYYKDSSH
                     LPMDKRIEINFDHPDSIEFDLLISHIKELKKGNSVQQPIYSYITCSRAEETVLIKPAK
                     IIVVEGILIFTDKELRKLMDLKVFVDADSDDRLIRVINRDIIERGRSVNSVIERYQKV
                     LKPMHLQFIEPTKRYADLIIPEGGHNKVAISILQHYIDNKLR"
     gene            8434..9171
                     /locus_tag="PLD76_00285"
     CDS             8434..9171
                     /locus_tag="PLD76_00285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU26477.1"
                     /translation="MQQTTLEKILFLDIETVPQEATIKKMPESLAHLWMDKLDTLKRR
                     MPDRYDDDSSIDDLYRKEAGIYAEFGKIVCISVGIIYLKDGEKHCRIKSFFGHDEKTL
                     LTEFSGMLLKFMQSAEYQLCGHNIKEFDVPYIARRMLINGLPLPDVLQVAGKKPWEVK
                     FLDTLELWRFGDYKNYTSLKLLTAIFNIPTPKDDIDGSEVATVYYEHGDINRIAIYCQ
                     KDVVATIQVYLKLIGEPIIQDKHIQLV"
     gene            9193..10575
                     /locus_tag="PLD76_00290"
     CDS             9193..10575
                     /locus_tag="PLD76_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU26478.1"
                     /translation="MKKCIYLLLFIFLSIICVSCISGENQGNQENIPDLEGILQRGKI
                     RVITLYGPLSYFNYKDNVMGYEYEFAQQLSKELGVEMEMIVAKNIANMIELLENGEGD
                     LIAYRVPRTLEYQQRVSFTDREYVTSQVVIQLQSDSILTDVLQLAGKEVYVPESSIYH
                     DRIVDLNNEIGGGIHIVAVNDSVSVDALIVQTAQQLIPMTIADADIARLNKTYFSSID
                     YSLSISFPQRYAWAVRNDNPELLAYINKWNAKNAKNMKLSSIYNKYFQRTKYFESEGF
                     EQKKLHQLSAFDDYFKKYASIVSWDWRLIAAIAYKESKFDPTIVSWAGAAGIMQLMPS
                     TARTMGFSEHDLHSPEQSIKAGVKYLQHLQGIFQQIPVKEERIKFVLAAYNSGPGHLF
                     DARALALKYGKDPNIWHDNVEIYVRLKSNPEYYTDEVCKHGYLRGDETANYVEFVENK
                     FEEYKTRIGK"
BASE COUNT         3342 a         1775 c         1816 g         3894 t
ORIGIN      
        1 aatttcttgt atttcttgtt tagcttcggc taatccagca atatctttaa acgttaccga
       61 cattgtgtta gctcctttgt cgaataattg tgcttttgat ttaccaacat taaacacgtt
      121 gcctccagca cctccaccac tcatccttct catgatgaaa atccaaatta taatgatgag
      181 tataaatgga ccaaacgacc aaagtaaggt gtcaagataa ttcttacgtt cttcgtattt
      241 tatgtctgtt tttatgttta aattcttgtc attatctgct acaaattctt ccattttttc
      301 gggagaagga attgttacaa ttaatgagtt tactttacct ttgtcatcgc caaaaacagt
      361 gtttaaaatt gatgagtctt gtttgattac tacttcagca gcattcttat tggtaattac
      421 aacaatcgat tctacgtatc ccttttcggc ataatttcta aattcagaat aagatacttc
      481 tttacttgca tccgaattca taaatatatt tagagcaatt agtacggctg caattatacc
      541 ataaaaccaa tacatattta caataaattt ccctttgggt ttattattgt ttggttgttt
      601 gccttgttta ttttcgtttt ccatgttttt tccaaataat cttaatcaat attgattttt
      661 tttatatatc agcaatttct gttagtttag catcttgcca taaattctct aaatcataaa
      721 atttacgcgg ttcgcgttgc ataatatgta ccattacgtc accataatcc attaccaccc
      781 attcgcaatt ttctaaacct tccatgccca atggtcgttc ttttgtttct ttttttacgt
      841 agtctttaag tgaacttgca atggcatcaa cttgggtatt tgatccccct tcgcaaatga
      901 taaaatagga acaaatgctg ttacttagtt ttgagaggtc agctactacg atttttgagc
      961 cttttttttc ttgtattccg gcaattatgc tgtttactaa ttgttgtgta tctgtcatat
     1021 ggttaatttt tctgcaaata tagttgtaaa gtatggataa tgaaatattt ctgttttaat
     1081 tacctctatt ttttgagatt acgtaagatg ttttctgtaa ttgtatcaaa acttgtgcca
     1141 acaatactat tttcgttaat aataatagga gaaccactat ctcctgattc gcaaatgctc
     1201 tgaacaatag gtatttgtcc aagaaaaggt aatttcatgt tttcagcaag atttttcatc
     1261 ccttcttttc caaaaatata atatttgtta tttggcagtt ctgctggtgt aaaccatgac
     1321 atattttcta ttaaacctac tatgggaata tttatttttt catttaaaaa catgtttatg
     1381 cctttcactg tatccaaaag ggctacttgt tgaggagtac ttacgataat ggctccgttt
     1441 aaatcgatgg tttgcaaaag cgtaaggtgt acatcgcctg ttcctggtgg taaatcgatt
     1501 aagaaaaaat ctaaatcgcc ccaaagggcg tcatttataa gttgtttcag ggcattgctc
     1561 gccattgctc cacgccatat aatggcatta tctttttcta taaaaaaggc aatagagagg
     1621 attttaatac ccattttttc tacggggaca atccaatctt taccatcaat aacttctaca
     1681 gttggtcttt cttcttcaat gtcaaacatt tttggaatgg atggtccata gatatctgca
     1741 tcaagcaatc ctactttgta tcctttatta gccaatgcta ctgctaagtt tgatgaaaca
     1801 gttgattttc caacacctcc tttggctgaa gagatggcaa ttttgtgttt aatatgggtt
     1861 aaacttcttg gatttaacat cgggttagga gcaggtagtg ctttgatact aatattccct
     1921 ttgatatcaa tttcttcgcc tacataggtt aatatagctt gttctgccgc ttttactaat
     1981 gattttgcaa acggatcatt tgctttcagt atcagagaaa atgacacttt atttccttca
     2041 atacgaatat catcttcaag catttcattc gtaacaatat ctttattatt accaggataa
     2101 cgcacttttc taagtgcatc aatgatgagt tgtggatata tagccatggt tttatctgtt
     2161 taattagtag attacatact tcgtttgctt ctattaaaac tcctgtaaga gtcgtgttct
     2221 atctcttctt cgggttctag taagggggta tttttattta ttttaaattg gatatatttg
     2281 atactcaatc ctctctctat ccattgtttt tcgtaaaaag tttggatgga aaggatttcg
     2341 ttaacccaat tagaatgata caaatcgtca gtctgaaaat gaatgtctaa tgcatttttc
     2401 tcaatcatta attgtgtata ggtatacagg aatggactgt ctgtttttag gtgaatcagc
     2461 ccgtttggct gtagaatctc gttatacagt gccataaagc gggtagatgt taatcgcttg
     2521 ctcgcttttt tcatttgcgg gtcgggaaag gttatccata tctctgacac ttcgtttgtg
     2581 ccaaaaaaat gtgtaatcaa ttcaatattt gtacgaataa aagcaacatt gttcatcttt
     2641 tgttcaagcg aagcttttgc tcccgtccac atacgtgctc ctttaatgtc aatgccaata
     2701 aagttttttt caggaaataa tttagccaat tctaccgtat attcgccttt gccacatccc
     2761 aattcaagta caattggatg attattctga aagaaattcg tatgccaatg attttttaag
     2821 tgaaatcctt gttcttgaat cttgctgaaa gggaactgaa acacatgtgg gtaagagtcc
     2881 atgtcctcaa attttttcaa cttatttttt cccatattat tgacgtttta ctatgcgtaa
     2941 tcctacgctt tctattcctt ttagtgatgc acttctcatt ccttggtaaa gagaaaaaga
     3001 ataggtgcca agctctggga atttaatatt ttccaaatat aagccagata tttctttcgt
     3061 agagcttatg ccttttccca accattttcc cgtattgtct gccaataaaa attccaatgt
     3121 atcttttgct acaataccag aagggctagt tgattcgaca aaaatccaaa agttttgata
     3181 cgcataatct gtgttattac gtacttgaat cgtaatgtca tatgctgaca gtgtatcgtt
     3241 gatttcagtt tcaaagcgaa caggggtgcc aaaatcccat ccaacagtag ctattttatt
     3301 gtactcatta tacactgatt tttccccaca actttgtaaa gaaaaaagca gccccaccga
     3361 taaggcaatt agcagattat tgctgtgatg aagtatttgt ttcattggta gatgcatttt
     3421 tatgttttct acgtttatta tttcgttttt ttgatttttt gttatcaaaa cgggttaaac
     3481 ttccttcttc taagtcagtt gcaaaacttt tagtttcttc ttcctctttc tttgtttctt
     3541 tgatattgcc ttgaatagca ggacgtactc cttttttatt tagagcaatt atttccttta
     3601 ctttatccag agataattca actacatttt ttggaaaatt tggagcttga gaatatgcca
     3661 tctttttttc aaaaatatct aatttaaaga aataataggt gcaatcttct ccttctagtt
     3721 gaatgtccct tgaaggaaat tcttttaaag cttctgtata ggtagaaagt tcgtaattta
     3781 agcaacattt tagtttagca cattgtcctg ccaatttttg tggatttaat gataaatctt
     3841 gtacacgtgc agcgtgtgtg ccaacggatg aaaaactatt taaaaaagta gaacaacaca
     3901 gctctctacc gcacggacct gtacctccaa tacgtcctgc ttcttgacga gctccaattt
     3961 gtttcatttc gatacgcact tgaaatgttt cagccaatac cttgataagt tgacgaaaat
     4021 caacgcgttg ttctgcaata taataaaata ttgctttgtt cccatctcct tgatattcca
     4081 catcgccaat tttcatatct agtttaagat cttgtgcaat ttgacgagat tttaacatgg
     4141 catcgtgttc ttttgcctta gattcttccc acttttccaa atcagtttgc tttgccttac
     4201 gataaacccg tcttacttca atggttttag ggtcaatgcc attctttcgc atttgtctgt
     4261 ctactaacaa gccaactagg gttactgttc ctatgtcgtg tccaggggtt gattctacag
     4321 caacaatatc tcctttttct agttttatat tggtcgaatt aagataatat ccttttctcg
     4381 tgtttttgaa ttgtacttct acaaaattac aatttatctg tgtttcgggt atatccgaaa
     4441 gccagtcgta aacactcagc atttgcagag agtttttttt gcacccgcac actttaatgc
     4501 cacaggttga tttcgctaat tcatattcca taagtttgtt tatttagttc tgtttgattt
     4561 gagtcattaa ttttaatccc aaatcaaaaa atacaaattt cccttgtacg ttttgctcaa
     4621 tatcggttgc tgcatctgca aaaaaatcca tcaatgcttg aatatttttt tcgtttacaa
     4681 atcgtgcaaa accagaagaa aaattttctt cataatcaat taaataattt agttcgttta
     4741 tttctatatt tttaataaaa cattcgcgcg taatgtgttg agcataatcg agaaaacttt
     4801 tttgtttttc cctacccata gaggacgatg tcatttcttc tgcccatttt tttgttgcta
     4861 gcacatcatt tttataagca tagcgcatta tttcttgata gcgagtaaag ttaagttgcg
     4921 attcttccgt tgctttgatg atcgaaaggg ctttaatata attgccattt gcaattcgag
     4981 caatattgat agaattttct ttcgaaatag agtgctttgc ctgtaagtac gaactcattg
     5041 tatgtgtctc aatcaacggt attttaatca tctgcgtacg agatataatg gtagaaataa
     5101 ttttttctac ttgttcactt accaaaataa ataatgtttt tgtcggaggt tcttcgagta
     5161 tttttagtaa tttattagca cacgtaatgt tcattttttc tggcaaccaa ataatcatta
     5221 ttttatatgc agactcgtat gtggttaatt ggagcttacg tagtatttca ttactttcgt
     5281 ttgcataaat agtagcttgt ttatttcctg ctccaatatt atccatccac tgttctaggc
     5341 taaaataaac atttttcgtt acaaattctc tccattcggg gagtaaatca tcgcaaattt
     5401 cttttgtttt accatctttt gcaaaaggga ataccatgtg taaatcagga tgaatgagtt
     5461 tttcatactt tatgcatgat gaacatgtgc cacaagaatc gtgttctccc ttatttaaac
     5521 aagacacata ttgggcatat gcaatagcta atgctagctt cccaactcct tcttgcccca
     5581 aaaataattg tgcatgaggt attctccctt cacgtacact cgtaataagt tgttgtttaa
     5641 ttatgtcttg tccaataatt tgcgaaaatt gcatcgaact atttttgttg ttataagatg
     5701 atctcgaaac ctatctttgt taatttattt actgtaaaaa aatagaatct tatcaagaga
     5761 taagaactaa cttacaaaga tatataaaat acatctcttt tacaatacac aagggttaaa
     5821 cactcttaaa tatcagaatg tcatataaaa aatgttatat cgtggctatc ttcaatttta
     5881 tcaagtagtt gtgtagcata ttttttttat ctttgcactt taatatacat ataaaaattt
     5941 tgttatgcat actactaaac aaattacaaa caatatttat tatgttggtg taaatgacag
     6001 aacaaagcat ttattcgaaa gtttatggga gattcccgca ggcgtatcat ataattcata
     6061 cctaattgta gacgaaaagg ttgcgttagt agatacggtg gatgcttgtt attctgattt
     6121 attcctgtca aaaatagagg gtgttttggg cgaaaaaccc attgactacc tcattattaa
     6181 tcacatggag ccagatcatt ctgcttctat tgcaacgatt aagagaaaat atccttccat
     6241 tcaaattgta gggaatgcca aaacatttgg catgttagat ggctttttta atattaaaga
     6301 taatttgtac gaaataaaag aagcagatac cttaacattg ggaaaaactg ttttgcgttt
     6361 ttaccttaca cccatggtgc attgggtaga aaccatgatg acgtttgatg aaacgaacaa
     6421 agtattgttt tctggagatg cttttggatg ttttggtgcg ttggacggag gggtgcttga
     6481 ttcgcaaatt aatgtggata aattttggcc agaaatggag cgatattact ccaatattgt
     6541 tggtaaatat ggttatccag tgcaacgtgc attagctaaa ctaagtaact tagatattca
     6601 aactatatgt agtacacatg gtcctgtttg gcaaaaatat ctacaaaaaa cgatatctat
     6661 ttataatgat ttaagtttgt atcaaggaaa aaaaggagtg gtggtgatgt atggaagtat
     6721 gtatggaaat acagagcaaa tggctgaagt tattgctgat actttagttg ccaatggtat
     6781 tgagaatgtg gtaatgcata atgtctcaaa atcaacgccc tcgtatatta ttgcagatat
     6841 ttttaggtat caaggcttga ttattggtag tcctacatac tcaaacgaac tattcccttt
     6901 ggtggaatca atgttgagta aaattaaaaa tagagatatt aaaaacagat attttgctta
     6961 ttttgggtcg ttttcgtggg caggtgttgc caataagaag ctcgctgcat ttgccgaaca
     7021 agttggtttt gaggtagtat acgaaggtgg agtagaacaa aaatttgcat taaagcacga
     7081 taaatacgtg gaatgcgtaa aattggctga aagtatggct caacgattga acaatagttc
     7141 attataaaat tgtctcaaaa agagttgata aaaaaagaca gaacaaataa ttgctctgtc
     7201 ttttttttta ggctagtgaa tgactgaatt aaaatggcaa atcgtcatct gcgatgttgt
     7261 cgctattgcc aaaaggggtt tcctctgctt tggtgcttac agatttgtcg tttaaaggag
     7321 catttccact ttctgcttct gattttttgc ttaacatttg catattttcg cacaaaattt
     7381 ctgttgcata atgttttaca ccattcttat catcccatgt tctactttga atttttcctt
     7441 caatgtataa ttgagagcct ttttttacaa atttttcagc aatttcagct aatcctctcc
     7501 acataacaat attatgccac tctgttctat caggcacaac ggtgccatta ttcatcttat
     7561 accctttttc tgttgttgct aatgaaaatt tagctactgc aacattgttc tctaaataac
     7621 gtacttcagg gtctttgccc acatttccga ccaaaattac tttgttaact gacatctttt
     7681 ttctagatta agtaaattta ttattgttaa ttaaatcggt gcaaagataa ataaaaaata
     7741 gttttttttg catctttttt tatctttctt aaaaatattt ttctacctta gtgcattctt
     7801 aatttaatat actgaaaata tgataattat aggaattgct ggcggaactg gttctggaaa
     7861 aagtactgta gcaaaggaaa tattgagtca attgcctgat ggcgacattg cttttttacc
     7921 acaagattct tattataagg atagtagtca tttgccaatg gataaacgca tcgaaataaa
     7981 ttttgatcat ccagattcta tcgaatttga tttgttaatt tcccatatca aggaattgaa
     8041 aaaaggaaat tctgttcagc aacctatcta ttcatatatt acttgctctc gtgctgaaga
     8101 aacagtactt ataaaacccg caaagattat tgttgtggaa ggaattctga tttttaccga
     8161 taaagaatta cgaaaactaa tggatttaaa agtatttgtt gacgcagatt ccgacgatag
     8221 gttaattagg gttattaata gagatattat agaaagagga cgttcggtta attctgtaat
     8281 agaacgttat caaaaagtat taaaaccaat gcatttacaa tttattgaac caactaaacg
     8341 atatgcagat ttaattattc cagagggagg acacaataaa gtagctatca gtattttaca
     8401 acattatata gacaacaaat taagataaat gatatgcaac aaacaacact tgaaaagata
     8461 ttatttttgg atatagaaac tgttccccaa gaggcaacta taaaaaagat gcctgagtcc
     8521 ttagctcatt tgtggatgga taaacttgat accctaaaac gtagaatgcc tgataggtat
     8581 gatgatgata gctctattga tgatttgtat agaaaagaag ctggtatata tgctgaattt
     8641 ggaaaaattg tttgcatttc tgtcggaatc atttacctaa aagatggcga aaaacattgt
     8701 cgtattaagt cattttttgg gcatgatgag aaaacgctct taaccgaatt ctcaggaatg
     8761 ttattaaaat ttatgcaatc ggctgaatat caattatgtg gtcataatat caaagagttt
     8821 gatgtgccat atattgctcg acgaatgtta attaatggct taccattgcc tgatgtattg
     8881 caagtggctg gaaaaaaacc gtgggaggtt aagtttcttg ataccttaga actatggcgt
     8941 tttggagatt ataagaatta tacctcatta aaacttttaa cagcaatttt taatattccc
     9001 actcccaaag acgatattga tggaagcgag gtagcaactg tgtattacga acatggagat
     9061 ataaatagaa tagcaatcta ttgtcagaaa gatgtcgtgg caacaattca agtatatcta
     9121 aaactcattg gagaacctat catccaagat aaacacatac aattggttta aatgtttata
     9181 aaataaagat gaatgaaaaa atgtatctat ttattgctat ttatttttct ttccataatt
     9241 tgcgtatcct gtattagtgg agaaaaccag ggaaatcaag aaaatattcc agatttagag
     9301 ggtattttgc aacgtggaaa aattcgtgtt attacgttat atggtcctct atcgtatttt
     9361 aactataagg ataatgtaat ggggtacgaa tatgagtttg cacaacaatt aagtaaagaa
     9421 ttgggtgtag aaatggagat gattgttgct aaaaatattg ccaacatgat agaactcttg
     9481 gaaaatggag agggcgattt aattgcttat cgtgttccac gaacattgga gtatcaacaa
     9541 cgtgtaagct ttacggatag agaatatgta acatctcagg ttgttataca attacaatca
     9601 gatagcatac ttactgatgt actacaatta gctggaaaag aagtatatgt gcctgaatca
     9661 tcaatctatc atgatagaat tgtcgatttg aataatgaaa tagggggagg tatacacatt
     9721 gtagcagtta atgattctgt atctgtagat gcccttatcg tacaaacagc acaacagctt
     9781 attccaatga caattgccga tgctgatatt gctagattga ataaaacata tttttcttca
     9841 attgattatt ctttatctat cagttttcca caacgttatg cttgggctgt gagaaatgat
     9901 aatcctgaat tgttagcata tatcaacaag tggaatgcta aaaatgctaa aaatatgaag
     9961 ttgtcgagta tttataataa gtattttcaa cgtaccaaat attttgagtc tgaaggattt
    10021 gaacaaaaaa aactacatca actttctgcc tttgatgatt attttaagaa atatgcatcc
    10081 attgtttctt gggactggcg tttaattgct gctattgcat acaaggagtc aaaattcgat
    10141 cctaccattg tatcttgggc gggtgctgct ggtattatgc aactgatgcc atcaactgca
    10201 cgtacaatgg gttttagtga acatgattta catagtccag agcaaagtat aaaagcggga
    10261 gttaagtatt tacaacattt acagggaatc ttccaacaaa ttcctgttaa agaagaaaga
    10321 ataaagtttg tattagctgc atataattct ggacctggtc atttatttga tgctcgggca
    10381 ttagctctta aatatggcaa agatcccaat atctggcacg ataatgtaga gatttatgta
    10441 aggttaaagt ccaacccaga atactatacc gatgaagtat gtaaacatgg ctatctcaga
    10501 ggagacgaaa cagctaacta tgtagaattt gttgaaaata aatttgaaga atataaaaca
    10561 cgtataggta aatgattgat atatcaagtt taatcttggt attgtttttg attcataagg
    10621 tcttgttatt tttgattatt cccatagatt ctataacaca taaaagaata tcaataaaaa
    10681 agcatcctat aacaaaatag gatgcttttt tatatgaagg agatatgatt gttagaaaga
    10741 aacttcttct gcttctttta gattcacatc tgttacactg cgtttagctt gcataatttt
    10801 ttcatattca tcacgcgaag ctactac
//
LOCUS       DAOSMY010000005        10731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_6135_length_10731_cov_3.624859, whole genome shotgun sequence.
ACCESSION   DAOSMY010000005 DAOSMY010000000
VERSION     DAOSMY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6135_length_10731_cov_3.624859"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="mnmA"
                     /locus_tag="PLD76_00295"
     CDS             <1..741
                     /gene="mnmA"
                     /locus_tag="PLD76_00295"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU26479.1"
                     /translation="GKDFDKIATGHYAQTLIKDDTYYLSTAVDKHKDQTYFLSRISYE
                     QLSKSLFPIGDLPKSRVREIASLQQLPSATRKDSQGICFLGKINYSDFIKRYVGVKKG
                     HIIEYETGKVVGQHNGVWFHTIGQRKGLGLSGGPWYVVAKQVDENIVYVSNGYDVVTQ
                     YGMMIPLQDLHIISGNPTIYDNPTRVAFKIRHTPEFTFGTIHRKGNTYCIESEEKIQG
                     IAPGQFGALYDEKCEICVGSGIIAEYKL"
     gene            complement(815..888)
                     /locus_tag="PLD76_00300"
     tRNA            complement(815..888)
                     /locus_tag="PLD76_00300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(852..854),aa:Arg,seq:tct)
     gene            complement(955..3267)
                     /locus_tag="PLD76_00305"
     CDS             complement(955..3267)
                     /locus_tag="PLD76_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU26480.1"
                     /translation="MSRVIRYISLLIIVLLSACSATKYVPENKYLLDNVRIKSDIKNF
                     KSTELKTFLRQTPNSKMFDLISFNLGIYNLSGNNTDVWINRFLKRIGNPPIIFDSTLT
                     ERSEVELQRFFKNKGFENAQVESSVVFNKRKAKVTYNIISNNPYVINTIEYRIQDPII
                     KDLIEKDSSSSLIRKGNLFDVDVLDNERVRITRLLRNNGYYYFNKEYVRFLADSSLNT
                     NSVDVIIRIRKMERTGERNTVLRSNHEVFSINSITYILVNDVLDINGYTNQKTKKDTL
                     FYKDKRIIYNNLFINPSILDDNTFITIGDNYSEYDTEKTYRRINNLHVSQFLRIDFSE
                     TPLLSDSIRKLDCTILLTPSNNQSFAIEAEGTNSAGDFGIAGKFGYTHRNAFTGGEKF
                     GLQLRASNEAIGSIRNILSYSAWEIGGETTLQFPRLMFPFVDRDFQRNSNANTEFNLN
                     YSYQTRPDFARTIAGIGLKYRWDSYRQSNMFHTLDFLDLSYVYLPPYSISDEFRNKYL
                     NSSTLLKYSYEDHLILRTGYSFSYTSKLLQQRRNGFSAKAGIETAGNSFYGIASLLKT
                     PKQENSYVIGNIKFSQYIKLDGEYSYTQFLDPSNRIVYHIAMGIGIPYGNSSILPFEK
                     RYYGGGANSVRGWQVRTLGPGSYNNNGITDYMSQSGDMRADFNIEYRTKLVWLLELGA
                     FIDAGNVWTLLPYGEQVGGNFDINNIYREIALGYGIGIRFDFSYFLLRLDWGIKAYDP
                     SKIGSDAWRFSSNWNIMKDTALHFAVGYPF"
     gene            3361..4107
                     /locus_tag="PLD76_00310"
     CDS             3361..4107
                     /locus_tag="PLD76_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU26481.1"
                     /translation="MLSKSKIKFIRSLRSKKQRNDLRLFIAEGQKTVDDLLLYFPCSM
                     LFHTSEYCTSHSVSYLQEVTPEELKQISFLEEPQQVLGVFVMPEPIWSNDYLEGELII
                     ALDMIQDPGNLGTILRVADWFGIKHIVCARGTVDVFNPKTIQATMGAIGRVHVYIVDM
                     GEFLQQLPHKIPVYGTFLEGENIYSTNVQNEAVIIMGNEGNGISQAIEKHVTHRITIP
                     SFPPNQSTSESLNVAMATAIVCAEFRRRLF"
     gene            complement(4088..4393)
                     /locus_tag="PLD76_00315"
     CDS             complement(4088..4393)
                     /locus_tag="PLD76_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26482.1"
                     /translation="MIVLNVSLCVENILPLNHETPSTQFYNGIIFSLQNTPKDSIEDI
                     IQWQTNNSGKTIYDYLSNMGAQPIKIGTHLSELYVLLDIDFVSSIVTAHSSIRKVVS"
     gene            complement(4464..5231)
                     /locus_tag="PLD76_00320"
     CDS             complement(4464..5231)
                     /locus_tag="PLD76_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOU26483.1"
                     /translation="MKSLIFVLSLFFVTSTFLSCTKEERVPSSVISEEKMVDILVDVH
                     MVEAMARHNQLKDNNQRANMYYNYILEKHNITLNQLDSSISWYAKHPTTYIRVYDQII
                     PRLEHSLEMVRTNANTGGQALRTLDIWEKKNEYQLTSLQNSNVPFRIEENSTIRFSLG
                     DEFVLKTKFSCKNVPSSLQLVMQVKIHFTDSTESIFEKKIVTTNPDFEQILRFQPDSK
                     KQIAMISGDLCYLIDKIDNPIPIEIRIFNVEMEQITK"
     gene            complement(5244..5867)
                     /locus_tag="PLD76_00325"
     CDS             complement(5244..5867)
                     /locus_tag="PLD76_00325"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOU26484.1"
                     /translation="MKKNKLTISIISITIILTILLLDQIVKIWIKTHFAIGDEIKITN
                     FFYLHFIENEGMAFGMSFFDKKYLTLFRIVASIGIAYYIYKSIKNNSEQLFIISLSLI
                     FAGAIGNIIDSLFYGQIFTESTLFSNATWTPWGQGYAPIFYGKVVDMLYFPLISGTYW
                     SWIPIVGGTSFTFFAPVFNIADASITIGVILILVYEIFFSPSSKKIQ"
     gene            complement(6054..6434)
                     /locus_tag="PLD76_00330"
     CDS             complement(6054..6434)
                     /locus_tag="PLD76_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOU26485.1"
                     /translation="MGEKTRYSDSELEEFRAIIKDKLEKAFVDYEQLKAALNNLDGND
                     TTDTSPTFKVLEEGATTMSKEEAGRLAQRQMKFIQHLQAALVRIENKTYGICRETGAL
                     IPKERLRAVPHATLSIEAKQSGKK"
     gene            complement(6528..9959)
                     /gene="ileS"
                     /locus_tag="PLD76_00335"
     CDS             complement(6528..9959)
                     /gene="ileS"
                     /locus_tag="PLD76_00335"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOU26486.1"
                     /translation="MNTKFSEYTHLDLSAINKDILAYWDKHDIFKKSIDTRTGNPTFV
                     FYEGPPSANGMPGIHHVMARTIKDLFLRYKTMKGYQVQRKAGWDTHGLPVELGVEKSL
                     GITKEDIGKSISVEEYNAACRKDVMKYTKEWESLTHKMGYWVDMNHPYITYDNKFIES
                     IWWLLKELYNKNLLYKGYTIQPYSPAAGSGLSSHELNQPGCYKDVKDTTVVAQFEIDN
                     PIAELTQFGKAYFLAWTTTPWTLPSNTALCVGATITYVAVQTYNPYTFEPQTVILAKD
                     LVSAHFNGKASSLSLSEYTAGDKLIPYSIIAEYKGSELVGMTYKQLIPFFKPDEGAFS
                     VISGDFVTTEDGTGIVHIAPTFGADDARVAKMNNIPAMLLIDKNGNERPLVDLQGKFY
                     TINDIDNDFVAQRTNIALYSPFAGRYVKNAYDTSLNENDATLDIDLCVMLKQQGLAFK
                     IEKHIHNYPHCWRTDKPVLYYPLDSWFIRSTACRETMVELNNTINWKPQATGTGRFGK
                     WLENLQDWNLSRSRYWGTPLPIWRTEDKQEEICIGSIAELMAEIEKAIQAGMMNKNPF
                     KDFVVNDFSEDNYGISRIDLHRPYVDNIILVSPSGKPMKRETDLIDVWFDSGAMPYAQ
                     LHYPFENKELIDKQTNFPADFISEGVDQTRGWFFTLHAISTMIKGSVAFKNVVSNGLV
                     LDKNGNKMSKRLGNAVDPFSTIETYGSDALRWYMMTNASPWDNLKFDTEGIEEVKRKL
                     FGTLYNTYSFFVLYANVDGFAYKEPEVPMEQRQEIDRWIISLLHSLVKEVDENYADYE
                     PTRAGRAITTFVTDNLSNWYVRLNRRRYWAGEMTLDKLAAYQTLYTCLETVAKLMAPI
                     APFFAERLFLDLNSVTQKDISESIHLAQFPTFSESLINKQLEERMFIAQTITSLVLAL
                     RKKVNLKVRQPLSSIMIPVLDNNMKEDIEAVKELILNEVNVKSIQFVDNSAGILVKRI
                     KADFKKLGPKCGKHMKQVSTLLQEMSQPQIAEFEKIGNYTLSVENNVNITISTQDVEI
                     ISEDIPGWLVANEGKITIALDITLTDELKEEGIARELVNRIQNIRKANGFDITDRISI
                     VIEKNPLFNQAIENYKQYIASQTLAKSIEISDKINNGIELEFENDKLYLLIQKIA"
     gene            complement(10082..10543)
                     /locus_tag="PLD76_00340"
     CDS             complement(10082..10543)
                     /locus_tag="PLD76_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOU26487.1"
                     /translation="MIQRIQSVYLLIISILGVCLWFFPIASITHDIHVYNITTWGIQL
                     TESNQTIYQFIPYLFLVLLLPIISFSSIFIYKKRILQMRLNVIAMILMIFLYGVIYFY
                     IDFATTIISKNSIIDYKLPIIIPVINLILCYLAIRAIGKDEALIRSLSRIR"
BASE COUNT         3452 a         1745 c         1746 g         3788 t
ORIGIN      
        1 ggcaaagatt ttgacaaaat agcaactggt cattatgccc aaactttaat taaagatgat
       61 acatattatt tgagtacagc tgttgataaa cataaagatc aaacgtattt tttatctcgt
      121 atttcatacg agcaactttc taaatctctt tttcctattg gtgatttgcc aaaatctcgt
      181 gttagagaga tagcttctct tcaacaatta cctagtgcta cccgaaagga tagccaaggg
      241 atttgttttt tagggaaaat caattattca gattttataa aacgatatgt aggagttaaa
      301 aaagggcata ttattgaata cgaaacggga aaagtagttg gtcagcataa tggcgtttgg
      361 tttcatacta ttggacaacg aaaaggattg ggattaagtg gaggtccatg gtatgtggtt
      421 gcaaaacagg tggatgaaaa tattgtgtat gtttctaatg gatatgatgt tgttacacag
      481 tatggtatga tgattccact tcaagattta catattatat caggtaatcc aactatatac
      541 gacaatccca ctcgcgtagc ctttaaaatt cgtcatactc ccgagtttac ttttggtaca
      601 atacatagaa agggaaatac ttattgtatt gaatctgaag aaaaaattca gggtattgct
      661 ccaggtcagt ttggtgcatt atacgatgag aaatgtgaaa tatgcgttgg aagtggtatc
      721 attgcagagt ataaactctg attgtgatta atatataggt atatatacca aaaaaacgag
      781 gtaaagcagt tagtttcttt atctcgtttt tttgtgattc cgaagcgatt cgaacgcttg
      841 acccacgcct tagaagggcg ttgctctatc cagctgagct acggaacctc gttttgtggg
      901 cacaaaggta gaaaaaattt ttttctttgc caatttttta tagttttaat tttattagaa
      961 aggatagcct acagcaaaat ggagtgctgt atctttcata atgttccaat tactcgaaaa
     1021 ccgccaagca tcagaaccta tttttgatgg gtcatatgct ttaatccccc agtctaaacg
     1081 taataaaaaa taagagaaat cgaatcgaat tcctattcca taacctagtg caatttctct
     1141 atatatatta ttgatatcaa aattgccacc cacttgttct ccatatggta aaagagtcca
     1201 cacattacct gcatcaataa atgctcctaa ctctaacaac catactagtt ttgttctata
     1261 ttcaatatta aaatcagcac gcatatcgcc agattgactc atataatctg taattccatt
     1321 attgttataa gaaccaggtc ctaatgtacg tacttgccat cctcttacgc tattagcacc
     1381 cccgccatag tatctttttt caaaaggtaa aatggaacta tttccataag gaattccaat
     1441 acccattgca atatgatata ctattcgatt gctaggatca agaaactgag tataactata
     1501 ttctccatct agttttatgt attgcgaaaa tttaatgttc cctattacat aactattttc
     1561 ttgttttggt gttttgagta aagatgctat tccataaaaa ctatttccgg cagtttcaat
     1621 acctgctttt gcagaaaaac cgtttcgtct ttgttgtaat aatttacttg tatatgaaaa
     1681 agaataccct gtacgtaaga ttaagtggtc ttcataacta tatttcaata aagtagagct
     1741 atttagatat ttatttctaa attcatcaga aatagaatag ggtggtaaat atacatagct
     1801 taaatcaaga aagtctagtg tatgaaacat atttgattgg cggtagctat cccatctgta
     1861 ttttaaccca attccagcaa tggttcgagc gaagtcaggt cgggtttgat agctataatt
     1921 gagattaaat tcagtattag cgttactatt acgctgaaaa tctctatcta caaaaggaaa
     1981 cataagtcga ggaaattgca aggtagtttc accaccaatt tcccaagcac tatatgataa
     2041 tatattacga atgctaccaa tagcttcgtt tgatgctctt aattgtaaac caaacttttc
     2101 tccccctgta aatgcattac gatgtgtata accaaacttt ccagcaatac caaaatcacc
     2161 agcagaattt gtcccttctg cttcaatagc aaatgattga ttgttactgg gtgttaataa
     2221 aattgtacaa tctaacttgc ggatactatc agagagtaaa ggggtttcgc taaaatcaat
     2281 gcgtaagaat tgagacacat gtagattatt gattcttcga taggtttttt cggtatcata
     2341 ttctgaataa ttatccccaa ttgtaataaa cgtattatca tctaatattg atgggttgat
     2401 aaataaattg ttgtaaatta ttcgtttatc tttataaaaa agggtgtctt tttttgtttt
     2461 ttgattggta tatccgttaa tgtccaatac atcatttact aaaatatatg tgatgctatt
     2521 aatggaaaaa acctcatgat tgctacgcaa tacagtgttt ctttctcctg tacgttccat
     2581 ttttcgtata cgtataataa catctactga attagtatta agcgaactat ccgctaaaaa
     2641 tcgcacatat tctttattaa aatagtagta cccattattg cgtaataatc tagtaattct
     2701 aacacgttcg ttatctaaca catccacatc aaatagattt cctttgcgta taagagaaga
     2761 actagaatct ttttctatca agtcttttat aataggatct tgtatacgat attctatggt
     2821 atttattaca tatggattat tactaataat gttgtaagtt acttttgctt tacgtttatt
     2881 aaaaacgacg gaagattcaa cttgtgcatt ttcaaagcct ttgtttttaa aaaatcgttg
     2941 taattcaact tctgaacgtt ctgttaacgt tgaatcaaaa ataatggggg gatttccaat
     3001 tcgctttaaa aaacgattaa tccatacatc agtattgttg cctgataaat tataaatacc
     3061 taaattaaaa ctaattaaat caaacatttt tgaattaggt gtctgacgta agaatgtttt
     3121 cagttccgtt gatttaaaat tttttatatc agatttaata cgtacattgt caagtaaata
     3181 cttgttctct ggtacgtact tggtagcact acatgcactt agtaatacga taattagcag
     3241 gcttatatac cgaatgacac gactcataac ttaattttgc acaaatttac cacaaaatta
     3301 aaataataac aattactttt gcgtaataga cgataaaaat aataattatt ctcaatattt
     3361 atgttaagta aaagtaaaat aaagtttata cgttctttac gttctaaaaa gcaacgaaat
     3421 gacctacgat tatttattgc tgaagggcaa aaaacagtag atgatttatt gttatacttt
     3481 ccatgttcta tgttgtttca tacgagcgag tattgcacta gtcatagcgt ttcttatcta
     3541 caagaagtaa cacctgaaga attaaaacaa ataagttttt tagaggaacc tcaacaggtg
     3601 ttaggggttt ttgtaatgcc tgaaccaata tggtctaatg attatttaga aggagaattg
     3661 ataatagcgt tagatatgat acaagatcct ggtaatttag gtactatact tcgtgtagca
     3721 gattggtttg gtattaagca tattgtgtgt gctcgtggta cagtggatgt ctttaatcct
     3781 aaaacgatac aagcaactat gggtgcgatt ggtagggtac atgtatatat tgtagatatg
     3841 ggtgagtttt tgcaacaatt acctcacaaa attcctgttt atggaacttt tcttgaagga
     3901 gaaaatattt actctacaaa tgtgcagaac gaagctgtta taattatggg aaatgaaggg
     3961 aatggtatat cacaagcaat agaaaaacat gttactcata gaatcactat tccatctttt
     4021 cctcccaatc aaagtacttc tgaatcgctt aatgtggcta tggctactgc aatagtttgt
     4081 gcagaattta ggagacgact tttctaatag aagagtgtgc cgtaacaata gatgaaacaa
     4141 aatctatatc aagaagaaca tataattcag ataggtgagt gccaatttta ataggttgag
     4201 cccccatgtt acttaaataa tcgtatattg tttttccact attgtttgtt tgccattgta
     4261 ttatatcctc tattgaatcc ttcggtgtgt tctgtaagga aaaaataatt ccattataaa
     4321 attgagtaga aggagtttcg tgatttaatg gaagaatgtt ttctacacat aaggaaacgt
     4381 ttaaaacaat catgtgtttt tttaatattc ttttattaga aacaactata taatgggctg
     4441 ctattcgttt catgcacaca ttgctatttc gtaatttgct ccatttctac attaaaaatg
     4501 cgaatctcta ttggaattgg attatctatt ttatctatca aataacataa atctccactg
     4561 atcattgcta tttgtttttt tgagtcgggt tgaaatctta aaatttgctc aaagtcgggg
     4621 tttgtagtta caattttttt ttcaaaaatc gattctgtag agtcggtaaa gtgaatttta
     4681 acttgcatta ctagttgtaa agaagatggt acgtttttac acgaaaattt tgtttttaaa
     4741 acaaattcat ctccaagtga aaaacgaata gtagagtttt cttcaattcg aaaaggaaca
     4801 ttgctatttt gtagggatgt aagttgatat tcattttttt tctcccagat atctaaggtt
     4861 cttaaagctt gtccacccgt atttgcattt gttctaacca tttccaacga gtgttcgagt
     4921 cttggaatga tttgatcata tactctaata taggtagtgg gatgtttggc ataccaagaa
     4981 atagagctat caagctgatt aagtgtaata ttgtgttttt caagaatata attgtagtac
     5041 atatttgcac gttgattgtt gtcttttaat tggttatgac gagccatcgc ttctaccata
     5101 tgtacatcaa ccaatatatc aaccattttt tcttcagaaa taacagaaga aggaactctt
     5161 tcttcttttg tacaagaaag aaatgtacta gttacaaaaa atagagaaag aacgaaaata
     5221 agtgatttca tactattatg tttttattga atctttttgg atgatgggct aaaaaagatt
     5281 tcataaacaa gaattaatat gacgccaata gtaattgagg catctgcaat attaaatact
     5341 ggtgcaaaga aagtaaacga tgttcctcca actataggta tccaagacca gtaagtacca
     5401 ctaattaggg gaaaatatag catatctaca acttttccat aaaaaatagg ggcatatcct
     5461 tgtccccaag gtgtccaagt ggcattagaa aataacgtac tttctgtaaa aatttgtcca
     5521 taaaataaag aatcaataat attcccaata gcacctgcga atattagaga tagtgatatt
     5581 ataaatagtt gttcgctatt gttttttatc gatttgtata tgtaatatgc gatcccaatt
     5641 gatgctacta ttctaaatag ggttaaatat tttttatcaa aaaaagacat tccaaatgcc
     5701 atgccttcgt tttcaataaa atgcaaataa aagaaattag taatttttat ttcatcgcca
     5761 atagcaaaat gtgtttttat ccaaattttt actatctgat ctaaaagcag tattgtcaaa
     5821 atgattgtaa tgcttatgat ggatatagta agcttatttt ttttcatcgt ttattcgtct
     5881 taattataga caatatcgtt gtttatattt cgtttattcc acattagttt gcaaaggtaa
     5941 tcattattca gtttaaatta cagaacttag gaataacttt aactctaaaa aagatatcga
     6001 caagaccata aagtacagcc ctgtcgataa tttttgtaat ataactaatt tgtttacttt
     6061 ttccctgatt gttttgcttc aatactaagt gttgcatgag gaactgcacg taatctttcc
     6121 tttggaatta acgctccagt ttctctacaa ataccatatg ttttgttttc aattcgcact
     6181 aatgctgctt gtaaatgctg tataaatttc atttggcgtt gtgctaatct gccagcttct
     6241 tctttagaca tagtagtagc accttcttct aatactttaa aagtaggaga ggtgtcagtg
     6301 gtgtcattac catccaaatt gtttaaagca gctttcaatt gttcgtaatc aacaaatgct
     6361 ttttctaatt tatcttttat aatagctcgg aattcttcca attcgctatc tgagtatcta
     6421 gtcttttcgc ccatagttgt atgtattagt atttaatata aagtttactg cgaatataac
     6481 tccttgtttt cacatttcaa aatatattcc tcaaaaaaat atcaatatca agcaattttt
     6541 tgaattaata ggtatagttt gtcattttca aactccaact caattccatt gtttatttta
     6601 tccgaaattt ctatgctttt agctaatgtt tgagaagcta tatattgttt ataattctca
     6661 attgcttggt taaagagagg atttttttcg attacaatcg aaattctatc tgtaatgtca
     6721 aacccatttg ctttacgaat attttgtata cggtttacca attctcttgc aattccttct
     6781 tcttttaatt cgtcagtcaa agttatatct aatgcaatgg ttattttacc ttcgtttgca
     6841 accaaccagc ctggtatgtc ttctgatata atttcgacat cctgtgtgga tatagttata
     6901 tttacgttgt tttcaacaga caaagtataa tttcctatct tttcgaattc agcaatttgt
     6961 ggttgagaca tttcttgtag tagggtggat acttgtttca tgtgtttacc acattttgga
     7021 cctaattttt tgaaatcggc ttttatccgt ttcaccaata ttcccgctga attgtctacg
     7081 aattgaattg attttacatt tacttcgttt aaaatcaatt ccttaactgc ttctatgtct
     7141 tctttcatgt tattgtccaa tactggaatc ataatagagg aaagaggttg acgtactttt
     7201 aaatttactt tttttcttag agctaatact aaagacgtga tagtttgggc aataaacatg
     7261 cgttcttcaa gctgtttatt tatcaacgat tctgaaaaag taggaaattg ggctaagtgt
     7321 attgattctg aaatatcttt ttgtgttaca ctatttaaat caagaaataa gcgttctgca
     7381 aaaaatggag ctataggagc cataagtttt gccactgttt ccaaacaggt atacaatgtt
     7441 tggtaagcag ctagtttatc tagcgtcatt tctcctgccc agtagcgtct gcggtttaat
     7501 cgcacatacc aattgcttag attatctgta acaaatgtag taatggctct tccagcacgt
     7561 gttggttcgt aatcagcata attttcatct acttctttaa ctaatgaatg aagcaaagag
     7621 attatccatc tgtcaatttc ttgacgttgt tccattggaa cctctggttc tttgtatgca
     7681 aaaccatcta cattagcgta taaaacaaaa aaagaatatg tattgtataa ggtgccaaat
     7741 agtttgcgtt ttacctcttc aatgccttca gtatcaaatt ttaagttatc ccaaggagat
     7801 gcattggtca tcatatacca tcgtagggca tctgaaccat aagtttcaat agtgctaaat
     7861 gggtctactg cattacccaa acgcttagac attttattgc catttttatc taatactaag
     7921 ccgtttgaaa ctacattttt aaacgccacc gagcctttaa tcatcgtaga aatagcatgt
     7981 agagtaaaga accatccacg tgtttgatct acaccttctg aaataaaatc agcaggaaaa
     8041 ttagtctgtt tatcaattaa ttctttattt tcgaaaggat agtgtagttg agcataaggc
     8101 attgcaccag aatcaaacca tacatcaatt aggtcggttt ctcttttcat tggttttcct
     8161 gatggagata ctaatatgat gttatctaca taaggtctat gtaggtcaat acgagatatt
     8221 ccataattat cttcagaaaa atcattgaca acaaaatctt taaaaggatt tttattcatc
     8281 atccccgctt gaatggcttt ttcaatttca gccattaatt cggcaattga accaatacaa
     8341 atttcttctt gtttatcctc tgtccgccat attggtaggg gtgttcccca atacctacta
     8401 cgagataaat tccaatcctg aagattttcg agccattttc caaaacgtcc tgtaccagta
     8461 gcttgtggtt tccaatttat tgtgttattt aattcaacca ttgtttcgcg acaagctgta
     8521 gagcgtataa accagctatc tagtggataa tacaatacag gtttgtctgt acgccaacaa
     8581 tgtggatagt tatgtatatg tttttcaatt ttaaaagcta gtccttgttg tttgagcatt
     8641 acgcacaaat cgatatccaa cgttgcatca ttctcattta gcgaggtgtc gtaggcattt
     8701 tttacatatc ttccagcaaa agggctatat agggctatat ttgttctttg agctacaaaa
     8761 tcattatcta tatcgttaat agtataaaac ttcccctgaa ggtcaactaa ggggcgttca
     8821 ttaccatttt tatctatcaa taacatagca ggaatgttgt tcatttttgc tacacgggca
     8881 tcatcagcac caaaagtggg tgcaatatgt actatacccg taccatcttc ggttgttaca
     8941 aaatctccac taataacgct aaatgctcct tcatcaggct taaagaaagg tatgagctgt
     9001 ttgtaagtca taccaactag ttcacttcct ttgtattcag ctatgatgga atatggaatt
     9061 aatttatctc ctgctgtgta ttcggataat gataggcttg aggcttttcc attaaaatgg
     9121 gcagatacca aatctttagc tagaataaca gtttgaggtt caaaggtata agggttatag
     9181 gtttgcacag ccacatatgt tattgttgct ccaacacaaa gggctgtatt tgatggcaaa
     9241 gtccatggtg tagtagtcca tgctaagaaa tatgcttttc caaattgggt taattctgca
     9301 atagggttat ctatttcaaa ttgagcaact acggtagtat cttttacatc tttgtaacat
     9361 cccggttgat ttagctcgtg cgaactaagc ccactaccag cagcaggcga gtagggttga
     9421 atggtatatc ctttgtataa taaattttta ttgtacaatt cttttaataa ccaccaaata
     9481 gattcaataa atttattatc ataagtgata tatggatggt tcatatccac ccaataaccc
     9541 attttgtggg ttaaactttc ccattctttg gtatatttca tcacatcctt acgacatgcg
     9601 gcattgtact cttctactga tatagattta ccaatatctt ctttggtaat acctagagat
     9661 ttttccactc ctaattctac tggtaagccg tgagtatccc atcctgcttt gcgttgtact
     9721 tgataccctt tcattgtttt gtaacgcaaa aataaatctt taatggtacg tgccattacg
     9781 tggtgaatgc caggcatgcc gttggcagaa gggggtcctt cgtaaaaaac aaaagtagga
     9841 tttcctgttc ttgtatcaat acttttttta aaaatgtcat gtttatccca gtaggcaaga
     9901 atatctttat ttattgccga gaggtcaaga tgtgtatatt ctgaaaattt tgtattcata
     9961 tcattatttt ttcaatttgc aaagataaaa aaacaactta gaacaaaaaa agatattttt
    10021 acaaaaaagc ttcttctttt atagataaga agcctttttg tagttaacga tatgtcattt
    10081 tttagcgtat cctagaaagg gatcgtatca aagcttcatc tttaccgatg gctctaatag
    10141 ccaaatagca gagaataaga ttgataacag gtatgataat tggcaatttg taatcaataa
    10201 tgctgttctt gctaataata gtagtggcaa aatcaatata aaaatagatt actccgtaga
    10261 gaaaaatcat caatatcatt gctataacat ttaatcgcat ctgaagtatt ctttttttgt
    10321 atataaaaat acttgaaaaa gaaattatag gaagtaataa tactaaaaat aaatagggaa
    10381 taaattgata tatggtttga tttgattcag tgagttgaat accccacgtg gttatattat
    10441 acacatgtat gtcatgtgta atagatgcaa ttggaaaaaa ccaaaggcat actcctaata
    10501 tgctaataat taataaatat acggattgaa tgcgttgtat catagtagaa ttagaataga
    10561 aattagtatg ttcataaaaa aagtccgtag ctttagcagc acacggactt tagttttgtt
    10621 ggaagtgtaa attattcagc aactactgca gctgaatcaa caactacaac agctgaatca
    10681 acaacaactg cagctgaatc aacaacaact tcttctgcaa cttcttctac t
//
LOCUS       DAOSMY010000006        10479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_6348_length_10479_cov_3.716136, whole genome shotgun sequence.
ACCESSION   DAOSMY010000006 DAOSMY010000000
VERSION     DAOSMY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6348_length_10479_cov_3.716136"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLD76_00345"
     CDS             <1..394
                     /locus_tag="PLD76_00345"
                     /inference="COORDINATES: protein motif:HMM:NF039827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HOU26488.1"
                     /translation="LLIASLLGCVLPGISFWLIVSMLLYVIGPMYFCILQNAVYCKKR
                     VDILAKPHRFQGFEGFSVYAFLNVIAYIILIVTVGIISKIISEKAAIIYMVVTGITFT
                     ATSPIWIRNIYNRFLKRKYQNMEAFRND"
     gene            413..1111
                     /locus_tag="PLD76_00350"
     CDS             413..1111
                     /locus_tag="PLD76_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU26489.1"
                     /translation="MINVNNVTKSFNKKVAVNIPYLEIKKGEIIGLVGNNGAGKTTFF
                     RLLLDLIKADSGYINSNGIDVAKTEDWKVYTGSFIDNSFLIEFLTPEEYFTFIGKLFQ
                     LKGSELNERLSLFEKFMNGEILDQKKYIRNFSAGNKQKIGIIGAMMINPKILILDEPF
                     NFLDPSSQAEIKKLMQTMNSNYGTTTLISSHNLNHITDLSTRILLMEKGAIIKDIPNK
                     NGEAFTELESYFEV"
     gene            1153..2175
                     /locus_tag="PLD76_00355"
     CDS             1153..2175
                     /locus_tag="PLD76_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26490.1"
                     /translation="MQYKKQNTPLLLFILLITILSSCDKKVSYEGRIIKDYKWKAIKH
                     FSIEENCVTTAKANDSCIVLTVCNKIKKFDYSGHLLETIVPDSTNYICSYDKEGSNYW
                     VNDDNSREVKYYNKNNKLLKRQLIDTQSRIQYFGRNRFIVPDLDEKKNIYTMTLYDAI
                     KDTVLKEINICKLTGVLDTIPVRGGSITFTNSFCKNEKGQVLAYYRYNSSFIFIDTSF
                     VATAYQDFRKLPVGIPSLKGHNVLLNPINCGIDCAAMDDKFIYLVTPTYQCKQWKKSE
                     NNRMLDVYDICTKKYIGSMKLPISKGKSVFSIAKTAKGFVVFYVGNYSGIEIVIFKND
                     FTNLFN"
     gene            2202..2651
                     /locus_tag="PLD76_00360"
     CDS             2202..2651
                     /locus_tag="PLD76_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26491.1"
                     /translation="MISKIIGYFFKITVLFYNVLFLICAVSCISISLLQDGVDYMGTF
                     QVYLGDFAPKISFYIEMIVLLAATICIIAEKEFWVGWLIEILAFLPLLYAFYMDAYVY
                     LLPENQLDVFIIPSSLMIWITTVTLVFAIGGLILSLKTNNKNLSTIH"
     gene            2665..5469
                     /locus_tag="PLD76_00365"
     CDS             2665..5469
                     /locus_tag="PLD76_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU26492.1"
                     /translation="MKKLFVFILAFICLSTSYAQQQQLPIIPTDPQVLIGQLPNGMTY
                     YIRKNSLPQERADFYIAQKVGSVIEEPNERGLAHFLEHMAFNGTKHFPNKDLLNYLQR
                     IGVKFGENVNAYTSVEQTVYMVTNVPVVRETVIDSCLLVLRDWANGITLSDTEIEKER
                     GVIEEEWRSRNSAWLRMAETILPTIYGNDKYADCLPIGHIDVIRNFKPDELRAFYKKW
                     YRPDLQAIIIVGDFDPQQVEAKLKATLGTIAMPSNPIPRPVYAVTDNQEPIIAFARDK
                     EATAISVEVSIKQDVFPTEMKGTIAYIMQEYVNSAVSGMMGSRFNEMIQKPNPPFTQA
                     YAYTDNFGVSNTKDARTLYAIASEQGATTAIKSLITENERMRQFGFTIAEYEREKANI
                     LKNIENQYNEREKQQNYEYVPKYINHFLENTPIPSIEYTFETYKQLAEMIPLELINNI
                     VKQASTTGNMVIVVQGPEDEKISFPTAEEILSGIEEVKRATIEPYTENVSNEPLISKL
                     PKPGKIKKEITHSNGTTDFILSNKVTVRLVPTNHKDDEILFQAYSHGGYSLVDKQYAT
                     TLKVLPELSTLSGLGNFSAIDLQKRLSGKNLRLSKQLNTYSESLNGYSGKKDVEMLFQ
                     LAYLHFTGIRSDQDAFQAYINRQSAVLKNRENSPYTTFIDTINHVLYDSHPLMTRLKS
                     TDLSTIDYQKGMKILSERYANAADFSFVIVGNIDSETLRPLLTKYIATLPAKGKKETW
                     KDAGVTIKEGNNTLHYNKIMEVPKSSTIQYTTGKIPYTLENRIKLTILSQILDIVYTE
                     KVREDEGGTYGVSVDGNLDAIPYNRFTLETYFDTDESKRSKLVEIINQEFLAIASQGP
                     RTQDLQKVKEFLTKQYQENLIENWYWQNVEIEKIITNLDIHTDYLTILEKIDEHAIQE
                     FANFIVQTSNKKEIVQIGTK"
     gene            5631..6659
                     /gene="rlmN"
                     /locus_tag="PLD76_00370"
     CDS             5631..6659
                     /gene="rlmN"
                     /locus_tag="PLD76_00370"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU26493.1"
                     /translation="MIKKALFGKNLSEITEIVSANGLPAFAAKQITDWLYKKEISDIE
                     EMSNISAANRKKLADNYCVGIEKPVQVSVSSDGTKKYVFQVGNKHFIESVFIPDKERN
                     TLCISSQIGCKMNCLFCATGKQGFSGNLTANQILNQMRSIEEFNSLTNIVFMGMGEPL
                     DNITEVLKAIDILTSSYGYGWSPKRITVSTVGVLAPLKQFLEKSQCNLAISVHAGNSF
                     VRKELVPLEKTQPMQEIMALVKNYDFTHQRRLSIEYILFDQLNDTEEQALLLASMLKQ
                     IPCRINLIPYHRIPQVGLMPSSREKIENFQKILMKKGITTTIRRSRGQDIEAACGMLS
                     TKFVNTSN"
     gene            6669..7649
                     /locus_tag="PLD76_00375"
     CDS             6669..7649
                     /locus_tag="PLD76_00375"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOU26494.1"
                     /translation="MKNVVLLILTSLLLLTGCDEKSLKPTITGSAFEILLVVDEPIAA
                     SEAGKTLFNILNQDMYGLPQSEPLFKVSRTPHSGFTAILRPARNIIQLEINDTYSRTR
                     IVTQNDVWAAPQAVIKITSNSVEAAQQAIEKYQQDILDFFVTAERDRTIAYQKRYAEK
                     DAMKKVQDKFGISIVLPKGMNRFKEEENFLWISNASDDVRQNIVIYTYPYTSKETFTR
                     ENLLHKRDSVMLLWVPGPNPDSYMATEYRVDPPILNEIAINGTYAAELRGMWRVQGDL
                     MGGPFVNITRLDEKNQRVVTVEAFIYAPTKNKRNAIRQVEALLHTVEFTN"
     gene            7675..8679
                     /gene="pdxA"
                     /locus_tag="PLD76_00380"
     CDS             7675..8679
                     /gene="pdxA"
                     /locus_tag="PLD76_00380"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOU26495.1"
                     /translation="METKKLKIGITQGDINGIGYEIILKTLDDARILEFCTPILYGSP
                     KVAAYHRKNLEITLNLNLITNAAEANEKRVNIINCNDDEIKVELARSTDEAGKAAYLA
                     LEKATQDLKSGAIDALVTAPINKKNIQSDEFTFPGHTEYLEEKFGNKGEALMLMVNDF
                     MRIAVVTGHIPITKVAQAITTEKIVEKIHQLNDALKQDFMLTKPRIAVLGLNPHAGDD
                     GVIGDEEKTIILPAIEKALQEGIICVGPLPADGFFGASNFHHYDGILAMYHDQGLIPF
                     KALSMDSGVNYTAGLSVVRTSPAHGTAYDKAGQNLANESSFREALYLACDIVNNRQRS
                     "
     gene            8716..9936
                     /locus_tag="PLD76_00385"
     CDS             8716..9936
                     /locus_tag="PLD76_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU26496.1"
                     /translation="MDEKELQAIKQRFQIIGNTPALNRAIEIATQVAGTDLSVLITGE
                     SGVGKESFPKIIHQFSPRKHEKYIAVNCGAIPEGTIDSELFGHEKGSFTGALNDRKGY
                     FEVADGGTIFLDEVGELPLPTQARLLRVLEVGEFIKVGSSNVLKTNVRVVAATNLNIP
                     KAIQEGKFREDLYYRLNAVQIAVPPLRERKNDIPLLFKKFASDFAEKYRMPTIYLTSE
                     AQEVLVDYYWQGNIRQLKNIVEQLSIIEEHREITSDCLQNYLPKPTKEQLPLLIANTH
                     NNEKSFSNEREILYQVLFDMKKDIDELKKMVHQYKQPPVTPTKNTDNEPSFLPSIVDK
                     NTLPKTEKHIKEVEIEDAQEYEEHNFSVNEMEKNLIKKALEKHNGKRKLAALDLKISE
                     RTLYRKIKEYDLDA"
     gene            9960..>10479
                     /locus_tag="PLD76_00390"
     CDS             9960..>10479
                     /locus_tag="PLD76_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOU26497.1"
                     /translation="MKKTRFTSIGYLIIPLLIAVMGVSCSVSYKFSGTSIDYSRTKTI
                     YVRDFTNQAQMVYAPLTDVLNETVKDIYARQTRLSLVNRNGDLEIEGEITNYDISSMS
                     IQADAIAAETRLTIRVKVRFTNNKNHDEDFERTYTSFRNFSSNLMLNDVQDELITEMT
                     KEILAREVQDTVH"
BASE COUNT         3756 a         1920 c         1860 g         2943 t
ORIGIN      
        1 cttactgatt gcaagtttac ttggatgcgt tctacctgga atttcttttt ggcttatcgt
       61 aagtatgctt ttgtatgtga ttggccccat gtatttttgt attctccaaa atgccgtgta
      121 ttgcaaaaaa agagtcgata tactcgcaaa accacataga tttcagggtt ttgaaggatt
      181 ttcagtttat gcctttctta atgtaattgc ttatataatc ttaattgtta ctgtaggaat
      241 tatctcaaaa ataatttccg aaaaagctgc cataatatac atggttgtaa cgggaattac
      301 ctttacagca accagtccaa tttggatacg aaacatttac aatcgctttt taaaacgcaa
      361 ataccaaaac atggaagctt tccgaaacga ctaaaactct agcatataaa atatgataaa
      421 tgtcaataat gtaacaaaat cgttcaataa gaaggtagct gtaaacattc cctatttgga
      481 aatcaaaaaa ggagaaatta ttggattagt aggtaacaat ggagcaggga aaacaacatt
      541 tttccgatta ttgctcgatt tgattaaggc agacagcggc tacatcaata gcaatggaat
      601 tgatgttgca aagactgaag attggaaagt ctataccggg tcatttatag ataacagttt
      661 tctaatagaa tttttgaccc ctgaagagta ttttactttt attggcaagc tattccaatt
      721 aaaaggttcc gaactcaacg aacggttatc tctctttgaa aaatttatga atggagaaat
      781 tttggatcaa aaaaaataca ttagaaattt ttcggcaggt aataaacaaa agataggaat
      841 tataggggca atgatgataa atcctaagat actgatattg gatgaaccat ttaattttct
      901 tgatccgtcg tcacaggcgg aaattaaaaa attaatgcaa acgatgaact ccaattatgg
      961 taccaccaca ttaatttcga gtcataactt aaaccatatt acggatttat ccactcgtat
     1021 attactcatg gaaaaaggag caattataaa agatataccc aacaaaaatg gtgaagcgtt
     1081 tactgaattg gaatcttatt tcgaagtata aaattattct ctattgtaaa acaaattcaa
     1141 cgacctattc tcatgcaata taagaaacaa aataccccat tacttctatt tattttactg
     1201 ataaccatat tatcatcttg tgataaaaaa gtttcttacg aaggaagaat cataaaagat
     1261 tacaaatgga aagctataaa acatttctca attgaagaga actgtgtaac aacagcaaag
     1321 gcaaacgaca gttgtatcgt gctaaccgtt tgcaataaaa taaagaaatt tgattattcg
     1381 gggcatttgt tggaaaccat tgttcccgac agcaccaatt acatatgttc gtatgataaa
     1441 gaaggttcca attattgggt aaacgatgat aattctcgtg aggtaaaata ctacaacaaa
     1501 aacaataaac tactaaaacg tcaattaatt gacacacaaa gtagaatcca atatttcggg
     1561 agaaaccgat ttatagttcc ggatttagac gaaaaaaaga atatttacac aatgacgttg
     1621 tatgatgcca taaaggatac tgttttgaag gaaattaata tttgtaaact caccggagta
     1681 cttgatacga ttccggtaag aggtgggtct ataactttta caaacagttt ttgcaaaaat
     1741 gaaaaaggac aagtattggc ttattaccga tacaacagtt ctttcatatt catagacacc
     1801 tcatttgtag ctaccgctta ccaagatttc cgaaaattgc ctgtcggtat tccttcatta
     1861 aaaggtcaca atgtgctgct aaacccaatt aattgcggaa tagattgtgc tgccatggat
     1921 gacaaattca tctatttggt aacccctact tatcaatgta aacaatggaa aaaatcagaa
     1981 aataatcgta tgttagatgt atatgacatt tgcacaaaaa aatatatagg aagtatgaaa
     2041 cttcccattt caaaaggaaa atccgtcttt tcaattgcaa aaacggcaaa aggttttgta
     2101 gtattctatg ttggcaatta cagtggcatc gaaattgtaa ttttcaaaaa tgactttacc
     2161 aatttattta attaatctat ttacctctaa ccaaaaccat tatgataagt aaaattatcg
     2221 ggtacttttt taagattaca gtactttttt acaatgttct gtttttaatt tgtgcagtat
     2281 cgtgtatttc gatatctctt ttgcaagacg gtgttgatta catggggact tttcaggtat
     2341 acttgggcga ctttgccccg aaaatatcct tttacatcga aatgattgta ttgcttgcgg
     2401 ccactatttg catcatagcc gaaaaagaat tctgggtcgg ctggttgata gaaatattag
     2461 catttttacc cctgctttat gccttttata tggacgctta tgtttacttg ttgccggaaa
     2521 atcaactcga tgtttttatc ataccttctt ccctcatgat ttggattaca actgttactc
     2581 tcgtttttgc cataggaggt ctcatattat cattaaaaac caacaataaa aatttatcta
     2641 ctattcatta attcttttct atctatgaaa aaactatttg tctttatctt ggcattcatt
     2701 tgcttatcaa caagttatgc ccaacagcaa caattaccca tcattcctac cgacccacaa
     2761 gtgcttattg ggcaattacc caatgggatg acgtattata ttcgtaaaaa cagtttgcct
     2821 caagaaagag ccgactttta tattgctcaa aaagttggtt ctgttataga agaacccaac
     2881 gaacgtggat tggctcattt tttggaacac atggctttta atggtacaaa acatttcccc
     2941 aacaaggatt tattaaatta tttgcaacgc attggcgtta agtttggcga aaatgtgaac
     3001 gcttatacca gcgttgagca aacggtatat atggttacca atgttcctgt ggtaagggaa
     3061 acagtaatag attcgtgcct actggtacta cgcgattggg caaatggcat tacgctgtct
     3121 gataccgaaa ttgaaaaaga aagaggtgta attgaagaag aatggcgaag tagaaatagt
     3181 gcttggctac gcatggcaga aactattttg cctaccattt atggcaacga taagtatgcc
     3241 gattgtttgc caattggaca tattgatgtt atccgcaatt ttaaacctga tgaacttaga
     3301 gccttttaca aaaaatggta tagacctgat ttacaagcta taattattgt tggagatttc
     3361 gaccctcaac aagtggaggc taaactaaaa gcaactctag gtacaatagc catgccttct
     3421 aatcctattc ctcgtcctgt atatgctgta accgacaacc aagagcctat tatcgctttt
     3481 gcacgtgata aagaagcaac tgccatatcg gtagaagtgt ccattaaaca agatgtgttt
     3541 cctaccgaaa tgaaaggaac gattgcttat ataatgcagg aatatgtaaa ttctgcggta
     3601 agcggaatga tggggtcgcg ttttaatgaa atgatacaaa aaccaaatcc gccttttaca
     3661 caagcttatg cctatacaga taactttggg gtgtcgaata ccaaagacgc aagaacattg
     3721 tatgccattg cttctgaaca aggtgctaca actgccatta aatcgttgat taccgaaaat
     3781 gaacggatga gacaatttgg atttaccatt gccgaatacg aacgtgagaa agcgaacatt
     3841 cttaaaaaca tagaaaatca atataacgaa agagagaaac aacaaaatta tgaatacgtt
     3901 cctaaatata tcaatcattt tttagaaaac acgcccatac caagcattga atataccttt
     3961 gaaacgtata aacaattagc agaaatgatt cctcttgaat tgataaataa cattgtaaag
     4021 caggcttcta ctacaggcaa tatggtaatt gtggtacaag gtccagaaga tgaaaaaatt
     4081 agtttcccta cagccgaaga aatcttatca ggaattgaag aagtaaaacg tgcaacgatt
     4141 gaaccatata ccgaaaacgt gtcgaacgag ccattaataa gcaaattacc caaaccaggc
     4201 aaaattaaaa aggagattac acacagcaac ggtacaactg actttatatt atccaacaag
     4261 gttacggtac gattagttcc taccaaccac aaagacgacg aaatattatt tcaagcttat
     4321 agccacggtg gatactcgtt ggtagataaa cagtatgcaa ccaccttaaa ggtactgccc
     4381 gaactttcta ctcttagtgg attaggcaat tttagtgcca ttgatttaca aaaaagattg
     4441 tcaggaaaaa atcttcgcct aagcaaacaa ctaaatacgt attcagaatc attaaacggt
     4501 tattcgggta aaaaagatgt agaaatgttg tttcaattag cttacttaca ttttacaggc
     4561 atacgaagcg accaagacgc atttcaggca tatatcaacc gccaatcggc tgtattaaaa
     4621 aacagagaaa atagtccgta cacgacattt atcgatacca taaatcatgt actatacgat
     4681 agccatcctt tgatgacacg attaaaatcg accgatttgt caactatcga ctatcaaaaa
     4741 ggaatgaaaa ttttatcaga acgttatgcc aatgctgccg atttttcgtt tgtaattgtt
     4801 ggaaacatag atagcgaaac cttacgtccg ttactaacaa aatacattgc taccctaccc
     4861 gcaaaaggta aaaaagaaac ctggaaagat gcaggagtaa ccataaaaga aggaaacaac
     4921 acactacatt acaacaaaat aatggaagtt ccaaagtcaa gcacgataca atacacaaca
     4981 ggaaaaatac cttatacgtt agaaaacaga ataaaactta ccattttaag tcaaatctta
     5041 gacattgtat atacagaaaa agtacgcgaa gatgaaggag gaacttatgg cgtatctgtt
     5101 gatggcaatt tagatgctat tccgtataat cgttttacac ttgaaaccta ttttgatacc
     5161 gacgaatcaa aacgatcaaa gttagttgaa attatcaacc aagaattcct tgccattgca
     5221 agtcaaggtc cacgaacaca agatttgcaa aaagtaaaag aatttttaac aaaacaatac
     5281 caagaaaatt taattgaaaa ctggtattgg caaaatgttg aaattgagaa aattattacc
     5341 aatctcgata ttcatacaga ttacctaacg attcttgaaa aaattgacga gcatgctatt
     5401 caggaatttg ccaatttcat agtgcaaaca tcgaataaaa aagaaatagt gcaaatagga
     5461 acaaaataag tccatatcag atattgctca tatcaaacag gaatgcaaat agtgttcctg
     5521 ttttttgttg taacaacacg tatatgaaag ctgaaaatga tatcctgata aatatttccc
     5581 aaattgatgt aatttttata tctttgtgtc attaaaaaaa aattaaaccc gtgattaaaa
     5641 aagcattatt cggtaagaat ctaagcgaga taacagaaat tgtatccgcc aatgggttgc
     5701 ctgcttttgc agctaaacaa ataacagatt ggctctataa aaaagaaatc tcagacattg
     5761 aagaaatgtc gaacatttcg gcagcaaacc gcaaaaagtt ggcagacaat tattgtgtgg
     5821 gtatcgaaaa acctgtacaa gtttctgttt ctagtgatgg cacaaaaaaa tatgtgtttc
     5881 aggttggtaa taagcatttt atcgaaagtg tatttatacc cgataaagaa cgtaataccc
     5941 tctgtatatc ttcgcaaatt ggatgtaaga tgaactgctt gttttgtgct actggcaaac
     6001 aaggcttttc gggcaaccta acagccaatc agattttgaa ccaaatgcgt agtatcgaag
     6061 aatttaactc gcttaccaat attgtgttta tgggcatggg cgaaccgctt gacaatatta
     6121 ccgaagtact aaaagccatc gatattctta cgtctagcta tggttacggt tggagtccaa
     6181 aacgcattac cgtatctacc gtaggggtac tagcacctct taaacaattt ttagaaaagt
     6241 cgcaatgcaa cttggctatc agcgtacatg caggcaatag ttttgttcgc aaggaattgg
     6301 tgccactcga aaaaacacaa cccatgcaag aaataatggc tttggtaaaa aattatgatt
     6361 ttacacacca aagacgtttg tcaatcgaat atattttgtt cgatcaacta aacgataccg
     6421 aagagcaagc tctgttatta gcaagtatgc ttaaacaaat tccgtgcaga atcaatctta
     6481 ttccatatca tcgtattcca caggttggat taatgccttc tagtcgcgaa aaaatcgaaa
     6541 attttcaaaa aatactgatg aaaaaaggca ttactaccac tataagacgc tcacgtggac
     6601 aagacattga ggctgcttgc ggtatgttat caacaaaatt tgtgaatact tcaaactaat
     6661 tatagcctat gaaaaacgtg gttcttctta tacttacttc cctgttatta ctgacaggat
     6721 gcgacgaaaa aagcctaaaa ccaactatta caggaagtgc ttttgaaatt ttgctggtgg
     6781 tagacgaacc gatagctgct agcgaagctg gaaaaacgct ttttaatatt ctcaatcaag
     6841 atatgtatgg cttgcctcaa tccgaacctt tatttaaggt gtctcgcacc ccgcatagtg
     6901 ggtttactgc catcttgcgt ccagctcgaa acatcattca attggaaata aacgatacgt
     6961 attcacgcac acgcattgta actcaaaacg acgtatgggc tgctccgcaa gcggtaataa
     7021 aaattacgag taactcggta gaagctgcac agcaagccat tgagaaatac caacaagaca
     7081 ttctcgattt tttcgtaaca gctgaaagag accgcactat agcataccaa aaacgctatg
     7141 ctgaaaaaga tgcgatgaaa aaagtgcaag ataaatttgg cattagcatc gtattaccca
     7201 aaggtatgaa ccgttttaaa gaagaagaaa actttttgtg gataagcaat gctagcgacg
     7261 atgttcgtca aaatattgtt atctacacct acccttatac cagcaaagaa acatttacgc
     7321 gtgaaaatct gttacacaaa cgcgattctg tcatgctctt gtgggtgcct ggaccaaatc
     7381 cagattcgta tatggctacc gaataccgag tagatcctcc catacttaac gaaatagcaa
     7441 ttaacggtac atacgcagcc gaactacgtg gtatgtggcg tgtacaggga gatttaatgg
     7501 gtggcccgtt tgtaaatatt acccgattag acgaaaaaaa tcaacgtgta gtaaccgtag
     7561 aagcatttat atacgctcca acaaaaaata aacgcaacgc cattcgacaa gtagaagcat
     7621 tgttgcacac agtcgaattt actaactaaa aaaactagct gaaaaacaat cgatatggaa
     7681 acaaaaaaac taaaaatagg tattacgcaa ggtgatatca atggaatagg atacgaaata
     7741 attctgaaaa cattagatga tgcccgcatt cttgaatttt gcacacctat actatacggt
     7801 tcgcccaaag tagcagccta tcaccgcaaa aacttagaaa taacgcttaa cctcaacctt
     7861 attaccaatg cagctgaggc aaatgagaaa cgagtaaaca ttatcaactg caacgatgat
     7921 gaaataaaag tagaactagc acgctctacg gatgaggctg gcaaagcagc ttacttagct
     7981 ctggaaaaag caactcaaga cctcaaatca ggtgcaatag atgccctagt tacagctcct
     8041 atcaataaaa agaatataca atcagatgaa tttacttttc ccggtcatac cgaatattta
     8101 gaagaaaaat ttggcaacaa aggcgaagca ctcatgttga tggtgaatga ttttatgcgt
     8161 attgctgttg ttacaggtca tattcccatt accaaagtag ctcaagccat tacaaccgaa
     8221 aaaattgtag aaaagattca ccaactcaac gatgccctga aacaagattt tatgttaacc
     8281 aaaccacgta tagctgtttt aggcttaaac ccgcatgctg gtgatgatgg cgtaatagga
     8341 gatgaagaaa aaacaatcat tttgccagct attgaaaaag cactgcaaga aggaattatt
     8401 tgcgtaggac cactacccgc tgatgggttc tttggtgcat caaattttca tcattacgat
     8461 ggcattttag cgatgtatca cgaccaaggt ttaatccctt tcaaagcctt atcaatggat
     8521 tcgggcgtaa attataccgc aggattatcc gtagtacgca cctcgcctgc tcatggcact
     8581 gcctacgaca aagcagggca aaatcttgcc aacgaatcgt cttttagaga ggcattatat
     8641 ttggcatgtg atatagttaa caacagacaa cgaagctaat ctattcaaca ctttagtgga
     8701 aaggattgta gtatgattga cgaaaaagaa ttacaagcaa taaaacaacg ttttcagatt
     8761 attggcaata cgccagcact caatagggct attgaaatag ctactcaagt agctggcaca
     8821 gacttatctg tactaataac gggcgaaagc ggtgtaggaa aagaaagttt tccgaaaatt
     8881 atacaccaat ttagtcctcg taaacacgaa aaatacattg ccgtaaactg tggtgctata
     8941 cccgaaggaa cgattgattc ggaacttttt ggacacgaaa aaggctcttt tacaggagca
     9001 ctcaacgacc gaaaaggtta ttttgaagta gccgatggtg gcacaatctt tttagacgaa
     9061 gtgggcgaac tacccttgcc tacccaagct cgtttgttgc gtgtgctcga agtgggcgaa
     9121 tttatcaagg tgggctcatc gaatgtatta aaaacaaacg tacgtgtagt agctgccact
     9181 aatttgaaca tccccaaagc catacaagaa ggtaaatttc gtgaagattt gtattacaga
     9241 ctcaatgctg tacaaatagc agtaccccca ctacgcgaaa gaaaaaatga tatcccctta
     9301 ttatttaaaa aatttgcatc cgactttgcc gaaaaatacc gtatgcctac tatttacctt
     9361 acctctgagg ctcaagaagt attggttgat tattattggc aagggaatat tcgtcaatta
     9421 aaaaacatcg ttgagcaact atctattatt gaagagcaca gagaaattac aagcgattgc
     9481 ttgcaaaact atttaccaaa acccacgaaa gaacagttac cgttgcttat agcaaacaca
     9541 cataacaacg aaaaatcgtt ttctaacgaa cgagaaatct tataccaagt gttattcgat
     9601 atgaaaaagg acattgatga actaaaaaaa atggtgcatc agtacaaaca accccctgta
     9661 actccgacaa aaaatacgga taacgaacct tcttttttac catcaattgt ggataaaaat
     9721 acgttgccaa aaacagaaaa gcacattaaa gaagtagaaa tcgaagatgc tcaagagtac
     9781 gaagaacaca attttagtgt aaacgaaatg gagaaaaact tgataaaaaa agcactcgaa
     9841 aaacacaatg gtaaacgcaa attggctgcc ttggatttaa aaatatctga acgcacctta
     9901 tatcgtaaaa ttaaagaata tgatttagac gcttagtcag attttaacgc acacaaacca
     9961 tgaaaaaaac acgttttaca tccattggct acctaattat tccattacta atagcagtaa
    10021 tgggcgtttc ttgctctgta tcatacaaat tctcaggcac ttctattgat tatagtcgta
    10081 ccaaaacaat ttacgtacgc gattttacca atcaggctca aatggtatat gctcctctta
    10141 ctgatgtatt aaacgaaaca gtaaaagata tatatgctcg ccaaacacgc ctttcgctag
    10201 tcaaccgaaa tggggactta gaaatagagg gagaaattac aaactacgac atttcgtcta
    10261 tgtctattca ggcagatgct attgctgccg aaacacgact aaccatacgc gtaaaagtac
    10321 gttttacgaa caataaaaac cacgacgaag attttgaacg cacgtacaca tctttccgta
    10381 attttagtag taacctgatg ctaaatgatg tgcaagatga attaattact gaaatgacaa
    10441 aagaaattct tgcaagagaa gtacaagata ctgtacatt
//
LOCUS       DAOSMY010000007        10173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_6597_length_10173_cov_3.681755, whole genome shotgun sequence.
ACCESSION   DAOSMY010000007 DAOSMY010000000
VERSION     DAOSMY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_10173_cov_3.681755"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1460)
                     /locus_tag="PLD76_00395"
     CDS             complement(6..1460)
                     /locus_tag="PLD76_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOU26498.1"
                     /translation="MHYTIEDLIKLPIWQHFYAISQIPRPSGRMDAITDYIEGFGKSL
                     RLETLRDKANNIVIRKPASAGYEGCKKVILQSHLDMVPQKNEGVLHDFCTDAIKWKIT
                     DNWLMATDTTLGADNGIGVAAMMAILENKELVHPPLEALFTVDEENGMNGAFGVAPDW
                     LQGDILINLDSEDEQEIIVGCAGAVRVQCNFNFEKTPVPEGDKAYKIGIKGLLGGHSG
                     IDIHLQRANANKLLFHFLKTLVVDFEARVAYIKGGTIINAIPREAEAIITIPTGGEDD
                     LMSVYHDFKAIYTQEYSHIDPDVSIEIESIILPDGLLPEPIQDDIINAVIGAPNGVYR
                     YVPLSNLVETSTNLGIIETSTNCVSTHFLVRSNVDSMKGEVRSMIESIFSLAGAKISV
                     SGNYDGWSPKSSSNLVNLVREQYEHFFDRDPEVKTIHAGLECGIIGSKYPHLDMTSIG
                     PTIKYPHSPNEKVDILSVDRFYRFLKVLLSQIGV"
     gene            complement(1479..3446)
                     /gene="dnaG"
                     /locus_tag="PLD76_00400"
     CDS             complement(1479..3446)
                     /gene="dnaG"
                     /locus_tag="PLD76_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOU26499.1"
                     /translation="MIDQVTVDRILQTAQIEDVVGEFVSLRKRGVNMIGLCPFHDEKT
                     PSFTVSPAKNICKCFGCGKGGTPVNFIMEHEHLSYYDALRYLAKKYNIEIVEKEISAE
                     ELNKRNERESLFVINTFAQTYFEDVLWKQDEGRSVGLSYFKERGFRDDVIKKFGLGYS
                     LESYDSFGRVALEKGYKRDYLLKTGLCTERQDASLVDRFRGRVIFPVHTLSGKIVAFG
                     GRILKTDEKTAKYLNSPESEIYHKSNELYGLFFAKQSIVKYDNCFLVEGYTDVLSMYQ
                     NGIENVVSSSGTALTPGQIRLIRRFTSNITVLYDGDAAGIKASIRGIDLLLEEGMNVK
                     VVLLPQGEDPDSFAKKQNASDFIQFIEKNQTDFIHFKSHLLLEEAGKDVSKKAKLIAD
                     IVQSIAIIPDAIMRSVYVKECAKQFEMNENVLFTEVNKRRILLLESQSKQTNFVVESE
                     DADNAISELPSDHSTVNAFLHYTFLQEQNLIACILRNGASKIVGQDENKAEYIISVFD
                     FILNDLAVDDLDFSYSLHQQICNELIRLRASNQEQIAFYFLNHPNKEIRDYVFELITD
                     KHTLSKVHQKYKAIESEQDRLNYQVTKYLLDFKEKIVDYYCKELRDKIKQSEGKVSDE
                     EMFSLLQELNRYNAIRNIIMKQSGEKTVIKI"
     gene            3604..4380
                     /locus_tag="PLD76_00405"
     CDS             3604..4380
                     /locus_tag="PLD76_00405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU26500.1"
                     /translation="MKTVLITGASEGIGYELSQLFANAHYQLILVARNEQKLNAMCET
                     YTSNGIKCTYFVADLSIMSHVERLADKIDQLPFKIDYLINNAGFGIYGNSTELDWEKE
                     SAMYQLNMISLAYLTKHFSIQMTKNGFGRILNVASIAAFQPLPFMAGYAASKAFVLSL
                     SQALNEELKKQNVYVSTLCPGVTESKFHNTANTEKKTLGSFLFPCCHSTRSCSVWVQT
                     TYVRQKHRHIWLAKSHSNWLVTIYPSCIYCENCWDNDEIN"
     gene            complement(4446..5201)
                     /locus_tag="PLD76_00410"
     CDS             complement(4446..5201)
                     /locus_tag="PLD76_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOU26501.1"
                     /translation="MEKTINVTLKVWRQAGPKAQGKFETYKLNNVSTDSSFLEMMDIL
                     NEQLISERKEPVAFDHDCREGICGMCSLYINGHPHGPDGEITTCQLHMRKFKDGDTIT
                     IEPWRSAAFPVIKDLMVNRGAYDKIIQAGGFVSVNTGGVPDGNAIPISKIYADEAMDA
                     ASCIGCGACAAACKNGSAMLFVAAKVSQLALLPQGKVERATRAKAMVAKMDELGFGNC
                     TNTGACEAECPKNISISHIARLNREFLSAKIKD"
     gene            complement(5238..7175)
                     /locus_tag="PLD76_00415"
     CDS             complement(5238..7175)
                     /locus_tag="PLD76_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOU26502.1"
                     /translation="MIDAKVPSGPLAEKWKNYKAHQKLVNPSNKRRLDIIVVGTGLAG
                     ASAAASLAELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRARESNVYRLAEVSGSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAKGQ
                     TGQQLLLGAYSALSRQVGKGAVKLYSRYEMLDVVIIDGRARGIIARNLVSGKIERFFA
                     HAVVVGTGGYGNAFFLSTNAMGSNGSAAIQMYKKGAYFANPCYAQIHPTCIPVHGEFQ
                     SKLTLMSESLRNDGRIWVPKKKEDAEAIRKGTLKPTDLKEEDRDYYLERRYPAFGNLV
                     PRDVASRAAKERCDAGYGVGATGLAVYLDFADSIKRLGLDVVKARYGNLFQMYEKIVD
                     DNPYETPMMIYPAIHYTMGGIWVDYELQTSVKGLFCIGEANFSDHGANRLGASALMQG
                     LADGYFVLPYTIQNYLADQIQVPRMSTDLPEFIEAEKEVNDKIQKLLSIQGQRSVDSI
                     HRELGLVMWDFVGMGRNKEGLQQAIDKIKAIKKEFWTNVYIPGKGDDLNVELEKALRV
                     ADFIEIGNLMARDALQREESCGGHFREEYQTPEGEAMRNDDDYAFAACWKYMGENADP
                     VLLKERLDYEFIQRQQRNYKA"
     gene            complement(7266..7955)
                     /locus_tag="PLD76_00420"
     CDS             complement(7266..7955)
                     /locus_tag="PLD76_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HOU26503.1"
                     /translation="MWLINSSIGRKLIMSISGIMLVLFLLFHGLMNMVVIFSADAYNA
                     ICAFLGANWYALIATKGLALGFLVHIAYALFLSYQNMKARGKERYAVSDKQDKVSWAS
                     QNMLILGLIVFGFLALHLYHFWSKMQLVEVGHMLGKHIGDIDKAVDGAYWITYWFSQP
                     VYAIIYLVWLSALWFHLTHGVWSSLHSLGLNNQTWLPRVKCISNIFATLVVLLFVSVV
                     VYTYVYQNFVL"
     gene            complement(8247..9371)
                     /locus_tag="PLD76_00425"
     CDS             complement(8247..9371)
                     /locus_tag="PLD76_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26504.1"
                     /translation="MKHLEIRTIEQVKSLFHTTDNIQYVAFQHIDFNDVSTSKFDKSY
                     SNCLFLGCILSQEFICKKLRDCTIFPSIDVPYTIFIPHLYTPHDLYAGYKIGSPDSFS
                     HTFDQQVYSHYIAKGKETSDIKETLARRIHDHSISDALHDFLVSYEPTKLVAIMGGHN
                     ITRTDIGFLEIAKMSKTLTEQSYLMLSGGGPGAMEATHFGAWMAGRTESELLHAYEML
                     SEAPHYDHLLWLEKAFMVMERYPQHTFESIGIPTWFYGHEPPTPFATRIAKYFENSIR
                     EDGLLAIAKGGIIFTPGNAGTIQEIFQDANQNHYLTFGLASPMIFYNADYWTHSRPIY
                     PLLTQLFEEGKYKNLILSVCNNQQEVLEQLAAFALSQQAE"
     gene            complement(9429..9836)
                     /locus_tag="PLD76_00430"
     CDS             complement(9429..9836)
                     /locus_tag="PLD76_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOU26505.1"
                     /translation="MYSLQYQYTFYQKNSFSSELVIQPQYNAASFFEDTQKYSFEYGV
                     NIGALLRKELSNFSVFAMLSAGPHYVSDVPSRQANGFIFSDNLMVGVSYMLTENLGLD
                     VRGGVRHISNAGFIAPNGGVNNTIFQIGFFVLP"
     gene            complement(10068..>10173)
                     /gene="rrf"
                     /locus_tag="PLD76_00435"
     rRNA            complement(10068..>10173)
                     /gene="rrf"
                     /locus_tag="PLD76_00435"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3323 a         2111 c         1455 g         3284 t
ORIGIN      
        1 cctaattata ctccgatttg agacaagaga actttcagaa aacgataaaa tctatctaca
       61 cttagtatat ctactttttc attgggagaa tgagggtatt taattgttgg acctatcgaa
      121 gtcatatcca aatgaggata tttacttcca ataatgccac actctaaacc cgcatgaatt
      181 gtctttacct caggatctct atcaaaaaaa tgttcgtatt gttctcgcac taagttgaca
      241 agattagatg atgatttagg gctccatcca tcataattcc ccgagacact aatttttgcc
      301 ccagccaaac taaagatact ttctatcatc gatctcactt ctcctttcat agaatccaca
      361 ttacttctta ctaaaaaatg ggtactcaca caatttgtag aagtttcaat aatccccaaa
      421 tttgtagaag tttccaccaa attagacaaa gggacatagc gatatacccc attaggagcc
      481 cctattacag cattgataat atcatcctga ataggttctg gcaataatcc atcaggtaat
      541 ataatggact caatttcgat tgatacatca ggatcaatat ggctatattc ttgtgtataa
      601 atagccttaa aatcatgata cacactcatc aaatcatctt ctcctcctgt aggaattgta
      661 ataatagctt ctgcctcacg tggaatagca ttaatgatag tccctccttt tatataggca
      721 actcttgctt caaaatcaac cactaaggtc tttaaaaaat gaaataataa tttattggca
      781 ttagcacgtt gcaaatgaat atcaatccct gaatgccctc caagaagccc ttttattcca
      841 attttatatg ctttatctcc ttcgggaaca ggagtttttt caaaattaaa attacattgt
      901 actcgcactg ctcctgcaca tccaactatt atttcttgtt cgtcttccga atctaaattt
      961 ataagtatat ctccttgcaa ccaatcaggg gctactccaa aagctccatt catcccattt
     1021 tcttcatcta ctgtaaacaa tgcctctagt ggaggatgca caagttcttt attctctaaa
     1081 atagccatca ttgctgcaac tcctatcccg ttatctgccc caagggttgt atctgttgcc
     1141 atcaaccaat tatcagtaat cttccactta atagcatcag tacaaaaatc atgcaatacc
     1201 ccttcatttt tttgtggcac catatccaaa tggctttgca aaatgacctt tttgcacccc
     1261 tcatatcctg cactagctgg ttttcgtatt acaatattat ttgctttgtc tcgtagcgtt
     1321 tctaaacgca atgattttcc aaacccttca atataatcag tgatggcatc catcctacca
     1381 gatggacgtg gaatttggct aattgcataa aagtgttgcc atattggcaa ttttatcaaa
     1441 tcttctattg tataatgcat gaattaaaaa agttatagtt aaatctttat gaccgttttt
     1501 tcgccagatt gtttcattat gatatttcgt attgcattgt atcgattcaa ttcttgcaat
     1561 aaagaaaaca tctcttcatc cgacactttc ccttcggatt gttttatctt atctctcaac
     1621 tctttgcaat aataatctac aattttctct ttaaaatcaa gtaaatactt ggttacctga
     1681 taatttaatc tatcttgctc cgattcaata gctttgtatt tttgatgaac tttgctcaaa
     1741 gtatgtttat ccgtaattag ttcaaagaca taatcccgaa tttctttatt tggatgattt
     1801 aaaaaataaa atgctatttg ttcttgattg cttgctctca atctaattaa ttcgttacat
     1861 atctgctgat gcaaagaata ggaaaaatct aaatcatcaa ccgctaaatc atttaaaata
     1921 aaatcaaaaa ctgaaataat atactctgcc ttgttttcat cctgacctac aatcttagat
     1981 gctccatttc gtaaaatgca ggctattaga ttttgttctt gcaaaaaagt gtaatgcaaa
     2041 aatgcattca ctgttgaatg atctgaagga agttcactaa tagcattatc tgcatcctca
     2101 gattccacca caaaatttgt ctgtttcgat tgagattcaa gcaaaagaat ccgcctttta
     2161 tttacttctg taaacaaaac attctcattc atttcaaatt gtttagcaca ctcttttaca
     2221 tatacagaac gcataatagc atctggtata atcgcaatgc tttgcacaat atctgcaatt
     2281 agtttggctt tcttactcac atctttgccg gcttcttcaa gcaacaagtg tgatttaaaa
     2341 tgaataaagt ctgtttgatt tttctctata aactgtataa aatcactggc attttgtttt
     2401 ttagcaaaag agtctgggtc ttctccctgt ggcaataaca ccacttttac attcatgcct
     2461 tcttctagca ataaatcaat acccctaata gacgctttta ttccagcagc gtctccatcg
     2521 tataacaccg taatattgga ggtaaaccta cgtatcaaac gaatctgacc aggagtaaga
     2581 gcggttcctg aagatgacac aacattctca atgccatttt gatacataga taatacatct
     2641 gtataacctt ctaccaaaaa acaattatca tactttacaa tactttgctt agcaaaaaac
     2701 aatccataca actcattact tttatgataa atttcagatt ctggtgaatt gaggtatttt
     2761 gctgtttttt catcagtctt caaaattcta cctccaaaag ccacaatctt tcccgacaat
     2821 gtgtggacag gaaaaatgac ccttcctcga aatctatcaa ccaaactcgc atcttgacgt
     2881 tcagtacaca atcctgtttt taaaagatag tcccgcttat agcctttttc taaagctaca
     2941 cgcccaaaag aatcatacga ttccaaacta taacctaagc caaatttctt tataacatca
     3001 tcacgaaaac ctctttcttt aaaataagat aaacccacgg atcttccctc gtcttgtttc
     3061 caaagaacat cctcaaaata agtttgagca aaagtgttaa ttacaaacaa actttctctt
     3121 tcgttgcgtt tatttaactc ctctgccgat atttcctttt ctactatttc aatattatat
     3181 tttttagcta agtaacgcaa cgcatcataa tatgataaat gctcatgctc catgataaaa
     3241 tttacaggcg ttcctccttt tccacaacca aaacatttac aaatattttt ggctggagaa
     3301 acagtaaacg atggcgtttt ttcgtcatga aatggacaaa gcccaatcat attcacccct
     3361 cttttacgaa gtgatacaaa ctcgccaaca acatcttcaa tctgtgctgt ttgtagaatc
     3421 ctatccactg ttacttgatc aatcatctct attttgttag gaacacaaat atacgagaat
     3481 tttactattt atctatcttg atgtaggagc ttttttctac aaaaaatctc gtatatttgt
     3541 agggcatctc aacaaaaatt tatcgcaaca agaatatcga gtaataaata aacttcaaaa
     3601 cttatgaaaa ccgtacttat aacaggggca tcagaaggta taggatacga attgtctcaa
     3661 ttatttgcca acgcacatta tcagcttatt ttggtggcaa gaaatgaaca aaaattaaac
     3721 gccatgtgtg aaacatatac atctaatggt ataaaatgca cgtattttgt ggctgattta
     3781 tccattatgt ctcacgtgga aaggcttgca gataaaatcg accaactgcc attcaaaata
     3841 gactatctaa taaacaacgc tgggtttggg atatatggaa actctactga actggattgg
     3901 gaaaaagaat ctgctatgta tcaactaaat atgattagtt tagcctattt aacaaaacac
     3961 ttttcaattc aaatgacaaa aaatggtttt ggaaggattt taaatgttgc ctctattgcc
     4021 gcctttcaac ctttgccatt tatggcaggg tatgcagcct ccaaagcttt tgtgcttagc
     4081 ttatctcaag cattgaacga agaacttaaa aagcaaaacg tatatgtgtc taccctatgt
     4141 ccaggtgtta cagaaagtaa atttcataac acagccaata cagaaaaaaa aactcttggg
     4201 agcttccttt ttccctgttg ccacagcaca agaagttgct cagtatgggt acaaactact
     4261 tatgtcaggc aaaaacatcg gcatatatgg cttgctaaat cgcattcaaa ttggcttgtt
     4321 acgatttatc cctcgtgcat ttattgtgaa aattgttggg ataatgatga aataaattga
     4381 cttcgttcaa taaaaaagct gctttgtgaa ttacaaagca gcttttttat tgacaaacct
     4441 agcggttaat cttttatctt agccgacaaa aactctctgt ttaaacgagc aatatggctt
     4501 atggaaatat ttttaggaca ctctgcttca caagctccgg tattagtaca attaccaaac
     4561 cctaattcat ccattttagc aaccatcgct ttggctcgtg ttgctctttc tacttttcct
     4621 tgaggcaata atgcaagttg acttaccttt gcagcaacaa atagcattgc tgatccattt
     4681 ttacaagccg ctgcacaagc accacaaccg atacaagagg cagcgtccat tgcttcgtct
     4741 gcataaattt ttgaaatagg aattgcattg ccgtctggca ctccacctgt gtttacagac
     4801 acaaaaccac cagcctgaat aatcttatca taagcccctc tattgaccat caaatcctta
     4861 ataacaggaa atgctgccga acgccatggc tcaatagtaa tcgtatctcc atctttaaat
     4921 ttacgcatgt gcaattgaca tgtagtgatt tcaccatctg gtccgtgtgg atgtccgtta
     4981 atatacaacg aacacatacc acaaatacct tctctacaat catgatcaaa agctactggt
     5041 tctttgcgtt cgcttattaa ctgctcattc aaaatatcca tcatttctaa gaatgaacta
     5101 tctgttgaaa cattattcaa tttataggtt tcaaatttac cttgtgcttt tggacctgcc
     5161 tgacgccaaa ctttaagcgt tacatttatt gttttttcca tcttacagaa tttctttttg
     5221 tttggatacc ttgttaatta tgctttgtaa ttgcgttgtt gacgttgaat aaactcataa
     5281 tcaagtcttt cttttaacaa cacgggatct gcattttctc ccatatactt ccaacacgcc
     5341 gcaaatgcat aatcatcatc attacgcatt gcttctcctt ctggagtttg atattcttca
     5401 cggaagtgac ctccacatga ttcctctctt tgcaatgcat cgcgagccat caaatttcct
     5461 atttcaataa aatctgctac tcgcaaagct ttttctaatt caacgttcaa atcgtctcct
     5521 ttaccaggaa tatatacgtt agtccaaaat tcctttttaa tagctttaat tttatcaata
     5581 gcctgttgca atccttcttt atttctgccc atacccacaa agtcccacat aacaagacct
     5641 agctctctat gtatactatc tacagaacgt tgaccttgta tgcttaataa tttttgaatc
     5701 ttgtcattca cctctttttc tgcttcaata aattcaggca aatcagtact cattcgtggt
     5761 acttgaattt gatctgccaa atagttttga attgtataag gtaatacaaa atacccatct
     5821 gctaaacctt gcattaaagc agatgcacct aaacggtttg ctccatggtc tgagaaattt
     5881 gcttcgccaa tacagaacaa tcctttaacc gatgtttgta gttcataatc tacccaaatt
     5941 cctcccattg tatagtgtat ggcaggataa atcatcattg gagtttcata tggattgtca
     6001 tctacaatct tttcgtacat ttgaaataag ttaccatatc tagccttaac aacatcaaga
     6061 cctaaacgtt taattgaatc agcaaaatct aaatatacag ctaaacctgt agcaccaaca
     6121 ccatatccag catcacatct ttctttggca gcacgagaag ccacatcacg aggcaccaaa
     6181 ttaccaaaag caggataacg acgctctaaa taataatctc tatcttcttc ttttaaatca
     6241 gttggtttta aagtcccttt gcgaatagct tctgcatctt ctttcttttt tggcacccat
     6301 atacgaccat cattacgcaa cgattccgac atcaatgtta atttcgattg aaactcaccg
     6361 tgcacaggaa tacatgttgg gtgaatttgg gcataacatg gattagcaaa atacgctcct
     6421 tttttataca tttgaatagc agcagaccca ttagatccca tcgcattggt cgacaaaaag
     6481 aaagcattac cataaccacc tgtccctact actaccgcat gggcaaaaaa gcgttcaatt
     6541 tttccagaaa ctaaattacg tgcaatgatt cctcgagctc ttccatcaat aattactaca
     6601 tctaacattt cgtaacgcga atatagcttc acggctcctt tgccaacttg acggcttagt
     6661 gctgaatatg cccctaataa taattgttga ccagtttgac ctttagcata aaaagtacgt
     6721 gatacctgtg ctcctccaaa cgaacgatta tctaacaaac ctccatattc gcgtgcaaaa
     6781 ggaactcctt gtgcaacaca ttggtcaata atacttcctg atacttctgc caaacgataa
     6841 acattggact ctcttgctct ataatcacca ccctttattg tgtcataaaa caaacgatac
     6901 actgaatctc catcattttg ataattttta gccgcattta tacctccttg tgcagcaata
     6961 gaatgggctc tccttggtga atcttgaata caaaagttta gtacgttaaa acctaattca
     7021 gccaacgatg cagcagctga ggctcctgct aacccagtac ctacaacaat gatatctaat
     7081 cgccttttat tcgatggatt taccaatttt tgatgtgcct tgtaattctt ccatttctca
     7141 gctaaaggac cagaaggcac tttggcatca atcataattg gtgtttctgt aggctttaca
     7201 gcctgtgttg tttctacaac agtttctttc ttagccatat tgtgtgatat atttctagtt
     7261 acaaattata aaacaaaatt ttgatatacg taagtataaa caacaacaga aacaaacagt
     7321 aatacaacta atgtcgcaaa aatgtttgaa atacacttca cacgaggaag ccatgtttga
     7381 ttgtttaatc ctaatgaatg taatgaactc catactccat gggtaagatg aaaccataaa
     7441 gctgaaagcc acactaaata gataatagca tacactggtt gagaaaacca ataggtaatc
     7501 caatatgctc catcaaccgc tttatcaata tctcctatgt gttttcctaa catgtgccca
     7561 acttcaacta attgcatttt actccaaaaa tgatacaaat gcaatgctaa aaatccaaat
     7621 acaatcaatc ctaaaatcaa catattttga gaagcccatg atactttatc ttgtttatct
     7681 gaaacagcat aacgctcttt accacgagct ttcatgtttt gataagacaa aaataaagca
     7741 taagcaatat gcactaaaaa ccctaaagct aaacctttag ttgcgattaa ggcataccaa
     7801 ttcgcaccca aaaatgcaca aattgcatta tatgcatcag cagaaaaaat cactaccatg
     7861 ttcattagac catgaaacaa caaaaaaagt accagcatga tgccagagat actcataatc
     7921 agctttcttc ctatcgaaga gttaattaac cacatacttc cagtttttta attattattg
     7981 atttaataca aaaatttaat tttcagtgta caaaagtaat aaaaagttca atgctaaagg
     8041 gtatatacta tcacattttt taacgtaatg caaacgattt ttcattcttg tagtaattct
     8101 cccaaaaata gtttaagtta ttgttattta agctaatata aaatcaattt gtagcccaaa
     8161 aaactgtatt gctttcatta agcatacaaa aataggtaaa tatatgtaaa atttgctcaa
     8221 aataacacaa caagcaggat attatgctac tcagcttgtt ggctcagagc aaaagcggct
     8281 aactgctcca acacctcttg ttgattatta catacagaaa gaataagatt tttatactta
     8341 ccttcttcaa acaattgtgt taagagcggg tatattggtc ggctatgagt ccaataatca
     8401 gcattataaa atatcattgg acttgctaat ccaaaggtaa gataatggtt ttggttggca
     8461 tcttgaaaaa tctcttgaat agttcctgca tttcctgggg taaaaattat acctcctttt
     8521 gctattgcca acaaaccatc ttctcgtata ctattttcaa aatactttgc aatacgtgtt
     8581 gcaaaaggag ttggaggttc atgtccataa aaccatgttg ggataccaat gctttcgaat
     8641 gtgtgttgtg ggtatctctc cattaccata aacgccttct ctaaccataa aagatgatcg
     8701 taatggggag cctctgaaag catttcataa gcatgtagta attcgctttc tgttctacca
     8761 gccatccaag caccaaaatg agttgcttcc attgcccctg gacctcctcc agataacata
     8821 aggtaagatt gctctgtcaa agtcttactc atttttgcta tttctaaaaa tccaatatct
     8881 gttcgtgtta tattatgacc acccattatt gcaactaatt tggtaggttc ataagaaact
     8941 aaaaagtcat gtaatgcgtc agaaattgaa tggtcgtgta tcctacgtgc taatgtttcc
     9001 ttaatatctg aagtctcctt ccctttggct atgtaatggg aatatacttg ttgatcaaac
     9061 gtatgcgaaa atgaatcagg acttccaatt ttatatcctg cataaagatc atgtggagta
     9121 tataagtgtg gaataaaaat agtataaggg acatctattg aagggaaaat agtgcaatct
     9181 cgtaatttct tacagataaa ttcttgagaa agaatacaac ctagaaacaa acaatttgaa
     9241 tacgacttat caaatttact agtacttaca tcattaaaat caatgtgttg aaaagccaca
     9301 tattgaatat tatcagtagt atgaaagaga gattttactt gttcgattgt acgaatctcc
     9361 aaatgcttca tgcccatttc ttttatttca tgtaaaaata acaaaaatat tggacttaat
     9421 cttacaaatt atggtaaaac aaagaatcct atttgaaaaa tagtattatt aacacctcca
     9481 ttaggggcga tgaaaccggc attactaata tgtcgaactc ctcctctaac atctaatcca
     9541 aggttctcag tcaacatata agaaactcca accattaaat tgtcagaaaa aataaaccca
     9601 ttggcttgtc ttgatggaac atcagaaaca taatgaggac cagcactaag cattgcaaaa
     9661 acagaaaaat ttgacaattc ttttcttagt aatgctccta tattcacacc atactcaaat
     9721 gaatattttt gtgtatcctc aaaaaaactc gcagcattat attgtggctg tattacaagt
     9781 tcacttgaaa atgaattctt ttgataaaat gtatactgat actgaagaga atacaatagt
     9841 aattaataat tataggttac atcccaaaac aattggttac cataaccaac tgcaaatcca
     9901 aatttatttt taccagttgc aatacgattc tcatcaaaag tagcagagaa tgtatagaaa
     9961 catgaaaaat aaagtaaaag caaaagtatt aaaatcttct tcatttcact attgttaccc
    10021 ctaaataaaa aaaggaggcg atgtatgaca catcacctcc ttttgaaaaa cggcggctat
    10081 ctactctccc acacaagtgc agtaccatcg acgctattgg gcttaacttc tctgttcgga
    10141 atgggaagag gtggaacccc aacgctataa cca
//
LOCUS       DAOSMY010000008         9207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_7466_length_9207_cov_3.850962, whole genome shotgun sequence.
ACCESSION   DAOSMY010000008 DAOSMY010000000
VERSION     DAOSMY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_9207_cov_3.850962"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1083)
                     /gene="pstS"
                     /locus_tag="PLD76_00440"
     CDS             complement(52..1083)
                     /gene="pstS"
                     /locus_tag="PLD76_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOU26506.1"
                     /translation="MKKLVFLLSTVTVLLSSCNTETVELAGAGATFPVPYYNVAFEAY
                     KAETGNIVSYGGIGSGGGIRSITEGIVDFAGTDAFLNDERMAEIDEVIHIPTCMGAVV
                     LSYNLEGVSKLNLTGEIIANIYLGKITNWNDSLIQAINPDVTLPTKKITPVFRSDGSG
                     TTAIFANYLSKVSPTWKDTLGEGTALNFTVGVGGKGNPGVAGTVAVTDGAIGYIGSEY
                     AFSLGLSIASIQNSKGEFIQPNTASISAAAQGDIPADTRVMITNSEATGSYPISGFTW
                     IVVYKEQAYANRSIEKAEATVELLHFLLSEKAQSLTETVHYAPLSEAVIALTKANIKK
                     MTYQGNPIE"
     gene            1273..2256
                     /locus_tag="PLD76_00445"
     CDS             1273..2256
                     /locus_tag="PLD76_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26507.1"
                     /translation="MKKSILATFVFFAFLAVSHAQESVPFKMSAKPTITLFSNFKTGI
                     ADPNNKIGFNLERVYLGTSFVLSENLSGRVRFDIGNPNVADSDLEMVAYVKNAEIKWQ
                     QGDFTLNAGMISTIQFAVQEKFWGYRYLLKSFQDEYKFGSSADLGIAASYKISDLITI
                     DATVINGEGYKKVNKDDALRYGGGVLISPSEWILLRAYYDRNDVATGAIGATQENVGL
                     FAGISLQNITLGAEYNTLYNVDRTRGNHQYGYSVYSTVVLPKQFQLYGRWDYLFSSNE
                     YNLAKDGQLGVLGVQYSINKYVQVSPNLRIWQSAEGGAAKLCGLVNLQVNF"
     gene            complement(2325..2648)
                     /locus_tag="PLD76_00450"
     CDS             complement(2325..2648)
                     /locus_tag="PLD76_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU26508.1"
                     /translation="MTKHIFSPKRYLSSFLFVFFFFLSIVISFAQSITGTVIDKKTNE
                     TLAEAAIAVNGQKVYTDLDGNFTLANLGGSKCELTVSMISYETQTIEMDLAKNKDVAI
                     VLKKR"
     gene            complement(2814..5621)
                     /locus_tag="PLD76_00455"
     CDS             complement(2814..5621)
                     /locus_tag="PLD76_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU26509.1"
                     /translation="MQFKRVLTCIIGLSIMLIFSQCSTKNNTWLSRNFQSFSTRYNVY
                     FNANESYKKGIEKIASGHKDSYTEILPIFPISVHTNASFATNEMAISIEKSEKAIKNH
                     SIKRKPKRNPSKLQDPSYQAFLNQSEYNPMVEQSWLLIGKSQFHQADFMAANATFLYI
                     IRNYIHNHELVTAATIWLARSYGEVGWFYEAEDLMNKLDEGGFSPTTNLLFVYVKADL
                     LLKQKRYQEAVPFLESAYNAEKKQQQQARLGFILGQIYEKEQQYDKAFTYYNYVLKKN
                     PPYEMAFNANLKKTQSYQGKDFEKMIKDVTKLASNERNYEYLDQLYTAIAKLYQRNGK
                     TTKAIEYYQLAIEKSTRNGMDKAEALLSLGQLYINNKEYVKAQPLINEAVPILPSSYS
                     NYRSWRTISENLNELALHHNRIVLEDSLQKLAQLPEIERIKRIQEVIKVEQEAQEKLK
                     EKIDKEAKQQEERERNAFSTLPSLALGEIGDKSWYFYNPTLIGRGKIEFQRTWGNRIL
                     EDNWRRKNKTAVLNDRQETIDEVVQKIETSSTTENVTQQPDSISPTDKQIAKYLQQIP
                     LSVAQKENSDNAIMESLYNLVYIYKEKVPDYELAQQTYAELIERFPSSSFIAEATFNM
                     FQLHSKEQNLLEAERSKQYILANFPTSTYAVFLQNPEELNTLTQLRKEEEKLYAETYE
                     AFLKNDYAIVALNLEKAQKEFPFSKLTPKFRFLEALSTSKKKGKDAFKTSLELIVKDY
                     PDSEITPMAKDMIALMQQGQEPQSSLSHSNLLAERTSVVTEQVFAENIAKAGFVYEPQ
                     DNHLFVLIVNTDSITRNTLIFNIASYNFTRFLIKELDIQVKELDDVSVAIAISGLDNL
                     SEAIWYQKTLLADSTVTKQLQKTDYKGFVISEYNFSSVTNTESLERYFEFYRTNNLQI
                     TEPNTIKQLEQESGFVKGNAE"
     gene            5854..6798
                     /gene="mdh"
                     /locus_tag="PLD76_00460"
     CDS             5854..6798
                     /gene="mdh"
                     /locus_tag="PLD76_00460"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOU26510.1"
                     /translation="MKSKVTVVGAGNVGATCANVLAINEVASEVVLLDVKEGVAEGKA
                     MDIMQTAQLMGFDTVVKGCTNDYAQTANSDVVVITSGVPRKPGMTREDLIGINAGIVK
                     SVAESILAYSPNAILIIVSNPMDTMACLAMKILGLPSNRIIGMGGALDSSRFTYYLSQ
                     ALQCNANDVQGMVIGGHGDTTMVPLTSLATYKGVPVSKLLSKEKLQEIAKATMVGGAT
                     LTGLLGTSAWMAPGAASAIVVEAILRDQKKIIPCSVVLNGEYGLNDIMIGVPVILGKN
                     GVEKIVELELSEEEKEKFAASAVAVRKTNEILKEMNLV"
     gene            7061..8194
                     /locus_tag="PLD76_00465"
     CDS             7061..8194
                     /locus_tag="PLD76_00465"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOU26511.1"
                     /translation="MILGLLKEHGDETRVALLPDNVQQLVNQHVEVLVESGAGNLAFV
                     SDNDYISAGAKIVDRKTVFEKSSILLQIQVPLAEDVKKIQPSQVWISAFNPLWETALV
                     KTFQEKQITTFSLDSIPRSTRAQAMDILSSQATVTGYKAVLDAALISPTFFPMFMTAA
                     GTIRPANVLILGAGVAGLQAIATARKLGAQVEAFDVRTAVREEVKSLGARFVEVEGAV
                     EDKAAGGYAVEQTEEYKQKQQQAINDHAAKANIVICTAQIPGRKAPLLLPTEAVERMK
                     PGSVIIDLAASTGGNCELTKNNETIYHKGVIIVGQSNYPAKMPLDASRMLGKNIMNFL
                     ALLITKEGELHVNFDDDIVKGTCVTHQGEIVNERVKAVIGTIK"
     gene            8203..8529
                     /locus_tag="PLD76_00470"
     CDS             8203..8529
                     /locus_tag="PLD76_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOU26512.1"
                     /translation="MEQLLSLFFDYKEAIFIIALSVFLGIEVISNVPAVLHTPLMSGA
                     NAISGVIIIGGIILVGHADLSQFSVELILGVLALFFGTLNVVGGFAVTDRMLEMFKKK
                     KPTKTN"
     gene            8532..>9207
                     /locus_tag="PLD76_00475"
     CDS             8532..>9207
                     /locus_tag="PLD76_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOU26513.1"
                     /translation="MESTILQSIPSALATGDILLELSYLVSALLFVFGLKKLSHPETA
                     RNGNLWAAAGMLLAMVTTALLHKNADGETIKLTNLLVIVLAILVGAVVGWIMSHRVKM
                     TAMPQLVSLFNATGGAASALVALLEYPNAVAGNYGSIAVTILGLIIGAVAFSGSIIAY
                     GKLDGKVKDLRLSALTYINLLMLVAVVVVSVLLVISTQPPSDMMWAIWVLFAIALVYG
                     VLFVMPI"
BASE COUNT         2772 a         1483 c         1840 g         3112 t
ORIGIN      
        1 ataaaagcgt tttaaaggct ttatcagaca tgtcgttgta ttgttagttt attattctat
       61 tggattacct tggtaggtca tctttttaat atttgctttt gttaaagcaa ttacagcctc
      121 tgaaaggggg gcataatgaa cagtttctgt aagtgattga gctttttcgc tcaacaagaa
      181 atgtaacaac tcaacagttg cttctgcttt ctctatacta cgattggcat aagcttgttc
      241 tttatataca actatccaag tgaatccgct aataggatac gaacctgtgg cttctgagtt
      301 tgttatcata acgcgcgtgt cagcaggtat atcgccttgt gcagcagctg aaatagatgc
      361 tgtatttggt tgtataaatt ctccttttga gttttgaata gatgctatag ataagcccaa
      421 tgaaaaagca tattccgaac caatataacc aattgctcca tctgttacag ctactgttcc
      481 cgcaactcct ggattccctt ttccacctac tcctacagtg aaattaagag cagtaccttc
      541 acccagtgta tctttccatg tagggctaac tttgcttaaa taatttgcaa aaatagcggt
      601 tgttccacta ccatctgaac ggaatacggg agtaattttt ttagtaggta atgttacatc
      661 aggatttatg gcttgtatca atgaatcatt ccagtttgta atttttccta agtagatatt
      721 ggcaataatt tctcctgtaa gatttaattt tgagacgcct tctaaattat atgataacac
      781 cacagcaccc atacatgtag gtatgtgtat tacttcgtca atttcagcca ttctttcgtc
      841 gttcaaaaat gcatctgttc cagcgaagtc aacgattcct tcggtaatac tgcgaattcc
      901 accgccacta ccaattcctc cgtatgatac aatatttcct gtttctgctt tgtaggcttc
      961 aaaggctaca ttataatatg gaacaggaaa ggttgctcca gctcctgcaa gctctactgt
     1021 ttcggtgtta cagctgctta acaaaactgt aacagtgctc aataaaaata ctagtttttt
     1081 catgttttct gttgctaaat gtttaattaa atatgaataa aaagtttaca atgcaaagaa
     1141 acaactaact tgttgcggaa atgttacgca aatattacga agatgttaag atgtgaaagg
     1201 atagaatggt gcgtgtaaca agataaaaaa actttatata cttttgccta cgaaataaat
     1261 aattatcagg atatgaaaaa atcaatttta gctacattcg tattttttgc ttttttagca
     1321 gtgtctcatg cacaagagtc tgtccctttt aaaatgagtg caaaacctac cattactcta
     1381 tttagtaatt ttaaaacggg aatagcagat ccgaataata aaataggttt taatttagaa
     1441 agagtttatt tgggcacatc ctttgtatta agtgaaaact tgtcagggcg agtacggttt
     1501 gatatcggaa atccgaacgt agcggattct gacttagaaa tggtggcgta tgttaaaaat
     1561 gcagagatta aatggcaaca aggtgatttt actctgaatg caggtatgat tagcactatt
     1621 caatttgctg ttcaagaaaa gttttggggt taccgctatt tacttaaatc gtttcaagat
     1681 gagtataaat ttggttccag tgctgatttg gggattgctg caagttacaa aatatcagat
     1741 ttgataacga tagatgcaac agttattaat ggagaaggtt acaaaaaagt aaacaaagat
     1801 gatgcattgc gttacggagg aggagtactt atttcacctt cagaatggat tttgttgcgt
     1861 gcgtattatg atagaaatga tgtagctact ggagctatag gagccacaca agaaaatgta
     1921 ggtttgtttg caggaattag cttacaaaat atcacactag gagccgaata taatacattg
     1981 tataatgtgg atagaacaag aggcaatcat caatatggct attcggtata tagtacagtt
     2041 gtattaccaa aacaatttca gctttatggt cgttgggatt acttattttc gagtaatgag
     2101 tataacctag caaaagacgg tcagttagga gtattgggtg ttcaatatag tataaataaa
     2161 tatgtccaag tttcgccaaa tcttagaata tggcaatcag ctgaaggtgg agcggctaag
     2221 ctttgtggtt tggttaattt gcaggttaac ttttaggtaa aaaggataaa gagcgatgaa
     2281 aaaatttttt catcgctctt tatcctttta tcaatcaata ccctttatct ttttttcaat
     2341 acaatagcta catctttatt tttagccaaa tccatttcga tggtttgtgt ttcgtacgaa
     2401 atcatactca ctgtaagttc gcatttgcta ccacctaggt ttgctagggt aaaattgcca
     2461 tctaagtcgg tgtatacttt ttgaccattt accgcaattg ctgcttctgc aagagtttcg
     2521 tttgtttttt tatcaataac agttccagta atactttgag caaaacttat gactatggat
     2581 aaaaaaaaga aaaaaacaaa taggaatgat gataggtatc gtttcggcga aaaaatgtgt
     2641 tttgtcatat tgcagttaag tttaggtata ggtcgttttt gataaacgaa tctattttac
     2701 aaccgcaaag gtaaaaactg ggtgttgcgt tcttgttacg gaatgattac ggacttgtga
     2761 cgattttgtt cggggaaaaa ccagctcttt tacatgtcga atttgctgaa ctattattcg
     2821 gcgttacctt ttacaaaccc cgattcttgc tctaattgct tgatggtatt aggctctgta
     2881 atttgtaggt tattggtacg ataaaattca aaataacgtt ccaatgattc tgtatttgta
     2941 acagaagaaa aattatattc agaaattaca aaacctttgt aatcggtttt ttgtaattgt
     3001 tttgtaacag tactatctgc taacagtgtt ttttggtacc atatagcttc agataaatta
     3061 tctaatccac taatggcaat tgcgacactt acatcgtcta attcttttac ttgaatatcc
     3121 aattctttaa ttaaaaaacg agtaaagtta tacgatgcta tattaaaaat aagagtattt
     3181 cgggtaattg aatctgtgtt aactattaat acaaataaat ggttgtcctg tggttcgtat
     3241 acaaaacctg ctttggcaat gttttctgca aatacctgtt ccgttactac gctagtgcgt
     3301 tcggcgagca agttgctgtg cgaaagagag gattggggct cttgtccttg ttgcattagg
     3361 gctatcatat cttttgccat tggtgtaatt tcactgtcag gatagtcctt tacgataagt
     3421 tcaaggcttg ttttaaaagc atctttccct ttctttttac tagtgcttaa tgcctctaaa
     3481 aagcgaaact ttggcgtaag tttagaaaaa ggaaattctt tttgtgcttt ttccaagttt
     3541 aaggcaacta tagcataatc attcttcaga aatgcttcgt atgtttcagc atagagtttt
     3601 tcttcttctt ttcgtaattg tgttagggtg ttgagctcct ctggattttg caaaaaaaca
     3661 gcataggttg aagttgggaa attcgctaaa atatattgtt tgcttctttc tgcctctaac
     3721 aagttttgtt ctttactatg taattgaaac atattaaatg ttgcttcggc aatgaaagag
     3781 gacgaaggaa atcgttcgat taattcagca tatgtttgtt gagccaattc ataatcaggt
     3841 actttttctt tgtatatgta cacaagattg tataaacttt ccataatggc attatcagaa
     3901 ttttcttttt gtgccactga aagcggtatc tgttgtaaat acttcgctat ttgtttgtct
     3961 gtaggtgata tggagtctgg ttgttgtgtt acattttctg ttgttgacga ggtttctatc
     4021 ttttgaacaa cttcatctat tgtttcttgc ctatcattta atactgctgt tttgtttttt
     4081 cttcgccagt tatcctctag tatcctattt ccccaggtac gttgaaattc tatttttcct
     4141 cttccgatta atgtggggtt gtaaaaatac catgatttat ctccaatttc ccctaaagcg
     4201 agggagggaa gagtagaaaa tgcattgcgt tcgcgttctt cttgttgttt tgcctctttg
     4261 tcaatctttt cttttaattt ttcttgagct tcttgttcta cttttattac ttcttgtatc
     4321 cgttttatgc gttcaatttc aggtaattgt gccaattttt ggagactgtc ttctagcact
     4381 attcgattgt ggtgtaaagc aagttcgttc agattttcgg atattgtacg ccacgaacga
     4441 tagttagaat atgaggaagg caagataggt acagcctcat ttatcaaagg ttgtgctttt
     4501 acgtattctt tgttgttgat gtatagctgt ccaagagaca aaagagcttc tgctttatcc
     4561 atgccattgc gtgtgctttt ttctattgca agttggtagt attctattgc cttagtcgtt
     4621 tttccgtttc gttgataaag ttttgctatt gccgtatata gctgatctaa gtattcataa
     4681 ttacgttcat tggatgctaa tttagttaca tctttaatca ttttttcgaa atcttttcct
     4741 tggtacgatt gagttttctt caagttggca ttaaacgcca tttcatacgg aggatttttt
     4801 ttcagtacgt agttgtaata cgtaaatgct ttgtcatatt gctgttcttt ttcgtaaatt
     4861 tgtcccaaga taaatcctaa gcgagcttgt tgctgttgtt ttttttcagc attataagct
     4921 gattctaaaa aaggcacggc ttcttggtat cgtttttgtt tgagtagtaa atccgctttt
     4981 acatacacaa acaataaatt tgttgtcgga ctaaaccctc cttcgtcaag cttattcatc
     5041 aggtcttctg cctcataaaa ccaaccgact tcaccatagc tacgagccaa ccatattgtt
     5101 gctgctgtaa ccaattcatg gttatgaata taattacgga taatatacaa aaaagtggca
     5161 ttagctgcca taaaatcagc ttgatggaat tgtgattttc caattaaaag ccaagattgt
     5221 tccaccattg gattgtattc tgattgattc agaaaagcct gataggatgg gtcttgtaat
     5281 ttcgatggat tgcgttttgg cttgcgtttt atggagtggt tttttatggc tttttcgctt
     5341 ttttctatgc tgatagccat ttcgttggta gcaaaagagg catttgtatg cacgctgatt
     5401 ggaaaaatgg gcaatatttc ggtgtaggaa tctttgtgtc ccgaagcaat tttttctatt
     5461 ccctttttat agctttcatt ggcattgaaa taaacattat agcgtgtgga aaaagactga
     5521 aaattacgag acaaccatgt gttgtttttt gttgaacact ggctaaaaat aagcataatc
     5581 gacaacccga ttatacaggt aagcactcgt ttaaattgca tagtatatcc cattttcggt
     5641 acaaaagtac acaaattatg ctagtatgct atgaaaaata gcttcgtttt ttgtaaattt
     5701 aagtgttcct gtcttttatt gttgaaaaca gggtgtaaaa aatgttattt ttttgttaag
     5761 gaagtgctga tttattctct tctttctttg atatgaaaga aaaaattgta attttgtggg
     5821 cagtaaaatc accactttta aaaaatacaa ataatgaagt cgaaagtaac cgttgtagga
     5881 gcaggaaatg taggggcaac ctgtgctaat gtattagcaa ttaatgaagt agcaagcgaa
     5941 gtcgttttgc tagatgtgaa agagggtgtg gctgaaggga aagccatgga tattatgcaa
     6001 acagcccaac taatgggttt tgatacggtg gtaaaaggat gtaccaacga ttatgctcaa
     6061 actgccaatt cggatgtcgt ggtaattacg tcgggagtac cacgtaaacc aggtatgaca
     6121 agagaagatt tgataggtat taacgctggt atagtaaaaa gtgtggcaga aagtattttg
     6181 gcatattcgc ctaatgcaat tttaattatc gtttccaacc caatggatac gatggcatgt
     6241 ttggctatga aaatattagg attaccttca aacagaatta ttggtatggg aggagcatta
     6301 gatagttcac gttttacgta ttatttgagc caagcattac aatgcaatgc caacgatgtg
     6361 caagggatgg taattggcgg acatggcgac actacaatgg tgccacttac cagtttagct
     6421 acgtataaag gcgttccagt aagcaaattg ttaagcaaag aaaaattaca agaaatagca
     6481 aaagcaacaa tggtaggtgg agctacacta acaggcttat taggaacatc agcatggatg
     6541 gctcctggtg cagcatcggc tattgtggtg gaagctatat tacgtgacca aaagaaaatt
     6601 attccttgtt cggttgtttt aaatggtgaa tacggcttaa acgatattat gattggtgtg
     6661 cctgtaatct taggaaaaaa tggggttgaa aaaattgttg aacttgaatt aagcgaagaa
     6721 gaaaaagaaa aatttgctgc cagtgcagta gctgttcgca aaaccaacga aattttaaag
     6781 gaaatgaatt tggtgtaaaa ccatcatcat ttgctatctt atacaagaac agtctcagaa
     6841 cggggctgtt cttttttatt acataaatgc aacaaaaaat actctttcgt cttacaataa
     6901 acaatcaata cccctacatt tagttataaa ttcgcatata ttaacatctg tttagcgttt
     6961 cttgtgaaaa ttacaatagt gatagcaaaa taatatactt atttttgtcg gcatattctg
     7021 agtagaaaaa ataaattcat taacgcaaaa aaagtaaatt atgattctgg gtcttttaaa
     7081 agaacatgga gatgaaacca gggtagcttt acttcccgat aatgttcaac agttggtaaa
     7141 ccaacacgta gaagtattag ttgaaagtgg agcaggcaat ctggcttttg tttctgataa
     7201 tgattacata tcagctgggg caaaaatagt agataggaaa acggtatttg aaaaatcatc
     7261 aatattattg caaatacaag taccgctagc tgaggacgta aaaaaaattc aaccaagcca
     7321 agtatggata agtgcattca atccattatg ggaaacggca ctagtaaaaa catttcaaga
     7381 aaaacaaatt accaccttta gtttagactc aatacctcgc tctacgcggg cacaagccat
     7441 ggacatattg tcgtctcagg ctacggtaac tggctacaag gctgtacttg acgctgcttt
     7501 aatttcgccc acatttttcc ctatgtttat gacagcagca ggcactattc gacctgccaa
     7561 cgtattgata ttaggtgctg gagtagctgg tttacaagcc attgccactg cacgaaagtt
     7621 aggtgctcaa gttgaagcat ttgatgtgcg aacagctgta cgtgaagaag tgaaaagttt
     7681 gggagcacgt tttgttgaag tagaaggtgc ggtagaagat aaagctgcag gaggctatgc
     7741 cgtagaacaa accgaagaat acaagcaaaa acaacaacaa gcaattaacg accatgcagc
     7801 taaagcaaat attgtaattt gtacagcgca aataccaggc agaaaagccc cgttattatt
     7861 accaacagag gcagtagaaa gaatgaagcc cggctcggta attatcgatt tggcagcttc
     7921 tacaggtggc aattgtgagt tgacaaaaaa caacgaaact atttatcaca aaggcgtaat
     7981 tattgtagga caatcaaatt atccagctaa aatgccattg gatgcaagtc gtatgttagg
     8041 aaaaaatatc atgaattttt tggctttact tattacaaaa gaaggagagt tgcatgttaa
     8101 ttttgacgat gatatcgtga aaggtacttg tgtaacacac caaggagaga ttgtaaacga
     8161 aagagtaaaa gctgtaattg gaactataaa ataaacactg ttatggaaca actattatct
     8221 ttattttttg actataaaga agcaatcttt atcattgctt tgtcggtttt tttgggcatt
     8281 gaggttattt cgaatgtgcc tgctgtatta cacacaccat taatgtcggg tgcaaatgcc
     8341 atatctggcg ttatcatcat tggaggtatt attcttgtgg gacacgctga tttgtcgcaa
     8401 ttctcagtag agttgatact cggtgttttg gccttgtttt ttggcacatt gaatgtggta
     8461 ggaggctttg cagtaactga tagaatgctc gaaatgttta aaaagaaaaa acctactaaa
     8521 acgaattaat tatggaatct actatattac aatctattcc gtcagcattg gctacgggag
     8581 acattttatt agaactgagc tatctggtat ctgctttgtt atttgtgttt ggattgaaaa
     8641 aattgtcgca cccagaaact gccagaaatg gaaacttatg ggcagcagca ggtatgcttt
     8701 tggcaatggt tacaacggca ttgttgcata agaatgcaga tggcgaaacc ataaaattga
     8761 ccaatttatt ggtgattgtt ttagcaatac ttgttggagc agttgtaggt tggattatgt
     8821 ctcatcgtgt taagatgacg gcgatgccac agcttgtttc tttgtttaac gccacaggtg
     8881 gagcagcctc tgcattggta gccctgctcg aatatcccaa tgcagtagcg ggaaattatg
     8941 gttctatagc tgttactata ttggggttaa ttattggagc agttgctttt agtgggagta
     9001 ttattgctta tggcaagtta gatggaaagg taaaagacct tcgtttatct gctctcactt
     9061 atataaattt attaatgttg gtagccgttg tagtggtgtc agtgttatta gttatttcta
     9121 cacagccacc atccgacatg atgtgggcaa tatgggtatt gtttgcaatc gcattggtgt
     9181 atggcgtttt gtttgttatg ccaattg
//
LOCUS       DAOSMY010000009         9009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_7696_length_9009_cov_3.656466, whole genome shotgun sequence.
ACCESSION   DAOSMY010000009 DAOSMY010000000
VERSION     DAOSMY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7696_length_9009_cov_3.656466"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>642)
                     /locus_tag="PLD76_00480"
     CDS             complement(<1..>642)
                     /locus_tag="PLD76_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26514.1"
                     /translation="EVSQGQTTIRQASVSMKMKVDENLLPLILRNKFAISSSAVSGDS
                     GVYDHVLTYKVGSTAGSGESFTLFWDDPDIGDCVISGFRFNAMNFEYKPQDFVMVSTE
                     GVGKYFTETAVTNTVSATLREFVGRHTKTEIADYGGAYAEESLYELAQNHSFEGAGED
                     RNFYLGSADMAVNLLKQSKFELSALVKYTDLTYRNDYENRTQKALKVTVTDTGR"
     assembly_gap    643..652
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(802..963)
                     /locus_tag="PLD76_00485"
     CDS             complement(802..963)
                     /locus_tag="PLD76_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26515.1"
                     /translation="MKRKQVKDKIVEVVKKTTFTRWINNRKVVVEAISLAEAEVLFKK
                     AEKELNLHN"
     gene            complement(1040..1459)
                     /locus_tag="PLD76_00490"
     CDS             complement(1040..1459)
                     /locus_tag="PLD76_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26516.1"
                     /translation="MKHYDGSNSVFNDVQRIYLDFPELLPSAQIVPIGLNIDSEMIDF
                     DNRELKFALIVYENIEADATQATIDLKVDRLCDIEDVIARYIEQVPNNVGIVSGVRIY
                     KTTVSNGRWTFEQGNNGMMLYLVIEFSCFTQLTVKDL"
     gene            complement(1500..1874)
                     /locus_tag="PLD76_00495"
     CDS             complement(1500..1874)
                     /locus_tag="PLD76_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26517.1"
                     /translation="MLVEREYKIEAPVNDGLLRNSIKTVQKSELEYTITTTAQQGGFP
                     YPIAVHEGTGKYKGTSDRGRGNWSRMAGYTPEDIAFFKMLAKRRAISQKPNKFAERAE
                     KTAKPNVIKFISRELNNELHKD"
     gene            complement(1958..2311)
                     /locus_tag="PLD76_00500"
     CDS             complement(1958..2311)
                     /locus_tag="PLD76_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26518.1"
                     /translation="MSFIEEFWNSPTYALFSVKRATTSTGSRIDTQSTVSSGNKGIVR
                     VPSVQSQTLTEDEWGKEYHLYCNPNIDIKEGDKITIETGSTSAYTGTYSVRGVGIQED
                     LVDDDSFIKIILYKK"
     gene            complement(2308..2895)
                     /locus_tag="PLD76_00505"
     CDS             complement(2308..2895)
                     /locus_tag="PLD76_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HOU26519.1"
                     /translation="MISLSEAKAFMGVTSSTDDTLIGTLIDYAEGLIENYIDNKIDQV
                     SISNEILEIVDRFDLQYIPSFDLAPKYKYARTLYYPIASLSITQDGTTLTSGTDYTYE
                     ANTGVITFYTNVSDYQQGLKANYTAGYSTCPNDIKLVALELTKALYQNAGATAKGYGS
                     VSNKKIGDFSVSYKSTNVDITAWSGVLDKYRGISL"
     gene            complement(2892..3161)
                     /locus_tag="PLD76_00510"
     CDS             complement(2892..3161)
                     /locus_tag="PLD76_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26520.1"
                     /translation="MLVSAKWQIAYKNVYHRAGDVFEMADGDYGQYKNDIVIVNDGVW
                     KKPVVKQETSVDTYQNREKFEEETGKKAFWNGVLTKSYLSWLENK"
     gene            complement(3227..4408)
                     /locus_tag="PLD76_00515"
     CDS             complement(3227..4408)
                     /locus_tag="PLD76_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOU26521.1"
                     /translation="MADGIKKVEAVEKTVEADALMTKTEFKEIVEEIVAKQAESVKAR
                     EKKFELTGNKEDEVKLVETKKRNETVEFLNAWSNRDVKSLKKMHESRAKALNEGTGSA
                     GGYLVPEEFERTIERLMNDYSVIRQNATRIPMSTDVKRLNSLTGNPTVHIVGELTTIT
                     GSQNTFAEPVLTAKKYACITDWSNEVFEDSEVSLIQQIQETIAEEMAQAEENEFINGT
                     TSGSEGLMVVSGVTGNSLISGTTFANITFDDLSAMIVSLENVSNVSNRSAAFYMSPYV
                     YGQLRIKKGTDNHYILPPVPSSDMPASAFGKPIYTCLSMPSSTATGTKFVFYTDLRKT
                     AVIGDRRGMTVKVFDSGSVTESDGTTRNLITQDAEALRVTKRTAFCTRLQTGIVTLAT
                     N"
     gene            complement(4437..5333)
                     /locus_tag="PLD76_00520"
     CDS             complement(4437..5333)
                     /locus_tag="PLD76_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HOU26522.1"
                     /translation="MPIPIPKPEQSKEDFMKVSEGMPQGQASAVCIAQWDKKGDKSKD
                     DKPQKKSLINKAFFSAEVKQVDKEIEVTLSKQIRDRDGEIVDIKGGDLRNFSKNPIML
                     WGHRMIYGDVEDVMGHWENLRKTNDKILANPVFAEHPKAQYLKRMVEDGHVRSVSISF
                     IVNDGGFNLESKTVKSWEMLEASWVTVPSNVEAVSNVKEFKKLDDELTEKQYRQLLNY
                     KEIHPKIKQYRKLFMSEEFCGVLGFEKTGNELVDLKSIYDLIVSKLKETPKAEVKQET
                     PAYVTRKDAIKMIEELVKSGII"
     gene            complement(5391..7460)
                     /locus_tag="PLD76_00525"
     CDS             complement(5391..7460)
                     /locus_tag="PLD76_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOU26523.1"
                     /translation="MSILDVLKKETKSPEFSVSTQYQWDDYSDLVDSQNYFDQYFGWT
                     YKAIKTVADAVSSYDIPLYRKFGEQEIELDKKFPLVNDLLHFNDYMTLRQARALTITH
                     RRLSGLAFWYIVDSDNPNNRYDFYILDPTRVSVVVNNFGLPEYYKYRDANGQYHNIDR
                     EDIIEFKDPDPKNWIRGKGVLQATRYPHNTWELASKFNMNFFGNNAKPEGFLVLDGIS
                     RNEKKRIENELKDKYGGVDNAGKLGILNRMAQFIGITSSQKDLDFTNGLVTMREEILA
                     IHGVPKPLVGLTDSTFSNSLEAQRIFQRYTVKPELLAEEDVYNEQLIKKYFDTSRSVD
                     IYFKSPDPVEADEQVDSNVAVALYQGGIITLNEAREKVGYGTMDDGDELKKPQQLPTM
                     ALQNTNDGNNENMPEKDSDDNEKSLKGIKNELKRVQMSIKSIPSVKEAMLSKRVQLKQ
                     FFADKFDGFEDRFVKIAQEYFDVQEKRVTQEVFPKSKKAISLDFEFDYKNEVQIAIDT
                     FAPIFNDVAYDANLIANELAGGNVVIDEAGRKLIDKNLDKFAKEINKTTKDKITELVA
                     DGIEKQLSVEEIKNVIAEQFNRWAYGDGVIETRAKAMARTSVTGVFNQMSRRNYAENE
                     YVKGFEWLSAHDRFVRDAHAEADGQQVVKGQKFNVGGEYLTAPGDPSGSAGNVINCRC
                     TIIPVII"
     gene            complement(7489..8865)
                     /locus_tag="PLD76_00530"
     CDS             complement(7489..8865)
                     /locus_tag="PLD76_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26524.1"
                     /translation="MSKLKRTERNDELSEIGITVAKRYFDVDLYYTQGLILGAILDPD
                     IKEVCIITPTRYGKTFTIGMGALVGASLYDWEVQIGASDKEKSKIAMTKVMRMLPTAD
                     KCIAQNLIGGDTLTKLSQTLSKNKLGWSSGGSIDIFSVSERLKNTDISGQGAIGLGGD
                     LIILDESALISDENYATVRRMLAESSHTKLVEISNPHRKRHFYDSMNNPEVFTVWIDT
                     DDAIEEGRFNIEDVQRAKRGMGKRAIKVFFDCEFVDELENGFFTEDLIASTLSVPILN
                     GGIRVMGIDVARSGGDRIVYAIGRRRGDRFTIDDYIIENTGSAKIPVTHVAGKALAIA
                     KDLKIVYIGVDVLGLGAGVFDILDENGEFEVEEFIASAQADNKKTYANRKAEVADNFR
                     QDMGAGFVSLPKHDGLYRDLSGYAEEFDSQGRVKLADPADSPDIGDATLIAYSIAKEP
                     RDAELTFL"
BASE COUNT         2363 a         2183 c         1245 g         3208 t
ORIGIN      
        1 tctccccgtg tcggttacag ttacctttaa cgccttttgt gttctgttct cataatcgtt
       61 cctgtatgtt aaatccgtat atttcactaa tgcactcaat tcaaacttgc tttgtttcaa
      121 tagattaact gccatgtcgg cacttcctaa gtagaagttc ctatcttcac ctgcaccttc
      181 aaacgaatga ttttgtgcta attcgtacaa cgactcctca gcataagctc caccgtaatc
      241 tgcaatctct gttttagtat gtcttcccac aaactctctt aacgttgcag atacagtatt
      301 tgttactgct gtttcagtaa aatattttcc aactccttct gttgatacca taacaaaatc
      361 ttgtggcttg tattcaaagt tcatagcatt aaatctaaac ccacttatta cacaatctcc
      421 tatatctgga tcgtcccaaa acaatgtaaa gctttcacca cttccagcag tagagccgac
      481 tttataggtc aacacgtggt cgtaaacacc actatctcca ctaacagcac tcgaactaat
      541 cgcaaactta tttctcaata ttaacggaag taagttttca tctactttca ttttcataga
      601 aactgacgcc tgcctaattg tagtctgtcc ttgagatact tcnnnnnnnn nncatgctag
      661 ggttttccac gtacatcacc tgagggtcgt aagacaactc cacgtttgga gttatatacc
      721 tagtacctgt tgaggcggtt aacggtgtac tctcctgtgc aatcgcaagg gtaacacgcc
      781 ttaaaattcc ttgtgccata attaattatg taaatttaac tctttttccg cctttttgaa
      841 caatacttcc gcctctgcta agctaattgc ttcaacaacc accttacgat tatttatcca
      901 tctggtaaat gttgtttttt ttacaacttc aacaatttta tctttaactt gtttcctttt
      961 cataatacta ctattcaaat aatttcgcaa ccacctattt ataacaggag tgaggggcgg
     1021 ttgtcctcac tccctacctc tagaggtctt taactgtcaa ttgcgtaaag caactgaact
     1081 ctattaccaa atataacatc ataccgttat tgccttgttc aaatgtccat cttccgttgc
     1141 taaccgttgt cttgtatatc ctaactcctg atactatacc tacattgttc ggtacttgtt
     1201 ctatgtatct cgctataaca tcttcaatat cgcataatct atcgactttt aaatctattg
     1261 tagcctgtgt tgcatcggct tctatattct catatacaat taacgcaaac ttcaattccc
     1321 tattgtcaaa atctatcatc tcactatcaa tgtttaaccc aataggtact atctgtgcag
     1381 atggtaacaa ctcaggaaag tctaaatata tacgctgtac atcgttgaac acactattgc
     1441 taccgtcgta atgtttcaac gccttaatct ctgttactaa attgtctaat actgtttttc
     1501 taatccttgt gaagctcatt atttaactcc cttgaaataa acttaatcac attcggcttg
     1561 gctgtctttt cggctctttc cgcaaactta ttaggtttct gtgaaattgc ccttctttta
     1621 gccaacatct taaagaaagc aatgtcctct ggggtataac cagccattct tgaccaattt
     1681 ccacgacccc tatctgacgt tcctttatat tttccagttc cttcgtgtac cgctattgga
     1741 taaggaaatc caccctgctg tgctgtggtt gtaattgtat attctagttc gctcttttgt
     1801 accgttttaa tactgttcct caacaaccca tcgttaacag gtgcctcaat tttatattcc
     1861 ctttcgacaa gcatcgtact cgcctgtaaa atctttccca aattaacttt gcccgttctg
     1921 atcgcttgcc ctaatttctt ttgtacacta acttctatca tttcttgtac aatataattt
     1981 tgataaaact atcgtcatca accaaatcct cttgtattcc tactcctcgt actgaatacg
     2041 tccctgtata tgcacttgtg cttcccgttt ctatcgtaat tttatccccc tctttaatgt
     2101 caatgttcgg attacagtac aaatgatatt ctttgcccca ttcgtcctct gtaagtgttt
     2161 gagattgtac actaggaaca cgcacaatgc ccttatttcc gcttgaaact gtactctggg
     2221 tatcaatcct actccccgtg cttgttgtgg ctctttttac gctaaataaa gcgtatgtag
     2281 gactgttcca aaactcctct ataaaactca taacgatata cccctatatt tatctaaaac
     2341 accgctccac gcagttatgt ctacattcgt tgacttatag cttacgctaa aatcgcctat
     2401 tttcttgtta gaaacgcttc catatccctt cgccgttgct ccagcatttt gatataaagc
     2461 ctttgtcaat tctaaagcca ctaatttaat atcgtttgga catgtagaat accctgcagt
     2521 atagtttgcc tttaatcctt gctgataatc agatacgttc gtgtagaaag taatcactcc
     2581 agtattagcc tcgtaagtat aatcagttcc acttgtcaat gtggtaccgt cctgcgtaat
     2641 actcaacgaa gctatcggat aatacaacgt tcttgcatat ttatatttag gagccaaatc
     2701 aaaactaggt atatactgca aatcaaatct gtcaacaatt tctaatatct catttgaaat
     2761 acttacttgg tcgattttat tatctatata attctctatt aatccttctg cgtagtctat
     2821 tagcgtacca attaaagtat cgtcagtact tgaagtaaca cccataaaag ccttagcttc
     2881 tgaaagactt atcatttatt ttccaaccaa cttaaatacg acttggttaa cacgccattc
     2941 caaaacgctt ttttgccagt ttcctcttca aatttctctc tattctgata tgtatctaca
     3001 gatgtttctt gtttgacaac tggttttttc catactccat catttacaat aacaatatca
     3061 tttttatatt gaccgtagtc gccatcggcc atctcgaaca catcaccagc acgatgatat
     3121 acgtttttat atgcaatttg ccactttgct gacaccaaca tttttataac cgctttaaat
     3181 taatttcacc acaagtagga gccgaaactc ccactctgtg gtaaaactag tttgtggcta
     3241 aagttacaat tcctgtttgt aatcttgtgc agaacgcagt cctcttagtc accctcaaag
     3301 cttccgcatc ttgagtaatt aaattccttg tagttccatc actctcggtt actgatcctg
     3361 aatcgaatac tttaacggtc attccccttc tatcaccaat aacagctgtc tttctcaagt
     3421 ctgtatagaa tacaaactta gttcctgttg ctgtagatga tggcatactc aagcaggtgt
     3481 aaattggctt tccaaatgca cttgctggca tatcgcttga tggtactgga ggtaatatat
     3541 agtggttatc tgtacccttt ttgattctca attgtccgta aacgtaagga ctcatgtaga
     3601 aagccgcact tcggttgcta acattagaaa cattctctaa cgatactatc attgctgata
     3661 agtcatcaaa ggtaatgttt gcaaacgttg ttccagagat taagctattt ccagttacac
     3721 cagaaacaac cattaagccc tcgctaccag aagtagtccc attaatgaac tcattctctt
     3781 cagcttgagc catttcctct gcaatagtct cctgaatctg ttgaatcaaa gaaacttcac
     3841 tgtcctcaaa tacttcgttt gaccagtctg taatacatgc atatttcttg gcagttaata
     3901 caggctctgc gaaggtattt tgacttcctg ttatggttgt caattctcca actatatgta
     3961 cagttggatt tccagtcaat gagttaagtc ttttgacatc tgttgacatt ggaattctgg
     4021 ttgcattttg tcgaataact gagtagtcat tcattaacct ttcaattgtt ctttcaaatt
     4081 cttcaggcac taagtaccca ccagctgaac cagttccttc gttcaatgcc tttgccctac
     4141 tctcgtgcat tttcttgagc gatttcacgt ccctgttgct ccatgcgttt aagaactcta
     4201 ctgtttcatt tctctttttt gtctctacta atttgacttc atcttctttg tttccggtta
     4261 actcaaattt cttttcacga gccttcacac tctctgcctg ttttgcaaca atttcttcga
     4321 ctatttcttt aaactccgtt ttagtcatta gagcatctgc ttctactgtt ttttcaacag
     4381 cttccacttt cttaattccg tctgccatga ttaaaataaa ctaaataaga ccccgcctaa
     4441 ataataccgc tttttactaa ctcttctatc attttaattg catctttacg tgttacgtat
     4501 gcaggggtct cctgcttaac ctcagcttta ggggtctcct tcagcttgga cacaattaaa
     4561 tcatatatac tttttaaatc tactaactcg ttaccagttt tttcaaatcc taacacccca
     4621 caaaactctt cactcatgaa taattttctg tactgcttta ttttaggatg tatttcctta
     4681 tagttcaata gttgtcggta ctgtttctcg gttagttcat cgtctagttt tttaaattcc
     4741 tttacgttac ttacagcctc aacgttggaa ggtacagtta cccaactggc ttctaacatt
     4801 tcccaactct ttaccgtctt actttctaaa ttaaatccac cgtcattaac tataaaacta
     4861 atactaacac ttcttacgtg tccgtcctct accattcttt ttaaatattg tgctttggga
     4921 tgttccgcaa atactggatt tgctaatatc ttatcgttag tttttcttaa attttcccaa
     4981 tgtcccatca catcttcaac gtcaccgtat atcattctat gcccccataa catgatagga
     5041 tttttactaa agttcctcaa atcaccacct ttaatatcta ctatctcgcc gtccctatct
     5101 ctaatctgtt tgctcaacgt aacctctatt tctttgtcta cttgcttaac ctcggcacta
     5161 aaaaaagcct tattgatcag acttttcttt tgtggtttat catctttaga cttatcgcct
     5221 ttcttgtccc attgtgcaat gcaaaccgca gaagcttggc cctgtggcat gccttcactt
     5281 actttcataa aatcttcttt agattgttct ggtttcggta ttggtattgg cattttctgt
     5341 aaaaatcaac ttatgtaaac aatgttatca ctaaacacgc caactcgcaa ctatataata
     5401 acaggtatta tcgtacacct gcaattaatg acatttccag cacttcccga tggatctcct
     5461 ggtgcggtta gatattcacc accaacatta aacttctgtc ccttcactac ttgctgtccg
     5521 tctgcttctg cgtgtgcatc cctcacaaac ctgtcatgag cagacaacca ctcaaatccc
     5581 tttacatatt cgttttctgc gtaatttctt ctactcatct gattaaacac tccggttacg
     5641 ctcgttcttg ccatagcctt cgccctagtc tcaatcacac cgtctccata cgcccatctg
     5701 ttgaactgtt ccgcaatcac gttcttaatt tcttctacag ataactgctt ctctatcccg
     5761 tcagcaacca gttcagtaat cttatccttt gtggttttgt ttatctcttt tgcgaacttg
     5821 tctaaattct tgtctatcaa ttttcttcca gcctcatcaa taacaacgtt accacctgcc
     5881 aactcgttag caattaggtt agcatcatat gcaacgtcat tgaatatcgg agcaaatgtg
     5941 tctatggcaa tttgtacctc gttcttataa tcaaactcaa aatcgagact aatagccttc
     6001 ttgctctttg ggaacacctc ctgtgttacc ctcttttctt gtacgtcaaa atattcttgt
     6061 gctattttta caaacctatc ttcaaatccg tcaaacttat ctgcaaaaaa ttgctttagt
     6121 tgcactctct tgcttaacat cgcttcttta acgcttggga tagacttaat actcatctgt
     6181 acccgtttta actcgttttt gataccttta agcgacttct cgttatcgtc actgtctttt
     6241 tctggcatgt tttcattatt accgtcattt gtgttttgaa gtgccatcgt tgggagctgt
     6301 tgaggcttct ttagttcgtc tccatcatcc atcgtcccgt agcctacctt ctctctggct
     6361 tcgttaagtg taataattcc tccttgatat aaagcgaccg caacgttgct gtccacttgt
     6421 tcgtctgctt caactggatc gggagactta aaatatatat caacactcct agaagtatca
     6481 aaatactttt taattaactg ttcgttgtac acgtcttctt ctgccaacag ctctggcttg
     6541 accgtatatc tctgaaatat tctttgtgct tctaaagagt tactaaacgt gctgtcggtt
     6601 aaccccacta atggctttgg tactccgtga attgccaata tctcttctcg catggtgact
     6661 agaccatttg tgaagtccaa atctttctgt gaacttgtta ttcctataaa ctgtgccatt
     6721 ctattaagta ttcccagctt tcctgcgtta tctacaccgc cgtatttgtc tttaagctcg
     6781 ttttctattc tcttcttttc gttcctgctt ataccgtcta aaactaaaaa gccctctggt
     6841 ttagcattgt ttccaaagaa gttcatgtta aatttactgg ctaactccca agtattatga
     6901 ggatatctgg tagcctgtaa tactcctttc cctctaatcc aattcttcgg gtcgggatct
     6961 ttaaattcaa ttatatcctc cctatctatg ttgtgatatt gcccatttgc atctctgtat
     7021 ttgtaatatt caggtaatcc aaaattattg actacaacgc taactctggt cgggtctaat
     7081 atataaaaat cgtacctgtt gttaggattg tcactatcta caatatacca gaatgctaat
     7141 ccagataatc tcctgtgggt aatggttaaa gccctcgctt gtctcaatgt catgtagtca
     7201 ttgaagtgca acaaatcatt aaccagtgga aacttcttgt ctaactctat ttcttgctca
     7261 ccaaactttc gatacagtgg tatatcatat gagcttacag cgtctgcaac ggtcttaata
     7321 gctttataag tccatccgaa atactggtca aaataattct gagagtctac caagtccgag
     7381 taatcatccc attgatattg cgtactaact gaaaactctg gagacttggt ctccttcttt
     7441 aaaacatcta aaatgctcat aaaaacttcc gtaagttaaa acaccgccct acaaaaacgt
     7501 taactctgca tctctcggct ccttggctat agaatatgct attaaagtag catctccaat
     7561 atcaggagag tcagcgggat cagccaactt aacacgcccc tgagagtcaa actcttctgc
     7621 gtaaccgctt aaatctctat ataatccatc gtgcttaggt aacgatacaa acccagcccc
     7681 catgtcttgt ctaaagttat ccgcaacctc tgcctttcta ttggcgtacg tcttcttgtt
     7741 gtcggcttgt gcggaagcta taaattcctc tacttcaaac tccccattct catctaatat
     7801 gtcaaatact ccagcaccta aacccaatac atctacacca atatatacta tttttaagtc
     7861 tttcgcaata gccaacgcct taccagccac gtgtgtaact ggtattttcg cacttcctgt
     7921 attttcaatt atataatcat ctattgtgaa tctgtccccc cttctcctac ctatagcata
     7981 tactatccta tcccctccac ttcttgcaac atctattccc ataactctaa tacctccatt
     8041 cagtattgga acacttaaag tactggcaat taaatcctca gtaaaaaatc cgttttctaa
     8101 ttcatctacg aactcgcaat caaaaaacac tttaatcgct ctttttccca tacctctttt
     8161 ggctctttgt acatcttcta tattaaatct accttcctca atagcgtcat ctgtatctat
     8221 ccatacagta aacacctctg gattattcat actgtcgtaa aaatgcctct tcctgtgtgg
     8281 attacttatc tctactaact tggtatgtga actttcagct aacattcttc taactgtagc
     8341 gtaattctca tcgcttatta atgcactttc gtctaatata attaaatcac ctcccaaacc
     8401 aatcgcaccc tgtccactaa tatctgtatt ctttaatctt tcactcacgc taaagatatc
     8461 tatactacct ccagaactcc aacctaactt atttttgctc agtgtctgcg aaagttttgt
     8521 taatgtatct ccacctatta ggttttgtgc gatacattta tccgcagtgg gtaacatcct
     8581 cattaccttt gtcatcgcta tttttgactt ctctttatca ctagcaccta tctgtacctc
     8641 ccaatcgtat agagaagcac caactaaagc acccatacct atcgtgaatg tcttaccgta
     8701 ccttgtaggc gtaattatac aaacctcctt tatatcaggg tcaagtatcg cacccaaaat
     8761 taaaccttgt gtatagtaca aatcaacatc gaaatacctt ttagcaactg ttatacctat
     8821 ttcagaaagt tcatcatttc tttctgttct ttttaatttg ctcatataat tctttaagtg
     8881 acttcttttc tacgtttaca tctatatctc catctagttt gatgtcttgc ttcttgctaa
     8941 acccgccata ctttatttgc aataattcca tatatctaaa gtcactgctc gcttttaggt
     9001 aagatatcc
//
LOCUS       DAOSMY010000010         8967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_7748_length_8967_cov_4.066427, whole genome shotgun sequence.
ACCESSION   DAOSMY010000010 DAOSMY010000000
VERSION     DAOSMY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7748_length_8967_cov_4.066427"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLD76_00535"
     CDS             <1..812
                     /locus_tag="PLD76_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOU26525.1"
                     /translation="FLVLNTSGLTIIPISIMVYRAQMGAADPSDIFIPILLSTFVSTL
                     AGIIAVSIAQKINLFTKTILLYLGSITLLLTGVIWGFTVLSSEKLNMVSSVAANIILF
                     SIIIGFILLAIRKKINVYDSFIEGAKDGFSIAVKIIPFLVAILVGIGVFRASGAMDSL
                     MSGIAWLFTSIGLNANFVEALPVAFMKPLSGAGARGLMIDAMNQHGVDSFVGRLACTF
                     QGATDTTFYIIAVYFGSVGIRKVRHAVTCGLIADLAGLIAAIFIAYLFFHT"
     gene            821..1252
                     /gene="ybeY"
                     /locus_tag="PLD76_00540"
     CDS             821..1252
                     /gene="ybeY"
                     /locus_tag="PLD76_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOU26526.1"
                     /translation="MAVHFIFDDTKTLRLKKRMIAEWIKNVASGYGKSIGEISYIFCS
                     DNKILAINNEYLQHDYYTDIITFDYTEEQKIAGDIFISVDTVSSNAHIYTNTFSDEMA
                     RILIHGILHLCGIKDKTQQEEQNMRKAEDKALALLKEKILR"
     gene            1249..3123
                     /gene="mnmG"
                     /locus_tag="PLD76_00545"
     CDS             1249..3123
                     /gene="mnmG"
                     /locus_tag="PLD76_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU26527.1"
                     /translation="MKFTYDIIVIGAGHAGCEAACAAANLGSQTLLITMDMNKIAQMS
                     CNPAIGGIAKGQIVREIDALGGFTGIVTDKTSIQFRMLNRSKGPAMWSPRSQCDRAKF
                     IETWRSVIENTANLYVWQDSAVSLVIENGAIAGVKTAMGVTFSCKAVVLTNGTFLNGL
                     LHIGKVQLNGGRVSEPASYGITEQLVAMGFSQGRMKTGTPVRIDARSVDFSQTTIQPG
                     ETDYHKFSYLDTPAHSLKQLPCYTLYTNEKVHQVLEKGLKDSPLYNGQIQSIGPRYCP
                     SIETKIVTFSDKTEHQLFLEPEGESTTEYYLNGFSSSLPIDIQLNALKEIPAFRNVHI
                     FRPGYAIEYDFFDPTQLRHTLETKLVKNLFFAGQINGTTGYEEAAGQGLIAGINAHMN
                     CVGGEKFILHRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADNRLTEKS
                     YTIGLATKERFELFKQKQTWCNEITEFIKNYSVKSSEIDSLLLSVGSTPLKQTTKLMD
                     ILLRPGISLQALIEALPSLQEQVEKITNRKQEIIEQIEISAKYEGYIARERLIADKLQ
                     RLENIKIRDKFNYDDISSLSTEAKQKLKKINPETIAQASRIPGISPSDINILLVLLGR
                     "
     gene            3204..3728
                     /locus_tag="PLD76_00550"
     CDS             3204..3728
                     /locus_tag="PLD76_00550"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOU26528.1"
                     /translation="MTEEQVKKAIRDIPDFPSKGILFKDLTTAFKEPSCMKWFENELY
                     KRYKDKGITKIVGIESRGFVVAPILGNRLNIGFVPMRKKGKLPAETVEISYSKEYGID
                     TIELHKDSLTTDDIILIHDDLLATGGTMIAACKLAKKFNVKKIYVNFLVELDFLDGRK
                     GFDEDIEIDSLLHF"
     gene            3731..5530
                     /gene="uvrC"
                     /locus_tag="PLD76_00555"
     CDS             3731..5530
                     /gene="uvrC"
                     /locus_tag="PLD76_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU26529.1"
                     /translation="MHENLKHIVDTLPELSGVYQYLNQKEEIIYVGKAKNLRKRVSSY
                     FSKSLTSKKTAVLLKNISNIVYTVVDSEEEALLLENNLIKQHQPRYNILLKDDKSFPY
                     ICIKNEPFPRIFKTRNVIKDGSEYYGPFTHMNTLKTLLDICKYQYTIRTCHLHLTKES
                     ILTHKFKVCLEYHIKNCKGPCVNKQTENDYLQQVNEVRSIIKGNIKEVCSSILKEINV
                     LANELRFEEAHILKQKLQILENYQSKWTVVNPSITHVDVFSIQENADESFVNYMNVVN
                     GCIVQAYTVTYKKQLDETKETILGLAIIEMRERFNSKTNEIIVPFVPDGKIKNAEFTV
                     PLRGDKKKLLELSIKNALQYKVDRLKQAEKLNPEQRTTRIVKQLQQDLHLNSLPLHIE
                     CFDNSNTQGTNPVSACVVFKSAKPSKKDYRHYHIKTVEGPDDFKSMQEVVYRRYSRLL
                     EENQPLPQLVIVDGGKGQLSSVYEILKKLDLDTKIAVIGIAKRLEEIFFPNDPIPLYL
                     DKKSETLKVIQHLRDEAHRFGITFHRKIRSKKQTVSSLDSIEGIGEQTKMILLKTFKS
                     VKRIKETPIEELEKIVGKAKAKKIADFYKNSTN"
     gene            complement(5672..7783)
                     /locus_tag="PLD76_00560"
     CDS             complement(5672..7783)
                     /locus_tag="PLD76_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU26530.1"
                     /translation="MRKLFFCFLFLLISIFSFYAESYSAYYLSAEGLSREHLKTALQR
                     RASKHQFLLYDELWNYFQQTDALDNNRVLDRYSSTEYVFSQSSQMDREHAFPKSWWGG
                     ATFYPVYSDLHHLYPSDRSANIAKSNNPLGKVSTNPSFSNGVSKVGPSVISGYSGTVF
                     EPADEYKGDFARAYFYVVTAYEQLFGVWDSPMLDNNTYPVFKTWAIDLLLEWHTNDPV
                     SQLETDRNEQVYLVQQNRNPFIDYPQLVDYIWGNKQQDVFYTSSVITEPTIVSPWQDD
                     TIQFPAIFEDDEIEETVYIRGLNLSEGLSLSITDDANSYFSLAETTVSKTNAEQPNAY
                     PIKIKFSPTEVKEATAILSISGGGATPVSVVLSATSLNRAVAKPEAQPISNVENNAAT
                     LSWTGVYKATNYFVDVYKNGLPKNTTVISEDFEGFYKTSFASNSQGAHSSDVSDSLDY
                     FMSTKEWAGDKIYHANKTAKLGTASAKGYITTPVLDLSHNNGNYTVKFDAKLWVSASE
                     KTTILILHNNVQVQEVTLTSDFQPFEFNFSNGTKESKITLTAKIASNNRFFLDNIRIE
                     QLQPEPIFVKENFDVGDVLFYDLYDLEYDTEYFYRVRANKEGDISANSNTVSFTLPFV
                     NSVLETNYAKYDLFVQDGVIYFTNLENEAKLDFAIFSQIGKTILQDIVLPNQSSSYVL
                     SPGFYILKIGKEHFVKIMVSK"
     gene            complement(7808..>8967)
                     /locus_tag="PLD76_00565"
     CDS             complement(7808..>8967)
                     /locus_tag="PLD76_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU26531.1"
                     /translation="KFFITYITFFCVFFYVHASEFDSDVYYQEAEGKQQYELKTALHK
                     IIRGHTRVSYTPGIWQAYEKTDLRQNGTVWCIYTSCPFNYGVHPDYVEGYQCGSTGMG
                     YTECFCYSREHSFPKSFWGGGTALSDTVYTDVFHIYPASGRVNSVLRNDNVYANVGTI
                     IPKNNDDNPTSNGSKLGYSSVVGYDGQAFEIADEYKGDIARTYFYIATRYQHLIPQWY
                     TTSTGTKPNNARFILASNTSSVFQSWYINMLLAWHRQDPVSAKEIDRNNAVYTHVQNN
                     RNPFIDHPELVEYIWGNKQAEKWRQPTSIKTTTENLFSIYPASDTNFFFCDIKSDADI
                     RFVIQDVSGKIIRRGVFSPPYQFFIKDLHKGIYIMHVKENRMLSKTTFFVK"
BASE COUNT         3158 a         1625 c         1564 g         2620 t
ORIGIN      
        1 tgtttttggt tttgaacaca tctggattaa cgattattcc catttctatt atggtatacc
       61 gagcacaaat gggagcagca gatccatcgg atatttttat acccatactt ctttctactt
      121 ttgtgagcac attggcgggt attatagccg tatccattgc tcaaaaaata aacctattta
      181 ccaaaacaat tttactttat ttaggaagta tcacactttt gctaactgga gtgatttggg
      241 gttttactgt attgagttct gaaaaactaa acatggtatc ttctgtagcc gcaaacatta
      301 ttttatttag cataattatt ggatttattt tattggcaat acgcaaaaaa ataaatgtgt
      361 acgattcctt tattgaagga gctaaagatg ggttttcaat tgccgttaaa attattccgt
      421 ttttagtagc tatcttggta ggtataggcg tgtttagggc ttcaggagct atggacagtt
      481 taatgtcggg cattgcttgg ctatttacat caatcggatt aaatgcaaat tttgtagaag
      541 ctttacctgt tgcctttatg aaacccttga gtggagcagg agctcgtggg ctaatgatag
      601 atgcaatgaa ccagcatggt gtagattcat tcgttggtcg tttggcatgt acgtttcaag
      661 gggcaacgga tactacgttt tatataattg cagtgtattt tgggtcagta ggtatacgaa
      721 aagttcgtca tgctgtaaca tgcggattaa ttgctgattt agctggatta attgctgcta
      781 tttttattgc atatttattc tttcatacct aattatcact atggcagtac attttatatt
      841 tgatgatact aaaacgcttc gtttaaaaaa aagaatgata gctgaatgga taaagaatgt
      901 agcctctgga tatggaaaat ctattggtga aatcagctat attttttgtt cagacaacaa
      961 aattcttgct atcaataacg agtacctaca acatgattac tatacagata tcataacctt
     1021 tgattacact gaagaacaaa aaatagcagg agatatattt atcagtgtag atacagtaag
     1081 tagcaatgca cacatttaca ccaacacatt tagcgatgaa atggcacgca ttttaataca
     1141 tggcattttg catttgtgtg gcataaaaga taagacacaa caagaagaac aaaacatgcg
     1201 aaaggctgaa gataaagcct tagccttatt gaaagaaaag atactacgat gaaatttacg
     1261 tatgatataa tagtgattgg tgctggtcat gcaggatgtg aagctgcatg tgcggctgca
     1321 aaccttggtt cgcagacctt gctcataaca atggatatga acaaaattgc acaaatgagt
     1381 tgcaatcccg ccatcggagg aatagctaaa gggcagatag tgagggaaat agatgcactt
     1441 ggaggcttta caggcattgt tacagacaaa acatccatcc aatttaggat gcttaatcgc
     1501 tccaaaggac ctgctatgtg gagccctcgt tcgcaatgcg acagagctaa atttatagaa
     1561 acatggcgat cggttatcga gaatactgcc aatttgtatg tatggcaaga ttctgcggtg
     1621 tcgttagtaa tagaaaatgg tgcaatagct ggagtaaaaa cagctatggg agttactttt
     1681 tcgtgcaaag ctgttgttct aacgaatggt acatttttaa atgggctttt acatattggc
     1741 aaagtacaac taaacggagg aagagtttca gaaccagcaa gttatggcat tacagaacaa
     1801 ttggtagcaa tgggtttttc acaaggaagg atgaaaacgg gtacaccagt acggatagac
     1861 gcacgaagcg ttgattttag ccaaacaact attcagcctg gcgaaacaga ttaccacaaa
     1921 ttttcctatc tagatacgcc tgcacatagc ttaaaacaat tgccttgcta cactctttat
     1981 accaacgaaa aagtacacca agtattagag aaaggactaa aagattcccc tctatataat
     2041 ggacaaatac aaagtatagg tccgcgttat tgccctagta ttgaaactaa aattgtaacc
     2101 ttttctgaca aaacagaaca ccaactcttt ttagaacctg aaggtgaaag tactacggaa
     2161 tattatttga atggattttc atcatccttg cctatcgaca ttcaattaaa tgctctcaaa
     2221 gaaataccag cttttcgcaa tgtacatatt ttcagacctg gatatgcaat agaatacgat
     2281 tttttcgacc ctacccaatt aagacatacc ttagaaacaa aactggtaaa aaacctgttt
     2341 tttgcaggtc aaataaatgg cacaacaggg tacgaagaag ctgctggaca ggggttaatt
     2401 gcagggatta atgcacatat gaattgtgtt ggtggagaaa agtttatctt acatagagac
     2461 gaagcatata ttggtgtttt aatagatgat ttggtaacca aaggagttga cgaaccttat
     2521 cgcatgttta catcgcgtgc tgaataccgt atcttgctac gacaagacga tgcagacaat
     2581 cgtttaactg aaaaatcata taccataggt ttagccacaa aagaacgatt tgaattgttt
     2641 aaacaaaaac aaacctggtg taacgaaata acagaattta ttaaaaacta ctctgttaaa
     2701 agcagcgaaa ttgattcact gctactctca gttggctcca ccccattaaa acaaacaaca
     2761 aaactaatgg atattctttt gcgaccaggt attagcctac aagcattaat tgaagcatta
     2821 ccaagcctac aagaacaagt agaaaaaata acaaatagaa aacaagaaat tattgagcaa
     2881 atagaaatat ctgccaaata cgaaggatat attgcaagag agcgtttaat tgctgataag
     2941 ctacaacgat tagaaaacat aaagattaga gacaaattca actacgacga catcagctct
     3001 ttatcgacag aagcaaaaca aaaactaaaa aaaatcaacc cagaaacgat agcccaagct
     3061 agcagaatac caggaatatc tcctagcgat atcaatattt tattagtgtt attaggacga
     3121 taaatttgtt tcacgtgaaa caaatttacc ttaaaagaac acaatgttcc acgtgaaaca
     3181 tacaacaaaa aaaacaaaaa catatgaccg aagaacaagt aaaaaaagca attagagaca
     3241 ttccagattt tccaagtaag ggaatattat ttaaagacct aactacagca tttaaagaac
     3301 catcctgcat gaaatggttt gaaaatgagc tatacaaaag atacaaagat aaaggaatca
     3361 ccaaaatcgt tggtatagaa tctcgtggct tcgtagtggc tccgatatta ggaaataggc
     3421 tcaatatcgg ctttgttcct atgcgtaaaa aaggcaaact accagcagaa acagtagaaa
     3481 taagctactc taaagaatac ggcatcgaca ccatagaact ccataaagat tctcttacta
     3541 cagacgatat tatcttgatt cacgatgact tgctagctac tggtggcaca atgattgccg
     3601 catgcaaatt agctaagaaa tttaatgtaa aaaaaatata cgtcaatttt ttagttgaat
     3661 tggattttct tgacggtcgt aagggatttg acgaagacat agaaatagat tctctgctcc
     3721 atttctaact atgcacgaaa atctcaaaca tattgttgat acacttccgg aactttcggg
     3781 agtgtatcaa tacttgaatc aaaaagaaga aataatatac gtagggaagg caaaaaactt
     3841 gcgaaaacgt gtctcttcgt atttttccaa atcgcttacc tcaaaaaaaa cagccgttct
     3901 tctaaaaaat atatccaata tagtttacac ggttgttgac agtgaagaag aagccttatt
     3961 gctcgaaaac aatttaatca aacaacacca accacgctac aacatcttgc taaaagatga
     4021 taagtctttc ccttacattt gtatcaaaaa cgaaccattt ccacgtattt tcaaaacacg
     4081 caatgtgata aaagacggct ctgaatacta tggaccgttt acccacatga ataccttgaa
     4141 aacgctgtta gatatttgca aataccaata cactattcgt acatgccatt tacacctaac
     4201 caaagaatca atactcactc ataagtttaa agtctgttta gagtaccata taaaaaattg
     4261 caaaggtccc tgtgttaaca aacagacaga aaatgactac ctgcaacaag ttaatgaggt
     4321 acgctcaata ataaaaggca acataaaaga agtatgctcc tccatactga aagaaattaa
     4381 cgtactagca aatgaattac ggtttgaaga agcacacata ctgaaacaaa agttacaaat
     4441 tcttgaaaac taccaatcaa aatggacagt ggtaaatcca agcattacac acgttgatgt
     4501 atttagcata caagaaaatg ccgatgaaag ctttgtaaat tatatgaacg ttgtaaacgg
     4561 ttgtattgta caagcataca cagtaacata caaaaagcaa cttgatgaaa caaaagaaac
     4621 aattttagga ctggcaatta tcgaaatgcg agaacgattt aatagcaaaa caaacgaaat
     4681 tattgtccca tttgtgccag atggaaaaat aaaaaatgct gaattcaccg tacctttacg
     4741 tggcgataaa aagaaactgc tcgaattgtc gataaaaaat gcacttcaat ataaagttga
     4801 cagactaaaa caagcagaaa agctaaaccc agaacaacga acaacaagaa tagtaaaaca
     4861 actacaacaa gacctacatc ttaactctct cccattgcat atcgaatgct ttgacaattc
     4921 aaatacacaa ggaaccaatc ccgtatcagc atgtgttgta tttaaatcag ccaaaccatc
     4981 gaaaaaagat taccgacact accatataaa aacagtggaa ggacctgacg actttaagag
     5041 catgcaagaa gtggtttaca ggcgatattc gcgattattg gaagaaaacc agccattacc
     5101 ccaattagta atagtggatg gcggaaaggg gcagctaagc agcgtatatg agatattgaa
     5161 aaaacttgac ttggatacaa aaattgctgt aattggaatt gccaaaagac ttgaagaaat
     5221 atttttcccc aacgacccca ttccgctcta tttggacaag aaatccgaaa cattaaaagt
     5281 catacaacat ttgcgtgacg aagcccatcg ttttggaatc acctttcata gaaaaatacg
     5341 cagtaagaaa caaacagttt cgagcttaga cagcatagag ggaattggcg agcaaacaaa
     5401 aatgatattg ctaaaaacat tcaaaagtgt aaaacgaata aaagaaacgc caattgaaga
     5461 gctagaaaaa attgtaggta aagcgaaagc taaaaaaata gccgactttt ataaaaacag
     5521 tacaaattag gctattttat cctttttcac gattgcaaaa aatggctata ttatcctgat
     5581 tacaagacag ataagcttat tttaacttct ctagaattaa ctatttagtt taaatcgcta
     5641 ccttgtacag aaaaacggca ttcatttgct gttattttga caccataatt ttaacaaaat
     5701 gttctttccc tattttcaga atataaaatc ctggcgataa cacataggat gatgattgat
     5761 taggcaaaac gatgtcttgt aagatggttt tgccaatttg agaaaaaata gcaaaatcaa
     5821 gcttcgcttc gttttccaag tttgtaaaat aaattacccc atcttgtaca aacaaatcat
     5881 actttgcata atttgtttcc aaaacagaat ttacaaaagg tagcgtaaag gacaccgtat
     5941 tcgaattggc agaaatatct ccttccttgt ttgctcttac gcgatagaaa tattcagtat
     6001 cgtattccaa atcatacaaa tcgtaaaaca atacatctcc tacatcgaaa ttctctttta
     6061 caaaaatagg ttcgggttgc aattgttcaa tacgaatatt gtctaaaaag aaacggttgt
     6121 ttgatgcaat ttttgctgtt aatgtaatct tcgattcttt tgtgccgttg ctaaaattaa
     6181 attcaaatgg ctgaaaatca ctggttaatg taacttcttg cacttgtaca ttgttgtgca
     6241 aaataaggat agtagttttt tcggaagcac ttacccataa ttttgcatcg aatttaaccg
     6301 tgtaattgcc gttattgtgg gataaatcca gcacgggtgt agttatatat ccttttgcag
     6361 aagcagtacc taatttggcg gttttattcg catgatagat tttatcgcca gcccactcct
     6421 ttgtactcat aaaataatcg aggctatcag acacatcgga actatgagca ccttgcgaat
     6481 tgcttgcaaa acttgtttta taaaaaccct caaaatcctc agaaatcaca gtggtatttt
     6541 ttggcaagcc atttttatac acatcaacaa aataattggt agccttgtac acccccgtcc
     6601 atgataaggt agccgcattg ttttcaacat ttgatatggg ctgtgcttct ggtttggcta
     6661 ctgccctatt taaggatgta gctgataaaa cgacagagac aggtgtggct cctcctccag
     6721 aaatactaag aatagcggta gcctctttaa cctcggtagg cgaaaattta atcttaatag
     6781 gatatgcatt tggttgttct gcatttgttt tagaaacagt agtttcagcc aaagaaaaat
     6841 aactattcgc atcatctgtg atagaaagag ataagccttc tgataaattc aaacctctta
     6901 tatagaccgt ttcctctatc tcatcatctt caaaaatagc aggaaattgt atcgtatcgt
     6961 cctgccacgg agaaactatg gtgggttctg ttattacact agaagtgtag aatacatctt
     7021 gttgtttatt tccccaaata taatctacta gttgagggta atcgataaat ggatttctat
     7081 tctgttgaac aagatatact tgttcgttcc tatctgtttc aagctgcgaa actgggtcat
     7141 tagtatgcca ctctaacaac aaatcgatag cccaagtctt aaaaacagga taggtattat
     7201 tgtctaacat gggcgaatcc catacgccaa acaactgctc ataggctgtt acaacataaa
     7261 aataagcccg agcaaaatca cctttgtatt cgtctgcagg ctcaaaaact gttcctgaat
     7321 atcccgatat tacagagggt ccaactttac ttaccccatt agaaaatgaa ggattagtag
     7381 agactttccc taatggatta ttgcttttgg ctatattagc actcctatcg ctaggataga
     7441 ggtgatgcaa atctgaatac actggataaa aagtggctcc accccaccag ctttttggaa
     7501 aagcatgttc cctatccatt tgtgatgatt gggaaaaaac atattcggta gatgaataac
     7561 ggtctaacac acgattgtta tccaatgcat ctgtttgctg aaaataattc caaagctcat
     7621 catataacaa aaattgatgc ttactcgccc ttcgttgcaa tgcagttttt aaatgttctc
     7681 tacttaatcc ttcagctgat aaataataag cactatatga ttcagcataa aaagaaaaga
     7741 tactaatcaa aagaaataag aagcaaaaaa ataattttct cataatgctg tgtactttaa
     7801 aaaaatatta tttcacaaag aaggtagttt tacttaacat tctgttttct tttacgtgca
     7861 taatataaat acctttatgc aaatctttaa taaaaaactg atatggagga gaaaacacgc
     7921 ctctacgaat tatttttcct gacacatctt gaataacaaa cctaatatct gcatccgact
     7981 taatatcaca gaaaaaaaaa ttagtgtcgc ttgctggata aatactgaat aaattttctg
     8041 ttgttgtttt tattgatgtt ggctgacgcc atttttcagc ttgtttattt ccccaaatat
     8101 attcgaccaa ttcagggtga tcaataaaag gattacggtt attttgtacg tgtgtataaa
     8161 cggcattatt tctatctatt tcttttgcag atacggggtc ttgtctatgc cacgcaagta
     8221 gcatattgat ataccatgac tgaaaaacag aactcgtatt tgatgctaat ataaagcgtg
     8281 cattattcgg ttttgttccc gtcgaagtgg tataccattg cggtataaga tgttgataac
     8341 gagtagctat ataaaaataa gtacgtgcga tatcaccctt gtattcatct gctatttcaa
     8401 aagcctgacc atcgtatcct acaacagaag aatagcctaa tttagaaccg tttgaagtag
     8461 gattgtcgtc attatttttt ggaattattg ttccaacatt cgcatataca ttatcatttc
     8521 gaaggacgct atttaccctc ccactcgcag gataaatatg aaacacatcg gtatatacag
     8581 tatcagataa tgctgttcct ccgccccaga aacttttagg aaaagaatgt tctctgctat
     8641 aacaaaaaca ctccgtatac cccattcccg tagaaccaca ttgatagccc tctacataat
     8701 caggatggac gccataatta aatgggcacg aagtatatat gcaccaaacg gttccatttt
     8761 gcctcaaatc tgttttttca taggcttgcc aaatacctgg tgtatagctt accctagtat
     8821 gcccgcgtat tattttgtgt aatgctgttt ttagttcata ttgttgtttt ccctctgctt
     8881 cttggtaata cacatcacta tcaaactctg atgcatgtac ataaaagaaa acacaaaaaa
     8941 aagttatgta tgtaataaaa aacttca
//
LOCUS       DAOSMY010000011         8878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_7844_length_8878_cov_4.061090, whole genome shotgun sequence.
ACCESSION   DAOSMY010000011 DAOSMY010000000
VERSION     DAOSMY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_8878_cov_4.061090"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PLD76_00570"
     CDS             complement(<1..951)
                     /locus_tag="PLD76_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOU26532.1"
                     /translation="MFENLSERLERSFKILKGQGKITEINVAETLKDVRKALLDADVI
                     YKIAKTFTDTVKEKALGMNVLTAIKPNELMVKIVRDELALLMGGTSVDINTKGSPAVI
                     LMSGLQGSGKTTFSGKLAKMLKSKKGKNPLLAACDVYRPAAIEQLKVLGEQIGVPVYS
                     EIENKNPIQIAQNAIAFAKKNGHDLLIIDTAGRLAIDEQMMNEIAAIKQAIQPQEILF
                     VVDSMTGQDAVNTAKEFNDRLDFTGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMEAIDQFHPERMADRILGMGDIVSLVEKAQEQYDEEEARR"
     gene            1175..2962
                     /gene="lepA"
                     /locus_tag="PLD76_00575"
     CDS             1175..2962
                     /gene="lepA"
                     /locus_tag="PLD76_00575"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU26533.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEETSTVTGKEMQAQVLDNM
                     DLERERGITIKSHAIQMNYIYEGEKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYMAIEHDLEIIPIMNKMDMDSAMPEEVEDQIVELLGCKSDEIIKA
                     SGKTGLGIQEILQAIVKRIHPPKGDPEAPLQCLVFDSVFNSFRGIITYMKIENGTIRK
                     GDLVKFFNTGKEYFADEIGVLRLGMEPREELRCGDVGYIISGIKVSKEVKVGDTITHV
                     KRPCEKGIEGFEEVKPMVFAGIYPIDSEDFEDLRASMEKLQLNDASLTFEAESSAALG
                     FGFRCGFLGMLHMEIVQERLDREFDMDVITTVPNVQYKIYTRKNEVLDVHNPSGMPDI
                     TLIDRIEEPYIRASIITRTDFIGSIMSLCLGKRGELIKQDYISSNRIELIYDMPMGEI
                     VFDFYDKLKSISKGYASFDYHISGFRPSKLVKLDILLNGESVDALSSLTHFDNAVTIG
                     RRMCEKLRELIPRQQFDIAIQAAIGSKIIARETIKAVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFMEVLKLD"
     gene            3011..4339
                     /locus_tag="PLD76_00580"
     CDS             3011..4339
                     /locus_tag="PLD76_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOU26534.1"
                     /translation="MEHVSDVAQHVAMPIWASAPFALMLIMIAIGPLLWEHWWEQNKN
                     KLLVSFLLSVPTVIFLIINGFSHDLEHQIMFDYVPFIILLTSLYVISGGLFLTGDIQA
                     KPKINTLFMAIGYVLASIMGTTGAAMLLIRPLITTNSQRMYKTHTILFFIAAVANAGG
                     LLTPLGDPPLFLLYLRGADFGWFLHMLPEWLFIGTVLIGLYYLLDRRYYKKEKPEAIE
                     SDNANVEPLRIGGSKNFFLLIGVILCVAFVNVNYIPEMGNPHGSLWIKYLREIILIGF
                     ILVSMDITKKKTRELKGFSWTPIYEVAALFLGIFITMVPALLYLKENAASFGLSESWQ
                     FYFMTGSLSSFLDNAPTAVAMHGVALGLVQEGAAVAEVAGVPELLLTAISMGSVLFGS
                     MTYIGNGPNFMVKAIAESSGIKMPSFFGYIFKFSLVILLPLYILTYFLFV"
     gene            4487..5833
                     /gene="purB"
                     /locus_tag="PLD76_00585"
     CDS             4487..5833
                     /gene="purB"
                     /locus_tag="PLD76_00585"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU26535.1"
                     /translation="MTNLNLLTAISPVDGRYRSKVDELTNYFSEYALIRYRVQVEIEY
                     FIALCELPLPQLNNIEKKVFPVLRSIYTSFSVADAQKVKDTESITNHDVKAVEYFIKD
                     KFDEIGLSDYKEFIHFGLTSQDINNTAVPLSIKDCLYTVYLPVLQQLVDELEQLATKW
                     EHIPMLAKTHGQPASPTRLGKEIRVFVSRLEQQIVLLKQTPISAKMGGATGNFNAHHV
                     AYPTINWIDFGNSFVKNYLGLEREPYTTQISNYDNLAALFDAIKRINTILIDMNRDFW
                     TYISMEYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGIANAILEHLAGKLPISRLQ
                     RDLTDSTVLRNVGVPMAHNLIAIKSTLKGLSKLILNEASLKRDLDNCWNVVAEGIQTI
                     LRRENYPKPYETLKELTRTNTEVNAESIASFIETLQVSESVKAELRAITPHNYTGL"
     gene            5851..7686
                     /locus_tag="PLD76_00590"
     CDS             5851..7686
                     /locus_tag="PLD76_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU26536.1"
                     /translation="MKRFIILLKRFIPPYKKYVGLNVLFNLLSTIFSLFSFALIIPIL
                     EILFNTKAVVYELQSTHNVPMSELFDVLKNNLYYYTTELIDKSGALTVLIILSSFLIL
                     MTFLKVATAYLGSFFMVSIRTGVLRDLRNMIFAKIVHLPIGFFNEERKGDIMSRMTLD
                     VNEVEASIMSSLDMLFKNPLMILTYIIVMFTISWELTLFVMVLLPISGYFIGQVGKSL
                     KRKSLEGSILTGELTSQIEETLGGLRIIKAFNAEKKIISRFVALNNRLRGTLNKIQGR
                     YLLAHPMSEFMGTVVIATVLCYGGNLILTSNSEALTASVFIYYILIFYSIINPVKELS
                     KAAYSVQKGMASLERIDKILQTQDSLHTVNGKEISGFNHSIEFKNVSFKYQNAWVLRN
                     INLTIEKGKTIALVGQSGSGKSTLVDLIPRFWDVVEGEICIDGVNIKEINLHSLRDLM
                     GNVNQEAILFNDTFYNNIAFGVENATMDEVIAAAKIANAHDFISATEQGYDSNIGDRG
                     SKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKLVQEAIEKLMKNRTSVVIA
                     HRLSTIKNADEICVLLDGKIVERGTHEALLQENGVYKKLQETQTV"
     gene            complement(7750..8475)
                     /locus_tag="PLD76_00595"
     CDS             complement(7750..8475)
                     /locus_tag="PLD76_00595"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOU26537.1"
                     /translation="MFYQDSIHKKSVNQKQLALLVDPDKYSQESLALFVELAKETLPD
                     CIMVGGSLVAGSVEQVVQYIKLHTNIPVVLFPGNSHQLCNEADALLLLSLLSGRNADF
                     LIGQHVVAAKAIRDSGIESISTGYILIDGGCSTSVEYMSNTKPIPCDKVEIIVATAIA
                     GELIGNRMVYLEAGSGAINPVSKEAIQAVRKAISLPIIVGGGIRTVEQVKDAYLAGAD
                     MIVVGNALEKDRFFMRDLAQIRG"
     gene            complement(8477..>8878)
                     /locus_tag="PLD76_00600"
     CDS             complement(8477..>8878)
                     /locus_tag="PLD76_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOU26538.1"
                     /translation="LVDNPNYLYHSLAMFDRGMIDILTKTDKFQDSGVYKCWDHSGDQ
                     VLAFARGGELLFVFNFHPYNSFTDYGMLVDKGEYEVVLNTDAKEYGGFGLCDDSIRHF
                     TIPTNVKGEEKEWIKLYLPSRTAMVLRKVSK"
BASE COUNT         2920 a         1590 c         1562 g         2806 t
ORIGIN      
        1 cctacgagcc tcttcttcgt catattgttc ttgggctttt tctactaacg acacaatatc
       61 acccataccc aaaatcctat ctgccatacg ttcgggatga aactgatcaa tggcttccat
      121 tttttcgccc gttcctacaa atttgatagg tttatccact accgaacgaa tagataaagc
      181 cgctccacct cgggtatcac catctaattt tgtaagtaca acacccgtaa aatcaagtct
      241 atcgttaaat tcttttgcag tattcacagc atcttgaccc gtcatcgagt ccaccacaaa
      301 caaaatctct tgcggttgaa tagcttgttt aatggcagct atttcgttca tcatttgctc
      361 atcaatagcc aaacgacccg ctgtatcaat aattaacaaa tcgtgtccgt tctttttagc
      421 aaaagcaatg gcattttgag ctatctgaat gggatttttg ttttctattt cactataaac
      481 aggaacgcct atttgttcgc caagcacttt taattgctcg atagcagctg gacgatatac
      541 atcacaagca gcaagtaaag gattttttcc tttctttgat ttcagcattt tggctaattt
      601 gcccgagaaa gtagtttttc ctgaaccttg caaaccagac attaaaatta cagcaggcga
      661 acctttggta ttaatatcca cggatgttcc acccatcaac aaggctaact catcacgcac
      721 aattttaacc atcaactcgt tgggcttaat tgctgttagc acattcattc ccaaagcttt
      781 ttctttaacc gtatcggtaa atgtcttggc tattttatag ataacatcgg catccaaaag
      841 agctttacgc acatctttta atgtttctgc tacatttatt tcagtaattt taccctgccc
      901 tttaagaatt ttaaacgatc gttcgaggcg ttcacttaaa ttttcaaaca taattatctt
      961 cggtttagtt taataaatca tcaaaaagac agcaaatagt cctttttttg gaaatagctc
     1021 acaaaattag tcaaaatttg tgattttacg cacataaatg tagaataatc atcgaaaatt
     1081 gatttataaa aaaacagttt tttcatctct tttttatgtt ttagtctata attattcact
     1141 atctttgcgg gtcttaaaat aataattaga acggatgaaa aatattcgca atttttgtat
     1201 tattgcccat attgaccatg gtaaaagtac cttagccgat cgtctgctcg aagagactag
     1261 cacagttaca ggcaaagaga tgcaagctca agtacttgat aatatggatt tggaacgaga
     1321 aagaggcatt accatcaaaa gtcatgctat tcagatgaac tacatatatg aaggagaaaa
     1381 atatgtactc aatcttattg acactccagg acatgtagat ttttcgtatg aagtatctcg
     1441 ttcaattgct gcttgcgaag gtgctttgtt gattgtagat gctacacaag gcattcaggc
     1501 tcaaaccatc tctaacctat acatggctat tgaacatgat ttggaaatta ttcctattat
     1561 gaataaaatg gatatggata gtgccatgcc tgaggaagtt gaagaccaaa ttgtagaatt
     1621 gctgggatgc aaatcggatg aaattataaa agctagcgga aaaaccggtt taggtataca
     1681 agaaatatta caagcaattg tcaaacgcat acatccgccg aaaggagacc cagaagctcc
     1741 tttgcaatgt ttggtatttg attctgtatt caactctttc agaggaatta ttacctatat
     1801 gaaaattgaa aacggaacta tacgcaaagg cgatttagta aaattcttta atactggaaa
     1861 agagtatttt gctgatgaaa ttggcgtgct tcgtttaggt atggaaccaa gagaagaatt
     1921 acgttgtggt gatgttggat atattatttc aggcattaag gtatctaaag aagtaaaagt
     1981 tggcgacact attacacatg taaaacgtcc ttgcgaaaaa ggcattgagg ggtttgaaga
     2041 agtaaagcca atggttttcg ccggtatata tcctattgac tcagaggact ttgaagacct
     2101 aagagcgtca atggagaaat tacaattgaa tgatgcgtct cttacttttg aagcagagtc
     2161 atcagccgct ttaggttttg gctttcgttg cggatttcta ggcatgttgc acatggagat
     2221 tgtacaagaa cgacttgatc gtgaatttga catggatgta attacgacag tacccaacgt
     2281 acagtataaa atatatacgc gtaaaaacga agtgctcgac gttcacaatc catcaggaat
     2341 gccagatata acattgattg acagaataga agagccttat atacgagcgt caattattac
     2401 acgaacggat tttattggtt ctatcatgag tttgtgtttg ggaaaaagag gagaattgat
     2461 aaaacaagat tatatttcat ctaatcgtat cgaattaatt tacgatatgc ctatgggaga
     2521 aatagtcttt gatttttatg ataaattgaa gtccatttcc aaaggatatg cttcgtttga
     2581 ttaccatatc agcggatttc gtccttcaaa attggtaaaa ctagatattt tattaaatgg
     2641 agaatcagta gatgccctgt catcactcac tcactttgat aatgcggtta caattggtcg
     2701 tagaatgtgc gagaaattac gcgaattaat tcctcgtcag cagtttgata tcgctattca
     2761 agctgccatt ggttcaaaaa ttattgcccg cgaaaccatt aaagctgtac gcaaagatgt
     2821 tacagcaaaa tgttatgggg gcgatatttc acgaaagcgt aaactactgg agaaacagaa
     2881 aaaaggaaaa aaacgaatga agcaaattgg ttcagtagaa gtaccacaaa aagcgtttat
     2941 ggaggtactt aaattagatt agttttcaat attttttaaa taaattattt taccttaaaa
     3001 ttcaatttct atggaacatg tttctgatgt tgcacagcac gttgctatgc caatttgggc
     3061 atctgcccct tttgcattga tgctcattat gattgccatt ggtcctcttt tatgggaaca
     3121 ctggtgggag caaaacaaaa acaaattatt ggtttcattc ttactgagtg taccaacagt
     3181 tatctttctg attataaatg gatttagcca tgatttagaa catcaaatca tgttcgatta
     3241 tgttcctttt ataattttac ttacatcttt gtacgttatt tctggtggct tatttcttac
     3301 gggagacatt caagccaaac caaaaattaa caccttattt atggcaattg ggtacgtgtt
     3361 agcgtctatt atgggtacaa caggagcagc catgctctta attcgcccat taattacaac
     3421 caatagccaa cgtatgtata aaacacatac cattctattt tttattgctg ccgtagcaaa
     3481 tgcaggagga ttacttactc cactcggaga tcctccatta ttcctattat atctacgtgg
     3541 agctgatttt ggttggtttt tacatatgct tcctgaatgg ttatttatag gtaccgttct
     3601 aattgggtta tactatttgt tagataggag atattataaa aaagaaaaac cagaagcaat
     3661 agaaagcgat aatgcaaacg tagaaccttt acgtattggg ggtagtaaga atttcttttt
     3721 gctgattgga gtaattttat gtgtagcttt tgtcaatgta aattatattc cagaaatggg
     3781 caatcctcat ggatctcttt ggattaaata tttacgcgaa attattttaa ttgggtttat
     3841 tttagtatcc atggatatta ccaaaaagaa aacacgcgaa ctgaaaggat tctcatggac
     3901 tcctatttac gaagttgcag ctttattttt aggtatcttt attacgatgg tacctgcatt
     3961 attgtattta aaagaaaatg cggctagttt tggattaagt gaaagctggc aattctactt
     4021 tatgacaggt agtttaagtt cgtttttaga taatgctcct acagctgtag cgatgcatgg
     4081 agtagcctta ggactagtgc aagaaggagc agctgtagca gaagtagcag gcgttccaga
     4141 actgttatta acagcaatat ctatggggtc tgttttattt ggttctatga cctacattgg
     4201 taatggtcct aactttatgg taaaagctat agccgaaagt agtggcataa aaatgcctag
     4261 tttctttggc tatattttta aattttcttt ggttatttta ctcccactct acattcttac
     4321 ttatttcttg tttgtataaa aaacaaaata tatacgtaat aaatgacttg gacaatggtt
     4381 ccaagtcatt tattttttac accaatatca gatatcttac catcggatat attgtaatta
     4441 tgtgtaaata attgtacctt tgcacttcta ttaattatct atttatatga ctaacttaaa
     4501 tttactaact gctatctcac ccgttgatgg tagataccgt tccaaagttg acgaacttac
     4561 aaactatttt tctgaatacg cactaattag atacagagta caagtagaaa tcgaatactt
     4621 tatagctttg tgcgaacttc ctttacctca acttaacaac atagaaaaaa aggtttttcc
     4681 agttctacgc tctatataca cctctttttc agtagctgat gctcaaaaag taaaagacac
     4741 agaaagcatc accaaccatg atgtaaaagc agtagaatat tttattaaag ataaatttga
     4801 tgaaattggg ctatctgatt ataaagaatt tattcatttc ggactcacat cccaagatat
     4861 taataataca gctgttccat tatcaattaa agactgccta tacactgtgt atttgcccgt
     4921 tttacagcaa ttggtagatg aattggagca attagcaacg aaatgggaac acatccctat
     4981 gctggctaaa actcacggac aaccagcatc tcctacacgt ttaggcaaag aaattagggt
     5041 atttgtaagc cgactagagc aacaaattgt gttgttaaaa cagactccta tttcagcaaa
     5101 aatgggtgga gcaacaggaa attttaacgc tcaccatgta gcctatccta ctatcaattg
     5161 gattgacttt ggtaattctt ttgtgaaaaa ttatttaggc ttagagcgag agccatatac
     5221 aacccaaata tctaattacg ataatttagc agcacttttc gatgccataa aacgtatcaa
     5281 tactattctg atagacatga atagggattt ctggacatac atttcgatgg agtatttcaa
     5341 acaaaaaatt aaagcagggg aagttggttc atctgcgatg ccgcacaaag taaatccaat
     5401 cgattttgaa aatgcagaag gaaacttagg tattgccaat gctattttag aacatttagc
     5461 gggcaaatta cccatatcac gcttgcaaag agaccttacc gactcgactg ttttacgcaa
     5521 tgtaggggtt ccaatggctc ataatttaat tgctatcaaa agtaccctca aaggacttag
     5581 taaattaatt ttgaatgaag caagcttaaa aagagactta gataattgtt ggaatgtagt
     5641 agccgaaggg attcaaacca tcttacgtag agaaaattat ccaaaaccat acgaaacact
     5701 aaaagaatta actcgcacca ataccgaggt taatgccgaa tctattgctt catttatcga
     5761 gactttgcaa gtaagcgaat ctgtaaaagc agaattgcga gctattacac cgcataatta
     5821 tactggatta taataaacca tactgtataa atgaaacgat ttattatttt actgaaacga
     5881 tttatccctc catacaaaaa atatgtaggg ctaaatgttt tgttcaattt actatctact
     5941 atttttagtt tgttctcctt tgcacttatt attccgatac tcgaaatatt gtttaataca
     6001 aaagccgtag tatatgaatt acaaagcaca cacaatgtgc caatgagcga attgtttgat
     6061 gtactgaaaa ataatttata ttattacaca accgaactga ttgacaaaag tggagcgtta
     6121 accgtactta ttatcctctc ttcttttctc atattaatga catttttgaa agttgcaaca
     6181 gcctacctag gtagtttctt tatggtatcc atacgcacag gggtattacg cgacttgcga
     6241 aatatgattt ttgcaaaaat tgtgcattta cctattggtt tttttaacga agaacgaaaa
     6301 ggtgatatca tgtcacgtat gaccttagat gtaaatgaag tagaagcttc aatcatgagt
     6361 tcgctggata tgttgtttaa aaaccccttg atgattctta cttacattat cgttatgttt
     6421 acaataagtt gggaacttac gttatttgta atggtactat tgccaataag tggatatttt
     6481 attggacagg taggaaaatc gcttaaacga aaatcgctcg aagggtcaat tcttacagga
     6541 gaattaacct cccaaataga ggaaacattg ggtggattac gcattattaa agcattcaat
     6601 gcggaaaaaa aaatcatttc acgatttgta gcactaaaca atcgcttacg aggcacactc
     6661 aataaaattc agggtcggta tttattagca catccaatga gcgaatttat gggtactgtg
     6721 gtaatagcaa ccgtgttgtg ttacggtggc aatcttattt taacgagcaa ttccgaagca
     6781 ttaactgcat ctgtatttat ttattacatc ttaattttct acagcattat caatcctgta
     6841 aaagaactat caaaagctgc ttatagcgtg caaaaaggca tggcatcgct cgaaagaatt
     6901 gataagattt tgcaaacaca agattcatta cacaccgtaa acggaaaaga gatttctgga
     6961 tttaatcatt cgatcgaatt taaaaatgtg agttttaaat atcaaaacgc atgggtatta
     7021 cgtaatatca acctaacaat tgaaaagggt aaaaccattg cattagttgg tcaatcaggc
     7081 tcaggaaaat ctacacttgt cgatcttatt ccacgttttt gggatgtggt agagggagaa
     7141 atatgtattg atggtgtaaa tattaaagaa attaacttgc atagcttacg cgatttgatg
     7201 gggaatgtga atcaagaagc cattttattt aatgatactt tctataataa tattgcattt
     7261 ggtgtagaaa atgccacgat ggatgaggta attgccgcag caaaaattgc caatgcacac
     7321 gattttattt cagctaccga acaagggtat gattcaaaca ttggagatag aggcagtaag
     7381 ttatctggtg gacaacgtca acgtataagc attgctagag caatattaaa aaaccctcca
     7441 atattaatat tagacgaagc cacatctgct ttagacactg aatcagaaaa attagtgcaa
     7501 gaagccatcg aaaaactgat gaaaaataga accagtgttg ttatagctca ccgactatct
     7561 actatcaaaa atgctgacga aatttgtgtt cttcttgatg gtaaaatagt agaaagaggc
     7621 acacacgaag ctttgttgca agaaaatggt gtgtacaaaa aactgcaaga aacgcagaca
     7681 gtgtaataca caaaatcggt caaaacctaa cagtcaatat cgtaatgaga acgcaaataa
     7741 atcgttagtt cagcctctaa tttgtgccaa atcacgcata aaaaacctat ctttttctag
     7801 ggcattgcct actacaatca tatcagctcc agcaagatac gcatctttta cctgctctac
     7861 tgtgcgaatt cctccaccaa caataatcgg taatgaaatt gcttttctaa cagcttgaat
     7921 ggcttctttt gatacaggat tgatggctcc acttccagct tctaaataca ccatacgatt
     7981 gccaattagt tcgccagcta tggctgtggc tacaataatt tctactttat cgcatggaat
     8041 tggtttagtg ttactcatat attccacact ggtagaacat cctccatcaa taaggatata
     8101 ccccgtagaa atagattcga tacccgaatc gcgtatggct tttgccgcta ctacatgctg
     8161 accaattaaa aaatcagcat tgcgaccaga taacaacgac aacaacaata aagcatcggc
     8221 ttcgttgcac aattgatgac tatttccagg aaatagcacc acaggaatgt ttgtatgtaa
     8281 tttgatatac tgcactactt gctctacaga accagccacc aaactgccac ccaccataat
     8341 acaatcaggt aatgtttctt tagctaactc aacaaataat gcaagtgatt cttgagaata
     8401 tttatcagga tcgacaagta gtgctaactg cttttgattg actgattttt tatgtatact
     8461 atcttgataa aacactttat tttgagactt tgcgtaaaac catggctgta cgagatggga
     8521 gatagagttt aatccactct ttttcttccc ctttcacatt agttggtatg gtaaaatgtc
     8581 gaatactatc atcacataat ccaaacccac catattcttt tgcatctgta ttcaatacaa
     8641 cctcgtattc ccctttatct accaacatgc catagtctgt aaacgaatta tacggatgaa
     8701 aattaaatac aaacaataac tcaccaccac gtgcaaacgc aagcacctga tcgccagaat
     8761 gatcccaaca tttataaaca cctgaatctt gaaatttatc tgttttggtt aatatatcaa
     8821 tcatacctct atcaaacatt gccaaagagt gatataaata attagggtta tctaccaa
//
LOCUS       DAOSMY010000012         8786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_7956_length_8786_cov_3.702898, whole genome shotgun sequence.
ACCESSION   DAOSMY010000012 DAOSMY010000000
VERSION     DAOSMY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_8786_cov_3.702898"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="pta"
                     /locus_tag="PLD76_00605"
     CDS             complement(<1..898)
                     /gene="pta"
                     /locus_tag="PLD76_00605"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOU26539.1"
                     /translation="MKLLEKIFQQAKNNLRCIVLPESFEERTLRAADIVLQQQIAHIQ
                     LIGNKASILTTAKELGLAHIHQARFFDPETDPKMDEYSQLLYSLRKAKGMTLEEARVL
                     AKNPLYLSVLMIKNGDVDGELAGAANSTGNVLRPALQIIKTKPGVSVVSGVFFMLFDD
                     TSIGEDGVMVFADCAVTPNPSAIQLAEIAVVTAETTQIIGGFTPRVALLSFSTKGSAK
                     HEMVDKVVTATQLAQQQAPTICIDGELQVDAAIVPSVGASKAPNSLLKGNANVLIFPT
                     LETGNIAYKLVQRLAGAEAIGPV"
     gene            complement(984..1901)
                     /gene="xerD"
                     /locus_tag="PLD76_00610"
     CDS             complement(984..1901)
                     /gene="xerD"
                     /locus_tag="PLD76_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOU26540.1"
                     /translation="MIQNSNEHLVLRQYRSFLMLEKSFSDNTYQAYVHDVQKLLQFLE
                     DEKISYLTVTLVDLQHFLFQLAQIGINERSQARIISGIKSFYAFLVLENRLQDDPTSL
                     LESPKLPKKIPQILSIEEIDALIQAIDYSVPEGIRNRAIIETLYSCGLRVSELVELKL
                     SDIFWQEEFIKVVGKGNKERLIPISRTALKEIKSYLTERINLPIKKGSEDIVFLNRRG
                     NKLTRQMIFIIINKLADEIGLDKQIGPHTFRHSFATHLLEGGANLRVIQEMLGHESIL
                     TTEIYTHVDVHFLRETIMSYHPRNKKIKN"
     gene            complement(1913..3079)
                     /locus_tag="PLD76_00615"
     CDS             complement(1913..3079)
                     /locus_tag="PLD76_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU26541.1"
                     /translation="MMNPDSQFDDIRCYNDAEIPLQFSKLVEDKQLLAILPQILPNIP
                     VSVLIEQLKKVQSVEQFQKGFILPFLSDLETKTSKGVELIQLDRVNPHRHHLFISNHR
                     DIVLDSAFLNIHLLKNGYQTTEIAIGNNLLIYPWIEHLVRINKSFIVRRNASVREQIR
                     ISQELSAYIRYVITTKPHSVWLAQREGRAKDSDDRTQPALIKMLQMSAEGEYLKGIMD
                     LNIVPLSINYEHDPCDYLKAKEFQLKRDDECYKKSPADDLLNMSTGIMGYKGRISYVF
                     GTPLNDHPALNLVSQLSSKEQSTYIAHLIDTEIHRNYNLYPCNYVAADLLSKQNFFTK
                     HYSKEDEKDFIAYVQQRVNKIDISNKDEDFLTRKILEMYANPVYNQREAIRIQQ"
     gene            3153..3617
                     /locus_tag="PLD76_00620"
     CDS             3153..3617
                     /locus_tag="PLD76_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOU26542.1"
                     /translation="MAKEIERKFLVDKTKWQAVSKGMLLSQGYLSATENTTVRVRTTA
                     DKGFLTIKGKTQGFSRDEYEYIIPLQDAQEMLTMCSDKLVTKYRYTEKHNDMLWEIDV
                     FTDKNEGLIIAEIELTSEEQKFVLPDWVEKEVTGDIRYYNSFLAKTPFSTWE"
     gene            complement(3629..4075)
                     /gene="rpiB"
                     /locus_tag="PLD76_00625"
     CDS             complement(3629..4075)
                     /gene="rpiB"
                     /locus_tag="PLD76_00625"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU26543.1"
                     /translation="MKALDTLYIGIASDHAGYEMKERLKEYFAHTFFALKDFGTHSEE
                     SVDYPDYIHPLAQAVAKNEIDFGIAICGSGNGVSIVANKHKNVRAALCWTDEIAILAR
                     KHNDANIISLPARFISQALAEDMVERFLTTDFEGGRHCNRVNKINE"
     gene            4259..5350
                     /locus_tag="PLD76_00630"
     CDS             4259..5350
                     /locus_tag="PLD76_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOU26544.1"
                     /translation="MSDSISLYFNDVLVNPTLPSYMSENEYIASNLHTQDFSVALIGI
                     PDKNNSANTVRNHLYTLFGNFTTIKLRDLGNLKKGKSEKEMALALEHVVQELAEKSIP
                     CIVFGGEQWLTTAIFKGITEVQPNVGVCFIDSKIDVGDMQESSCSHSFITQLATHHTL
                     DNIDIVGAQQYYYTERQRQYIREHKGDITRLSEARKDIFKLEPTLRNADLISIDMSAV
                     RLSDAPAAKFPCPNGFTGEEVCQLTRFAGYADKTKALVISAWDCINDHQHHTSRLVAE
                     TIWHFLDGVDKREQESPNEDNPIYTKLVVQSDIHDDGIIFYHNTQTDRWWIEIPTLSG
                     LRIYPCHTNDYQALCNNEIPAIWLRYLYK"
     gene            complement(5353..6576)
                     /locus_tag="PLD76_00635"
     CDS             complement(5353..6576)
                     /locus_tag="PLD76_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU26545.1"
                     /translation="MKYLLIPPTALQGTVVLPTSKSISNRVLIINALAESDMPIEGVS
                     DCDDTRVMLNVLQSNDTAFDVGAAGTSMRFLTAFLSKIVGKWEITGSERMKNRPIKLL
                     VDALNELGAKIEYIEKEGYPPLRIYGSALQGGRIEMQGNVSSQYISALLMLAPTMQQG
                     LRLVLQGDIISVPYIQMTIKIMEQFGIQSYWKDNEIYIPNQAYTPVPFVVEGDWSAAS
                     YWYEMLALAPQGEVELKGLYKDSVQGDAAIATIFEHIGVKTKFTKQGVILSKKAPKKG
                     KLVYNFVNQPDLAQTCVVCCCLLGIPFYFSGLQSLKIKETDRIYALVTELKKLGYVVH
                     QKDNSILEFNNERCDVATEQSIDTYDDHRMAMAFAPACIKVGEISINEPHVVTKSHPN
                     FWVDLQSLGFTLHQM"
     gene            6759..8633
                     /locus_tag="PLD76_00640"
     CDS             6759..8633
                     /locus_tag="PLD76_00640"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOU26546.1"
                     /translation="MEEEIKNRHPEAEYTNDNIKTLEWQEHIRLRPGMYIGKLGDGSS
                     PDDGIYVLLKEVLDNSIDEFTMGNGKQIDVSIANGRVEVRDYGRGVPLESIVDVTSKM
                     NTGAKYDSKSFVKSVGLNGVGLKAVNALSSYFCIQSFRNGDTATAEFERGTIKTKSGV
                     VPNTVKKGNGTLILFEPDNTIFVGFVYQDEYIETLLKNYVYLNTGLTINYNGKKFYSK
                     NGLHDLLSDTITADTLYPIIHLKGEDIEIAFTHSNQYNDEYYSFVNGQHTTQGGTHLS
                     AFKEAITKTIKDFYNKGFESSDIRNGLVAAISVRVIEPVFESQTKTKLGSKDMEPNGV
                     SVYKYINDFIKKEVDNYLHKHAETAEILLRKIIDSEKERKAIAGVTKIARDRAKKANL
                     HNKKLRDCRIHYSDDKVGDKATESVIFITEGDSASGSITKSRDVNTQAVFSLKGKPLN
                     TFGLTKKIVYENEEFNLLQAALNIEDGIEGLRYNKIIIATDADVDGMHIRLLMISFFL
                     QFFPDLIKKGHVYILQTPLFRVRNKKETIYCYSEEERQEAVNKLGAKAEITRFKGLGE
                     ISPDEFKHFIGKDMRLDPVGLRKEDAVKELLAFYMGKNTPERQTFIIDNLIVEEDLEL
                     "
BASE COUNT         2872 a         1661 c         1457 g         2796 t
ORIGIN      
        1 tcactggacc aatagcttct gccccagcta aacgttgcac taatttatat gcaatatttc
       61 cagtttccaa tgtagggaat atcaatacat ttgcattccc ttttaacaaa ctgttgggtg
      121 ctttagaagc cccaaccgac ggaactatgg cagcatctac ctgcaactct ccatctatac
      181 atattgttgg agcttgctgt tgagctaatt gtgtagcagt tactactttg tctaccattt
      241 catgtttggc acttcctttg gttgaaaaac tcaacaaagc aacacgcgga gtaaaccctc
      301 cgatgatttg agttgtttct gccgttacaa cagctatttc tgctaattgt atagccgagg
      361 gattaggagt aacggcacaa tcggcaaaca ccatcacacc gtcttcacct atgcttgtgt
      421 catcaaatag cataaaaaat acgccagata caacacttac ccctggttta gtttttataa
      481 tttgtaatgc tggtcgcaat acatttcccg ttgaatttgc ggcaccagcc aattctccat
      541 ctacatctcc attttttatc atcaacaccg agaggtacaa aggattcttt gctaataccc
      601 gagcttcttc taacgtcatc ccttttgcct tacgtaacga atacaaaagc tgactatatt
      661 cgtccatctt ggggtctgtt tcgggatcaa aaaatctcgc ttggtgaata tgggctaaac
      721 ccagctcttt ggctgttgta agaatactag ctttattacc aattaactgg atatgtgcaa
      781 tttgttgttg caagacaata tctgcggcac gcaatgtgcg ttcttcaaat gattcgggca
      841 atacaatgca acgtaaattg ttttttgctt gttgaaatat tttttctaat aacttcatat
      901 aaaacacctt aggaataata aattgttttt tttaaataga agtaccaaag ttacaagaat
      961 aatagcattg aaacaagcat tttttagttt tttatttttt tatttctagg gtgatatgac
     1021 attatcgttt ctctcaaaaa atgcacatct acatgcgtat aaatttcggt tgttaatata
     1081 ctttcatgcc ctaacatttc ttgaataacc ctcaaattag ctcctccttc gagtaaatga
     1141 gtagcaaaag aatgccgaaa tgtatgtgga ccaatttgtt tgtctaagcc tatttcatct
     1201 gctaatttat taatgatgat aaaaatcatt tgtcgcgtta gtttattccc tcttctgttt
     1261 aaaaaaacaa tatcttcact cccttttttt ataggcaaat tgatacgttc tgttaaataa
     1321 cttttaattt ctttcaatgc cgtacgagat atcggtatca atcgttcctt attgcctttt
     1381 cctaccactt ttataaattc ttcttgccaa aaaatatcag aaagtttcaa ctccaccaat
     1441 tcagacactc gcaacccaca actatacaat gtttcaatta tagccctgtt tcttatcccc
     1501 tctggcacag aataatcaat ggcttgaatc aacgcatcaa tttcttctat tgataaaatt
     1561 tgcggtattt ttttgggtaa tttcggtgat tcaagcaaac tcgttgggtc atcctgtaat
     1621 ctattttcga gcaccaaaaa agcataaaaa gattttatcc ccgatataat acgagcctgc
     1681 gaacgctcgt tgataccaat ttgtgccaac tgaaataaaa agtgctgtaa atctaccaaa
     1741 gtaacagtta aataggagat tttttcgtcc tccaaaaatt gcaataattt ttgcacatca
     1801 tgcacatatg cctgataggt attatcagaa aaagactttt caagcattaa aaaagaccta
     1861 tactgacgta atacaagatg ttcattacta ttctgaatca cataagtatt ttttattgtt
     1921 gtattcgaat ggcttctcgt tgattataaa caggattggc atacatttcg agtatttttc
     1981 gggttaaaaa atcctcatct ttattcgaaa tatcaatttt attcactcgt tgttgcacat
     2041 acgcaataaa atctttttca tcttccttgg aataatgctt agtaaagaaa ttttgcttac
     2101 tcaataaatc agcagccaca taattacacg gatacaaatt gtaatttcta tgaatctctg
     2161 tatctatcaa gtgtgctata tacgtagatt gctcttttga cgaaagttga gagactaagt
     2221 tcaacgcagg atgatcattt aaaggagttc caaacacata cgaaatacgt cctttatagc
     2281 ccataatacc agtagacata ttcagcaaat catcagcagg tgatttttta taacattcat
     2341 catctcgttt aagctgaaat tctttggctt ttaaataatc acaagggtcg tgttcataat
     2401 tgattgacaa tggcactata ttcaaatcca ttatcccttt tagatactct ccctctgcac
     2461 tcatttgtag catcttaatc aatgctggtt gtgttctatc atccgaatcc ttagctcttc
     2521 cctctcgctg tgctagccat acagaatgtg gttttgtggt aattacatac cgaatataag
     2581 cagataattc ttgcgaaata cgtatctgtt cgcgtacaga ggcattgcgt ctaacaataa
     2641 aacttttgtt aatcctaaca agatgttcta tccaagggta aataagtaaa ttatttccaa
     2701 tggcaatctc tgtcgtttga tatccatttt ttagtaaatg aatatttaaa aatgctgaat
     2761 caagcacaat atctcggtga ttggatataa ataaatgatg tctgtgagga tttactctat
     2821 ctaactgaat caactctact ccttttgatg ttttcgtttc taaatcagat aaaaagggta
     2881 aaataaatcc cttttgaaat tgctctacag attgtacttt ttttagctgt tcgataagaa
     2941 ccgaaactgg aatattcggc aaaatctgtg gtagtatggc aagcaactgt ttgtcttcta
     3001 ctaatttgct aaattgcaag ggtatttctg catcgttata acaacgaata tcatcaaact
     3061 gactatcagg attcatcata gtattatttt cttttgccca aagatacaaa tttttagaaa
     3121 aacacgtaca tttgtatgca ataaatacga ctatggcaaa agaaattgaa agaaaatttt
     3181 tggtggataa aacaaaatgg caagctgtat caaaaggaat gttgctttct cagggatatt
     3241 tgtctgcaac cgaaaacact actgtccgtg tacgcacaac agcggacaaa ggttttttaa
     3301 ctatcaaagg aaaaacgcaa gggtttagtc gagatgaata cgaatacata atcccgcttc
     3361 aagatgcaca agaaatgcta acgatgtgct ctgacaaact agtaacaaag taccgttaca
     3421 cagagaaaca caatgatatg ctttgggaaa tagatgtttt tacagataaa aacgaaggat
     3481 taattatagc tgagatagaa cttacaagtg aagaacaaaa attcgtattg cccgactggg
     3541 tagaaaaaga ggtaacggga gacatccgct attacaactc atttttggct aaaacgccat
     3601 tttctacttg ggaataacat caatttcctt attcattaat tttatttact cggttacagt
     3661 gtctgcctcc ttcaaaatca gtagtcagaa aacgctctac catatcctcc gccaaagctt
     3721 gcgaaataaa acgtgctggc aatgatataa tgttcgcatc attatgcttg cgagctaata
     3781 tggcaatttc atctgtccaa caaagtgctg cacgtacatt tttatgtttg ttggcaacaa
     3841 tacttactcc attgccacta ccacaaatag caataccaaa atcaatttcg tttttagcta
     3901 ctgcttgtgc taatgggtgt atatagtcag gataatcaac actctcttcg gaatgagtac
     3961 caaaatcttt aagagcaaaa aacgtgtgtg caaaatactc tttcaatctt tctttcatct
     4021 catagccagc gtggtcgcta gcaataccta tatacaaggt gtctaatgct ttcatttttg
     4081 ttcaattgtt tgacgataca attatctatt taaaaaaact tgaataaccc aaccatattt
     4141 tactatgagg aatcatattg ttgttcagtt tgcaaaaaat atgtattttt gtttgctctg
     4201 caaagataca ttttttgcac acaacaattg actaaattta ctctaatttt ttacaagcat
     4261 gtccgactct atttccttat attttaacga cgtattggta aacccaacat taccatcgta
     4321 tatgtcagaa aatgaataca ttgcatcaaa ccttcacacg caagactttt cggtagcact
     4381 tattggcata cctgataaaa ataactctgc caataccgtc cgcaaccatc tgtacacact
     4441 ttttggcaac ttcactacta taaaactacg tgatttgggc aatctgaaga aaggaaaatc
     4501 agaaaaagaa atggcattgg cattggagca tgtagtacaa gagttggcag aaaaatctat
     4561 tccttgtatt gtgtttggcg gagaacaatg gctcacaact gctattttta aaggaataac
     4621 tgaggtgcaa cccaatgtag gggtgtgctt tatcgactcc aaaattgatg ttggcgatat
     4681 gcaagaatcg agctgttctc attcttttat cacacaactt gcaacacatc acacactaga
     4741 taatatcgac attgtaggag cccaacaata ttattacaca gaacgacaaa gacagtatat
     4801 tcgtgaacat aaaggagata taacccgctt atcagaagct cgtaaagata ttttcaaact
     4861 cgagccaact ctacgcaatg ccgatttaat aagtattgat atgagtgctg tacgtctatc
     4921 tgacgcacca gcggctaagt ttccttgtcc caacggattt acaggtgaag aagtttgcca
     4981 actaacacgt tttgctggct atgctgacaa aacgaaggct cttgttattt cagcatggga
     5041 ctgcatcaac gatcaccaac atcatacgtc acgtttagtt gctgaaacaa tatggcattt
     5101 cttggatggc gtagacaaac gtgaacaaga gtctccaaac gaagataatc ccatatatac
     5161 aaaacttgtg gtacaatcag acattcacga tgacggtatt attttttacc acaacacaca
     5221 aaccgaccgt tggtggattg aaatacctac cctttctgga ttacgtatct atccatgtca
     5281 tacaaacgat tatcaggctt tatgcaacaa cgaaatacca gcgatatggc ttcgttatct
     5341 atataaataa gcctacattt ggtgcaaagt aaacccaagc gattgcaaat caacccagaa
     5401 atttggatga gatttcgtta ccacatgtgg ttcgttgata gaaatttctc ctacttttat
     5461 acaagcagga gcaaatgcca tagccatacg atgatcatca tatgtatcta tgctctgttc
     5521 agtagccaca tcacaccgtt cattattaaa ctctaaaata ctattgtctt tttgatgaac
     5581 gacatatcct agctttttta attccgtaac caaggcatag attctgtctg tttctttaat
     5641 ttttaaactt tgtaaacctg aaaaataaaa aggtatccct aacaaacagc aacacacaac
     5701 acaggtttga gccaaatcag gctgatttac aaaattatat actagtttgc ccttttttgg
     5761 agctttctta gataagatta ctccttgctt tgtaaacttt gttttcacac caatgtgttc
     5821 aaaaattgta gcaatagcag catcaccctg cacactatct ttatacaagc ctttcaattc
     5881 tacctctcct tgtggtgcta atgcaagcat ttcgtaccaa tacgaagcag ccgaccaatc
     5941 tccttctacc acgaagggaa ctggtgtata agcttggtta ggaatgtata tttcattgtc
     6001 tttccagtat gattgaatac caaactgctc cattattttt atcgtcattt gtatgtatgg
     6061 caccgaaata atatcgcctt gcaacactaa ccgcaatcct tgttgcatag taggtgctaa
     6121 cattaacaaa gcagaaatat actgactact aacattccct tgcatttcaa tacgaccacc
     6181 ctgcaatgca ctgccataaa tacgcaacgg tggatatccc tctttctcaa tgtattcaat
     6241 ttttgctcct aactcattca gtgcatcaac caatagctta atgggtctgt ttttcatgcg
     6301 ttcgcttcca gttatttccc atttacctac tatcttagat agaaatgctg ttaaaaaacg
     6361 catagacgtg cctgctgctc ctacatcaaa tgcggtgtcg tttgattgca atacgtttaa
     6421 catcacacgc gtgtcgtcgc aatcagaaac accttcaata ggcatatcgc tttctgccaa
     6481 agcattaata atcaacactc tattacttat acttttagat gttggcaata cgactgtacc
     6541 ttgtaacgca gtgggcggga ttaacaaata tttcattctt ttattcctat gttttgttta
     6601 gtatggatga aagcgtttat actatctttc aaattgcaaa aatacacatt ttcaagcata
     6661 tttctttaac ccattatttt ttttaaaaaa gtacatcttt tcccatatct aaatctttcc
     6721 tttttattta aaaaaatgta cctttgtacg ttaaaattat ggaagaagaa attaaaaata
     6781 gacatcccga agctgaatac accaacgata acatcaaaac gttggaatgg caggagcata
     6841 tacgcttacg ccctggcatg tatattggta agctaggaga tggctcctcg cctgatgatg
     6901 gtatatatgt gctactcaag gaggtattag acaactcgat agacgaattt actatgggta
     6961 atgggaaaca gattgatgtt tccatagcaa atggacgggt tgaagtaaga gattacggac
     7021 gtggagttcc cttagaaagt attgttgatg taacctccaa aatgaataca ggagctaaat
     7081 acgactctaa gtcttttgtt aaatctgtgg ggttaaacgg tgtaggtcta aaagcggtaa
     7141 atgcattgtc ttcgtatttt tgcatacaaa gttttagaaa tggagatacc gcaacggcag
     7201 aatttgagcg tggcacaatt aaaaccaaat caggagttgt acccaacacc gtaaaaaagg
     7261 gcaatggaac tcttattctt tttgagccag ataatacaat ttttgtaggt tttgtatacc
     7321 aagatgaata catagaaacc ttattaaaaa actatgttta tttaaacaca gggcttacca
     7381 tcaattataa tggtaaaaag ttttatagta aaaatggatt gcacgattta ttaagcgata
     7441 ccattactgc tgatacgctt tatcctatta ttcacctcaa aggagaagat atcgaaattg
     7501 catttacaca cagcaatcaa tataatgatg agtattattc gtttgtaaac ggacaacata
     7561 ccacacaagg aggcacacac ctttcagctt ttaaagaagc tatcaccaaa acgataaaag
     7621 acttttataa taaaggcttt gaatcatcag atatacgcaa tggcttagtg gcagctatca
     7681 gtgtgcgtgt aatagaacct gtgtttgaat cacaaactaa aaccaagtta ggttctaaag
     7741 atatggaacc gaacggtgtg tcggtatata aatacatcaa cgattttata aagaaagaag
     7801 ttgacaatta tttgcataaa cacgcagaaa ctgctgaaat tttactcagg aaaattattg
     7861 attcagagaa agaacgcaaa gcaattgcag gtgttactaa aatagctaga gacagagcaa
     7921 aaaaagccaa tttacataac aaaaaattac gtgattgtcg tattcattat tcagacgata
     7981 aagttggtga taaagcaaca gaatctgtaa tttttattac cgaaggagat tctgctagtg
     8041 gatctattac taaaagtcgc gatgtaaata cgcaagccgt ttttagttta aaaggaaagc
     8101 cattaaatac ctttggttta actaaaaaaa ttgtgtacga aaatgaagag tttaacctct
     8161 tgcaagctgc tcttaatatt gaagatggta ttgaaggatt gcgatacaat aaaattatca
     8221 ttgcaacaga tgccgacgta gatggtatgc acattcgact attaatgatt agtttctttt
     8281 tgcaattctt tcccgatttg attaaaaaag ggcacgtata catcttgcaa acaccgcttt
     8341 ttagagtccg aaataaaaaa gaaacgatct attgttattc agaagaagaa agacaagagg
     8401 cagtaaataa attaggagct aaagctgaaa ttactcgatt taaaggattg ggagagatat
     8461 cgcctgatga atttaaacat tttattggta aggacatgcg tttagaccct gttgggctta
     8521 gaaaagaaga tgctgtgaag gagcttttag ctttttatat gggcaaaaat actcctgaaa
     8581 gacagacttt tattattgac aacctcatag tagaagaaga tttagaattg tagcattttt
     8641 tttgaaaaat atttttatac aacaagaatt attcgtttat ttgcgtaaca aaacatacca
     8701 caaaaaaaca cattatggat acattaatta aaaacctagg agttatcgtt attattttag
     8761 gagctttagc tctgattatt ccttct
//
LOCUS       DAOSMY010000013         8618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_8150_length_8618_cov_4.307486, whole genome shotgun sequence.
ACCESSION   DAOSMY010000013 DAOSMY010000000
VERSION     DAOSMY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8150_length_8618_cov_4.307486"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLD76_00645"
     CDS             <1..392
                     /locus_tag="PLD76_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOU26547.1"
                     /translation="KISKFDKVNHLLEAISYISLKRLFVVSALSLLRYTIYVSQYYFL
                     LLCFGVDISMYEAFVAIPINYLLVSITPSIAFAEMGIRGSYAIIIIGAFSHNPPAVAL
                     AGVTVWLINYVIPMITGSLLLTAKKKL"
     gene            479..2032
                     /locus_tag="PLD76_00650"
     CDS             479..2032
                     /locus_tag="PLD76_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOU26548.1"
                     /translation="MTNIHILWVDDEIDLLRSHILFLESKGYTVTTANSGHDALALCK
                     KETYDIAFLDENMPGLSGLETLSEIKKMQPALPIIMITKNEEEDIMEQAIGKKIADYL
                     IKPVNPNQIVMAIKKCLHSKQLISDKVTSSYQSAFTQIGMEINDTYTFEDWKKVYKHL
                     VYWELELEHADNQMDSLLMMQKNEANANFIKYIRKNYTSWFEPNDDKPLMSPDIFKNK
                     IFPLLDKGEKVCFVVIDNLRFDQWKVLQKELSQWFYTADDDLYCGILPTATQYARNAI
                     FAGLMPLQIQKMFLHYWIEEEDEEGKNMKEEELLQTQLDRFRKKYSFSYHKINTSQAG
                     EKLNEQIHQYLANDLNVVVFNFIDMLSHVRTESKIFRELVPDEAAYRSLILSWFKHSS
                     TNDFFKILAEKGIKVVLTTDHGTIRAKNPIKVVGDKNTNTNLRYKVGKNLSYNKKEVF
                     PITQPELIGLPSPNISSHYIFATQEDFFAYPNNYNYYVSYYKDTFQHGGISLEEMLIP
                     IAVLEPKRK"
     gene            2035..2937
                     /locus_tag="PLD76_00655"
     CDS             2035..2937
                     /locus_tag="PLD76_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOU26549.1"
                     /translation="MNTIPPFLKKGDTIAIISPSGVVDASFVYAAQEVLEKWGYKVVL
                     GKHCLAQDGRFAGTDSQRLSDLYWAFTNVDINAVLCSRGGYGLIRILSKLQETELFNQ
                     PKLLVGYSDITVLHNLLAKNSISSVHAPMAKHIAENPDDETSTFLCNTLAGVLPSFSI
                     PSHACNRIGNVKGKLLGGNLSVLYSLRGTPFDLDYTDSILVIEDIGERLYHLDRMLTN
                     FKLGGVFDKIKGLVVGQFSDMPSDELFPYSPYEIIQRAVAEYDFPVCCDFPIGHVQGN
                     YPVLLGAEYELSIQSTNTYLRPLC"
     gene            2931..3818
                     /locus_tag="PLD76_00660"
     CDS             2931..3818
                     /locus_tag="PLD76_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOU26550.1"
                     /translation="MLKAMFAHISAWKQFVVVVFLLLVAFFLSSTLFYVIASMRGVSQ
                     TFSSDINWLRFLQAFQSLMVFVLVPIVAGRMFSFNIKSYYIFRKPTAAQILWVIVLMS
                     SLIPLINISLQLNKLVVFPESMQSLYTWMLQLEQQATDVLQFFFSDTSVLGLVFSLFI
                     IAVIPAIGEELVFRGLLQKILTQWTKNAHIGIWISAFLFSFIHFQFFGFLPRMLLGAF
                     FGYLFLWSGNLLIPMVAHFVNNAIGVTMYWLYYRNSIDINIDTIGVEEHSMILTIFSI
                     IFVGLLFYFSPFRTKNPVA"
     gene            complement(4365..5321)
                     /locus_tag="PLD76_00665"
     CDS             complement(4365..5321)
                     /locus_tag="PLD76_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU26551.1"
                     /translation="MKKYLVIAILSLTSIVSLSAQEEIVYNQYHFNYYLANPALAGAE
                     PCSHFMLTNKFQWVGMDEFPMTQTLSFRTRALDRIGIGAFLYNDRNGYSNRMGGQATF
                     AYHIPLSDGRRYRQNVSLDRQLSFGVSFKLNYFSINQELLDDPSTQGDNAFANPDGLA
                     PNANFGVYFKSYGFFTGLSLTNLIPTTPDIFGDKEVPAPFTGFFFLGNAFELDYDAFI
                     EPSIMLKANQYGNIGGDINLKFGQEVDRDFGYWIQASYRQSFDRDNGQSLVLMPMGGV
                     RFGKFQLGYSFGLDLNNLVTHSYGSHEIMLGYSFCIPKYFCR"
     gene            complement(5403..>8618)
                     /locus_tag="PLD76_00670"
     CDS             complement(5403..>8618)
                     /locus_tag="PLD76_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOU26552.1"
                     /translation="FTDLCPNTIYDFSAFVANATTENGQTRAPVNVGFRVFGCNGTDC
                     TTQLIKVESGDLPSSTNWVEKGGSFNSQGFSSVKVQLFNNLDSKPYAIKEKDGTLKVI
                     GNDIAIDDITFSTCSPEIQVFSNAEYTKKDQTFLAENTERVYVWAPYDLTEFFATPWY
                     VIQESLDGTSDWTNIYSGHNDYKDVDITLVKYLDKKYNYRAFVAADQETAEARAANPK
                     ILKTGCGQISAVSDTLWLTMEKPCTATPPPSVTNYDECPLTGTGTLATLVTSDTTQLR
                     WYTQDTGGTALTTTTFDWTTVDFYTLYVTNQLADQGTTTYCESTRTAVTINIKPGVTF
                     KVDKKEINVCTNSTDAERTFTVINKLPATAAITWKAMSDESFLGTGNTYTVPATVGQG
                     ELIIDGTDPTGCDFRDTVRYQVKALPDFTLLAPTLVCVSDPKATLTATITTDENGSTY
                     AWYKGTSTTPVGSGTLTNETSITHQDNSVGTTQGNVTYRLVINNGICQTPKTVTIAVG
                     TEVNIPIISSDTNNAICAGDLVDLTAQITLNTGETLLWKKDGVEMTETGTTITGQDLT
                     ANTVYTVELQGGSCDGDGTITITVSTPITPELEISSDVICLGGTINIEDINTENAASY
                     VWESRVGTGNWTTMSQTSKNLTGIAPEVTTTYRKTAINGECTRVSNEVTVRVEPPLNF
                     DVSNDITICAGSTTTLTMSNYPEEAVTFWIEGDINADDFMNAFKDAFVGETTNLLEAL
                     MNMMFSSLDDIVSDGNGTENPVVEDDDLTNEDELPLIDESFFEEIFTSLGIIGMEPTI
                     DVTPTTTTTYTAVVMGVCTATRQVTVTVDAPITASISAPTTICVGDNTQLVATGGTTY
                     RWTPATGLNNPNISNPIASPSVTTVYSVEITRGTCSDVTETTVTVVPLPEITNIEETN
                     VREVTAYVENGLPPYSYSINNRDFITHNVLHDVSIGGHILYVVDMNNCKTSRPFEVTP
                     IPIFPAKFFTPNDDGVNDYWTIENLNVYPSYIVQIFDRFGKLIYEVRSGQFHADGEVN
                     YHPFQWDGNYLGHPLPSTDYWYLITLEEVRKQYTGHFTLKR"
BASE COUNT         2546 a         1483 c         1659 g         2930 t
ORIGIN      
        1 caaagattag taaatttgat aaagtgaatc atttgcttga agctatatcc tatatttctc
       61 taaagagact ttttgttgtt tcagcacttt ctttacttag gtatacgata tatgtttctc
      121 agtattattt tttattattg tgtttcgggg tagatatttc aatgtacgaa gcttttgtcg
      181 ctataccaat taattatttg cttgtatcta taacaccttc aatcgcattt gccgaaatgg
      241 gcattagagg gtcgtatgct attattatca taggagcttt ttcacacaat cctcctgcag
      301 tagctttggc tggagttacg gtttggctaa taaattatgt tattcccatg ataactggtt
      361 cacttctttt aaccgcaaaa aagaaacttt aaccagataa tactgcttaa ataattgaag
      421 aatacctgta aatgtcatat ctttgtaaaa aagaacaaat caccaaacgt aaccatttat
      481 gacaaatata catattttat gggtagatga tgaaattgac ttattgcggt ctcatattct
      541 atttttagag tctaaaggat atacggtaac tacggctaat agtggacatg atgcattggc
      601 attgtgcaaa aaagaaacgt acgatattgc tttcttagac gaaaatatgc caggattaag
      661 cggtcttgaa accttgtcag agataaaaaa aatgcaacca gcattgccta ttatcatgat
      721 aacgaaaaat gaggaagagg atattatgga gcaggcaatt gggaaaaaaa ttgcagatta
      781 cttaataaag cctgttaatc ccaaccaaat tgtgatggca attaaaaaat gtttgcattc
      841 caaacaatta atttcagata aagtaacgag ttcataccag tctgctttta cccaaatcgg
      901 tatggagata aatgatacgt atacattcga agattggaaa aaggtttaca aacatttagt
      961 gtattgggag ttggagctag agcatgcaga taatcagatg gattcgttgc tgatgatgca
     1021 aaagaatgaa gccaatgcaa attttataaa gtatattaga aagaattaca catcgtggtt
     1081 tgagcccaat gacgataaac ctttgatgag tccggatata tttaagaata aaatatttcc
     1141 attgttagac aaaggagaaa aggtatgctt tgtggtcatt gataatttgc gtttcgatca
     1201 gtggaaagta ttgcaaaaag aattgtctca atggttttac actgcagatg atgatttgta
     1261 ttgtggtatt ttacctactg ctactcaata tgcacgaaat gcaatttttg cgggattaat
     1321 gcctttgcag attcaaaaaa tgtttctcca ctattggata gaagaggagg atgaggaggg
     1381 gaaaaatatg aaggaagaag agttgttaca aacgcaactt gatagattta gaaaaaaata
     1441 cagtttttcg tatcataaga ttaatacttc gcaagctggc gaaaaattga acgaacagat
     1501 tcatcagtat ttggcaaatg atttgaatgt ggtggtattt aattttattg atatgttatc
     1561 gcatgtgcgt accgaatcca aaatttttag agagttggtg cccgatgagg ctgcatatcg
     1621 gtcgcttatt ttatcgtggt tcaaacattc ttctaccaat gattttttta aaatattagc
     1681 agaaaagggt attaaagtag tccttacgac cgaccatggt actatacgtg caaaaaatcc
     1741 gattaaagtg gtaggagata agaatacaaa tacgaattta cgctacaaag taggaaaaaa
     1801 tttaagctac aacaaaaaag aggtatttcc tataacccaa ccagaactca ttgggttgcc
     1861 tagtcccaat atcagttctc attatatttt tgctacgcaa gaagattttt ttgcctatcc
     1921 gaataactat aattattacg tcagttatta caaagatacg tttcaacatg gtggtatttc
     1981 tttggaagaa atgctcattc caattgcggt attagaacct aaacgaaaat aacgatgaac
     2041 acgattcctc catttttaaa aaaaggcgat actattgcta ttatatcccc atcaggtgtt
     2101 gtggacgcta gtttcgtgta tgctgctcaa gaggtgttag aaaagtgggg atacaaagtg
     2161 gttcttggta agcattgttt agctcaggac ggtcgttttg ctggaacaga tagccaacgc
     2221 ttatctgacc tttattgggc ttttaccaat gtagatatta acgctgtttt atgcagtaga
     2281 ggtggatatg gactgattcg tatcttgtcg aagttgcaag agacggaatt gtttaaccaa
     2341 ccaaaattat tagtaggata tagcgatatt accgttctac acaatctgtt ggcaaagaat
     2401 agtatcagtt cggtgcatgc acctatggcg aaacatatag ctgaaaatcc agatgatgag
     2461 acgagtacat tcctgtgtaa tacgctcgca ggagtgttgc catcgttttc aattccatct
     2521 catgcatgta acagaattgg caatgtgaag gggaaattgc ttggaggaaa cttgtcagtg
     2581 ctctatagtt tgcgtggtac accattcgat ttggattata ctgatagcat tttagtaatt
     2641 gaagatattg gcgagcgttt gtatcatttg gatagaatgc ttactaattt caagctagga
     2701 ggtgtttttg ataaaattaa aggcttggtt gtgggtcagt tttccgatat gccgagcgat
     2761 gagttgtttc cgtattctcc atacgaaatc attcaacggg cagtagcgga gtatgatttt
     2821 cctgtatgtt gcgattttcc tattggtcat gtacaaggta attaccctgt attattgggt
     2881 gctgagtacg aattatctat tcaatcaact aacacatatt tgcgtccgtt atgttaaaag
     2941 caatgtttgc tcatatttct gcttggaaac aatttgtggt ggtagtgttt ctacttttag
     3001 ttgctttttt tcttagttct acattatttt atgtgatagc tagcatgaga ggtgttagtc
     3061 agactttttc gtcggacatt aactggttgc gatttttgca agcctttcag tctttgatgg
     3121 tttttgtgct tgtaccgatt gttgcaggga gaatgtttag cttcaatata aaatcatatt
     3181 atatttttcg gaagcctact gctgctcaaa tcttatgggt tattgtgttg atgagttcgc
     3241 ttataccgct tattaatatt agtttgcaac tcaacaaatt agttgttttt ccagaatcaa
     3301 tgcagtcatt atatacgtgg atgttgcaat tagaacaaca agcgacggac gtgttgcagt
     3361 ttttcttttc tgacaccagt gttcttggat tggtattctc tttgtttatt attgcggtca
     3421 ttccagctat tggcgaagaa ttagtgttta gaggcttatt gcaaaaaatt cttacgcagt
     3481 ggacaaaaaa tgctcatatt ggtatttgga taagtgcgtt tttgtttagt tttattcatt
     3541 ttcagttttt tggatttttg ccccgtatgt tattgggagc cttttttggc tatttattcc
     3601 tatggagtgg aaatttgcta attcctatgg tggctcattt tgtaaataat gctattgggg
     3661 taacaatgta ttggttgtat tatagaaata gcatagatat aaatattgat actattggtg
     3721 tagaagagca cagtatgata ctaacaatat tctcgattat ctttgttggt ttgttgtttt
     3781 atttctctcc ttttcgcacg aaaaatccag ttgcgtagaa atattattcc acagagttta
     3841 aaattttttt tcgtgtattt tcgtaaaaaa aacattacac agatagacac agaggattca
     3901 cagagatagg taatttatta aaaatataac gctatttgcg tggtaatatg tcgctcctac
     3961 ggagcttgca ttacccaatc tgtgcttatg ctattattat ttcgctccgc tggagctggg
     4021 ttttacttat tttcaagcaa aaatcgctat ttctcatgcc cagttagggg ctacatatta
     4081 atagaaaaaa catatcaata taaagagagc tccagcggag cggaatagat atctttgttt
     4141 gattttctct aggttatagt tatcgtactg agttgtaaat ctttgttcgt gttcttcgtc
     4201 aaagattttt acacgaagtt ccacagagga ttcacagagt ggcacggagg tttaaatttt
     4261 ttacttcgtg tatcttcgtg aaagaatttt tacacagaga cttgattaaa aaaagccccg
     4321 aaaacttttt acagtaatcg gggctttggt gtatcagtat tttcttatct acagaaatat
     4381 tttggaatac agaaagaata tccaagcatt atttcgtgcg aaccgtagct atgcgttacc
     4441 aagttattta agtctaggcc aaatgagtat cctaattgga atttgccgaa acgtacacca
     4501 cccattggca ttagcacaag actttgtcca ttatcgcgat cgaaactttg acgatacgat
     4561 gcttgaatcc aataaccaaa atctctatct acttcttgac caaattttaa attaatatcc
     4621 cctccaatat tcccatattg atttgccttt agcataatag atggttcgat gaaagcatca
     4681 taatctagtt caaaggcatt tcctaagaaa aagaatcctg taaatggagc aggcacttct
     4741 ttatcaccaa aaatatcggg tgttgttggg attaaatttg taagagatag accagtaaag
     4801 aaaccatatg atttaaagta aacaccaaaa ttagcatttg gcgcaagtcc atcaggattg
     4861 gcaaaagcat tatccccttg tgtgctaggg tcgtccaata attcttggtt aatgctaaaa
     4921 taattcaatt taaatgatac cccaaatgat agctgtctat ctagggatac attttgacga
     4981 tatctacgac cgtcacttaa tgggatgtga taggcaaatg tagcttgccc tcccattcta
     5041 tttgaatatc cattacggtc attatacaaa aatgccccta ttcctattct gtccaatgcc
     5101 ctagtacgga aacttaatgt ttgtgtcata gggaattcat ccatccctac ccattgaaat
     5161 ttatttgtta gcataaaatg tgaacaaggc tcagcaccag ccaatgcggg attagctaaa
     5221 taataattaa aatggtattg gttgtacacg atttcttctt gtgctgacaa ggatacaata
     5281 cttgtcaaac ttagaatcgc aataactaga tattttttca tatgttcaga ttaaaaatag
     5341 gtctctttcg ttttctaaaa aatatttatg tgccaaagat acaattttca ttgatatttc
     5401 tattatcttt ttagcgtaaa atgtcccgtg tattgttttc ttacttcttc taaagtaatt
     5461 aagtaccaat agtctgtgct tggtaatgga tgtccaagat aattaccgtc ccattggaat
     5521 ggatgataat taacttcgcc atctgcatgg aattgtcctg aacgcacttc gtaaattaac
     5581 ttgccaaaac ggtcaaatat ttgtacaata tacgatggat aaacgtttaa gttctctatt
     5641 gtccaataat catttacgcc atcatcgttg ggtgtaaaga attttgctgg gaatattgga
     5701 ataggtgtta cttcaaacgg ccttgatgtt ttgcaattat tcatatcaac aacatatagt
     5761 atgtgcccac ctatgcttac atcatgtagc acattatgtg ttatgaaatc tctattgtta
     5821 atggaatagg aataaggagg taagccattt tccacataag cagttacttc tctcacattg
     5881 gtttcttcaa tgttggtaat ttcaggaaga ggaacaacag ttactgtcgt ttctgttaca
     5941 tcagagcaag ttcctcgtgt aatttctacc gaatatacag tagtaacact tggcgatgct
     6001 attggattgc ttatatttgg attgtttaat ccagtagctg gagtccagcg ataggttgta
     6061 cctccagtag caacgagttg cgtgttgtct cccacacaga tggtagtagg ggcagatatg
     6121 cttgctgtaa ttggagcgtc gactgttact gttacttgac gcgttgctgt acatactccc
     6181 ataactacag cagtataagt agtggtggtg gttggtgtta cgtctatcgt aggttccatt
     6241 ccaataatac ctaagctagt aaatatctct tcaaaaaagc tttcatctat taaaggtaat
     6301 tcatcttcat ttgtaaggtc atcatcctct acaactgggt tctctgtgcc atttccatca
     6361 gaaactatgt catctaatga tgagaacatc atattcatca aagcctctaa aaggttagtc
     6421 gtttcaccca cgaaagcatc tttaaatgca ttcataaaat catcagcatt tatgtctcct
     6481 tcaatccaaa atgtaacagc ttcttctgga taattactca tagtaagagt tgtagtagaa
     6541 cctgcacaaa ttgtaatgtc gttacttaca tcaaaattca aaggaggttc aactctcaca
     6601 gtaacttcat tgctcacacg tgtacattct ccattaatag ctgttttgcg ataagtagta
     6661 gtcacttcag gtgcaatgcc agttaagttt ttgcttgttt ggctcatggt tgtccagttg
     6721 cctgttccta cacgagactc ccacacataa ctagcagcat tttctgtgtt gatatcttct
     6781 atatttatcg ttcctcctaa gcagataaca tcactcgaaa tttctaattc gggtgttata
     6841 ggagtactaa cggtaattgt aatagtacca tctccatcac aagagcctcc ttgtagttct
     6901 actgtataca ctgtatttgc agttaaatct tgtccagtaa tcgttgttcc tgtttctgtc
     6961 atctctacac catctttttt ccacaataat gtttcgccag tgtttagggt aatttgggca
     7021 gttaaatcta ctaaatcgcc cgcacaaata gcattattgg tatcagaaga gataattggt
     7081 atatttactt ctgtaccaac tgctatggtt actgtttttg gtgtttggca aataccatta
     7141 ttaattacca agcggtatgt aacattacct tgagtcgtgc caacagagtt gtcttgatgt
     7201 gtaatgcttg tttcatttgt taatgttcca gagccaactg gggtagtact agtccccttg
     7261 taccaggcat aggtacttcc attttcgtcg gtagtaatag tagctgttag tgtagcttta
     7321 gggtcgctca cacatacaag cgtaggtgcc aaaagcgtaa aatcaggcaa agctttgact
     7381 tggtacctca ctgtatctct gaagtcacaa ccagtagggt cagtgccatc tatgattagt
     7441 tcgccttgac caacagtagc aggtacagta tacgtattgc ctgttcctaa aaatgattca
     7501 tcactcattg ctttccatgt gatagcggct gtagctggaa gtttattgat tacagtaaag
     7561 gtgcgttcag catctgttga attggtacac acattaattt cctttttatc taccttaaat
     7621 gtaacacctg gtttaatgtt gatagtaaca gctgtacgtg tgctttcgca ataagtcgta
     7681 gtgccttgat ctgccagttg gtttgttaca taaagagtgt aaaagtcaac tgttgtccaa
     7741 tcaaatgtgg tagtagtaag tgctgtccct ccagtatctt gtgtgtacca tctaagttgt
     7801 gttgtatcac tggttaccaa agtggctaag gtacctgttc cagttaatgg gcattcgtcg
     7861 taattagtta ctgatggagg aggtgttgcc gtacaaggct tttccattgt taaccataat
     7921 gtgtcagaaa ctgcagaaat ttgtccgcaa ccagttttaa gaatcttagg attagctgcc
     7981 cttgcttctg ctgtttcttg gtcagctgct acaaatgctc tatagttata ttttttatct
     8041 agatacttaa ctagtgtgat atctacatcc ttatagtcgt tgtgcccgct ataaatattt
     8101 gtccaatcac ttgttccatc gagagactct tgtattacat accaaggagt tgcgaaaaac
     8161 tcggtaaggt cgtaaggagc ccatacatat accctctctg tattttctgc taaaaatgtt
     8221 tgatcctttt tagtatattc tgcatttgag aatacttgta tttcaggaga gcaggtacta
     8281 aaggtgatat cgtcaatggc aatgtcatta ccaataacct ttaatgtacc atccttctct
     8341 tttatagcat acggtttaga gtctaagttg ttgaatagtt gtacttttac actggaaaat
     8401 ccttgagaat taaacgaccc tcctttttct acccagttgg tgcttgaggg taagtctcct
     8461 gattcaacct ttattaattg tgtagtacaa tcggttccat tacagccaaa taccctgaat
     8521 ccaacattaa caggggctct tgtttgtccg ttctcggttg tagcattcgc aacgaaagca
     8581 gaaaagtcgt aaatcgtgtt gggacataaa tccgtaaa
//
LOCUS       DAOSMY010000014         8247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_8637_length_8247_cov_3.426025, whole genome shotgun sequence.
ACCESSION   DAOSMY010000014 DAOSMY010000000
VERSION     DAOSMY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8637_length_8247_cov_3.426025"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLD76_00675"
     CDS             <1..864
                     /locus_tag="PLD76_00675"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOU26553.1"
                     /translation="NTLVSIWQYLSENGATRKSLMINLGGGMITDLGGFAASTFKRGM
                     NYVNVPTTLLAMVDAAVGGKTGINFNGLKNEVGVINPAKSVLIDTNFLRTLDMQNLLS
                     GYAEMIKHALISSENELKNIFELDFAQLDYEKLNQLVQSSIYIKEAIVTEDPQEEGVR
                     KALNLGHTIGHAFESYSYQMNKPALHGYAVAWGLMCELFLSYKKLGFPKETLIKVARF
                     VKENYGHFYISCDDYDALYELMLHDKKNTIANCINFSLLSNVGVLSLNQHIDKETIFE
                     SLDFFQEMMGM"
     gene            891..2384
                     /locus_tag="PLD76_00680"
     CDS             891..2384
                     /locus_tag="PLD76_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOU26554.1"
                     /translation="MKSYLQHIPLGTSLPVSNPHAISVSFPSMDDVIGYEKKDECILQ
                     QLQSGYPRFFRNKHVHLLCETVRKEFKLPVSVEIYPLPCNAVLSLLNLYFCKDFVKID
                     AYGVTFLLLDENDVQLGKLRGFLRQVGVFMSSRNAEDILHSNGLISTTFNEAYTEVQP
                     YYFVQQSLQKAYETKSVDDIILSNCGMNAFFAVFQTIQDISLLSGKNRVLQLGNLYLD
                     SYDILRTYANKITLSHVTDFNEIRRMIEMHHTVLSAVYVEVPNNPLLECVDLPRLYAL
                     CQEYNIVLLVDSSLASAHTLKLLPYCDVITESLSKFANGQGDVLMGAIVVNAASTYSK
                     EIKSRIATYVIPPYVRDVQRLAHSISDYYERVCLSSASTRKLIEYLENSPEVEQVYSV
                     LHPLSKRNFSILQKNDSLIPSVFSVLFKQPLAYYYDKLPLPKGPSFGTVFTLAMPYVY
                     LAHFDLIQTVEGRQQLTEMGLHPDMLRISVGLEPVEDIIASFKLAGI"
     gene            complement(2403..3161)
                     /locus_tag="PLD76_00685"
     CDS             complement(2403..3161)
                     /locus_tag="PLD76_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOU26555.1"
                     /translation="MKKIKEFINNSRQNIHSHFRKTYLYKNTNPDLVKHLKPTITCPK
                     KWYGTSYGGFYINPNLLTNQSVIYSFGIGKDISFDMACSNHHQSSIYAFDPTPKSIKW
                     IKEQKLPANFHFFDFGISVGDQEFAAFYLPKNTKSVSGSTIQSDVFDTNNSIKLPMKS
                     FAKITEMLHHTHIDVLKIDIEGSEYEVLEDILTTKISINQLLIEFHDRLFDMQTYKSV
                     EIVKKLNEAGYHIFGCSKSFEEVSFIHASVLKNT"
     gene            complement(3175..4083)
                     /locus_tag="PLD76_00690"
     CDS             complement(3175..4083)
                     /locus_tag="PLD76_00690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU26556.1"
                     /translation="MPTISVIITTFNGSKNINNTLRAILQQEGINSLFELDIIAIDDC
                     STDNTLEILNEYPIRIFSTETNSGGPNKGRNIGLANAKGDYICIVDQDDVWHTNRISS
                     VLPYIQKAPIISSGYTLIDTSTGKTIERVAQSKEPFIFFEKNSTFLSKLTRSSQGQNT
                     YLGSLLFHRSLRNILFEEHFGKIDYDWVLRLFHQQNSIEICSSLYTRIVDGTNLSLNP
                     TYRRQDFYYSLYFIEQFENLYPTQTRIARKKIHGSRARYYYIMNDMRRARVYFRLAGF
                     SFNNILYYCTTFAGSSFVKKQFNVFG"
     gene            4323..5126
                     /locus_tag="PLD76_00695"
     CDS             4323..5126
                     /locus_tag="PLD76_00695"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU26557.1"
                     /translation="MVKYIDLDVAIQNSNSAFFKKMPKWVISLLKKIIHQDEMNRILN
                     KYEDCYGVDFLPKIIEELQLTVQIEGEENLPADGRCFFAANHPFGFVDGLILTNTVGK
                     KYGKLKAIGNDSFLLAPHLKPIIAAVNVFGSNPKAYLLELENLFESDSPITHFPAGVV
                     SRIRNGKIEDKDWQKSFIKQSVKYNRPIVPFHFTGRNSYLFYIIYLIRSFLGIKLNIE
                     LMLLPYEIFNKKGKTIHVKIGKPIMPETFDNSKTSAEWAQYVKQQVYSL"
     gene            complement(5128..5934)
                     /locus_tag="PLD76_00700"
     CDS             complement(5128..5934)
                     /locus_tag="PLD76_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOU26558.1"
                     /translation="MKKIIWLLVISFGITSCTEIIQLDLQNNKSEVVVEASMAAANEI
                     RVLLSRSVNLDESNTFPLLTGAQVSISDENNNTEVLTEIEPGVYQSITTKGIEGKRYF
                     LDLIVNNQPISATSTMPSIVPFTDMVIEKGSVFGPIGGNTNTDNTEYYEIKVLFNDPV
                     EEVNYYRFVEFINNEKQQEYIYDDRLNNGKDVEIVLFRFDRLLKTEDVVRIEMQTITK
                     EFYVYLSSLNDLRNSARSGSTPANPVTNMQGTSLGYFNTYTSQSKEYIIE"
     gene            complement(5934..8240)
                     /locus_tag="PLD76_00705"
     CDS             complement(5934..8240)
                     /locus_tag="PLD76_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU26559.1"
                     /translation="MCVITLLFLSHISVVAQQVFSISGTISDKATGETLTGATIYPKS
                     NPSSAVYSNAYGYYALSLAKGTHTIVVNYIGYSSLEYSIEVQENIRKNFQIQPASTQL
                     DEVVVSAIKRNETILSDKIGVEKLEIQSIQKIPVLFGEQDILKTLTLTPGVKTMGEGS
                     GGMHVRGGNNSHNLILLDESMVYNANHLLGFFSTFNGDAIKDVTLYKGTAGAEYGGRL
                     ASVMDVRMKDGNNQHYHVSGGIGLISSRISVEGPIIRNKGSFLLTARRTYADLFLKLS
                     SDPLLNNNQLYFYDINAKANYQLNDNNRLFISAYAGRDVFSFFDRFSMNWGNITATVR
                     WNHIFNNRLFSNTSFIYSDYGYKVGINLAENSFSLLSQINNVQLKHDYQYFLTAQNTL
                     SFGIQATHHRITPGQLSTESTLFQPFKLQEKYAFESSLYITNNWKPNYHWNVSYGVRG
                     NVFSLLGGGDFYTYLNDGTIQTSFYDYNQFVKTYLYIEPRINIAYILNDANSFKMSYT
                     RNTQNLHLITNSTSSVPTDIWIPSSKNIKPQISDQVSTGYFVHFLDDTYQVSSEIYYK
                     WMQNQLDLKNGANIQANEYIENELLVGNGRAYGLEIMLKKQYGALSGWIGYTLSRTEN
                     QIEGINNGNWYPARHDATHDVSVVAIYELSKKWTLSSTWVYNTGNAVTFPSGKYQVDG
                     ETILLYTNRNASRMPHYHRMDIGATRYFKKSKRFESSLNFSVYNAYGRKNAFTIDFEQ
                     DENDPDKINAVKTYLFTFMPSITYNFKF"
BASE COUNT         2454 a         1329 c         1567 g         2897 t
ORIGIN      
        1 aatacgctag tttctatttg gcaatattta agcgaaaatg gtgctacacg aaaatcgttg
       61 atgataaatt taggaggagg tatgataacc gatttgggtg gatttgctgc ttctacgttt
      121 aaaagaggta tgaactatgt aaatgtgcct actaccttat tggctatggt tgatgctgca
      181 gttggaggta aaacgggtat taactttaat gggttgaaga atgaagttgg ggtaattaat
      241 cccgctaaga gtgtgttgat tgatacaaac tttttgcgta cgcttgatat gcagaatttg
      301 ttatctggct atgcagaaat gattaaacat gcattgatat cttcggaaaa tgaattaaag
      361 aatatttttg aattagattt tgctcaattg gattacgaaa aactcaatca gttggtgcaa
      421 tcttctatat atattaaaga agccatagta acagaagacc cacaagagga aggtgtacgt
      481 aaagccttaa atttaggaca tactatcgga catgcttttg aaagctattc gtatcaaatg
      541 aacaaacccg cattgcatgg ttatgctgta gcttggggtt tgatgtgcga gttgttcctt
      601 tcgtataaaa aattaggctt tccaaaagag acattgatta aggtggctcg ttttgtgaaa
      661 gaaaattatg gacattttta catttcgtgt gatgattacg atgcattgta cgaactaatg
      721 ctgcacgata aaaagaatac aatagctaat tgtataaatt ttagcctatt atctaatgta
      781 ggggtgttgt cattaaatca acatatcgat aaagaaacga tttttgagtc gctcgacttt
      841 tttcaagaaa tgatgggtat gtaatttcct atacaagagg tttttataca atgaaatcat
      901 atttacaaca tatacccttg ggtacttcgt tgcctgttag caatcctcat gccatttctg
      961 tgtcttttcc tagcatggat gatgtaattg gttacgaaaa gaaagatgag tgtatccttc
     1021 agcagcttca atcaggatat cctcgttttt ttagaaataa gcacgtgcat ctgctttgcg
     1081 aaacagtacg taaagagttt aaattgcccg tgagtgtaga gatatatcct ttgccatgta
     1141 atgctgtatt atcattgcta aatttgtatt tttgtaaaga ttttgtaaag atagacgcat
     1201 atggagttac atttttatta ctagatgaaa atgatgttca gctaggtaaa ttgcgagggt
     1261 ttcttcgcca ggtgggtgtc tttatgtctt cgcgtaatgc ggaggatatt ttgcactcca
     1321 atggattaat ttcaacaacg tttaacgaag cctatacaga agtacagcct tattattttg
     1381 tacaacaatc ccttcaaaaa gcttacgaaa caaagtctgt agacgatatt attttgagta
     1441 attgtggtat gaatgctttt tttgctgtat ttcaaactat tcaggatatt agtttgttat
     1501 ctggtaaaaa tagggtattg caattgggta atctatacct tgattcgtat gatatattac
     1561 gcacctatgc taataaaatt accttgtctc atgtaaccga ttttaacgaa ataagacgaa
     1621 tgattgaaat gcatcacaca gtgttaagtg cggtgtatgt agaagtgcct aataatccct
     1681 tgcttgagtg cgtagattta cctcgtttgt atgccttgtg tcaagaatat aacattgttt
     1741 tattggtcga ttcttctttg gcttctgccc atactttgaa acttctgcca tattgtgatg
     1801 taataacaga gagcttatcg aagtttgcca acggacaagg agatgtgctg atgggagcta
     1861 tagttgtgaa tgcagcgagt acgtatagta aagaaataaa atctcgaatt gcgacttatg
     1921 ttattcctcc ttatgtgcga gatgtgcaac gattagccca ttcgatttct gattattacg
     1981 aaagggtatg tttgtcatcg gcaagtacac gtaaattgat tgagtatttg gaaaattcgc
     2041 ccgaagtaga gcaagtatat tccgttttgc acccattgtc taagcgtaat ttttcgattc
     2101 tacagaaaaa tgactcgctt ataccaagtg ttttttctgt tttgtttaag caaccattag
     2161 cctattatta cgacaaatta cctttgccca aaggtccaag ctttggtaca gtctttactt
     2221 tagctatgcc ttatgtatat ttagcccatt ttgatttaat acaaacagta gagggcagac
     2281 agcagttaac ggagatggga cttcatcctg acatgctacg aatatctgta ggattagaac
     2341 ctgttgagga tattattgct tctttcaagc ttgctgggat atagagtgat tttgtacgaa
     2401 ttttatgtat tttttaatac agatgcatgt ataaatgata cttcttcaaa actttttgag
     2461 caaccaaaaa tgtgatatcc tgcttcgttc agttttttta ctatctctac tgacttatat
     2521 gtttgcatat caaatagtct gtcgtgaaac tcaattaaca attgattgat ggagattttt
     2581 gttgttaaaa tatcttctaa aacctcatat tcagaccctt ctatatcaat ctttaatacg
     2641 tcgatatgag tatgatgtaa catttctgtt atttttgcga aagatttcat tggaagtttt
     2701 atgctattgt tggtgtcaaa tacatcggat tgaatagtac ttcccgacac gcttttggta
     2761 ttttttggta aatagaatgc agcaaattct tgatccccaa cggagatgcc aaaatcaaaa
     2821 aaatgaaagt ttgcaggcaa tttttgttcc ttaatccatt ttattgattt aggtgttgga
     2881 tcaaatgcat atatcgatga ttggtgatgg ttgctacatg ccatatcaaa gctaatgtct
     2941 ttaccaatgc caaatgagta aataacggat tggtttgtaa gcaagttggg gttgatgtaa
     3001 aaacctccat aactggttcc ataccatttc tttggacacg ttatggtggg ttttaggtgt
     3061 ttaactaaat ccggattcgt gtttttataa agatatgttt tacgaaagtg agagtgaata
     3121 ttttgtctac tattgttaat gaattctttt atttttttca tgttttctta ctgattatcc
     3181 aaatacatta aattgttttt taacaaaact actgcctgca aaggttgtgc aatagtagag
     3241 tatattgttg aaagaaaatc ctgctagcct gaaatataca cgagctcgtc tcatgtcatt
     3301 cataatataa tagtagcgag ctcgcgaacc atgaatcttt tttcgagcaa tacgtgtttg
     3361 tgttgggtag aggttttcaa attgttcaat aaaataaagc gaataataga agtcttgacg
     3421 cctataggtt gggtttaatg ataggtttgt gccatctact atgcgtgtgt ataacgaaga
     3481 gcatatctca atagaatttt gttgatgaaa cagacgcaat acccaatcgt agtctatctt
     3541 accaaagtgt tcctcaaaca aaatattacg taaagaacga tgaaaaagca gtgaaccaag
     3601 ataggtgttt tgaccttgac tggatcgtgt cagtttgctt aaaaaggtgc tatttttctc
     3661 gaaaaaaata aatggttctt tcgattgtgc cacacgttca attgtttttc cagtagaagt
     3721 gtcgataagt gtatagccag agctaatgat gggagctttt tggatatatg gtaatacgct
     3781 actaattcgg ttagtatgcc atacatcgtc ttgatctaca atgcagatgt aatcaccctt
     3841 agcattagca agaccaatat tccgtccttt attcggaccg cctgagtttg tctcagtaga
     3901 aaaaatgcga ataggatatt cgtttaagat ttcgagcgta ttgtcagtcg aacaatcgtc
     3961 gatagcgatg atatcaagtt caaataaact atttatacct tcttgctgta aaatagctcg
     4021 aagcgtattg ttgatatttt ttgatccgtt gaaagtcgta ataataactg aaattgtggg
     4081 cataatttgt aatatttact gagtaaaagt acaagattaa tttgaattaa taaattaatc
     4141 ttgaataaat tgtttgtaat ttgactatta tgttaggtat atatgtgtat ttatgaaatt
     4201 ttacacaaaa tatatagtag attagcctca tttattttta ctatcttact atctttgctg
     4261 aataatttgt tctaaaatgg tgcatcgaaa aatattatta tttacttgat tcttaatgta
     4321 atatggtaaa atacattgat ttagatgtgg ctatacagaa tagcaattct gcttttttta
     4381 aaaaaatgcc aaagtgggta atttctttac tcaaaaagat tatacaccaa gatgaaatga
     4441 atcggatcct aaataaatat gaggattgct atggtgtaga ttttttgcct aagattattg
     4501 aagaattaca attaactgta cagatagaag gggaagaaaa tttaccagct gacggacgat
     4561 gtttttttgc tgcaaatcat ccctttggtt ttgtggatgg gctaattctt accaatactg
     4621 ttggtaaaaa gtatggtaag ttaaaagcta taggtaacga ttcttttttg ctagcaccgc
     4681 atcttaaacc cattattgct gctgtaaatg tatttgggtc taatccaaaa gcgtatttgt
     4741 tggagttgga gaatttgttt gaatcagact cacccattac ccattttcct gcaggtgtgg
     4801 tgtcgcgtat tcgtaacgga aaaattgagg ataaagattg gcaaaaaagc tttatcaaac
     4861 aatctgttaa gtataatcgc cccattgttc cttttcattt tacaggacgc aattcttatt
     4921 tattttatat catttactta attcgtagtt ttctcggaat taaactaaat attgaattaa
     4981 tgctattgcc atacgagatt ttcaataaaa aaggtaaaac tattcatgtg aaaattggca
     5041 aacccattat gcctgaaacc tttgacaatt ctaaaacatc cgctgagtgg gcacagtatg
     5101 tcaaacagca agtgtattcg ctgtaactta ttctatgata tactctttgc tttggctagt
     5161 gtacgtgttg aaatagccca atgatgtgcc ttgcatgttt gtaactgggt tggctggtgt
     5221 tgagccactc cgagcactat tacgtaagtc gttaagcgaa ctcaagtata cgtaaaactc
     5281 tttggtaata gtttgcattt ctatccttac tacatcctct gtttttagca atcgatcaaa
     5341 gcggaacaat actatttcta cgtcttttcc attgtttaat ctatcgtcat aaatgtattc
     5401 ttgttgtttc tcattattaa taaattccac aaagcgataa taatttactt cttcaacagg
     5461 atcgttgaat agcactttta tttcgtagta ttctgtgtta tctgtattcg tatttcctcc
     5521 aataggacca aaaacactcc ctttttcgat taccatgtct gtaaaaggaa caatagaagg
     5581 catagtagaa gtagctgata ttggttggtt gttaactatc aagtcaagga aatacctttt
     5641 cccctctatt ccttttgtcg taattgattg gtaaacgcca ggttcaattt ctgtcaacac
     5701 ttctgtattg ttattttcat ctgatatact cacctgagca cctgttagca acggaaatgt
     5761 gtttgattca tctaaattta ccgaacggct aagtaaaaca cgtatttcat ttgctgcagc
     5821 catagatgcc tctaccacta cttccgattt gttgttctgc aagtcgagct gtattatttc
     5881 tgtacacgaa gtgataccga acgaaataac caaaagccat attattttct tcatcagaat
     5941 ttaaaattat aggttattga tggcataaat gtaaataggt atgtttttac tgcgttgatt
     6001 ttgtcagggt cattttcatc ttgttcaaag tcaatggtaa aggcattttt tcttccataa
     6061 gcattgtaaa cagaaaaatt gaggctactt tcaaatcgtt tcgacttttt aaaatagcga
     6121 gtagctccaa tatccattcg atggtagtgt ggcatgcgag atgcgtttct gtttgtatag
     6181 agcaaaatag tttctccatc tacttggtac ttgccacttg ggaaagtaac cgcattgccc
     6241 gtattataca cccacgtgct tgatagtgtc cattttttac ttagttcgta aatagctacc
     6301 acagaaacat catgagtagc atcgtgtctg gcagggtacc aattgccgtt gtttatccct
     6361 tcaatttgat tttctgttct tgataaggtg tatccaatcc atccggagag tgcaccgtat
     6421 tgttttttca acattatttc taatccatac gctcttccgt tgcctactaa taattcgttt
     6481 tcgatatatt cgtttgcttg aatgtttgct ccgtttttta aatcaagttg gttttgcatc
     6541 catttgtaat atatttcaga cgatacctga taggtgtcat ctaaaaagtg cacaaaatag
     6601 ccagtgctta cttggtcgct aatttgtggt ttgatatttt tacttgaggg tatccatata
     6661 tctgtaggta cggatgatgt ggagtttgtg atgaggtgca aattttgcgt attgcgtgta
     6721 tagctcattt taaaggagtt agcatcattt aaaatgtatg caatattgat tcgtggctct
     6781 atatatagat atgtttttac aaattgattg taatcataaa agcttgtttg aatagtgcca
     6841 tcgttgagat atgtataaaa atcgcctcct ccaagaaggg agaatacatt tcctcttact
     6901 ccataagata cattccaatg gtaatttggt ttccagttgt ttgttatgta taggctgctt
     6961 tcaaaggcat atttttcttg tagcttgaac ggttgaaata gggtagattc tgtgctcaat
     7021 tgccctggag tgatacgatg atgtgttgct tgaataccaa atgagagtgt gttttgagct
     7081 gtcagaaaat attggtaatc gtgtttaagt tgtacgttat ttatttgcga taataaacta
     7141 aacgagtttt cagctaaatt gatacctacc ttatagccat agtcagaata gataaaggaa
     7201 gtattgctaa ataatcgatt gttaaaaata tgattccaac gtactgtggc ggtaatgttt
     7261 ccccaattca tactaaatct gtcaaagaaa ctgaacacat ctctaccagc atatgccgat
     7321 atgaataggc gattattatc attaagttgg tagtttgctt ttgcgttgat gtcgtaaaag
     7381 tatagttggt tgttgtttaa aagcggatcg ctcgatagtt tgagaaataa atcagcgtac
     7441 gttcgtcggg cagttaatag gaacgagcct ttgttgcgta taattggacc ttctacgctt
     7501 atacgagaag aaatcagtcc gatgccacca cttacatggt agtgctggtt gttgccatct
     7561 ttcattctta cgtccattac agaggctagt ctacctccat attcagcacc agccgtacct
     7621 ttgtacaaag taacatcttt gatagcatct ccattgaatg tggaaaaaaa accgagcaaa
     7681 tggttcgcgt tatataccat cgattcgtct aaaagaatta aattgtgcga attattacca
     7741 cctcgcacat gcatgcctcc acttccttct cccatggttt ttacgcctgg ggttaatgtt
     7801 agtgttttaa gaatatcttg ttcaccaaac agtacgggga ttttctgaat agattgtatt
     7861 tcaagctttt ctacaccaat tttgtctgat aaaatagttt cgtttctttt aattgctgat
     7921 acgactacct catccaattg tgtgctagct ggttggattt gaaaattctt tcgaatattc
     7981 tcttgtacct caatggagta ctctaggctg ctatatccaa tataatttac cacaatagta
     8041 tgagtgccct tagccagcga aagggcataa tagccatatg cgtttgaata tacagcacta
     8101 gaaggattgc tttttggata aattgtagct cctgttaatg tttcacctgt tgctttgtcg
     8161 gaaatagtgc cactgataga aaaaacttgt tgggctacca ctgaaatgtg tgaaagaaat
     8221 aggagtgtta tcacacagat aacccta
//
LOCUS       DAOSMY010000015         8168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_8751_length_8168_cov_3.449032, whole genome shotgun sequence.
ACCESSION   DAOSMY010000015 DAOSMY010000000
VERSION     DAOSMY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8751_length_8168_cov_3.449032"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PLD76_00710"
     CDS             <1..1348
                     /locus_tag="PLD76_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HOU26560.1"
                     /translation="PFKIGMYTGASTGDSLDGVLARANAVSFRTPYQSNKDLRTSIND
                     NNVNYFDMYLSMIAQELRYGFMPRPNVAIIEACDITNDGEIVLTSAVGITPTIVRLAD
                     KIIIELNHYHPKGLKGIHDIYEPLDPPYRKEIPIYKPSDRIGSPTLKIDPKKILCIVE
                     TNKPDEVGGFSPIDDVTKRIGENVANFLANEIKRGTIPPQFLPIQSGVGNIANAVMAS
                     LGENPGIPPFEMYTEVIQDSVISLMKQGSIKFASGCSLTVTTPVLQDIYANLDFFKER
                     LVLRPQEISNSPEVSRRMGLIAINTALEADIFGNINSTHVMGTKMMNGIGGAGDFTRS
                     AYLSIYTTPSTAKGGKISAIVPMVSHVDQSEHSVKIIITEHGVADLRGKSPIQRAHEI
                     IDNCVAPEYKDILNDYLKIHAKDQTPHCLCSCFGMHSEFLKSGNMLNIDWKEYENK"
     gene            1358..1894
                     /locus_tag="PLD76_00715"
     CDS             1358..1894
                     /locus_tag="PLD76_00715"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOU26561.1"
                     /translation="MIPFTKQAIRQLTKLKVLALSSFERIEKEITIFAQLEQLQEFKN
                     AKHILCYWALPDEVNTSMLIRTWEKEKKFYLPVVKNTELVIKPYSSSKELQQGVFAIQ
                     EPRTDSEVSLSDIDLIIVPGVAFDELGHRLGRGKGYYDRLLQHTTTTKIALAYSEQIY
                     ESIPTELHDIKMDIVVSA"
     gene            1907..3550
                     /locus_tag="PLD76_00720"
     CDS             1907..3550
                     /locus_tag="PLD76_00720"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU26562.1"
                     /translation="MAIPTFKYQEPFPLGKDTTEYYLLTDQYVSVSNFEGKEILKVDV
                     EGLTQVANVAMRDVAFMLRPAHQQQVAAILADPEASENDKYVALTMLRNAEISAKGVL
                     PFCQDTGTATVFAKKGQHVMTNGNDEEALSFGIYKTYTEENLRYSQTIPLDMYNEINS
                     GTNLPAQIDIMSTQGDEYKFLFVAKGGGSANKTYLFQETKALLNPATLEKFLVEKMKT
                     LGTSACPPYHIAFVIGGTSADAAVKTVKLASTKYYDELPTKGNEGGQAFRDIELEQKI
                     LAAAQESGFGAQFGGKYFAHDVRIIRLPRHGASCPVAMAVSCSADRNIKAKINKKGIW
                     IEKMEDNPGKYIPESMRKAGEGDAIHIDLNRPMKEILAELSKYPVATRLSLNGTIIVG
                     RDIAHAKLKERIDSGEGLPQYVKDHPIYYAGPAKTPQGMACGSMGPTTAGRMDSYVDL
                     FQSQGGSMIMLAKGNRSQAVTDACQKYGGFYLGSIGGPAAILAQNNIKKIECIEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFKQLKPGCVKALK"
     gene            complement(3615..4748)
                     /locus_tag="PLD76_00725"
     CDS             complement(3615..4748)
                     /locus_tag="PLD76_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOU26563.1"
                     /translation="MSVSIKEVVSRKDLKNFVQFGTDLYHDSPYFCPPLFFDEINTLD
                     KKKNSALEVCEAIYYLAYKNNKIVGRIAGIVNHRANEAWGVKKTRFGWFDFIDDTDVS
                     FSLLKAVENWGKAKGMDSINGPVGFTDFDHEGLLIEGFDQLGTLATIYNYPYYAKHYE
                     AYGLTKDIDSLEWKIYTPKEVPERMARISALVKEKYNLRYVKVKSHKELVKRFGYTFF
                     DLIDEAYRPLYNYSPLTESQKKYYCEIYFPMLNFDFLSLVVNDKDELVALGVSMPDIT
                     KALQKCRGKLFPFGFIHILKGLKAKKIDVIDLMLIAVRPDYQNKGVNSLLFDEQTPYY
                     NKYQVKYSESSPVLETNLKSQANYAYFETEQHKRRRIYIKPIV"
     gene            4951..5913
                     /locus_tag="PLD76_00730"
     CDS             4951..5913
                     /locus_tag="PLD76_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU26564.1"
                     /translation="MIKKVLLTGANSMLGVNTALLLLAKGYEVKALLRNSREELNACN
                     IIHGDITKFEDVKDAAIGCDAIIHIAAATNQEMLHLSDYTKINVVATENVCQASLVTS
                     AKRVIFVSTANTIGNGTKEKPANETYPASKPFAENLYAKSKLLAEEKALASSANVLII
                     NPTFMIGAYDSKPSSGQIITMNYGKRIVFAPKGGKNFVAASDVSNAIVNALNSTMIGE
                     KILTGGYNLSFADFYKTMAQTQGYKISVITLPNSLLYAVGIIGSLCRKFGIKTPVSFT
                     NMRILCTREWYTAEKAIHVLQMPQTSIEKAITDCINWFKTNNML"
     gene            5919..6839
                     /gene="queG"
                     /locus_tag="PLD76_00735"
     CDS             5919..6839
                     /gene="queG"
                     /locus_tag="PLD76_00735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOU26565.1"
                     /translation="MNKQQLTDTIKQQALTLGFDAVGIAPSQLLEQDKEYLIKWLNKG
                     YAASMKYMHTHLEKRVNPASLLENTRSVIVVLYHYLPTTQQASQQYKIAKYAYTPDYH
                     YVIKSKLQQLASLLPTENADIQRWFTDSAPVLERRWAQLAGLGWIGKNGCFIHPQLGS
                     YVFIGEIFTEHELVYDTPYTHDHCGTCTRCLQACPTQALIAPRELDANKCISYHTIES
                     KNEIPSSIAQQLKGYIFGCDICQDICPWNAKIVAKEKKDSPTMPYVYYSDQDWLTLDK
                     KKFKQDFRHSPMQRAGYVKLIKNIRTVVEK"
     gene            7000..>8168
                     /gene="metK"
                     /locus_tag="PLD76_00740"
     CDS             7000..>8168
                     /gene="metK"
                     /locus_tag="PLD76_00740"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU26566.1"
                     /translation="MGYLFSSESVSEGHPDKVADQISDALLDQFLAQDASSKVACETL
                     VTTGQIILAGEVKSEAYVDLQKTARNVVKRIGYTKSEYMFDADSCGVLSAIHEQSPDI
                     NRGVERTNPDDQGAGDQGLMFGYACNETKNLMPIALDLSHALLKELAAIRKEGKVMTY
                     LRPDAKSQVTIEYGDDHKPKRIHTIVVSTQHDDFLTPTDTLTQEEADKQMLETIYNDV
                     KNILLPRVIAKDEQYASLFHNNIVLHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYATRYIAKNLVAAGIADEILVQVAYAIGVAKPMNIYVN
                     TYGSAKVNKSDAEIASIIETIFDLRPAKIEHMLALRNPIYQETAAYGHKGRAPRT"
BASE COUNT         2783 a         1527 c         1514 g         2344 t
ORIGIN      
        1 gcctttcaaa ataggcatgt atactggagc ttctacaggc gattcgttag atggagtatt
       61 agcccgtgct aatgcggttt ctttccgtac tccttaccaa tcgaacaaag atttacgtac
      121 gagtattaac gacaataacg taaattattt tgatatgtat ttgtccatga ttgctcaaga
      181 actacgctac ggatttatgc ctcgccccaa tgttgccatt attgaagctt gtgatataac
      241 taacgatggt gaaattgtgc ttacatcagc tgtgggtatt actccaacca ttgttcgttt
      301 agccgacaaa attattatcg aattaaatca ttatcatcca aaaggattaa aaggaattca
      361 cgacatttat gaacctctag atcccccata tagaaaagaa attcctattt acaaaccaag
      421 cgatcgtatt ggttcgccca cgttaaaaat tgacccaaaa aagattttat gtatcgttga
      481 aacaaacaag cctgacgagg taggtggttt ctctccgatt gacgatgtaa caaaacgtat
      541 tggtgaaaat gttgccaact ttttggcgaa tgagattaaa agaggaacca ttccaccaca
      601 atttttaccc atacaatctg gcgtaggcaa tattgcgaac gcagtaatgg cttcattggg
      661 tgaaaaccca ggaattcctc ctttcgaaat gtacacagaa gttattcaag attctgttat
      721 ctcgttaatg aaacaaggaa gtataaaatt tgctagcggt tgctcgctta ctgtaacaac
      781 acccgtattg caagatattt atgccaatct agatttcttc aaagaaagat tagtattacg
      841 tccacaagaa atatcaaaca gtccagaagt atctcgtcgc atgggactaa ttgccattaa
      901 tacagcatta gaagctgata tttttggaaa tatcaacagt actcacgtaa tgggtacaaa
      961 aatgatgaat ggtattggtg gtgctggtga ttttactcgt agtgcttatt tatcaatata
     1021 tactacacct tctactgcaa aaggtggtaa aattagtgct attgtgccga tggtgtcgca
     1081 tgttgaccaa agcgaacatt ctgtaaaaat aattatcact gaacacgggg tagctgattt
     1141 acgtggaaaa agccctatcc aacgagccca tgaaataatt gacaattgtg tggcaccaga
     1201 atacaaagat attttaaatg attatttgaa aattcatgct aaagaccaaa ctcctcactg
     1261 cttatgctct tgttttggta tgcactcaga atttttgaaa tcaggcaata tgttgaatat
     1321 tgattggaaa gaatacgaaa ataaataact aaatgttatg ataccgttta caaagcaagc
     1381 catcagacaa cttacaaaac tgaaagtgct tgctttgtct tcttttgagc gaattgaaaa
     1441 agaaattact atatttgcac agttagagca attacaagaa tttaaaaatg ctaaacatat
     1501 actgtgctat tgggctttac cagacgaagt aaacacatct atgctaattc gcacttggga
     1561 aaaagaaaaa aaattttatt tgcctgtggt aaaaaacact gaattagtga taaaaccgta
     1621 tagctcaagc aaagaattgc aacaaggagt ttttgctatt caagaaccaa ggacagatag
     1681 tgaggtaagt ttgtcggata tcgacttaat tatcgttccg ggtgtagcat ttgatgaatt
     1741 agggcatcga ttaggaagag gcaaggggta ttacgataga ttgttacaac atacaacaac
     1801 aacgaaaata gcgttggcat atagcgagca aatttacgag agcataccca ctgaattaca
     1861 tgatataaaa atggacattg ttgtgtccgc ataaacgata tatagtatgg caataccaac
     1921 atttaagtac caagaaccat ttccgctagg taaagatact acggaatatt atttacttac
     1981 tgaccagtat gtttcagtca gcaattttga aggaaaagaa attttaaaag ttgatgtaga
     2041 aggcttaacg caagttgcca acgtggctat gcgtgatgtg gcatttatgc tcagacctgc
     2101 tcaccaacaa caagtagcag caattttagc cgaccccgaa gccagcgaaa acgacaagta
     2161 tgtggctctt actatgttgc gtaatgctga aatatcggca aaaggagttt tacctttttg
     2221 tcaagataca ggtactgcaa cagtatttgc aaaaaaaggt caacatgtaa tgaccaatgg
     2281 caatgatgaa gaagctttat cttttggtat ttacaaaaca tataccgaag aaaatttgcg
     2341 ttattcgcaa accattccgt tggatatgta caacgaaata aattcaggaa caaatctacc
     2401 tgctcaaatt gacatcatga gcacacaggg tgatgaatat aaatttttat ttgtagccaa
     2461 aggaggcggt tctgcaaata aaacctattt atttcaagaa accaaagcct tgctaaaccc
     2521 tgcaacattg gaaaaattcc tagttgaaaa aatgaaaacc ttaggcactt ctgcctgtcc
     2581 accctatcat atagcatttg taataggtgg cacttcggct gatgcagcag taaaaacggt
     2641 aaaacttgcc tctaccaaat attacgacga actgcccaca aaaggcaacg aaggcggaca
     2701 agcatttaga gatatagaat tagaacaaaa aatacttgct gcggctcaag aatcgggctt
     2761 tggtgctcaa tttggaggca aatactttgc tcacgatgtg cgtatcattc gtttgcctcg
     2821 ccacggagca tcttgccctg tagcgatggc tgtatcgtgt tcggctgatc gcaatataaa
     2881 ggctaaaatt aacaaaaaag gcatctggat agaaaaaatg gaagacaatc cagggaaata
     2941 tattcccgaa tcaatgcgta aagcgggcga aggtgatgcg atacacattg accttaaccg
     3001 tccgatgaaa gaaatattgg ctgaattatc aaaataccct gtggctacca gattatcact
     3061 caatggcacc attattgtag gtagagatat tgctcatgca aaattaaaag aacgcattga
     3121 tagcggtgaa ggcttgcctc aatacgttaa agatcaccca atttattacg ctggaccagc
     3181 aaaaacgcct caaggcatgg cgtgtggttc gatgggtccc actacagctg gacgtatgga
     3241 ttcgtatgtg gatttgtttc agtcgcaagg aggcagcatg attatgttgg caaaaggaaa
     3301 ccgcagtcaa gcagtaactg acgcttgtca aaaatacggc ggtttttatc ttggttctat
     3361 cggcggacca gcggctattt tagctcaaaa caacatcaaa aaaattgaat gcatcgaata
     3421 tcctgaactt ggaatggaag ctatttggaa aattgaagta gaagattttc ccgcttttat
     3481 cttggttgat gacaaaggaa atgatttttt caaacaactg aaacctggtt gtgtaaaggc
     3541 attgaaataa tagcatttca ttagacaaaa aaatgggaga aatcaacgaa cgattttctc
     3601 ccattttttg tttattaaac tatcggctta atataaatac gacgacgttt gtgttgttcg
     3661 gtttcaaaat aggcgtaatt ggcttgactc tttaagtttg tttccaacac aggcgatgac
     3721 tccgaatatt ttacttgata tttattgtaa taaggagttt gttcatcaaa taacaacgaa
     3781 tttacccctt tgttttgata atctggacgt actgcaatta acatcaaatc aattacatca
     3841 atttttttag cttttaaccc ctttaatata tggataaaac caaatgggaa taatttaccc
     3901 ctgcattttt gtaaggcttt ggttatatca ggcatactca ctcctagtgc aaccaattcg
     3961 tctttatcat tcacgaccaa ggatagaaaa tcaaaattaa gcatagggaa ataaatttcg
     4021 caatagtatt ttttttgact ctccgtgagt ggagaataat tgtataaggg acgatatgcc
     4081 tcatctatta aatcaaaaaa tgtgtatcca aaacgtttta ccaattcttt gtgacttttt
     4141 accttgacat agcgtaaatt gtatttttct tttaccaaag ctgaaatacg tgccatacgt
     4201 tcgggcactt ctttgggcgt ataaattttc cattcaaggc tatcaatgtc tttggttaag
     4261 ccatacgctt cgtaatgctt tgcataatac ggataattat aaatagtggc aagtgttcct
     4321 aattggtcaa atccctcaat aagtaaacct tcgtggtcaa agtcagtaaa tccaacaggt
     4381 ccattgatgc tatccatccc tttggctttt ccccaatttt caacagcttt tagtaaacta
     4441 aacgatacat ctgtatcatc gataaaatcg aaccaaccaa aacgcgtttt tttaacaccc
     4501 caagcctcat tggctctatg attaactata ccagctatgc gaccaacaat tttattattt
     4561 ttataggcta aataataaat agcctcacac acttctaaag cagaattctt ctttttatca
     4621 agggtattga tttcatcaaa aaataaaggt ggacaaaaat atgggctatc gtggtataaa
     4681 tcagttccaa attgaacaaa attcttcaag tctttacgtg aaacaacttc tttaatactt
     4741 acagacatat cgtttaattt taaggtaaat tgagtgcaaa gctacaaaag gattttaatt
     4801 tatagttatt tttgctttct ttttttgagc tacaattgat aacatattat ttttcaatta
     4861 tttatattgt gtgtcatgtt tcaataaatt gcattgaaaa tccaaatatg tttagtattt
     4921 ttacgctgtt ttcaagaata tatcaaacgc atgataaaaa aagtactgct tacaggagca
     4981 aacagtatgc ttggcgtaaa tacagcatta ctgttattag caaaaggcta cgaggtgaaa
     5041 gcattacttc ggaactctcg agaagaactt aacgcctgta acattattca cggagacatt
     5101 acgaagtttg aagatgtgaa agatgctgca ataggttgcg atgccattat tcatattgca
     5161 gcagctacca atcaagaaat gttgcaccta tctgattaca caaaaatcaa tgtcgttgca
     5221 accgaaaatg tctgtcaggc atcactagta acaagtgcca aaagggttat cttcgtaagt
     5281 actgccaata ccattggtaa tggaaccaaa gaaaagccag ccaatgaaac gtacccagca
     5341 agtaaacctt ttgcggaaaa tttatatgcc aaaagtaaac tgttggcaga agaaaaagcc
     5401 ttagcaagta gtgcaaatgt cctcatcatt aatcctactt ttatgattgg tgcgtatgat
     5461 agcaagccaa gttcaggaca aataattacc atgaattatg gtaaacggat tgtatttgcc
     5521 cccaaaggag gtaaaaattt tgtggcagca agcgatgtat caaatgccat tgtaaatgca
     5581 ctcaattcaa ctatgatagg agaaaaaatt cttactggtg gatacaatct ttcttttgcc
     5641 gatttttata aaacaatggc tcaaacacaa ggctacaaaa taagcgttat caccttaccc
     5701 aacagtctgt tatatgctgt aggtatcatc ggttcacttt gtagaaaatt cggaattaaa
     5761 acacccgttt cattcaccaa tatgcgtata ttgtgtacaa gagaatggta cactgcagaa
     5821 aaagccatac acgtactaca aatgccacaa acgtctattg aaaaagcaat taccgattgc
     5881 atcaattggt ttaaaacaaa taatatgttg taatggttat gaacaagcaa caactcactg
     5941 acaccatcaa acaacaggct cttacattgg gctttgatgc ggtgggaatt gctccttctc
     6001 aattattaga gcaagacaaa gagtatctca tcaagtggct aaacaaaggc tatgccgcct
     6061 caatgaagta catgcataca catcttgaaa aaagagtaaa tccagcctca ttgctcgaaa
     6121 atacacgttc ggtgattgta gttttgtatc actatttacc tactacccag caagccagtc
     6181 agcaatacaa aatagcaaaa tatgcttata ctcctgatta tcattatgtt atcaaatcta
     6241 agctacagca attagcatca ctattaccaa cagaaaatgc tgatattcaa cgttggttta
     6301 ccgactcggc tcccgtatta gaaagacgtt gggcacaact agctggactg ggttggattg
     6361 gtaaaaatgg gtgttttatt catcctcaat taggatcgta tgtgtttatt ggagaaatat
     6421 ttaccgaaca cgaattggtg tacgacacac cctacacgca cgaccattgt ggcacatgta
     6481 cacgttgttt acaagcatgt cctacgcaag ccttaatagc tccacgagaa ctagatgcca
     6541 ataaatgtat ttcgtaccac acgatagaaa gtaaaaatga aattccttcc tctatcgccc
     6601 aacaactcaa ggggtatatt ttcgggtgtg atatttgcca agatatatgc ccatggaatg
     6661 ctaaaattgt ggcgaaagaa aaaaaggatt ctcccacaat gccctatgtg tattatagcg
     6721 accaagattg gttaacgctg gataaaaaga agtttaaaca ggatttccga cactctccta
     6781 tgcaaagagc gggatatgtt aaactaataa aaaacatacg aacggtagtt gaaaaataaa
     6841 aaataacttc tacctttgta ccatcaaagt ggatggattt ggatagcttg caaccacaga
     6901 aaaaaatgct aaaacaaaaa acatactttt ctgccttttt cgatatccaa aactcaaatc
     6961 acttttatta ttttataaca aactaataat taaagtatta tggggtattt attttcatca
     7021 gaatcagttt ctgaagggca tccagacaag gttgccgacc aaatttctga cgcattactc
     7081 gatcaatttt tagcacaaga tgcgtcttca aaagtagcat gcgaaacctt agttacaact
     7141 ggacaaatta ttttggctgg tgaagtaaaa tcagaagcgt atgtagattt acaaaaaaca
     7201 gcacgcaatg tggttaaacg tattggctac accaaaagcg aatacatgtt tgatgccgat
     7261 tcgtgtggtg tattaagtgc tattcacgaa caatcgcctg atatcaatag aggcgtagaa
     7321 agaaccaatc ctgacgatca aggtgcagga gaccaaggat taatgtttgg ttatgcctgc
     7381 aacgaaacaa aaaacctgat gccgatagca ttggatttat ctcacgcctt gctcaaagaa
     7441 ttagcagcta tacgcaaaga aggtaaagta atgacatact tgcgtccaga tgctaaatcg
     7501 caagttacta ttgaatacgg tgacgaccat aaaccaaaac gcatacatac cattgttgta
     7561 tctactcaac acgatgattt tttaacacca accgatacac ttacacaaga agaagcggac
     7621 aaacaaatgt tagaaaccat ctataatgat gtgaaaaata ttttacttcc acgcgttatt
     7681 gccaaagacg aacaatatgc ctcattattt cacaacaata ttgtgttgca tgtgaatcca
     7741 acaggtaaat ttgtaattgg cggtccacac ggtgatacag gtttaacagg tagaaaaatt
     7801 atcgtagata catacggtgg caaaggagca catggtggtg gtgctttttc tggaaaggac
     7861 ccctcaaaag ttgaccgctc tgctgcctat gctacacgct acattgccaa aaaccttgtg
     7921 gcagctggca ttgctgacga aattttggta caagttgctt atgccattgg tgtagcaaaa
     7981 cctatgaata tttatgtaaa cacgtatggt tcagccaaag taaacaaatc agatgccgaa
     8041 atagcgagca ttattgaaac aatctttgac ctacgaccag caaaaattga acacatgtta
     8101 gctttacgca accccatata ccaagaaact gcagcttacg gacacaaggg acgcgcacca
     8161 cgcaccat
//
LOCUS       DAOSMY010000016         8153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_8781_length_8153_cov_3.871573, whole genome shotgun sequence.
ACCESSION   DAOSMY010000016 DAOSMY010000000
VERSION     DAOSMY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8781_length_8153_cov_3.871573"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..855
                     /gene="rpsB"
                     /locus_tag="PLD76_00745"
     CDS             16..855
                     /gene="rpsB"
                     /locus_tag="PLD76_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU26567.1"
                     /translation="MSRTNFQDLLDAGAHFGHLKRKWNPAMSPYIFMEHNGIHIIDLH
                     KTVVKIDEAAEVLFKIAKQGKKVLFVATKKQAKDIIADKASSVNMPYITERWAGGMLT
                     NFPTIRKAVKKMSVIDKMATDGTFENLSKREKLQISRQREKLEKNLGSIADLTRLPAA
                     LFVVDVMKEQIAVKEANRLGIPVFGIVDTNSNPNNIDYVIPANDDAAKSIEIILKAVV
                     EAIQEGLQERKNDKLELEASEVPVDKMKKSKAPSKVRTKRGIDESALNANVADKFLKG
                     EKE"
     gene            965..1789
                     /gene="tsf"
                     /locus_tag="PLD76_00750"
     CDS             965..1789
                     /gene="tsf"
                     /locus_tag="PLD76_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU26568.1"
                     /translation="MAVTMAEISKLRTMTGAGMMDCKNALTESNGDFDKAVEIIRKKG
                     QAVAAKRSDREASEGCVLAASKEAFAACVALKCETDFVAKNADFIALTQTILDIVMDK
                     QPTTLEEVLALPMGNGTIASSITDRSGITGEKMELGYYEWVKGASTVSYIHPGNKLAT
                     IAAFNLAGTDQTVSRDVAMQIAAMNPISLNRDSVPANVIATELEIGRDKARQEGKPEA
                     MLDKIAEGRLNKFYQESTLLEQAFIKDGKITVQNYLKQANKDLTVTDFKRVTLNQE"
     gene            complement(1862..3901)
                     /gene="metG"
                     /locus_tag="PLD76_00755"
     CDS             complement(1862..3901)
                     /gene="metG"
                     /locus_tag="PLD76_00755"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU26569.1"
                     /translation="MEKKFKRTTITSALPYANGPVHIGHLAGVYIPADIYARYLRLKG
                     EDVLFVGGSDEHGVPITLKAKKEGVTPQDIVDRYHTIIKQSFEDFGISFDVYSRTTSK
                     THHEVASTFFKTLYDKGEFVEKTSEQYFDQEAQQFLADRYIIGTCPHCKNTQAYGDQC
                     EACGTSLSPMDLIDPKSTISGSTPILKETKHWYLPLDKHEAWLRQWILEDHKEWKSNV
                     YGQCKSWLDMGLQPRAVSRDLDWGIPVPVAGAEGKVLYVWFDAPIGYISNTKELLPDT
                     WELWWKDEQTKLVHFIGKDNIVFHCIVFPAMLKAEGSFILPENVPANEFLNLEGDKIS
                     TSRNWAVWLHEYLIDFPAKQDVLRYTLTANAPETKDNDFTWKDLQARNNSELVAIYGN
                     FVNRTLVLTHKYFEGKVPALGELTDIEKSVLKEFEDVQHRVESLLNTYRFRDAQKEAM
                     NLARIGNKYLAETEPWKLAKTDMERVATILNVSLQISANLSIAFEPFLPFSSQKLRSL
                     LGLQTCDWSALGSINLLSEGQTIQSPELLFEKIEDDAIEAQVQRLLDTKKANEVADYK
                     PQPIKENVAFDDFMKLDIRVGTVLDCQKVPKADKLLQFKIEDGMGTRTIVSGIAQYYQ
                     PADLIGKQVCFIANFEPRKLKGIASEGMILSAEDSDGRLVVISPSDLVKNGVKIS"
     gene            4131..5399
                     /locus_tag="PLD76_00760"
     CDS             4131..5399
                     /locus_tag="PLD76_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU26570.1"
                     /translation="MRQFLRSSFFKHTATLLTANSLSQAIALLAYPLLTRIYAPDDFG
                     LFNLFLSIGGILAIIGTGNYHYAIVLPKEETKALACVKLSFILNLIVVGISSFFLLFS
                     SQIGKLFNSESLGEYLYLLPIFVLLTATWNILNYWFTRHQCYKPIAFYQISNSLGSSI
                     LKYMLGIMEFLRGGLLIGTILAQVIAFAVGFIVAWKKGLSRTLHIQRQEIVETATTYK
                     NFPFYSLPHSLINTLGGSLPIILLAPFFSLAEIGFFGMALTLAFRPINIISSSLHQVF
                     YPITTQKVNDNDSIMPFFKRYFLRTFTLLIPLFGVLFFFLPMLTEWLLGNGWHQSGKY
                     IQLMLPWLALSAAVAPITYLADIFSQQRIALLLEILSFIVRFCALMIGIWQQNFYLAI
                     AYYCVASALIILVQLLWYWHLVKQYEIKRI"
     gene            5402..6565
                     /locus_tag="PLD76_00765"
     CDS             5402..6565
                     /locus_tag="PLD76_00765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU26571.1"
                     /translation="MRVLQLPSWYIPEGGMFCTEQSIALKQAGVEVHILANVALPWRK
                     YKSRIYSFPRKPFFTEENGITLLRYYAKRIPFADMWNMKLWVRNTLRLYDMYFEKFGH
                     PDIIHTHSSMWAGYAAALIKQRYGVPYVITEHRGRFSENSIESDELFPLSYHPFLYQA
                     FENAHSIIIVSEQLRKKVALYAGENVPIYTISNVVNTDFYTEKKEQNPIRPFTFINAN
                     SYHFAKAYDVLLDAFEKVYAQYPETTLLLLGNGFDDAQFVVQLNKLRSKNKIEFKGYQ
                     PPEGVLHYLHKSDSFVLSSRIEAQPVAILEAMSCGLPIVCTEVVPSEIVTPTIGVRCK
                     TDNADALAEAMIYMIEHHASFSSTDIRQFAVSVCSQKAVASQILAVYKQIIHG"
     gene            6558..8105
                     /locus_tag="PLD76_00770"
     CDS             6558..8105
                     /locus_tag="PLD76_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HOU26572.1"
                     /translation="MVKLDKHNPISWTSANGVWVAGYCFDTEGNFYEKESLIALLQQI
                     TENHLESFVRSLNGIFACIIERDSETIIIMDKTRLIPLFYAIENNQVIVSNNPYTLFP
                     TSPVVDTSALNEYLYSSVVLDEKTLIKGIFQVQPSSYIICKNNHLDIHHYYTFAVKKH
                     EIAVAKDYNQQFETVLNDVFHRLLTSCKGKQVVVPLSGGYDSRLIVCMLAKLGYKNVL
                     CYTVGRPNNLELIIAQKVAKTLGYKHVFISNETPSYLDDNTFEAYYRYSGYFTNFFWM
                     YEYVGVKTLTEQNIIEKDAIFIPGHSGDFLAGSQLQKAGITLETTLNEVEKLVLEQKF
                     AYGNGLKNAAIKQKMNDFIKQRKDYLPYSVFDDVDLLIKLPKNINNAARIYGFFGHQT
                     RLPFWDNEMIEFFKTLPIEEKQTQKFYHHYLTSKVFQPMNVAFVDELTYPEWRMKWYR
                     FKRQCKQLLPTHIQQYISNPPDYVCMKEISAPLKHQLPKTATNGMGGYNRVFLEWYWQ
                     QVKKIVD"
BASE COUNT         2653 a         1495 c         1458 g         2547 t
ORIGIN      
        1 acaaaaaaca aacacatgtc aagaacaaat tttcaagatt tattagatgc aggtgcacat
       61 tttggtcacc taaaaagaaa gtggaatcca gcaatgtctc catacatttt tatggaacac
      121 aatggtattc acatcattga tttacacaaa acagttgtaa aaatcgatga agcagccgaa
      181 gttttattca aaattgccaa acaaggtaaa aaagtattgt ttgtagcaac aaaaaaacaa
      241 gcaaaagata tcattgctga caaagcaagt tctgtaaata tgccttatat cacagaacgt
      301 tgggctggtg gtatgcttac caacttccct actatccgta aagctgtgaa aaaaatgtct
      361 gttatagata aaatggctac tgatggtaca tttgaaaatc tttctaaaag agaaaaatta
      421 caaatctctc gtcaaagaga aaaacttgaa aaaaacttag gtagtattgc tgatttaaca
      481 cgtttacctg cagctctatt tgtagttgac gtaatgaaag agcaaattgc agtaaaagaa
      541 gctaatcgtt tgggtattcc tgtttttgga attgtagata caaactctaa ccccaacaac
      601 attgactatg ttattcctgc aaatgatgat gcagctaaat caattgagat tatcttaaaa
      661 gcagtggttg aagcaattca agaaggctta caagaaagaa aaaatgataa gctagaattg
      721 gaagcatctg aagtgcctgt tgataaaatg aaaaaatcta aagctccttc aaaagtaaga
      781 acgaagagag gtattgatga gtcagcactt aatgcaaatg ttgcagataa attcttaaaa
      841 ggagaaaaag agtaatattt tttcttttat atttatgaat aggttacaac tcaaactctt
      901 tctttaaaga agtggtggcg gttgtaactt tttttttgac actaaacaaa taaaacatac
      961 tattatggca gtaacaatgg cagaaatatc caaattacgc acaatgactg gtgcgggaat
     1021 gatggattgc aaaaatgctc ttacagagtc gaacggtgat ttcgacaaag cagtagaaat
     1081 aattagaaaa aaaggacaag cagtagccgc aaaacgtagc gacagagaag cttctgaagg
     1141 ttgtgtactt gcagcatcta aagaagcgtt tgcagcatgc gtagctctaa aatgcgaaac
     1201 agattttgtg gctaaaaatg cagattttat agcattaacg caaacaattc ttgatatcgt
     1261 gatggacaaa caaccaacaa cccttgaaga agtgttggct ttaccaatgg gtaatggtac
     1321 tattgcctca tctattacag atagaagtgg tattacaggc gaaaaaatgg agcttggtta
     1381 ttacgaatgg gtaaaaggtg ctagcactgt atcgtacata cacccaggaa ataaattagc
     1441 tactattgca gcttttaacc ttgcaggaac tgaccaaaca gtatctcgtg atgtggctat
     1501 gcaaattgcg gcaatgaatc caatttcttt aaatagagat tctgtacctg caaatgttat
     1561 tgctactgaa ttagaaattg gtcgtgataa agcacgccaa gaaggtaaac cagaagctat
     1621 gcttgataaa attgcagaag gacgtttaaa taaattctac caagaatcta ctttattgga
     1681 acaagctttt atcaaagatg gtaaaataac tgtacaaaac tatttaaaac aagcaaacaa
     1741 agatttaact gttaccgatt tcaaacgtgt aacattgaat caagagtaag ctcttgtgat
     1801 aataaaaata aaaaatggag actgaaaaat cagcctccat tttttataga gttgtactgt
     1861 actatgaaat ttttacgccg tttttcacta aatcgctagg cgaaattacc accaaacgtc
     1921 catcgctatc ttctgccgat aaaatcattc cttcgctggc aataccttta agttttcgtg
     1981 gttcgaaatt ggcaataaag catacttgtt ttcctattaa atcagcaggt tggtaatact
     2041 gagcaatacc cgacacaatc gtacgtgtac ccattccatc ttcaatttta aattgcaaga
     2101 gcttatctgc tttgggtact ttttggcaat ctaacactgt accgacacgt atatctaatt
     2161 tcataaaatc gtcaaacgcc acgttttctt taattggttg tggtttataa tcagctactt
     2221 cattcgcctt tttagtatct aatagtcgtt gtacctgcgc ttcgatagca tcatcttcaa
     2281 tcttttcaaa caacaactcg ggcgattgta tcgtttgacc ctctgagagt aagttaatag
     2341 aacctaaggc actccaatca catgtttgca aaccaagcaa tgaacgtaat ttttgtgaac
     2401 taaaaggtaa aaatggttcg aatgcaattg acaagttagc acttatttgt aaagaaacat
     2461 tcaagatagt tgctaccctt tccatatccg ttttggcaag tttccaaggt tcggtttcgg
     2521 ctaaatattt gtttccaatg cgtgctaaat tcattgcttc tttttgagca tctctaaatc
     2581 gataagtatt gagcaaggac tcaaccctat gttgcacatc ctcaaactct ttcaaaaccg
     2641 acttctctat atcagtaagt tctcccaagg cgggcacttt tccttcaaaa tatttatgcg
     2701 taagcaccaa tgttcggttt acaaaattgc cataaatagc cactaattcg ctattattgc
     2761 gagcttgtaa atccttccaa gtaaaatcat tgtcttttgt ttcgggagca ttggctgtaa
     2821 gtgtatatcg taatacatct tgtttggctg gaaaatcgat taaatattcg tgtaaccaaa
     2881 cagcccaatt gcgtgaagtt gatattttat ctccctcaag gtttaagaac tcattggctg
     2941 gaacattttc aggtaaaata aatgaccctt cagcttttaa catagctgga aacacgatac
     3001 agtgaaacac aatgttgtct ttcccgataa aatgtactag tttagtttgt tcatctttcc
     3061 accacagttc ccatgtatct ggcaacaatt ctttggtatt agaaatataa ccaataggag
     3121 catcaaacca cacatacaag accttgccct ctgctcctgc aactggtaca ggaattcccc
     3181 aatctaagtc tcgacttacg gcacgtggtt gcaaacccat atcgagccag cttttacact
     3241 gtccatacac attgcttttc cattctttgt ggtcttcgag tatccattga cgcaaccaag
     3301 cttcgtgttt atcgagcggt aaataccaat gttttgtttc tttgagaatt ggcgtagaac
     3361 cagagatggt tgatttagga tcaattaaat ccataggact tagcgatgtg ccacaggctt
     3421 cacactgatc accataagct tgtgtatttt tgcaatgcgg acatgtgcca ataatataac
     3481 ggtcggctaa aaactgctgt gcttcttggt cgaaatactg ttcggaagtt ttttctacaa
     3541 actcaccttt atcatacagc gttttaaaaa atgtagaagc tacttcgtgg tgcgtttttg
     3601 atgtagtgcg agaatagaca tcgaacgaaa taccaaaatc ttcgaatgat tgtttaataa
     3661 tagtatgata cctatccaca atatcctgag gcgttacgcc ctcttttttt gctttgagcg
     3721 taatgggcac accatgctca tcagaaccac caacaaacaa gacatcttcg cccttgagac
     3781 gtaaataacg tgcgtaaata tctgcgggaa tatacacgcc agctaggtgt ccgatatgaa
     3841 caggaccgtt ggcataaggc aatgcagaag taatggtagt acgtttaaat ttcttttcca
     3901 tatctgtgct aagtctgatt atcaagtttg tgtttgagta agagtgcaaa gttacatatt
     3961 tttcgtggtt tctttgccta tgtttcgtaa atatttaagg taatcaaaac caataccatg
     4021 ctacctaatt caaagaaaaa gataatcgaa aagaaatttg gcaagatagg caataaaaat
     4081 gtatctttgt tcatcggtat ggattatttt tcgtgattaa atacaaacgg atgcgtcagt
     4141 ttcttaggtc atcgtttttt aaacacacag caacattgct tactgccaat agcttatcac
     4201 aagccattgc cttattggca tatcctttac tgacacgcat ttatgcccct gacgatttcg
     4261 gattgttcaa tctattttta agtatcggcg gaatattggc aattattgga actgggaact
     4321 accattatgc cattgtgttg ccaaaagaag aaacaaaagc cctagcttgt gtaaaattat
     4381 cctttatact gaaccttatt gtagtaggta taagtagttt cttcctactc ttttcatcac
     4441 agattggcaa attatttaat tctgagtcat taggagaata cctgtattta ctccccatat
     4501 tcgtattatt gaccgcaaca tggaacattc ttaactactg gtttacacga caccaatgct
     4561 acaaacccat agcattttat caaattagta acagcttggg ttcttcgata ctaaaatata
     4621 tgttaggtat catggagttt ttacgtggtg gtttattaat tggcactata cttgcacaag
     4681 taattgcttt tgccgtaggt tttatagtgg catggaaaaa aggtttgtca agaactttgc
     4741 atatacaacg acaagaaata gtagaaacgg caacaacata taaaaatttc ccattctatt
     4801 ctttaccaca cagcttaatc aacaccttag gaggaagttt gccaattatc cttttagctc
     4861 catttttttc attagcagaa ataggttttt ttggcatggc acttacgctt gcatttcgcc
     4921 caatcaatat tatttctagt tcgctacatc aagtattcta tcctattacc acccaaaagg
     4981 taaacgacaa tgactccatt atgccatttt ttaaacgata ctttttacga acatttacct
     5041 tgcttatacc cttatttggt gtgctatttt tctttttacc tatgcttacc gaatggttat
     5101 tgggtaatgg ttggcatcaa agcggaaaat atattcagtt gatgctacct tggttagccc
     5161 tatcagcagc agttgcaccc attacatatt tagcagatat tttttctcaa caaagaattg
     5221 ccttgttgct tgaaattctt tcatttattg tgcgattttg tgcattaatg attggaatat
     5281 ggcaacaaaa tttctatctt gccatcgcat attattgtgt tgcaagtgct ttaattattc
     5341 ttgttcaact actttggtat tggcatctgg taaaacagta cgaaattaaa cgaatttgat
     5401 tatgagagtg ttacagttac catcttggta tatccccgaa ggagggatgt tttgtacaga
     5461 acaaagcatt gctctaaaac aagctggcgt tgaggtacat atcttggcta atgtggcact
     5521 tccttggcga aaatacaaat ctcgcattta ttcgtttccc agaaaacctt tttttaccga
     5581 agaaaatggt attactctac tgcgttatta cgccaaaaga ataccttttg ctgatatgtg
     5641 gaatatgaaa ctatgggtta gaaatacatt gcgactatat gatatgtatt ttgagaaatt
     5701 tggtcatcca gatattattc acacacacag tagtatgtgg gctggatacg ctgctgcact
     5761 tatcaagcaa cgttatggcg ttccgtacgt aattaccgaa catagaggtc gtttttctga
     5821 aaattctatt gaatctgacg agctatttcc gttaagctat catccttttt tgtaccaagc
     5881 gttcgaaaat gcacatagta tcatcattgt atcggaacaa ctgcgtaaga aagtggctct
     5941 ttatgctgga gagaatgttc ctatttatac aatttcaaat gttgtaaata cagatttcta
     6001 taccgaaaag aaagaacaaa atcctatccg accattcacc tttatcaatg ccaattctta
     6061 tcattttgcc aaagcctacg atgtattgct agatgctttt gaaaaagtat atgcacaata
     6121 tcctgaaaca actttactgt tattgggcaa tggatttgat gatgctcaat ttgtagttca
     6181 gctcaacaaa ttacgctcaa aaaataaaat agaatttaag ggataccaac cacctgaagg
     6241 cgtattgcat tacttacata aaagcgattc gtttgtttta tcaagccgaa tagaggcaca
     6301 acctgtggct attttagaag ccatgagctg tggattgcct attgtgtgta cagaggtggt
     6361 gccttccgaa attgttaccc ctactatcgg tgtacgttgc aaaacagaca atgcagatgc
     6421 actagcagaa gcaatgatct acatgattga gcatcatgcc agtttctcat ctaccgatat
     6481 taggcaattt gccgtttctg tgtgtagcca aaaagctgta gcttcacaaa ttttggcggt
     6541 gtataaacaa attattcatg gttaaacttg acaaacataa tcccatctcg tggacaagtg
     6601 ccaatggcgt ttgggtagct ggctattgtt ttgatacaga aggaaatttt tatgaaaaag
     6661 aatcacttat tgcgttactc caacaaatca cagaaaatca tttagaatca ttcgtacgtt
     6721 cgcttaatgg aatatttgcc tgcatcattg aacgagatag cgaaacgatt attatcatgg
     6781 ataaaacacg cttgattcct cttttttatg ccatagaaaa taatcaagta atcgtatcca
     6841 ataatccata tacattgttt cccacatcgc cagttgttga tacctctgca cttaatgaat
     6901 acctgtattc ttcggttgtg ttggatgaaa aaacactgat taaagggata tttcaagtgc
     6961 aaccatccag ttatattatt tgtaagaata accaccttga tatacatcat tattacactt
     7021 ttgcagtaaa aaagcacgag atagctgtag caaaagatta caatcagcaa tttgaaacgg
     7081 tgctgaacga tgtttttcat cgattactta cctcgtgcaa agggaaacaa gtagttgtac
     7141 ccttgagtgg tggctacgat tctcgcttaa ttgtatgtat gttagccaaa cttgggtata
     7201 aaaatgtgct ttgctataca gtaggcagac ccaataatct agagttaata atagcccaaa
     7261 aagtagcaaa aacgctagga tataagcatg tatttatctc gaacgaaaca ccctcgtacc
     7321 tagatgacaa tacatttgag gcttattatc gttactctgg ttattttacc aatttctttt
     7381 ggatgtacga atatgtaggg gtaaaaacgc tgactgagca aaacatcatt gaaaaagatg
     7441 ccatatttat tccaggacat tctggcgatt ttcttgctgg tagtcaatta caaaaagcgg
     7501 gaattacgct ggaaactaca ttgaatgagg ttgaaaagct agtgctagaa caaaaatttg
     7561 cgtatggcaa tggcttgaaa aacgctgcta ttaaacaaaa aatgaacgac tttatcaagc
     7621 aacgcaaaga ttatctaccc tatagcgtgt tcgatgatgt agatttgctt ataaaattac
     7681 ctaaaaatat caataatgca gcacgtattt atggcttttt tggtcatcaa actcgcttgc
     7741 ctttttggga caatgaaatg attgaatttt tcaaaacatt acccattgaa gaaaaacaaa
     7801 cacaaaaatt ttaccaccat taccttacaa gcaaggtatt tcagcccatg aatgttgcat
     7861 ttgtagatga attaacatac ccagaatggc gaatgaaatg gtataggttt aaaaggcagt
     7921 gcaaacaatt gttacccact cacattcagc agtatatttc caatccgcct gattatgtgt
     7981 gtatgaaaga aatttctgca ccattaaaac accaactacc aaaaacagca acaaatggga
     8041 tgggtggtta caatcgggta tttttagaat ggtattggca acaggtaaaa aagatagttg
     8101 attaagtagc ctaccactct atctttttca aaaaccattt ttttgcgata tac
//
LOCUS       DAOSMY010000017         8146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_8792_length_8146_cov_4.123223, whole genome shotgun sequence.
ACCESSION   DAOSMY010000017 DAOSMY010000000
VERSION     DAOSMY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8792_length_8146_cov_4.123223"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="tpiA"
                     /locus_tag="PLD76_00775"
     CDS             complement(<1..696)
                     /gene="tpiA"
                     /locus_tag="PLD76_00775"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU26573.1"
                     /translation="MRKNIVAGNWKMNKTLQEGIALATELKDALVGTTPNCDVVIGTP
                     FIHLATVVETVKKSPIKVAAQNCADKESGAYTGEVSASMIKSTGVNYVILGHSERRAY
                     YGETFEILRNKVLLALKNELTPIFCIGELKEEREANKQNEVVKAQLEGSVFNLSATDF
                     GKIVIAYEPVWAIGTGLTATSEQAQEIHAFIRSLIADKYGKEVADSTSILYGGSCNAG
                     NAAELFSKPDVDGG"
     gene            complement(709..1947)
                     /locus_tag="PLD76_00780"
     CDS             complement(709..1947)
                     /locus_tag="PLD76_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOU26574.1"
                     /translation="MKQLVLFICRILFGLVFMFSGFVKAVDPLGSMYKFEDYFIAFGF
                     TSLAPLTLFLAIALAALEFLIGANLVLGVHIKKTSWLALFFMAFMTPFTLYIAIANPV
                     ADCGCFGDAWIIDNWTTFYKNILFSILIITIFIFRNEEKIHLANRTQWMMSGFAILFS
                     VAVSVYGYMRLPIFDFRPYKIGANIIEGMTIPENAPQDEYLTTFIYEKEGKQKEFTLD
                     NFPTGDSTWTFVKQQSVLISKGFRPAIHDFSIDYQGEDITDIVLEDSNYTFLVISSKL
                     EDYSQCYSDKINKLYAYAQQNGYSFYCLTSSFGDAVENFRTLTGANYPIAFSDEVTLK
                     TIIRTNPGVLLVKNATVYNKWHCANLPDFSAPIEDTVFIDVNYPHLWKTVVWLLLLFS
                     IPVALIYVLDKKSTIHNKTN"
     gene            complement(1950..2489)
                     /locus_tag="PLD76_00785"
     CDS             complement(1950..2489)
                     /locus_tag="PLD76_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOU26575.1"
                     /translation="MTTEEQYQHSIATCRDVFSKKLKDYGAAWRIMRPKSITDQLFIK
                     ANRIRSIEIKGTSKIEEDIRAEFIAIVNYGIIGLIQLELGVAEVDDLPAEKALVIYDG
                     YAQKAFDLMMDKNHDYDEAWRSMRISSYTDLILMKIYRTKQIEDNDGKTLISEGVDAN
                     YFDMINYAIFGLIKLHYES"
     gene            complement(2582..3097)
                     /locus_tag="PLD76_00790"
     CDS             complement(2582..3097)
                     /locus_tag="PLD76_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOU26576.1"
                     /translation="MALIKSVRGFNPSIGKDCYLAENATIVGDVSLGEGCSVWFNTVL
                     RGDVNTIKIGNHVNIQDGAVLHTLYQKSTIEIGNYVSVGHNVTIHGASIKDYALIGMG
                     STVLDHVVVGEGAIVAAGALVLSGTIIEPHTIWAGVPAKFVKAVDPEQSKEINQRIAH
                     NYAMYASWYKE"
     gene            complement(3105..4520)
                     /locus_tag="PLD76_00795"
     CDS             complement(3105..4520)
                     /locus_tag="PLD76_00795"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOU26577.1"
                     /translation="MKKYCLIVCIILLPLLVVAQKKEIKAYSIELNLPHLKSEKVYLG
                     RYVMGKPYSNDSIVLDTKGRGVFQGSKPLDEGLFMLYFTDGKYVDFMVGNEQHISVKG
                     DTLHLPANLQFSGASQTKDFFQYMRFLTTLQQDYGRKQEQLKATVDDDTKKQVEKELE
                     ALSQQLLTQQNELIANYPTGMVSVFIKGLRIPDMPEDVENQATSDSLKQVMRYLFYKK
                     HYFDNVNFSDIRTYNTPYIVSNLETYLNRVLVQHPDSIVPAAIDLVEKSKGNDKTFQM
                     MTSFMLNYGIKSTMMGMDKLMVELGNRYYLTGKATWADSTLIESLRKEIKKIETSLVG
                     MKASNMYLANEKGEYSDFYSMFGEQLTVLYFFEPDCGHCKKVTPLLRSFYEKYKNDKR
                     INIVAVYMLLDKKEWSDFIEKNQLQAFTNVWDPNRVSRFWELYDASTTPMMYVMDKNK
                     KILAKKIDVETLEMIVKYELK"
     gene            complement(4564..6345)
                     /locus_tag="PLD76_00800"
     CDS             complement(4564..6345)
                     /locus_tag="PLD76_00800"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOU26578.1"
                     /translation="MNSITTPTSRLKLLQQSMQRCGISVCVIPHADMHQNEYISKSDA
                     YLEFYSGFTGSAGTLVVTLDFIGLWTDGRYAIQAKQEIKNTPIHLFVEGVDGVVDFFT
                     WIEQCIIEGNILGIDASLFSYNHYTKICKLCKKRNVELNLSFQPIDELWINRPKAAFQ
                     LPFILDQHFVGEYVESKLKRVRDKMSLLGATSYLLSALDEIAWLLNCRGSDIAYNPVF
                     KAYMLITHDQCVVFLNRKVCNALLFSYFSSCGVMVYDYEFILSYLQSIRNANVLFDGT
                     MVNAAMIEALDASNKKINVSSPIAQLKAIKNEVEIEGCRRAMRKDGVVWVKLWQWIET
                     ELALSNTLSEDLIAKKITQLKEKQEEFVCESFKSIVAFAQNAAICHYAISSNQLVRKE
                     SLLLVDTGTHYLHGTTDTTRVIGLGELSSMQKKEVTLVLKGHIALAQAVFLKGTKGFQ
                     LDALARQFLWKDGYDYAHGTGHGIGHCLNVHEGYASISPRAVQVALQPGMLITNEPGL
                     YKENEYGMRIENVMLVKEVGNGRSGRFLCFETLTLCPIDMQIIDKTMLTEEELIWLEQ
                     YQQKVFDELSPYLTEEERVWLKNKITN"
     gene            6549..6740
                     /gene="rpsU"
                     /locus_tag="PLD76_00805"
     CDS             6549..6740
                     /gene="rpsU"
                     /locus_tag="PLD76_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU26579.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGVVKELRNRQAFTKPSIE
                     KRQQKIHAVYVQKLHLSEE"
     gene            6801..7685
                     /locus_tag="PLD76_00810"
     CDS             6801..7685
                     /locus_tag="PLD76_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU26580.1"
                     /translation="MTNEFIDYLQYERKYSSHTVLAYKEDLLQFEQFICAQTNMCDFT
                     SIDASLARNWIVSLKQSTYSVASINRKISSLKSFYTYCKKKGIQTTNPFLRIHALKNK
                     KRLPQCIKTTEMEELLSSEDIFTKSAFENSRDKIIIELFYHTGMRRAELINLKDCDFN
                     LFSLTLRVTGKGNKQRLIPFSNSLKESITKYLDMKRKTFENPSDNFIVNNKGGQAYPM
                     LIYRIVQDYIGKVSSISKKSPHIIRHTFASALLNNGAEINAVKELLGHANLAATQVYT
                     HTTFEQLKKIYKQAHPRE"
     gene            7703..8002
                     /gene="raiA"
                     /locus_tag="PLD76_00815"
     CDS             7703..8002
                     /gene="raiA"
                     /locus_tag="PLD76_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU26581.1"
                     /translation="MEVKIQSIKFDASEKLEAYIQKKATKLNKKDDSISLIEVILKVV
                     KPESIENKQADIKVFAPQAELFATKTSNTFEQSVDEALEAIERQLEKYKEKKSVK"
BASE COUNT         2792 a         1626 c         1075 g         2653 t
ORIGIN      
        1 tcctccatct acatctggct tagaaaataa ctctgctgca tttcctgcat tacaactacc
       61 tccataaagg atactagtgc tatcagctac ttctttgcca tatttatctg caattaaact
      121 acgaataaaa gcatgtattt cttgtgcttg ttctgaggtt gcagttaatc ctgttccaat
      181 agcccataca ggctcataag caataactat tttaccaaaa tctgtagcag acaaattaaa
      241 aactgaacct tctaattggg cttttactac ttcattttgt ttatttgctt cacgttcttc
      301 ctttaattcg ccaatacaaa atataggtgt aagttcattt tttaatgcaa gcaatacttt
      361 atttcttaaa atttcaaaag tttcgccata ataagctctt ctttctgaat gtcccaaaat
      421 cacataatta acgcctgtag atttaatcat agaagctgaa acctctccag tataagcccc
      481 tgattcttta tcagcacaat tttgagccgc tacttttata ggtgattttt ttacagtttc
      541 tactactgtt gctaaatgaa taaagggtgt gccaattaca acatcacaat taggtgttgt
      601 tcctaccaaa gcatccttta actccgttgc taatgcaata ccctcttgta gggttttgtt
      661 cattttccag tttcctgcta caatgttttt tctcattttc gtttttgttt aatttgtttt
      721 attatgaatc gttgattttt tatctaacac atatattaga gctacaggta tactaaataa
      781 taaaagtaac cacactaccg tcttccataa gtgaggataa ttcacatcaa taaatacagt
      841 atcctctatc ggggctgaaa aatcgggaag atttgcacaa tgccacttat tatacacggt
      901 tgcatttttc accaacaata cacctggatt tgttcttata atggttttca gtgttacctc
      961 atcagaaaat gctattggat aatttgcccc tgttagcgtt ctaaaatttt ctactgcatc
     1021 gccaaaagat gacgttaggc aataaaacga atatccattt tgttgtgcat aagcatacaa
     1081 cttgtttatc ttatcagaat aacactgact ataatcttct aacttagagc ttatcactaa
     1141 aaaagtataa ttactatctt ccaatacaat atctgtaata tcctctcctt gataatcaat
     1201 tgaaaaatca tgtattgcag gacgaaaacc ttttgatata agtacggatt gttgtttcac
     1261 aaatgtccaa gtagaatctc ctgttggaaa attatctaaa gtgaactcct tttgctttcc
     1321 ttctttttcg tatataaagg tagtgagata ttcatcctgt ggggcattct ctggaatggt
     1381 cattccttca atgatatttg ctccaatttt atacggacga aaatcaaata ttggcaaccg
     1441 catataccca tacacagaaa cagcaacact aaacaatata gcaaaaccac tcatcatcca
     1501 ttgtgttctg tttgccagat gaatcttttc ctcattacga aaaataaaaa tagtgataat
     1561 caaaatagaa aataagatat ttttgtaaaa ggtcgtccaa ttatcaataa tccaagcatc
     1621 gccaaaacat ccacaatcgg ctactggatt agcaatagca atgtataaag taaagggagt
     1681 cataaaagcc atgaaaaaca acgccaacca tgatgttttt ttaatatgta cccctaatac
     1741 taggttagct ccaataagaa actctaatgc agccaacgca atagccaaaa atagtgtcaa
     1801 cggagccaga gaagtaaaac caaaggctat aaaataatct tcaaatttgt acatcgaacc
     1861 aagtggatcc acagccttaa caaagcctga aaacataaac accagtccaa ataaaattct
     1921 acaaataaat aacactaatt gtttcatctc taagattcgt aatgtaattt aattaatcca
     1981 aatatagcat aattaatcat atcaaaatag ttggcatcca ccccttctga tattaacgtt
     2041 ttcccatcgt tatcttcaat ttgttttgtt ctgtatatct tcatcagtat taaatctgta
     2101 tacgaactaa tacgcatact acgccaagct tcgtcataat catgattttt atccatcatc
     2161 aaatcgaaag ctttttgagc atagccatcg tagattacca aggctttttc tgctggtaaa
     2221 tcatctactt ctgcaacacc taactctaac tgtatcaaac caataatacc ataatttact
     2281 atagctataa attcagcacg aatatcttcc tcaatttttg aagtaccctt tatttcaata
     2341 ctccttatac gattggcttt aataaaaagc tgatctgtaa tagattttgg acgcattata
     2401 cgccaagccg caccataatc ttttaatttt tttgagaaaa catccctgca cgttgcaata
     2461 ctatgttgat attgttcttc tgttgtcatg ggttaaatac gttgataata agccacaaag
     2521 ttacactttt tcaatcaaaa caaaaataga atttattatt aaaacaaatc ttcaacattt
     2581 tttactcctt ataccaactt gcatacatag cataattatg agctatgcgt tgattaattt
     2641 cttttgattg ctctgggtca acagctttca caaattttgc aggcacacca gcccaaatag
     2701 tatgtggctc aataatagtg ccacttaata ccaaggcacc agctgctaca atagcccctt
     2761 ctcctactac tacatgatcg agtaccgtac tacccatacc tattagagca tagtctttaa
     2821 tgcttgctcc atgaatcgta acattatgtc caacagaaac gtaattacct atttctatcg
     2881 tagatttttg gtacaatgta tgtaaaactg ccccgtcttg aatatttaca tgattaccta
     2941 tttttattgt attaacatcg ccacgtaata cagtattaaa ccacacgcta caaccttctc
     3001 caagcgacac atcacccaca atcgtggcat tttctgccaa gtaacagtct ttacctatgc
     3061 ttggattaaa tcctctaact gattttatta aagccattgt ttttttattt aagttcgtat
     3121 tttacaatca tttccaaagt ttcaacatca attttttttg ctaaaatctt tttattctta
     3181 tccatcacat acatcatggg tgtagtagaa gcatcataaa gctcccaaaa ccgagaaact
     3241 cggtttggat cccaaacatt cgtaaatgct tgtaattgat ttttttcaat aaaatcagac
     3301 cattctttct tatctaaaag catatatact gctacaatat tgattctttt atcattttta
     3361 tatttctcat aaaaactacg taataatggt gtaaccttct tacaatgacc acaatctggc
     3421 tcaaaaaaat acaataccgt cagctgttct ccgaacatgg aataaaaatc agagtactcc
     3481 cctttttcat tagctaaata catattagaa gccttcatac cgaccaagga cgtttctatt
     3541 tttttaattt cttttcgtaa actttcaatt aaagtagaat ctgcccaagt cgcctttcct
     3601 gttaaataat acctattgcc caattctacc ataagcttgt ccattcccat catagtactc
     3661 ttaataccat aattaagcat aaaacttgtc atcatctgaa acgttttatc attcccttta
     3721 cttttttcaa ctaaatcaat cgctgcagga acaatggagt ctggatgttg cacaagtacc
     3781 ctatttagat aggtttctaa attactaaca atatacggag tattatacgt acgaatatca
     3841 gaaaaattaa cattgtcaaa gtaatgtttt ttgtaaaata aataacgcat cacctgtttc
     3901 agactatcac ttgttgcttg attttcaaca tcttctggca tatctggtat acgtaaacct
     3961 ttaataaaaa cagagaccat accagtggga tagttagcaa tgagctcatt ttgctgtgta
     4021 agtaactgtt gacttaaagc ctccaactct ttttctactt gttttttagt atcgtcatcc
     4081 accgtcgctt ttagttgttc ttgtttacga ccataatcct gttgcaatgt ggttaaaaaa
     4141 cgcatatact gaaaaaaatc ttttgtttgg cttgctccac taaactgaag atttgctggc
     4201 aaatgtaacg tatctccttt caccgaaata tgctgttcat tcccaaccat aaaatcaaca
     4261 tatttgccat ccgtaaaata caacataaac aatccctcat ctaagggctt tgatccttga
     4321 aaaacgccac gcccttttgt gtccaacaca atagaatcgt tggaatatgg cttccccata
     4381 acatatcttc ccaaatacac cttctcactt tttaggtgag gcaaattgag ttcaatagaa
     4441 taagctttaa tctctttctt ttgtgctaca actaacaatg ggagtagaat aatacatact
     4501 atcaaacaat attttttcat gatgctttta atttaatttc tctctataaa attatgtatc
     4561 tcattaattt gttattttat tttttaacca tactcgttct tcttctgtca aatagggcga
     4621 taattcatca aatacttttt gttgatattg ttctagccag ataagttcct cctctgtcaa
     4681 catcgtttta tctataatct gcatatcaat aggacacaac gttagcgtct caaaacacaa
     4741 aaatctacca ctcctaccat tacccacttc cttgactaac attacatttt caatacgcat
     4801 tccatactcg ttttctttat acaacccagg ttcattcgta attaacatac caggctgcaa
     4861 tgccacttgt actgcacgag gactaattga agcataacct tcgtgcacat tcagacaatg
     4921 ccctatacca tgtccagtgc catgtgcata atcgtagcca tctttccaca aaaactgacg
     4981 agctaaagca tctaactgaa acccctttgt cccttttaaa aatacagctt gtgccaatgc
     5041 aatatgacct tttaatacca aggttacttc ttttttttgc atagaagaca attcgcctaa
     5101 gccaataaca cgtgttgtat ctgtagtacc atgaagataa tgtgttcccg tatctaccaa
     5161 tagtaagctc tcttttctaa ctaattgatt actagaaata gcataatgac aaatagcagc
     5221 attttgtgca aacgcaacaa ttgatttaaa actttcacac acaaactcct cttgtttctc
     5281 cttcagttga gttatttttt ttgcaattaa atcctcagat aatgtattgg ataaggctaa
     5341 ttcagtttca atccattgcc ataacttaac ccagactaca ccatccttac gcatagcacg
     5401 cctacaacct tctatttcta cttcattttt gattgctttt aattgtgcaa taggagaaga
     5461 aacattaatc tttttattgg atgcatcaag agcctcaatc atagctgcat tcaccatcgt
     5521 tccatcaaac aatacgttgg catttcgtat tgattgcaaa tacgataaaa taaattcata
     5581 gtcatatacc atcactccac acgacgaaaa atacgaaaac agcaatgcat tgcagacttt
     5641 tctattcaaa aataccacac attgatcatg agttatcaac atatacgcct taaaaacagg
     5701 attatatgca atatcactcc ctctgcaatt cagcaaccaa gctatttcat ccaaagcaga
     5761 caataaataa ctagtagctc ccaataaaga cattttgtct cttactcttt ttaacttgga
     5821 ttctacatat tcaccaacaa agtgctgatc taatataaag ggtaattgaa aagcagcctt
     5881 tggtctattt atccatagct catctatcgg ctgaaaggaa agattaagtt ctacattgcg
     5941 ttttttacat aatttacaaa tttttgtata gtgattatac gaaaacaagg aagcgtctat
     6001 tccaagaata ttcccttcta taatacactg ctcaatccat gtaaaaaaat cgacgacccc
     6061 atccactccc tctacaaaaa gatgaatggg cgtatttttt atttcttgtt ttgcctgaat
     6121 tgcgtaacgt ccatctgtcc ataatcctat aaaatctaaa gttactacca atgtccctgc
     6181 ggaaccagta aaacctgaat aaaattctaa ataagcatcc gacttactta tatattcgtt
     6241 ctgatgcata tcagcatgtg gaatcacaca tactgatata ccacatcgtt gcatgctttg
     6301 ttgcaacaat tttaatctgc tagtcggtgt agttatacta ttcatttata caaatttgac
     6361 caacaaaatt acattaattt attgtaatta catctagctt tttcaatcaa aaaccctata
     6421 aaaacatttt ttgacatttt tttaaaaata gaaaacaaaa aaaagatgaa taaatatttg
     6481 tcaatcaaaa aatgattctg tatttttgtc ccctcaaaat tatacatcat tattaaaaac
     6541 aagaagaaat gattattgta ccagttaaag aaggcgaaaa cattgaaaga gccctaaaaa
     6601 aatttaaaag aaaatttgaa aaaacaggag tagtaaaaga acttagaaac cgtcaagctt
     6661 ttactaaacc ttctattgaa aaaagacaac aaaaaattca cgcagtctac gtacaaaaac
     6721 ttcacttaag cgaagagtaa tttttgtatt tctagcataa atgttgtaca tttgtttctg
     6781 agaaatagac actcatttgt atgacaaacg aattcataga ttatttacag tatgagagga
     6841 aatattcttc tcatactgtt cttgcatata aggaagatct tctgcaattt gaacaattta
     6901 tctgtgccca gaccaacatg tgcgatttta cttccatcga tgcctctttg gcaagaaatt
     6961 ggatagtttc actaaagcaa tctacatata gtgttgctag tatcaataga aaaatatcct
     7021 cacttaaatc tttttatacc tactgcaaaa aaaaaggaat acaaacaacc aatccttttc
     7081 tgcgcattca tgctctaaaa aacaaaaaga gactacctca atgcataaag acaactgaaa
     7141 tggaagaatt actttcaagt gaagatattt ttacaaaatc agcatttgaa aattctcgag
     7201 ataaaattat cattgaatta ttttaccata ctggcatgcg aagagcagaa ttaatcaacc
     7261 taaaagattg tgatttcaat ttattttcct taactttgcg tgtaactgga aagggaaata
     7321 agcagaggct tattcctttt agtaattcac tcaaagaatc aattactaag tatttggata
     7381 tgaagagaaa aacttttgaa aacccttccg ataactttat cgtaaataac aaaggaggac
     7441 aagcataccc aatgttaatt tatcgtattg tgcaagatta tatcggaaaa gttagctcaa
     7501 tatcaaaaaa aagccctcat atcattcgac atacatttgc aagtgcacta ctaaataatg
     7561 gagctgaaat aaatgccgtg aaagagctac tcggtcatgc aaacttagcc gcaacacaag
     7621 tatatacaca tacaacattt gaacaattaa aaaaaatata taaacaagct catccaagag
     7681 aataactaaa aaaattacaa ttatggaagt aaaaattcaa agcattaaat ttgacgcctc
     7741 agaaaaatta gaagcgtata ttcaaaaaaa agccacaaaa ctgaataaaa aagatgattc
     7801 catttcatta atagaagtaa tacttaaagt agtaaaacca gaaagcatcg aaaataaaca
     7861 agccgacatt aaggtctttg ctccacaagc agaattattt gcaaccaaga caagcaatac
     7921 ttttgagcaa tcagttgacg aagccctaga ggcaatagaa cgccaattag aaaaatacaa
     7981 agaaaaaaaa tcagttaaat agtttttttt acaatttgtt ttggtattcc aaaaaaaagc
     8041 tctacatttg caagccgttt tagcaagatt tttgttagaa cggcttttta gccgaaaaat
     8101 gccttcatag ctcagttggc cagagcacgt gatttgtaat ctcggg
//
LOCUS       DAOSMY010000018         8122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_8825_length_8122_cov_4.381678, whole genome shotgun sequence.
ACCESSION   DAOSMY010000018 DAOSMY010000000
VERSION     DAOSMY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8825_length_8122_cov_4.381678"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..629)
                     /locus_tag="PLD76_00820"
     CDS             complement(108..629)
                     /locus_tag="PLD76_00820"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU26582.1"
                     /translation="MTNIAIIVAIDKQNGIGKNNQLLCHLPNDLKHFKQLTSGHTIIM
                     GRKTFESLPNGALPNRKNIVITRNQHVTYKDCEIAYSLDDAISKNAEKNIVFIIGGAS
                     IYEQAISVATVLYITKIDATFDADTFFPHINKNDWTLVKKESHQKDDKNPFDYTFETW
                     VRKTSSSQDILGI"
     gene            complement(642..1436)
                     /locus_tag="PLD76_00825"
     CDS             complement(642..1436)
                     /locus_tag="PLD76_00825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOU26583.1"
                     /translation="MKQYHELLQRVLDEGTQKEDRTGTGTISVFGHQMRFRMEDGFPC
                     LTTKKLHLKSIIHELLWFLNGDTNVRYLQENGVRIWNEWADEKGDLGHIYGYQWRSWP
                     TYDGGFIDQISEAVHTIKNSPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVANGKLS
                     LQLYQRSADIFLGVPFNIASYALLLQMMAQVTGLQAGDFIHTLGDAHIYTNHLEQVQL
                     QLTREPRQLPTMKINPTITSIFDFQFSDFELVNYNPYPHIPGAVAV"
     gene            complement(1471..3444)
                     /locus_tag="PLD76_00830"
     CDS             complement(1471..3444)
                     /locus_tag="PLD76_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU26584.1"
                     /translation="MQFKFSILFLLILVTLSSCSIQARINKADKKYELGEYYVAGNLY
                     KRIYPSIPSKEKELRASVAFKSGNAFRQINDNKRAETAYRNAIRYKYKEPLVYFYAAE
                     VARKNNKHTDAIKFYETFLESNPSHREALNGLSSSKLVLSDWKKPTRYVVSKEELFNS
                     RFSDFSPSFANSRGDLVFFNSTRKLNKKNKASRITGQNNNNIFSSRKNIQGKWEEPLL
                     VEGDINSEFDEGTCSFSFDGQELFFTRSKIEKGKTLGTGIFVSKRSGGSWTSPQQIIL
                     LQDSSLNIAHPALSPNGDYLFFVSDMSGGHGGKDIWKVERTKDGWGIPKNLGETINTA
                     GDEMFPSFKKNGTLYFSSDGHPGFGGLDIFKAEPSENNEWMISNMMSPVNSEADDFGI
                     TFMEKEEKGFFSSNRADRRFFDNIWSFELPKLEYKIEGKVTDNNGDILGDAVIRLVGS
                     NGTIEKISVRRNGTFSQIIAPNANYILLASARGFLNSSQKVSTENSNKNQTFTLNFQL
                     NSISRPVQMNNIFFKFGSAELTPESAVALNELVNLLKDNPTVTIEIAAHTDMIGTETA
                     NTLLSTQRAQSVVDYVKQKGIDTARLIPKGYGKNKPVEVDKTLAKKYTFLSEGVILDE
                     EFITSLPSQQQEIANQINRRTEFKVLKTTFQAY"
     gene            complement(3562..4266)
                     /locus_tag="PLD76_00835"
     CDS             complement(3562..4266)
                     /locus_tag="PLD76_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU26585.1"
                     /translation="MNEQKVKILLCEDDENLGMLLREYLQAKGYDTDLLPDGDAGYKA
                     FMKNTYDVCVFDVMMPKKDGFTLAQEVRAVNTEVPILFLTAKTLKEDILEGFKIGGDD
                     YITKPFSMEELLFRIEAILRRIKGKKLKEKIVFKIGKFVFDSQKQILSIGDKHTKLTT
                     KESELLGLLCANANDILERNYTLKTIWIDDNYFNARSMDVYITKLRKLLKEDESVQII
                     NIHGKGYKLIVPQGVE"
     gene            complement(4259..5788)
                     /locus_tag="PLD76_00840"
     CDS             complement(4259..5788)
                     /locus_tag="PLD76_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU26586.1"
                     /translation="MNKRSIILLSVFMAITFFGLLFLQIKYLNSLLDMRVQHFNEAAK
                     RSLYQLNKTLEEEETLHYFNEALAQRPSQRSTSFIDQTVGLQSASSITIQENLQGSTT
                     TIQQQISISSSPSTTRTVQQISETLQNRLKENFAQKKILLDDIAMRWLKESTDKPIEK
                     RIDFTHMENQLKKELENNGITTPFKVILIDYTGEEVYRTSNFNVPNMEHYFSQLLFPN
                     DFNPKLNILRVYFPYAKEYLTQPVSFMIFSSVLFTIILFLVFLATIILIFRQKRLGEM
                     KTDFMNNMTHEFKTPISSISLAAQMLKDEGITKTPEVLKSISNIIYEETKRLSFQVEK
                     VLETSILEKEKSIMKFKEANVHEIIENVCNNFSIKISSKGGIINTNLQASEYWAMVDE
                     THFTNVIYNLLDNALKYRNLDQSPILKIKSWNEKDILYISVEDNGIGIKKEHLKKIFE
                     KFHRVPTGNVHNVKGFGLGLAYVKKIIEKHKGSIRAESELHKGTKFIITIPSLKKTQY
                     E"
     gene            complement(6005..7168)
                     /locus_tag="PLD76_00845"
                     /pseudo
     CDS             complement(6005..7168)
                     /locus_tag="PLD76_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
     gene            complement(7196..7606)
                     /locus_tag="PLD76_00850"
     CDS             complement(7196..7606)
                     /locus_tag="PLD76_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOU26587.1"
                     /translation="MLLIILFICLGIVIYLLNKYTRRETPAKIADTLTPIIEDTEVCC
                     GAYEVCEKDSLLASSPNAVYFEDEDLDIFRFRDEKSYNAKEITLFEEVFFTLREDEIA
                     EWLKSLQIRNINLPYAIKEEALLIVSERRYAHTH"
     gene            complement(7681..>8122)
                     /locus_tag="PLD76_00855"
     CDS             complement(7681..>8122)
                     /locus_tag="PLD76_00855"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOU26588.1"
                     /translation="IVYPKYVKKRDQQKYNKLVRDIKKVYPFAKVVGKEVERVNPLLE
                     KLPQKERKKYMREYEKALFKQYEPDLKKLTLTQGKLLIKLIDRECEQSSFDLIREYRG
                     GLTAFFWQGFARILGANLKEDFNDAKSEDAMINRIIILIENGQL"
BASE COUNT         2262 a         1365 c         1397 g         3098 t
ORIGIN      
        1 ttcgcaaagg taacaaatta agtgtatttt tttatagata tatcaacttt ttttgtattt
       61 ttttgttaac tactctattt tgtattaatt gcaagacaat gaacaattta gatgcctaat
      121 atgtcttgtg aactactcgt ttttcgtacc catgtttcaa aggtataatc aaagggattt
      181 ttatcgtctt tttgatgtga ttcttttttt acaagagtcc aatcattttt gttgatgtga
      241 gggaagaatg tatcagcatc gaatgtagca tcaatttttg tgatatacaa cactgtagcc
      301 actgatatgg cttgttcgta gattgatgcc cctccgataa taaaaacgat gtttttttca
      361 gcatttttac taatggcatc atctaatgaa taggctattt cacagtcttt gtaagtaaca
      421 tgttgatttc gtgtgataac aatgttcttt ctattgggta acgctccatt tggcaatgat
      481 tcaaaagttt ttctacccat gatgattgtg tgccctgagg ttagttgctt aaaatgcttt
      541 aaatcgtttg gcaaatggca tagtaattgg ttgtttttgc caattccatt ttgcttgtcg
      601 atagctacta taatagcaat atttgtcata taaaatcgat attataccgc cacagctcca
      661 ggtatatgag gatacggatt ataatttact aattcaaaat cactaaattg aaaatcaaag
      721 atagatgtta tggtagggtt gattttcatg gttggtaatt gtcgaggttc acgtgtaagt
      781 tgcagttgaa cctgttccag atggttggtg taaatgtgtg catctcccaa tgtgtgaata
      841 aaatcacctg cttgtagccc agttacttgt gccatcattt gaagtaatag ggcgtatgat
      901 gctatattaa acggaacacc taaaaaaata tcggcacttc gttggtacaa ttgcaaacta
      961 agtttgccat tcgctacata aaactgaaaa aaagcgtggc atggtggtaa gttcatattg
     1021 ggcaaatcgg ccacattcca ggcacttaca attattcggc gtgaatcagg gctgttttta
     1081 atggtatgta cagcttctga tatttggtcg ataaaaccac catcatacgt gggccacgaa
     1141 cgccattggt agccgtaaat atgccccaaa tcaccctttt cgtcagccca ttcgttccat
     1201 atacgaacgc cattttcttg taaataacgt acatttgtat cgccatttaa aaaccacagt
     1261 aattcgtgta tgatggattt tagatgcagt ttttttgtgg ttaaacacgg aaatccgtct
     1321 tccattctaa aacgcatttg gtgtccaaat acacttattg tacctgtacc agttcggtct
     1381 tctttttgtg taccttcatc tagtactcgt tgaagtaatt cgtggtattg tttcatatta
     1441 ctaattgttc atatccattt gtttcactat tcaataagcc tgaaaggttg tttttagtac
     1501 tttaaactct gtacgtctgt ttatttgatt agcaatttct tgttgctgag aaggtaaaga
     1561 tgtaataaat tcctcatcaa gtataactcc ttctgataaa aaagtatatt ttttggcgag
     1621 tgttttatct acttcaactg gtttattttt tccgtatcct tttgggataa gacgagcagt
     1681 gtctattcct ttttgtttta cataatctac tactgattga gctcgttgtg tggataaaag
     1741 tgtgtttgct gtttctgtac caatcatgtc cgtatgtgct gcaatttcaa tagttaccgt
     1801 tggattatcc tttagtagat taaccaattc gtttaaagct actgcagatt ctggtgtgag
     1861 ttctgctgat ccaaatttaa agaaaatatt attcatttgc acaggtcgac tgattgagtt
     1921 taattgaaaa ttgagtgtaa aggtttggtt tttattcgaa ttttcggtag aaactttttg
     1981 cgagctattt aaaaatcctc gtgcagatgc caataaaata tagttcgcat ttggggcaat
     2041 tatttgtgaa aatgtcccat tgcgacgaac agatattttc tcaatagttc cattcgatcc
     2101 tactagtctt atcacagcat caccaagtat atctccatta ttatctgtta cttttccttc
     2161 tattttgtat tcaagttttg gtagttcaaa gctccatata ttgtcaaaga aacgtctgtc
     2221 agcacggttg ctcgaaaaga accctttttc ttctttttcc atgaatgtaa ttccaaaatc
     2281 atcggcttct gagttcactg ggctcatcat attgcttatc atccattcat tgttttcgga
     2341 tggttcggct ttaaaaatat ctaatccgcc gaaaccagga tgcccatcgg aagaaaaata
     2401 caacgtacca ttttttttaa acgaaggaaa catttcatct ccagctgtgt taattgtttc
     2461 tccaagattt tttggaattc cccatccatc ttttgttctt tccactttcc aaatatcctt
     2521 gcctccatgt ccgcctgaca tatcagaaac aaagaataaa tagtctccat tgggagaaag
     2581 ggctggatga gcaatgttca aactcgagtc ttgtaataag atgatttgtt ggggagatgt
     2641 ccatgaacct ccacttcttt tagaaacgaa aatacccgta cctagtgttt tccctttttc
     2701 aattttacta cgggtaaaaa atagctcttg accatcaaat gaaaatgaac aggtgccttc
     2761 atcaaattct gaattaatat ctccttctac taggagtggc tcttcccatt ttccttgaat
     2821 atttttacgg gaactaaaaa tattattatt gttttgtccc gtaatgcggc ttgctttatt
     2881 ttttttattt aattttctgg tagaattaaa aaaaactaag tctcctctgc tgtttgcaaa
     2941 tgaaggggaa aaatcagaaa aacgagaatt gaaaagttct tctttgctaa caacatatcg
     3001 agtaggtttt ttccaatcag atagtactaa tttggatgaa gaaaggccat ttaatgcttc
     3061 acgatgtgaa gggttcgatt ctaaaaacgt ttcgtaaaat ttgatggcat ctgtatgttt
     3121 gttatttttg cgggcaactt ctgctgcata aaaatagacc aaaggttctt tgtatttgta
     3181 acgtatggcg ttacgatagg ctgtttctgc tcttttatta tcgttaattt gacgaaatgc
     3241 attcccagat ttaaatgcga cacttgctct taattctttt tctttagaag gtatagatgg
     3301 ataaattctt ttgtataaat tacccgctac gtaatactct cctaattcat attttttgtc
     3361 tgctttgttt attcgagcct gtatgctaca cgaactgagt gtaacaagta taagaagaaa
     3421 taaaatacta aacttaaatt gcatatgaat tgtgaatggc ttatgagagt acaaatatac
     3481 aatttctttt ttaaaaaata gatagaaaaa aacctcatca tattttgaat gatgaggttt
     3541 ttttctatct caaggtaaag attattccac tccttgagga acaattaatt tatacccttt
     3601 tccatgaatg ttaattattt gcacactttc gtcttctttc aataatttac gaagttttgt
     3661 aatgtatacg tccatgcttc gagcattgaa ataattatca tctatccaaa ttgtttttaa
     3721 tgtataattt ctttctaata tgtcattagc attggcacat aataacccaa gtagttctga
     3781 ttcttttgtg gttaattttg tatgtttatc tcctattgac agaatttgtt tttgcgaatc
     3841 aaatacaaat ttaccgattt taaatacaat tttttccttt aattttttcc cttttatacg
     3901 acgcagaatt gcttctattc tgaatagtaa ttcttccatg ctaaaaggtt ttgtaatgta
     3961 atcatctcct ccaattttaa agccttcgag gatgtcctct ttgagggttt tagctgttaa
     4021 aaacaaaata ggcacttcgg tatttactgc tcgcacttct tgggctagtg taaatccatc
     4081 ttttttgggc atcattacgt cgaatacaca tacgtcgtag gtgtttttca taaatgcttt
     4141 gtatccagca tctccatctg gaagtaggtc tgtatcatag cctttggctt gtaggtattc
     4201 gcgtaaaagc atacctaaat tttcgtcatc ttcgcataga agaatcttta ctttttgttc
     4261 attcatattg tgtttttttt agtgatggta ttgttatgat aaattttgtt cctttatgta
     4321 gttcactttc tgctcgaata gagcctttgt gtttttcaat aatttttttt acataagcca
     4381 aacccaagcc aaaccctttt acgttgtgta catttcccgt aggaacacga tgaaattttt
     4441 caaatatttt tttcaaatgt tcctttttga ttcctattcc attatcttct acactaatgt
     4501 aaaggatatc tttttcgttc catgatttta ttttaaggat aggactttgg tcaagatttc
     4561 gatattttaa tgcattgtct aggagattat aaattacgtt ggtaaagtgc gtttcatcca
     4621 ccattgccca atattctgat gcttgtaaat ttgtattgat aatgcctcct ttactgctta
     4681 ttttgattga aaaattgttg caaacatttt cgattatttc gtggacgttg gcttctttaa
     4741 atttcatgat agatttctct ttttccaaga tggatgtttc tagaaccttt tctacctgaa
     4801 aacttaatcg ttttgtttct tcgtatatta tattagaaat gctttttaat acctcaggag
     4861 ttttcgtaat tccttcatct ttgagcattt gagccgccaa ggaaatgctt gatataggag
     4921 ttttgaactc atgtgtcatg ttattcatga agtccgtttt catttctccc aaacgtttct
     4981 gtctgaatat taagataata gttgctaaaa atactagaaa taaaataatc gtaaacagta
     5041 cagaactaaa tatcataaaa gaaacgggtt gtgtaagata ttcttttgca tacggaaaat
     5101 ataccctcag aatatttaat ttaggattga aatcgtttgg aaataggagt tgtgagaaat
     5161 aatgttccat atttggcaca ttaaaattgg atgttcggta tacctcttct cctgtatagt
     5221 caatcaaaat aactttgaat ggggtagtga ttccattgtt ttctagttcc ttttttaatt
     5281 gattttccat atgtgtaaaa tcaatacgtt tttcgattgg tttgtcagta gattctttca
     5341 accatcgcat agcaatatca tcaagtaaaa tctttttttg agcaaaattt tcttttaatc
     5401 gattttgaag tgtttctgat atttgttgta ctgttcgtgt tgtagagggc gagcttgaaa
     5461 ttgatatttg ttgctgaatg gtagtggtac ttccttgtag attttcttga atagtaatag
     5521 aacttgcact ttgcaatcct actgtctggt cgataaaact ggtgcttcgt tgagaaggac
     5581 gttgagcgag ggcttcattg aaatagtgta gtgtttcttc ttcttctagt gtcttgttaa
     5641 gttggtataa actgcgtttt gctgcttcgt taaaatgttg cacacgcata tctaacaaag
     5701 agttgaggta ctttatttgt aaaaacaata acccaaaaaa ggtaattgcc ataaagactg
     5761 acaaaaggat tatacttcgc ttattcatag gcttattctt aagtttttac gttaagggca
     5821 ttgggaactt gcggtattgc caacctctgg gttgaaatat gtttattaga gcatttcata
     5881 tcttcttttt aacaaaacaa agatatgatt ttaacatttg aattcaaact ttctttagta
     5941 tcatttaaca tttactatgt gggatagcct gttaaatgat accgtacgta gtggcgtttg
     6001 attattcctg atttattgct gcttttttgt ctttcatttt cccatcaaac agttcgtatt
     6061 tgcggtattc acattcgata gaaccattta ggagtagata tttttgtgat gggcggagtc
     6121 caattttata aaatccttcc atgtttgagc taataatcca ggcttcggat ccagggaaac
     6181 gatgttttag cgtttcgcct atttctgcat atagcttatt gatgctattg cccccaatgc
     6241 gttctccgta aggaggattg gtaacaataa gtgttttttg tgttggagtg gggagttcca
     6301 taaaattggc tttttgcaag ctaatatatt tacttaatcc tgcactcttg caattttctt
     6361 ctgttatttt tagagcttga gctgaaatgt ccgacccgta aattttatgc ttaaaaatgc
     6421 gttcgttgct atcgtcatta tacactctat caaatacttc actgtcaaaa tctttccatt
     6481 tttcaaacgc aaattctttt ctaaagactc ccggcggaat attaagtgct atcatagcag
     6541 cttcaatgag gagtgttcca gatccacaca tggggtcgag aaaatccgat tcgcctttcc
     6601 atcctgtgag cataattaaa cctgctgcta acacttcgtt gagaggtgct tctgtttgag
     6661 cacttcgata gcctcgtttg tgtaatgatt cccccgaact atctagtgat attgtgcaag
     6721 tagtgtgtgc aatgtgaata tttaaatata cgtctgggtt tgtaagctta attgaaggac
     6781 gctcgttaaa tttttcttta aaataatcta caatggcatc tttaacttta tacgtaacaa
     6841 attttgagtg gctaaatgtt tctgagtata caacagcact gatagcaaaa ctttctttcg
     6901 ctgaaaagta tttttcccaa tgtatttttt ttatttgggc gtatatttca tctgcatttt
     6961 ttgcttgaaa tgtgaaaata ggttttagaa tacgaattgc tgtacgacag tgaagatttg
     7021 ccttgtacat caactcgtta tttcctgtaa aggagacacc tcttttttct ttttgtatat
     7081 tgtttgctcc tatttgtgct agctcttttg ctaaaatatc ttcgagaccc tgtaatgttt
     7141 ttgcaattaa cggaaattct ttgttcatga tgtttggtga gctagtataa tattcttagt
     7201 gagtatgtgc gtatcttctt tcagatacaa taaggagagc ttcttctttg atagcataag
     7261 ggagattaat gtttctgatt tgcaagcttt ttagccattc ggcaatttca tcttcacgca
     7321 atgtaaaaaa tacttcctca aatagtgtaa tttcttttgc attatatgat ttctcgtcgc
     7381 gaaatcggaa aatatctagg tcttcatctt cgaaataaac agcattaggg gacgaagcta
     7441 ataaggagtc tttttcacat acttcatatg ctccacaaca tacttcagta tcttctatta
     7501 ttggcgttag cgtatcggct attttcgccg gagtttctct tcgtgtgtat ttgttgagga
     7561 gatagataac tatccctaaa cagataaaaa ggataatgag tagcataggt taaggtttgg
     7621 tatgttcatc taatgttgca aagataatga ttttattttt atgaatcata tttctttgtg
     7681 ttataattgt ccgttttcta tcaatataat tatccgatta atcattgcgt cttcactttt
     7741 tgcatcatta aagtcttctt tcaggtttgc tcctaatata cgagcaaaac cctgccaaaa
     7801 gaatgcggtc aatcctcctc tgtattcgcg aataaggtca aaagaacttt gttcgcattc
     7861 tctatctatc aatttaatca ataatttgcc ttgtgttagg gttagttttt ttaaatcggg
     7921 ttcatactgt ttgaataggg ctttttcgta ttctcgcata tatttttttc gttctttttg
     7981 cggaagtttt tctaataaag gattaacccg ttccacttct ttccctacaa ctttggcaaa
     8041 aggatatacc ttctttatgt ctcgtactaa tttattgtat ttttgttgat ctcgtttttt
     8101 tacatattta ggataaacta tg
//
LOCUS       DAOSMY010000019         7839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9249_length_7839_cov_3.642472, whole genome shotgun sequence.
ACCESSION   DAOSMY010000019 DAOSMY010000000
VERSION     DAOSMY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9249_length_7839_cov_3.642472"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /gene="rplR"
                     /locus_tag="PLD76_00860"
     CDS             <1..343
                     /gene="rplR"
                     /locus_tag="PLD76_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU26589.1"
                     /translation="ARRLKIKASVRGKISGTATKPRMSVFKSNSQIYVQIIDDTAIEG
                     SGKTIVSASSLDIKEKMTKTEKSTKVGEVIAKKAKEANIETVVFDRNGFLYHGRIKEL
                     AEAARKSGLKF"
     gene            349..867
                     /gene="rpsE"
                     /locus_tag="PLD76_00865"
     CDS             349..867
                     /gene="rpsE"
                     /locus_tag="PLD76_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU26590.1"
                     /translation="MAGKNNIVKTTNDIELKDRLVAINRVTKVTKGGRTFTFSAIVVV
                     GNEDGIVGWGLGKANEVTTAIAKGVESAKKNLVKVPVLKGTIPHEQFAKFGGAEVFLK
                     PASTGTGVVAGGAMRAVLESVGVTDVLAKSKGSSNPHNLVKATIAALTELRDAYTVAQ
                     NRGISIEKVFKG"
     gene            882..1058
                     /gene="rpmD"
                     /locus_tag="PLD76_00870"
     CDS             882..1058
                     /gene="rpmD"
                     /locus_tag="PLD76_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU26591.1"
                     /translation="MATIKVKQVKSRIKCPKVQKLTLDALGLKKMNRVVEHEATPQIL
                     GMINKVKHLVVVEE"
     gene            1095..1541
                     /gene="rplO"
                     /locus_tag="PLD76_00875"
     CDS             1095..1541
                     /gene="rplO"
                     /locus_tag="PLD76_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU26592.1"
                     /translation="MNLSNLKPAGGSVKTCKRVGRGSGSGLGGTSTRGHKGAKSRSGY
                     SKKVGFEGGQMPIQRRLPKFGFNNVNRIEYKAINLATLQQLAEEKKLEKIGIAELLEA
                     GFIARKHNVKILGNGVLTAKIEVTAHAFSKSAIEAIEKAGGSVIKM"
     gene            1546..2883
                     /gene="secY"
                     /locus_tag="PLD76_00880"
     CDS             1546..2883
                     /gene="secY"
                     /locus_tag="PLD76_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU26593.1"
                     /translation="MKRFIETIKNILKIEELKSRLLLTFLLVVIYRFGSYVALPGVDV
                     NALSALSQQTEGGLMMLLDMFSGGAFSNASVFALGIMPYISASIVVQLLGMAFPYFQK
                     LQREGESGRKKMNQITRYLTVGILLVQGPAYLGNLKMQMGNVFPEGWYFIVSSVIILA
                     AGSMFVLWLGERITEKGLGNGISFIILVGIIAKFPGAIIGEFSSRLNDASGGLVMFLL
                     EIVFLLVVIAASILLVQGTRKIPVQYAKRVVGNQQYGGVRQYIPLKVNAAGVMPIIFA
                     QAIMFVPASLSGLFATDGNVSAFAQAFMDHTSFWYNLVFALMIIAFTYFYTAVTINPT
                     QMAEDMKRNNGFIPGIKPGKKTAEYLDSVMSRITLPGSFFLAIVAILPAFAGLLGISF
                     SFAQFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKNGKIKGRTGSIAAY"
     gene            2912..3682
                     /gene="map"
                     /locus_tag="PLD76_00885"
     CDS             2912..3682
                     /gene="map"
                     /locus_tag="PLD76_00885"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU26594.1"
                     /translation="MIYLKTHDEIELMRESNLLVSRTLAEVAKNIRVGIKTLELDAIA
                     EAFIRKNGALPAFKGYSGFPNTLCVSVNEQVVHGIPSDYMLQEGDIVSVDCGVCKNGF
                     YGDSAYTFLVGEVDNNVKSLLKVTKEALYKGIEQAIHGKRIGDIGNSVQEYCEKNGYS
                     VVREMVGHGIGKHLHESPEVPNFGKKGQGIVLKEGMVIAIEPMINLGKKEIAFKRDGW
                     TTVTVDGLPSAHFEHTVAIKKGRADILSTFDYIEEVLN"
     gene            3694..3912
                     /gene="infA"
                     /locus_tag="PLD76_00890"
     CDS             3694..3912
                     /gene="infA"
                     /locus_tag="PLD76_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU26595.1"
                     /translation="MAKQAAIEQDGTIIEALSNAMFRVELENGHEITAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRISFRYK"
     gene            3931..4047
                     /gene="ykgO"
                     /locus_tag="PLD76_00895"
     CDS             3931..4047
                     /gene="ykgO"
                     /locus_tag="PLD76_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOU26596.1"
                     /translation="MKVRASLKKRTPECKIVRRKGRLYVINKKNPKYKQRQG"
     gene            4073..4453
                     /gene="rpsM"
                     /locus_tag="PLD76_00900"
     CDS             4073..4453
                     /gene="rpsM"
                     /locus_tag="PLD76_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU26597.1"
                     /translation="MAIRIVGVDLPQNKRGEIGLTYIYGVGRSSANKILEEAGVDRDI
                     KVKDWTDEQASKIRDIISAKFKVEGDLRSEVQLNIKRLMDIGCYRGIRHRIGLPLRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            4492..4881
                     /gene="rpsK"
                     /locus_tag="PLD76_00905"
     CDS             4492..4881
                     /gene="rpsK"
                     /locus_tag="PLD76_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU26598.1"
                     /translation="MAKKTATTKKRVVKVDANGQAHISSSFNNVIVTLTNSDGQVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKVAFDLGLRKVKAYVKGPGNGRESAIRTIHG
                     AGIEVTEIVDVTPLPHNGCRPPKKRRV"
     gene            5003..5608
                     /gene="rpsD"
                     /locus_tag="PLD76_00910"
     CDS             5003..5608
                     /gene="rpsD"
                     /locus_tag="PLD76_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU26599.1"
                     /translation="MARYIGPKSKIARKFGEPIFGPDKVLSRRNFPPGQHGNTRRRKT
                     SEYGIQLKEKQKAKYTYGVLEKQFANVFDKASRTKGITGEVLLQLLESRLDNVVYRLG
                     LSPTRSGARQLVGHKHIVVNGKVVNIPSYQVKAGDVVGVREKSKSLEVISNALSGFNH
                     GKYSWLEWEQSSMSGKFLHMPERVDIPENIKEQLIVELYSK"
     gene            5613..6605
                     /locus_tag="PLD76_00915"
     CDS             5613..6605
                     /locus_tag="PLD76_00915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOU26600.1"
                     /translation="MPILAFQKPEKVIMLEADSFHGKFEFRPLEPGFGITIGNALRRI
                     LLSSLDGFAITSFKIDGVEHEFATIPGVIEDVTNIILALKQVRFKQLVEGVDSEKVII
                     NVEKTEVFKAGDIGKYLTGFEVLNPELVLCTMDAKSSFHIELTINKGRGYVPSEENKL
                     PSQELGVIPIDAIYTPIVNVKYVVENYRVEQKTDYEKLVFEIDTDGSIHPKDALKEAA
                     RILIHHFMLFSDEKITLDISDSDSVEEFDEEALHMRQLLKTKLTDLDLSVRALNCLKA
                     ADVETLAELVSFNRNDLLKFRNFGKKSLVELDAKLLSLNLSFGMDITKYKLDKE"
     gene            6612..7124
                     /gene="rplQ"
                     /locus_tag="PLD76_00920"
     CDS             6612..7124
                     /gene="rplQ"
                     /locus_tag="PLD76_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOU26601.1"
                     /translation="MRHNKKFNHLGRKTAHRDAMLSNMAVSLIKHKRISTTLAKAKEL
                     RKFVEPMITKSKEDTTHSRRNVFAQLQDKNAVTMLFAEVSQKVADRPGGYTRILKTGF
                     RLGDAAQMCIIELVDYNENMLKDKVAKKSSKTRRAGKKTKAVAEKVATVKAPKEEVVT
                     EQETPTETVE"
     gene            7227..>7839
                     /gene="pyrH"
                     /locus_tag="PLD76_00925"
     CDS             7227..>7839
                     /gene="pyrH"
                     /locus_tag="PLD76_00925"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU26602.1"
                     /translation="MVYKRILLKLSGESLMANKPYGIDENRLQDYAFQIQEIVNLGVE
                     VAIVIGGGNIFRGMSGVAKGYDRVKGDQMGMLATVINSLALASALESINVVSHVLTAI
                     RMEPIGEYYSKQKAIDYLKKGEVVIVSAGTGNPFFTTDTASALRAIEMETDVMLKGTR
                     VDGIYTADPEKDVNAVKFDEISYDEIYNRNLKVMDLTATTMCKE"
BASE COUNT         2777 a         1006 c         1678 g         2378 t
ORIGIN      
        1 agcaagaaga ttaaaaatca aagctagtgt aagaggaaaa atttccggca ctgctacaaa
       61 acctcgtatg tctgttttta aaagtaattc acaaatttat gtgcaaatta ttgatgatac
      121 agcaatcgag ggatctggaa aaaccatagt ttctgcttct tctcttgata ttaaagagaa
      181 aatgacaaaa acagaaaaat cgactaaagt gggtgaagtt attgcaaaaa aagctaagga
      241 agcgaatatt gaaacagttg tttttgatag aaatgggttt ttatatcacg gtagaattaa
      301 agaattagca gaagctgctc gtaaaagtgg gcttaaattt taaataatat ggcaggaaaa
      361 aataatatag ttaaaacaac aaacgacatc gaattgaaag atagacttgt agctattaat
      421 cgcgttacaa aagttacaaa aggtggtcgt acgtttacat tttcagctat cgtagttgta
      481 ggaaatgaag atggtatagt tggttgggga ttaggtaaag caaatgaagt aactactgct
      541 attgcaaaag gagtagaatc tgctaaaaag aatttggtaa aagttcctgt tttaaagggg
      601 acaatacctc atgaacaatt tgctaaattt ggtggagctg aggtatttct taaaccagca
      661 tcaacaggta caggggtagt agcaggtggt gctatgcgtg ctgtattaga gagtgttggt
      721 gtaacagacg ttcttgcaaa atcaaaaggc tcatcaaatc ctcacaacct tgtgaaagct
      781 acaattgcag cattaactga gcttagagat gcttatactg ttgcacaaaa tagaggtatt
      841 tcaatagaaa aggtttttaa aggttaataa tcacaaacac tatggcaact ataaaagtaa
      901 agcaagtaaa aagtagaatt aaatgtccaa aagttcaaaa attgacattg gatgctttgg
      961 gattaaaaaa aatgaataga gtggttgagc atgaagctac accgcaaata ttaggaatga
     1021 ttaataaagt taagcacttg gtggttgtgg aggaataata atcctttatc aacaacataa
     1081 aaaactgtat aacaatgaat ttgagtaatt taaaacctgc aggaggttct gttaaaacct
     1141 gtaaaagagt tggacgtggt tcaggttcag gcttgggtgg tacatctact agaggtcata
     1201 agggagcaaa gtctcgttct ggatattcta aaaaagtcgg gtttgaagga ggtcagatgc
     1261 ctattcaacg tcgcttacca aaatttggct ttaacaatgt aaatagaatt gaatataaag
     1321 ctattaattt agcaacgttg caacaattag ctgaagaaaa gaagttagaa aaaataggta
     1381 tagcagagtt gttagaagcg ggctttattg cacgtaaaca caatgttaag attttaggta
     1441 acggtgtttt aacagcaaaa attgaggtaa ctgctcatgc tttctcaaaa tctgcaattg
     1501 aagctattga aaaagctggt ggctctgtaa ttaaaatgta agaacatgaa aaggttcata
     1561 gagacaatta aaaatatttt aaagattgaa gaattaaaat ctagattact actaaccttt
     1621 ttgttagtgg taatctatcg ttttggttca tatgtagctc taccaggtgt tgatgtgaat
     1681 gctttatctg cactaagtca acaaaccgaa ggtggtttga tgatgttgct tgatatgttt
     1741 tcaggtggag ctttttcaaa tgcctctgta tttgcattgg gtatcatgcc ttatatttct
     1801 gcttctatcg tagtgcagtt acttggcatg gctttccctt atttccaaaa attacaaaga
     1861 gagggagaga gtggaagaaa gaaaatgaac caaattacac gttatttaac cgttggtatt
     1921 cttttagtac aaggtcctgc ttacttagga aatcttaaaa tgcaaatggg gaatgtgttt
     1981 cctgagggtt ggtatttcat agtttcttct gttattattt tagctgcagg aagtatgttc
     2041 gttctatggc taggagaacg aataacagaa aaaggtttag gaaatggtat ttcatttatt
     2101 attttagtcg ggattattgc gaagtttcca ggcgctataa taggcgaatt ttcttctcga
     2161 ttaaatgatg cttctggtgg tttagtaatg tttttattag agatagtctt tttattggtt
     2221 gttattgctg caagtatttt acttgtacag ggaactagaa agatacccgt acagtatgca
     2281 aagcgtgtgg ttggaaatca acaatatggg ggtgttcgtc agtatattcc gttaaaagtt
     2341 aatgccgctg gtgtgatgcc aatcattttt gcacaagcaa ttatgtttgt gcctgcatca
     2401 ttgtcaggac tatttgctac agatggaaat gtcagtgctt ttgcacaagc atttatggat
     2461 catacaagtt tttggtataa tttagtattt gctcttatga ttattgcatt tacttatttt
     2521 tatactgctg taacgattaa tcctactcaa atggcagaag acatgaaacg taataatgga
     2581 tttattcctg gtattaaacc aggtaaaaaa acagcagaat atttagattc tgttatgtct
     2641 cgtattactt tgcctgggtc ttttttctta gcaattgttg cgattctgcc tgcttttgct
     2701 ggcttattag ggattagttt ttcttttgca caattttttg gtggaacgtc attactcatt
     2761 ttggtaggcg ttgtattaga tactttacaa cagatagaga gccatttgtt gatgaggcat
     2821 tatgatggtt tgatgaaaaa cggtaaaatt aaaggaagaa caggatcaat agctgcttac
     2881 tagcactggt ttgttgaaac aacgttgttt gatgatatat ttaaaaacac atgatgaaat
     2941 agagctgatg cgagaaagca atctattagt gagtagaacg ctggcagaag tagctaaaaa
     3001 tattcgagta gggataaaaa cacttgagtt ggatgctata gcagaagcat ttatacggaa
     3061 aaatggagct ttgcctgcat ttaaaggata tagtggattt cctaatactt tgtgtgtttc
     3121 tgtaaatgaa caggtagtac atggtattcc ttctgattat atgctacaag aaggagatat
     3181 tgtttctgtg gattgtggag tttgtaagaa tggattttat ggagattcag cgtatacgtt
     3241 tttagttggt gaagttgata acaacgttaa gagtttgtta aaagttacaa aagaagcttt
     3301 gtataaagga atcgaacaag cgatacatgg gaaaagaatt ggggatatag gaaattcggt
     3361 tcaagagtat tgtgaaaaga atggatattc tgtagtgcgt gaaatggttg gtcatggtat
     3421 aggtaagcat ttacatgaat ctccagaagt tccaaatttt gggaaaaaag gtcaaggcat
     3481 tgttctaaaa gaagggatgg taatcgcaat tgaaccaatg attaatttag gaaaaaaaga
     3541 gattgcattt aaacgagatg gttggactac tgtaactgta gatggtttac cctcagcaca
     3601 ttttgagcat actgtagcta ttaaaaaagg gagagctgac atactctcaa cgtttgatta
     3661 tattgaagaa gtattaaatt agtaatttat ttcatggcaa aacaagctgc aatagagcaa
     3721 gacggaacaa taattgaagc attgtcaaat gcgatgtttc gtgtagagtt agaaaatgga
     3781 catgaaataa cagcacatat ttctggtaaa atgcgtatgc attatattaa gattttacct
     3841 ggggataaag tgcgtgtgga aatgtcaccg tatgatttaa caaaaggaag gatttctttt
     3901 aggtacaaat aaatattaaa tataagaata atgaaagtaa gagcatcgtt aaaaaagcgt
     3961 actcctgaat gtaagattgt aaggagaaag ggacgtcttt atgtgattaa taaaaaaaat
     4021 ccaaagtata aacaacgtca aggataatta aaaacaaata taaaagttta ttatggcgat
     4081 aagaatagtc ggagtagatt tgcctcagaa taagagaggt gaaataggtt taacctatat
     4141 ctacggagta ggtagaagta gtgctaataa aattttggaa gaggctggtg ttgacagaga
     4201 cattaaagta aaagactgga cagacgaaca agcttctaaa atcagagaca ttattagtgc
     4261 aaagtttaaa gtggagggcg atttgcgttc tgaagtacag cttaacatca aacgattgat
     4321 ggacattggt tgttacaggg gaattcgtca ccgtattggt ttaccattga gaggccaaag
     4381 tacaaaaaac aatgcacgta cacgtaaggg taagaaaaaa acagttgcca ataagaagaa
     4441 agcaactaaa taattaatct gagtaaataa gcataaatat actaacaaga tatggcaaaa
     4501 aaaacagcta ctaccaaaaa aagagtagta aaagtagatg caaatggcca agcacacatc
     4561 tcatcatcgt ttaataatgt tattgtaacg ttgacaaatt ctgatggtca agtaatatca
     4621 tggtcatctg caggtaagat gggttttaga ggctctaaaa aaaatacccc atatgcagca
     4681 caaatggctg cacaagattg tgcgaaagtt gcatttgatt tgggattaag aaaggtgaaa
     4741 gcttatgtta aaggacctgg aaatggtcgt gagtcggcta taagaacaat tcatggagca
     4801 gggatagagg ttacagaaat tgtagatgta acgccattac ctcataatgg ttgtcgtcct
     4861 cctaaaaaga gaagagtata aaaataatct tgacagattc gttttctttt tgtgatgaat
     4921 tacattattt aaccctctct gtaatcgcgg caacaaaatc aaaacgaaaa gtcttcgttt
     4981 aaattcataa aaataaaaac aaatggctag atacatagga ccaaaatcaa aaatagcacg
     5041 taagttcgga gaacctattt tcggaccaga taaagtattg tcaagaagaa attttcctcc
     5101 aggacaacat ggtaatacga gaagaagaaa aacttctgaa tatggcattc agttaaagga
     5161 aaaacaaaaa gctaaataca cctacggtgt gttggaaaaa caatttgcaa atgtttttga
     5221 taaagcaagc agaaccaaag gtattacagg tgaggttttg ttacagttat tagaatcaag
     5281 attagacaat gtagtgtatc gtttgggttt atcaccaaca cgttctggtg ctcgtcagtt
     5341 ggttggacac aagcatattg ttgttaatgg gaaagtcgta aatataccat cttatcaagt
     5401 aaaagctggt gatgtagtgg gtgttcgcga aaaatctaaa tcgttggaag ttatatcaaa
     5461 tgcattgtca ggttttaatc atggaaaata tagttggtta gaatgggaac agtcttctat
     5521 gtctggtaag tttttgcata tgccagaaag agtagatata cctgaaaaca ttaaagagca
     5581 actaattgta gaactttact caaaataata ttatgcctat attagcgttt caaaaacccg
     5641 aaaaagtaat tatgttagaa gctgattcat tccatggaaa gtttgaattc agacctttag
     5701 agcccggatt tggtattacg attggtaatg ctttaagacg gattctattg tcttcattgg
     5761 atggatttgc tattacatca tttaaaattg atggtgtaga gcatgaattt gcaacaattc
     5821 caggagttat cgaagatgtt acaaacatta ttctggctct taaacaagtt cgttttaaac
     5881 agctggttga gggggttgat agcgaaaaag ttataattaa tgttgaaaag accgaagtgt
     5941 ttaaggctgg tgatataggc aagtatctaa ctggttttga agtgttaaat ccagaactag
     6001 ttttatgcac gatggatgca aaaagtagtt ttcacattga gcttactatt aacaaaggaa
     6061 gaggatatgt accatctgaa gaaaataaat tacccagcca agaacttggt gtaattccta
     6121 ttgatgctat ttatactcct attgtaaatg taaagtatgt agtggaaaat tatcgtgttg
     6181 aacaaaaaac tgattatgag aaactagtat ttgaaataga tacagatggt tcaatacatc
     6241 caaaagatgc tcttaaagaa gctgcaagaa tcttaattca tcattttatg ctattttctg
     6301 atgaaaaaat cactttggat atttctgata gtgatagtgt agaagaattt gatgaagagg
     6361 ctcttcatat gcgtcaattg ttaaaaacta aattaactga tttggattta tctgtaagag
     6421 ctttgaattg tttaaaagca gcagatgttg aaacattagc agaattggtt tcgttcaatc
     6481 gtaatgattt attgaagttt agaaattttg gtaaaaaatc attggttgaa cttgatgcta
     6541 aattgttgag tttaaatctt tcatttggca tggatattac gaagtataaa ttagataaag
     6601 agtaaaacga aatgagacat aataagaaat ttaatcattt aggtagaaaa acggcacata
     6661 gagatgctat gttgtctaac atggcagttt cgctaatcaa acataaacgc attagtacga
     6721 cattggcaaa agcaaaggaa ttgcgtaaat ttgttgaacc catgattact aaatcaaaag
     6781 aagatacaac gcactcaaga aggaatgtgt ttgcccaatt acaagataaa aatgctgtaa
     6841 cgatgttatt tgcagaggtt tctcaaaaag tagcagatag accaggtgga tatactcgta
     6901 tactaaagac aggttttcga ttaggagatg cagctcaaat gtgtattatt gaattggttg
     6961 attacaacga aaatatgttg aaagataaag ttgccaagaa gtcttctaaa acgcgtagag
     7021 caggtaaaaa gacaaaagct gttgcagaaa aagtagcaac tgtaaaagcc ccaaaagaag
     7081 aggttgttac agaacaagaa acgccaacag aaacggtaga atagacttct gaattaggat
     7141 agaataagga aaggaatgag tattatgctt gttccttttt ttagtataaa aaaataatgt
     7201 atctttgttc gtaaaatttt taggcaatgg tgtataaacg tattttatta aaattaagtg
     7261 gcgaatcgct catggcgaat aagccgtatg ggatagatga aaatcgatta caagattatg
     7321 cttttcagat tcaagagatt gttaatctag gagtggaagt tgccattgta attggaggtg
     7381 gaaatatttt tagaggaatg agtggcgttg cgaaaggcta tgatagagta aaaggagacc
     7441 aaatggggat gctggctacc gttattaaca gtttagcgtt agcttctgca ttagaatcaa
     7501 taaatgttgt atctcatgtt cttactgcta ttagaatgga gcctattggg gaatattata
     7561 gcaaacaaaa ggcaatagat tatttaaaaa aaggagaggt ggtaattgtt tctgctggta
     7621 caggaaatcc cttttttact actgatactg cctcggcatt gcgtgcaata gaaatggaga
     7681 cagatgtaat gttaaaagga acaagggtag atggtatata tactgcagat cctgagaaag
     7741 acgtaaatgc tgttaaattt gatgaaattt cgtatgatga gatttataat cgcaacctta
     7801 aagttatgga tttaactgcc actacgatgt gcaaggaga
//
LOCUS       DAOSMY010000020         7806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9307_length_7806_cov_3.273384, whole genome shotgun sequence.
ACCESSION   DAOSMY010000020 DAOSMY010000000
VERSION     DAOSMY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9307_length_7806_cov_3.273384"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLD76_00930"
     CDS             complement(<1..634)
                     /locus_tag="PLD76_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU26603.1"
                     /translation="MTNVLITGAYGQLGSELKKLAPQYTQFTYFFTDVDTLDICNKQA
                     ILQFCIDNSIHFILNCAAYTAVDKAEEEEQHSLCYAINAEAVKNLGEVASQIGANIIH
                     ISTDYVFDGKAYLPYTEDITTNPQSLYGKSKLLGEQWLMKSCAHALIIRTSWLYSTFG
                     NNFVKTMIKLGTERTELKVIFDQIGTPTYAADLANTMLTIVAKSTEKATNY"
     gene            complement(652..1206)
                     /gene="rfbC"
                     /locus_tag="PLD76_00935"
     CDS             complement(652..1206)
                     /gene="rfbC"
                     /locus_tag="PLD76_00935"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOU26604.1"
                     /translation="MEIIETKIPGLYIVQPKVFGDHRGYFCETYNEKTFHSLGITNKF
                     CQDNLSKSSYGVIRGLHFQLHPHSQAKLVSAVVGTVYDVAVDIRVGSPTYGEWVGIEL
                     SEENKTQFLIPQGFAHGFSVLSETAIFSYKCDNLYTPSAEGGILYNDPTLQIDWKVPF
                     EKQLVSEKDTKHPTLKDVKTNFVF"
     gene            complement(1236..2105)
                     /gene="rfbA"
                     /locus_tag="PLD76_00940"
     CDS             complement(1236..2105)
                     /gene="rfbA"
                     /locus_tag="PLD76_00940"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOU26605.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPISVLMLA
                     GIREILIISTPQDIHLYENLLGDGTSLGVKFTYAIQPSPDGLAQAFIIGDEFIGNDSV
                     CMVLGDNIFYGYNFSKQLKEAATLEDGAVVFGYYVNDPERYGVAEFDTNGKVLSLEEK
                     PAIPKSNYAVTGLYFYSNDVIQKAKGLKPSARGELEITDLNKLYLQEERLKVQLLGRG
                     MAWLDTGTHDSLLEASNFIATIENRQGLKVACLEEIAFNNGFINKDQLLKLAEPLKKN
                     YYGQYLLKIASQK"
     gene            complement(2167..2697)
                     /locus_tag="PLD76_00945"
     CDS             complement(2167..2697)
                     /locus_tag="PLD76_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOU26606.1"
                     /translation="MIIARQKRKENIAEYILYMWHIEDIIRAYKLDIEAIHTHIIMHF
                     SLDEATRKEMKEWYESLIEMMNKEQVMHTGHLQINQSSVRLLCDFHVELLENEDEKQY
                     HSLYHQTLPLIEEFRHKNALSDVPSIEVCLQFMYGIWLLKIQKKEISTETNQAVVQIG
                     KLLAFLSKKFKNNEEN"
     gene            complement(2712..3107)
                     /locus_tag="PLD76_00950"
     CDS             complement(2712..3107)
                     /locus_tag="PLD76_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOU26607.1"
                     /translation="MIKEEVRIDKWLWAVRLFKTRTLAAEACKKGKITIDTVSVKPSR
                     TIRVNDVIQIKKPPITYAFKVLAVTENRMGAKLVPSFMENVTSEEQYAILELSKISGF
                     IDRDKGTGRPTKKDRRSLESFLDTDWTEE"
     gene            complement(3146..3709)
                     /gene="pth"
                     /locus_tag="PLD76_00955"
     CDS             complement(3146..3709)
                     /gene="pth"
                     /locus_tag="PLD76_00955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU26608.1"
                     /translation="MKYLIVGLGNIGSEYQDTRHNIGFTLLDAFAKASNVFFTEKRYG
                     AVCECKIKNKTMILLKPSTFMNLSGNAVRYWMKEEKIPIENVLILVDDLALPFGVLRL
                     KSKGSDAGHNGLKNIQEILGTDQYPRLRFGIGNNYPKGQQINYVLGKWTLEENTALPS
                     RIQIAIDIIQSFVLAGIQHTMNLYNNK"
     gene            complement(3716..4291)
                     /locus_tag="PLD76_00960"
     CDS             complement(3716..4291)
                     /locus_tag="PLD76_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOU26609.1"
                     /translation="MKTFELSGVTRTDLGKKPSKAVRNDNAIPCVMYGTGSNTHFTVT
                     ESDVRKLIYTPEIFEVHLTVDGKKSVAVMKDLQFHPVTDKVLHIDFLEVDNKKPIVLS
                     VPVKVEGLAEGVKAGGKLSLELRKLKVKALCKDIPENLVVNVEKLGLGKTLKAGQLSY
                     PNLEILNNKDNVIVAVKLTRAARGLAAKGGK"
     gene            complement(4426..5469)
                     /gene="thiL"
                     /locus_tag="PLD76_00965"
     CDS             complement(4426..5469)
                     /gene="thiL"
                     /locus_tag="PLD76_00965"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOU26610.1"
                     /translation="MQTRTTISELGEFGLIERLASTISLQHPTSIKGIGDDAAVIENN
                     EGNSQTLISTDLLLEGIHFDLTYFPLKHLGYKAAIVNFSDIYAMNGTPQQITVSIGIS
                     NRFCIEDIEELYEGIKLACSTYKVDIIGGDTSASLTGLTISITCIGIANTKTIAYRNT
                     AKEGDLICVSGNLGAAYMGLQLLEREKTLFRQTNNAQPDFTGKEYILQRQLKPEARKD
                     IIELLAKESIIPTSMIDISDGLSSETLHICTQSKVGCRIYEDKIPIDYQTASMANEFN
                     MNLVTAALNGGEDYELLFTVPLSMHEKIATLEGISIIGHTTKESLGCNLITRDGQEFQ
                     LKAQGWNALHKND"
     gene            5642..6535
                     /gene="gldA"
                     /locus_tag="PLD76_00970"
     CDS             5642..6535
                     /gene="gldA"
                     /locus_tag="PLD76_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOU26611.1"
                     /translation="MSLYIDSLSKFYGTQHVLKNISFHVESGEIIGFLGPNGAGKSTA
                     MKIITGFVQPDSGSIFVDGIDVLNDTLHAQKIIGYLPEHNPLYLDMYVAEYLLFIAQL
                     YKLGKESKQRVEDIIEKTGLTREYTKKIGQLSKGYRQRVGLAQAMIHNPKVLILDEPT
                     TGLDPNQLVEIRELIKEVGKDKTVILSTHIMQEVQAVCDRYVIIREGSIVADSKKDKI
                     HDTVTIVVEFANPISKEHLFMIDKITAIHAINARSYVVECNEDIRYLLYQFAVEQGVG
                     LVTLHLQEQNLEELFRKLTRN"
     gene            6561..7583
                     /gene="ruvB"
                     /locus_tag="PLD76_00975"
     CDS             6561..7583
                     /gene="ruvB"
                     /locus_tag="PLD76_00975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU26612.1"
                     /translation="MEDNFDIRKGALTDKEQDVDIALRPLSFNDFSGQKSIVDNLKIF
                     VQAARMRGESLDHVLLHGPPGLGKTTLSTIIANELQVGFKITSGPVLDKPGDLAGLLT
                     SLETNDVLFIDEIHRLSPIVEEYLYSAMEDYRIDIMIDKGPSARSIQLDLNKFTLVGA
                     TTRSGLLTSPLRARFGINCHLEYYDNDVLINIIKRSAAILHTPCTQDAAVEIASRSRG
                     TPRIANALLRRVRDFAQVKGNGKMDREIACYALEALNIDKYGLDEIDNKILTTIIDKF
                     NGGPVGLTTISTALGEDAGTLEEVYEPFLIKEGFLKRTPRGREVTQLAYEHLGRIRKA
                     DIGSLF"
     gene            7612..>7806
                     /locus_tag="PLD76_00980"
     CDS             7612..>7806
                     /locus_tag="PLD76_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOU26613.1"
                     /translation="MANIKSLAKETAIYGVSSILGKFLNWLLVPLYTYVLESSADYGV
                     VTNLYSWTALLLVVLTYGMET"
BASE COUNT         2356 a         1303 c         1393 g         2754 t
ORIGIN      
        1 cgtaattggt agctttttct gttgatttgg ctactatggt taacattgta tttgctaaat
       61 cagcagcata ggtaggggtg ccaatttgat caaaaatcac cttgagttcc gtacgttctg
      121 tacctaactt tatcattgtt tttacaaaat tgtttccaaa agtagaatat agccatgagg
      181 tacgaataat aagtgcatga gcacatgatt tcattagcca ttgttctcct aatagttttg
      241 attttccata caatgattga ggattagtcg ttatatcttc agtgtatggg agatatgctt
      301 tgccatcaaa tacataatca gtagaaatat ggattatatt ggctcctatt tgcgaagcca
      361 cttctcctaa gttttttaca gcttcagcat tgatagcata acacaaagag tgttgctctt
      421 cttcttccgc tttatctaca gcagtgtatg cggcacaatt taaaatgaaa tgaatagaat
      481 tatctatgca aaactgtaaa attgcttgtt tattacatat atcaagcgta tcaacatccg
      541 taaaaaaata ggtaaactgt gtatattggg gtgcaagttt tttaagttcg cttcctagct
      601 gaccataagc tccggttatt aatacgtttg tcatacaatt caattttata attaaaatac
      661 aaaattagtt ttcacatcct tcaatgtagg gtgttttgtg tctttttctg aaacaagttg
      721 tttttcaaaa ggaactttcc agtctatttg taaggttgga tcgttatata aaatacctcc
      781 ttctgctgat ggtgtatata gattgtcaca tttgtaagag aaaatagccg tttcgctaag
      841 aactgaaaat ccatgtgcaa atccttgtgg gataagaaat tgtgttttat tttcttcgct
      901 taattcgata cctacccact cgccatatgt aggcgaacca acacgtatat ctacagctac
      961 atcatatacc gtgcccacta ctgccgaaac tagttttgct tgactgtgtg ggtgtagctg
     1021 aaaatgtaaa ccgcgaataa ctccgtatga tgatttagat aaattgtctt gacaaaattt
     1081 gtttgtgatg cccaaagagt gaaatgtttt ttcgttatat gtttcgcaaa aataacctct
     1141 atgatcgcca aatactttgg gttgaacaat atataaacct gggattttcg tttcaataat
     1201 ttccatgtgt acgtttttgc tagtaaaaaa ggatgttatt tttgagatgc aattttcaat
     1261 aaatactgac cgtaatagtt ctttttaaga ggctctgcca attttaataa ctgatctttg
     1321 ttgataaagc cattattgaa tgcaatttct tccaaacaag caacctttaa gccttgtctg
     1381 ttttcaatag ttgcaataaa attagaagct tctaacaagc tgtcgtgtgt gcctgtatca
     1441 agccatgcca tgccacgtcc aagtagttgt acttttaggc gttcttcttg caagtaaagc
     1501 ttgtttaagt cggtaatttc cagttcgcca cgtgccgatg gttttaatcc ttttgctttt
     1561 tgaatcacat cgttgctata aaaatacaag ccagtaacgg cataattaga ttttggtatc
     1621 gctggttttt cttctaaact taaaactttg ccattggtgt caaactcagc tactccatag
     1681 cgttcggggt catttacata ataaccgaat actacagctc catcttcgag tgttgcagct
     1741 tcttttagtt gttttgaaaa attatatcca taaaatatgt tatctcccaa taccatacat
     1801 actgagtcgt tgccaataaa ttcgtcccca ataataaacg cttgtgctag cccatctggt
     1861 gagggctgaa tagcataggt aaattttaca cctaaactag taccatcacc taataagttt
     1921 tcgtataaat ggatgtcttg aggtgtagaa ataattagaa tttctctaat gcctgcaagc
     1981 attaaaacag agattggata atatatcatc ggtttgtcat aaaccggaat aatttgtttt
     2041 gaaatacttt ttgtaattgg atacaaacgt gttccagagc ctccagcaag tacaatgcct
     2101 ttcatatgga ttttttttat ttattagtgt aaatgaattt accgcaccaa agttacaagt
     2161 attttgttaa ttctcctcat tgtttttaaa tttcttagac aaaaatgcta aaagtttacc
     2221 aatttgtact actgcttggt tggtttcggt agaaatctct tttttctgaa tttttagcaa
     2281 ccaaatgcca tacataaatt gcaagcagac ttcgattgaa gggacatcgc ttagtgcatt
     2341 tttatgtctg aattcttcga ttaatggtag cgtttgatga tataatgaat gatactgttt
     2401 ttcgtcttca ttttcgagca attctacatg aaaatcacaa agcaaacgaa cggaactctg
     2461 gttgatttgc aagtgtcctg tatgcatcac ttgttcctta ttcatcattt ctattagact
     2521 ttcgtaccat tctttcattt cttttcttgt ggcttcatca aggctaaaat gcattatgat
     2581 atgtgtgtgt attgcttcaa tatctaactt gtatgctctg ataatatctt caatatgcca
     2641 catatacaaa atgtattcag caatattttc ttttcttttt tgacgggcta taatcatacg
     2701 ttttcttttt tttactcttc tgtccaatcg gtatctaaga aagattctaa cgaacgtctg
     2761 tctttttttg ttggtcgtcc agttcctttg tctctatcga taaagcctga aatctttgat
     2821 aattctaaaa tagcatattg ttcttcggat gttacatttt ccataaagga gggcactaac
     2881 tttgcaccca ttctgttttc tgttaccgct aataccttaa aagcatacgt gatagggggc
     2941 ttttttattt gtattacatc gtttacacgg atagtacgtg atggtttgac agatacagta
     3001 tcaatggtaa tttttccttt tttacaggct tcagcagcaa gtgtacgggt tttgaataaa
     3061 cgtacagccc ataaccattt gtcaattctt acttcttctt ttatcatatc atcctactta
     3121 ttataagtaa ctttgctaaa agaaactatt tgttgttgta cagattcatg gtatgttgaa
     3181 tacctgccaa tacaaaactt tgtattatgt caattgctat ttgtatgcga gaggggagag
     3241 cagtgttttc ttctaacgtc catttgccaa gcacatagtt gatttgttga cctttggggt
     3301 agttgttgcc aattccaaat cgtaaacgag ggtattgatc ggtacctaaa atttcttgaa
     3361 tattctttaa tccgttatga ccagcatcac ttcctttcga ttttagtcgc aataccccaa
     3421 aaggtaaagc taaatcatct actaagataa gtacattttc tatcggaatt ttttcttctt
     3481 tcatccaata acgaactgca tttccactta aattcataaa agtagagggt ttaagtaaaa
     3541 tcattgtttt gtttttgatt ttacattcac acacagcccc gtaccgtttt tcggtaaaaa
     3601 aaacattgga tgccttagcg aaggcatcca atagtgtaaa tcctatgttg tggcgagtat
     3661 cttggtattc gctaccaatg ttacccaatc ctacaatcaa gtatttcatg tgttattatt
     3721 ttccgccttt tgcagctaat cctcttgcag ctcttgttaa ttttacagct actattacgt
     3781 tgtctttgtt gttcaaaatt tcgaggtttg gatagctcaa ctgtccagct tttaatgttt
     3841 ttcctaaacc taatttttca acatttacaa ccaaattttc tggaatgtct ttgcaaagag
     3901 cttttacttt tagcttgcgt aattccaatg ataatttacc ccctgctttt acaccttctg
     3961 ccaagccttc aacttttaca ggcacagata atacaattgg ttttttgttg tcaacttcta
     4021 aaaagtcgat gtgtaaaact ttgtctgtaa cagggtgaaa ttgtaagtct ttcattacag
     4081 ctacactttt tttcccatct acagttaagt gtacttcaaa aatttcgggt gtgtaaatta
     4141 atttacgaac atcgctttcg gttactgtaa agtgagtgtt gctaccagtt ccgtacatta
     4201 cacaaggtat tgcgttgtcg ttacgtactg ctttacttgg ttttttacct aagtctgttc
     4261 ttgttactcc agataattca aatgttttca tgttaatttt ttgttgtgtt actcaaattc
     4321 aagtccagtt ataatattaa gtgattagtg acttgattcc catcacactt tctttcgaaa
     4381 agcggtgcaa aggtatgtat ttttttttat atacaaaaga gatacttagt catttttatg
     4441 taatgcattc caaccttgag cttttaattg aaattcttgt ccgtctcgtg taattaaatt
     4501 acagcctaac gattcttttg tagtgtgtcc aataatgcta atcccttcta aagttgctat
     4561 tttttcgtgc atggataatg gaacagtaaa caataattcg taatcttctc cgccatttaa
     4621 agcagctgtt accaaattca tattgaattc attagccatt gatgccgttt ggtaatcaat
     4681 tggaattttg tcttcgtata tgcgacatcc aactttactt tgggtacata tatgcaatgt
     4741 ttcagacgat aagccatcag atatgtcaat cattgaagtc ggaataatgg attcttttgc
     4801 aagtaattcg atgatatctt tacgggcttc gggttttaat tgtcgttgta aaatatattc
     4861 ttttccagta aaatcaggct gtgcattatt cgtttgacgg aaaagtgttt tttcacgttc
     4921 taataattgc aatcccatgt aggctgcacc caaattgcct gacacacaaa ttaaatcacc
     4981 ctcttttgct gtattacgat acgcaatagt ttttgtattg gctataccaa tgcaggtaat
     5041 actaatagtg agtccagtaa gagaggctga agtatcccct ccaataatgt ctaccttata
     5101 cgtagagcaa gccagtttaa tcccctcata taattcttca atatcttcaa tacagaaacg
     5161 gttagatata ccaatagaaa cagtaatttg ttgaggagtg ccattcatgg catatatatc
     5221 cgaaaagtta acaatagccg ccttgtatcc caaatgtttt aaaggaaaat aggttaaatc
     5281 gaaatgaata ccttctaata ataaatcggt tgaaataagt gtttgagaat taccctcatt
     5341 attttctatt actgcggcat catctcctat acctttaata gaggttggat gttgtagtga
     5401 aatggtactt gctaatcgct caattaagcc aaattctcct aattctgaaa tagttgttcg
     5461 tgtttgcata catttttttc ttgaattgca aagatataga tattttttta catcccaatg
     5521 tcaaagagct acagctgtta tctattttga aatgataaaa aaaataaatt tgtagttaaa
     5581 tatgatataa actccctttt tatgtttaat tttgctcaaa atttatagaa ccttattctg
     5641 catgagtctg tatattgata gtttatctaa gttttatggt acccagcatg tattgaaaaa
     5701 tatttcattt catgtagaaa gtggtgaaat tattggtttt ctaggtccga atggagcagg
     5761 gaagtcaact gcgatgaaga ttattactgg ctttgtacag ccagatagtg gtagtatatt
     5821 tgttgatgga attgatgtgt tgaatgatac tttacatgca caaaaaataa ttggatattt
     5881 gccagagcat aatccattgt atttggatat gtatgttgcc gaatacttat tgtttattgc
     5941 tcagctttat aaattaggta aagaaagtaa acagagagta gaggatataa tagaaaagac
     6001 agggttaact cgagaatata caaaaaaaat tggacaatta tcaaaagggt atcgtcaacg
     6061 agtgggttta gctcaagcca tgattcataa tcccaaagtg cttattttgg atgagcctac
     6121 tactggtctt gatcctaatc aattagttga aatacgtgaa cttattaaag aggtaggcaa
     6181 agataaaaca gtaatactta gcacgcatat tatgcaagaa gtgcaggctg tgtgtgatag
     6241 atatgttatt atacgagaag gatcaattgt tgccgatagc aaaaaagata agattcatga
     6301 tactgtgacc atcgttgtgg aatttgcaaa tccaatatcc aaagaacatt tattcatgat
     6361 agataagata actgctatac acgcaattaa tgcacggtcg tatgtggttg aatgtaatga
     6421 agatatccgg tatttgttgt atcaatttgc cgtagaacaa ggggtgggct tggtaacctt
     6481 gcacttacag gagcaaaatt tggaagaact gtttagaaaa ttaacgcgaa attagttgat
     6541 atagactaaa ataaacatag atggaagata atttcgatat aagaaaagga gctttaaccg
     6601 ataaagaaca agatgttgat attgccttac gtccattaag ttttaatgat tttagcggac
     6661 aaaaaagcat tgtggacaac ctgaaaattt ttgttcaagc agctcgtatg cgtggagaat
     6721 cgttagacca cgtattattg catggacctc ctggattagg taaaacgact ttatcaacta
     6781 taattgccaa tgagttgcaa gttgggttta aaataacttc aggacctgta ttggataaac
     6841 ctggtgattt agcaggtttg ctaaccagtt tggaaacgaa tgatgtgttg tttatagatg
     6901 aaatacatcg tttgagtccg attgtagaag aatatttata ttcagcaatg gaggattatc
     6961 gtattgatat aatgatagat aaaggtccaa gtgctcgctc tatacaacta gatttaaaca
     7021 aatttacact tgtaggagca actactcgta gtggactgct tactagtccg ttaagagccc
     7081 gatttggcat caattgtcat ttagaatatt acgacaatga tgtgcttatc aatatcataa
     7141 agcgttcggc agctatatta cacaccccat gcactcaaga tgcagctgta gagatagcta
     7201 gcagaagtag aggtacccca cgtattgcta atgccttatt gcgtagagta agagattttg
     7261 ctcaagtaaa agggaatgga aaaatggaca gagaaattgc ttgttatgcc ctcgaagcat
     7321 taaatattga taagtatggt ttagatgaga ttgataacaa aattttgact actattattg
     7381 ataaatttaa tggaggacct gtcggattga ctaccatttc gacagctttg ggcgaagatg
     7441 ctggaactct agaggaggtg tacgagccgt ttctaataaa ggaaggtttt ttgaaacgca
     7501 cacctcgtgg tagagaggta acgcaattgg catatgagca tctaggtcgt attagaaaag
     7561 cagatatagg ttctttattt taggttttta ttcatcagaa taatcgtatt catggcaaat
     7621 ataaaaagtc ttgcaaagga aacagcaata tatggagtaa gtagcatttt aggaaaattc
     7681 cttaattggt tacttgttcc tctttatacc tatgttctgg aaagttctgc cgattatggt
     7741 gtagtaacaa acttgtattc ttggacagct ttgttgttag ttgttttgac atacggtatg
     7801 gaaact
//
LOCUS       DAOSMY010000021         7683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9517_length_7683_cov_3.675800, whole genome shotgun sequence.
ACCESSION   DAOSMY010000021 DAOSMY010000000
VERSION     DAOSMY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9517_length_7683_cov_3.675800"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PLD76_00985"
     CDS             complement(<1..1613)
                     /locus_tag="PLD76_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26614.1"
                     /translation="MKITKKSFLFALLVCLPIISMMGNMKVGNNKYERRDYLGAIEQY
                     KKTAEGNSNEKTVANAKYKIGECYSLLNEPKEAVSYLGEAIAGGVPTLEAYVLYGKNL
                     LKMGEYARAKASFEEFDRLSPKDARIKNLIASCDFALKHSSKNPITPEQDLKGINTSE
                     IEYGIGFYQDGIVYASVEQAKKGLSSRMYFSHGKDNFTSSRLVDMIKIRTGQNVGTFA
                     IDTLDNILYYTRCNNNERDNCYIYTARYKKGKWKEKGALNIGNRHANVAHPALSPDRQ
                     RLYFTANLEGGFGGTDIWYVTRNENGKWSKTPINVGSVVNTAGNEAFPYVVGNTLIFA
                     SDGHIGFGGYDLYSAQIDGKAVHKVQNLMKPINSSADDINLIVSEEKNEALLVSSRNI
                     STKDDIFRFEGIFTSMMVSGHAYNDVTKEVLPNTQVVLSGMGKTHTTETNKDGYYYAF
                     IEAGDLYKMFANAVGFLSDMAMIKAEKTTIGSFPVEQDFYLTPASMSISGRVYDMETN
                     EPFINEDVLLLENGTLAQQTKTDITGRYS"
     gene            complement(1640..3766)
                     /locus_tag="PLD76_00990"
     CDS             complement(1640..3766)
                     /locus_tag="PLD76_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU26615.1"
                     /translation="MKKNVPFFLSSNKHQAVTSRLRQLFLGIALFCIVPLWAAVQINS
                     VNTSHVSCNGLSDGSITIVATGGSGVFQYSLDNFVNSIQSNSTFINLPATNYSVYVRD
                     VNNHADNTWQTVSITQPLPLTFNPTKDFIQSVSCHGASTGKIRIEAEGGNAPYLYIIN
                     SLDGSISYSNTVGVFDNLPATTYFVQVKDANGCITNHADMVVPQAELLEAELMSKTKI
                     VCKGDMTASVRFKIKGRTEACGLLKADTTAYYSAKLYNITTGVDVLEGEFTVTNQLHP
                     VLTKNIQVPVLDEFGNPVINEAGNPVKKTVKDTLYAEGCHEQTKMGVVPAYTVDKKGF
                     DCDDYISIGKLGEYAYRLELYRRNCQIGTQIEFVIERSGDIPTLSLNGVNPICDGSSE
                     VIKATIVATPATTTYKWILNETTIATTKDLTRVFTMDDNQGKLKFEAKNTCGTVVSNE
                     IALSVLPRPTGIVTTEKGYLCQGQSSNVTAFLAGKAPFTYTLTGNTEQTTSKVSETVS
                     MSPASTIDITFESLSDANCVATPADITGTSITVYPAPSIDITVNVPSPMVSGRYVTAT
                     VTPGFVDYSLWINNQEIEALATEENVFKSKNFVYGLSSNIFDTKVEDTNGCIWDFSKT
                     EDITVVLFPNVFTPNNDGVNDVFLEGYNLKVFDRLGTVLYSGTKGWNGIYNGVPVNKG
                     VYLYTVDILDENGDTVIIKNTVVVEK"
     gene            4067..4828
                     /locus_tag="PLD76_00995"
     CDS             4067..4828
                     /locus_tag="PLD76_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOU26616.1"
                     /translation="MKQFLCLSAFFLFSISLFAQQAVFKNRYYPNGNLMYEGYFVEDK
                     PVGTIKRYHENGKLKAIQQFATNDTVKVEYFAGDGSQLAKGIFVKGQKQGIWEYFSKE
                     NRLVMVENYTEGLKQGESLVFSESGMVIDRMQYENNVLHGKRIQYHPYGNILASYFYK
                     NGLLDGPFESYYETGQKDEVGYYVANKKHGEWKTYNDDGSFTSVEYKNGKATNQTELD
                     KKLQETMDKNEQGDPTIKDPADYLDQPDAYLNLFR"
     gene            4844..6094
                     /gene="xseA"
                     /locus_tag="PLD76_01000"
     CDS             4844..6094
                     /gene="xseA"
                     /locus_tag="PLD76_01000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU26617.1"
                     /translation="MPPHAHIRLVELNSFVKKLIADNTQPQWITAEISEVNEHYSGHC
                     YLELIEKDDADEHIIAKAKAVICSFTYRMIKPYFETTTHERLMAGMKVLVKVEVSFHE
                     AYGYSLVIKDIDPQYTLGDMARKRALIIQQLYAEGVMDLNKEIDLPLLVQRIAVISSP
                     GAAGYTDFCNQLHGNAFGFVFYHRLFAAVMQGTETEASIINALEAVYEHKELFDVVVI
                     IRGGGATSDLSWFDNYNIAYHCTQFPLPILSGIGHDKDVSVVDMVAHTSLKTPTAVAQ
                     FLVDCMQQTESNLHTVKEEIINYTEQALESWQQRLQLVALQLPNAVKRKVEQAYYTLT
                     SSIQHLQHRSKQNLTQKAYTLDKHQQYVQLVSPIRIFEKGYTLTLKNGKPIHNQILAK
                     GDVVETISLQKRMKSTIIEIQNGL"
     gene            6653..6850
                     /gene="xseB"
                     /locus_tag="PLD76_01005"
     CDS             6653..6850
                     /gene="xseB"
                     /locus_tag="PLD76_01005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU26618.1"
                     /translation="MKKEKNLTYDEAYAKLEAILHKIESNELGVEELLENIKEATTLI
                     DYCKNRLKTIDQEVAKLIEGK"
     gene            complement(6898..>7683)
                     /locus_tag="PLD76_01010"
     CDS             complement(6898..>7683)
                     /locus_tag="PLD76_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOU26619.1"
                     /translation="DSTVILNLRVIDVAIQAPKTAICMGESLSLGATANPNTEWSATW
                     TTQPSTGLSLSNASAKTTDASGTLSGNYIVTLTASITQSAGVFAVTPHTCTATQNIVV
                     HPNPVITDIFTTGRAATILYDQQSGTAPFTFNIGNTVYQHNELFDLPIGEYRVEIQNA
                     EGCKSFRMFTIVPTPIIPDKFFSPNNDGVNDRWNVVGIEFYPNADIQIFDRFGKQMIR
                     YKGSDLGWDGNYLGNPMPTTDYWYIIYVAEINETLSGHFTLKR"
BASE COUNT         2385 a         1366 c         1442 g         2490 t
ORIGIN      
        1 aaggaataac gtcctgtgat atctgttttt gtttgttgag ccaaggtgcc attttctaac
       61 aatagcacat cttcgttgat aaatggctcg ttggtttcca tatcatatac acgtccgcta
      121 attgacatac tagctggtgt aagataaaaa tcttgttcta cagggaatga gccaatagtg
      181 gtcttttctg cctttatcat tgccatatcc gataaaaatc ctactgcatt agcaaacatt
      241 ttgtataagt cgcctgcttc gataaatgca taataatatc catctttatt cgtttcagtg
      301 gtgtgtgttt tacccattcc tgataataca acttgtgtat ttggtagcac ttcttttgtt
      361 acatcattgt aagcatgccc cgataccatc atgctggtaa aaataccttc gaagcggaat
      421 atatcatctt tggtagaaat attgcgacta cttactaaaa gagcttcatt tttttcttct
      481 gacacaatta agttaatgtc atcggctgat gagttgatgg gtttcatcaa gttttgcact
      541 ttatgtacgg ctttgccatc tatttgtgca ctatacaaat catatccacc aaatccaata
      601 tgtccgtctg atgcaaatat gagcgtattg cctactacat aagggaaagc ctcgttgcca
      661 gctgtattta ctacggatcc aacattgata ggagttttac tccattttcc gttttcgtta
      721 cgcgttacgt accaaatatc tgttccacca aatccccctt ctaaatttgc tgtaaaatac
      781 aagcgttgtc tgtcgggcga aagggcaggg tgagcaacat tcgcatgtct gttgccaata
      841 tttaaagctc ccttttcttt ccatttccct tttttataac gggctgtgta gatgtagcaa
      901 ttgtctctct cgttattgtt acaacgggtg taatataaga tgttatcaag tgtgtcgatg
      961 gcaaatgtac ctacattttg acctgttcgt attttaatca tatccaccaa gcgagatgag
     1021 gtgaaattat ctttaccatg cgaaaaatac atacgtgatg atagcccttt ttttgcttgt
     1081 tcaacagagg catatacgat accatcttga taaaaaccaa ttccgtattc aatttcagag
     1141 gtattgattc cttttaagtc ttgctcggga gtaataggat tttttgagct gtgtttaagg
     1201 gcaaaatcgc acgaagcaat taagttttta atacgagcat ctttgggcga aagtctgtcg
     1261 aattcttcaa aagaagcttt agcacgtgca tattctccca ttttgagtaa gtttttaccg
     1321 tatagcacgt atgcttccaa tgtaggaaca cctccagcaa tggcttctcc tagataagaa
     1381 acagcttctt tcggttcatt taaaagggaa taacattctc caattttata tttagcattg
     1441 gctactgttt tttcgtttga gttgccttca gcagtctttt tgtattgttc aattgcacct
     1501 aagtaatcgc gtctttcgta tttattgttt ccgactttca tattacccat catactgata
     1561 attggcaagc ataccagcaa ggcaaatagg aaactttttt tagttatttt catataatgt
     1621 acttttattt cgtttacact tatttttcaa ctactacagt atttttaata atcacagtat
     1681 ctccattttc gtcgagtata tccactgtat ataagtacac tcctttgttt actggaacac
     1741 cattgtatat cccattccac ccttttgttc cggagtacaa tacggtgcct aatcggtcga
     1801 atacttttag gttatatcct tcaagaaata cgtcgtttac tccatcgttg ttaggcgtaa
     1861 atacatttgg gaaaagaaca actgtaatgt cttctgtttt agaaaaatcc cagatacagc
     1921 cattggtatc ttcaactttg gtgtcaaaaa tatttgatga aagtccatac acgaagtttt
     1981 ttgatttaaa cacattttct tcggttgcta atgcttctat ctcttggtta tttatccaca
     2041 aagagtaatc cacaaaccca ggtgttactg tagctgtaac gtaacgtccg ctaaccatcg
     2101 gtgaaggtac atttaccgta atatctatcg aaggtgcagg atatacggta atgcttgtgc
     2161 ctgtgatatc agcaggtgta gcaacacaat tggcatcgct aagcgactca aatgtaatgt
     2221 caatagtaga ggcaggactc atactcactg tttctgacac cttagaggtg gtttgttctg
     2281 tgttgccagt caaggtgtac gtaaatggag ctttacctgc taaaaaggct gttacgtttg
     2341 atgactgtcc ttgacaaagg tatcctttct cggtagttac aatacctgta ggacgaggta
     2401 aaacagacaa ggcaatttcg ttagaaacaa ccgttccgca ggtatttttt gcttcaaatt
     2461 taagtttccc ttgattatca tccatcgtaa atacgcgggt caaatctttg gtagtagcaa
     2521 tggttgtttc gtttaatatc catttgtaag tagtagtggc aggagttgct actatcgttg
     2581 cttttattac ttcagaagaa ccatcgcata tcggattcac tccatttaat gataaggtag
     2641 ggatatcacc actccgttcg ataacaaatt caatttgtgt acctatttga caatttcttc
     2701 tatataactc caaacggtac gcatattctc ctagtttccc gatagaaata taatcatcgc
     2761 aatcaaatcc ttttttatct acggtataag cgggcactac tcccatttta gtttgttcgt
     2821 ggcaaccttc tgcatacaat gtatctttta ctgttttctt tacaggatta ccagcttcgt
     2881 ttattacagg gtttccaaat tcatccaaca caggtacttg gatgtttttt gttaatacag
     2941 gatgcaattg attcgttacc gtaaattcgc cttccaacac atctaccccc gttgtaatat
     3001 tatacaattt tgctgaataa taagccgttg tatcggcttt taataaaccg caagcttctg
     3061 ttcttccttt tattttaaaa cgaactgagg ctgtcatatc tcctttacac acaatttttg
     3121 ttttcgacat cagctcagct tcaagcaatt cagcttgtgg aactaccata tccgcatgat
     3181 tggtaataca tccgttcgca tcttttactt gaacaaaata ggtagttgca ggtaaattat
     3241 caaaaacccc tacagtatta ctatacgaaa tacttccgtc aagcgaatta ataatgtata
     3301 ggtaaggagc atttcctcct tctgcttcaa ttctgatttt tccagtagat gctccatggc
     3361 atgatacaga ttgaatgaaa tctttggttg gattgaaagt gagaggaaga ggttgtgtga
     3421 tgcttacagt ttgccatgta ttgtcagcat gattattgac atcacgtaca tacaccgaat
     3481 aattagtagc aggtaaatta ataaatgtac tattcgattg aatagaattt acaaaattat
     3541 ctaaactata ttgaaatact cccgagcctc cagtggcaac tattgtaatt gatccatcgg
     3601 ataatccatt acaagatacg tggcttgtgt ttacactgtt aatctgaaca gctgcccaaa
     3661 gcggaacgat gcaaaaaagt gcaatcccca aaaagagctg acgtaaacga gatgttaccg
     3721 cttgatgttt gtttgaggat agaaaaaaag gtacgttttt tttcatgtac atattgattt
     3781 ttgtggtttg tgagaatatt attgttatca acttatctga aataataaaa caacgatagc
     3841 aaaagtaaaa aaaaatcttt cagaaacata ttttttacta aaaaaagaga atgaatttga
     3901 aaaaaataga atgtttttaa catttaaatg cgataacttg aaatatgtag gcttttttcg
     3961 tctattgatc tcctgttttg atgggagcat ggtgtgtatt tattgcaagt caattatggt
     4021 ttttgtaaaa aataatgtat cttcgcataa ttaaatgaat gatcgtatga aacagttttt
     4081 atgccttagt gcctttttct tattttctat ttcgttattt gctcaacaag cagtttttaa
     4141 aaatcgctat tatcccaacg gaaatttaat gtacgaaggg tattttgtag aagataaacc
     4201 cgtgggaaca ataaagcggt atcacgaaaa tgggaaactg aaagctatcc aacaatttgc
     4261 cacgaatgat actgtgaaag tggagtattt tgctggtgat ggttctcaat tggcgaaagg
     4321 aatttttgta aaaggacaaa aacaaggtat ttgggagtat ttttctaaag aaaaccgctt
     4381 ggtaatggta gagaattata cagaaggact gaagcaagga gagtcgttgg ttttttcgga
     4441 gtcgggtatg gtaattgacc gaatgcagta cgaaaataat gtgttgcatg gaaaacgtat
     4501 tcaatatcat ccgtatggca atattttggc aagctatttt tacaaaaatg gattgttaga
     4561 tggtcctttt gagtcatatt atgaaacggg tcaaaaagat gaagtaggct attatgtagc
     4621 caataaaaaa catggagaat ggaaaacata caatgatgat ggttcgttta cctctgtaga
     4681 atataaaaat gggaaagcta ctaatcagac agaactggat aaaaaactgc aagagacaat
     4741 ggataagaac gaacaaggcg atccaacaat aaaagatcct gctgattatc ttgaccaacc
     4801 agatgcgtat cttaatttgt ttcgctaata agtcggttat gttatgcctc cacacgcaca
     4861 catacgatta gttgaactga atagttttgt aaaaaaactg attgctgaca atacacaacc
     4921 tcagtggatt actgcagaaa ttagtgaagt aaacgaacat tattcaggtc attgctacct
     4981 cgagttgata gaaaaagatg atgctgatga gcatatcatt gccaaagcca aagcggtaat
     5041 ttgttcgttt acctaccgca tgataaaacc ttattttgag acaaccacac acgaacgcct
     5101 tatggctggt atgaaggtgt tggtaaaagt agaagtaagt tttcatgaag cctatggata
     5161 tagtttggta ataaaagata ttgatcctca atacacctta ggtgatatgg ctcgtaagcg
     5221 agccttgatt attcaacaat tgtatgccga aggggtgatg gatttaaaca aagagattga
     5281 tttgccatta cttgtgcaac gtattgctgt tatttcttcg cctggtgctg caggttatac
     5341 agatttttgt aatcaattac atggcaatgc gtttgggttt gtgttttatc atcgattgtt
     5401 tgctgctgtt atgcaaggca ctgaaacaga ggcttcgatt atcaatgccc tagaagctgt
     5461 gtatgagcac aaagaattgt ttgacgtggt ggtcatcata cgtggcggtg gagctacttc
     5521 tgatttgagt tggtttgata attataacat tgcctatcat tgcacacagt ttccgttgcc
     5581 tattttgagt gggattggtc atgataaaga tgtgtcggtg gtggatatgg tggcacatac
     5641 cagtttgaaa acgcccacgg ctgtggctca atttttggtg gattgtatgc aacagaccga
     5701 aagtaattta catactgtaa aagaagaaat aataaactat acagaacaag cccttgagag
     5761 ttggcaacaa cgattgcaac tagttgcatt gcagttgccc aacgctgtaa aacgaaaagt
     5821 agaacaagct tattatacgt taacatcttc catacaacac ttacagcatc gtagtaaaca
     5881 gaatcttacg caaaaagcat acaccttaga taaacaccag caatatgtac aattggtttc
     5941 gcctatacgc atatttgaaa aaggatatac gcttacacta aagaatggca aaccaattca
     6001 caatcaaata ctggcaaaag gagatgtggt tgaaacaatt tctttgcaaa aacgaatgaa
     6061 aagtactatt atagaaatac aaaatggatt gtaatattct cacaggaaga tatctatttc
     6121 gctccgctgg agcttatttg gtattgatat gttattttct attatgatgt agcccctacg
     6181 gggcataaga tatagcaatt atttacataa aaagcaacaa aacaccagct ccagaggagc
     6241 gaaataataa tagtattagc atagttttgg caatgcaagc tccgtaggag cggcatatta
     6301 ttaagtaaat agcgtttttt ttgaataaat aatattactc tgataactct gtgtaagatt
     6361 ctcacacgaa gaagacacga agatatctat ttcgctccgc tggagcttat ttggtattga
     6421 tatgttattt tctattatga tgtagcccct acggggcata agatatagca attatttaca
     6481 taaaaagcaa caaaacacca gctccagagg agcgaaataa taatagtatt agcatagttt
     6541 tggcaatgca agctccgtag gagcgacata ttattaagta aatagcgttt ttttgaataa
     6601 ataatattac tctgtgtcct tctgtgaaat aatctcttta aaaatataaa aaatgaaaaa
     6661 agaaaaaaat ttaacctacg atgaagctta cgcaaaacta gaagctattt tgcataaaat
     6721 cgaaagcaat gaattaggag tagaagaatt actagaaaat atcaaagaag ctactacact
     6781 gattgattac tgtaaaaacc gcctcaaaac aatagaccaa gaagtggcta aattaattga
     6841 aggcaagtag taaaaaattg agtgtgtatc aaattctcca tacacactca actagattta
     6901 tcttttcaag gtaaaatgtc ccgagagggt ttcgttaatt tctgctacat aaataatata
     6961 ccaatagtcg gtagttggca ttggattacc aagataattg ccgtcccatc ccaaatcaga
     7021 tcctttgtag cgaatcattt gtttgccaaa tctatcaaat atctggatgt cagcattggg
     7081 ataaaattct atacctacca cattccacct atcatttacc ccatcattat tgggtgagaa
     7141 gaatttatct ggaataatgg gcgtaggtac aattgtaaac atacggaacg atttacatcc
     7201 ctctgcattt tgaatttcta ccctatattc gccaatgggc aaatcaaata gttcgttgtg
     7261 ttgatatact gtatttccaa tgttaaaagt aaaaggagct gtaccacttt gctggtcgta
     7321 caaaattgtg gctgcacgtc ctgtggtaaa tatatctgtg ataacagggt ttggatgcac
     7381 cacgatgttt tgagtagccg tacatgtatg aggtgtaacg gcaaataccc cagcactttg
     7441 agttatcgat gccgtcaaag taactatata attacctgaa agtgttcctg atgcatcggt
     7501 tgttttagca cttgcattgc ttaatgacaa acccgttgat ggctgagtgg tccaagtagc
     7561 cgaccattca gtattggggt ttgcagttgc tcctagactg agtgattcac ccatacagat
     7621 agctgtttta ggggcttgta ttgctacatc tattacacgc aaattaagta ttaccgtact
     7681 atc
//
LOCUS       DAOSMY010000022         7640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9588_length_7640_cov_3.726829, whole genome shotgun sequence.
ACCESSION   DAOSMY010000022 DAOSMY010000000
VERSION     DAOSMY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9588_length_7640_cov_3.726829"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..970
                     /locus_tag="PLD76_01015"
     CDS             170..970
                     /locus_tag="PLD76_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26620.1"
                     /translation="MNITVDSDRVPVMSVNNEVLYADEIENILPANITGKDSVNFVEN
                     YKKNWAINILLYEKAEQNLRSTEEIDLLTEEYRKQLIINRYQQELISQKLKNISNDTL
                     INVYEREKTNFILEDPIIKGVFVQIPSKAPNQKQLKKWLTEMNDNTLEKIEKYCIQYA
                     SRYIFFRDDWTPYSQVDKLFPHHVDWNDPILTRGLVEQKDSISTYFLRITGKANPGTI
                     EPFEIALPKITNILSQREKMNYIKSFENSLYNNAIKKQKITFFLSSEE"
     gene            982..2337
                     /locus_tag="PLD76_01020"
     CDS             982..2337
                     /locus_tag="PLD76_01020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU26621.1"
                     /translation="MLKKITLLFVVCIFSIGVFSQNYIVDQVVWIVGDEAILQSEIET
                     EILRLKYEKQKTPESPECVIAEQLALQKLFLAQAKIDSITISEATINQQVESRIAYFT
                     EQLGSKEKVEAYFKKPLSKMREELKAMLQDQLMVQEMQQKITKDIKITPSEVTAFYEN
                     IPKDSLPIIPEQVEIQLITLSPTISVAEVERVKSKLREFRERIESGDIQFSTLAILYS
                     EDRVSALQGGEIGFRSRSMLVPEYANTAFSLTDPQKVSRIVETEYGFHIIQLIAKQDD
                     QVNSRHILMRPKISLEDKNLASQRLDSIAQAIRTGSINFEKGVEIYSDKSSTKNSNGL
                     LINTKDGSTRFQYDELPQEIINQVSFLNIGEISKPFSFTNENGKEIVAIVKIHDKIKA
                     HKASISDDYQTLKMVYTNVKNTQAIQQWIESKIATTYNQLTPAYRTCNFSYPHWLKNR
                     E"
     gene            2393..3916
                     /locus_tag="PLD76_01025"
     CDS             2393..3916
                     /locus_tag="PLD76_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOU26622.1"
                     /translation="MKNNVSIIVFLYVMCIVLCYNANVTAQNKTTVFLENAETLSFDQ
                     NVRNNAQLLQGNVRFRHDKTLMLCDNAYFYEKTNSLDAFGNVKIIQGDTLFIYGNTLY
                     YNGNTRLAQMRNNVRMINRDAVLTTQKLDYDTYSNIGYYTDFGTLTDKKNVLTSKEGE
                     YLTKQNIIKFKKNVVLKNAEFTLYSDTLHYHTYNSLATIVGPTTIISDSTRIYAEKGW
                     YNTSTEDTKLTKNGKIQHKNGKSIQGDSIFYNKKYATIEAFSNTLLKDSVQQISVLGN
                     YGKYFEQNEKGYVTKKATFREHSTADTLFLSADSLLYESIKGDFSVKAFRNVRFWQTD
                     FQGTCDSLTYNQTDSLLTMYHNPILWNEENQISGKVITLKMTGDKVDHFIVEEDAFIL
                     SEENPEEFNQLSGRKIIGYILNDKIRKIEIIGNAQSIYYVKDGESAIGTNRAESALLT
                     IFLNDSNQAEKIVMSPQSGGILYPTFQKNISIIRLSNFLKQNNNRPKNQYDIYTNRE"
     gene            complement(3956..5308)
                     /locus_tag="PLD76_01030"
     CDS             complement(3956..5308)
                     /locus_tag="PLD76_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOU26623.1"
                     /translation="MKTTKKIVLNENDMPREWYNIVADMHTKPMPPLHPVTKQPLAPA
                     DLEPIFAKELIKQEVSSERWIEIPEEVRDLYRIYRPTPLVRASGLEKALDTPAKIYFK
                     NESVSPVGSHKLNSAIPQAYYNKIEGITSITTETGAGQWGSAMSVACQHFGINLQVFM
                     VKISYEQKPYRKSVMNTYGANVVASPSNLTEVGRRMQQQFPGTNGSLGMAISEAMELA
                     MKDEKTKYTLGSVLNHVTLHQTIIGLEAEKQMEIAGDYPDVVIGCFGGGSNFSGIAFP
                     FLRHKLTEGKDIRLIAVEPASCPKLTRGKFLYDYGDTEGFTPLLPMYTLGHNFEPAEI
                     HAGGLRYHGAGTLVSQLRKDGLIEAEAIKQNETFEAGVLFARAEGIVPAPESTHAIAM
                     TIREALKAKEEGKERVILFNLSGHGLIDMAAYDKYLSGSLSNYEVSDAEIEKSLAGLK
                     "
     gene            complement(5648..6661)
                     /locus_tag="PLD76_01035"
     CDS             complement(5648..6661)
                     /locus_tag="PLD76_01035"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU26624.1"
                     /translation="MEKKIKAVINGIGGYVPEAILDNEELSRMVDTSDEWIMSRIGIK
                     TRHVLGERRGSAYMGIKAVQDLIEKTSLDTKDVDLLICCTTTPSFVFPSNASLICGAL
                     GITSAYGYDLAAACSGFIFGLDTCATMIESGKYKKIILVCSEKMTAITNYEDRNTCPL
                     FGDGAAAVLIEPTTEDVGVVDSILRIDGLGKENLHLKAGGSLHPASIETVTNKEHFIY
                     QEGQAVFKRAVSDMSAVAEEIMIKNALTSDDIAWLVPHQANIRIIEAVAKRMSLPREK
                     TLINIEHYGNTSAASIPLCLWDYESRLRKGDILVLAAFGAGFSWGSVVLKWGYDIEHS
                     KKM"
     gene            complement(6747..6932)
                     /gene="rpmF"
                     /locus_tag="PLD76_01040"
     CDS             complement(6747..6932)
                     /gene="rpmF"
                     /locus_tag="PLD76_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU26625.1"
                     /translation="MAHPKRKQSKTRTAKRRTHDKAVPATMAVCSNCGAMHIYHTICG
                     ECGYYRGKLAIEKTVNA"
     gene            complement(6962..7471)
                     /locus_tag="PLD76_01045"
     CDS             complement(6962..7471)
                     /locus_tag="PLD76_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOU26626.1"
                     /translation="MSHYIIPLKSIVSYNTTYDYELDESFFSAFVAEDGIIRGGNVKA
                     SIDVKKLNYSFELSIKLNGFVSVLCDRCLDVMEIQIDEDSVLVVKFGETYTEVDEELV
                     IVPEKEGVFNIAWYLYESLVLILPLQRIHQDGGCNKEMEKNLQKYSTDITVVNEKIDP
                     RWEALKNIK"
BASE COUNT         2718 a         1499 c         1076 g         2347 t
ORIGIN      
        1 ataaatgatt accaaaatta tctcgataaa caatggatag aggaactacg atcaaagtat
       61 cccatagtaa tagatactag cgttttaaat agtttaaagt ctaataatga ataaacgtta
      121 ttccttctat ctcattctta tcacctccac actattggga tgtaataagg tgaacattac
      181 agtcgactca gatcgggtgc ctgtcatgtc tgtaaataat gaagtgttat atgcagatga
      241 aatagaaaat atattacctg caaacataac aggaaaagat agtgttaact ttgtggaaaa
      301 ttataaaaaa aactgggcaa tcaatatttt attatacgaa aaagctgaac aaaacctacg
      361 ttcaacagaa gaaatagact tgttaaccga agaataccga aaacaactaa ttattaatag
      421 atatcagcaa gagcttattt ctcaaaaatt aaaaaacata agcaatgata cattaataaa
      481 cgtgtatgaa cgtgaaaaaa ctaatttcat tttagaagat ccaattataa aaggggtatt
      541 tgtacagata ccaagcaaag ctccaaacca aaaacaacta aaaaaatggt tgacagaaat
      601 gaatgataat actttagaaa aaatcgaaaa atactgtatt caatatgctt ctcggtatat
      661 attttttcga gacgactgga ctccatattc acaagttgat aagttatttc ctcatcatgt
      721 agactggaat gacccaattt taactcgtgg tttagttgag caaaaagaca gtatttcaac
      781 gtattttttg cgtattactg gaaaggctaa ccccggtaca attgaaccat ttgaaatcgc
      841 tctgcctaaa atcacaaata ttctctctca acgagagaaa atgaattaca ttaaaagttt
      901 tgagaattct ctttacaata acgcaattaa aaaacaaaaa atcaccttct tcctttcatc
      961 cgaagaataa tagccactat tatgttaaaa aaaatcaccc ttctgtttgt tgtatgtata
     1021 ttttcaatag gagtattctc tcaaaattat atcgtagatc aagtcgtatg gattgtagga
     1081 gatgaagcca tattacaatc agaaatagaa accgaaattc tacgtttaaa atatgaaaaa
     1141 caaaaaactc cagaaagtcc agagtgtgtt attgctgagc aactagccct acaaaaatta
     1201 tttttagcac aagctaaaat tgatagtatt acgatctctg aggctactat aaatcaacag
     1261 gtagaaagtc ggattgcgta ttttaccgaa caactcgggt caaaagaaaa agtagaagca
     1321 tattttaaaa aaccattatc taaaatgcga gaagaactaa aagcaatgct ccaagatcag
     1381 ctaatggttc aagaaatgca acaaaaaatt acgaaagata tcaaaattac accttcagaa
     1441 gtaaccgcat tttacgagaa cattcccaaa gatagtttac caataatacc agaacaagta
     1501 gaaattcagc ttattaccct atctcctact atatctgttg cagaagttga gcgagtaaaa
     1561 agcaaattac gcgaattccg cgaacgaatt gaatcaggag atattcagtt ttctaccttg
     1621 gctattttat actccgaaga tagggtatct gccttacaag ggggcgaaat tggttttcgt
     1681 tcgcgttcaa tgctagtgcc tgaatacgca aacactgcat tttctcttac tgaccctcaa
     1741 aaagtttctc gtatcgtcga aacagagtat ggttttcata ttattcaact tatagcaaaa
     1801 caggatgatc aagtcaatag tagacacata ctaatgcgtc caaaaatatc attggaagat
     1861 aaaaatcttg cttcccaacg tcttgatagt atcgctcaag caatacgtac aggctctata
     1921 aatttcgaaa aaggagtgga aatatattct gataaatctt ctacaaaaaa tagcaacgga
     1981 ttactcataa atactaagga tggtagcact cggtttcaat atgacgaatt accacaagaa
     2041 attataaatc aagtttcttt tctaaacatc ggggagattt ctaaaccatt tagctttacc
     2101 aatgaaaacg gtaaagaaat agttgctata gtaaaaatac atgataaaat taaggctcat
     2161 aaagcatcta ttagtgatga ctatcaaacc ttaaaaatgg tatacacgaa tgtaaaaaac
     2221 acacaagcaa tccaacaatg gatagaaagt aaaattgcaa caacatataa tcagttgacc
     2281 cctgcatatc gtacttgtaa cttttcatac ccccattggc taaaaaacag agaataatca
     2341 caacaaattg atgcaaaatg cacatatcca ttggcagatt aactacaaga atatgaaaaa
     2401 caatgtctct attatcgtgt ttttatatgt tatgtgtatt gtattgtgct acaacgctaa
     2461 cgtaactgct caaaataaaa cgactgtttt tctagagaat gctgaaacgc tatcttttga
     2521 tcaaaatgtt cgaaataacg ctcaacttct acaaggaaat gttcgattta gacatgataa
     2581 aacgttgatg ctctgcgaca acgcatattt ttatgaaaaa accaattcat tggatgcttt
     2641 tggtaatgtg aaaattatac aaggagatac tctttttatt tatggaaata ctctttatta
     2701 taacggcaat acacgtctag cccaaatgag aaataacgta cgaatgatta accgcgatgc
     2761 cgtgctaact acacaaaaac tcgactacga cacatattca aacattggct actacaccga
     2821 ttttggcaca ttaactgata aaaaaaatgt attaacatca aaagaaggcg aatacttaac
     2881 caaacaaaac ataatcaaat ttaaaaaaaa tgttgtgcta aaaaacgctg aatttacact
     2941 ctactcggac acattacact atcacacata taattcactg gctactattg ttggacccac
     3001 cacaatcatt tctgatagca ctcgtattta tgcagaaaaa ggatggtata atacctctac
     3061 tgaagacaca aaattaacta aaaatggtaa aattcagcat aaaaacggaa aaagcattca
     3121 aggagactct atcttttata acaaaaaata cgcaactata gaagcttttt caaacacctt
     3181 actaaaagat tctgttcaac aaatctccgt tttaggaaat tatgggaaat attttgaaca
     3241 aaacgaaaaa ggatacgtta cgaaaaaagc aacctttaga gaacattcca cagctgatac
     3301 attatttcta agtgccgatt ctttgttata cgaaagtatt aagggggatt tctccgttaa
     3361 agctttccga aatgtccgtt tttggcaaac agattttcaa ggaacatgtg attccctaac
     3421 ctacaatcaa accgactcgt tactcacaat gtaccacaat ccaatacttt ggaacgaaga
     3481 aaatcaaatc tctggaaaag taattacatt aaaaatgaca ggagacaaag tggatcattt
     3541 catagtagaa gaagatgctt ttatcctttc tgaagaaaac cccgaagaat tcaaccaact
     3601 atctggaaga aaaattatag gttacatact caacgataaa ataagaaaaa ttgaaataat
     3661 tgggaatgca caaagtatct attacgttaa agacggagaa agtgctatcg gcaccaatag
     3721 agcagaaagt gctctattaa ctatctttct aaatgattca aatcaagcag aaaaaatagt
     3781 tatgtctcct caatctggag gcatcttata cccaactttt caaaaaaata tatccattat
     3841 tcggctcagt aacttcttga aacaaaataa caatagacca aaaaatcagt acgatattta
     3901 tacaaataga gagtagccat tcaactactc tctatttgta taaaatgaat aattattact
     3961 tcaatccagc taaacttttt tcaatttcag catcagaaac ttcgtaatta ctcaaactac
     4021 cagacaaata tttatcgtat gcagccatat ctattaaccc atgtccagaa aggttaaaca
     4081 atataaccct ttctttgcct tcttcttttg cttttagagc ttctcgtata gtcatagcta
     4141 tagcatgtgt tgattctggt gctggaacaa taccctctgc acgtgcaaat aatacccctg
     4201 cttcaaaagt ctcattttgc tttattgcct ctgcctcaat taagccatct ttgcgtaact
     4261 gactaaccaa tgtacctgct ccatgataac gcaatcctcc ggcatgaatt tcggctggct
     4321 caaagttatg ccccaatgta tacatcggta ataaaggcgt aaacccttct gtatctccat
     4381 aatcatataa aaatttacca cgtgttaatt ttggacatga cgcaggttca actgctataa
     4441 gccgaatatc tttcccctct gttaacttat gacgtaaaaa aggaaacgca ataccagaaa
     4501 aattagaacc tccaccaaaa catccaatca ctacatctgg ataatcacct gcaatttcca
     4561 tttgtttttc tgcctccaaa cctataattg tttgatgcaa agttacatgg tttaatacac
     4621 tacccaatgt atacttagtc ttctcatctt tcatagctaa ttccatcgcc tcagaaatag
     4681 ccatccccaa actaccattt gttccaggaa actgttgttg catacgtctg ccaacctctg
     4741 ttaaattact gggagaagca actacatttg ctccatacgt attcattaca gatttgcgat
     4801 atggtttttg ttcataacta atctttacca taaacacttg caaattaatc ccaaaatgtt
     4861 gacaagcaac actcatggca ctgccccatt gaccagctcc agtttctgtg gtaatagagg
     4921 ttatgccctc gattttatta taataagctt gtggtatagc cgaattcaat ttatgtgatc
     4981 ctactgggct aacactctca ttcttaaaat aaattttcgc tggcgtatcc aaagctttct
     5041 ctaaacctga tgcacgtact aatggagttg gacgatatat acgatataag tctcgtactt
     5101 cttcgggaat ttcaatccat ctttcagatg acacctcttg tttaatcaat tcttttgcaa
     5161 aaattggttc taaatcagct ggagcaagtg gctgttttgt tactggatga agaggaggca
     5221 taggtttagt gtgcatatca gccacaatat tataccactc tcttggcata tcattctcat
     5281 tcaatacaat cttttttgtt gttttcatac aaacatatgt ttattagtaa attattaatt
     5341 caaatctctt taaataggaa aaggtgacaa aatgtagaat caccatcgaa aagctatctt
     5401 ctcataaaat agcttgaaat aaactagtat aaaacaataa tgaagtatca tataattttt
     5461 taaataacag aaaaaaaaag aaccataggt ggatttccta cggttcttat ctattttgta
     5521 caacacgcga cgaagcatcc accttattta taagtcagat tgccaccacc aattgttgct
     5581 gaatcttttt ttcatattat attttttgca aagtaaaaga atattattga attagcaaaa
     5641 aaatgtacta catttttttg ctatgctcta tatcatatcc ccatttaagc actacacttc
     5701 cccaagaaaa accagcccca aatgccgcta acaccaaaat atctcccttt cttaaacgag
     5761 actcgtaatc ccacaaacat aacggaattg atgccgctga cgtattccca taatgttcaa
     5821 tattaatcaa ggttttttct cttggtaaac tcattcgttt ggctactgct tcaataatac
     5881 gtatatttgc ttggtgtgga actaaccaag ctatatcatc agaagtaagt gcattcttta
     5941 tcataatctc ttctgctaca gctgacatat ccgacactgc tcttttaaat acagcttgcc
     6001 cttcttgata aataaaatgc tccttattag ttaccgtctc tatactagca ggatgtaaag
     6061 atcctccagc ttttaaatgt aggttctcct tccctaaacc atctatacgc aaaatagaat
     6121 cgactactcc aacatcttca gtagttggct ctataagtac agctgctgct ccatctccaa
     6181 acaaaggaca cgtattccta tcttcataat ttgtgatagc ggtcatcttt tcagaacaaa
     6241 ccaatattat ctttttatac ttcccagatt ctatcatagt ggcacaagta tccaagccaa
     6301 aaataaaacc tgaacaagca gccgccaaat catacccata agcagaagta atgcccaatg
     6361 ctccacaaat taatgatgca ttagatggga aaacaaaaga tggggtagtt gtacaacaga
     6421 ttaacaaatc aacatctttt gtgtccaaac tagtcttttc tattaaatct tgaacggctt
     6481 taatccccat ataagcagaa cctctacgtt ctcctaatac atggcgagtc ttaattccaa
     6541 tacgagacat tatccactca tcggaggtat caaccatcct acttaattcc tcgttatcta
     6601 atatagcctc aggaacataa cccccaattc cattgataac ggcttttatt tttttttcca
     6661 taatattctg tgctaaaaac aaaaactgcc ctaagtattt cgtcagggct tatttagttg
     6721 tattacaaag aaagcataat caaacgttat gcattaacag ttttttctat cgctaatttg
     6781 cctctataat atccacactc tccacaaata gtgtgataaa tatgcattgc accacaatta
     6841 gagcatactg ccattgtagc tggaaccgct ttatcatgcg ttcttctctt cgccgttctt
     6901 gttttcgact gttttctttt tggatgtgcc atctcttttt atataaatta attactactt
     6961 attattttat gttttttaat gcttcccatc gcgggtctat tttttcattt actacagtta
     7021 tatctgtact atacttttgt aaattctttt ccatctcctt attgcatcca ccatcttgat
     7081 gtattcgctg aagtggcaat attaatacca aagactcata caagtaccat gctatattga
     7141 acacaccttc tttctctggc actatcacca actcttcatc aacttctgta tacgtttctc
     7201 caaacttaac caccaacact gaatcttcat ctatttgtat ttccattaca tccagacatc
     7261 tatcacacaa aacagataca aatccgttta gcttaataga taattcaaac gaatagttca
     7321 gcttttttac atcaattgac gccttaacat tacctcctct aataatccca tcttctgcaa
     7381 caaaagcaga aaaaaaagac tcatctaact cataatcata cgtcgtattg tatgatacaa
     7441 tacttttgag aggaataata taatgactca aatctgacac tgtactagtt ttgataaaag
     7501 tgtggcaaag ttacaaaatt ttacgatact aaaacaaaaa ttacgtctaa aatatgtttt
     7561 ttttctcttt ttatttgtaa atagcttatt ttcaaaatta aacaaaagtc taattgcatc
     7621 ttccataatc catccttatt
//
LOCUS       DAOSMY010000023         7550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9746_length_7550_cov_4.311274, whole genome shotgun sequence.
ACCESSION   DAOSMY010000023 DAOSMY010000000
VERSION     DAOSMY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9746_length_7550_cov_4.311274"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..81
                     /gene="rpmG"
                     /locus_tag="PLD76_01050"
     CDS             <1..81
                     /gene="rpmG"
                     /locus_tag="PLD76_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU26627.1"
                     /translation="KNTTERLELKKYNPILKKVTVHKEIK"
     gene            94..246
                     /locus_tag="PLD76_01055"
     CDS             94..246
                     /locus_tag="PLD76_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOU26628.1"
                     /translation="MAKKTVASLKKTDGRAYAKVIKMVKSPKTGAYIFEEDIVLNDKV
                     KDFFGN"
     gene            387..1331
                     /gene="ftsY"
                     /locus_tag="PLD76_01060"
     CDS             387..1331
                     /gene="ftsY"
                     /locus_tag="PLD76_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU26629.1"
                     /translation="MGLFSFFSKDKKETLDKGLAKTKESVFSKITRAVVGKSKVDDEV
                     LDNLEEALLASDVGVETTLRIIERIEKRVADDKYMNTDELNGILKQEIIDLLAENNTD
                     FLQDTNEEGKPYVIMVVGVNGVGKTTTIGKLAYQFKQAGKSVYLGAADTFRAAAVEQL
                     CIWGERVNVPVIKQKMGADPASVAFDTISSAKANNADVVIIDTAGRLHNKVNLMHELS
                     KIKNVMQKVIPTAPHEVLLVLDGSTGQNAFEQAKQFTKATQITALAITKLDGTAKGGV
                     VIGISDQFKIPVKYIGLGEKMEDLQLFDKTAFVQSLLG"
     gene            1338..2639
                     /gene="rimO"
                     /locus_tag="PLD76_01065"
     CDS             1338..2639
                     /gene="rimO"
                     /locus_tag="PLD76_01065"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU26630.1"
                     /translation="MKKNHIDLITLGCSKNLVDTEKLMYQLKVAGYTVAHDAENPTGE
                     IVVINTCGFIGDAKEESIDMILAFAEKKKKRQINKLFVMGCLSQRYLQELAIEIPEVD
                     KFYGKFNYTDLLKELGKEYRFDCSLQRTITTPMHYSYIKISEGCNRTCSYCAIPLITG
                     KHQSRKIEDIEEEVRWLVKQGVKEFQLIAQDLSYYGLDNYKKHKLAELTNRLADIDGV
                     KWLRLHYLYPVDFPMDVLNVMRERDNVCNYLDIALQHISDNMLKKMRRGISSQQTYDL
                     IRQIREQVPGIHIRTTMLLGHPGETDEDVEALKECVRTMRFERLGAFAYSDEEATYSN
                     KHYVDNIPYEIKQQRVDEIMKIQQQISLEHNQAKIGKNFKVIVDSLQGDYYVARSEYD
                     SPEVDMEILLKAEKNHLQIGEFYTATIIGAEEFDLYAEIIK"
     gene            2677..2970
                     /locus_tag="PLD76_01070"
     CDS             2677..2970
                     /locus_tag="PLD76_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU26631.1"
                     /translation="MNNKELISELSKRLRVSQNDISNLLASTVSAFSTAFQQGNDVSL
                     QGFGIFQIRKKNERIIVNPSTNVRQIVPPKLTVIFKMSNTYKEKIKNIIPHGK"
     gene            2960..3970
                     /locus_tag="PLD76_01075"
     CDS             2960..3970
                     /locus_tag="PLD76_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU26632.1"
                     /translation="MENKKQSIQELVDIIATQTNFTKKNSEEFLKEFVHVLQEYLEKD
                     GFVKVKGLGAFKLVKNEERKSVNVTTGEAIIIPAHNKVSFIPDNAIKEAVNKPYAHLE
                     TITVDDDTKNSTIDAQPDNTVREEKSIQDEAKNNSIPNNTVIQQEETSNNSKKTKKNK
                     KKNPVAIVILLLLFHVAMAVSFYFYKTEIVSFFQRAVTIEENNLPQATIIDNQLSPVS
                     IVADTVVVVETDSMPDTTNIQQPDEIIATYTPELAQNAPIKEYVTVNEGSRLTWIAYK
                     AYGHKAFWVYIYDANRNQLKTPNDVKIGMELAIPELDASLVNPNDENCVIKALELAEK
                     YK"
     gene            4112..5106
                     /locus_tag="PLD76_01080"
                     /pseudo
     CDS             4112..5106
                     /locus_tag="PLD76_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            5125..5994
                     /locus_tag="PLD76_01085"
     CDS             5125..5994
                     /locus_tag="PLD76_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU26633.1"
                     /translation="METSEVIKRVRQVEIKTKGLSQNIFAGQYHTAFKGRGMAFSEVR
                     EYQYGDDIRNIDWNVTARFNHPYIKIFEEERELTVMLLIDVSGSREFGSVDKTKKNII
                     TEVAATLAFSAIQNNDKIGVLFFSDRIEKFIPPQKGKKHILHIIRELIDLQPKSRKTD
                     ISMVLKYFSNAIKKKCTAFILSDFIDTNDFEQAMLLANKKHDLVALQIYDKRETELPN
                     VGLLKIKDAETGEIVLVDSSNKAIRDLYQSVWNKKQYKLNQVFNRCRVDNVSIDTTED
                     YVKSLLKLFRLRS"
     gene            5998..6945
                     /locus_tag="PLD76_01090"
     CDS             5998..6945
                     /locus_tag="PLD76_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26634.1"
                     /translation="MYLNKFSWLIIFFFLSVPIAYCQTVTIDAELDSSSIIIGDQAIL
                     HIQITKPVAESIVIPQFTDTLVKGIDIISITKIDTTLVDDKQNIQFSIYLTSFEPGDY
                     TIPAIKFVGENDTYQTPLFPLLVKPFNINPDDEKLNDIKPILQMPFSWKTFFKWTAIV
                     LLVLALVAGIVYILVKYVFKKKIPLVKSKEEIIPPHIIAIQELDKIKAEKLWQQGKVK
                     EYYSAITDVIRVYLERRFDIPALEMTTHELVKLISTNPETLPIKDTLSELLHLSDIVK
                     FAKYIPLNSDNDLSLIHSYFIIDKTKIEPITEPTSEKDM"
     gene            6947..>7550
                     /locus_tag="PLD76_01095"
     CDS             6947..>7550
                     /locus_tag="PLD76_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU26635.1"
                     /translation="MVFQHPYILFLLFLLIPYIAWYIWKRRTLTPEIQFPTTGVLAQA
                     PKTPRMLLRHVPFVLRCLSVASIIFVIAQPQKRDSWKNETTEGIDMMLVMDISGSMLA
                     EDLRPNRLEAAKSVAVEFISGRTNDNIGLVAFSGKAFTQCPLTINHKELINLLLSLQT
                     GMIEDGTAIGEGLATAISRIKESPAVSKAIILLTDGANNRG"
BASE COUNT         2776 a         1081 c         1390 g         2303 t
ORIGIN      
        1 aaaaatacaa ctgaaagatt agaattaaaa aaatataatc caatcttgaa gaaagttaca
       61 gtacataaag aaattaaata aatagtataa gtcatggcaa agaaaacggt tgcgtctctt
      121 aaaaaaacag atggacgtgc atacgcaaaa gtgattaaaa tggtaaaatc tcccaaaaca
      181 ggagcttata tctttgaaga agatatcgtt ttaaatgaca aagtgaaaga tttttttggc
      241 aattaagcta atttaaacca tatttcaata acctcttaag tttttgcttg agaggttgtt
      301 ttgtatatat aggtatattt tttgtatctt tgtctatcca atttttgttt cacagcaatt
      361 cgtacattta taactatcaa aataatatgg gcttatttag ttttttttca aaagataaaa
      421 aagaaacctt agataaaggc ttagcaaaaa caaaagaaag tgttttctca aaaattacac
      481 gtgctgttgt tggtaaatca aaggttgacg acgaggtatt agataattta gaggaagctt
      541 tgcttgcatc tgatgtggga gtagaaacta cattacgtat aattgagcgt attgaaaaaa
      601 gggtggctga cgataaatat atgaatactg atgaattaaa tggtattttg aaacaagaaa
      661 ttattgattt attggcagaa aataatactg attttttaca agatacgaat gaagaaggta
      721 aaccatatgt gataatggtg gtgggagtaa atggtgtagg taaaaccacc acaataggca
      781 aacttgctta tcaatttaag caagcaggta agtcagttta cttaggtgct gccgatactt
      841 ttagagcagc tgcggttgag caattatgca tatggggaga acgggtaaat gtgcctgtta
      901 taaagcaaaa aatgggggca gaccccgcct ctgttgcttt tgatactata tcgtcagcta
      961 aggcaaataa tgctgatgtt gtgataattg atactgctgg tcgtttgcat aacaaagtga
     1021 atttgatgca cgaattatct aaaattaaaa atgtgatgca aaaagtaatt ccaacagcac
     1081 ctcatgaggt attgttagtg cttgatggtt ctactggaca gaatgctttt gagcaagcaa
     1141 aacaatttac aaaagctaca cagataactg ctttggctat cacaaaatta gacggaacag
     1201 ccaaaggcgg agttgttata ggaatttctg atcaatttaa gatacctgtt aaatatattg
     1261 gattgggtga aaaaatggaa gacttgcaat tgtttgacaa aacggctttt gtacaatctt
     1321 tattaggata atttgcgatg aaaaaaaatc atattgatct tattacctta gggtgttcaa
     1381 aaaatttagt tgatacagaa aaattaatgt atcaactaaa agtggcaggt tatacggtag
     1441 ctcatgatgc tgaaaatcca actggagaga ttgttgtaat taatacttgt ggatttattg
     1501 gagacgccaa agaagaaagt attgatatga ttctggcttt tgcggaaaag aaaaaaaaac
     1561 ggcaaataaa caaattattc gtaatgggtt gtctttcaca acgttattta caagaattgg
     1621 ctatagagat tccagaagta gataaatttt acggaaaatt taattataca gatttactca
     1681 aagaattggg caaagagtat cggttcgatt gtagcttgca acgtaccatc actacgccca
     1741 tgcattattc atatatcaag atatcagaag gatgtaatcg cacgtgctct tattgtgcga
     1801 tacctttgat aaccggcaaa caccaaagtc gaaaaataga agatatcgaa gaggaggtac
     1861 ggtggttagt aaagcaaggc gtaaaagaat ttcaattaat agcacaagat ttatcttatt
     1921 atggtcttga taattacaaa aaacacaaat tagctgaatt gacaaatcgg ttagctgata
     1981 tagatggtgt aaaatggtta cgtttacatt atttatatcc cgttgatttt ccgatggatg
     2041 tgctgaacgt aatgagggag cgagataatg tgtgcaatta tctagatatt gcactacagc
     2101 atatctcaga taatatgttg aaaaaaatga gaagaggaat atcttctcaa caaacctacg
     2161 atttgattcg ccaaattcga gaacaagtgc caggtattca tattcgtaca accatgttat
     2221 tgggtcatcc tggagaaacg gacgaagatg tagaagctct caaggagtgt gttagaacta
     2281 tgcgttttga gcggttagga gcatttgctt attcagatga agaagctact tattctaaca
     2341 aacattacgt agataacatt ccttatgaaa taaaacaaca acgtgttgac gaaataatga
     2401 aaatccaaca acagatatct ttggagcata atcaagctaa aattggtaaa aatttcaagg
     2461 taatagtgga tagtttgcaa ggagactact atgtggcacg ttctgaatat gattctccag
     2521 aggtagatat ggaaatttta cttaaagcag agaaaaacca ccttcaaata ggagaatttt
     2581 atactgcaac aatcattggg gcagaagaat ttgatttgta tgctgaaata ataaaataaa
     2641 tctgtatatt tgtcgttgtc ttaaaactat attactgtga ataataaaga attaatcagc
     2701 gaattgtcta aacgtttacg tgtgtcgcaa aatgacataa gtaatttgtt ggcatctacc
     2761 gttagtgctt tttctactgc atttcagcaa ggaaatgatg tttcattaca agggtttggt
     2821 atatttcaaa taagaaaaaa gaacgaacga attattgtca atccgtctac taatgttcga
     2881 cagattgtgc caccaaaatt aacagtgatt tttaaaatga gtaacacata caaagaaaaa
     2941 attaaaaaca taatacctca tggaaaataa aaaacaatcc atacaggaat tagtcgatat
     3001 cattgccact caaacaaatt tcacaaaaaa gaattcagaa gaatttttaa aagaatttgt
     3061 tcatgtattg caagaatatc ttgaaaaaga tggttttgta aaagtgaaag gtttgggtgc
     3121 ttttaaatta gtaaaaaacg aagagcgaaa aagtgtaaat gtaacaacgg gagaagcaat
     3181 tatcatccct gcacataaca aagtttcctt tatcccagat aatgctataa aagaagccgt
     3241 aaataagcct tatgcacatc ttgaaacaat tactgttgat gatgatacaa aaaatagtac
     3301 tattgatgct caacctgata ataccgttag agaagaaaag tccatacagg atgaggcaaa
     3361 aaataatagt attcccaata acaccgtaat tcaacaagag gaaacctcaa ataatagcaa
     3421 aaaaacaaag aaaaataaaa agaaaaatcc tgttgctatc gttattttat tactcctttt
     3481 tcatgtagcc atggcagttt cattttattt ttacaaaact gaaatcgtgt ccttttttca
     3541 acgggcggta actattgaag aaaataacct tcctcaagct actattatag acaatcaact
     3601 ttctccagtt agcatagtag ctgatacggt tgtagtagtt gaaacagata gtatgccaga
     3661 taccacaaac atacaacaac ctgatgaaat aatagcaact tatacaccag agcttgctca
     3721 aaatgctcca atcaaggagt atgttacagt aaatgaagga agtcggttaa catggatagc
     3781 ctataaagcc tatgggcata aagctttttg ggtttatatt tatgatgcca atcgtaatca
     3841 attaaaaact cccaacgacg taaaaattgg gatggaattg gctatcccag aattagatgc
     3901 atcgttggtt aatcctaatg acgaaaattg tgttattaag gcacttgaat tagctgaaaa
     3961 atataagtaa taataagata tagaaagaaa gtcacgccat gaatgtgtgg ctttttttgt
     4021 ttaacaattt atcaaggttt ttaacaaggt tttaaaccaa aaatatcaga aaatagtgta
     4081 atttcgtatc cgtattaaaa caacccaaaa tatgagtgaa atagttgata ttagagaatt
     4141 aaatgcacgt attgaacaga aaagtgcttt tgtggatgtg ttaacaattg gaatgaacaa
     4201 aacaattgta ggacagaaac atttgataga atcattattg ataggtttac tgtctgatgg
     4261 acatatcttg cttgaagggg ttcctggttt agccaaaacc ttagccatca aaacacttgc
     4321 acaattaatt gatgctacat acagtcgcat tcagtttaca ccagatttgt tacctgctga
     4381 cgtagtgggt actatgattt atagtcaaaa gaaagagcaa tttgaagtaa gaaaggacca
     4441 atttttgcta attttatttt agcagacgaa ataaaccgtg ctccagcaaa agtgcaaagt
     4501 gcactacttg aggctatgca agaaagacaa gttacgttgg ctgaaacaac acacaagctg
     4561 ccttctcctt ttttggtatt agctactcaa aatcctattg aacaagaagg aacataccct
     4621 ttgccagaag ctcaggtaga tagatttatg ttaaaagtcg tagtgtcgta tcctaaaaaa
     4681 gaagaagaaa gattaattat tagacaaaat attggaccag agcaaaaagc ccttgtccct
     4741 gtgttaacta ttaaggatat tcttgatgcc cgttctattg taaaggagat atatattgac
     4801 gagaaaattg aaaaatatat tttagatatt gtttttgcaa cgcgtaatcc agaagagtat
     4861 ggtcttgcaa aattgaaaga catgattgca tttggcgttt cgccacgtgc gtctattaat
     4921 ttagcattag cagctagggc atatgcattt atcaaacatc gtggatatgt agtgccagaa
     4981 gatattcgag ctgtgtgtta tgatgtatta cgacacagaa taggattaag ttatgaagca
     5041 gaagcaacaa atttaactcc tgaagatatt attagcgaaa taataaatgc agttgaggta
     5101 ccttaatcta tcaacatcga attgatggaa acaagtgaag taataaaacg tgtacgacag
     5161 gtagagataa aaaccaaagg cttatctcaa aatatttttg cagggcagta tcatacagct
     5221 ttcaagggta ggggtatggc tttttcagaa gtaagagaat atcagtatgg cgatgatata
     5281 cgtaatattg attggaacgt aacagcccga tttaatcatc catatataaa aatttttgaa
     5341 gaagagagag aattaaccgt aatgttgctt attgatgtgt cgggtagccg tgaatttggg
     5401 tctgtagaca aaaccaaaaa aaatattatt accgaagtag ctgctacact tgctttttct
     5461 gctattcaga ataatgataa aattggggta ttattttttt cagatagaat tgaaaaattt
     5521 atcccgccac aaaaaggaaa gaaacatatt ttacatatta ttcgcgaatt aattgattta
     5581 caacctaaga gccgtaaaac tgatatttcg atggtgttga aatatttctc taatgcgatt
     5641 aagaaaaaat gtaccgcttt tattttgtcc gattttattg atacaaatga ttttgaacaa
     5701 gccatgttat tggcaaacaa aaaacatgat ttggtagcat tgcagatata tgataaaagg
     5761 gaaacagaat tacccaatgt gggcttgctt aaaataaaag atgccgaaac tggagaaatt
     5821 gtactagttg attcgtcaaa taaagccata agagacctat atcaatccgt ttggaataaa
     5881 aaacaatata agcttaatca ggtatttaat agatgtagag tagataatgt ttccattgat
     5941 accacagaag attatgtaaa atccttactt aaattattcc gtttaagatc ttaatgtatg
     6001 tatttaaata agtttagttg gttgattata tttttctttt tgagtgtgcc aatagcatat
     6061 tgtcaaacag ttacaattga tgctgagttg gattcttcta gtattataat tggagatcaa
     6121 gctattctac atatccagat aactaagcca gtagcagaat caattgtcat ccctcaattt
     6181 acggatactc tggtaaaagg tatcgatatt attagtataa ctaaaataga tacaacactt
     6241 gtagatgata agcaaaatat tcagttttca atttatttaa cttcttttga gccaggagat
     6301 tatactatac ctgctatcaa atttgtaggc gaaaacgata cgtaccaaac gccattattt
     6361 cctttgcttg taaaaccttt caacataaat cctgatgatg aaaaattgaa tgatataaag
     6421 cctattttac agatgccatt tagttggaaa acatttttta aatggacagc cattgtattg
     6481 cttgttcttg ctttagtagc aggtatagta tacattcttg taaaatacgt tttcaagaaa
     6541 aaaattccgt tagttaaatc taaagaagaa attattcctc ctcatatcat tgccatacaa
     6601 gaattggata aaattaaagc agaaaaatta tggcaacaag gaaaagtaaa agaatattat
     6661 tcagcaatta cagatgtgat aagggtatat ctcgaaaggc gatttgatat tccagcattg
     6721 gagatgacaa cacatgaatt agtaaaatta atttcaacta atcctgaaac actacctata
     6781 aaagatacgt tgagcgaatt attgcattta tctgatatag ttaagtttgc taaatatata
     6841 cctcttaaca gtgataatga tttgagttta atacacagtt attttataat tgataaaaca
     6901 aaaattgaac caataacaga accaacttca gaaaaagaca tgtaatatgg tatttcaaca
     6961 tccttatata ttatttttat tatttttact tataccttac attgcttggt atatatggaa
     7021 acgtcgtaca ttaactcctg aaatacagtt tcctaccaca ggtgttttag ctcaagcacc
     7081 aaaaacgcca cgaatgttat tgcgtcatgt tcctttcgtt ttacgttgct tatctgttgc
     7141 tagtattatt tttgtaatcg ctcaaccgca aaaacgagat agttggaaaa acgaaactac
     7201 agagggaata gatatgatgc ttgttatgga tatttccgga agtatgctag ctgaagattt
     7261 acgacccaat cggttggaag cagctaagag tgtggcagtc gaatttattt ctggaagaac
     7321 gaatgataat ataggtttgg tagcattttc tggtaaagct tttacacaat gtcccctaac
     7381 gattaatcac aaagaattga ttaatttatt attgtcttta caaacaggta tgattgaaga
     7441 tggaactgct ataggagaag gacttgcaac tgctataagt agaataaaag agagtccagc
     7501 ggtatccaaa gctatcattt tattaacaga tggagcaaat aatagaggcg
//
LOCUS       DAOSMY010000024         7507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9828_length_7507_cov_3.112319, whole genome shotgun sequence.
ACCESSION   DAOSMY010000024 DAOSMY010000000
VERSION     DAOSMY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9828_length_7507_cov_3.112319"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..526
                     /gene="trxA"
                     /locus_tag="PLD76_01100"
     CDS             212..526
                     /gene="trxA"
                     /locus_tag="PLD76_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU26636.1"
                     /translation="MAKEFTESNFEELLQNNVVVVADFWAEWCGPCRMITPIVEELAS
                     AYEGKVLIGKVNVDEEGDLATKYGIRNIPTVLFFKNGQIVDKQVGAAGKNVFEDKINA
                     LL"
     gene            complement(597..1139)
                     /locus_tag="PLD76_01105"
     CDS             complement(597..1139)
                     /locus_tag="PLD76_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU26637.1"
                     /translation="MKQEIIIAGFGGQGVLSMGKILAYSSLLEGKEVSWFPSYGPEQR
                     GGTANVTVIVSDNRISSPILNTFDVAIILNQQSLNKFESKVKPGGILIYDGYGISKEP
                     TRTDIFIYRINAMDTSIAMNAAKSFNMIILGGLLKVLPFVNIESIMQGLKNSLPERHH
                     HMLPTNEAAIRKGMEIIEKI"
     gene            complement(1157..1918)
                     /locus_tag="PLD76_01110"
     CDS             complement(1157..1918)
                     /locus_tag="PLD76_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU26638.1"
                     /translation="MTTKDIIKPENLVYAKPSVMNNNTMHYCPGCSHGVIHKLIAEVI
                     GEMGIQDKAIGVAPVGCAVFAYNYLDIDWHEAAHGRAPAVATGIKRLLPDNYVFTYQG
                     DGDLAAIGTAETIHACNRGENIAIFFVNNGIYGMTGGQMAPTTLLGMKTSTCPSGRQE
                     DLNGFPLPITTLVAQLEGTRYVTRQSVHTANAVKKAKKAIRKALENSAEGKGTSFVEF
                     VSTCNAGWKMSPAEANTWMEKNMIPVYPIGDLKDK"
     gene            complement(1946..3028)
                     /locus_tag="PLD76_01115"
     CDS             complement(1946..3028)
                     /locus_tag="PLD76_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU26639.1"
                     /translation="MEQDIKLLKGNEAVAEAAIRCGCDGYFGYPITPQSEIMETLMQL
                     EPWKTTGMVVLQAESEVAAINMVYGGAGCGKRVMTSSSSPGMSLKQEGLSYMAGADLP
                     GLVVSVMRGGPGLGTIQSSQADYFQTVKGGGHGDYKMITLAPASVQEMADFTVLGFEL
                     AFKYNIVSVVLADGIIGQMMEKVQLPPYQKRLTNDEIREKYPWATLGKPANRKPNVIT
                     SLELDSALMEQRNLNLQKKYKEIEKNEVRYEEIACEDAEYLLVAFGSSARICQKTVEL
                     AREKGIKLGLFRPITLFPFPTNEIYAYTNTVKAILTVELNAGQMVEDVRLAVNGTIPV
                     EHYGRMGGIVPTAEEILEVTEKLIAK"
     gene            complement(3033..3260)
                     /locus_tag="PLD76_01120"
     CDS             complement(3033..3260)
                     /locus_tag="PLD76_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU26640.1"
                     /translation="MAKIKGRVVIDNERCKGCDLCAVSCPTDVLAMSKSVNSRGYNFS
                     HAVNEEACIGCASCALVCPDACITVYKVKVE"
     gene            complement(3358..3696)
                     /locus_tag="PLD76_01125"
     CDS             complement(3358..3696)
                     /locus_tag="PLD76_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26641.1"
                     /translation="MKPIKNLEPFSKWILRLSVLCFLGTIHYKDLQKIDYQDYNNIIM
                     VVYILSSLLLVLGGLQKKATLTIISALFLTLISFYQIYLSFNGNFLQHSIYQHLMISG
                     IGLFFISKGN"
     gene            complement(3724..5040)
                     /locus_tag="PLD76_01130"
     CDS             complement(3724..5040)
                     /locus_tag="PLD76_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU26642.1"
                     /translation="MQHAPIDPAIVQDVIDKLKLSDFSKATIREIVTIANAAETRTGK
                     KYIRMEMGVPGIDASEIGVKAEIKALEQGVAARYPMLEGIPQLKEESSRFIKAFVDVD
                     INPACCVPAIGSMNGTYASFLVAGQCHKEKDTVLFLDPGFPVQKMQLQVLGYKSVAFD
                     VYDYRGKKLQAKMEEFLKQGNISTIIYSNPNNPSWICLNQEELEIIGLLATKYDAIVL
                     EDLAYFAMDFRHDLGHPFEPPYQPSVAKYTDNYILLISGSKAFSYAGQRIGIIAMSPT
                     LYHRSYPHLIARYGVGEFGNILISRVLYSLSAGVTHSTQYALAAMFKAASDGSFHFLE
                     SVKEYGRRATLLKEIFARHHFSIVYDTDLKEPIADGFYFTVAYPHMKGCDLMKNLLFY
                     GISAISLNTTGSQQEGLRICTSFINDAQYDELDYRLSCFNKDFPIS"
     gene            5157..6236
                     /locus_tag="PLD76_01135"
     CDS             5157..6236
                     /locus_tag="PLD76_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU26643.1"
                     /translation="MKDKLVTLAIHTIEQALQLQHRLEKEGIPSFIKKIRSISTGVRV
                     RVREVDLQKAIYLVERIKSTSNEHIEFKSPFILVPIDFSEHAIHAAKLAFYLAENLHS
                     SIVFLHVETYVNVATFPTLLSSADEKSRLVTKKIKERNEAIIDVENRFKSLIANQLIP
                     NIHFNFELKNGIPEEEIISFAMLYKPLLIVMGTRSKANKEQEIIGSVTAEVIERSLVP
                     VFVLPQHTHLQTIQQIKHIGFATNFDKKELVAFEKLMYFVQKLQFKVSFIHVDDKEDI
                     QNKNKLDELKSYFLTVYPHLQLQFSMLISEKSTLKELENYIRDQKIDILAIRNHKRGM
                     FVRLFAPSLARKLVYQAKNPMIVFY"
     gene            complement(6327..>7507)
                     /locus_tag="PLD76_01140"
     CDS             complement(6327..>7507)
                     /locus_tag="PLD76_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU26644.1"
                     /translation="KIDIEQLKFEADKAEREGDYGKVAEIRYGKIKQKEEEIANFQQK
                     LVSLQADHPIIKEEVDSEDIADIVSRWTGIPVSKMVQSEKDKLLHLEAELHKRVIGQH
                     EAITAIADAVRRSRAGLKDPKRPIGSFIFLGTTGVGKTELAKALAEFLFDDENMMTRI
                     DMSEYQEKFSVTRLIGSPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHPDVFNIL
                     LQVLDDGRLTDNKGRIVNFKNTIIIMTSNIGSSLIRENFEHITPSNRDNVIENTKNEI
                     FELLKKSIKPEFLNRIDDLIMFTPLSEEEIHQIVGLQIKLIANMLHKNDIDFSVTDKA
                     IDLIAKIGYDPQFGARPIKRVLQRYVLNELSKQMIAEKIDKSKPIIVDVEAGELIFKN
                     "
BASE COUNT         2370 a         1340 c         1211 g         2586 t
ORIGIN      
        1 gttgaagtta tagatcccga aacacgttgg attgttaaat tgaataaaaa agatgggaaa
       61 ataacagtca ataaaccaat ctttcatttt cttaaagaaa agaaagaaaa tcaaatactt
      121 gattttatca tttcttaata ggattttaca ttgaaattat atacatttgc acatatatat
      181 ttatatcaac taataataaa aatagacaaa catggcaaaa gaatttacag aatcaaattt
      241 tgaagagtta ttacaaaata acgtagtcgt agtagcagat ttttgggcag aatggtgtgg
      301 accctgtcgt atgattaccc ctatcgttga agaactcgct agtgcttacg aaggaaaagt
      361 attgattggg aaagttaatg ttgatgaaga aggagactta gctaccaaat atggtatccg
      421 aaatattcct actgtattgt tttttaaaaa tggtcaaatt gttgataagc aagtaggtgc
      481 ggcaggtaaa aatgtatttg aagataaaat caacgcacta ttgtaatacc tatatctttt
      541 tgggaaaaaa agggagtttc atgttagtat gaactccctt tttctattta aaaaaattat
      601 attttctcaa taatctccat accttttcgt atagcagcct cattcgtagg caacatatgg
      661 tgatgacgtt caggtaatga gttttttaag ccttgcatta tactttctat attaacaaat
      721 ggcaatactt taagtaatcc tcctaatata atcatattga atgatttggc agcattcatt
      781 gctattgaag tatccattgc attaatgcga tatatgaata tatcagtgcg agtgggttct
      841 ttgcttatac catacccgtc gtatataagt attcctccag gttttacttt actttcaaat
      901 ttatttagtg attgttggtt gaggataatt gctacatcaa aagtattaag aataggagag
      961 ctaatacgat tatcacttac aataactgta acatttgctg taccaccacg ttgttctggt
     1021 ccatatgatg gaaaccaact tacttctttc ccctcaagta aactcgaata agcaagaatt
     1081 tttcccattg ataatactcc ttgtccacca aaacctgcta taataatttc ttgtttcatg
     1141 tgtcaaatgt tgtttgttat ttatctttta aatcgccaat tggataaact ggtatcatgt
     1201 ttttttccat ccatgtattg gcttcagctg gtgacatttt ccaaccagca ttgcatgtag
     1261 aaacaaattc tacaaatgat gttcctttgc cttctgctga attttcaagt gcttttcgga
     1321 tagctttttt cgccttcttt acagcatttg ctgtgtgtac actttgtcgt gttacgtagc
     1381 gtgtaccttc taattgggct actaaagtag taataggaag aggaaaacca tttaaatctt
     1441 cttgtcttcc agatggacat gtagaggttt tcattcctaa taaggtagta ggagccattt
     1501 gtccccccgt cataccatag atgccattgt ttacaaaaaa gatagcaata ttttcacctc
     1561 tattacatgc atgtattgtt tcggcagtac caatggcagc caaatcgcca tcgccttgat
     1621 aggtaaatac ataattatca ggcaataagc gtttaatacc tgttgctaca gcaggagccc
     1681 taccatgagc agcctcatgc caatcaatat ctaaataatt gtaggcaaat acagcacaac
     1741 caaccggagc tactccaatt gccttgtctt gaatacccat ttcgccaatt acttctgcaa
     1801 ttaatttgtg aataacgcca tgactacaac cagggcaata gtgcatagta ttattattca
     1861 taaccgatgg tttggcatat accaaatttt cgggttttat tatatctttt gttgtcataa
     1921 tatcattttg ttttaaaatg aataattact ttgcaattaa cttttcagtt acttccaaaa
     1981 tttcttctgc tgtaggtaca ataccaccca ttcgaccgta gtgttctact ggaattgttc
     2041 catttaccgc caaacgtaca tcttctacca tttgaccagc atttaattct actgttaaga
     2101 tggcttttac tgtgttagta tatgcgtaaa tttcgttggt tggaaaaggg aataaggtga
     2161 ttggtcggaa caatccaagt ttaatacctt tttctcttgc aagttccact gttttctgac
     2221 agatacgagc cgaagagcca aaagcaacta ataaatattc agcatcttcg caagctattt
     2281 cttcatacct tacttcattt ttttctattt ctttatactt cttttgtaag tttaaatttc
     2341 gttgctccat tagagcagaa tctaactcca aagaggtaat aacattaggc tttctattgg
     2401 caggttttcc taaagtagcc caaggatatt tttctctgat ttcatcgttt gttaaacgtt
     2461 tttgataggg tggaagctgt actttttcca tcatttgacc aataatacca tcagccaaca
     2521 ccacagatac aatattatat ttaaatgcta attcaaaccc taatactgtg aaatcagcca
     2581 tttcttgtac agaggcagga gctaatgtta tcattttgta gtcaccatga ccaccacctt
     2641 taactgtttg aaaataatca gcttgactag attgtatagt tcctaaacca ggaccaccgc
     2701 gcattacgct tactactaat ccaggtaaat cggcaccagc catataggac agaccttctt
     2761 gttttaggct catacccggg ctagatgatg atgtcattac tctttttcca cagcctgcac
     2821 caccgtatac catattaatg gcagccactt cgctttcggc ttgtaacacc accattcctg
     2881 tagttttcca aggctctagt tgcattaatg tttccataat ttcagactgg ggtgtaatag
     2941 ggtagccaaa atacccatcg catccacaac gtatagccgc ttcggcaaca gcttcgttgc
     3001 cttttaataa ttttatgtct tgctccatgt tgttattcta cttttacttt gtaaactgtt
     3061 atacatgcat ctggacacac gagtgcacaa ctggcacaac caatacatgc ttcttcatta
     3121 actgcatgag aaaagttata tcctctgctg tttacacttt tagacatcgc caacacatct
     3181 gtggggcacg aaacagcaca caagtcgcat cctttacaac gctcattgtc gataactact
     3241 ctacctttta tttttgccat tatagtgaac aattttggtt attttgaaag tacaaagtta
     3301 ttttattttt cattcaataa gtttttaaga acgttaaaga atggtaacaa cttgtatcta
     3361 gtttccttta gatataaaaa atagaccaat tccacttatc attaggtgtt gataaataga
     3421 gtgttgcaaa aaatttccgt taaatgatag gtaaatttgg tagaaggaaa taagtgttaa
     3481 aaatagagca cttattattg tcagtgttgc ttttttttgt aatccaccta atacaagtaa
     3541 caaactacta agaatatata ctaccataat gatattgtta taatcttggt agtcaatttt
     3601 ttgtaaatct ttatagtgga ttgttcctaa aaagcatagg acacttaatc gcaatatcca
     3661 ctttgaaaaa ggttctaggt ttttaattgg tttcatagtt gatgaaataa atgggtaaaa
     3721 tgattatgaa atgggaaagt ctttgttaaa acaggataaa cgatagtcta attcgtcgta
     3781 ttgagcatca ttgataaatg aggtacatat acgtaatcct tcttgttggc ttcctgttgt
     3841 attgagagaa atggcactga taccataaaa aagtaaattt ttcatcaaat cacaaccttt
     3901 catatgtgga tatgctactg tgaaataaaa tccgtcagct attggctctt ttaaatctgt
     3961 atcatacaca atagaaaaat ggtgtctagc aaatatttct ttcagtaatg ttgctcttcg
     4021 tccgtattct ttaacgcttt ctaaaaaatg aaaagagcca tcacttgctg ccttaaacat
     4081 ggctgctaat gcatattgag tagagtgggt tactccagcc gaaagagaat ataaaaccct
     4141 acttataaga atattaccaa attcacctac accatatctt gctataagat gtggataaga
     4201 acgatggtat agggtcggcg acatggcaat aataccgata cgttgacctg cgtaactaaa
     4261 agctttagag ccagaaatta ataaaatata attatctgta tatttagcta ctgatggttg
     4321 atatggtggc tcgaatggat gtcctaggtc gtgcctaaaa tccattgcaa aataggctaa
     4381 atcttctaat acgatggcat catatttagt tgctaataaa ccaataattt ccaattcttc
     4441 ttgatttaaa catatccaag atggattgtt tggatttgag tatataatgg tggaaatatt
     4501 tccttgtttg agaaattctt ccatcttagc ctgtaatttt ttgcctctgt aatcatatac
     4561 atcaaaagca actgatttat aaccaagcac ttgcaattgc atcttttgta caggaaaacc
     4621 tgggtcgaga aacagtacag tatctttttc tttgtggcac tgaccagcaa ctaaaaagga
     4681 ggcatatgtg ccattcatag aaccaattgc aggaacacaa caagcaggat tgatatcaac
     4741 atcaacaaag gctttgatga aacgagaaga ttcctctttt agttgtggaa taccttccag
     4801 cattggatag cgagctgcta cgccttgctc taatgcttta atttctgctt taacaccaat
     4861 ttcggaggca tcaataccag gtacacccat ttccatacgt atgtactttt ttcccgtacg
     4921 tgtttcggca gcattggcga tagttacaat ttctcgtata gtagccttac taaaatcaga
     4981 taactttagc ttatcaataa catcttgcac tattgcagga tcaatgggag cgtgttgcat
     5041 acaaatttac tctaaggatt atgttctttt ttaatgctac aaatttagaa aaaatatttt
     5101 attttttgct gttttaaaaa atatccatta cttttactga ctaactatta ctatatatga
     5161 aagataaatt agttacacta gcaattcata caatagaaca agcattacaa ttgcaacata
     5221 gactcgaaaa agaaggtatt ccatctttta taaagaaaat acggagtatt tctacgggag
     5281 tacgtgtacg agtacgtgaa gttgatttgc aaaaagcaat ttatttagta gaaagaatca
     5341 aatcaacctc aaatgaacat attgaattta aatctccatt tattttagtt cccatcgatt
     5401 ttagtgaaca tgctattcat gccgctaaac tagcatttta tttagcggaa aacttacatt
     5461 catctatcgt ttttttgcat gtagaaacat acgtaaatgt agcaactttt ccaactttat
     5521 tatcctcagc agatgaaaaa tcacggttgg taacaaaaaa aattaaagaa cgaaacgaag
     5581 ctataataga tgtggaaaat cgttttaaat ctttgattgc aaatcaatta ataccaaata
     5641 ttcattttaa ttttgaactt aagaatggaa ttcctgaaga agaaatcatc tcttttgcaa
     5701 tgctttataa gccgttactc attgttatgg gaacacgaag caaagctaat aaagaacaag
     5761 agattatcgg gagtgtaaca gcagaggtaa tagaacgtag tttggtacca gtttttgtat
     5821 tgccacagca tacacattta caaactatac aacagatcaa acatattgga tttgccacta
     5881 attttgataa aaaagagttg gttgcttttg aaaaattgat gtattttgtg caaaaattac
     5941 aatttaaagt tagctttatt catgtagatg ataaagaaga tatccaaaat aaaaataaat
     6001 tggatgaatt aaaatcctat tttcttactg tttatcctca tttacaactt cagttttcaa
     6061 tgcttataag tgaaaaatcg acccttaaag aactagagaa ttatatccgt gaccaaaaaa
     6121 tagatatatt ggcaatacgc aatcataaga gggggatgtt tgtacgtttg tttgcaccta
     6181 gtttggctcg caaactagtt tatcaggcga aaaatccgat gattgtattt tattaattaa
     6241 attgtttcat aattaatatt atgttaaata ggatatatcc aaataaagcg acagttgtat
     6301 ttttgtcgct ttatttatta gtactattag tttttaaaaa ttagctcccc tgcttctaca
     6361 tctacaataa ttggtttaga tttatcaatt ttttcggcaa tcatttgttt cgacaattcg
     6421 ttgagtacat atcgttgtaa tactcttttt attggtcgtg ctccaaattg tggatcgtag
     6481 ccaatttttg ctattaaatc tatcgcttta tctgttacag aaaaatctat atcatttttg
     6541 tgaagcatat tggcaattaa ttttatttgt aagccaacaa tctgatgtat ttcctcttct
     6601 gacaatggcg taaacataat taaatcatct atacgattca agaattcggg tttgatactc
     6661 ttttttaata gctcaaaaat ctcattcttc gtattttcta tcacattatc acgattggat
     6721 ggagtgatat gttcaaaatt ctcacgtatt aaggacgaac ctatatttga tgtcataatg
     6781 ataattgtgt ttttaaaatt aacgatacgt cctttgttat cagtcaagcg accatcatct
     6841 aatacttgca acaatatgtt aaatacatca ggatgtgctt tctcaatttc gtcgaataat
     6901 acgacagaat atggcttgcg gcgaatggct tctgttagct gaccaccttc gtcgtagcct
     6961 acatatcctg gaggtgaacc aattaatcgt gttactgaga atttttcttg gtattcactc
     7021 atatcaatgc gagtcatcat attttcatca tcaaacaaaa actcagctaa agccttggct
     7081 aactctgttt taccaacgcc tgtagtacct aagaaaataa atgagcctat ggggcgtttt
     7141 gggtctttga gacctgccct actccttcgc acagcatctg ctatggctgt aatggcttcg
     7201 tgttgaccaa taacacgttt gtgcaattct gcttccaaat gcaagagttt atccttttca
     7261 ctttgtacca ttttgcttac aggaatacct gtccagcggc tcacaatatc agcaatatcc
     7321 tcactatcaa cttcttcttt tattattgga tgatcagctt gtagtgatac caacttttgt
     7381 tgaaaattgg ctatttcttc ttctttttgt ttaattttcc cgtacctaat ttctgctact
     7441 ttgccatagt cgccttcacg ttcagctttg tccgcttcaa atttcaattg ttcaatatca
     7501 attttat
//
LOCUS       DAOSMY010000025         7491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_9861_length_7491_cov_3.349112, whole genome shotgun sequence.
ACCESSION   DAOSMY010000025 DAOSMY010000000
VERSION     DAOSMY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9861_length_7491_cov_3.349112"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..404
                     /locus_tag="PLD76_01145"
     CDS             195..404
                     /locus_tag="PLD76_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26645.1"
                     /translation="MKKLLCPQCRIHRFRVFNAARESVVVTVNEHYEVIPIHEGVSLE
                     GYFTDELHCLGCSWIGSPKALLNRY"
     gene            complement(465..1169)
                     /locus_tag="PLD76_01150"
     CDS             complement(465..1169)
                     /locus_tag="PLD76_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26646.1"
                     /translation="MTTKKYILLIASLLLSTAYVAANKQTLSTIELSVEKGKSYNYPT
                     FALWLESAGGKFVQELYVTQSLATGIYNNADAGDKRWKREKGEARRPSTLPYFLHKRD
                     IQAPDGTYLPTPEQPIPDAYTGATPRQSDVMTIVLDRQLYGKYRIVFEINQPWDFNRV
                     WYNNRYPNNTDYLLSGQPSLVYMVEIDTNNLQQEYTLNPIGHGHPYGKTGTLFTDISG
                     ITTAKNIFKSIKVKVY"
     gene            complement(1211..3628)
                     /locus_tag="PLD76_01155"
     CDS             complement(1211..3628)
                     /locus_tag="PLD76_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU26647.1"
                     /translation="MNIYTLKTILIATLLAAIGFSGFAQAEQGGALRGRVYNPDNNQP
                     VDFAYVIIDGTTIGTITDLDGNFAIFGLKPGYVRVAVNSLGFDPVVTKEILITNANTP
                     YIEIEMKPSNIQLDDVVVEASRFRQREESPLSLRSIGIKEIEKSPGGNRDISKVIQSL
                     PGVGATPANRNDIIVRGGGANENRFFLDGVEIPNINHFATQGSSGGPAGIINVDFVRE
                     LDFFAGAFPANRGNALSSVLDFRLIDGNKDRLKTRATVGASDLALTLDGPIGDKSSFV
                     FSVRRSYLEFLFDLIGLPFLPTYNDIQFKHKIRFNQNNELSFIGLGAFDVNRLNLSAN
                     ETESQRFILKYLPTNDQWTYTVGAVYRNFFKNGNQTLVLSRNHLNNVSQKYQDNIEAP
                     TNLNFDYASNEIETKIRYEYVTRQGAWKFLYGAGAEYNQYTNYTYNKLFFNAQVVEFD
                     YETELDFYSYSAFTQISRNLLAERLLVSAGARIDGNTYSEQMSNPLEQFSPRISASYA
                     LTNKWSLNANTGRYYQRPAYTTMGYRDIAGNLINKDNRLRYKYTDHFVVGVENKPNPN
                     SRFTVEGFYKLYNDYPFSISDSVAISSKSADFDVFGAEAVLSIGEGRTYGVEILYRNT
                     DLWGSNITTSFTLFRSEFKDSKEVYIPTAWDNRILFNLTATRPLPKNWDIGFKWRYVG
                     GAPYTPVDLERSSLRQAYDVTGRTYLDYDRFNSERLAPSHQLDVRVDKAFFFNKWSLM
                     LYLDIQNIYNFKSQQPDQYINEDKDGNVVIENPTAPLNEQRYVLRKLENTGSGTVLPS
                     IGIMIEF"
     gene            complement(3807..4841)
                     /locus_tag="PLD76_01160"
     CDS             complement(3807..4841)
                     /locus_tag="PLD76_01160"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOU26648.1"
                     /translation="MKTTLLTLISLLLLASNTFATNIVLNLSNPTTPANINYDTEKKY
                     WTDTYSESSDYTWLQFQNFKLAHLIDQDSWDGTYWDGFTISTSGDNTDYSSTGWTGNE
                     WGCMAGGGIKTDQSGAIVTTNGVVDTEQGIPYLVAFYSAYANNGYSNEIKFADGQKYE
                     AVEVYVTNHPWPYYENMNGGMFTRVLDQEGDYVKLIANGLDENGNSTGSASFYLAKFE
                     NGSLTQVTNWEKFDLSALGTIASLYFTIESTDTGDWGMNTSTFVCMDKLTVKEYTTSI
                     PQLATENAAYRVNNILYNIPENATITVYNLQGQLVYSTMATSNTVSLPIVNNHLIIKI
                     VNENNTQIIR"
     gene            complement(4845..5972)
                     /locus_tag="PLD76_01165"
     CDS             complement(4845..5972)
                     /locus_tag="PLD76_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU26649.1"
                     /translation="MKRNLLVTILLGLIVCNLQAANSAYVTEIIDYRPAPGQHINRSF
                     PPADKSDSHENAEAWAATQLVGKTNGLVGLGAFGGYIIVGFDHSIVNVAEEYDFKVYG
                     NAFANGAEPGIVMVCQDLNGNGVPDNDEPWYELAGSDYQLSSTIKNYEITYTRPNPDL
                     QKSHIQWTDNQGGSGQVTHISFATQSTMYPLWIADNTLTFRGTKLAGNAIQNGSMFTL
                     PALSWGYADNQPNNSTNDKTSFNIDWAVDASGNPVQLSHIDFIKIYTAMVQEAGWLGE
                     TSTEIAGVEDLHPNASLPTVIHSQENKQMYIQNPFNETLIVKANENCEMILYTISGNL
                     IGKYMITPGENTITTSQLPTGNYIVHIINTKQNHIYKLIKH"
     gene            complement(5989..7128)
                     /locus_tag="PLD76_01170"
     CDS             complement(5989..7128)
                     /locus_tag="PLD76_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOU26650.1"
                     /translation="MPIAILSVLIVAFFTACRTDEPVLLPSKTPVTDETTNSEVVGFY
                     LLNEGNMGSNKATLDYFDFTTGIYTKNMYAATNPQVVSELGDVGNDLQIYGNKLYAVL
                     NTSGFVEVMDATTAKHIGMIEIPNCRYITFYNGKAYVSSYAGPVELSNNTQVGYVAEI
                     DTASLQITRTVHVGYQPEEMAIVNGTLYVANSGGYMYPEYDNTVSVICLQTFTETQKV
                     EVGINLHRLKSDNKGNLYISSRGNYTDIASCLYILNTNTLQVDKKVDIPVSNLCIVGD
                     SAYVISSEFNFLSGNTNIEYALINTSTQNIEANNFITDGTETAIKMPYGIAVHPITRD
                     IYITDAKSYIIPGSVYCYSKTGKRKWYVTTGDIPAHIAFLTKQNN"
     gene            complement(7150..>7491)
                     /locus_tag="PLD76_01175"
     CDS             complement(7150..>7491)
                     /locus_tag="PLD76_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU26651.1"
                     /translation="DKTDKTEEYYGHQIPYIPWHSGTAVVAVAYKKWSFNYSFIYTGE
                     RYNASANIPENYVQPWYTHDASISKDFALFGYQWTALVEINNLLNQQYDVVLNYPMPG
                     TNFKCSLKIRI"
BASE COUNT         2086 a         1403 c         1494 g         2508 t
ORIGIN      
        1 aggctggatg tgccaatagt tcgttgagca aagccgaacc aaccaatcct gtgctaccaa
       61 taataagtgc tgttttcata aatgggtgta ttatttgcta tcaaaagtac gcaaaataat
      121 gcaaagtaag catttgatat gcgtgtatat acaaagaaaa agtatctttg caccaaatta
      181 atgtttttac tagtatgaaa aaactccttt gcccacaatg tcgtatccat cggtttcgtg
      241 tttttaatgc agcccgcgag tcggtggtgg taactgtaaa cgaacactat gaggtcatac
      301 ccattcacga gggtgtatcg ctcgaggggt attttactga cgaactccat tgtttgggtt
      361 gctcgtggat aggttcgccc aaagccttac tcaatcggta ttgatacaac aatgagagaa
      421 aaatcgctct tcctctcatt gcatctattg gtttgtgtgt gtgcttaata tacctttact
      481 ttaatcgatt taaatatgtt tttggcagtg gtaatgccag aaatatctgt aaataaggtg
      541 cctgttttac cgtatggatg tccatgacca atcgggttga gtgtgtattc ttgttgcaaa
      601 ttgttcgtat ctatttccac catatacacc aacgatggtt gccccgaaag caggtagtcg
      661 gtattgtttg ggtagcggtt attgtaccac acacggttaa aatcccaggg ttggttaatt
      721 tcaaatacta ttctgtattt tccgtataat tgtctatcta atacgatggt cataacgtcg
      781 gattggcgag gagtagctcc tgtataagca tcgggtatgg gttgttcggg tgttggtaag
      841 tacgtgccgt caggtgcttg tatgtcgcgt ttgtgcaaaa aataaggcag tgttgacggg
      901 cgtcgagctt ctcctttttc tcttttccag cgtttatcgc cagcgtcagc attgttgtag
      961 atgcctgttg ctaaggattg tgtaacgtac aattcttgca caaatttgcc tccagcactt
     1021 tcgagccaca acgcaaaagt agggtagttg taacttttgc ctttttccac actcagttca
     1081 atggttgata gcgtttgctt gtttgctgcc acataagctg tgcttagcag taggctagca
     1141 ataagtagta tatatttttt tgtagtcata cgagttcgtt tttagtgagc tatttagtct
     1201 tgttatataa ttaaaattct atcataattc caatggaagg caatactgtg ccactgcctg
     1261 tattttcgag tttgcgtaat acataccgtt gctcgttgag cggtgccgtt gggttttcga
     1321 ttaccacatt tccatctttg tcttcgttga tgtattggtc gggttgttgg cttttgaaat
     1381 tatagatatt ttgtatatcc aaataaagca tcagcgacca tttgttgaaa aagaacgctt
     1441 tatcaacacg tacatccaat tgatgcgaag gagctaatcg ttcggaatta aatctatcgt
     1501 aatccaaata cgttctgcct gttacatcgt atgcttgtcg caaactagag cgttctaagt
     1561 ctacaggagt ataaggagca cctcccacat aacgccattt aaaccctata tcccagtttt
     1621 tgggcaatgg tctggtggcg gtaagattga acaatatacg attgtcccat gctgtgggga
     1681 tatacacttc tttagagtct ttaaattcgc tacggaataa ggtaaaactg gtggtaatat
     1741 tgcttcccca taaatccgta ttgcgataca gtatttctac cccataggtg cgaccttcgc
     1801 caatagacaa aaccgcttct gctccaaata catcaaaatc ggcacttttg gatgagatag
     1861 ctactgaatc gctgatggag aaaggataat cattgtaaag tttgtaaaaa ccttctactg
     1921 taaaccggct atttgggttt ggtttatttt ctactcccac tacaaaatgg tcggtatatt
     1981 tgtagcgaag acggttatct ttattaatta gattacccgc tatatctctg tatcccatgg
     2041 tggtataagc tggacgttgg tagtagcgtc ctgtattggc atttaacgac catttatttg
     2101 ttaatgcata actagccgaa atacgtggag agaattgttc caatgggttt gacatttgtt
     2161 cgctgtaggt gttgccatct atgcgggctc ctgctgatac gagtaatcgc tcagcgagta
     2221 aattgcggct gatttgtgta aatgccgaat aggaatagaa gtcgagctca gtttcgtaat
     2281 caaactctac tacttgtgca ttgaaaaata atttgttgta ggtataattg gtatattgat
     2341 tgtattcggc accagctcca tacaaaaatt tccacgcacc ttgacgtgtt acatattcgt
     2401 acctaatttt tgtttctatc tcgttcgagg catagtcaaa attgaggtta gtaggtgctt
     2461 cgatgttgtc ttggtatttt tgcgatacat tgttgaggtg gttgcgactc aaaaccaagg
     2521 tttggttacc gtttttaaag aaattgcggt acacagcccc tacggtatat gtccattggt
     2581 cgttggtagg caggtattta agtataaaac gttggctttc tgtttcgttg gcacttaggt
     2641 ttaatctgtt tacatcaaaa gcacctaagc caataaagct tagttcattg ttttggttaa
     2701 aacgaatttt atgcttaaat tgtatatcgt tgtaagtagg caaaaagggc agtccaatca
     2761 ggtcgaaaag aaattctaaa tacgagcgtc ttaccgaaaa tacgaacgaa cttttatcgc
     2821 ctatcggacc atcaagcgta agagccaaat cgctagcacc tactgtggca cgtgttttta
     2881 aacggtcttt attgccatct atcaaccgaa aatcgagtac agaactcaat gcattgcccc
     2941 tgttggcagg aaatgctccc gcaaaaaagt cgagctcgcg tacaaaatct acgttgataa
     3001 tccctgccgg tccgccactt gaaccttgtg tagcaaagtg gttgatgttg ggtatttcta
     3061 cgccatcgag aaaaaaacgg ttttcgttgg caccaccacc acgcacaata atatcgttgc
     3121 ggttggctgg ggttgcacct actcctggaa gagattgaat tactttagaa atatcgcgat
     3181 taccgcctgg gcttttttct atttctttga tgccgatgga gcgtaaactg agtggacttt
     3241 cttcgcgttg cctaaaacga gatgcttcta ccaccacatc atctaactgt atgtttgatg
     3301 gtttcatctc aatttcgata tacggcgtgt tagcgttggt aattagaatt tctttcgtta
     3361 ccacagggtc gaatccaagc gaattaactg ctacccgtac ataaccaggt tttaaaccaa
     3421 agatagcaaa attaccgtct aagtccgtaa tagtaccaat ggtagtgcca tcgataatga
     3481 catacgcaaa atcaaccggt tggttgttgt ctggattgta aaccctacct cgtaatgcac
     3541 ctccttgttc tgcttgtgca aaacctgaaa atcctattgc tgctagtagt gtggcaataa
     3601 ggatggtttt taatgtatat atattcattg taaattgtgc tgagtgattc ttttttttac
     3661 ttaacacaaa agtagaaaat aaatttgata taaaaaatat ttccataaga aaataaattt
     3721 aaaaaaataa gaaaaacctg ttacgtaatt tgatgcaatt atggcagaaa gaatcaaacg
     3781 taacaggttt tagacacagt aaggtattat cggataattt gtgtattgtt ttcgtttaca
     3841 attttaataa ttaaatgatt gttaactatg ggtaagctta cagtatttga tgtagccata
     3901 gtggaataaa caagttgtcc ttgtaagtta tataccgtta ttgttgcgtt ttctggtata
     3961 ttgtatagga tattgttgac tctataggca gcattttcgg ttgctaattg tggtatgctt
     4021 gtagtatatt cttttacagt taatttatcc atgcaaacaa atgtagatgt attcatgccc
     4081 caatcgccag tgtcagttga ttctattgtg aagtacagag atgcaatggt accaagtgca
     4141 gataaatcga atttttccca attggtaact tgcgttaacg agccattttc aaatttagct
     4201 aagtagaaac ttgccgaacc tgtactattt ccattttcgt ctagtccatt ggcaatcagt
     4261 tttacataat caccttcttg gtcgaggaca cgtgtaaaca tacctccatt catattttcg
     4321 taatagggcc aagggtggtt tgtaacatat acttctacag cttcgtattt ttgtccatcg
     4381 gcaaatttta tttcgttact atagccatta ttagcatacg cagagtagaa cgctacaaga
     4441 tatggtattc cttgttctgt gtcaactaca ccatttgtag ttacaatagc tcccgattgg
     4501 tcggttttaa ttcctccgcc tgccatacag ccccattcat ttcctgtcca cccagtggag
     4561 ctatagtctg tattatcacc acttgttgag atagtaaaac cgtcccaata tgttccgtcc
     4621 caagaatctt ggtcgattaa gtgtgctaac ttaaaatttt gaaactgcaa ccatgtatag
     4681 tcagatgatt cgctgtacgt atctgtccag tatttttttt ctgtgtcgta gttaatgttc
     4741 gctggggttg ttgggttgct taggttcaac acgatattgg tggcaaaagt attgctagcc
     4801 aataaaagca aagaaatcag tgtcagtaaa gttgttttca tggtttaatg ttttattagt
     4861 ttgtaaatat ggttttgttt tgtgtttata atatgtacaa tatagttccc agtaggaagt
     4921 tgtgatgtag taattgtatt ttcgcctgga gtaatcatgt attttccgat taaattacca
     4981 gagatggtat ataaaatcat ttcacaattt tcattggctt tcacaatcaa tgtttcatta
     5041 aaaggatttt gaatatacat ttgcttattt tcttgtgaat gtattacagt tggcaatgag
     5101 gcattggggt gcaaatcttc cacgcctgct atttcggttg atgtttcgcc aagccaacca
     5161 gcttcttgta ccattgctgt atagattttg ataaaatcaa tatgggacaa ctgaactgga
     5221 ttgcctgatg catctactgc ccagtcaata ttaaaactag ttttatcatt ggtgctatta
     5281 ttcggttggt tatctgcata accccacgat aaggcaggca aggtaaacat tgaaccattc
     5341 tgaatggcat tgcccgctag tttggttccc ctaaatgtga gcgtattgtc agctatccac
     5401 aatgggtaca tggtactttg tgtggcaaaa gatatgtgtg ttacctgacc gcttccacct
     5461 tgattatcag tccattggat atgcgatttt tgtaaatcag gattgggtcg tgtgtaggta
     5521 atctcatagt ttttaatggt gcttgacagt tgataatcag aaccagccaa ttcgtaccaa
     5581 ggttcatcgt tgtcgggtac tccattgcca ttcaagtctt gacaaaccat cacaatgcct
     5641 ggctccgcac cattagcaaa agcattgcca tatactttaa aatcatattc ttcggctaca
     5701 ttcacaatag aatggtcgaa tccaacaata atatatccgc caaaagctcc cagtccaacc
     5761 aaaccattgg ttttacctac caattgagta gctgcccaag cctccgcatt ttcgtgagaa
     5821 tcactcttat cagcaggagg aaatgaacga ttgatatgtt gcccaggtgc tggacgataa
     5881 tcaataattt cggttacgta agcagaattt gctgcttgta agttacacac aatcaatcct
     5941 aaaagaatcg ttacaagtaa atttcttttc atcattaagt gagttaagtt agttattttg
     6001 tttggttaag aatgcaatat gagctggaat atcacctgtg gtaacgtacc attttcgttt
     6061 gccagttttg ctgtagcaat atacactacc cggaataatg taacttttcg catcagtaat
     6121 atagatatct cgcgtaatag ggtgtactgc tatgccataa ggcattttga tggctgtttc
     6181 tgttccatct gtgataaaat tatttgcctc aatgttttgt gtacttgtgt tgattaaggc
     6241 atactcaatg ttggtattgc ctgacaaaaa attaaattct gaactgataa cataagccga
     6301 atcgcccaca atacataggt ttgaaacagg aatatcaacc tttttatcta cctgcaaggt
     6361 gttggtgtta aggatataca agcacgaagc aatgtcggtg tagttacctc tactactgat
     6421 atataggttt cctttattgt ctgattttaa tcgatgaaga ttaatgccta cttcaacttt
     6481 ttgtgtttcg gtaaaagttt gtaggcaaat tacagaaacc gtattatcat actctggata
     6541 catatatccg ccagaattag caacatacag cgtaccatta actatagcca tttcttcggg
     6601 ttggtaacca acatgtactg ttcgggttat ttgcaaactt gctgtatcaa tctcagctac
     6661 ataacctact tgcgtattgt tacttaactc aacaggtcca gcatacgaac ttacataagc
     6721 ttttccatta taaaatgtga tataacggca attgggtatt tctatcatgc ctatatgttt
     6781 ggcagtagtg gcatccatta cttctacaaa tccagaggtg ttgagaacag cgtataattt
     6841 gtttccataa atttgcaaat cattgccaac atcacccaat tctgatacta cctgagggtt
     6901 ggtagccgca tacatatttt tagtatatat gcctgttgta aagtcgaaat aatcgagcgt
     6961 agctttgttg ctccccatat taccttcgtt taacaaataa aaacccacta cctcgctatt
     7021 agttgtttcg tcggttacag gcgttttact tggcaaaagt acaggctcat ctgtgcgaca
     7081 agctgtaaaa aatgccacta ttagcacaga gagaatagct attggtatgt aataattttt
     7141 tttgttcatt tatatacgta tttttaagct acatttaaaa ttcgttcccg gcattgggta
     7201 gtttagtacc acatcatatt gttggtttag taggttattg atctccacta aagcagtcca
     7261 ttgataacca aataatgcaa aatctttcga aatgcttgca tcatgtgtgt accaaggttg
     7321 tacatagttt tcgggtatat tggcactggc attgtatcgt tcgccagtgt agataaagct
     7381 ataattgaac gaccattttt tgtatgcaac agctactact gcggtgccgc tatgccaagg
     7441 gatataaggt atttgatgac cataatactc ttctgtttta tccgttttgt c
//
LOCUS       DAOSMY010000026         7267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_10284_length_7267_cov_3.081392, whole genome shotgun sequence.
ACCESSION   DAOSMY010000026 DAOSMY010000000
VERSION     DAOSMY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10284_length_7267_cov_3.081392"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLD76_01180"
     CDS             <1..301
                     /locus_tag="PLD76_01180"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOU26652.1"
                     /translation="IDYDNHSELALKYTFDTSAITYLEEKIDTYTQLIPPLKGFVIPG
                     ETLSSAYCDACRTICRRAERRIYDITLHPHQQNAAIFINRLSDFLYVLARKLTKL"
     gene            402..623
                     /locus_tag="PLD76_01185"
     CDS             402..623
                     /locus_tag="PLD76_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOU26653.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPLEVIENLQEME
                     DEGEIYETIEDIWPDYPSKEDFFFNEDEY"
     gene            737..1681
                     /locus_tag="PLD76_01190"
     CDS             737..1681
                     /locus_tag="PLD76_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU26654.1"
                     /translation="MKKKLLVWLFFVATTINGIAQQNMQFSLYTLNLNAINPASTGIN
                     EMIHISGVHRQQWLNIKRAPQITYMNIELPFSIENSKHGAGIGFLNQGIGLFTNQSIV
                     LKYAYHKKINEKTNLSGGLNIQFNSIGFNIKDATTGTSDFHSPNDPAIPTGSEISDLK
                     TDIGVGAMVKNETYLLGVALQNIVSPTYQLSNTEYTTPMNINLVGIYNIKTISSLYLI
                     QPSVLVKTDFATWQCELGSTIQYKEKYWGGLGYRFQDAIIFQVGMQLANGLTIGYAFD
                     LPISSMIYTSVGSHELSLSYSFKIEFSKKQTYKSIRHL"
     gene            1702..3114
                     /locus_tag="PLD76_01195"
     CDS             1702..3114
                     /locus_tag="PLD76_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU26655.1"
                     /translation="MKKLLILFAALAALVGCSNKGSGELVGVQGDAWKDPTPYGMVFI
                     PSGSFTMGPNDQDANWALSSQSKVVSVDAFWMDETEITNREYKQFVYWVRDSIARTKL
                     GEVDEAFLVTQDKRGNELEKPRINWKPKIPWSKANEEQAEAIDAMFYADDELIALTKE
                     LNGHILHYKYAWIDYNQAALKRNKFNTNTGKFEADLSLLGKGKDSADVMVKKDTAYLT
                     EEGYIVNQTKFVELKNRRDFISSKIINVYPDTLSWVRDFTYAYNEPYMKMYFYHDGYG
                     DYPVVGINWEQANAFCHWRTNFYNKAQIDNKRHIVQDYRLPTESEWEYAARGGRDLSM
                     YPWGGNYIRTAKGCYMANFKPQRGNYTVDGHMVAAKVASFPPNNYGLYDMSGNVAEWT
                     SSAFHESNYSFVNDLNPSYQYNAKASDPEIMKRKVIRGGSWKDIGFYLQCGTRTYEYQ
                     TESRSYIGFRCVRSVIGANY"
     gene            3160..3978
                     /gene="gldL"
                     /locus_tag="PLD76_01200"
     CDS             3160..3978
                     /gene="gldL"
                     /locus_tag="PLD76_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOU26656.1"
                     /translation="MSKGSFLSSPQGKKMTAKAYGFGAAIVIIGALFKIMHFPGAGIM
                     LSVGMGVEALLFALSAFEDPHKEYDWSIVYPELDGGEAEERAPKVKAGAPLSVSGVNP
                     LPQDDVEKLKEGIRKISETASQFADISQASAASSNYVKNMNVASEAISAFASTQVGLK
                     NSSDSLFQSYRTVAESMTAAASDAKGYMQEMQGITRNLSSINASYELQLKNIGSQAQM
                     LEGMTTDMGKIKTAMNDAANETEAFKAQATKLTQQISSLNNVYGNMLNAFNARV"
     gene            4025..5578
                     /gene="gldM"
                     /locus_tag="PLD76_01205"
     CDS             4025..5578
                     /gene="gldM"
                     /locus_tag="PLD76_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOU26657.1"
                     /translation="MGATNCPETPRQKMIGMMYLVLTAMLALNVSADVLNGFTLVHAS
                     LNESKSTTEMQNTILYGQFEDLKSQNPGRVGEWLEKANAVKAKTAEMYQMIEDLKVDI
                     VKQADGADGDVNNIKSKSNLDAGGQVALVTGRAKKMKEAIELYKDEMLKYVGKDSAKA
                     NYLNRTYNTGMITKENGDKISWEGAIFESMPVVASITLLTKVQADLKHTELEVVNYLK
                     NQIDAGDFRVNKIEGLVISESNYVTRGGRYSAKIVLAAQDSTKKPTITVNGRELDGNE
                     YIVQAGSVGTFPVKGTIRLPRPDGSTAEYDFESSYIVGEPTAIISADMMNVLYAGIQN
                     PISVSVPGIPAQNINVSVNNGALTRTAKGWNINPRVGQDCNISVSAKMADGRVQTIGS
                     KPFRVKPLPPPIAYIEFRDDKGNLQQYKGTKPFPKAQLVGSNGIRAALDDADLDVRYQ
                     VLGFDVNFFDSMGNTIVETSNNNSFSDKQMATMRQMPRGRKFFISRVRAKGPDGIERI
                     LPPIEVIVN"
     gene            5591..6481
                     /gene="gldN"
                     /locus_tag="PLD76_01210"
     CDS             5591..6481
                     /gene="gldN"
                     /locus_tag="PLD76_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOU26658.1"
                     /translation="MKKTFYQLFFAGLFLMFGNIAVAQEEEGGVSFFGENGELATNIP
                     TSVSLEERKPIEYNNPRADDIIWQKVVYRIVDLREKMNFPLYFPEETSDNRQSLFTTI
                     FRLFERDQIKCYSYKDNVEFFTENELVKFDDIFSKYGMVRPVKRDSVSGDTTIVEFDE
                     SDIPNREILKYYVKEVWYFDKHNSHFNVRIMAICPIWYRQDYDLGLQKYPLFWVPFDL
                     LRPYLAQNEILVSDRNNGARETMDDLFIKRRFGSYIYKESSTRNRNLLQYNSTAEQMN
                     REQERIKNEIFNFEQDLWEY"
     gene            6679..>7267
                     /locus_tag="PLD76_01215"
     CDS             6679..>7267
                     /locus_tag="PLD76_01215"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOU26659.1"
                     /translation="MKKMKGIKKCVLFGVFVSLYSIANAQYNHEIGITGGGSFYLGDA
                     NLSLPFHKTNVAYGGFYVYNIDTRYALKAHVIAATIEGDSRDFNYIFPNQQFGYFDRK
                     LIDIGLNLQFNFFDLGQSKYYNSHNSITPYFMIGMGYLAYNNIYIGDNVYKFTIPMGI
                     GGKWKMNDRFTLGIEWKINKLFIDDLAQLAKSWNFI"
BASE COUNT         2595 a         1146 c         1363 g         2163 t
ORIGIN      
        1 tattgactac gacaatcata gtgaattagc cctgaagtac actttcgata ccagtgcaat
       61 aacctattta gaagaaaaaa ttgatacata cacgcaatta atacccccat taaaagggtt
      121 tgttatacca ggagaaacac ttagctctgc atattgcgat gcatgtagaa caatctgtag
      181 acgtgccgaa agaagaatat atgacattac tctacacccc catcaacaaa atgcagccat
      241 atttatcaac agactgtctg attttttata tgtactcgca cgaaaattaa caaaattgta
      301 atttaaaaaa ttgtaaagaa agtattgcaa actaaaaaaa tatttttacc tttgcgaaca
      361 gttttagtgg attttttaat aaaacttaat aatcgaatga tatgtattgg acgttagagt
      421 tagcatcaaa attagaagat gcaccctggc cagccaccaa agatgagttg atagattacg
      481 cgattaggtc tggagctcct ttagaggtaa ttgaaaatct gcaagagatg gaggatgaag
      541 gtgaaatata tgaaacaata gaagatattt ggcctgatta tccaagtaaa gaagatttct
      601 tctttaacga agatgaatat tgattttttc tgcatttttg ttgttttaca caataaattt
      661 gcaaaaaatc tgttaataaa tagcaagcgg caatttatcc tagtggttaa gatttagctt
      721 agccgccata ttatttatga aaaaaaaact tctcgtatgg ttgttttttg ttgctaccac
      781 tatcaatggt attgcccaac aaaatatgca atttagtctt tacacactaa atttgaatgc
      841 cattaatcca gcatctactg gaataaacga gatgatacat atatctggag tacaccgtca
      901 acagtggttg aacatcaaaa gagcacctca aattacgtat atgaatattg agttaccctt
      961 ttcgattgaa aactccaaac atggtgctgg tatcggattt ttaaatcaag gaattggtct
     1021 ttttacaaac cagtctattg tcttaaagta tgcatatcac aaaaaaataa atgaaaaaac
     1081 aaacctcagt ggaggattga atatacaatt taatagtatt gggtttaata taaaagatgc
     1141 aacgacagga acaagtgatt ttcattcgcc aaatgatcct gccattccaa caggttccga
     1201 aatatctgat ttaaaaacag atatcggcgt tggagccatg gtcaaaaatg aaacatactt
     1261 gcttggcgta gcactacaga atattgtatc tccaacatat caattgagca acacagagta
     1321 tactactcca atgaacataa atttagttgg tatatacaat ataaagacaa taagttcgtt
     1381 atacctaata caaccatctg ttttagtgaa aactgatttt gcaacatggc aatgtgaact
     1441 agggtcaacc atacaataca aagaaaaata ctggggcgga ttaggataca gatttcaaga
     1501 tgctattatt tttcaagtag gtatgcaatt ggcaaatgga ctaacaatag gatatgcatt
     1561 tgatttacca atttcatcca tgatatatac tagtgttgga tctcatgaat tgtcattatc
     1621 atatagtttt aaaattgaat tttcgaaaaa acagacatac aaaagcattc gacatttata
     1681 agcttaacct aaaaattaga tatgaaaaaa ttacttattt tatttgccgc tcttgcagcc
     1741 cttgttggtt gcagtaacaa aggaagtgga gaattagttg gtgtacaagg tgatgcttgg
     1801 aaagatccca ccccttatgg tatggtgttt atcccatctg gatcctttac catgggaccc
     1861 aacgatcaag atgcgaactg ggctctttct tctcaatcta aagtggtctc agtagatgct
     1921 ttttggatgg atgaaaccga aatcacaaac cgtgagtaca aacaatttgt gtattgggta
     1981 cgtgactcaa tcgcacgtac taaacttggt gaagtagatg aagctttcct tgttacacaa
     2041 gacaaaagag gaaatgaact agaaaaacct agaattaact ggaaaccaaa aattccatgg
     2101 tcaaaagcaa atgaagaaca agcagaagct atagatgcaa tgttttatgc tgatgacgaa
     2161 ttaattgctc ttacaaaaga attgaatgga catatcctcc attacaaata tgcatggatt
     2221 gactataatc aagcagcctt aaaacgcaac aagtttaaca caaatacagg taaatttgaa
     2281 gcagatttat ccttactagg taaaggaaaa gatagtgctg atgtaatggt aaaaaaagat
     2341 actgcctatc ttacagaaga aggttatatc gtaaatcaaa caaaatttgt tgaattaaaa
     2401 aacagaagag attttatttc ttctaaaata attaatgttt atccagatac tctttcttgg
     2461 gtacgcgact ttacctatgc atacaacgag ccatacatga aaatgtattt ctatcacgac
     2521 ggttatggag actatcctgt tgtaggcatc aactgggagc aagcaaatgc tttctgccat
     2581 tggagaacaa atttctacaa taaagcacaa atagacaaca aacgtcatat tgtacaagac
     2641 taccgcctcc ctactgagtc tgaatgggaa tacgctgctc gtggtggtcg cgatttatcc
     2701 atgtacccat ggggtggaaa ttatatccgt actgcaaaag gttgctatat ggcaaacttt
     2761 aaacctcaac gaggtaatta tacagttgat ggacatatgg tcgctgctaa agtagcttct
     2821 ttccctccta ataattacgg attgtatgat atgtcaggca acgttgcaga atggacttcg
     2881 tctgcgtttc acgaatctaa ttatagtttt gtgaacgact tgaatccaag ttatcaatac
     2941 aatgccaaag caagcgatcc tgaaatcatg aaacgtaaag ttattcgtgg tggctcatgg
     3001 aaagacatag gattctattt acaatgtggc actagaactt atgaatacca aacagaatca
     3061 cgttcgtata ttggatttcg ttgtgtaaga agtgtaatcg gagcaaatta ctaaataaat
     3121 taataataaa ttatctatta acctaaaaat cacacaaaaa tgtctaaagg aagtttctta
     3181 agtagccctc aagggaaaaa aatgacggca aaggcgtatg gctttggtgc tgcaatagtt
     3241 atcatcggtg ctttatttaa aattatgcac ttccccggtg ctggtattat gttatcagtc
     3301 ggtatgggtg ttgaggcatt actatttgca ctgtctgcat ttgaagatcc acacaaagaa
     3361 tatgattgga gtatcgttta tccagaatta gatggtggag aagcagaaga aagagctcca
     3421 aaagtaaaag ctggtgcacc attaagcgta tctggagtta atccattacc acaagatgat
     3481 gtagaaaaat taaaagaagg tatccgtaaa ataagtgaaa cagcttctca atttgcagat
     3541 atttcacaag caagtgcagc ttcatctaat tatgttaaaa acatgaacgt tgcttctgaa
     3601 gctatcagtg cttttgcttc tactcaagta ggtcttaaaa actcttctga ttcattgttc
     3661 caatcatata gaacagtagc agaaagtatg actgcagcag cttctgatgc taaaggttat
     3721 atgcaagaaa tgcaaggcat cacacgtaat ctttcttcta tcaatgctag ctacgaactg
     3781 caattgaaaa acattggatc acaagctcaa atgttagaag gaatgactac tgatatgggt
     3841 aaaattaaaa cagcaatgaa tgatgctgct aacgaaactg aagcatttaa agcacaagct
     3901 accaaattaa cacaacaaat tagcagcttg aacaacgtat atggtaatat gcttaatgcg
     3961 tttaacgcaa gagtgtaatt aacaagaatt gtttaaccta atttaaaata aataataaaa
     4021 taatatgggt gcaactaatt gtccggaaac cccgagacag aaaatgatag gtatgatgta
     4081 cctggtactc acggcaatgc ttgcattgaa cgtatctgct gacgtactga atgggtttac
     4141 cttggttcat gcaagcttga acgaaagtaa atcaacaact gaaatgcaaa acacaatcct
     4201 ttatggacaa tttgaagatt taaaatctca aaatcctgga cgtgttggcg aatggcttga
     4261 aaaagcaaat gcagtaaaag caaaaacagc cgaaatgtat cagatgatcg aagacttaaa
     4321 agttgatatc gtaaaacaag ctgatggtgc tgacggtgat gtgaataaca ttaaaagcaa
     4381 atctaacctt gatgctggtg gtcaagtggc tcttgttaca ggtagagcta aaaaaatgaa
     4441 agaggcgatt gaactttaca aagatgaaat gcttaaatat gtaggtaaag attcggcaaa
     4501 ggcaaactac ttaaatcgca cttataatac aggaatgatt actaaagaaa atggagataa
     4561 aatatcttgg gaaggagcca tttttgagtc aatgcctgta gttgcttcta taactttact
     4621 cacaaaagtg caagcagacc taaaacacac tgaattagaa gtagtaaatt acctaaaaaa
     4681 ccaaattgat gcaggagact tccgtgtaaa taaaattgaa ggacttgtaa tttcagaatc
     4741 taattatgta actagaggtg gtcgttactc agctaaaatt gtattagctg cacaagactc
     4801 tactaaaaaa cctaccatta ctgtaaatgg aagagaatta gatggcaatg aatatattgt
     4861 gcaagcgggt tctgttggaa ctttcccagt aaaaggaact atacgtttac ctcgtcctga
     4921 tggaagtact gcggaatatg attttgaaag ttcgtatata gtgggcgaac ctactgcaat
     4981 tatttcggca gatatgatga acgtactata tgctggtatt caaaatccaa ttagtgtttc
     5041 tgtaccaggt attcctgctc aaaacatcaa tgtaagcgtt aacaatggag ctcttactcg
     5101 cacagcaaaa ggatggaata tcaatcctcg tgtgggtcaa gattgcaata tctctgtttc
     5161 tgcaaaaatg gctgatggac gtgtacaaac aatcggttct aaaccgttta gagtaaaacc
     5221 tcttccacct ccaatagcat atatcgaatt tagagatgat aaaggaaatc tacaacaata
     5281 taaagggact aaaccttttc caaaagcaca attggttgga tcaaatggaa ttagggcagc
     5341 tttagatgat gctgacttag atgtacgtta tcaagtgtta ggttttgatg ttaacttctt
     5401 cgattcaatg ggtaatacta ttgtagaaac aagtaacaac aattctttct ctgacaagca
     5461 aatggcaact atgagacaaa tgcctagagg tagaaaattc tttatttcac gtgttcgtgc
     5521 taagggtcct gatggtattg aacgcatatt gcctccaatt gaagttattg taaattaaaa
     5581 tttcactgaa atgaaaaaaa cgttttatca actattcttc gcagggttat tcttgatgtt
     5641 tggcaatata gctgtagcac aagaagaaga aggaggagtt tctttctttg gcgaaaatgg
     5701 agaacttgct accaatattc caacctcagt atcactggag gaaagaaaac caattgaata
     5761 caacaaccca agggcagatg atattatttg gcaaaaggtg gtgtatcgca ttgttgacct
     5821 tagagaaaag atgaatttcc cactttactt ccctgaagaa acaagtgata atcgtcaaag
     5881 tttattcact accattttta gattatttga aagagatcaa attaaatgtt attcatacaa
     5941 agacaatgtt gaatttttta ctgaaaatga gcttgtcaaa tttgatgaca ttttttctaa
     6001 gtatggaatg gtgagacctg taaaaaggga ttcagtatct ggtgatacaa caattgttga
     6061 gtttgatgaa agtgatattc ctaatagaga gattttaaaa tattacgtaa aagaagtgtg
     6121 gtattttgat aaacacaatt ctcactttaa cgtacgtatt atggcaatct gtccgatttg
     6181 gtatcgtcaa gattatgatt taggattgca gaaatatcct ttattttggg taccttttga
     6241 tctgctaaga ccttatttgg ctcaaaatga aatattggta tcagatagaa ataatggtgc
     6301 tcgcgaaacg atggatgatt tatttatcaa acgaaggttt ggtagttata tctataaaga
     6361 atcaagtaca cgtaatcgaa acttattgca atataatagt acggcagaac aaatgaatcg
     6421 agagcaagaa cgtatcaaaa acgagatatt caattttgaa caagatttgt gggaatacta
     6481 atcacacgta aatcataaaa acagaaaagt cgctgcatca tcagcgactt ttctgtttta
     6541 cttatatttt gttaaatata atggaataat gagagtgttt gaccgattaa taagaagata
     6601 ttcagtatat ttgttggctc aatatcaact aaaaaaagag attatatcga ttataagaat
     6661 acgtactaag tgaaaagtat gaaaaaaatg aaaggcataa aaaaatgtgt cctgtttggt
     6721 gtattcgttt ccttatattc gatagcaaac gcacagtata atcatgaaat tgggattaca
     6781 ggaggaggct ctttctattt gggagatgcc aatctttctc tcccttttca taaaacgaat
     6841 gtagcgtatg gtgggtttta tgtctataat attgacacac gctatgcgtt gaaagctcac
     6901 gttatagcag ctacaataga aggagatagt agggatttta actacatctt tccaaaccaa
     6961 cagtttggat attttgatag aaaacttatt gatattggct taaatttgca atttaatttt
     7021 tttgatttag gtcaatcgaa atattataat agtcataatt caattacccc ctactttatg
     7081 ataggcatgg gatatttggc gtataacaac atctatatag gagataatgt atataaattt
     7141 actattccaa tgggtattgg aggaaaatgg aaaatgaatg accgatttac gttggggata
     7201 gaatggaaaa tcaataaatt atttattgat gatttagcac aattggcgaa aagttggaat
     7261 ttcatat
//
LOCUS       DAOSMY010000027         7131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_10542_length_7131_cov_3.246326, whole genome shotgun sequence.
ACCESSION   DAOSMY010000027 DAOSMY010000000
VERSION     DAOSMY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10542_length_7131_cov_3.246326"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLD76_01220"
     CDS             <1..811
                     /locus_tag="PLD76_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOU26660.1"
                     /translation="VFHTAFNIINTFIMIFFVGLIAKTVSWIIPKKKSDEEFHLTHIS
                     TGLLSTSELSIIQATKEVNVYAERTLRMFYMVKELYVEKDVQAFQKTYDRIAKYENIS
                     DRMEVEIAQYLTKVAEGRLSDESKNRLQSILRMVSEIESIGDSCYNMARIIKRRNEDK
                     SEYTEQMNANVEKMFDSVIKAYDEMMHVLADFDTKTSVDLGYCKEIESEINELRNALK
                     MQNVVDVKGKKYSYQASVTYMDLIIECEKLGDYIINVEEAVKDSTTLTKKI"
     gene            complement(816..1460)
                     /locus_tag="PLD76_01225"
     CDS             complement(816..1460)
                     /locus_tag="PLD76_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU26661.1"
                     /translation="MYYNIHSHKVAKCDNTITIYNVSLHNDITISALSSIGIAPWESD
                     LYTKTNVALLTKYGINENVVAIGEVGLDKRIDTPLTQQLAIFTHQILIAEQCNKPMII
                     HCVGYYNELIQLFRKRRPTVKYILHGFRGKSPLAQELVKHGFYISLGEKSVLHQESIQ
                     QIPLNKILIETDDSDIHVEEVYKSISSIKNISVEELKKSIEENITQCFGWNLRN"
     gene            complement(1477..1698)
                     /gene="yidD"
                     /locus_tag="PLD76_01230"
     CDS             complement(1477..1698)
                     /gene="yidD"
                     /locus_tag="PLD76_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU26662.1"
                     /translation="MKKLLQTILLIPIYIYRGIISPIKPPSCRYTPTCSEYAIQAIKK
                     HGPLKGMHLAIRRLLSCHPWGGSGYDPVP"
     gene            complement(1698..2105)
                     /gene="rnpA"
                     /locus_tag="PLD76_01235"
     CDS             complement(1698..2105)
                     /gene="rnpA"
                     /locus_tag="PLD76_01235"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU26663.1"
                     /translation="MKTLRFTFPKKEHLCGKTTIDKLFEKNNSFLQYPLRVLYKIEKN
                     PDNKIPAKILLSVSKKYFKRAIHRNTLKRKMREAYRKQKSPLILLLSEKNLSITMAIL
                     YISKEPISYEQIDAGMKECITKLMHKIENTTPK"
     gene            complement(2182..2943)
                     /locus_tag="PLD76_01240"
     CDS             complement(2182..2943)
                     /locus_tag="PLD76_01240"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOU26664.1"
                     /translation="MKIKKILVSQPAPENDKSPYYDIAEKYNVKIEFRPFIKVEPLPL
                     KDFRQQRISILEHTAVIFTARTAIDHFFSICEELRITVPETMKYFCVTETIANYLQKF
                     IDYRKRKVFFPKTGKQEDIIIPVTKHKTEKFLLVVSDVHTDKFVSLLEKNTIQYTKGV
                     FYRTVSNYFSPEEAFDYDMLLFFSPAGVDSLFKNFPNYQQGEVYIGCFGTTTALAVKN
                     AGLRLDVEAPTKESPSMTSALDAFLKEKAKMNGKK"
     gene            complement(3798..4382)
                     /locus_tag="PLD76_01245"
     CDS             complement(3798..4382)
                     /locus_tag="PLD76_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU26665.1"
                     /translation="MSLSVCIYCASSNAINSVYYQETEKLARLLAQNNVNVKFGGGSS
                     GLMGKIADVYVEENNDNIVGIIPKFMCEEGWQHQQLKQLIVTETMHERKQKMIENVDA
                     AIALPGGIGTLEEVLEVLTWKQLGIFLKPILLVNINGYFDPLLIALEHAVSENFMRKE
                     HLQLFTIISSVENILQDIQKNPTYDVSIRKIAQI"
     gene            complement(4379..4927)
                     /locus_tag="PLD76_01250"
     CDS             complement(4379..4927)
                     /locus_tag="PLD76_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU26666.1"
                     /translation="MQKRKITELNRISVEEFQSKTKLPLVVILDNIRSLHNIGAIFRT
                     CDAFLIEEIYLCGITATPPNAEIHKTALGAENTVKWVYFESIMDAIRQLQTKGYSVFA
                     VEQVKQSIPLNELKIDVTKKYAIIVGNEVKGVQQVAVDMCNGAIEIPQFGTKHSLNVS
                     IAAGIVIWDLVKELSTSQSERG"
     gene            5075..5851
                     /gene="argB"
                     /locus_tag="PLD76_01255"
     CDS             5075..5851
                     /gene="argB"
                     /locus_tag="PLD76_01255"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOU26667.1"
                     /translation="MKQSLIIIKVGGKIVEESVSLASLLKDFAHTEGYKVLVHGGGRS
                     ATKLSEKLGIESKMVDGRRITDKQTLDVVTMVYGGLVNKNIVAGLQALGVNALGLTGA
                     DMNIIESVKRPIKEVDYGYVGDVKHVNAQIVASLIQQGIVPIVAPLTHDGAGSMLNTN
                     ADTIASELAKSLSTYFDVTLVYCFEKNGVLSDEMDDNSVIPLITPSLFNKYVHQGIIK
                     GGMIPKLENAFQAIQAGVSKVVITNATAIGKDAGTVVLTD"
     gene            5856..6876
                     /locus_tag="PLD76_01260"
                     /pseudo
     CDS             5856..6876
                     /locus_tag="PLD76_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(6862..>7131)
                     /locus_tag="PLD76_01265"
     CDS             complement(6862..>7131)
                     /locus_tag="PLD76_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU26668.1"
                     /translation="FKNENVKVITYTNLTADIVKAEKASCIVRGIRGISDYEYEKNMA
                     DINKELFEIETVFLFSDLKYAHISSSLIRELKRFGKEYKHLLPES"
BASE COUNT         2310 a         1084 c         1280 g         2457 t
ORIGIN      
        1 tgtgtttcat actgcattta atattattaa tacttttatt atgatatttt ttgtaggtct
       61 tattgctaaa acggtatctt ggataattcc taaaaagaaa tcagacgaag aattccatct
      121 tacacatata tctacaggtt tgctttctac ttccgaattg tctattattc aagcaactaa
      181 agaggtaaat gtgtatgccg aacggacgtt gcgtatgttt tatatggtga aagaattgta
      241 tgtagaaaaa gatgtgcagg cttttcaaaa aacctatgat agaattgcta agtatgaaaa
      301 tattagcgat agaatggagg ttgagattgc tcaatattta accaaggtgg cagaaggcag
      361 attgagtgat gagagtaaaa accgtttgca aagtatttta cgtatggtgt ctgaaataga
      421 aagcattgga gatagttgtt ataatatggc acgtattata aaacgcagaa atgaagataa
      481 atcggagtat actgagcaaa tgaatgcgaa tgtagaaaag atgtttgatt ctgttattaa
      541 agcgtatgat gagatgatgc acgtgcttgc tgattttgat acaaaaacat ctgttgattt
      601 gggctattgt aaagagattg aatcagaaat aaatgaatta agaaatgccc tgaaaatgca
      661 aaatgttgtg gatgtaaaag ggaaaaaata ttcatatcaa gcgagtgtaa cgtatatgga
      721 tttgattata gaatgtgaaa aattgggtga ttatattatc aatgttgaag aagcggtaaa
      781 agactccaca acattaacaa agaaaatata atgtcttaat ttcgcaaatt ccatccaaag
      841 cattgagtga tattttcctc aatgcttttt tttagctctt ctactgaaat gttttttata
      901 ctagaaatag atttgtaaac ctcttctaca tgtatgtcag aatcatctgt ttctatcaat
      961 attttgttca gaggtatttg ttgaatagac tcttggtgaa gtacgctttt ttctcccaat
     1021 gaaatgtaaa atccatgttt aacaagttct tgtgctaatg gagattttcc tctaaatcca
     1081 tgtaggatat atttaactgt aggtcggcgt ttccgaaata attgtattaa ttcgttatag
     1141 taacctacac agtgtattat cattggttta ttgcattgct ctgctattaa aatttggtgt
     1201 gtgaagatag ctaattgttg tgttaaaggt gtgtctattc gtttatctaa acccacttcc
     1261 ccaatggcaa ccacgttttc gtttatacca tatttagtta acaatgctac atttgtctta
     1321 gtgtataaat cgctttccca aggagcaata ccaatagatg acaatgcgga gatagtaata
     1381 tcattatgta gagaaacatt atatatggta atggtattat cgcattttgc taccttatgg
     1441 gagtggatat tgtaatacat tttcttcgta ttagaattat ggaacaggat catatccgct
     1501 acctccccac gggtgacagc ttaataaacg tctaattgct aaatgcattc ctttcaaagg
     1561 tccatgtttt tttattgctt gtatcgcata ttcagagcag gtaggggtat acctacatga
     1621 cggaggtttg ataggagaaa tgatgcctct gtagatgtat atcggtatta gcagtatggt
     1681 ttgtaatagt ttttttatca ttttggtgtt gtattctcaa ttttgtgcat gagttttgtg
     1741 atacactctt tcatccctgc atctatttgc tcatagctta taggttcttt agaaatgtat
     1801 agaatagcca ttgtaataga tagatttttt tcagataaaa gcagtattaa agggcttttt
     1861 tgtttgcgat aagcttctcg catttttctt tttagtgtat ttctatgtat tgctctttta
     1921 aaatactttt tggatactga tagtaagatt tttgcaggaa tcttattatc gggattcttt
     1981 tctattttgt ataatacacg taaaggatac tgcaaaaatg aattgttctt ttcgaacaat
     2041 ttatcaatgg tcgtttttcc acacaagtgt tcttttttag gaaaggtaaa acgaagtgtt
     2101 ttcatactaa aaaaatatct ctcaaagata gctttttttt tcaagctaat caatgagaga
     2161 tactattact tcaaaatggt gttatttttt tccattcatt tttgcttttt cttttaagaa
     2221 agcgtccaaa gcacttgtca ttgagggaga ttcttttgta ggagcttcta catctaatcg
     2281 taatcctgca ttttttacag ctagggctgt ggttgtgcca aaacatccaa tatacacttc
     2341 tccttgttga taattaggga aatttttgaa caaagagtca actcctgcag gactaaaaaa
     2401 tagcaacata tcatagtcaa aagcctcttc gggcgaaaaa taattgctca ccgtacgata
     2461 gaatactcct tttgtgtact gaattgtatt tttttctaat agagacacaa atttatcagt
     2521 atgtacatca gatacaacta acaaaaactt ttccgtttta tgttttgtaa cgggtattat
     2581 aatgtcttct tgttttcctg tttttggaaa aaaaacttta cgtttacggt aatcaataaa
     2641 tttttgtagg taatttgcta ttgtttcggt tacgcaaaaa tatttcattg tttcggggac
     2701 agtaatacgc agttcttcac atatcgaaaa aaaatggtcg atagcagtgc gagcagtaaa
     2761 aataacagca gtatgttcta aaatagatat gcgctgttgt cggaaatctt ttaaaggcag
     2821 aggttccact ttaatgaatg gacgaaattc tatctttaca ttgtattttt ctgctatatc
     2881 ataatacgga gatttatcgt tttcaggagc tggctgagat acgagtattt tcttgatttt
     2941 caatgcttta caatttaatg gttgataaaa tatatgtccc ctacaaaaaa ctttataagt
     3001 aaacataagg gtaaaatttc cagagtgcaa aggtacaaaa taaaatataa aactaaatgt
     3061 attttactga aaaaagttct gtatatgata taaaacattg acaataacgt taataaggct
     3121 agtattgcta ccccgtaata taggtatatg tgtacgtatt cattactaaa taaaattccc
     3181 aaaagaaaca gaaaggtaag ttgtccacta tagaaaatga ttgaaaaata ttgccttata
     3241 gcgagactgt ttgttgttga aaaaaatgta tacacataga gtttcaacaa gatatatttg
     3301 ataaaaaaat acagaaaaag ggcaacaaaa agccatcctc ctatttccca tgtttcataa
     3361 tagtttgtga tagtagattt ttctacaact aaataaagtg ctatgctcgt gtatccaatt
     3421 aatccaaata gactatacag aacttgtttc cattgttcta atactgttag attaatttgt
     3481 tccgtttcgt ttttgtgttt aaatatactt gtaaaataat gtgttttggc tttacgaaaa
     3541 aatgcaatag taatacacag taaaataaat atgctcaata gtaatgcaat cacactatca
     3601 gaatgaaaaa atgaagttgt tcgccatata ctttccattc cttcgggaat cagtatccgc
     3661 gtactatcta tgtgcatagt agaagaaaag agtatgtcat cccacactgg aacatggctt
     3721 gtaatagagt cagggctctg caacaaaggg ggatagttat ttgtaatctg tactaaagag
     3781 tcttctggca tagatagtta aatttgtgca atttttctta tggatacatc atatgtaggg
     3841 tttttttgta tgtcctgcaa tatgttttct accgaactta tgatggtaaa taattgtaaa
     3901 tgttcttttc tcataaagtt ttctgacaca gcgtgttcta acgcaataag tagtggatcg
     3961 aaatatccat taatgtttac gagtaagata ggttttaaga aaattcctaa ttgtttccaa
     4021 gtgagtacct ctaacacttc ttctaacgta ccaatcccac ccggtaaagc aattgctgca
     4081 tctacatttt ctatcatttt ttgttttctc tcgtgcatag tttctgttac aattaactgt
     4141 tttagttgtt gatgttgcca gccttcttca cacatgaatt tagggataat tcctactata
     4201 ttgtcattgt tctcttccac atacacatct gcaatttttc ccataagtcc gctactacca
     4261 cctccaaact taacgttaac gttgttttga gccaataatc tagctagttt ttctgtctct
     4321 tgataataaa cagagtttat ggcattgcta gatgcacaat atatacatac cgataagctc
     4381 atcctctttc tgattgtgat gtagataatt cttttaccaa atcccaaata actattcctg
     4441 ctgcaatgga tacgtttagt gagtgctttg ttccaaactg tggtatttca atagcaccgt
     4501 tgcacatatc tacagctact tgttgcactc cctttacttc gttgcctaca attatagcgt
     4561 atttttttgt tacgtcaatt ttcaattcgt tgagaggaat gctttgtttt acttgctcta
     4621 cggcaaatac ggagtatccc ttggtctgta attgtcggat tgcatccatt attgattcaa
     4681 aatataccca tttcaccgtg ttttctgcac ctaaagctgt tttatgaatt tcagcattag
     4741 gaggggtggc tgtaatacca caaagataaa tctcttctat caaaaaagca tcacaagttc
     4801 taaaaatagc accaatattg tgtagacttc gtatattatc caaaataaca acaagtggca
     4861 gtttagtttt cgactgaaat tcttctacac taatgcgatt taactctgtt attttccttt
     4921 tttgcatggt atcatgaaat aatttgcaca aaaatagcgt aaaaataaga ataaacatat
     4981 ctttttttta catttgcaca agaaaaaagc aagaaagtaa aacagatgaa tgcttgctta
     5041 tttttttaac tactatattt attagaatag aaacatgaaa cagtcgttga ttataattaa
     5101 agtgggaggt aaaattgtag aggaatcagt tagtttagct tctcttttga aagattttgc
     5161 acatacagaa ggatataaag tcttggtaca tggaggtgga cgctctgcta ctaaactgag
     5221 cgagaaatta ggtatagaaa gcaaaatggt agacggaaga agaattacag acaaacagac
     5281 cttggatgta gtaacaatgg tttatggtgg actggttaat aagaatattg tagcaggact
     5341 acaagcattg ggggtaaatg cgttaggact aacgggggca gatatgaata ttatagaaag
     5401 tgttaaacgc ccaataaaag aagtagacta tggttatgta ggagatgtga agcatgtaaa
     5461 tgctcagata gtagcctcat taatacagca aggtatagta cccatagtag ctcctcttac
     5521 gcatgatgga gctggttcta tgctaaatac aaatgcagat actattgcta gtgagttggc
     5581 aaaatcatta tccacttatt ttgatgtaac gttagtgtat tgctttgaga aaaatggtgt
     5641 tttatccgat gaaatggatg ataatagtgt tattccgcta attactcctt cattatttaa
     5701 caaatatgtg caccaaggaa taattaaggg agggatgata cccaagcttg aaaatgcttt
     5761 tcaagccata caagcgggag tgagtaaagt agttataacc aacgctacag ccataggcaa
     5821 agatgctgga acagtagtac taacagatta gctatatgaa agcaataatt attaatccaa
     5881 ttgaactatt aacatcatta cttgttatac cgtcaattag taaaagtgaa caagaaaaat
     5941 ctgattttct tgttaatttt ttaaaacaac atgattgtga tgttcaacgc cataaaaaca
     6001 atctcttttg ttttgctaaa tcgtatgatg aaaacaaacc tacgctaatg ttaaattcgc
     6061 atatggatac tgtgcgtccg agcgattcgt ggacatttcc tccttatcag ccaacactgc
     6121 ttgacggacg aattattggc ttaggagcta atgatgccca tgcatcttta gtatcattaa
     6181 tcactgtttt tttaatgtta aaagacacag aacaatccta taatttatta ttagccattt
     6241 cggctgaaga ggagatttct ggagtaaatg gtatggaaag cttattaacc gtattaccaa
     6301 aaattgattt tgcaatagta ggagaaccta cccaaatgaa tttggcggta gcagaaaaag
     6361 ggctgatggt gcttgattgt gtcgccaaag gacaaagtgg tcatgctgca cgcgaagaag
     6421 gcgtaaatgc catttataag gcaattcgcg atattgaatg gtttaaaaat taccaattcg
     6481 aaaaagaatc tgatttattg ggagcagtaa aaatgtctgt aacaatgatt caggcgggca
     6541 aacagcataa tgtagtgcct gatgcttgtg cgtttacagt ggatatccgt gtcaacgagt
     6601 gttattccaa tcagcaggta ttcgacattg tgaaacaata tgtagaagca gatgttacac
     6661 cacgatcttt tcgtcttaat tcttctgcta tagatttaaa ccatgtgttt gtaaagagat
     6721 ttctttcgat aaacatttat gcaatttcca agtgtaaaaa ttggtgtagg ggattcagcc
     6781 cgctctcata cggcagatga atatattttt atttctgaaa tagaagaagg aataggttta
     6841 tataaacaat taattgatgg tttaactctc aggtaatagg tgtttatatt ccttgccaaa
     6901 gcgtttaagt tctctgataa gagacgagga gatgtgagca tattttaaat cagaaaataa
     6961 aaatacggtt tcgatttcaa ataactcttt attaatatca gccatatttt tttcgtactc
     7021 ataatcgctt attcctctaa ttcctctaac aatacaagat gctttttctg cttttacgat
     7081 atctgcggtt aaattagtat aggtaattac ttttacattc tcatttttaa a
//
LOCUS       DAOSMY010000028         7129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_10545_length_7129_cov_3.828103, whole genome shotgun sequence.
ACCESSION   DAOSMY010000028 DAOSMY010000000
VERSION     DAOSMY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10545_length_7129_cov_3.828103"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLD76_01270"
     CDS             <1..330
                     /locus_tag="PLD76_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU26669.1"
                     /translation="NDKLASIATQHSHDLFTYTSKNENKFVYLSHISTDGSTLDDRLK
                     KANVTANNIRENIGYVQGNERIIVDYWINNPESCKNLMHPTAASIGIGRSGNFWTMLL
                     TSAFITK"
     gene            388..1647
                     /locus_tag="PLD76_01275"
     CDS             388..1647
                     /locus_tag="PLD76_01275"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU26670.1"
                     /translation="MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAMPTLK
                     KILADGGSVIIASHMGRPKKNPDAKYSLQYILKHVEKLLGVPVKFAADCAKASDMAAS
                     LKNGEALLLENLRFYAEEEGKARGLAEDATDEEKAIAKKAIKESQKEFTKTLASYADC
                     YVNDAFGTAHRAHASTALIAKHFDENNKMFGYLMEKEVLAVQKILSDIERPFTAIMGG
                     SKVSSKIEIIENLLGKVDNLILAGGMTYTFSKAMGGNIGISICESDKLDLALELIEKA
                     KKNNVNLVLAVDAKIGDDFSNDCNTQFVSVKEIPDGWEGLDIGPETEKIFAEVIKNSK
                     TILWNGPTGVFEFENFTSGSKAVANAIVEATKKGAFSLVGGGDSVACINKFGLADQVS
                     YVSTGGGALLEAIEGKVLPGIEAIRGF"
     gene            complement(1703..2152)
                     /locus_tag="PLD76_01280"
     CDS             complement(1703..2152)
                     /locus_tag="PLD76_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOU26671.1"
                     /translation="MLEFELPRYTFRVKKQAEKTYILDAYRAKYVRATPEELVRQQMC
                     AYLQNERAYPKTMIFNECVLRINTMNKRCDTVIYSTAHTPIMLIEYKSPDIQISQAVF
                     DQVMLYNRVLQVPYILISNGKQHFCCKLDTDLQQYIFLNEIPVYNNL"
     gene            2189..2962
                     /locus_tag="PLD76_01285"
     CDS             2189..2962
                     /locus_tag="PLD76_01285"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOU26672.1"
                     /translation="MKTKQEIIQNWLPRYTERPLKDFTDLILLTNFNNYVDIFAEWYN
                     VPVLAKNGMMRNASAEGITIINFGMGSPNAALIMDILPAIKPKAVLFLGKCGGLRDKN
                     RLGDYILPSAAIRGEGTSNDYFPPEIPALPAFSLQRAVSSNIRNMEKDYWTGTVYTTN
                     RRVWEHDEAFRAYLMKTRAMGIDMETATLFSAGFYNGIPIGALLLVSDMPLQKDGIKT
                     SDSDKHVTANFVEEHLLIGIKSLSSIKRQSNTIKHLRYE"
     gene            2974..3990
                     /gene="holA"
                     /locus_tag="PLD76_01290"
     CDS             2974..3990
                     /gene="holA"
                     /locus_tag="PLD76_01290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU26673.1"
                     /translation="MAKKTLTYTEIITSLQAKKFAPIYLLMGEEPYYIDLISDYIEKN
                     ILTEDEKMMNQVVRYGKDSDVGSIINEARRFPMMATHQVIIVKEAQMLDKIEQLEMYL
                     QKPLETTILVINHKYKSADKRKKWVTLAEQCGVVFESNKLRDYQMPAMIEQMAREKGM
                     QINEKAKAMLTDFVGTDLAQMAGVIDKLRIILPKDNNQITPEIIELNIGISKDFNAFE
                     LTNALIRRDVLTANRIVHYFSQNEKEHPIQATLAVLFNFFSNLMCFHFLPNQQDDYVA
                     KELKIHSFFVKDYKYAAKAYNKTQVFKIISLLREYDAKSKGIKNSSASGGELLKELVY
                     KIMH"
     gene            complement(3987..4664)
                     /locus_tag="PLD76_01295"
     CDS             complement(3987..4664)
                     /locus_tag="PLD76_01295"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU26674.1"
                     /translation="MTFLPYNQDVDATVKEIMIKIRVAMNGVTSELMSDRGVKYKTNF
                     GVAFPDLQKIAQQYSPNELVAKHLWHKSVRETKILAILLYPAQALTLEKSITWISDCD
                     TVELIEYLCAHLVVFTPFVPTLITQLARISEEKQIIAAYTLAAHYCKRNKPELTEEFS
                     PLLSFSSIHITAKLAQSMLYFLLYAYQKNEFKENIINEMQNLKKNQSENHVAMWLYTE
                     FEALSQD"
     gene            4795..4870
                     /locus_tag="PLD76_01300"
     tRNA            4795..4870
                     /locus_tag="PLD76_01300"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4830..4832,aa:His,seq:gtg)
     gene            5164..6309
                     /locus_tag="PLD76_01305"
     CDS             5164..6309
                     /locus_tag="PLD76_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU26675.1"
                     /translation="MLQRYLDTYWVNLLLLKSVVISVLFISSCSTEEPTPKPSTLCNI
                     SEISFESEQSAMLITVNTKESWSIEHLSNWIDFTATTGRGKTSLLVGATKNDGFGRID
                     SFLIKTAKQTHKIYIEQKGAAIVHLDILSHTLVFVKVEGGTFKIGNYQNPHEHDVSLS
                     EYYIGQTEVTNAFWEAIVGSLPYSNENKNYPYTMDDDNPDFPVTAVSWNDIHTTFLPL
                     LHTKTSHTFRLPTEAEWEFAALGGNKSKGYTFSGSNILDVVGWDASNANDTKQAVAQL
                     EPNEISLYDMSGNAAEWCSDWYSDWYNTTEDTTNPTGPVSGTYKVIRGGSIASETGIL
                     GFHPCHAKQRSYAHPSGFQGAWGDTGHPDEPVIYKCNEIGFRLVLVR"
     gene            6346..6990
                     /locus_tag="PLD76_01310"
     CDS             6346..6990
                     /locus_tag="PLD76_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26676.1"
                     /translation="MFQIKLHTLFCIILMVSMSSPFVSIAQTVIKMELPKQTEKAFAV
                     KTLFNEQLPTGDLVVLGALGYEIEGGATPYELAWYENNQLIAKGNIVAFKPQESAQYS
                     LVVKDKNNCSKTLSINVDALKRAINDTQKPSNFVSLSPTLVKNHIMLRCHSEPIASVH
                     LKFIDINRNIQFISEQGRSIAQELIFLIEAKIEKHKKEEMADDIFGKEDEEEGE"
BASE COUNT         2427 a         1225 c         1308 g         2169 t
ORIGIN      
        1 aacgataaat tagcaagcat agccacccaa catagccacg atttatttac atatacaagc
       61 aaaaatgaga ataaatttgt ttatctcagt catataagta cggatggctc tactcttgac
      121 gaccgtctta aaaaagcgaa cgttaccgcg aataacatac gcgaaaacat tggatatgtt
      181 caaggaaacg aacgtatcat tgttgattat tggataaaca acccagaatc atgcaaaaat
      241 ttaatgcatc caacagctgc cagcataggc attggacgca gtggaaattt ttggacaatg
      301 ttattaacaa gtgcttttat tacaaaataa tttttttacc tttgtgcgtt ttacaaaaga
      361 aataacttaa cctaataata aactgttatg caaacaattg acaattttaa ttttgcagga
      421 aagaaggcgt ttgtacgcgt tgacttcaat gttccgttag acgaaaactt taacattacc
      481 gacgataccc gtattcgtgc ggctatgcct acgttgaaaa aaatccttgc cgatggtggt
      541 agcgttatta ttgcatcgca catgggcaga ccaaagaaaa atccagatgc aaagtattct
      601 ctacaataca ttcttaaaca cgtagaaaaa ttactaggtg tacctgtaaa atttgctgcc
      661 gattgtgcaa aagctagcga tatggctgct tcgctaaaaa acggagaagc attattactc
      721 gaaaatcttc gtttttatgc cgaagaagaa ggcaaagctc gtggattagc cgaagatgct
      781 actgacgaag aaaaagctat agctaaaaaa gcaattaaag aaagtcaaaa agaatttacc
      841 aaaacattgg catcttatgc tgattgttat gtaaacgatg cttttggtac tgctcaccgt
      901 gctcacgctt caactgcgtt gattgcaaaa cactttgacg aaaacaacaa aatgtttggt
      961 tatttgatgg aaaaagaagt attggctgtt caaaaaatac tatcagatat cgaacgtcct
     1021 ttcaccgcta ttatgggagg ttctaaagta tcttctaaaa ttgaaattat cgaaaacctg
     1081 ttgggtaaag tagataattt aattcttgct ggtggaatga cttatacctt tagcaaagca
     1141 atgggtggta atatcggtat ttctatctgc gaatcagaca aattagactt agctcttgag
     1201 ttaatagaaa aagcaaagaa aaataatgta aacttggtat tggctgtaga tgctaaaatt
     1261 ggtgacgatt tttctaacga ctgtaacaca caatttgtgt ctgttaaaga aattcctgat
     1321 ggatgggaag gtcttgacat tggtccagaa actgaaaaaa tatttgcaga agtgattaaa
     1381 aactctaaaa caatcttatg gaatggtcca acaggtgtat ttgagttcga aaactttaca
     1441 agtggctcta aagctgtagc gaacgctatt gttgaagcta ctaagaaagg tgccttctca
     1501 cttgttggtg gtggcgactc tgttgcttgt atcaataagt ttggattggc tgaccaagta
     1561 agctatgtat ctacaggtgg tggtgcgtta cttgaagcta tcgaaggaaa agttcttcct
     1621 ggtattgaag ctattcgtgg tttctaactt aagattccct acttttatca taaaaatgct
     1681 atccacatgg gtagcatttt ttttacaaat tattgtagac aggtatctcg ttcaaaaaaa
     1741 tgtattgctg caaatcagta tctagtttac aacaaaaatg ttgtttccca ttgcttataa
     1801 gaatgtatgg aacttgcaac acacgattgt atagcatcac ttgatcaaat accgcttggc
     1861 ttatctgtat gtcaggtgat ttgtattcta ttagcataat tggtgtgtgt gctgtggaat
     1921 aaattactgt atcgcaccgc ttattcattg tgtttatacg taatacacat tcgttaaaaa
     1981 tcattgtttt aggataagct cgttcatttt gtaaatacgc acacatctgt tgacgcacaa
     2041 gctcttcggg cgtggcacgc acatacttag cacgataagc atccaaaata tatgtttttt
     2101 ccgcctgctt ttttacacga aaagtgtaac gaggtaattc aaattccaac atttttagta
     2161 cttttgctgt aattaacacc gcaaaggtat gaaaacaaaa caagaaatca tacaaaattg
     2221 gctaccacga tatactgaac gtccattaaa agactttaca gatcttattt tgttgacaaa
     2281 tttcaataat tatgtagata tttttgctga atggtacaac gttcctgtgt tagcaaaaaa
     2341 tgggatgatg cgcaatgctt cggctgaagg cattaccatt atcaattttg gtatgggaag
     2401 cccaaatgca gctctgataa tggatattct acctgcaata aaaccaaaag ctgttctatt
     2461 tcttggaaaa tgcggtggat tgcgtgacaa gaatcggctt ggcgactata ttttacctag
     2521 tgcagctata cgaggcgaag gaacgtctaa tgactatttt cctcccgaaa tacctgcatt
     2581 acctgcattt agtttacaac gggctgtttc gtcaaatatt agaaacatgg aaaaagacta
     2641 ttggacagga actgtgtata cgactaatag acgcgtttgg gaacacgacg aagcatttag
     2701 agcttactta atgaaaacaa gagccatggg tattgatatg gaaacggcta ccctattttc
     2761 tgcgggtttt tacaatggaa ttcctattgg agccttattg ttggtgtctg atatgccttt
     2821 acaaaaagat ggtataaaaa cgagcgatag cgacaaacat gtaacagcca attttgtaga
     2881 agaacacctg cttattggaa ttaaatctct ctcttctatc aaaaggcaaa gtaatactat
     2941 caaacattta cgttacgaat aagatacaga actgtggcaa aaaagactct tacatatacg
     3001 gaaattatta cttctttgca ggcaaaaaaa tttgccccta tttacctgtt aatgggtgaa
     3061 gagccttatt acattgacct catttccgat tacatcgaaa aaaatattct taccgaagat
     3121 gaaaaaatga tgaaccaagt cgttcgttac ggaaaagatt ctgatgtggg aagtattatc
     3181 aatgaagcta gacgttttcc catgatggct actcatcaag tcatcatagt aaaagaagct
     3241 caaatgctag ataaaattga gcaactagaa atgtatcttc aaaaacctct ggagactact
     3301 attttagtta ttaatcacaa atataaatcg gctgataaac gcaaaaaatg ggtaacatta
     3361 gctgaacaat gcggtgttgt atttgaatca aataagctac gagattacca aatgcctgct
     3421 atgatagaac aaatggcacg agaaaaaggc atgcaaataa acgaaaaagc caaggctatg
     3481 cttaccgatt ttgtaggaac agacttagct caaatggctg gagtaataga taaattacgc
     3541 atcatcttgc ccaaagacaa caaccaaatt actcccgaaa taattgaact gaatattgga
     3601 attagcaaag attttaatgc ttttgaattg accaatgcac ttatacgtcg cgatgtactt
     3661 acagccaacc gcatcgttca ttatttttcc caaaatgaaa aagagcaccc catacaagcg
     3721 acccttgctg tgttatttaa ttttttctct aatttaatgt gttttcattt tttgccaaac
     3781 caacaagatg attatgtggc aaaagaattg aaaatacact ccttttttgt aaaagactac
     3841 aaatatgccg caaaagcata caacaaaaca caggtgttta agattattag tctactacgc
     3901 gaatacgatg ccaaatcaaa aggaataaaa aattcttccg cttctggagg cgaattacta
     3961 aaagaattgg tttacaaaat tatgcattag tcttggctaa gggcttcgaa ttcggtatac
     4021 agccacattg ccacatggtt ttctgattga ttctttttca gattctgcat ttcattgata
     4081 atgttctcct taaactcatt tttctggtaa gcatacaata aaaaatacag catcgattga
     4141 gccaatttgg ctgttatatg gatggaagaa aatgataaaa gtggagaaaa ttcttctgtc
     4201 agttctggtt tattacgttt acaatagtga gcagccaagg tatacgcagc aattatttgt
     4261 ttttcttctg aaatacgggc aagttgtgtt atcaaagtgg gaacaaaagg agtaaatacc
     4321 actaaatgag cacataagta ctctattaat tccactgtat cgcaatcaga tatccacgtt
     4381 atagattttt cgagagtgag tgcttgtgca ggatacaaaa gaatggctaa aatttttgtc
     4441 tcccttaccg acttatgcca tagatgctta gctactaact cattaggaga atattgttgg
     4501 gcaatcttct gcaaatcggg aaaagcaacg ccaaaattcg ttttatactt tacgccccta
     4561 tccgacatta attcggaagt aacgccattc attgctactc gaatttttat cattatctct
     4621 tttacagtag catcaacatc ttggttatat ggtaaaaatg tcataattaa gttattttta
     4681 aatgacaaag atagtattta gctcgtagct taaaaaaaat acagctaaaa aaaacgctta
     4741 atactattgc ataaaatcaa acttagtagt atctttgcaa cgtttttaac acacggtgat
     4801 tgtagctcag ttggttagag cgtcggattg tggttccgaa ggtcacgggt tcgagccccg
     4861 cctttcaccc ttaaaaaagc aagtaacaaa taacacacga ttattgttgc ttgctttttt
     4921 gatttatgag ctacttcagg ctatattatt cttacttcta caactatttt catacatact
     4981 tttagaaaat agaatttaca cagagggtat cgaatgtatt ttagttaaca aacattgaag
     5041 attgttaata aatatctact ttttattatt aattattcaa tcaaaatttg tagctttgtg
     5101 atactatagt tgtattggac tattatatag cttgcaccat aacaagtata attgttttta
     5161 gatatgttac aacgatactt agatacatat tgggttaatt tactattgct gaaaagcgta
     5221 gtgataagtg tattattcat atcttcttgt tctacagaag aacccacccc caagccttct
     5281 actttgtgca atatctctga aatttcgttt gaatcagagc aatcagctat gctcattaca
     5341 gtaaatacga aagaaagttg gtcgatagaa catctgagca attggataga ttttactgca
     5401 acaacaggac gtggcaaaac ttctctttta gtaggtgcaa ccaaaaatga tgggtttggt
     5461 cgcatcgact cattcttaat taaaacggca aaacaaacgc acaaaatata tattgaacaa
     5521 aaaggagcag ccattgtgca tcttgatatt ttatcccata cactcgtttt tgtgaaagtt
     5581 gaaggcggaa catttaagat agggaattat caaaacccac acgaacatga tgtaagctta
     5641 agcgagtatt atattggtca gacagaggta acaaacgctt tttgggaggc tattgtaggc
     5701 tcactaccat atagcaacga aaataaaaat tacccctata caatggatga tgataatcca
     5761 gattttcctg ttacagcagt ttcatggaac gatattcaca ctacatttct acccctatta
     5821 catactaaaa cttcgcatac atttaggcta cctacagaag ctgaatggga atttgcagca
     5881 ttgggaggaa ataaaagcaa aggatataca ttttcgggta gtaatatact tgatgtagta
     5941 ggttgggatg catcaaatgc taacgataca aaacaagcag tagcacaatt agaacccaat
     6001 gaaataagcc tatatgatat gagtggcaat gccgctgagt ggtgttcgga ttggtatagt
     6061 gattggtaca atacaacaga agatacaaca aacccaactg gacctgtttc tggcacatat
     6121 aaagtaattc gtggtggaag tattgcatct gaaacaggaa ttcttggttt tcatccttgt
     6181 catgcaaaac aacggtctta cgcccatcca tctggttttc aaggagcttg gggagataca
     6241 ggacatcctg atgaaccagt tatttataag tgcaacgaga ttggttttcg cttggtattg
     6301 gtacgataat attcaacaat agtgatcatg tttataagta agcatatgtt tcaaatcaaa
     6361 ttacatactc tattttgtat catacttatg gtaagcatga gtagcccttt tgtatccatt
     6421 gcccaaactg taatcaaaat ggaacttcca aaacaaacag aaaaagcgtt tgcggtaaaa
     6481 acactcttta acgaacaatt acccactggc gatttagttg ttttaggtgc cttaggttat
     6541 gaaatagagg gaggtgcaac accctatgaa ttagcttggt acgagaacaa tcaacttata
     6601 gcaaaaggca atatagtagc atttaagccg caagaaagtg cccaatattc tcttgttgtg
     6661 aaagataaaa ataattgttc taaaacctta tccatcaatg tagatgcatt aaaaagagct
     6721 attaatgata cgcaaaagcc aagcaatttt gtatctcttt caccaacttt ggtaaaaaac
     6781 catattatgc taaggtgtca ttctgaacct attgcttcag tacacctcaa atttattgat
     6841 ataaacagaa atattcaatt tatatcagag cagggacgca gcattgctca agagctaatc
     6901 tttttaattg aagcaaaaat cgaaaagcat aagaaagaag aaatggcaga cgatattttt
     6961 ggaaaagaag acgaagaaga aggggaatag agttcaaaat tgataatctg aatcctaaga
     7021 tatagcaagt gcttcattat aatggtaatg acataactca agcgtcattc tggcgtatgc
     7081 cagaatccat cttacttttt agttattcgt cattggttat tagttctta
//
LOCUS       DAOSMY010000029         6999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_10813_length_6999_cov_3.256336, whole genome shotgun sequence.
ACCESSION   DAOSMY010000029 DAOSMY010000000
VERSION     DAOSMY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10813_length_6999_cov_3.256336"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLD76_01315"
     CDS             <1..673
                     /locus_tag="PLD76_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOU26677.1"
                     /translation="GMGEQATQYKYDFYRTGNNPSYAVANNTQNRYIESITTTNGNIL
                     YNIGVQNGACQYTQSVNITTIPSPVIQVEESSKGVKTIVENETHPPYLYRYDNGNWQT
                     SPWFHLHEKGTYTVTVANARGCERTVPFIVNRALDIIPALFFSPNNDGVNDTWEIENI
                     GHYPHAIIEIYDRFSKLLITYKGNETGWDGMYLGYPMPMTDYWYIITIPELGKPRTGH
                     FTLKR"
     gene            complement(735..3014)
                     /locus_tag="PLD76_01320"
     CDS             complement(735..3014)
                     /locus_tag="PLD76_01320"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOU26678.1"
                     /translation="MAKVNKEDALRYHAEGKPGKIEVVPTKPYSSQRDLSLAYSPGVS
                     EPCLEIEKDASKAYDYTAKGNLVAVISNGTAVLGLGDIGAIAGKPVMEGKGLLFKIFA
                     GIDVFDIEVNEKDPKKFIEVVKAIAPTFGGINLEDIKAPECFEIEDALKRDLDIPIMH
                     DDQHGTAIISAAGLINALELTNKNIEDVKIVVNGAGAAANSCASLYIQLGAKLENIVM
                     LDSKGVINKQRTDLNDRKKPFATDRTIKTLTEALAGADVFLGLSVEGVLSADMIRTMN
                     QNPIVFALANPNPEISYEEAMSSREDIIFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     TKINDEMKVAAVKAIAELTKRSVPDVVSAAYNLKKISFGREYIIPKPLDPRLLTIVAP
                     AVAKAAIESGVARKTIDNWEAYDAKLKELMGRDNKILRYIYETARQQPKRVIFSEANH
                     INMLKAAEASLKEGICFPVLLGNEEKIARIAAENHINLEGIEIINLRHDREENRRIEF
                     AQQLTAKRYRQGMTFEDAYEKMYERNYFGMMMVEMGQADALITGVYSKYADTIQVAKD
                     VIGFREGLNHIGAMHILNTKKGIYFFADTLINRNPTHETLKDIVKLTHSSVQLFGYDP
                     VMAMVSYTNFGAEPTGSPAAINKVVQEIHNEHPEIVIDGEMQVNFALNTELRDKKYPF
                     SKLTGKKVNTLIFPSLSSSNSAYKLLLEMGMADSIGPIQMGLKKPIHILDIESSVRDI
                     VNVTAVAVIDALMEEKKKQ"
     gene            complement(3176..4156)
                     /locus_tag="PLD76_01325"
     CDS             complement(3176..4156)
                     /locus_tag="PLD76_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOU26679.1"
                     /translation="MKAITQNSIGNAETMYIGEVDIPVLRENEVMVQVKAFGVNRADI
                     LQRKGKYPSPAGASPLMGLEVAGIVAEAPVSSPWKIGDKVFGLIPGGGYASYVAIDAE
                     MLWRMPDTMSFEEAAAIPEAFLTAFLALQWQGKLQRGQKVLLHAASGGVGTAAIQLAN
                     AMQAEIVVTASASKHAICAKLGATVCIDYKTESWVDVVQEVMGKNSIDVVVDFLGASY
                     FNDNISVLAMDGRMVMLALMGGADVSGVDLRKIIGKRITVIGSTLRNRPLSFQRKLAR
                     EFAEQFLPSFSDGRLQPIIDTVMDWTLIKEAHERMESNQHVGKIVMKVSE"
     gene            complement(4282..5490)
                     /locus_tag="PLD76_01330"
     CDS             complement(4282..5490)
                     /locus_tag="PLD76_01330"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOU26680.1"
                     /translation="MSSNQKATAWVVTSSAMGINLILGLLYSWSVIKAALVAEGWTNT
                     EASLPYTVCVALLAFMTVFGGKLQDKFGPRVVALGGGILFGVGIFLSAFVHTPWMMVL
                     TFGVVAGLGMGFAYSAATPAAIKWFEPRKKGLIAGIVVAGIGISSIYMAPLTNYLLQG
                     NTVQGTFMILGIIAIVFLSIFGAILHNPPANYIPKAKEGAKVVAHSADFTWQAMMKRK
                     PFYLLWATYFLSATAGLMLIGHIVSIARVQTGNPELNAFWLVMLFAAFNTSGRVLGGF
                     LSDKMGRTTGLLIVFLLQAINMFVFSFYTSFPLLVVGFAVAGIAYGALFALFPAVTAD
                     FFGVKNLGVNYGIIFTSWGIAGIVGPILGGVVADITGTYAGSYMVAGIMLLLGALLVK
                     LIKTPQQEIK"
     gene            5766..6575
                     /gene="zupT"
                     /locus_tag="PLD76_01335"
     CDS             5766..6575
                     /gene="zupT"
                     /locus_tag="PLD76_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOU26681.1"
                     /translation="MIDEKYLFAFALTLFAGLATGIGSLMAFFSKKFNPNFLSAALGF
                     SAGVMIYVSFVEIFFKAKESLTASLGDVTGYWMTVVSFFAGIALIALIDKLVPSGENP
                     HEISNIEEANYKNSTSNDKLLRMGLFSALAIAIHNFPEGLATFTAALTDPTLGISIAV
                     AIAIHNIPEGVAVSVPIYYATKNKKKAFWMSLLSGLAEPVGAIIGFIILRPFLDGATL
                     GIIFGAVAGIMVYISLDELLPTAREYGKHHIAIGGLIAGMAVMSVSLLLFA"
     gene            6662..>6999
                     /locus_tag="PLD76_01340"
     CDS             6662..>6999
                     /locus_tag="PLD76_01340"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOU26682.1"
                     /translation="MKKQEKISLYTHTTLIDGQNRFYKVYVPSHIKNNQTIPLVIALH
                     GNGGNAEALIRETKWHTKASSEGFLVVAPEASRPNPNKPRNQRDNRQTWNDGSGRFHS
                     GERSIDDVKF"
BASE COUNT         2200 a         1436 c         1125 g         2238 t
ORIGIN      
        1 aggtatgggc gaacaagcaa ctcaatacaa atatgatttt tatcgtacag gcaataatcc
       61 tagctatgct gtagccaaca acacccaaaa caggtatatt gaaagtatta caactacaaa
      121 tgggaatatt ctttataata taggcgtaca aaatggggct tgtcaataca ctcaatcggt
      181 taatattacg accataccct ctcctgtcat acaagtagaa gaatcttcaa agggagtgaa
      241 aacaatagta gaaaatgaaa ctcatcctcc gtatctatat cgttatgata atggaaattg
      301 gcaaacatca ccttggtttc atttacatga aaaagggaca tacacggtta ctgtagccaa
      361 tgctcgtggc tgcgaaagaa cggttccgtt tatcgtaaat agagctttag acatcattcc
      421 tgctttattc ttttctccaa ataatgatgg ggtaaacgat acatgggaaa tagaaaatat
      481 tggtcattac ccacacgcca ttattgaaat ttacgaccga tttagtaaat tgctaataac
      541 gtataaagga aacgaaactg gttgggatgg aatgtattta ggttatccaa tgccaatgac
      601 agattattgg tatattatta ccatacccga gttgggcaaa ccccgcactg gtcactttac
      661 gttaaaaaga taaataaata aacaaagaaa gcaggtaaaa taacctgctt tctttgtata
      721 aaaccatatt tgtattattg tttctttttc tcttccatca aggcatcaat cactgctact
      781 gctgttacat taacgatatc tcttaccgaa ctttcaatat ccaaaatatg aataggtttt
      841 ttaagcccca tttgaatagg accaatactg tcagccatgc ccatttcgag caataacttg
      901 tatgcactgt ttgacgaact taaacttgga aaaatcaagg tatttacttt tttcccagtc
      961 agttttgaga aaggatattt tttatcgcgt aattctgtat ttaaggcaaa attcacttgc
     1021 atttcgccat caataactat ttcaggatgt tcattgtgaa tttcctgtac tactttattg
     1081 attgcagctg gacttcctgt tggttctgca ccaaagttag tatacgaaac cattgccatg
     1141 acaggatcgt atccaaatag ttgaactgaa ctgtgtgtta gttttacaat atcttttaat
     1201 gtttcatgcg ttggattgcg attaatgagt gtatctgcaa aaaaataaat cccctttttt
     1261 gtgttcaaaa tatgcatagc accaatatga tttaatcctt cgcgaaaacc gatcacatct
     1321 ttagctactt gaatagtatc cgcatacttt gaatatacgc ctgtaatcaa agcatcagcc
     1381 tgacccatct cgaccatcat cataccaaaa tagtttcttt catacatttt ttcgtatgcg
     1441 tcttcaaacg tcatcccttg acgataacgt ttggctgtta actgttgagc aaactcaatg
     1501 cgtcgatttt cctccctatc gtgacgtaaa ttaataatct caataccctc taaattaata
     1561 tgattctcag cagcaatgcg tgcaattttt tcttcatttc ccaataatac aggaaaacag
     1621 ataccttctt tgagtgatgc ttctgcagct ttgagcatat taatgtggtt ggcttccgag
     1681 aaaataactc gcttaggctg ctgacgagcc gtttcgtata tatagcgtaa tatcttatta
     1741 tcacgcccca ttagttcttt tagttttgca tcatacgctt cccagttgtc aatggttttg
     1801 cgagctaccc ccgattcaat agcggctttt gctacagcag gagctacaat ggttaacaaa
     1861 cgaggatcta agggtttggg tataatatat tctctaccaa aactaatttt tttaagatta
     1921 taagccgcac ttaccacatc aggcactgac cgttttgtta attcggctat cgctttcacg
     1981 gcagcaactt tcatttcgtc atttatcttg gttgcacgta catcgagtgc tcccctaaaa
     2041 atataaggga aacccaacac attattaatt tgattaggat gatccgatcg acccgtagca
     2101 aaaatgatat cttcacgcga agacatagct tcttcgtagg atatttcagg atttggattg
     2161 gctaatgcaa acacaatagg attttggttc atcgtacgaa tcatatcagc cgaaagcacg
     2221 ccttctacgg ataaacctaa aaagacatca gctcctgcta aagcttctgt taatgtttta
     2281 atcgttctat ccgttgcaaa tggctttttt ctatcattta aatccgtacg ttgtttattg
     2341 ataactcctt ttgaatcaag catcacaata ttttcgagtt ttgctcctaa ctgaatatac
     2401 aatgatgcac acgagtttgc tgctgctcct gcaccattaa ccacaatttt tacgtcctca
     2461 atatttttgt ttgttaattc caatgcattg attaacccag cagcagaaat gatggctgta
     2521 ccatgttgat catcgtgcat aataggaata tccaaatcac gctttaatgc atcttcaatc
     2581 tcaaaacatt cgggagcttt aatatcttct aagttgatac ctccgaaggt aggtgcaatc
     2641 gcttttacta cttctataaa ttttttaggg tctttttcgt ttacctcaat atcaaacaca
     2701 tcaatacccg caaaaatctt aaacaacaag cctttgcctt ccataacagg ctttccagct
     2761 atggcaccaa tatcacccaa tccgagcaca gctgtaccgt ttgaaattac agctactaag
     2821 ttgcctttag cggtataatc atacgcttta ctggcatctt tttctatttc taaacagggt
     2881 tcagaaacac ctggggaata agctagtgat aaatctcgtt gcgaactata gggtttagtt
     2941 ggtacaactt ctattttgcc tggtttaccc tctgcgtgat aacgtaaagc atcttcttta
     3001 ttaacttttg ccataaatat tatttttgct acacagtagc gattaactta taaattgtaa
     3061 gaaaaaatta tacaaatgta cgtaaatatt tattgataga gcattctact tactacaatc
     3121 catctaagaa gttactattt ttaactcttt ttacacatca tactaagggc gttatttact
     3181 ctgatacttt catcacaatt tttcctacat gttgattcga ctccattcgt tcatgtgcct
     3241 ctttaatgag cgtccaatcc atcactgtat caattatggg ttgcaaacgt ccatctgaaa
     3301 aagatggtaa aaattgttca gcaaactcac gtgctaattt tctctgaaat gataagggtc
     3361 gattacgtaa tgtagagcca atgacagtaa ttcgtttacc aataatcttg cgtaaatcaa
     3421 ctcccgaaac gtctgctcca cccatcaatg ctagcattac cattctgccg tccatagcta
     3481 ataccgaaat attgtcgtta aaatatgaag cccctaaaaa atcgactacc acgtcaatgc
     3541 tatttttacc cattacttct tgtactacat caacccacga ctctgtttta taatcaatgc
     3601 ataccgttgc tcctaactta gcacaaatag catgcttact ggctgatgca gttacaacta
     3661 tttcggcttg catggcatta gccaattgaa ttgctgcggt acctacacct cctgatgcgg
     3721 cgtgtagcaa aactttctgt cctcgttgca actttccctg ccactgtaac gctaaaaatg
     3781 cagtaagaaa agcttcgggt atagctgccg cttcttcaaa actcattgta tctggcattc
     3841 tccacagcat ttcagcatca atagccacat aactagcata cccacctcct ggaatcaacc
     3901 caaacacctt atctccaatt ttccaggggc tcgaaacagg tgcttctgca actattcctg
     3961 ctacttctaa ccccatcaaa gggctggctc ctgcaggaga aggatatttc ccttttcgtt
     4021 gcaaaatatc cgccctattt actccaaatg cttttacctg caccattact tcattttctc
     4081 ttaacacagg tatatctacc tctcctatgt acattgtttc agcatttcct atgctatttt
     4141 gtgttattgc tttcattatt ttacttattt gtattcgttt atgcaaagat acatatctaa
     4201 gctcagaata gtaccttgta gatgcaaaaa aaagacctgc aaactacatg caggtcttcc
     4261 ttaaactatc agtatgtaat attatttaat ttcctgctgt ggcgttttta tcagttttac
     4321 caataaagca cctagcaaga gcataatacc tgctaccata tacgaacctg cataagtacc
     4381 tgttatatca gcaaccacac ctcccaatat cggacccact atacctgcaa taccccagct
     4441 agtgaagata ataccataat taacccctaa attttttacg ccaaagaaat cggctgttac
     4501 tgcggggaac aatgcaaata aagccccgta agctatacct gccacggcaa aaccaacaac
     4561 caataatggg aaagaagtat aaaatgagaa aacgaacata ttaattgctt gtaataagaa
     4621 tacaattaac aaacctgttg tacgacccat tttatcagat aaaaatccac ccaacacacg
     4681 accagaagta ttaaatgcag caaaaagcat tactaaccaa aaagcattta actctggatt
     4741 tcctgtttgc actctggcta tacttacaat atgtccaata agcatcaaac ctgctgtggc
     4801 actcaaaaaa taagtcgccc acaacaaata aaatggcttt cttttcatca tggcttgcca
     4861 ggtaaaatca gccgaatgag ctactacctt cgccccttct tttgccttag gtatataatt
     4921 agcaggaggg ttgtgtaaaa tagctccaaa aatgcttaaa aaaacgatgg caataatacc
     4981 caaaatcata aatgtacctt gtacggtatt tccttgtaac aaataattag ttaacggagc
     5041 catgtagata gaagaaattc caattcctgc aacaacaatt cctgcaatta aacctttctt
     5101 gcgaggttca aaccatttga tagcagcagg tgttgctgcc gaataagcaa aacccattcc
     5161 caaaccagct accacaccaa atgtaagtac catcatccaa ggtgtatgta caaatgcaga
     5221 caagaaaata cccacaccaa ataatattcc accacccaaa gctacaacac gtggaccaaa
     5281 cttatcttgt aatttacctc caaaaacagt cataaaagct aataatgcaa cacatactgt
     5341 ataaggcaaa gaggcttctg tatttgtcca accttctgct actaatgctg ctttaattac
     5401 actccatgaa tacaataatc ctaaaattaa attaatcccc atagcagagg atgttaccac
     5461 ccaagctgtc gctttttgat ttgaactcat tgattttttg attttaatgt tttagttact
     5521 agtaattata taaatagttc ttcgaacaca gaataaggca ttttacaaaa tcgatgcaaa
     5581 gaaaactatt ttattttaat tagttagtat tttaccgaaa aatacattaa taaaaacaac
     5641 gcaaacgttt gttttcttac aatcaaacaa aataaaaatg aattttagct taacaagata
     5701 aaaaggtttt tttattgcaa tatttttcat acttttgatg taaaattata gactattgac
     5761 gtatgatgat tgacgaaaaa tatttatttg cttttgcatt aactttattt gcagggttag
     5821 caactggcat tgggagttta atggcttttt tttctaaaaa gtttaatcca aattttttat
     5881 cagctgcctt aggtttttca gcaggagtaa tgatttatgt atcttttgta gagatttttt
     5941 tcaaagcaaa agaatcgctt acggcttcat taggagatgt tactggctat tggatgacgg
     6001 ttgtttcatt ttttgcagga attgccttaa ttgcactcat tgataaactt gttccttctg
     6061 gcgaaaaccc tcatgaaatt agcaatattg aagaagctaa ttacaaaaat agtacgagta
     6121 atgacaaact ccttcgtatg ggtttatttt cagctttagc tattgccata cataattttc
     6181 cggaaggatt agcaacattt acggctgctt taactgatcc cacattgggt ataagtattg
     6241 cagttgctat tgctatacac aatataccgg aaggagtagc agtttcagtt ccaatttatt
     6301 atgcaacaaa aaacaagaaa aaagcttttt ggatgtcatt gttatctgga ttagccgaac
     6361 cagtaggtgc aataatcgga tttattattc tacgtccatt tttagatggt gctacattgg
     6421 gaattatctt tggtgcagtg gctggaatta tggtttatat ttcgcttgat gaactacttc
     6481 ctaccgcacg tgagtatgga aaacaccata tcgctattgg tggtcttatt gcagggatgg
     6541 ctgttatgtc ggttagttta ctattatttg cataaaatgt tcagaaatga aattgcgctg
     6601 tgttaggtat actttatttt tatttttttt cttggttttt acagcttgtg gctctttggt
     6661 agtgaagaaa caagaaaaaa tctctttgta cacacataca acactaattg atggtcaaaa
     6721 tcgattttac aaagtatatg ttcctagcca tataaagaac aatcagacta taccactagt
     6781 tattgctcta cacggcaatg gaggtaatgc cgaggcactt attagagaaa ccaaatggca
     6841 cacaaaggct tcaagcgaag ggtttttagt agttgctccc gaagcttcac gacccaaccc
     6901 aaataaacct cgcaatcaga gagataatcg tcaaacatgg aatgatggtt ctggtcgatt
     6961 tcattctgga gaacgctcta ttgatgatgt aaagtttat
//
LOCUS       DAOSMY010000030         6912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11016_length_6912_cov_3.441884, whole genome shotgun sequence.
ACCESSION   DAOSMY010000030 DAOSMY010000000
VERSION     DAOSMY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11016_length_6912_cov_3.441884"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLD76_01345"
     CDS             complement(<1..217)
                     /locus_tag="PLD76_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26683.1"
                     /translation="MAKRTLDSIHVLFPQEVEERKKALLKKYEIEELELERNFIFYDS
                     IIRVKQTVIDSIEHFFVVKADSFYRGER"
     gene            433..1848
                     /locus_tag="PLD76_01350"
     CDS             433..1848
                     /locus_tag="PLD76_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU26684.1"
                     /translation="MKEYIQNIVFSTIGKCADEMHQECYVIGGYVRDIFLQRPSKDID
                     VVVVGNGIELAERVCKIWGKRARLHIFKNFGTAQIKFQEMEIEFVGARKESYRLDSRK
                     PIVEDGTLVDDQNRRDFTINALAICLNQDRFGELVDPFNGVRDIENKILRTPLNPDIT
                     FSDDPLRMIRCIRFATQLKFHIEENTILALSRNKDRLTIVSKERIADEIQKIMQADKP
                     SIGWLLLDKCGLLPYILPELTAMKGVDTKSGLSHKDNFLHTLEVVDNIAKQTNNVWLR
                     WGALLHDIAKPITKKYVQGTGWTFYNHNYIGEKMVARIFNQMKLPMNEKMKYVQKLVL
                     LHMRPIALVEEGITDSAIRRLLFDAGDDIDDLMTLCEADITSKNEIKVQKFLQNLQLV
                     RKKLKDIEEKDRIRNFQPPISGQEIMEIFQLPACATVGIIKTRIKDAILDGEIPNEYE
                     EAKALTLRYGKELGLTATTQS"
     gene            complement(1835..2527)
                     /locus_tag="PLD76_01355"
     CDS             complement(1835..2527)
                     /locus_tag="PLD76_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU26685.1"
                     /translation="MIVLHTTAQSDRGVVRNLQNVDLEPFHFGFILGLNYSDYTATPS
                     NIVDTNQQIWYPASKGLSPGFTVGMLVDLRLFEYLNLRFTPTLGLGQRDLNFSAFSTT
                     TDERIGNPTIINIKSTTVDLPLLIKYSAERVGNYRPYLLAGAGPTINLVRSKADPILV
                     KPFDVQAYIGFGCDMYLEYFKLCPELKFGFGLLDSLERNHPDMQGTSNQKFIDAISRL
                     SSRMLVLTFNFE"
     gene            complement(2638..3999)
                     /gene="hisS"
                     /locus_tag="PLD76_01360"
     CDS             complement(2638..3999)
                     /gene="hisS"
                     /locus_tag="PLD76_01360"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU26686.1"
                     /translation="MKMKPTIPKGTRDFLPNEMAKRNYIFNTIKEVFRLYGFNQIETP
                     AMENLSTLMGKYGEEGDKLLFKILNSGDYLANVSADELVTKESNKLVNKFSEKGLRYD
                     LTVPFARFVVQYRDKITFPFKRYQIQPVWRADRPQKGRYREFFQCDADIVGSDSLVNE
                     VELIQLIDDVFTRLQLNVLIKLNNRKILSGIAELIGEHDKIVDITVAIDKYEKIGLEN
                     VQKELLEKGLTLHAIETITPIITLSGDNGTKLEMLKKVLATSSVGMEGIQELQTVLNY
                     VAGLNLKTAIEIDLTLARGLNYYTGAILEVKSLDVQIGSITGGGRYDNLTGVFGMQGV
                     SGVGISFGADRIYDVLNQLELYPKEKMHQTNVLVLPFGEKEQVYGLEIVKELRKNGIA
                     TELYPDVVKIKKQLAYADNNGIPYVILIGENEINTQTLTLKNMLTGEQYTGDKDTVFA
                     KIL"
     gene            4201..5022
                     /locus_tag="PLD76_01365"
     CDS             4201..5022
                     /locus_tag="PLD76_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26687.1"
                     /translation="MKLEEIACSRHFLLVSDADYFPINAYACALQFAQGYEKNLSFLV
                     LLTKKQLAHRDVIYETWNRQIETLQKKESIEIKVFFTESYFSELVPFTEQAETPMLFF
                     EISKNTQFNNPLSIFKQLKELRIPFVITKDSQTKPIDLSKIIVPIGFLTEEKEKAPYS
                     CNMGRFLHSEIILYQANDYGSKTTKNVESVLKLYNQFSLKVSIIKAKADSFGVEKEAT
                     MATEKMQAGMVLVSTSRDYGLDDILFGPKELHCLKRATVPIMFINPRPDLYVLCW"
     gene            5135..>6912
                     /locus_tag="PLD76_01370"
     CDS             5135..>6912
                     /locus_tag="PLD76_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOU26688.1"
                     /translation="MSKQSKNQENGVKAPKKPSRFFANLISFFKNEKLHFILGMVLFA
                     FAAFSVLSLLSFLTLGENDYNLLGLSFEELAEQNKQFENFGGVTGAHLANIVINKWFG
                     VSSFLLVFFIISVAYKFLNPTTSYSLFKRFIFSAFFTIWISLAFGFLLPEISQLSFIQ
                     LGGNHGNEMAKILQLSMGYSGVILLLAVSLFMFLIIGFAATLPFFQKIANFNVSSLFR
                     KKKIEETVLENSEEIEAMENSPEENDSETEEFEVFEVEEENEEKEDISFEVIEPTNED
                     AELVEQATNEEEIDSVKLEEVTNENENGVKLVIEEGVKEEIYDKIAELGEYDPTLELP
                     HYKYPTFDLLQKYDSNNKINLEEQSANKNRIIQTLDNYGIKIKEIKATVGATVTLYEI
                     IPEAGVRISKIRNLEDDIALSLSALGIRIIAPIPGKGTIGIEVPNKTSQIVSMHSVIA
                     SKKFQEAKHELPIVFGKTITNEVFTVDLCKMPHILVAGATGQGKSVGLNAIITSLLYK
                     KHPSQLKFVLIDPKKVEFSIYSNIEKYFLAKLPDADEAIITDVTKVVQTLNSLTKEMD
                     SRYDLLKKAHVRTIIEYNNKFVKRTL"
BASE COUNT         2423 a         1123 c         1130 g         2236 t
ORIGIN      
        1 ccctttcacc tctgtaaaaa gaatcagctt ttactacaaa aaaatgctct atactatcta
       61 ttactgtttg ttttacacgt attatactat cataaaagat aaaattacgt tctaattcta
      121 actcttcaat ttcatatttt ttcaatagag ccttttttct ttcctcaact tcttgaggaa
      181 atagtacatg gattgaatcg agtgttcgtt ttgccacctc aatattgttc aataacaata
      241 agctatcaac tctttgcaaa taatgaacac ttgtacttct ttcctgttta ttgcatgata
      301 aaaacaaaat ggacaggaga acacaccacg catacatatt ttttttcata gccttcaaat
      361 caaaggcaaa aataatattt ttttgtcaga ttggtgtaaa gaaagtactt ttgtagggtt
      421 aataatattt aaatgaaaga atatatccaa aatattgttt tttcgactat tggaaaatgt
      481 gctgatgaga tgcatcagga gtgttatgtt attggtggtt atgtcagaga catctttttg
      541 caacgtccat ctaaagatat agatgtagta gtagtgggca atggtattga attagctgaa
      601 agagtatgta aaatatgggg aaaaagagct cgattacata tttttaaaaa ctttggaact
      661 gctcaaatta aatttcaaga aatggaaatt gaatttgtag gagccagaaa agaatcatac
      721 cgattagatt ctcgcaaacc gattgtagaa gatgggacgc tagtagatga tcagaacaga
      781 agagatttta ctattaatgc gttagctata tgtttaaatc aagatcgttt tggtgaatta
      841 gtggatccat ttaatggtgt tcgtgatatt gaaaataaaa ttctacgcac accattaaat
      901 ccagatataa cattcagcga cgacccatta agaatgatta gatgtattcg cttcgcaaca
      961 caactaaagt tccatataga agaaaataca atattggctt tatctcgcaa taaagaccga
     1021 ttaacaattg tgtcgaaaga aagaattgca gacgaaattc aaaaaatcat gcaagcagat
     1081 aaaccttcta ttggttggtt acttcttgat aaatgtggat tattacctta tattttacca
     1141 gaattaactg ccatgaaagg cgttgataca aaatcgggat tatcgcataa agacaacttt
     1201 ttacatacat tagaggtagt agataatatt gctaaacaaa ctaataatgt atggttaaga
     1261 tggggtgctc tgttacacga cattgctaag cctataacaa aaaaatatgt gcaaggtaca
     1321 ggttggactt tttataatca taactatatt ggcgaaaaaa tggtagctcg tatttttaac
     1381 caaatgaagc tacctatgaa cgaaaaaatg aaatacgttc aaaaacttgt attactacat
     1441 atgcgaccca tagcattggt tgaagaagga atcactgact ctgctatcag aagacttctt
     1501 tttgatgctg gagatgatat tgatgattta atgacactat gcgaggctga tattacatca
     1561 aaaaatgaaa taaaagtgca aaagtttctt caaaaccttc aattagtccg caaaaaatta
     1621 aaagacattg aagaaaaaga taggattaga aattttcaac cccccatttc tggtcaggaa
     1681 ataatggaaa tatttcaact ccctgcatgt gctactgttg gtataattaa aacacgcatt
     1741 aaagatgcta ttcttgatgg agaaattcct aatgaatacg aggaagccaa agctttaaca
     1801 ctacgttatg gaaaggaatt agggttaacg gcaactactc aaagttaaag gtaagcacta
     1861 acatacgcga acttaaacga cttatagcat caataaattt ttgatttgag gttccttgca
     1921 tatcaggatg attccgctca agtgaatcca aaagcccaaa accaaattta agttcaggac
     1981 atagcttaaa atattctaaa tacatatcac atccaaaacc gatataagct tgtacatcaa
     2041 aaggtttcac taaaatagga tcggcttttg aacgaactaa attaatagtt ggtccagctc
     2101 cagccagcaa atatggtcgg taattcccta ctctttcggc actatactta ataagtaatg
     2161 gcaaatctac tgttgttgat ttaatattaa tgatggttgg attaccaatt cgttcgtctg
     2221 tagtagtaga aaatgcagaa aaatttagat cacgctgtcc cagtcctaag gttggcgtaa
     2281 aacgcagatt taggtattca aacaatcgta aatcaacgag cattccaact gtaaaccctg
     2341 gagataaccc cttagatgca ggataccata tttgctgatt tgtatcaact atattagatg
     2401 gtgttgcagt ataatctgaa taatttaacc ctaaaataaa tccaaaatga aagggttcta
     2461 aatctacatt ttgtaaattg cgtaccactc ctctatcaga ctgtgctgtt gtatgtaaaa
     2521 caatcaaaga caaaagcagt agtagggata attttttgag catagtttat tttttctatt
     2581 tagtataaca gaaaaatgct actgttattt tattgtgctt gtattaaacg atgatattta
     2641 aagaatttta gcaaaaacgg tatctttatc gcctgtatat tgttcgcctg taagcatatt
     2701 tttaagagtc agagtttgcg tattgatttc attttcgcct atcaaaataa catacggaat
     2761 gccattgtta tcagcataag caagctgttt ttttattttc actacgtcag gatataattc
     2821 agtagctatt ccgttttttc tcaactcttt tacaatttcc aaaccgtata cttgttcttt
     2881 ttcgccaaaa ggtaatacaa gtacatttgt ttggtgcatt ttttctttag gatataactc
     2941 caattgattg agtacatcgt agatgcggtc agctccaaac gaaataccta cgccagacac
     3001 accttgcata ccaaatactc ctgttaaatt gtcgtatcta ccacctcccg ttatactacc
     3061 aatttgtaca tcaagcgatt ttacttcaag aatagctcct gtatagtaat ttaaaccacg
     3121 agccagcgtt aaatctattt caatggcagt cttaagattt agtccagcta cataattgag
     3181 taccgtttgt aactcttgta tcccttccat tcccacagaa gatgttgcta atactttttt
     3241 tagcatttct aattttgtac cattatcacc agataaggta ataataggtg taatggtttc
     3301 tattgcgtgt agcgtaagtc ctttttcaag taattctttt tgaacatttt caagtccaat
     3361 tttttcgtat ttatctattg ctaccgtaat atctacaatt ttgtcgtgct caccaatcaa
     3421 ttcggcaata ccagaaagaa tttttctatt atttagttta attaaaacat tcagttgcaa
     3481 acgtgtaaat acatcatcaa ttaattggat taactctacc tcatttacaa gtgaatcgct
     3541 acctactata tccgcatcgc actgaaaaaa ttcgcgataa cgccctttct gtggtctatc
     3601 agccctccaa acgggttgta tttggtatcg tttgaacgga aacgtaattt tatcgcgata
     3661 ttgcactaca aagcgagcaa agggaacagt taaatcataa cgcaaacctt tctcagagaa
     3721 tttatttact aacttgttac tctctttagt aaccaattca tcagcagaaa cattggctaa
     3781 ataatcgcca gaattcagta ttttaaataa taatttatct ccttcttctc catacttacc
     3841 cattaatgtg gacaaatttt ccatagcagg agtttctatc tgattaaatc cgtacaaacg
     3901 aaatacctct tttatagtat taaaaatata atttcgtttg gccatttcat ttggcaaaaa
     3961 atcgcgtgta cctttgggta tagtaggttt catttttatg tattataatg ttatactctg
     4021 taatactagt cgtatgtata ctgttttctt ggctagatag gtttatacac aaactatcgc
     4081 tacaatattt ttgcgagttg caaaggtaca aaaaaatatg tttttagcat tttatttaga
     4141 caagaaaaag aaaaaacact aactttgtaa accaaaaact aaccatattc gacaaacact
     4201 atgaaacttg aggagattgc ttgttcacga cactttttac tggtatccga tgccgattat
     4261 tttccaatta atgcctacgc atgtgcctta caatttgctc aagggtatga aaaaaatctg
     4321 tcttttttgg tattgttaac aaaaaagcaa cttgcacacc gtgatgttat ttatgaaaca
     4381 tggaataggc aaatagaaac actacaaaaa aaagagtcga ttgaaataaa agtctttttt
     4441 accgaatcct atttttctga attagttcct tttactgaac aggcagaaac tccgatgttg
     4501 tttttcgaga tttcaaaaaa cacgcagttt aacaatccac ttagcatatt taaacaactg
     4561 aaagagctac gtattccatt tgtaattaca aaagatagcc agaccaaacc aattgattta
     4621 tcaaaaatta ttgttccaat aggttttttg actgaagaaa aagaaaaagc cccttattcg
     4681 tgtaatatgg gacgtttttt acattcagaa attattttgt atcaagccaa tgactatggt
     4741 tcaaaaacga ccaaaaatgt agaaagtgta ctaaaattat acaatcaatt ttcattgaaa
     4801 gtcagcatta taaaagctaa agcagatagt tttggtgtag aaaaagaagc cacaatggct
     4861 acagaaaaaa tgcaagcggg tatggtatta gtaagcacat ctcgcgatta tgggttggac
     4921 gatatcctat ttggacccaa agaactgcat tgtttgaaac gtgcaacagt ccccattatg
     4981 tttatcaatc cccgtccaga tttatatgtt ttgtgttggt aagatatgta aaaaataaaa
     5041 aaaaacgttg ttaaaaacta tgtaaaaaat ccttctttgt tcagataaat tttatatacc
     5101 tttgcacttc aaaagaaaaa aaacaccccc ttatatgagt aaacaatcta agaatcaaga
     5161 aaacggagta aaagctccta aaaaaccaag ccgttttttt gcgaatctta tttctttttt
     5221 taaaaatgag aaactacact tcatactggg tatggtattg tttgcttttg cggctttttc
     5281 agtattatcc ttattatcat ttttaactct cggagaaaat gactataatt tactcggatt
     5341 aagttttgaa gaattagccg aacagaataa acagtttgaa aactttggtg gagtaactgg
     5401 ggctcattta gctaatatag taataaacaa gtggttcggg gtttcgtctt ttctattagt
     5461 ctttttcatc atttcagtag cgtataaatt tttgaatcct accacttcat attctttgtt
     5521 caaacgtttt atttttagtg cgttttttac catttggata tcattggctt ttggttttct
     5581 attgccagaa atttctcaat tatcatttat tcaactgggt ggtaatcatg gtaatgaaat
     5641 ggcaaaaatt ttacagctat ctatgggata tagtggtgtg atattgttgc ttgcagtatc
     5701 cttatttatg tttttaatta ttggttttgc tgctacttta ccatttttcc aaaaaattgc
     5761 caactttaac gttagttctc tttttagaaa gaaaaaaata gaggagacgg tactagaaaa
     5821 tagtgaagag attgaagcaa tggaaaattc tccagaagaa aatgattcag aaacagaaga
     5881 atttgaagta tttgaggttg aagaagagaa tgaagaaaaa gaagatataa gctttgaagt
     5941 aatagaaccc actaatgaag atgctgaact tgtagaacaa gccactaatg aagaagaaat
     6001 agactctgta aagctagaag aggtaacaaa cgaaaatgaa aacggtgtaa aactagtaat
     6061 cgaagaaggt gtcaaagaag aaatatacga taaaatagca gaattaggcg aatatgaccc
     6121 taccctggag ttgccacatt ataaatatcc tacgtttgat ttactacaaa aatatgactc
     6181 aaacaacaag atcaatctcg aagaacaatc ggctaataaa aatagaatca ttcaaacgct
     6241 tgataattat ggtataaaaa taaaagaaat aaaagctact gtaggtgcta ccgtaacttt
     6301 atacgaaata ataccagaag caggtgttcg tatttcaaaa attcgcaatt tagaagatga
     6361 tattgcattg agtttgtctg ctttaggtat tcgtattatt gctcctattc caggcaaagg
     6421 tactattggt attgaagtac caaacaaaac atcacaaata gtatcaatgc attctgttat
     6481 tgcttctaaa aagtttcaag aagctaaaca tgaactgcca attgtatttg gaaaaaccat
     6541 tacaaacgag gtatttactg ttgatttgtg caaaatgccc catatattag tggcaggagc
     6601 aacaggacaa ggaaaatcag taggactaaa tgcgattata acatcattac tttacaaaaa
     6661 gcacccttcg caattaaaat ttgttttaat tgaccctaaa aaagtagagt ttagtattta
     6721 ctcaaatatt gaaaagtact ttcttgctaa attgcctgat gcagatgaag ctatcattac
     6781 tgatgtaacg aaggtagtac aaacactgaa ttcgttgacc aaagagatgg atagtcgtta
     6841 tgacttatta aaaaaagcac atgtgcgtac tattattgaa tacaacaata agtttgtaaa
     6901 acgtacactc aa
//
LOCUS       DAOSMY010000031         6873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11105_length_6873_cov_3.452332, whole genome shotgun sequence.
ACCESSION   DAOSMY010000031 DAOSMY010000000
VERSION     DAOSMY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11105_length_6873_cov_3.452332"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2608)
                     /locus_tag="PLD76_01375"
     CDS             complement(<1..2608)
                     /locus_tag="PLD76_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU26689.1"
                     /translation="MKPFLQQVASVFYHTYKEDLTDFCFVFPNRRSGLFFEKYLMETA
                     QHTFFSPTITSISDFCTSHSMLQLEDKMGLLFRLYGIYQSIANKHETFDSFASFGEIL
                     LSDFDDIDKYLVNAEDIFQNVKELQSINYLPDYLTNEQKIIIQRFWNNFNPEPKRQQE
                     FLATWEILFPLYQSFKDDLKKNNLAYEGMLLRELAENGIHASYNKIVFVGFNALSKVE
                     KTLFLQLKKQKKADFYWDYEGEFVQDKTNKAHTYVAENLMLFPSQLKIEKEIIGNKYV
                     EAIAIPSSVGQAKQVHQILQHLGIENISTETAIVLSNENLLLPMLFSLPNHIDKVNIT
                     MGYPLVFTPIKELIDNLLALQKSANTNTEGKTFFYYKQVLSLLSHRYIYNENNTNYQE
                     LIKKIHSNNWIRIPALNLQIDTLLCLLFKPCNTASLCDYLIAIIGHLLAERKSVSINN
                     SDENNQLMEQEFLYQCFIALQRMQDLLNQWNIPLSLDTLSKLIKRLLSTIVISFEGEP
                     LAGLQIMGMLETRCLDFKNLIITSFNEGIFPKNESSPSFIPYNLRKGFDLPTTEHQDA
                     IFAYHFYRLFHRASSVYLLYDTRTEIMNTGEKSRFINQLRYGYGIPIVEKTLTYNTSL
                     AKTNAIRIDKTPAIMDKLHAIVLSPSSINTFITCPLQFYFSYIENIKQTDSVTESIES
                     NIFGSIFHFVMEKLFVHFKDKMISKEIIDTMIRDTSKIELLISEGFSQFYFKDKTPHV
                     LEGNHYLVAQVISKYVRKLLDIESKRTPFFHITHEYKCEENYYISKYKTFAKIKGSID
                     RVQIKDGVIMLIDYKTGSGELKYPSIGELFNGNLKDRRKDILQILLYSWLYQQNKKID
                     SEIECHIIYLRKM"
     gene            2716..4662
                     /locus_tag="PLD76_01380"
     CDS             2716..4662
                     /locus_tag="PLD76_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU26690.1"
                     /translation="MISIEGLTVEFGAKALFDNISYVVNKKDRIALVGKNGAGKSTML
                     KIIAGLQEPTRGQCIIPKDLTIGYLPQHMIHSDDATVYEEAEKAFAHILEIQDQIEKK
                     NILLCERDDYESDSYHSLIEEITHLNEQLAMLGGVNYVADIERTLFGLGFLRTDLARP
                     TKEFSGGWRMRIELAKLLLQRPDVLLLDEPTNHLDIESIQWLENFLASRANAVMLVSH
                     DKAFINAVTTRTIEISLGKIYDYRANYSTYLELRKERREQQLRAYENQQKQIQDTEEF
                     IERFRYKTSKAVQVQSRIKQLDKIDRLEVDEEDTSSLRLKFPPAPRSGSFPVIAEGLA
                     KLYGNYVVFSNLDLTIKRGEKVAFVGKNGEGKSTLVKCIMNEIEYAGKLTLGHNVQIG
                     YFAQNQASLLNESKSVFETIDDVAVGDIRTKIRDILGAFMFGGEEADKKVKVLSGGER
                     SRLAMIRLLLHPVNLLILDEPTNHLDIRSKDVLKQAIKEFDGTVIVVSHDREFLDGLV
                     TKVYEFADKKIIEHIGGVYEFLHKKKLNTVQEIERKNLPNKDIPTSTQQDTEQKLSYE
                     ERKERIKILRKAEKILETCEQDIAKLEKELETIEQAMLQPENAANVSFVASYTQKKRA
                     LEQKYYEWELLAEDVETKKNELSN"
     gene            4686..4994
                     /locus_tag="PLD76_01385"
     CDS             4686..4994
                     /locus_tag="PLD76_01385"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOU26691.1"
                     /translation="MQFNNVSIQKKANVYFNGNVTSRTVLFPDGTRKTLGIMMPGEYE
                     FGTDSAELMEMLGGNMDVLLPNESEWKSYAEGDSFHVPAKSSFKLKVNTVVDYCCSYL
                     "
     gene            complement(5003..5815)
                     /locus_tag="PLD76_01390"
     CDS             complement(5003..5815)
                     /locus_tag="PLD76_01390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU26692.1"
                     /translation="MVQNKIVLITGASSGIGLVTAVQLAEQGYIVFASSRRGNSSVNL
                     PNLHFVQADVNDKDQMKNVIELIVKQYKKIDVVICNAGNGIAGAIEDCSSDEIRFQME
                     TNFYGVVNTIQQCLPYLREQKNGKILVISSLAAIFPIPYQAFYAASKAAVHILTQALS
                     IEVKRFNIQVCSILPGDTKTNFTKARSYAVQAQSPTSVYKEKMLASVAKMEQDEQNGM
                     KPDNVAKTIVKQVMAKKMGKVIVPGISNKILYGLAHILPTKLLLALLHKLYN"
     gene            complement(5875..>6873)
                     /locus_tag="PLD76_01395"
     CDS             complement(5875..>6873)
                     /locus_tag="PLD76_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HOU26693.1"
                     /translation="RKVNPNDQDTRVDFVDELGDTWEEYMIFHHKFVTWMEANGYPTA
                     KKYTSEEIEELVKKSPYFKATSADIDWMQKVHMQGRVQKWVDHSISVTINLPNDATEE
                     LVGNLYIEAWKSGCKGCTVYREGSRSGVLISANSEKKEKDPCNCYPEITPKRPKELEC
                     EIVRFQNNKEKWIAFVGLLDGRPYEIFTGLADDDDGILLPKNITSGKIIRNYNEETGV
                     KSYDFQFSNKRGYKTTIEGLSHKFNPEYWNYAKLISGVLRYGMPIDQVVRLVNGLQLN
                     NESINTWKNGVERALKKYIPDGTQAKGQVCPKCGDTLVFEEGCLICKKCGYSKCGS"
BASE COUNT         2240 a         1075 c         1223 g         2335 t
ORIGIN      
        1 acatttttct cagatatata atgtggcact caatttctga atcaattttt ttattttgtt
       61 gataaagcca gctgtagagt agaatttgta gtatatcttt tcgtctatct ttcaagtttc
      121 cattaaataa ttccccaatt gacgggtatt taagttctcc tgaacctgtt ttgtaatcaa
      181 tcaacatgat aactccatcc tttatttgaa ctctgtcaat agaacctttt atttttgcga
      241 atgttttata tttagatatg taatagtttt cttcgcattt gtattcatgg gttatgtgaa
      301 agaaaggagt tcgtttactt tctatatcta atagtttccg tacatattta ctaataactt
      361 gtgccactaa ataatgatta ccctctaata catgtggtgt tttatcttta aaataaaatt
      421 gtgaaaaccc ttcacttatt agtagctcaa tttttgatgt gtcacgtatc attgtgtcaa
      481 ttatttcctt tgaaatcatt ttatctttaa aatggacaaa taatttttcc ataacaaaat
      541 gaaatatgga cccaaatata ttcgactcaa tagattcggt aacactatct gtttgtttta
      601 tgttttcaat atatgaaaaa taaaattgta atggacaagt aatgaatgta ttaatgcttg
      661 aaggcgataa aacaatggca tgtaatttat ccattattgc gggtgtttta tcaatgcgaa
      721 tagcattagt tttagctaaa gatgtgttat aagtaagggt tttttctaca ataggaattc
      781 cgtaaccata gcgtagttga ttaataaaac ggcttttttc acccgtattc attatctcgg
      841 tacgtgtgtc ataaagtagg tatacagagg atgctctatg gaatagccga taaaaatgat
      901 aggcaaaaat ggcatcttga tgttcggtgg tgggcaaatc aaaaccctta cgtaagttat
      961 aaggaataaa tgagggcgat gattcgtttt taggaaaaat accctcgtta aatgaagtaa
     1021 ttattaagtt tttaaaatcc aaacaacgag tttcgagcat tcccataatt tgtaatccag
     1081 ccaacggttc tccttcaaac gagataacaa tagtactaag taatcgttta ataagcttgc
     1141 ttagtgtatc taatgataat ggaatgttcc attggttaag taaatcttgc atacgttgaa
     1201 gagcaataaa acattggtat agaaactctt gttccatcaa ttgattgttt tcatcagagt
     1261 tgtttatgga aactgatttt ctttctgcca gtaaatgccc aataatagct ataagataat
     1321 cacataatga ggctgtgtta caaggcttaa atagtaagca caataacgtg tctatttgta
     1381 aattgagagc tggtatacgt atccagttat tactatgtat cttctttata agctcttgat
     1441 agttagtatt gttttcgttg taaatatatc tatgtgataa taaagataaa acttgtttat
     1501 aatagaaaaa agtttttcct tcggtgtttg tattggctga tttttgtaag gctaataaat
     1561 tatcaattaa ttctttaata ggagtaaata ctaggggata gcccatggta atatttacct
     1621 tatctatgtg attaggtaat gaaaaaagca ttggaagaag aagattttca ttagaaagca
     1681 ctatggctgt ttctgtactt atattctcaa taccaagatg ttgcaaaatt tgatgaactt
     1741 gttttgcctg accaacactt gacggaatgg cgattgcttc aacgtattta tttccaataa
     1801 tttctttttc aatttttaat tgcgaaggaa atagcattaa attttcagct acataggtat
     1861 gagccttatt tgttttatct tgcacaaatt ctccctcgta gtcccaataa aaatcagcct
     1921 ttttttgttt ttttagttgt aagaataatg ttttttccac tttactcaaa gcattgaatc
     1981 caacaaatac tattttgtta taggaagcat gtatgccatt ttcagccaat tcgcgtagca
     2041 acataccttc atatgccaaa ttattctttt ttaaatcatc tttaaaggat tgatataaag
     2101 gaaacaatat ttcccatgta gccaaaaatt cttgttgtct tttaggttca ggattaaagt
     2161 tattccaaaa gcgttgtata attatttttt gttcgtttgt taaataatca ggcaaatagt
     2221 taatggattg tagttctttt acattttgaa atatatcttc agcatttact aaatatttat
     2281 caatatcatc aaaatcactt aacaaaattt ctccaaagga tgcaaatgaa tcaaacgttt
     2341 catgtttatt tgcaatggat tgatatatac catataatcg aaacaataaa cccattttat
     2401 cttcaagttg caacatagag tgcgaagtac aaaaatcgga aatgctagta attgtaggcg
     2461 aaaaaaaggt gtgttgggct gtttccatta ggtatttttc aaaaaatagt ccagaacgcc
     2521 tatttggaaa cacaaagcaa aaatcagtca gatcctcttt atatgtgtgg taaaatacag
     2581 aagcaacttg ttgtaaaaat ggtttcataa gtagtatact aaggatagca acaaaaatac
     2641 atataatatt gcgaaaaaaa cgattacaat gagattaaaa tttctgtatt gaaaaatacc
     2701 atttatcttt gcattatgat ttcaatagaa ggacttacag tagaatttgg tgcaaaagca
     2761 ttgtttgata acatatcata tgttgtaaac aaaaaagaca gaatagcatt ggttggtaaa
     2821 aatggtgctg gaaaaagtac aatgcttaaa attattgcag gcttacaaga gcctacacgt
     2881 ggtcagtgca ttattcctaa ggatttaacc atcggttatt taccacagca tatgattcac
     2941 agcgatgatg ccaccgtata tgaagaagct gaaaaggctt ttgcccatat tttggaaatt
     3001 caagatcaaa tagagaaaaa gaatatatta ctatgcgaac gagatgatta tgaatctgat
     3061 tcataccatt cgcttattga agagattaca catctcaacg aacaacttgc tatgttgggt
     3121 ggggtgaatt atgtggctga tattgaacga accttgtttg gtttaggatt tttacgcaca
     3181 gatttggcac gacctaccaa ggaatttagt ggtggttggc gtatgcgaat tgaattagca
     3241 aaattgttgc tccaacgtcc tgatgtatta ctgttagatg aaccaacgaa tcatttggat
     3301 atagaatcaa tacaatggtt agaaaatttt ttggcaagta gagccaatgc ggttatgtta
     3361 gtttcgcatg ataaagcctt tattaatgct gtaactacac gcacaattga aattagtttg
     3421 ggtaaaatat acgattatag ggctaattat tctacttacc ttgaacttcg caaagaacgt
     3481 agagaacaac aattgcgtgc gtatgaaaat caacagaagc aaatacaaga taccgaagaa
     3541 tttattgagc gttttcggta caaaacctct aaagctgttc aagtacaaag tagaattaaa
     3601 cagctcgata aaattgacag attagaggta gatgaagagg atacgagttc attacgtctc
     3661 aaatttccac ctgctccgcg ttctggaagt tttcctgtaa ttgctgaagg gcttgccaaa
     3721 ttatatggca attatgttgt gttctcaaat ctcgacctta ctattaaaag aggagaaaaa
     3781 gtggcatttg taggaaaaaa tggagaggga aagtcaactt tggtaaaatg tatcatgaat
     3841 gaaattgagt atgctggtaa gttgactctt gggcacaatg tgcaaatagg ttattttgct
     3901 caaaatcaag cgtctctgtt aaacgaatcg aaatctgttt ttgaaactat tgatgatgtg
     3961 gctgttgggg atattcgtac aaaaattcgg gatatattag gtgcatttat gtttggtggc
     4021 gaagaggctg ataaaaaagt aaaagtgctc tcaggaggag aacgcagtcg cttggcaatg
     4081 attagattat tgttgcatcc agttaatttg cttattttag atgagccaac aaaccattta
     4141 gatatacgtt caaaagatgt attaaaacaa gctatcaaag agtttgatgg aactgtgatt
     4201 gtggtttctc acgatagaga atttttagat ggtttggtaa cgaaggtgta tgaatttgct
     4261 gataaaaaaa ttattgaaca tataggcggt gtgtatgagt ttttacataa aaagaaactt
     4321 aataccgtgc aggaaattga gagaaaaaat ttgcccaaca aagatatccc cactagtaca
     4381 caacaggata cagaacaaaa attatcttat gaggaacgaa aagagcgtat aaaaatacta
     4441 aggaaagcgg aaaaaatact ggaaacttgc gaacaggata ttgcaaaact cgaaaaagag
     4501 ctggaaacaa tagaacaagc catgttgcaa cctgaaaatg cggctaatgt ttcttttgtt
     4561 gcatcctata cgcagaaaaa acgagctttg gagcaaaaat attatgagtg ggaattattg
     4621 gcagaagatg tggaaacaaa aaaaaatgaa ttatcaaatt aatataacta tcaaatccgt
     4681 ttattatgca atttaacaac gtaagtattc aaaaaaaagc caacgtatat tttaatggca
     4741 atgttactag ccgaacagta ttatttcctg atggtacacg taaaacccta ggtataatga
     4801 tgccaggaga gtatgagttt gggacagata gtgctgaatt aatggaaatg ttgggaggta
     4861 atatggatgt attactacct aacgaatcag aatggaaatc ttatgctgaa ggagattcgt
     4921 ttcacgtacc tgctaaatcg tcgttcaagt taaaagtgaa tacggttgta gattattgtt
     4981 gctcttattt gtaggttaca tgttagttat acaatttatg cagtagtgcc agcaatagtt
     5041 tggttggtaa tatatgagct aatccgtaca agattttatt cgaaatacct ggtacgatta
     5101 ctttccccat ttttttagcc attacttgtt ttacaatagt ttttgctaca ttatcgggtt
     5161 tcattccgtt ttgttcatct tgttccattt tggcaacaga ggcaagcatt ttttctttat
     5221 atacagaagt aggggattgg gcttgtacgg catacgaacg tgctttggtg aagtttgttt
     5281 tcgtatctcc aggcaagatg ctacatactt gaatgttaaa acgctttact tcaatcgaaa
     5341 gagcttgtgt tagtatgtgt acagctgctt tgcttgcagc gtagaatgcc tgatagggta
     5401 tggggaatat tgcagccaac gaagagataa ctagtatttt cccattcttt tgttcacgta
     5461 aataaggtaa acactgttga attgtattta ctacaccata gaaatttgtt tccatttgaa
     5521 aacgaatttc atcactacta cagtcctcga ttgcccctgc aattccattg ccagcattac
     5581 aaataacaac atcaattttt ttgtattgct ttacgattag ttcaataaca tttttcattt
     5641 gatccttgtc gttgacatcg gcttgcacaa aatgcaggtt aggaagatta acagatgagt
     5701 tgcctcttct agagcttgca aagactatat acccttgttc agcaagttga acagcggtaa
     5761 ctagtcctat acccgaacta gcacctgtaa ttaacactat tttattctgt accatacaag
     5821 aaattaacat gttagggcgg ttattacaca ccctaacaga gtagattaag tagattagct
     5881 accacatttt gagtaaccac attttttaca aatcaaacac ccttcttcaa aaactaaagt
     5941 gtcaccgcat tttgggcata cttgtccttt tgcttgggtt ccatcaggaa tgtatttttt
     6001 taatgctctt tcaacaccat ttttccaagt gttaattgat tcgttgttaa gttgcagacc
     6061 attaactaat ctaacaacct gatctatagg cataccataa cgtaatactc cagaaattag
     6121 tttggcataa ttccaatatt ctgggttaaa tttatgtgat aaaccttcga tagttgtttt
     6181 atatccacgt ttgttagaga attggaaatc ataactctta accccagttt cttcattgta
     6241 attacgaata atttttccac tcgtaatgtt tttaggtaat aaaatgccat catcatcgtc
     6301 agctagcccc gtgaaaattt cgtacggtct tccatctaat aatccaacaa aagcaatcca
     6361 tttttctttg ttgttttgga aacgaacaat ctcgcattcc aattctttag ggcgtttggg
     6421 tgttatttct ggataacaat tgcatggatc tttttctttt ttctcactgt tagcagagat
     6481 aagaacacca gagcgtgagc cttctctgta aaccgtacat cccttgcaac cacttttcca
     6541 tgcttcgata taaagatttc ctaccaattc ttctgttgca tcgttcggta aattaatggt
     6601 aacactaata gaatggtcta cccatttttg aactctacct tgcatatgta ctttttgcat
     6661 ccagtcaata tcggctgatg tagctttgaa ataaggtgat ttttttacta attcttcaat
     6721 ttcttcggat gtatactttt tggcagtagg atatccattt gcttccatcc atgttacaaa
     6781 tttgtggtgg aaaatcatgt attcttccca agtatcacca agttcatcta caaaatcaac
     6841 gcgtgtatct tgatcgtttg gatttacttt tct
//
LOCUS       DAOSMY010000032         6797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11257_length_6797_cov_4.165678, whole genome shotgun sequence.
ACCESSION   DAOSMY010000032 DAOSMY010000000
VERSION     DAOSMY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11257_length_6797_cov_4.165678"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2326)
                     /locus_tag="PLD76_01400"
     CDS             complement(<1..2326)
                     /locus_tag="PLD76_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU26694.1"
                     /translation="MKEFFTTKFRTWGEWIKQFIKQLVEWRKYRYHRFKNLVWYKKLA
                     NATATSFGLFLLLLLLIDINFLWLFGSSPSLYSISHPDQNIASEIISADKKVIGKYFT
                     ENRIPVTYNEISPILIKTLIATEDERFYEHFGIDFQGVMAAIKDMTKGNARGASTITQ
                     QLVKNMYKTRNQYSKGLFGYIPFVKLLIMKAKEWITAVKIEISYSKEEILTMYFNTVD
                     FGSNAFGIHTAAKTYFKTTPDKLNYEQSATLVGLLKATTTYNPRLNPKKSTERRNVVL
                     QNLVSQGILTQIETDSLKAIPMKLDYSVEQSYDGKALHFRAFLSKYLEPWEKQTGYDI
                     YADGLKIYVTIDSRLQQYAEESVQKQMRTVQRRFNSHWGNENPWQDENRVEIPDFVEK
                     IARKTTTYSNLHKKYKGNKDSIDVYLNLKKKTKVFDYTYGEKDTLFSVVDSIRYMSKF
                     MHCSFMAMEPTSGEVKAWVGDINFPFWQYDKVGQSKRQPGSTFKLFVYTAAMMNGYTP
                     CDKLTDQQTIWNYVENGEPKTWIPRNAEVRYTGYNMTLKNAFARSINTIAVQLAQKIG
                     IPEVIKYAHLMGIKTPLENMPSTSLGSSDVSLLELVNAYATVINEGKYHEPILVTRIE
                     DRHGRVIYQANRTQKQVIPYETAFLMTEMLKGGMTERGGTTQALWEWDLFHYNTDFGG
                     KTGTSSNYSDAWFVGVTQNLIGGAWVGGEHRSIHFRTGNYGEGSRTALPVFALFMEKV
                     FKDPNFKHYQGKFPTKPKVKINKPYNCQSFVQPDS"
     gene            3017..5146
                     /gene="pnp"
                     /locus_tag="PLD76_01405"
     CDS             3017..5146
                     /gene="pnp"
                     /locus_tag="PLD76_01405"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU26695.1"
                     /translation="MMNVITKTITLGDGRTISIETGKLAKQADGSVMVRMGNTMLLAT
                     VCAAANAVPGTDFMPLSVDYKEKFAANGRFPGGFTRREGRASDYEILVSRLVDRALRP
                     LFPDDFHAETFVNVTLFSADGEDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDG
                     QFVVNPTFSALKNADMDIMVAATMDNIMMVEGEMKEVSEDDLLQAMKVAHEAIKVHCQ
                     VQLDLMEAAGKTQKRVYNHEDNDEELKKDIWAKTYDAVYAVASACNPDKHSRIANFEA
                     VKETYIASLTPEVAAEKKGLISKYYHDVEKEAMRRAILDEGKRLDGRKTTEIRSIWSE
                     IDYLPGAHGSAIFTRGETQSLTTVTLGTKQDEKLIDEALTQGKERFLLHYNFPPFSTG
                     EARASRGTGRREIGHGNLALRALKGMIPDNYPYVVRVVSEILESNGSSSMATVCAGSL
                     ALMDAGVQIKKPVSGIAMGLISENKGKNFAVLSDILGDEDHLGDMDFKVTGTRDGITA
                     TQMDIKVDGLSYEILEKALSQAKEGRMHILNKIEETIATPRPDFKANAPRIVTLQVPK
                     DMIGAIIGPGGKIIQAIQLESGASVSIEEKENVGVVEIAANNKQAIDIALAKVKGIVA
                     VPEVGEVYKAKVKSIMPYGAFVEFMPGKEGLLHISEISWERIEDMQLTGIKENDEIEV
                     KLLEVDKKTGKFKLSHKVLTQRPPRKE"
     gene            5222..5560
                     /locus_tag="PLD76_01410"
     CDS             5222..5560
                     /locus_tag="PLD76_01410"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HOU26696.1"
                     /translation="MKAVDYFHKKPALLNCAQAILTAWQKEYDIAENTILEFKQYGGG
                     RAPEGACGALYAAEYLMKKHHQQSIYKEFSVYAGSIACKEIRKNGVVSCQKCVEIADE
                     LLQNARVKKE"
     gene            complement(5625..>6797)
                     /locus_tag="PLD76_01415"
     CDS             complement(5625..>6797)
                     /locus_tag="PLD76_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU26697.1"
                     /translation="VTIVIHKFMRRFLTVFAAFIIMLCLGSVYAWSVIASELMSVYHF
                     SAAQSQIIFGTIIAIFPITMIFVGQLARKLHPRYLGYISGVLFLSGYLLAAHANGHFF
                     LTYLGLGITAGIATGFGYWVSLTIPVQWFPEKKGLITGVAAAGFGLGAVFISAIAEKM
                     LIAGKDVLEVVQFIGIVYGLTVILFSNLITPNSDLSKSGVVAIRIKECISSTIFLKLF
                     SGIFLGTFAGLLIIGSLKIIGEQSNINNHILILGVSVFATSNFLGRLIWGFLSDYIGA
                     SLSIFFALLIQSLAILSLNLFSLSDHTYLLLSFLIGFGFGGNFVLFAKEAAQLYGVQK
                     LGLIYPYVLLGYAIAGIAGPAVGGILFDFSDSFFYAILVASIMSLIGSVLFFKTIH"
BASE COUNT         2230 a         1146 c         1259 g         2162 t
ORIGIN      
        1 atgagtctgg ttgtacaaat gattggcaat tataaggctt gttaatttta acttttggct
       61 ttgtagggaa cttgccttga tagtgtttaa aattgggatc tttaaatact ttttccataa
      121 acaaagcaaa cactggcaaa gctgtgcgac ttccttctcc gtaatttcca gtacggaaat
      181 gaatacttct atgttcgcca cctacccatg cacctccaat caaattttgc gttacaccta
      241 caaaccacgc atctgaatag ttagatgaag tgccagtttt tccaccaaaa tcagtgttat
      301 agtgaaataa atcccactcc catagggctt gtgtcgttcc tcctctttct gtcataccac
      361 cttttagcat ttctgtcatc aaaaaagctg tttcgtaggg gataacctgt ttttgtgtac
      421 gattagcttg gtaaattact cttccatggc ggtcttctat gcgtgttact aaaatgggtt
      481 cgtggtattt cccttcatta ataactgtgg catatgcatt tacaagttca agtagagata
      541 catctgatga acctaatgag gtagatggca tattttctaa aggggttttt attcccatta
      601 gatgtgcgta tttaatcact tctggaatgc caattttctg agctagttgt acggctatag
      661 tattgattga acgagcaaac gcatttttca aagtcatgtt gtatccagta tacctaactt
      721 ctgcattgcg aggaatccat gttttgggtt ctccattttc tacataattc caaatggttt
      781 gctgatcggt taatttgtca caaggagtgt agccattcat catagcagcg gtataaacaa
      841 ataatttaaa cgttgatcct ggttgtcgtt ttgattgtcc tactttatcg tattgccaaa
      901 atgggaagtt gatgtctcca acccatgctt taacttcacc actagtgggc tccatagcca
      961 taaaactaca atgcataaat ttactcatat agcgtattga atccacaaca ctaaataatg
     1021 tgtctttttc tccatatgta tagtcgaata ctttcgtttt cttctttagg tttaaatata
     1081 catcgatgga gtctttattc cccttatact ttttatgcag attgctgtat gtagttgttt
     1141 tacgtgcaat cttttctaca aagtctggta tttctactct attttcatct tgccaagggt
     1201 tttcgttgcc ccaatggcta ttaaaacgcc gttgcactgt tcgcatttgc ttttgaacgg
     1261 attcttctgc atattgttgc agacgcgaat caatggtaac atatattttt aagccatctg
     1321 catagatgtc atagcccgtt tgtttttccc acggttctaa atatttagat aaaaaagctc
     1381 taaaatgcaa ggctttccca tcgtatgatt gttctacgct ataatcaagt ttcataggaa
     1441 tagcctttaa tgaatctgtt tctatttgtg taaggatacc ttgcgaaaca agattttgta
     1501 ataccacatt tctcctttca gttgattttt taggatttaa tcgaggatta taggttgtag
     1561 ttgcttttaa taatccaact aaagtagctg attgttcgta attgagtttg tctggagtcg
     1621 ttttaaaata cgtttttgca gccgtatgga ttccaaaagc attacttcca aaatcaaccg
     1681 tattaaaata cattgttagt atctcttctt tcgaataaga aatttctatt ttaaccgcag
     1741 tgatccattc tttggctttc attatcagta atttaacaaa tggaatataa ccgaataatc
     1801 cttttgaata ttgattacgc gttttataca tgttttttac tagttgttgt gtaatggtac
     1861 tagctcctcg tgcgtttcct tttgtcatat cttttatagc agccattact ccctgaaaat
     1921 caatgccaaa atgctcataa aagcgttcat cttccgtagc aataagcgtt tttataagaa
     1981 taggagaaat ttcattatag gtaacgggaa tgcgattttc ggtaaaatat ttgccgatta
     2041 cttttttgtc agcagaaata atttctgaag caatgttttg gtctgggtgg cttatgctat
     2101 acaaacttgg agaagaccca aacaaccaca aaaaattgat atcaattaat aagagtaata
     2161 atagaaaaag accgaaagat gttgctgttg cattggctaa ttttttatac caaactagat
     2221 ttttaaaacg atgataacga tacttacgcc attcaaccaa ttgtttaata aattgtttaa
     2281 tccattctcc ccaagtacga aattttgttg taaaaaattc tttcatagta tataaaaaag
     2341 cactattggt ttcaatatga atataggtgt taagtaggaa aaacttactt catattgtgg
     2401 cgtcaaagtt atttaaaaga atgtttctgt caaaatttta ttgccgatat atttgtcttg
     2461 ttcttaaata tgtaaaacaa gtattcagaa gaattgaggg ggttgtagta attgtttgat
     2521 agttaatgtt ttctggtttg tagaaataaa aaagttagca ttacgctaac aaaaaaaccg
     2581 taccgctatt tacgagtcaa acttcgatat ctaaagtttt gagtgctcaa tttcctttgt
     2641 aatctgccta cttgtttcca atagcagcac gccattagaa attgaaaata gtctcagtat
     2701 gtaaaagagt gttaagtttc accaaatgcg atacggtact tcaaagatac actctttttg
     2761 tttattatcc aaatttttca cacaaaacta tgcttgtatg ttgtaaaata tagtaaccaa
     2821 tgtgtctgaa gatataattc aaagagacca ataacacatg aatgagtaaa tgttgaaaaa
     2881 tattctgttt gattaaaaac aacttttttt gacccatatg caacacaaat ccttaaaaat
     2941 aaactcattt tcaaggatta aaatgtatct ttgtgtcgaa attttaagaa tatattaaca
     3001 acatgtaatt tttagtatga tgaatgtaat tacaaaaact atcacactcg gtgatggaag
     3061 aacaattagc attgaaaccg gaaaattggc aaagcaagcc gatggttcag tgatggtacg
     3121 catgggtaat accatgttat tggcaacagt ttgtgctgcc gcaaatgcag ttcctggaac
     3181 tgattttatg cccctttctg ttgattataa agaaaagttt gctgcaaacg gtcgtttccc
     3241 tggcggtttt acacgtcgtg aaggtcgtgc ctctgattat gaaatacttg tttcccgatt
     3301 ggttgacagg gctttacgtc cattatttcc agatgatttt catgcagaaa cgtttgttaa
     3361 tgtaaccctt ttttctgctg atggagaaga tatgcctgat gcacttgctg gtttagccgc
     3421 ttcggctgct ttagcagtat ctgatattcc ttttaatgga cccatttctg aagtacgtgt
     3481 agctcgtatt gatggtcagt ttgtagtaaa tcctacattt tctgcattga aaaatgccga
     3541 tatggatatt atggttgccg ctactatgga taatatcatg atggtagaag gtgaaatgaa
     3601 agaagtatct gaagatgatt tattgcaagc aatgaaagtt gctcacgaag ctattaaagt
     3661 acattgtcaa gtacaacttg acttgatgga agctgctgga aaaacacaaa agcgtgtgta
     3721 taaccacgaa gataatgatg aagaattgaa aaaagatatt tgggcaaaaa cctatgatgc
     3781 agtgtatgca gtagctagtg catgtaatcc agataaacat tctcgtattg ctaattttga
     3841 agcagtaaag gaaacgtata tagctagttt aactccagaa gtagccgctg aaaagaaagg
     3901 tttgataagc aaatattacc atgatgtaga aaaagaagct atgcgtcgtg ctattcttga
     3961 tgagggtaag cgtttggacg gaagaaaaac aacagaaatt cgttctattt ggtcagaaat
     4021 agactattta ccaggagctc atggttcagc aatttttact cgtggcgaaa ctcaatcact
     4081 tacaacagta actcttggga caaaacaaga tgaaaaatta attgacgaag ccttaactca
     4141 aggcaaagaa cgttttctat tgcattataa cttccctcca ttctctactg gtgaagcaag
     4201 agcatctcgt ggtacaggaa gacgtgaaat tggtcatgga aatctagctt taagagcatt
     4261 aaaaggcatg attccagata attatccata tgtagtacgt gtagtatcag aaatcttaga
     4321 atcaaacggt tcttcgtcta tggctacagt atgtgcaggt tcgttggcat tgatggatgc
     4381 aggtgtgcaa attaaaaaac cagtatctgg tattgctatg ggtttgattt ctgaaaataa
     4441 aggaaaaaac tttgctgttc tttctgatat tttaggagat gaagatcatt tgggtgatat
     4501 ggactttaag gtaactggta cacgcgatgg tattactgca acacaaatgg acattaaagt
     4561 ggatggtttg tcgtacgaaa ttttagagaa agctctttcg caagccaaag aaggcagaat
     4621 gcacatctta aataaaattg aagaaactat tgctactcca cgtcctgact ttaaggcaaa
     4681 tgctcctcgt attgtaacat tacaagtacc taaagatatg attggtgcaa ttataggacc
     4741 tggtggaaaa ataattcaag caattcaatt agaatctgga gcttcagtat ctatcgaaga
     4801 aaaggaaaat gttggtgttg tagaaattgc agctaataat aaacaagcaa ttgatatcgc
     4861 tttagctaaa gtaaagggta tagttgcagt tccagaggta ggtgaagtgt acaaagcaaa
     4921 ggtaaaatct attatgcctt atggtgcatt tgttgaattt atgcctggca aagaaggttt
     4981 gcttcatatt tctgagatta gttgggagcg tatcgaagat atgcagttaa ctggaataaa
     5041 agaaaatgat gagattgagg ttaaattact tgaagtagat aaaaaaacag gtaaatttaa
     5101 attatcgcat aaagtattga cacaaagacc tcctcgaaaa gaatagtata ttgttcaatc
     5161 aaaatcaaag caccacttct tttcaggatg tggtgctttt tttaaataaa agataaaaga
     5221 aatgaaagct gttgattatt ttcataaaaa gccagcattg cttaattgtg cacaagccat
     5281 attaactgcg tggcaaaaag agtatgacat tgcagagaat acaatcctag aatttaaaca
     5341 atatggaggc ggacgtgctc ctgagggtgc ttgtggggca ttgtatgctg ctgagtattt
     5401 aatgaaaaaa catcatcaac aatcaattta caaggagttt tcagtatatg caggctctat
     5461 tgcatgcaaa gaaattagaa aaaatggagt agtgagttgc caaaaatgtg tagaaattgc
     5521 agatgaattg ttgcaaaatg cccgtgtcaa aaaagaataa acagcactct cgatttttgt
     5581 agcaacgatt acactagttt tttgctaatg tatttcttct cgcgttaatg tattgtttta
     5641 aaaaagagca cactgcctat taagctcatg atacttgcta ctaaaatagc ataaaaaaat
     5701 gaatctgaaa aatcaaacag aatgccacca accgcaggtc cagctatacc agcaatggca
     5761 tatcctagca aaacataagg gtatatcaac ccaagttttt gaacaccata tagttgagct
     5821 gcttctttgg caaatagtac aaaattacct ccaaatccaa aacctattaa aaatgacaat
     5881 aacagatagg tgtggtctga taaagaaaaa agattgaggg ataaaatagc caatgattgt
     5941 atgagcaatg caaaaaaaat actaagactt gccccgatat aatcacttaa gaatccccaa
     6001 ataagacgcc ccaagaagtt ggatgttgca aatacggata caccaagtat tagtatgtgg
     6061 ttgttgatgt tgctttgttc gccaataatt tttaaactcc caataataag cagaccagca
     6121 aatgttccta aaaaaatacc gctaaatagt ttgaggaaaa tcgttgatga tatacattct
     6181 tttattcgga tagcaaccac tcccgactta gataaatcac tatttggagt aattagattc
     6241 gaaaatagaa taacggttaa gccgtataca ataccaataa attgcactac ctctagcaca
     6301 tctttccctg ctatcaacat tttttcggca atagcagaga taaatacagc ccccaatcca
     6361 aaaccagccg cggctacgcc tgtaattaag cccttttttt caggaaacca ttgaacaggg
     6421 atggttagag aaacccagta tccaaaacct gttgcaattc ctgcggtgat ccctagccca
     6481 agataagtaa ggaaaaaatg accattggca tgagcggcta ataggtatcc gctcaaaaaa
     6541 agaactccag aaatatatcc caaataccgt gggtgtagtt tacgagccaa ttgcccaaca
     6601 aaaatcatag tgataggaaa tatggcaatt atggtaccga agataatttg tgattgagct
     6661 gccgagaaat gatacacaga cattaattcg gatgcaatta cactccatgc atatacactc
     6721 cctaaacaaa gcatgatgat aaatgcggca aatacggtta aaaatcttct cataaattta
     6781 tggattacaa tagttac
//
LOCUS       DAOSMY010000033         6769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11328_length_6769_cov_3.970211, whole genome shotgun sequence.
ACCESSION   DAOSMY010000033 DAOSMY010000000
VERSION     DAOSMY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11328_length_6769_cov_3.970211"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..758)
                     /locus_tag="PLD76_01420"
     CDS             complement(168..758)
                     /locus_tag="PLD76_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOU26698.1"
                     /translation="MIAIGDTIVSFDVINEQFLCDIASCKGACCIEGDCGAPLTLDEV
                     GILEEILPVVWNDLSEAAKKVIDKQGVAYADIEGEMVTSIVGNKDCVFTYQEDGVCKC
                     AIEKAYNEGKTTFKKPISCHLYPIRIQKYKQFTAVNYHKWDICSAACVLGKRQKLRMY
                     QFLKEPLIRRFGSDWYNELVVVASELDKQGVKLRRK"
     gene            complement(755..1240)
                     /locus_tag="PLD76_01425"
     CDS             complement(755..1240)
                     /locus_tag="PLD76_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOU26699.1"
                     /translation="MIDIKELSLLAKKAEASSKHLFRKHKKIVEKLDDHVAVLHEKYT
                     AQYDCLACGNCCRSLGPFLTDRDIDKIAKALHLKPSAVVEKYIRIDEDGDYVFKTMPC
                     PFLMPDNYCQIYENRPKACKEYPHTDRKKFYQITNLSIKNSHVCPIVYEVLEDLKKEL
                     K"
     gene            complement(1243..1779)
                     /locus_tag="PLD76_01430"
     CDS             complement(1243..1779)
                     /locus_tag="PLD76_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU26700.1"
                     /translation="MKKLVFIFVSALFYSVSIVAQDDSVLEYVHLFPNCVDNEESTIY
                     GGTYGEYPPEIEDAIFPGGGFVKMSQYIYTNTEMQPAYTGEINENGDSLLVTGIVRVE
                     FVVDRCGKVGRVRVVESVSELQDAEALRIVEGFPIFKPASLKDYRVKIAYIAPVYFSK
                     KVMPRKAPEIDYDSYYYW"
     gene            complement(1812..3032)
                     /locus_tag="PLD76_01435"
     CDS             complement(1812..3032)
                     /locus_tag="PLD76_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU26701.1"
                     /translation="MNFPLYIAKRYLFSKKSHNAINIISLISASGVAVGTVALVCVLS
                     VFNGFQGVIEGLFGKFDAQLQVTVNEGKSFNPQISSVQSLYKMPEIKVICEVVQDNAL
                     LIFSDKQTPAVIKGVEENYTQLNAMDEILLDGEFKLYDGVFDMGVAGVGLAQTLGCGV
                     RFVEPLQLFAPKRNTTVSLARPDNAFTSEFVFLSGIFMVQQAKYDANLLFVRLPLARK
                     LFGYDNEVTSLELRLTDGANELQVQKKIQTVLGDTFSVKNKYEQQADFFRMMEVEKWI
                     TYLILSFIVLIAIFNVVGSLSMLIIEKKNDTKVLMNLGANRKLIFKIFLFEGWLISIL
                     GVVVGILLGLVVCWIQIQFGILSLNGTDGAFVVEAYPVLVKWTDIVLIFGTVSLLGFL
                     AAIYPAHKLTREVK"
     gene            complement(3042..3377)
                     /gene="rbfA"
                     /locus_tag="PLD76_01440"
     CDS             complement(3042..3377)
                     /gene="rbfA"
                     /locus_tag="PLD76_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU26702.1"
                     /translation="METKRQNKIARLIQKEISEILQSITKEMNQGVLITPTIVRVSPD
                     LGVAKIYLSIFPSEKSADMLAFVTERSQTIKHHLAGRTRFQLRTVPSLSFYLDDSLDY
                     IEHIDKLLK"
     gene            complement(3426..4064)
                     /locus_tag="PLD76_01445"
     CDS             complement(3426..4064)
                     /locus_tag="PLD76_01445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU26703.1"
                     /translation="MNISSQLDEYILQHSEQEPLLLQELYREAHVRLLRPRMVSGHLQ
                     GRILSMLSKMIQPKRILEIGTFAGYSALCLAEGLPADGKIITIEIDDEMEDFARSFID
                     KSPYKEQIELVIGDSLHHIPTMDEKFDLVFIDADKRDYCAYYDVVFDKVLSGGYIFAD
                     NTLWDGKVVEPLVPNDKQTAGVLAFNRYIAADTRVEKVVLPLRDGLTLIRKK"
     gene            complement(4071..5291)
                     /locus_tag="PLD76_01450"
     CDS             complement(4071..5291)
                     /locus_tag="PLD76_01450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU26704.1"
                     /translation="MNIQTHLFANNIRLLHVHDNSEVAYAGVIINTGTRDELPNEEGY
                     AHLTEHMLFKGTQKRNSTQIINRIEDVGGELNAYTSKEETVVYSVFLTKYLNSAVDVI
                     VDIVFNSIIAQHELQKEVDVVIDEIHSYMDSPSELIVDDFENLLFNQYAIGRNILGTE
                     ESLQKCTPESLKEFINRTYTTDEMVFFTLGSFSIEKVIRTLGKYIASIPATKRTYARE
                     TPINYVPTRVTLEKKTLQAHYILGNRAYSMQEEKRFAFHLLNNIVGGQGMNSRLNMSL
                     REKSGLVYTIESHYTPYSDTGLWTVYFGTDHTDVPQALRLIYKEIKKVKENQLSPIRL
                     FKAKQQLIAQMLIAGENKENWIIAKAKHFLNFNSLMEQTEIEAKIMAISSSQLCDVAN
                     ECLQEEQFTHLHYM"
     gene            complement(5373..>6769)
                     /locus_tag="PLD76_01455"
     CDS             complement(5373..>6769)
                     /locus_tag="PLD76_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU26705.1"
                     /translation="PGMVLIEGGTFTMGRVTEDTYSEWNNYPRRVTVSTFYMDQHEVS
                     NLDWREYVYWMRLMFSASPKLVNRALPDTLVWRDELAYNEPYLEYYFSHVAYQEYPVV
                     GVSWEQAVDYCLWRTDRVNELRMVQAGVIELPDFKALHKDSATVEKQDSFARKQLFNV
                     DKYLKNSDYKPDKEKSKVKTVFGDARKTNMSDGILLPQYRLPTEAEWEFAAYGIKSEE
                     GMENYDERRIFPWDGSQLRNPTKKHRGKMMANFVRGRGDYMGVAGDLNDKASITAPVS
                     SFWPNDYGLFNMAGNVNEWVMDVYRPMTSENVQEYNPFRGNMYVSPIFNDSISDAGTT
                     RIVKLDSLGRAVKAIAVSKDSISDYVKYDVRNYEDGDAKTSVDANQWKNDIDPDESTK
                     RLYNPDTDAEGMLATHISNTTRVFKGGGWKDRAYWLNPATRRYLEQTKSRSDLGFRCA
                     MSRVGSDKGNQDKK"
BASE COUNT         2055 a         1446 c          999 g         2269 t
ORIGIN      
        1 ccgcgaccct aaccttggca aggttatgct ctaccaactg agctattttc gcatttttgt
       61 tttatcggaa tcgcatttcc aatagttgct tttgtaattg agcgtgcaaa tatactgcca
      121 ttttttgttt tgtgcaacat cgaaacaaaa aaaatgccac tttttgctta ttttctacgt
      181 aatttcaccc cttgtttatc gagttctgaa gctactacta ccaattcgtt gtaccaatcg
      241 ctaccaaaac ggcgtataag cggttctttt aagaattgat acatacgcaa tttctgtctc
      301 ttgcccaata cacaagcggc actgcatata tcccatttat ggtaattaac ggcggtaaat
      361 tgcttgtact tttgtatgcg aatgggatat aaatgacacg atatgggctt tttaaaagtt
      421 gttttgcctt cgttataagc tttttcgatg gcacacttac ataccccatc ttcttggtag
      481 gtaaatacgc agtctttgtt gcctacaata gatgttacca tttcgccttc tatgtcagca
      541 tacgctactc cttgtttgtc aattactttt ttggctgctt ctgataaatc gttccacacc
      601 acaggcaata tttcctctaa gatacctact tcgtcgagag taaggggtgc accgcaatca
      661 ccttcgatac aacaagctcc tttgcaagag gctatatcgc acaaaaattg ttcgtttatg
      721 acatcaaaac taacaatggt atctcctatc gctatcattt taattctttc tttaagtctt
      781 ccaacacttc gtacacaatg gggcaaacgt ggctattttt aatactaaga ttggttattt
      841 ggtaaaactt ttttctgtca gtatgtggat attccttaca agctttgggt ctgttttcgt
      901 aaatctgaca ataattatct ggcatcaaaa atggacaagg catggtttta aacacatagt
      961 cgccatcttc atcaatacgg atatactttt ctaccactgc cgatggtttt aagtgcaagg
     1021 ctttggctat cttgtcaata tccctatccg taagaaaagg tcctaaacta cgacagcaat
     1081 taccacaagc taaacaatcg tactgtgcag tatatttttc gtgcaaaact gccacatgat
     1141 catcaagttt ttctacaatc tttttgtgtt ttctaaaaag atgtttcgac gaagcttcgg
     1201 ctttttttgc caataaggat aattctttta tatcaatcat gcttaccaat aataataact
     1261 atcataatct atttcaggag ctttgcgtgg cattactttc ttgctaaaat acacaggggc
     1321 tatatacgca attttaactc ggtaatcttt taaacttgca ggcttaaaaa tcggaaaacc
     1381 ttcgactatt cgcaaagctt ctgcatcttg cagttcggat acagactcta ccacacgcac
     1441 acgccctacc ttaccacacc tatctactac aaattctact ctcacgatac ccgtaaccaa
     1501 cagggagtct ccattttcgt ttatttcgcc ggtatatgca ggttgcattt cggtgtttgt
     1561 ataaatatac tgcgacatct ttacaaaccc acctcctgga aaaatagcgt cttctatttc
     1621 aggaggatac tcgccatacg taccaccata aatggtagat tcttcattat ctacacaatt
     1681 ggggaataaa tgtacatact ccaacacaga atcgtcttgt gctactattg agacagaata
     1741 gaatagtgca ctaacaaaaa taaaaacaag ctttttcata ttttatactc atgtttacac
     1801 atattaatcc tttattttac ttctcttgtc aacttatgtg caggataaat ggcagccaaa
     1861 aaacctaaaa ggctaactgt accaaatatt aacacgatat ctgtccactt taccaacaca
     1921 ggatatgctt ctacaacaaa tgctccatct gtaccattca acgataagat gccaaactgt
     1981 atctgaatcc aacatacaac taatccgagt agaataccca caactacccc aagtatagat
     2041 ataagccatc cttcaaataa aaatatttta aaaatcaact ttcgatttgc tcctaaattc
     2101 attaatactt tggtatcatt tttcttttcg ataattaaca tcgagagaga cccaactaca
     2161 ttaaaaatag ctattagcac aataaaagat aaaattaaat aggtaatcca tttttctact
     2221 tccatcattc tgaaaaaatc agcttgctgc tcgtatttat tttttactga aaaagtatca
     2281 cccaatacag tttgtatttt tttttgtact tgtaactcat ttgcaccatc agttagccta
     2341 agttctaaag aggtaacctc attatcataa ccgaataatt tacgtgccaa tggcaacctt
     2401 acaaacaata aattagcatc atatttggct tgttgcacca taaaaatacc agataaaaaa
     2461 acaaattcag acgtaaacgc attatctggt cgagctaagc tgacagtagt atttcgtttg
     2521 ggggcaaaca actgtaatgg ttctacaaaa cgcactccac aacccaaagt ttgtgctaat
     2581 ccaacacccg ccacgcccat atcaaatacg ccatcataca atttaaactc accatctaac
     2641 aggatttcat ccatagcatt gagttgagtg taattttctt ccaccccttt gattacagct
     2701 ggtgtttgtt tatcagaaaa aatgagcaag gcattatcct gaaccacctc acatattact
     2761 tttatttcgg gcatcttgta caaagattgt acagaagaaa tttgtggatt gaaacttttc
     2821 ccctcattca cggtaacttg caactgtgca tcaaacttac caaacaatcc ttctatcact
     2881 ccttgaaatc cgttaaaaac agacaataca cagaccaatg ccacagtacc tacagctact
     2941 ccacttgctg aaataagcga tataatatta atggcattgt gcgatttttt tgaaaacaaa
     3001 tacctcttag ctatgtataa aggaaaattc acggctattc cttattttag aagtttatcg
     3061 atatgttcga tatagtcaag cgaatcatca aggtaaaaag ataaactagg tacagtacgt
     3121 aactgaaacc gagtacgacc agccaaatga tgtttaatgg tttggcttct ttctgtaaca
     3181 aatgctaaca tatcggcact tttttcagaa ggaaatatac tcagataaat ttttgctacg
     3241 cccaaatctg gactcacacg cacaatagtt ggagtaatca ataccccttg attcatctct
     3301 ttcgtaatcg actgcaaaat ttcacttatt tccttttgaa tcagtcgagc tattttattt
     3361 tgtctttttg tttccataca tgttattact ttattctatg caaatatacg atatttttct
     3421 ggaagttatt tttttcggat taaggttaat ccatcacgca agggcaatac cactttttct
     3481 acacgcgtat ctgctgcaat atacctatta aatgctaata cgcctgctgt ttgtttatca
     3541 ttgggaacta aaggttctac aaccttacca tcccacaatg tgttatctgc aaaaatataa
     3601 ccgccagata gcactttatc aaatacaaca tcgtaataag cacaataatc acgtttatca
     3661 gcatcaataa atacaagatc aaatttctcg tccatggttg gtatatggtg caacgaatcg
     3721 ccaattacca attctatctg ttctttatat ggagatttat caataaaaga acgagcaaaa
     3781 tcctccattt catcatcaat ttctatggta ataatttttc catcagcagg tagcccttct
     3841 gccaaacaca aagcagaata tccggcaaat gtaccaattt caagaatacg ttttggttgt
     3901 atcattttag acagcatact taatatacgt ccttgtaaat gtccagaaac catcctagga
     3961 cgcaacaaac gcacatgggc ttctctgtac aattcttgca acaacaacgg ctcttgctcc
     4021 gaatgttgca atatatactc atctaattga cttgatatat tcatttttag ctacatataa
     4081 tgcaaatggg taaattgttc ttcttgcaaa cattcattgg caacgtcaca aagttgagat
     4141 gaactaattg ccataatctt ggcttctatc tctgtttgtt ccatcaaact attaaaattt
     4201 aaaaaatgtt ttgcctttgc tataatccag ttttctttat tttctccagc aataagcatt
     4261 tgggctatta attgctgttt agctttaaat aggcggatag gagacaattg attctctttt
     4321 acctttttta tttctttata tataagtcgt aatgcttggg gcacatctgt atgatctgtt
     4381 ccaaaatata ctgtccataa gccagtatct gaataaggcg tgtaatgaga ttctatggta
     4441 tacaccaagc cacttttttc tcgcaacgac atattcaatc ggctattcat tccttgccct
     4501 cctactatgt tatttaacaa atgaaaggca aatcgttttt cttcttgcat agaataagct
     4561 ctattaccta gtatataatg agcttgaagt gttttttttt ctaatgtaac ccttgtcgga
     4621 acatagttga ttggggtttc tctagcatag gttcgcttag tagcaggtat gcttgctata
     4681 tacttgccga gtgtacgaat aactttctcg atagaaaaac taccaagcgt aaaaaacacc
     4741 atttcatctg ttgtgtatgt acgattgata aattctttta acgactctgg cgtacatttc
     4801 tgtaaacttt cttcagttcc caatatattt cttcctatag catattgatt aaatagtaaa
     4861 ttctcaaaat cgtctacaat caattcagaa ggactatcca tataagaatg aatctcatct
     4921 attacaacat ctacttcttt ttgcaattca tgttgagcta tgatggaatt aaatacaata
     4981 tctacaatta catctacagc tgaattgaga tatttagtaa ggaataccga ataaacaacg
     5041 gtttcttctt tactagtata agcattcagc tcaccaccca catcttctat ccggtttatt
     5101 atttgcgtgc tatttctttt ctgcgttcct ttaaacagca tatgttcagt aagatgagca
     5161 tagccttctt catttggcaa ctcatcacgt gttcccgtat taataataac acctgcatat
     5221 gccacctcag aattgtcgtg cacgtgcaac aagcgtatat tatttgcgaa aagatgcgtt
     5281 tgtatattca tatagatagt ataatttacg tcaaaaaagg atagcttatc tagctatcct
     5341 tttctatatt ttttcaataa actttctaca gcttattttt tatcttgatt tcctttgtca
     5401 gaacctacac ggctcatagc acaacggaaa cctaaatcgc tacgactttt tgtttgttcc
     5461 aaataacgcc ttgttgcagg atttaaccaa tacgctctat ctttccaacc accaccttta
     5521 aatacacgag ttgtattcga gatatgagta gctaacattc cttctgcatc ggtatcagga
     5581 ttatataaac gtttggttga ttcgtctggg tcaatatcat ttttccattg gtttgcatca
     5641 acacttgttt ttgcatcacc atcttcatag ttacgtacat catatttcac gtaatcagaa
     5701 attgaatcct tacttactgc tatggctttt acagctcttc ctaagctatc aagttttaca
     5761 attcttgttg ttcctgcatc agaaattgaa tcattaaaaa taggtgaaac atacatgtta
     5821 ccacggaaag gattatattc ttgaacgttc tcgctagtca ttggacgata tacatccatt
     5881 acccactcgt ttacgttacc cgccatattg aacaaaccat aatcgttggg ccaaaatgaa
     5941 ctcactggag cagtaatcga tgctttatca ttcaaatcac ctgcaacacc catgtagtca
     6001 ccacgacccc ttacaaagtt tgccatcatt tttcctctat gtttttttgt tgggtttctt
     6061 agctgagagc catcccatgg gaatatgcgt ctttcgtcat agttttccat tccttcttct
     6121 gatttgatac cataagcagc aaactcccat tctgcctcag ttggcaggcg gtattgtggc
     6181 aacaaaattc catcagacat atttgtttta cgagcatctc caaacactgt ttttactttg
     6241 ctcttttctt tgtctggttt ataatcagaa ttttttaggt atttatccac attaaatagt
     6301 tgttttctag caaaactatc ttgcttttca acagttgctg aatccttgtg caaggcttta
     6361 aagtcgggta attcaataac gccagcttgt accatacgta actcatttac acggtctgtt
     6421 ctccataagc agtaatctac cgcttgctcc cagctaacac caaccacagg atattcttgg
     6481 taggctacat gcgaaaaata atactctaaa taaggttcat tataagctaa ctcgtccctc
     6541 cataccaatg tatcaggcaa tgctctgttt actaattttg gtgatgcaga aaacattaac
     6601 ctcatccaat acacatattc tctccaatct agattggaaa cttcgtgctg atccatataa
     6661 aaagtactaa ctgttacacg acgaggataa ttattccact cactgtatgt atcttcggta
     6721 acacgaccca tagtaaaggt acccccttct attaaaacca tgcctggag
//
LOCUS       DAOSMY010000034         6748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11375_length_6748_cov_3.636635, whole genome shotgun sequence.
ACCESSION   DAOSMY010000034 DAOSMY010000000
VERSION     DAOSMY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11375_length_6748_cov_3.636635"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..887)
                     /locus_tag="PLD76_01460"
     CDS             complement(63..887)
                     /locus_tag="PLD76_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOU26706.1"
                     /translation="MKILVLGANSYAGAAIYTQLREKYEVVGTYNAYPLFEELIQLDI
                     THAEEVEQIIKLHAPDTIVHVASNSNGNWCEQHPELAYAINVQATQRLVELANTYGAK
                     LVYISSLAATQGNNVYGKTKAVAEHYVQQTKAGFLILRPSLLVGFSPNTTNDRPFNRL
                     LKNITHHTPAVYDTSWQFQPTWLRHLCEVIDICIAQSLFNHIIPITVPQLCTRYSLAK
                     DILSDFGVTVLAEDKKDTTPLFVDTQDTLHALGLPVYSYQQMIDGIQEEISNFLRQ"
     gene            complement(988..1686)
                     /locus_tag="PLD76_01465"
     CDS             complement(988..1686)
                     /locus_tag="PLD76_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU26707.1"
                     /translation="MGNTLIRYKGVEICQKEQVVLKDVNLEIGEGEFYYLIGKVGTGK
                     SSLMKTMYAELPIETGEASLLDYDLLTITKSEIPFLRRQLGIIFQDFQLLIDRTVNEN
                     LLFVLKATGWKDKEEMQARIDAVLKEVGMSNKGYKMPHELSGGEQQRIVIARALLNKP
                     KLILADEPTGNLDPLTGAELVELLYTICKEENTTIIMATHNLGWIDAFPGKVLAISQN
                     KLCSGTKDECIFDY"
     gene            complement(1696..2289)
                     /gene="hisIE"
                     /locus_tag="PLD76_01470"
     CDS             complement(1696..2289)
                     /gene="hisIE"
                     /locus_tag="PLD76_01470"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOU26708.1"
                     /translation="MSLDFNKQGGLIPAIIQDSITNKVLMLGYMNEESLAKTKELGKV
                     TFYSRSKNRLWTKGEESGNFLHVVSIAEDCDNDTLLIKVNPVGPVCHTGADTCFFENN
                     VEDIMFLKFLQDFIDKRKQEMPEGSYTTSLFKKGINRMAQKVGEEAVETVIEATNGTD
                     EGFLYEASDLIYHLIVLLSSKGYRIEDLANELKKRHK"
     gene            complement(2306..3058)
                     /gene="hisF"
                     /locus_tag="PLD76_01475"
     CDS             complement(2306..3058)
                     /gene="hisF"
                     /locus_tag="PLD76_01475"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU26709.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFVNFRDAGDPVELGASYSKQGA
                     DELVYLDITASHEGRKTFVDMVKRVAANVSIPFTVGGGINELTDVEKLLNAGADKVSI
                     NSSAIRNPDLINQIAQHFGSQVCVVAIDANYEENDWICYLNGGRIRTDKRLFTWAKDA
                     QERGAGEILFTSMTHDGVKTGYANDALAQLSDMLKIPIIASGGAGSKNHFKDAFLQGK
                     ADAALAASVFHFGEINISELKDYLRKHDICVR"
     gene            complement(3074..3796)
                     /gene="hisA"
                     /locus_tag="PLD76_01480"
     CDS             complement(3074..3796)
                     /gene="hisA"
                     /locus_tag="PLD76_01480"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOU26710.1"
                     /translation="MIQLIPAIDIIDGKCVRLSQGDYAKKTVYNENPLEVAKMFQDSG
                     ITRLHLVDLDGAKAQHIVNHKVLYAIATHTNLVIDFGGGLKTDEDLNIAFENGASMIT
                     GGSIAVKNPTVFESWIQKFGNEKIILGADVHDKKIAVSGWVESTEHDLFPFLESYTTK
                     GINKVICTDIAKDGMLQGTSVDLYKEINMQFPELFLIASGGVSAMSDIYALDEAGIPA
                     VIFGKAIYEGKISLQEIARYNQ"
     gene            complement(3804..4397)
                     /gene="hisH"
                     /locus_tag="PLD76_01485"
     CDS             complement(3804..4397)
                     /gene="hisH"
                     /locus_tag="PLD76_01485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU26711.1"
                     /translation="MQKVVVIKYNAGNIYSVVHALRRLGVEPVVTADEAVIRASDKVI
                     FPGVGEATTTMRFLKERKMDELITSLTQPVLGICLGMQLMCKSSEEGNVDCLGIFDVP
                     VKLFVPTQQTQKIPHMGWNQLYDVKSNLFSEQLNEQFVYFVHSFYVPMCEHTIATTDY
                     IQPFSAALNKNNFYATQFHPEKSGDVGEQILKNFLAL"
     gene            complement(4399..5271)
                     /gene="purU"
                     /locus_tag="PLD76_01490"
     CDS             complement(4399..5271)
                     /gene="purU"
                     /locus_tag="PLD76_01490"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOU26712.1"
                     /translation="MPQSSTKDTAILLLHCPDKQGILAAVTEFININKGNIIYLDQHV
                     DFVEKEFFMRVEWEIESFLIPQEKISEYFYTLLGKKYGMNFRLYFNANKPRMAIFVSK
                     MSHCLFDLLSRYAANDWDVDIPFIISNHPDMEHVASKFDIPYYYFPITKETKQEQEAA
                     EIALLKEHKIEFVVLARYMQVLTAEFIAQYPQNIINIHHSFLPAFVGAKPYHAAYERG
                     VKIIGATSHYVTSELDAGPIIEQEVAKISHKDTVEMLIHKGQDLEKIVLSRGVEKHLE
                     RKVLVYKNKTIVFS"
     gene            5431..5898
                     /locus_tag="PLD76_01495"
     CDS             5431..5898
                     /locus_tag="PLD76_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOU26713.1"
                     /translation="MEVIILSSLLLAGIVLLLVELFLLPGSSIAGIGSALSFGGGIYY
                     AFAMYGVPVGIAVIVISVFVAAFAIYKFMKSKTLDTISLHTTIEGKNNPLEGISVSVG
                     DTGVAVSRLAPMGKVRINGNTIEVKVVSDMIDTGEQVKVTEISTSGIVVERNT"
     gene            5928..>6748
                     /gene="floA"
                     /locus_tag="PLD76_01500"
     CDS             5928..>6748
                     /gene="floA"
                     /locus_tag="PLD76_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839405.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOU26714.1"
                     /translation="MIESGMLTFIMVAVGFILLLIFFYYVPFLLWVSAKVSGVNVSLL
                     QLFLMRIRNVPPPILVRALIEGHKAGLEIKLDALEAHYLAGGHVERVVHALVSASKAN
                     IDLSFQMATAIDLAGRDVFEAVQMSVNPKVIDTPPVAAVAKDGIQLIAKARVTVRANI
                     KQLVGGAGEETILARVGEGIVSSIGSNESHKAVLENPDTISKLVLKKGLDAGTAFEIL
                     SIDIADIDIGKNIGAQLQMDQAQADKNIAQAKAEERRAMAVALEQEMIAKAQEARA"
BASE COUNT         2037 a         1364 c         1151 g         2196 t
ORIGIN      
        1 tgtcgaaatt gggctatatt gtttgtgcaa tcggtataac ttcgacatag cgagagaagg
       61 ttttattgtc ttaaaaaatt gcttatctcc tcttgtatcc catctatcat ctgttgataa
      121 ctatatacag gtaagcccaa tgcatgtagc gtatcttgtg tatctacaaa caaaggagtt
      181 gtatcctttt tatcttcggc aagcaccgta acaccaaaat cggacaagat atctttggct
      241 aaggagtagc gtgtacacag ttgtggcacg gtaataggaa tgatgtggtt aaagagtgat
      301 tgggcaatac aaatatctat tacctcgcac aaatgcctta accatgtggg ctgaaactgc
      361 cacgaagtat cgtaaacggc gggtgtatgg tgggtaatat tctttaaaag gcgattaaag
      421 ggtcggtcgt tggtagtatt cggactaaac cctaccagca acgacggacg aagaatgaga
      481 aaacctgcct tggtttgttg cacatagtgt tctgctactg ctttggtttt gccatacaca
      541 ttattgcctt gcgtagctgc tagcgaggat atgtacacaa gtttcgctcc ataagtattg
      601 gctagttcaa ccagcctttg tgttgcttgt acattaatgg cataggctag ttctggatgt
      661 tgctcgcacc aattgccatt ggagttagac gctacatgta caatagtatc aggtgcatgc
      721 aattttatta tttgctccac ctcttcggca tgcgtaatat ccagttgtat caattcttcg
      781 aataaaggat acgcattata cgtgcctact acctcatact tttctcttaa ttgggtataa
      841 atagctgctc ctgcataact attggctcct agtactaaaa tcttcatttc tatgtttatt
      901 acgcaatctt tttcttacac agatacaaca attacaaaca aaaaaaggga gaaaaactcc
      961 cttcattgta ttggtgtaaa acaaccatta atagtcaaaa atacattcat ctttggtgcc
     1021 actacacaat ttattttgag agatagccaa caccttacca gggaaagcat ctatccaacc
     1081 caaattatgt gtagccataa taatcgtagt gttctcttct ttacaaattg tatacagcaa
     1141 ttccacaagc tctgcacccg ttagcggatc gaggtttcca gtaggctcat ctgctaaaat
     1201 caatttgggt ttgttgagca atgcacgagc aattacaata cgttgctgtt cacctcctga
     1261 taattcgtga ggcattttat agcctttgtt actcatgcct acctctttta atacagcatc
     1321 aatacgagct tgcatttctt ctttatcttt ccagccagta gctttaagca caaacagtaa
     1381 attttcgttt accgtacggt caatcaacaa ctgaaaatct tggaaaataa tacctaactg
     1441 acgacgtaaa aatggaattt ctgattttgt tatcgttaat aaatcatagt ccaacaagct
     1501 tgcttcaccc gtttcaatag gcaattcagc atacatagtt ttcatcaaac tactttttcc
     1561 tgtacctact tttcctatca agtaatagaa ctccccttct cctatttcaa ggtttacatc
     1621 tttaagcaca acttgttctt tttgacaaat ttctacgcct ttgtagcgta tgagtgtatt
     1681 tcccatagca atttattatt tatgtctttt tttcaattcg ttggctaaat cttcaatacg
     1741 atatcctttt gatgacaata acacaattaa gtgatatatt aaatccgatg cttcgtacaa
     1801 aaatccttca tcagtaccat tggtagcctc aatcactgtt tcaacagcct cttcgcctac
     1861 tttctgagcc atacggttta ttcctttttt aaacaaagaa gtggtatatg aaccctctgg
     1921 catttcttgt ttgcgcttat caataaaatc ttgtaaaaat ttcaagaaca taatatcttc
     1981 tacattattc tcaaaaaaac aggtatctgc acccgtgtgg catacaggtc ctacaggatt
     2041 tacttttatc aatagggtat cattatcaca atcttcagca atagatacaa catgcaaaaa
     2101 attgccactt tcttctcctt ttgtccacag gcggttttta ctacggctat aaaaagtaac
     2161 ctttcccaat tcctttgttt tagctaatga ttcttcgttc atatagccca acatcaacac
     2221 tttattggta atactgtctt gtataatagc gggaattaag ccgccttgtt tgttaaaatc
     2281 taaactcatt gtattaatcg tttttttatc gaacacaaat atcgtgtttt cttaaataat
     2341 cttttagttc tgaaatgtta atttctccaa aatgaaacac acttgctgct aatgcggcat
     2401 ccgctttccc ttgtaagaat gcatctttga aatggttttt actacccgct cctcccgacg
     2461 caataatagg aatttttaac atatccgaca attgagccaa ggcatcattc gcatagcccg
     2521 ttttcacccc atcgtgtgtc atactcgtaa ataaaatttc gccagctcct cgttcttgtg
     2581 catcttttgc ccatgtaaat aagcgtttat ctgtgcgtat tcgtccacca tttagatagc
     2641 aaatccagtc attttcctca taatttgcat caatcgctac gacacatact tgcgagccaa
     2701 aatgttgagc aatttgattg attaaatccg gattgcgaat agccgatgaa tttatagaaa
     2761 ccttgtcggc tccagcattc aagagttttt ctacgtcagt taattcattg ataccaccac
     2821 ctactgtaaa cggaatactc acattagctg ccaccctttt aaccatatcc acaaaggttt
     2881 ttcttccctc atgcgaagcg gtaatatcca aatacactag ctcatcagct ccttgtttac
     2941 tataagatgc acctaattct acaggatcgc ctgcatctct aaaattcaca aaattaatac
     3001 cttttaccgt ttttccatct tttacatcca aacaaggtat aatacgtttt gctaacatct
     3061 caatttttgt tctttattga ttatatcgtg caatttcttg caacgaaatt tttccttcgt
     3121 aaatggcttt cccaaaaata actgctggaa tgcctgcctc atctaaggca taaatatctg
     3181 acatagcact tactccgcca ctagcaatta aaaataactc cgggaattgc atgttaatct
     3241 ctttgtataa atcaacagac gtaccttgta acataccatc tttggcaata tcggtacaaa
     3301 ttactttatt tattcctttc gtggtatatg actccaaaaa aggaaacaaa tcgtgttccg
     3361 tgctttctac ccaaccacta accgctatct ttttgtcgtg cacatctgca cccaatataa
     3421 ttttttcgtt accaaatttt tgaatccacg actcaaacac ggttggattt ttaaccgcaa
     3481 tacttccacc agttatcatc gaagcaccat tttcaaacgc aatgtttaaa tcctcgtctg
     3541 tttttagtcc accaccaaaa tctattacca aattggtatg tgtggcaatg gcataaagca
     3601 ccttatgatt aacaatatgt tgagctttag ctccatctaa atctaccaag tgcaaacgag
     3661 taataccact atcttgaaac atttttgcta cttcgagtgg attttcgttg tacaccgttt
     3721 tcttggcata atcaccttgt gataaacgaa cacatttacc atcgataata tctattgctg
     3781 gaatcaattg aatcatatat atattacaag gctaaaaagt ttttcaatat ttgctcacct
     3841 acatcgccac ttttttcggg gtgaaattga gtagcataaa aattattttt attcaatgcc
     3901 gcactaaaag gttgaatata atcggttgtt gcaatagtat gttcgcacat tggcacataa
     3961 aaactgtgca caaaatacac aaactgttca ttcagttgct ccgaaaacaa atttgatttt
     4021 acgtcataca actggttcca tcccatgtgt ggaatttttt gtgtttgttg tgtcggaaca
     4081 aataatttga caggcacatc aaaaataccc aaacaatcga cattaccctc ctccgaactt
     4141 ttacacatca actgcatgcc caagcaaatg cccaaaacag gttgcgtaag cgatgtaatc
     4201 agttcgtcca tcttgcgttc tttcaaaaaa cgcatcgtgg tagtagcttc tcccacacca
     4261 ggaaaaataa ccttatcaga agctcgtatt acagcctcat cagcagttac aacaggctct
     4321 acgcctaagc gtcgtaaagc atgcaccacc gaatagatat ttcctgcatt gtattttata
     4381 acgactactt tttgcatact aactaaatac gattgtttta ttcttataca caagcacttt
     4441 gcgttccaaa tgtttttcaa ccccacgcga aagtacaatt ttttccaaat cttgtccttt
     4501 atgaatgagc atttctaccg tatctttgtg cgaaattttc gcaacttctt gttcaataat
     4561 cggaccagca tccaactctg aagttacata atggcttgta gcaccaataa ttttcactcc
     4621 acgttcgtat gctgcatgat aaggttttgc tcctacaaat gctggtaaga acgagtgatg
     4681 tatattgata atattttgtg gatattgggc aataaactca gccgtaagca cttgcatata
     4741 acgtgcgagc actacaaatt caattttatg ttcttttaat agagcaattt ctgccgcttc
     4801 ttgttcttgt tttgtttctt tcgttattgg gaaataataa tacggtatat caaattttga
     4861 cgccacatgc tccatatctg gatgattact tattatgaat ggtatatcta catcccaatc
     4921 gttagctgca tagcgtgata ataaatcgaa taagcaatgc gacattttgg aaacaaatat
     4981 cgccatgcgt ggcttattcg cattaaagta taaccgaaaa ttcatgccat attttttgcc
     5041 aagcaaggta taaaaatact ccgaaatttt ttcttgcgga atcaaaaagc tctcaatctc
     5101 ccactctaca cgcataaaaa actctttttc cacaaaatca acgtgttggt cgaggtatat
     5161 aatatttcct ttgtttatat tgataaactc cgtaactgcc gccaaaattc cttgtttgtc
     5221 gggacagtga agcaataaaa ttgcggtgtc tttggtagat gattgtggca taagcggtac
     5281 ataaattcta aattccccac aaaattagtg tatttttact gaattatggc agatttaaac
     5341 gagatattac aaatatgtaa aaaaaataca acaaaaaaag aaagtaatca taattttgag
     5401 tatatttaca aactaaaatt tagttagaat atggaagtta tcattttaag tagcttattg
     5461 ctcgcgggta ttgttctctt gttagttgaa ctatttttat taccaggctc atctatagca
     5521 ggcataggct ctgcgttaag ttttggcggt ggcatttatt acgcttttgc catgtatgga
     5581 gtacctgtgg gtattgctgt tatagtaatt agtgtgtttg ttgcagcatt tgccatttat
     5641 aaatttatga aatccaaaac attggatacc atctcccttc atactaccat tgagggaaaa
     5701 aacaacccac tagaaggtat ttctgtatca gtaggagata cgggagtggc tgtttcgcga
     5761 ctagcaccta tgggtaaagt gcgaattaac ggcaatacaa tagaagtaaa agtggtatcg
     5821 gacatgatag atacaggcga acaagtaaaa gtaactgaaa tttctacgtc gggcattgtt
     5881 gtagaacgta acacataaaa aactattaat ttattaaata aaaaactatg attgaaagtg
     5941 gtatgttaac ctttattatg gtagcagtag ggtttatact gttattaatc tttttttact
     6001 acgttccgtt tttgctatgg gtatcagcca aggtatcggg cgtaaatgta tcgttgcttc
     6061 agttattttt gatgcgtatt cgaaatgtgc cacctcccat attggttaga gcactcatcg
     6121 aaggtcacaa agctgggtta gagattaaat tagatgcgtt agaagctcac tatttagcag
     6181 gtggacatgt ggagcgtgtt gtgcatgcat tggtttcggc gtctaaggca aatatcgatt
     6241 tgtcatttca gatggcaacg gctattgatc ttgcaggtag agatgtattt gaagctgtac
     6301 agatgtcagt taatcccaaa gtgattgaca ccccaccagt agctgcagta gctaaagatg
     6361 gtattcagtt aattgctaaa gcccgcgtaa cggtacgtgc aaatatcaaa caattagtgg
     6421 gtggagctgg cgaagaaact attttagctc gtgtaggaga aggaattgta tcttctattg
     6481 gttctaacga atcgcataaa gcagtattag aaaatccaga tacgatttca aaactcgtat
     6541 taaaaaaggg attagatgcg ggtacggcgt ttgagatttt atccattgat atcgctgata
     6601 ttgacatagg taaaaacatt ggggctcaat tgcaaatgga ccaagcacaa gcagataaaa
     6661 acattgcaca agccaaagcc gaagagcgta gagctatggc ggttgccctt gagcaagaaa
     6721 tgatagccaa agcacaagaa gcacgtgc
//
LOCUS       DAOSMY010000035         6689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11507_length_6689_cov_3.216159, whole genome shotgun sequence.
ACCESSION   DAOSMY010000035 DAOSMY010000000
VERSION     DAOSMY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11507_length_6689_cov_3.216159"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="trxB"
                     /locus_tag="PLD76_01505"
     CDS             <1..891
                     /gene="trxB"
                     /locus_tag="PLD76_01505"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU26715.1"
                     /translation="TAAIYASRANLSPILYEGMQPGGQLTTTTEVENFPGYPEGITGV
                     EMMEDLKKQAIRFGTDVRWGSATEVDFSIRPFVVTIDGEKTITADSVIIATGASAKYL
                     GIPDEQKYAGSGVSACATCDGFFYRKKNVAVVGGGDTACEEAIYLSSICNKVYLIVRR
                     NELRASKVMQKRVIETANIEVLFEHQTKSLFGEKVVEGAILWHKKGTPEEKEVSINID
                     GFFLAIGHTPNTRIFKNFIEMDEVGYIKTKNGSTKTNIEGIFAAGDVADPVYRQAITS
                     AGSGCKAAIDAEKYLHENGL"
     gene            complement(941..2050)
                     /locus_tag="PLD76_01510"
     CDS             complement(941..2050)
                     /locus_tag="PLD76_01510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU26716.1"
                     /translation="MRKKQIILSVTNDLETDQRVHKVATFFYDKGFDVLVIGRKLKNS
                     QNLSRSYRTKRFNLFFNKSVLFYTEYTIRLFVFLLFSRKSVLWSNDTDTIIPNFIISK
                     LYKRVLFFDAHELFPELPELAHRPKIKMIWKKIEDCIFPHIQNSFTVCQSIADYYKEL
                     YGIEMQVIRNVPYKKDIRSYQPISVPKDKIIVLYQGAVNVGRGLEQSIEAMTLLPNAM
                     LYIVGDGDVLDQLKKQVQHLNVQQQVVFTGKIPFEDLPRYTKVAHVGLALLEHRGLSY
                     YYALPNRIFDFIQADVPILARNFPEMARIINGYGVGITIDANTPSLIAKTIQDIYNQQ
                     HQYTLSSFDKAKEDLCWEKEQCILFEALKKSGLLN"
     gene            2201..3217
                     /gene="murB"
                     /locus_tag="PLD76_01515"
     CDS             2201..3217
                     /gene="murB"
                     /locus_tag="PLD76_01515"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOU26717.1"
                     /translation="MRTLENFSLLNHNTFGVDVTANYFVEYDNITELQQLLGSDLLKE
                     NKFFHIGGGSNLLFINDYEGVILHSQIKGKEISKESADYVWIRVGAGENWDDFVDYCV
                     ENQLGGAENLSLIPGEVGATPIQNIGAYGVEVKDIIQSVDCIEISTGKQKTFSNKECA
                     FGYRDSIFKQGFRNKYVVCYVTYKLAKHYTPNVSYGSLSTEILNYKEVNIQTVRQAVI
                     AIRKRKLPEPKVLGNAGSFFMNPIIAMEHFLQLQQLYPDIPSFHVSDKHIKVPAGWLI
                     EQCGWKGKSIGKVAVHGSQALVLINKGGANGLDIIYLSDKIRESVKEKFNIDIIPEVN
                     IIFG"
     gene            complement(3204..4394)
                     /locus_tag="PLD76_01520"
     CDS             complement(3204..4394)
                     /locus_tag="PLD76_01520"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU26718.1"
                     /translation="MLPQEIKHLIQRYKDKPLESVVFKKFPENVDVPLLLQQLEGRRF
                     IANKMPSWNTFIDELVFPSHLAFQQASSEQTARYKASLLQIESLVDLTGGMGIDSYFF
                     SQRVKKLVYIEQQSNLCSIASHNFNVLGAQNIDVINTQAEVYSNTMPMVDCVYIDPAR
                     RNITGAKVVLLEDCSPNVEAILPHILTKTRYVLLKLSPMFDIEMAVRSLPNIKEIHIV
                     AVDNDCKELLVVLDVLHSLPSCTYHCINIAENNQNQQHSYTNELVQGVLIHYTSQLST
                     YVYEPNVTILKAGCFSSIATAFCVEKLHPNSHLFTSNQLHIDFPGRIFEVIEVVEFNK
                     KAIKTLLTPIKKANVTVRNFPLRVDEFRKKTQLKEGGDTYIFATTVANNKKVCIICRK
                     INQK"
     gene            complement(4394..5860)
                     /locus_tag="PLD76_01525"
     CDS             complement(4394..5860)
                     /locus_tag="PLD76_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOU26719.1"
                     /translation="MKKILNIKIIIRTLGFLLLFQSFFMLLASFVSFLYNESDVQPIL
                     LSAGITLTVALIAILSISKPTEPIGKREAYLVVALVWVVFSLFGLLPFLLSGSIPSFT
                     DAFFETMSGFTTTGASILTNVESLSHGLLFWRSIIQWLGGMGIIVLSLAILPLLGMGG
                     MQLFVAEVPGPTKDKLHAKVSFTAKALWGIYVVLTASQVLFLWLGGMDLFDGICHSFT
                     TMATGGFSTKNASIAYWNSPLIEYIIIVYMFLAGVNFSLYFSFLQGNFSKIFKNEELK
                     YYLSFVLVFTLLVVCSVVFYTPELVISVEQIIRHSLFQVVSLFTTTGFATYDYLLWSQ
                     FTWMLLIVTMLFGASAGSTSGGIKLVRIVLLLKNSYYEFKRLLHPNAIIPVRYNEKVV
                     MPQMMTNVLAFVALYITILVIGMIMLGAFGMNFDEAVGSAVTALSNVGPSLGSLGPSS
                     NFAAISLPAKWVMSFLMLIGRLELFTVLVILTPTFWSK"
     gene            complement(5869..>6689)
                     /locus_tag="PLD76_01530"
     CDS             complement(5869..>6689)
                     /locus_tag="PLD76_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOU26720.1"
                     /translation="KENSNVRIIMIKRLGMDIIAKGKDKILAGDIVYFITTKDNIEQV
                     RIQAGKEDFEINNIMILGGGSRIGVRAIQELPRNKNIKVIEQNKEKSIRVIENTDNVL
                     VINGDGRDLELLKNEDIESYDAFVALTGNAETNILACLTAKNFGVKRTVAEVENLDYV
                     PLAENLNIGSIINKKSLAASHIYKLILGGNVSNVRCLTLADVEIVEFVAGEKSAIVKT
                     PIKDIRLPENVNIGGIIRDGVGFVAMGTSHIQPGDNVLVFCAEDTIRKVEKLFK"
BASE COUNT         2349 a         1174 c         1081 g         2085 t
ORIGIN      
        1 actgctgcca tttatgcttc aagagcaaat ttatccccta ttttatatga gggaatgcaa
       61 cctggtggac aattgacaac aaccaccgaa gtagaaaatt ttccaggtta tccagaagga
      121 attactggag tagaaatgat ggaagacctt aaaaaacaag ccatacgatt tggaacagat
      181 gtacggtggg gctctgctac cgaagttgat ttttcaatac gtccatttgt agttaccatt
      241 gatggagaaa aaacaattac agcagattct gtcattatcg caacaggggc atcagcaaaa
      301 tatttgggca tccctgatga acaaaaatat gctggttctg gtgtatctgc atgtgctaca
      361 tgcgatggat tcttttatag aaaaaaaaat gtagcagtag ttggtggagg agatactgcc
      421 tgcgaagaag ctatatacct atcatctatc tgtaataaag tatatttgat tgttcgtaga
      481 aatgaactaa gagcatcaaa ggtaatgcaa aaacgcgtta tcgaaacggc aaacatcgaa
      541 gtgttattcg aacatcaaac aaaatcactt tttggtgaga aagtggtaga aggggctata
      601 ttatggcaca aaaaaggaac tccagaagaa aaagaagtga gcattaacat tgatggattc
      661 tttttagcca taggtcacac tccaaataca agaatcttca aaaactttat agaaatggac
      721 gaagtaggct atattaaaac taaaaatggt agtactaaaa ccaatattga agggatattt
      781 gccgctgggg atgtagccga cccagtatac agacaagcta tcacttctgc tggtagtggg
      841 tgtaaagcag ccatagatgc tgaaaaatat ctacacgaaa acggattata atagctaaat
      901 atgtatcata caaccttatc aaaaatggtg aggttgtaga ttaattcaat aaaccgctct
      961 ttttaagtgc ttcgaataat atacattgct ctttttccca acataaatcc tcttttgcct
     1021 tgtcaaatga actcaaagtg tattggtgct gttggttata tatatcttgt atagttttag
     1081 caataagtga tggagtgtta gcatctatgg ttattccaac accatatcca ttaataattc
     1141 gtgccatttc tggaaaatta cgtgctaaaa taggtacatc agcttgaata aaatcaaata
     1201 ttcgatttgg aagggcataa taataactca aacctctatg ctccagtaag gctaacccaa
     1261 catgtgctac tttagtgtag cgaggtaaat cttcaaaagg aattttgcct gtaaaaacaa
     1321 cttgttgttg tacattcaaa tgttgaactt gtttttttaa ctgatctaac acatcgccat
     1381 ctcctactat atacagcata gcattcggca ataaagtcat tgcctcgata gattgctcta
     1441 aacccctacc tacatttacc gctccctgat aaagaacaat tatcttatcc tttggtaccg
     1501 atatgggttg gtaagaacgt atatcttttt tataaggaac atttctaatc acttgcattt
     1561 caataccata caattcttta taataatctg ctatggattg gcaaaccgta aaactatttt
     1621 gtatatgagg aaaaatacaa tcttcaattt tcttccaaat cattttaatt ttcggtcgat
     1681 gtgccaactc aggcaactcg ggaaataatt cgtgagcatc aaaaaacaag acccttttgt
     1741 acaacttaga gataataaaa ttagggataa tagtatcggt atcattcgac cataaaacag
     1801 attttcgaga aaaaagtaaa aaaacaaata atcgaatagt atattcagtg taaaataaaa
     1861 cactcttatt gaaaaataga ttgaatcttt ttgtacgata tgatcgggat aaattttggc
     1921 tatttttaag tttacgacca attactaaaa catcaaaacc cttatcataa aaaaaagtag
     1981 ctaccttatg tacacgttgg tctgtttcca aatcgttggt aactgaaagt atgatttgct
     2041 tttttctcat gttacaaaag tacgtttttt ttactatttg tagctcttag aagtataaat
     2101 tttatatctt tgtacaacaa acaaagatgg acaattatta taaaaaaccg aaaattgatt
     2161 tatacaattt tagtaatact aatacaatat ccactattat atgagaacac tggaaaattt
     2221 ttcactactt aatcataata catttggagt cgatgttacg gcaaattatt ttgtggagta
     2281 tgataacatt actgaactgc aacaattatt aggaagtgat ttgttaaaag agaacaaatt
     2341 ttttcatatt ggaggtggca gtaatctgct ctttatcaac gactatgaag gggttatttt
     2401 acattcgcaa ataaagggaa aagagataag caaggaatca gctgactacg tatggatacg
     2461 tgtaggtgca ggagaaaatt gggatgattt tgtagattat tgcgtagaaa atcaattagg
     2521 aggtgctgaa aatttatctt taattccagg cgaggtaggt gctactccaa ttcaaaatat
     2581 tggggcttat ggtgttgaag taaaagatat tattcaatca gtagactgta tcgaaattag
     2641 cactgggaaa caaaaaacct tttcaaataa agagtgtgct tttggataca gggatagtat
     2701 attcaaacag ggtttccgta ataaatatgt tgtttgctat gttacgtaca aattagctaa
     2761 acattatacc cccaatgtaa gttatggaag tttatctact gaaatcttga attataaaga
     2821 agtgaacata caaacggtta gacaagccgt aattgccatc cgaaaacgaa aactccctga
     2881 acctaaggta cttggtaatg ctggtagttt ttttatgaat cccatcatag caatggaaca
     2941 ttttcttcaa ttacaacagt tgtatccaga tatcccaagt tttcatgtat ctgataagca
     3001 tattaaagtt cctgcaggtt ggttaattga acaatgtgga tggaaaggaa aatcaattgg
     3061 caaagtagca gtacacggta gccaagcatt ggtactaatc aacaaaggtg gagcaaatgg
     3121 attagacatt atttatcttt cggataaaat aagagaatct gtaaaagaga aatttaatat
     3181 tgacattata cctgaagtaa acattatttt tggttaattt ttcgacagat aatacatact
     3241 tttttattat ttgctaccgt tgtagcaaat atatatgtat caccaccctc ttttaattgt
     3301 gtttttttac ggaattcatc cactcgtaaa ggaaaatttc gcaccgttac gtttgctttt
     3361 ttaatgggtg ttagtagtgt tttgatagcc tttttattaa attctaccac ttctattacc
     3421 tcaaatattc tacccggaaa atcaatatgc aattgatttg aagtaaataa atgactattg
     3481 ggatgaagct tttctacaca aaatgcagtt gcaatactac taaaacaacc tgcttttaaa
     3541 atggtaacat ttggttcgta tacataggtc gaaagttgag atgtataatg aatgagtacg
     3601 ccttgtacaa gctcatttgt ataactatgt tgttgatttt ggttgttttc agcaatattg
     3661 atacagtgat acgtacaact aggtaacgaa tgaagaacat caagcacaac aagcaattct
     3721 ttacaatcat tatcaactgc tactatgtgt atttccttta tatttggcaa acttctcacc
     3781 gccatttcta tatcaaacat aggtgataat ttgagtaata cataccttgt ttttgtaagt
     3841 atgtgaggta atatagcctc cacattaggg ctacaatctt ctaacaatac gactttagct
     3901 cccgtaatat tcctgcgtgc agggtcgata tacacacaat cgaccattgg catagtgtta
     3961 ctatatactt cagcttgtgt atttataaca tcaatattct gtgcacccaa tacgttaaaa
     4021 ttatgagatg ctatgctaca taagttactt tgttgttcaa tatacactaa ttttttcact
     4081 cgttgcgaaa agaaataact atctatgccc atcccacctg ttaaatcaac aagagattct
     4141 atttgtagca aagaggcttt gtagcgtgct gtttgctcag aagatgcttg ttgaaaagcc
     4201 aaatgcgaag gaaatacgag ctcatcgatg aatgtattcc aagaaggcat tttattagca
     4261 ataaatctac gaccttctaa ttgttgcagt aacaatggga catctacatt ctctggaaat
     4321 tttttaaaaa caacagattc tagaggttta tctttgtaac gttgaataag atgcttaatt
     4381 tcttgtggta gcattattta ctccaaaatg tgggtgtaag aataaccaaa actgtaaata
     4441 attctaaacg accaattaac attaaaaaag acataaccca tttggcaggg agagaaatag
     4501 cggcaaaatt agatgatggt ccgagactac ctaaactggg tcctacgttg cttaatgcag
     4561 taacagccga acctaccgcc tcatcaaaat tcattccaaa agctccaagc ataatcattc
     4621 caattactaa tatggtaata tagagagcaa caaaagccaa cacgtttgtc atcatttgag
     4681 gcataactac tttttcatta taacgtactg gaataattgc atttgggtgc aacaaacgtt
     4741 taaactcata ataactattt ttgagtaata acacaatgcg aaccaacttt attccaccac
     4801 tagttgatcc agctgatgcc ccaaataaca tcgtaacaat caacaacatc catgtaaatt
     4861 gagaccaaag taaataatca taggtagcaa aacctgttgt tgtgaacaaa gaaaccactt
     4921 gaaataaact atgtcgaata atctgttcaa cacttattac taactcagga gtataaaata
     4981 cgacgctaca aactactaat agagtaaata caagaacaaa tgaaagatag tactttaatt
     5041 cttcattttt aaaaatcttt gaaaaatttc cctgtaaaaa agaaaaatag agcgaaaaat
     5101 taactcctgc caaaaacata tacacaatga ttatatactc aatcaaagga gaattccaat
     5161 aagcaatact ggcattttta gtagaaaaac ctcccgttgc cattgtcgta aatgaatgac
     5221 aaataccatc aaataaatcc atccccccaa gccataaaaa caagacttgc gatgctgtta
     5281 aaactacata aataccccac aatgctttag ctgtaaaact aactttagca tgcaatttat
     5341 ctttggtagg tcctggtact tctgcaacaa aaagctgcat tccacccatg cccaatagag
     5401 gcaaaatagc caatgaaagc actataatac ccatacctcc cagccattgg ataatactcc
     5461 gccaaaacaa taatccatga gaaagacttt ctacattggt taagatagat gctcccgtag
     5521 tggtaaaacc agacattgtt tcaaaaaaag catccgtgaa agaaggaatg gaaccactca
     5581 ataaaaaagg aagcagccca aacaatgaaa aaacaaccca aactaatgct actactaaat
     5641 aagcttctcg ttttccaata ggttctgttg gtttggaaat agataagata gcaataaggg
     5701 caacggtcaa agtaatgcct gcagacaata aaatgggctg tacatctgat tcgttataca
     5761 aaaaagaaac gaaagatgcc aacaacataa aaaagctttg aaacaataac aaaaagccta
     5821 atgttcgaat aattattttg atatttaaaa tttttttcat taattgagct acttaaataa
     5881 cttctctact tttctaattg tatcttcagc acaaaaaacg agcacattat caccaggttg
     5941 aatatgagaa gttcccatgg caacaaagcc tactccatct ctaattatac ctccgatatt
     6001 tacattctca ggcaaacgaa tatctttaat tggggtttta actatagccg atttttctcc
     6061 tgctacaaac tctacaattt ctacatcagc aagggttaag caacgtacat tagacacgtt
     6121 gccaccaaga attagtttat atatatgact ggcagctaat gattttttat ttataattga
     6181 ccctatattt agattttctg ccaaaggtac ataatctaag ttttcgactt cagcaaccgt
     6241 acgttttacc ccaaaatttt tggctgttaa acatgctaat atatttgttt ctgcattacc
     6301 cgtgagggcg acaaaagcat cataagactc aatgtcttcg tttttcaaca attctaaatc
     6361 acgtccatct ccgttaataa ccaatacatt atcggtattt tcaatgacac gtatactttt
     6421 ttctttattt tgttcaataa cctttatatt tttattcctt ggtaattctt gtatcgcacg
     6481 tacaccaata cgacttcctc ctcccaaaat cataatatta ttaatctcaa agtcttcttt
     6541 tcctgcctga atacgtactt gttcgatatt gtcttttgtt gtgataaaat atacaatatc
     6601 tccagccaat attttatctt ttcctttggc aataatatcc attcctaatc gcttaatcat
     6661 aatgatacgc acgttagaat tctctttat
//
LOCUS       DAOSMY010000036         6671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11555_length_6671_cov_3.567110, whole genome shotgun sequence.
ACCESSION   DAOSMY010000036 DAOSMY010000000
VERSION     DAOSMY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11555_length_6671_cov_3.567110"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="PLD76_01535"
     CDS             complement(<1..90)
                     /locus_tag="PLD76_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU26721.1"
                     /translation="MEVIFLGTGTSTGVPYINCPCDVCQSTDSK"
     gene            complement(97..1461)
                     /locus_tag="PLD76_01540"
     CDS             complement(97..1461)
                     /locus_tag="PLD76_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOU26722.1"
                     /translation="MQLPVDFIHGMKGLLGESDYSLFFEALQQEPPVSIRLNTKFTSA
                     QVKEYQSVSWCKNGYYLPSRPLFTADPLFHAGAYYVQEASSMFIEQIIKQHVQHDVIA
                     LDLCAAPGGKSTHLASLLSTNSLLVSNEFIRSRANILAENCMKWGNGNMIITSNSSDE
                     IAQLESFFDFILVDAPCSGEGMFRKDVNAINEWSLLNVEMCVTRQRHILKQAWQALAE
                     DGFLVYSTCTFNEKENEENIKWLIDELGAEYIPIGMDNFPEITNTDVGYRFYPHKTKG
                     EGLFMALVQKKSACNKQNIKLKTDKNKQNIADIEALNLYINDSSDWIIQQNNQQYRAI
                     LASKQQEIKWIEKHLYVLFSGIQLAEKKGNDFVPHEHLALSKQLNTNSFQVVDVDWIT
                     AIQFLKREALFLPDASKGYVLLQYNNVPLGWVKNIGNRCNNMYPSSWKVRMDISHFTN
                     IQII"
     gene            1525..2229
                     /locus_tag="PLD76_01545"
     CDS             1525..2229
                     /locus_tag="PLD76_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU26723.1"
                     /translation="MSAKLYLLPTTLGESEINAVLPSTNNEICSQLKHYIVEDVRTAR
                     RFLKKVDKTIDIDSIQFYVLNKHTTAEELSTFLKPLVAGFDMGVLSEAGCPAIADPGA
                     DVVRMAQEKNIQVVPLVGPSSIILSLMASGFNGQNFAFNGYLPIQDDERIAMLKKLEK
                     KVYNEQQTQIFIETPYRNMKLVADIVQHCMPNTKLCIATDITLESEYIKTKTNAEWKK
                     GIPDLHKRPCIFLLYK"
     gene            complement(2230..3000)
                     /gene="kdsB"
                     /locus_tag="PLD76_01550"
     CDS             complement(2230..3000)
                     /gene="kdsB"
                     /locus_tag="PLD76_01550"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOU26724.1"
                     /translation="MNTLRFLAIIPARYASTRFPGKPLAILDGKTMIQRVYERVSSEI
                     QDVYVATDDERIALEVARFGGKYVMTSPLHTSGTDRCYEAFTKIRCDFDVVINVQGDE
                     PFIHPEQISLLKTCFDDSKTEIATLVKPYSYDDIVDNLATPNTPKVVITKDNYALYFS
                     RSIIPYKRGTDRHIWLQTHTYYKHIGMYAYKHTVLKEITQLEPSSLEKAESLEQLRWL
                     ENGYRIKVAITEHERIGIDTPDDLAQALTFLKNQKRID"
     gene            complement(3024..4160)
                     /gene="hisB"
                     /locus_tag="PLD76_01555"
     CDS             complement(3024..4160)
                     /gene="hisB"
                     /locus_tag="PLD76_01555"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOU26725.1"
                     /translation="MDNLKKALFIDRDGTIIAEPPVTFQVDNLQQLEFLPSVIRNLYL
                     IRKHTDFEFVMVTNQDGLGTEVYPQENFDSVQAKMLQVLQNEGVVFDDILIDTSFPEQ
                     NLPTRKPQTGLLTSYIAGGYDLQHSFVIGDRITDVELAKNIGCKAIFLQNIENATSIL
                     EKHKLADYCALISTDWDEIAAYLVAKERTATVQRTTKETDILVYVNLDGKGKTSIHTG
                     LGFFDHMLDQIGKHSGMDLTIHVKGDLQVDEHHTIEDTALALGEALLVALGNKRGVER
                     YGYCLPMDECLCQVALDFGGRPWLVWDATFTREKVGDMPTEMFMHFFKSLSDTAKMNL
                     NIKAEGANEHHKIEGIFKALARAIKMAIKRDVFNGELPSTKGVL"
     gene            complement(4172..5206)
                     /gene="hisC"
                     /locus_tag="PLD76_01560"
     CDS             complement(4172..5206)
                     /gene="hisC"
                     /locus_tag="PLD76_01560"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU26726.1"
                     /translation="MNVENLVRKNILALKPYSCARDEFQGEASVYLDANENPMNAPYN
                     RYPDPLQWKLKEKIAKLKSVNPSQIMLGNGSDEPIDLVIRIFCEPLVDNIVAIDPTYG
                     MYKVCADVNNVEYRKVLLNDDYSLDATRVMNAVDKHTKIIFLCSPNNPTGNLLNRNEM
                     KHIIQNFDGIVVIDEAYVDFSPEQSWLVEINTYQNIIVLQTFSKAWGLASVRCGMAFA
                     SEKIISFFNKVKYPYNINILTQQFVEEQLENTDRKEEWVTMLLGQRTYLVEQLNKLPL
                     IQTIYQTDANFVLVKVEDANAIYRYLVDLGIIVRNRNTVSLCLGCLRITIVTQQENDT
                     LLQALKAYTY"
     gene            complement(5370..5442)
                     /locus_tag="PLD76_01565"
     tRNA            complement(5370..5442)
                     /locus_tag="PLD76_01565"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5407..5409),aa:Lys,seq:ttt)
     gene            complement(5664..6167)
                     /locus_tag="PLD76_01570"
     CDS             complement(5664..6167)
                     /locus_tag="PLD76_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26727.1"
                     /translation="MKKTVVACLMLLGGITMIVAQTDNRAEMRAAKMQEMQAQKIAFI
                     KEKVSFTDKESAEFFPLYQEFEQQRMELNRKSRPQGKGFKAKNTELSEQEKEAVMDNF
                     IQNRVETANLEKAYYEKFKKILPASKLFEVYQADREFKQELLQDFRDRGAQNQDKKSR
                     ENKSPKK"
     gene            complement(6258..6602)
                     /locus_tag="PLD76_01575"
     CDS             complement(6258..6602)
                     /locus_tag="PLD76_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26728.1"
                     /translation="MEINDNSRNEVGNKVPFTTPDNYFEEFSAKIEQLLDKQEESNQV
                     INLSLWQRVQPYVYLAAMFIGLYVSITTFVKPSIQQKQKEQELVELAIQKELLLDEID
                     DYALYELLSYNN"
BASE COUNT         2063 a         1238 c         1028 g         2342 t
ORIGIN      
        1 tttactatca gtagattgac atacatcgca aggacaatta atatatggaa caccagtaga
       61 tgtgcctgtt cctaaaaata tgacttccat tatggtttat atgatttgaa tgtttgtaaa
      121 atgtgaaata tccatacgta ctttccacga acttggatac atattattac acctgttgcc
      181 tatatttttg acccatccta atggtacatt attgtattgc aacaacacat aaccttttga
      241 tgcgtcgggt aaaaacaagg cttctcgttt aagaaattga atggctgtta tccaatcaac
      301 atctactact tgaaaagaat tagtattaag ttgtttagaa agggctaagt gctcgtgagg
      361 aacaaagtca ttgccttttt tttcagccag ttgaattcca gaaaacaata catataagtg
      421 tttttcaatc catttaattt cttgttgttt ggaggctaat attgctctgt actgttggtt
      481 attttgctgt attatccaat ctgacgaatc gtttatgtat aaattaagag cctcaatatc
      541 agcaatattt tgtttattct tgtctgtttt tagttttata ttttgtttat tacaagcact
      601 ctttttttga actaatgcca taaacaagcc ttctcctttt gttttgtgtg gataaaaacg
      661 ataaccaaca tctgtatttg ttatttcagg gaagttatcc atgccaatcg gtatatattc
      721 agcacccaat tcgtctatca accattttat attttcttcg ttttcttttt cattaaaggt
      781 acaggtgctg taaaccaaaa aaccatcttc tgctaatgct tgccacgctt gttttaaaat
      841 atgccgttgc cgagttacac acatttctac atttagcaac gaccactcat tgatggcatt
      901 tacatctttg cgaaacatac cttcgccaga gcaaggggca tctaccaaaa taaaatcaaa
      961 aaaactttct aattgagcaa tttcatcaga agaattggag gtaataatca tatttccatt
     1021 tccccacttc atacaatttt ctgctaaaat attggctctt gaacggataa actcgttgct
     1081 taccaacaaa ctatttgttg agagcaaaga agctaaatgt gtacttttcc ctcctggagc
     1141 agcacacaaa tccaaggcta ttacatcgtg ttgtacgtgt tgtttaataa tttgttctat
     1201 gaacatagaa cttgcctctt gtacataata agctcctgca tgaaacaatg gatcagctgt
     1261 aaataaaggt cgtgaaggta aataatatcc atttttacac caacttactg attggtattc
     1321 ttttacttgt gcagatgtaa atttggtatt taatcgaatt gaaacgggag gttcctgttg
     1381 taaagcctca aaaaataatg aatagtcaga ctcgcctagc aagcctttca taccatgaat
     1441 aaaatctaca ggaagttgca tagttcatta ttaagtacac aaaagtacaa tttttttagt
     1501 aattttgcag aaaataccaa ctatatgtct gcaaaattat acctattacc aacaaccctt
     1561 ggcgaatcag aaatcaatgc tgtactaccc tctacaaaca atgaaatttg ctcgcaacta
     1621 aaacactata tagtagagga tgttcgaaca gcccgacgat ttttaaagaa agtggataaa
     1681 accattgata ttgatagcat tcaattttac gtattgaaca aacacactac tgctgaagag
     1741 cttagcacat ttttgaagcc cttagtagca ggattcgata tgggggtatt atcagaggct
     1801 ggttgtcctg caattgccga cccaggtgca gatgtggtac gtatggctca agaaaaaaat
     1861 atacaagtag ttccattagt tggaccttct tctattattt tatcgctcat ggcatcagga
     1921 tttaatggac agaattttgc atttaatggc tatcttccca ttcaagatga tgagcgtatt
     1981 gcaatgctta aaaaattaga gaaaaaagtg tacaacgagc aacaaacaca gatttttatc
     2041 gaaactcctt atcgcaatat gaaattagtt gcagacattg tacaacattg tatgccaaac
     2101 acgaaattat gcattgctac ggacatcaca ttagaaagcg aatatatcaa aacaaaaaca
     2161 aacgctgaat ggaaaaaagg tattcctgat ttacacaagc gtccttgtat atttctgctt
     2221 tacaagtaat tagtctattc ttttttgatt tttcaaaaat gtaagagctt gtgccagatc
     2281 atcaggtgta tcaattccta ttctttcgtg ttcagttatt gccactttaa tacgataccc
     2341 gttctccaac caacgcagtt gctctagcga ctctgctttt tcgagtgatg atggctctaa
     2401 ttgcgtaatt tcctttaaaa cagtatgttt ataagcgtac atgcctatgt gtttataata
     2461 ggtgtgagtt tgtaaccaaa tatgtctatc tgttcctctt ttatacggaa taatcgaacg
     2521 actaaaatac aacgcgtaat tatctttcgt aataaccact tttggagtat taggtgttgc
     2581 taaattatct acaatatcat cgtacgaata cggtttaact aaggtggcaa tttcggtttt
     2641 ggagtcatca aaacaagttt taagtaaact tatttgttca ggatgaataa aaggctcatc
     2701 tccttgcaca ttaataacaa catcaaaatc acacctaatt tttgtaaaag cctcgtaaca
     2761 cctatctgta ccgcttgtgt gtaacggact tgtcattaca tatttacctc caaaacgtgc
     2821 cacctccaat gcaatacgct catcatcagt agccacatac acatcttgta tttcggaaga
     2881 aactcgttcg tatacgcgtt gaatcatggt ttttccatca agtatagcca gaggcttgcc
     2941 aggaaaacgt gttgatgcat aacgtgctgg gatgatagcg aggaaacgta atgtattcat
     3001 ttttgaagta ttataaaaaa taattataaa acacctttcg tactaggcaa ttctccatta
     3061 aaaacatccc ttttaatagc cattttaata gcacgagcaa gggctttaaa tattccttca
     3121 attttatgat gttcgttggc tccttcagct tttatattca aattcatttt agcagtatcg
     3181 ctcaatgatt taaaaaagtg cataaacatt tctgttggca tatctcctac tttctctctt
     3241 gtaaaagtag catcccatac caaccagggt cgtccaccaa aatccaaagc aacttgacat
     3301 aagcattcgt ccataggcaa acaatagcca taacgctcta cacctcgttt gtttcctaaa
     3361 gctactaaaa gggcttctcc caaagctaaa gcagtatctt ctatggtatg atgctcatca
     3421 acttgcaaat ctccttttac gtgtatggta agatccattc ccgaatgttt accaatttgg
     3481 tctaacatat gatcgaaaaa acctaaacca gtatgaatag aagttttacc tttgccatct
     3541 aaattaacat acactaaaat atctgtttct ttggtggttc gttgcacagt agcggtgcgt
     3601 tcttttgcaa ctaaatatgc agcaatttcg tcccaatccg ttgatattaa ggcacagtaa
     3661 tctgctagtt tatgtttctc caatatgctt gttgcatttt ctatgttttg caaaaaaatt
     3721 gctttacaac cgatattttt tgccaattct acatctgtaa ttctatctcc aattacaaaa
     3781 gaatgttgta aatcgtaacc tccagcaatg taggatgtta acaatccagt ttggggtttt
     3841 cgtgtaggca aattttgctc aggaaaagaa gtatcaatca aaatatcgtc gaatacaact
     3901 ccctcatttt gtaatacttg caacattttt gcttgtaccg aatcaaaatt ttcttgagga
     3961 taaacctctg ttccaagccc atcctgattg gttaccataa caaactcaaa atcagtatgt
     4021 ttgcgtataa gatacaaatt acgaattact gatggcaaaa actccaattg ttgtagatta
     4081 tctacttgaa atgtaacagg aggctctgca atgatagtac catctctatc gataaaaagg
     4141 gcttttttca gattatccat tcgttataga attagtatgt atatgctttt aaagcttgta
     4201 atagtgtgtc attttcttgt tgtgttacaa ttgttattcg caaacatcct aaacacaatg
     4261 aaaccgtgtt tctatttcga acaatgatac ctaaatcaac taaataacga taaatggcat
     4321 tagcatcttc tacttttacc aacacaaaat tggcatcggt ttggtatatt gtttgtatga
     4381 gaggaagttt gtttaattgt tccactagat aagtacgttg tcctaacagc atagttaccc
     4441 attcttcttt tcggtctgta ttttcaagtt gctcttcaac aaattgttga gttaatatat
     4501 tgatattgta gggatactta actttattga aaaaagagat aattttttca gaagcaaaag
     4561 ccattccaca ccgcacagaa gccaagcccc acgcttttga gaatgtttgt aacacaatga
     4621 tattttgata cgtatttatc tcaactagcc atgactgttc tggagaaaaa tcaacataag
     4681 cttcatcaat taccacgatt ccatcaaagt tttgtatgat gtgtttcatc tcattgcgat
     4741 tcaacagatt acccgtcgga ttatttggtg aacaaagaaa aataatttta gtatgcttat
     4801 caacagcatt cattaccctt gttgcatcca acgaataatc atcattcaat agcactttac
     4861 ggtattccac attattaaca tcagcacaca ctttgtacat accatacgta ggatcgatag
     4921 ctacaatatt atcgactaag ggttcgcaaa aaatacgaat aactaaatca atgggttcgt
     4981 cgctaccatt gcccagcatt atttgtgatg gattaaccga ttttagtttt gcaatctttt
     5041 cttttaactt ccattgcaag gggtctggat acctattgta cggtgcattc attggatttt
     5101 cgttggcatc taaatataca gatgcttctc cttgaaattc atcacgtgca caggaatacg
     5161 gttttaatgc taagatattt tttctgacta aattttctac gttcatagta aatagataaa
     5221 tgaacaacaa aaatagtaaa tttctatatt ggttgttcat ttcttgaggt attttattca
     5281 ataatcatga agcttcaata tatttaatta ttagtattcg ttcaaaaaga aatagcctag
     5341 caacacatat tgctaggcta ttctttttgt gatccgcctg gggctcgaac ccaggacccc
     5401 atccttaaaa gggatgtgct ctacctgctg agctagcgaa tcttatcttt ttctacaaaa
     5461 gcggtgcaaa gataaagtta tattttttat ttcgcaagta aacaactgct tttttagatt
     5521 ttgatatcac tatatttatg tcctactatt gtcaatcact taataatgtt ggatttatga
     5581 taaataaata attaaaaaaa aatggaaaag gaaagatttt acttcccttt tccatacaca
     5641 cattttcaga acattcttga ttattatttt ttaggggatt tattctctct tgatttttta
     5701 tcttggtttt gtgctcccct atccctaaaa tcttgtagca attcttgttt aaattctcta
     5761 tctgcttgat atacttcaaa caattttgat gccggtaaga tttttttaaa cttctcataa
     5821 tatgcttttt caagattagc tgtttctact ctattttgga taaaattatc cataacagcc
     5881 tccttctctt gctcactcag ctcagtattt tttgctttaa agcctttccc ctgaggtctt
     5941 gatttgcgat taagctccat tctttgctgt tcaaactctt ggtacaacgg aaaaaattca
     6001 gctgattctt tatccgtaaa acttaccttt tctttgataa aagcaatctt ttgtgcttgc
     6061 atttcttgca ttttggctgc tctcatttct gctctattgt ctgtttgggc aactatcata
     6121 gttattccgc ccaaaagcat taaacatgct actactgttt ttttcatatt cattttcatt
     6181 ttttggttta cactggagat gtattttgtg cacaaatatt tctaggttct atattacgta
     6241 ttatgcgata ttctatacta attattatac gataaaagtt catataaagc ataatcatca
     6301 atttcatcta ataataactc tttctgaata gcaagctcca ctaattcttg ttctttttgt
     6361 ttttgttgga tggagggctt gacgaaggta gtgatagata catacaatcc aataaacata
     6421 gctgctaaat acacataagg ttgtacacgt tgccaaaggc ttaagtttat tacttgatta
     6481 ctttcctctt gcttatctag taattgttct atcttagcag aaaattcttc aaaataatta
     6541 tctggcgttg taaatggaac tttattccct acctcatttc tactgttatc atttatttcc
     6601 atactattgt tttctgttac tttgacgatt gatatttcaa atggtttaat ccattagtaa
     6661 aattaatttt t
//
LOCUS       DAOSMY010000037         6662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11582_length_6662_cov_3.730589, whole genome shotgun sequence.
ACCESSION   DAOSMY010000037 DAOSMY010000000
VERSION     DAOSMY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11582_length_6662_cov_3.730589"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLD76_01580"
     CDS             complement(<1..283)
                     /locus_tag="PLD76_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOU26729.1"
                     /translation="MAIIKPFKGIRPPQSIVEQVASRPYDVLNSEEARKEAEGNEKSL
                     YRIIKPEIDFPIGKDEHDADVYEKAAENFQLFQDKGWLVQDDSEKYYVYA"
     gene            complement(373..1293)
                     /locus_tag="PLD76_01585"
     CDS             complement(373..1293)
                     /locus_tag="PLD76_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU26730.1"
                     /translation="MKVLVATDKPFAKVAIDGIKKVVVAAGFELALLEKYTEKQQLLD
                     AVADTNAIIIRSDIVDAEVIAAAKQLKIVVRAGAGYDNVDLEAATAHNICVMNTPGQN
                     ANAVAELALGMMVYGVRNFYNGSSGTELLGKKLGIHAYGNVGRNVARIAKGFGMEIFA
                     YDAFCPAHVIEQDGVTAVQSAAELYKTCQLISLHIPATAETKNSINYELLSTMPKGGM
                     LINTARKEVINEAELIKLMEDRTDFKYLTDIPPSHHDEFTTHFPGRYFSTPKKMGAQT
                     AEANINAGIAAAEQIVNYILHGNDKFRVNK"
     gene            complement(1316..2383)
                     /gene="serC"
                     /locus_tag="PLD76_01590"
     CDS             complement(1316..2383)
                     /gene="serC"
                     /locus_tag="PLD76_01590"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOU26731.1"
                     /translation="MKKHNFNAGPSILPREVIEKTAQAVLDFENDGLSILEISHRAKN
                     FQPIVDEAVALFKELLNIPEGYSVLFLGGGASLQFCMLPFNLLEKKAAYLNTGAWAKK
                     ALKEAKAFGEVVEVASSADANYTFIPKGYSIPTDADYFHITTNNTIYGTELLEDITSP
                     VPLIADMSSDIFSRPVDVSKYGMIYGGAQKNLAPAGVTFVIVKDELLGKVSRYIPTML
                     DYRTHIKEGSMFNTPPVLPIYAALQTLKWLKSQGGLVQVEKNNIAKATLLYNAIDNSK
                     MFVGTANKEDRSRMNICFVMKPEFQHLEADFLKFATSKGMVGLKGHRSVGGFRASCYN
                     ALPIESVQALVDCMNEFETNN"
     gene            complement(2565..3782)
                     /gene="pepT"
                     /locus_tag="PLD76_01595"
     CDS             complement(2565..3782)
                     /gene="pepT"
                     /locus_tag="PLD76_01595"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOU26732.1"
                     /translation="MKSITERFLSYIEIDTQANETSNSTPSSMGQVLFAEMLEKELAS
                     LGLDVIRTDKSYVYATLKSNLTEKKIPTIAFIAHMDTSPAMSGKNIRPNIVKAYDGKD
                     IILQKEGILSPQQFPELLHYIGQDIIVTDGTTLLGSDDKAGIAAIVSAIEKICEDNNF
                     KHGDILFAFTIDEEIGKGADHFDLNLCRADWAYTIDGGEIGELEYENFNAAMATITIQ
                     GNSVHPGYAKNKMINSMLIAQEVISLLKQFGLPENTSGYEGFFHLDKIEGNVEKTILQ
                     YIIRDFDIQDFTNRKANLSTIVRQMNEKYPLACINVLIEDQYYNMKSTIEPIMHIVSL
                     AEQAMIEVGVTPKIKPIRGGTDGARLSYMGLPCPNIFAGGHNFHSKFEYLPISSLEKS
                     MQTILKIIQIVSR"
     gene            complement(3845..4903)
                     /gene="rfbB"
                     /locus_tag="PLD76_01600"
     CDS             complement(3845..4903)
                     /gene="rfbB"
                     /locus_tag="PLD76_01600"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU26733.1"
                     /translation="MEKRTILITGGAGFIGSHVTRLFVNKYPNYTLYNLDALTYAGNL
                     ENLTDIANKPNYNFIKGDITDEIFIQELFQKYQFDGVLHLAAESHVDRSITDPLAFIK
                     TNVLGTANLLTAAKNLWKNNMEGKRFYHISTDEVYGSLGKEGFFTEETPYDPRSPYSA
                     SKASSDHFVRAYYHTYGLPVVISNCSNNYGANHFPEKLIPLAINNIKHSKPIPIYGKG
                     ENIRDWLWVNDHARAIDAIFHTAKIGETYNIGGHNEWTNIDLIKKLCEIMDTKLGRPK
                     GTSAQLITYVSDRAGHDMRYAIDASKLERELGWTPSLQFDEGLEKTVDWYLANEEWLD
                     NVTSGNYQSYYEKQYTDR"
     gene            5060..6031
                     /locus_tag="PLD76_01605"
     CDS             5060..6031
                     /locus_tag="PLD76_01605"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26734.1"
                     /translation="MNTIICYIEELLYEHDFVVIPTFGGFVAGYQSSRITPEGNIYPP
                     QKEIGFNTNVQYNDGLLVQTMMEKQGISFSQASALLHVHIEEWKKELYREKNLHFGNI
                     GSFTLTNTNVISFTPAFTNDFLCSSFGFYPLYLPQLSQELVYNQAPVFVDTVRAFTPE
                     PISWSNTVRYIVASAAVILFFLFFPVQLTNIPIDSQAGVCGYRFTSAVSGNLSVDENE
                     RVEVVDNQQYKDSCIQKVNTLTTLNGVQQLPERFYIVIGSFQTERMAQRFVSELPAIF
                     SSATIVFSENRYRITTASFETEQEGEYFLHTFQQNHPRFKDAWLLKK"
     assembly_gap    6203..6212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6213..>6662
                     /gene="rpsP"
                     /locus_tag="PLD76_01610"
     CDS             <6213..>6662
                     /gene="rpsP"
                     /locus_tag="PLD76_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU26735.1"
                     /translation="SRAPRDGKFIERIGSYNPNTNPATVNVNFEKALKWIETGAQPTD
                     TVRNILSREGVMMKKHLLGGVKKGAFTAEEADKKFDAWMKNKQVAVAKVKEKLASDAS
                     MTAKQRLDAEKAANKAKAAEVAKKQAEKLAVAEPVAEEVATTETETSV"
BASE COUNT         2053 a         1171 c         1135 g         2293 t
ORIGIN      
        1 gagcatatac gtaatatttc tccgaatcat cttgcactag ccatccttta tcttgaaaaa
       61 gttgaaagtt ctcagctgcc ttttcgtata catctgcatc gtgttcatct tttcctattg
      121 gaaagtcaat ttctggttta ataatgcgat acaatgattt ttcgtttcct tctgcttctt
      181 tacgtgcttc ttcggaatta agtacatcat acgggcgaga agccacttgt tctacaatag
      241 actggggtgg acgtatgcct ttaaatggtt taatgattgc cataatcttt tttactattt
      301 gtttgataaa ttaatctaat tcaatgatat atataaagaa aaagcaccta ttcgtagatg
      361 ctttttttat cgttatttat ttacacgaaa tttatcattt ccatgtaaaa tataattgac
      421 tatttgttct gcagcagcta taccagcatt gatgtttgct tcggctgttt gagcacccat
      481 ttttttggga gtagaaaaat atctacctgg aaaatgggtc gtaaattcat cgtgatgcga
      541 cggcggaata tcggtaagat atttaaaatc agtcctgtct tccattaatt ttatcaattc
      601 tgcctcgttg ataacttctt ttcttgctgt attgattagc ataccacctt taggcattgt
      661 agaaagtaat tcataattga tagagttttt tgtctcagca gtagcaggta tgtgtaagga
      721 aataagctga catgttttgt atagttctgc cgcagactgt acagcagtta ccccgtcttg
      781 ttcaataacg tgtgcaggac aaaatgcatc gtaagcaaaa atttccatgc caaatccttt
      841 ggcaatacgt gctacatttc taccaacatt tccatatgca tgtatgccta atttttttcc
      901 taataattca gtacccgaag atccattgta aaaattacgt actccgtata ccatcattcc
      961 aagagctaat tcggctacgg cattggcatt ttgaccaggc gtattcatta cacaaatatt
     1021 atgtgctgtg gctgcttcca aatcaacatt gtcgtatcca gcaccagctc ttacaactat
     1081 ttttagttgt ttggctgcag caattacttc cgcatctaca atatcactac gaatgataat
     1141 tgcgttagta tctgctactg catctaataa ttgttgtttt tctgtgtatt tttctaacaa
     1201 ggctagctca aatcctgctg ctaccactac ttttttaata ccgtcaattg ctactttggc
     1261 aaacggttta tctgtcgcta ctaatacctt catgtttcga taaaattatg tatgtttagt
     1321 tattggtttc aaattcattc atgcagtcaa ctaaagcttg tacactttct attggcaatg
     1381 cattgtaaca tgatgcacgg aaaccaccta ccgaacgatg accttttaat ccaaccatac
     1441 cctttgaggt agcaaatttt aagaagtctg cctctaaatg ttggaactct ggtttcataa
     1501 caaaacaaat attcatacga gatctatctt ctttgtttgc tgtgcctaca aacattttac
     1561 tgttgtcaat agcattgtat aacaaagtag cttttgcaat gttatttttt tctacttgta
     1621 ctaaacctcc ttgcgatttt aaccatttta atgtttgcaa agccgcatat ataggtaata
     1681 ctggaggagt attgaacatt gatccctctt ttatatgtgt tctataatct agcatggtag
     1741 ggatataacg gcttactttg ccaagaagtt cgtcttttac aataacgaaa gtaacaccag
     1801 ctggggctag atttttttgt gcaccaccgt atatcatacc atattttgat acatctactg
     1861 gacgagaaaa aatatcagaa gacatgtctg ctatgagggg tactggagaa gtaatatctt
     1921 ctaataattc agtaccataa attgtgttgt ttgtagtaat gtgaaagtaa tcagcatctg
     1981 ttggaatgct atatccttta ggaataaatg tataattagc atcagcagaa cttgcaactt
     2041 ctactacttc tccaaaggct tttgcttctt ttagtgcttt tttagcccat gcacctgtat
     2101 tcaaataagc tgcttttttc tctaataaat tgaatggaag catacaaaat tgcaaactag
     2161 caccgcctcc caaaaataat accgaatagc cttcaggtat atttaataat tctttgaaca
     2221 atgcaacagc ttcgtctaca atgggctgaa agttcttagc tctatggcta atttccaaaa
     2281 tagacaaccc gtcattctca aaatctaata cagcctgtgc tgtcttttca atcacttctc
     2341 ttggtaagat agatggtcct gcgttaaaat tatgcttttt cattcgaaaa aataaattaa
     2401 attagtttgt aaataatacc tacacgtagg ttttttataa ttaattagat tgtaaaaatt
     2461 tgcgtttaaa atcttcctgt tagctcagac tttttagaaa gcacaaaatt attgtttttt
     2521 ttcctatctt gtatcaaaaa gagaaggtat ttttacacaa gatattaacg acttacaatt
     2581 tgaataattt taagtatagt ctgcatcgat ttttctaaag aagatatagg taaatactca
     2641 aatttactat gaaaattgtg tccgccagca aaaatgttgg gacagggtag ccccatgtac
     2701 gaaagacgag caccatccgt ccctccacgt attggtttta tttttggtgt tacacctact
     2761 tctatcattg cttgttccgc taagcttact atatgcataa taggttcaat agtggatttc
     2821 atattgtaat actgatcttc aattaaaacg tttatgcaag ctaagggata tttttcattc
     2881 atttgtcgta ctatagtgga taaatttgct tttctatttg taaaatcttg tatatcaaaa
     2941 tcacgaataa tatactgtaa aatagttttt tccacatttc cttcaatttt atctaaatgg
     3001 aaaaatccct cataaccact tgtgttttca ggtaatccaa attgtttgag caatgaaata
     3061 acttcttgtg caatgagcat agagttaatc attttatttt tcgcataacc cggatgtacg
     3121 ctgtttcctt gaatggtaat tgtagccatt gcagcattaa aattttcata ttctaattcg
     3181 ccaatctccc caccgtcaat tgtatatgcc caatctgctc gacataaatt taagtcaaaa
     3241 tgatctgccc cttttccaat ttcttcatca atagtaaatg cgaataaaat atctccatgt
     3301 ttgaagttat tgtcttcgca aattttttct atagcactaa caatagctgc aatacctgct
     3361 ttatcatcgc ttcctagcaa ggtggtgcca tcggttacta ttatgtcttg tccaatatag
     3421 tgcaggagct caggaaattg ttgaggggat aagattcctt ctttttgtag tatgatatct
     3481 tttccatcat acgcttttac aatgttgggt cgtatgtttt tcccactcat tgctggactt
     3541 gtatccatat gtgcaataaa agctatagta ggaatttttt tctcggtcaa gttacttttg
     3601 agggtagcat acacatatga tttatctgtt cgtattacgt ctaatcccaa tgatgctaat
     3661 tctttttcaa gcatttcagc aaatagtact tgacccatgg aactaggggt tgagtttgat
     3721 gtttcattag cttgtgtatc aatctcaata taagataaaa aacgttcagt gatgcttttc
     3781 attttgtagt atactaaaaa gaggagtgtg cacacactcc tctttgatga ttgaaatttg
     3841 tatgttatct atctgtatat tgtttttcgt aatagctttg gtaatttcca ctcgttacat
     3901 tatcaagcca ttcttcgttt gctaagtacc aatctactgt tttttctaat ccttcatcaa
     3961 actgcaaaga aggagtccat cctaattcac gttctaattt gcttgcatca atagcataac
     4021 gcatatcatg accagctcta tctgaaacgt acgtaattag ttgggctgat gttccttttg
     4081 gacgaccaag ttttgtatcc atgatttcgc acaacttttt aatcaaatca atgtttgtcc
     4141 actcattgtg tccaccaatg ttatatgttt caccaatttt agcagtatga aaaatagcat
     4201 ctatcgcacg tgcatgatcg tttacccaca accaatcacg gatattttct ccttttccgt
     4261 aaatgggaat tggtttgcta tgttttatat tattgatagc tagtggaatt aatttttctg
     4321 ggaagtgatt cgctccatag ttatttgagc aattggaaat tactacaggt aacccatagg
     4381 tgtgataata ggcacgaaca aagtggtcag aactagcttt agaagcagaa taggggctgc
     4441 gagggtcgta aggagtttct tctgtgaaaa aaccttcttt acctagtgag ccatacactt
     4501 catcggttga aatatggtaa aaacgtttcc cctccatgtt gtttttccac aaatttttag
     4561 cagctgttaa tagattagca gtacccaata cattggtttt aataaaagct aatgggtcgg
     4621 taattgaacg gtcaacgtga gattcagcag caaggtgcaa tacaccatca aattggtatt
     4681 tttggaataa ttcttggata aatatttcgt cggtaatatc cccttttata aaattgtaat
     4741 ttggtttatt ggctatgtca gttaagtttt ccaaattacc agcatacgta agagcatcta
     4801 aattatacag tgtgtaattt ggatacttgt ttacaaataa gcgtgttacg tgagacccaa
     4861 taaaaccagc acctcctgta atcaaaattg ttcttttttc catttaaaaa aatgtattag
     4921 gatttaacgg gtacaaaatt acataaaaag acgtatatta caacaaattg tacagaatat
     4981 tttattgttt ataagttgtt caaaactttt aatttatcta tattgcatag tagcattatt
     5041 attacgtact ttgtcggcta tgaatacgat tatatgttat atagaagaat tattgtacga
     5101 acacgatttt gtggttatac ccacatttgg aggttttgtg gctggttatc agtcttctag
     5161 gataactcca gagggaaata tttatcctcc tcaaaaagaa ataggtttta atacaaatgt
     5221 acaatataat gatgggttgc tggtgcaaac aatgatggaa aagcaaggta tcagtttttc
     5281 tcaagctagt gctttacttc atgtacatat tgaagaatgg aaaaaagagt tgtatagaga
     5341 aaaaaacttg cattttggaa acataggcag ttttacacta acaaacacga acgttatctc
     5401 ttttacccca gcctttacca atgatttttt gtgttcttct tttggttttt accctttata
     5461 tttaccacag ctatctcagg aattagttta taatcaagca ccagtttttg ttgatactgt
     5521 tcgtgcattt actccagaac ccatttcttg gtcgaatacg gttagatata tagtagcaag
     5581 tgccgctgta attttatttt ttctgttttt tcccgttcaa ttaaccaata ttcctattga
     5641 ttctcaggct ggtgtatgtg gataccggtt tacctcagca gtgtctggaa atttgtctgt
     5701 ggatgaaaat gagagagttg aagttgttga taatcagcaa tataaagaca gttgtattca
     5761 aaaagtgaat acgctgacta ctttaaatgg tgtacaacaa ctaccagaac gtttttatat
     5821 cgttataggg agttttcaaa cagaacgaat ggctcaacga tttgtgtcag aattgcctgc
     5881 catattcagt tctgctacta ttgtgttttc tgaaaaccga tatcgtataa ccacagcaag
     5941 ttttgaaaca gagcaagagg gagaatattt tcttcacact tttcagcaaa atcatccgcg
     6001 atttaaagat gcttggttgc ttaaaaaata aattttccat aaaaaaactg ccgataagta
     6061 tttgtcagct taataattag tagtatcttt gcatccgcat tcgggaaatg cacagaagtt
     6121 tattaactaa taaaaaaaac aaaaaagtat ggcaactaaa attagattgc aaagacatgg
     6181 acgcaaaggg tatgcttatt acnnnnnnnn nncagcaggg caccacgtga tggtaagttt
     6241 attgaaagga taggttcgta taacccgaac acaaatcctg ctacagtaaa tgtaaacttc
     6301 gaaaaagcct taaaatggat tgaaactggt gctcaaccaa cagacactgt aagaaatatt
     6361 ttatccagag aaggtgtaat gatgaaaaaa cacttattgg gtggtgtaaa aaaaggtgca
     6421 tttacagcag aagaagcaga caagaagttt gatgcttgga tgaaaaataa acaagtagct
     6481 gtagcaaaag tgaaagaaaa attagctagt gatgcttcaa tgacagcaaa acaacgtttg
     6541 gatgcagaaa aggcagcaaa taaagcaaaa gcagctgaag tagcaaaaaa acaagcagaa
     6601 aaattggctg tagctgagcc tgtagccgaa gaagtagcaa ctacagaaac agaaacctct
     6661 gt
//
LOCUS       DAOSMY010000038         6614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11700_length_6614_cov_3.227931, whole genome shotgun sequence.
ACCESSION   DAOSMY010000038 DAOSMY010000000
VERSION     DAOSMY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11700_length_6614_cov_3.227931"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLD76_01615"
     CDS             <1..338
                     /locus_tag="PLD76_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU26736.1"
                     /translation="RKKRNAIALEYLEKMGLKDWAHHYPNELSGGQKQRVAIARALIS
                     NPRVILADEPTGALDSQTSVEVIDLLKAINQQGVTVIVVTHENEVAASTERIIRMKDG
                     IIQPQDIHQ"
     gene            342..1607
                     /locus_tag="PLD76_01620"
     CDS             342..1607
                     /locus_tag="PLD76_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU26737.1"
                     /translation="MIHFQLWEEIFASLRRNKLKTALTGFAVAWGIFMLIVLLAAGNG
                     LKNGMALNYKNDSKLAVWMWSGRTNLAYKGLPTKRNIQFTNTNETILRKDFDASIGEL
                     SPLIDMRGKNVMYKNEKGIHDVRGVLPEFVPIEALEMLPNQGRFVNQLDCDHKRKVVV
                     VSSKFVQDFFKDESPLGKQLSIDGIMFTVIGVFKEVPRSWQKLVYIPLPTAQSLYGFG
                     DKIHEISFNLHMQNETNVDESKQIMDNIKQRFASYHQFDVEDEGAMGYWNTLESFLFT
                     QKLFAGISIFVWIIGVGTLIAGIVGISNIMLISVKERTKEFGIRRALGAKPSSIIVLV
                     VLESIIITAFFGYIGMVFGVGLTEGVNYVMKQMPMDETGAVFFYNPTIQLDLVMVATV
                     LLIIAGVIAGYFPARKAIRIKPIDALRDE"
     gene            1612..2868
                     /locus_tag="PLD76_01625"
     CDS             1612..2868
                     /locus_tag="PLD76_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU26738.1"
                     /translation="MRIVDIDRWQEIWHTIKQNKLRSVMTAFGVFWGILMLVIMLGSG
                     KALENGILDGTENFERNSCFMFAGTTTEPYKGFQRGRSWYITSSDLVAIEKNVEGVKF
                     TVPYVFPTNTTENNVMNGMKVGSFSVQGITPELYLVNPQILLQGHLINKLDELGKRKI
                     CVIGEKVKEQLFLADETVVGTYIRVNGVYFQVVGVIKSATQMNIGSQPENTVFVPYTT
                     AKQVFNLGDRISYISVVAEDWADISKVEEDTKEVLKARNSIAPSDNEAIWSFNMKQQF
                     QQIGALLLGIQLLIWIVGIGTLLAGIIGVSNIMLITIRERTKEIGIRRAIGATPKVII
                     GQILVESLVLTVLAGMGGLVLGVWILHFANGLIPADGMFFKNPHIDFGVAIIATGIMS
                     LFGVLAGIIPARRAIRIKPIDAIREE"
     gene            2896..3978
                     /locus_tag="PLD76_01630"
     CDS             2896..3978
                     /locus_tag="PLD76_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU26739.1"
                     /translation="MKKFLKISAIVILVVLVLGTFYMLWKKSQPEKTSFSTTSPIVTT
                     IENKTVATGKVEPRTVVQIKPQISGIITEIYKEAGQQVKAGDVIAKVQIIPDMVSLSA
                     AESRIKRAQIALEKAQIDYDRQKSLFDKGVISQEAYQTATIELKSSQVDLVDAQNNYD
                     LMKEGITRNASNYSNTLIRSTINGMILDIPQKVGSSVIQSNNFNDGTTIATVANMKDM
                     IFVGKIDETEVGKIKVGMPIVLVIGALEGKTFDATLEYIAPQGISDGGAIVFEIKAAV
                     SLSDDVFVRSGYSSNAQIILEKKENILALPESALIFEKDSVFVEVQTKDTLVFDKRAV
                     KLGLSDGINIEVLEGVTQNEKIKLVK"
     gene            3983..5287
                     /locus_tag="PLD76_01635"
     CDS             3983..5287
                     /locus_tag="PLD76_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU26740.1"
                     /translation="MRTPLLVIWLLFVSSILFGQQKWTLEQCLEHAKVENIQVQQRLI
                     QAENAKINLQQSKQNLLPDLNMYVGQSFSFGRSIDETNTYQNNNVQNTNFSLSSSLNL
                     YDGGRMFYAIEKSKLDFQALLLDVEQEKSTIELQVLLHYFQVSYAKKQVELAENQYAL
                     TSNQLEKTKALIDAGRLPEGEIYQVKAQLSQEELAILQAKHEVNNLLMQFAQLLLIDD
                     VDSFDIEDVEIVAREEYLLPMFENVYTSTIATMPSIRAEQLRLESANQQVRIEKSAYY
                     PRLSMGASYGNGYYNAENRLNLPFSEQWQQNQNIAVTLNLQIPIFNKMSVINRVKQSE
                     LAVKSQELVAKNTKIQLRQELQQAYLLTVAAKEKISVSENNVRAMQKSFDYQTEKFDS
                     GVSTIYEYNEAKNALTKALSNQTQAMFEFLFRSKAIELYMGK"
     gene            complement(5291..5812)
                     /locus_tag="PLD76_01640"
     CDS             complement(5291..5812)
                     /locus_tag="PLD76_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU26741.1"
                     /translation="MIYLELFWSFFTIGLFGFGGGYSILSLIQHEVVDIQGWLTIADF
                     TNIVAISQVTPGPIAINCATYVGYTATNSILGSAVATIAVSLPSLFIMVLISRFINAF
                     RSNPYVAAALNTLKPAIIGLIASAALLLSNPSNFIDYISFIIFGTVFVAAYCKVNPIF
                     LILLAGMAGLVIY"
     gene            complement(5809..6354)
                     /locus_tag="PLD76_01645"
     CDS             complement(5809..6354)
                     /locus_tag="PLD76_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU26742.1"
                     /translation="MNAWKLFSTFFKIGTFTFGGGYAMLPLIEQEVVHKQQWLNTEEY
                     TEMLSLVQTAPGPISINSAVFIGYKLHRWKGALSCLLGVILPSFIIILLIAVAFTEIN
                     SNPIVERIFKGIRPAVVALIAVPVIRMIQREKLHWTSGLIALLSIILVWLLGYSPVWV
                     VLGAIIYGIVKEYLTTKRKQP"
     gene            complement(6415..>6614)
                     /locus_tag="PLD76_01650"
     CDS             complement(6415..>6614)
                     /locus_tag="PLD76_01650"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOU26743.1"
                     /translation="PNGSDYNVAGICSADGRHLAMMPHLERAIFPWQWAYYPIDRKTT
                     DEVSPWIEAFVNAKNWILSHK"
BASE COUNT         2214 a          968 c         1396 g         2036 t
ORIGIN      
        1 ctcggaaaaa gcgtaatgct atagcattgg agtatttgga aaaaatgggt ttgaaagatt
       61 gggctcatca ttatcctaat gagctttctg ggggacaaaa acagcgtgta gccattgctc
      121 gtgcacttat ttctaatcca agagtaatat tagctgacga accgactgga gccttggata
      181 gtcagacttc agtagaagtt atagacttat taaaggcaat taaccaacag ggtgtaaccg
      241 tgatagtggt aactcacgaa aatgaagtag ctgcctcaac agaacgtatt atacgaatga
      301 aagatggtat tattcagcct caagatatac atcagtaaga tatgatacac tttcaattat
      361 gggaagaaat ttttgcgtct ttgcgtagaa ataaacttaa aacagcttta actggatttg
      421 ctgtggcatg gggtatattt atgctcattg ttttgctggc agcaggtaat ggcttaaaga
      481 atggaatggc attgaattat aaaaatgact ccaagttggc tgtttggatg tggtctggta
      541 gaactaattt ggcatacaaa gggcttccta caaaacgcaa tattcagttt acgaatacga
      601 acgaaacaat attgaggaaa gattttgatg catctattgg agaattatcg cccctgattg
      661 atatgcgagg gaaaaatgtg atgtacaaaa atgaaaaagg aattcacgat gtgcgtggtg
      721 ttttgcccga gtttgtgcct attgaagcat tggagatgct ccccaatcaa ggacgttttg
      781 ttaaccaatt ggattgtgat cataaacgaa aagtagtggt tgtaagttcc aaatttgttc
      841 aggatttttt taaagacgag tccccgttag gtaaacaatt gagtatagat ggtatcatgt
      901 ttaccgttat aggcgttttt aaagaagtgc ctcgtagctg gcaaaaatta gtgtatattc
      961 cacttcctac agctcaaagt ttgtatggat ttggagataa gatacatgaa atatcgttta
     1021 atttacatat gcaaaacgaa accaatgttg atgaaagcaa gcaaattatg gataatatta
     1081 agcaacgttt tgcatcatac catcaatttg atgtagaaga tgaaggagcg atggggtatt
     1141 ggaataccct agagagtttt ttgtttaccc aaaagttatt tgctgggata tctatttttg
     1201 tgtggattat cggtgttgga actcttattg cgggaattgt tggtattagt aatatcatgt
     1261 taataagcgt aaaagagaga acaaaagaat ttggtattcg acgagcttta ggagctaaac
     1321 catcttcaat tattgtgtta gttgtgttag aatcgattat tataacggct ttttttggtt
     1381 atatcggaat ggtgtttggt gttggtctga ccgagggagt aaattatgtg atgaagcaaa
     1441 tgccaatgga tgaaacaggt gcagtctttt tttataaccc cactattcaa cttgatttag
     1501 ttatggtggc tactgttttg ctcattattg caggtgttat tgcaggttat tttcctgcac
     1561 gtaaggctat tagaattaaa cctatagatg ctttgagaga cgaataagta tatgcgtata
     1621 gttgatattg acagatggca agaaatttgg catactataa agcaaaataa gcttcgtagt
     1681 gtaatgactg cgtttggtgt gttttgggga atactgatgt tggttattat gcttggttct
     1741 ggcaaggctt tggagaatgg gatattagat ggaactgaaa attttgaaag aaattcatgt
     1801 tttatgtttg ctgggactac tactgaacca tataaggggt ttcagcgtgg acgttcgtgg
     1861 tatattacat cgagtgattt ggtggcgatt gaaaaaaatg tagagggagt taaatttact
     1921 gtcccttacg tttttccaac aaatactacg gagaataatg tgatgaatgg tatgaaagtg
     1981 ggatctttct ctgtacaggg aattactccg gaattgtatt tggtgaatcc tcagattttg
     2041 ttgcaaggac atctcattaa taaattagat gaactaggca aacgaaaaat ttgtgttata
     2101 ggggaaaagg taaaagaaca acttttttta gcagatgaaa cagttgtggg cacatatatt
     2161 cgtgtaaatg gtgtgtattt tcaggtagtg ggagttatta aatcagcaac tcaaatgaat
     2221 attggtagtc agccagaaaa taccgtattt gtaccatata ctactgccaa acaagtattt
     2281 aatttaggag atcgaatatc atatatctct gtagttgccg aagattgggc agatatatcc
     2341 aaagtagaag aagatactaa agaggtactt aaggctcgta atagtatcgc tccgtctgat
     2401 aatgaagcta tttggagttt taatatgaaa caacaatttc aacaaattgg agcgttattg
     2461 ctcggtattc aactattaat ttggatagtg ggaattggta ccttgttagc tggtataata
     2521 ggtgtgagca atattatgct tattaccatt agagagagaa caaaagaaat tggaattagg
     2581 cgagccattg gagccactcc caaagttatt attggtcaaa ttttggtaga aagtttggta
     2641 cttactgtat tagcaggtat gggaggctta gttctgggtg tgtggatatt gcattttgcc
     2701 aatggattaa ttcctgctga tggcatgttt tttaagaatc cacatatcga ttttggagtt
     2761 gcgattatcg ctacaggtat tatgtcgcta tttggtgtgc ttgcaggaat tattcctgcc
     2821 cgaagagcta ttcggataaa acccattgat gcaataagag aagaataaaa tgtaacattt
     2881 taactataaa aagatatgaa aaagttttta aaaattagtg caattgtcat attagttgtg
     2941 ttagtattgg gcacgttcta catgttgtgg aaaaaatcac agccagagaa aactagcttt
     3001 tcaacaacat ctccgattgt taccactatt gaaaataaaa ccgtagcaac aggaaaggtt
     3061 gagccacgta cggttgttca aattaaacct caaatatcag gtattattac tgaaatatac
     3121 aaagaggctg ggcaacaagt gaaagcaggt gatgtaatag ctaaggtaca aattattcct
     3181 gatatggtgt cattaagtgc tgccgaatcg cgtattaaaa gggctcaaat agcacttgaa
     3241 aaggctcaaa tagattatga tagacaaaaa tctttgtttg ataagggggt tatttctcaa
     3301 gaagcttatc aaacagctac tatcgaactt aaatctagtc aagtagactt agtggatgct
     3361 caaaataatt acgatttgat gaaagaaggc ataacacgta atgcaagtaa ttacagtaat
     3421 acacttattc gttccacaat caatggcatg attttagata ttcctcaaaa ggtaggtagt
     3481 tctgttattc aaagtaataa ttttaatgat ggcaccacta ttgctacagt tgccaatatg
     3541 aaagatatga tttttgtagg aaagattgac gaaactgaag tgggtaaaat aaaggttgga
     3601 atgcctattg tgctagttat tggagcttta gaaggtaaaa catttgatgc tacattagag
     3661 tatattgcac cacaagggat aagcgatggt ggagctattg tatttgagat taaagcggct
     3721 gtgtcattgt ctgatgatgt ttttgttcgg tcggggtata gttctaatgc acagattata
     3781 ttagaaaaga aagaaaatat tctggctttg cccgaaagtg ctttgatttt tgagaaagat
     3841 agtgtatttg ttgaggttca aacaaaagat acattagtat ttgataaaag agctgtcaaa
     3901 ttaggtttat ctgatggtat taatattgaa gtgttggaag gcgtaacgca aaatgaaaag
     3961 attaaacttg ttaaatagaa atatgagaac accactatta gttatatggt tgctattcgt
     4021 tagtagtata ctatttggtc agcaaaaatg gactcttgag caatgtttag agcatgcaaa
     4081 agtagaaaat attcaagttc aacagcgatt gatacaggca gaaaatgcaa aaattaatct
     4141 acagcaatcg aaacaaaatt tattacccga tttaaatatg tatgtaggtc aaagctttag
     4201 ttttggtcga tctattgatg aaacaaacac gtatcaaaat aataatgtcc agaatacaaa
     4261 tttttcatta tcaagtagtt taaatttgta tgatggtggt aggatgtttt atgctattga
     4321 gaaatcaaaa cttgattttc aggcactatt actagatgta gaacaagaaa aaagtacgat
     4381 tgaattacaa gttcttttgc actattttca ggtgtcatat gcaaagaaac aggtagaatt
     4441 agctgaaaat caatatgctt tgactagcaa tcaacttgaa aaaacgaaag ctttaataga
     4501 cgcaggaagg ttgcctgaag gcgaaatata ccaagtaaag gctcaattat cgcaagaaga
     4561 acttgctata ttacaagcaa agcatgaagt taataatttg ttgatgcagt ttgctcaact
     4621 ccttttaatt gatgatgtgg atagctttga tattgaagat gtagagattg tggcaagaga
     4681 ggagtatttg cttccgatgt ttgaaaatgt atatacaagt acgattgcta ccatgccttc
     4741 tataagggcg gaacagcttc gattagagag tgctaatcaa caagtacgta tagaaaaatc
     4801 ggcttattac ccacgattat ctatgggtgc tagttatggc aatgggtatt ataatgctga
     4861 aaacaggctt aatttacctt tttcagaaca atggcaacaa aaccaaaata ttgcagtaac
     4921 gctgaatctt caaataccta ttttcaataa aatgtctgtg attaacagag taaaacaatc
     4981 agaacttgcc gtaaagagtc aggaattagt tgctaaaaac actaaaatac aacttcgaca
     5041 agagttacag caggcttatt tgttaactgt ggcggcaaaa gaaaaaatca gtgtgtctga
     5101 aaataatgtc cgtgcaatgc aaaaatcatt tgattatcag acagaaaaat ttgatagtgg
     5161 agtctccact atttatgaat ataatgaggc taagaatgca ttaaccaagg ctttatccaa
     5221 tcaaactcaa gccatgtttg agtttttatt cagaagcaaa gctattgagt tgtatatggg
     5281 gaagtaaatt ttaatatata accaatccag ccatcccagc cagaaggatt aaaaaaattg
     5341 gattgacttt acaataagct gccacgaata cggtaccaaa aatgataaaa ctgatgtaat
     5401 caataaagtt ggagggatta ctaagcaata aggctgctga ggcaattaac ccaataatgg
     5461 ctggttttaa tgtgtttaag gctgctgcta catacggatt gcttctaaaa gcattgataa
     5521 atcggctaat taaaaccata ataaatagtg agggtagcga tacagcaatg gtggcaacag
     5581 ccgatccgag tatcgaattg gtggctgtgt aaccaacata ggtggcacaa ttaatggcaa
     5641 tgggtccagg ggttacttgt gaaatagcta ctatattggt aaagtcggca attgttaacc
     5701 atccttgtat atcaactact tcgtgttgga taagcgataa tatagaatag ccgccaccaa
     5761 aaccaaagag tccgatggtg aaaaagctcc aaaataattc taaataaatc atggctgttt
     5821 tctttttgtg gtaaggtatt cttttacaat gccatagata attgctccta gtacaaccca
     5881 cacaggtgaa taacctaaca accatactag tataatgcta aggagggcaa taagtccact
     5941 agtccagtgg agtttttcgc gttgtatcat cctaattaca ggcacagcta tcaaggctac
     6001 tacagcaggt cggataccct taaatatgcg ttctacaatc gggtttgaat ttatttcagt
     6061 aaaggcaacc gcaattagta aaataataat aaacgaaggc agaataacgc caagcaaaca
     6121 agatagggct cctttccaac gatgaagttt gtagccaata aataccgcac tattgataga
     6181 aattggtcca ggtgcagttt gaaccaaaga taacatttcg gtatattctt ctgtgtttag
     6241 ccattgttgc ttgtgtacca cttcttgttc tattaatggc aacatggcat atcctccacc
     6301 aaaggtaaaa gtgccaattt tgaaaaatgt actaaatagt ttccaagcgt tcatctcgta
     6361 aattctacta taaacaaaag acagtatcat catgtactgt ctttgtcttt ttttttattt
     6421 atgtgataaa atccaatttt ttgcatttac aaaagcttct atccaaggcg atacctcatc
     6481 agtcgttttt ctgtcgatag ggtagtatgc ccattgccaa gggaaaatag ctctttccaa
     6541 gtgaggcatc atagccaaat gtcgtccatc ggcagagcag atacctgcta cattgtaatc
     6601 agaaccattc ggat
//
LOCUS       DAOSMY010000039         6585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11776_length_6585_cov_3.459265, whole genome shotgun sequence.
ACCESSION   DAOSMY010000039 DAOSMY010000000
VERSION     DAOSMY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11776_length_6585_cov_3.459265"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLD76_01655"
     CDS             <1..621
                     /locus_tag="PLD76_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOU26744.1"
                     /translation="DVIMVAPKGSGTSLRRMFLQGRGLNSSYAIFQDATGKAFERVVA
                     LGIGVGSGYLFETTFKKEVYSDLTGERGTLMGAIQGILLAQYEVLRENGHSPSEAFNE
                     TVEELTQSLMPLFAENGMDWMYANCSTTAQRGALDWMTPFHDATKPVFKKLYEEVACG
                     NEAQRSIDTNSKPDYREGLEKELKALRESEMWQAGAVVRKLRPENK"
     gene            775..1794
                     /gene="recA"
                     /locus_tag="PLD76_01660"
     CDS             775..1794
                     /gene="recA"
                     /locus_tag="PLD76_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU26745.1"
                     /translation="MATKDTENQPKVNVEKLKALQLALDKIEKDHGKGTIMKMGDKTV
                     VEVNTISTGSIGLNVALGVGGFPRGRVIEIFGPESSGKTTLAIHAIAQAQKAGGIAAI
                     IDAEHAFDRFYAEKLGVDINNLLISQPDNGEQALEIADQLIRSSAVDIVVIDSVAALT
                     PKAEIEGDMGDSKMGLQARLMSQALRKLTANINRTNTTCIFINQLRDKIGITFGNPET
                     TTGGNALKFYASIRLDIRRIGQIKDGDEVIGNQTRVKIVKNKVAPPFRKAEFDIMFGE
                     GISIAGEIIDLGVEQGIIKKSGSWFSYGDTKLGQGRDAAKKVMIDNPELAAELEQKIM
                     EALAK"
     gene            1832..2569
                     /gene="rlmB"
                     /locus_tag="PLD76_01665"
     CDS             1832..2569
                     /gene="rlmB"
                     /locus_tag="PLD76_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU26746.1"
                     /translation="MKKESEMIYGMHAIIEAIDAGKEIDKIIMKNDMQGRLSHDLFKA
                     IEGRNIPIMRVPVERLDRITKKNHQGVIAFTIPVAYQHIEDIIPTLYEDGKAPFIIVL
                     DGITDVRNFGAIARTCECAGVHAIVIPEKNSASINADAIKTSAGALLKIPVCREKNLT
                     NAIKFLKNSGLKIVATSDKADDNYTDISYTDALAIVMGAEDIGISIENQRLCDTIVKI
                     PIVGTISSLNVSVATGIMAYEVLKQRL"
     gene            2574..3257
                     /locus_tag="PLD76_01670"
     CDS             2574..3257
                     /locus_tag="PLD76_01670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU26747.1"
                     /translation="MNVLFEDNHIIAINKSCSDIVQGDKTGDTPLVEKVKIYLKEKYN
                     KPGNVFCGVTHRLDRPVSGIVLFAKTSKALTRLNEMLREREIKKTYWAITKNRPAKPE
                     MLLEHYLLRNEKQNKSYAYDTPKGDAKKATLTIKELATSTNYTLLEVDLQTGRHHQIR
                     CQLAHIGCPIKGDLKYGFARSNPDGGISLHARQINFIHPVTKEPIEIIAPTPEDNLWK
                     AFEDMIRNA"
     gene            3250..3537
                     /locus_tag="PLD76_01675"
     CDS             3250..3537
                     /locus_tag="PLD76_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOU26748.1"
                     /translation="MHKTILSGIVHEIPEDIAKILLENDAILDKWNALTPIQRNEWIC
                     WVTIVKKEQTRAEHIIRMMDELKAGKGKPCCWPGCPHRRSSAQKWFTNKSM"
     gene            complement(3606..5735)
                     /gene="scpA"
                     /locus_tag="PLD76_01680"
     CDS             complement(3606..5735)
                     /gene="scpA"
                     /locus_tag="PLD76_01680"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOU26749.1"
                     /translation="MKPDFKSIDITNIAVSSGVGTQAESTWVTPEQIPVKPFYTKQDL
                     NGMQHLQYAAGLPPYLRGPYSVMYAMQPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDADHERVVGDVGKAGVSICSIEDMKVLFDGIPLNKMSVSMTMNGAV
                     LPVLAFYINAGLEQGAKLEELSGTIQNDILKEFMVRNTYIYPPKFSMKIIADIFEYTS
                     KNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRAGVNAGMDIDSFAPRLSFF
                     WAIGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNV
                     GRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQQETDICREVDPW
                     AGSYYVESLTNELAEKAWALIEEVEKLGGMAAAIETGIPKMRIEEAAARTQARIDSGQ
                     QTIVGTNKYRLEKEDPLDILEVDNTAVRIQQIERLTQLKQARDNAAVKNALAAITKCV
                     ETGEGNLLELAVEAARVRATLGEISDACEVIVGRYKATIRTITGVYSAGTKNDADFIK
                     ACELTAKFAEKEGRQPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEA
                     AKQAVENDVHIMGVSSLAAGHKTLVPQVIAELKALGREDIIVIAGGVIPAQDYDYLYQ
                     AGVAAIFGPGTSVAKAAVTIMEILLED"
     gene            complement(5812..6426)
                     /locus_tag="PLD76_01685"
     CDS             complement(5812..6426)
                     /locus_tag="PLD76_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOU26750.1"
                     /translation="MKKVLLASLLAAVLWFIMFSPWTSIHINFWIAMACSAAILWAMS
                     AFLDKNFSKQFSFNVKDLVIGLVSAAVLYGVFVVGNYLSTQWFDFAGTQIQGIYGMKE
                     GQNLVLVGVLLTVLIGPAEEIFWRGYVQKQLGAKYGDWFAVLITVLIYTLVHIWSFNF
                     MLIMAAMVCGLFWGILYKYHKNIVTLIVSHAVWDVAVFILIPIA"
BASE COUNT         2206 a         1303 c         1178 g         1898 t
ORIGIN      
        1 gatgttatca tggttgctcc taaaggttca ggaacatcac ttcgtcgtat gttccttcaa
       61 ggacgtggat taaattcatc ttacgcaata ttccaagatg ctacaggcaa agcttttgaa
      121 cgtgttgtag ctttaggtat tggtgtaggt tctggttact tgtttgaaac tactttcaaa
      181 aaagaagtat attctgattt aactggcgaa cgtggtacat taatgggtgc catccaaggt
      241 attttgttag ctcaatatga agtgttacgc gaaaacggac attctccttc tgaagcgttt
      301 aacgaaacag tagaagaatt aactcaatca ttaatgccat tgtttgcaga aaacggaatg
      361 gactggatgt atgctaactg ctcaactaca gctcaacgtg gtgccttaga ttggatgact
      421 cctttccacg atgctactaa acctgtattc aaaaaattat acgaagaagt tgcttgtggt
      481 aacgaagctc aacgctctat cgatacaaac tctaaacctg attaccgcga aggtttagaa
      541 aaagaactta aagcacttcg cgaaagcgaa atgtggcaag caggtgctgt agtacgtaaa
      601 ttgcgtccag aaaataaata ataacgcatt taattgcact ttttaaaagc ggacactatc
      661 caaaagatat ttgtccgctt tttttgttca taaacttttc aacaaactaa caaaatgaat
      721 tggctattga caaaaaaaat tatacttttg atgttcaaat aaactgatac aactatggcg
      781 acaaaagaca ctgaaaatca accaaaagtg aatgtagaaa aattaaaagc acttcaatta
      841 gcacttgata agattgaaaa agaccacgga aaaggcacca ttatgaaaat gggcgataaa
      901 acggtagtag aagtaaacac gatttctaca ggttctattg gactaaacgt tgctttaggc
      961 gttggtggtt ttcctcgtgg aagagtaata gaaatttttg gtcctgaatc gtctggtaaa
     1021 acaaccctag ccattcatgc tatagctcag gcacaaaaag ctggtggaat cgctgctatt
     1081 attgatgctg aacatgcatt cgatcgtttc tatgccgaaa aattaggggt tgatatcaat
     1141 aatctactta tttctcaacc agataacgga gaacaagctc tcgaaattgc cgaccaactc
     1201 attcgctcct cagcagtaga tatagtagtt atagactctg ttgccgcctt aacaccaaaa
     1261 gctgaaattg aaggcgatat gggtgattca aaaatgggat tacaagcacg actaatgtcg
     1321 caagcattac gtaaacttac agccaatatt aacagaacaa atacaacgtg tatatttatt
     1381 aaccaattac gcgataaaat tggcattaca tttggcaatc ctgaaacaac tacgggggga
     1441 aatgcactga aattctacgc ttctattcgc ttagatattc gtcgtatagg acaaattaaa
     1501 gatggagacg aagtgattgg caatcaaaca cgcgtaaaaa ttgtaaaaaa taaggtagct
     1561 cctcctttcc gtaaagctga atttgatatc atgtttggtg aaggaatttc tattgcagga
     1621 gaaattattg atttaggagt tgaacaaggc attattaaaa aaagcggatc gtggtttagc
     1681 tacggcgaca ccaaactagg acaaggtaga gatgctgcaa aaaaagtaat gatagacaat
     1741 ccagaattag ctgccgaatt ggaacaaaaa ataatggaag ctttagcaaa ataaagttcc
     1801 atatcaacac acatttacgc aaagaacaca catgaaaaaa gaatcggaaa tgatatatgg
     1861 catgcatgcc attatcgaag ccattgatgc cggcaaggaa attgataaaa ttatcatgaa
     1921 aaatgatatg caaggacgct tgtctcacga cttgtttaag gcaattgagg gtagaaatat
     1981 acccattatg cgtgttcctg tcgaacgctt agacagaatt accaagaaaa accatcaagg
     2041 agtaattgct tttacaatac cggttgccta tcaacatatc gaagatatta ttcctaccct
     2101 ctatgaagat ggaaaagcac catttattat agtacttgac ggtataaccg atgtacgtaa
     2161 ttttggagcc atagcacgta cctgcgaatg tgcaggtgta catgccattg ttattcccga
     2221 aaaaaatagt gcttctatca atgccgatgc cataaaaact tcggctggag cattactcaa
     2281 aatacctgtt tgtcgagaaa aaaacttgac caatgccata aaattcctta aaaactctgg
     2341 tctaaagatt gtggctacat cagataaagc tgatgataat tatacggata tatcatacac
     2401 agatgcactt gctattgtaa tgggggctga agatataggt atttctatcg aaaaccaacg
     2461 tttgtgcgat actatagtta aaatcccgat agtgggcact atttcgtctt taaatgtatc
     2521 tgtagccact ggtattatgg cgtatgaagt actgaaacaa cgcttgtaac aaaatgaatg
     2581 tattattcga agataaccat ataattgcta tcaacaaatc atgttcagat attgtacaag
     2641 gcgataaaac gggtgatact cctttggttg aaaaggtgaa aatttacctc aaagaaaaat
     2701 acaataaacc tggcaatgta ttttgcggtg ttactcatcg actcgatcgt cctgtgagtg
     2761 gtattgtgct atttgctaaa acgagcaagg ctcttacccg attaaacgaa atgttacgcg
     2821 aacgcgaaat aaaaaaaacc tactgggcta ttacaaaaaa ccgaccagct aaaccagaaa
     2881 tgctattaga gcactacctg cttcgcaacg aaaagcaaaa taaatcgtat gcctacgata
     2941 cgcctaaagg cgatgcaaaa aaagcaactc ttacaattaa agaattggct accagcacca
     3001 attatacatt acttgaagta gatttacaaa caggcagaca tcaccaaatt cgttgtcaat
     3061 tagcacatat cggttgccca ataaaagggg acttaaaata tggctttgca cgctcaaatc
     3121 ctgatggagg tattagccta cacgcccgac aaataaattt tatacatcca gtaaccaaag
     3181 aaccaattga gattattgcc ccaacaccag aagataatct ttggaaggct tttgaagaca
     3241 tgataagaaa tgcataaaac aattctttcg ggcatagtac atgaaattcc tgaagatata
     3301 gcaaaaatat tgcttgaaaa tgatgctatt cttgacaagt ggaatgcact tacacctata
     3361 caacgaaatg aatggatttg ttgggttacc attgtaaaaa aagagcaaac aagagcagaa
     3421 catattatac gtatgatgga tgaattaaaa gcagggaaag gtaaaccgtg ttgttggcca
     3481 ggatgtccac acagacgttc atctgctcaa aaatggttta ctaacaaatc tatgtaatca
     3541 gaaaacaaaa acgaggttgt aacatataat tacaacctcg tttttttata aagtaaaaaa
     3601 ctatcttagt cttctaacaa aatctccata atagtaacag cagcttttgc aaccgatgtt
     3661 ccaggaccaa agatggcagc aacacctgct tgatatagat aatcataatc ttgtgcagga
     3721 attaccccac ccgctattac aatgatatct tcacgtccca aggcttttag ttcagcaata
     3781 acttgtggta ctaaggtttt gtgacctgca gctaatgatg aaacgcccat tatgtgtacg
     3841 tcattttcaa cagcctgttt ggctgcttcg gctggcgttt ggaacaaagg tcccatatcc
     3901 acatcaaagc cacaatcggc ataccctgtg gctacaactt ttgcaccgcg gtcgtgtccg
     3961 tcttgaccca ttttggcaat cataatacgt ggctgacgac cttctttttc agcaaatttt
     4021 gctgttaatt cgcacgcttt aataaagtct gcgtcatttt ttgttcctgc actatacact
     4081 cctgttatgg ttctaatggt tgctttatat cttcctacta tcacttcgca tgcatctgag
     4141 atttcgccca atgttgcacg cactctagct gcttctacag ctaactccaa caagtttcct
     4201 tcgcctgttt ctacacattt ggtaatggca gctaaggcat tttttacagc ggcattatct
     4261 ctggcttgtt tcaattgagt taaacgctca atttgttgta tgcgtacggc tgtattatca
     4321 acttctaaaa tatccaaagg atcttctttt tctaaacggt atttgttggt tccaacaatg
     4381 gtttgctgac ctgagtcgat acgagcttgg gtacgagcag cagcctcttc gatacgcatt
     4441 tttggaatac ctgtttcgat agcagcagcc ataccaccca atttctcaac ttcttcaatt
     4501 aaagcccacg ctttttcagc caattcattc gttaaagact ctacgtaata cgaacctgcc
     4561 caagggtcaa cttcgcgaca aatatccgtt tcttgttgaa tataaatttg tgtgttacga
     4621 gcaatacgag ccgaaaaatc tgttggtaaa gcaatagcct cgtcaagtgc attggtgtgc
     4681 aatgattgtg tatgtcccaa ggcagccgcc attgcctcaa tacacgtacg accaacgtta
     4741 ttaaatggat cttgctctgt tagcgaccat ccagaagttt gcgagtgggt acgcaaagcc
     4801 aaagattttg ggtttttagg attaaattgt tttacaattt ttgcccatag caaacgagct
     4861 gcacgcattt tagcaatttc cataaagtga ttcatgccta ttgcccagaa aaatgataaa
     4921 cgaggagcaa acgaatcgat atccatacct gcatttacac ccgcacgtaa gtactccaat
     4981 ccatctgcca aggtatatgc taactcaata tcagcagtag caccagcttc ttgcatgtgg
     5041 tatcccgaaa tagaaattga gttgaattta ggcatatttt ttgacgtata ttcaaaaata
     5101 tcagcaataa ttttcatcga aaatttaggt ggataaatat acgtattacg caccataaat
     5161 tctttcaaaa tatcattttg gatagtacct gacaattctt caagtttagc accttgctct
     5221 aatcctgcat tgatataaaa agctaacaca ggcaatacag caccattcat cgtcattgat
     5281 acagacattt tattcaatgg aataccatca aacaacactt tcatatcttc gattgaacag
     5341 atagatacac cagctttacc cacatcacct actaccctct cgtggtcggc atcgtatcca
     5401 cggtgagtag ctaagtcgaa agcaacagac aatccttttt gaccagcagc taaatttcta
     5461 cggtaaaacg catttgattc ttctgcagta gaaaatcctg catactgacg aatagtccaa
     5521 ggttgcatag catacattac actataaggt ccacgtaaat aaggaggtaa acccgcagca
     5581 tattgtaaat gttgcatacc atttaggtct tgttttgtgt aaaacggttt cacaggaatt
     5641 tgctcaggtg ttacccaagt actttccgct tgtgtaccaa cgcctgatga aacagcgatg
     5701 tttgtaatgt caatactttt aaaatcaggt ttcatgttca ttttttttaa tttatgagcc
     5761 attcaggaat gtactcaatt ttattattca tttttattat ttctcaatta tttatgcaat
     5821 tggaattagg ataaataccg ctacatccca aacagcatgc gacacaatga gtgttacaat
     5881 atttttgtgg tatttgtaca atatgcccca aaacaatcca cacaccatgg ctgccataat
     5941 taacataaaa ttaaacgacc agatatgtac taaggtataa attaacacag tgattaatac
     6001 agcaaaccaa tcgccatatt ttgcaccaag ttgtttttgc acatatccac gccaaaatat
     6061 ttcttcagca ggaccaatca gcacagtaag taatacgcct actaatacca aattttgtcc
     6121 ttctttcatt ccataaatgc cttgtatttg agtgccagca aaatcaaacc attgggtaga
     6181 taaataatta cctactacaa acaccccata caaaacagca gcagacacca gtcctattac
     6241 aagatcttta acattgaaag agaattgttt gctaaaattt ttatccaaaa aagcactcat
     6301 agcccacaaa attgcagctg aacaagccat tgctatccaa aaattaatat ggatagatgt
     6361 ccatggcgaa aacataataa accacagaac agcagcaagc aatgacgcta ataatacttt
     6421 tttcatatcc aatatccatt aaaaattcca agagcataca tcaatgtaaa tgccatcacc
     6481 aattgagctg acatagcatc taaatctttg ataatctcag gtttatcaat cttagcttgt
     6541 tttattaaat tgatattctt ttttgcaagg ggcaaggtta ctatt
//
LOCUS       DAOSMY010000040         6562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_11829_length_6562_cov_3.782849, whole genome shotgun sequence.
ACCESSION   DAOSMY010000040 DAOSMY010000000
VERSION     DAOSMY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11829_length_6562_cov_3.782849"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLD76_01690"
     CDS             <1..765
                     /locus_tag="PLD76_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU26751.1"
                     /translation="SKLLGAEVVQYDLCEDKGWEPDFEALQKMDLSNVKLMWTNYPNM
                     PTGANASISLYQKIVDFAKKNNIVVVNDNPYSFILNNKPLSILQIEGAKECCIEFNSL
                     SKSHNMPGWRIGMIAANPTFIEWIVRVKSNIDSGTFRAIQLAAVEALSNTNDWHKEMN
                     IDVYQSRRDIAGKIMNALNCSYDDTQVGMFLWGKVPDNALSAEEITEKVLHEAKVFIT
                     PGFIFGSNGERYIRISLCATQAMLEEALNRIQLIIK"
     gene            779..1846
                     /locus_tag="PLD76_01695"
     CDS             779..1846
                     /locus_tag="PLD76_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOU26752.1"
                     /translation="MNDLQSILLPGIESKRPIVIAGPCSAETEEQVMDTAKQLAKNGI
                     KIYRAGIWKPRTKPGGFEGVGEIGLPWLQKVKEETGMYVSTEVATAKHVEAALKAGID
                     ILWVGARTSANPFAVQEIADSLKGVDIPVLIKNPVNPDLDLWIGAIERIYNAGIRKLG
                     AIHRGFSSYDKKIYRNLPQWHIPIELHRRLPNIPIICDPSHIGGRRELIASISQQAMD
                     LSFDGLIIESHICPDKAWSDASQQITPDILQFIINKLVIREGVQTTESLADLRLQIDN
                     IDNDLLEMLSKRMRISREIGTFKKEHNMPVLQANRYDEIMQKRVILGESMGMSPEFVT
                     KILEAIHEESVRQQIDVINSK"
     gene            1867..2640
                     /locus_tag="PLD76_01700"
     CDS             1867..2640
                     /locus_tag="PLD76_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOU26753.1"
                     /translation="MKILILGAGKMGTFFCDVLCTQHEVAIFDTNPKNLRFVFNTYRL
                     TQYDEIAEFAPELVINAVTLKHTIDAFQAVIAYLPSTCILSDIASVKTGLQEFYASTG
                     MRYVSTHPMFGPTFANLEDLSSQSAIIISESDHMGKAFFKDLYQSLKLQIFEYTFLEH
                     DETIAYSLSIPFASTIVFAAVMKHQDAPGTTFKRHLAIARGLFAEDDYLLSEILFNPF
                     TPSQVEKIRGELKFLLEIIEQKDSKAMHSYLQKLRENIQ"
     gene            2721..3017
                     /locus_tag="PLD76_01705"
     CDS             2721..3017
                     /locus_tag="PLD76_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26754.1"
                     /translation="MKKLLLYILLSFAVVGVYATTEKKPVNTVQETQQEVVSVVGNKV
                     YITAKAGEEINIFALTGQNVYRTLSEGGVLVVEDLPTNQILVVRIGAKAYKVKL"
     gene            complement(3078..4070)
                     /locus_tag="PLD76_01710"
     CDS             complement(3078..4070)
                     /locus_tag="PLD76_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU26755.1"
                     /translation="MKEKIPVTIVTGFLGAGKTSLLNMLIKQHPFTKFAIIENEFGEL
                     SVDGGLLATAKENIFELSNGCICCSLQSEFSTVIDRLLSSPYPFNHLLIETTGIANPA
                     TVIRPFLEGDKFPFLFELNAVVCLIDANNYKNKSNNFHEIHRQIAMSDTLIINKTDLI
                     TQEELNSLTKILHTKNPMATIFPNSYNSIDTRKILQAYSYREQQIASSIFSYTPNHTE
                     HKNDSQLITQPTTLSHTIESVAFEYETAFDVDTFSSWIRMFVTLNQQTIFRIKGILCF
                     ANLPGKFILQVVGDIVTFESLPDDTEKNTCSKLVFIGTSIPKTDIEISLRNMLA"
     gene            4213..5160
                     /gene="cysK"
                     /locus_tag="PLD76_01715"
     CDS             4213..5160
                     /gene="cysK"
                     /locus_tag="PLD76_01715"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOU26756.1"
                     /translation="MARIAKNLTELIGNTPLVQLSNYAEKRNLQANVIVKLELFNPAG
                     CVKERIALAMIEDAEADGKLKKGAEIIEPTSGNTGIGLAMVAAVKGYKLTLTMPETMS
                     IERRNLLKALGANLVLTPGALGMKGAIEKAAELHQANPFSYMPQQFDNPSNPSVHQRT
                     TGEEIWRDTNGKVDVFVGGVGTGGTISGVGAALKAHNPAIQIIAVEPADSPVISGGKP
                     GPHKIQGIGAGFIPKNYNPQVVDEVIQVSTDNAILTARQLAREEGMLVGISCGAAAYV
                     ATQVALRPENKGKNIVVLLPDTGERYLSTLLYAFEEYPL"
     gene            5282..>6562
                     /locus_tag="PLD76_01720"
     CDS             5282..>6562
                     /locus_tag="PLD76_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOU26757.1"
                     /translation="MATQLKFETLQIHAGQEVDATTKSRAVPIYQTSSYVFDDAKDGA
                     DLFGLKKFGNIYTRLMNPTTDVFEKRIAALEGGVAALATSSGQSAQFLALNNILQAGD
                     NFVTTSHLYGGTYNQFRSQFKRLGVEVRFTENDEPATFEKYIDSNTKALYLETIGNPD
                     LNIPDFEAIAAVAKKYDIPLIVDNTFGAGGYLFRPLEHGASVVVESATKWIGGHGTSL
                     GGVIVDGGNFNWGNGKFPAFTEPSDSYHGLVFWDVFGANGPFGNIAFIIRARVEGLRD
                     WGNAISPFNSFLLLQGLETLSLRVERHVENALNLAQWLEKHPAVEYVNYPGLKSSKYH
                     SLANKYLKNGFGGVLSFKLKGDAEKADKLINNLKLISHLANVGDAKSLIIHPAATTHE
                     QLSPSEKLAAGVFPGLLRISVGIEHLEDIKSDIEQ"
BASE COUNT         2249 a         1035 c         1299 g         1979 t
ORIGIN      
        1 agtaaattgt tgggtgcaga ggttgtgcaa tacgatttat gtgaagataa aggctgggaa
       61 cccgattttg aagcattaca aaaaatggac ttatcaaatg taaaactcat gtggacaaat
      121 tatccaaata tgccaacagg agccaatgca agtataagtt tataccagaa aattgtagat
      181 tttgccaaaa agaataatat tgtagtagta aacgataatc cgtatagttt tattttaaac
      241 aacaaaccgc taagtatttt acaaatagaa ggggcaaaag aatgttgtat agaatttaat
      301 tcgttgagca aaagtcataa tatgcctggt tggcgtattg gtatgattgc tgcaaatcca
      361 acatttatcg aatggattgt acgggtgaaa agtaatatag attctggtac atttcgtgct
      421 atacagttag ctgcagtaga agcactttct aatacaaacg attggcataa agaaatgaat
      481 attgatgtat atcagtcgcg tagagatatt gctggaaaaa ttatgaatgc tcttaattgt
      541 agttatgatg atacgcaagt aggaatgttt ttatggggaa aagtgcctga taatgcccta
      601 tcggctgaag aaattacaga aaaagtattg catgaagcca aggtttttat tactcctggg
      661 tttatttttg gaagcaatgg cgaacgatat atacgtattt ccttgtgtgc tacacaagcc
      721 atgctcgaag aagcattaaa tagaattcaa ttaataatca aataaaaaat ataaaaacat
      781 gaacgattta caatcaatat tattacctgg tattgagtcg aaacgaccga tagtaatagc
      841 cggaccttgc agtgccgaaa cagaagagca agtaatggat acagccaaac aattggcgaa
      901 aaatgggata aaaatataca gagcaggaat atggaaacct agaacaaaac ctggaggatt
      961 tgaaggtgta ggagaaattg gtttaccttg gttacaaaaa gtaaaagaag aaacaggtat
     1021 gtatgtatct accgaagtag ctactgccaa acacgtagaa gctgcattga aagctggtat
     1081 tgatatactc tgggtaggtg cacgaacttc tgctaatcct tttgctgtac aagaaattgc
     1141 cgattcattg aaaggagtgg atatacctgt gttgataaaa aaccccgtta atccagattt
     1201 agatttatgg attggtgcta ttgaacgtat ttataatgca ggaattagaa aattaggagc
     1261 tatccacaga ggatttagtt cgtatgataa aaaaatatac cgtaatttac ctcaatggca
     1321 tattccaatc gaactacatc gccgtttgcc taatatacca attatttgcg atccaagcca
     1381 tattggagga agaagagaat tgattgcatc gattagtcaa caagctatgg atttaagttt
     1441 tgatggttta attattgaat cacacatttg ccctgataag gcatggagcg atgcttcgca
     1501 acaaataacg ccagatattt tacaatttat aatcaataaa ttggtaatta gagagggtgt
     1561 tcaaacaacg gaaagtttgg ccgatttacg tttgcaaata gacaatattg ataatgattt
     1621 attagaaatg ttatctaaac gtatgcgtat atcgcgtgag ataggcacat ttaaaaaaga
     1681 gcataacatg cctgtactac aagccaatcg ttacgatgag attatgcaaa aacgtgtaat
     1741 tcttggtgag tcaatgggta tgtcccccga atttgtaact aaaatattgg aagcaataca
     1801 cgaagaatca gtgcgtcaac aaatagatgt gattaactct aagtaattta ataaatccat
     1861 aagtatatga aaattctcat attaggtgca ggtaaaatgg gtacattctt ttgtgatgta
     1921 ttatgtaccc aacacgaagt tgctattttt gatactaatc ctaaaaattt acgttttgta
     1981 tttaatacct accgccttac acaatatgat gaaattgcgg aatttgcccc agaactagtt
     2041 attaatgcgg taacactcaa acataccatt gatgcatttc aagcagtaat tgcttatttg
     2101 ccaagtacgt gtattttgtc ggatatcgcg tctgtaaaaa caggtttgca ggagttttat
     2161 gcatctactg gtatgcgata tgtatccact catccaatgt ttggacccac atttgcaaat
     2221 ttggaagatt tgagttcgca aagtgccatt attatttccg aatccgatca catgggaaaa
     2281 gcatttttta aagacttata tcaaagttta aaattgcaaa tttttgaata tacgttttta
     2341 gaacatgatg aaaccattgc ctattcgttg tctatcccct ttgcatctac cattgtattt
     2401 gctgcagtaa tgaaacatca agatgctcca ggtacaacgt tcaaaagaca cttagccatt
     2461 gcaagaggat tatttgccga agatgattat ttgctttcag aaattttgtt taaccctttt
     2521 acgccttcgc aagtagagaa aattcgtggc gaattgaagt ttttactaga aattattgaa
     2581 caaaaagatt caaaagcaat gcattcatat ttgcaaaaat tgcgagaaaa tattcaataa
     2641 atgaattagt aagtattttt tttataaaaa atttgtatat actcaaaaaa tcatatatat
     2701 ttgcagaaat atactaacct atgaaaaaat tactactcta catattgctc tcttttgcgg
     2761 tggttggggt gtatgctact accgaaaaaa agcccgtaaa tacggtgcaa gaaacgcaac
     2821 aagaagttgt ttcagtagtg ggtaataagg tatatattac agctaaggca ggagaagaaa
     2881 taaacatatt tgctcttact ggtcaaaatg tatatcgtac tctttccgaa ggaggagtgt
     2941 tggtggtaga agatttacct acaaaccaaa ttttagtagt acgtattgga gccaaagctt
     3001 ataaagtgaa attataaaga taaaagattt gttgttagtt ttatgggaga tgcaattttg
     3061 tgtctcctat ttttttgtta agcaagcatg ttgcgtaaac ttatttcgat atccgttttc
     3121 ggtatcgatg tgcctataaa tacaagtttt gagcacgtgt ttttttcggt atcatctggc
     3181 aatgattcaa atgtaacaat atcccccacc acctgtagta taaactttcc aggtaaattt
     3241 gcaaaacaca aaatcccttt tattctaaaa atagtttgtt ggttgagtgt aacaaacatc
     3301 cgaatccaac tactaaatgt atctacgtca aaggctgttt cgtattcaaa tgctaccgat
     3361 tctatagtat gagagagcgt agtaggttgt gtaatgagtt ggctgtcgtt tttatgctca
     3421 gtatggtttg gtgtatacga aaatatagat gaagctatct gttgttctct gtaagaatag
     3481 gcttgaagta tttttcttgt atcgatagag ttatatgaat ttgggaagat agttgccatt
     3541 ggattttttg tatgcaaaat ctttgtcaac gaattaagtt cttcttgtgt aattaaatca
     3601 gttttattga tgatgagggt atccgacatg gctatttgtc gatgaatttc atgaaaattg
     3661 ttgcttttat tcttatagtt gtttgcatca attaaacata ctactgcgtt taattcaaac
     3721 aaaaatggaa atttgtctcc ttccaaaaaa ggtcttatta ccgttgcagg attggctatg
     3781 cctgttgttt cgatgagtag gtggttaaac ggatacggac tagaaagcaa tcgatcaata
     3841 acggtagaaa attctgattg taacgaacaa cagatgcatc cgttgctcaa ttcaaagata
     3901 ttctctttgg cagtagctaa taaacctcca tccacggata gttcgccaaa ttcgttttcg
     3961 ataattgcaa atttggtaaa cgggtgttgt ttgataagca tgtttagcaa acttgttttg
     4021 ccagctccca aaaatccagt aacaatggta acaggtattt tttctttcat acgtataaat
     4081 aagcatgcga aaatacatat ttttgcttga tataccatac ttgtggtatg taaaatccca
     4141 cacaaaagct attgtgtatg catttattac cagttatctt tgcatcagtt aaacattaaa
     4201 aaaatacatg gtatggcacg aatagcaaaa aacctaacag aacttattgg aaatactcca
     4261 ttagtacaat tatcaaatta tgccgagaaa agaaatttac aggctaatgt aatcgtaaaa
     4321 ctcgaattat ttaacccagc aggttgcgta aaagaacgca tcgctttagc catgatagaa
     4381 gatgcagaag ccgatggaaa attaaaaaaa ggagccgaaa ttattgaacc aacgagtgga
     4441 aatactggca taggattagc tatggtagct gctgtaaaag gatacaaact tacgcttacc
     4501 atgcccgaaa ctatgagcat tgaaaggcgt aatttattaa aagcattggg tgcaaatttg
     4561 gtattaactc caggggctct aggaatgaaa ggagctattg aaaaagcggc agaattacat
     4621 caagccaatc cattttcgta tatgccacag caatttgata acccatctaa cccatcagtt
     4681 catcaacgta caactggcga agagatatgg agagatacca atgggaaagt agatgttttt
     4741 gtaggtggtg taggcactgg aggaacaatc agtggtgtag gagctgctct taaagcacat
     4801 aatccagcca tacaaattat tgcagttgaa cctgctgatt ctccagtaat ttcaggtggt
     4861 aaacctggtc cgcacaaaat acaaggcata ggagcaggct ttattccaaa aaattataac
     4921 cctcaagtag ttgatgaggt gatacaagta tccacagaca atgctatttt aacagcacga
     4981 caattggctc gagaagaagg tatgttagta ggtatttcgt gtggagctgc tgcttatgta
     5041 gctactcaag tggctttgcg tccagagaat aaagggaaaa atattgttgt attattacca
     5101 gatactggcg aacgttattt gtctacactt ttatacgcct ttgaagagta tccattataa
     5161 gaaattgaaa acaacatata gtatgagtaa tttatcagaa gcttttcaac aagcattagc
     5221 aaaaatacta aaacagcgag taaaaaatac aaatatagaa aaacaaatac aagataaaac
     5281 gatggcaaca caactaaaat ttgaaacatt acagatacat gcaggtcaag aggtagacgc
     5341 aacaacaaag tcaagagcag tacccatata tcaaactagt tcgtatgtgt ttgacgatgc
     5401 caaagatggg gcagacctat ttggattaaa gaagtttggt aatatttata cccgattaat
     5461 gaatcctact accgatgtgt ttgaaaaacg tatagcagcc ctcgaaggag gagtagcagc
     5521 tttagcaaca tcttcgggtc agtcagctca atttttggct ttaaataata tcttacaagc
     5581 tggcgataat tttgtaacta cttctcattt atatggaggt acgtacaatc agtttaggtc
     5641 tcagtttaaa cgattaggag tagaagtgcg ttttaccgaa aacgacgaac cagctacatt
     5701 tgagaaatac attgacagca ataccaaagc cttatattta gaaacaatag gtaatccaga
     5761 tttgaatatt ccagattttg aggcaattgc agcagtagcg aaaaaatatg atatcccctt
     5821 aatagtagat aatacatttg gagctggtgg ttatttgttt cgcccgctag aacatggagc
     5881 ttccgtagta gtcgaaagtg ctactaaatg gattggcgga catggcactt cgttaggtgg
     5941 tgtaattgtt gatggaggta attttaactg gggaaatggt aagtttcctg cttttacaga
     6001 gccatcagat agttaccatg gattggtatt ttgggatgtg tttggtgcaa acggaccatt
     6061 tggaaacata gcgtttatca tcagagcacg tgtagaaggg cttcgagatt ggggaaatgc
     6121 aattagtcca ttcaactcat ttttgctatt gcaaggacta gaaaccctgt cgttgagagt
     6181 agaaagacac gtagagaacg cgttaaactt agcacaatgg ttagaaaaac atccagcagt
     6241 agaatacgta aattatccag gtctcaaaag tagtaaatac cattccttgg ctaacaaata
     6301 tttaaaaaat ggttttggcg gtgtattgtc gttcaaatta aaaggagatg cagaaaaagc
     6361 agataagtta attaataatt taaaactaat aagccatcta gcaaatgttg gtgatgccaa
     6421 atcattgatt attcatccag ctgccactac ccacgaacaa ctttcacctt ccgaaaagct
     6481 cgccgcaggc gttttcccag gcttgttgcg tatctctgtg ggcatagaac atcttgaaga
     6541 tataaaatca gatatagaac aa
//
LOCUS       DAOSMY010000041         6452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12095_length_6452_cov_3.423792, whole genome shotgun sequence.
ACCESSION   DAOSMY010000041 DAOSMY010000000
VERSION     DAOSMY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12095_length_6452_cov_3.423792"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLD76_01725"
     CDS             <1..756
                     /locus_tag="PLD76_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU26758.1"
                     /translation="RQFMHKYDERGSLAPRDIVARAIDSEMKLSGDDFVYLDVTHKSA
                     EETKAHFPTIYQKCLSLGVDITKDYIPVVPAAHYLCGGIEVDVHSQSSIRNLYAAGEC
                     SRTGLHGANRLASNSLIEAVVYADKAATHALGRIEGVVFNEQVPEWNDEGTSLNEEMV
                     LITQSAKEVGQIMSNYVGIVRSNLRLKRAMDRLEILYKETEDLFTRSVVTKDICELRN
                     IINTAYLVIKMAMQRKESRGLHYTLDYPDKIEL"
     gene            complement(763..1566)
                     /gene="proC"
                     /locus_tag="PLD76_01730"
     CDS             complement(763..1566)
                     /gene="proC"
                     /locus_tag="PLD76_01730"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOU26759.1"
                     /translation="MKKISIIGAGNMGGAIARGLSQGSVFSAQQITVSDISTENLQAI
                     KTFNPLINTTLSNVDGVQHADIVVIAVKPWLTETICNEIKSVLNYDKQVVVSIVAGVS
                     FEQLHTFLKKEGESSPTIFRVIPNTAIDVLQSVSTIASSHANKQQEEEIVRIFNELGK
                     AFLVSSEQQLNAFMSLSSCGIAYAFRYIRAAVEGGVEMGIYPETAKQVVLQTLRGAID
                     LLEATNAHPEAEIDKVTTPAGITIKGLNEMEANGFSNAVIKGLKASYIK"
     gene            complement(1658..2179)
                     /locus_tag="PLD76_01735"
     CDS             complement(1658..2179)
                     /locus_tag="PLD76_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOU26760.1"
                     /translation="MISKKMQDAINAQINAEFWSAYLYLSMSNNFAAKGNPGFANWFE
                     IQFKEEQDHAMIFGKHILARGGKVSLAPIKEVKTEWATPLEAFEETLKHEKVVTGLIN
                     NLYKIAVEENDYATQSMLKWFIDEQVEEEETAQELIDSLKMIANNGFGIYMLDKELST
                     RTYKTPSPLVAKV"
     gene            complement(2229..3350)
                     /locus_tag="PLD76_01740"
     CDS             complement(2229..3350)
                     /locus_tag="PLD76_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU26761.1"
                     /translation="MKLFDVYPLFPIEIVKGKGCKVYDNNGTEYLDLYGGHAVISVGH
                     AHPTYVQAVADQVGKLGFYSNSVINGLQETFAEKLGIACGYPDYSLFLINSGAEATEN
                     ALKLASFHNGKKKVLAFKKAFHGRTSVAVKVTDSPSYVAPINTGLDVTFLALNDIEGV
                     KKELATGDYTAVIIEGIQGIGGIIEPNIDFLQALRLECTQTNTVLILDEIQSGYGRSG
                     KFFAHQYAGIKADIITVAKGIANGFPFAGVLISPMFKAVHGQLGTTFGGNHLGCAAAL
                     AVLHIIEKENLIENAKQIGDYLLHELKQIPQIKEVRGKGLMIGIDFEQAVKEIRNKLL
                     VEQHVFTGVAGTHTIRLLPPLCLTKEDATEFLLKLKQCL"
     gene            complement(3391..4353)
                     /gene="argC"
                     /locus_tag="PLD76_01745"
     CDS             complement(3391..4353)
                     /gene="argC"
                     /locus_tag="PLD76_01745"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOU26762.1"
                     /translation="MIKIGIIGGAGYTAGELLRLLVHHPQAEIVFVNSTSNAGNKLYA
                     VHSGLIGETELEFTNELPLDAIDALFICSAHGDSRKFLDANTIPSKVKIIDLSTDFRD
                     ESNGFVYGLPELNKQRILQANKIANPGCFATVIELALLPLAANGLLTNEVHINAITGS
                     TGAGVKPSSTSHFSWRNNNISVYKAFEHQHLKEITQSITQLQPSFGKAINFIPVRGNF
                     ARGIFASVYTDCSLSLEEAKALYKNYYKDAAFTFVTDTNPDLKEVVNTNKCIVYLEKH
                     GDKLLILSCGDNLLKGASGQAVQNMNLLFGLDEKMGLNLKNIAF"
     gene            complement(4356..5558)
                     /locus_tag="PLD76_01750"
     CDS             complement(4356..5558)
                     /locus_tag="PLD76_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOU26763.1"
                     /translation="MKKEKVLLAFSGGLDTSFCAMYLAKDKGYEVHTAIANTGGFSPE
                     ELKRIEERAYRLGAKTHATLDVTQEYYDKSIKYMVFGNVLRNGTYPISVSSERIFQAI
                     ATIEYAKKIEADYIAHGSTGAGNDQVRFDLTFDVLAGHIKVLTPTRDMVLTREYEINY
                     LKEHGYEADFTKMEYSINAGLWGTSIGGKETLKSEQTLPESAYTKQVVKKEEETITID
                     FEKGEIVGVNNVHYTDKIAAINKVEEVGSAFGIGRDMHIGDTIIGIKGRVGFEAAGAL
                     LIINAHKMLEKHTLTKWQQYWKDQLGNWYGMFLHEAQYLEPVMRDIEKFLENSQQNVT
                     GKVIIKLRPYSYTLVGVDSPFDLMKTDFGEYGEVNRAWSADDVKGFTKILGNQMKIYY
                     NVQAKNNK"
     gene            complement(5571..6209)
                     /locus_tag="PLD76_01755"
     CDS             complement(5571..6209)
                     /locus_tag="PLD76_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU26764.1"
                     /translation="MESDTIKIMVANESHYTYIDEILSTIEESSKDRGTGIAKRSPEY
                     VKNKMAEGKAIIALDGEVFAGFCYIESWGNKQYVANSGLIVNPKYRGHGVAKKIKKFS
                     FDLSRQRFPNAKLFGLTSGAAVMKINTELGYVPVTFADLTDDEEFWKGCQGCVNFDVL
                     QRTNRRYCICTAMLYDPNDPNRRNREEKENEAKKQNKKSVITDFINKFKKLK"
     gene            complement(6231..>6452)
                     /locus_tag="PLD76_01760"
     CDS             complement(6231..>6452)
                     /locus_tag="PLD76_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOU26765.1"
                     /translation="TREIISIDFSGQLAVIKTRAGYANGIASDIDQQLAELILGTIAG
                     DDTILLIPKEGITRNTITTAIRSFMTVIS"
BASE COUNT         1834 a         1257 c         1150 g         2211 t
ORIGIN      
        1 cgtcagttta tgcataaata cgatgaacga ggctcgctag ctccacgtga tatagtggca
       61 cgggctatcg actctgaaat gaaactttcg ggagatgatt ttgtctattt agatgttaca
      121 cataaatcgg cagaagagac aaaagcacat ttccctacaa tttatcaaaa atgtttgtcg
      181 cttggtgtgg atattaccaa agattatata cccgtagtgc ccgcagctca ttatttgtgt
      241 ggtggtatag aggtagatgt acattcgcag tcaagtattc gcaatttgta cgcagccgga
      301 gaatgttcgc gaactggatt gcacggtgct aaccgtttgg catctaattc tttaattgaa
      361 gcggtagtgt atgccgacaa agctgctacg catgcgttag gtaggattga aggtgttgtt
      421 tttaatgagc aagtgccaga atggaatgat gaaggtacct cgctcaacga agaaatggtg
      481 ttgataacac aaagtgccaa agaggtgggg caaattatgt ctaattatgt gggtattgta
      541 cggtctaatt tgcgtttaaa acgagccatg gatcgattag agattttgta caaagaaacc
      601 gaagatttgt ttacccgttc agtggtaacc aaagatattt gcgaacttcg taatatcatc
      661 aataccgcct atttagtaat aaaaatggca atgcaacgca aggaaagtag ggggttgcat
      721 tacacactcg attaccccga taaaattgaa ttataaggca gtttatttta tatacgatgc
      781 ttttaagcct ttgataacag cgtttgagaa tccgttggct tccatttcgt ttaagccttt
      841 gatggtaata ccagcaggtg tggttacttt gtcaatttcg gcttcgggat gtgcattggt
      901 agcttcgagt aggtcaattg ctccgcgcaa tgtttgtagc actacttgtt tggctgtttc
      961 tggataaatg cccatttcta caccaccttc tacagcggca cgtatatacc gaaaggcata
     1021 cgcaatacca caacttgaaa gcgacataaa tgcgttgagt tgttgttcgg aactaactaa
     1081 aaaagctttc ccaagttcat tgaatatacg tacaatttct tcttcttgtt gtttgtttgc
     1141 atgcgatgag gctatggttg aaacgctttg caatacatca atagccgtat ttggaataac
     1201 cctaaatatg gttggactgg actctccctc tttctttaaa aatgtatgta actgctcaaa
     1261 agaaacacca gcaacaattg acacaacaac ttgtttgtca tagttcaata cagatttaat
     1321 ttcgttacaa attgtttcgg taagccaagg tttcacagct atcacaacaa tatcagcatg
     1381 ttgcacccca tctacattac taagagtcgt attaataaga ggattgaagg ttttaattgc
     1441 ctgtaagttt tcggttgaaa tatcgcttac agtaatctgt tgagctgaaa agacagatcc
     1501 ttgagataat cctctagcaa tagctccacc catattgcct gctccaataa tgctgatttt
     1561 tttcataaga agtatgttat taattccaat ccagtaaatg atagttaaaa ataaagtcga
     1621 aaaagaatac tccattttcg accttacgaa ttttctttta tacttttgct actaaaggac
     1681 ttggtgtttt gtatgtgcga gtgcttaatt ctttatcaag catataaata ccaaaaccat
     1741 tgttggcaat catttttagg ctatcgatta gctcttgagc tgtttcttct tcttctactt
     1801 gctcgtcgat aaaccatttt agcatacttt gggtagcgta atcattttct tctacagcaa
     1861 ttttgtatag attattgatt aagccagtaa ccactttttc gtgtttaagg gtttcttcaa
     1921 aagcttccaa cggagttgcc cattcggttt tcacttcttt gattggagcc aaagatactt
     1981 ttcctccacg tgccaaaata tgtttgccaa aaatcattgc atggtcttgt tcttctttaa
     2041 actgtatttc gaaccagttt gcaaaaccag gatttccttt tgcagcaaaa ttattggaca
     2101 tagataaata aaggtatgca gaccaaaatt ctgcatttat ttgtgcgtta atcgcatctt
     2161 gcattttttt actaatcata acggttcatt ttttatgggt tatacattac cacaaatata
     2221 caacattttt acaaacattg ttttagtttt aacaaaaatt cagtagcgtc ctcttttgtt
     2281 aaacaaagtg gaggcaataa acggatagta tgtgtgcccg ctacgcctgt aaatacgtgc
     2341 tgttctacta ataatttgtt tcgaatttcc tttacagctt gttcaaaatc tataccaatc
     2401 atcaatcctt tgcctctaac ttcttttatt tgagggattt gttttagttc gtgtagtagg
     2461 taatcgccaa tttgttttgc attttcgata agattctctt tttcgataat gtgcaacaca
     2521 gccaatgcag ccgcacaacc taaatgattg cctccaaaag ttgtgcctaa ttgtccgtga
     2581 acggctttaa acattggact aataagcaca ccagcaaaag gaaatccatt tgcaatacct
     2641 ttggcaaccg taatgatatc tgccttgatg ccagcatatt gatgagcaaa aaacttgcca
     2701 ctacgaccgt atcctgattg aatttcatcc aaaataagta ccgtatttgt ttgtgtacat
     2761 tccaaacgta atgcttgcaa aaaatcaata tttggctcaa taataccgcc aatgccttgt
     2821 atgccttcga tgataacagc cgtataatcg cctgttgcaa gttctttttt tacaccttca
     2881 atgtcgttaa gagctaaaaa tgttacgtct aaaccagtgt tgataggtgc cacgtagctt
     2941 gggctatccg ttacttttac agctacagag gtacgtccat gaaaagcttt cttgaatgcc
     3001 aatactttct tttttccatt atgaaaggaa gctaatttca gtgcattttc agtagcttct
     3061 gcacccgagt tgattaaaaa tagcgaataa tcggggtatc cacacgcaat gcctaatttc
     3121 tcggcaaatg tttcttgcaa gccattgatt accgaattgg agtaaaaccc taattttcct
     3181 acttgatctg ctactgcttg cacatacgta gggtgtgcat gcccaacaga aataacagca
     3241 tgtccaccat ataagtcgag gtattcagta ccattgttat catatacttt gcatcctttc
     3301 ccttttacaa tttcaatagg gaatagcgga tatacatcaa ataatttcat tttttcattt
     3361 tttaagataa cgaacaatgt cgttttgtaa ctaaaatgca atattcttca aatttaagcc
     3421 cattttttcg tcaagcccaa atagtagatt catgttttga actgcttgtc cagaagcccc
     3481 tttcaataaa ttatcaccac acgataaaat aagtaattta tcgccatgtt tttcaagata
     3541 tacgatacac ttgtttgtgt ttactacttc ttttaaatca ggatttgtat ctgtaacaaa
     3601 cgtaaacgca gcatctttgt aatagttttt gtacaaggct ttcgcttctt ctagcgatag
     3661 gctgcaatcg gtgtatacag aagcaaaaat accacgagca aaattacccc ttacaggaat
     3721 aaaattaatt gctttgccaa aagagggttg caattgtgta atagattgcg taatttcttt
     3781 caggtgttgg tgttcaaatg ctttgtaaac agaaatatta ttgtttctcc aactaaaatg
     3841 cgatgtgcta cttggtttta cacctgcccc tgtagagcct gtaatagcat ttatatgcac
     3901 ttcgttggta agcaatccgt tagctgccaa aggtaacaga gccaattcaa tgaccgttgc
     3961 aaagcatcct ggattagcga ttttattcgc ttgcagaata cgttgtttgt ttaattctgg
     4021 tagtccgtat acaaaaccat tgctctcgtc gcgaaaatcg gtagataaat caataatttt
     4081 taccttagaa ggaattgtat tcgcatctaa aaatttacga ctatcaccat gagctgaaca
     4141 gataaacaaa gcatcaatcg catccagcgg tagttcgttg gtaaattcca attctgtttc
     4201 gccaatcaat ccgctatgaa ctgcatataa tttatttcca gcattactgg tgctatttac
     4261 aaatactatt tctgcttgtg ggtgatgtac caacaaacgg agtaattcgc ccgctgtata
     4321 accagcacct cctataattc caatttttat catctttatt tgttgttttt agcctgaacg
     4381 ttataatata ttttcatctg gttgccgagt attttggtaa acccttttac atcgtcagcc
     4441 gaccaagctc tgtttacttc gccatattcg ccaaaatcgg ttttcatcaa atcaaacggg
     4501 ctatctacac ctactaaggt atacgaatag ggtcgcagtt tgatgatgac ttttccggta
     4561 acgttttgtt gcgaattttc taagaatttt tcaatatcac gcattacggg ttctagatac
     4621 tgagcttcgt gtaaaaacat tccgtaccaa tttcctaatt ggtctttcca atattgttgc
     4681 catttggtaa gagtgtgttt ttcgagcatt ttgtgagcat taataataag caatgcccct
     4741 gctgcttcga aaccaacacg cccttttata ccaataatag tgtcgccaat atgcatatct
     4801 ctaccaattc cgaaagctga acctacttct tctaccttat tgatagctgc aattttgtcg
     4861 gtatagtgta cattatttac gcctacaatc tctccttttt caaagtcaat tgtaatagtt
     4921 tcttcttctt tttttactac ttgcttggta tatgccgatt ctggcaatgt ttgttccgat
     4981 tttaatgttt ctttgccacc aatagatgtt ccccacaatc ctgcgttgat ggaatattcc
     5041 attttggtaa aatcagcttc gtaaccatgt tcttttaggt aattaatttc gtattcgcgt
     5101 gttaatacca tatcacgtgt tggcgttaat accttaatat gacctgctaa tacgtcgaac
     5161 gttaaatcaa aacgcacttg gtcgtttccc gcacctgtag atccatgagc gatgtaatct
     5221 gcctcaattt tttttgcata ttcgatggtg gcaattgcct gaaaaatacg ctctgaactt
     5281 acagagattg ggtatgtgcc attgcgtaac acattgccaa acaccatgta tttaatactt
     5341 ttatcgtagt attcttgcgt tacatcaagg gttgcatgtg ttttggctcc tagtctgtag
     5401 gctctttctt caatacgttt taactcttcg gggctaaatc cacctgtatt agcaattgca
     5461 gtatgcactt cgtaaccttt atctttggct aaatacatgg cacaaaaaga agtgtcgagt
     5521 ccaccactaa atgctaataa tactttttct tttttcatga tacgggtaat ttattttaat
     5581 tttttaaatt tattgataaa gtcggtaata acgctttttt tgttttgttt ttttgcttcg
     5641 ttttcttttt cttccctgtt tctacgatta ggatcgtttg ggtcgtacaa catagctgta
     5701 caaatacaat agcgtctgtt ggtgcgttgt aatacgtcaa agttaacaca tccttggcat
     5761 cctttccaaa attcttcatc atcggtaagg tcggcaaagg taacgggtac gtatcctaat
     5821 tctgtattga ttttcattac agctgctccc gacgttaacc caaataactt ggcgtttgga
     5881 aaacgttggc gagataaatc aaaggagaat tttttaatct tttttgcaac gccgtgccct
     5941 ctatatttag ggtttacgat taatcccgag tttgctacat attgcttgtt tccccacgat
     6001 tcgatgtaac aaaaaccagc aaatacctcg ccatcaagtg cgataattgc tttgccttct
     6061 gccatcttat tttttacata ttcgggcgat cgtttggcaa tacctgtgcc acggtctttt
     6121 gacgactctt caatggtaga aagaatttcg tcaatgtatg tatagtggct ttcgttagca
     6181 accattattt ttatagtgtc agattccata caaataagtt atataattag ttatgaaata
     6241 actgtcataa atgagcgtat tgctgtagtg atggtattgc gagtaatacc ttctttaggg
     6301 attaataata ttgtatcatc tccagcaatg gttccaagta ttaattcagc aagttgttga
     6361 tctatatcag atgcaatacc attggcatag cctgctctcg ttttaattac tgctaattgt
     6421 ccagaaaaat caatagagat aatctctcgc gt
//
LOCUS       DAOSMY010000042         6419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12187_length_6419_cov_3.259114, whole genome shotgun sequence.
ACCESSION   DAOSMY010000042 DAOSMY010000000
VERSION     DAOSMY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12187_length_6419_cov_3.259114"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLD76_01765"
     CDS             complement(<1..377)
                     /locus_tag="PLD76_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOU26766.1"
                     /translation="MKQFLKFTFASCLGFLLSFFTICIIVAIVIVATVTGFSSKQITV
                     APNSVLKIEFNGVLKEQARDMSFFDAWSSTNITELGIHDIIKSIQHAKTDENIVGIWL
                     QMDLFSAGNASISEIRDALVDFK"
     gene            600..1619
                     /locus_tag="PLD76_01770"
     CDS             600..1619
                     /locus_tag="PLD76_01770"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOU26767.1"
                     /translation="MEEINWSNLPFSYMPTDCNIRCSYQNGEWGELRKHTDVYLNMHM
                     AATCLHYGQECFEGLKAFRGKDGKIRVFRIDENALRMQSSCEGILMAKFPVDKFKEAV
                     KLVVKENERFVPPYESGASLYIRPLIIGTSAQVGVNAAKEYEFILFVTPVGPYFKGGF
                     QTTPFVILRQFDRTAPLGTGMYKVGGNYAASLVAVDKAHKMGYSNVFYLDAKEKKYID
                     ECGAANFFGIKNNTYITPKSTSILPSITNKSLMVLAQELGLKVEQRPVPEEELATFEE
                     AGACGTAAVISPIQRIDDVEEGKSYVFSADGKPGKVCEQLYTKLRAIQYGDEPDKYGW
                     ITVIE"
     gene            1661..2074
                     /gene="aroQ"
                     /locus_tag="PLD76_01775"
     CDS             1661..2074
                     /gene="aroQ"
                     /locus_tag="PLD76_01775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU26768.1"
                     /translation="MQLVIINGPNLNLLGKREPQVYGNQTFEEMLAEIRKAFPSIDIL
                     YFQSNVEGEIINKIHEVGFTADGIIINAGAYTHTSIAIADAVKSIKTPVVEVHISNVF
                     ARESYRHESFLTPVCKGCISGFGIKSYYLAIQSFF"
     gene            2151..4592
                     /locus_tag="PLD76_01780"
     CDS             2151..4592
                     /locus_tag="PLD76_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU26769.1"
                     /translation="MKYKYVLSLLWLFFVVFIIRAQDGKIIGQVFDNTSKKPLDFANV
                     ALFTNQSKEPLQAILSDSEGKFILQNLPYGSFTLKVSFVGYESFETSVLVSKEKPTAR
                     VNKVVLKENAQQISEVQVVGQRSGVSFEIDKKVFNVDETALAQGSSTTDVLKNIPSVA
                     VDLEGNVSLRNNSSVEIWINGKPSGLTDENRGQVLEQMPAETVEKIEVITNPSARFNP
                     EGSAGIINIVLKEKNRGSYLASINSNIGYDESGAINGSFGGNYMYNGPKFDVQLNGSI
                     RGGERKNASYNNRATLVAEDTISHLNQRSLSESDMFMGFMRGGVTYHIDKKNDIGLSG
                     FGMLGDFSGNSTINYHSLDANRDTLETRDRLADNANNRMHYNVSLDYKHFFDKTNHEL
                     SSSIYYVRGNHNSFNAYNTSRFGNDGVYINDSKTYQEQQATRNMYNITWQADYFNKFT
                     KDSKLESGLSFNYRNSLSEDRMYDSIFTTNSFVEDVDKYNRFTYVENIFSAYTTYSQK
                     IQTFSFQLGLRAEQTMTEAKQHYRSYFSLFPSVFLSQSLPQNNELQINYTRRINRPRG
                     WQLNDFVNRTDELNLSYGNPSLRPEFTNSFEFNYIKNWEQGHTFSTSLYYRATSDVIQ
                     RVSKLQNEIMETTYENITTSESAGAELILKNRLFANKLDLTSTLNAYYYALAANESYG
                     ISSVENFTWNARMNANIIIQRGFTTQLTAYYRAPQVKVQGRDEQVYGIDLGARKTFFK
                     GALNVGVSVRDLLDSQRTTNITWGENFYQESASLYSGRSYRLTLTYNFGNMKNGGKEQ
                     RNKNNNGFSEENLEF"
     gene            4641..5411
                     /locus_tag="PLD76_01785"
     CDS             4641..5411
                     /locus_tag="PLD76_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26770.1"
                     /translation="MKKLILLALGFLVATCMGVAQVGVPVVKSTNAPNKDKVNVSTIT
                     FNYPAKDIETAVLEKLKKEGLTPRKSKSKFVVFAGVEYFPLWDNKFDFYLRISGSNNA
                     GTVELLMTQGYNNYINPTTPEVQERVNKWLLGLEKTVSDYIYERAMAEHLKEKKLIER
                     TLSSLQTQQDRLTNQYDNLNKQQEAFEKTKVDVSKADPNTLNTKDIAKQQKQQEKIVK
                     ERNKLEYSLYDVRSRIAASQKDLDNRNKAIDELKQQKR"
     gene            5411..5893
                     /locus_tag="PLD76_01790"
     CDS             5411..5893
                     /locus_tag="PLD76_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26771.1"
                     /translation="MARLLLFLIPLLLVSCVAPADYNDSLIREQRKVLSAINDLNTAI
                     KQANKEETNSELAEAVNQVEKSILKVQQKDAFRGETQLREEMEELLVFYKFVLNEYYP
                     LLIEIQHDEGLTLTERKQVSDSIYDTIREQEIQRTTAYAEAQKLFADKYNISIIHTQK
                     "
     gene            5902..>6419
                     /locus_tag="PLD76_01795"
     CDS             5902..>6419
                     /locus_tag="PLD76_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOU26772.1"
                     /translation="MEETLLQLFLRVIGEQPTSVVALPIAGSNRKYLRMCTKKQSFIG
                     VVGTVLEENKAFITIANQFVSKGLPVPRVVGVSDDEMCYLVEDLGDVSLFDSIKKGRE
                     TGEFSAQDIELLKKTIAYLPSLQIEGANGFDFSICFPQAAFNYRTVMWDLNYFKYCFL
                     KPAGIECNEEWL"
BASE COUNT         2202 a         1011 c         1254 g         1952 t
ORIGIN      
        1 tttttaaaat cgactaatgc atctctaatt tcgcttatag aggcatttcc agcactaaat
       61 aaatccattt gcaaccaaat tccaacaata ttttcgtctg ttttggcatg ctgaatgctc
      121 tttattatat cgtgaatacc aagttcggtt atatttgtgg atgaccacgc atcaaaaaag
      181 gacatatctc ttgcttgctc tttcaaaacg ccgttaaatt caatttttaa tacagaatta
      241 ggtgcaaccg ttatttgttt agatgaaaat ccagtaacag tagcaacaat aactattgca
      301 actataatgc atattgtaaa aaaagaaagt aagaagccca agcaagaagc aaaagtaaat
      361 ttcagaaatt gtttcatctt gaatataaaa tatgtacaaa attatacgta aagtaaatca
      421 taaactacaa agatagcgat ttaaagatta aaataaaata gttttgtgtc aaattaaaat
      481 gtagtaaaaa aaacttctat aaaaaccaga catacaatac ttgtatttgc taaaaaaaca
      541 ttacttttgc acttatatat ttattacaca tagctataaa catttttaga atttcgcaga
      601 tggaagaaat taactggtcg aatttgccgt ttagttatat gccaacggat tgtaatattc
      661 ggtgttcata tcaaaacgga gagtggggag aattacgaaa acatacagat gtgtatttga
      721 atatgcacat ggctgctact tgtttgcatt acgggcaaga atgtttcgaa ggactgaaag
      781 cttttagagg aaaagatggt aagattcgtg tgtttaggat tgatgaaaat gcacttcgta
      841 tgcaatcgtc gtgtgaaggt atactgatgg caaaatttcc agttgacaaa ttcaaagagg
      901 cggttaaatt ggttgtaaaa gaaaatgaac gctttgtgcc accttacgaa agtggagcct
      961 cgttatacat acgtccgtta attataggta ccagtgctca ggttggtgtg aatgcagcaa
     1021 aggaatatga gtttattttg tttgtaacgc ctgttggtcc gtattttaag ggaggttttc
     1081 aaactactcc gtttgtgata ttacggcagt ttgatcgtac tgctccttta ggaacaggta
     1141 tgtacaaagt gggtggtaat tatgcagcaa gcttggttgc tgtagataaa gctcataaga
     1201 tggggtattc aaatgtattt tatttagatg caaaagaaaa aaaatacatt gatgaatgtg
     1261 gtgcagcaaa cttttttggg attaaaaaca atacgtacat aacaccaaaa tcaacgtcaa
     1321 ttttaccatc aataaccaac aaaagtttaa tggttttggc acaagaattg ggtttaaaag
     1381 tagagcaacg tccagtccct gaagaagaat tggctacttt tgaagaagct ggagcatgcg
     1441 gaactgcagc tgtaataagt cctattcagc gaattgatga tgtagaggaa ggaaagtcat
     1501 atgtgttttc tgcagatggt aaacctggga aagtatgcga acaattatat actaaattac
     1561 gtgctattca atacggagat gagccagata aatatggttg gataacagtt attgagtaaa
     1621 aaaacgaaca accaactgtc tttacattga aacttacgct atgcaattag ttattataaa
     1681 tggtcctaat ttaaatttgc ttggcaaaag agagccgcaa gtttatggaa atcaaacatt
     1741 tgaggaaatg cttgcggaaa tccgaaaagc atttccatca attgatattc tgtattttca
     1801 atctaacgta gaaggcgaaa ttattaataa aattcatgaa gtagggttta ctgctgatgg
     1861 tattattatc aatgcaggag cctatacaca tacttctatt gctattgccg atgctgtaaa
     1921 atctattaaa actcctgtcg tagaagtaca tatatctaat gtttttgctc gcgaatcata
     1981 ccgacacgag tcttttttaa ctcccgtttg caaaggctgt atttctggat ttggaattaa
     2041 atcctattat ttagccatac agagtttttt ttgacataaa ataacatttc cgctttaaac
     2101 ctttttctct tttttccgtc atagaggcac aacacctaaa cgtaacgaat atgaaatata
     2161 aatatgtgtt aagtctacta tggctttttt ttgtcgtgtt tattatacgg gctcaagacg
     2221 gaaaaattat tggtcaagta tttgataata cgtctaaaaa accgcttgat tttgccaatg
     2281 tagctctatt tactaatcaa tcaaaagagc cattacaagc aatattgtct gatagtgaag
     2341 gtaagtttat ccttcagaat ttaccctatg ggtcgtttac attgaaagtt tcttttgttg
     2401 gctatgaatc ttttgaaacc tctgtgttgg tgagcaaaga gaaacctaca gctagggtaa
     2461 ataaagttgt attgaaagaa aatgcacaac aaatatcaga ggtacaggta gttggacaac
     2521 gctcgggagt atcttttgaa atagataaaa aagtatttaa tgtggatgaa actgcacttg
     2581 cacaaggttc ttccacaact gatgtgctaa aaaatattcc ttcggtagcg gttgatttag
     2641 aagggaatgt ttcgttgcga aataattcgt cagtagaaat ttggataaat ggcaagccat
     2701 ctggtttgac agatgaaaat agagggcagg tgctagagca aatgccagcc gaaacggtag
     2761 aaaagataga ggttataacc aatccatcag cccgttttaa tccagaggga agtgcaggaa
     2821 ttattaatat tgtattgaaa gaaaaaaatc gtggctccta tttagctagt attaatagca
     2881 atattggtta tgatgagtcg ggggctataa atggttcttt tggtggtaat tatatgtaca
     2941 acggtcccaa atttgatgtg caattgaatg gttctatacg aggaggagaa cgtaaaaatg
     3001 catcatacaa caatcgggca actttggtag cagaagatac tatctcgcat cttaatcaac
     3061 gatcgttatc tgagtcagat atgttcatgg gctttatgag aggaggcgta acctatcata
     3121 tcgacaaaaa aaatgatatt ggacttagtg gttttggtat gttaggagat ttttcgggga
     3181 atagcacaat taattatcat tcattagatg caaatagaga tacccttgaa acgagggaca
     3241 gattagctga taatgcaaat aatcggatgc attacaatgt atctcttgat tacaagcatt
     3301 tttttgataa aactaaccac gagttgtctt cgtctattta ttatgtgcga ggtaatcata
     3361 atagctttaa tgcctacaat acgtcccgtt ttggtaacga tggtgtgtat atcaacgatt
     3421 caaaaacata tcaagagcaa caagctacac gcaatatgta taacattact tggcaggctg
     3481 attactttaa taaatttacc aaagactcta aactcgaaag tggcttaagc tttaattatc
     3541 gcaattcgtt gagcgaagat agaatgtatg attccatctt tactaccaat tcttttgtcg
     3601 aagatgtaga caagtacaat cgatttactt atgtggaaaa tattttttca gcatatacca
     3661 cttactctca aaaaatacaa acatttagtt ttcaacttgg cttgcgtgct gaacaaacaa
     3721 tgactgaggc aaagcagcat tataggtctt attttagttt atttccttcg gtctttctat
     3781 cacaatcttt gccacagaac aacgaattgc agataaatta tacgcgacgt attaatcgtc
     3841 cacgaggttg gcaactcaat gattttgtta accgtactga cgaattaaat ctgtcatacg
     3901 gaaatcctag tttgcgtccc gagtttacta attcatttga atttaattat ataaagaact
     3961 gggagcaagg tcatacgttt tctacctcat tgtattatcg tgctacttct gatgttattc
     4021 agcgtgtaag caagttgcaa aacgaaatta tggaaactac ttacgaaaat attacaacat
     4081 cagaatcagc aggagccgag ttgattttaa aaaacaggtt atttgctaat aaattagatt
     4141 taacctctac actcaatgcc tactactatg cgttagctgc gaatgaaagt tatggcattt
     4201 catcggtaga aaattttaca tggaatgcaa gaatgaatgc gaatattatt attcaacgag
     4261 gttttactac acagctaaca gcgtattatc gtgctccaca agtaaaagtg cagggtcgtg
     4321 atgagcaagt gtatggtatt gatttaggag ctagaaaaac attctttaaa ggggctctca
     4381 atgtgggagt atctgtcaga gatttgcttg attctcaacg aactaccaac attacgtggg
     4441 gcgaaaactt ttatcaagaa agtgcaagtc tctattcagg aagaagttat aggctcaccc
     4501 ttacgtataa ttttggcaac atgaaaaatg gaggcaaaga acagcgtaac aaaaacaaca
     4561 atggttttag tgaagaaaac cttgaatttt aaaaaatatt gctttatctt tgtgtactat
     4621 taattgaaaa aaactaaggt atgaaaaagc ttattttact agcattgggt tttttagtag
     4681 ctacttgcat gggtgtagca caggttggtg tgccggttgt taaatcaaca aatgctccga
     4741 ataaagataa agtaaacgtt tcaactatta cattcaatta tccagctaaa gatattgaaa
     4801 cagcggtatt agaaaaacta aaaaaagaag gtctaacacc acgaaaatct aaatcgaaat
     4861 ttgtcgtttt tgctggagta gaatacttcc ctctttggga taataaattc gatttttatc
     4921 ttcgtatttc aggctctaac aatgcaggta ccgttgaatt attaatgaca caagggtata
     4981 ataattatat caatcctact accccagaag tacaagaaag ggtgaataaa tggttgcttg
     5041 gattggaaaa aaccgtgagt gattatattt atgagcgagc tatggcagag catttgaaag
     5101 agaaaaagtt gattgagcgt accttatcct ctttacaaac gcaacaagat agattaacaa
     5161 atcaatacga taatttaaat aaacaacaag aagcgtttga aaaaaccaaa gtagatgtct
     5221 ctaaagcaga tccaaatacc ttgaatacca aagatatagc caaacagcaa aaacagcaag
     5281 agaaaattgt gaaagaacgt aataagctag aatatagctt gtatgacgta cgttcgagaa
     5341 ttgcagcttc gcaaaaagat ttagacaata gaaacaaagc aattgacgaa ctgaaacaac
     5401 agaaacgcta atggcaagat tgctattatt tcttattccg ttgttgttgg tgtcgtgtgt
     5461 agcacctgct gattataacg attcgttgat acgcgaacag cgtaaagtcc tgtctgctat
     5521 taatgacttg aatacagcta tcaaacaagc caacaaagaa gaaaccaata gtgaactagc
     5581 agaggctgtc aatcaggtag aaaaatctat ccttaaagtg caacagaaag atgcttttag
     5641 aggggaaaca cagttgcgag aggaaatgga ggagttgctt gttttttata agtttgtttt
     5701 aaacgagtat tatccattgc tgatagaaat tcaacacgat gaaggcttaa cgctaactga
     5761 aagaaagcaa gtttccgact ctatttacga taccatacgt gaacaagaaa ttcaacgtac
     5821 aactgcttat gcagaggctc aaaaactgtt tgccgacaag tacaatatat ccatcattca
     5881 tactcagaaa tagaaactac catggaggaa accttgttgc aacttttttt gcgtgtaata
     5941 ggcgaacagc ctacatccgt tgtagcattg cccatcgctg gttctaacag gaaataccta
     6001 cgtatgtgca ccaaaaaaca gtcgtttata ggtgtagtag gtactgtgct cgaagaaaat
     6061 aaagcgttta ttaccattgc taatcagttt gtttccaaag gcttacccgt gccacgtgta
     6121 gtgggtgtgt cagacgatga gatgtgctat cttgttgaag atttgggtga tgtatcgtta
     6181 ttcgattcga ttaagaaagg cagagaaaca ggagagttta gtgcacaaga cattgagcta
     6241 ctaaaaaaaa caattgctta tttaccgtcg ttgcaaattg aaggggcaaa tggtttcgat
     6301 ttttccatct gttttccgca agcagccttt aattaccgta cggtgatgtg ggatttgaat
     6361 tactttaaat attgtttttt aaaacctgct ggtatcgaat gtaacgaaga atggttaga
//
LOCUS       DAOSMY010000043         6319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12438_length_6319_cov_3.713442, whole genome shotgun sequence.
ACCESSION   DAOSMY010000043 DAOSMY010000000
VERSION     DAOSMY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12438_length_6319_cov_3.713442"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLD76_01800"
     CDS             <1..447
                     /locus_tag="PLD76_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOU26773.1"
                     /translation="GETLKKKLRFSNERFTEGLVTAFLLFCMGSMSIIGAIEEGFGKT
                     SDLLLTKSLMDGFSALMLASALGVGVVFSSIPLLLFQGGITLMVWMFAKDIPTIYITE
                     ITAVGGILLIGLGISILEIRKLNIINLLPSLVFICLFLYIRSYFAI"
     gene            448..2580
                     /locus_tag="PLD76_01805"
     CDS             448..2580
                     /locus_tag="PLD76_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOU26774.1"
                     /translation="MNATIISLIAKHLGISEKQVKNTADLLGSGNTIPFISRYRKETT
                     GNLDEVQIGNINELVQYFEELNKRKETILNTIEEQGNLTPELAEKIATCWESNALEDM
                     YLPYKPKRKTKGQAAKENGLEPLAKILMSQSADDPYRIAQRFVTGNVQNNEAAVQGAL
                     DIIAEWINESAIARNRIRQIFAYDAVITANIIKGKEQEGAHYENYFKVKEPLKKCSSH
                     RLLAIRRGEAEGFLRVSINPSDEEVCLTKLTQIFVKNNTPSAQLVATACKDAYKRLLK
                     PSIETEFAHSSKELADKQAIAVFANNLKQLLLASPLGEKRVLAIDPGYRTGCKVVCLD
                     AQGNLLHNETIYPHPPQKETAQAARKIQQLVETYAIQAIAIGNGTASRETETFMQHIR
                     YPLSVDIFVVSENGASVYSASKIARDEFPQYDVTVRGAVSIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQNKLQTALDETVVHAVNLVGVNLNTASEHLLRYVSGLGPNLAKNIV
                     AYRSQNGEFTSREELKKVPRMGEKTFEQAAGFLRIAGAKNPLDNSAVHPESYYVVEKM
                     AKDLNCQLADLIGKKELAQRIQLERYIDQHIGLLSLNDILKELEKPGRDPRKKVSVIT
                     FDQRITTINDVQVGMLLDGIVTNITNFGVFVDIGIKENGLVHISQLANSYVSDPSTIV
                     SLHQAVQVKVIQVDTEKKRIQLSMKTGE"
     gene            2886..3232
                     /locus_tag="PLD76_01810"
                     /pseudo
     CDS             2886..3232
                     /locus_tag="PLD76_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3234..3557
                     /locus_tag="PLD76_01815"
     CDS             3234..3557
                     /locus_tag="PLD76_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU26775.1"
                     /translation="MATNKFKTVSLDTMIDKHIGKHGTEKREKFENELRIDLLGQAIK
                     QARQACNMTQEELGELVGVQKAQISKIENSVKNARFETIMKVFEALGAKVSFKVELNN
                     KKLAY"
     gene            complement(3641..4471)
                     /locus_tag="PLD76_01820"
     CDS             complement(3641..4471)
                     /locus_tag="PLD76_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOU26776.1"
                     /translation="MEIGKYNTLRVVKHVDFGVYLDGGEFGEILLPIRYVPANCEVDD
                     RVDVFVYVDSEDRIIATTEKPFAEVGDFAALQVKSVTKFGAFLDWGLMKDLLVPFSEQ
                     KVYLEEGKLAVVYIYLDEKTNRIVGSAKLEKWVAKTPASFEENDQVEALIAKKTDLGY
                     KAIINNMYLGLIYQNEIFNPVAVGQKVPAFIKQVREDGKIDVRLQRSGAQHVMTEAER
                     ILAKLTDAGGFLPTTDKTAPEEIYATFGISKKSYKKVVGELYKRRLITIEEEGIRLVK
                     "
     gene            complement(4495..6072)
                     /locus_tag="PLD76_01825"
     CDS             complement(4495..6072)
                     /locus_tag="PLD76_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOU26777.1"
                     /translation="MTFQEEIKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIKKTATSDWMEIEKQRGISVATSVMGFEYRDYKINILDTPGHQDFAEDTFRTLTA
                     VDSVIIVIDTAKGVETQTRKLMQVCRMRNTPVMVFVNKMDREGKEPFDLLDEIESELG
                     IAVRPLSWPINMGVGFKGVYNIYKKSLDLYTPNKQVISESIAFNDLSSKELEKHIGEN
                     NAAKLREDIELIEGVYEPFDVNTYLNGSLAPVFFGSALNNFGVKELLDCFVEIAPSPR
                     AVEATERIVKPEENNFSGFIFKIHANMDPNHRSCIAFVKICSGKFERNVNYKHIRHNK
                     LMKFSSPTAFMAQKKETVDEAFAGDIIGLPDTGTFKIGDSLTAGEEIHFKGLPSFSPE
                     MFKYIENADPLKAKQLNKGIDQLMDEGVAQLFVNQYNGRKIIGTVGQLQFEVIQYRLL
                     HEYNAQCKWEPISLYKACWIESDNAEELEQFKKRKFQYMAKDKEGRDVFLADSGYVLQ
                     MAQSDFKNIRFHFTSEF"
BASE COUNT         2030 a         1270 c         1200 g         1819 t
ORIGIN      
        1 ggcgaaactt taaaaaagaa actgcgtttt tccaacgagc gttttaccga aggcttagtt
       61 acggcttttt tgcttttttg tatgggttcc atgtcaatta ttggagctat tgaagaaggt
      121 tttggcaaaa catccgattt gttattaacc aaatccttga tggatggttt ttctgccttg
      181 atgttggctt cggcattggg tgttggtgtg gtattttcat ctataccgct actgctattt
      241 cagggaggta ttacgctgat ggtatggatg tttgcaaaag acattcctac aatttacatc
      301 accgaaataa cggctgttgg gggtattttg ttgattggac taggtatttc cattttagaa
      361 ataagaaaac tcaacatcat caacttactg ccatcgcttg tctttatttg tctattttta
      421 tatatccgtt catattttgc catctaaatg aatgcaacca tcatatcatt gatagccaaa
      481 catcttggca ttagcgaaaa acaagtaaaa aacacagccg acttgcttgg ttcgggcaat
      541 accattccgt ttattagtcg ctaccgcaaa gaaactactg gcaacttaga tgaagtgcaa
      601 ataggcaata tcaatgaatt agtacagtat tttgaggaac taaataaacg caaagaaacc
      661 atcctcaaca ccattgaaga acaggggaat ctgaccccag aacttgctga aaaaatagcc
      721 acttgttggg aaagcaacgc actagaagat atgtatttgc cctacaagcc aaaacgtaaa
      781 accaaagggc aagccgccaa agaaaatggc ttagaaccct tggcaaaaat actgatgagt
      841 caatcggcag acgaccctta tcgcattgct cagcgatttg ttacaggcaa tgtacaaaac
      901 aatgaagcgg ctgtgcaagg agccttggat atcattgccg agtggattaa cgaaagtgcc
      961 atcgcacgta accgcattcg ccaaatattt gcatacgatg ctgtgattac agccaatatt
     1021 atcaaaggaa aagagcaaga aggggctcat tacgaaaatt attttaaggt aaaagagccg
     1081 ctaaaaaaat gtagctcgca ccgcttatta gccattcgta gaggcgaagc tgaaggattt
     1141 ttacgggtat ccatcaatcc gtctgacgaa gaggtatgcc ttacaaaact gacacagatt
     1201 tttgtaaaaa acaacacgcc atcggctcaa ctcgtagcta ctgcctgcaa agatgcgtat
     1261 aaacgtttgc taaaaccgtc tattgaaaca gaatttgccc atagcagtaa agagcttgcc
     1321 gacaaacaag ccattgccgt tttcgccaat aacctgaaac aattgttatt agcatctcct
     1381 ttgggcgaaa aaagggtgct tgccatcgac cctggctacc gaacagggtg taaagtggta
     1441 tgtttggatg ctcaaggcaa tttgctacac aacgaaacca tatatccgca tccaccacaa
     1501 aaagagaccg ctcaggctgc ccgcaaaata caacaattgg tagaaacata cgccatacaa
     1561 gccattgcta taggcaacgg aactgcctcc agagaaacgg aaacgtttat gcaacacatt
     1621 cgctatccct tgtcagtaga tatttttgta gtaagcgaaa atggggcttc ggtatattcg
     1681 gcttctaaaa ttgcccgcga tgagtttcct caatacgatg ttacggtgcg tggagctgta
     1741 tccattgggc gtaggttaat ggatcccttg gctgaattgg taaaaataga cccaaaatcc
     1801 attggcgtag ggcaatacca acacgatgtg gatcagaata aattgcaaac agcattagac
     1861 gaaacggtag tacatgccgt aaatttagtg ggtgtaaatt taaatactgc cagcgaacat
     1921 ttgttgcgtt atgtgtcggg actgggacca aatttagcta aaaatattgt agcgtatcgt
     1981 tcgcaaaatg gcgaatttac cagcagggaa gagctaaaaa aagtgccacg catgggtgaa
     2041 aaaacctttg agcaagccgc aggattttta cgcattgctg gagctaaaaa ccccttggat
     2101 aactctgctg tgcaccccga aagttactat gtggtagaaa aaatggcaaa agacctgaat
     2161 tgtcaattgg ctgatttaat tggtaaaaaa gagctagcac aacgcataca gctcgaacgg
     2221 tatatcgacc aacatatagg tttactttcg ctcaacgaca tcttaaaaga actcgaaaaa
     2281 ccgggtagag acccccgtaa aaaagtcagt gtaatcactt tcgaccaacg cattactact
     2341 atcaacgatg tgcaagtagg catgttactt gacgggattg taaccaatat caccaacttt
     2401 ggtgtgtttg tagatatcgg tatcaaggaa aatggcttgg tgcatatttc gcagttagca
     2461 aacagctatg tatctgaccc aagcaccatt gtatccttgc atcaagctgt gcaagtaaaa
     2521 gtaatacaag tagatacgga aaagaaacgg attcagctca gcatgaaaac gggcgaataa
     2581 agggaatgtg aaacaattat ttcaccaacc gaataccctc ttcctctatg gttatcaaac
     2641 gtcttttgta caattcgcct attccctcct cttgccgtag tttgtattat cgcactacac
     2701 gctgagcgta tgcctattgc atacgtttag tgcataggaa ctcaaccatg ttaaagagct
     2761 tacgtagaac gaggattaca atctatgaag tttcaaacat tgatataaaa caacaaaaaa
     2821 taaaaattta ttacaaaaag cttgtttccc aatagggaaa tatttatctt tgacaaaaat
     2881 tatgaatgaa accaaacttt gacatagaat tattgcctga agcggtagaa tttttagaaa
     2941 accttgacga aaaatcaaga gaaaaattta ttacactatc aaaaaagcac aatttgtaaa
     3001 cgacaacgaa ttattcaaaa agttaaacga tttcatttgg gaatttagaa ctttatacaa
     3061 aggaaaagca tttcgactgt tttcgttttg ggacaaatca aacgggagag aaaccttgat
     3121 tgtggctact cacggaattt taaagaaaac acagaaaaca ccaacaaaag agattaaaaa
     3181 agccgaagaa ataagaaaac aatatttaga atacaagtca aaaagtaaat aatatggcaa
     3241 caaataagtt taaaaccgtt tcgcttgata ccatgataga caaacacatt ggaaagcatg
     3301 gaactgaaaa gcgagaaaaa tttgagaatg agttgagaat tgacctctta ggacaagcca
     3361 taaaacaagc tcgacaagca tgtaatatga cacaagaaga attaggggag cttgtgggtg
     3421 ttcaaaaagc acaaatttca aaaattgaaa acagtgttaa aaatgcaaga tttgaaacta
     3481 ttatgaaagt gtttgaagcc ttgggtgcta aggtaagttt taaggtagaa ctgaataata
     3541 aaaaattagc atactaaaat gcattccatc tggagttctt gcttgccaca caattcattt
     3601 tgtgcgatag cgtaggagga ataaagggaa tgtgaaacaa ttatttcacc aaccgaatac
     3661 cctcttcctc tatggttatc aaacgtcttt tgtacaattc gcctactact tttttgtacg
     3721 attttttgct aatgccaaaa gtggcataaa tttcttctgg agcagttttg tcggtagtag
     3781 gtaaaaagcc gccagcatca gtaagttttg ccaaaatacg ttctgcctct gtcattacat
     3841 gctgtgctcc tgaacgttgc aatcgtacat caatcttgcc atcttcccgt acttgcttaa
     3901 taaaagcagg cactttctgt ccaacagcaa cgggattaaa aatttcgttt tgataaatca
     3961 aacctaaata catattgttg atgatagctt tgtagcctaa atcggttttt ttcgcaatca
     4021 aggcttctac ttggtcgttt tcttcgaatg aagccggtgt ttttgcaacc catttttcga
     4081 gttttgccga acccactatg cggttggttt tttcgtctaa ataaatatac accacagcca
     4141 atttaccctc ttctaaatac actttttgtt cgctaaatgg caccaacaag tctttcatca
     4201 gcccccaatc taaaaatgcc ccaaatttgg ttacgctttt cacctgcaaa gcggcaaaat
     4261 cgccaacttc ggcaaaaggt ttttcggtgg tggcaataat gcggtcttcc gaatctacat
     4321 acacaaacac atctacccta tcgtccactt cgcaattggc aggaacatac cgtatcggca
     4381 acaaaatttc gccaaactcg ccgccatcga ggtaaacccc aaaatcaaca tgtttcacta
     4441 cacgtaaggt attgtatttt cctatttcca tggtatttgt atgtgttata ttttttaaaa
     4501 ttcggacgta aaatggaaac gtatattctt aaaatcgctt tgtgccattt gcaatacata
     4561 acccgaatcg gctaaaaaga catctctgcc ctctttgtct tttgccatgt attggaactt
     4621 gcgtttttta aattgctcta attcttcggc attatcactt tctatccaac aagctttgta
     4681 caaactgatt ggctcccatt tacactgagc attgtactcg tgcaacaaac gatactgaat
     4741 tacttcaaat tgtaattgac caactgtacc aataattttt ctgccattat attggtttac
     4801 aaataattga gccacacctt cgtccattaa ctgatcgatt cctttgttta gctgtttggc
     4861 ttttaatggg tcagcatttt cgatgtactt aaacatttcg ggagagaaac tgggcaatcc
     4921 tttgaaatga atttcttcac ctgcggttag gctgtcgcca attttaaagg tacctgtatc
     4981 gggcaaaccg ataatatcgc ctgcaaaagc ctcatcaaca gtttctttct tttgtgccat
     5041 gaaagcggta ggcgatgaaa atttcatcaa cttattatga cgaatgtgtt tgtagttgac
     5101 attacgctca aacttgcctg aacatatttt tacaaaagca atacagctac gatgattggg
     5161 gtccatattg gcatgaatct taaaaataaa cccagagaaa ttattctctt caggttttac
     5221 aatccgctca gtagcctcta cggcacgtgg cgatggggct atttcaacaa aacaatccaa
     5281 caattctttt accccaaaat tattcaaagc actaccaaaa aatacgggtg ccaaactacc
     5341 attaagatac gtatttacat caaaaggctc gtacacccct tcgataagct cgatatcttc
     5401 acgcaattta gcggcgttat tttctccaat gtgtttttct aactctttgc tcgaaaggtc
     5461 gttaaaggca attgactcag aaataacttg cttgttggga gtatataggt ctaaactttt
     5521 tttgtaaata ttgtacaccc ctttaaaacc aacccccata ttgatgggcc aactcaaagg
     5581 acgaacagca atacccaatt cactttcaat ctcgtcaagc aaatcgaaag gttcttttcc
     5641 ttctctatcc attttattta caaataccat tacaggcgta ttacgcatac gacaaacttg
     5701 catcagttta cgcgtttgtg tttctacccc cttggcagta tcaatcacaa taattacgct
     5761 atctaccgca gtaagtgtac gaaacgtatc ctcggcaaaa tcttggtgac caggcgtatc
     5821 caaaatattg attttataat ctcgatattc gaaccccatt accgatgttg ccaccgaaat
     5881 accccgttgt ttttcaattt ccatccagtc agacgtggca gtttttttga ttttgttcga
     5941 ttttaccgct cccgcaatat gaatagctcc accaaaaagc aagagttttt cggttaaagt
     6001 agtcttacca gcatcgggat ggctgatgat agcaaaagtg cgtcttcgtt ttatttcttc
     6061 ttgaaaagtc atgttatcgt gctaaaattg aggtgcaaag ataataaaaa tccgcttacg
     6121 aaattgtaag aacttgtaaa gtttgcaagt ttataaagtt tgaaagtagt agatagttga
     6181 taataaagaa aagccacacg agagacgggg aacgttgttc gtttagcgtt tatcgttgtt
     6241 cgtgaggtag cggtagtttg gtttgaatag ggaaagctgg agctttcctt ttaacttcga
     6301 catagcgaga cgctatgcc
//
LOCUS       DAOSMY010000044         6292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12500_length_6292_cov_4.187109, whole genome shotgun sequence.
ACCESSION   DAOSMY010000044 DAOSMY010000000
VERSION     DAOSMY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12500_length_6292_cov_4.187109"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="PLD76_01830"
     CDS             complement(<1..1883)
                     /locus_tag="PLD76_01830"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU26778.1"
                     /translation="MWKSEVKKTDIGWIVEMKIPFSALRFPNTNTQVWEINFFRLIKR
                     NGEGITWNFVDKNKNGWLNQAGEMHGICGINPPLRLSFMPYIATYYNDNKFLIKGGLD
                     VKVGLNESFTLDMMLIPDFGQTRADDTYLNLSSVETKYDEKRQFFTEGTELFSKGDIF
                     YSRRIGGQPRNYNKVYSYTKENEILQRNPSETNLYNATKISGHTSSGWGIGMLNAITK
                     KQVAEIKDTLTDVTRYVTTQGVSNYNVMVVNKVIGKESYISWVNTNLLIPEQNYVSNV
                     SGFDFKYMLKDHAFTGDAFVSYNKERELSPISNELGYRANVTISKLKGTFRYEYLATI
                     LSDSYNPTDIGYLENNNIITNTLILYYLNYTPTKSVNEVTSQLAIYYDNLYHPLLYSR
                     LELAWNLKLLFPSFNSIKFYISATPIEKYDHFEPRVVGWKAKEPTAGYVGAEYSSDYR
                     KKLAFFTNIGAWKASQYNKSSQYFTLAPTYRINDKLALTYQFISSFLQNAIGYVDKNN
                     TNDSIFFGRRDISNIENIAQVKYSLSNNSLLNLRLRHYWSNVDHHAYYLLQHDGTFQS
                     TIREANYNTNVSALNADISYTWRFLPGSELSLVWKRYFYLYNQDVASSYVKNLQNVLD
                     YSQV"
     gene            complement(1890..2342)
                     /locus_tag="PLD76_01835"
     CDS             complement(1890..2342)
                     /locus_tag="PLD76_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26779.1"
                     /translation="MKKQLSVLLFLLCVWSVVLAQHRKEFTIVQTSIPPKIDAVPTDT
                     VWDAAYSIRDFIQQSPTNGGVPSQHTEVRMLYDNEAIYVLAMLYDTKPDSIYAEIGDR
                     DSGDEANADLFSVEINPYNDGLNATEFMVSAAGVQMDSKNDLQTMNKS"
     gene            complement(2635..3348)
                     /locus_tag="PLD76_01840"
     CDS             complement(2635..3348)
                     /locus_tag="PLD76_01840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOU26780.1"
                     /translation="MENNQEVYAMPRIGEKAPVFKAVTTQGEINFPADYSGSWVILFS
                     HPADFTPVCTSEFMTFATMEKQFNQANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMK
                     DVEVTFPLIEDITMEVAKKYGMMMPGESNTKAVRAVFVIDPQGIIRTIIYYPLSLGRN
                     FDELYRVILALQTADEFKVATPADWRPGDDVIISPAGSCGTAKDRMDGKEDGLECKDW
                     FFCTKKLDKDLVLGKILKK"
     gene            complement(3370..3795)
                     /locus_tag="PLD76_01845"
     CDS             complement(3370..3795)
                     /locus_tag="PLD76_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOU26781.1"
                     /translation="MEKSIISLQQLIANSGMKVTPQRLAVFEAVMSMRTHPTADAIIA
                     RVQEQHPHIAVGTIYNVLDALVDKKLIVRVKTDKDSMRYDAVLDDHHHLYCSQTGEIV
                     DYYNQELSLILTNYFKERGIPNFEIQDIKLQINGIYKPQ"
     gene            4007..>6292
                     /gene="topA"
                     /locus_tag="PLD76_01850"
     CDS             4007..>6292
                     /gene="topA"
                     /locus_tag="PLD76_01850"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU26782.1"
                     /translation="MGKNIVIVESPAKAKTIEKFLGKDYHVVSSFGHIRDLKKKDISI
                     DFNNNYSPIYEVPTDKKKLVSELKKKASEADMVWLASDEDREGEAIAWHLFEVLSLTE
                     DKTKRIVFHEITKDAIINAIQTPRKIDKALVDAQQARRVLDRIVGFELSPILWKKIKP
                     SLSAGRVQSVAVRLIVEREREITTFKSEANYRITAVFITKDTQGKTVELQAELPKRFK
                     TKEEANRFLEVCKDAQFNIEDISKKPSKKSPAAPFTTSTLQQEAARKLGYSVAQTMMV
                     AQKLYENGLITYMRTDSVNLSALAINTAKAEIIHFAGEQYVKTRQYATKSKGAQEAHE
                     AIRPTYMNQHTIEGNRQEKFLYELIWKRTIASQMADAEIEKTTVTIGISTSEERFIAS
                     GEVLTFDGFLKVYMESNDDEDTTEDTKMLPPLHVHDTLQYKKILSTERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNREYVEKGDKEGEKRTYNLLTLEKGTITDIIK
                     EEIVGTEKSKLFPTDIGSVVTDFLLANFASVVDYNFTAKVEKEFDIVASGKLEWQKAI
                     DKFYKGFHTLVEDSLQNSTKKVGERLLGNDPTTGKPISVKIGRYGAIAQIGLTTEEEK
                     PQFAALKKEQSIETITLEEALELFKLPRTVGEFEEKEVTIAVGRFGPYVKHASAFYSI
                     PKEIDPLKITLSGAIDLIEKKREDDKNKVLKTFAEDASIQLLNGRYGAYIKQNKNNYK
                     IPKSKKAEELTYEDCLEIIKSE"
BASE COUNT         2171 a         1189 c         1026 g         1906 t
ORIGIN      
        1 acttgagagt aatctaatac gttttgcaaa tttttgacat aactactagc aacatcttgg
       61 ttgtacagat aaaaatatct tttccaaacc aatgatagtt cgcttccggg caaaaaacgc
      121 cacgtgtaac ttatgtcagc atttaatgca ctaacattgg tattataatt ggcctccctg
      181 atagttgact gaaatgtgcc atcgtgctgt aaaagataat atgcatgatg gtctacattt
      241 gaccaataat gacgtaatcg aagatttagc aaactattgt ttgacaaaga atatttgact
      301 tgtgcaatat tctcaatatt tgaaatatct cttctgccaa aaaagattga atcattggtg
      361 ttatttttat caacataccc tatagcattt tgcagaaaac tacttataaa ctgatacgtg
      421 agtgcaagct tatcattaat gcgatacgta ggagccaatg taaaatattg ggaactttta
      481 ttgtattgag aggctttcca agcacctata tttgtaaaaa aagctaattt tttacgataa
      541 tcagaagaat actctgctcc cacatagcca gcggtaggtt cttttgcttt ccaacctaca
      601 acacgtggtt caaaatggtc gtacttttcg ataggtgttg cactaatata aaactttatt
      661 gaattaaatg atggaaataa caattttaaa ttccatgcca actctaatct actatacaat
      721 aatggatgat aaagattatc gtaataaata gccagttgac tagtaacttc atttactgat
      781 ttggtaggag tataatttaa ataatacaaa attaacgtat tcgtaatgat attattattt
      841 tcaagatacc ctatatccgt aggattatat gaatcggata aaatagttgc gaggtattcg
      901 tatctaaagg tccctttcag ttttgagata gtaacatttg ccctataacc cagctcgttg
      961 gatataggag aaagctctct ttccttatta taactcacaa atgcatctcc agtaaacgca
     1021 tgatctttca acatatattt aaaatcaaaa ccggacacat tcgacacata attttgctca
     1081 ggaatgagca aatttgtatt gacccaacta atatacgatt ctttcccaat aactttattt
     1141 actaccatca cattgtaatt agatacccct tgtgtagtta catatcttgt aacatcggta
     1201 agagtatctt ttatttcagc tacttgtttt tttgtaatag catttaacat acctattccc
     1261 caaccagaag aagtatgtcc agaaattttc gttgcattat ataaattcgt ttcagatggg
     1321 tttctttgta gtatttcatt ttcctttgta tatgaatata ccttgttata gttgcgaggt
     1381 tgacctccta ttcgacgtga ataaaatata tcgcctttgc taaatagctc ggtaccctca
     1441 gtaaaaaatt gtcgtttttc atcatacttt gtttccactg acgacaaatt taaatacgta
     1501 tcatcggctc gtgtttgccc aaaatcggga atgagcatca tatcgagagt aaaactttca
     1561 ttgagtccca cttttacatc caatcctcct tttatcaaaa acttattatc attatagtat
     1621 gtagcaatat agggcataaa cgaaagccgt aatggaggat taataccaca aatgccgtgc
     1681 atctcgccag cttgattaag ccagccgttt ttatttttat cgacaaaatt ccatgttata
     1741 ccctctccat tacgtttaat cagacgaaaa aaattaattt cccaaacctg tgtatttgta
     1801 ttaggaaaac gtaatgcaga aaaaggaatt ttcatttcca ctatccaacc gatgtcggtt
     1861 ttcttaactt cacttttcca cacagcatct caacttttgt tcatggtctg taaatcgttt
     1921 ttcgaatcca tctggactcc tgccgccgaa accataaatt cagtagcatt gagtccatcg
     1981 ttataggggt ttatctctac cgagaaaagg tcggcattag cctcatcgcc cgaatcccta
     2041 tcccctattt cagcataaat agaatcaggc ttagtgtcat acagcattgc caacacatat
     2101 attgcctcat tatcatatag cattcttacc tctgtgtgtt gcgaaggcac accaccgtta
     2161 gtaggactct gctgaataaa atcgcgaata ctatatgctg catcccatac agtatcggta
     2221 ggaacagcat caattttagg cgggatactt gtttgcacaa tggtaaactc ttttctgtgt
     2281 tgagccaata caactgacca tacacacaat aaaaacagta gcacactaag ttgttttttc
     2341 atagacgtaa cataaggtat aattatcatc ccaaagaaaa tagaataaac tgacaacaaa
     2401 aaaaatatta caccaacgta ctactattta tgcaaacgta gaaaaaaata ctctttatga
     2461 taatcttctg acacaagaat attgaaaaaa ggataactat aacacgcaac caacataaaa
     2521 gatcgttgca acatattgta taatttgatc atactttagc ttacaactct cttttatgca
     2581 aaaaaagagc cactcatact gagtagctct atccttttta ggaaagttga aatactattt
     2641 cttaagaatt ttacccaaca caaggtcttt gtcaagtttt ttggtacaga agaaccagtc
     2701 tttacattcc agtccatctt ctttaccatc cattctatct ttggcagtac cacacgaacc
     2761 tgctggtgaa ataatcacat catcacctgg acgccaatca gcaggagtcg ctaccttaaa
     2821 ctcatcagct gtttgcaatg ccaatatcac tctatacaac tcatcaaagt tgcgtcctaa
     2881 gctcaaaggg taataaataa ttgttcggat aattccctgt gggtcaatta caaatacagc
     2941 tcttaccgct tttgtattgc tttcgccagg catcatcatg ccatattttt tggctacttc
     3001 catcgtaata tcttcaataa gaggaaaagt tacctctaca tctttcatcc ctttgtactc
     3061 aattttttct ttgatagtac gtagccatgc aatatgacta tacaaaccat cgactgaaag
     3121 tcctaccaac ttacaatttg cctgattaaa ctgtttttcc atagtagcaa atgtcataaa
     3181 ttctgacgta catacaggag taaaatcggc tggatgacta aataaaatta cccaactacc
     3241 gctataatca gcagggaaat taatttcacc ttgggtagta acagccttaa aaacaggagc
     3301 tttttcgcca atacgtggca tagcatacac ttcttgattg ttttccataa tctttttgtt
     3361 tttttattat tattgtggtt tatatattcc gtttatttgt aatttaatat cttgtatttc
     3421 aaaattaggt atccctcttt ctttaaaata atttgtaagt atcaaactta attcttgatt
     3481 ataataatca actatttctc ctgtttgcga acaatacaag tgatgatgat cgtccaaaac
     3541 agcatcatac cgcatgctat ctttgtcggt ttttacccgt acaatcaatt ttttatctac
     3601 caaggcatct aacacattat atatagtgcc cactgctata tgaggatgct gctcttgaac
     3661 acgtgcaata atggcatcag ctgtggggtg agtcctcata gacataacag cttcgaatac
     3721 cgctaatcgc tggggagtta ctttcatccc gctatttgcg atgagttgtt gtaatgaaat
     3781 tattgatttt tccacttata cttattattt attaggaata attcttagtt aagaacaaaa
     3841 ataatctgta tttttcaatt aaccaagtat ttttgcatta attttcacac ttttctgtaa
     3901 aaaccataaa aaacaactat ttatatcgaa aaaatagttt tatcaatatc aattttatta
     3961 cttttgcacc gaattttgtt aattaatctg ctaacttggt acaaaaatgg gaaagaatat
     4021 tgttattgtg gaatctccgg caaaagccaa aacgattgaa aaatttcttg gaaaagatta
     4081 ccatgttgtg tctagttttg gtcatatacg cgatttgaag aaaaaagaca ttagcataga
     4141 ttttaacaat aattactccc ctatttacga ggttccaacc gacaaaaaga aacttgtaag
     4201 cgaattaaaa aagaaagcct ctgaagccga tatggtatgg ttggcatccg atgaagaccg
     4261 cgagggagaa gccattgctt ggcatttatt tgaagtatta tcgttgaccg aagacaaaac
     4321 caaacgaatt gtatttcacg aaattaccaa agacgcaatt atcaatgcca tacaaacacc
     4381 cagaaaaatt gacaaagcac ttgtagatgc acaacaagca cgtagggtgc tcgatcgcat
     4441 tgtaggtttt gaactttcgc ccatattgtg gaaaaaaatt aaaccttcgt tgagtgccgg
     4501 tcgtgtacaa tcagtagctg ttcgtttaat cgtagaacga gagagagaaa taactacttt
     4561 caaatctgaa gcaaattacc gcattactgc tgtgtttatt accaaagata cacaaggaaa
     4621 aacggtagag ttacaagccg aattacccaa acgttttaaa accaaagaag aagcaaaccg
     4681 ctttttagaa gtgtgcaaag acgctcaatt caacattgaa gacataagca aaaaaccgag
     4741 caaaaaatcg cctgctgccc catttacaac atctacattg cagcaagaag ctgccagaaa
     4801 acttggctac tcggtagcac aaaccatgat ggtagcacaa aaattgtacg aaaatggact
     4861 tattacgtat atgcgtaccg attctgtaaa cctttctgcc ttggctatta atacagctaa
     4921 agcggaaatt atacattttg ctggagagca atacgtaaaa actcgccaat acgctaccaa
     4981 atcgaaagga gcacaagaag cacacgaagc catacgtcct acctacatga atcaacatac
     5041 catagaagga aatcgacaag aaaaattctt gtatgagttg atttggaaac gtacaattgc
     5101 atcgcaaatg gctgatgctg aaatagaaaa aacaactgta actattggta tctctactag
     5161 cgaagaacga tttatagcta gtggcgaggt gctaactttt gatggtttct taaaagtgta
     5221 tatggagtcg aatgacgatg aagatacaac agaagataca aaaatgctac ctccactgca
     5281 tgtacatgac actttacagt acaaaaaaat acttagtacc gaacgattta cacaacgacc
     5341 accacgatac acggaggcta gtttggtacg taaattagaa gaattaggca ttggacgacc
     5401 atctacctac gcacctacta tcagtacaat tcaaaataga gaatacgtag aaaaaggaga
     5461 taaagaaggt gaaaaaagaa cgtataattt gcttacatta gaaaaaggta caattacaga
     5521 tatcataaaa gaggaaattg ttggtacaga aaaatcaaaa ctttttccaa ccgatatagg
     5581 ttcggtcgta acagattttt tactagccaa ctttgcttcg gttgtagatt ataattttac
     5641 agctaaagta gaaaaagaat ttgatatagt tgcctcaggg aaattggaat ggcaaaaagc
     5701 aattgacaaa ttttacaaag ggtttcatac tcttgttgaa gattcgttgc aaaattccac
     5761 taaaaaagta ggagaaagac ttttgggaaa cgacccaaca acaggaaaac cgatatctgt
     5821 aaaaattgga cgctatgggg ctatagccca aattgggtta actaccgaag aagaaaagcc
     5881 acaatttgct gcactaaaaa aagaacaatc tatcgagacg attactctcg aagaggcatt
     5941 agaattattt aaactaccac gaactgtggg tgagtttgag gaaaaagaag taacaatagc
     6001 cgttgggcgt tttggtcctt acgtaaaaca cgcctctgca ttttattcta tccccaaaga
     6061 aattgaccct ttaaaaataa cactgtcggg agcaatcgat ttgatagaaa agaaacgcga
     6121 agacgacaaa aataaagtac taaaaacatt tgcagaagat gcctctatac aactattaaa
     6181 tggacgttat ggtgcgtaca taaaacaaaa taaaaacaat tacaaaatac caaagtcgaa
     6241 aaaagcagaa gagctcacat acgaagattg cttagagatt attaaatcag aa
//
LOCUS       DAOSMY010000045         6138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12909_length_6138_cov_4.057702, whole genome shotgun sequence.
ACCESSION   DAOSMY010000045 DAOSMY010000000
VERSION     DAOSMY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12909_length_6138_cov_4.057702"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..195
                     /locus_tag="PLD76_01855"
     tRNA            114..195
                     /locus_tag="PLD76_01855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Leu,seq:tag)
     gene            291..1643
                     /gene="tig"
                     /locus_tag="PLD76_01860"
     CDS             291..1643
                     /gene="tig"
                     /locus_tag="PLD76_01860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU26783.1"
                     /translation="MNVSQNNIDQVNAVLSIQVKKADYTEKVEKSLKDYRKKANMPGF
                     RPGTVPLGLIKKMYGKAILAEEINKLVSESLYDHIQKNNIPVLGEPLPSENAPVPNFD
                     TQEDFDFSFDIALAPEINLNLTTKNKVTLYNIAVSDEMVAKQVDAYKGRFGKHAPADK
                     VEEKDVVKGELVELLPNGDINENGIKVENALLSPAYMKDKKEQAKVIGLALESQFRFN
                     PAKAFESETEIASLLQVSKEIAADCKADFLFTIKDITRYVEAEMNQDLFDAALGKDVV
                     SSEAEFIDKIKESLKAQFAVDSEFKLSIDMKELVVKQLEGLAFPDVFLKRWLKVSNEN
                     LTEDAIEQEYPKMIEDLKWQLAKDKVAKENELKLEKEDVEAVAKQAAKAQFAQYGMMN
                     VPDDVLDGYVKDMLKNKDAAKNITERAVEQKTIDALKTKVTIVSKEISFEDFNKLFEN
                     "
     gene            1790..2458
                     /gene="clpP"
                     /locus_tag="PLD76_01865"
     CDS             1790..2458
                     /gene="clpP"
                     /locus_tag="PLD76_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOU26784.1"
                     /translation="MNTEFKKYATKHLGMNGLALDKYVDITSSYISPTIIEERQLNVA
                     QMDVFSRLMMDRIIFLGTQVDDYTANVIQAQLLYLDSADPGKDISIYLNTPGGSVYAG
                     LGIYDTMQFISSNVATICTGMAASMGAVLLTAGTAGKRSALKHSRIMIHQPMGGAQGQ
                     ASDIEITAREIQKLKKELYTIIAEHSGNTYERIEQDSDRDYWMTAEEAKAYGMIDEVL
                     VKQK"
     gene            2479..3699
                     /gene="clpX"
                     /locus_tag="PLD76_01870"
     CDS             2479..3699
                     /gene="clpX"
                     /locus_tag="PLD76_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU26785.1"
                     /translation="MSKKDDKCSFCGRGRQEAPLLMQGQTGNICNDCAEQAATIAKET
                     FSKQTDKYQLDKQTLPKPQELKAFVDQYVIGQDDAKKNLCVAVYNHYKRLLQKNTHDD
                     VEIEKSNIILVGATGTGKTLLARTIAKKLHVPFTIVDATVLTEAGYVGEDIESILTRL
                     LQVADYDVKAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSIVNVP
                     PQGGRKHPDQKMIAVNTQNILFICGGAFDGIEKKIAARLNTQIVGYAAAKITHQIDKD
                     NLLQYISPQDLKAFGLIPEIIGRLPILTYLNPLDREALRLILTEPKNSIIKQYKKLFA
                     MDDVVLEFDKDVLDYIVDKSIEYKLGARGLRSIAEMIMVDYMYDIPSSNEHQLRIPLA
                     IAKEKVEKGRMTPV"
     gene            3805..5994
                     /gene="recQ"
                     /locus_tag="PLD76_01875"
     CDS             3805..5994
                     /gene="recQ"
                     /locus_tag="PLD76_01875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOU26786.1"
                     /translation="MSTSLELTQELKKNFGFDSFKGNQEAIIKNLLAGNDTFVLMPTG
                     GGKSLCYQLPSLMMEGTAIVISPLIALMKNQVDAMKAFSEEDGVAHFLNSSLSKAAIE
                     DVKNDVISGKTKLLYVAPESLTKEENILFLKKVKISFYAVDEAHCISEWGHDFRPEYR
                     RIRPLINEIGSAPVVALTATATPKVQHDIQKNLGMVNATVFKSSFNRENLYYEVRTKN
                     QHIDKEIIKYIKSQHGKSGIIYCLSRKKVEELAETLCLNGINALPYHAGMESAVRTAN
                     QDSFLMEKVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGQC
                     ITFYSNKDLQKLEKFMQGKPISEQEIGKQLLLETAAYAESSVCRRTLLLHYFGEEYTQ
                     DNCGSCDNCLNPKKQMEAQDLLCTVLETILSVKEKFKAEHIVDVIVGKETAEVIDYKH
                     QDLEVYGTGESEKPTVWHAVIRQGMLSGYIDKEIENYGLLKVTKSGRAFLEKPTSFKI
                     TKDNDFEEEDDDDATIRRSGGGTGAADEVLFSMLKDLRKKMSKRLEVPPFVIFQDPSL
                     EAMATTYPVTMEELQNIPGVGAGKAKRYGEDFLRLIKTHVQENEIERPVDLRVRTVAN
                     KSKLKVFIIQAIDRKIALDDIAISKGLEFPELLDEIEAIIYSGTKINIDYFINEVMEE
                     DHILDIYEYFRESQTDEVETALAELGSDYTEEEIRLIRIKFVSEIGN"
BASE COUNT         2197 a          918 c         1241 g         1782 t
ORIGIN      
        1 agtacgtgct acaaacgatg tattaaaaga aataaacgca ttataataaa aaaaacaggc
       61 gggaagcatt gataaataaa aaaagagttt tatctttgtc cccgctaagt tttgcggctg
      121 tggcgtaatt ggtagccgcg ccagacttag gatctggtgc cgcgaggcgt gggggttcga
      181 gtcccttcag ccgcacaaca aaaaagaacc gttgtcttag caaacacaac ggtttttttt
      241 gcctataaca aaaagtattt tgctgaataa taaattaatt tcgtagtaca atgaatgttt
      301 cacaaaacaa catcgaccaa gtaaatgccg tactttcaat tcaagtaaaa aaagcggatt
      361 atactgaaaa agtagaaaaa tcattaaaag attatcgtaa aaaagcaaat atgcctgggt
      421 ttagaccagg aactgtgcct cttggattaa tcaaaaagat gtacggaaaa gctattttgg
      481 cagaagaaat taataaactt gtttcagaat cgctatacga tcatatccaa aagaataata
      541 tacctgtttt gggagagcct cttccatcgg aaaacgcccc tgtgccaaat tttgacacac
      601 aagaggattt cgatttttct tttgatattg ctctggctcc tgaaatcaac cttaatttaa
      661 caaccaaaaa taaggtaaca ttatacaata ttgctgtaag tgacgaaatg gttgccaaac
      721 aagtagatgc ttacaaagga cgttttggaa aacacgctcc agcggataag gtagaagaaa
      781 aagatgtagt aaaaggcgaa ttagttgagt tattgcctaa tggcgatatc aacgaaaatg
      841 gtattaaagt agaaaatgct ctattaagtc ctgcgtatat gaaagacaaa aaagagcaag
      901 ctaaagttat tggattagct cttgagtcgc aatttaggtt taatccagct aaagcatttg
      961 aaagcgaaac agaaatagcc tctttattgc aagttagtaa agaaattgct gccgattgca
     1021 aagctgattt cttgtttacc atcaaagata ttacacgcta tgtcgaggct gaaatgaatc
     1081 aagatttatt tgatgcagct cttggaaaag atgtggtatc ttctgaggct gaatttattg
     1141 ataaaattaa agaaagctta aaagctcaat ttgctgttga tagtgagttt aaactgtcta
     1201 ttgacatgaa ggagttggtg gttaaacaac ttgaaggctt agcttttcct gatgtattct
     1261 taaaaagatg gttgaaagtg agcaacgaaa acctgaccga agacgctatt gaacaagaat
     1321 atcctaaaat gatagaagat ttgaaatggc aattagcaaa ggataaagtg gcgaaagaaa
     1381 acgaattgaa attagaaaaa gaagacgtag aagcagtagc caaacaagca gctaaagctc
     1441 aatttgctca atatggcatg atgaatgtgc ctgatgatgt acttgatggc tatgtaaaag
     1501 atatgcttaa aaacaaagat gctgcaaaaa atattaccga acgtgccgta gaacaaaaaa
     1561 caattgatgc cctaaaaaca aaagtaacaa ttgtttcaaa agaaatatca ttcgaagatt
     1621 ttaacaaact gtttgaaaat taagcatata ggatataaaa aatactaaaa aaactttctt
     1681 ttaaatttgg gagtgtaatt tgtttgtttt aaaacaaaaa aaaattatac ctttgtttcc
     1741 ctgatttaaa agaaagtttt tttatgtacc atataatttg gaaaacaata tgaatacaga
     1801 atttaaaaaa tatgcaacaa agcatctcgg aatgaacgga ttggctttgg ataaatatgt
     1861 ggatattaca agtagctata tatcgccaac tattattgaa gaaagacaac tcaatgtcgc
     1921 acaaatggat gttttttcac gtttgatgat ggacagaatt atctttttag gaacacaagt
     1981 ggacgattat actgccaatg taattcaggc tcaattgttg tatcttgatt ctgccgatcc
     2041 agggaaagat atttctattt atcttaatac acctggaggt tctgtgtatg caggcttagg
     2101 tatatacgat accatgcagt ttatttctag caatgtagct accatttgta caggcatggc
     2161 agcctctatg ggtgctgtac tacttaccgc tggtactgca ggcaaacgtt cagcattaaa
     2221 gcactcccgt attatgattc accaaccaat gggtggagct caaggtcaag cttcggatat
     2281 tgaaattact gcccgtgaaa ttcaaaaact caaaaaagaa ttatacacca ttattgccga
     2341 acattcgggc aatacatatg aacgtatcga acaggactct gaccgtgact attggatgac
     2401 ggcagaagaa gcaaaagcct atggtatgat tgacgaagtg ttggtaaaac aaaaataaga
     2461 gcaagtaggt ataaagttat gtcaaagaaa gacgataaat gtagtttttg tggacgagga
     2521 cgtcaggaag cgcctctact gatgcaagga caaacaggaa atatatgcaa cgattgtgcc
     2581 gaacaagctg ctaccattgc caaagagact ttttctaaac aaacggataa gtatcaactc
     2641 gataaacaaa ccttgcctaa accacaagaa ttaaaggcat ttgttgatca gtatgtaatt
     2701 ggacaagatg atgcaaaaaa gaatctatgt gtagccgttt acaatcacta taaacgattg
     2761 ttacaaaaaa acacacatga tgatgtagag attgaaaaat cgaacatcat tttagttgga
     2821 gctacaggca caggaaaaac cttgttagca cgcactattg ctaaaaaact gcatgtccct
     2881 tttactattg tagatgcaac tgttttaacc gaagcagggt atgttggtga agatatcgaa
     2941 agtatcttaa ctcgcctatt gcaagtagct gactatgatg tgaaagctgc tgaaaaaggt
     3001 atcgtattta ttgacgaaat agataaaatt gctcgcaaag gagataaccc ctctattact
     3061 cgcgatgtga gtggtgaggg cgtacaacaa ggattactaa agctccttga aggttctatt
     3121 gtaaacgtac ctcctcaagg aggacgaaaa catcctgacc aaaaaatgat tgccgtaaat
     3181 actcaaaata ttttatttat ttgtggggga gcatttgatg gtattgagaa aaaaattgca
     3241 gcacgattaa atactcaaat agtgggatat gcggctgcaa aaataacgca tcaaatagat
     3301 aaagataatt tacttcaata tatttcacca caagacttaa aagcttttgg attgataccc
     3361 gaaataattg gaagattacc tattttaacg tatctcaatc cattagatag agaagcattg
     3421 agattaatat taaccgaacc aaagaattct attatcaagc aatataaaaa attgtttgct
     3481 atggatgatg ttgtattaga gtttgacaaa gatgttcttg attatattgt agataaatct
     3541 attgaatata agctgggtgc tagaggttta cgctctatcg ctgagatgat tatggtagat
     3601 tatatgtatg atataccttc atcaaatgaa catcaattac gcattccttt ggcaattgcc
     3661 aaagagaaag tggagaaagg aagaatgact ccagtgtaat ttattcaaaa tttttctcgc
     3721 tttcatttga ttattaaaaa caagttatta catttgtttt attatacaaa caagagcaac
     3781 tagttttaat cttacattat tgtaatgtca acatctttag aacttacaca agaattaaaa
     3841 aagaattttg gattcgattc ttttaaaggg aatcaagaag ccattattaa aaatctgtta
     3901 gcaggtaatg atacatttgt attgatgcct actgggggag gtaagtcttt gtgttaccag
     3961 ttaccgtcgc ttatgatgga aggaacagct atcgttattt ctccacttat cgctttgatg
     4021 aaaaatcaag tagatgcaat gaaagcattt agcgaagaag atggcgtggc tcatttttta
     4081 aattcatcac tatcgaaagc agctatcgaa gatgtgaaaa atgatgttat ttctggcaaa
     4141 acgaaattgt tgtatgtagc ccctgaatct cttaccaaag aagaaaatat tttatttctg
     4201 aaaaaagtaa aaatttcttt ttatgcagtt gatgaggctc attgtatttc ggaatggggg
     4261 cacgatttta gaccagaata ccgcagaatt cgtccgttaa ttaatgaaat aggttcagct
     4321 ccagtggtag ctcttactgc aacggctaca cccaaagtgc aacatgatat acaaaaaaat
     4381 ttaggaatgg taaatgctac tgtttttaaa tcgtctttta atagagaaaa tttgtattat
     4441 gaggtacgca caaagaatca gcatattgat aaagaaatta ttaaatatat caaatcgcaa
     4501 catggtaagt cgggtattat ttattgttta agtcgtaaga aagtagaaga attagctgaa
     4561 actctctgtt taaatggaat aaatgccttg ccataccatg cgggaatgga atctgctgtt
     4621 cgtacagcca atcaagatag ttttttgatg gaaaaagttg atgtaattgt ggcaacaatt
     4681 gcatttggaa tgggtattga taagcccgat gttcgttttg taatacatta tgatataccc
     4741 aaaagcctcg aaggttatta ccaagaaaca ggtagagctg gtagagatgg aggtgaaggt
     4801 caatgcatta cgttttactc aaataaagac ttgcagaaat tagaaaaatt tatgcaagga
     4861 aaacctatat cagaacaaga aattggcaaa caactcttgt tagaaacggc agcttatgct
     4921 gagtcgtctg tatgtagaag gacattattg ttacattatt tcggagaaga atacacacaa
     4981 gacaattgtg gtagttgtga taattgtttg aaccctaaga agcaaatgga agcacaggat
     5041 ttattatgta cagttttgga aacgatcctt tcagtgaaag aaaaatttaa ggcagagcat
     5101 attgtagatg taatcgttgg gaaagaaacc gcggaagtga ttgattataa acaccaagat
     5161 ttggaagtat atggtacagg cgaaagcgaa aagccgactg tttggcatgc tgttatacga
     5221 caaggtatgt tatctggcta tatcgacaaa gagatagaaa attatggatt gttgaaggtt
     5281 accaaaagtg gtcgtgcttt tttagaaaaa cctacaagct ttaagataac aaaagataat
     5341 gattttgagg aagaagatga tgatgatgca accataagaa ggtcgggtgg aggcactgga
     5401 gctgctgatg aggtattgtt ttctatgctc aaagacttac gaaagaagat gtcgaagaga
     5461 ttggaagttc cgccttttgt cattttccaa gacccttcgt tggaggcaat ggctacaacg
     5521 tatccagtta ctatggaaga gttgcagaat ataccaggag taggtgcagg taaagccaaa
     5581 cgctatggag aagatttttt gcgtttgatt aaaacacatg tgcaagaaaa tgaaattgaa
     5641 cgccctgttg atttacgggt gcgtacagtg gctaataaat ctaaactaaa agtctttatc
     5701 attcaagcta ttgacagaaa aattgcatta gatgatatcg ctatatccaa ggggttagag
     5761 tttcctgagt tgttggatga gattgaagcc atcatttatt cgggtactaa aatcaatatc
     5821 gattatttta tcaacgaggt aatggaggaa gatcatatat tggatatata cgagtatttt
     5881 agagagtctc aaacagacga agtggaaaca gctcttgccg aattgggaag cgattataca
     5941 gaagaagaaa ttcgattgat acgcattaag tttgtgtctg aaataggcaa ttaacttctt
     6001 gcagaaataa gaagtttaaa gagatagcat ttatttgttg tctctttttt tttgcaaaaa
     6061 aatgggacaa cgaaaagttg tcccacaaaa gaaaaggctc actacttcat tttctttttt
     6121 atagttttag acttagtc
//
LOCUS       DAOSMY010000046         6135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12917_length_6135_cov_3.027138, whole genome shotgun sequence.
ACCESSION   DAOSMY010000046 DAOSMY010000000
VERSION     DAOSMY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12917_length_6135_cov_3.027138"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /gene="msrA"
                     /locus_tag="PLD76_01880"
     CDS             complement(<1..401)
                     /gene="msrA"
                     /locus_tag="PLD76_01880"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOU26787.1"
                     /translation="MIEKAYFASGCFWGTEYYFMQLPRIATQVGYMGGLTFAPTYREV
                     CTGDTGHIETVEIVYDNEQVEYETLVKLFFETHNFSQIDGQGPDIGSQYVSVIFYTNE
                     KQRDIATYYITLLQTKGYFVATQLRQAEEFWV"
     gene            complement(401..754)
                     /locus_tag="PLD76_01885"
     CDS             complement(401..754)
                     /locus_tag="PLD76_01885"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004036.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HOU26788.1"
                     /translation="MNKLTKEEEHIIVHKGTELPFSGEYVHTKEDGIYSCRRCDTPLF
                     LSDDKFDSECGWPSFDDAIKEAVLEIPDMDGRRTEIVCANCHGHLGHVFEGEGFTEKN
                     VRHCVNSLSIKLMKK"
     gene            complement(783..1676)
                     /gene="dapA"
                     /locus_tag="PLD76_01890"
     CDS             complement(783..1676)
                     /gene="dapA"
                     /locus_tag="PLD76_01890"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU26789.1"
                     /translation="MERNKFIGMGVALVTPFKTDGSVDFSALARLVDYQLKNGTDYLV
                     VLGTTAETPTLSAEEQLEITQFIVEKVNGKLPIVLGMGGNDTRALVQKISTANLNGVD
                     AILSVTPYYNKPTQEGIYQHYAAVAKSTKLPIILYNVPGRTGVNMSVETTLRLANDFS
                     NIIATKEASGNIAQVDAIIKNKPKDFLVISGDDAITFPLITLGASGVISVIGNAFPCE
                     FSKMVRLALAGDYQTALTIHHGFTELIELLFVDGNPAGVKSILSAMGYLENQLRLPLL
                     PTRITTYEKMRNILLNMNIKC"
     gene            complement(1790..2329)
                     /locus_tag="PLD76_01895"
     CDS             complement(1790..2329)
                     /locus_tag="PLD76_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26790.1"
                     /translation="MNQLQNILFKLRRYFLLKFHKKQWRDKKYLSFDNISSVALLYYI
                     DDYSKYEIVYKNIKDFTAMGKTVFSVLFVKKGVLAEEKRINMLLFTQNDCSWLCVPND
                     NIMLDALNISADILIDISLKDSIPLQYIVANSQVGCKVAMKKENKFLYDLMIEMKEEQ
                     VNEDKLLETIAFYLKQIKS"
     gene            complement(2374..4389)
                     /gene="ligA"
                     /locus_tag="PLD76_01900"
     CDS             complement(2374..4389)
                     /gene="ligA"
                     /locus_tag="PLD76_01900"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU26791.1"
                     /translation="MNEIHQKILHLRKQLHQYNYEYYVLSQPTISDIEFDFLLKKLQA
                     LEELHLEYYDSNSPTQRVGSDSTTSFVQVSHQYPMLSLSNTYSEGEVRDFYERVSKSI
                     DEPFELVCELKFDGTSISLIYENGVLKQAITRGDGEKGDDVTNNVKTIRTIPLVLHGN
                     QYPAFFEVRGEIVMPWEVFDMLNKEREEQDEPLFANPRNATSGTIKLQNSSLVAKRNL
                     DAYFYAVLGNNLPANTHYDNLLRAREYGFKVSTDTKKCSSLQEVLEYIEYWDIARKQL
                     PVATDGIVIKVNSLTQQRFLGLTAKSPRWAIAYKFQAEKAVTRLNSVSYQVGRTGAVT
                     PVANLDPVLLSGTIVKRASLHNASIIASLDLYVGDSVFVEKGGEIIPKITGVDLDARI
                     LIGSKIQFITHCPECGTKLVNLPGEAAHYCPNEDDCPPQIKGKIEHFVSRKAMNIDGL
                     GKETIDLLYQQGLVKKVSDLYDLSAEQLSVLERLGEKSANNIIEAIEHTKQIPFERVL
                     FALGIRFVGETVAKKLAMAFHSIDKIQEASIQELVEVDEIGERIAQSVYEYLHNNKHQ
                     QLIQKLKDYGLQFTLAEDKLALKSTILAGKKFVISGTFSQFSRDELKDLIENNGGKNI
                     GSVSSQVDFIVAGDNMGPAKLAKAQKLNITLLTEQEFMAMIENGKTI"
     gene            4599..5273
                     /gene="trmD"
                     /locus_tag="PLD76_01905"
     CDS             4599..5273
                     /gene="trmD"
                     /locus_tag="PLD76_01905"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU26792.1"
                     /translation="MRIDIITVLPELIESNFSHSIIKRGIEKGLAEVHIHNLRDYSTN
                     KHKRVDDYPFGGEAGMVLQIEPIDKVITELKNQRTYDEIIFTSPDGETFNQKMANELS
                     LVNNIIILCGHYKGVDHRVREHLITKEISIGDYVLTGGELAASIISDAIIRIIPGVIS
                     DEQSALSDCFQDNLLSAPVYTRPAIYNGWKIPEILLSGHEKKISEWRMEQALNRTKKL
                     RPDLLK"
     gene            complement(5278..>6135)
                     /locus_tag="PLD76_01910"
     CDS             complement(5278..>6135)
                     /locus_tag="PLD76_01910"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOU26793.1"
                     /translation="QDIKPRQISPSKQKVLPVYWTRGFQVGLHLSQHYISPNWYRGGE
                     SHLALITLGSAFYNYNNKKNIQWENKLDFKAGFNSSTADSIRFLQTNEDVFRLTSKFG
                     YKAFTKFYYTGQTEFTTQLFNTYKVNTYDRITGAFSPIRLSVSGGLDYKESADFSIFF
                     SPVSYKYIYVNDTTKHISVAQSIADKLGIDKGKKSLHEVGSLVRINYRYKLSNEIEFD
                     TRTTIYTNYKGIEFDSEVNGNFKINRFLTTRLSVNPRYDSTFKLPNNDKARLQFRQIL
                     SFGFSYKFY"
BASE COUNT         2003 a         1124 c          876 g         2132 t
ORIGIN      
        1 acccaaaatt cttctgcttg gcgtaactgt gtagctacaa agtatccttt tgtttgcaac
       61 aatgtgatat aatatgtggc tatatctctt tgtttttcat tggtataaaa aattacagaa
      121 acgtattgcg acccaatatc aggtccttgc ccatcaattt gcgaaaaatt atgtgtttcg
      181 aagaataatt taacgagtgt ctcatattct acttgttcat tatcataaac aatttctact
      241 gtttctatgt gccctgtatc tcctgtacaa acctctctat aagttggagc aaatgtcagc
      301 ccacccatat atcctacttg agtcgcaatt cttggtagtt gcataaaata gtattcagta
      361 ccccaaaaac aaccagaagc aaaataggct ttttctatca ttattttttc atcaatttta
      421 ttgataatga atttacacaa tgtcttacat tcttttcagt aaaaccttca ccttcaaata
      481 catgtcctaa atgtccatga caatttgcac acacaatttc agttcttcta ccatccatat
      541 cgggaatttc caacacagct tcttttatag catcatcaaa acttggccaa ccacattccg
      601 aatcaaattt atcatcagac aaaaacaatg gcgtatcgca tctacgacaa ctatatatac
      661 catcttcttt ggtatgtaca tattctccgc taaaaggtaa ttcagtgcct ttatgcacta
      721 taatgtgttc ttcctcttta gttagtttgt tcatattatt aaaaaaaatg ctaatttttc
      781 aattagcatt taatgttcat atttaaaaga atgtttcgca ttttctcgta ggttgttatc
      841 cttgttggta ataaaggtaa tcgtaattgg ttttctaaat aacccattgc agatagaata
      901 ctttttactc cagcaggatt accatcgaca aacaatagtt caattaattc agtaaaccca
      961 tgatgaatgg ttaaagctgt ctgataatca ccagctaagg ctaagcgaac cattttacta
     1021 aattcgcaag gaaaagcatt accaataacc gaaataacac ctgaagcacc taaggttatc
     1081 aacggaaatg tgatcgcatc atcaccagag ataactaaaa aatctttagg tttattttta
     1141 ataatcgcat ctacttgagc tatgtttcct gatgcttctt tggtggcaat gatatttgaa
     1201 aaatcatttg ccaaacgcaa cgtagtttcg acactcatat taacacccgt acgacctgga
     1261 acattataca atatgatagg caattttgta cttttggcta ctgctgcata gtgttggtaa
     1321 ataccttctt gtgtaggttt gttataataa ggagttactg ataaaattgc atcaacacca
     1381 tttaaatttg ctgttgaaat tttttgtacc aacgcacgtg tatcatttcc gcccataccc
     1441 aatacgatag gtaactttcc atttactttt tccacaataa actgagttat ttctaactgt
     1501 tcttcagcag ataaggtagg agtttcagct gttgtaccta acacaactaa ataatcagtt
     1561 ccatttttta gctgataatc aactaatcga gctaaagctg aaaaatcaac actgccatcc
     1621 gttttaaaag gtgttaccaa agcaacaccc attcctataa atttattgcg ttccattaaa
     1681 aagtgtattt tttctttaag ttgcaaaaat agtatttttt attcaaaaac agctactata
     1741 agaaaggatt tttgggaatt ttgtacaatg tataatcaat tattatacat tacgatttaa
     1801 tctgttttaa atagaaagca attgtttcta aaagtttatc ctcattaacc tgttcctctt
     1861 tcatttcaat cattaaatcg tataaaaatt tattttcttt tttcattgca accttacaac
     1921 caacctgtga gttagctact atatattgta aaggaatact atccttaagt gatatatcta
     1981 ttaatatatc tgcagaaata ttcaacgcat caagcataat attatcgtta ggaacacaaa
     2041 gccatgaaca atcattttgg gtaaagagta acatattgat gcgtttctct tctgcaagta
     2101 cccctttttt tacaaacagt acagaaaaca ctgtttttcc cattgctgta aaatctttaa
     2161 tgtttttgta tactatttca tatttactat aatcatctat ataatagagt aatgcaacag
     2221 aagatatgtt gtcgaatgaa aggtattttt tatctctcca ttgtttttta tgaaatttga
     2281 gtaaaaaata tcttcgtaat ttgaaaagaa tgttttgtaa ttgattcatc ttttttagta
     2341 taatttttgc cttattaaat aatgctattt cttttaaata gttttgccat tttcgatcat
     2401 tgccataaac tcttgttccg ttagtaatgt aatgtttaat ttttgtgctt tagccaactt
     2461 agcaggaccc atattgtctc cagccacaat aaaatcaacc tgagaggata cagacccaat
     2521 atttttacct ccattatttt caatcaaatc ttttagctca tcacgcgaaa actgagagaa
     2581 agtgcctgat ataacaaatt ttttaccagc taatatagtt gattttagag ctaatttatc
     2641 ctcagcaagt gtaaactgta aaccataatc ttttaatttt tgtatcaatt gttgatgttt
     2701 gttattgtgt aaatactcat atacactttg agcaatcctt tctcctattt catcaacttc
     2761 aactaactcc tgtatactag cttcttgtat cttatcaata gaatgaaatg ccatagctag
     2821 ttttttagca accgtttcac ctacaaaacg aatgccaagg gcaaataata cacgttcaaa
     2881 aggaatttgt tttgtatgtt caatagcttc tattatattg ttggcagatt tttcgcctaa
     2941 tcgctctaat acagacaatt gttctgctga taaatcatat aaatctgata ccttttttac
     3001 taaaccttgt tgatacaata aatcaatggt ttcttttccc aaaccatcaa tattcatagc
     3061 tttccggcta acaaaatgtt caatttttcc cttaatttgt ggtggacaat catcttcatt
     3121 tgggcaataa tgtgcagcct ccccaggtaa attcactaac ttagtgccac actctggaca
     3181 atgagtaata aattgaatct tactgcctat caaaattcgt gcatctaaat ctactcctgt
     3241 tattttaggg attatctccc ctcctttttc tacaaatacg ctatcaccta catataaatc
     3301 taaagaggca ataatacttg cattgtgtag cgacgctctt ttaacaatag ttcctgacaa
     3361 taacactgga tctaaattgg caacaggtgt tacagctcca gttctaccta cctgatatga
     3421 cacagaattg agcctagtaa ctgccttttc tgcctgaaac ttataagcaa ttgcccatcg
     3481 aggggatttg gcagttaatc ccaagaaacg ttgctgtgtc aatgaattga ctttaataac
     3541 aattccatca gttgccacag ggagttgttt acgagcaata tcccaatact ctatatactc
     3601 taaaacttct tgtaaggaac tgcacttttt agtgtctgta gatactttaa acccatactc
     3661 acgagctctt aataaattat cataatgagt attagcaggc aagttatttc ccaaaacagc
     3721 ataaaaataa gcatctaaat ttcttttggc taccagggat gaattttgca atttaattgt
     3781 tccagaggtt gcattacgcg gatttgcaaa caaaggttcg tcttgctcct ctcgttcttt
     3841 atttagcata tcaaatactt cccaaggcat tacaatctct cctcttactt caaaaaaagc
     3901 tggatactga ttgccgtgca acacgagtgg aattgtacgt attgttttca cattgttagt
     3961 tacgtcatct cccttttcac catctcctct tgtaatggct tgttttaaca ctccattttc
     4021 gtaaatcaaa gagatagatg tcccatcaaa tttcaattca cagactaact caaatggctc
     4081 atcaatagat tttgataccc tttcgtaaaa atcacgtact tctccttctg aatatgtatt
     4141 tgataatgat aacattggat attgatgtga aacttgtaca aaactagttg tactatcact
     4201 tcctacccgc tgcgtaggag aattactatc atagtattct agatgcaatt cttctaatgc
     4261 ctgtagtttt ttcagtaaaa aatcaaattc aatatcggaa attgtaggct gagaaagcac
     4321 ataatactca taattatatt gatgtaactg tttacgcaag tgcaaaattt tttgatgtat
     4381 ttcgttcatt atcaaataat tatttaacta taaagtaata agaaactatt tgtttctagt
     4441 atgattcaat agaaatgcta ttcagtatta caactcaatt gagcaaacaa atgtaataaa
     4501 aatcgtgttt tgatttggta ttttatgtat atacttttca acaatccaca agtatgaaaa
     4561 tttttacgta cttttgtcga aaatccattt gtagttatat gcgtatagac atcattacag
     4621 tattacctga attaattgaa agcaatttct ctcattcaat tatcaaaaga ggaatagaaa
     4681 aaggtttggc agaagtacac atccataatt tacgtgatta ctctaccaat aagcataagc
     4741 gagtagatga ttatccgttt ggtggcgaag ctggcatggt tttgcaaatc gagcctattg
     4801 acaaggtaat aactgaacta aaaaatcaac gtacatacga tgaaataatt tttacttctc
     4861 cagatgggga aacttttaac caaaaaatgg cgaacgaact ttcacttgtt aataatatca
     4921 ttatactttg tggtcattat aaaggagtgg atcatcgtgt acgagaacat ttaataacta
     4981 aagaaatttc tataggagat tatgttctaa caggaggcga attagctgca tcaataatat
     5041 cagatgctat cattcgcata atacctggag ttatttctga cgaacaatcg gcattgtctg
     5101 attgctttca agacaactta ctttctgccc ctgtttatac acgtcctgct atttataatg
     5161 gatggaaaat tcctgaaata ttgttatctg gacacgaaaa aaaaataagt gaatggcgta
     5221 tggagcaagc tctaaacaga acaaaaaagc tacgtccaga tttactaaag taacaactta
     5281 gtaaaattta tatgaaaatc caaaactcaa tatttgccta aactgtaaac gtgctttgtc
     5341 attatttggc aacttaaatg ttgaatcata ccgtggattt accgagaggc gtgttgttag
     5401 aaatctattt attttgaaat tgccatttac ttcggagtca aactctatac ctttatagtt
     5461 ggtatatatc gtagtacgtg tatcaaattc aatctcatta ctcaatttat acctataatt
     5521 tatacgcacc aaactaccta cttcatgtaa cgatttcttc cctttatcaa ttcctaattt
     5581 atctgctatt gattgagcaa cggaaatatg tttcgtagta tcattcacat aaatatattt
     5641 ataagaaaca ggtgaaaaaa atatggaaaa atcggcactc tctttataat ctaaaccacc
     5701 acttactgat aatctgatag gagaaaaagc acctgtgata cgatcatatg tattaacctt
     5761 ataggtgtta aagagttgtg tcgtaaattc tgtttgacca gtataataaa atttagtaaa
     5821 tgctttataa ccaaatttac tagtcaaacg aaacacatct tcatttgtct gaagaaatcg
     5881 aatagaatca gcagttgagc tattaaaccc agccttaaaa tcaagcttat tttcccattg
     5941 tatgtttttc ttattattgt aattataaaa agcactaccc agtgtaatca aagctaaatg
     6001 actttcccct cctctatacc aattaggaga tatgtaatgt tgtgaaaggt gtaaccctac
     6061 ctgaaaacct ctcgtccaat atactggtaa caccttttgc ttgctcggag aaatttgacg
     6121 gggtttaatg tcctg
//
LOCUS       DAOSMY010000047         6124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_12955_length_6124_cov_4.070358, whole genome shotgun sequence.
ACCESSION   DAOSMY010000047 DAOSMY010000000
VERSION     DAOSMY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12955_length_6124_cov_4.070358"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..730
                     /locus_tag="PLD76_01915"
     CDS             212..730
                     /locus_tag="PLD76_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOU26794.1"
                     /translation="MKTVTITEQPKIDEVIRSCQFCFVAVADIDATPYVFPMNFGYQD
                     GILYLHSGPQGRKTDILEKNPKICISFCANASLYYTAEQIACTYLMQTKSVIIDGQVE
                     FIADDAEKIRIMNIIMAHYTAKTDFQYSDPAIKNVRVWKVQPTQVTCRFLGVPHREAM
                     QLEKTQHIKKNV"
     gene            748..1323
                     /locus_tag="PLD76_01920"
     CDS             748..1323
                     /locus_tag="PLD76_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOU26795.1"
                     /translation="MKSIKGTKTEQNLLISFAGESQARNRYTFFASQAKKEGFEQIAA
                     IFTETAEQEKEHAKRFFKFLEGGMVEITASYPAGVIGTTAENLLAAAQGEHEEWSKDY
                     PLFADIADEEGFPEVAQTFRAVALVEEMHENRYLAFWKNVKEGKVFVKDEEVVWQCRN
                     CGYIVSSNEAPDECPACKHPQSYFEVMKTNI"
     gene            1387..2031
                     /gene="mnmD"
                     /locus_tag="PLD76_01925"
     CDS             1387..2031
                     /gene="mnmD"
                     /locus_tag="PLD76_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOU26796.1"
                     /translation="MQIITTSDGSHSVYSPQFDEAYHSSFGAMQEAEYIFINKGLLYT
                     AKTSLQVLEIGFGTGLNAFLTSVYGCNLSIYYTGIERFPLSVEEANLLNYASFFPEQK
                     AWFSAIHQVEWGSFQQVNDNFQLCKVQADVLHIDFAKEQYDVVYFDAFSPEKQPEMWT
                     ESVFTRIFEACKPGAVFTTYCCKGYVRRLLQGVGFTVERLPGPIGKRQILRAIK"
     gene            2035..2709
                     /locus_tag="PLD76_01930"
     CDS             2035..2709
                     /locus_tag="PLD76_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU26797.1"
                     /translation="MKTKTYFQQLVTSLKVNKFYLLPYLIVWLMGLLVVLLYDKIDIH
                     QFTNQRPCGIGDALFPYITKLGETFPFIVGGALLLYKVRKALFVLSVQVVGAIVVYTL
                     KNLFRAPRPRIVFQELGLDLHTIDGVRLHAWNSFPSGHTMAAFAFFLSLALIVKNQLL
                     KFFFFAMSLLVGFSRIYLNQHFLEDTLAGSLLAVVIALTMYPFFNTQQTWGNKGLMSL
                     ANKSTK"
     gene            complement(2698..3630)
                     /locus_tag="PLD76_01935"
     CDS             complement(2698..3630)
                     /locus_tag="PLD76_01935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOU26798.1"
                     /translation="MPKVVGIGETVYDIIFENNRPIDGKPGGSVFNALISLGRLQQQT
                     AFISEVGDDTVGKIILDFLAQNKVNADYVAQFDGTKTALSLAFLDENKNANYTFYKDY
                     SAQKAPHKTPPITSKDIVLFGSFFSLNSTVRQQVTTLLDTANKESAMVYYDVNFRKTH
                     ISKRHELLPALIENMKYADIVKGSEEDFDHLYDEKDPITIYQKYIAPHCSTFIYTQGA
                     NGAMVFTPTYSLHVPSIPIVPISTIGAGDTFNAGIIFALLQQNIGKDTLCNVQQHEWE
                     AILQQAIDCATHVCMLYDNYITVDFANKKLDLFR"
     gene            complement(3632..4621)
                     /gene="trpS"
                     /locus_tag="PLD76_01940"
     CDS             complement(3632..4621)
                     /gene="trpS"
                     /locus_tag="PLD76_01940"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU26799.1"
                     /translation="METVLSGIRSTGNLHLGNYYGALRNFIKMQEEYQCFFFIADYHS
                     LTTHPNPHEMHGNVRNVLTEYLAAGIDPDKATIYVQSDVPEIPELYLLLNMHAYLGEL
                     SKTASFKEKARKQPENVNAGLLTYPTLMAADILIHNAHKVPVGKDQEQHLEMTRKFAR
                     RFNNFYQVEYFREPAAFNFGEDLLKIPGLDGSGKMGKSEGNCVFLADDAKTIEKKVKR
                     ALTDAGPTAPNSTKPDYIENLFTILKVVSTPEVVTHFEEKWNTCDIRYGDLKKQLAED
                     IIRATSPIRERIIDIQNNEAYLAKVTKEGAEKARISAAKTLREVREIMGFKKF"
     gene            complement(4649..>6124)
                     /locus_tag="PLD76_01945"
     CDS             complement(4649..>6124)
                     /locus_tag="PLD76_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOU26800.1"
                     /translation="VNVSQGIKFFLVGLPDNAVKESHERIVSALQYNGHKFPRQQIII
                     NMAPADIRKEGSAYDLPLAMGIMAAAEQIACEKINDYLIMGELSLDGNIMPIKGALPI
                     AIKAREAGFKGLILPKQNAKEAAVVNNLEVLGVENIQEVIGFLNNDTTIEPTIVHTRD
                     EFYSMASDFEMDFSDVKGQETVKRAMEVAAAGGHNIIMIGPPGAGKSMLAKRIPSILP
                     PLTLHEALETTKIHSVAGKIEKGISLMYKRPFRSPHHTISDVALVGGGSFPQPGEISL
                     AHNGVLFLDELPEFKRTVLEVMRQPLEDRKICISRSKFSIEYPASFMLVSSMNPCPCG
                     YYNHPEKDCVCGPGIVQKYLNRISGPLLDRIDIHIEIVPVPFEKLSCAKPTEHSEVIR
                     ERVIKARKIQEERFKENDGVHCNAQMSSKLLREHTKLSDTSQQLLKVAMQRLNLSARA
                     YDRILKVARTIADLDDAKDIQVEHISEAINYRNLDREGWAG"
BASE COUNT         1765 a         1131 c         1202 g         2026 t
ORIGIN      
        1 ggtgtacaag tttaaacgtc tttgttgagc cccactcata caatctaaag gcatgcaata
       61 attcctacta acaatcattt ttttttcatc accaacaaac ttgcttttct gtgtgttaaa
      121 aatgcatcaa gtagttgtaa gtttagtaat atatctgtat cttcgcaggt gtttctgaaa
      181 tgttaataaa aacaaaaaag tagctcagta gatgaaaaca gtaacgataa ccgaacaacc
      241 aaaaattgac gaagtcattc gttcatgcca attttgtttt gtggcagttg ccgatataga
      301 tgctacgccc tacgtatttc cgatgaattt tggctatcaa gatgggattt tgtacttgca
      361 ttctggtcca caaggaagaa agacggatat attagaaaaa aacccgaaaa tttgtattag
      421 tttttgtgcc aatgcgtcgt tgtattatac tgccgagcaa atagcttgca cctatttaat
      481 gcaaaccaaa tccgtaatta tcgatgggca agttgagttt attgctgatg acgcagagaa
      541 aatacgtatc atgaacatta tcatggcaca ctatacagcc aaaacagatt ttcaatattc
      601 agatccagcg attaaaaatg tacgcgtgtg gaaagtgcaa cctacgcaag taacgtgccg
      661 ctttttgggc gtgccacaca gagaagccat gcaattagaa aaaacacagc atattaaaaa
      721 gaacgtttaa cacccaaaaa aattaccatg aaatcaatta aaggaacaaa aacagaacaa
      781 aacctgctta tctcttttgc aggagaaagc caagctagaa atcgttatac tttttttgct
      841 agtcaagcca aaaaagaagg atttgaacaa atagctgcaa tttttactga aacagcagaa
      901 caagaaaaag aacacgctaa acgttttttt aaattcttag aaggcggtat ggtagaaatt
      961 actgcatcgt atccagctgg tgttattggt acaactgccg aaaacttatt ggcagcagca
     1021 caaggggagc acgaagagtg gtcgaaagat tacccactat ttgctgatat tgctgacgaa
     1081 gaaggtttcc ctgaagtagc acaaactttc cgtgcagtag cattggtaga agaaatgcac
     1141 gaaaatcgtt accttgcatt ttggaaaaat gtaaaagaag gaaaagtatt tgttaaagac
     1201 gaagaagtag tatggcaatg tcgtaattgt ggttatatcg ttagctctaa cgaagcacca
     1261 gacgagtgtc cagcttgcaa acatccacaa tcgtatttcg aagtgatgaa aacaaatatc
     1321 taaataaaat atcttagaac attcaatcgc tacattattt tgtggcgatt gttgtttttt
     1381 tactatatgc aaattattac tacttcagac ggttcgcatt cggtatattc gccacaattt
     1441 gatgaggcgt atcattcgtc gtttggtgcc atgcaagagg ctgaatacat ctttattaat
     1501 aaaggcttac tgtacactgc taaaacatcc ttgcaggtac ttgaaattgg tttcggtacg
     1561 ggtctaaatg cttttttaac aagtgtttat ggctgcaatt tatccatata ttatacaggt
     1621 attgaacgtt ttccgctatc agtagaggaa gcaaacctat tgaattatgc aagtttcttt
     1681 cctgaacaaa aggcttggtt ttctgccatt catcaagtag agtggggtag ctttcaacag
     1741 gttaatgata attttcagtt gtgtaaagtg caagcagatg tgttgcatat tgattttgcc
     1801 aaagaacaat atgatgtagt gtattttgat gccttttcgc cagaaaaaca acccgagatg
     1861 tggacagaat cagtttttac acgtatattt gaggcatgca aaccgggtgc agttttcaca
     1921 acttattgtt gtaaaggcta tgtacgtagg ttgttgcaag gggtcgggtt tacagtagag
     1981 cgtttaccag gaccaatagg aaagcgtcaa atactgagag caataaagtg aaatatgaaa
     2041 acaaaaactt attttcagca acttgttact tcgctaaaag tcaataaatt ttacttattg
     2101 ccgtatctta ttgtatggtt aatgggtttg ttggtagtgt tgctgtatga taaaattgat
     2161 atacaccaat ttaccaatca acgtccgtgt ggcataggtg atgccttgtt tccgtatatt
     2221 actaaattag gagaaacctt tccgtttatt gttggaggtg cattgttatt gtataaagta
     2281 cgtaaggctc tttttgtatt atcggttcag gtagttggtg caattgtggt atatacgcta
     2341 aaaaatctgt ttagggctcc ccgtcctcgc atagtttttc aagagttagg cttggattta
     2401 catacgattg atggcgttcg tttgcatgcg tggaacagtt tcccgtctgg tcatacaatg
     2461 gcagcatttg cttttttctt atcacttgcc ttgattgtaa aaaatcagct actcaaattc
     2521 ttcttttttg ccatgtcgtt gttggtcggt ttttcacgta tttatttgaa ccagcatttt
     2581 ttagaagata ccttagcagg atccttactt gccgttgtga ttgcattgac gatgtaccca
     2641 ttttttaata cccaacaaac atggggaaac aaagggctaa tgtctttagc aaataaatca
     2701 acgaaataaa tccagttttt tattggcaaa atctacggtg atgtaattat cgtagagcat
     2761 acacacatga gtagcacaat caatcgcttg ttgtaggatg gcttcccatt cgtgctgttg
     2821 gacattgcat aaagtatctt ttccaatgtt ctgttgcaac aaggcaaata ttattccagc
     2881 attaaaagta tctccagcac cgatagtact tattggaaca atgggtatag aaggtacgtg
     2941 tagcgaatag gtgggtgtaa ataccattgc accattagct ccttgtgtat aaataaacgt
     3001 tgaacaatgg ggagcaatgt atttttggta aatcgttatt gggtcttttt catcgtaaag
     3061 atgatcaaaa tcctcctcag aaccttttac aatatcggca tacttcatat tttcaatcaa
     3121 agcagggagt aattcgtggc gtttgctgat gtgtgttttt ctaaaattta catcgtaata
     3181 caccatcgca ctctctttat tagcggtatc aagtaatgtt gtaacttgtt gcctgacagt
     3241 gctatttaag gaaaagaatg agccaaacaa tacaatatcc ttgcttgtaa tgggcggtgt
     3301 tttatgtgga gctttttgag cagaataatc tttgtaaaag gtgtagtttg catttttgtt
     3361 ttcatctaaa aaagctaaag ataaagccgt tttagtgcca tcaaattgtg ctacataatc
     3421 cgcatttact ttgttttgag ccaagaaatc cagaattatt tttccaacag tatcatcgcc
     3481 tacttcactt ataaaagcgg tttgttgttg caatcgacca agcgaaataa gtgcattgaa
     3541 cacagaaccg cccggtttgc catctatcgg acgattgttt tcaaaaatta tatcatatac
     3601 agtttcgcct attccaacaa cttttggcat attaaaattt tttgaatccc attatttctc
     3661 ttacttcgcg taatgtttta gcggcactta tgcgtgcttt ttctgctcct tctttggtta
     3721 cttttgccag gtatgcttcg ttgttttgta tatcaatgat gcgttcgcga ataggagaag
     3781 tagcacgaat aatatcttct gctagttgtt tttttaggtc tccataacgg atatcgcaag
     3841 tgttccattt ctcttcaaaa tgtgttacta cttctggggt agaaactact tttaagatag
     3901 taaataggtt ttcaatataa tcaggtttgg ttgagttggg tgccgtcgga ccagcatctg
     3961 ttaaggctct ttttactttc ttttctattg ttttggcatc atcagccaaa aatacacagt
     4021 ttccttctga ttttcccatt tttccactac cgtctaaacc aggaatcttg agcaagtctt
     4081 ctccaaaatt aaaagcagct ggttctctaa aatattctac ttgatagaaa ttattaaacc
     4141 tgcgagcaaa tttgcgtgtc atttctagat gttgttcttg gtcttttcct acaggcactt
     4201 tgtgagcatt gtgtattaaa atatctgctg ccattaaggt tggatacgtt aataatccag
     4261 cattcacatt ttctggttgt ttgcgggctt tttccttaaa tgaggctgtt tttgagagtt
     4321 ctcccaaata ggcatgcatg ttgagtaaaa ggtataactc aggaatttca ggtacatcgc
     4381 tttgcacgta aatagtcgcc ttgtcggggt caatacccgc tgctaggtat tcagtcaaca
     4441 cattgcgtac gttgccatgc atttcgtgtg ggtttggatg cgtagtaagc gagtggtaat
     4501 cggctataaa aaagaaacac tggtattctt cttgcatttt tataaaattg cgtaaagcac
     4561 cataataatt tcctaaatgt aaatttccag ttgacctaat gccactaagt actgtttcca
     4621 tagtatataa ttttttttag ttttttattt aacctgccca accttcacgg tcgagattgc
     4681 gataattgat agcttcggat atatgttcta cttgaatatc ctttgcatca tctaaatcgg
     4741 ctattgttcg ggctactttt aatattctgt cgtaagctct tgctgaaaga ttgagccgtt
     4801 gcatggcaac cttcaacaat tgttgactag tatcgctgag tttggtgtgc tctcgtaata
     4861 attttgacga catttgtgca ttgcagtgta cgccatcatt ctctttaaaa cgttcctctt
     4921 gtatttttcg tgcctttatt acacgttcgc gtattacttc gctgtgttct gtaggtttgg
     4981 cacatgacaa cttttcgaaa ggaacaggca caatttcgat gtgtatgtca atgcggtcga
     5041 gcagtggtcc tgaaatgcgg ttgaggtatt tttgcacaat accaggtccg catacacaat
     5101 ctttctccgg gtgattatag tacccacatg ggcatggatt cattgatgat actaacataa
     5161 aactagctgg atactcaatg ctaaattttg agcgtgagat gcaaattttt ctatcttcga
     5221 gtggttgacg cattacctct aatacggttc gtttaaattc gggtagctcg tctaaaaaca
     5281 ataccccatt atgagccaac gaaatttcgc ctggttgtgg aaaactccct ccgccaacta
     5341 gtgctacatc agaaatagta tggtgcggac tgcgaaacgg acgtttgtac atcaacgata
     5401 tacctttttc aatttttcca gctaccgaat gtatttttgt tgtttctaat gcttcgtgta
     5461 aggtaagtgg tggcaagata gatggaatgc gttttgccaa catcgatttt ccagcacctg
     5521 gaggtccaat cataataata ttgtgaccac ctgcagctgc cacttccata gcccgtttta
     5581 cggtttcttg tccttttaca tccgaaaaat ccatttcaaa atcactagcc atagaataaa
     5641 actcatctct ggtatgaaca atggtcggct ctatcgtagt atcattattt aaaaacccaa
     5701 ttacttcttg aatattttct acacctagca cttcgaggtt gttcactaca gccgcttctt
     5761 tggcattttg tttgggcaga atcaatcctt taaatccagc ttctcgtgct ttaatggcaa
     5821 tgggcaaggc tcctttgatg ggcataatat tgccatcaag agataattct cccatgatca
     5881 aataatcgtt tattttctcg caggctattt gctcggctgc cgccataata cccatagcca
     5941 aaggtaaatc gtatgccgaa ccttctttgc ggatatcggc aggagccatg ttgatgatga
     6001 tttgttggcg agggaattta tgcccgttgt attgcaaggc ggatacaatc cgttcgtggc
     6061 tttcttttac ggcattatcg gggagtccta ctaaaaagaa ctttatacct tgcgatacgt
     6121 tcac
//
LOCUS       DAOSMY010000048         6048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_13209_length_6048_cov_3.768230, whole genome shotgun sequence.
ACCESSION   DAOSMY010000048 DAOSMY010000000
VERSION     DAOSMY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13209_length_6048_cov_3.768230"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLD76_01950"
     CDS             complement(<1..254)
                     /locus_tag="PLD76_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU26801.1"
                     /translation="MGKTVNIITLGCSKNTVDSERLARQLAAIGYSVSFDSNDFSEVV
                     IINTCGFIHDAKEESIEMILQAIQAKEAGEIEHVYVIGCLS"
     gene            complement(322..1317)
                     /locus_tag="PLD76_01955"
     CDS             complement(322..1317)
                     /locus_tag="PLD76_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOU26802.1"
                     /translation="MVSYKELGLVNTKQMFQKAVEGGYAVPAFNFNNLEQIQAIVSAC
                     AETKSPVILQVSSGARKYANQTLLRFLAQGAVEYAKELGWEKPQIALHLDHGDTFELC
                     KSCIDMGFSSVMIDGSHHSFEENIALTKKVVEYAHAHGVTVEGELGVLAGVEDDVVAE
                     HSTYTQPAEVIEFVQRTGVDSLAISIGTSHGANKFTPAQCTRNAEGLLVPPPLRFDIL
                     EEIEKQIPGFPIVLHGASSVPQEYVATINSNGGALKDTIGIPEEQLRRAAASAVCKIN
                     IDSDGRLAMTAAVRKFFNDNPDKFDPRQYLGPGREELKKLYTHKVVNVLGSAGKA"
     gene            complement(1462..2829)
                     /gene="radA"
                     /locus_tag="PLD76_01960"
     CDS             complement(1462..2829)
                     /gene="radA"
                     /locus_tag="PLD76_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU26803.1"
                     /translation="MPKLKTIYVCKNCGNDSPKWFGKCSACGEWNTCVEEIEHKESKS
                     APRITGYDFQAQKPILLHEIEISQEKRIDMCNEEVNRVLGGGLVPGSMVLIGGEPGIG
                     KSTLVLQVALHLSNLRILYVSGEESAKQLKLRAERIASHQTDCYIVSETSLETIYKHI
                     QTIKPELLIIDSIQTIFTDVVDSSPGSITQVRECAASLLKFAKESNTPIFIIGHITKD
                     GVIAGPKILEHIVDTVLQFEGDQHYMYRILRSIKNRFGSTSELGIFEMRQNGLREVTN
                     PSELLLSHHQEELSGIAIASAIEGVRPFLIEVQALTSTAAYGNPQRSATGFDGRRMNM
                     LLAVLEKRAGFKIAQKDVFLNIAGGLKVKDPAIDLSVISAILSSSMDIAIQTNCCLAG
                     EVGLSGEIRPVNRIEQRIAEAQKLGFTKIILPNHNMKGLTSTFPEIELVPVKKVEDAF
                     RILFT"
     gene            complement(2906..3256)
                     /locus_tag="PLD76_01965"
     CDS             complement(2906..3256)
                     /locus_tag="PLD76_01965"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOU26804.1"
                     /translation="MFIQVLKSKIHRVTITEANLEYIGSITIDEDLMEAANIIESEKV
                     HIVDNNNGERFETYVIKGERGSGTICVNGAAARRVQPGDTIIIMAYAFMDFEEAKQFK
                     PTCIYPDTRTNKLI"
     gene            complement(3263..4099)
                     /gene="panC"
                     /locus_tag="PLD76_01970"
     CDS             complement(3263..4099)
                     /gene="panC"
                     /locus_tag="PLD76_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU26805.1"
                     /translation="MQIITTKSELCKSLTNSTGIRIGFVPTMGALHQGHLSLVMRSKR
                     ENEVTVVSIFVNPTQFNDSRDLEKYPRTLDADINLLSQVGCDIVFAPTVDEMYPEKDT
                     RIFNFGTLETVMEGKHRPGHFNGVAQIVSKLFDAVQPHIAYFGEKDFQQLAIIREMVK
                     QLNYTITIAGCPIVRESSGLALSSRNMLLSAEEHKKAPIIYQILSESCIFVAQKQPHE
                     VRNWVLNKFQNTGLDVEYFEIVDGYSLQPVSSWNESDYIVGCIAVFCGKIRLIDNIPF
                     KR"
     gene            4366..5181
                     /locus_tag="PLD76_01975"
     CDS             4366..5181
                     /locus_tag="PLD76_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOU26806.1"
                     /translation="MDNVIKVLYVTQEICPYLPESEIADICRKLPQAIQEKGKEIRTF
                     MPKFGCINERRNQLHEVIRLSGMNLIIDDTDHQLIIKVASIQAARMQVYFIDNDDYFN
                     RKSTTQDENGEDFDDNEDRAIFFARGVIETVKKLRWTPDIVHCHGWMTALMPIYIKKA
                     LHDDPFFSKAKVIYSIYDNGFSKHFSPQFAQKSILEGIEKTDLKSVQNDGSFDAVTRL
                     AIDFSDGVIAASENVNTELLEYAKKSQKMTLPYQSPENYVNSYNEFYDQIVGQ"
     gene            5195..>6048
                     /locus_tag="PLD76_01980"
     CDS             5195..>6048
                     /locus_tag="PLD76_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOU26807.1"
                     /translation="MKNTFWLLIAISFLFASCKDESEDLGFLIQPSKDKITVVIDSFH
                     VATSNLFVDSIYARSSNFTLGKYTDNIYGSFNASFLTQFKYVQGATFPSNATADSLSL
                     VLYYKSFWGDSLSTHEVTVYALDELFNYEKNYYSNINVAEFTNKQTILGKKVYTAVDK
                     TIPDSIRNASDYVGSVTILLPNSLKEEFFTRSDIYTSQQAFLNFFKGIYATTTYGDGT
                     VLLIDSANIELKYSYATTTSADVDTIIKEKLIFPANKEITSVTHIDHPTALSTSITDI
                     DSIVYVHSP"
BASE COUNT         2000 a         1139 c          916 g         1993 t
ORIGIN      
        1 gaaaggcaac caataacata cacatgttct atctctcctg cttcttttgc ctgtatagct
       61 tgcaaaatca tttcaattga ttcttcttta gcatcgtgaa taaacccaca cgtgttaata
      121 ataaccactt cagaaaaatc attggaatca aacgaaaccg aatagcctat tgcggcaagt
      181 tgccgagcaa gacgctcgct gtctaccgta tttttggagc agcctaaagt tataatattt
      241 actgttttac ccatatttac aatcaaaaaa aaaggtcgct atattgcaca gcgaccttta
      301 ctatattact aacgcaatag attatgcttt accagcagaa cctaaaacat ttactacttt
      361 atgagtatac aattttttca actcttcgcg accaggacct aaatattgac gagggtcaaa
      421 tttatcagga ttatcgttga agaatttacg tacagcagca gtcatagcca aacgaccatc
      481 agagtcgata ttgattttac atactgcaga tgcagcagca cgacgtaatt gttcttcagg
      541 aattccaata gtatctttta atgcacctcc gtttgaatta attgtagcaa catattcttg
      601 aggaacagat gaagcaccat gtaatacgat agggaaacca ggaatttgtt tttcgatttc
      661 ttctaaaata tcgaaacgta atggaggagg aactaataaa ccttcagcat tgcgagtaca
      721 ttgagctggt gtaaatttgt tagcaccatg tgatgttcct atagaaatag ccaatgaatc
      781 tacaccggta cgttgaacga attctataac ctctgccggt tgggtataag tagaatgttc
      841 tgcaactacg tcatcttcaa cacctgccaa tacacctagt tcaccttcta cagttacacc
      901 atgtgcatgt gcatattcta caactttttt agtcaacgct atattttctt cgaatgaatg
      961 gtgagaacca tcaatcataa ctgaagaaaa tcccatatcg atacagcttt tacataattc
     1021 gaatgtatcg ccatgatcta agtgaagagc tatttgtggt ttttcccaac ccaattcttt
     1081 tgcgtattct accgcacctt gagccaagaa acgtaacaat gtttgatttg catatttacg
     1141 agcaccactt gatacttgta aaataacagg cgatttagtt tcagcacaag ccgatacaat
     1201 agcttgaatt tgctctaaat tattaaaatt gaatgcagga acagcatagc ccccctccac
     1261 tgctttttgg aacatttgtt tggtgttcac taaaccaagt tctttgtaag aaaccatttt
     1321 tttttgtgtt aagtttatga atcgtttaat tgaattatat tctttttgca tggcaaaatt
     1381 aaaaaaaata atctacatac ttgcctaatt ctcaaaaaaa atacacatat gctttcctta
     1441 aaaaaacaaa attaaccatc cttaggtaaa taaaatacga aaggcatctt ctactttttt
     1501 cacaggcaca agttctattt ctggaaaggt tgatgtcaag cctttcatat tatgattggg
     1561 aagtataatt ttggtgaatc ctaatttttg agcctcagca atgcgttgct caattctatt
     1621 cacaggtcta atttcacccg ataagcctac ctctcccgcc aaacaacaat ttgtttgaat
     1681 agcgatatcc atacttgaag ataaaatagc actaattaca gacaaatcaa tggcaggatc
     1741 ttttactttt aatccaccag caatattgag aaagacatct ttttgtgcaa ttttaaaccc
     1801 ggctctcttt tccaatacag ccaacaacat attcatacgt ctaccatcaa atccagtagc
     1861 tgaacgttgt ggattgccat acgcagcggt actagttagt gcttgcacct ctattagaaa
     1921 tggacgaaca ccttcaatgg cagatgcaat agcaatacca ctcaattctt cttgatgatg
     1981 ggacagtaaa agttcagaag gatttgttac ttcacgcaaa ccattctgtc tcatttcaaa
     2041 tattcccaat tcagaggtac tcccaaaacg atttttaatt gaccgcaata tcctatacat
     2101 ataatgctga tctccttcaa attgtaatac tgtatctaca atatgttcta atattttagg
     2161 accagcaatt accccatcct ttgtaatatg tcctattata aaaatgggag tattcgattc
     2221 ttttgcaaat ttgagtaacg atgcagcaca ttctcttact tgtgtaatac tacctggaga
     2281 agaatcaaca acatcagtaa aaatggtttg aatagaatca ataatcaaga gttctggttt
     2341 gattgtttga atatgcttat aaatagtttc aagagatgtt tcacttacta tatagcagtc
     2401 tgtttgatgt gatgcaatac gttctgctct caattttaac tgttttgcac tttcctcccc
     2461 agatacatat aatatacgta gatttgacaa atggagagca acttgcaata ccaaggttga
     2521 ttttcctatc ccaggttcac ctcctattaa taccattgaa cctggcacta atcctcctcc
     2581 taataccctg ttgacttcct cattacacat atctatgcgt ttttcttgtg aaatttctat
     2641 ttcgtgtaat aaaataggct tttgagcctg aaagtcatat cctgttatgc gaggggctga
     2701 tttactctct ttgtgttcta tttcttccac acaagtattc cattctccac atgcagaaca
     2761 cttaccaaac cactttggtg agtcgtttcc acagttttta caaacatata tagtttttag
     2821 tttaggcata catacaaatt tgttgcaaga tacaagaaaa aatacaaata aaaaagccac
     2881 tctatttaaa aagtggcttt ttgtttcaaa ttaatttatt tgtacgagtg tctggataaa
     2941 tacaagtagg tttaaactgc ttagcttctt caaaatccat aaaggcatat gccatgataa
     3001 tgattgtatc gccaggctgt actcgacgag cagcagctcc atttacacaa attgtaccac
     3061 tacctctttc gccctttatt acatatgttt caaagcgttc tccattgtta ttatccacaa
     3121 tatgaacttt ttctgactca ataatattag cagcttccat caaatcttca tcaatagtta
     3181 tactaccaat gtattcaaga tttgcctcgg taattgttac ccgatgtatt ttagatttta
     3241 aaacctgtat aaacatactt ttttaacgtt taaatggtat attatctatt agtcttattt
     3301 ttccacaaaa tactgcaata caaccaacga tataatcgga ttcattccac gatgaaacag
     3361 gttgtaagga gtaaccatct actatttcaa aatactcaac atctaatcct gtattctgaa
     3421 acttatttag tacccaattg cgaacctcgt gaggttgctt ttgggctaca aagatacaac
     3481 tttctgataa tatttggtat attataggtg cttttttgtg ttcttcagca gaaagtagca
     3541 tatttcgact actcaaagcc aaaccacttg attctctaac aatagggcac ccagcaatcg
     3601 taatagtata atttaactgt tttaccatct cacgtataat tgctaattgc tgaaaatcct
     3661 tttcaccaaa ataagcaata tgaggttgta ctgcatcaaa taatttactt acaatctgtg
     3721 ccactccatt aaaatgtcca ggcctgtgct ttccctccat aaccgtttct aatgtaccaa
     3781 aattaaagat gcgagtatct ttttctggat acatttcatc aactgtaggg gcaaacacta
     3841 tatcacaccc tacttgagat aatagattaa tatcagcatc taacgtacgc ggatactttt
     3901 ctaagtctct gctatcatta aactgagtag gattaacaaa aatacttact acagttacct
     3961 cattttctct tttacttcgc attaccaacg acaaatgtcc ctgatgtagt gctcccattg
     4021 taggaacaaa accaatacga atgcctgtag aattggttaa tgatttacac aattcagatt
     4081 tggtagttat aatttgcatc atttacatca aaattgagat gcaaaagaac aaataatatt
     4141 tgggatgaaa aagaaaaacc cacataagtt atcatcaata tataaaaaga aactttttta
     4201 taccacaatc acattctgtt tgctatgata atatgattgt ttccaatcta aaaataatac
     4261 ttacaaaata gaatggattt atgggaaatt tcttgcttat taacgatttt ttttttatat
     4321 ctttgcatat tgtttttttc gaggagtatt actaattcat ttttaatgga caacgttatt
     4381 aaagttttat acgttacgca ggaaatttgc ccgtatttac cagaatcaga gattgcagat
     4441 atttgtcgta agttgcccca agcaattcag gaaaaaggca aagaaattcg cacatttatg
     4501 cccaaatttg gttgtattaa tgaaagacgc aaccaactac atgaggtaat tcgactatca
     4561 ggaatgaacc ttattattga tgacactgat catcaactca ttatcaaagt tgcctctata
     4621 caggcagcac gtatgcaagt ttattttatt gacaatgatg attattttaa cagaaaaagt
     4681 acgactcaag acgaaaacgg tgaagatttc gacgataatg aggatcgggc tattttcttt
     4741 gcaagaggtg taattgaaac agtaaagaaa ttgcgttgga ctccagacat cgttcattgc
     4801 catggatgga tgaccgcatt aatgcctatt tacattaaga aagcattgca tgacgatcca
     4861 ttcttttcta aagctaaagt catttattct atttacgaca atggtttttc caaacatttt
     4921 agtccacaat ttgcacaaaa atctatatta gaggggatag aaaaaacaga tttaaaatcc
     4981 gtacagaatg atggaagttt tgacgctgta actcgtcttg caatagattt ttctgatggt
     5041 gtaattgctg caagtgaaaa tgtgaataca gaattgcttg aatatgctaa aaaatcacaa
     5101 aaaatgacac ttccatatca atctccagaa aattacgtaa attcgtacaa tgaattttac
     5161 gaccaaattg tgggacaata aattttctca aaaaatgaaa aatacattct ggctactaat
     5221 agctatctct tttttatttg cttcatgcaa agacgaatca gaagatttgg gttttttaat
     5281 ccaaccttcg aaagacaaaa taacagttgt aatagattct tttcatgtag caactagtaa
     5341 cttatttgtt gactcaatat atgccagaag cagtaatttt acgttaggaa aatacactga
     5401 taacatatat ggcagtttta acgcttcttt tttaacacaa tttaaatatg tgcaaggagc
     5461 tacattccca tctaatgcaa cagccgactc tttaagcttg gtactatatt ataaaagttt
     5521 ttggggcgat tccctttcta ctcacgaagt aactgtatac gcattggatg aattgtttaa
     5581 ttatgaaaaa aattattatt ctaatattaa tgtagctgag tttaccaata aacaaactat
     5641 tcttgggaaa aaagtatata ccgctgtaga taaaacaatt ccagactcca ttcgtaatgc
     5701 aagtgattat gtaggaagtg taactatttt actaccaaat tctttaaaag aggagttttt
     5761 tacaagaagt gacatatata caagccaaca agctttttta aattttttca agggaatata
     5821 tgcaactaca acttatggag atggtactgt attactcatc gattcggcta atattgaact
     5881 aaaatatagt tatgcaacta ccacttctgc tgatgttgac actattatta aagaaaaact
     5941 tatttttcca gccaataagg agattacaag tgttacacac atagatcatc ctaccgcctt
     6001 atctacttca ataacagata ttgatagtat tgtatatgta cattctcc
//
LOCUS       DAOSMY010000049         6036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_13248_length_6036_cov_3.212841, whole genome shotgun sequence.
ACCESSION   DAOSMY010000049 DAOSMY010000000
VERSION     DAOSMY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13248_length_6036_cov_3.212841"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="ftsZ"
                     /locus_tag="PLD76_01985"
     CDS             complement(<1..931)
                     /gene="ftsZ"
                     /locus_tag="PLD76_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU26808.1"
                     /translation="MVNFDFSTNADTIIKVIGVGGGGGNAVNHMFNQGIKDVTFAVCN
                     TDYQDLRRSPIPIKIQLGDKGLGAGNIPEVAREAALETMDKIEELLDDSTKMVFITAG
                     MGGGTGTGAAPVIAEIAKNKSILTVGIVTIPFEFEGEKKIQKALNGVREMSKHVDALL
                     VINNERLIDIFPDLKMSTAFAKADNILTDAAKGIAEIITIPGYINVDFADVQTILKGG
                     GFAIMNTGFASGEKRVSKAIHDALNSPLLNNNDIKGAKRILLHVYTSKENELDMTETI
                     EIKHFLNNIAKDAEVIWGATYDENLEESIKITII"
     gene            complement(956..2263)
                     /gene="ftsA"
                     /locus_tag="PLD76_01990"
     CDS             complement(956..2263)
                     /gene="ftsA"
                     /locus_tag="PLD76_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU26809.1"
                     /translation="MNTSTPYILAFDLGTTKIIGAVAQKTTEGKLSIVAYEEEETKPT
                     TIKQGAIFNVADTAFIINSIVTKLRNKTKLNLTKAYIGIGGRYLRCKENTTKRTLGYN
                     EIVEQNLLDKLYQENFKLQSTEFDILDVITQEYLVDDEWVADPAGIACTQLEGRYKII
                     YTKSEINTGINKVKELLAEKSQLEIISTFPSPIATANGLISDDEKELGCMIIDFGGST
                     TSYSVFTKNYLREIGVIPLGAKTITNDIRELNLTEPQAEKLKQKLGNAMATLEVPDKR
                     IIIAKAAHEQEEKSIQSTFLAQIIEARASEILNFIITPLKQKGYIKQLGAGIIITGGG
                     AQLKNMRALVQSLSGVDVRTTVGVHLLTEDSISDDFKKPSYSQIIGLLSMGTENCVSE
                     TIEEKFIPSKKTKKTGKNLFVRFADSASQRLTDLFTDNSDTIQ"
     gene            complement(2274..3005)
                     /locus_tag="PLD76_01995"
     CDS             complement(2274..3005)
                     /locus_tag="PLD76_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26810.1"
                     /translation="MIKKIALIVVSVVFLSYLIYAAISFTHTPQEQVCSTIHIFKIDS
                     VDYNFIKRKEIEKQLTDNNIYPIGRKIDFAFTHAIEEKIKEMNYVKRVECYRTNHGDV
                     VIRIWQRQPIVRIITPFYSYYIDQDRKKMPISKDFTVILPIVSGNVTESFAKNELFDF
                     VTYITNDSFWNAQIDQIYIKENKKIELVPKVGLYTITLGTLDDYETKLKKLYAFYKTY
                     PEFALTDTYSNINIEYKKLLYATKK"
     gene            complement(3017..4393)
                     /gene="murC"
                     /locus_tag="PLD76_02000"
     CDS             complement(3017..4393)
                     /gene="murC"
                     /locus_tag="PLD76_02000"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU26811.1"
                     /translation="MKSCQQNYYFLGIGGIGMSALARYFKQLGHQVGGYDATENTLTQ
                     KLESEGMEIHYNDSVNTIPVKYMDKENTTIVYTPAIPHDMSERVFFEKAGFTMLKRAE
                     ILGEITRSKKALCVAGTHGKTTTSTILAHILTQSSLGTNAFLGGISKNYSTNLLVNAS
                     SDIVVVEADEYDRSFHQLSPHMAIITATDNDHLDIYQDRENLLSSFQHFTSLIQENGC
                     LLLKKGIELVPSTKNSVKIYTYSANEVADFYATNILIENEKILFDYVTPTFTIEKIKL
                     GVPILINVENAVAAMTIAYLNGVTKEELQHAIETFKGIERRFDFQLQTENVRFIDDYA
                     HHPEELKASIQSIRKLYPNKSICGIFQPHLYTRTRDFATEFANSLSLLDSVILLDIYP
                     ARELPIKGVSSKMIFKHIQCKNKSICSKEDLLEEIKKYNFDVLITLGAGDIDKMVPLI
                     TQQLKKQL"
     gene            complement(4472..5545)
                     /gene="murG"
                     /locus_tag="PLD76_02005"
                     /pseudo
     CDS             complement(4472..5545)
                     /gene="murG"
                     /locus_tag="PLD76_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
     gene            complement(5555..>6036)
                     /locus_tag="PLD76_02010"
     CDS             complement(5555..>6036)
                     /locus_tag="PLD76_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU26812.1"
                     /translation="NSTQRDFLPLAFSDFIYAIVIEEYGLAGGIFIMLLYLIILFRSG
                     RIATQCDKAYPTFMVVGLSLLIVIQAFISIAVASHLGPVTGQPLPLISRGGTSIIMTS
                     VYLGIILNISQYIKKEEKVSNNTTNAPADEAQNEVVSDSEDTAETASDTETRQTDIK"
BASE COUNT         1721 a         1069 c         1001 g         2245 t
ORIGIN      
        1 caataatggt tatttttatt gattcttcca gattttcatc gtatgtagca ccccaaataa
       61 cttctgcatc tttagcaata ttgtttaaga aatgtttaat ctcaatggtt tcagtcatgt
      121 cgagttcatt ttctttggag gtatatacat gtaatagtat gcgtttggct ccttttatat
      181 cgttattatt gagaagagga gagttcaatg catcgtgaat ggctttactt acacgttttt
      241 cgccactagc aaaccctgta ttcataatag caaaaccacc tccttttaaa atagtttgta
      301 catcagcaaa gtccacatta atatatcctg gaatggtaat tatctcggca attcccttgg
      361 ctgcatctgt taggatatta tcagctttgg caaaggctgt tgacattttt aaatctggaa
      421 aaatatcaat aagacgttcg ttattaatca ctaacagagc atctacatgt ttgctcattt
      481 cgcgaacccc attcagtgct ttctgaatct ttttttctcc ttcaaattca aaagggattg
      541 taacaattcc tactgttaag atagatttgt ttttggctat ttctgcgata acaggggcag
      601 caccagtacc tgttcctcca cccattcctg ccgtaataaa taccatttta gtactatcat
      661 ccaatagttc ttcgattttg tccatggttt caagggctgc ttcgcgagct acctctggta
      721 tattacccgc tcccagtcct ttgtctccta gttgtatttt aatagggatt ggagagcgtc
      781 ttaaatcttg gtaatcggta ttacatacag caaatgtaac gtccttaata ccttgattaa
      841 acatatgatt tacagcattg ccaccacctc cacctacacc aattactttt ataattgtat
      901 cagcatttgt tgaaaaatca aaatttacca tgatagttat ttttagtttg ttgtcttatt
      961 gaattgtatc actattgtcg gtaaataaat ctgttaatcg ttgtgatgca ctatcggcaa
     1021 aacgtacgaa taaatttttc ccagtttttt ttgttttttt cgatggtata aatttttctt
     1081 caatagtttc tgatacgcaa ttttctgttc ccattgataa caagccaata atttgactgt
     1141 aagatggttt tttaaaatca tccgaaatag aatcttctgt aagtaaatgt acaccaacag
     1201 tagtacgaac gtccacacca ctcagcgatt gtaccaaagc tctcatattc ttgagttgag
     1261 ctcctcctcc agtaataatg ataccggcac cgagttgttt tatgtatcct ttttgtttta
     1321 atggggtaat gatgaaattt aaaatttcac ttgcccttgc ttcgataatt tgagctaaaa
     1381 atgtgctttg tatcgatttt tcttcttgtt cgtgagctgc tttagcgata ataatacgct
     1441 tatcaggcac ctctaaagtt gccattgcat tgcctagttt ttgctttagt ttctcggctt
     1501 gaggttccgt taaattaagt tctcttatat cgtttgtaat tgttttagct cccaatggaa
     1561 tgacacctat ttctctgagg taattttttg taaatacact atacgaagtt gtactccctc
     1621 caaaatcaat tatcatacag ccaagttctt tttcgtcatc cgaaataagt ccgtttgctg
     1681 tagcaatggg agagggaaat gtgctaatta tttctaattg tgatttttct gccaagagtt
     1741 cttttacttt gttgattcct gtattaattt cgctttttgt ataaataatt ttgtaacgtc
     1801 cttctaattg tgtgcatgct atacctgcag gatctgctac ccactcatca tcaactaaat
     1861 attcctgagt gattacatct aagatatcaa actcagtaga ttgtagttta aagttctctt
     1921 gatacaattt atcaagcaaa ttttgttcta caatttcgtt ataacctaat gtacgttttg
     1981 ttgtgttttc tttgcaacgc aaatacctgc cacctatacc aatatatgct ttggttaggt
     2041 ttaacttagt cttgttacgt aattttgtaa caatactatt gataataaaa gctgtgtctg
     2101 ccacattaaa tatagcccct tgtttaatgg tagttggctt ggtttcctct tcttcgtatg
     2161 ccacaattga gagctttcct tcggttgttt tttgagctac tgcaccaatt attttggttg
     2221 tacccaaatc aaaagctagg atgtaaggag tagatgtgtt catagtgttg gaattatttt
     2281 ttagttgcat atagtaattt cttgtattca atattgatat ttgagtaagt gtctgttaaa
     2341 gcaaattcag gatatgtttt ataaaatgca tatagttttt ttaatttggt ttcataatca
     2401 tcaagtgttc ctaatgtaat agtgtataat cctacttttg gcactaattc aattttttta
     2461 ttttccttga tgtatatttg atcaatttga gcattccaaa atgaatcgtt ggtgatatat
     2521 gttacaaaat caaagagttc gtttttggca aaactttctg ttacattacc agatacgata
     2581 ggcaatataa ccgtgaaatc tttagatatt ggcatctttt ttctgtcttg atcaatgtaa
     2641 taagaataga atggtgtaat aatacgaaca ataggttggc gttgccatat gcgtatgacc
     2701 acatctccat gattggttcg ataacactct acccgtttta catagttcat ctctttgatt
     2761 ttttcttcaa tagcatgtgt aaatgcgaaa tcaatttttc gtcctatcgg atatatattg
     2821 ttatctgtta attgtttttc aatttctttg cgtttaataa aattataatc aacactatca
     2881 attttgaaaa tatgtattgt actacacact tgctcttgtg gtgtatgggt aaaggagatt
     2941 gctgcgtaaa ttaaatacga cagaaacact accgaaacta ctataagagc aatcttttta
     3001 atcattttta tatcaattaa agctgttttt taagttgttg agtaattagt ggcaccattt
     3061 tatctatatc tccagctccc aatgtaatta agacgtcaaa attatacttt tttatctcct
     3121 ctagcaaatc ttctttgcta caaattgact tatttttgca ttgtatgtgt ttgaatatca
     3181 tttttgaact tactcctttg ataggtagct cacgagcagg ataaatatcc aataaaataa
     3241 cactgtcaag gagcgaaagg ctattcgcaa attcagttgc aaaatcacgt gttctggtat
     3301 acaaatgcgg ttgaaaaata ccacaaattg acttatttgg atacagttta cgtattgatt
     3361 gtatagaggc ttttaattct tcgggatgat gggcataatc gtcaataaaa cgtacatttt
     3421 ctgtttgtaa ttgaaaatca aagcgtcttt ctattccttt aaatgtttca atagcatgtt
     3481 gtaattcttc tttagtaaca ccgtttagat atgctatggt catagcagct actgcatttt
     3541 ctacgttgat taaaatagga actcctagtt tgattttttc tattgtaaaa gtaggcgtaa
     3601 cgtaatcaaa aagaattttc tcattttcaa ttagaatgtt tgtagcataa aaatctgcta
     3661 cttcatttgc tgaataggtg tatatcttta ccgaattctt tgtgctaggc actaactcaa
     3721 tacctttttt aagcaacaag catccatttt cttgtataag tgatgtaaag tgttgaaaac
     3781 tagatagtag gttttctcta tcttgatata tatctaaatg gtcattatcg gtggctgtaa
     3841 taatagccat atgtggagat aactgatgaa atgatctgtc atactcatct gcttccacta
     3901 ctacaatatc tgatgaggca ttcaccaata aatttgtgct gtaatttttt gaaatacctc
     3961 ctaaaaaagc attggttccg aggcttgatt gtgtgagaat atgggcaagg atagttgaag
     4021 tagtcgtttt tccgtgtgtt ccagctacac ataaggcttt ttttgaacgt gtaatttcac
     4081 ctaatatttc tgctcttttt agcattgtga aaccagcttt ttcaaagaaa actcgttcgc
     4141 tcatgtcgtg tggaattgca ggtgtatata cgatagtcgt attttcctta tccatgtatt
     4201 tcacaggaat ggtatttaca ctatcgttgt aatgaatttc catcccttct gactctaatt
     4261 tttgtgtaag tgtattttca gtagcatcgt accctcctac ctgatgccca agttgtttga
     4321 aatagcgagc taaagcactc ataccaatac ccccaatacc taaaaaataa taattttgtt
     4381 ggcacgattt tattttttta gtcgttgatt ttcccttttt ttgtatgtta tgtgatacat
     4441 caataccaat tatttttaag atttctttag caatgtgatt agctgcttct ggtttagcaa
     4501 aatgttcaat atttttgcta agatatgtca gtttggtatc gttgctaatg agtgtaagtg
     4561 ctgttgaaat aagaaactct ggtgcttctt ggtcgcttat cataacagca gcttgttttt
     4621 cgaccaacgc cattgcattt tttgtttgat gatcttcaga aacattgggc gatggcacca
     4681 agatacatgg tttttgtagc atgcttaatt ctgatataga gcttgctcct gcacgcgaaa
     4741 taaccaagtc ggctatacta taagccaagt ccatacgagc cacaaaatca gtaataacga
     4801 tgctttttga atcaatatgg cttgtttgtt gttttatact attgaaataa tacttccctg
     4861 tttgccatat aacttgtatg ccagtatctg ctagctgatt aatatgttgc aatacgcttt
     4921 ggtttatagt gcgagctccc aagctaccac ccacaatgag aagggttttt ttagtaggga
     4981 gaaagccaaa ctcgcaatac gcttcttctt tgttttgact attttctaat aattgttgac
     5041 gtacaggatt tcctgtaagt gtaattctat ctgatggaaa aaatcgatgc atattatcat
     5101 aagctacaca tatgcgtgaa gcttttttgg caagcaattt attggtaact cctgcatacg
     5161 agttttgctc ttgaataact gttggaatac ctaatttatt tgccatatac aatgtaggtc
     5221 cactagcata accccctaca ccaactacaa tgtcggggtt aaacgatttg attgtttttt
     5281 ttgctttata taaactttta atcaaattgg gaaaaaatgc tatattacgt ttcatatccg
     5341 aacggtcaaa tccgcttact ggtaacccaa caataggata tccagctgca ggaactttct
     5401 ccatttccat tttattctca gcacctacaa ataggatgtc tgcttctggc aatacttctt
     5461 ttaggctatt ggcaatagaa atggcaggaa aaatgtgtcc tcctgttcct cctccactaa
     5521 taatgatacg tacgtgttct ttcatagatg tatgctattt aatatcggtt tgtcttgttt
     5581 ctgtgtctga tgctgtttca gccgtatctt cactgtctga cacaacttca ttttgtgctt
     5641 cgtcagcagg agcattcgta gtattgttgg atactttttc ttcttttttg atatattgac
     5701 taatatttaa tatgatacct aaatacacac tagtcataat aatagatgtg ccacctcgac
     5761 taatgagcgg tagtggttgt ccagtaactg gacctaaatg agacgccaca gctatgctta
     5821 taaaagcttg aattacaatc aataaagata gccctacaac cataaatgta gggtaggctt
     5881 tgtcgcattg ggtagcaatt cttccagatc ggaataaaat tattaggtac agaagcataa
     5941 taaatatccc tccagctaaa ccatattctt ctatcacaat agcataaata aaatcagaga
     6001 aagccaatgg tagaaaatct ctttgtgtac tatttc
//
LOCUS       DAOSMY010000050         5920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_13597_length_5920_cov_4.157374, whole genome shotgun sequence.
ACCESSION   DAOSMY010000050 DAOSMY010000000
VERSION     DAOSMY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13597_length_5920_cov_4.157374"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLD76_02015"
     CDS             complement(<1..436)
                     /locus_tag="PLD76_02015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843366.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU26813.1"
                     /translation="MERQAFQKIYTHLTNITKATVTLEAGDVGYEEMAIVHGRLAQVV
                     KIMGKMITLQVFSGTEGIPTDAEILFLGRPPQLKVSDELSGRFFNAYGDPIDGGPEIN
                     GEIRDIGSPSVNPVRRKQPSQLIATGIAGIDLNNSLVSGQKIP"
     gene            complement(450..2222)
                     /locus_tag="PLD76_02020"
     CDS             complement(450..2222)
                     /locus_tag="PLD76_02020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU26814.1"
                     /translation="MERKNKTTGKVVGIVANLVIVETNGPVAQNEICFIHLGQENLMA
                     EVIKIVGIKAYVQVFESTRGLKVGTSVTFEGHLLEVTLGPGILSKNYDGLQNDLDKMK
                     GVFLTRGEYTDPLERDSLWNFSPIAKVGDTVYAGSWLGEVMENSIPHKIMVPFKLEKK
                     YTVRSIVQAGTYTVDTTIATIKDDEGNEIAISMVQKWPVKMPIKAYDNKPRPFKLLET
                     GVRCIDTLNPITEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIIAACGERANEVVEV
                     FTEFPELEDPRTGRKLIERTIIIANTSNMPVAAREASVYTAMTLAEYYRSMGLKILLM
                     ADSTSRWAQALREMSNRLEELPGPDAFPMDLSAVISNFYARAGFVYLPNGTTGSITFI
                     GTVSPAGGNLKEPVTESTRKAARCFYGLSQARADSKRYPAIDAIDSYSKYLEYPEVQT
                     YLEKNLGKEWLGQVLQAKDILLRGKEAMEQINILGDDSVPLVFHDKYWKSELIDFIIL
                     QQDAFDKIDASCPMNRQQYMLTSVLKVANTEFKFDGFEEIGGFFKSIINIYKQMNYSE
                     FQSEKFTQYESELNTLVSNKQVNE"
     gene            complement(2206..3069)
                     /locus_tag="PLD76_02025"
     CDS             complement(2206..3069)
                     /locus_tag="PLD76_02025"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOU26815.1"
                     /translation="MSKNYYALVAGLPDIMAQDKKLLYTSVQMRDIISEEVSEEDMSL
                     VQLLFLPFDHLNIVNALFKKQVSWDERGNLSSEIVEQLKDKKNIESVDLENVPSYIRD
                     FVERYYVEEQEINFYQAEFLLTEAYFEYLTTHTNPFVAALGYYYISLNNLKVAYNGRK
                     HDVSYDKQLIGENELTHALRKSRTRDFGASTLVDNLESLLQIFEIENTIERELKIDNN
                     TWNFVEENTFFNYFSIERVMAFIVKLLIVERWLLLDEEKGKQMFNQLVTELQDGFQFS
                     HEYTLAYGKKK"
     gene            complement(3072..3683)
                     /locus_tag="PLD76_02030"
     CDS             complement(3072..3683)
                     /locus_tag="PLD76_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26816.1"
                     /translation="MQGKLQELTEKIYQEGVQKAQKEVEILLQAATQKAQNIEQEAQK
                     KAKAIIAEAEKKAELLDKHVQSELKMSVAQSTSALKQDLTNLLTAKAIEAPVKELFEN
                     TAFLQTLVQTVVSSWIAKGTYDLQVILPEKDKKELDAAFKKQLAKELSKGVELIFSDT
                     VKNGFKVVQSKEGYQLSFTDQDFINYFKAYLRPKTTELLFEKE"
     gene            complement(3840..4730)
                     /locus_tag="PLD76_02035"
     CDS             complement(3840..4730)
                     /locus_tag="PLD76_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26817.1"
                     /translation="MKKLHLFFFVLSISFVSLYAQNNTEETAIYYVTLKDKTHYLGTI
                     ESESTNYIVLYNASTGTRTEIPINQIQEKKIIPTKAIKNNEYWFENPNATRYLFGPSA
                     FNLKKGEGYYQNTMLLLNSFNVGITDYFSIGGGIEFLSTFITLSEGNFNPIIYFTPKV
                     GFSVADNIHVGGGVLIANIPQFGETTNSGLGVLYGLGTYGNLDHNVTATIGWGSVDGD
                     AFNKPLITVSGMTRVAKKTALVTENWLIPTDSSYEPIFSYGVRFFGENIAVDLAFINN
                     WDIAQSLLIGVPYVNFAVKF"
     gene            complement(4750..5292)
                     /locus_tag="PLD76_02040"
     CDS             complement(4750..5292)
                     /locus_tag="PLD76_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26818.1"
                     /translation="MKKTLLILLALVAIVVIIYSYYGGFSSIKIDNQATQKEILVYQS
                     LKGDYKQSADVMNTIYQTLIEEYGIETQKGFGIYYDNPQEVQKENLRSDVGCILNLTD
                     SAKIVQLSSKFLIKEIPAKKYLTATFPYKGQLSIFIGICRVYPALHHYVIKNSIPPIG
                     AVMEVYDVPNKQIHYRIVEN"
     gene            complement(5440..>5920)
                     /locus_tag="PLD76_02045"
     CDS             complement(5440..>5920)
                     /locus_tag="PLD76_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOU26819.1"
                     /translation="ITYTNFGPGMSMGHSVVARSKEGVKNALSMTIPTGTGVHRRMVY
                     VELENGAKLEKVTEAIKTDSYFSKDETHVMEVASVDNLQDMGHGVLIERKGVSGKTQN
                     QLFQFSMQINNPALTAQILISCARAVTKQAPGAYTMPEIAPLDYLYGDRETLIKTLV"
BASE COUNT         1752 a         1091 c          993 g         2084 t
ORIGIN      
        1 agggtatttt ttgtcccgat accaagctgt tatttaggtc gatacctgca attcctgttg
       61 ctattaattg cgatggttgt tttctgcgaa cggggtttac tgatgggcta ccaatatcgc
      121 gtatttcgcc attaatttca ggaccaccat caatagggtc gccataagca ttaaaaaaac
      181 gcccacttag ttcgtcgctt acttttagtt gtggtggacg acctaaaaaa agtatttcag
      241 catcagtagg tatcccttct gtaccagaaa atacttgcaa ggtaatcatc ttgcccataa
      301 tttttactac ttgagccaat ctaccatgta cgatagccat ttcttcgtaa cctacatcgc
      361 cagcttctaa tgttactgtg gctttggtta tattggttaa gtgggtatat attttttgaa
      421 aagcttgacg ttccatattt tatcttttct tattcgttaa cttgtttgtt gcttacaagc
      481 gtatttagtt ccgattcgta ttgagtaaat ttttctgact gaaattcgct ataattcatt
      541 tgtttatata tattgatgat tgacttaaag aaacctccaa tttcttcaaa accatcaaat
      601 ttaaattcag tattcgcaac tttcaatacg ctagtaagca tatattgttg tctgttcatt
      661 gggcatgagg catcaatttt atcaaaagca tcttgttgca aaatgataaa gtcaattagc
      721 tctgatttcc aatatttatc atggaatacc aacggcacac tatcatcgcc taagatgttg
      781 atttgttcca tagcttcttt gccacgaagc aaaatatcct tggcttgtaa tacttgtcct
      841 aaccactctt tccctaagtt tttttctaga tatgtttgaa cttcagggta ttcgaggtat
      901 ttggagtaac tatcaatagc atcaatagca gggtagcgtt tactatcagc acgagcttgc
      961 gataagccat aaaaacaacg agcagcctta cgtgtgcttt ctgtaacggg ttcttttaag
     1021 tttcctcctg cgggtgatac agtgccaata aatgtaatag acccagtggt tccgttaggt
     1081 aaatacacaa accctgcacg tgcatagaag ttggatatta ctgctgataa atccatcgga
     1141 aaagcatcag gtccaggaag ttcttccaat cggtttgaca tttcgcgtag tgcctgagcc
     1201 caacgagagg tggagtctgc cattaataat atttttaaac ccatcgagcg gtaatattct
     1261 gccagcgtca ttgcggtata tacagacgct tcgcgtgctg caacaggcat atttgaggtg
     1321 ttagcaataa taattgtacg ttcaatcagt tttctgccag tacgtggatc ttccaattca
     1381 ggaaactctg taaatacttc tactacttcg ttcgcacgtt cgccacaagc ggcaataata
     1441 acaatatctg cttctgcttg tttagaaatg gcgtgttgca atactgtttt tccagttcca
     1501 aaaggaccag gaataaaacc tgtgccacct tcagtgattg gattgagggt atcgatacaa
     1561 cgaacacctg tttcgagtag tttaaagggt cttggcttgt tatcgtatgc tttgataggc
     1621 atttttacgg gccatttttg caccatgctg atggctattt cgttgccttc atcatcttta
     1681 atagttgcaa tggtggtatc aacggtataa gtacctgctt gtacgataga acgaacggta
     1741 tatttttttt ctaatttaaa tggtaccata attttgtgag gaatactatt ttccatcact
     1801 tcgcccaacc atgatccagc atatacggta tcgcccactt ttgcaatagg agagaagttc
     1861 cataaagaat cgcgttctaa tgggtcagta tattcgcctc tagttaagaa tacacccttc
     1921 attttgtcta aatcattttg caaaccgtcg taattttttg acaaaatacc aggaccaagc
     1981 gttacttcta ataggtgtcc ttcgaatgtt acactcgtac cgacttttag tccacgtgtg
     2041 ctttcaaaaa cttgaacgta tgctttgatt ccaacaattt taattacctc agccattaaa
     2101 ttttcctgac caagatgaat aaagcatatt tcattttgag caacgggtcc attggtttct
     2161 acaatcacca aattggctac tataccaaca actttgccag tagttttatt ttttctttcc
     2221 atatgctagt gtatattcat gcgagaactg aaatccatct tgcagttctg ttactaattg
     2281 attaaacatt tgttttcctt tttcctcgtc taagagcagc catctttcta caatgagcag
     2341 tttaacaata aaagccataa cacgctcaat ggagaagtaa ttgaagaaag tattttcttc
     2401 aacaaaattc cacgtattgt tgtcaatctt tagctctctt tcaattgtat tttctatctc
     2461 aaaaatttgc aacaggcttt ccaagttatc caccaaagtt gatgctccaa aatcgcgtgt
     2521 gcgacttttt ctcagggcat gtgttagctc attttctccg attaattgct tgtcatacga
     2581 tacatcatgt tttcgcccat tataagctac tttaaggtta tttagtgata tatagtaata
     2641 gcctaatgca gcaacaaagg gatttgtatg ggttgttaaa tactcaaaat aagcctcagt
     2701 aagtaaaaat tcagcctgat aaaaattgat ttcttgctct tctacataat atctttcaac
     2761 aaaatctcgg atgtagcttg gtacattttc taaatcaacg ctttcaatgt tttttttatc
     2821 ttttaattgt tcaacgattt cagaggaaag atttcctctt tcgtcccaag aaacttgttt
     2881 tttgaataat gcatttacta tattaagatg atcgaatggc agaaacaata attgcaccaa
     2941 cgacatatct tcttcgctca cttcttcgct gatgatatct cgcatctgta cagaagtgta
     3001 aagtaatttt ttgtcctgag ccatgatatc gggtaatccg gctactaatg catagtaatt
     3061 ttttgacatt cttattcttt ttcgaagagt aattcagtag ttttagggcg tagataagct
     3121 ttgaaatagt ttataaaatc ttggtcagta aaacttaatt gataaccttc cttcgattga
     3181 accaccttga atccattttt tacggtatca gaaaaaatca attctactcc cttgctcaat
     3241 tcttttgcta gctgtttttt gaaagcagca tccaattctt ttttatcttt ttctggaaga
     3301 ataacttgca aatcgtatgt tccttttgct atccaactac ttacaacagt ttgaacaaga
     3361 gtttgtaaaa aggctgtgtt ttcaaataat tcctttacag gggcttcaat agctttcgct
     3421 gtaagcaaat tagttaaatc ttgttttagt gctgaagtgg attgagcaac cgacattttt
     3481 aattcggact gtacgtgttt atctagcagt tcggcttttt tttcagcttc ggcaatgata
     3541 gctttggctt ttttctgagc ttcttgttct atattctgag ctttttgggt ggctgcttgt
     3601 agtaaaatct ctacttcttt ttgtgctttt tgcactccct cttggtagat tttttcagtt
     3661 aattcctgaa gttttccttg catattcgat tagttttgaa ataaaatatt cgtttattga
     3721 acaccacgaa gatacttatt tttatttttg aaaaaaaaaa taaacgcaaa aaaatatcct
     3781 aaaattctta tatttaggat atttttcaat tttttattac attttgcaac gctaacaaat
     3841 caaaatttca cagcaaaatt cacatatgga actcctatta gtaaggattg tgcaatatcc
     3901 caattattta taaaagccaa atcgacagca atattctctc caaaaaaccg aaccccatac
     3961 gaaaatatag gttcgtagga agaatctgta ggaataagcc aattttcagt aaccaaggct
     4021 gtttttttag caacgcgagt cattccagat actgttatca atggtttgtt aaaagcatct
     4081 ccatcaacag atccccaacc aatggtagca gttacgttat ggtcaagatt gccatacgta
     4141 ccgagcccat ataatacacc taagcctgaa ttcgttgttt ctccaaattg cggaatattt
     4201 gctataagca ctcctccacc aacatgaata ttatcggcaa cggaaaaacc aactttgggt
     4261 gtaaaataga taatggggtt aaagttaccc tcacttaaag taataaatgt agataaaaat
     4321 tcgattcctc cacctatcga aaaataatct gtaataccaa cattgaatga attaagtagg
     4381 agcatggtat tttgatagta accttcgcct tttttaaggt taaatgccga tggtccaaat
     4441 aaataacgtg ttgcatttgg attttcaaac caatattcgt tgtttttgat agcttttgtg
     4501 ggtattattt ttttttcttg aatttgattg ataggtattt ctgtacgtgt gccagtagat
     4561 gcattgtaca aaacaatgta atttgtactt tcgctttcga ttgtgcctag atagtgcgtt
     4621 ttatccttta aagtaacata gtatatggct gtttcttcag tattattttg agcatacaaa
     4681 gaaacaaagg aaatcgacaa aacgaagaaa aataggtgta attttttcat agttatattt
     4741 ttactaaaat taattttcaa caattcgata atgaatttgt ttatttggaa catcatatac
     4801 ctccattaca gcaccgattg gaggtatact gtttttgata acgtaatgat gtagggcagg
     4861 ataaactcta catattccta taaaaattga caattgtcct ttgtatggaa aagtcgcagt
     4921 taaatatttt tttgctggta tttctttgat caaaaatttt gacgatagtt gtacaatttt
     4981 agcactatct gtgaggttga gaatacagcc aacatcggag cgtaagtttt ctttttgtac
     5041 ctcttgtggg ttatcgtaat aaataccaaa acctttttga gtttcaatgc catactcttc
     5101 aattagtgtt tgatatatgg tattcattac atcggctgat tgtttgtaat ctcctttgag
     5161 agattggtat acaagaattt ctttttgagt agcttgattg tctattttaa tagaagaaaa
     5221 ccctccataa taactatata ttattactac tatagcaaca agggctaata aaataagcag
     5281 tgtttttttc atatttcagt catttatata tacgtatgaa caaaagtagc aataattttt
     5341 acataatagc aatttgaaac ctaaaaattg aaacaagaaa gagcgaaaaa ctataatctt
     5401 agtttttcgc tctttcttta tttgagtgaa tacagtatat tacaccaatg ttttaattaa
     5461 cgtttctctg tcgccataca aataatcaag aggagcaatc tcaggcattg tataagcacc
     5521 aggagcttgt tttgtaaccg cacgagcaca agaaattaat atttgagcag ttaacgctgg
     5581 attattaatt tgcatgctaa attggaacaa ttggttttgg gttttacccg aaaccccttt
     5641 gcgttcaatt aaaacgccat gacccatgtc ttgcaaatta tcaacactag caacttccat
     5701 aacgtgagtt tcatctttac tgaaatacga gtcagtttta atagcttctg taactttttc
     5761 tagtttagca ccattttcta attccacata caccatacgg cgatgaacgc ctgtacccgt
     5821 agggatagtc attgatagag cattttttac tccttctttg gaacgtgcta ctacagagtg
     5881 tcccatactc atacctggac caaagttggt ataggtaata
//
LOCUS       DAOSMY010000051         5868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_13751_length_5868_cov_3.772579, whole genome shotgun sequence.
ACCESSION   DAOSMY010000051 DAOSMY010000000
VERSION     DAOSMY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13751_length_5868_cov_3.772579"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLD76_02050"
     CDS             complement(<1..208)
                     /locus_tag="PLD76_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26820.1"
                     /translation="MIKQNNIVVKQYVDELRNAHIAFCYFDNKYGVLCDATNENLVHK
                     MYTKLLPQRMYLLVDNVVKLRHYVS"
     gene            401..2125
                     /gene="recJ"
                     /locus_tag="PLD76_02055"
     CDS             401..2125
                     /gene="recJ"
                     /locus_tag="PLD76_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU26821.1"
                     /translation="MTNNWIVKELTTEESEHTEHLIKDLGISPILCKLLVQRGIHTME
                     EAKNFFRPQLSQLHDPFLLNDMDKAVARLNKAMRKKEKILIYGDYDVDGTTAVAVVYK
                     FLQQFYSSIDSYIPDRYEEGYGISYKGIDYAAENGFSLLIVLDCGIKAIEKVRYAKEK
                     GIDIIICDHHTPDEILPDAIAVLDPKRVDSTYPYPHLSGCGVGFKLIQAFAINNQIDF
                     DNITPFLDLLAVSIASDIVPITGENRILMFFGLKQLNANPCVGLQAIIDVCGLRGKEI
                     TTSDIVFKIGPRINASGRMRSGNEAVELLVSKNLSFAKAKSDCIDQYNQERKDLDKKT
                     TDDAKDFIQGNEDFEHRNSIVVYHPEWSKGVIGIVASRLSELYHKPTIVLTLSNGFAT
                     GSARSVTGFDIYKAIESCRDLLENFGGHMYAAGLTMKEDNVSIFTERFDNYVRENITE
                     EQTQPQIDIDAIIRFSDITPKFFNILQQFAPYGPENMKPIFATHKVTDFGSSRLVGKE
                     LEHLKLELTEPNSENVMNGIAFGMGEKFSAHIKDLKPIDICYTLEENVFNGNKTIQLM
                     IKDMKINE"
     gene            2146..4050
                     /locus_tag="PLD76_02060"
     CDS             2146..4050
                     /locus_tag="PLD76_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOU26822.1"
                     /translation="MYHEILHTYWGYSEFRSLQLDIIQSIGNGKDTLGLMPTGGGKSL
                     TFQVPALAKEGLCLVITPLIALMKDQVENLKKRGIKATAIYSGMSYEEMMIAIDNCMY
                     GNYKFLYVSPERLSTRAFLLKIPHLQINLIAVDESHCISQWGYDFRPSYRQIASIREH
                     LPTVPILALTATATPEVVRDIQTQLKFAAPNVFQKSFVRSNLIYIVRQTDDKLQQLLQ
                     ILQKVPGTSVVYVRNRKKTKEIADFLNENNIPANYFHAGLSNNTKDERQQKWKNDECR
                     VMVSTNAFGMGIDKPEVRTVIHLDLPDSIENYFQEAGRAGRDEKKAYAILLYSNADKV
                     KIEKRIRDNFPEKEKIKEIYEAISNYLQIPVEGKEGAVYDFEIFDFCAKFHFPALLVH
                     SAINILEQAEYICYTEEVDSNSRILFTVSRDDLYAINNSHIHSEKIIHALLRSYTGLF
                     TDYAYINEEIIAQRAGLTVDETIQTLIFLAKTKVIQYIPRKKTPFITYTQQRQEKRYL
                     KIPREVYEEKKERYVKRIESMIAYATNTTVCRSRMLLHYFGESNTHDCDQCDICLTRK
                     KNDLANQEFQQLCTTIKTTLLEKALTIHELSAKYNHQKEKIIKTTRWMLDQGEIYCDD
                     TLKLHLNKTK"
     gene            4047..4958
                     /locus_tag="PLD76_02065"
     CDS             4047..4958
                     /locus_tag="PLD76_02065"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOU26823.1"
                     /translation="MKAELTILGCGSAMPSYGRFSASQVLSVRDKSFMIDCADGTLFR
                     MRETAIKTNRLNHIFISHLHGDHCFGLMAVLSTLGLMKRTADIYIHAHPDLENLLQPH
                     LRYFCNDLTYKIFFVPYNPLQSEIIYEDKSLTVTTIPLRHGIPTCGFLFKEKPQQLHI
                     KRDMIDFYQVGIKDLNAIKDGKDYITNQGETIPNSFFTTPASPQKSYAYCCDTAYSEK
                     IIPIIEGVDCLYHDATFAENEVLRAKKTLHSTAKQAATIASKAQVKQLLLGHFSARYP
                     HVSVILNEAQTIFANSIIAEDKKTYTF"
     gene            5084..>5868
                     /locus_tag="PLD76_02070"
     CDS             5084..>5868
                     /locus_tag="PLD76_02070"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU26824.1"
                     /translation="MSFIADKIAFDGLTFDDVLLIPAFSEVLPREVNLSTKFSRNIEL
                     KIPFVTAAMDTVTEAKMAIAIAREGGIGVIHKNMSIEEQARQVAIVKRAENGMIYDPV
                     TIKRGSTVADALAIMAEYKIGGIPVVDDNKILVGIVTNRDLRFERNLDRFIDEVMTKE
                     NLVTTKQSTDLESAAQILQQYKIEKLPVIDNNGKLIGLVTYKDITKAKDKPMACKDAK
                     GRLRVAAGVGVTDDTLDRMQALVDAGVDAIVIDTAHGHSISVI"
BASE COUNT         2114 a         1062 c          985 g         1707 t
ORIGIN      
        1 cagacacata atgccttaat ttaacgacat tatctaccaa taaatacatt cgttggggta
       61 atagtttggt atacatttta tgtactaaat tttcgttcgt agcatcacaa agcacaccat
      121 atttattatc aaaataacaa aaagctatat gagcatttct caattcatct acgtactgtt
      181 taaccacaat attattctgc tttatcattc tttacattca agtcgaaaaa tatcttgtaa
      241 acattttttc acactcacaa gaagttgttt ttactatttt attgtatctt tacgatttga
      301 tttattcttt ttactttaaa aaagtgaaaa ttgaattatg cgtacaaata tacaatgatt
      361 ttgtacagtt tttatcacag cgataataaa aacacactat atgacaaaca attggattgt
      421 aaaagagctt acaacggaag aatctgaaca cactgaacat ttaattaagg atttgggaat
      481 aagtccaatt ttgtgcaaac tactcgtaca aagaggcata cacacaatgg aagaagcaaa
      541 aaactttttc cgacctcaat taagtcagct acacgaccct ttcttactca acgacatgga
      601 caaagcagta gcacgcttaa ataaagcaat gagaaagaaa gaaaaaatct tgatatatgg
      661 cgattacgat gtagatggta caacagcagt agctgtggta tataaatttt tacaacagtt
      721 ttattcaagt attgattcgt atatcccaga tagatacgag gaaggctacg gcatttcata
      781 caaaggaatt gattatgctg cagaaaatgg tttttctctg ttaattgtac ttgactgtgg
      841 cattaaggct atagagaaag tgcgttatgc caaagaaaag gggattgata ttattatttg
      901 cgaccaccat actcctgacg aaattcttcc tgatgctatt gcagtattag accctaaaag
      961 agttgattct acgtatccat atcctcattt atctggttgc ggtgttgggt ttaaactcat
     1021 acaagctttt gctataaata atcaaattga ctttgacaac attacccctt ttcttgattt
     1081 attagccgtt agtattgcat ctgacattgt ccccattaca ggcgaaaata gaatcttgat
     1141 gttttttggc ttaaaacaac taaacgcgaa tccatgtgtt gggctacaag ccattattga
     1201 tgtatgtggc ttgagaggca aagaaatcac tacttctgat atcgttttta aaataggtcc
     1261 acgtattaat gcatcaggca gaatgagatc tggcaacgaa gcagtagagt tattagttag
     1321 taaaaatcta agctttgcta aggctaaaag cgactgcata gaccaataca atcaagaacg
     1381 caaagatttg gacaaaaaaa caacagatga cgctaaagac ttcatccaag ggaacgaaga
     1441 ttttgaacac agaaactcca tcgtcgttta tcatcctgaa tggagtaaag gagttattgg
     1501 tattgtggca tctaggctga gcgaattata ccacaaacct actattgttc ttacgctttc
     1561 aaatggtttt gctactggtt ctgcacgttc tgttactggt tttgatattt ataaagcaat
     1621 agaatcgtgt cgggatttgt tagaaaactt tggaggacat atgtatgcag ctggattgac
     1681 catgaaagaa gacaatgtgt caatttttac agaacgtttt gataactatg tccgcgaaaa
     1741 tattaccgaa gagcaaacac aacctcaaat agatattgac gccattatcc gttttagtga
     1801 tataacgcca aaatttttta atatattaca acaatttgca ccatacggtc ctgaaaacat
     1861 gaaaccaatc tttgcaaccc ataaagttac agattttggc tccagccgac tagtaggaaa
     1921 agaattagaa catctaaaac tagaactaac tgagcccaac tcagaaaatg taatgaatgg
     1981 aattgctttt gggatgggag aaaaatttag tgctcacatt aaagacctca aaccaatcga
     2041 tatttgttat acgttggaag aaaacgtctt taatggaaat aaaaccatcc aactaatgat
     2101 aaaggatatg aaaattaatg aataaatagc agttaataac ctaacatgta ccacgaaatt
     2161 ttacatacat attggggata ttctgaattc cgttcattac aacttgatat tattcagtct
     2221 attggaaacg gcaaagacac cttgggctta atgccaactg gtggagggaa atcgcttact
     2281 tttcaagtac cagctctagc aaaagaaggt ttgtgtttgg tcattactcc tcttattgct
     2341 ttgatgaaag accaagtaga aaacctgaag aagagaggaa tcaaagcaac tgccatatac
     2401 agtggcatga gctatgaaga aatgatgatt gctatagata attgcatgta tgggaattat
     2461 aagtttttat acgtttctcc cgaacgactt tcaactcgag cttttttact taaaatacct
     2521 catctacaaa tcaatcttat tgccgttgac gaatctcatt gcatttcaca atggggatac
     2581 gattttcgtc cttcatatcg tcaaatagca tctattagag aacatttacc tacagtacct
     2641 attttagctc ttacggctac agcaaccccc gaagtagtac gcgacattca aacacaactc
     2701 aaatttgctg caccaaatgt gtttcaaaaa agttttgttc ggagcaattt aatttatatt
     2761 gtacgtcaaa ccgatgataa actgcaacaa ttactacaaa tacttcaaaa agtgcctggt
     2821 acttctgttg tatatgtaag aaacagaaaa aaaacaaaag aaatagctga ttttttaaac
     2881 gaaaacaata tccctgctaa ttatttccac gcaggactta gtaataatac caaagatgaa
     2941 cgccaacaaa aatggaaaaa tgatgaatgt cgtgtaatgg tatctaccaa cgcatttggt
     3001 atgggcatag ataaaccaga ggtacgtact gttatccatt tagacttacc agatagcatt
     3061 gaaaactatt ttcaagaagc tggtagagct ggtagagacg aaaaaaaggc gtatgccata
     3121 ctactgtata gtaatgctga taaagtaaaa attgaaaaaa gaatacgaga caacttccct
     3181 gaaaaagaaa aaattaaaga aatttacgaa gccatatcca attacctgca aatacccgta
     3241 gaaggaaaag agggagctgt atatgatttt gagatatttg atttttgtgc caaattccac
     3301 tttccagctt tacttgttca tagtgcaatc aatattctag aacaagcaga atatatatgc
     3361 tatacagaag aagtagacag taattcgcgc attctattta ctgtttcgcg tgatgatttg
     3421 tatgcaatta acaactccca tatacattca gaaaaaataa tccatgctct tttacgctca
     3481 tacacaggac tatttacgga ttatgcatac atcaacgaag agataattgc acaacgtgct
     3541 ggattaacag tagacgaaac aattcagaca ctaatctttt tagctaaaac taaagttatt
     3601 caatatattc ctcgaaaaaa aacacctttt attacctaca cccaacaacg acaagaaaaa
     3661 cgatatctaa aaattccgag agaagtatac gaagaaaaaa aagaacgtta tgtaaagcgt
     3721 atcgaatcaa tgattgccta cgctacaaat actaccgttt gccgaagccg aatgcttttg
     3781 cattattttg gcgaaagcaa cacacatgat tgtgatcagt gtgatatttg cttgacacgt
     3841 aaaaaaaatg atttagccaa ccaagaattt caacaacttt gcactaccat aaaaacaact
     3901 ttactagaaa aagcactaac catacacgaa ttgtcagcaa aatacaacca ccaaaaagaa
     3961 aaaatcatta aaactacacg atggatgctt gaccaaggag agatatattg tgatgatacg
     4021 ttgaaattac atcttaacaa aacaaaatga aagctgaatt aaccatatta ggatgcggat
     4081 ctgctatgcc atcgtatggc agattttccg cttcacaggt gttatctgta cgcgataaaa
     4141 gtttcatgat tgattgtgct gatggcactc tctttagaat gagagaaaca gctatcaaaa
     4201 caaatcgtct caatcacata tttatttcgc atttacatgg agaccactgt tttgggctta
     4261 tggctgtatt atctacctta ggcttgatga aaagaaccgc tgatatatat attcatgccc
     4321 accctgattt ggaaaatttg ctacaacccc acttacgcta tttttgtaac gatttaacat
     4381 acaaaatctt ttttgtaccc tacaatccgc tacaaagcga aataatatac gaagataaat
     4441 cactaacagt aacaaccatc ccattacgcc atggcatacc tacttgtgga tttcttttca
     4501 aagaaaaacc tcaacaactc catatcaaac gagatatgat tgatttttac caagtaggaa
     4561 taaaagattt gaatgccatt aaagacggaa aagattacat aacaaaccaa ggagaaacaa
     4621 taccaaactc cttttttaca actccagcca gcccacaaaa aagctatgct tattgttgtg
     4681 atacagcata ttctgaaaaa ataataccca taatagaagg ggtagattgt ttgtatcatg
     4741 atgctacttt tgccgaaaat gaagtactca gagcaaaaaa aacattacat tctacagcaa
     4801 aacaagccgc aacaattgcc tcaaaagcac aagtaaaaca attactatta ggacatttct
     4861 cagctcgcta cccacatgta tccgttattt taaacgaagc tcaaacaata tttgccaata
     4921 gtataatagc cgaagataaa aaaacatata ctttttagac aaaaaataac acttactatc
     4981 caaaaatacg cctttagcta actaatttta acgtagatag aataccgtaa gttcgatatt
     5041 tttttcgtat atttgcacgc aataatttta atacctaaat cgtatgtcgt ttattgcaga
     5101 taaaatagct tttgatgggt taacatttga tgatgtactc ctcatccccg cattttccga
     5161 agttcttcca cgcgaagtaa atctttctac aaagttttct agaaatattg aattaaaaat
     5221 tccttttgta acagctgcca tggataccgt aaccgaggca aaaatggcta ttgcaattgc
     5281 tcgagaagga ggtattggtg ttatccacaa aaacatgtca atagaggaac aagctcgaca
     5341 agtagccatt gttaaacgtg ccgaaaacgg tatgatttac gaccccgtta ccattaaacg
     5401 aggatctact gtagctgatg cccttgctat tatggcggaa tacaagattg gaggaatacc
     5461 cgtagtagat gataacaaaa ttctagtagg cattgttaca aaccgcgatt tacgttttga
     5521 acgtaatctt gaccgtttta tcgacgaagt aatgacaaaa gaaaatttgg taacaaccaa
     5581 acaatctacc gatcttgaat cagcagctca aatactacag caatataaaa ttgaaaaatt
     5641 acctgtaatt gataataatg gcaaacttat tggattggtt acatacaaag acataactaa
     5701 agccaaagac aaacctatgg cgtgtaaaga tgccaaagga cgattgcgtg ttgctgcagg
     5761 cgttggagta actgacgata ccttagatag aatgcaagcc ctagtagatg caggggtaga
     5821 tgctattgtt attgatactg ctcacggaca ctcaatttct gtaattga
//
LOCUS       DAOSMY010000052         5855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_13786_length_5855_cov_3.143103, whole genome shotgun sequence.
ACCESSION   DAOSMY010000052 DAOSMY010000000
VERSION     DAOSMY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13786_length_5855_cov_3.143103"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PLD76_02075"
     CDS             complement(<1..620)
                     /locus_tag="PLD76_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOU26825.1"
                     /translation="MDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDVIEA
                     GFPVSSPGDFKSVVEISKAVTWPTICALTRGVQKDIEVAAEALQYAKNKRIHTGIGTS
                     DYHIKHKFNSTREEILERAVAAVKYAKKYVEDVEFYCEDAGRSDNEYLARVVEAVIKA
                     GATVVNIPDTTGYCLPSQYGEKIKFLMENVKGVHNAILSTHCHNDLG"
     gene            complement(938..1840)
                     /locus_tag="PLD76_02080"
     CDS             complement(938..1840)
                     /locus_tag="PLD76_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU26826.1"
                     /translation="MQQGERYKEIRNVTLIGFVVNLLLTLAKLFAGIVGKSSAMIADG
                     VHSLSDFATDIVVIVFMRVSDKESDENHKYGHGKFETFAILIISICLFVVGIGLLIGG
                     IDKIVYFYKGNEIMQPSLLAFYAALVSIALKEALFWYTFKIGKKVNSDAVIANAWHHR
                     SDAFSSIATALGIAGAIFLGEKFRILDPIASVLVSFFIMKVSYELAMPSVKELLESSL
                     PKEKEDEIIDHVLSIPGVITYHHLRTRKVGNLIAIDIHIKLNKHISFVESHDIATDVE
                     SLLRHQYGNQTVVNIHTEPYEEKK"
     gene            1975..2397
                     /locus_tag="PLD76_02085"
     CDS             1975..2397
                     /locus_tag="PLD76_02085"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU26827.1"
                     /translation="MNSSQEYTGTIVKINQETVTVCIKQLAACVSCQTKGACSSADVS
                     SKLIEATINNESFEIGETVRIIGKKNFGLKAVLMAFVIPVFLILLILFVAEYYKKDDL
                     TMVISTLFVLIPYYLILFLLQNKLKDQFMFYVEKIDKK"
     gene            2394..3245
                     /locus_tag="PLD76_02090"
     CDS             2394..3245
                     /locus_tag="PLD76_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOU26828.1"
                     /translation="MNTIFLSLVVLGTIGLLAAIILYVVSQKFKVEENPIIDEVEAAL
                     PSANCGGCGFPGCRGFAVACSEATDLSNLFCPVGGNDTMKTVAHIVGLSVVETKPKIA
                     VLKCNGTCEHRPRTNQYDGAKSCKIIHNLYIGETACHYGCLGGGDCEVACAFDALHIN
                     PVTGIPEIDEEKCTACGACVKACPKLLLELRFKGPKGKRIYVACSNKEKGGIAKKACE
                     VACIGCSKCFKVCPHDAIIIENNLAYIIDDKCRLCTKCVDECPTGSIQKLNFPIKKKE
                     VAEQAQA"
     gene            3295..4632
                     /gene="rsxC"
                     /locus_tag="PLD76_02095"
     CDS             3295..4632
                     /gene="rsxC"
                     /locus_tag="PLD76_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOU26829.1"
                     /translation="MKTFTFGGIHTADMKLSAGKKIQIVPIPSEVIIPLEQHIGAPTN
                     PVVAVGETVKVGQLIGKAVGFISANIHSSVSGKVTKIDTVIDAWGYKRPAVYIAVEGD
                     EWMENIDKKDVVRKEITTKREDIIQKIADAGIVGLGGACFPTHVKLSPPPNTKAELLL
                     INAVECEPYLTCDHQLMLEKAEEIIVGIQILMTVLQVNKAIIGIENNKKDAISLLQQI
                     TSSYSTIEVCPLAMCYPQGGEKQLIEAITKKQVPSGQLPINIGIVVQNVATTYAIYEA
                     VQKNKPLIERVITVTGKKVAKPGNFLVRIGTPLRVPLDMAGGMPEDTGKIIGGGPMMG
                     RAFMNADVPVTKRSAGLLVMPTLESARKEMQNCIRCAKCVTACPMGLEPYLLMSQAEK
                     SMWKEMEETYITDCIECGCCLFSCPSNRPLLDYLRMGKSTVLEQIRNRVQKVN"
     gene            4651..5625
                     /locus_tag="PLD76_02100"
     CDS             4651..5625
                     /locus_tag="PLD76_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU26830.1"
                     /translation="MMNKLIISPAPHIHSGDSISKNMYAVLFALVPAYLVGLYFFGIG
                     AFVVSITAVLSCIIFEFLIQKFIFKTTTTIHDGSAMLTGLLLAFNLPSNLPIWIIIIG
                     ALVAIGIGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTTWPKPIPFNTSYLDAETAA
                     TPLAMIKYNFGNLPEKMDLLIGNMGGSIGEVSAIALILGGIYLLIKRVITWHIPVSML
                     VTTFLFTGILHLINPSLYVSPDIHLLTGGLLLGAIFMATDYATSPMNTVAQLVYGIGI
                     GLITVTIRLFGAYPEGVSFAILIMNGVTPLLNLYVKPKRFGAPKTKKN"
     gene            5652..>5855
                     /locus_tag="PLD76_02105"
     CDS             5652..>5855
                     /locus_tag="PLD76_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26831.1"
                     /translation="MKKLESNLPNMVMVLTAITLIASFLLASVYGVTKAPIELAKEKK
                     KEVAIKNVLPPFNRIENDTIEGIV"
BASE COUNT         1981 a          977 c         1024 g         1873 t
ORIGIN      
        1 cctaagtcgt tgtggcaatg agtagaaagg atagcattat gaactccttt tacattttcc
       61 atcaaaaatt taattttctc tccgtattgt gatggcaaac aatagcctgt tgtatcagga
      121 atgttaacca cagttgctcc agctttgatt acagcctcta caactcttgc taagtattcg
      181 ttatcactac gaccagcatc ttcgcaataa aattccacat cttccacata cttcttggca
      241 tattttacag cagcaacagc acgttctaat atttcttcgc gtgtagaatt aaatttgtgt
      301 tttatatgat aatctgatgt accaatacct gtatgaatac gtttattttt agcgtattgt
      361 aaagcttcag cagctacttc aatgtctttt tgtacaccac gtgtaagtgc acaaattgtg
      421 ggccacgtta cagctttgga tatttctacc actgatttaa agtctcctgg gctagatact
      481 ggaaatcctg cttcaattac atctacacct aaagattcta aggcttttgc tacctgaatt
      541 ttttctactg tattcaactg acaccctggc acttgttctc catctctcaa ggtggtgtca
      601 aaaataaata atctgtccat atcctcacgt atttatattg ttcttacaaa aattacaaga
      661 aaaagcctct cattaccgaa gtaagaaagg ctttctatgt tattattaat tttccacata
      721 ccaaactcac tccgctcatc ctttacagag aagaatacta agtaaaatag tatgtagagt
      781 gctaaatgac ataatcgttg ttttgtttgg caaagatact gtcttttttt tattcaaaca
      841 aactatttaa aagaaaaata ttaaaaaaaa tgtcaaaaaa taactgtatc tttctaaata
      901 aaatcaaata taaaaaataa tgcacatgat taaaccttta ttttttttct tcataaggtt
      961 ctgtatgtat attcacaact gtttgattac catattgatg acgcaacaaa gattctacgt
     1021 ctgtagcaat atcatgagac tctacaaaac ttatatgttt gttcagttta atatgaatat
     1081 caattgcaat gagattacct acttttctag tacgcaaatg atgatatgta attacacctg
     1141 gtatagacaa tacgtggtca attatttcat cttctttttc ttttggtaaa gaagactcca
     1201 acagttcttt tacactaggc attgctaatt cataactgac tttcataata aagaaactca
     1261 ctagtacact agcaattgga tccaagattc taaatttttc tcctaaaaat attgctcctg
     1321 caatacctaa ggctgttgct atagaagaaa atgcatctga acgatgatgc catgcatttg
     1381 caataacagc atcactattc acttttttac caatcttaaa tgtgtaccaa aacaacgctt
     1441 cttttaaagc aatcgaaaca agagccgcat aaaaagccaa cagactcggt tgcattattt
     1501 cattcccctt ataaaaataa acaatcttat cgataccacc tattaacaac cctattccaa
     1561 cgacaaataa gcagatactt attattaaaa tagcaaaggt ttcgaattta ccatgaccat
     1621 atttatgatt ctcatcactt tctttgtctg atactctcat aaaaacaata accacaatgt
     1681 ctgtcgcaaa atcagacaaa gaatgcaccc catcagcaat catagcagaa cttttaccta
     1741 caatacctgc aaaaagtttg gcaagggtaa gcaatagatt aactacaaaa ccaataagcg
     1801 ttacatttct aatttctttg tatcgttctc cttgttgcat ttagttaaaa ttttatatgc
     1861 ttgcaaaggt actctttttg gtaaaataat aaaataaaat aaaaaattaa ctaaaaaata
     1921 cgtataagat ataaaataaa cagtaacttt gtgctttcta aaaatgattt acgtatgaat
     1981 tcttctcagg aatatactgg aaccatcgta aaaataaacc aagaaacagt aacagtctgc
     2041 ataaaacaac ttgctgcttg tgtttcttgt caaacgaaag gtgcttgtag ttctgctgat
     2101 gttagctcta aattaataga agcaactatt aataatgaga gttttgagat tggcgaaaca
     2161 gtacgtataa taggcaaaaa aaactttggg ttaaaagctg ttctcatggc ttttgttata
     2221 cctgtttttc taattttgct tattttgttt gttgcggagt attacaaaaa agatgattta
     2281 acaatggtaa ttagtacatt atttgtgcta ataccatatt atttaatact atttctattg
     2341 cagaataaac tgaaggatca gtttatgttt tatgttgaaa aaatagataa aaaatgaata
     2401 caatttttct ttcattagta gttttgggca ccattggatt gcttgctgcg attattttgt
     2461 acgttgtttc tcaaaaattt aaagtagaag aaaaccccat cattgatgag gttgaagcag
     2521 cattacctag tgccaattgt ggtggatgtg gttttcctgg atgtagagga tttgctgtag
     2581 catgtagtga agcaacagat ttaagtaatt tgttttgtcc tgttggcgga aatgatacca
     2641 tgaaaactgt tgctcatata gtaggattaa gcgtagtgga aacaaaaccc aaaattgcag
     2701 tgttaaaatg caacggaacc tgcgagcacc gtccacgcac taatcaatac gacggagcga
     2761 aatcatgcaa aattattcac aacttataca taggagaaac agcctgtcat tatggttgtt
     2821 tgggtggtgg cgactgcgaa gtggcttgtg cctttgatgc tttgcatata aacccagtaa
     2881 caggaatacc agaaattgat gaagaaaaat gtactgcgtg tggtgcttgt gtaaaggcat
     2941 gcccaaaatt attgttagaa ttacgattta aaggacccaa aggcaaacgc atctatgtag
     3001 catgtagtaa taaagaaaaa ggaggaattg caaaaaaagc atgcgaagta gcctgtatcg
     3061 gatgctctaa atgctttaaa gtatgcccac atgatgccat aattattgaa aacaatttgg
     3121 cgtatataat agacgataaa tgtcgtttgt gtacaaaatg tgtggatgaa tgtccaacag
     3181 gtagtattca aaaattaaat ttccctatta agaaaaaaga agtagctgaa caagcacaag
     3241 cataaaacta agttagtagt atataactat tacgtatatt ctgaaataag acgtatgaaa
     3301 acatttacct ttggaggcat tcatactgcg gatatgaaat tatcggcagg aaaaaaaatt
     3361 caaatagtac cgatccctag tgaagtaatt attcccttag aacagcatat tggggctcct
     3421 acaaacccag tggtagctgt aggtgagacg gttaaagtgg gtcagttaat tggtaaagct
     3481 gtagggttta tttcggcaaa tattcactca tccgtttcag gaaaagtaac caagattgat
     3541 acagttatag atgcatgggg atacaaaaga cctgccgtat acatagcagt agaaggcgac
     3601 gaatggatgg aaaatataga taaaaaagat gtagttcgca aagaaataac aacgaaaaga
     3661 gaagatatta tacagaaaat agcagatgct ggtattgtgg gtttaggtgg agcttgtttt
     3721 cctacacatg ttaagctatc tcctcccccc aatacaaagg cagaattact actaattaat
     3781 gcagtagaat gtgaaccata tttaacttgc gaccatcaat taatgctcga aaaagcagaa
     3841 gaaataattg taggtattca aattttaatg actgtattgc aagtaaacaa agccattatt
     3901 ggcatagaaa ataacaaaaa agatgccatt tctctattac agcaaatcac aagttcatat
     3961 tcaactattg aagtttgccc cttagcaatg tgttacccac agggaggcga aaagcaacta
     4021 attgaggcaa tcacaaaaaa acaagtgcca agtggtcaat tacctattaa tattggtatt
     4081 gtcgttcaga atgtggcaac aacatatgcc atttacgaag cagttcaaaa aaacaaacct
     4141 ttaatagaac gtgtaataac agttacgggg aaaaaagttg caaaaccagg taatttttta
     4201 gtacgtattg gaactccttt acgcgttcca ttagatatgg ctgggggaat gcccgaagat
     4261 actggaaaaa ttataggtgg aggtccaatg atgggacgag cttttatgaa cgcagatgta
     4321 cctgttacga aacgtagtgc tggtttatta gtaatgccta cccttgagtc tgcccgtaaa
     4381 gaaatgcaaa attgcattcg ttgtgccaaa tgtgtaacag cttgtccaat gggtctagag
     4441 ccctacttat tgatgtctca ggctgaaaaa agcatgtgga aagaaatgga agaaacatac
     4501 attactgatt gcattgaatg tggttgctgt ttgttttctt gtcctagtaa tcgaccacta
     4561 ttagactatc tacgtatggg gaaaagcact gttttggaac aaatacgtaa tcgcgttcaa
     4621 aaagttaact aattgaaatc tacacaaagt attatgaata aactaattat ttcgccagct
     4681 ccacatatac atagtggcga ttctatttct aaaaatatgt atgcagtact atttgcatta
     4741 gtacctgcct atttagttgg tttgtatttt tttggaattg gtgcttttgt tgtcagtatt
     4801 actgccgttt tatcttgtat tatttttgaa tttttaattc aaaaatttat tttcaaaact
     4861 accactacta tccatgatgg ttctgccatg cttacgggct tattattagc ttttaacctc
     4921 ccatctaacc tacccatatg gattattatt attggagcat tagtagcgat aggtattggt
     4981 aaaatgagtt ttggaggatt aggtaacaat ccatttaatc cagctttagt tggtcgtgta
     5041 tttttattaa tatcttttcc tgtacaaatg acaacttggc ccaaaccaat accatttaat
     5101 acaagttatt tggatgctga gactgctgct acacctttgg ctatgattaa atataacttt
     5161 ggtaatttac cagaaaaaat ggacttactc attggtaata tgggtggtag tattggagag
     5221 gtatctgcta tcgcattaat tctgggagga atctatttgc tgatcaaacg tgttattaca
     5281 tggcatatcc cagtaagtat gttagttact acatttttgt ttacagggat attgcatttg
     5341 attaatcctt ctttatatgt atctcccgac attcacctgt taactggagg attgctattg
     5401 ggagctattt ttatggctac tgattatgcc acatcgccta tgaatacagt agcacagttg
     5461 gtatacggaa taggtatcgg actgattaca gtaacaatac gtttatttgg agcctatcca
     5521 gagggagtat cttttgctat attgattatg aatggtgtta caccattatt gaatctatat
     5581 gttaaaccca aacgatttgg ggcaccaaaa acgaaaaaaa actaaagtag gtattcaaaa
     5641 taacatacta aatgaaaaaa ttagaatcaa atttgcctaa tatggtcatg gttttgactg
     5701 ctattacctt aatagcatca tttttgttgg caagtgttta tggtgttact aaagcaccaa
     5761 ttgagttggc taaagaaaaa aagaaagaag tcgcaattaa aaatgtactg cctcctttta
     5821 atcggataga aaatgacaca attgagggaa ttgtt
//
LOCUS       DAOSMY010000053         5828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_13860_length_5828_cov_3.689936, whole genome shotgun sequence.
ACCESSION   DAOSMY010000053 DAOSMY010000000
VERSION     DAOSMY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13860_length_5828_cov_3.689936"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLD76_02110"
     CDS             <1..443
                     /locus_tag="PLD76_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOU26832.1"
                     /translation="VDAVREAVKDENAEILIVAAATEADIAELETYEERQMFLEEVGL
                     SESGVARLIKSAYKLLNLETYFTAGVQEVRAWTYEKGWKAPQCAGVIHTDFEKGFIRA
                     EVIKYIDFTTLGSEAACKEAGKMSVEGKEYVVQDGDIMHFRFNV"
     gene            809..2965
                     /locus_tag="PLD76_02115"
     CDS             809..2965
                     /locus_tag="PLD76_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU26833.1"
                     /translation="MKVYQTNEIKNISLVGSSGSGKTTLTEAMLFESGVIKRRGSIEA
                     KNTVSDYFPVEHEYGYSVFPTVFHSEWMGRKLNMIDCPGADDFVGGLITSLSVTDTAL
                     MLINAQHGVEVGTQNQYRQIEKLKKPCIFVVNQLDHEKADFDKTVDDLRQNFGNKVVL
                     IQYPIQTGPAFNAVVDVLLMKLYTWKADGGVPTISDIPANELQRASELHNILVEAAAE
                     HEENLMEKYFADGNLTEDEMRMGIRFGLVHRDIFPVFCISAAKDMGVRRLMEFLGNVV
                     PYVRDSAAPVATKGEEVAPLQENNASIYVFKTSVEPHIGEVSFFKVMSGKVKEGDDLY
                     NATRQSKERLTQIYAVCGSIRTKVDELVAGDIGATVKLKNTRTSNTLNGKNADLQFDF
                     IKYPEWKFRKAIAAVNQADEEKLSEVLTRMHEEDPTWIIEYSKELKQTIVHGQGEFHL
                     KTLKWRIENNDKILINYSNPRIPYRETITKTARADYRHKKQSGGAGQFGEVHLIIEPC
                     VEGVPEKEVYKINGQEIKISVRDKQEYPLEWGGKLVFINSIVGGVIDARFLPAIQKGI
                     MDCMEQGPLTGSYARDIRVIVYDGKMHPVDSNEISFRLAGKYAFSEAFKNAGPKVLEP
                     IYDVEVLVPSASMGDVMSDLQGRRALIMGMESDKGMEKIFAKVPLKEMNNYSISLSSN
                     TRGRASFSMKFASYELLPSDVQDALLKEYEKSKVAE"
     gene            complement(3046..4614)
                     /locus_tag="PLD76_02120"
     CDS             complement(3046..4614)
                     /locus_tag="PLD76_02120"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOU26834.1"
                     /translation="MGKVKITETALRDAHQSLIATRMRTEDMLPILEKMDNIGYHSLE
                     AWGGATFDSCIRFLNEDPWDRLRKIKQVVKKTPLQMLLRGQNLLGYKHYADDVVEYFV
                     QKAIDNGMDIIRIFDALNDPRNIETAIKACIKEGGHAQACVCYTTSPVHNLELFAKDA
                     KTLENMGAHSICIKDMAGLLKPYDAYELVKAIKETVNIPVQLHTHYTSGVASMTLLKA
                     IEAGVDVVDTAISPMAMGTSQPPTEPLVASLQGHERDTELDLVKLSEIAEYFRPLKEK
                     WLESNMLDTKVMGVDVNALIYQVPGGMLSNLVSQLKQANAMDKYEEVLREVPRVREDF
                     GYPPLVTPSSQIVGTQALFNVLMGERYKMVPQESKGIIKGEYGKTPAPISDEFRKKIL
                     GDEEAITCRPADLIAPELEKTREAIKDLMIQEEDVLTYALLPQVAEKFFQMRKNGTLE
                     AVKEEQKKQATPVVASSKPAQETAESNDDEDEVAAVMSAAIATIESENGGGQFAITSV
                     RQVSPWVISARLNR"
     gene            complement(4627..4989)
                     /locus_tag="PLD76_02125"
     CDS             complement(4627..4989)
                     /locus_tag="PLD76_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU26835.1"
                     /translation="MKKYLITVNQKQYEVEVVEVKQQVQTQKTTSGIKPSVTVSKPKE
                     TVAGANGHKVAAPMPSNIIKLLVNEGDVVNEGDNLLVFEAMKMENNLHSPVSGKIASI
                     KVKVGNSVAAGDTLIIIE"
     gene            complement(5205..>5828)
                     /locus_tag="PLD76_02130"
     CDS             complement(5205..>5828)
                     /locus_tag="PLD76_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU26836.1"
                     /translation="IRGYLEILLGDKPVSEDKRKYFLERSYYQTLRLSDLINDVALIT
                     KMEEAATVFEKEKVNVRELAQEAIEELSFRINPEQVDIQNNLENNLDIVGNKSLIFAI
                     FRNLLENAINYAGEEITIGIKCYNEDADYYYFLFYDTGCGVKPECLEKIFDRFVRIDQ
                     GRSRRNGGTGLGLSIVKHAVMFHNGTIEARNRKTGGLEFYFTLKKRK"
BASE COUNT         1729 a         1008 c         1194 g         1897 t
ORIGIN      
        1 acgtagatgc cgtgcgtgag gcggtaaaag atgaaaatgc tgaaatattg attgttgcgg
       61 ctgctaccga agcagatatt gccgaattag aaacctacga ggagcgtcag atgtttttag
      121 aagaagtggg attatcggag tcgggtgtag cacgattaat taaatcggct tacaaattgc
      181 taaatttaga aacttatttt acagcaggtg tgcaagaagt gagggcttgg acctacgaaa
      241 agggctggaa agcaccacaa tgtgcaggtg ttattcatac cgattttgaa aaaggtttta
      301 ttagagccga agtaattaag tatattgatt ttacaacctt gggatcagaa gctgcttgca
      361 aagaggctgg aaaaatgagc gttgagggaa aagaatatgt tgtacaagat ggagatatta
      421 tgcattttag gtttaatgtg taacaaaata tctgtttaat aaaagaatca aaaaggggga
      481 aacattgttt cccccttttt ttattacccc tataaaaaac aaaaggtagt ctcgcctgac
      541 tacctacttg ctaaccttaa aatcaaatac tatgaaaaaa tcatatgcga atatacgggc
      601 aaaatacaat taactacaaa atattggttt actaagtctt taatttaaca gatttttggc
      661 ttcatattac aaaattgtta caaatattgt tacacaacat attttcacta ttgttttttt
      721 actaactatt tcctacattg caaggcattt tttttgcttt cgagggattc gtttacaaca
      781 atttagtaac cttaatacca ataattgtat gaaagtgtat caaacaaatg aaattaagaa
      841 tatctcactt gtgggatctt ctggctcagg aaaaaccacg ctcactgaag caatgctctt
      901 tgagagtgga gttataaaac gtagaggcag tattgaagca aaaaatacgg tttctgatta
      961 ttttcctgtc gaacatgagt atggttactc tgtttttcca actgtttttc attctgaatg
     1021 gatgggcaga aagctgaata tgatagattg tccaggtgct gacgactttg tgggcggttt
     1081 gattacttcg cttagtgtta ccgacactgc gctaatgctt attaatgcac aacatggtgt
     1141 agaagtaggt acgcaaaatc aatacaggca aatagagaaa cttaaaaaac catgcatatt
     1201 tgtcgtgaat caacttgacc atgaaaaagc agattttgat aaaacagtgg atgatttgcg
     1261 tcaaaatttt ggcaataaag tggtacttat tcaatatccg attcaaacgg gacctgcttt
     1321 taatgccgtt gtagatgtat tgctaatgaa gttgtatacg tggaaagctg atggtggcgt
     1381 accaacgatt agcgatattc cagccaatga acttcaacga gcttccgaat tgcacaatat
     1441 tttagtggag gcagctgcag aacatgagga aaatttgatg gaaaaatatt ttgccgatgg
     1501 taatttgact gaagatgaaa tgcgtatggg tattcgtttt ggcttggtgc atcgtgatat
     1561 ttttcctgtt ttttgcatta gtgctgccaa ggatatgggc gtgcgtcgtt tgatggaatt
     1621 tttagggaat gtagttccct atgttaggga ttcggcagct cctgtagcaa ctaagggaga
     1681 agaggttgct ccattacaag aaaataacgc ttctatttat gtgtttaaaa cctctgtaga
     1741 gccgcatatt ggcgaggtct cattctttaa ggtgatgtct ggaaaagtaa aagaaggcga
     1801 tgatttgtat aatgccactc gccaaagcaa agagcgatta actcaaatct acgctgtgtg
     1861 tggaagtatt agaacaaaag tggatgaatt agtagcaggg gatattggtg ccacagttaa
     1921 gctcaaaaat acgcgtacat ccaatacctt aaacggaaaa aatgctgact tacagtttga
     1981 ttttattaaa tatcccgaat ggaaattccg taaagcaatt gcagcggtta atcaggctga
     2041 tgaggaaaaa ctaagtgagg tgttaactcg catgcacgag gaagacccaa cttggataat
     2101 tgaatactct aaagaattaa aacaaacgat tgttcatgga caaggagaat ttcatctcaa
     2161 aacacttaaa tggcgtatcg aaaataacga taaaatcttg attaattatt caaatccacg
     2221 tattccatac agagaaacca ttacgaaaac agctcgagcg gattaccgac ataaaaaaca
     2281 gtcgggtggt gcgggtcagt ttggcgaggt acatttgatt attgaaccat gtgtagaagg
     2341 tgttcctgaa aaagaagtgt ataagattaa tggacaggaa ataaaaattt ctgtacgtga
     2401 taaacaagaa tatccactag agtggggagg gaagcttgtg tttattaaca gtattgtagg
     2461 tggtgtgatt gatgctcgat ttttgcctgc tattcaaaaa ggaataatgg attgtatgga
     2521 gcaaggtccg ctaacaggtt cgtatgcacg tgatatccgt gtaattgtgt atgatggaaa
     2581 aatgcaccca gtagattcta acgaaatatc tttccgtttg gcgggtaaat atgcttttag
     2641 cgaggctttt aaaaatgcag gaccaaaagt attagagcct atatacgatg tagaagtgct
     2701 agtgcctagt gcaagtatgg gcgatgtaat gagcgatttg caaggacgtc gtgcgttgat
     2761 aatgggaatg gaatcagaca aaggtatgga aaaaatattt gcgaaagttc cattaaaaga
     2821 aatgaacaat tattccattt cgttgagttc taatactcgg ggaagagctt cttttagcat
     2881 gaaatttgct tcttacgaat tactgccttc tgatgtgcaa gatgccttgc ttaaagagta
     2941 cgaaaagagc aaggtggctg aataaataat gatttagttt ttgaacaaaa ataaggcgtt
     3001 tcagaatttc tgaaacgcct tatttactat ctgtcatgta aaatgctatc tatttagtcg
     3061 agcggaaata acccaagggc ttacttggcg aacagaagtt attgcaaact gacctccacc
     3121 attttcgctt tcgatagtag ctatggcggc agacattact gcagctactt catcttcgtc
     3181 atcgttgctt tctgctgtct cttgtgcagg ttttgaagat gcgacaacag gagttgcttg
     3241 ttttttttgt tcttctttta cggcttcaag cgtaccattt ttacgcattt ggaagaattt
     3301 ttctgctact tgtggcagta aggcatatgt tagtacatct tcctcttgta tcatcaaatc
     3361 tttaatagct tcgcgtgttt tttccaattc tggagctatc aaatcagctg gtcggcaggt
     3421 aatggcttct tcatctccta gaattttttt tctaaattcg tctgatattg gagctggagt
     3481 tttaccatat tcgcctttaa taataccttt tgactcttgt ggcaccattt tgtatcgttc
     3541 gcccatcaat acgttgaaaa gagcttgcgt acccacaatt tggctcgatg gagtaactag
     3601 cggaggatag ccgaaatctt cgcgtacacg aggtacttca cgaagcactt cttcgtattt
     3661 atccatagcg ttggcttgtt taagttgcga aactaagttc gacaacatac cgccaggtac
     3721 ttgataaata agagcgttta catctactcc cattactttg gtatccaaca tgtttgattc
     3781 gagccatttt tctttcaacg gacggaaata ttcggcaatt tcgctcagtt ttaccaaatc
     3841 taactcagta tccctttcat gtccttgcaa tgaggcaaca agcggttcgg taggaggttg
     3901 agacgtaccc atcgccatag gcgaaatggc tgtgtccacc acatcaacgc ctgcttctat
     3961 cgcttttagc aaggtcatag aggctacacc actggtgtaa tgcgtatgta attgaacagg
     4021 aatgttaacg gtttctttta ttgcttttac gagttcgtac gcatcgtatg gtttcaacaa
     4081 gccagccata tctttgatac agatggaatg ggctcccatg ttttcgagcg ttttagcgtc
     4141 tttagcaaat agttctaaat tgtgcacagg gctagtggta taacaaacac acgcttgtgc
     4201 atgaccacct tctttgatac atgctttaat agcggtttca atattgcgag ggtcgttaag
     4261 tgcatcaaat atgcggatga tatccatacc attatcaatc gctttttgca caaaatattc
     4321 cactacatca tcagcataat gtttgtaacc taggaggttt tgacctctaa gtaacatttg
     4381 taaaggagtc ttttttacaa cttgtttaat tttccgtagc ctatcccaag ggtcttcgtt
     4441 taggaaacgg atacaagaat cgaatgtagc accaccccaa gcttcgagcg agtggtagcc
     4501 gatattatcc attttttcaa gaatggggag catatcttct gtacgcatac gtgtagctat
     4561 cagcgattga tgtgcatcac gtaatgctgt ttctgtaatt ttaacttttc ccattgttgt
     4621 agtgtcttat tctatgatga ttaatgtgtc tcccgcagct acagaattgc caacttttac
     4681 tttgatagag gcaatttttc cgcttactgg agagtgtagg ttgttttcca ttttcatggc
     4741 ttcaaatacc aatagattat ctccttcgtt tactacgtct ccttcgttta ccaacaattt
     4801 aattatattg ctaggcatgg gtgccgcaac tttatgacca tttgctcccg ccaccgtttc
     4861 tttaggtttt gatacagtta cactaggttt gatgccagaa gttgtttttt gcgtttgtac
     4921 ttgttgtttt acttccacaa cttctacttc gtattgtttt tggttaacgg taatgagata
     4981 ctttttcatg aataaattgt ctttaataat gttttaattg gtatgtatgt ttttattctc
     5041 agtaggttat ctcgtgaaga gataatttac tatgcaaaag tacagctttt ttagtaatct
     5101 gttttacttt tagaaaatat taacgaccaa aacgctcttt tttaccgaat tacatagttt
     5161 tcagtagaaa agagcgtttc tattttataa gaatgataca atatttattt tcttttttta
     5221 agagtaaaat aaaattctag tcctcctgtt tttctatttc gggcttcgat agttccatta
     5281 tgaaacataa cagcgtgttt tacaatggat agtcctagtc ctgtaccacc attgcgtcta
     5341 ctccgtcctt ggtcgatacg tacaaagcga tcgaatattt tttctagaca ttcaggtttt
     5401 acgccacaac cggtatcata aaacaagaaa taatagtagt cagcgtcttc gttgtaacac
     5461 ttgatgccga tggtaatttc ttcgccagca tagttgatgg cattttcgag taaattacga
     5521 aatatggcaa aaataaggct tttgtttcca acgatatcaa ggttattttc caagttgttt
     5581 tgaatgtcaa cttgttctgg attaatacga aaactcaatt cttcgatagc ttcttgtgct
     5641 agttctctta cgtttacttt ttctttttca aatacggttg cagcttcttc catttttgta
     5701 attaaagcaa catcgttaat taaatcagaa aggcgaagag tttggtaata cgaacgttca
     5761 agaaaatatt ttcgtttgtc ctcagataca ggtttgtctc caagcaaaat ttcaagatat
     5821 ccacggat
//
LOCUS       DAOSMY010000054         5787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14005_length_5787_cov_3.667481, whole genome shotgun sequence.
ACCESSION   DAOSMY010000054 DAOSMY010000000
VERSION     DAOSMY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14005_length_5787_cov_3.667481"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLD76_02135"
     CDS             complement(<1..220)
                     /locus_tag="PLD76_02135"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOU26837.1"
                     /translation="MRKNLFIVFIISIIIPISTFARVKNVIFLIGDGMGLNQIHASSI
                     VADSALNMQRAHYIGLVDTHSADHYITDS"
     gene            complement(236..598)
                     /locus_tag="PLD76_02140"
     CDS             complement(236..598)
                     /locus_tag="PLD76_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU26838.1"
                     /translation="MEEATNNIQESKQKLYFSIKEVAELVDVNISAIRFWEQQFDKIK
                     PRKNDKGTSFYTREHIDEFLLIKHLKEEKGLTLDGIKKRLKENKASTKKNFEIIDTLQ
                     KIKKQLHNIKTELDDLIE"
     gene            complement(607..1581)
                     /locus_tag="PLD76_02145"
     CDS             complement(607..1581)
                     /locus_tag="PLD76_02145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU26839.1"
                     /translation="MGKAKYHFNEHTLNYDRIDNTIRHRLIKIGQYLIGSILMGIVFF
                     VIFIFTVKSPNEKLLEAENKRISTQYELLSRQLDDAMEVLTNIQQRDDNFYRVVLQVD
                     SIPSSIRRRNYINSNRYQKLLRMRTSDLVIATSQKMDMVSRMLYIQSNSFDELVALAK
                     QEEKKLQHIPAIQPIPNKDLKRTASGYGWRIDPIYHTRRFHKGMDFSAPTGTKIFATG
                     NGTVIKAGWEQGYGNAVFIDHGFGYVTVYAHMHQLKKGLRRGSKVSRGEVIGFVGNTG
                     KSTGPHLHYEVRLKGKPQNPQNYYFMDLSPEEYDQMIQISANYGRVFD"
     gene            1813..4434
                     /gene="alaS"
                     /locus_tag="PLD76_02150"
     CDS             1813..4434
                     /gene="alaS"
                     /locus_tag="PLD76_02150"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU26840.1"
                     /translation="MKSAKEIRESFTSFFASKGHKIVPSAPMVIKNDPTLMFTNAGMN
                     QFKDIILGNATVVYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KKDAIDWAWEYLVEVLKINPEILYVTVFEGNPAEGLERDNEAAAYWAQYLPQDRIING
                     SQKDNFWEMGDTGPCGPCSEIHVDLRSAEEKLKVPGVKLVNESHPQVIEIWNLVFMQF
                     NRKADGSLELLPAKHVDTGMGFERLCMAIQGKTSNYDTDVFQPIIKAMATLAGVSYGE
                     NNEQDIALRVIADHIRTIAFSITDGQLPSNAKAGYVIRRILRRAVRYGYTFLNQRSSF
                     MHALLPALIDSMGSAYPELEAQKGLIEKVIKEEEESFLRTLETGIRLLDKVITEAKLA
                     GKTTISGKDAFVLYDTYGFPLDLTELIAKENNVMVDVAEFEQEMQKQRERARNAASVE
                     TGDWIVVRDGESVFDGYDFAEMDVQLLRYRKMVQKGKYLYQLVFDRTPFYGEMGGQVG
                     DTGYILADTKKIAIVDTKKENGLTVHITLELPETIDATYTAVVNVEKRKRIAANHTAT
                     HLLHEALREVLGTHVEQKGSFVSDEALRFDFSHYQKVSAEELKTVELIVNKKIRNNAS
                     LQEYRELPVDEANKMGAMALFGEKYGEKVRVIQFGTSVELCGGSHVQSTGEIGMIKLV
                     HESSVAAGIRRIEAYTADKAEAYITAQLDVLTEVKQLFNNPTNVVGVITKFQQDTLQL
                     KKQLDGYVQEYVKQTKATLLTQVQQINGVDIIVFKSHDSAEICKDIAFQFKSQFANIP
                     FAFVAATQNDDKVSVSLLLSDNLVKKGLHASTIIKSIASDIDGSGGGQPFYATAGGKR
                     ASGIDTAVKKIIESITA"
     gene            complement(4525..4875)
                     /gene="rplT"
                     /locus_tag="PLD76_02155"
     CDS             complement(4525..4875)
                     /gene="rplT"
                     /locus_tag="PLD76_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU26841.1"
                     /translation="MPRSVNHVASRARRKKILKLTRGYYGARKNVWTVAKNTWEKGLQ
                     YAYRDRKTKKRNFRALWIQRINAAARLEGMSYSKFMGALHKANIDINRKVLADLAMNQ
                     PEAFKAIVKKVQKA"
     gene            complement(4981..5178)
                     /gene="rpmI"
                     /locus_tag="PLD76_02160"
     CDS             complement(4981..5178)
                     /gene="rpmI"
                     /locus_tag="PLD76_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOU26842.1"
                     /translation="MPKMKTNSGAKKRFALTGSGKIKRKHAFKRHILTKKTTKAKRAL
                     TSFGLVDKTDESNVKFLLALK"
     gene            complement(5206..5772)
                     /gene="infC"
                     /locus_tag="PLD76_02165"
     CDS             complement(5206..5772)
                     /gene="infC"
                     /locus_tag="PLD76_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU26843.1"
                     /translation="MNKAIIAPKVRLVDPEGKLDAAINAAIIEGKLDPAILRGSEITG
                     IMFPKRALEFAEALDLDLVEVSPNAEPPVCRVVDYQKFLYQRKKREKEIKAKTVKMVV
                     KEIRFGPQTDEHDYNFKLKHAIKFLEDGDKLKAFVFFKGRSILFKEQGEVLLLRFEKN
                     LEEYAKVDQMPLLEGKKMIIMLSPKKKK"
BASE COUNT         1810 a          952 c         1090 g         1935 t
ORIGIN      
        1 ccgaatcagt aatgtaatga tcagccgaat gggtgtctac aagtccaata taatgggctc
       61 gttgcatatt taacgcactg tcggcaacta tgctagaggc atgaatttga tttaaaccca
      121 taccatctcc aattaagaaa atcacatttt ttaccctagc aaacgtgcta attgggatga
      181 taatgctgat aataaataca ataaataggt tttttctcat ggtttttgtt tgtttttatt
      241 caattaagtc atcaagctca gtttttatat tgtgtagttg tttttttatt ttttgtaggg
      301 tatcaataat ttcaaaattt tttttagttg atgctttgtt ctcttttaag cgtttcttta
      361 ttccatctaa tgttaatcct ttttcttctt ttaaatgttt gatgagtaag aattcgtcaa
      421 tgtgttctcg cgtataaaaa cttgttcctt tatcgttttt tcgaggctta atcttatcaa
      481 attgctgttc ccagaaacgt atagcagaaa tattaacatc cactaattca gctacttctt
      541 ttatggagaa gtatagtttt tgtttgcttt cttgtatgtt gttagtagct tcttccatta
      601 gtagagttaa tcaaatacac gtccataatt agcagatatt tgtatcattt gatcatattc
      661 ttctggagat aaatccataa aataatagtt ttgaggattt tgaggttttc ctttcaagcg
      721 tacctcataa tgtaggtgag gacctgttga tttacctgta tttcctacaa atccaattac
      781 ttcacctcta cttaccttac tgcctctacg tagccctttt ttgagttggt gcatatgtgc
      841 ataaacagta acatagccaa aaccatggtc gataaataca gcatttccat atccttgttc
      901 ccatccagct ttgataacag taccattgcc agtagcaaaa atttttgtac ctgttggtgc
      961 cgaaaaatcc attcctttat gaaatctacg tgtatgataa attgggtcaa ttcgccatcc
     1021 atagccagaa gctgtccttt ttaaatcctt gttaggaatg ggttgtatag ctggaatatg
     1081 ttgtagcttt ttttcttctt gtttagctaa tgctacaagt tcgtcaaaag aattagattg
     1141 aatatataac attcttgata ccatatccat tttctgactg gtagcaatga ctaaatcaga
     1201 cgttcgcatt ctcagaagtt tttggtaccg attgctgttg atataatttc tacgtcggat
     1261 actcgaagga atagaatcca cttggagtac tactcgataa aaattgtcat cacgttgttg
     1321 aatatttgtt agcacttcca tagcatcatc taattgccta gaaagtaact cgtattgtgt
     1381 gctaatacgt ttattttcag cttctagtaa cttttcatta ggagatttga ccgtaaaaat
     1441 gaagataaca aaaaaaacga tgcccattaa aatacttccg attaaatatt gtccaatttt
     1501 aatcagacgg tgcctaattg tattgtctat tcgatcgtag ttaagggtgt gttcgttaaa
     1561 atgatatttt gcttttccca tgattgttgt ttcctaaaac caatctgttt attacacagg
     1621 acaaacagat taacaaaagt agtttgaaaa ttcatttaag acaacaaaaa taacaaaaaa
     1681 aatactaatt ttgtggcttg aaatctgaat ttgcaaatta ttttagatac caatatctaa
     1741 ctgcttggta taatttagtt tgtatactag atacgtatgt ttgatttgat aaattttaat
     1801 tgaaaaataa taatgaaatc tgccaaagaa attagagaat cgtttacatc cttttttgca
     1861 tccaaaggac ataagattgt tccgtcagca cccatggtaa ttaagaatga tccaacactc
     1921 atgtttacaa atgcggggat gaaccaattt aaagatatta ttttgggaaa tgctacagtc
     1981 gtatatcctc gtgtagcaaa ttcgcaaaaa tgtttgcgtg taagtggcaa gcacaacgat
     2041 ttggaagagg tagggcacga tacttaccac cacaccatgt ttgaaatgtt gggtaattgg
     2101 agttttggcg attactttaa gaaggatgca attgattggg catgggagta cctagtagag
     2161 gtgttaaaaa ttaatccaga aatattgtac gttactgttt ttgagggcaa tccagccgaa
     2221 ggcttagaaa gagataacga ggcagcagca tattgggcac aatatttacc ccaagaccgt
     2281 attattaatg gtagtcaaaa agataatttt tgggaaatgg gagatacagg tccatgtggt
     2341 ccatgttccg aaatacatgt tgatttacgt tcagcagaag aaaaactaaa agtacctggt
     2401 gtaaaattag taaatgaaag tcatccacaa gtgatagaaa tttggaatct tgtgtttatg
     2461 cagtttaatc gcaaagcaga cggttcgttg gaattattac ctgccaaaca cgtagatact
     2521 ggcatgggat ttgaacgttt gtgtatggca atacaaggaa aaacatcgaa ttacgatacg
     2581 gatgtatttc aacctataat taaagcaatg gctacactag ctggggtttc ttatggggaa
     2641 aataacgaac aagatattgc attgcgtgta atagccgacc atattcgtac gattgctttt
     2701 tcgattaccg atggtcagtt gccttctaat gcaaaagcag ggtatgttat tcgtcgtatt
     2761 ttgcgtagag cggtgcgata tggatatacg tttttaaatc aacgttcatc gtttatgcat
     2821 gcattacttc cagcacttat tgatagtatg ggaagtgctt atccagaatt agaagcacaa
     2881 aaagggttga tagagaaagt gattaaagag gaagaagaat catttttacg taccttagaa
     2941 acaggtatcc gtttgctcga taaagtaatt actgaagcca aacttgcagg taaaacaacc
     3001 ataagtggta aagatgcttt tgtattgtac gatacgtatg gttttcctct tgatttaact
     3061 gaattgatag ccaaggaaaa taacgtaatg gtagatgtag cagagtttga acaagaaatg
     3121 caaaaacaac gcgaaagagc cagaaatgca gccagtgtag aaacgggtga ttggattgta
     3181 gttcgcgatg gagaatcagt atttgatggg tatgattttg ctgaaatgga tgtacaattg
     3241 cttcgttacc gtaaaatggt acaaaaggga aaatatctat accaattagt tttcgatcga
     3301 acaccgtttt atggagaaat gggcggtcaa gtaggagata ctggctatat tcttgcagat
     3361 actaaaaaaa ttgccattgt tgatactaaa aaggaaaatg ggttaacggt acatattaca
     3421 ttagaattac cagaaactat tgatgctacc tatacagcag tggtaaatgt ggaaaaacgc
     3481 aaaagaattg cggctaacca tacagcaacc catttacttc acgaggcatt acgcgaggta
     3541 ttgggtacac atgtagaaca aaaaggttcg tttgtgtcgg atgaagcatt gcgttttgat
     3601 ttttcgcatt accaaaaagt aagtgccgaa gaattaaaaa cagtagaatt gatcgtaaat
     3661 aaaaagattc gcaataatgc atctctgcaa gaatatagag aattacctgt tgatgaagca
     3721 aacaaaatgg gagcaatggc attgtttggc gaaaaatatg gcgaaaaagt acgtgttatc
     3781 cagtttggca cttcggtaga attatgcggt ggttcgcacg tacaatcaac tggagaaatc
     3841 ggaatgatta aacttgtgca cgaaagttcg gtagcagcag gtattcgtcg tatagaagcc
     3901 tatacagcag acaaagcaga agcgtatatt acagctcaat tagatgtact tacagaagtt
     3961 aaacagttat ttaataaccc tacaaatgtg gtaggtgtaa tcacaaaatt tcagcaagac
     4021 acacttcaat taaaaaaaca gttggatggc tatgtgcaag agtatgtaaa acagactaaa
     4081 gcaacgttat taactcaggt acagcaaatt aatggcgtgg atattattgt gtttaagtcg
     4141 catgattcgg cagaaatttg caaagatata gcctttcagt ttaaatcaca gtttgcaaac
     4201 atcccatttg cttttgtagc agcgacacaa aacgatgaca aggtaagtgt atcgttgttg
     4261 ttatcagata atttagtaaa aaaaggatta catgcttcta caatcattaa atcgatagca
     4321 tcagatattg atggaagtgg aggagggcaa ccattttacg caacggcagg aggaaaaagg
     4381 gcttcgggta tagatacggc tgtaaaaaag attattgaat cgataactgc ttagtaggag
     4441 taaacgatat aactaaaaag aggaacaatt tattttgttc ctctttttag ttatataaag
     4501 tagtaaaact ttattctaat tacactacgc tttttgcact ttttttacaa tagctttgaa
     4561 tgcttctggt tggttcatag ctaaatcggc aagtaccttg cggttgatgt cgatgtttgc
     4621 tttgtgtaga gctcccataa atttagaata agacattcct tctaatctag cagctgcatt
     4681 tatacgttga atccataatg cacggaagtt gcgttttttg gttttacggt cacgatacgc
     4741 atactgtaac cctttttccc aggtattttt ggctactgtc catacgtttt ttctagcacc
     4801 gtagtaacct ctggttagtt ttaagatttt ttttcttctt gctctcgaag caacatgatt
     4861 gactgatctt ggcattttct tctgtttttt tgaatggtag cgtcactaat aggtgatctt
     4921 tcggctattc attcggttaa tactgaattg atttacaaat aaaataaact ttgacgtaga
     4981 ctatttaagt gctaacaaaa atttaacatt gctttcgtct gttttgtcaa caagtccgaa
     5041 actagttaag gctctttttg ctttggtagt tttcttcgtc aagatgtgcc ttttaaatgc
     5101 atgttttctc ttgatttttc ctgatccggt aagggcaaac ctctttttgg caccggagtt
     5161 agttttcatt tttggcatta cgttgtatat ttaaaaatta aaaaattatt ttttcttttt
     5221 gggcgaaagc ataatgatca ttttttttcc ttctaataaa ggcatttgat ccacttttgc
     5281 gtattcttct aagttttttt caaaacgtag tagcaagact tctccttgtt ctttaaataa
     5341 aattgaacga cctttaaaaa atacaaaagc ctttagttta tcaccatctt ctaaaaattt
     5401 aatagcatgc ttcaatttaa aattataatc atgttcgtca gtctgtggac cgaaacgaat
     5461 ctctttcacc accattttta cagttttagc tttgatctct ttttctctct tcttacgttg
     5521 atagaggaat ttttgataat caacaacacg acatacaggc ggttctgcat taggtgatac
     5581 ttccactaag tctagatcca gcgcttctgc aaactccaat gctcttttag ggaacataat
     5641 gcctgttatt tcactgcctc gtaagatggc aggatccaat ttcccctcaa ttatcgctgc
     5701 attgatagct gcgtcaagtt tcccctccgg atcaactaat cgcactttag gtgcaataat
     5761 agctttattc acccgaaccg tctgttt
//
LOCUS       DAOSMY010000055         5783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14017_length_5783_cov_4.300978, whole genome shotgun sequence.
ACCESSION   DAOSMY010000055 DAOSMY010000000
VERSION     DAOSMY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14017_length_5783_cov_4.300978"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLD76_02170"
     CDS             <1..461
                     /locus_tag="PLD76_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOU26844.1"
                     /translation="GKDPNRYRPSAEALCRRLVKGESLYRISTIVDCINLISVKTGYS
                     IGGFDRDSIIGNSLELGIGQAGEEFYAIGRGLLNIEGLPVYRDEKGPIGTPTSDHERT
                     KISLNTHHLVLIVNGFDGSITSVQQTLDFSRELLLKYTNASNISEKIVPA"
     gene            complement(463..933)
                     /locus_tag="PLD76_02175"
     CDS             complement(463..933)
                     /locus_tag="PLD76_02175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU26845.1"
                     /translation="MKKIQNPWTILDGYNCFACSPANEIGIKMEFFEEGEELICKWEP
                     SKHYEGFTNVLHGGIQATLLDEMGAWVVARKLQTVGFTSKLTIKYHKTVVINQPYIYI
                     KAKLTEVKRNLAFIEAHIYNYEEQLCASAEMCYFCTSQEAASSSFHFNGCSLEE"
     gene            complement(952..2418)
                     /gene="cysS"
                     /locus_tag="PLD76_02180"
     CDS             complement(952..2418)
                     /gene="cysS"
                     /locus_tag="PLD76_02180"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU26846.1"
                     /translation="MEHTLQIYNTLTRKKEVFIPIHSPNVGLYVCGPTVYGDAHLGHA
                     RPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEHDADEGEDKVAKKARVEQKEPMEIV
                     QFYLNRYRKAMEQLNVLPPSIEPHASGHIMEQIEFTQKIIDAGFAYQSEGSVYFDVEK
                     YNKQYHYGKLSGRNIEELLETTRTLDGQSEKKKSVDFALWKKASPEHIMRWKSPWSDG
                     FPGWHMECSAMGTKYLGEEFDIHGGGMDLVFPHHECEIAQSTAALGKETVRYWMHNNM
                     ITINGQKMGKSLGNFITLDEFFGGSHPLLSQAFSAMTIRFFILQAHYRSTVDFSNEAL
                     QASEKGLNKLLETYARLQKITPSATSTIDVSGLRAKCEEAMKDDLNTPIVLSHLFEAS
                     KAINTVADGKASLSAKDVAELKSVFKLFVEDILGLQQTTKDNTQHEAYQKAIDLLLEL
                     RLEAKRNKDFALSDKIRNELTTLGFSIKDTKEGFEWSL"
     gene            complement(2505..2888)
                     /locus_tag="PLD76_02185"
     CDS             complement(2505..2888)
                     /locus_tag="PLD76_02185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU26847.1"
                     /translation="MEEKEHIHYDQFAKNSGIKLVEAKNGEAIAKMEIRNMHLNGLNL
                     VHGGALFTLADFTLGAAVNSLGGAHVTMSASIHYVKSAKEGTLIGISKQVSAGRTIAT
                     YDVEIRLEETNDLIAIVRGNSFKKS"
     gene            complement(2968..4335)
                     /locus_tag="PLD76_02190"
     CDS             complement(2968..4335)
                     /locus_tag="PLD76_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU26848.1"
                     /translation="MKKLFTFLIVSVLTITSLSAADYTHWSVYIDGGASQFDGDVSQR
                     YNQIKPGADIHWTAGLGVEYTFTPFWGLGVEYQYMPYSGYSTSTSQGWWSNYGEFDFE
                     GGSHQAHIFTSLNLTNLLNVYRKTWRFNVYANIGLGASFYNVSQSNIVPASIPPGGWT
                     FARTPNEIEDGRSLLVPMSLNFEYNISKPLAVGLNVHYRATNKDNYEGEAYVRGVQND
                     NFLLGTASLRYKFASGQKDGHIRNITADKFRQEETKEREILDKLDSLNNKVDDLDSVV
                     TNDLTPRMNEVERLLSDKPDADGDGVPDWRDREPNTPAGSFVNYWGESLPYEVLKATE
                     FYEELAKMQEEEVDYDMSVYFAFDKKNLTKRSIDNIAKAAQKLKSNPLLKVELRGYAD
                     YPGSHNYNLRLSTSRGEVVKQELVNKHGIDPSRITLNPQGRMTNPPNANQKNRRTDFV
                     FSKMK"
     gene            complement(4441..5769)
                     /gene="miaB"
                     /locus_tag="PLD76_02195"
     CDS             complement(4441..5769)
                     /gene="miaB"
                     /locus_tag="PLD76_02195"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU26849.1"
                     /translation="MKNQKKLFIETYGSQMNVADSEVVASIMGMSGYELTDNINEANA
                     IFINTCSIRDNAEQKAFSRLDYFQSLKKKRKDIIIGVLGCMAERIKEDLLERKVIDMV
                     VGPDAYLDLPNLIGAVESGEKAINIELSTTETYQDIIPQRIGHNRISGFVSIMRGCNN
                     FCSYCIVPYTRGRERSRPATSIINEMKDLVAKGYKEITFLGQNVNSYLDSSEDNKYTF
                     PDLLAMAANTFPDTRIRFTTSHPKDISEELIRTIATYHNLCKHIHLPVQSGSNRILSL
                     MNRKYTREEYLQKIAMIKRIIPECGITTDVFTGFHSETEADHQETLSLMQEVGFDNAF
                     MFKYSERSGTYAAKHLEDNVPEAIKLSRLQEVVDLQNKLSKESNQKDLGKIVEVLIEG
                     YSKKSRNQLFGKSSENKVVVFNKENKKIGEYAEIRIKDATVATLLGEICE"
BASE COUNT         1625 a         1110 c          979 g         2069 t
ORIGIN      
        1 caggaaaaga tccgaataga tatagaccct cagccgaagc tctttgtcgc agattagtga
       61 aaggagaatc attgtatcgc atttcaacca tagtggattg tattaattta atctcggtta
      121 aaacgggcta ttccatcggt ggattcgata gagatagcat tattgggaat tctcttgaac
      181 ttggcattgg acaagctggt gaagaatttt atgcaatagg aagagggttg ttaaatatcg
      241 aaggattacc cgtgtatcga gatgaaaaag gaccaatcgg aactcctact agcgatcacg
      301 aacgtacaaa aatttctctc aatacacacc atcttgtgct aatagtaaat ggttttgatg
      361 gctcaattac ttctgtgcaa caaacactcg atttttccag agaattgctt cttaagtaca
      421 caaatgctag caatatatca gaaaaaatag taccggcata gcttactcct ctaacgaaca
      481 tccgttaaag tgaaaagaag aagatgctgc ttcttgtgag gtgcagaaat aacacatttc
      541 tgctgaggca cacaattgct cctcgtaatt ataaatatga gcttcaataa atgctaaatt
      601 gcgttttacc tctgttagtt tggctttgat ataaatataa ggctggttaa taacgactgt
      661 tttatggtat ttgatagtta atttactcgt aaaaccaacc gtttgtaatt tgcgagctac
      721 cacccaagct cccatttcat ctaacaaggt ggcttgtata cctccatgca atacgttggt
      781 aaatccttca tagtgttttg aaggctccca tttgcaaatc aactcttctc cttcttcaaa
      841 aaattccatt tttattccta tctcatttgc tgggctacaa gcaaagcaat tgtagccatc
      901 tagaattgtc catggatttt gtatcttttt catgttacaa ggtttttatt cttatagcga
      961 ccattcaaat ccttcttttg tatctttgat gctaaaacca agagttgtta attcgtttct
     1021 aattttatct gatagggcaa aatctttgtt tcttttggct tctaatcgta actcaagcaa
     1081 caaatcaata gctttttggt atgcttcatg ttgagtattg tctttggtcg tttgttgtaa
     1141 tcctaatatg tcttcaacaa ataacttgaa tactgatttt agttctgcta catcctttgc
     1201 acttaagctg gcttttccat ctgccacagt gttaatggct tttgaggctt caaaaagatg
     1261 tgataatact atcggtgtat ttagatcatc tttcatagcc tcttcgcatt tagctcgcaa
     1321 accagataca tcgatagtag atgtggctga aggagttatt ttttgcaagc gtgcatacgt
     1381 ttcaagcaac ttgttaagtc ctttttcaga agcttgtaat gcttcgttgc taaaatctac
     1441 ggtgctacga taatgtgctt gaagaataaa aaagcgaatg gtcatggctg aaaatgcttg
     1501 tgaaagcagt ggatgcgaac ctccaaaaaa ctcatctaat gtaataaaat tacctagcga
     1561 tttgcccatt ttctgcccat taatagtaat catattgttg tgcatccagt aacgtacagt
     1621 ttctttgcct aaagcggctg tcgattgtgc aatttcgcac tcatgatgag gaaaaactaa
     1681 atccatacct ccgccatgta tgtcaaattc ttctcctaag tatttcgtgc ccatagccga
     1741 acactccatg tgccagcccg gaaaaccatc gctccatggc gatttccatc gcataatatg
     1801 ttctggcgat gcttttttcc acaaggcaaa atccacactt ttcttttttt cactttgccc
     1861 gtcaagtgtt cgtgttgttt caagaagttc ttctatattt cttcctgaca attttccgta
     1921 gtggtactgt ttgttgtatt tttctacatc aaaatatacc gaaccctcac tttggtaggc
     1981 aaaacctgca tcaataattt tttgtgtaaa ttcaatttgc tccataatgt gtccagacgc
     2041 atgtggctca atacttggtg gcaatacatt caattgctcc atggctttgc ggtatctatt
     2101 taggtagaat tgaacaatct ccatgggttc tttttgttct actctagctt tttttgcaac
     2161 cttatcttct ccttcatctg cgtcatgctc aagatgtcct acgtctgtta tgttgcgtac
     2221 ataacgcacc ttatagccta aatgggttaa gtaacgaaac agtaaatcaa aagtaatagc
     2281 gggtcgtgca tgacctaaat gagcatcgcc atatacagtt ggtccacata catacaagcc
     2341 cacatttggg ctatgaatgg gtataaaaac ttctttcttt cgagttagtg tgttataaat
     2401 ttgcaatgta tgttccataa attcaactgt ttgttttctt tcctgcaaaa ttacatattt
     2461 tattctacta taattatgct tttctacaag aattcttcat atttctaaga tttcttaaac
     2521 gaatttcctc taacgattgc aattaagtca tttgtttctt ctaatcgtat ttctacatcg
     2581 tatgttgcga tagttctacc tgcagaaacc tgtttagata ttccaataag tgttccttcc
     2641 ttggcagatt ttacatagtg aatacttgca ctcatcgtta catgagctcc tccaagagag
     2701 ttgacggctg cccctaaagt gaaatctgct aaggtaaata aggctccacc atgcaccaga
     2761 ttcaagccgt ttagatgcat gtttcttatt tccatttttg cgattgcttc tccatttttt
     2821 gcttcgacta atttaatacc cgagtttttt gcaaattggt cgtaatgaat atgttctttt
     2881 tcttccataa tatgttgtta ttgttaaaaa agccctgacg tcatgttgac tcagggcttt
     2941 ttctattaat taaagactac aatctttcta tttcattttg ctgaacacaa agtcagttct
     3001 tctgttcttt tggttagcat ttggtggatt ggtcattctt ccttgtggat tcaaggtgat
     3061 tcttgaagga tcaataccat gtttgtttac taactcttgc ttaacaactt caccacgaga
     3121 agtactcaac cgtaagttgt agttgtggct accagggtag tcagcatatc ctctaagttc
     3181 tactttcaat aatgggtttg attttaattt ttgagccgct tttgcaatat tgtcgataga
     3241 acgttttgtc aagttctttt tatcaaatgc aaagtaaaca gacatgtcgt aatcaacttc
     3301 ttcctcttgc attttagcta attcttcgta aaactcagta gctttcaata cttcataagg
     3361 aaggctttct ccccaatagt ttacaaacga acctgcaggt gtatttggtt ctctatctct
     3421 ccaatctgga actccgtcgc catcagcatc tggtttgtca gaaagtaatc tttctacctc
     3481 gttcatgcgt ggagttaaat catttgtaac aacactatca agatcatcta ccttgttgtt
     3541 taatgaatca agcttgtcaa gaatttctct ctctttagtt tcttcttgtc taaatttatc
     3601 agcagttatg tttctgatat gaccatcttt ttgtccagaa gcaaatttat aacgtaaact
     3661 agcagttccc aataagaaat tatcgttttg aacgcctcta acgtaggctt ctccttcata
     3721 attatctttg tttgttgctc tatagtgaac atttaaaccc actgctaatg gtttagaaat
     3781 gttgtattca aagttcaaag acataggaac taacaacgaa cgaccatctt caatttcatt
     3841 tggagttctt gcaaaagtcc aaccacctgg agggatacta gctggtacta tattggattg
     3901 agacacatta tagaaagaag cacctaaacc aatgtttgca tagacattaa acctccatgt
     3961 ttttctatac acatttaata aattagttag atttaaagaa gtaaatatat gtgcttgatg
     4021 cgatcctcct tcaaagtcaa attctccgta attagaccac catccttgac ttgttgaagt
     4081 tgagtaacca gaataaggca tatattggta ttctacgccc aatccccaaa atggagtaaa
     4141 tgtatattca actcctaatc cagctgtcca atggatatct gctccaggct ttatttgatt
     4201 gtatctttgt gaaacgtctc cgtcaaactg agaagctcct ccatcgatat aaacagacca
     4261 atgagtgtag tctgcagctg ataacgaagt tatcgttaac acagaaacta tcaaaaatgt
     4321 aaataacttt ttcatataac gtgttttagt taattattca ggggacaaat ataaaaacaa
     4381 tatttttatt tttttctttt ttttctataa tattttcatt tttgcctaat ttttagacta
     4441 ttactcgcaa atttcaccta aaagtgttgc taccgtggca tctttaattc gtatttcggc
     4501 gtattcgccg atttttttgt tttctttatt aaatacgacg actttatttt ccgaggattt
     4561 tccaaaaagc tgattgcgtg atttttttga atatccctcg attaaaactt caacaatttt
     4621 tccaagatct ttttggttac tttctttgga aagtttgttt tgtaaatcaa caacctcttg
     4681 cagtctcgat aatttgattg cctctggaac attatcttcg aggtgtttag ctgcgtatgt
     4741 gccacttcgt tcggagtatt taaacataaa ggcgttgtca aatccaactt cttgcataag
     4801 tgaaagcgtt tcttgatggt cggcttctgt ttcagaatgg aatcctgtaa aaacatcagt
     4861 ggttatacca cattctggga taatgcgttt tatcatagca attttttgca aatattcttc
     4921 acgagtatat tttctattca ttaaggacaa tatacgatta ctacctgatt gtacaggtag
     4981 atgaatgtgt ttgcataaat tatggtatgt ggcaatagtg cgaattaatt cttcgctaat
     5041 atcttttggg tgagaagtgg tgaagcggat tcttgtatca gggaatgtgt ttgcagccat
     5101 tgcaagtaaa tctggaaacg tgtacttgtt gtcttcagat gaatcaaggt atgaatttac
     5161 attttgacct aagaatgtta tttctttgta acctttggct actaaatctt tcatttcgtt
     5221 aataatactt gtagcagggc gacttctttc tctgcctcgt gtgtacggaa caatgcaata
     5281 agaacaaaaa ttattacatc ctcgcatgat ggacacaaac ccactaattc gattatgtcc
     5341 aatacgctgt ggtataatat cttgatatgt ttcggttgtc gaaagttcaa tatttatagc
     5401 tttctctcca gattctaccg ctccgatcag attaggcaaa tcaagatacg catctggacc
     5461 aacaaccatg tctatgactt ttcgttcaag taaatcttct ttaattcttt cagccataca
     5521 gcctaacact ccaataatga tatcttttct tttctttttt aacgattgaa agtaatcaag
     5581 gcgtgaaaaa gctttttgtt ctgcattgtc tctgatggaa caggtgttaa tgaagatggc
     5641 atttgcttcg tttatattgt ctgtgagctc gtatccactc atgcccataa ttgatgctac
     5701 aacttcgctg tctgcaacgt tcatttgaga tccgtatgtt tctataaata attttttttg
     5761 gtttttcatt ttatgtccga aat
//
LOCUS       DAOSMY010000056         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14087_length_5764_cov_4.069890, whole genome shotgun sequence.
ACCESSION   DAOSMY010000056 DAOSMY010000000
VERSION     DAOSMY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14087_length_5764_cov_4.069890"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1498)
                     /locus_tag="PLD76_02200"
     CDS             complement(149..1498)
                     /locus_tag="PLD76_02200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU26850.1"
                     /translation="MNTFDQLELTTPLRNAIADMGLIEPTQIQTEAFSVIRSGKDMVG
                     IAQTGTGKTLAYLLPILRDLGFSKQANPRILIMVPTRELVVQVVDTIKQITAYMNVRV
                     LGVYGGVNMATQQQAALQGMDIVVATPGRLYDLVMIRAIQLKSIAKLVIDEVDVMLDL
                     GFRPQLTNLFELMPQKRQNIMFSATMTEEVDALLDDFFTIPERITVAISGTPLDNIEQ
                     TCYPVKNFYTKANLLAYLLDNRNVFTKVLVFVSTKVAADRLFKELEEEFMDEIGVIHA
                     NKSQNYRLRSLEQFDNGEFRIMIASDVMARGLDLDKLSHVISFDTPKYPENYIHRIGR
                     TGRAKAKGKSILFYSPTEIVYKEAIENLMGITITEKSFPSKVQLSKRLTPDEQPKEEN
                     SIAQKRKPIEEKNPAFHEKKEKNKKVNLGGSYRRELAKKYKKPKTKGDKIANRKKKK"
     gene            complement(1669..3300)
                     /locus_tag="PLD76_02205"
     CDS             complement(1669..3300)
                     /locus_tag="PLD76_02205"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOU26851.1"
                     /translation="MTQKEQHKKLGDTLWDIANDLRGVMNADDFRDYMLSFLFLRYLS
                     FNYEEAAKKELGKDYPPSPSKDFRADFLVPPPLELWYKKNKGDINEFEEQMRKKVHYV
                     IKPKFLWSNITELARTQNGDLLETLQKGFRYIEDESFESSFKGLFSEINLNSEKLGKT
                     PKERNDKLCKIITKIAEGIADFSTDTDTLGDAYEYLIGKFAAGSGKKAGEFYTPQQIS
                     TILSKIVLLDSQDPTTGYKNKLNKVLDFACGSGSLLLNVRTQLKKAEGNIGKIYGQEK
                     NITTYNLARMNMLLHGMKDTEFEIFHGDTLLNQWDLLNEMNPSKKIEFDAIVANPPFS
                     LRWEPTDTLAEDFRFKGYGLAPKSAADFAFLLHGFHFLSKEGTMAIILPHGVLFRSGA
                     EARIREKLLKENYIDTVIGLPSNLFYSTGIPVCILVLKRCKKQDDVLFINASEQFVKG
                     KRQNELSEKHLDNIVETYKFRNERERYSRRVSLDEIKKNDYNLNISRYVSTSEDEIQI
                     DLEDVNIKLTEINKSIKVNTEKHNEYLKELGLKPI"
     gene            complement(3413..4861)
                     /locus_tag="PLD76_02210"
     CDS             complement(3413..4861)
                     /locus_tag="PLD76_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU26852.1"
                     /translation="MENWINTAIQLLQRSLKPIPQELNEIDWKSGLSDKSERLAQHIS
                     AFANHPLGGYLAFGIGNDGSSKPLSKEAMDEVVQKLGNIARNNLAQPIGIEHAVANFN
                     GEPVLFIKIPENQDKPVHLRGGDIYNSYKRSAGQTVKLSRQEVKSLIALSTGVDFESQ
                     TAQGNVHDDEVLKLVDYDSYFTLQEKRLPDTKLSILQTLANEDLIRKSVQGWDVLNLG
                     AVLFAKDLKAFKDLKRKAVRVIVYQETSRINALKEQEGAKGYASGFEGLINYIMDQLP
                     SNEVIENALRKQVKVYPEVAIREFVANALIHQDFSVTGSGVMVEIFTDRIEITNPGIP
                     LVDVNRFIDTAPKSRNESLASLMRRLNICEERGSGIDRAINAIEVFQLPAPKFIRGDD
                     YTRVILYAPTALTRMSIEDRIRACYQHTCLNYVNNQVVNNQSVRKRFKIAVNNVSMAS
                     KIISETIDTGLIKASDPENSSKKFASYVPFWA"
     gene            complement(4866..>5764)
                     /locus_tag="PLD76_02215"
     CDS             complement(4866..>5764)
                     /locus_tag="PLD76_02215"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOU26853.1"
                     /translation="AAGFNFFIWKSDDAYINQRTMKLNSKKKENDSLKVILEPLVHSK
                     INEIGTGQNNNLSKDALKEIEFPFPNPKEQVKIASCLSSLDDLIAAHSQKLDLLKDHK
                     KGLMRNLFPQEGEKVPKLRFKEFEKDGEWVENQLQEHCHNISSGKDKNQDDGKIDLYG
                     STGIIGKTENESYEGDYILVARVGANAGLLNRAIGKFGVTDNTLIINLKEPEMVDFIF
                     YSLDKIGLNKLVFGSGQPLVTGKQLKELNIPIPKNPKEQQKIASCLSALDELITSQLE
                     KIDQLQQHKKGMMQGLFPKIED"
BASE COUNT         1604 a         1114 c          962 g         2084 t
ORIGIN      
        1 tgcaagtagg cttgtatggg aaattctccc aattaatttg attgatgttg tacggggtag
       61 ttttttctac taacaaaagt gtatttgcaa gattgttact tggctctaca tatacaacta
      121 tatcctccat agatatcaat acaattattt attttttctt ttttctgttg gcaattttat
      181 ctccctttgt tttgggcttt ttgtattttt ttgccaactc tctgcggtac gaacctccta
      241 aatttacttt tttgttcttt tcttttttct cgtgaaaggc aggatttttt tcttctattg
      301 gttttcgttt ttgagcaatg ctattttctt ctttaggttg ctcgtcggga gtaagtcttt
      361 ttgaaagttg aacttttgaa ggaaaggatt tctccgtaat tgtgattccc atcaggtttt
      421 cgatagcctc tttgtaaaca atttctgttg gactataaaa taatattgac tttcctttgg
      481 cttttgctcg tcctgttcgt cctatgcgat ggatataatt ttcgggatat ttaggtgtgt
      541 caaagctgat aacatgactc agtttatcta aatccaagcc acgagccatt acatcagatg
      601 ctatcataat acgaaattcg ccattgtcaa attgctctaa tgaacgtaaa cggtagtttt
      661 ggcttttgtt ggcatgtatt acaccaattt cgtccataaa ttcttcttct aattctttga
      721 aaagcctatc ggcagcgact tttgtactga caaataccaa cactttcgta aacacatttc
      781 tattgtcgag taggtaggct agtaaattgg ctttggtata aaagtttttt acaggatagc
      841 acgtttgttc gatgttatca aggggcgtac cactaatagc aacagtaatg cgttcgggta
      901 tggtaaaaaa atcatctaac aaagcatcta cttcttccgt catagtagcc gaaaacatga
      961 tgttttgtcg tttttgaggc attagttcaa acaaattagt gagttgcgga cggaaaccta
     1021 aatctaacat tacatctact tcgtctatca ctaatttagc tatcgacttg agttgtatgg
     1081 cacgtatcat cactaaatcg tacagtcgac caggcgttgc tacaacaata tccattcctt
     1141 gaagtgctgc ttgttgttgt gttgccatat ttactccacc ataaacaccc aatacacgta
     1201 cattcatata ggcggtaatt tgtttaatgg tatctaccac ttgcacaaca agttcgcgtg
     1261 tgggtaccat tatcaaaatg cggggattag cttgtttaga gaaccctaaa tctcgcaaaa
     1321 ttggcaatag ataagccaat gttttacctg ttcctgtttg tgcaatacct accatatctt
     1381 tgcccgagcg aattaccgaa aaagcttcgg tttgtatctg tgtaggttca attaacccca
     1441 tatcagcaat agcgttacgc aatggggtgg tcagttcgag ttggtcaaag gtattcatag
     1501 gtataaaaaa tgcgttatgc aaattggaaa tcggtgcaaa ggtacaaaaa aaatgctagc
     1561 tatgtttcct gattttgata tcactaattt gctttatgat taatcagtga agattgccta
     1621 ataaaaacaa taatacatat gtatattgat atgtatgatt taataatctt aaatcggttt
     1681 caatcctaac tctttcaaat attcgttgtg tttttctgtg tttactttta tgctcttgtt
     1741 tatttcagtc aatttgatgt ttacatcttc caagtcaatt tgtatttcat cttctgaagt
     1801 acttacgtat cttgaaatgt tcagattata gtcgttcttc ttgatttcgt ccagtgaaac
     1861 tctacgagaa taacgctctc tttcatttcg gaatttataa gtctcaacga tgttgtccag
     1921 gtgcttctcg gaaagctcat tttggcgttt tccttttaca aattgctcac tagcattaat
     1981 aaataaaaca tcatcttgtt ttttgcatct tttcaatacc aaaatgcaaa caggaattcc
     2041 tgtcgagtaa aataaatttg atggtaaacc aattactgtg tcaatgtaat tttctttcaa
     2101 tagtttttct ctgattcttg cttctgcacc actacggaat aaaactccgt gaggcagaat
     2161 aatagccatt gttccttctt tagataaaaa gtgaaatcca tgtaagagaa aagcaaagtc
     2221 cgctgctgat ttaggagcta aaccatatcc tttaaagcga aaatcttctg ctaaagtgtc
     2281 ggttggctcc caacgtaaac taaatggagg attagctaca atagcatcaa actcaatttt
     2341 tttactcgga ttcatttcat tgagcaagtc ccattgattg agcaaagtat caccgtggaa
     2401 tatctcaaac tcggtatctt tcataccgtg taaaagcata ttcattcggg caaggttgta
     2461 ggtggtgatg tttttctctt gtccataaat tttgccaatg tttccttctg ctttctttag
     2521 ttgtgttctt acgtttaaga gcaaagaacc cgaaccacaa gcaaaatcca aaaccttgtt
     2581 caatttgttc ttataaccag ttgttgggtc ttgagaatct aacagaacaa ttttcgaaag
     2641 aatcgttgaa atttgttggg gcgtataaaa ttcacctgct ttttttcctg aacctgctgc
     2701 aaattttcca atcaaatatt cgtaagcatc tccaagcgtg tcagtatctg tagaaaaatc
     2761 tgctatgcct tccgctattt tggttattat cttacaaagt ttatcgtttc tttctttagg
     2821 tgttttaccg agtttttcag aattcaaatt gatttcagaa aacaaacctt taaaagaact
     2881 ttcaaacgat tcatcttcta tgtatcgaaa tcctttttgg agtgtttcta ataaatctcc
     2941 gttttgcgtt cgtgctaatt ctgtgatgtt gctccacaaa aattttggtt tgataacata
     3001 atgcactttt tttcgcatct gctcctcaaa ctcattaata tctcctttat tttttttgta
     3061 ccaaagttcc aaaggtggcg gcactaaaaa gtctgctcta aaatcttttg atggacttgg
     3121 cggataatct ttacctaatt ctttttttgc ggcttcttca tagttgaaag acaagtaccg
     3181 aaggaagaga aatgaaagca tataatcacg gaaatcatct gcattcatta cacctctcaa
     3241 atcattggca atatcccaaa gcgtatcgcc taatttttta tgttgttctt tctgtgtcat
     3301 tttttctctt ttaaacttta atccgtgttt tctaaacaaa taaataaatt attattaatc
     3361 agatctttac atattgttta ttggctgtcg tgattttctg atgaaataaa aattaagccc
     3421 aaaaaggtac ataggatgca aactttttag atgaattttc agggtctgaa gcttttatca
     3481 aacctgtgtc tattgtttcg gaaataattt ttgatgccat tgatacatta ttaactgcta
     3541 ttttaaatcg ctttcttact gattgattat ttacaacttg gttatttaca taattcaaac
     3601 aagtgtgctg ataacaggct ctaattctat cttcaatact cattctcgtt aatgcagttg
     3661 gtgcatataa aattactctt gtataatcat cacctctgat aaatttggga gcaggtagtt
     3721 gaaaaacttc aatcgcatta atggctctgt caataccact acctcgttct tcacaaatat
     3781 ttaatcgtct cattaaagaa gcaagacttt catttcttga tttgggtgca gtatctatga
     3841 atcggtttac atccactaat ggaatacccg gattcgtaat ttcaatccta tctgtaaata
     3901 tttcaaccat cactcctgag ccggtaactg aaaaatcttg atgaatcaat gcattggcaa
     3961 caaactcacg gatagcaact tctggatata cttttacttg ttttctcagt gcattttcta
     4021 ttacctcatt agaaggtagt tggtccatta tatagttgat gagtccctca aaacccgatg
     4081 cgtacccttt tgctccttct tgttctttta aggcgtttat ccggcttgtt tcctgataaa
     4141 ctatcacacg gaccgccttc cgtttcaggt ctttaaaagc ctttaaatcc ttagcaaata
     4201 aaacagctcc taaatttaaa acatcccatc cctgaactga ttttctaatt aaatcttcgt
     4261 ttgccaacgt ttgcagaatg ctcaacttgg tgtctggcaa tcgcttttcc tgaagtgtga
     4321 aatagctgtc ataatctacc agctttaaaa cttcatcatc gtggacattg ccttgtgcgg
     4381 tttgagattc aaaatctaca ccagttgaca acgcgattaa tgattttact tcttgtcttg
     4441 agagttttac tgtctgtccg gcactacgct tataactatt gtagatatcc cctcctctta
     4501 aatgaacagg tttgtcttgg ttttcaggga ttttgataaa tagaacaggc tctccgttaa
     4561 aatttgctac tgcgtgttct ataccaatgg gctgtgctaa attgtttcgg gctatattgc
     4621 ccaatttttg aacaacttca tccatcgctt ctttactaag tggttttgag gaaccatcgt
     4681 ttccgatacc aaaagccaga tatccaccca atggatggtt agcaaaagca gaaatatgtt
     4741 gcgctaggcg ttcgcttttg tctgacaagc cactcttcca gtctatctca ttcaactcct
     4801 gtggaatagg tttaaggctt ctttgtaaaa gctgtatggc tgtatttatc caattttcca
     4861 taattttaat cttctatttt aggaaacaat ccctgcatca tacccttttt gtgttgctgc
     4921 aattggtcaa ttttctccag ctgagaagtg atgagctcat ccaaagccga aagacaggaa
     4981 gcaatttttt gttgttcttt ggggttcttg ggaatcggaa tatttaattc tttaagttgc
     5041 ttgcctgtca ctaggggctg accagagccg aaaaccagct tgttcaaccc aattttatct
     5101 agtgaataga agataaaatc caccatttca ggttccttga gatttatgat caaagtatta
     5161 tcagtaactc caaatttacc gattgccctg tttaataaac ctgcattagc accaacccta
     5221 gcaacaagaa tgtaatcgcc ttcgtaactt tcgttttcag tttttccaat tatcccagtt
     5281 gaaccatata aatcgatttt accatcatcc tgatttttgt cctttccaga agagatgttg
     5341 tgacaatgtt cttgtagctg attctccacc cactccccat ctttctcaaa ctccttaaac
     5401 ctcaacttcg gcactttttc accctcttgt ggaaaaagat ttcgcatcaa gccttttttg
     5461 tggtctttga gtaaatccaa cttttggctg tgggctgcaa tcaaatcgtc taaagaagat
     5521 agacaggaag cgattttgac ttgttctttt ggatttggaa acgggaactc aatttcttta
     5581 agggcatctt tagaaagatt gttattttgc cctgtcccaa tctcattaat ttttgaatga
     5641 accaatggtt caagtataac ctttaggcta tcgttttcct ttttcttgga attcagtttc
     5701 attgtacgct gattaatata agcgtcatct gatttccaaa tgaaaaaatt aaaacctgct
     5761 gctc
//
LOCUS       DAOSMY010000057         5761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14098_length_5761_cov_4.179635, whole genome shotgun sequence.
ACCESSION   DAOSMY010000057 DAOSMY010000000
VERSION     DAOSMY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14098_length_5761_cov_4.179635"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLD76_02220"
     CDS             complement(<1..545)
                     /locus_tag="PLD76_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU26854.1"
                     /translation="MNYYSTNKNASIATLQKAVVKGLAEDKGLYMPETIKLLPPQFYD
                     NIDSMSFQEIAYEVAQAFFGEDIEEGALKQIVYDTLNFDVPLVPVTETIYSLELYHGP
                     TLAFKDVGARFMSRLLGYFIEKEGEKNVKVLVATSGDTGSAVANGFLGVKGIHVYVLY
                     PKGLVSPIQECQFTTLGQNITA"
     gene            complement(561..1769)
                     /locus_tag="PLD76_02225"
     CDS             complement(561..1769)
                     /locus_tag="PLD76_02225"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOU26855.1"
                     /translation="MKYIIILGDGMADEPIAKLGNKTPLQFANTPYIDKVAELGCSGM
                     LHTIPDGFHPGSEIANLSVLGYKVADVFEGRGSLEAASMGVDIENNEMAMRCNLICIE
                     NGIIKNHSAGHISNEEAAILIADLEKELGNDVVSFYAGVSYRHLLKIKGGNKNITCTP
                     PHDVPGTAFSTVMIEANVPEAQPTADLLNDLILKSQAILENHPINLKRAAQGKDKANS
                     IWMWSPGYKPKMKTLLELYNLKSGSVISAVDLIRGIGVYAGLKVILVEGATGLYNTNY
                     EGKAQAAIEALRTDDFVYLHIEASDEAGHEGDVDLKVRTIEYLDQRIVKTILEEVSTW
                     SEPVCIAILPDHPTPCALKTHTTDPIPFVIYKPDGKKDDVTVYDEFAARKGGYGEIRE
                     QEFMQLLLQQ"
     gene            complement(1782..2831)
                     /locus_tag="PLD76_02230"
     CDS             complement(1782..2831)
                     /locus_tag="PLD76_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOU26856.1"
                     /translation="MISKIILEDLISQTKKAIDKSTSIVIVSHVNPDGDAIGSSLGLY
                     HFLFSQGKETRIIMPNSFPNFLNFLPDSSDILIYDDYQDEAIDLIESADLVFCLDFNQ
                     LKRVGAMEDVIKNCKATKILVDHHPFPDTFANITISHPEIASTSELIFRMICRMGYFT
                     EMNIHSAECICTGIMTDTGAFSYNSNSPEIYTIISELIKKGVDKDALHRKLYNNYSAQ
                     RMQLMGYLLSQKMKIYAEYGTAIMVLSLAEQETYHHKKGDSEGFVNLPLSIHGITFSI
                     FLKEETDTIKMSFRSQGNFPSNTFANRYFNGGGHLNASGGEGLGTIEETVTYIESILP
                     AFYQEWVDNNNNKEI"
     gene            complement(2876..4936)
                     /locus_tag="PLD76_02235"
     CDS             complement(2876..4936)
                     /locus_tag="PLD76_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOU26857.1"
                     /translation="MSSTPLFHQIPFFRLTSALVIGIFISSVVPLSLTIGYILLFLSI
                     IGVIISNVIDKRKTLSYSFRWIYGVSIIGIFMSLGMLLYQHKIQELSLELPDNKKTFI
                     ATISESPIEKPKSVMTVVTIAVPEINKTHRAILYLEKDSASKTVQYGDYLLVNTLLQK
                     PRNTGNPYEFDYSTFLLHKGITATSYINKGSWKQIKKTSSFSVTKHAINTRNKLLNIY
                     KKYGITDNEFGIVAALTVGYKEGLSKETKQSFSITGGSHVLAVSGLHVGIIYLALGFM
                     LGFMAKSKQGIVGKQIILIASIWAYAFICGLPPSVVRASIMISLICISEILNRKSATY
                     NAVFVSGFCMLIYNPLYLFDVGFQLSYMAVLSILYFQPRINNIFSPNHIVIKWLWSLT
                     AVSIAAQIGTAPISLYYFHNFPTYFLLTNIIVIPAATIIIYLSIVLLLLSSWVKIGLI
                     VAWLLKSCVQIISIGIHFIEHLPGASIPDIWIPIWFMPIVFLSLVSTILFIRTQRSRY
                     FFLIFYLSILSISIAIWNRFQTLQNKELIVYANNPSETNFNIHIISGESNTLLADTIY
                     KANLEQYVNTVTHKKHLNPARFEHIDMKNSFGCIDTLTYFVYTNPTKWKPIDISIPVD
                     IVFVYEKRMCHVTEITSHVSPTYIVFGENINGKQLQILKEECEKKSINYHSLYEKGAF
                     ILPL"
     gene            complement(4946..5596)
                     /gene="rpe"
                     /locus_tag="PLD76_02240"
     CDS             complement(4946..5596)
                     /gene="rpe"
                     /locus_tag="PLD76_02240"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU26858.1"
                     /translation="MATLIAPSLLAANFAHLQKDIEMINQSTADWLHLDIMDGVFVPN
                     ISFGFPVIESIKDICQKPLDVHLMIVEPDKFIADFATVGTYMLNVHYEACTHLHRTIQ
                     KIKAANMKAAVTLNPHTPICVLQDIIQDVDMVLLMSVNPGYGGQKFITNTLQKTADLK
                     ELILKKNAKALIQIDGGVNLTTGRQLVEQGADVLVAGSFVFASTNPLQTIAELKKL"
BASE COUNT         1816 a         1026 c          967 g         1952 t
ORIGIN      
        1 gctgtaatat tttgtcctaa tgtagtaaac tgacattctt gaatagggct aaccaatcct
       61 ttaggataca gcacatatac atgaatacct ttaacaccga gaaatccatt agctacagca
      121 ctgcctgtgt cgccagaagt ggcaaccaat acttttacat tcttttctcc ctctttttca
      181 ataaaatagc caagcaaacg cgacataaaa cgtgcaccta catctttaaa agccaaggtg
      241 ggtccgtggt ataattctaa actgtaaata gtttcggtaa caggaaccag aggtacgtca
      301 aagtttaatg tgtcgtacac aatttgcttt aaagctccct cctcaatgtc ttctccaaaa
      361 aaagcttgag ccacttcgta agcaatttct tgaaaagaca tactatctat gttgtcataa
      421 aattgcggag gtaataactt aatagtttct ggcatataca agcctttgtc ttcagctaag
      481 ccttttacaa ctgctttttg taaggtagct atagatgcat ttttattggt gctgtaataa
      541 ttcatattga ctatataggt ttattgttgt aataataatt gcataaattc ttgttctcga
      601 atttctccgt agcccccttt gcgtgcagca aattcatcgt acaccgttac atcatctttt
      661 tttccatcag gtttataaat aacaaaaggg atggggtctg ttgtatgtgt ttttaaagca
      721 catggggtag ggtgatcggg taaaatagca atgcatacag gttcgctcca agtgcttact
      781 tcttctaata ttgtttttac aattcgttgg tcgaggtatt caatggttct tacttttaag
      841 tcaacatccc cttcgtgacc agcttcatcg cttgcttcga tgtgtaggta cacaaaatca
      901 tcagtgcgta atgcttcaat tgctgcttgt gcttttcctt cgtaatttgt gttgtataag
      961 ccagtagccc cttctactaa aataactttt aagccagcgt acacgccaat tccacggatt
     1021 aaatctaccg ctgatattac tgagccactt tttaaattat acagttcaag cagggttttc
     1081 atttttggtt tgtatcctgg cgaccacatc caaatactat ttgctttgtc tttcccttga
     1141 gccgctcgtt ttaggttaat tgggtggttc tccaaaattg cttgcgattt taaaattaga
     1201 tcgtttaata agtcggctgt tggttgtgcc tcgggcacat ttgcctcaat cataacagta
     1261 gaaaaagctg tgcccggtac atcatgggga ggagtacaag tgatattttt atttcctcct
     1321 ttaattttta gtaaatgacg atacgataca cctgcataaa aactcaccac atcattgccc
     1381 aattcttttt ctaaatcagc aatcaaaatc gccgcttctt cgttgcttat atgtccagca
     1441 gagtggttct tgataatgcc attttctata caaataaggt tgcaacgcat tgccatttca
     1501 ttattttcaa tgtcaacgcc catactagcc gcttctaacg aacctcttcc ttcaaacaca
     1561 tcagctacct tgtatccgag tacgcttaaa ttggcaattt cgctacctgg atgaaagcca
     1621 tcaggtatgg tatgtagcat gccactacaa ccaagttcag ctactttgtc gatatacgga
     1681 gtatttgcaa attgcaaggg tgttttgttt cctaatttag ctattggctc atcagccatt
     1741 ccgtctccaa gtatgataat atatttcatc taagtataag tttatatttc tttgttattg
     1801 ttattatcaa cccattcctg ataaaatgca ggtaatatgg attcaatata cgtaactgtt
     1861 tcttcgattg tacctaaacc ttcaccccca gaggcattta aatgaccacc accattaaaa
     1921 taacggttgg caaacgtatt gcttggaaaa ttaccttgcg agcggaacga catttttatt
     1981 gtgtctgttt cttctttcag aaaaatagaa aatgtgatgc catgtatgga taatggcaaa
     2041 ttaacaaaac cctcgctatc tccttttttg tggtgatagg tttcttgttc tgccaaagac
     2101 aaaaccatga ttgcagttcc gtattcagca tagattttca ttttttgcga caataaatat
     2161 cccattaatt gcatccgttg tgctgaataa ttattatata attttctgtg taatgcatct
     2221 ttatcaacac ccttttttat caattctgaa ataatggtat agatttcggg actattggaa
     2281 ttgtacgaaa aagctcctgt atcggtcata atacccgtac aaatgcattc tgctgaatga
     2341 atgttcattt cagtaaagta tcccatccta caaatcatac gaaaaattaa ctcacttgtg
     2401 gaggcaattt cgggatgtga tatagtaatg ttagcaaagg tatctggaaa tgggtgatga
     2461 tctactaata tttttgttgc tttgcaattt tttattacat cttccattgc acctacacgt
     2521 tttagttgat taaaatccag acaaaaaact aaatcggcag attcaattaa gtcgatagct
     2581 tcatcttgat aatcgtcata tatcaagata tcactactgt cgggtaaaaa gttaaggaaa
     2641 ttagggaaag aatttggcat aataatacga gtttcttttc cctgagaaaa caaaaaatga
     2701 tacagcccta aagaactacc aatggcatct ccatcagggt tgacatgaga tacaatcaca
     2761 atactagtgg atttatcaat tgcttttttt gtttgtgaaa ttaaatcttc gagaataatt
     2821 tttgatatca tattatcact attagtaaat ctactgcaaa aatagtaata ctttgttata
     2881 gtggaagtat aaatgctcct ttttcgtaca acgaatgata gttaatgctc tttttttcac
     2941 attcttcttt caatatttgt agctgttttc catttatatt ttctccaaat acaatgtagg
     3001 tgggagatac atgggacgta atttctgtta catgacacat tcttttttca tatacaaaaa
     3061 caatatctac tggtatagat atgtcaatgg gtttccattt tgtggggttg gtatacacaa
     3121 agtaagtgag tgtatcaata caaccaaaag aattcttcat atcgatgtgt tcgaaacgtg
     3181 ctggattcaa gtgtttctta tgggttactg tattcacata ctgttcgagg tttgccttat
     3241 atatagtatc tgctaacagt gtatttgatt ctccagaaat aatatgtata ttaaaattcg
     3301 tttctgaagg gttgtttgca tagacaatta gttctttatt ttgcaatgtt tgaaatctat
     3361 tccagattgc aatcgaaatg gataaaatag ataaatagaa gattaaaaag aaatatcttg
     3421 atcgctgagt gcgaataaaa agtatggtac taacaagtga taaaaaaacg ataggcataa
     3481 accaaatcgg aatccaaatg tcaggtatgc ttgctcctgg taaatgctca ataaaatgta
     3541 tcccaattga aataatttgc acacaacttt ttagtaacca tgcaactatt aatccaatct
     3601 ttacccaaga agaaagcaat aaaagtacta tagaaagata aataataatt gtagcagctg
     3661 gaatgactat aatgtttgta agtaaaaaat atgttggaaa gttatgaaaa taatataaac
     3721 taataggagc tgttccaatt tgtgctgcaa tagaaactgc cgtcagactc cataaccatt
     3781 tgattactat atgattgggg gaaaatatgt tattgatacg aggctgaaaa tacaagatac
     3841 ttaatacagc catatagctt aattgaaaac ctacgtcaaa taagtataac gggttgtaaa
     3901 tcagcataca aaacccacta acaaatacag cattgtatgt agccgatttt ctgttcaata
     3961 tttcgctgat acaaattaag gatatcatga ttgatgctct tacaactgat ggaggaagac
     4021 cgcaaataaa agcatacgcc caaatacttg ctatgagtat aatttgtttg cctactatcc
     4081 cttgtttgct ttttgccata aatccaagca taaacccaag tgcaagataa ataataccaa
     4141 catgtaagcc acttacagcc aatacatgag atcctccagt aatcgaaaaa ctttgttttg
     4201 tctctttaga taatccttcc ttatatccca ccgtcagtgc tgcaacaata ccaaattcat
     4261 tgtctgttat accatatttt ttatagatat tgagcaattt attgcgggta ttgatagcat
     4321 gcttcgtaac tgaaaaagag gatgtttttt ttatttgttt ccaacttcct ttattgatgt
     4381 atgatgtggc agtaatgcct ttgtgtaata aaaatgttga ataatcaaat tcgtatgggt
     4441 ttcctgtatt tcgcggtttt tggagtagtg tgttcactag cagataatca ccatattgta
     4501 ctgttttcga tgcactgtct ttttctagat atagtatagc tctatgtgtt ttatttattt
     4561 ctggaactgc tattgtaaca accgtcatta ctgattttgg cttttcgata ggagattcac
     4621 taattgtggc aataaatgtc tttttgttgt caggaagttc aagacttaat tcttgtattt
     4681 tgtgctggta taagagcatt ccaaggctca taaatattcc tataatggat acaccatata
     4741 tccaccgaaa agaatagctc aacgtttttc ttttgtctat tacgttggaa ataatcactc
     4801 cgatgattga caaaaaaagc aatatgtaac caatagtgag gcttaaggga actacagaac
     4861 ttataaatat gccgataacg agagctgatg taagtctaaa gaaagggatt tgatgaaaca
     4921 aaggagtaga tgacattacc tagcatcaaa gtttttttaa ttcagcaata gtttgtaaag
     4981 gattagtgga ggcaaaaaca aaacttccag caactaacac atccgcacct tgttcaacga
     5041 gttgcctacc cgtagtaaga tttactcctc catcaatttg aatcaaagct ttagcgtttt
     5101 ttttcagtat caactctttt aaatcagccg ttttctgtaa ggtattagta ataaattttt
     5161 gcccaccata tcctggattt actgacatca atagtaccat atctacatct tgaataatat
     5221 cttgtaaaac acaaatagga gtatgaggat taagtgttac agctgctttc atgttggctg
     5281 ctttaatttt ttgtatcgtg cgatgaagat gtgtacatgc ttcataatga acatttagca
     5341 tatatgtgcc cacagtagca aaatcggcaa taaatttatc tggctcaaca atcattaaat
     5401 gcacatctaa tggtttttgg cagatgtctt taatgctttc tataacagga aagccaaaag
     5461 aaatattggg aacaaatacc ccatccataa tatccaagtg taaccaatca gcagtacttt
     5521 ggttaatcat ttcaatgtct ttttgcaaat gagcaaaatt ggcagctaat aatgatggag
     5581 caataagtgt agccataatg taaattttta cactacaaaa ttaaataaaa taaatgataa
     5641 gctcactgat ttttccaatc agtttagaat aatttcaaaa aaactgccat tatctatttt
     5701 ttgaacatgg taacatgctg caaacataag aattttgttt accgtttctg ccttgggttg
     5761 c
//
LOCUS       DAOSMY010000058         5758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14107_length_5758_cov_3.654568, whole genome shotgun sequence.
ACCESSION   DAOSMY010000058 DAOSMY010000000
VERSION     DAOSMY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14107_length_5758_cov_3.654568"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PLD76_02245"
     CDS             <1..1606
                     /locus_tag="PLD76_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOU26859.1"
                     /translation="NCIVKDMSRIGRDHLRVGLLLEQFRESGVRFIAVNDNVDTDKGM
                     DDFTPFRNIINEWVARDTSRKIRTIFNARTAQGKHVTGALPYGYLHDPNDRQNWLLDE
                     VAAPIVKRIYKSIIEGKTIAKIAEELTKEKVLCPNAHWQNINEATSKGRPSADPYKWS
                     SATIVTITAKQEYMGWKVLNKTTKENYKAKRKPTPKEDLIIFKDMHPVIIDEEMWNVV
                     QRLRETKRSTSKLDGKPNPLTGVLYCADCGQKMYHKKGSTGRENQPHHEYVCSSYRHY
                     SRTCTCHYIRVPVVEDLILRTIQQVSKYVNENEEEFIAKVHENAVIIGEETIKANRKK
                     ITTSKRRREEISGLVKKLYETYATGKISEEHFTELINAYSNEQQNIDAETELLNAEIK
                     KFNTESVKADKFIDLVKRHTELAEFTPLLLNEFIEKVVVYEPVKENGKRNQRVDIYLN
                     FIGQFTHPITEELPQEQPKKGSRGRKLRHEMTEEEIQKERERDRERYARKVADKKAKE
                     QAERLAILQGTKYEIKISEEQINKGA"
     gene            1612..2001
                     /locus_tag="PLD76_02250"
     CDS             1612..2001
                     /locus_tag="PLD76_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HOU26860.1"
                     /translation="MAQSIFEKNGGKYIKVGDYYVPDLGQFDEDTEPVDERVIGKYGS
                     LRRTFLEEHRPAIYTKLLYSFELDTHLKDVNEEAQNLIDKLLPQYKKQQGVTEELKAQ
                     NQLLWVGKVNNIIAQIEEIIYREIVYV"
     gene            2091..2399
                     /locus_tag="PLD76_02255"
     CDS             2091..2399
                     /locus_tag="PLD76_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26861.1"
                     /translation="MAKKTLVEQIKDTQIELEKVRAEKKQKENQLAKIQKQEREKEHR
                     VRTRRLIQKGAVLESVDPNLAQLNEEQLKACLREIFSNHTVQEVAKNHLLSQQEVDTP
                     "
     gene            2568..4022
                     /gene="mobQ"
                     /locus_tag="PLD76_02260"
     CDS             2568..4022
                     /gene="mobQ"
                     /locus_tag="PLD76_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HOU26862.1"
                     /translation="MAIFHCDIKVISRGKSKSAVASSAYRSGTKMTNEYDGEIHDYTR
                     KGGIHSSAILLPSHAPKEYADRSTLWNAVEMIEKQHNSQLAREIEVSIPKEIPSHLWQ
                     RMMIDFCNTNFVKQGMIADLSVHNKDPNNPHCHIMLTMRPIKENGKWGAKSRKEYVLD
                     DNGNRIKLASGNWKSTKVDTMDWNSQDNAELWRTNWSEHCNLYLENLGHTERIDHRSY
                     ERQGIDKIPQIHLGVSASQMEQKGIKTDRGNRNREILNDNKQMKILRARITRLMKWQR
                     ELEAQPLDIAKAEIKTSVLGKLYANKDVVKNQNKTVRNLKADNKVHNFMQTHNIETMA
                     DFSAKVVDLNTSFYELKKLSKANNTAIADMDNRLKLWSEYEPLKKYIFKYNELNSKDK
                     KAFITKYRDKFNRYDELQKVWKEIEATEGKVTPKQWQKTHSTLIDDNKLCEWKMKALK
                     EEIGTAERIQKLLDEEVKEQNQAKDKKRVTENEL"
     gene            4268..4876
                     /locus_tag="PLD76_02265"
     CDS             4268..4876
                     /locus_tag="PLD76_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU26863.1"
                     /translation="MKVKDPTSETAIKLIQSATELFNDFGYTGTSINDIAKKSKLSKG
                     ILYHYFQSKDDVYLFCLSECINNFVDFMERNISKISLSKETMIRVVELRFVFFDENPQ
                     YKNLFHNIISGKPTHLSKEISEIRQVLTENNISWIKLILTEINLGKDVLESDVSLFIS
                     ILQNSSTFLLDAESDKKYRQDMINSIVRLTIIFLNGLREDLI"
     gene            4889..5500
                     /locus_tag="PLD76_02270"
     CDS             4889..5500
                     /locus_tag="PLD76_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU26864.1"
                     /translation="MKYIITIGREHGSGGREIGYKLAEEFGIPFYDSELVNRICKEKG
                     ISEEFFSEYDEKKGSLIYSLIMDLYAKEANVIENTSLEQKLFNIQRDVINDIADEGAC
                     VIVGRCADHILKDRENTISIFISADTDSKIQRVSTRNKLNAQDAKKKMKFTDNKRKNY
                     YNYYTSKKWGSSYCYDFCIKSNKLGIEQTVSTLKNMIDFLERG"
     gene            5502..>5758
                     /locus_tag="PLD76_02275"
     CDS             5502..>5758
                     /locus_tag="PLD76_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26865.1"
                     /translation="MEILIGILKIILAIVLATIVGKGVAKLKLPAILGWLITGMIIGP
                     YAFGLLDNELLNAQWYKNISHFFECAVGLMFAKELIFKKLKA"
BASE COUNT         2285 a          842 c         1121 g         1510 t
ORIGIN      
        1 aaactgcata gtcaaagata tgtcaaggat tggacgtgac cacttgcgtg taggacttct
       61 gttagagcag tttagagaga gtggggttcg ctttattgct gttaatgata atgtcgatac
      121 tgacaaggga atggacgatt ttacaccctt tagaaatatc ataaatgaat gggtagcaag
      181 agatacaagt cgaaaaatac gtaccatctt taacgcaaga acagcacaag gaaagcacgt
      241 tacaggtgca ttgccctatg gctatctaca cgaccctaac gaccgtcaaa attggctttt
      301 ggatgaagtg gctgcaccaa tagtcaaacg catttataag agcattatcg agggtaaaac
      361 aatagctaaa attgcagagg aattgaccaa agaaaaagtt ctttgtccta atgctcactg
      421 gcaaaatata aatgaagcta caagtaaggg tagaccgagt gcagacccat ataaatggtc
      481 gtctgctact attgtcacaa taacagccaa gcaagaatat atgggttgga aagtgctaaa
      541 caagaccaca aaagaaaatt ataaggcaaa acgaaagcct actccgaaag aagatctaat
      601 catcttcaaa gatatgcacc cagtcatcat tgatgaagaa atgtggaatg ttgtacaaag
      661 gcttagagaa accaaacgaa gtacaagtaa acttgatgga aaaccaaatc cgttaactgg
      721 agtattgtat tgtgctgatt gtgggcaaaa aatgtatcac aaaaaaggta gtacaggtag
      781 agaaaatcaa ccacatcatg aatatgtgtg ttcaagttat aggcactact cacgcacttg
      841 cacttgccac tatatccgag tgccagttgt agaagattta atacttagaa cgatacagca
      901 agttagcaaa tatgtaaatg aaaatgaaga agaatttatc gcaaaagtcc atgaaaatgc
      961 agtcatcata ggagaagaaa ccataaaggc aaatcgtaaa aagataacaa cttcaaaacg
     1021 tagacgagag gaaataagtg gcttagtaaa aaaactttat gaaacctatg caacaggaaa
     1081 aatctccgaa gaacacttta cggaactgat taatgcttat tcaaatgaac aacaaaatat
     1141 tgatgctgaa acagaactat taaatgctga aatcaaaaaa ttcaatacag aaagcgtgaa
     1201 agctgataag tttatagacc ttgtaaaaag gcatacagaa cttgcagagt ttactccact
     1261 tctactcaat gaatttattg aaaaagttgt tgtctatgag ccagtaaaag aaaatggaaa
     1321 acgaaatcaa agggttgata tttatcttaa tttcataggg cagtttacac atccaataac
     1381 agaagaacta ccacaagaac aacctaaaaa aggttcaaga ggtagaaaat tacgtcacga
     1441 aatgacagag gaagaaatac aaaaagaacg tgaacgtgac agagagcgtt atgcaagaaa
     1501 ggtggctgat aaaaaggcaa aagaacaagc tgagagatta gcaattttgc aaggcacaaa
     1561 atatgagatt aaaataagtg aagaacaaat caataaagga gcgtgagcta aatggcacaa
     1621 agcatatttg aaaaaaatgg tggcaagtac atcaaggttg gtgattacta tgtcccagac
     1681 ttagggcagt ttgatgaaga taccgagcca gtagatgaaa gagttatcgg caaatacgga
     1741 agccttcgcc gaacattttt agaagaacat agacctgcaa tttatacaaa actactttac
     1801 agttttgaac ttgatacaca cttaaaagat gtcaacgaag aagcacaaaa cctaattgat
     1861 aaacttctac cccagtataa aaaacagcaa ggtgttaccg aagaattaaa ggcacaaaat
     1921 cagctacttt gggtaggaaa agtaaataac attatcgcac aaattgaaga aatcatatac
     1981 agagaaattg tatatgtata acttaggatt taagacggag caagtaatat tgtttccgtc
     2041 ttttttcata taaaaaatca aatcaaaaac ttaaaaagga gcatttaatt atggcaaaga
     2101 aaacactggt agagcaaatt aaagatacgc aaatcgaact tgagaaagtg agagcagaaa
     2161 agaaacaaaa ggaaaatcaa ttagctaaaa tccaaaagca agaacgagaa aaagaacaca
     2221 gagtacgcac acgcagacta attcaaaaag gtgcagtttt ggaaagtgtt gatccgaacc
     2281 ttgcacagct taatgaagaa caattaaagg cttgtttaag agaaatcttt tcaaaccaca
     2341 cagtgcaaga ggtggcaaaa aatcatctac tatcacagca agaggtagac acgccataac
     2401 tcccttgtta gcaagggcgc acttatacac cattaaaatg gtgcgtgcgt tctgcgaagc
     2461 aatggggagc tactctcccc aaaccccttg tgtacttcac gaaactggct acaccttgcg
     2521 aggtgtagtc attttcataa agtttaataa aatcgaaagg agcagttatg gcaatatttc
     2581 attgtgatat taaagtaata agtcgaggga aaagcaagtc tgctgttgct tcatctgcat
     2641 atcgaagtgg cacgaaaatg acaaacgaat atgatggaga aattcacgac tacacacgca
     2701 aaggtggtat tcattccagt gcaattcttt tgccctctca cgcacctaaa gagtatgcag
     2761 accgtagcac tttatggaac gctgtcgaga tgatagaaaa gcaacataac tcacagcttg
     2821 caagagaaat tgaagtatca ataccgaaag aaataccctc acatctatgg caaagaatga
     2881 tgatagattt ttgcaatact aactttgtaa agcaaggtat gattgctgat ttatccgttc
     2941 ataacaaaga ccctaataac ccacattgtc acataatgtt gactatgcga cctattaaag
     3001 aaaatggtaa atggggagca aaatcacgca aggaatatgt acttgatgat aatggtaatc
     3061 gcattaaact tgcaagtgga aattggaagt caacaaaggt tgatacaatg gattggaaca
     3121 gccaagacaa cgcagaacta tggcgaacaa actggagtga gcattgcaat ctctatcttg
     3181 aaaatttagg gcatacagaa cgcatagatc accgaagtta tgaaaggcaa ggcattgata
     3241 aaattcctca aattcattta ggtgtttcag cttcacaaat ggagcagaaa ggcattaaga
     3301 cggatagagg aaatcgcaat cgagaaatct taaacgacaa taagcaaatg aagattttaa
     3361 gggcgagaat aacaaggctg atgaagtggc aacgagagct tgaagcacag ccacttgata
     3421 tagcgaaagc agagataaag acttctgttt taggtaaact atatgcaaat aaagatgtag
     3481 taaaaaatca aaataagact gttcgtaacc tcaaagcaga taacaaagtt cacaacttta
     3541 tgcagacaca taacattgaa acaatggctg attttagtgc aaaggttgtt gaccttaata
     3601 caagttttta cgaactcaaa aagctatcta aggcaaacaa tactgccatt gctgatatgg
     3661 ataataggct taaactttgg agcgaatacg agccattaaa gaaatatatt ttcaagtata
     3721 atgagttaaa ttctaaagat aaaaaagctt ttatcacaaa gtatcgtgac aagtttaatc
     3781 gttatgatga actgcaaaaa gtatggaaag aaattgaagc caccgagggt aaagttacac
     3841 ctaaacagtg gcaaaaaaca cactctacac tcattgatga taacaaactt tgcgaatgga
     3901 aaatgaaagc cttgaaagaa gaaatcggca cagcagagcg aatacaaaag ttgcttgatg
     3961 aagaagttaa agagcaaaac caagcaaaag ataaaaagcg tgtaacggaa aatgagttat
     4021 aaaaacatac aaatattggg taatttagca atagactttt tgtataaatg catgatataa
     4081 tagttaaatc aaaaccacat cattaagatg tgagattatt tatatacact ttagcaggct
     4141 tgaaatatag cctgctattt ttatattgca atgaatttaa aaaaatgtcg aaaactattg
     4201 accgattggt caatgtgtgt tattatatta ttgaccaatc ggtcaaaaat ataataagga
     4261 ggtttttatg aaagtaaaag atccgacatc agaaacagct ataaagctaa tccaaagtgc
     4321 aacagagtta tttaatgatt ttggatatac tggaacatct ataaatgata tagcaaaaaa
     4381 atctaaactt tcaaaaggta tactgtacca ttattttcaa agcaaagatg atgtgtattt
     4441 gttttgttta agcgaatgta taaataattt tgttgatttt atggagagaa atatatcaaa
     4501 gatatcactt agtaaagaaa caatgattag ggttgtagag ctaagatttg tttttttcga
     4561 tgaaaatcca caatataaga atttgtttca caacatcata tccggtaaac caacacacct
     4621 ttcaaaagaa atatcagaaa tacgacaggt tttaacagaa aataatatta gctggattaa
     4681 gttaattcta acagaaatta atttaggtaa agatgtatta gagagtgatg ttagtttgtt
     4741 tatttctata ctgcaaaata gttcaacatt tttgctggac gctgaaagtg ataagaaata
     4801 ccggcaagat atgataaatt cgatagttag actaacaatc atttttttaa atggattaag
     4861 agaggattta atttaaagga gatttttaat gaaatatata attacaatag gtagagaaca
     4921 tggaagtggt ggtagagaga ttggttataa attagccgaa gaatttggta taccatttta
     4981 tgatagcgag cttgtaaaca gaatttgtaa agaaaaagga atatctgaag agtttttttc
     5041 tgaatatgat gagaaaaaag gcagtttgat ttattcgctg attatggatt tatatgcaaa
     5101 agaagctaac gtaatcgaaa atacttcttt agagcaaaag ttattcaata ttcaaagaga
     5161 tgtaataaat gacattgcag atgagggtgc ttgcgtaatt gttggtagat gtgctgatca
     5221 cattttgaag gatagagaaa acacaataag tatttttatt tcagccgaca cagatagtaa
     5281 aattcagcgt gttagtacta ggaataaact taatgcacaa gatgctaaaa agaaaatgaa
     5341 attcactgat aataagcgta aaaattacta caactattat acaagcaaaa aatggggttc
     5401 ttcatattgt tatgattttt gtattaagag taacaaactt gggatagaac aaacagtttc
     5461 tacgttaaaa aatatgattg attttttgga aagaggataa aatggaaatt ttgattggaa
     5521 tattaaaaat tatattagct attgtgcttg caacaatagt tggaaaaggt gttgcaaaat
     5581 taaaactacc tgcaatttta ggatggttaa ttacaggtat gattatcgga ccttatgctt
     5641 ttgggctgtt agataatgaa ttgttaaatg cgcaatggta taaaaatatt agccactttt
     5701 ttgaatgtgc agtagggttg atgtttgcaa aagaactgat atttaaaaag ctaaaagc
//
LOCUS       DAOSMY010000059         5732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14193_length_5732_cov_2.664435, whole genome shotgun sequence.
ACCESSION   DAOSMY010000059 DAOSMY010000000
VERSION     DAOSMY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14193_length_5732_cov_2.664435"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLD76_02280"
     CDS             complement(<1..269)
                     /locus_tag="PLD76_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU26866.1"
                     /translation="MALKKRNKVNASFSMSSMTDLVFLLLIFFMITSTVVTPSAIKIL
                     LPQSNNQTVAKPITRVIIDENLNYYIATGREKERAVDFSEIEPYL"
     gene            complement(309..1010)
                     /locus_tag="PLD76_02285"
     CDS             complement(309..1010)
                     /locus_tag="PLD76_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU26867.1"
                     /translation="MNLTSILLQVVNNVANTPEVLAETPQVAEMNLFEMALKGGWIMV
                     PIVLLSILAGYIFIERLLIIKKASEEDKTFMNRIKDYILEGKIESATKLCQQNDTPSA
                     RMIEKGISRLGKPMNDVLVAIENVGNIETNKLEKGFAMLATISGGAPMLGFLGTVMGM
                     VRAFFDMANAGSNVDITILSNGIYTAMITTVAGLIVGILAYFAYNYLVSRLDSVVQKM
                     ELRTMEFMDLLNEPI"
     gene            complement(1044..1757)
                     /locus_tag="PLD76_02290"
     CDS             complement(1044..1757)
                     /locus_tag="PLD76_02290"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOU26868.1"
                     /translation="MTKLSVNINKVATIRNSRGGNLPNVLQVALDCERYGAEGITVHP
                     RPDERHIRYSDVLELKPALKTEFNIEGYPQQSFMDLVLRIKPAQVTLVPDPPQALTSS
                     EGWDTIEYQQFLTDIVSTLQKEGIRTSIFVGTNPAHSEYAAKTGTDRVEFYTGPYAHD
                     FIIDKRKAIAPFIKAAEVANKNGLDINAGHDLNLENLHYFFTHIPNLKEVSIGHALIS
                     DALYLGLEKTIRYYKNLLQ"
     gene            1904..2791
                     /locus_tag="PLD76_02295"
     CDS             1904..2791
                     /locus_tag="PLD76_02295"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOU26869.1"
                     /translation="MKVIVLGNIDKTDFLYTTNELLCALEKYKVEILVGEIEYNYLFA
                     NKQYLPNSVSVYSPNQDVDADLAFSLGGDGTFLTSASIIGNKLIPIMGINMGRLGFLA
                     DVPNNSIAEMVDEIFSKGYKTENRTLLKLSTSSKDIFQDDTALNEIAVLKLDSSSMIM
                     IHATINKEYFCSYQADGLILSTPTGSTAYALSVGASILDPTNQSFILAPVAPHSLNLR
                     PFIFPDTWEVDLEIESRNNSFLVSIDGRSYMMDQATKLNIKKAPYSIIIAKRLEDTYF
                     QTLRNKLMWGADTRHKKPF"
     gene            2793..3098
                     /locus_tag="PLD76_02300"
     CDS             2793..3098
                     /locus_tag="PLD76_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HOU26870.1"
                     /translation="MLAYNITFTMDYNVVNFWEQWIVQKFFPMMLETSLHSPKIFKVY
                     TNATEQRIFTVQFLVESFSDYDSFQKDNEAAIIKQLAKTFGNDVVCFTSFIQQISLE"
     gene            3099..3677
                     /gene="ruvC"
                     /locus_tag="PLD76_02305"
     CDS             3099..3677
                     /gene="ruvC"
                     /locus_tag="PLD76_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU26871.1"
                     /translation="MSDSLTYKDAIILGIDPGTTIMGYGLLQIKGGKPTMLAIGVLDL
                     KKYPDHYSKLQRIFARVLSLVDEFKPTALAIEAPFFGKNVQSMLKLGRAQGAAIAAAL
                     YRDIPVHEYAPLKIKMAITGSGAACKEQVARMLQRFLNLSEQDMSLQLDATDGLAAAY
                     CHFLQLNKPFSSSKKTSWKAFAQQNSHRVKGL"
     gene            complement(3682..4863)
                     /locus_tag="PLD76_02310"
     CDS             complement(3682..4863)
                     /locus_tag="PLD76_02310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOU26872.1"
                     /translation="MYKQIFLKPKKEESLKRFHPWVFSGAISHTDKPVEEGEIVEIYD
                     SAKNFIALGHSQIGSIAVRVLSFEKEEINENFWVKRIRESASARTSLGVSHSTTHSIY
                     RLVHGEGDLLPGLIVDVYGKTAVMQAHSIGMHVHRHAIADAIIHVLSPNVSAVYYKSS
                     GTLPYKADIDVEDGFLVGNSTEGTALENGLAFYIDWLKGQKTGFFIDQRDNRSLLEKY
                     ANGKSILNMFCYTGGFSVYALRGGANMVHSVDSSAKAIDLTNKNVTLNFPNDTRHTAF
                     ATDAFKFLAEMDNAYDIIVLDPPAFAKHRDALKNALQGYKRLNAKAIEKIKPGGILFT
                     FSCSQIVSKEQFRLAVFSAAAITGRHVRILHQLTQPADHPISIYHPEGEYLKGLVLMV
                     E"
     gene            complement(4874..5458)
                     /locus_tag="PLD76_02315"
     CDS             complement(4874..5458)
                     /locus_tag="PLD76_02315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU26873.1"
                     /translation="MKHPTHISAELMATLPTLSYPEKKIIIVDTLEKLKEAVNKLNTQ
                     KLIGFDTETKPSFTKGVINKVALLQLASEDVCYLFRLHLIGLPHSLMAILANPSIIKI
                     GIALRDDFSGLRKWNNAKPQGFIDIQQEIHKMGIQELSLQKIYAILFARRISKNQRLS
                     NWESEELSESQQHYAAIDAWACLDIYHEIKTYIE"
     gene            complement(5487..5702)
                     /locus_tag="PLD76_02320"
                     /pseudo
     CDS             complement(5487..5702)
                     /locus_tag="PLD76_02320"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
BASE COUNT         1772 a          979 c          990 g         1991 t
ORIGIN      
        1 tgcagatatg gctcaatctc tgaaaaatca acagcacgtt ctttttctct acccgttgct
       61 atgtagtaat ttaagttttc atcaataatt actcgtgtaa ttggttttgc aactgtttga
      121 ttattacttt gtggtagcaa tatcttaatt gcactaggtg taacaaccgt tgaagtaatc
      181 atgaaaaata tgagtaataa aaacaccaaa tctgtcattg aagacatgct aaatgatgca
      241 tttactttat tccgtttttt taatgccata acctatatga tttatgcaaa tggatgtata
      301 tatttttttt aaataggctc atttaataaa tccataaatt ccatggtgcg taactccatc
      361 ttttgaacaa cagagtctaa tctagaaact aagtagttgt atgcaaaata agctaagata
      421 ccaacaatta atcctgcaac agtcgtaatc atagcggtat agataccatt agatagaata
      481 gtaatatcaa cattgctacc agcatttgcc atgtcgaaaa aggcacgaac catacccatt
      541 acagttccca aaaatcccaa cataggagca ccccccgaaa tggtagcaag catagcaaat
      601 cctttttcta gtttgtttgt ttcaatgttt ccaacgtttt caatggctac taacacgtca
      661 ttcattggct tgcctaaacg cgagataccc ttttcaatca ttcgagcaga aggagtgtca
      721 ttttgttggc aaagttttgt tgccgattca attttgcctt ccaagatata gtctttaatt
      781 ctattcataa aggttttatc ttcctctgat gcttttttta ttataagtaa tctttcaata
      841 aaaatgtaac ctgctaatat agaaaggagt acaataggta ccattatcca tccacctttc
      901 aaagccattt caaataaatt catttcggct acttgaggag tttcagcgag gacttctgga
      961 gtattggcaa cattgttaac aacttgaagt aaaatacttg taaggttcat ggtgttgagg
     1021 attttaaatt gttagtagtc tatttattgt aagaggtttt tatagtagcg aattgttttt
     1081 tctaaaccta aatataaggc gtcagaaatc aatgcgtgcc caatggatac ttcctttagg
     1141 tttggtatat gtgtgaagaa atagtgtaaa ttttctaaat ttaaatcatg accagcatta
     1201 atatccaagc catttttgtt tgctacttct gccgctttta taaatggtgc tattgctttg
     1261 cgtttgtcga ttataaaatc atgtgcatac ggacctgtat agaactctac tctgtctgtt
     1321 cccgttttag ctgcatattc tgagtgagct gggttagtcc ctacgaaaat agatgttcta
     1381 atgccttctt tttgtagtgt tgatactata tccgttaaaa attgctggta ttctattgtg
     1441 tcccatcctt cgctagaagt aagggcttgg gggggatctg gcactaatgt aacttgtgct
     1501 ggttttatac gcagtactaa gtccataaat gattgttgtg gatatccttc aatattaaac
     1561 tcggttttaa gagcaggttt aagctctaac acatcactat atcgtatatg acgttcatca
     1621 gggcgtggat gaacggtaat accctcggct ccatatcgtt cgcagtccaa tgctacttgt
     1681 aacacatttg gcaaattacc accacgggag ttacgtatgg tagctacttt gtttatattt
     1741 acgctaagtt ttgtcattta cttgtaaaaa atcatttttt ttgctgatat ctcaaaacaa
     1801 atgtgttatt ttgcatacca gtctggtatt aaacgcacaa aattacggtt ttattgcgta
     1861 aattcatact gtgaatgatt aaaaaaataa aatttcctgc aaaatgaaag taattgtatt
     1921 agggaacatt gataaaacag actttctata cacaaccaat gagttattgt gtgccttaga
     1981 aaaatataaa gtggaaatac ttgtcggtga aatcgagtat aattatttgt tcgccaacaa
     2041 acaatattta ccaaattcag tttcagtata ttcgcctaat caagatgttg atgctgattt
     2101 ggcatttagt ttgggtgggg atggtacatt cttaacttca gcttctatta ttggaaataa
     2161 attgatacct ataatgggta ttaatatggg tcgtttgggc tttttggctg atgtccctaa
     2221 taattcgatt gctgaaatgg tggatgaaat ttttagtaag ggatataaaa ctgagaatag
     2281 aacgttgtta aaattgtcaa cttcatctaa agatattttt caggatgata ccgcattaaa
     2341 tgaaattgct gtattaaaat tagactcatc atcaatgatt atgatacatg caacgattaa
     2401 taaagaatat ttttgttcgt atcaagcaga tgggttaatt ctttctacgc caactggatc
     2461 gacagcctat gcattgagtg taggagcttc tattttagac cctactaatc agagttttat
     2521 actagcacca gtggctcctc atagtttaaa tttacgtccc tttattttcc ctgatacatg
     2581 ggaggtagat ttggaaatag aaagtagaaa taatagtttt ttagtatcta ttgatggtcg
     2641 ttcgtatatg atggatcaag ctactaagct gaatattaaa aaagctcctt attctattat
     2701 aatagcaaaa agattggaag atacttattt tcaaactttg cgaaataaat taatgtgggg
     2761 agcagatact cgtcataaaa aaccttttta gcatgttagc ctataatatt acatttacaa
     2821 tggattacaa cgttgttaat ttttgggaac aatggattgt ccaaaaattt tttcctatga
     2881 tgcttgaaac gtctttacat tcaccaaaaa ttttcaaagt ttataccaat gcaacagaac
     2941 aacgcatttt taccgtgcaa ttccttgtag agtctttttc tgattatgat agtttccaaa
     3001 aagataatga ggctgcgata atcaaacaat tggcaaaaac atttggtaat gatgtagtat
     3061 gttttacgtc ttttattcag caaatatcat tagagtaaat gtcagacagt ttaacatata
     3121 aagatgccat tattttagga attgacccag gtactactat tatggggtat ggtttgttac
     3181 aaattaaagg tggtaaacca acgatgcttg ctattggtgt gttagattta aaaaaatacc
     3241 cagatcatta ttctaaattg caacgtattt ttgctcgtgt attatcttta gtggatgagt
     3301 ttaaaccaac tgcattagct atagaggctc ctttttttgg taaaaatgtg caatcaatgc
     3361 tcaaattagg tagagcccaa ggagccgcaa tagcagcagc tttgtatcgc gacattccag
     3421 tacatgaata tgctccatta aaaataaaaa tggctataac aggttcggga gcagcatgta
     3481 aagagcaggt tgctagaatg ttacagcgtt ttttaaactt gtcagaacaa gatatgtcgc
     3541 tacaattaga tgcaactgat ggtttagctg cagcttattg tcatttttta caactcaaca
     3601 aacccttttc ttcttctaaa aaaacatctt ggaaggcttt tgctcaacaa aattcacata
     3661 gagtaaaagg cttataactg tctattctac catcaaaacc aatcctttaa gatattctcc
     3721 ttcaggatga tagatgctaa tggggtggtc agctggttgg gttaattgat gtaaaatacg
     3781 cacatgtcta cctgtaatag cagcagcact aaataccgct aatctaaatt gttctttgct
     3841 cacaatttgt gaacaagaaa aagtaaacaa tatgcctcct ggtttaattt tttcaattgc
     3901 ttttgcattt aggcgtttat agccttgtaa ggcatttttt aatgcatctc ggtgtttggc
     3961 aaacgctggg gggtcaagaa caatgatatc atacgcatta tccatttcag ctagaaactt
     4021 aaatgcatct gtggcaaaag ctgtatgacg agtatcgttt gggaaattta gcgttacgtt
     4081 tttatttgtt aagtctattg ccttagctga actatcaacc gaatgtacca tgtttgcacc
     4141 tcctcttaaa gcatatacag aaaaaccccc tgtatagcaa aacatgttta aaatagattt
     4201 gccatttgcg tatttttcaa gcaaagagcg attatctcgc tgatcgataa aaaagcctgt
     4261 tttttgacct ttcagccagt caatataaaa tgctaatccg ttttctaatg cagtgccttc
     4321 tgttgaattg ccaaccaaaa atccatcttc tacatcaata tcagctttgt agggtaatgt
     4381 tcctgacgat ttataataaa cagcactgac gtttggagac aatacatgga taatggcatc
     4441 cgctatggca tgtctatgta cgtgcattcc tatagaatga gcctgcatta cggctgtttt
     4501 tccgtaaaca tctactatta agcctggaag caaatcccct tctccatgca ctagtcgata
     4561 tattgagtga gttgtgctgt gcgaaacacc caacgaggta cgtgcagaag cactttcacg
     4621 aatgcgtttt acccaaaaat tttcattaat ttcttctttt tcaaaagaca gtacacgaac
     4681 tgcaatgctt ccaatttggc tatgacctaa cgcaataaag tttttcgctg aatcataaat
     4741 ttctacaatc tctccttctt caacaggctt gtcggtatga gaaatagccc ctgaaaatac
     4801 ccaaggatga aaacgtttta atgattcttc tttctttggt tttaaaaata tctgtttgta
     4861 cataagctct attttattcg atataagttt taatttcgtg ataaatatcc aagcatgccc
     4921 atgcgtctat ggctgcatag tgttgttgtg attccgacaa ttcttccgac tcccaattgg
     4981 atagtcgttg atttttcgaa attctacgag caaataagat ggcatatatt ttttgcaaac
     5041 taagttcttg gatacccatc ttatgaatct cttgttgaat atcaataaaa ccttgaggtt
     5101 tagcgttgtt ccatttgcgt aatccactaa aatcatcacg taatgctatc ccaattttta
     5161 taatagatgg attagctaaa atagccatta aagagtgggg taatccaatg aggtgtaagc
     5221 gaaataaata acaaacatct tcagaggcta attgtagtaa tgctacttta ttgattactc
     5281 cttttgtaaa ggatggtttc gtttctgtgt caaaacctat taatttctga gtatttaatt
     5341 tatttacagc ttcttttaat ttttctagcg tatcaacgat gataattttt ttttcggggt
     5401 aagaaagtgt gggaagggtt gccattaatt cggctgaaat atgtgtcgga tgtttcatct
     5461 tacattcact tattaaggat aaaaacttac aataaattgc tgtagtcatc ttcttcatca
     5521 cgcaaaaaat cgctaaaagt ctgttttttt accgtatttt tttgtaatat agactcatct
     5581 gttacatttt ctaattcatt attgtataaa atactgtgaa tctgtttcag tgcactaagc
     5641 aacttttgac cccagtattg ctgaaagttt tctatacata tttttacagc aatacttact
     5701 acttcaggat ttcctactta gcattcatta ct
//
LOCUS       DAOSMY010000060         5723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14220_length_5723_cov_3.830981, whole genome shotgun sequence.
ACCESSION   DAOSMY010000060 DAOSMY010000000
VERSION     DAOSMY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14220_length_5723_cov_3.830981"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="queF"
                     /locus_tag="PLD76_02325"
     CDS             complement(<1..310)
                     /gene="queF"
                     /locus_tag="PLD76_02325"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOU26874.1"
                     /translation="MNTPQFTHLGKKSNTPTKQLDTFPTPKNVEIVKFESDELTSFCP
                     VTHQPDFNRVIIEFSPDAYCIESKSLKLYLWSFREEAKFAEALAGEIADDIFNAIQPH
                     W"
     gene            complement(323..745)
                     /locus_tag="PLD76_02330"
     CDS             complement(323..745)
                     /locus_tag="PLD76_02330"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOU26875.1"
                     /translation="MQYVITKEFTFEAAHRLLRNYCGKCSNNHGHSYRVKLHLQGTNL
                     DEKDMLIDFNETKKLKAWIDDNLDHVTMLWEEDPMIDSMRTFGNKVLVTKKNPTAEHI
                     AELILAEAQRLFENSAIQVKCIEINETCTSAARLYPLS"
     gene            complement(756..1460)
                     /locus_tag="PLD76_02335"
     CDS             complement(756..1460)
                     /locus_tag="PLD76_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOU26876.1"
                     /translation="MKTSPVYYVAEIFESIQGEGNYAGVYSLFIRFQHCNLACSWCDS
                     KYTWYKNDTLIPQTADELKDIIASSTATHIIFTGGEPTLYALDKLVIPGKKYHVETNG
                     TVIPTEPLHIELPDGTYIERVAMKESIIKRFNWVVSPKLSNAINSQNTEYLHYWSARN
                     WGIFKFIIQQSADIDEIEHVINRYSIDKNRVYLAVEGCTLQSQLRPELVDELITKGFH
                     YSPRLHVLLWGAKRKK"
     gene            complement(1467..2159)
                     /gene="queC"
                     /locus_tag="PLD76_02340"
     CDS             complement(1467..2159)
                     /gene="queC"
                     /locus_tag="PLD76_02340"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOU26877.1"
                     /translation="MVASTNKAYILLSGGQDSFVCLIWALQHFSEVEAVSIAYNQRHA
                     KEIEYAKKIAAHFNVPHFTYDIGSFMSSIAESSLLNTTNHNTQHAQASHLPASFVPNR
                     NGLFLTIIANHAFKQETKHIHLVTGTCETDYSGYPDCRDNYIKAKATELSLGLDISVS
                     IHTPLMWKTKAETFAMAHEAGKLNELIQLTLTCYNGEETMHPWGYGCGECPSCVLRKK
                     GYEEFLHLTYTV"
     gene            complement(2174..3889)
                     /locus_tag="PLD76_02345"
     CDS             complement(2174..3889)
                     /locus_tag="PLD76_02345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU26878.1"
                     /translation="MDNYIVSARKYRPITFDSVIGQHALVTTLKNAIKNKQLAHAYLF
                     CGPRGVGKTTTARIFAKTINCLQPLPNHEACNQCESCMAFNEQRSFNIHESDAASNNS
                     VDDIRSLIDQVRIPPQIGKYSVYIIDEVHMLSQSAFNAFLKTLEEPPAHAIFILATTE
                     KHKILPTILSRCQIYDFNRISVQDTVTHLQHIASLEGITAEPEALHVIAHKSDGGMRD
                     ALSTFDQVVSFCGNTITYKHVIENLNILDYDYYFKLVDAFLSGNVPASLLLFNEILNK
                     GFDAHNFIVGLSEHLRDVLVCKDERTAILLEVGASIRESYIQQAKACTDAFLYEALSL
                     SNACDLEYKQSKNKRLLIELCLIKLCQILTPKVAQQQGSTPLQVIQTNTTQPQATPIQ
                     KEASKLVATPTYTTPLTKSTPPSTPISTSSVGSVSIKQISSSVSQQTQTIQPAKERTL
                     REEPFTQVQLEEVWRKFGNEIPDRHKMLFLFSSKKPKQIAKSSIELEVNDPLLVKDIE
                     DIKPQLMAYLTQQLQNDKISLLITLVPLTAEHKAISNVDKIRLLKKKRPEFENMIQQL
                     GLDPE"
     gene            4161..4457
                     /locus_tag="PLD76_02350"
     CDS             4161..4457
                     /locus_tag="PLD76_02350"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU26879.1"
                     /translation="MKKIVDFFITYILNKYVIASAVFVFVMFTNEDYNIKTRYANAQK
                     IEKLEAEIRYYQEETKKNKEKLDLLQSDKENLERFAREQFIMKKDSEDVFVVVD"
     gene            4468..4782
                     /locus_tag="PLD76_02355"
     CDS             4468..4782
                     /locus_tag="PLD76_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26880.1"
                     /translation="MKLLYIISFLAVLVATILYMFFPQVAPFVFSVGVVGIIVFRLRT
                     LYAGSDFRVKRLYIMQNLATLLYIAVAYFMFIQSDIWVFILLVAALVETMVVLRMPNK
                     RE"
BASE COUNT         1672 a          937 c         1076 g         2038 t
ORIGIN      
        1 accaatgtgg ttgtatagca ttgaaaatat cgtctgcaat ttcgcctgct aatgcttcgg
       61 caaatttggc ttcttcgcga aatgaccaca aatagagttt caaactttta ctttcgatac
      121 agtaggcatc gggagaaaat tcgatgataa cacgattaaa atctggttga tgtgttacag
      181 gacaaaaact ggttaattca tcgctttcaa attttacaat ttctacgttt ttgggtgttg
      241 gaaacgtatc gagttgtttg gtaggtgtgt tgcttttttt gcctaaatgt gtaaattgag
      301 gtgtattcat ggtgttattc ttttaagata aagggtataa acgtgccgca gatgtacagg
      361 tttcatttat ttcaatacat tttacttgta tggcagagtt ctcaaacagt cgttgtgctt
      421 ctgctaaaat gagttcggca atatgctcag ccgtaggatt cttttttgta actagtactt
      481 tgttgccaaa cgtacgcata ctatcaatca tcggatcttc ttcccagagc atggttacgt
      541 ggtcaagatt gtcgtctatc catgctttta atttttttgt ttcgttaaaa tcgataagca
      601 tgtctttttc atccaagtta gtaccttgta agtgcaattt tacgcgatac gaatgaccgt
      661 gattattgct acacttgcca caataattgc gtagtaaacg atgagctgct tcaaatgtaa
      721 attctttagt gataacgtat tgcatagttg tatagttatt tttttcgttt ggctccccac
      781 aatagtacat gtaagcgtgg cgaatagtga aaaccttttg ttatcaattc atccaccaat
      841 tcaggtcgca attgcgattg caacgtgcat ccttctactg ctagatatac gcggttttta
      901 tcaatagaat aacgattaat tacgtgttct atttcgtcaa tatctgcaga ttgctgtatg
      961 ataaatttga atatgcccca gttacgagcc gaccaatagt gcaaatattc cgtgttttgg
     1021 ctattaatcg cattgcttag ttttggcgaa actacccaat taaagcgttt gatgatggat
     1081 tctttcatag cgactctctc tatgtatgtg ccatcgggca attcgatatg cagaggctct
     1141 gtggggataa ccgttccatt cgtttctaca tggtattttt taccaggtat tactaactta
     1201 tccaatgcat acaaggtagg ttcacctcct gtaaaaataa tgtgtgtagc tgttgatgag
     1261 gcaattatgt cttttagttc atcggctgtt tgaggtatga gtgtgtcatt tttataccaa
     1321 gtatatttag aatcgcacca agaacatgcc aaattgcagt gttgaaaacg gataaacaag
     1381 ctatacactc ctgcataatt tccttctccc tgaatcgatt caaagatttc ggctacgtaa
     1441 tatacagggc tagttttcat gtgcatttaa acagtgtatg ttagatgtag aaattcttcg
     1501 tatccttttt tacgtaatac acacgaagga cactcaccac atccatatcc ccatgggtgc
     1561 atcgtttctt ctccattata acaggtaagt gtgagctgga taagttcgtt caattttcct
     1621 gcctcatgag ccatagcaaa tgtttcagct tttgttttcc acatcagcgg agtatgtatt
     1681 gaaacagaaa tatccaaacc caatgataat tcagttgctt tagctttgat gtaattgtct
     1741 cgacagtcgg gataacccga ataatctgtt tcgcaggtgc ccgttaccaa atgaatgtgt
     1801 ttggtttctt gcttgaacgc atgattggct ataatcgtta aaaataatcc attgcgattg
     1861 ggtacaaaac tcgcaggtaa gtgcgacgct tgtgcgtgct gggtattgtg gttcgttgta
     1921 tttaataaag aactttcggc aatactactc ataaaagaac caatatcata cgtaaaatga
     1981 ggtacgttaa aatgagctgc tatttttttt gcgtattcta tttcttttgc atgacgttgg
     2041 ttatacgcaa tactaacagc ttccacttca ctgaaatgtt gtaaagccca aattaagcaa
     2101 acaaaagaat cttgcccgcc cgaaagcaat atatacgctt tatttgtaga tgctaccata
     2161 actgtgtttt tatttattca ggatctagtc ctaattgctg tatcatattc tcaaactcgg
     2221 gacgtttttt tttcaataaa cgtattttgt ccacattgct tatggctttg tgttctgcag
     2281 tgagtggtac caatgttatg agcaaactta tcttgtcatt ctgtagttgt tgagtcaaat
     2341 acgccattaa ttgaggctta atgtcttcga tatcttttac taacagaggg tcattcactt
     2401 ccaattcaat actcgatttt gctatttgtt ttggcttctt gcttgaaaac aaaaacaaca
     2461 ttttatgtct atctggaatt tcgttaccaa attttcgcca aacttcttct aattgcactt
     2521 gtgtaaatgg ttcttcacgc agtgttcttt cctttgcagg ttgtatggtt tgtgtttgtt
     2581 gactcacaga tgagctaatc tgtttgatag aaacactacc aacagaagat gtactaatag
     2641 gggttgatgg aggtgtactt ttggtaagag gtgtggtata agtgggtgtg gcaaccagct
     2701 tgctagcttc cttttgtatg ggagtcgctt gcggttgcgt tgtgtttgtt tgaataactt
     2761 gtaatggtgt gctgccctgt tgttgtgcta ctttgggtgt gagtatctga cacaatttaa
     2821 taagacataa ttctattaat aaacgtttat ttttgctttg tttgtactct aaatcacagg
     2881 cattgctcaa cgataaagct tcgtataaaa aagcatctgt gcatgctttt gcttgttgta
     2941 tatacgattc cctaatgcta gcacctactt ctaacaatat ggctgtacgc tcatctttgc
     3001 atacaagtac atcacgcaaa tgttccgata atcctacaat aaaattgtgt gcatcaaaac
     3061 ctttgttcaa aatttcatta aataagagca aacttgccgg tacatttcct gataaaaaag
     3121 catctaccaa tttaaaatag tagtcgtaat ccaatatatt taggttttca atcacgtgtt
     3181 tgtatgtaat tgtgtttcca caaaaactga caacttgatc aaaagtagaa agtgcatcac
     3241 gcataccacc gtccgatttg tgtgcaataa cgtgtaaagc ttcaggctcg gcagtaatcc
     3301 cttctaaaga ggctatgtgt tgcaaatgag ttacggtatc ttgtacgcta atgcgattaa
     3361 aatcataaat ctgacaacga gataaaatcg ttggtaaaat tttgtgtttt tctgtcgtag
     3421 ccaagataaa tatggcatga gcaggaggtt cttctaaggt tttcagaaaa gcgttgaacg
     3481 cactttgcga aagcatgtgt acctcgtcga tgatatatac actgtatttg ccaatttgtg
     3541 gtggtatacg tacttggtct attaatgacc gaatatcatc taccgagtta ttagaggcag
     3601 catccgattc gtggatatta aacgagcgtt gttcgttaaa tgccatacac gattcgcatt
     3661 ggttgcaagc ctcgtggttg ggtaggggtt gcaagcagtt aatcgttttg gcaaaaattc
     3721 gagctgttgt tgtttttcct acacctctag gtccacaaaa caaataagca tgagccaatt
     3781 gcttgttttt aatagcattt ttgagtgtag taaccaatgc atgctgacca ataaccgaat
     3841 cgaatgttat ggggcggtat tttcgtgccg aaacaatgta attatccatt tgcttttttt
     3901 tcctttgcac aaagataaaa aaatgttcat aaatagaacg taaaatttag ctttttaaag
     3961 aaaaaaaact acatttgtag gttgatatta aacttcagtc tgctataatt ggtttacctg
     4021 ttgtcttgtt gtgattgggt tcttttagag gaatcctttg tttgcaggga taaatgaaag
     4081 tagcccatta gtgggaacag ataaagaagc atatatgtat ctcaatttta tttaatatag
     4141 ttacctttta acaatctagt atgaaaaaaa tagtagattt ttttatcaca tatattctta
     4201 acaaatacgt tatagcatct gctgtttttg tttttgtgat gtttaccaac gaagattata
     4261 atatcaaaac aaggtatgcg aacgcacaaa aaatcgaaaa actagaggca gaaatacgct
     4321 attaccaaga agaaacaaag aaaaataaag aaaaactaga tttattgcag tcagataaag
     4381 aaaatttgga acgatttgcc cgtgagcaat ttataatgaa gaaagatagt gaagatgttt
     4441 ttgtggtggt agattaaaat ttgtagtatg aagttgttgt atattatttc ttttttagca
     4501 gtacttgtag ctactatttt gtatatgttt tttccacaag tagctccatt tgttttttcg
     4561 gtaggagttg taggtattat cgtttttcgt ttgcgaacgc tctatgctgg atccgatttt
     4621 agagtaaaac gattatatat aatgcaaaat ttagcaacct tactatatat agcggttgcg
     4681 tattttatgt ttattcaaag tgatatttgg gtattcatat tgctcgtagc agccttggta
     4741 gaaacgatgg ttgtattgcg tatgcccaat aagagggagt aagagcaggg tagacaagtt
     4801 tgcgtatgca ttgatttagt aaacagtgtg caataaaaac taccccgttt tttacaatag
     4861 attgttacaa aacagtggag tacgggaaag ttaacatgta tacgcgttac agcataacga
     4921 tataagtaaa ttttatatag tgtaattgtt gggagtactt accacaaggt ttcgttaagg
     4981 gatttttttt gttttatcga tgttttgcaa aacgtacaga tttatggcaa ttaacccgat
     5041 gcttgcgttg tcaatcagat tctttttaaa aaaacacttc tccattaggt actcttaatg
     5101 taatttttga taagtatcaa atttttctca tcgcaatgat gaaattcaaa atctaaatct
     5161 gtaagtattg ggataaaatg cgatttctca attgggatac taatctacag aagtttttta
     5221 tttgttaaag attgaattag tcgtactatt tcttttttaa attcatcttt gtttggtgga
     5281 agtgttgcat tgttaactat tactccatca tatgcattgt tacttatttc tatcatagat
     5341 gattattttt tttcgatatg attgctaatg ctatctacat tcatagaatc aattctttct
     5401 actatagttg aaagcgaaca taaaaagaat actgacctgt atcttgtgta tcgtttcctt
     5461 ctgtttggat ttttgctata tgttctaact aacccatttt cagttttttt acatcactgt
     5521 tttatcccca aaaaatagag gaaaaattat gaggtacttt ggcaaagata tacattttta
     5581 tacagataaa agttcttatt tgctttaaaa tatctcagaa acgcttttag ctaactatgt
     5641 gaccaaattg tgtgaaaaat agaaagatga tgattcttta caaataaaaa taggtgtttt
     5701 tgtttccatt ttacactttt ttg
//
LOCUS       DAOSMY010000061         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14264_length_5713_cov_3.716508, whole genome shotgun sequence.
ACCESSION   DAOSMY010000061 DAOSMY010000000
VERSION     DAOSMY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14264_length_5713_cov_3.716508"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..914)
                     /gene="fabG"
                     /locus_tag="PLD76_02360"
     CDS             complement(168..914)
                     /gene="fabG"
                     /locus_tag="PLD76_02360"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU26881.1"
                     /translation="MSLLKGKVAVVTGAARGIGKAIALKYASEGADIAITDLAVDDNA
                     KAVEKELAAYGVKAKAYASNAANFDDTHNVVSEILKEFGRIDILVNNAGITRDGLMMR
                     MTEQQWDMVINVNLKSAFNFVHAITPIMMKQKTGSIINMSSVVGVSGNAGQCNYSASK
                     AGMIGLTKSIAKELGSRGVRANAIAPGFIITEMTAALSDEVRDQWAAQIPLRRGGTPE
                     DVANVATFLASDMSSYVSGQTIHCCGGMNM"
     gene            complement(1118..1633)
                     /locus_tag="PLD76_02365"
     CDS             complement(1118..1633)
                     /locus_tag="PLD76_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU26882.1"
                     /translation="MVTFSGIIPVETQWLTIQVVGNGSVKVGDVAYTTAVPVAKDEQV
                     TISAIPNHGNVFVAWSGDLVSTTASETITMTTDKTITVTFDQVTQLTQNEAVSVVLYP
                     SPFTDQLHVQSAEKLDKILVTNAVGVSMYDTTNVDSAITIPTSQWTSGIYIVHMYKAN
                     KVIATKKVVKF"
     gene            complement(1682..1840)
                     /locus_tag="PLD76_02370"
                     /pseudo
     CDS             complement(1682..1840)
                     /locus_tag="PLD76_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
     gene            complement(1877..2092)
                     /locus_tag="PLD76_02375"
     CDS             complement(1877..2092)
                     /locus_tag="PLD76_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26883.1"
                     /translation="MQHATFFPVVTDSLLPYIDSFSYVSFDSNDSLLVPPLGFPVILF
                     CFSPHNSFYTHNEFKNESVVVGQMTRW"
     gene            2149..2493
                     /gene="folB"
                     /locus_tag="PLD76_02380"
     CDS             2149..2493
                     /gene="folB"
                     /locus_tag="PLD76_02380"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU26884.1"
                     /translation="MAVINLENMEFWSYHGCFEEERRIGTKYVISVSLAVDIQQAMAS
                     DNLHDTINYQEVYSIIKEEMDKPSNLIEHVAGRIQHRLKERFPQSTQQTISLSKFNPP
                     LGGQIEKVTIVL"
     gene            2638..3198
                     /locus_tag="PLD76_02385"
     CDS             2638..3198
                     /locus_tag="PLD76_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26885.1"
                     /translation="MKFGSILVIALIFLTSCATQRTSTIVGCDYDKNKNQTEYFVFPY
                     GSVSIPGEWKKTTYNQIAKQQFFRNPDSITIAIAFGPCDKYEFNATNPMKGFEFVKAF
                     YEWDSDFFVKNYRLNQELVESNESENFIIWRAFGENNNTYWDTYFLFGEKNGFANNLS
                     IMTTEKWTKEDKVTFLKKMYLQKKTE"
     gene            complement(3322..5013)
                     /locus_tag="PLD76_02390"
     CDS             complement(3322..5013)
                     /locus_tag="PLD76_02390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU26886.1"
                     /translation="MQKYIFILFFTFHVFSISAQFDKTEYDLAFPLNIPVELSGNFGE
                     LRSNHFHAGIDFKTQQVIDKPVFSVADGYVSRFGVSPTGYGHVLYITHYNGLTSVYAH
                     LDSFVPFLDSIVQAKQHEERSFAVDFTLPKDMIKVKRLQQVGWSGNTGSSGGPHLHFE
                     LRDTYTEELLNPFLFYDIEDTVRPRVHKFLLYPSSNEGVVEGSMRRRTMVVEQTKPGF
                     YRHKGNVTIKAWGKVGLGVHSYDYMPNANNIYGVHSVRVLVDGAEIFVYQMDRFSFDK
                     TRYMNAFIDYEEWYTNRSMVMTLFRLPNNKLDNYVKLVNDGYLLINEERIYPVQLIIK
                     DFHGNTTTLDFSIQGVKQEIPKVEHPNAVAFSYDKENVFENADVKLTIPAGALYQNML
                     FNYSVRYNPKGFSDVHDLHLPTIPLHDYAELRIKLKNDSLSDKNKYYIARKNRLNGFD
                     FVGNGYEDGCVVTKTRDFGQYAVLLDNEAPIIRLATVNNGHVIFKVSDNQSGVSHYNG
                     YVDNEWALFKYDKKTNTITHKFDSKRVEKGGIHHIELRVFDKCGNEAVYQNQVWW"
     gene            5235..5495
                     /locus_tag="PLD76_02395"
     CDS             5235..5495
                     /locus_tag="PLD76_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOU26887.1"
                     /translation="MKKGIHPENYRLVVFKDMSNGDTFLTRSVAPSKETIEFEGATYP
                     VIKIDISNTSHPFYTGKSTLVDTAGRVDKFKSRYQKHIDKKK"
BASE COUNT         1961 a         1037 c          908 g         1807 t
ORIGIN      
        1 tgtgtatttt atcaccacgt attttatgtt taaacaaaaa aataactatt ttttaccctc
       61 tatgccatta ttaaacaaat ccatccaata aaaattgctt cttctgattt cgaaaaatat
      121 ctctatcaaa agaagcaata aggtgttatc aggaaattgg aaaaatccta catattcata
      181 ccaccacagc agtgaatagt ttgcccagat acatagctag acatatccga agccaagaaa
      241 gtagctacat tcgccacatc ttcgggtgta ccgcctctac gcaatggaat ttgtgcagcc
      301 cattggtctc ttacctcatc cgaaagggca gctgtcattt cggtaataat aaatccaggt
      361 gcaatggcat tggctctaac acctcgcgag cccaattctt tggctatcga tttggttaaa
      421 ccaatcatac ctgctttgga tgcagaatag ttacattgtc cagcattacc acttacgcct
      481 actactgacg acatgttgat gattgaacct gttttttgtt tcatcataat aggtgtgatg
      541 gcatgtacaa agttgaaagc tgatttcaaa tttacattga ttaccatatc ccattgttgc
      601 tcagtcatac gcatcatcaa accatcgcgg gtgatacctg cattatttac taaaatatca
      661 atacgtccaa attcttttaa aatttcagat accacattgt gtgtatcgtc aaaattggca
      721 gcattagacg catacgcttt ggctttaaca ccataagcag ctaattcttt ttctacggct
      781 ttggcattgt catctacagc taaatctgtg atggctatat ctgctccttc tgaagcgtat
      841 ttcaatgcaa tagctttacc aataccacgt gcagctcctg ttactacggc tacttttcct
      901 tttaataaac tcataaatgt atgtttttta ttagatttaa cggatgcaaa tgtacgcatt
      961 ttatcagaaa tcttatacat tttatataaa aaagggggaa atgtgatgtt gcagattact
     1021 ataaacaata atttttacgc tacatagtat tgttataaaa cacaaagagg caagatttct
     1081 ctcgcctctt tatgctaaaa aaacagatag atgtttttta gaattttacc acttttttag
     1141 tggctattac tttgttagcc ttgtacatat gtacaatgta aataccactt gtccattgag
     1201 atgttggtat ggtaatggca ctatcaacat tcgttgtatc atacatcgaa acccctacag
     1261 cattggtaac taatatttta tctaattttt cagccgactg tacatgaagc tgatctgtaa
     1321 acggacttgg atacaaaact actgatacag cctcgttttg tgttagttgg gttacttgat
     1381 caaaagtaac tgtaatagtt ttatccgttg tcattgttat agtttctgaa gctgtagttg
     1441 ataccaaatc gccactccat gccacaaaca cattcccatg attaggaatg gccgaaatgg
     1501 ttacttgctc atccttggct acaggcacag cagtagtata agctacatca ccaactttta
     1561 ctgacccatt tccaacaact tgtatggtca accattgggt ttcaactggg ataattccag
     1621 aaaatgtaac catataatca ttgttggtaa aatgagtaat tgtattatct acattaatta
     1681 gtcgtggttg taaaagtgga aatcctgtct tttcaatcaa tatggcataa tccccaggag
     1741 gaactccatt aaaattgtaa tacccatttt catctgtcag agtctgagag ataatctgat
     1801 tgttggcaac atcaaacaaa tatacaagta attgttcaag aggattattg aaggctctct
     1861 tattttatgg cacttactac caacgggtca tttgccctac taccaccgac tcatttttaa
     1921 actcgttgtg cgtataaaaa gaattatgag gcgaaaaaca aaacaaaata acaggaaagc
     1981 ctaagggtgg aactaagagg gagtcgtttg aatcaaaaga aacatacgaa aaactatcaa
     2041 tgtagggtaa caatgagtct gtaactacag ggaagaatgt tgcatgttgc attcaattat
     2101 ttttttacaa aaatagaaaa atttatttaa ctttgtaaaa aaataaatat ggcagttatc
     2161 aacttagaaa atatggagtt ttggtcttat catgggtgtt ttgaagaaga acgtcgcata
     2221 ggcaccaaat acgtaataag tgtatcgtta gcagtagata tccaacaagc aatggcatca
     2281 gacaacttac acgacacgat taactaccaa gaagtgtatt cgataataaa agaagaaatg
     2341 gacaaaccat caaaccttat cgaacacgta gcgggcagaa tacaacacag actaaaagaa
     2401 cgtttcccac aaagcactca acaaacaatt tctttatcca aattcaatcc tcccttaggc
     2461 ggtcaaattg aaaaagttac cattgttttg taatggttgt aactacttac gctgagggtg
     2521 atattttttc tttgtttcat tttgagcaat gcttgtattt aggggaattt gtgtacattt
     2581 gtgagttggc agaaaattta caaagacgat ataaacaaca gaacattaat taattaaatg
     2641 aaatttggaa gtatattagt tattgcactt atctttttga caagttgtgc tacacaaagg
     2701 acaagtacta ttgtaggatg tgactatgat aaaaataaaa accaaacgga atattttgtt
     2761 tttccatatg ggtctgtttc tattccagga gaatggaaaa aaacaacata taatcaaata
     2821 gcaaaacaac aattctttag aaatccagat agtataacaa tagcaattgc atttggacct
     2881 tgtgataaat acgagtttaa tgcgactaat cccatgaaag gttttgaatt tgttaaagca
     2941 ttctatgaat gggactccga tttttttgta aaaaattatc gtttgaatca agaattagtt
     3001 gaatctaacg aaagtgaaaa ttttattata tggagagcat ttggagaaaa taataataca
     3061 tattgggata cttatttttt atttggagaa aaaaatggtt ttgcaaataa tctttctatt
     3121 atgactaccg aaaaatggac aaaagaagat aaagtgacct ttttaaagaa aatgtattta
     3181 cagaagaaaa cagaataaat ccccatcgct ggtgcaggtc tgagacctgt gcctcatatt
     3241 caatacccca tcaggtcaga attttcaatc cgcttaataa ttcaaaaaga aaaagttatt
     3301 ctttctccta acgaggtgat actaccacca aacctgattt tgatatacag cctcgttgcc
     3361 acatttatca aacacacgta actctatgtg atgaataccc cctttttcca cacgcttact
     3421 atcaaactta tgtgtgatgg tattcgtttt tttgtcgtat ttaaacaatg cccattcatt
     3481 atctacatac ccattataat gagatactcc tgactgatta tcacttacct taaatattac
     3541 atgaccatta ttgacagtgg ctaatcgaat aataggagct tcattatcta acaaaacagc
     3601 atattgtcca aagtcacgag ttttggtaac aacacagccg tcctcatacc catttccaac
     3661 aaaatcaaat ccatttaagc gatttttgcg agctatataa tatttatttt tatccgacaa
     3721 actatcattt tttaatttta tacgcaattc tgcatagtca tgtagcggta tggtaggcaa
     3781 atgtaaatca tgtacatcag aaaatccttt tggattatat cttacggaat aattaaataa
     3841 catattttga tacaacgcac ctgctggtat cgttaattta acatcagcat tttcaaatac
     3901 attttcctta tcatacgaaa aagcaaccgc attcggatgt tccactttag gtatctcctg
     3961 ttttactccc tgaatggaga aatctaacgt ggtagtattt ccatgaaaat ctttgataat
     4021 caattgtact ggatatatgc gttcttcatt aattaacaaa tagccatcgt ttactaactt
     4081 cacataatta tcaagtttgt tattgggcaa acggaataaa gtcattacca tagaacgatt
     4141 tgtgtaccat tcctcataat caataaaagc attcatatac ctagttttat caaacgaaaa
     4201 tctatccatt tggtatacaa atatttctgc tccatcaact aaaacacgta cagaatgaac
     4261 tccatatatg ttattggcat taggcatgta atcataagaa tgtacgccta atcctacttt
     4321 tccccatgct ttgatggtta cattgccctt atgtcgataa aatcctggct tagtttgctc
     4381 cacaaccatt gttcgcctac gcatagaccc ttctacaaca ccttcgttag atgacggata
     4441 taacaaaaat ttatgcacac gtggacgcac ggtatcttct atatcataaa acaaaaaggg
     4501 attcagtagc tcttctgtat atgtgtctct caactcaaaa tgcaaatgtg gtcccccaga
     4561 actgcctgta ttaccactcc atcccacttg ttgcaagcgt tttactttaa tcatatcctt
     4621 aggtaaggta aaatcaacag caaacgaacg ttcctcatgt tgtttggctt gtacaattga
     4681 atctaaaaat ggcacaaagc tatccaaatg agcatacact gaagtcaatc cattatagtg
     4741 agttatatac aatacatgac cataaccagt gggcgatacg ccaaaacgag aaacataccc
     4801 atcagccact gaaaaaacag gcttatctat tacttgttgt gttttaaaat ctatacccgc
     4861 atgaaaatga ttagaacgta actctccaaa attaccactt agttcaacag gtatatttaa
     4921 agggaaagct aaatcgtact ctgttttatc aaactgtgct gatatagaaa aaacatgaaa
     4981 agtaaaaaag agaataaaaa tatatttttg cattgtatag ggataattta tgagcacaaa
     5041 aataaggata aaaaaagaaa gaacagcgtt aatcattaaa aaaacaagct tttttcaaaa
     5101 cgctacagac tttttttctc aaaaaaaaag atttatagta aaaaaggaag attttgtttt
     5161 gcaaatttga ttcttatttc taactttgca gtccgaaata attttgaagg aacaatatta
     5221 aacgtacata taaaatgaaa aaaggaattc accccgaaaa ttaccgtttg gttgtattta
     5281 aagatatgtc taatggagac acatttctta ctcgttcggt agcaccatct aaagaaacta
     5341 ttgaatttga aggagcaaca tatccagtaa ttaaaattga tatttcaaat acatctcacc
     5401 ctttttacac aggcaaatct actttggtgg atacagcagg tcgcgtagat aaatttaaat
     5461 cacgttacca aaaacacatt gataagaaaa aataagttag agattatttt ttatattttc
     5521 attacaaacc ctgaaacaaa ctttcagggt tttgttatat aatagccata aatggaaaga
     5581 gactgtcttt gatttctcag acagtctctt tttaagaaga aaaatactat gaacgttcgc
     5641 cgatatctga cgttctccgt agcgatagcg aaggagaatg tggcgaagcc cttgcagata
     5701 tcggctgttg tgt
//
LOCUS       DAOSMY010000062         5697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14317_length_5697_cov_3.281815, whole genome shotgun sequence.
ACCESSION   DAOSMY010000062 DAOSMY010000000
VERSION     DAOSMY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14317_length_5697_cov_3.281815"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /gene="gyrB"
                     /locus_tag="PLD76_02400"
     CDS             complement(<1..1896)
                     /gene="gyrB"
                     /locus_tag="PLD76_02400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU26888.1"
                     /translation="MSEEEKKENGGYGASSITVLEGLEAVRMRPAMYIGDIGTKGLHH
                     LVYEVVDNSIDEALAGYCKHIDVFINEDDSITVKDDGRGIPVDMHEKEGKSALEVVMT
                     VLHAGGKFDKDSYKVSGGLHGVGVSCVNALSDYMRVEVHKGGKVYEQEYSCGKPQFSV
                     REIGVSTQKGTTVTFKPDGSIFMTTEYKYDILAGRLRELAFLNAGIRLILTDKRERFE
                     DGSYKSEEFYSTEGLAEFVRFIDQAREKLIDEVIHISTDKYATPVEIAMLYNTSYNEN
                     IHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEENKLLEKLEKNKIEIDGNDFREGLT
                     AVISIKVAEPQFEGQTKTKLGNNEVMGSVDMAVSEALGNYLEEHPKEARTIVEKVILA
                     ATARHAARKARDLVQRKSPLSGAGLPGKLADCAEKDPAKCELFLVEGDSAGGTAKQGR
                     DRATQAILPLRGKILNVEKAMPHKVFESEEIRNIYTALGVTIGTEEDSKALNLSKLRY
                     HKVVIMTDADVDGSHIATLILTFFFRHMKDLIENGYVYIATPPLYLCRKGKIEEYCWD
                     EKQRLAFVEKYGKGSEGGIHIQRYKGLGEMNAQQLKQTTMDPENRTLRQVTIENAAEA
                     DHIFSMLM"
     gene            2128..3111
                     /locus_tag="PLD76_02405"
     CDS             2128..3111
                     /locus_tag="PLD76_02405"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOU26889.1"
                     /translation="MKKKIAIIAGGNSSEVIVSLKSAQGIYSFIDKKTYEVYIVTLIE
                     KKWSVKLEDETEVDIDKNDFSFILNGEKILFDFAYITIHGTPGENGILQGYFDLIGLP
                     YSCCGVLAAAITFNKFTCNQYLKGFGIPVAPSILLRKNEHIQNTVVVEKIGLPCFIKS
                     NVGGSSFGVTKVKEEKQIQPAIEKAFAEGNEVIIEGFLQGTEVTCGMYKTHEKTQVFP
                     ITEVVSANEFFDFDAKYNGQVTEITPARIPQEVTNHIQQLTNQLYDILGCKGIIRADF
                     IINKQNVPYLLEVNTTPGMTPTSFIPQQVKAAGMAISSVMEDIIENKLNHL"
     gene            3135..4109
                     /locus_tag="PLD76_02410"
     CDS             3135..4109
                     /locus_tag="PLD76_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU26890.1"
                     /translation="MKSREEIIEIVQNELTLTNWLSEPIDLYKPIAYTMSMGGKHIRS
                     AITLMACNLYSDNIHLPLQPAIGIEVFHNFTLLHDDIMDKAETRRGKPTVHVKWNDNT
                     AILSGDAMLIKAYQLLAKCPPENLSEVLSVFSQTAIEVCEGQQYDMNFEHQDTVSEEE
                     YIEMIRLKTAVLLAASLKIGAIIGGATPLQANALYDFGIHIGIAFQLQDDLLDVYGDP
                     TIFGKKIGGDIICNKKTFLLIRALKNANNTQRNKIQHWMTQHPDDPTEKIVQMTSIFN
                     ETGVKQACQQRMKEHYNKALLALNKACEVEEKIVHLQKLASELMQREK"
     gene            4179..4892
                     /locus_tag="PLD76_02415"
     CDS             4179..4892
                     /locus_tag="PLD76_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26891.1"
                     /translation="MPYRRLPNTDAARLRALRAVACYKNSPIDTELPFDRRILQEICS
                     FLPQFENAMFEYKQAINSEGNKNNKYQQYIKNVRLYISHFIQVLNLACIRNEIKPEKK
                     LLYKLLPDNYAVPDLSTETLLIEWGKNIIQGENERTRAGGSPIYNPTIAKVQVHYDIF
                     KEAYHSKKVAQKNVGRYAENMQKMRMRADEIILDAWNQIEQAFVHYGMDEMQQKAKEF
                     GIVYYLRRKERQAMTKKSH"
     gene            5020..5418
                     /locus_tag="PLD76_02420"
     CDS             5020..5418
                     /locus_tag="PLD76_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26892.1"
                     /translation="MKKFILLLTIFILSSFVAFSAKLPSKGYSGASFSDGGSFMDEPT
                     LSVGNRIGATNSVLGDDACMDACLDEFWDCYNGCGGDTSCPDDCIVAYTACLDACETY
                     SLPIGSGFFFLLLLSGGYAGIKLRRKKKEA"
BASE COUNT         1912 a         1128 c          913 g         1744 t
ORIGIN      
        1 cattaacata gaaaaaatat gatctgcttc agctgcattt tctatggtta cttgtcgcaa
       61 tgttctattt tctgggtcca ttgttgtttg tttcaattgt tgagcattca tctctcccaa
      121 acctttgtag cgttgtatat ggataccacc ttcactcccc ttgccatatt tttctacaaa
      181 agccaaacgt tgcttttcat cccaacagta ctcttcaatc tttccttttc tacacaaata
      241 taagggaggt gtagcaatat acacatatcc attttctatc aaatctttca tatgtcggaa
      301 aaagaacgtt aaaattagcg tagcaatatg actaccatca acatcagcat cggtcatgat
      361 aaccacttta tgataacgta attttgataa atttagtgct ttgctatctt cttctgtccc
      421 aatggtaact cccaaagcag tataaatatt acgaatttct tcgctctcaa acaccttgtg
      481 gggcatggct ttttctacgt ttaatatttt tccacgcaaa ggcaaaatag cctgtgtagc
      541 acgatctcta ccttgtttgg ctgtaccacc tgccgaatct ccctctacta aaaacaattc
      601 gcattttgcg ggatcctttt cggcacaatc ggctaattta ccaggcaatc ctgctcccga
      661 taatggcgat tttcgttgta ctaaatcgcg agctttacga gctgcatgac gggctgtggc
      721 ggctaaaata actttttcga ctatggtgcg ggcttctttt ggatgttcct ctaaataatt
      781 tcccaatgct tcgcttacag ccatatccac cgatcccatc acctcattat taccaagctt
      841 ggttttcgtc tgaccctcaa actgtggctc cgctacctta attgagataa ccgccgttaa
      901 gccctctcta aagtcatttc cgtctatttc tattttattt ttttcaagct tttcgagcaa
      961 tttattctct tctgcatatt tctttaatgt acgggtcaat ccacgtctaa aacctgccaa
     1021 atgagttccc ccctcaatag tattgatatt atttacatat gaatggatat tttcattgta
     1081 agaagtattg tatagcatag ctatttctac tggagttgca tatttatccg ttgatatatg
     1141 aatgacttca tcaattaact tctcacgagc ttgatctata aatctaacaa attctgccaa
     1201 tccttctgtt gaataaaatt cttccgattt gtacgaccca tcttcaaatc tctcacgttt
     1261 atctgtcaaa atcaaacgga tacctgcatt taaaaatgct aactcacgta aacgaccagc
     1321 taaaatatca tatttatatt cagtagtcat aaaaatagat ccatctggct taaaggttac
     1381 agtagttcct ttctgagtag acacgccaat ttctctgaca gaaaattggg gtttaccaca
     1441 cgagtactcc tgctcataaa ccttgccccc tttatgtacc tctaccctca tataatcaga
     1501 caaggcattt acacaagaaa cacctactcc atgcaacccc ccagatactt tatacgaatc
     1561 tttatcaaac tttccaccag catgcaatac tgtcattaca acctcaagag cagacttacc
     1621 ttctttttca tgcatatcaa caggaatacc acgcccatca tcttttacag taatcgaatc
     1681 atcttcgtta ataaatacat caatatgttt acaatagcca gcaagtgcct cgtcaattga
     1741 attatcaacc acctcatata caaggtgatg caaccccttc gtcccaatat caccaatata
     1801 catagccgga cgcatacgta ctgcctccaa cccttctaat acagtaatac tactggctcc
     1861 atacccacca ttttcttttt tttcttcttc gctcataatt tttaaattaa gtaaaaaata
     1921 aacagacaaa tttacataaa cttatcaaga aagcgaaatt ttacagacac aaaatgactc
     1981 taaaatggta atttagattt tccctttttt attctcttat tcatcaatca aataaaaaaa
     2041 tccttgtata tttgcacttt cttatgtctt tgccttataa ccaacatagt attatttagt
     2101 ttatatcatt agcttcaaca tttacttatg aaaaaaaaga ttgctatcat tgctggagga
     2161 aactcttccg aagttatcgt ttctcttaaa agtgctcaag gtatttactc ttttattgat
     2221 aaaaaaacct acgaagtata tattgttacc ttaattgaga aaaaatggtc tgtcaaatta
     2281 gaagatgaaa cagaagtcga catagacaaa aacgactttt cttttatatt aaatggagaa
     2341 aaaatacttt ttgattttgc ctatatcacc atacacggaa cccctggtga aaatgggatt
     2401 ttacaaggat attttgattt aattggatta ccctactcct gctgtggagt attggctgct
     2461 gctattacat tcaataaatt tacgtgcaat caatacctaa aaggatttgg catacctgta
     2521 gccccttcta ttttgctacg taaaaacgaa cacatacaaa acactgtggt tgtcgaaaaa
     2581 atcggcttac cttgtttcat caaatcaaat gtaggtggca gtagctttgg tgtaaccaaa
     2641 gtaaaagaag aaaagcaaat tcaacctgct atcgaaaaag cttttgcaga aggcaatgag
     2701 gttattattg aaggattctt acaaggaaca gaggtaacct gcggaatgta caaaacacat
     2761 gaaaaaacac aggtatttcc tatcacagag gtcgttagtg caaacgaatt tttcgatttt
     2821 gatgccaaat acaacggtca agtaacagaa attactccag cccgtatccc tcaagaagtt
     2881 acaaaccata tacaacaatt aacaaatcag ctatacgata tacttggatg caaaggaatc
     2941 attcgagcag attttattat caacaaacaa aatgtccctt atttactcga agtcaacact
     3001 acacctggaa tgacccctac aagctttata cctcaacaag ttaaagcagc tggtatggct
     3061 atttcatcgg taatggaaga cattattgaa aacaaattaa atcacctata atgtatattt
     3121 aacagtcatt cggcatgaaa agcagagaag aaattatcga aatagtacaa aacgaactaa
     3181 ctcttaccaa ttggctatca gaacctatag acttatataa acccatagcc tatacgatgt
     3241 ctatgggagg aaaacatata cgctctgcaa tcacattgat ggcttgcaac ctatacagcg
     3301 acaatataca tctgcctctt caacctgcca ttggaattga agtttttcat aattttacgc
     3361 ttttgcacga cgacattatg gataaagcag aaacccgtag aggtaagcca accgtacatg
     3421 taaaatggaa cgataacacc gctatcttgt cgggcgatgc catgctcatt aaagcctatc
     3481 aactactggc aaaatgccca ccagaaaacc tttctgaagt actttctgta ttttctcaaa
     3541 cagctattga agtttgcgaa ggacaacagt acgatatgaa tttcgaacat caagatacgg
     3601 tttcagaaga agaatacatt gaaatgattc gtttaaaaac agccgtatta ttggctgcaa
     3661 gcttaaaaat tggtgctatt atcggtggtg caactccact acaagcgaat gctttatacg
     3721 attttggtat tcatattggc atcgcctttc aactacaaga cgacttactt gatgtatatg
     3781 gagatcctac aattttcgga aaaaaaatcg gcggagatat tatctgcaat aaaaaaactt
     3841 ttttgttgat tcgtgcactg aaaaatgcaa acaatactca acgtaataaa atccaacact
     3901 ggatgacaca acatcctgat gacccaaccg aaaaaattgt acaaatgacc tcaatattca
     3961 acgaaacggg tgtaaaacaa gcctgccaac aacgaatgaa agagcattat aacaaagccc
     4021 tacttgcttt gaataaagca tgcgaagtag aagaaaaaat agtacattta caaaaattag
     4081 catcagaact aatgcaaagg gaaaaataat ggaacacgaa tttggatttt acaaaaatat
     4141 aacgtacatt tgcatttatc atattttttg ttaaccttat gccctacaga agacttccaa
     4201 atacagatgc tgcacgtttg agagcactac gggctgttgc ttgctacaaa aattcgccta
     4261 tcgacaccga actgcctttc gatagacgaa tactacaaga aatttgtagc tttttgcctc
     4321 aatttgaaaa tgctatgttt gaatacaaac aagccataaa tagcgaagga aacaaaaaca
     4381 acaaatacca acaatacata aaaaatgtga gattatatat ttcccatttt attcaagtcc
     4441 tcaatttagc gtgtattcgt aacgaaataa aacctgaaaa aaaattattg tataagctat
     4501 tgcccgacaa ttatgccgtg cctgatttaa gcacagaaac tttgcttata gaatggggga
     4561 aaaatattat acaaggagaa aacgaacgaa cacgtgctgg aggttctcct atttacaatc
     4621 cgaccatcgc aaaagtacaa gtacactatg atatttttaa agaagcatat cactcaaaaa
     4681 aagtagcaca gaaaaatgta gggagatatg cagaaaatat gcagaaaatg agaatgcgag
     4741 ctgacgaaat catccttgat gcttggaacc aaattgaaca agcttttgtt cattatggta
     4801 tggatgagat gcaacaaaaa gcaaaagaat ttggaattgt ctattactta agaagaaaag
     4861 agagacaagc aatgacaaaa aaatcgcact aaaaagaaaa cttttgacgt gtttattttt
     4921 ttttgtggaa tatataaatt aaatttgcac cgtattttgt gagaattact cgcggtaatg
     4981 gcttttttat gagcttttta tttactaata cttactcgga tgaagaaatt tatactttta
     5041 ctaaccatat ttattctaag cagttttgta gcatttagtg ccaaattgcc atcaaaagga
     5101 tactctgggg catccttttc ggatggagga agttttatgg atgagccaac cttaagtgta
     5161 ggaaacagaa ttggtgctac taattcagta ttaggagacg atgcatgtat ggatgcttgt
     5221 ttagatgaat tctgggattg ttataatgga tgtgggggag acactagctg cccagatgat
     5281 tgtattgttg cttatactgc ttgcttggat gcttgcgaaa catactctct tcccattggg
     5341 tcaggattct tctttctgtt acttcttagc ggaggatatg ctggcatcaa attaagaaga
     5401 aaaaagaaag aagcataagc ttacatagta attaaactaa tacatattga atagagttgg
     5461 cccaagctga ctctattttt tttgcataaa gttatttaga gttgacagtg gttagtgatt
     5521 agcgtttagt tgaaagtata aagttattaa gtaaacccaa cagcaacaac acccaaactt
     5581 tctaagtttc caatgttaat taatttcagg gcgttgccct gaaacccact cccttttttg
     5641 ttttgataca aaaaacgaag gaaaaaaaat caagactgcg attattcagt ggaaagt
//
LOCUS       DAOSMY010000063         5555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14768_length_5555_cov_4.231455, whole genome shotgun sequence.
ACCESSION   DAOSMY010000063 DAOSMY010000000
VERSION     DAOSMY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14768_length_5555_cov_4.231455"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..2713
                     /locus_tag="PLD76_02425"
     CDS             227..2713
                     /locus_tag="PLD76_02425"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU26893.1"
                     /translation="MRKFTTTHQLRFGLIVLSLFSFLASHAITIYFKDTNNSWGGVNI
                     HIWNAPIETDNSSGWPGKAMVWKATNGSDTWYKIELQSARANMKFNKISNTNTQTSDF
                     NNVSSDGWFHSGSSGIQPISDLPPELNFQLPGAVTMSSDALDGSGVKTATTNAGGGIF
                     TLVKSYTKNLGIDIYYGTSKHTHIATTQIIGHSSSMVGKLAIFTWNANTNVLSIAPAI
                     TITSNLPSPIDAGTNVTLTASAINEPSNYVWEYSINNGGTWLPYTGTKTGTKDRSITV
                     TPSTTTIYRATMESDNETLELLVKKVCAHETRTLFIDDFGTLVNQTDRTTSPYVPATN
                     YTYKPSGKEIRDGHYAVVTTPRFAGCGNQTGDNVAGCNVAGDYWYVDLKDRSNRTTTP
                     FGGMLFVNCRNKDANVAKNVVYARQTINLPPNVELTFSAWFANAQNPTRGTSVDINMR
                     FQILNASNTVVSGSIIDVPAIAAGTGWFYGETTINTGSGGVFTVQILNNGASGVGNDV
                     LIDDIELKVCVPQVSLNVDGQTSVTVTGLHATSTLTVPESVFSLFSSPYYLWVEYNPT
                     TQTWEEVTAFSGVNKPTNNTSFTSYTPSTKQFKVYVASDRQSALDGFNLGTGLYIVTN
                     PVSLDLCDIITQPSANDDVAYTNENTAITIDVTKNDSFGAPNAPKTGTVVITTHPDAS
                     KGTATVNNNGTPTNPTDDKIVFTPKPGYFGDVTMEYEITNYKGDKDRATVTITVSGMR
                     IPDGFSPNGDAINDTFVIQHSSNLTINLIVFNRWGNKVFEQANYANDWEGTGINNQAL
                     PEGTYYRIVEAINNTTGQTQKLSGVITLKR"
     gene            2737..3660
                     /locus_tag="PLD76_02430"
     CDS             2737..3660
                     /locus_tag="PLD76_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU26894.1"
                     /translation="MKKILFIFFACIAGYVVAFAQQAPMYSQYMFNMLNVNPAYAGNR
                     AVQNVTLLHRHQWVGFEGNPRTSSLSWDMRAEDSNNGFGAQIYNDQLGVENTTGLQGF
                     YSYKIPLKNASFALGLSAGLLNFSAAYTEVTTIDPNDPAFAENANTFLPTVGVGLLYA
                     SEKWYLGLSVPALLRTKDFLAEEEALVNKFGATNHYFITGGYVVEVNPNLALKPSLLF
                     KAVSGAPLQTDININAWWNQKWGAGASFRTGDAVVAMLEYQITNTIRLGYAFDYSISQ
                     LNAFNQGTHEIMLRYEFDTSKAIRVLSPRYY"
     gene            3708..>5555
                     /locus_tag="PLD76_02435"
     CDS             3708..>5555
                     /locus_tag="PLD76_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26895.1"
                     /translation="MMKNISYKIRLTLACVLLSATSVQLIAQTNNDILKQADRHYQYA
                     NYARAVQLYEQVVAQTKLLVTQEATPEQTEKLQQEKIDLFTKIADSYWQMRQYNKAKV
                     ALDEIKNISLVKLSNKEKNRLAQLYALTGDYKKAADIYDQIPGAKNKAELYRQDDFFG
                     KLKADSADWTIQLLNINSAYNDFSPVIVHQNILFSSNKPQGKEKRAFGWDGNDYTSLW
                     LFPIKDLKNIQEKPTQKNDKKTTTLQTKKIAGIYTPGDVRPMEQRSSLKNEMPLLPTT
                     QSGKGSRVSGTEKFQFNIATASVDSLGNIFLSVNNQKVKNTQTSSISIMQGSWANQAI
                     SQVKALPLDGFFATMHPTVNKQSTLLIFTAKIAESDSYDLYMSKRNDANQAWSVPVKL
                     AENINSTGNEVFPYIAPDGYLYFSSDGHPGLGGLDLFKIDLQQAINKTGTIESLGYPV
                     NSAADDFGWAQDTTKRAGFFSSNRYANTDNIFSYAYLPIPKSMFVSGLVKEKKSLDLL
                     PGATVFLVDKEKQIVYVDKADKDGRFTFEIKNHGDFTLKGLEENYFEDCIDINVPAPA
                     YKQNQYVAKDILLELQVPKKWVLENLLYDLDKWNIRKDAQAPLDSVVAIM"
BASE COUNT         1833 a         1076 c         1068 g         1578 t
ORIGIN      
        1 gtacagaaat ttagtttatt tacgaagtga aaccgaaaaa taccatgtaa aaccgaagta
       61 tatgctacaa ccgataagtc ttaactaagt tttcttgtct attttatttt ttttgtgttt
      121 atttgcaagt tattaaataa tgtgccgttt tttgcataga aaacacaaaa ctattacaaa
      181 aataaaaaaa tatctaaacc ataaccacct actataccag aatactatga gaaagtttac
      241 aaccacacat caactacgtt ttggattaat cgtattgagt ttgttttctt ttttggcttc
      301 acacgccatc actatttatt ttaaagacac caataattct tggggaggag taaatattca
      361 tatctggaat gctcccattg agacagacaa ttcatcagga tggcctggta aagccatggt
      421 atggaaagcc actaatggtt cagatacctg gtataaaatc gagttgcaaa gtgcgagggc
      481 taatatgaaa ttcaataaaa tatcaaatac gaacactcaa acttcggatt ttaacaatgt
      541 atcgtctgac gggtggtttc attcagggtc gagtggtatc caaccgatat cggatcttcc
      601 tcctgaatta aactttcaat tacctggtgc agttaccatg tcttcagacg cattagatgg
      661 ttcgggtgta aaaacagcca caaccaatgc aggaggagga atttttacat tagtgaaatc
      721 atatacaaaa aatttaggga ttgatatata ttatggtact agtaagcata cccatatagc
      781 aaccactcaa attataggac actcttcatc catggtaggg aaactagcta tttttacgtg
      841 gaatgccaac accaatgtat tatcaatagc accagctata acaataacat ccaatctacc
      901 aagtccgata gatgctggca caaatgtaac gctaacagcg agtgcgatca acgagccttc
      961 taattatgta tgggaatatt caattaataa cggcggaaca tggcttccat atactggtac
     1021 aaaaacagga acaaaagaca gatcgattac agttactcct tctactacta ctatttaccg
     1081 tgctacaatg gaaagtgata atgaaacact cgaattatta gttaaaaaag tttgtgccca
     1141 cgaaacacgt acactattta tagatgattt tggcacatta gttaatcaaa cagacagaac
     1201 gacaagccca tatgttccag ctactaatta cacatacaaa cctagtggaa aagaaattag
     1261 agatggtcac tatgctgttg ttactactcc tcgctttgct gggtgtggaa atcaaactgg
     1321 agacaatgtt gctggatgta acgttgctgg cgattattgg tatgtagact taaaagatag
     1381 atcgaataga acaactacgc catttggagg aatgctattt gtaaattgca gaaacaagga
     1441 tgcaaatgtt gctaaaaatg tggtatatgc acgccaaacg attaatttac ctcccaatgt
     1501 tgaacttact ttttcagcat ggtttgctaa tgcacaaaac ccaacgagag gaacatctgt
     1561 tgatataaat atgcgttttc aaattttaaa tgcttctaat actgtagtca gtggttcaat
     1621 aattgacgtt cctgccattg ccgcaggtac aggatggttt tatggcgaaa cgaccattaa
     1681 tacaggaagt ggtggagtat ttaccgtcca aatattaaat aatggagcat ctggcgttgg
     1741 taacgatgta ctgattgatg atattgaatt aaaagtatgt gttcctcaag tatcactgaa
     1801 tgtggatgga caaacgagcg ttaccgttac aggattacat gccacatcaa cgttaaccgt
     1861 tccagagagt gttttttctt tgttttcttc cccttattat ctatgggtag aatacaatcc
     1921 aactactcaa acatgggagg aagtaacagc attttctgga gtaaataaac caacaaataa
     1981 tacctcgttt acatcgtata caccttcgac caaacaattt aaagtgtatg ttgcaagtga
     2041 tagacaatca gctttggatg gatttaattt aggtacaggt ttgtatattg ttacgaatcc
     2101 agttagctta gatttgtgtg acattattac ccagccctct gcaaatgatg atgtagctta
     2161 tacgaatgaa aatacggcta ttaccattga tgttactaaa aatgatagct tcggagctcc
     2221 taatgctcca aaaacaggaa ctgttgttat tacaactcac cctgatgcaa gcaaaggaac
     2281 tgcaacagtt aacaataacg gaacacctac gaaccctact gatgataaaa ttgtatttac
     2341 cccaaaacca ggttattttg gcgacgtaac aatggagtac gaaattacga attacaaggg
     2401 agataaggat agggcgactg taacgattac tgtctctggc atgcgtatcc ccgatggttt
     2461 ttcacccaat ggcgatgcta tcaacgatac gtttgtgata caacatagct ccaacttaac
     2521 catcaacctt atcgtattta accgatgggg taataaagtg tttgagcaag ctaactatgc
     2581 caacgattgg gagggtaccg gtatcaataa ccaagcacta cccgaaggaa cttactaccg
     2641 tattgtagaa gctataaaca atactactgg acaaacacaa aaactatctg gtgttattac
     2701 tcttaaaaga taagaatcga ctgaaattta ctaattatga aaaaaatact attcatcttc
     2761 tttgcttgta tcgctggtta tgttgtggct tttgctcagc aagctcccat gtactcgcag
     2821 tatatgttta atatgctcaa cgtgaaccct gcctatgccg gcaacagggc agtgcaaaac
     2881 gtaacgcttt tacacaggca ccaatgggtg ggatttgaag ggaatcctcg cacatcatcc
     2941 ctttcgtggg acatgagggc tgaagattct aataatgggt tcggagctca aatttataac
     3001 gaccaactag gtgtggaaaa caccaccggg ttgcagggat tttattccta caaaatacct
     3061 ttaaaaaacg cttcttttgc ccttgggtta agtgctggat tgcttaattt ctctgccgct
     3121 tacacagagg taactaccat agacccgaat gaccctgctt tcgctgaaaa tgcaaataca
     3181 ttccttccta ccgtaggtgt ggggctattg tatgcttccg aaaaatggta tctaggactc
     3241 tctgttcctg ccctgctcag aacaaaagat ttcttggctg aagaagaagc tctggtaaat
     3301 aagtttgggg ctactaatca ctattttatt acgggggggt atgttgttga ggtaaatcca
     3361 aatttagctc tcaaaccatc gctattattc aaagctgttt cgggggctcc tttgcaaaca
     3421 gacattaaca tcaatgcttg gtggaatcaa aaatgggggg ctggagctag tttccgtact
     3481 ggggatgccg tggtagcaat gctcgaatac caaataacaa acaccattcg tttggggtat
     3541 gctttcgatt actctatttc ccaactaaat gcttttaacc aaggtacgca cgagattatg
     3601 ctccgttacg aattcgatac gagcaaagcc ataagggtat tatcgcctcg gtattactaa
     3661 cagggtgctt acttacattg tataacgtct ttaattattt actaatcatg atgaaaaata
     3721 tatcctacaa aataagacta acgctagcct gtgtattgct ttctgctact agtgtacagc
     3781 ttatagctca aacaaacaac gatatcctca aacaagctga caggcattac caatatgcta
     3841 attatgctag agcggtgcaa ctatacgaac aggtggtggc acaaacaaag ctgctggtaa
     3901 cccaagaggc tacgcccgaa caaacagaaa aattgcaaca agaaaaaatt gacctgttta
     3961 ccaaaattgc tgatagctac tggcaaatga gacaatacaa caaggctaaa gttgctctcg
     4021 atgaaataaa aaatataagc cttgtgaaat tatcgaacaa agaaaaaaac aggctcgctc
     4081 aactctatgc tcttacaggt gattacaaaa aagctgccga tatttacgac caaattccag
     4141 gggcgaaaaa caaagctgaa ctctatagac aagatgattt ctttggcaaa ctaaaagccg
     4201 actctgccga ttggactatc cagctcctta atattaacag tgcatacaac gatttctccc
     4261 ctgtcattgt acatcaaaac atccttttct cttctaacaa accccaaggt aaagaaaaaa
     4321 gggcattcgg atgggatggt aatgattaca cctctttatg gttattccct ataaaagacc
     4381 taaaaaatat acaagaaaaa cccactcaaa aaaatgataa aaagacaaca acgctacaaa
     4441 caaaaaaaat agctggcata tatacccctg gtgatgtgcg tcctatggag caacgcagtt
     4501 ccctcaaaaa tgaaatgcct ttgcttccta ctactcaatc ggggaaaggt tcgcgtgtga
     4561 gtggtaccga aaaattccaa ttcaatatag cgactgcaag tgtagattct ttaggaaata
     4621 ttttcttatc tgttaacaac caaaaagtaa aaaatacaca aaccagttct atttccatta
     4681 tgcaaggttc gtgggctaac caggctatat cgcaggtaaa agctctgcct ttggatgggt
     4741 tttttgctac catgcaccct accgttaaca aacaaagtac cctccttatt tttactgcta
     4801 aaatagcaga atcagactca tacgacttgt atatgagcaa gcgtaatgac gctaatcaag
     4861 catggagtgt gcctgtaaaa ttggctgaaa acatcaactc tacgggaaat gaggtgtttc
     4921 cttatatagc tcctgacgga tacttgtatt ttagctcaga cgggcatcct ggattagggg
     4981 gattggattt atttaaaata gacttacaac aagctatcaa taaaaccggt actattgaat
     5041 ccctcggata tcctgttaac tctgctgctg atgattttgg gtgggctcaa gataccacta
     5101 aaagggctgg gttcttctcc tctaataggt atgctaatac agataatatt ttttcgtacg
     5161 cctacttacc tattcctaaa agtatgtttg tgtcaggatt agtgaaagag aaaaaatccc
     5221 tcgatttatt gcctggggct actgtatttt tggtggacaa agaaaaacag attgtgtacg
     5281 tggacaaagc ggacaaggat ggtcgtttca cgtttgagat aaaaaatcat ggcgatttta
     5341 cgctaaaggg attagaagaa aactattttg aagattgtat cgacattaac gtgccagctc
     5401 ctgcttacaa acaaaatcaa tatgtggcaa aagatatttt gctcgagttg caagtaccta
     5461 aaaaatgggt gttagaaaat ctattgtacg acctcgataa atggaacata cgcaaagatg
     5521 cacaagctcc tttggacagt gtggtggcga ttatg
//
LOCUS       DAOSMY010000064         5529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14831_length_5529_cov_3.625685, whole genome shotgun sequence.
ACCESSION   DAOSMY010000064 DAOSMY010000000
VERSION     DAOSMY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14831_length_5529_cov_3.625685"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2271
                     /locus_tag="PLD76_02440"
     CDS             <1..2271
                     /locus_tag="PLD76_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOU26896.1"
                     /translation="RYIEARLSKFALEVVFNPKTTEWKLSYDGRNREPVTLPVKFPLL
                     LAQGIEGIAVGLSSKILPHNFNELIDASIAHLKGEDFEIFPDFIGGGYIDVSRYNQGE
                     RGGSVKVRAKISKLDSKTLVINELPFGETTSSLIDSIVKASEKGKIHIKRVDDNTSAN
                     VEILVHLAPGKSSDKTIDALYAFTNCEVSISPNCCVIYEDKPHFLTVNTVLKHSTDHT
                     LNLLKEELLLQKAELQESIFFSSLEKIFIEQRIYKDKEFEESKNLAEAVAHIDKRLVP
                     FKKLFIREVETEDIERLMEIKMGRILKFNSDKADQHIANLNEQLEQVKHHLAHIVEYT
                     ISWFVGLKEKYGHLYPRKTEIRNFDTIEVTKVVEANQKLYFNREEGFIGTGLKKDEFV
                     CNCSDIDDIILIYKDGKYKVTKVAEKLYVGKNVLYINVFKRNDNRTIYNVVYRNGKDG
                     VSYIKRFAITGVTRDKEYDITQGEAGTKIIYFSANPNGEAEVLKVTLKPKPRLKSLVF
                     ERDFSDIAIKGRMSMGNIFTKNDIHKIVLKQKGGSTLGGREVWFDKDVMRLNYDGRGV
                     SLGEFDSDDSILVLSKDGCFYTTNFELSNHFEDNVLLLEKFDEDKVWSAVLYDAEQQF
                     YYVKRFTLEQTNKKTSFIGDNPESRLLLMSTQVYPRLQVLFGGKDAEREPQEIDVEEF
                     IGVKSFKAKGKRLTTFEIKDIVELEPTRFPEVADEEPENTDEIEENDGNEKVDNEEVI
                     DKKKIIEDITGQMSLF"
     gene            2285..2701
                     /gene="tsaE"
                     /locus_tag="PLD76_02445"
     CDS             2285..2701
                     /gene="tsaE"
                     /locus_tag="PLD76_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU26897.1"
                     /translation="MPQLQIPSLQELPVVATTFIRLLPKDTRIIAFEAGMGVGKTTFI
                     KAICQVLGVQDVVNSPTFSIVNEYACSNGESVYHFDFYRLQSPQEALDFGVYDYFESG
                     NWCFLEWAENIAPLLPTETLFVTLTENPDGSRDIRF"
     gene            complement(3022..>5529)
                     /gene="polA"
                     /locus_tag="PLD76_02450"
     CDS             complement(3022..>5529)
                     /gene="polA"
                     /locus_tag="PLD76_02450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU26898.1"
                     /translation="IKAYGIPIIEVPGFEADDVIGTLAKKAEKAGFITYMVTPDKDYG
                     QLVSERIFMYRPKHTGGYETLGIEEIKEKYQLASHEQVIDLLGLMGDSSDNIPGCPGV
                     GEKTAVKLLQEFGSIDNLLAHTEQLKGALKTKVEENKDLIAFSKFLATIRTDVPVELH
                     ESELILSEPDENQLRTLYEELEFRTFIAKMQASQASKPETSVSKKAPQQGNLFDMFEE
                     NDAEEERTAMGSTSTMDTIDTIKPTYILLNNAKDIDAFVANLLSQKTVCFDTETTGID
                     INESKLVGMSFCWKKNTAYYIPFTSFDETALFLEKIQPLFNNEHTLLIGQNLKFDLLM
                     LQNYGMSVRAKLFDTMVAHYVLQPELRHGMDYLAEIYLKYRTIHYEDVVGVKGKNQQN
                     IQDIDPAIVCNYAAEDADITFQLKEILEKELHANRLEKLFFDIEMPLVKTLCTIERAG
                     VRIDTQALSQSSLVLTEQMQEIEHNVQQHVGFEFNLSSAKQVGEVLFDRLKITDKAKK
                     TKTGQYATSEDILEELRGKHPIVPLILEYRGLKKLLSTYIDALPLLVNKHSGKIHTSY
                     NQTITATGRLSSSNPNLQNIPIRDAQGKEIRKAFIPDEGCLFLSADYSQIELRIMAHL
                     SQDENMVDAFLSGHDIHAATAAKIYKIPIEEVSSDMRRKAKTANFGIIYGISVFGLSD
                     RLSISRSEAKELIDGYFDTYPGVKSYMEASIAKAKEQGYVETLLGRKRFLPDINSSNS
                     FVRGFAERNAINAPIQGSAADIIKIAMIRITQRFEQENIQSQMTMQVHDELNFSVLKS
                     ELEIVKRIVIEEMEHAISLRVPLVADCGVGDNWLEAH"
BASE COUNT         1635 a          932 c         1128 g         1834 t
ORIGIN      
        1 cgttatatcg aagctcgttt gagtaaattt gccctcgaag tggtgtttaa cccaaaaact
       61 accgagtgga aattgtcgta tgatggaaga aacagagagc ctgtaacttt acccgttaaa
      121 tttcctttgt tattagctca aggaatagag ggtattgcag taggattgtc ttcaaaaata
      181 ttgccacaca attttaatga gttaattgac gcttctattg cccatctcaa aggcgaggat
      241 tttgaaattt ttcctgattt tatcggggga ggatatattg atgtgagtcg gtacaaccaa
      301 ggcgagcgtg gcggaagtgt aaaagtgcgt gctaaaatta gcaaattaga tagtaaaaca
      361 ctggtaataa atgagctacc atttggcgaa acgactagtt cgttgattga ctcgattgta
      421 aaagcatcgg aaaaaggcaa aattcatatc aaaagggtgg acgataatac ttctgctaat
      481 gtggaaattt tagtgcattt ggctccagga aaatcatcgg acaaaaccat tgatgccttg
      541 tacgcattca cgaattgtga agtgagtata tcgcccaatt gttgtgttat atacgaagat
      601 aaaccacatt ttttaaccgt taatactgta ctaaagcatt ctacagatca tactctcaac
      661 ttgttgaaag aggagttgtt gctacaaaaa gctgaactgc aagaaagtat ctttttctct
      721 tcgctcgaaa aaatatttat cgaacaacgt atttataaag ataaagagtt tgaagaaagt
      781 aaaaacctgg ctgaggcagt agctcatatt gataaacgat tagtcccttt taaaaagcta
      841 tttatccgcg aagtagaaac agaagatatt gagcgtttga tggaaataaa aatggggcgt
      901 atcctcaaat ttaattctga taaggctgac cagcatattg ctaatttaaa tgagcaattg
      961 gagcaagtaa aacaccattt ggctcatatt gtggaataca caatcagttg gtttgttgga
     1021 ttaaaagaga aatatggtca tttatatcct cgtaaaactg aaattcgtaa tttcgatacc
     1081 atagaggtaa ccaaagtggt tgaagccaat cagaaattgt attttaatcg cgaagaaggt
     1141 tttatcggaa caggcttaaa aaaggatgag tttgtgtgta attgttcgga tattgatgat
     1201 attatcttga tttacaaaga tggaaaatac aaggtaacta aagtagctga aaaattgtat
     1261 gtgggtaaaa atgtgctgta tatcaatgtg tttaaacgga atgacaaccg tactatttac
     1321 aatgtggtat atcgcaatgg aaaagatggt gtgagttaca tcaaacgttt tgctattacg
     1381 ggtgtaacac gcgacaaaga gtacgatatt acccaaggag aggctggtac aaaaatcatc
     1441 tattttagtg ccaatccgaa tggagaagcg gaggtattaa aagttacctt aaagccaaaa
     1501 cctaggttga aatcgttggt ttttgaacgc gactttagtg atattgccat caaaggacgt
     1561 atgtcgatgg gaaatatttt taccaaaaac gatattcata agattgtatt aaagcaaaaa
     1621 ggcggttcta ccttaggggg tcgcgaagtg tggtttgata aagatgtgat gcgattaaat
     1681 tatgatggaa gaggcgtatc gcttggcgaa tttgatagtg atgattcgat tttggtatta
     1741 tccaaggacg gttgttttta tactacaaat tttgaattga gtaatcattt tgaagataat
     1801 gtcttgttgc tcgaaaaatt tgatgaagat aaggtgtggt cagctgtact gtacgatgct
     1861 gagcaacagt tttattatgt aaaacgtttt actttagaac aaaccaacaa aaaaacatca
     1921 tttattggag ataatcccga atcacgtttg ttgctgatgt ctacgcaggt atatccacgt
     1981 ttgcaagtat tgtttggtgg taaagatgcc gagcgtgaac cgcaagaaat tgatgtagaa
     2041 gagttcattg gcgtgaaaag ttttaaagcc aaagggaaac gtttaactac atttgaaatc
     2101 aaagatattg tagagttgga gccaacacgt tttccagaag tagctgatga agagcctgaa
     2161 aatactgatg aaatcgagga aaatgatgga aatgaaaaag tagataacga agaagtaata
     2221 gacaaaaaga agataattga agatattact ggtcagatga gtttgtttta ataaaatctc
     2281 ctttatgcca caacttcaaa taccatcgtt gcaggaactt ccagtagtag ctactacttt
     2341 tatacgctta cttcccaaag atacccgtat catagctttt gaagcaggta tgggggtagg
     2401 taaaactact tttatcaaag ctatttgtca ggtgttgggt gtgcaagatg ttgtcaatag
     2461 tcctacgttt tctatcgtga atgaatacgc ttgtagcaat ggcgaatctg tatatcattt
     2521 tgatttttac cgattacaat ctccccaaga ggcattggat tttggagtgt acgattattt
     2581 tgaaagtggc aattggtgtt tcttggaatg ggcagaaaat attgccccct tattaccgac
     2641 ggaaactctt ttcgtaaccc taacggaaaa tcccgatgga agcagggata ttcggtttta
     2701 ataaatgtat gtattggggt tttttatgtc gctcctatgg agcttacttt gtttgctgtg
     2761 tttttattct attattatat cgcccctctg gggcttagtt tgaaggaatt agttagcgtc
     2821 tatttgattt tccattttct tgtattttta taccttaaac gatttcttat caaatgtgtt
     2881 tcgtgctccg taggagtacc atcataatag ggtaatagac aaatgaatag cgaagatccg
     2941 taggagcgac ataaaacttg cttttattgc tttatgttat tattatatcg cctcgctgga
     3001 gttttggtgg ggagaaaatt attaatgtgc ctctaaccaa ttatctccta caccacaatc
     3061 agccactaaa ggtactcgta gtgaaatagc atgttccatc tcttcaatta cgatacgttt
     3121 tacgatttcc agttcacttt ttaacacgct aaagttcagc tcatcatgca cctgcatggt
     3181 catttgtgat tgtatatttt cttgttcaaa tcgttgtgta atacgtatca tggctatttt
     3241 gattatatcg gcagcagagc cttgaatggg tgcattgata gcatttcgtt cggcaaagcc
     3301 acgtacaaaa ctattggagg aattaatatc aggtaaaaac cgtttacgac ctaaaagtgt
     3361 ttctacgtat ccttgttctt tggctttcgc aatgctcgct tccatgtatg attttactcc
     3421 tgggtaagta tcaaaatacc catctatgag ttctttggct tcgctgcgtg agatgctgag
     3481 tcggtcgctg agtccaaata ccgagatacc gtaaatgata ccaaaatttg ctgttttggc
     3541 tttgcgtcgc atatcactgc tcacttcttc gattggaatt ttgtaaattt tcgccgctgt
     3601 agccgcatgg atgtcgtgtc ctgaaagaaa tgcgtctacc atattttcat cttgactcaa
     3661 atgtgccata atccgcaatt caatttgcga ataatccgca gaaaggaata aacacccctc
     3721 atcggggata aatgcttttc ttatttcttt gccttgagca tctcgaatgg gtatattttg
     3781 caggttcgga ttgctcgaac ttagtcgccc cgtagctgta attgtttgat tgtaagaagt
     3841 gtgtattttt cctgaatgtt tgtttactaa tagaggcaaa gcatcaatat acgtgctaag
     3901 gagttttttt aatcctctgt attcgagtat gagagggaca ataggatgtt tgcctcgtaa
     3961 ttcttctaaa atatcttcag aagtggcata ttgccctgtc tttgttttct tcgctttatc
     4021 tgtaattttc aatcggtcga acaatacttc tccaacttgt ttagccgaac taagattgaa
     4081 ttcaaatccc acgtgttgtt gtacgttgtg ttcaatttct tgcatttgtt cggtaagtac
     4141 caacgaagat tgagacaatg cttgggtatc aatacgtaca cctgctcgtt cgatagtaca
     4201 tagggttttt actaaaggca tttcgatatc gaagaaaagt ttttctaagc gattggcatg
     4261 aagctctttt tcgagtattt cttttagctg aaaagttatg tcggcatctt ctgctgcata
     4321 attacatact atagcagggt caatatcttg tatattttgt tgattttttc ctttaactcc
     4381 tactacatct tcgtagtgaa ttgtgcggta tttgaggtaa atttcggcta agtaatccat
     4441 cccatgtctc aattcgggtt ggagtacata atgagctacc atcgtgtcga atagtttagc
     4501 tcgtacagac atgccgtaat tttgcaacat taataggtca aattttaggt tttgtcctat
     4561 taaaagggta tgttcgttgt taaatagtgg ctgaattttt tctaaaaaaa gtgcggtttc
     4621 atcgaaagaa gtaaaaggta tgtagtaagc agtatttttt ttccaacaaa acgacatgcc
     4681 tactagtttg ctctcattga tatctatgcc tgttgtttct gtatcaaaac aaaccgtttt
     4741 ttgagacaat aaattcgcta caaaggcatc tatatctttc gcattattca gtaaaatata
     4801 ggtaggttta attgtatcga tggtatccat tgttgaagtt gatcccattg ctgtacgttc
     4861 ttcttcagca tcattttcct caaacatgtc aaacaaattg ccttgctgag gagctttttt
     4921 tgaaacactt gtttctggct tactggcttg tgatgcctgc atttttgcaa taaaggtgcg
     4981 aaactcaagt tcttcgtata gtgtacgcaa ttgattttcg tctggttcgc tcagtataag
     5041 ttccgattca tgtaattcaa caggtacgtc ggtacgtatt gtggctaaaa attttgagaa
     5101 agcgattaag tccttgtttt cttccacttt cgtttttaag gcacctttca gctgttcagt
     5161 atgagccaac agattgtcaa tactaccaaa ttcttgtaac agtttgacgg cggttttttc
     5221 gcccacgcca gggcagcccg gtatattatc gctactatcg cccatcaatc cgagcaaatc
     5281 tattacttgt tcgtgagaag ctaattggta tttttctttt atttcttcta ttcctagagt
     5341 ttcataccct cccgtatgtt tgggtcggta cataaaaata cgttcagaca caagttgccc
     5401 ataatctttg tcgggagtta ccatataagt aataaaaccc gctttttctg ctttttttgc
     5461 gagagtacca attacgtcgt ccgcttcaaa accaggcacc tcaataatag gaataccata
     5521 cgctttaat
//
LOCUS       DAOSMY010000065         5500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14934_length_5500_cov_3.581084, whole genome shotgun sequence.
ACCESSION   DAOSMY010000065 DAOSMY010000000
VERSION     DAOSMY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14934_length_5500_cov_3.581084"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2254
                     /locus_tag="PLD76_02455"
     CDS             <1..2254
                     /locus_tag="PLD76_02455"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOU26899.1"
                     /translation="ENPSWKHLHATFLVVKNTLDALQQLAAFHRVVYNIPIVGITGSN
                     GKTIVKEWLHQLLQSDYHLTRSPKSYNSQVGVPLSICLLDETTEIGIFEAGISQPHEM
                     QKLQHIIQPNIGIFTTLGQAHQENFSSMEEKLREKLYLFSSSKVLICKYNHAIEEILR
                     PINPTISYVSWSFSIDDNPTILIQSVIKKHTESSIRFCYQQQEFSYTIPFVDDASIEN
                     SIHCLAFCLWLSVPIYTIIDRMKRLEPIAMRLEKKQGTNNCLLINDSYNSDINSLHIA
                     LDLVDYSAENTYDKRTVILSDIIQSGEEEHILYSKVAKAIQQKNISRFIGIGKHISQY
                     AHLFEGKTYFYHTTKEFLFSNIIDSFHNEAILVKGARSFEFEKIINKLELKMHETVLE
                     VNLNALVNNFNYLRSYLLPSTKTMCMIKANAYGCGAIEVAKTLQYHRCDYFGVAVADE
                     GVELRKAGIHTPIIVMNPEPSSFDLLCEYRLEPEIYSFKLLKAFAEHLNKYGIINYPI
                     HIKIDTGMKRLGFEPTDMNTLIHFIKAQTSCQVQSIFTHLVGADDAIFDDFTQQQLKL
                     FTQCCDVLQSHCNHPIIRHALNSAGVERFPNFQFDMVRLGIGLYGIATSNQHAIQPLV
                     TLKTIVLQVKNIAAHETVGYSRKGSLHRNSRIATIPIGYADGLKRNLGNGNGYVLIDG
                     KKAPFVGNICMDISMVDITDIPHVEEGDTVVVIGNQIHINEIAEKAGTIPYEILTGIS
                     QRVKRIYFQE"
     gene            2384..2965
                     /locus_tag="PLD76_02460"
     CDS             2384..2965
                     /locus_tag="PLD76_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26900.1"
                     /translation="MKKVWMYIVLSTVLFTGCIADKRNLQFVESLVETELSNDALQLR
                     YRAPKGFHLVDQHYTDSIANVQLLQDGFSPVVLSIHVDTLKGASMMLSDIRYLPEDKL
                     EKEIKNYKKTYNQHNTWNKVNRMTYSHHNFDIVEINCENDTYLLTKLFFYEDKHPTFV
                     VDYFIPIVEKETYTPLINSSIASFEKGYTLIFH"
     gene            complement(2970..3647)
                     /gene="mtgA"
                     /locus_tag="PLD76_02465"
     CDS             complement(2970..3647)
                     /gene="mtgA"
                     /locus_tag="PLD76_02465"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOU26901.1"
                     /translation="MKVVRILRNIVLGLFLSSILLVILYRFVPVYITPLMIIRVAEQV
                     ADGKPIRLERGWVPIDEISPKMVRAVIASEDNLFLDHYGFDIEGMKKAFENNKKGEKL
                     KGGSTISQQTAKNVFLTPSRTYVRKALEAYFTVLIEIFWSKERIMEVYLNVIEMGNGI
                     YGIEKASEIYFNKPPKKLTSFQAALVAASLPNPRKYSITHPGPYMRKRQAHILWLMPK
                     LGKIEFE"
     gene            complement(3665..4702)
                     /gene="asnA"
                     /locus_tag="PLD76_02470"
     CDS             complement(3665..4702)
                     /gene="asnA"
                     /locus_tag="PLD76_02470"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOU26902.1"
                     /translation="MGKLIVPQTYTSLLDLQQTELAIKILKDFFQTSFSAELRLRRVT
                     APLFVLQGMGINDDLNGVERAVSFPVKDLGDVKAEVVHSLAKWKRIILADYAIENGYG
                     VYTDMNAIRSDEELDNLHSLYVDQWDWEMVIAKEDRTIAFLQQVVKRIYAVMVRAEYA
                     VYEYFPTIKPSLPRDIFFIHAEELLAMYPDKSPKEREYMVTKEHGAVFIMGIGGQLAN
                     GEKHDGRAPDYDDWTTIGENGLAGLNGDILVWDSVLQLPLELSSMGIRVDKDALIKQL
                     AITHTEERLQFYYHKRLVEGSLPLSIGGGIGQSRLCMFMLRKAHVGEIQASIWPDSMR
                     KECKELGIFLL"
     gene            4924..4995
                     /locus_tag="PLD76_02475"
     tRNA            4924..4995
                     /locus_tag="PLD76_02475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Arg,seq:ccg)
     gene            5078..5293
                     /locus_tag="PLD76_02480"
     CDS             5078..5293
                     /locus_tag="PLD76_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU26903.1"
                     /translation="MLAKKTLITLRQYFKEYKPKEWLFEGESGTDLRYIQSLLGHSSS
                     KTTEIYTHITNKGFDQIKNPLDKLEIY"
BASE COUNT         1934 a         1022 c          885 g         1659 t
ORIGIN      
        1 agaaaatcca tcgtggaaac atctacatgc cacattttta gttgttaaaa atacactaga
       61 tgccttacaa caattagctg cttttcatcg tgtagtatac aatattccaa tcgtaggtat
      121 tactggtagc aatgggaaaa caattgtaaa agaatggctc catcaactct tgcaatcaga
      181 ttaccaccta acacgctctc ctaaaagcta taattcgcaa gtaggagtac ctttatctat
      241 ctgtttactt gatgaaacaa cggaaatagg tatttttgaa gcgggcattt ctcaaccaca
      301 cgaaatgcaa aaactgcaac acattatcca acctaacatt ggtatattta ccactcttgg
      361 acaagctcac caagaaaatt tttcctctat ggaagaaaaa ttacgcgaaa aactatattt
      421 attttcttca tcaaaggtat taatctgtaa atataaccat gctattgaag aaatattacg
      481 cccaattaat ccaactattt catatgtttc ttggtcattt tctatagatg acaacccaac
      541 gatactcatt caatctgtta taaaaaaaca tacagaaagt agtattcgtt tttgctatca
      601 acaacaagaa ttttcgtaca ccataccatt tgtagatgat gcatctattg aaaatagtat
      661 acactgttta gctttctgcc tatggctttc tgttcctata tacactatca tagatagaat
      721 gaaacggtta gaacccattg ctatgcgact tgaaaaaaaa caaggaacaa acaattgttt
      781 actaatcaac gatagctata attcggacat taactccttg catatagcat tagatttggt
      841 cgattattca gcggaaaaca cctacgataa acgcaccgta attctgtcag acatcattca
      901 atcaggcgaa gaagaacaca tattgtatag taaagtagcg aaagcaatac aacaaaaaaa
      961 tatttctcgt tttattggca ttggaaaaca catatcccaa tatgctcatt tatttgaagg
     1021 aaaaacctat ttttatcaca caacaaaaga gtttttattt tccaatataa ttgacagttt
     1081 tcataacgaa gccattcttg taaaaggagc tcgatctttt gaatttgaaa aaatcatcaa
     1141 caaattagaa cttaaaatgc acgaaacggt gctagaagtt aacctcaatg cactggtaaa
     1201 caactttaat tatttacgct cttacctatt acccagtacc aaaacaatgt gtatgataaa
     1261 agccaatgca tatggttgtg gtgcaataga agtggctaaa acactacaat accaccgatg
     1321 tgattatttt ggggtagctg tagcagatga aggtgttgaa ttacgcaaag cgggtattca
     1381 tactcctatt attgtaatga atccagaacc aagctcgttc gatttgctgt gtgaataccg
     1441 tttagaaccc gaaatatata gttttaaatt actgaaagct tttgccgaac accttaataa
     1501 atatggtatt atcaactacc ctattcatat caaaatagat actggcatga aacgattggg
     1561 ttttgagcct accgatatga atacattaat tcattttatc aaagcacaaa catcgtgtca
     1621 ggtacagtct atttttacgc atttggtagg ggctgatgat gctatatttg atgattttac
     1681 gcaacagcaa ctaaaacttt ttacccaatg ttgtgatgta ctacaatccc attgtaacca
     1741 ccccattata cgtcatgcac taaatagtgc tggagtagaa agatttccta actttcagtt
     1801 cgatatggtt cgtttgggaa taggtttgta tggaattgca acatccaacc aacatgctat
     1861 tcagccttta gtaaccctaa aaactatcgt attgcaagta aaaaacatag ctgcacacga
     1921 aacagttggt tatagcagaa aaggtagctt gcaccgaaac tcccgtatcg caaccatccc
     1981 aattggatat gctgatgggc taaaacgtaa cctaggaaat ggaaatggat atgtactcat
     2041 tgacggtaaa aaagctcctt ttgtaggcaa tatttgtatg gatatcagta tggtagatat
     2101 tacagatata ccacatgtag aagagggaga tacggttgtg gtgattggaa atcaaataca
     2161 tatcaatgaa atagcagaaa aagcaggcac tattccttat gaaatattaa caggtatatc
     2221 tcaacgagta aaacgtatat attttcaaga gtaataagat gaaaggattt gctgtgtaag
     2281 cgttgtgcac aaaaagcaca aaagaaataa cagaacaaaa ttgcagagaa tgtagtataa
     2341 aactctgtgt aagaaaaata actagataat aactgaataa cagatgaaaa aagtgtggat
     2401 gtatattgtt ttgagtaccg tgttgtttac aggttgcata gcagataaac gcaacctaca
     2461 atttgtggaa tcattggtag aaacagaatt atctaacgac gcattacagc ttcgctatcg
     2521 tgctccaaag ggttttcatt tagttgacca acattataca gattctattg ccaatgtaca
     2581 attgctacaa gatggttttt ctcccgttgt tttgagtata catgtagaca ctctaaaagg
     2641 agcttctatg atgctttcgg atattcgata tctacccgaa gataaattgg agaaagaaat
     2701 taaaaactac aaaaaaacat acaatcaaca caatacttgg aataaagtaa atcgcatgac
     2761 gtatagccat cataattttg atatagtaga aataaactgc gaaaacgata cctacttgct
     2821 cacaaaatta ttcttttacg aagataagca tccgactttt gttgttgatt attttattcc
     2881 aatcgtagaa aaagaaacat ataccccact tataaattca tccattgcat cgtttgaaaa
     2941 aggatataca ctcatctttc attaatgtgt tattcaaact caatttttcc taacttgggc
     3001 attaaccata gtatatgtgc ttgtctttta cgcatataag gacctggatg tgttatgcta
     3061 tacttacgtg gattaggcaa cgatgctgca accaatgcgg cttgaaaaga agttaatttc
     3121 ttaggcggtt tattaaaata aatttctgag gctttttcta taccgtaaat accatttccc
     3181 atttcaatca cattgagata cacctccata atacgctctt tggaccaaaa aatctcaatc
     3241 aaaaccgtaa aataagcttc caaagcttta cgaacatacg tacgactcgg ggttaaaaaa
     3301 acatttttgg ctgtttgttg tgaaatagtg ctcccccctt ttaatttttc ccccttcttg
     3361 ttattttcaa aggctttttt cattccttcg atatcaaaac cgtaatggtc taaaaaaaga
     3421 ttatcttctg aggcaataac agcacgtacc attttgggtg aaatttcgtc tattggcacc
     3481 cagcctcttt ctaatcgaat gggtttacca tcagccactt gttctgccac cctaataatc
     3541 attaaaggag taatataaac aggcacaaaa cgatacaata taacgagcag gatactggat
     3601 aaaaataatc caagtacgat gttacgaaga atacgcacta ctttcattca aattggtaaa
     3661 tttcttatag caaaaatata cctaactctt tacattcttt acgcatacta tcgggccaaa
     3721 tacttgcttg tatctcacct acgtgagcct tacgtaacat aaacatacac aagcgagact
     3781 gacctatacc accaccaata gacaagggta atgaaccttc tactaagcgt ttgtggtaat
     3841 aaaattgtaa acgttcttct gtatgtgtta tagctaattg tttgatcaac gcatctttat
     3901 ctacgcggat acccatagat gataattcta aaggcagttg caaaacagag tcccacacca
     3961 aaatatcacc atttaatcct gctaacccat tttcgcctat ggtagtccaa tcatcgtagt
     4021 ctggggcacg accatcgtgt ttttcaccat tggctaattg ccccccaata cccatgataa
     4081 acacggcacc atgttctttg gtaaccatgt attcgcgttc tttgggcgat ttatctggat
     4141 acattgccaa caattcttcg gcatggataa agaaaatatc gcgaggtaat gacggtttga
     4201 tagtaggaaa atattcatat acagcatatt cggcacgtac cattacggca tatatacgtt
     4261 ttactacttg ttgtaaaaat gcaatggttc tgtcttcttt tgcaatcacc atttcccaat
     4321 cccattggtc tacatacaaa gaatgtagat tatctagctc ttcatcagag cgaatagcat
     4381 tcatatccgt atatacacca tagccattct caatagcata gtcggctaaa ataatgcgtt
     4441 tccactttgc caaagagtgt acaacctctg cttttacatc acccaaatcc tttacaggaa
     4501 aactaacagc gcgttctacc ccatttaaat cgtcgttaat ccccatacct tgtaatacaa
     4561 acaagggagc agttacacga cgtaaacgta gttcggcaga aaaactagtt tgaaaaaaat
     4621 ctttcagaat tttaatagcc aattctgttt gttgcaaatc caataaggat gtgtaagttt
     4681 gaggcacaat gagttttccc ataaaaaatt gcattttttt aataatggtt gcaaaactac
     4741 agaatattat tgtaaaaaaa gaacatatac ctatttttat tagcaatata acataaaaaa
     4801 cagcgaaaga acgtacaaaa agacatctag tgatcattat ttatttacat acccatcagg
     4861 ataaattact atttgcctct atcattaaaa aaaaagatgt atttttgcac caccttatat
     4921 cacggtccta tagtttaatg gataaaacgt aagattccgg ttcttatgat ggaggttcga
     4981 ttcctcctag gatcacacta aagattgata aaaaaatctc tcccctatct tcccttgtat
     5041 tacacacatt ttacactgaa aattagcctc aattattttg ctggctaaaa aaactttaat
     5101 aactttacgc caatatttta aagaatacaa acccaaagag tggttgtttg aaggagaaag
     5161 tgggacagac ttacgatata tacaaagttt gttgggtcat tcgagtagta aaacaacgga
     5221 gatatataca catattacaa acaagggatt tgatcagata aaaaatcctt tggataaatt
     5281 agaaatatac taaaattaac aaatggatag gtgccacggt atacttttat taggaaaata
     5341 taaactgcat attatcagta gtttaaacaa atacgaatag gtgtataaag tgaatatagt
     5401 taccacgcta tggtacctat acaaaaacgt ggtacataca cagatgttgg caacaagtgt
     5461 aaaagacaga cgagcagaca gaaacgatag tgataaataa
//
LOCUS       DAOSMY010000066         5491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_14967_length_5491_cov_4.154893, whole genome shotgun sequence.
ACCESSION   DAOSMY010000066 DAOSMY010000000
VERSION     DAOSMY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14967_length_5491_cov_4.154893"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..773
                     /gene="pyrE"
                     /locus_tag="PLD76_02485"
     CDS             141..773
                     /gene="pyrE"
                     /locus_tag="PLD76_02485"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU26904.1"
                     /translation="MENLSTLTASKLLKINALKLQPTNPFTWASGWKSPIYCDNRKTL
                     SYPDVRNFIKLQMTRIILEKYPDVQAIAGVATGAIAQGALVAAELNLPFVYIRSSKKD
                     HGLENLVEGDLQTGQKVVVIEDLISTGGSSLKAVDAIRNNGCEVLGMVAIFTYGFPVA
                     QQQFSEAKVELTTLSNYEAVLALALETNYIKESDITTLQEWSKSPSTWNA"
     gene            784..1191
                     /locus_tag="PLD76_02490"
     CDS             784..1191
                     /locus_tag="PLD76_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOU26905.1"
                     /translation="MTQFDSEIKKINATSEIVFNKLTNLENLSQLEGSMPHEKVKILK
                     ITENTCLFEVEMIGEIGIKIANKEPHHLILFESQQSPLAFNLSIYLTEQNEHTELQIQ
                     LKADLPMMVKMMVSEPINKFVNILADTLTRLSY"
     gene            1196..2533
                     /gene="argH"
                     /locus_tag="PLD76_02495"
     CDS             1196..2533
                     /gene="argH"
                     /locus_tag="PLD76_02495"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU26906.1"
                     /translation="MAQKLWEKDTKVNSEIERFTIGKDREMDMYLAKYDVIGSMAHIT
                     MLESIGLLTPKELTTLLLGLKEIYKEIESGHFIIDENVEDIHSQVELVLTDKLGDMGK
                     KIHSGRSRNDQVLLDLKLFSRAKIQEIATETHLLFNTLLTQSEQYKSVLMPGYTHLQI
                     AMPSSFGLWFGAYAESLVDDMQLLLAAFKVCNRNPLGSAAGYGSSFPLNRQKTTDLLG
                     FDSMNYNVVYAQMGRGKMERTVSTALASIANTIAKLAFDSCLFTSQNFGFIKLADEFT
                     TGSSIMPHKKNPDVFELTRAKCNKIQGIPQQINLITNNLPSGYFRDLQIIKELFIPMF
                     DELIECIRMTRYIIEKVEINTHILDDKKYQAIFSVEEVNKQVLSGIPFRDAYKNIGLA
                     IEKGDFHPDTSISHTHEGSIGNLCNEEINSLMNEIISAFNFKSIQEAEAKLLL"
     gene            2545..3312
                     /locus_tag="PLD76_02500"
     CDS             2545..3312
                     /locus_tag="PLD76_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU26907.1"
                     /translation="MLFSSIIFGPIKSRRLGNSLGINLLPTDAKICSFDCIYCECGLN
                     DSTKKGNIPTREAVKNALSLELEKLKKQDITIDTITFAGNGEPTMHPDFKEIIDDTLI
                     IRNQLAPDAVISVLSNAWHITNPKVYGALLKVDNNILKLDSAIKETILQINHPNNTSF
                     EVSELIANLASFQGQCIIQTLFVKGNHKGNLIDNTTEEEINAWLNALKTIQPKQIMIY
                     SIDRETPIKSLEKIPSSTLKAIASQAKKLGFNISVSV"
     gene            3309..4370
                     /locus_tag="PLD76_02505"
     CDS             3309..4370
                     /locus_tag="PLD76_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU26908.1"
                     /translation="MKTAKRILICPLNWGLGHATRCIPIIHKLQQEGNILLIASSGCA
                     GELLQQEFPTLEYIHFPGFSITYSKHHSQTLRIVLSFPKIIYYSFKEHFVLKKLIKIH
                     DINCVISDNRFGLWNKDITTAYITHQLNIKLPKHVKIFEKLTWYIHRYFIEKYNYCII
                     PDNQQSTLSLAGDLVHQRKLPKNAFFCSPLSRFSKSIQNTSTKFQSNILIILSGIEPQ
                     RSILEKLLLQQLTKINQKAILIQGLPNETKYKKNIGNITIISYVLHQELQELINNTDL
                     VICRSGYSSIMDLVAINKQAIIIPTPGQTEQEYLAKYLHEKKFFIAQQQDNIDILKGM
                     EESKSTKIPDMKFYLQLPF"
     gene            complement(4371..5027)
                     /gene="upp"
                     /locus_tag="PLD76_02510"
     CDS             complement(4371..5027)
                     /gene="upp"
                     /locus_tag="PLD76_02510"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOU26909.1"
                     /translation="MRTHNLALQDSLLNTFLEQIRSKEVQKDRMRFRRNMERIGEVMA
                     YEISKSLAYSTKNIATPLGIANVNTVDNDIVLGTIFRAGLPFHQGFLSYFDNADNAFI
                     SAYRKYKDALKFEVFIEYIASPDLTGKTLILVDPMLASGSSMELAYNALLTRGKPKEV
                     HFATIIASQYAVDYLSNHFAEESNISLWSAAIDPELNAHSYIIPGLGDAGDLAFGEKL
                     "
     gene            complement(5024..>5491)
                     /locus_tag="PLD76_02515"
     CDS             complement(5024..>5491)
                     /locus_tag="PLD76_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26910.1"
                     /translation="RVRVFLNRFHFLKFTFPISFEYLESLSIARDYSLGYAKYTGFRA
                     STCTPFLFYNLTKDYISGMRLHPIVLMDVTLQKYLHKEATDLFGIMSYYAEVIKKYNG
                     EFVTLFHNESLSDEDQWYGWREEYKKSVQYVALLELHSIEVAKEKLANQHMGV"
BASE COUNT         2030 a          985 c          848 g         1628 t
ORIGIN      
        1 agatggttct gcttgtgcca tttatggagt gcaaggaaac aatggtgtaa ttcttataaa
       61 tttgaaataa gcattttttt ttccaattct attttttacc tttgtgaaac ttaatcatat
      121 ttactaaatt tagttttaaa atggaaaatc tatcaaccct tacagcatct aagttactta
      181 aaataaacgc attaaaatta caaccaacca atccctttac atgggcatca ggatggaaat
      241 cacctattta ttgtgataat cgtaaaacgt tatcatatcc agatgtacgt aattttatta
      301 aacttcaaat gacacgtatt attctagaaa aatatccaga tgtacaagcc atagcggggg
      361 ttgcaactgg agctattgca caaggagcat tggttgctgc ggaactaaac ctcccttttg
      421 tatatatacg ttcttcaaaa aaagaccatg gactagaaaa tttggtagaa ggtgatttac
      481 aaactggtca aaaagtagta gttatagaag atttaatatc aacaggaggc agtagtttaa
      541 aagcagtgga tgctattcgt aataatggtt gcgaagtgtt aggaatggtc gccattttta
      601 cgtatggttt tccagtagct caacaacaat ttagcgaagc aaaagtggaa ttaacaactt
      661 tatcaaacta tgaagcagtc ctagctcttg ctttagaaac taattatatt aaagaaagtg
      721 atataaccac cctacaagag tggagtaaaa gcccatctac ttggaacgcc taatacaaac
      781 agaatgacac aattcgacag cgaaattaaa aagattaacg ctacctcaga aattgtattt
      841 aataaactta caaatctgga aaaccttagt caacttgagg gctcaatgcc acacgagaaa
      901 gtaaagattc tgaaaattac agaaaataca tgtctatttg aagtagaaat gattggcgaa
      961 attggcatta aaatagccaa caaagagcct catcacctta ttttattcga gtctcaacaa
     1021 tctcctttgg catttaattt atctatatac ctaacagaac aaaacgaaca taccgaatta
     1081 cagattcagc taaaagcaga tctacccatg atggtaaaaa tgatggttag tgaacctatt
     1141 aataaatttg taaacatttt agcagataca ttaactagat tatcctatta aaaatatggc
     1201 acaaaaactt tgggaaaaag acacaaaagt caatagcgaa atcgaacgtt ttactatcgg
     1261 caaagaccgt gaaatggata tgtatttagc taaatatgat gttataggat ctatggctca
     1321 catcacaatg ctcgagagca ttggcttact aacacccaaa gagctaacca ccttgctact
     1381 tggcttaaaa gaaatttata aagaaattga atctggacat tttattatag acgaaaacgt
     1441 ggaagacatc cactctcaag tagagttagt gcttacagat aagttgggtg atatgggtaa
     1501 aaaaattcat agtggacgct cacgcaacga ccaagttcta cttgatttga agcttttttc
     1561 tcgagctaaa atacaggaaa ttgcaacaga aactcactta ctgttcaata ctttattaac
     1621 acaaagtgaa caatataaaa gcgttcttat gcctgggtat acacacctac aaatagctat
     1681 gccttcgtca tttggactat ggtttggggc ttacgcagaa agtttggttg atgatatgca
     1741 actattacta gcagcattta aggtatgtaa tcgcaaccca ttgggttctg ccgctggata
     1801 tggttcttct ttccctctta acagacaaaa aactactgat ttattgggat ttgactccat
     1861 gaattacaat gtagtgtatg ctcaaatggg tagaggaaaa atggagcgta cagtatcaac
     1921 agcattagca tctattgcta acacgatagc caaattggct ttcgatagtt gtttgtttac
     1981 cagtcaaaat tttggattta ttaaattggc tgacgaattt acaacggggt cgagcattat
     2041 gcctcacaaa aaaaacccag atgtgtttga gttaacgcgt gccaaatgca ataaaataca
     2101 aggtattcca caacaaatta atctaattac caacaattta ccttcaggat atttccgcga
     2161 tttgcaaatc atcaaagaat tattcatccc gatgtttgat gagctaatag aatgcatacg
     2221 gatgactcgt tatatcatcg aaaaagtaga aataaacaca cacattttag atgacaagaa
     2281 atatcaagcc atctttagtg ttgaggaagt aaataaacaa gtattatctg gtattccttt
     2341 tagagatgct tacaaaaaca ttggtctcgc catcgaaaag ggagattttc acccagacac
     2401 aagcatttct catacgcatg aaggaagcat tggaaactta tgcaacgaag aaattaattc
     2461 tttaatgaac gagattatat cagcatttaa tttcaaaagt attcaagagg cagaagcgaa
     2521 attactatta taaaaccact cattatgtta ttcagttcaa ttatatttgg tcctataaaa
     2581 agcagaagac taggtaattc acttggaatc aacctgttac ctactgatgc aaaaatttgt
     2641 tcattcgatt gcatttattg tgaatgcgga ctaaatgata gtacaaaaaa aggcaatatt
     2701 cctacacgag aagctgtaaa aaatgctctt tctttagaac tagaaaaact aaaaaaacaa
     2761 gatataacaa tagatactat tacttttgct ggcaatggcg aaccaacaat gcatccagat
     2821 tttaaagaaa ttatagatga cacgttgata atacgtaatc aattagctcc agatgctgtt
     2881 atctctgttc tttcaaatgc atggcacatt accaacccaa aagtatatgg ggctctgcta
     2941 aaagtagaca ataacatctt aaaattggat agtgctataa aagagacaat tttacaaata
     3001 aaccacccca ataatacatc ctttgaggta tcggaactaa ttgcaaattt agcctctttt
     3061 caaggtcaat gtataatcca aactctcttt gtaaaaggaa accataaggg aaatttgatt
     3121 gacaacacaa ccgaagaaga aattaatgca tggctgaatg ccctcaaaac aattcagccc
     3181 aaacaaatta tgatttactc tattgataga gaaacaccta ttaaaagctt agaaaaaata
     3241 cctagttcga cattgaaagc aattgcctca caagccaaaa aactgggatt taatatttct
     3301 gtttcagtat gaaaacagct aagcgaatac taatctgtcc tcttaattgg ggattaggtc
     3361 acgccactcg ctgcattccg ataatacata aattgcaaca agaaggaaat atactactaa
     3421 ttgctagtag tggatgtgca ggagaattac ttcaacaaga atttccaacg cttgaataca
     3481 tacatttccc tggatttagt attacatata gcaaacacca ttcacagact ctacgtattg
     3541 tattgtcatt cccaaaaatt atttattact ctttcaaaga acactttgta ttaaaaaaac
     3601 tcattaaaat acatgatatt aattgcgtaa tatcagataa ccgttttggg ctatggaata
     3661 aagatattac aacagcatac attacccatc agttaaacat taaactacct aaacatgtca
     3721 aaatctttga aaaattaact tggtacatac atcgatattt tatcgaaaaa tataattatt
     3781 gtatcattcc agataatcaa cagagtactt tatcattagc aggagattta gttcaccaac
     3841 gaaaactgcc aaaaaatgct ttcttttgct ctcctctatc acgcttttca aaaagtatcc
     3901 agaatacatc tacaaaattt caatcaaaca ttcttatcat tttatctgga attgaacctc
     3961 aacgttcaat tttggaaaag ctcttattgc aacaacttac caaaataaac caaaaagcca
     4021 tcttgattca aggattgcca aacgaaacta aatacaaaaa aaacataggg aatataacga
     4081 ttatatccta tgttttacac caagaattac aagaattgat taacaataca gatttggtaa
     4141 tttgtcgctc agggtacagt agtatcatgg atctagtagc cataaataaa caagctatta
     4201 taatccctac acctgggcaa acagaacaag aatatttagc aaaatatctg cacgagaaaa
     4261 agttctttat tgctcaacaa caagacaata tagatatttt aaaaggaatg gaagagagta
     4321 aaagcacaaa aattccagat atgaaattct atttacagtt accattttaa ttacaatttt
     4381 tcgccaaaag ccaaatcacc agcatctcct aaacctggta taatatacga atgagcattc
     4441 aattctggat caatagccgc actccataaa gaaatattac tctcctcagc aaaatgattg
     4501 gataaataat caacagcata ctgactcgca ataattgttg caaagtgcac ttccttaggt
     4561 ttccctctgg ttaacaaggc attatatgca agttccatag aagaaccact agcaagcatg
     4621 gggtctacca aaataagcgt tttaccagtc aaatcaggag aagcaatgta ttcaataaaa
     4681 acttcaaact tcaatgcatc cttatatttt ctgtatgcag atataaaagc attatccgca
     4741 ttatcgaaat acgacaaaaa accttgatga aatggtaaac ctgctctaaa tatagtacct
     4801 agcacaatat cattatcaac ggtatttacg ttagctatac ccagtggagt agcaatattt
     4861 ttagttgaat atgctaatga tttactaatc tcataagcca ttacctcgcc aatacgctcc
     4921 atattccgtc taaaacgcat cctatctttt tgaacctctt ttgaacgtat ttgttctaaa
     4981 aaagtattta ataaagagtc ttgcaaagct aaattgtgtg ttctcatact cccatatgtt
     5041 gatttgctaa tttttctttt gctacttcaa tagaatgcaa ctcaagaagt gcaacatatt
     5101 gcactgattt tttatattct tccctccatc cataccattg gtcttcgtct gataatgatt
     5161 cgttatgaaa caatgtaacg aactctccgt tatatttctt aatcacttca gcataataac
     5221 tcataatccc aaacaaatct gtagcttctt tatgcaaata tttttgcaaa gtaacatcca
     5281 ttaaaacaat aggatgcaga cgcatcccag aaatataatc tttggttaaa ttataaaaca
     5341 aaaatggagt gcaagtactc gctctaaagc ctgtatattt tgcgtatcct aaggaataat
     5401 cacgggcaat tgataatgat tcgagatatt caaagctaat tggaaaagta aatttcaaaa
     5461 aatggaatct atttaaaaaa acacgaaccc t
//
LOCUS       DAOSMY010000067         5481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15003_length_5481_cov_3.284372, whole genome shotgun sequence.
ACCESSION   DAOSMY010000067 DAOSMY010000000
VERSION     DAOSMY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15003_length_5481_cov_3.284372"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1686
                     /locus_tag="PLD76_02520"
     CDS             220..1686
                     /locus_tag="PLD76_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU26911.1"
                     /translation="MKTILHKLLIASWLIISPTIAFGRFTTLPTIPEHPSIIGFHIPN
                     NPSAPSGLRNLSLEPKQQLDSIVVAGKEKIVYLYNDNGLHIMLTQYKWNGVSWRPEIQ
                     VDLLYNESNKVATSINYTWNIVLGEWTPQWKTVYTYNVYGQTLTEQYITYDRNTKKWE
                     NIKRYAYTYTNAGVITKSTHANWNTITNTWIVDWTTDYVYDIKKQRIADVSYSEGYVP
                     ASKTEYTYNANGALVRKNDFYSNNTTNRWEPSAKEEYLLDNQLRPVVKTYSFWNSILQ
                     VWENLYKSNVEFDNNGNSLSIINYKWNVEHLRWDITDKQEAQVNASGDITALHYFDWN
                     TATNNWIDRSTHEWNYNDNVTLTMLVLPNYYTNFTNSKHQVTSLVSSYNNNGNEETEI
                     QTYYYSSGNNTTHIMKNDTDAFVKVYYNPTNEYIEVVSDAENNIRFYLYDSKGSMITQ
                     TTLQEGSGKISISQLSPGTYLYTLLNNNEPLRGKLIKQ"
     gene            1922..3391
                     /locus_tag="PLD76_02525"
     CDS             1922..3391
                     /locus_tag="PLD76_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOU26912.1"
                     /translation="MNTQFNFRLVVRVIGALLLVESIILLLVSLVPIFYNEGDAFYFI
                     VSAIISFIIGIILFGLGHHHADKIGKREGSIIVTFTWIFFSLIGLLPYWLSNSIPSFT
                     NAFFETISGFTTTGASILNNIEELSHGMLFWRSLTHWIGGIGIIVISLAIMPVFSTSG
                     TQLFAAETSGPTKDKIHPKIHGTAKRLVLIYVILTFLETILLRFGGMGWFDAICHSFA
                     TIATGGFSTKQSSIAYWDSAYIQYIIILFMILSGINFSLYYFGFKRRFDKILKNEELR
                     YFLLVLISFTLIITFSLFTFSTDTTWQVIEQAFRDALFTVSSLMTTTGFGTADYVLWA
                     PFTWIILLIVMLTGASAGSTSGGIKMIRIVITIKACFYEFKRILHPQAIVPVRYNNLL
                     VSESILNKVLGFLFLYLIVALTGSLILAATGMNFEESIGGMVSCLGGVGPGLGSVGPY
                     GNYAAISDSSKWILSVAMLAGRLELFTFFLIFTPTFWKK"
     gene            complement(3587..4438)
                     /locus_tag="PLD76_02530"
     CDS             complement(3587..4438)
                     /locus_tag="PLD76_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOU26913.1"
                     /translation="MAWVNFHSHTSYCDGIAQPEAYVLQAIELNMPAYGFSGHAPMPY
                     STNWAIKQDQLTSYINEIQRLKEVYTSQIDIYCAVEADYIPQITGPHLFTNYPLDYII
                     GAIHFVGYFADGKPFSVAGKALDFDKGLKEIYGNDIKRLVKTYFSLNCELMLEDTPTI
                     LAHADLIKNGFQDSGIQQEKWYQQALFEMIDVAAERDVIIEINTRGLYKQRTSELYPC
                     EQALRYIKQKGVRMMLSADTHLLTELTSGFEYAVGVLQNNGINEITVFRKGQFVEIPF
                     SSTGFDY"
     gene            complement(4519..4881)
                     /locus_tag="PLD76_02535"
     CDS             complement(4519..4881)
                     /locus_tag="PLD76_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOU26914.1"
                     /translation="MKIAVPVKSTNQIDAHFGHCEGYKVFTIENNNIIAEENVPSPKG
                     CGCKSDIASVLHQKGVTVMLAGGIGDGAVQVLSSNGIQVVRNCKGDANAVLVEYLQGN
                     IVDGGVNCSHDHNHQCSH"
     gene            complement(4900..>5481)
                     /locus_tag="PLD76_02540"
     CDS             complement(4900..>5481)
                     /locus_tag="PLD76_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26915.1"
                     /translation="DKLFTQHYFSKRHSTRSISLAYTFTEEELNRFMPIQQTAHIEAD
                     IKVIAQIQQNYPLPQGNRFLYTQAFDDNEFIQDDFSHEIVVCFGSTSLLVCTGCAHNG
                     LLNMLHTVQNQTSLPIHTVLGGFHLLDADGTYAYETPKEITDIALTLKQTYPSTQFFT
                     GHCTGANSIEILQQTLSSQLEAFYCGWQKQLNY"
BASE COUNT         1804 a          959 c          944 g         1774 t
ORIGIN      
        1 gactaccttt tgttttttta ggatgttttg tttacatatt catttatttt gtaataagat
       61 tgtaaaatct tactgtattt atgttaaata acatgtaaaa atacgtgtaa atgattctgg
      121 tctgatagta tacctattac atttgtatca gaatttcgcc gataatacta tacaaattat
      181 aatttgttag attaagcaaa aaaactaaaa atgaatatca tgaaaacaat actacataaa
      241 ttactaattg caagttggct tattatttcg cctacaattg cgtttggtcg ttttactacc
      301 ttacctacta tacctgaaca tccaagtata attgggtttc acattccaaa caacccttct
      361 gcaccatcgg gattacgcaa tctttctctt gaacccaaac aacaactaga cagtattgtt
      421 gtagcgggaa aagaaaaaat tgtataccta tataatgaca atggattaca tatcatgctt
      481 acacaatata aatggaatgg agtgtcttgg cgaccagaaa tacaagtaga tttgctgtat
      541 aatgaatcga ataaagtagc tacatctatc aattatacgt ggaatattgt gttgggagaa
      601 tggactcccc aatggaaaac agtatataca tataacgtat atggacaaac acttacagaa
      661 caatacatca catatgaccg taacacaaaa aaatgggaaa acattaaaag atatgcttat
      721 acctatacca atgcaggtgt tataacaaaa agcactcatg ctaattggaa cacaataaca
      781 aatacctgga tagtagactg gacaacggat tacgtgtacg atataaaaaa acaacgtatt
      841 gcagatgtgt cgtacagcga aggatatgta cccgcttcta aaacagagta cacatacaat
      901 gcaaatggag ctttagtacg caaaaatgat ttttattcaa ataacaccac caatagatgg
      961 gaaccatcgg caaaagagga atacctattg gataatcaac tacgaccagt tgtaaaaaca
     1021 tatagttttt ggaacagcat attgcaagta tgggaaaacc tatataaaag caatgttgaa
     1081 tttgataaca atggaaatag cttatcaatc attaattata aatggaatgt tgagcatttg
     1141 cgttgggata ttactgataa acaagaagct caggtaaatg ccagtgggga tattacggca
     1201 ttacattatt ttgattggaa tacagcaacc aacaattgga tagatagaag tacgcatgaa
     1261 tggaattaca atgacaatgt aaccttaacc atgttagtgt tacccaatta ctataccaat
     1321 tttacgaata gcaaacacca agtaacgtcg ttagtatcat cctataataa caatggaaac
     1381 gaggaaactg agattcaaac gtattattat tcgtctggca acaacacaac acacatcatg
     1441 aaaaacgata cagacgcatt cgtaaaagtc tattacaatc caactaatga gtatatagaa
     1501 gtggtgagtg atgctgaaaa caatatacga ttctacttat acgatagcaa aggatctatg
     1561 attactcaaa cgactttaca agaaggttca gggaaaatat ctatatctca attatcacca
     1621 ggaacgtatc tatacacctt actcaacaac aacgaaccct tacgtggtaa attgataaaa
     1681 cagtaaaaaa cactgtaatt tatacaagaa aaacaggcat accagcatgc ttgtttttct
     1741 ttttgtcaaa agaccaaatt aaattcaatt tttattgtac agataatgta aaacgaatat
     1801 ctttgtcagc ctttctttat gctctcagag aaatacattg tgcattctct ctagcatttc
     1861 caaagacccc tttttgtcaa aaaaaatggt aaaataaaca ctctttatta atcgttggat
     1921 aatgaatact caatttaatt ttcgattggt tgtacgagtt attggagcat tattgttggt
     1981 agaatctata attttacttc tagtaagcct tgttcccatc ttctataatg aaggagatgc
     2041 tttttatttc attgtatctg ccattattag ttttattatt gggattatat tatttgggtt
     2101 aggacatcat catgctgaca aaataggcaa gagagaagga tccattattg taactttcac
     2161 atggatattt ttttccttaa ttggattgct cccttattgg ttaagcaact ccattccttc
     2221 atttacaaac gctttttttg aaacaatttc gggttttaca actacaggag cttccatttt
     2281 aaataatatt gaagaattat ctcatgggat gttattttgg cgaagtttaa ctcactggat
     2341 tggtggtatt ggcattatcg ttatttcgtt ggcaatcatg cctgtattta gtacaagtgg
     2401 cacacaatta tttgcggcag aaacttctgg accaacaaaa gacaagatac atcccaaaat
     2461 acatggaaca gcaaagcgtt tagttttaat ttatgtaata cttacatttt tagaaacgat
     2521 actgttgcgt tttggaggta tgggctggtt tgatgcaatc tgccattcgt tcgcaactat
     2581 tgctacaggg ggattctcca caaaacaatc aagcatagcc tactgggact ccgcctatat
     2641 ccagtacatt attattcttt ttatgatatt atcaggcatt aacttcagtt tatattactt
     2701 tggttttaaa agacgatttg ataaaattct aaaaaatgaa gaattacgtt actttttact
     2761 tgtattaata agctttaccc ttattattac gttttcatta tttactttct ccacagatac
     2821 aacatggcaa gttattgaac aagcatttag agatgcattg tttacggtat caagtttaat
     2881 gactactaca ggttttggca ccgcagatta tgttttatgg gctcctttta catggattat
     2941 tttactcatt gtaatgctta caggagcttc ggctggttcg acaagtggtg gtattaaaat
     3001 gatacgcatt gtgattacca taaaagcttg tttctacgag tttaaaagaa tactacatcc
     3061 tcaagcaatt gtacctgttc ggtataataa tttgctagta agtgaaagta tcctgaataa
     3121 agtattagga tttttatttt tataccttat agtggcacta acgggttcac ttatattggc
     3181 tgctacaggc atgaattttg aagaatctat tggtggtatg gtttcttgct tgggaggagt
     3241 tggaccaggg ttaggctctg ttggaccata tggtaattac gccgctattt cagatagcag
     3301 taaatggata ttatcggtag caatgctggc tgggagactt gaactattta cctttttctt
     3361 aattttcaca cctacctttt ggaaaaaata ggtctacatc acgtcaaatt gtaacagctt
     3421 tgtaaaatct ctataaaaat ctgttaattt atagacaaag agtaagctcg tattgccaga
     3481 aactacaaaa tcatagtatt tttgcttatg aatttttcat agtatttgat ttttaaggtt
     3541 agcaagtagg cagtcaggcg agactgcctt ttgtgttttt ataccatcaa taatcaaacc
     3601 cggtagaaga aaaaggaatt tccacaaact gccctttacg aaatacagta atctcgttga
     3661 tcccattatt ttgtagtact cctacggcat attcaaaacc cgaagttagc tctgtaagta
     3721 aatgagtatc ggcactgagc atcattctca cccctttttg tttgatatac cgcaatgcct
     3781 gttcgcaggg gtatagttct gaagtacgtt gtttgtatag tcctcgtgta ttaatttcga
     3841 taattacatc tcgttcagcc gccacatcaa tcatctcgaa caaagcctgt tggtaccatt
     3901 tttcttgttg aataccactg tcttgaaacc catttttaat taagtcggca tgtgctaaaa
     3961 ttgttggtgt atcctcgagc attagttcac aattgagcga aaaataggtt tttaccaagc
     4021 gttttatatc gttgccataa atttctttta gtcctttatc aaaatctaag gctttgccag
     4081 ctacgctaaa aggttttcca tcagcaaaat aaccaacaaa atggatagct ccaatgatat
     4141 aatctaaggg ataatttgta aacagatgcg gacctgttat ttgtggaata taatcagcct
     4201 ctactgcaca ataaatatct atttgggacg tatatacttc tttaagtcgc tgtatttcat
     4261 taatatacga tgttagttga tcttgtttta tcgcccaatt tgtactatat ggcataggag
     4321 catgaccaga aaatccatag gcaggcatgt ttagctcaat agcttgcaac acatacgcct
     4381 ctggctgtgc tatcccatcg caataagaag tgtgtgaatg aaaattgacc caagccatac
     4441 taaaactatt agctacaaag atacggtaat tttcacttta caaaaagtga acacatatca
     4501 aaataaaaaa atatattctt aatgagagca ttgatggttg tgatcgtggc tacaattcac
     4561 tccaccatcc acaatattgc cttgtaaata ctctaccaaa acagcattgg catctccctt
     4621 gcaattacgc accacttgta tcccattgct tgataacacc tgtacagcac catcgccaat
     4681 accaccagca agcattactg taacgccttt ttggtgaaga acactggcaa tgtccgattt
     4741 gcatccacag cctttggggg aaggaacatt ttcttcagca ataatattgt tgttctcaat
     4801 agtaaatact ttgtagcctt cgcaatgacc aaaatgagca tcaatttgat tagttgattt
     4861 tacaggaacg gcaattttca tacgattata gttttaaaat tagtaattta gttgtttttg
     4921 ccaaccacaa taaaaggctt ccaactgact cgaaagtgtt tgttggagta tttcaataga
     4981 attggctccc gtacaatgtc cagtaaaaaa ctgggtggag ggatatgttt gttttaatgt
     5041 aagagcaata tcggttattt cttttggtgt ttcgtaagca tacgttccat cggcatctaa
     5101 cagatgaaat cctcctaaca cagtatgtat gggcaacgat gtttgatttt gtactgtatg
     5161 caacatattc agcaaaccat tgtgggcaca tcctgtacac acaagcaacg aagttgaccc
     5221 aaaacacacc actatctcgt gcgagaaatc gtcctgtata aattcattat catcaaaggc
     5281 ttgagtatat aaaaaacgat tcccttgtgg caatgggtag ttctgttgta tctgtgcaat
     5341 tacttttata tccgcctcaa tgtgagcagt ttgttgaatt ggcataaaac gattcagctc
     5401 ctcttcggta aaagtatatg ctagactaat agagcgtgtg gaatgacgtt tagaaaaata
     5461 atgttgcgta aagagcttgt c
//
LOCUS       DAOSMY010000068         5435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15150_length_5435_cov_3.137918, whole genome shotgun sequence.
ACCESSION   DAOSMY010000068 DAOSMY010000000
VERSION     DAOSMY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15150_length_5435_cov_3.137918"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLD76_02545"
     CDS             <1..674
                     /locus_tag="PLD76_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Por secretion system protein"
                     /protein_id="HOU26916.1"
                     /translation="IAEDKDGTVWLGTDKGPLLMTNVSKVFDNTYTCTRVKIPRNDGT
                     ILADYLLDNEEIRAIAIDAANRKWIGTGNSGVYLLSANGRETIHHFTVDNSPLSSNKI
                     ESIVIQEETGEVFFSTQDGLISYRSDATVGKKSFEKVEVFPNPIRPDFQGLITVRGLV
                     ANTEVRIVDQSGLLVFSGVATGGSIVWDGMNFANHPVGSGVYYVFSVNADGTEHGSTK
                     FMIIR"
     gene            complement(671..1144)
                     /gene="folK"
                     /locus_tag="PLD76_02550"
     CDS             complement(671..1144)
                     /gene="folK"
                     /locus_tag="PLD76_02550"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU26917.1"
                     /translation="MNVVYLALGSNIGNKKRILSEAESLINEKIGTVIHASSVHTTNP
                     EGFISTHKFLNKVICVYSILSPQKILETILEIEKKLGRNAKSIDGNYQDRTIDIDILY
                     YNDSIINVETLTVPHPHLHTRVFVLKPLLEIAPELIHPILQLSTKQMLLRLEKED"
     gene            complement(1150..2949)
                     /gene="typA"
                     /locus_tag="PLD76_02555"
     CDS             complement(1150..2949)
                     /gene="typA"
                     /locus_tag="PLD76_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOU26918.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMLLAGSLFKEHEKVGELMMDN
                     NDLERERGITIVSKNVSIRYKDYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIQIGLKPIVVINKVDKPNCRPDEVHEAVFELMFSLDATEEQLDFP
                     TIYGSAKQNWMSDSWSKQTDTIFPLLDAIIEHIPAPKKLEGTPQLLITSLDYSSYVGR
                     IAIGRIHRGTLKENMDVALVKRDGSIVKSRIKELNVFEGFNKLKVPSVSSGEICALIG
                     IDNFDIGDSITDLEKPDPLPPIAIDEPTMSMVFTINDSPFFGKDGKFVTSRHIHERLM
                     KELDRNLALQVVKSETADSWNVYGRGVLHLSVLIETMRREGYELQVGQPQVIFKTIDN
                     VKHEPIELLTINVPEEVSGKVIELVAVRKGEMTIMERKGDRVHMEFMIPSRGIIGLRT
                     NMLTATAGEAVMAHRFIEYAPYKGEIERRSNGSIIAMESGTAFAYAIDKLQDRGKFFI
                     FPQDEIYAGQVVGENSKEGDMVVNVTKSKKLTNMRASGSDDKVRIVPPIVFSLEEALE
                     YIKEDEYVEITPNSMRLRKILLDENDRKRAKAK"
     gene            complement(3014..3847)
                     /gene="murI"
                     /locus_tag="PLD76_02560"
     CDS             complement(3014..3847)
                     /gene="murI"
                     /locus_tag="PLD76_02560"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU26919.1"
                     /translation="MRKGSRISRIGVFDSGYGGLTILEEFRKKLPEYDYVYLGDNART
                     PYGTRSFEVVYQYTLECVKKLAKMDCELIILACNTASAKALRTIQQKDLQILFPQKRI
                     LGVIRPTVEQLDTCTQSKHVGILGTEGTIQSQSYTIEINKLYPHITVVDQACPMWVPL
                     IENNEHESDGADFFVHKYLSNLLNKDKFIDAIVLGCTHYPLLLKKIKHILPSHINIIS
                     QGEIVAQSLENYLLRHPEIETKLAKNHQCMFYTTESELKFISSASIFLKETIKAERIS
                     L"
     gene            complement(3916..4434)
                     /locus_tag="PLD76_02565"
     CDS             complement(3916..4434)
                     /locus_tag="PLD76_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU26920.1"
                     /translation="MKKSIIISLCIAFSFSLFAQTNDVPKFGHINKQDVFSLMPEADA
                     AMKELEALLLNYKVESKKLEEEFTKKQQDYVANQATLDAAIKKYRETELQRLYASIEE
                     FSKNADEALKKRQSELLAPIEAKINDAIKTIGDENGFIYIFDVTTPGTFAYMSTKSVD
                     ILPLVKQKLNLK"
     gene            complement(4468..4983)
                     /locus_tag="PLD76_02570"
     CDS             complement(4468..4983)
                     /locus_tag="PLD76_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU26921.1"
                     /translation="MKKTLLLVGIFVLSFTAMQAQRFALVDIEYILQQIPTYESATEQ
                     LNQSSKQWEKEIEALTTEAQTLYKNYQTEMVFLSDEMKVKREEAIIAKEKEAMDLKKK
                     YFGAEGELFKKKESLLKPIQDEIYNAVKEISENKNIQLILDRASGNNIIFASPTIDIS
                     DEVLQRMGYVR"
     gene            complement(5005..>5435)
                     /locus_tag="PLD76_02575"
     CDS             complement(5005..>5435)
                     /locus_tag="PLD76_02575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU26922.1"
                     /translation="TKAKADQFKFPYSKSYFDIVVLTSVFTHMMPNDVANYIEQISDV
                     LKPEGMCYATFFLFNKQENFSSTERSFQFSYDYGHYRLLDKKVKEANIAFDEAFIHSL
                     AEKHQLSIKHSIKGWWRGIEKTDKIDFQDIIIFEKRNQLS"
BASE COUNT         1583 a          941 c          911 g         2000 t
ORIGIN      
        1 cgatagctga agataaagat ggaacagtgt ggcttggaac agataaaggt ccattgttga
       61 tgaccaatgt gagcaaagta tttgacaaca catatacatg tactagggtt aaaattccta
      121 ggaatgatgg tacaatattg gctgattatt tattagataa tgaagaaata cgtgctatag
      181 caattgatgc agctaataga aaatggatag gcacaggcaa ttcaggagta tatctattgt
      241 cagcaaatgg tcgggaaaca attcatcatt ttactgtaga taatagtcca ttgtcttcga
      301 ataaaattga atccatcgtt attcaagaag aaactgggga agtgtttttt tctacacagg
      361 atggtctaat ttcttatcgt tcagatgcta cagtaggaaa aaaaagtttt gaaaaagtag
      421 aagtatttcc taatcctata cgacccgatt ttcaggggct aattactgtt agagggttag
      481 ttgccaacac agaagttcgt attgttgatc agtcaggttt gttggtcttt tctggtgtag
      541 ctactggggg ttcaattgtg tgggatggga tgaattttgc caatcatcct gttggctctg
      601 gtgtatatta tgtattttct gttaatgccg atggcacaga gcatggctca actaagttta
      661 tgattattcg ttagtcttcc ttttcaagtc ttaaaagcat ctgttttgta gatagttgaa
      721 gaattggatg tatcaattct ggggctattt ctaaaagggg ttttaataca aacactcgtg
      781 tatgtaaatg tggatgagga actgttaggg tttcaacgtt aataatagaa tcattataat
      841 ataaaatgtc aatatcaatt gttctatctt ggtagtttcc atctatagat ttggcattcc
      901 ttccaagttt tttttcgatt tcgagaatgg tctctaagat tttttgtgga cttaaaatag
      961 aatacacaca aattacttta ttaagaaatt tgtgtgtaga aataaatcct tcagggtttg
     1021 ttgtatggac tgaagacgca tgtataactg taccaatttt ctcgttaata agggattcag
     1081 cttccgatag aattcgcttt ttattcccaa tattacttcc tagagctagg tatacgacat
     1141 tcattgattt tattttgctt tcgctctttt tctgtcattt tcgtcaagaa gaatttttcg
     1201 caatcgcatt gaatttggtg ttatttcaac atattcatct tctttaatgt attctaaagc
     1261 ctcttctaaa ctaaatacaa taggaggaac gatgcgtact ttgtcatccg aaccagatgc
     1321 acgcatattc gttagtttct tcgatttcgt tacatttacc accatatctc cttctttaga
     1381 attttcacca actacttgtc cagcatatat ttcgtcttga ggaaaaataa agaactttcc
     1441 tctatcttgt aatttgtcaa tagcatacgc aaatgcagta ccactttcca ttgcaataat
     1501 tgatccattg cttcttctct caatttcacc tttgtaagga gcatattcta taaaacgatg
     1561 agccattaca gcttcacctg ctgtagccgt tagcatattg gttcgtaagc caataatccc
     1621 cctagaggga atcataaatt ccatgtgtac ccgatcacct ttacgttcca taatagtcat
     1681 ttctccttta cgcacggcaa ctaactcaat tactttacca gaaacttctt ctggtacatt
     1741 tatggttaat aattcaatag gttcatgttt tacattatca attgtcttaa aaataacctg
     1801 tggctgtcct acttgtaatt catatccttc gcgtcgcatt gtttcgatta atacggataa
     1861 gtggagtact ccacgtccat atacgttcca cgaatcggct gtttctgatt ttacaacttg
     1921 taatgctaag tttctgtcta attctttcat caaacgctcg tgaatatgcc gtgaagtaac
     1981 aaattttcca tccttaccaa aaaatggaga gtcgttaatt gtaaatacca tactcattgt
     2041 aggttcgtcg atagcgatag gaggtagcgg atctggtttt tcaagatcag ttatgctatc
     2101 gccaatatca aaattatcaa taccaattaa ggcacaaatt tcacctgagg atacacttgg
     2161 tacttttaat ttgttaaacc cttcaaatac atttaattct tttattcgag attttacgat
     2221 acttccatcg cgtttaacta aagccacatc catattttct tttaatgtgc ctctatgaat
     2281 gcgtccaatt gctatacgac ctacatacga tgaataatct aaggatgtta ttaataattg
     2341 aggggtacct tccagttttt taggagcagg aatatgttca atgatagcat ctagtaaagg
     2401 gaaaatagta tctgtttgtt tggaccaaga gtcagacatc cagttctgtt ttgcagagcc
     2461 ataaatagtt gggaagtcta gttgttcttc tgtagcatcc aaactaaaca tgagttcgaa
     2521 tactgcttcg tgtacttcat ctgggcgaca atttggttta tctactttgt ttattacaac
     2581 gattggcttt agacctattt gaatggcttt ttgcaacacg aaacgtgttt gtggcattgg
     2641 accttcaaat gcatccacta atagcaacac accatcagcc atatttaaca cacgttctac
     2701 ttcaccacca aaatcggagt gaccaggagt gtcaatgata ttaattttgt aatctttata
     2761 gcggatagaa acatttttag atacaattgt aatgcctcgt tctcgctcaa ggtcattatt
     2821 atccatcatt aattctccca ctttttcgtg ttctttgaac agtgaacccg ctaataacat
     2881 tttatcaact aatgttgttt ttccgtggtc aacgtgtgca ataatcgcaa tatttctaat
     2941 cttttgcata tagtttttat ttgaggatgc aaaggtagtc agaatatttt tatctgtact
     3001 ataattttga ttattacagc gaaattcttt ctgcttttat cgtttctttt aagaagatgg
     3061 aggcggagga aataaattta agctcagatt cagtagtata aaacatacat tggtggtttt
     3121 tagctaattt tgtctctatt tcaggatgac gcaaaagata gttctctaaa ctttgagcaa
     3181 caatttcgcc ctgagagata atattgatat gtgacggtaa gatgtgctta atttttttca
     3241 ataacaatgg gtaatgtgta catcccaata caatcgcatc aataaatttg tctttgttaa
     3301 gtaagtttga aagatactta tgcacaaaaa aatcagcacc atcactttca tgttcattgt
     3361 tttcaataag aggcacccac atcggacaag cttgatccac tactgtaata tgcggatata
     3421 gtttatttat ctctatagta tacgattgag attgaatggt gccttccgtt cccaaaatcc
     3481 caacatgttt agattgggtg caagtgtcta gttgttctac tgttgggcga attaccccta
     3541 gtattcgttt ttgcggaaat agtatttgca agtctttttg ttgaatcgta cgtagtgctt
     3601 ttgccgaagc tgtattgcaa gccaaaataa ttaactcaca atccatcttg gctagtttct
     3661 ttacgcattc cagtgtgtac tgatacacta cttcgaacga acgtgttcca tatggcgttc
     3721 gagcattatc tccaaggtaa acataatcat actcgggtag tttttttcta aactcctcta
     3781 agatagttaa tcctccataa ccagagtcaa aaacacctat acgggatatt ctacttcctt
     3841 tacgcatata aaacaacagg aagatagagt acactatttg tctacttttc ttcctgtttg
     3901 ctatcagtaa attgtttatt ttaaattcaa cttttgtttt actaatggta aaatatctac
     3961 acttttggtc gacatataag cgaatgtacc aggtgtggtt acatcaaaaa tatagataaa
     4021 tccgttttcg tctccaatcg ttttgatagc atcatttatt tttgcctcaa tcggtgctaa
     4081 taattctgat tgtcttttct ttagggcttc gtctgcattt tttgaaaatt cttcaatact
     4141 tgcatataaa cgttgtaatt ctgtttctct atattttttt atagcagcat caagtgttgc
     4201 ttgatttgca acataatctt gttgtttctt tgtaaattct tcctctagct ttttactttc
     4261 tactttgtag ttaagcaata aagcttccag ctctttcatt gctgcatcgg cttctggcat
     4321 taaagaaaag acatcttgtt tattgatatg accaaatttt ggtacatcat ttgtttgtgc
     4381 aaataaagaa aaagaaaatg cgatacataa gctaataatg atgctttttt tcataatgtt
     4441 tttttatttt aatgagtaaa attggtttta tcgtacatat cccatgcgtt gtaatacttc
     4501 gtcgctaata tcaatagtag gggatgcaaa aataatatta tttccagacg ctctgtccaa
     4561 aattaattga atatttttat tttctgagat ttcttttaca gcattataaa tctcatcttg
     4621 aataggtttg agaaggcttt cttttttctt aaatagttct ccttcagcac caaaatattt
     4681 ctttttcaaa tccattgctt ctttttcttt tgcaatgatt gcttcttctc tttttacctt
     4741 catttcatct gataaaaata ccatttctgt ttgataattt ttatacaatg tttgagcttc
     4801 agtggtgaga gcttcgattt ccttttccca ttgttttgaa ctttggttaa gttgttctgt
     4861 agcagactcg taagttggta tttgttgcaa tatatattcg atatctacca atgcaaatcg
     4921 ttgagcctgc atcgctgtga aactaaggac aaaaatgcct actaatagaa gtgttttttt
     4981 catgtctttt acattttaag attactaact aagttgattt cttttttcaa aaataataat
     5041 atcttgaaaa tcaattttat ctgttttttc tatacctcgc caccaacctt taatactgtg
     5101 tttaatacta agttggtgtt tctcagctaa agagtgaata aatgcttcat caaatgcaat
     5161 attagcttct tttacttttt tatcaagcaa gcgataatgt ccgtagtcgt aactaaattg
     5221 aaagctcctt tctgttgagg agaaattttc ttgtttatta aataggaaga atgtggcata
     5281 acacatccct tctggtttta atacatcact aatctgttct atataattag caacatcgtt
     5341 tggcatcata tgtgtgaata ccgatgttag aactacgata tcgaagtacg actttgaata
     5401 aggaaattta aattgatcag cttttgcttt ggtgg
//
LOCUS       DAOSMY010000069         5374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15359_length_5374_cov_3.399699, whole genome shotgun sequence.
ACCESSION   DAOSMY010000069 DAOSMY010000000
VERSION     DAOSMY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15359_length_5374_cov_3.399699"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLD76_02580"
     CDS             complement(<1..633)
                     /locus_tag="PLD76_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOU26923.1"
                     /translation="MNTLDNQQGTEESLSKSIEPADKEVSITTLSTEGEPTSVGHTNH
                     EESTFVSLEDLLTALELAIKMPNAKNEITRIKAEFYKQLAINNAKEKAAFDAKHIDEG
                     LIFEPKIGVEEERFKQLLATYKEKRNQEIQQQEQSKEDGLLKKESLLARLQMVIESTD
                     DVSKSFPVVQEIQQEWRSIGAIASEKYKPLVRKYQLLMEQFYDFVKISNDL"
     gene            917..3346
                     /gene="thrA"
                     /locus_tag="PLD76_02585"
     CDS             917..3346
                     /gene="thrA"
                     /locus_tag="PLD76_02585"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOU26924.1"
                     /translation="MKVLKFGGTSVGSISNIFQIKNIINAIDEPVIIVVSAVGGITDK
                     LIHTANKAANGNKEYLADLQEIISIHHQIIEGVVEDNDKNTVLATVNHFFDELANIYK
                     GVYLIKDLSVKTQNVVVSYGEKISSYFLSFALNATLYNACDFIKTETILGKRAVDYTT
                     TNRLVKELFVNHPNRCIVGGFIASCSKTNKVTNLGRGGSDYTAAILAAALDARKLEIW
                     TNVDGFLTADPRLVPNAYTILEMSYIEAMELSNFGAKVIYPPTIYPVYAKNIPVYIKN
                     TSNPEIKGTKITNKKNSGDKTIKGISSIQDTCLITIQGMGMVGVLGISSRTFKTLADA
                     GINVFLISQASSENSTTFAVKHKDADAAVDVLKREFEKEIEKGSIEKIVPEKNLATIA
                     IVGENMKHNTGIAGKLFNTLGRSGINIIACAQGASETNISFVVSMGNLKKSLNVIHDA
                     FFLSDYQALNIFLVGTGTVGGSLLEQIHQQQSKLLEQNSVKLNVIGITDLNGSIFVPQ
                     GIDLSNYMEQIHQNGKQLNPEQVCDTIIEMNMFNSVFVDCTASPQIANLYEKLLNNNI
                     SVVAANKIAASSDYDHYMHLKSLARKKGIKYLFETNVGAGLPIINTINNLINSGDKIL
                     KIEAVLSGTLNFIFNTISEKISFSQSIQMAKEARYAEPDPRIDLSGKDVIRKLVILAR
                     ESGYKIDQDDVAKNLFVPDSYFQGSVDDFWNTIGELDAEFEEKRKKLAAQDKKYRFVA
                     KMDNGKCSVGLQEVDARHPFYDLEGSNNIIMIHTERYFEYPMIIKGYGAGASVTAAGV
                     FGDIISIANIR"
     gene            3362..3907
                     /locus_tag="PLD76_02590"
     CDS             3362..3907
                     /locus_tag="PLD76_02590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU26925.1"
                     /translation="MLVNSKKNWWIMACILLLASCTEPTPQNPTNKLVSTDSVQQNLL
                     LLNQAFVALEQQEINEYIAQHRLNVHLSENGYWYRIDKRGTGNTLKANQLVTIEYYLE
                     LLDGTLCYSSEKNGIKQFRVGKFEVEKGLDDFMLLLLLGAEATCIIPSYLAHGVVGDR
                     NCIPPRSPIVYRIKVISIKDN"
     gene            3969..4508
                     /locus_tag="PLD76_02595"
     CDS             3969..4508
                     /locus_tag="PLD76_02595"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOU26926.1"
                     /translation="MKLYNTLLFFSISIVLVFTQCTSEETYAQKLKKERLKFAEYVEK
                     GNIQLSSDSIYCFSLPTPWPENLYYRTPRGAYINLIDKGTSKEATEGSTVIVRYVATN
                     IAGDTIANNTSTSVSRDGYEFIYKEGSSVPCIGWNDAMLYLNNNGKAKVIVESKIGFS
                     QHQTSVITVISDVTVSITN"
     gene            4526..>5374
                     /locus_tag="PLD76_02600"
     CDS             4526..>5374
                     /locus_tag="PLD76_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOU26927.1"
                     /translation="MTLIKSISGIRGTIGGKAGEGLSPLDIVKFTAAYATYILKNTTK
                     KTNKVVVGRDARISGEMVNNIVNGTLMGMGIDVVNIGLATTPTTELAVTMENAIGGII
                     LTASHNPTQWNALKLLNEKGEFLDDKAGKAILEIANDESFQFADVLELGKLTENNTFN
                     QKHIDSVLSLKLVDVDAIRNAKFRVAIDCVNSVGGIIIPQLLSALGVTEIVPLYCEPN
                     GKFPHNPEPLPEHLTDIASILTNGKADVGFVVDPDVDRLAIISENGEMFGEEYTLVSV
                     SDYVLQH"
BASE COUNT         1872 a          854 c          936 g         1712 t
ORIGIN      
        1 taaatcatta ctgattttaa caaaatcata aaattgctcc atcaacaatt gatattttct
       61 aaccaaaggt ttgtattttt cagaagctat tgcaccaata gaacgccatt cttgctgtat
      121 ttcttgcacc actggaaatg atttacttac atcatctgta ctctcaatca ccatttgtaa
      181 tcttgctagt aacgattctt ttttaagcaa accatcctct ttggattgtt cttgttgctg
      241 aatttcttga tttctctttt ccttgtatgt agccaataat tgcttaaaac gttcttcctc
      301 tacacctatt ttaggttcaa aaattaaccc ttcatcgata tgctttgcat caaacgctgc
      361 tttttcttta gcattattta tcgctagttg tttgtaaaac tctgctttaa tgcgagttat
      421 ttcattttta gcgtttggca ttttaattgc caattctaat gctgttaaaa gatcctccaa
      481 actaacaaat gtggactctt cgtgattagt atgtcctact gaagttggct ctccttctgt
      541 tgatagagta gttatagata cctccttatc agcaggttca atgctttttg ataaggactc
      601 ttctgttcct tgttggttgt caagtgtgtt catagcaaat atggaactaa atcaaaaata
      661 aaatttacaa tcgacaaaga tacaagtttt tttttagaga cttcattttt tctggacata
      721 atcaataaaa gcacaaaaaa ttgggttata tatttcagta atcataaaaa aatgagtaat
      781 attgcaagtt aatttagaaa agacataata aaacacaatc tattggcaca ataagtacat
      841 ctactcagca tacaaacaga gtctttgtta cattatttta ttcggtagtg cgtaaaataa
      901 acacaatagt aaaaatatga aagtattaaa atttggagga acatccgtag gttccatttc
      961 aaacattttt caaattaaaa atatcatcaa tgccattgat gagccagtta tcattgtagt
     1021 ttctgcagta ggaggcatta cggataagtt aatacataca gccaataaag cagcgaatgg
     1081 aaataaagaa taccttgctg atttgcaaga aattatcagc atacatcatc aaattattga
     1141 gggagtagtt gaggataatg ataaaaacac agttttagct acagtcaatc acttttttga
     1201 cgaattagca aatatttata aaggagtgta tttaatcaaa gatttatctg taaaaactca
     1261 aaatgtagtt gttagttatg gagaaaaaat ctcatcctac tttttaagtt ttgctttaaa
     1321 tgctacttta tataatgcct gcgattttat taaaacggag acaatcttgg gtaaaagagc
     1381 tgttgattat accactacca acagattggt aaaagaactt ttcgtaaacc atccaaacag
     1441 atgtattgtt ggtggattta ttgcttcgtg tagcaaaacg aataaagtaa ccaacttagg
     1501 cagaggcggt tctgactata ctgctgcaat tttagcagcg gctttagatg ctcgcaaatt
     1561 agaaatatgg acaaatgtag atggttttct tactgccgac cctagattag tgccaaatgc
     1621 ttataccatt ttggagatga gttatatcga agctatggag ttgtccaatt ttggagctaa
     1681 agtaatatat cctcctacaa tctatcctgt atatgcaaaa aacatacctg tatatattaa
     1741 aaacacaagc aatcctgaaa taaaaggcac aaaaattact aataagaaaa actctggaga
     1801 taaaactata aagggtattt catctataca agacacctgc ttaattacca tacagggtat
     1861 gggtatggtt ggtgtattgg gcataagttc acgcactttt aaaacactag cggatgctgg
     1921 aattaatgta tttttgatat cacaagcctc gtcggagaac tctacaactt ttgcagtaaa
     1981 acataaagat gcagatgctg ctgttgatgt attaaaacga gaatttgaaa aagagataga
     2041 aaaaggtagt attgaaaaaa ttgttcctga aaaaaactta gcaaccatag ctattgttgg
     2101 tgaaaatatg aaacacaaca cgggtatagc tggcaagcta ttcaacaccc ttggtagaag
     2161 tggcattaac atcattgcgt gtgctcaagg tgcatcagaa acaaatattt cgtttgttgt
     2221 gagtatgggt aatttgaaaa aatcgctcaa tgttattcac gatgcattct ttttgtctga
     2281 ttatcaagct ctcaatatat ttttagtagg aacaggaact gtgggtggta gtttactcga
     2341 acaaatacac caacaacaaa gtaaactttt agaacaaaat agtgtaaaac taaatgtgat
     2401 tggaataact gatttaaatg gttcaatatt tgttccacaa ggaattgatt tatcaaacta
     2461 tatggaacag attcatcaaa acggaaaaca actaaatcct gaacaagtat gcgataccat
     2521 cattgaaatg aatatgttta attctgtttt tgttgattgt actgctagtc cacaaattgc
     2581 aaacctatac gaaaaacttc ttaataataa catctctgtt gtggctgcca ataaaatcgc
     2641 ggcatcatcc gactatgatc attatatgca cttaaaatct ttggctcgca aaaaaggaat
     2701 taaataccta tttgaaacca atgttggtgc tggattaccc attattaaca ctatcaacaa
     2761 tttaattaat agtggggata agatattaaa aattgaggct gtactttctg gtacattaaa
     2821 ttttattttc aatactataa gcgaaaagat atctttctct caatctatcc agatggcaaa
     2881 agaggctcgc tatgctgaac ctgatcctcg cattgattta agcggaaaag atgtaattcg
     2941 taaattggta atcttagctc gcgagtcagg ctataaaata gatcaagatg atgtggcaaa
     3001 aaatttgttt gttcctgatt cgtattttca gggttcagtt gatgattttt ggaatactat
     3061 tggcgaatta gatgctgaat ttgaagaaaa gcgtaaaaaa ttagctgctc aagacaaaaa
     3121 ataccgcttt gtagcaaaaa tggataatgg aaaatgctca gtaggtttgc aagaagtgga
     3181 tgcacgacac ccattttacg atttagaagg gagcaacaat atcattatga tacatactga
     3241 acgttatttt gaatatccaa tgatcattaa aggatatggt gctggtgcta gtgttacagc
     3301 agctggcgta ttcggggata ttattagtat tgctaatatt cgataagaaa aaacatctct
     3361 aatgttggtt aatagtaaaa aaaactggtg gataatggca tgtatactgc ttcttgctag
     3421 ttgtactgag cctactccac aaaacccaac caataagcta gtatcaacag atagtgtgca
     3481 acaaaactta ttacttttaa atcaagcctt tgtggctctt gaacaacaag aaataaacga
     3541 atacattgct caacataggt taaacgtaca tttgtcagaa aatggctact ggtatcgtat
     3601 agataaacga ggtacaggaa atacgcttaa agccaatcaa ttagtaacta ttgaatatta
     3661 ccttgaacta cttgatggta ccctttgtta ttcttcagaa aagaacggta taaaacaatt
     3721 tagggtgggt aaatttgaag tagaaaaagg attagatgat ttcatgttat tactcttatt
     3781 gggagccgaa gctacatgta tcattccttc gtacttagct catggtgtgg ttggtgacag
     3841 aaattgtata cctcctcgtt caccaattgt atatagaatc aaagtaataa gtattaaaga
     3901 taattaatac tcaatcataa cacaattttt atgtgtactt ttgcgtacgt ttatttaaat
     3961 atcattttat gaagttatac aacacacttt tatttttctc tattagcata gtacttgttt
     4021 ttacacaatg tacttcggaa gaaacatatg cacaaaaact taaaaaagaa cgcttaaaat
     4081 ttgccgaata tgtagaaaaa ggaaatattc agttatcatc tgattctatt tattgttttt
     4141 ctttacccac cccatggccc gaaaatttat actatagaac acctcgaggg gcatatatta
     4201 acctcataga taaggggact tcaaaagaag cgacagaagg ttcaaccgta attgtaagat
     4261 acgtagcaac caatatagct ggagacacaa ttgcaaataa cacctctaca tctgtaagta
     4321 gagatggata tgaatttatt tataaagaag gaagtagtgt accttgtata ggatggaatg
     4381 acgctatgtt gtatctgaat aacaatggta aagcgaaagt tattgttgaa tctaaaattg
     4441 gattttcgca acaccaaact tctgttatta cagtaatttc tgacgtgact gtatctatta
     4501 caaattaata tacaaacaca gaattatgac attaataaaa tctatttcag gtattagagg
     4561 taccatagga ggaaaagctg gtgaagggtt gagtccattg gatatagtaa aatttacagc
     4621 tgcttatgcc acatacatcc ttaaaaacac tacaaaaaaa accaataaag tggtagtagg
     4681 tagagatgct cgtatttcgg gtgaaatggt taataatatt gtaaatggca cattaatggg
     4741 tatgggtatc gatgtagtaa atataggatt agctactact cctactacag aattagcagt
     4801 aaccatggaa aatgctattg gcggtattat acttactgct agccataatc caacccaatg
     4861 gaatgcacta aaactgctaa acgaaaaggg agaattttta gacgataaag caggtaaagc
     4921 aattttagaa attgctaacg atgaaagctt tcaattcgca gatgtattgg agttaggtaa
     4981 attaactgaa aataatacat tcaatcaaaa acatatcgac agcgttcttt cactcaaact
     5041 agtggatgta gatgctatac gcaatgcaaa atttcgtgtt gccattgact gtgtcaattc
     5101 agttggaggt attataattc ctcaactttt atctgcatta ggggtaacag aaattgtacc
     5161 tttatattgc gaacctaatg gtaagtttcc acacaaccct gaacccttac ccgaacactt
     5221 aactgacata gcttctattc tcacaaacgg aaaagcagat gtaggttttg tagtagaccc
     5281 agacgttgat cgtttagcaa ttatctctga aaatggtgaa atgtttggag aagaatatac
     5341 cttggtttct gtttctgatt atgtattgca acac
//
LOCUS       DAOSMY010000070         5347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15457_length_5347_cov_3.678005, whole genome shotgun sequence.
ACCESSION   DAOSMY010000070 DAOSMY010000000
VERSION     DAOSMY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15457_length_5347_cov_3.678005"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLD76_02605"
     CDS             complement(<1..506)
                     /locus_tag="PLD76_02605"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOU26928.1"
                     /translation="MINVAIFASGSGSNAENIIRYFTGSQLVNFSCILSNKQDAFVHQ
                     RAKKLLIPSYTFTKQQFEDGTVLSFLMDEKINFIILAGFLLKVSDNIITQFPNKIVNI
                     HPALLPKFGGKGMYGDYVHQAVVDAHETQTGITIHFVNNKYDDGEIIFQATCDVLSTD
                     TPDDVAQKV"
     gene            808..1041
                     /locus_tag="PLD76_02610"
     CDS             808..1041
                     /locus_tag="PLD76_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU26929.1"
                     /translation="MSEVAEKVKAIIVEKLGVEASEVVPAASFTNDLGADSLDTVEMI
                     MEFEKEFGISIPDDQAQKIATVGDAISYIEANK"
     gene            1060..2319
                     /gene="fabF"
                     /locus_tag="PLD76_02615"
     CDS             1060..2319
                     /gene="fabF"
                     /locus_tag="PLD76_02615"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU26930.1"
                     /translation="MELKRVVVTGLGAVTPIGNDVPTFWNSLIQGVSGAKPITRFDAS
                     KFKTQFACEVKDFDPNQYFDRKEARKYDLFSMFGIVAAKEAVEDAGIDVEKVDLDQIG
                     VIFGTGIGGIKTFAEEVAGFVQNESIPRFNPFFIPKMIIDILPGHVSIMYGFRGINFS
                     TSSACASSTHALADAFNYIRLGKANIMVAGGSEAAITDVGVGGFNALHAISTRNDSPE
                     TASRPFSLSRDGFVMGEGAGCLIFEELEHALARGAKIYAEVKGAGMTADAHHMTAPHP
                     EGLGAINVMKSALKDADMQPDEIDYINVHGTSTPLGDIAETKAIKDVFGEHAYNLNIS
                     SSKSMTGHLLGATGAVEAIACVLSVQNDIVPPTINFTEGDEDPEIDYKLNFTFNKAQK
                     RPVNAALSNTFGFGGHNGSIIFKKFAK"
     gene            2344..3159
                     /gene="rnc"
                     /locus_tag="PLD76_02620"
     CDS             2344..3159
                     /gene="rnc"
                     /locus_tag="PLD76_02620"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU26931.1"
                     /translation="MIKRIINRIRFISFTKNEPYFVLRNLLGFSPKENSYYQLALLHR
                     STGKRNINGVIIDNERLEFLGDAILSAIISDYLYQKFPLKKEGFLTNLRSKIVQREYL
                     DYLAVQMGLDKLMFSQNKHIVSKNAVHVYGNALEALIGAIYLDQGYKKCQQFLEKQVI
                     EKHMNVNEIVKKEYNFKSKLIEWAQKRKYSIIFVVTNSEVNKEKNITTFYTSVLIENI
                     EVGTGCGLNKKESQQKAARDALHKIKKKETGILINTKYKERIELENHSESKKL"
     gene            3179..4999
                     /locus_tag="PLD76_02625"
     CDS             3179..4999
                     /locus_tag="PLD76_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU26932.1"
                     /translation="MKASNHPIHLAHLVFEKAKQYGERAALHYRNDSTKTWVPMSWNE
                     IAKQVTFISKGLVSIGLEENQNVGIFSQNMCEIIIADFAIMGNHAVSVPLYATSTAPQ
                     IDFIVKDAEIGLLFVGEQYQYDVAMEVKKHSKFLKKIIAIDPNIDLKNDPENMYFFDL
                     LLLGQTVNNDDIVEKRKSKASENDLVNILYTSGTTGVPKGVMLHQYNYSVAIRIHKER
                     LAEVTDKDTSMCFLPITHIFERAWTYLCLERGVQVYVNKRPIEIQQTILEVQPTLMCT
                     VPRYWEKVYAAILEKMETLSPALKKIFTWAIQVGKVYNLEYYRHRKSAPILLRLKYIL
                     AEKLVFNKIKSAAGLTNLHFFPCAGSRLADDINEFIHSLGINLVYGYGLTESTATVSC
                     YYPENKEYIIGSIGKIMPEVDVKISDQGEILLKGSTITAGYYKRPDANAESFEDGWFK
                     TGDAGYIDAANNLYITDRIKDLFKTAGGKYIAPQMLESRLSELKFVDQVAIIGDQRKF
                     VTALIVPTFDTLKIYASNHNIPYQSIEELISHPDIIKFYSDCIKEQMKDLASYEQIKR
                     FTLLPKAFTMEDGLLTNTLKLKRKEVAKVFENIIEKMYID"
     gene            5076..5347
                     /gene="prfB"
                     /locus_tag="PLD76_02630"
                     /pseudo
     CDS             5076..5347
                     /gene="prfB"
                     /locus_tag="PLD76_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT         1971 a          797 c          917 g         1662 t
ORIGIN      
        1 accttttgag caacatcatc tggagtatct gttgacaata cgtcacacgt agcttgaaaa
       61 attatttcac catcatcgta cttattattt acaaaatgta tcgtaattcc agtttgtgtt
      121 tcatgagcat ctaccactgc ttgatgtaca taatctccat acatcccctt gcctccaaat
      181 ttaggtaaca aagcagggtg aatatttaca attttatttg gaaattgtgt aataatatta
      241 tctgatactt tcaataaaaa acctgctagg ataataaaat ttatcttttc atccataaga
      301 aaagacaaca cagttccatc ttcgaactgt tgtttggtaa atgtatagga aggaattaaa
      361 agttttttgg cacgttgatg cacaaatgca tcttgtttat tcgaaagaat acatgaaaaa
      421 ttaactaact gactacctgt aaaataacga attatatttt cagcattact tcctgaaccg
      481 gatgcaaaaa tggcaacatt tatcatataa aaaaggcact tatgttttat actttcaaaa
      541 gaaaacgcaa aggtacaatt aagaatttag atgcacaata ttaatcacaa aagaaaattt
      601 attaacaaca attcttattt tattttaaat atatctaatt gaaatacaaa tatttaataa
      661 taaataaata agaattatag tgcacaaaaa ttaaatacgt gtgcacattt ttttgaagaa
      721 atatcttgta ttagcaaaaa aaagtgtaca tttgcatcgc taaaatttca caaatttaat
      781 ttatttatta actaaaaatt aaaagttatg tctgaagttg cagaaaaagt aaaagctatc
      841 attgtagaga aattaggtgt tgaagcatca gaagtagttc cagcagctag cttcacaaac
      901 gatcttggtg cagattctct tgatacagta gaaatgatca tggaatttga aaaagaattt
      961 ggtatatcta ttccagatga ccaagctcaa aaaattgcta cagtgggtga tgctatctct
     1021 tacattgaag caaacaaata atttaagtta actttattca tggagttaaa aagagttgtt
     1081 gtaacaggtt taggtgcagt tactcctatt ggcaacgatg ttcctacatt ttggaattcg
     1141 ttaatacaag gtgtaagtgg agctaagcct attacgcgtt ttgatgcatc aaagttcaaa
     1201 actcaatttg cgtgtgaagt taaagatttt gatccaaatc aatattttga cagaaaagaa
     1261 gcccgtaaat atgacttatt ctcaatgttt ggcatcgtag cagcaaaaga agcggtagaa
     1321 gatgctggta ttgatgttga gaaagtagac ttagatcaaa ttggcgttat ttttggaaca
     1381 ggaattggtg gtataaaaac atttgccgaa gaagtggctg gttttgttca aaatgagtct
     1441 ataccacgtt tcaatccatt ttttattcca aaaatgatta ttgatatttt acctggtcat
     1501 gtatctatca tgtatggatt tcgtggtatt aatttttcta catcttcggc ttgtgcttca
     1561 agtacacatg ctttggctga tgcctttaat tatatacgtt taggcaaagc taacattatg
     1621 gttgcaggag gctcagaagc tgccataact gatgttggtg taggaggttt taatgcttta
     1681 catgccattt ccacacgtaa tgatagtcca gaaacagctt cacgtccttt tagtttatct
     1741 agagacggtt ttgtgatggg tgaaggagcc ggttgtttga tttttgaaga gttagaacat
     1801 gctttagcac gaggtgctaa aatatatgca gaagtaaagg gtgctggtat gacagcagat
     1861 gctcatcata tgacagctcc acatccagaa ggactgggag ctataaatgt aatgaaatct
     1921 gcactaaaag atgcagacat gcaaccagat gaaattgatt atatcaatgt tcatggtacc
     1981 tctaccccat taggtgatat tgctgaaaca aaagcaatta aagatgtatt tggcgaacat
     2041 gcatacaatt tgaatattag ttcgtcaaaa tcaatgacag gacatttatt aggagctacc
     2101 ggagctgtag aagctatcgc atgcgtactt agtgtacaaa atgacatagt tcctcctacc
     2161 atcaatttta cagaaggcga tgaagatcct gaaatagatt ataaattgaa ctttactttt
     2221 aataaagccc aaaaaagacc tgtaaatgca gctttatcga atacttttgg atttggtggg
     2281 cataatggaa gtatcatctt taaaaaattt gcgaagtaat cactttttta acgtgtacta
     2341 gcattgataa aacgaattat taatagaata aggttcattt ctttcacaaa gaacgaacct
     2401 tattttgttt tacgtaacct acttggattt agtcccaaag aaaatagtta ttaccaacta
     2461 gctctattac atcgttcaac agggaaacgc aatataaacg gcgttattat agacaatgaa
     2521 cgattggaat ttttagggga tgctatttta tctgctataa tctctgatta tttgtatcaa
     2581 aaatttccac ttaaaaaaga aggattttta actaatctac gttcaaaaat tgttcaaaga
     2641 gaatatttag actatctagc tgtccaaatg ggtttagata aactaatgtt ttcccaaaat
     2701 aaacatattg tctctaaaaa tgcagttcat gtatatggaa atgctcttga agcattaatt
     2761 ggtgcaattt acctagatca aggatataaa aaatgtcaac aatttttaga gaaacaagtt
     2821 attgagaaac acatgaatgt gaacgaaatt gtaaaaaaag agtacaattt taaatcaaaa
     2881 ctaatagaat gggctcaaaa aagaaaatat tccattatat tcgtagttac aaatagcgaa
     2941 gtaaataaag aaaaaaatat aaccacattt tatactagtg tattaattga aaatatagaa
     3001 gttggtacag gttgtggttt aaacaaaaaa gagtctcaac aaaaggcagc aagagatgct
     3061 ttacacaaaa taaagaaaaa agaaactggg attcttatca atactaaata taaagagcgt
     3121 atagaactag aaaatcactc tgaatcaaaa aaactataga actaaatcat tattatatat
     3181 gaaagcaagt aatcacccta tccatttggc tcatttagta tttgaaaaag caaagcaata
     3241 tggagaaaga gcagcgttgc actatcgcaa tgacagtaca aaaacctggg ttcctatgtc
     3301 atggaatgaa atagcaaaac aagtaacttt tattagtaaa ggattagttt ccattggctt
     3361 agaggaaaat caaaatgttg gtattttttc acaaaatatg tgcgaaatta tcattgcaga
     3421 ttttgcaatc atgggtaacc atgcagtatc agtaccttta tatgctactt ctactgctcc
     3481 ccaaatagat ttcatcgtaa aagatgctga aattggtcta ttgtttgtag gcgaacaata
     3541 ccaatatgat gtagcgatgg aagtaaaaaa gcactcaaaa tttttaaaga aaattattgc
     3601 cattgatcca aatattgatt taaaaaacga tcccgaaaat atgtactttt ttgatttact
     3661 tttacttgga caaactgtta acaatgatga tatagttgag aaaagaaaat caaaagcatc
     3721 tgaaaatgat cttgttaata tattatacac ttctggtaca actggcgttc caaagggtgt
     3781 tatgttacat caatataatt attctgtagc tattcgcatt cacaaagaaa gattggctga
     3841 ggttactgat aaagatactt cgatgtgttt cttgcccatc acacacatat ttgagcgagc
     3901 atggacttat ttgtgtttag aacgaggagt tcaagtatat gtaaataaac gtcccataga
     3961 aatacagcaa acaatactcg aagtacaacc tacattgatg tgtacagtgc ctcgctattg
     4021 ggaaaaggtt tatgctgcca ttctcgaaaa aatggaaaca ctttctccag ctttgaaaaa
     4081 aatatttaca tgggctattc aagtgggcaa ggtgtataat ttagagtatt accgccatcg
     4141 caaatctgca ccaatcttac tacgattaaa atatatcttg gcagaaaaac tggtatttaa
     4201 taaaataaaa agtgcagctg gtcttactaa tttacatttc tttccttgtg caggatctcg
     4261 tttagctgat gatattaacg agtttataca tagcctcgga attaatttgg tatatgggta
     4321 tggtttaaca gaatcaactg ctaccgtttc atgttattat cctgaaaata aagaatatat
     4381 aataggatct attggtaaaa ttatgcctga agtagatgtt aaaatttcag atcaaggcga
     4441 aattttactc aagggaagta ccataaccgc aggttattat aaacgtcctg atgctaatgc
     4501 agaatctttt gaagatggct ggttcaaaac aggagatgca ggctatattg atgctgctaa
     4561 taatttatat attactgata gaataaaaga cctatttaaa actgcaggag gtaaatacat
     4621 tgctccacaa atgctcgaaa gccgattatc tgaattaaaa tttgttgacc aagttgctat
     4681 tattggcgat caacgcaaat ttgttacagc ccttattgtg ccaacatttg atacattaaa
     4741 aatctacgct tctaatcata atatacccta tcaatcaata gaagagctta tttctcatcc
     4801 tgatattatt aaattttata gcgattgtat taaagaacaa atgaaagatt tggcaagcta
     4861 cgaacaaata aaaaggttta ccctacttcc aaaggcattt acgatggaag acggattact
     4921 tacaaatacc ttaaaattaa aacgaaaaga agtagcaaaa gtattcgaaa atataattga
     4981 aaaaatgtat attgattaaa aaatgatact agaaaacaga ctccacatca caataaaatt
     5041 gtatctttgt gggtttgaaa aaatattcaa taaatatgat aacaatagaa caactaaaag
     5101 agacgataga gcgccaacaa gcgttgagga ggtatctttg acattgatgc aaaattagta
     5161 gccttacaag aagaggaatt acgaactcaa gaccctttgt tttggaatga cagcaaaagt
     5221 gctgaaattc aaatgaaaaa gataaaagaa ttaaagtttt ggatagaaac atataataaa
     5281 gtttaccaag ctactgaaga gctgaaactt gcatttgatt tctacaaaga agctatcgtc
     5341 gctgaac
//
LOCUS       DAOSMY010000071         5340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15489_length_5340_cov_3.410596, whole genome shotgun sequence.
ACCESSION   DAOSMY010000071 DAOSMY010000000
VERSION     DAOSMY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15489_length_5340_cov_3.410596"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLD76_02635"
     CDS             complement(<1..743)
                     /locus_tag="PLD76_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU26933.1"
                     /translation="MSTKLNKVVKELNVGLATAVEFLHKKGFADVESNPNARITEEQY
                     DLLVKEFSKDKNQKIESGILAQRSKERPKGEVVALEGFDKENKPVEEIKVEIQETSRP
                     QFKQVGKIDLDALVPKKKDANEVKEEMPNIDVPLQENVEKIVTVQQVEQAPIQEVEEE
                     ITEPSVVVEAEIEDESETENVVEDIDESVDDTISAELSDDVYTIEKNTIHSTINVVGK
                     IDLDALNQQTRPKKKTKEEKKKEGLEREI"
     gene            complement(828..2081)
                     /gene="nusA"
                     /locus_tag="PLD76_02640"
     CDS             complement(828..2081)
                     /gene="nusA"
                     /locus_tag="PLD76_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU26934.1"
                     /translation="MSNKPVQSLNLIETFSEFKELKNIDKATMISVLEDSFRSVIAKM
                     FGTDESFTVVINPDKGDCEIWRDYEVVADGEVEDEVLQMGLSKARAIDDECEIGDDVT
                     EAVNFLSFGRRAVLTLRQTLASKILELQKESVYDQYKEMVGQVVYGEVYQIWKKEILL
                     LDDQGNELLLPKTEQIPTDFYKKGDTVKSVVVRVDNKNNQLKIIVSRTAPIFLQRLFE
                     QEIPEVQEGTIMIRRIARIPGERAKVAVESYDDRIDPVGSCVGVKGSRIHSIVRELRN
                     ENIDVVNYTSNLSLFIARSLSPAKVLSIRIDEERKRAEVSIKPEDVSMAIGKGGSNIK
                     LATMLTEYTIEVFRHVDEEDEDDVFLDAFSDEIEPWVIDVFKSIGCDTAKSVLEIPRQ
                     ELIDRTDLEEETIDEALAILRQEFE"
     gene            complement(2087..2557)
                     /gene="rimP"
                     /locus_tag="PLD76_02645"
     CDS             complement(2087..2557)
                     /gene="rimP"
                     /locus_tag="PLD76_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOU26935.1"
                     /translation="MISKDVVVQAVNQFLKDKEDYFLVDVTISIHNTICVEIDAFQGV
                     DVETCCELSRFIETKIDREIEDFELEVGSAGITAPFKILQQYIKNINNEVEVLLKNGK
                     KIIGFLRSANEHTFVVEVSQKIKKDKNAKKEEVTELLTYAYSDVKHTKYNLRFK"
     gene            2705..3427
                     /locus_tag="PLD76_02650"
     CDS             2705..3427
                     /locus_tag="PLD76_02650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOU26936.1"
                     /translation="MTQTYIHKDLGKILIYYSPKARSIRVQVGDNGIKLIIPLGVNID
                     KGLHFVNAKQDWIEKALKRTEQKKRHVSIHITELNSQTFSILTKACERTNILFRLHEK
                     ILTIEHPISLDITQAKYQQILLDGMEKFVVSEAKKHFSQRLAYLANKGGFTYNEFRIQ
                     RSKTRWGSCSSKKNINLCAYLLFVPAYLSDYVIIHELCHTKEMNHGNTFWNLVASQEP
                     NYITYRKEIKQYSFLMQLFNRK"
     gene            3455..4780
                     /locus_tag="PLD76_02655"
     CDS             3455..4780
                     /locus_tag="PLD76_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOU26937.1"
                     /translation="MQWETLLSTKRFGMEAYHKKDTEDRSEFQRDYDRLIFSSPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVACVGRSLGTNIAHQLSILHQKSSNFLPEIGSIV
                     SAACLAHDLGNPPFGHSGEKAIASFFSEGNGSFIKERLENETEWNDLIFFEGNANSLR
                     LLTHQFKGRRYGGFAMTYSTLASIVKYPYSSVECGKKGKFGFFESEKATYEQIAMELG
                     IIKSPVSDKKYARHPLVYIVEAADDICYQIMDIEDAHKLKILSTEQTKELLLQFFDTE
                     RKKRIYETWKLVDDSNEQISYLRSSVIGKLVSECARVFVENEQQILSGQFSGSLIDHI
                     EPVCKNAYENCSTIAVKKIYKSQEVLDIEVAGYKIIQTLLDEYIKASFQPNKSYSKLL
                     LDRVPSQYDMYAPSTYEKIQAIVDFISGMTDIYALDLYRKITGMNLPSL"
     gene            4857..>5340
                     /locus_tag="PLD76_02660"
     CDS             4857..>5340
                     /locus_tag="PLD76_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOU26938.1"
                     /translation="MIVGVGGVGAYAAEMICRAGIGNITIIDADTIKESNINRQLPAL
                     HSTIGKKKVTVLTERLLDINPNLVLKQHDVFIEKELIPSLFENLHVDFVIDAIDTLSP
                     KIDLIRYCIQHKIKIVSSMGAGGRTDPSKIHYADISGTYHCALAKHVRKRLKLVNISK
                     G"
BASE COUNT         1703 a          983 c          799 g         1855 t
ORIGIN      
        1 tgaatttcgc gttctaaccc ttcttttttc ttttcctctt ttgttttctt ttttggacgt
       61 gtttgttgat ttaacgcatc taaatcaatt tttccaacca cattgattgt ggaatgaatg
      121 gtgtttttct ctatcgtata tacatcatca cttaattcag cagatatagt atcatcaacc
      181 gattcatcta tatcctctac tacattttcc gtttccgatt catcctctat ttcagcttct
      241 actaccacag aaggttctgt tatttcttct tccacttcct gtataggagc ttgttctact
      301 tgctggactg ttactatttt ttcgacattt tcttgcaaag gaacatctat attaggcatt
      361 tcttctttaa cctcattcgc atctttcttt ttaggcacaa gagcatctaa atcaatcttg
      421 ccgacttgtt taaattgagg acgagatgtt tcttgaattt ccaccttaat ttcttctact
      481 ggtttgtttt ctttatcaaa accttcgaga gcaacaactt ccccttttgg tcgttcttta
      541 cttcgttggg cgagaatacc agattcaatt ttttggtttt tatctttgct aaattctttg
      601 acgagcaaat catattgctc ttcggttata cgagcattgg gattagattc cacatccgca
      661 aagccttttt tatgtaaaaa ctctacagcc gtagctaatc ctacatttaa ttcttttact
      721 actttattta attttgtcga catataaata ctccttgttt tcttctaatt aaattttctt
      781 tttgacaatg ctcatctttt cctaatcctt ttgcaaaaaa tgacctatta ttcaaattct
      841 tgccttagta ttgccaatgc ttcatctatt gtttcttctt ctaaatcagt tctatcaatc
      901 aattcttgac gaggaatttc taatacgctt tttgctgtat cacatccgat agatttaaaa
      961 acatctataa cccatggttc aatttcgtca gagaatgcat ctaagaacac atcatcttca
     1021 tcttcctcat ccacatgtct gaatacttcg atagtgtatt ctgtaagcat tgtagctaat
     1081 tttatattgg atcctccttt gccaatagcc atagacacat cttctggttt aattgacact
     1141 tctgcacgtt ttctttcttc gtctatgcga atcgaaagca cttttgcagg acttaatgaa
     1201 cgtgcaataa ataatgataa attagatgta taattaacaa catcaatatt ttcatttctc
     1261 aattccctaa caatgctgtg gatacgagaa ccttttactc ctacgcaaga accaacaggg
     1321 tcaattctat catcatatga ttctacggct acttttgctc tttcgccagg tatacgagcg
     1381 attcggcgta tcataatagt accttcttgc acttcaggta tttcctgttc aaataaacgt
     1441 tgtaaaaata ttggagctgt acgagacact ataattttca gctgattatt tttattatca
     1501 actctcacta caacagactt taccgtatct ccttttttat aaaaatcagt aggtatttgt
     1561 tctgtttttg gtaataataa ctcgttgcct tgatcatcta acaacaagat ttcttttttc
     1621 caaatttggt acacttcgcc ataaacaact tgaccaacca tttctttgta ttgatcatat
     1681 acagattctt tttgtaattc taagattttt gaagccaatg tttgacgcaa agttaataca
     1741 gcacgtcttc cgaaactcaa aaagttaact gcctctgtaa catcatcacc tatttcgcat
     1801 tcgtcatcaa tggcacgagc ttttgacaag cccatttgta aaacctcatc ttctacttct
     1861 ccatcggcta ctacttcata gtctcgccaa atttcacaat cacctttatc aggattaatt
     1921 acgactgtaa aactctcatc ggttccaaac attttcgcta ttacactacg aaaagaatct
     1981 tcaagcacac taatcatcgt ggctttatca atatttttta attctttaaa ctctgaaaat
     2041 gtttctatca aattcagtga ttgtactggt ttgttgctca taattgttat ttaaatctga
     2101 gattatattt tgtatgttta acgtctgaat acgcataagt tagtaactcc gttacttctt
     2161 cttttttggc atttttatct tttttgattt tttgcgacac ttcaaccaca aatgtatgct
     2221 cattggcact tcgcaaaaat ccaattattt ttttcccgtt tttcaataaa acttcaactt
     2281 cgttgttgat atttttgata tattgctgaa gtattttaaa aggtgctgtt atcccagcag
     2341 aacctacttc taattcaaaa tcttctatct ctcggtcaat ttttgtttca ataaaacgac
     2401 tgagttcaca gcatgtttct acatctacac cttgaaaagc atctatttcg acacaaatag
     2461 tgttatgtat gcttattgta acatccacta agaaataatc ttctttatct ttcaaaaatt
     2521 gattaacagc ctgaacaaca acgtctttag atatcatacc ttactttatg tgaacaaatg
     2581 aggggaacta ccgcccccct cacctatctc tttttcggtt gcaaatatac gaataattct
     2641 tttactgcca aaatattagc ttttttttct acatttgcat gtgaaagtac ttaagctcta
     2701 aaacatgaca caaacttata tacacaaaga cttaggcaaa attttgattt actatagccc
     2761 aaaggctcgt tctatacgtg ttcaggtggg tgataatgga ataaaattaa ttattccttt
     2821 gggtgtaaat atagataaag ggctacattt tgtcaacgcc aaacaagatt ggattgaaaa
     2881 agccttgaag cgaaccgaac aaaaaaagag acatgtatct attcatatta cggaattaaa
     2941 ttcacagaca ttttctatct taacaaaggc ttgtgaaaga acaaatatat tatttcgttt
     3001 gcacgaaaaa attttaacga ttgaacatcc aatttctttg gatattacac aagctaaata
     3061 ccaacagata ttacttgatg gaatggaaaa atttgtagtt tcagaagcaa agaaacattt
     3121 ttcgcaacga ttagcttatt tagcaaataa gggaggattt acgtataacg aatttcgcat
     3181 tcaacgttca aaaacacgtt ggggtagttg ttcctccaaa aaaaatatca atttatgtgc
     3241 gtatttactt tttgtaccag catatcttag cgactatgtt atcattcatg aactttgtca
     3301 tacaaaagaa atgaatcacg gaaatacatt ttggaattta gtggcaagcc aagaacctaa
     3361 ttatattact tatcgaaaag agataaaaca atattcgttt ttaatgcaat tattcaacag
     3421 aaaatagaac attatacata tgaaacaatc aacaatgcaa tgggaaactc ttttatctac
     3481 caaacgtttt gggatggaag cgtatcataa aaaagacaca gaagatcggt ctgaatttca
     3541 gagggattat gatagactca tcttttcatc tccttttagg cgtttacaaa ataaaacaca
     3601 agttttccct cttcctggta gcgtatttgt tcataatagg ttaacacata gcttggaagt
     3661 tgcctgtgtt ggtcggtcat taggcactaa tattgctcac caattaagca ttttacatca
     3721 gaaaagctcc aattttttac ccgaaatagg atcgatagta tcagcagctt gtttagcaca
     3781 tgatttgggc aatcctcctt ttggacactc tggcgaaaaa gctattgcta gttttttttc
     3841 cgaaggaaat ggttctttta taaaagaaag gcttgaaaat gaaactgaat ggaatgattt
     3901 aatttttttt gaaggaaatg caaattctct tcgattgtta acgcatcaat ttaaaggtag
     3961 gcgatatggt ggttttgcaa tgacttattc cacattagct tcgatcgtaa aataccccta
     4021 ttcatctgtc gaatgtggca aaaaagggaa atttggtttt tttgaatcag agaaagcaac
     4081 ctatgagcaa attgctatgg aactaggtat aatcaaaagc ccagtttctg ataaaaaata
     4141 tgcacgacat cctttagttt atatagtaga agctgctgac gatatctgtt atcagataat
     4201 ggatatagaa gatgcacata agctaaagat actatcaaca gagcaaacaa aagaattatt
     4261 attgcaattt ttcgatacag aacgaaaaaa acgcatctat gaaacatgga aattagttga
     4321 cgactcaaac gaacaaattt cttatcttcg ctcttctgtg attggaaaat tagtatctga
     4381 gtgtgcacgt gtatttgtgg aaaatgagca acaaatactt tctggtcaat tttctggatc
     4441 attaatagat cacattgaac cagtctgtaa aaatgcttat gaaaactgct ctacaatagc
     4501 tgtcaaaaaa atatacaaat cacaagaagt attagatata gaagtagctg gctataaaat
     4561 tattcaaact ttacttgatg aatacataaa agcaagtttt caaccaaata agtcctattc
     4621 aaaattatta ttggatagag ttccaagcca atacgacatg tacgctccaa gtacttacga
     4681 aaaaattcaa gctattgtag attttatatc gggtatgaca gatatttacg cattagactt
     4741 gtaccgtaaa attacaggca tgaatttacc ttcattataa taatagtatg tggactagta
     4801 gaactaaatt attactgggg gagaacaata gtagtatcct tgagaaaaaa cacgttatga
     4861 tagtaggtgt tggaggtgta ggtgcatatg cagccgaaat gatttgtcgt gcaggcatag
     4921 gcaacatcac tattattgat gcagacacga taaaagaaag caatattaac aggcaacttc
     4981 ctgctttaca tagcacaata ggcaaaaaaa aagtaaccgt attaacggaa cgcctccttg
     5041 acatcaatcc taatttagta cttaaacaac atgatgtatt tattgagaaa gaacttatac
     5101 cctctctgtt tgaaaatctt catgtagatt ttgtgattga tgctattgat acactatccc
     5161 ctaaaattga cctcattcgt tattgtattc aacataaaat aaagattgtt tcctcaatgg
     5221 gtgctggagg cagaacagat ccaagcaaaa tacattatgc tgacatttca ggtacatacc
     5281 attgtgctct tgcaaaacat gtaagaaaaa gattaaagtt ggtaaatatc tcaaaaggat
//
LOCUS       DAOSMY010000072         5316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15584_length_5316_cov_3.691884, whole genome shotgun sequence.
ACCESSION   DAOSMY010000072 DAOSMY010000000
VERSION     DAOSMY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15584_length_5316_cov_3.691884"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..443)
                     /locus_tag="PLD76_02665"
     CDS             complement(57..443)
                     /locus_tag="PLD76_02665"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOU26939.1"
                     /translation="MTKEKISHEYNLRTTISLTHMWQYFHTEDGLGTWFADKVISKNG
                     IYEFFWENTSQKAKILHKTQQFSVRFHWIDDEEETTYFQFKLERDDITNVVTLTITDF
                     SYPDEREDTIKLWNTQLEKLRRTIGL"
     gene            complement(456..941)
                     /locus_tag="PLD76_02670"
     CDS             complement(456..941)
                     /locus_tag="PLD76_02670"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU26940.1"
                     /translation="MKKISLHTAIEVYTYEELTDQQKTLIQKAKETSLSAYSPYSKFS
                     VGAAILLENGEIVCGSNQENAAYPSGICAERTAMFYANSTYPTVAPICIAIVAFTDGK
                     FKQTAISPCGACRQVLIESEIRFNKPITMLLYGEPEIFFINSITDLLPLNFAPSSLLS
                     S"
     gene            1177..2130
                     /locus_tag="PLD76_02675"
     CDS             1177..2130
                     /locus_tag="PLD76_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOU26941.1"
                     /translation="MKLIRFTFLFFVCIVPTIALFSQQYRNPKYQTYIEKFSPIAIEN
                     MQKHGIPASITLAQGILESGIGDSRLAREANNHFGIKCHDWKGEYILHTDDKVNECFR
                     KYKNPEESYEDHSVFLTTRGRYAHLFELKITDYKAWAFGLQRAGYATDPTYATRLINL
                     IEENGLHTFDKQLLQPSKQKSVAKEETKHDTEKPSREHKHDRYPSRPTQRNMYGLRYV
                     IASSNDTYTSIAKEFKVSLRSILKWNDLPYEVSLKEGEIVYLQSKKKRVSSYSTVHYV
                     ASGETMHIISQRYGIRLKSLYDLNNMNYSQVPTIGQKLYLR"
     gene            2131..2811
                     /locus_tag="PLD76_02680"
     CDS             2131..2811
                     /locus_tag="PLD76_02680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU26942.1"
                     /translation="MPIFKENMDIHKRLLYHAFVFPFLFLFIFWLVFLVERVLYTDFT
                     VFGIFPLATKGLLGIITMPFIHADSTHLLANSVTFLVLSVLLFSNYRHIALKVFILIW
                     LFSGVILWVIGRPSWHIGASGVIYGMAFFLFCSGLLRKKIPLVATSLVVVFLYGGMVW
                     YMFPLGIDNHISWEGHLSGALIGMFFSVYYKIKEPSEQKQEDIDENDSVDYDWQENEI
                     ENTTEDTI"
     gene            2873..3838
                     /locus_tag="PLD76_02685"
     CDS             2873..3838
                     /locus_tag="PLD76_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26943.1"
                     /translation="MEPEKELDLIDLLKLLGNSIGKGISAISRFIMWFIKFCVKNWYI
                     LLVSVAFGIACAFYFSQSKFSKYEGIIVVENNVESTPEFYTEVQSLASYMNNDNTSFS
                     MNNDNTSFSSKFGVSTEIGKKIFKLEPLYIYTSENEGLYNFIDEKKKYSDLEPHQQKF
                     AILIKTNDRSLFPFLKTALYKYFENHPYFKGRNVARLNSLDVQQRTLQNEIATLDSLK
                     KLEYFEANSKTKNLKMDNALLIGESNTQLYHTNILEMMEKLQRTKIDIETRNSVVTIS
                     SDFQIKSPQNARVKSLVLFGSAFFVIGFCLALFIRHRNKIKEFLK"
     gene            complement(3923..4378)
                     /locus_tag="PLD76_02690"
     CDS             complement(3923..4378)
                     /locus_tag="PLD76_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26944.1"
                     /translation="MKKTILIVALLVGFVASGVAQSRSIGLRSFQEVSYQHGFGSSNM
                     LEIDGGFFNTGLQATATYNWLFPIDWDYEGSWNWYVGPGAYAGIRSNSNNNDGLFIGA
                     AGMIGIEYNFWFPLQLSIDYRPTIALFNSNNTPFGFYSNTVGLGVRYIF"
     gene            complement(4476..>5316)
                     /locus_tag="PLD76_02695"
     CDS             complement(4476..>5316)
                     /locus_tag="PLD76_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU26945.1"
                     /translation="FPQQDGYDITLALYDLEGNLLKKQSDNKGFTGEWNATYTGWVTL
                     KVWNNNSENISQKCWVRVKYYAPPTITDVYTDTPDTRVSIWTANGNTSTWNDCNNWEQ
                     GKVPANNATVIIPNHTPIVPIMSGNLTLKKLVIEQGKGNIGSPDLIVNGSLTVNELTI
                     LSGTAYICGNGTTEVSIQNGSVSNCATRIEEPSFSIEHISVIPNPVESICELRFISNS
                     IGVAYFTLTDVTGKTILTKEIPINAIGINSTNLYLGGIHTGTYIAKINCNNRISSVMI
                     VKK"
BASE COUNT         1737 a          798 c          888 g         1893 t
ORIGIN      
        1 tagtatttaa agtgttaaca aaacgtattc tgttgttatt tttgaatcaa atgtttttat
       61 aaaccaatcg ttctacgaag tttttccagt tgggtattcc atagttttat tgtatcttct
      121 cgttcatctg gatatgaaaa atcagtgata gtaagtgtta caacattagt tatatcatca
      181 cgttctaatt taaattgaaa ataagtagtt tcctcttcat catctatcca atggaatcga
      241 acagaaaatt gttgtgtttt atgcagtatt tttgcttttt gggaagtgtt ttcccaaaaa
      301 aattcgtata tcccattttt actaattact ttgtctgcaa accaagtgcc caaaccatct
      361 tcagtatgaa aatattgcca catatgtgtt agcgaaatgg ttgtacgaag gttatattcg
      421 tgagagattt tttcttttgt cataagcttt tggtattatg aagataacaa actagaagga
      481 gcaaaattga gaggcaataa gtctgttatg ctgttaatga aaaaaatttc aggttctcca
      541 tatagcagca tagttatagg tttattaaaa cgaatttcag attctattag tacttgtctg
      601 catgctccac atggagatat agctgtttgt ttaaattttc cgtcagtaaa agctacaata
      661 gcaatacaaa tgggagcaac cgttggataa gtagaatttg cataaaacat agctgttcgt
      721 tcagcacata tgcctgaggg atacgctgca ttttcttgat tagagccaca aactatttcc
      781 ccattttcta gtaaaatagc agcaccaaca ctaaattttg aatatgggct ataggcagat
      841 aaagaggttt ctttggcttt ttgtatgagt gttttttgtt ggtcggtaag ctcttcgtat
      901 gtatatacct caattgccgt atgtaaactt atttttttca ttatgttaca attaatgttt
      961 tagtacagat ttatttagta tttgcttcgt tagataaatt ttctatcaat tagaaacttt
     1021 tatatcatac tatccatatt ttacaaacat aactaaaatt cactaaaaaa acaatttttt
     1081 tagcattttt cgtaactaaa atactatttt tgtatctatt attttatcaa cgattattaa
     1141 tgggtttata attttaaata gtgagagttc ctaagcatga aattaatacg atttaccttt
     1201 cttttttttg tatgtatcgt tccaacgata gctctttttt ctcagcaata tagaaatccc
     1261 aaataccaaa cttatatcga aaaatttagc cctattgcta tagaaaatat gcagaaacac
     1321 ggaattcctg caagtattac attggctcaa gggattttgg agtcaggtat cggggatagt
     1381 cgtttggctc gtgaagccaa caatcatttt ggtatcaaat gtcatgattg gaaaggggaa
     1441 tatattttac atactgatga taaagtaaat gaatgtttta gaaaatataa aaatcctgaa
     1501 gaatcgtacg aagatcattc ggtattttta accacaagag gtcgctatgc tcatttattt
     1561 gagttgaaaa ttactgatta taaggcttgg gcctttggtt tacaacgtgc aggttatgca
     1621 acagatccaa cttatgccac cagattaatc aacttaattg aagaaaatgg attgcataca
     1681 tttgataaac agcttctaca gccttctaag caaaaatcag ttgctaagga agaaacgaaa
     1741 cacgacactg aaaagccatc acgagaacac aagcatgata gatatccatc acgaccaaca
     1801 caacggaata tgtacggatt acgttatgtg attgcctctt ctaatgatac gtatacatct
     1861 atagctaagg aatttaaggt atctttacgt tctatactca aatggaatga tttgccctat
     1921 gaggtaagtt tgaaagaagg agaaattgtt tatttgcaaa gcaagaaaaa acgcgtgtcg
     1981 agttatagca cagtacatta tgttgcctca ggagaaacga tgcatataat ttctcaacgt
     2041 tatggaatac gattaaaatc gttatatgat ttgaataata tgaattattc tcaggtacca
     2101 actattggtc aaaaattata cctaagatag atgcctattt ttaaagaaaa tatggatatt
     2161 cataaacgtt tactttatca tgcgtttgtt tttccttttt tatttctttt tattttttgg
     2221 cttgtatttt tggtagagag ggtactctat actgatttta ctgtatttgg catatttcct
     2281 ctagcaacta aagggttatt gggaattata acaatgccat ttattcatgc agatagcaca
     2341 catctcctag ctaattccgt cacttttttg gtcttatctg ttttgttgtt ttctaattat
     2401 aggcatattg cactaaaagt atttattctt atctggcttt tttcaggcgt aatattatgg
     2461 gttattggta ggccaagttg gcatataggg gcaagtggag taatttatgg aatggctttt
     2521 tttctttttt gtagtggatt gcttcgaaaa aaaataccct tagttgctac ttctttggtt
     2581 gtagtttttc tatatggagg tatggtgtgg tatatgtttc ctttaggtat tgataatcat
     2641 atttcgtggg aagggcattt gtcaggagcg ttgataggta tgtttttttc tgtttattat
     2701 aaaatcaaag agccatctga acaaaaacaa gaggatatag atgaaaatga tagtgttgat
     2761 tatgattggc aagagaacga gatagaaaat acaacagaag atactattta aatcaatatt
     2821 ttatgtattt ttgtgcatca aaagagttac aaatataaac aacagaaaca gtatggaacc
     2881 agaaaaggaa ttagatttaa ttgatttatt aaaattgtta ggtaattcga taggtaaagg
     2941 aatatctgct attagtagat ttattatgtg gtttatcaag ttttgtgtaa aaaattggta
     3001 tatactactt gtttccgtag cttttggaat agcttgtgct ttttattttt cacaatctaa
     3061 attttcaaaa tatgaaggaa taattgtagt agagaataat gtagaaagta cccctgagtt
     3121 ttatacagaa gtacagtctt tggcatctta tatgaacaat gataatacat ctttctcgat
     3181 gaacaatgat aatacatctt tctcgtctaa atttggtgtt tcaactgaaa ttgggaaaaa
     3241 gatatttaag ttggaacctc tttatattta taccagcgaa aatgaaggat tgtataattt
     3301 tattgacgaa aaaaagaagt attcagattt ggagcctcat caacaaaaat ttgctatttt
     3361 aataaagaca aatgatcgtt cgttattccc gttcttaaag acagcattgt acaaatattt
     3421 tgagaatcat ccctatttta aagggcggaa tgtggctcgg ttaaattctt tagatgtgca
     3481 acaacgcaca ttgcaaaatg aaatagcgac gttggatagt ttgaaaaaat tggaatattt
     3541 cgaggctaat tctaaaacaa aaaatttaaa aatggataat gctttattaa taggagagtc
     3601 caatacgcag ttgtatcata ctaatatttt agaaatgatg gaaaaattgc agcgtacgaa
     3661 aattgatatt gaaacaagaa atagcgtagt aacgattagt tctgattttc agataaaatc
     3721 tccccaaaat gctagagtaa agtctttagt tttatttggt tctgctttct ttgttattgg
     3781 tttttgtctc gctctattta ttcgccatag gaacaaaata aaggagtttt taaaataaca
     3841 cgttcatcag ttataaaaca atagcgagtt caactttttg aactcgctat tgttttataa
     3901 agactataca cttgtaattt tgttagaaaa tatacctaac tcctaatcca acagtatttg
     3961 agtagaatcc aaaaggcgta ttattactat taaataaagc aattgttggg cgataatcta
     4021 ttgataattg aagaggaaac caaaagttat attcgatacc aatcatacct gctgctccaa
     4081 taaataatcc atcattgtta ttactgttcg agcgaatacc tgcatatgca cctggaccta
     4141 cataccaatt ccaactgcct tcataatccc aatcaattgg aaacaaccaa ttgtatgtag
     4201 cggttgcttg taaacctgta ttaaagaatc ctccgtcaat ttctagcata ttgctactac
     4261 caaatccatg ttgataactt acttcttgga aagatcgtaa tcctattgaa cgtgattgtg
     4321 ctacaccact tgctacaaag cctactaata aggctacaat taaaatcgtt tttttcataa
     4381 tttattcgtt ttagttaaaa aataaacctt tactttttcg cgtgcaaagt aaaggtttat
     4441 tttctgaaaa agcaattaaa attagttgtt attccttatt ttttaacaat cattacagaa
     4501 gaaattcggt tgttacagtt tatttttgca atatatgttc cagtatgtat acctcctaaa
     4561 tataagttgg tagaattgat cccaatagca ttaataggga tttcttttgt cagaattgtt
     4621 tttccagtta catcagttaa tgtgaaataa gctacaccga tactattgga aataaaacgc
     4681 aattcgcaga tagattctac tggattgggt atgactgata tgtgttcaat actaaaagaa
     4741 ggttcttcaa tacgtgttgc acaatttgat acagaaccat tttgaataga tacctctgtt
     4801 gtaccattgc cacaaatata tgctgtacca gataaaatag tcaattcgtt tactgttaaa
     4861 gaaccattaa ctatcaaatc aggacttcca atattgcctt taccttgctc aataaccaat
     4921 ttttttaatg taagatttcc agacataatg ggcacaattg gggtgtgatt aggaatgata
     4981 accgtagcat tattagctgg aactttgcct tgttcccaat tattacaatc gttccatgtg
     5041 ctagtattac cgtttgctgt ccaaatactt actcgtgtat caggggtatc tgtatacaca
     5101 tccgtaatag tgggcggagc atagtattta actcttaccc aacatttttg tgagatattt
     5161 tcagaattat tattccacac tttcagtgtt acccaccctg tataagttgc attccattct
     5221 ccagtaaatc ctttgttatc actttgtttc tttaagagat ttccttctaa gtcatacaat
     5281 gccaatgtta tatcatatcc atcttgttgt gggaaa
//
LOCUS       DAOSMY010000073         5293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15681_length_5293_cov_3.255250, whole genome shotgun sequence.
ACCESSION   DAOSMY010000073 DAOSMY010000000
VERSION     DAOSMY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15681_length_5293_cov_3.255250"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLD76_02700"
     CDS             <1..315
                     /locus_tag="PLD76_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26946.1"
                     /translation="LEKIFDAKQGTPESDEADILGMLIDEYEKKHYPIEAPDPIEAIK
                     IRMEEMQLKQADMVEIIGGKSRVSEVLNRKRRLTVNMIRNITKRLNISPNVLINDYKL
                     SR"
     gene            complement(831..905)
                     /locus_tag="PLD76_02705"
     tRNA            complement(831..905)
                     /locus_tag="PLD76_02705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(868..870),aa:Pro,seq:tgg)
     gene            1083..2840
                     /gene="aspS"
                     /locus_tag="PLD76_02710"
     CDS             1083..2840
                     /gene="aspS"
                     /locus_tag="PLD76_02710"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU26947.1"
                     /translation="MYRTHTCGELRLSHIGQQVTLAGWVQRVRRMGGMIFVDLRDRFG
                     ITQLVFNQETNADLCAQANELGREFVIQVQADVAERSNKNKHIPTGEIELLVSTMRVL
                     NTSETPPFTIEDNTDGGDDLRMKYRYLDLRRNTVRENMILRHKVAFETRRYLDQMGFL
                     EVETPILIASTPEGARDFVVPSRMNQGQFYALPQSPQLFKQLLMVSGFDKYFQIAKCF
                     RDEDLRADRQPEFTQIDCEMSFVKQEDVLNLFEGMMKHLFRFVKNIDMTDAFPRMTWA
                     DAMKYYGSDKPDIRFDMQFVEIKELVSGKNFVVFDEADYVGAICASNCATYTRKQLDE
                     LTDFVKRPQIGAKGLVYIRCEADGTFKSSVDKFYTNDDLAKIATACKAEKGDLILILA
                     GATEKTQKALCELRLEMGSRLGLRDKNVFAPLWVIDFPLFQWDEETQRFYAMHHPFTC
                     PNEEDIALLDTNPGKVRATAYDMVINGVELGGGSIRIHESALQKKMFELLGFTPEQAE
                     AQFGFLMSAFKYGAPPHGGLAFGLDRLVSLFAGLDSIRDCIAFPKNNSGRDVMIDAPS
                     VIDNAQLDELGLRIELKKS"
     gene            complement(2914..>5293)
                     /locus_tag="PLD76_02715"
     CDS             complement(2914..>5293)
                     /locus_tag="PLD76_02715"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU26948.1"
                     /translation="TESFCARKQQTSFIPIDSPKTVRFSIHVPRIDSSLCLAIVGNQP
                     ALGNWDNKKALVMQDTHYPEWTAEMDASNVNNPIEYKYVLYNRTKKTIVSWEKGANRT
                     LEIDAVIANQLVLKNDETFRYDIPNFKAAGIALPIFSLRTENSFGIGEFADLKPLVDW
                     SAKTGQKVIQTLPINDTTRSRTNADSYPYSSITVLALHPAYINLIGIGKLKSKKEMDK
                     FAQIQESLKQFKTVKYQEVIEAKWEYFRLIYKQEKEKTFSSADYKQFFAENKQWLLPY
                     AAFCYLRDKHSDSNFRNWKNFSVFDWDKIDDFFKPEFADFDEAMIHIFLQYHAHKQLL
                     EASNYAKSKGIVLKGDIPIGISPDSVEAWMEPHLFNLDSQAGAPPDPFSATGQNWGFP
                     TYNWERMEKDGYQWWKTRFQKMAAYFQAYRIDHVLGFFRIWRMSADDVQGLLGHFDPA
                     LPLTADEIKGFGTWFDYDRMVKPYIREHVLNGLFGDYTNEVKERFLDIEQYGVYRFKK
                     AYNTQKKIEAFFIKNQDKMEEHKIAILQNGLFALFCEVLFIEDSKKQGTFHPRIAFHS
                     TYSYRDLDNGAKNALNNLYNHFYYQKHNDFWKEQAMKKLPALLQATNMLVCGEDLGMI
                     PECVPSVMHDLYIMSLEIERMPKDPKEEFIHMPNVPYLSVCTTSTHDMNPIRGWWEED
                     RGTTQRYFNNILNEWGEAPQFCEPWVCEKIVARHLHSPAMLVVLPFQDWLSINGKYRR
                     ENILEERINVPSDPKHFWCYRMHFTIEELLENDEVNDKILALTQQAGRNSTM"
BASE COUNT         1606 a          921 c         1099 g         1667 t
ORIGIN      
        1 cttgagaaaa tatttgatgc taagcaaggt acaccagaaa gtgatgaggc agacattttg
       61 ggtatgttaa tcgacgaata cgaaaagaaa cattatccta ttgaagcacc agacccgata
      121 gaagccatta agatacgtat ggaagaaatg caattaaaac aagcagacat ggtggagata
      181 attggtggaa agagtcgagt gtcagaagtg ttaaacagaa agaggagact aacagtaaat
      241 atgatacgaa atataacaaa acgattgaat atttcgccga atgtattgat aaacgactat
      301 aaactgagta gataaaatcc gacctgcaaa atagtataag gagtttcaat ctcttgtaaa
      361 tacaattttt cttttcttca tccttctcct acacgccgac tgacctcgca caatgagcct
      421 aaaaaccagt gatttggcgt aaagaaatag tcctgtttga gcgacaaagg agcgagtttg
      481 gactgtttta gccagcagag cgtcagtttt tagagcgagg agggcggagt cttgagtttt
      541 tttgattact tttttgtgtc aagtggcaca tcccgacttt agttcgggga caaaaaagtg
      601 attagggttt ggggcaaagc cccatatata ataagattac acaaaatata ttaaaccaaa
      661 aagcacctgc atataaaatg taagtgcttt tttaaattgt cggggtacca ggattcgaac
      721 ctgggaccct cccgatacac aatcgggatg tgctagcctt acaaagttat gtttattcct
      781 aaaaaccaaa aagcacctgc ataataaaat gtaagtgctt ttttaaattg tcggggtacc
      841 aggattcgaa cctgggaccc cctgctccca aagcaggtgc gctagccggg ctgcgctaca
      901 ccccgaaaat ctcgttaata agaggtatta tgtctcgctt aacgggatgc aaagataact
      961 atctttttta ttttagcaaa agattttgaa ataattttat aaaattttta taacatcgca
     1021 attttatgta cttttgtgtt ttctttttca caacatcata attatataaa gagagtttta
     1081 ttatgtacag aacacatact tgtggagaat tacgactatc acacatagga caacaggtta
     1141 cacttgctgg ctgggtacaa cgggtgcgta gaatgggtgg aatgattttt gttgatttac
     1201 gcgatagatt tggtattacg cagttagttt ttaaccaaga aacaaatgct gatttgtgtg
     1261 ctcaggcaaa tgaattggga cgtgagtttg tgatacaggt gcaagctgac gtggctgaac
     1321 gtagcaataa aaataagcat atccctacgg gtgaaattga attgcttgta tcgactatgc
     1381 gagtgctgaa tacttcggaa acacctcctt ttaccataga agataacaca gacggtggag
     1441 atgatttgcg tatgaagtat cgctacttgg atttgcgtcg taatacagtg cgtgaaaaca
     1501 tgatattgcg tcataaggtt gcgtttgaga caagacgcta tctcgaccaa atgggatttc
     1561 ttgaggttga aacaccgata ttaatcgctt cgacacccga aggagcaagg gattttgtgg
     1621 ttccatcgcg tatgaaccaa gggcagtttt atgccttgcc acagtctccg cagttgttta
     1681 aacaattgtt gatggtgtcg ggttttgata agtattttca gatagcaaaa tgttttagag
     1741 acgaggattt acgtgccgat cgtcagccag agtttacaca aattgactgc gaaatgtcgt
     1801 ttgtaaaaca agaagatgta ttgaatttgt ttgaaggaat gatgaaacat ttattccgtt
     1861 ttgtgaaaaa tattgacatg acagatgctt ttcctcgtat gacatgggct gatgctatga
     1921 aatattatgg ttctgacaaa ccagacatac gttttgatat gcaatttgta gaaataaaag
     1981 aacttgtatc gggcaaaaat tttgttgtat ttgacgaggc tgattatgta ggagccattt
     2041 gtgctagcaa ttgtgcaaca tatacccgca aacaattaga tgaactgacc gattttgtaa
     2101 aacgaccaca aattggagct aaaggcttgg tgtatatacg ctgcgaagca gatggtacgt
     2161 ttaagtcgtc agtagataag ttttatacca acgatgattt ggctaaaatt gcaacagcat
     2221 gtaaggctga aaaaggcgat ttgatactaa ttttggcagg tgccacagaa aaaacacaaa
     2281 aagccttgtg cgaattgcgt ttagaaatgg gtagccgatt gggtttgaga gataaaaatg
     2341 tgtttgcacc attgtgggta attgattttc ctttgtttca gtgggacgaa gagacacaac
     2401 gtttttatgc tatgcaccat ccatttactt gtcctaacga agaagatatc gcattgttgg
     2461 atacaaaccc aggcaaagta cgtgctacag cctatgatat ggtgataaat ggcgtagagt
     2521 tgggcggagg ttctatccgt attcatgaaa gtgcgttgca aaagaaaatg tttgaattat
     2581 taggatttac acccgaacaa gcagaggcac aatttggatt tttgatgagt gctttcaagt
     2641 atggagcacc tcctcatggt ggtttggctt ttgggttgga cagattggtg tcgttgtttg
     2701 ctggattgga tagcatccgc gattgcattg ctttccccaa aaataactcg ggaagagatg
     2761 tgatgataga tgcaccttcg gtaattgata acgctcagtt agatgagtta ggcttgcgta
     2821 ttgaattgaa aaagagctag atagttggat ataatgcgat aaaaaaagcg ttttgatgga
     2881 tgtcaaaacg cttttttact atttgggaga aaattacatt gttgagttgc gtccagcttg
     2941 ttgtgttaaa gcaagtattt tgtcgttgac ttcgtcattt tcaagcaatt cttcaattgt
     3001 aaaatgcata cggtaacacc aaaaatgttt tggatcagag gggacattga tacgttcctc
     3061 taaaatattt tctcggcggt atttaccgtt aatagataac caatcttgaa atggcaatac
     3121 aaccaacatg gcaggtgaat gcaagtgacg agctacaatt ttttcgcata cccatggttc
     3181 gcaaaattgc ggagcttctc cccattcgtt gagtatgtta ttaaaataac gttgcgtagt
     3241 acctctgtct tcttcccacc agccccgaat agggttcata tcgtgtgtag atgtggtaca
     3301 aacggataaa tagggtacat tcggcatgtg aataaattct tctttgggat cttttggcat
     3361 acgctctatt tccaagctca taatgtataa atcatgcatt acacttggca cacattctgg
     3421 aatcattcct aaatcttcgc cacaaaccaa catattggtc gcctgaagca aagctggaag
     3481 ttttttcatc gcttgttctt tccaaaaatc gttgtgtttt tggtaataaa aatggttgta
     3541 caaattattc aaggcatttt tcgccccatt gtctaaatcg cgatacgaat acgttgaatg
     3601 aaatgcaata cgtggatgaa atgttccttg ttttttgcta tcttcgataa acaaaacctc
     3661 gcaaaacaaa gcaaacaagc cattttgcaa aatagctatt ttatgctcct ccattttatc
     3721 ctgatttttg ataaaaaatg cttcaatttt cttttgcgta ttatatgctt ttttgaaacg
     3781 atacacacca tattgttcta tgtctaaaaa acgctctttt acttcgttgg tataatcgcc
     3841 aaataagcca ttcaatacgt gttcgcgaat gtaaggtttt accattctgt cgtagtcgaa
     3901 ccaagttcca aaacctttga tttcatcagc tgttaaaggc aatgcagggt caaaatgacc
     3961 taacaaacct tgcacatcgt cagctgacat tcgccaaata cggaaaaaac ctaacacgtg
     4021 gtcgatacga tatgcttgaa aataagctgc cattttttga aaacgagttt tccaccattg
     4081 gtatccgtct ttttccattc tttcccagtt ataggttggg aaaccccagt tttgaccagt
     4141 ggctgaaaaa gggtcggggg gtgcacccgc ttggctatct aagttaaata aatgtggttc
     4201 catccacgct tctacactat ctggactaat accaatagga atatcacctt tcagcacaat
     4261 gccttttgat ttagcgtaat tacttgcttc aagtaattgt ttgtgagcat gatattgcaa
     4321 aaagatatga atcattgcct cgtcaaaatc ggcaaattct ggtttaaaga aatcatctat
     4381 tttgtcccaa tcaaacacag aaaagttttt ccagtttctg aaattcgaat cactgtgttt
     4441 atctcttaaa taacaaaatg ctgcatatgg tagcaaccat tgtttatttt cggcaaaaaa
     4501 ttgtttatag tcagctgagc taaatgtttt ttctttttct tgtttgtata ttaaacgaaa
     4561 atattcccat ttagcttcta taacttcttg gtatttaacg gttttaaatt gctttaaaga
     4621 ttcttgtatt tgtgcaaact tatccatttc ttttttgctt ttcagcttgc caatacctat
     4681 aaggttgatg taagcaggat gtaaagccaa taccgtaata ctactatatg gatacgaatc
     4741 agcattggta cgcgaacgtg ttgtatcatt aattgggagc gtttgaatga ctttttgtcc
     4801 tgttttagct gaccaatcga caaggggttt taaatcagca aattcgccaa ttccaaagct
     4861 attttctgtg cgtaacgaga aaatgggtag tgcgatacca gccgctttaa agttgggtat
     4921 atcgtaacga aatgtttcgt cattttttag tactaattga ttagcaatta cagcgtcaat
     4981 ttccaatgtt ctattggcac ctttttccca tgatacgatt gttttttttg tgcgattata
     5041 tagcacgtat ttatattcaa ttgggttgtt tacgtttgac gcatccattt ctgctgtcca
     5101 ttcagggtaa tgggtatctt gcattactaa agcttttttg ttgtcccaat tacctaaagc
     5161 aggttgattg cccacaatag caaggcaaag cgaggagtct atacgaggta catggataga
     5221 gaatcgaact gttttgggcg aatcgatagg aataaaagat gtctgttgtt tacgagcaca
     5281 aaaactttcc gta
//
LOCUS       DAOSMY010000074         5259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_15817_length_5259_cov_3.577440, whole genome shotgun sequence.
ACCESSION   DAOSMY010000074 DAOSMY010000000
VERSION     DAOSMY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15817_length_5259_cov_3.577440"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="PLD76_02720"
     CDS             <1..1573
                     /locus_tag="PLD76_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU26949.1"
                     /translation="NDMQYLGDKRSIIVLGSGAYRIGSSVEFDWCSVNALETIRKEGW
                     RSVMINYNPETVSTDYDTCDRLYFDELSFERVMDIVELENPHGLIVSTGGQIPNNLAL
                     RLNNMNVPLLGTTATSIDRAEDRHKFSSMLDSLKIDQPRWKELSSLEDVQLFVDEVGF
                     PVLVRPSYVLSGAAMNVCSNKEELKEFLQLAANVSKKHPVVVSEFIESAKEIEMDAVA
                     DKGEIMMYAISEHIEFAGVHSGDATIQFPPQKLYLETMRRVKKIAHQIAKELCISGPF
                     NIQFLAKNNEIKVIECNLRASRSFPFVSKVLKINFIELATKIMLGIPVQKPNKNAFNL
                     DYVGIKASQFSFSRLQKADPVLGVDMASTGEVGCIGDDADEAILTSMLSVGYTIPKKN
                     ILLSTGTPKQKVDMLDAARLLLKKGFNLYATGGTHTFLQENDIPATLVHWPSEEMQPQ
                     ALEMLKSKQIDLVVNIPKNLTQRELDNGYKLRRAAIDFNIPLLTNARLASAFIHAFCH
                     LDINDVQIKSWNEYK"
     gene            1602..3479
                     /locus_tag="PLD76_02725"
     CDS             1602..3479
                     /locus_tag="PLD76_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU26950.1"
                     /translation="MEQLKHECGIAMIRLRKPLYFYQQKYGTWMYGLNKLYLLMEKQH
                     NRGQEGAGVACVKLDAHPGEEFMFRERAEGTSAISKIFADMHKQIREVANGDIDDVEY
                     ARQFVPFAGELYMGHLRYSTTGKAGISYVHPFMRRDNYRAKNLAICGNFNLTNVDEIF
                     NYISKKGQHPRKYADAFILLEQIGHRLDREVERVYALAKEEGKRGTDITHAIEEDIKI
                     ENVLQSSSWLWDGGYVMCGLTGSGESFVLRDPWGIRPAFYYADEEIVVTASERPVIQT
                     ALNLTASQVKELNPGEAMILDKTGNLRLVQVLQPKIKQACSFERIYFSRGSDCDIYNE
                     RKALGENVVPGILKSIDNDLKNTVFSFIPNTSEVAFYGMIQCLNERLNDIKNQQIRQY
                     GRRLTSEMLDEILSMSIRSEKVAIKDIKLRTFISEGNSRNELAAHVYDVTYGSIVPNQ
                     DNLVVIDDSIVRGTTLKQSIIKILARLQPKKIVIVSSSPQVRYPDFYGIDMSNMDEFV
                     AFRAAIALLKDNNMQHVIDDVYKKCKQQENQHKEEIENYVKQIYAPFTAEQVSEKIVE
                     LLKPEDVQCPVELVYQSLEGLLKSCPNHTGNWYFSGDYPTQGGKRLVNESFIRYYEKN
                     Y"
     gene            complement(3539..4012)
                     /locus_tag="PLD76_02730"
     CDS             complement(3539..4012)
                     /locus_tag="PLD76_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HOU26951.1"
                     /translation="MYTWFECKVRYEKIVEGKTKKVTEPYLIEAFSYTEAEARVMQEL
                     SPYISGDYSITNIKRAKIAELFPNDLGDRWYTCKINIILLDESRGIEKKVANYILVQA
                     SAVLDAWQKLEIGMKGTLSDYVVVSIGESPIIDIFPYKKEEMKQVISSTNVSIEE"
     gene            4264..>5259
                     /gene="gap"
                     /locus_tag="PLD76_02735"
     CDS             4264..>5259
                     /gene="gap"
                     /locus_tag="PLD76_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU26952.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIQVVAVNDPFLDLDYLVYM
                     LQYDTVHGKFKGTAENKDGKLIVNGKEVKFYAEKDPAAIAWGAAGADYVVESTGVFTT
                     IDKAKAHLAGGAKKVVISAPSNDAPMFVCGVNLDAYKADMDIVSNASCTTNCLAPLAK
                     VINDNFGIVEGLMTTVHAATNTQKTVDAPSAKDWRGGRSILGNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMAFRVPTADVSVVDLTVRLEKAASYDEIKATIKKASENELKGILGYTE
                     DSVVSTDFTTDSRTSIFDAGAGIALNGNFVKLVSWYDNEWGYSNKVLDLIAHMDSV"
BASE COUNT         1687 a          872 c         1046 g         1654 t
ORIGIN      
        1 caacgatatg cagtatttgg gcgataaacg ctctattatt gtacttggct ctggagcata
       61 ccgcattggt agctcggtag agttcgactg gtgtagtgta aatgccctag aaactatacg
      121 caaagaaggt tggcgttcgg taatgataaa ttataacccc gaaacggttt ctacggatta
      181 tgatacttgc gaccgtttat atttcgacga attatcgttc gaacgtgtaa tggatatcgt
      241 agaattagaa aatccacatg gattaatcgt ttctacaggt ggtcaaattc caaataactt
      301 agctttacgt ttaaataaca tgaacgtgcc attgttgggt acaacagcca ccagtattga
      361 tagagctgag gatagacata agttctcgtc gatgttggat agtttaaaaa ttgaccagcc
      421 acgttggaaa gagttgtctt cgctcgaaga tgtacaactt tttgtcgacg aagtaggttt
      481 ccctgtgctt gttcgtccat cgtatgtgct ttctggagcg gcaatgaatg tgtgttctaa
      541 caaagaagaa ctgaaagaat ttttgcaatt agctgccaat gtgtctaaga aacaccctgt
      601 ggtagtaagc gaatttatcg aaagtgccaa agaaatcgag atggatgcgg tagccgataa
      661 aggggaaata atgatgtatg ccatttccga acacatcgag tttgctggtg tacactctgg
      721 tgatgctact attcagtttc ctcctcaaaa attgtactta gaaacaatga gaagggtgaa
      781 aaaaatcgct caccaaattg cgaaagaact atgtatctca ggtccattta atattcaatt
      841 tttagctaaa aataacgaga ttaaggttat cgaatgtaat ttgagggctt ctcgtagttt
      901 tccatttgta tctaaagtgc taaaaattaa ctttattgaa ctagcaacca aaattatgtt
      961 gggcatacct gttcaaaaac cgaacaaaaa tgcctttaac ttagattacg taggtatcaa
     1021 agcttctcaa ttctctttct ctcgtttgca aaaagctgac ccagtattgg gtgtggatat
     1081 ggcttcaaca ggcgaagtgg gatgtattgg cgatgatgct gatgaggcaa tattaacttc
     1141 tatgctatct gtaggttata ctattccgaa aaagaatatt ttattatcaa caggtacacc
     1201 aaaacaaaaa gtagatatgc ttgatgcagc tcgtttgttg cttaaaaaag gctttaatct
     1261 atatgcgacg ggtggtacac acaccttctt gcaagaaaat gatatacctg ctaccttggt
     1321 gcattggccc agcgaagaaa tgcaaccaca agctcttgaa atgctcaaaa gcaaacagat
     1381 tgacttggtg gtaaatatcc ctaagaattt aacgcaaaga gaactagata acggatataa
     1441 acttcgtcgt gcagccattg attttaatat tccactatta acaaacgctc gtttagcctc
     1501 tgcatttata catgcatttt gtcatttaga tatcaatgat gtacaaatta aaagctggaa
     1561 cgaatacaaa taattgattt ttcgacccta acataaccac aatggaacaa ctaaagcatg
     1621 aatgcggtat agcgatgatt cgcctgcgta agcccctgta tttttaccaa caaaaatacg
     1681 gcacgtggat gtatggctta aacaagcttt acttattgat ggagaaacaa cacaatcgcg
     1741 ggcaagaagg tgctggtgta gcatgtgtca aattagatgc tcatcctggt gaggaattta
     1801 tgtttcgcga acgtgccgag ggtacaagtg caataagcaa gatatttgct gatatgcata
     1861 agcagattag ggaagttgcc aatggcgata ttgatgacgt agaatatgct cgtcagtttg
     1921 tgccttttgc tggcgaatta tatatggggc acctccgtta tagtaccacg ggtaaagctg
     1981 gaatttcgta tgtacatcct tttatgagaa gggataatta cagagcaaag aatttagcta
     2041 tttgtggtaa ttttaacttg accaatgtag atgaaatttt taattacata tctaaaaaag
     2101 ggcaacatcc gcgtaaatat gccgatgcgt ttattctatt ggagcaaatt gggcatcgcc
     2161 ttgatcgtga agtagaacgt gtatacgcct tagccaaaga agagggtaaa agaggtactg
     2221 atataacaca tgctattgaa gaagatatta agattgaaaa tgttttacag tcatcttctt
     2281 ggctgtggga tggtggatat gttatgtgtg gtttaacagg aagtggcgaa agttttgtgc
     2341 ttcgggatcc atggggaatt cgccccgcct tctattatgc tgatgaggaa attgtagtta
     2401 ctgcctctga aagacctgta atacaaacag cattaaatct tacagcaagt caggtaaagg
     2461 aactaaaccc aggagaagca atgatcctcg ataaaacagg aaacttgcga ctagtgcaag
     2521 tattgcaacc caaaatcaaa caagcatgtt cgttcgaacg tatttatttt tcgcgtggta
     2581 gcgattgtga tatttacaac gaacgcaaag cgttgggtga aaatgtagtg ccaggtattc
     2641 ttaaaagtat tgataatgat ttaaaaaata ctgttttttc atttattccc aacacttctg
     2701 aggttgcttt ttatggaatg attcaatgtc ttaacgaacg tttgaatgat attaaaaatc
     2761 aacaaattcg tcagtacgga cgacggttga cttctgaaat gttagatgaa atattatcca
     2821 tgtctattcg ttcagaaaaa gtagcaataa aagatatcaa gttgcgtacg tttatttccg
     2881 aaggcaatag tcgcaatgag ttagctgctc acgtgtacga tgttacgtat ggtagcattg
     2941 tgcctaacca agataattta gtcgttattg atgatagtat tgtgcgtggt actaccctta
     3001 aacaaagtat cattaaaatc ctagcacgat tgcaaccaaa aaagattgta attgtatcat
     3061 catcaccaca agttcgttat cctgattttt atggtataga catgtcgaat atggacgagt
     3121 ttgtagcttt tcgtgcagcg attgcattgc tcaaagacaa taacatgcaa catgtcattg
     3181 atgatgtgta caaaaaatgt aagcaacaag aaaatcagca taaggaagaa atagagaatt
     3241 acgttaagca aatatatgcc ccttttacag cagaacaagt ttctgaaaaa attgtagaat
     3301 tactcaaacc cgaagatgtg caatgcccag tggaattggt ataccaaagt ttagagggct
     3361 tgttgaaatc atgtcctaat catacaggta attggtattt ttctggagat tatcctacac
     3421 aaggaggtaa acgtttagtc aatgaatcgt ttattcgata ctacgaaaaa aattattaaa
     3481 taaaaatagt cctcgatgtt atatcgagga ctatttttat tactattgta tagttatctt
     3541 attcttctat tgatacattc gtcgaactaa ttacttgttt catttcctct tttttatacg
     3601 ggaaaatatc aattattgga ctctctccta tcgatacaac cacataatca gataaggtgc
     3661 ctttcatgcc tatttctaat ttttgccaag catctaacac ggcagaggct tgtaccaata
     3721 tgtaattagc tactttcttt tctatacctc tactttcatc aagcaaaatg atattaattt
     3781 tacacgtata ccatctgtcg cccaaatcgt ttggaaaaag ttctgcgatt ttggcacgtt
     3841 taatgtttgt gattgaataa tccccactta tatatggcga taattcttgc attacgcgag
     3901 cttccgcttc tgtgtacgaa aaagcttcaa ttaaataagg ttctgttact tttttagttt
     3961 ttccttctac tattttctca tagcgtactt tacactcaaa ccatgtatac atattattgt
     4021 tgtttttaat tagaatgcaa aaatacagat aataatgagc ctcaaaagaa aattaggaaa
     4081 atagttttcc acaaaattgc agttacttgt ttacaagcct aatagcattt attaataaac
     4141 caaatatggg ttaacaaatc tctatttttt ttgaaaaaaa acaaacaatt ctttggttag
     4201 taataaaaaa aaaagtaatt ttgcatggtt taaaaaaata acttaattta taaataagaa
     4261 aaaatgatta aagtaggtat taatggattt ggccgtatcg gacgtttagt gttccgtgca
     4321 gctcaagaaa gaaacgacat tcaagttgtt gcagtaaatg acccattttt ggatcttgac
     4381 tatttagttt atatgttgca gtatgatact gtacacggta aatttaaagg tacagctgaa
     4441 aataaagatg gtaagttaat cgtaaacggt aaagaagtaa aattctatgc cgaaaaagat
     4501 cctgctgcta ttgcttgggg tgctgctggt gctgattatg ttgttgaatc aacaggtgtt
     4561 ttcactacta tcgacaaagc aaaagctcac ttagcaggtg gtgctaaaaa agtagttatc
     4621 tctgctcctt cgaatgatgc tcctatgttt gtgtgtggtg ttaacttaga tgcatacaaa
     4681 gctgatatgg atatcgtatc aaatgcatct tgtactacta actgtttggc tcctttggct
     4741 aaagtgatta atgataattt tggtattgta gaaggtttga tgacaactgt acacgctgct
     4801 actaatactc aaaaaacagt tgatgctcct tctgctaaag attggagggg tggtcgttca
     4861 atcttaggta atattattcc atcttctaca ggtgctgcaa aagcagtagg taaagttatc
     4921 ccttcattga atggcaaact tacaggtatg gctttccgtg taccaacagc tgacgtttct
     4981 gtagttgact taactgtgcg tttagaaaaa gctgcttcgt acgacgaaat taaagctact
     5041 atcaaaaaag cttctgaaaa tgaattgaaa ggtattttag ggtatactga agactcagta
     5101 gtttctactg actttactac cgactctcgt acttcaattt ttgatgcagg tgcaggtatt
     5161 gctcttaatg gtaacttcgt gaaattagtt agctggtatg acaatgagtg gggttattct
     5221 aacaaagtgt tagatttaat tgcacacatg gattcagtt
//
LOCUS       DAOSMY010000075         5156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_16252_length_5156_cov_2.953931, whole genome shotgun sequence.
ACCESSION   DAOSMY010000075 DAOSMY010000000
VERSION     DAOSMY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16252_length_5156_cov_2.953931"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1928)
                     /gene="argS"
                     /locus_tag="PLD76_02740"
     CDS             complement(135..1928)
                     /gene="argS"
                     /locus_tag="PLD76_02740"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU26953.1"
                     /translation="MNLTQQVENFILQAIPQLYHQEIDISLVQIQKTKKDFDGELTVV
                     TFPFVKLARKSPEAIGVELGEWLVANAPIIKSFNVIKGFLNLSINPRYWLQMLQMITQ
                     DPYYGSKLPKPDAPVMMIEYSSPNTNKPLHLGHIRNNLLGYSIAEIQKANGWNVVKTN
                     IVNDRGIHICKSMLAWQKFGNGETPESSGMKGDHLVGKYYVIFDKEYKKQVADLLQQG
                     MSEDEAKKKAPLMIEAQEMLRKWENDDVTVRQLWEMMNKWVYAGFDQTYKQLGVDFDK
                     IYYESETYLEGKNKVEEGLSKGVFYKKEDGSVWADLTQDGLDEKLLLRADGTSVYMTQ
                     DIGTAKLRYDDYPIQKMVYVVGNEQNYHFQVLALLLDKVGFSWGKDLVHFSYGMVELP
                     EGKMKSREGTVVDADDLMQEMVQTALDTANELGKLDGLSVDEVQSIANMVGLGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTHARIQSVLRKAIDQGISISGEVQLQQD
                     LAEKEISLLRLLADFPAVIEQAGNDFSPALIANYAYDLVKEYNQFYHDFSILKETNVA
                     VRHFRLVLSREIARVIKSSMRLLGIDVPDRM"
     gene            complement(2089..2361)
                     /locus_tag="PLD76_02745"
     CDS             complement(2089..2361)
                     /locus_tag="PLD76_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU26954.1"
                     /translation="MNKAELISAIADNSGLSKVDSKKALDGFIAAVSNALKSGDKVAL
                     VGFGSFDVAERSARTGVNPATGAKIKIDAKKVVKFKAGAELADLVK"
     gene            2568..3323
                     /locus_tag="PLD76_02750"
     CDS             2568..3323
                     /locus_tag="PLD76_02750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU26955.1"
                     /translation="MNNSFLGGIPPVVKNLLIINILFWLASLTLPGVIGIDVVDWLGL
                     HYWQSDKFVVSQLVTYMFLHDTSSLFHLFFNMFALYMFGKNIEYIWGTKKFLLFYFVT
                     GIGAAIVQQITWSFDFVPIANAFGEYVNNRDISVLMPYLNVNESTVLSTTEVLQLKNM
                     VLNQPITVGASGAVFGILLAFAMLFPDARIMLLFLPVPIKARYFVIFYGAMELFLGVS
                     NFQGDSIAHFAHLGGMLFGLVLILLWRKKGTLY"
     gene            3355..4239
                     /locus_tag="PLD76_02755"
     CDS             3355..4239
                     /locus_tag="PLD76_02755"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU26956.1"
                     /translation="MWSQIKTNFKQGSYLTRLLYINLGMFVLVRLVLLIFTLFKHDIS
                     FAIRYIEMPAYIGNWITQPWGIITYMFLHVDFIHFIFNVLVLYWYGTIFLQFFNQKQL
                     VGVYILGGIGGAFLYVFGYSVFPYFQEVLKHTYLLGASASVMALIFTTVIYAPNYEVQ
                     LALIGRVKLKYIGLVLFAVDVLGVTSVNAGGSIAHIGGAITGILFASLYVRQRVDLTT
                     GISWLLDKLATLVQPKPKITVSYGRPKTDAEWNVEKKAKNAALDEILEQIKKSGYDNL
                     SAEQKKMLFDISNKKNNN"
     gene            4255..>5156
                     /locus_tag="PLD76_02760"
     CDS             4255..>5156
                     /locus_tag="PLD76_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU26957.1"
                     /translation="MKLKWLTNAFLLLLNSIVLVILLVINYVSVLSPDSVWSVLAPTN
                     ILFTAFVSINLLFVLWWIIHLKWYAIFSVLALLLCYTNISNTFIIWSEKPFPEENKPI
                     YSVVSYNIHHFKFFPKEKETKTGNHILDYLVELDADIICLQEFAYSDYEPFTLSDIKN
                     QLSDYPYNHIEILYKDRHISKGIATFSKHKITNKQHISIPAEYHGAISSDVLLDGKKI
                     RIINFHFESNRLTSKDKDIKRLTDRNKIPSFAKRLQSKLHTASRKRGKQAEALMTFAT
                     NSNMPTIICGDMNDVPSSYTYRTIT"
BASE COUNT         1597 a          907 c          828 g         1824 t
ORIGIN      
        1 ctatcgttgc cttgtaataa tctccttttc tgtatgcttt ttgagccgta ttacaagcag
       61 tagtagcaag taatgctatc aaaccaattg cgacaaatga gttaaatttt ttcatatgca
      121 ttcattttaa agtgttacat tctatctggc acatcaatac ctagtagcct catactcgat
      181 tttataacac gtgctatttc acgtgataat accaagcgaa aatgacgaac agctacattt
      241 gtttctttta aaattgaaaa atcatggtaa aactggttat attcttttac caaatcatac
      301 gcataatttg caatcaatgc aggactaaaa tcatttcctg cttgttctat tacagcaggg
      361 aaatcagcca atagacgcaa taaactaatt tctttttcgg ctaaatcttg ttgtaattga
      421 acttctcccg aaatagaaat accttgatct atagctttac gcaatacgga ttgaatacga
      481 gcatgtgtat actgaataaa tggtcccgta tttccattaa aatcaattga ttcttttggg
      541 ttaaacgtca tatttttgcg agggtcaact tttagtataa aatactttaa cgcacctaaa
      601 cctaccatat tggctatgga ttgcacttca tccacggaca aaccatctaa tttccctagt
      661 tcattagctg tgtctaatgc tgtttgtacc atttcttgca tcaaatcatc agcatctaca
      721 acagtacctt cacgactttt cattttccct tcgggcagtt ctaccatacc atacgaaaag
      781 tgcactaaat ctttacccca cgaaaagccc actttatcca acaaaagtgc taatacctga
      841 aaatgataat tttgttcatt cccaaccaca tacaccattt tttgaatggg ataatcgtcg
      901 taacgcaatt tagctgtgcc aatatcttgg gtcatatata cggatgtgcc atctgcacgc
      961 aatagcaatt tttcgtctag cccgtcttgt gttaaatccg cccacaccga accatcttct
     1021 tttttataaa aaacgccttt tgataaacct tcttctactt tattttttcc ctcaagatac
     1081 gtttcagatt cgtagtatat tttatcaaaa tcaacaccga gttgtttgta tgtttggtca
     1141 aaacctgcat acacccattt attcatcatt tcccaaagtt gacgtactgt tacatcatca
     1201 ttttcccatt ttctcaacat ttcttgggct tctatcatta atggagcttt ctttttagct
     1261 tcatcttcag acatgccttg ttgcaacaaa tcggcaactt gctttttata ctctttgtca
     1321 aaaattacat aatattttcc gactaaatgg tcgcccttca tcccagaaga ctctggcgtt
     1381 tcaccattac caaatttttg ccaagccaac atggatttac aaatatgaat ccctctatca
     1441 tttacaatat tggttttaac tacgttccaa ccattcgctt tttggatttc ggcaatactg
     1501 taccccaata gattattacg tatatgtcct aaatggagtg gcttgtttgt attgggtgat
     1561 gaatattcaa tcatcattac aggagcatct ggctttggca atttacttcc ataatatgga
     1621 tcttgtgtaa tcatttggag catttgcaac caatagcgtg ggtttatact caagtttaaa
     1681 aagcctttaa tcacattaaa actttttatt ataggagcat ttgctactaa ccattctcct
     1741 aactctaccc caatagcttc gggggattta cgtgctaatt taacaaaagg gaacgttacc
     1801 accgtaagtt ctccatcaaa atcttttttt gttttttgta tttgcaccaa agaaatatca
     1861 atttcttgat gataaagttg aggaattgct tgtaatataa aattttctac ttgttgggta
     1921 agattcatgg atatatatag ctaaaatgat acggaattat ataagtactt aaactgaaca
     1981 aaaatactat tttttttggg aagaaaatag tagtaaataa aaaaaatgga gctcaatact
     2041 tttgaaaccc cattttttta gaatatttag tgttttctat ttgctatcct atttaactaa
     2101 gtcagccaat tcagcacctg ctttaaattt aacaactttt ttagcatcaa ttttgatttt
     2161 ggcacctgta gctggattaa cacctgtacg agcacttctt tcagctacgt cgaaagaacc
     2221 gaaaccaaca agggcaactt tatctccgga ttttaatgcg ttagatactg ctgcgatgaa
     2281 accatcaagc gctttttttg aatcaacttt gctaagacct gaattgtcag caattgcact
     2341 aataagttct gctttgttca taatgttttt gatttagagt ggttaaaaaa ataaattaat
     2401 taaaaataaa atttcatact ttggcaaatg taattgaaaa ttaattaatt gcaatacttt
     2461 tttaaaaaaa aatatacttt ttggtgaaaa tttccccaaa ctgtggcttt ttcagggttt
     2521 ttccataaaa aaataactac ttttgtacat caattaagta ggaagttatg aataattctt
     2581 ttctaggagg aatacctcct gttgtaaaaa atttacttat tatcaatatt cttttctggt
     2641 tggcttctct caccctacct ggtgtaatag gtattgatgt cgttgactgg ttaggcttgc
     2701 attattggca atcagataag tttgtggtat ctcagctagt tacgtatatg tttctacatg
     2761 atactagctc gttgtttcat ttatttttca atatgtttgc cctgtatatg tttggaaaaa
     2821 acattgaata tatttgggga acaaaaaagt ttctactttt ttattttgta acaggcattg
     2881 gagctgccat tgtgcaacaa ataacatggt cgtttgattt tgttcctatt gctaatgcct
     2941 ttggcgaata tgtaaataat cgtgatatta gtgtattaat gccttactta aatgtaaatg
     3001 aatctaccgt attgtcaacc actgaagtgt tgcaactaaa aaatatggta ctgaaccagc
     3061 ctattacagt tggagcttca ggtgcagtgt ttggtatatt gcttgcgttt gctatgttat
     3121 ttcctgatgc acgtattatg ctcttatttt tacctgtgcc cattaaggca agatactttg
     3181 ttatttttta tggtgctatg gaattgtttt taggtgtaag taattttcaa ggagatagta
     3241 tagctcattt tgctcatttg gggggtatgt tattcggctt agtgttaatt ctgctttggc
     3301 gtaaaaaagg caccttatac taatggttta aacactaaag aaaaaaaaat aactatgtgg
     3361 tctcaaataa aaactaattt taaacaaggc tcttatctta cacgcttgtt gtatatcaac
     3421 ttgggcatgt ttgtattggt tcgtttggta ttattgattt ttaccttgtt caaacatgat
     3481 atcagttttg ctatacgata tatagaaatg cctgcatata taggaaattg gatcacacaa
     3541 ccatggggca tcattaccta tatgttttta catgtagatt ttattcactt tatatttaat
     3601 gttttggtat tgtactggta tggcaccatt tttttgcaat ttttcaacca aaaacagtta
     3661 gtaggtgtgt atatattggg aggtattggt ggtgcattcc tatatgtttt tgggtattct
     3721 gtgtttcctt atttccaaga agtattgaaa cacacgtatt tgctaggtgc ttcggcatcg
     3781 gtgatggctc tgatttttac tacggtgata tatgctccta attatgaagt gcaacttgct
     3841 ttaattgggc gtgttaaatt gaaatacatt ggtttagttt tatttgcagt agatgtatta
     3901 ggtgtaacct cagtaaatgc tggagggagt attgctcaca ttggaggtgc cattacgggc
     3961 atattgtttg ctagtttata tgttcgccaa cgagtcgatt taactacggg cattagttgg
     4021 ttacttgata aactagctac cttagtgcaa ccaaaaccaa aaattaccgt aagctatggc
     4081 agaccgaaaa cagatgccga atggaatgta gaaaaaaaag ctaaaaatgc agctcttgat
     4141 gaaattttag aacaaataaa aaaatcaggt tacgataatt tgagtgctga acaaaaaaaa
     4201 atgctgtttg acattagcaa taaaaaaaac aataactaaa aaataccgac caacatgaaa
     4261 ctcaagtggt tgacaaatgc atttctttta ttgctcaata gcattgtatt agtgattttg
     4321 ttagtaatca attatgtttc tgtattgtct cccgactcgg tatggtcagt attggctcct
     4381 actaacattc ttttcacagc ctttgttagt atcaatttat tgtttgtact atggtggatt
     4441 atccatttga agtggtatgc tattttttct gttcttgccc tactcctttg ttatacaaat
     4501 attagtaata cgtttattat ttggtctgaa aagccttttc cagaggaaaa taaacctata
     4561 tactccgtgg tttcgtacaa tatacaccat tttaaatttt tccctaaaga aaaagaaacg
     4621 aaaacaggca atcatattct ggattatttg gtagaacttg atgccgatat aatctgcttg
     4681 caagaatttg catatagtga ttacgaaccg tttacattat ctgacataaa gaatcaactg
     4741 tctgattatc catacaatca tatagaaata ttatataaag acagacatat ctcaaaaggt
     4801 attgccactt tttcaaaaca caaaataacg aataaacaac acatttctat ccctgcagaa
     4861 tatcatggag ccatttcctc tgatgttctg ctagatggca aaaaaatccg cattattaat
     4921 tttcactttg aatcgaacag gttaaccagc aaagacaaag atataaaacg cttaactgat
     4981 cgaaataaaa taccatcgtt tgctaaaaga ttgcaatcga aacttcatac tgcgtcacgc
     5041 aaaagaggca aacaagcaga agcattaatg acattcgcaa caaactctaa catgcctact
     5101 attatatgtg gagatatgaa tgatgtccct tcttcgtata catatagaac gattac
//
LOCUS       DAOSMY010000076         5141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_16319_length_5141_cov_3.041880, whole genome shotgun sequence.
ACCESSION   DAOSMY010000076 DAOSMY010000000
VERSION     DAOSMY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16319_length_5141_cov_3.041880"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..866
                     /locus_tag="PLD76_02765"
     CDS             150..866
                     /locus_tag="PLD76_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOU26958.1"
                     /translation="MQKIHVITPVKDSIELSLQTIQSVCNSQTSMPFVYTVYNDFSTE
                     ENTQKLQKASQEFGFELINLSSITSTPSPNYLLVLQLAQQKAIDEDAALVIVESDVLV
                     QTNTLQALIDTAQQKTDAGMVASITVDESGNVNYPYLFAKKIKGLVKPTNKHVSFCCT
                     LLSLRYLKAYSFTSLQPDKNWYDVHISRQSRQLGFVNYLLGNHPVVHRPHSSRPWKQL
                     KYSNPIKYYWLKITQQKDKI"
     gene            866..2353
                     /locus_tag="PLD76_02770"
     CDS             866..2353
                     /locus_tag="PLD76_02770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU26959.1"
                     /translation="MQQVAFLPKVSVVIPIYNMELYLEETILSVLASSYTDYEVLLID
                     DGSTDNSVSIAKRFANAYPHIYFYEQPNQGVSAARNNAIKWSKGDYILPVDADNLIGK
                     EYISEAVHVLETYPNVKVVTCEAEFIGEKSGKWKQLPFSLSLLARKNMIDNCAMYRKS
                     DWQDCGGYCEEILGREDWDFWISMLKNGGDVVRLPIVGLYYRVRSNSKRRKTQHRKKK
                     LIDLLNVRHADFFEKQLHGRLHYNRTYSKLFNWIEKRICKRTTVIHPTYSQLAPCIER
                     LPLAFLVNNNVIHQGRNTLKQFSENGLDLVVKSYQIPHIINRLSYGFFRSSKAKRAYE
                     YAIILQQQAIGTPQAIAYIEQRFAGLLYQSYFVSVRSTCPYTFNTLIQQPSYEYRTLV
                     LQEIGRFTADLHTKGMLHQDYSGGNILFDVQNGKVLLELVDLNRIVFKHTIGIEEGCK
                     NFERLNIDEEALQILATEYAKARNFDVDMCVESVLKMRWHKHKQR"
     gene            2372..3184
                     /locus_tag="PLD76_02775"
     CDS             2372..3184
                     /locus_tag="PLD76_02775"
                     /inference="COORDINATES: protein motif:HMM:NF025082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU26960.1"
                     /translation="MKIYSLLLVKNEADIIASILKAAAQWSDKVIVMDNGSSDETWEI
                     VQQLATEYPQIIPFAKDDQAFQIGLRAVMFDAFKHELTASDWWCIRLDADEFFVENPR
                     DFLATIPKRYKQVCKASIDYCLTHEDVEEYTFTGDFEQDKHKIRYYQPYTWAELRFLR
                     HSKKLQWETGERRPEPCGLTYPKQIKVLHYQFRSPQQMQQRYAVRQQAKANGCGSFRH
                     EKGNDWKDYLRNRNELVYDKQDGTYQTLGNRNKFNKWHTRITKGFLTLFGYY"
     gene            complement(3147..4388)
                     /locus_tag="PLD76_02780"
     CDS             complement(3147..4388)
                     /locus_tag="PLD76_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU26961.1"
                     /translation="MLEKFRHYVHSINLLCGLFLMASLPFYYPKQQLALTLFFISYIV
                     EFFVDMKWKHIKLSATTWYFIGILLFFTMGFLYYPFEDSHTYFHNLTERRLALFGFAI
                     IGIFGYNSLYKLSYFLHTIIISSTLLILYLIIYHIGLPEFLTNENRYDLFTQARIAFV
                     NSHMAFNLYLNIALASVWYLAVTRFKELHWSVKTIYGFSILLIISTLLMSEGRSGFIA
                     AIGLSAIIVIRKIWLTKKIVAIGAFVFILIGMVFAVLQHERMSEKMIEQEPRLFLWTE
                     VALPIIQEKPILGHGLSDGQTQIDSVRQGKQSKILYELWKTNKRVDVHNQFLQTTIEF
                     GLIGLVLLLAIYYYPIRLVNRSRMTLLLLGLFLFSWQSLFDTFITGQFAIIFGFFITL
                     LLTVPKSESLNNNQTALKNLW"
     gene            complement(4397..>5141)
                     /locus_tag="PLD76_02785"
     CDS             complement(4397..>5141)
                     /locus_tag="PLD76_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU26962.1"
                     /translation="AYNLGVTKSKFPYLCFVHEDVFFRSSDWGLNIIEHLQTPQVGLI
                     GLAGGCLASRIPASWSTYGLKMNLIQSGKGKSSYIRTPNQPESTREEVVLLDGVFLAS
                     TKENFQFLQFDEKISGFHGYDYDICMQAKANNLRNYVVYDILLEHFSAGSHNTDYYKN
                     LLLVYKKWINQLPFSCTHLSEAETKLIEKKKLKKLIRKLGIRNFTYSEIKQELDYFAT
                     ITDNQKVLTWFGFKLFYARLFNNPSRLFK"
BASE COUNT         1735 a          864 c          957 g         1585 t
ORIGIN      
        1 aaaatacatt atttatttgg ttttttttga ctttttgctg tttttttgct cttctagtca
       61 ttctgtttta cttttctata aaaaaatatg tacttttgca tcactaagca cttttgcttc
      121 tctctatata taattttgat gtaatccaga tgcaaaaaat tcatgttatt acacccgtta
      181 aagattcgat tgaattaagt ttacaaacca tacagtctgt ttgtaattcg caaacttcta
      241 tgccttttgt atatacggtg tacaacgatt ttagcacaga agaaaataca caaaagttgc
      301 aaaaagcatc gcaagagttt ggttttgagt tgattaattt atcgtcaatt acttctactc
      361 cttctccaaa ttatttgttg gtgttgcaac tcgctcagca aaaagccatt gatgaagatg
      421 ccgcattggt tattgtagag tcagatgtgc tggtgcaaac gaacacacta caagctttaa
      481 tagacactgc acaacaaaaa acggatgctg gaatggtggc ttctattacc gtggacgaaa
      541 gtggaaatgt aaattatcca tacttgtttg caaaaaaaat aaaagggcta gtaaaaccga
      601 ccaataagca tgtgagtttt tgttgtacac tcttgtcgtt acgctattta aaagcctata
      661 gctttacctc attacaacca gataaaaact ggtatgatgt gcatatttct cgtcaatcac
      721 gccaattggg ttttgttaat tatttattgg gaaaccatcc tgtggtacat cgcccacata
      781 gtagtcgccc atggaaacaa ttgaaatatt cgaatccaat taaatattac tggctaaaaa
      841 ttacccaaca aaaagacaaa atttgatgca acaagtagcc tttttaccta aagtttcggt
      901 cgtaattcct atttacaaca tggaactata cctagaagaa accattttgt cggtacttgc
      961 gtcatcttat acagattatg aggtgttact aatagacgat ggttctactg ataattcggt
     1021 ttctattgcc aaacgttttg ccaacgcata ccctcatatt tatttttacg aacaacctaa
     1081 ccaaggagta tcggcagcta gaaataatgc cattaagtgg agcaaaggcg attatatttt
     1141 gcccgttgat gctgataatc tgattggtaa agagtatatt tcagaggcgg tacatgtatt
     1201 agaaacttac cccaatgtaa aagtagtaac gtgcgaagct gagtttatag gcgaaaaatc
     1261 gggaaaatgg aaacaattac cctttagctt gtcattgctt gcacgtaaga atatgattga
     1321 caactgtgcc atgtatcgta aaagtgattg gcaagattgt ggtggttatt gcgaggagat
     1381 tttgggaagg gaagattggg atttttggat ttcgatgctc aaaaatgggg gagatgtggt
     1441 gcgtttgccc attgtggggt tatattatag ggtgcgttcc aattctaaac gccgtaaaac
     1501 acaacaccgt aaaaagaaat taattgattt gttgaatgtg cgtcatgcgg atttttttga
     1561 gaaacaattg cacggtcggt tacattataa tcgtacctat tcgaagcttt ttaattggat
     1621 agaaaagcga atatgcaaga gaacaaccgt tatacatcct acctattcgc aactggctcc
     1681 atgtattgaa cgactgccat tagcgttttt agtaaataat aatgttatcc atcaaggcag
     1741 aaataccttg aaacaatttt ctgaaaatgg cttggatttg gtcgtgaaat catatcaaat
     1801 tccacatata atcaatcgat tatcgtatgg attttttcgt tcatcaaaag ccaagcgagc
     1861 ctatgaatat gccattatct tacaacaaca ggcaattggt acaccacaag cgattgcgta
     1921 catcgagcaa cgctttgcag gcttactcta tcagagttat tttgtaagtg tgcgttctac
     1981 gtgtccgtac accttcaata cattaattca gcaaccttcg tacgaatatc gtacattggt
     2041 tttgcaagaa ataggcagat ttacagctga tttacataca aaaggaatgt tgcatcaaga
     2101 ttattcggga ggtaatattt tatttgatgt acaaaatggc aaggtattac ttgaattagt
     2161 cgatttgaat agaattgtgt ttaagcacac gattggaatt gaagaaggtt gcaaaaattt
     2221 tgaacgttta aatattgacg aagaagcttt gcagatactg gctactgaat atgccaaagc
     2281 tagaaacttt gatgtggata tgtgtgtgga aagtgtacta aagatgcgtt ggcataaaca
     2341 taaacaacgt taagttaaac agttagttta gatgaaaata tacagtttac tccttgttaa
     2401 gaatgaagca gatatcattg catctatttt aaaagccgct gcccagtgga gcgataaagt
     2461 gatagtgatg gataatggta gtagtgatga aacctgggaa attgtacagc agttagcaac
     2521 agaatatccg caaataatac cctttgccaa agatgatcaa gcatttcaga taggattgag
     2581 agcggtaatg tttgatgctt ttaagcacga actaacagcc tctgattggt ggtgtattcg
     2641 cttagatgct gatgagtttt ttgtggagaa tccaagagat tttttggcaa ctattcccaa
     2701 aagatacaaa caagtatgca aagcaagtat tgattattgc cttacacacg aagatgtaga
     2761 agagtatacg tttacaggtg attttgaaca agataaacac aagattcgct attaccaacc
     2821 ttacacatgg gcagaactac gttttttgcg tcatagcaaa aaattgcagt gggaaacagg
     2881 cgaacgcaga ccagaacctt gtggattaac ttatccgaaa caaattaagg tgttgcatta
     2941 tcagttccgt agtccgcaac agatgcagca acgctatgct gtgcgtcagc aagccaaagc
     3001 caatggttgc ggttcttttc gccacgaaaa agggaacgat tggaaagatt atttgagaaa
     3061 tagaaatgaa ctagtgtacg ataagcaaga tggaacgtac caaaccttgg gcaatcgtaa
     3121 taagtttaat aaatggcata cccgtattac caaaggtttt ttaacgctgt ttggttatta
     3181 ttgagcgatt cactttttgg gactgttagc aatagcgtta taaaaaaacc aaatataata
     3241 gcaaattgcc ctgttatgaa tgtgtcgaac agtgattgcc aagaaaataa gaacaatccc
     3301 aacagcaaca aagtcattct gctacgatta accagcctaa tagggtagta ataaatggct
     3361 aatagcaaga caagtccaat caagccaaat tcgatggtag tttgcaaaaa ctgattgtgt
     3421 acatctacac gtttatttgt tttccataat tcatataaaa ttttagattg ttttccttgt
     3481 cgaacactgt caatttgtgt ctgtccatcg cttaatccat gccccaatat cggtttttct
     3541 tgtatgatgg gtagggcaac ttctgtccat aaaaataagc gtggctcttg ctcaatcatt
     3601 ttttctgaca tacgttcgtg ttgcaacacg gcaaacacca tgccaataag aataaaaacg
     3661 aaagctccaa ttgccactat tttttttgtt aaccaaattt ttcgaataac aatgatagcg
     3721 gataaaccaa tagcagcgat aaatccagaa cgaccctccg acattaataa ggtgcttata
     3781 ataagcaaaa tactaaaccc atatatagtt ttcacactcc aatggagttc tttaaagcgt
     3841 gttacagcca aataccagac actagccaat gcaatattta agtataaatt aaaagccata
     3901 tgcgagttaa cgaatgcaat gcgagcttgc gtaaataaat catacctatt ttcgtttgtc
     3961 aaaaattcgg gcaagccaat gtggtaaata ataagataaa ggataagcaa ggtagatgaa
     4021 ataatgatag tatgtaaaaa ataggataat ttatacaacg aattatatcc aaaaatacct
     4081 attatagcaa atccaaataa ggctaatcga cgttctgtta ggttatgaaa ataggtatgc
     4141 gaatcttcga atggataata taaaaaaccc attgtaaaaa acaacaatat accaataaaa
     4201 taccaggtgg tagctgacaa ttttatgtgt ttccatttca tgtcaacaaa aaattctacg
     4261 atataggata taaaaaacaa cgtaagggca agctgctgtt tcggataata aaatggcaac
     4321 gaagccatta aaaaaagacc acaaagcaga ttgatactat gtacgtaatg cctaaatttt
     4381 tctaacataa gttgcgttat ttgaataaac gggaaggatt gttgaataat cgtgcgtaaa
     4441 ataatttaaa cccaaaccat gtaagtactt tctgattatc ggtaatagtg gcaaaataat
     4501 caagttcttg ttttatttcc gagtaagtaa aattacgaat acctaatttg cgaatcaatt
     4561 tctttaattt ctttttttct atcaactttg tttctgcttc tgataggtgt gtgcaagaaa
     4621 atggaagttg gtttatccat tttttataaa caaggagcaa atttttgtaa taatccgtat
     4681 tgtgagagcc tgcagaaaaa tgctctagta aaatatcgta aacaacataa tttcttaaat
     4741 tattggcttt agcttgcata caaatatcgt aatcatatcc atgaaagccc gaaatttttt
     4801 catcaaattg caaaaattga aagttttctt ttgtagaagc taaaaagaca ccatctaaca
     4861 gaacaacttc ttcacgggtt gattcgggtt ggtttggagt acgaatataa ctagattttc
     4921 ctttgcctga ttgaattagg ttcatcttta aaccatatgt tgaccatgac gctggtatgc
     4981 gcgaagctaa acaaccacct gctaagccta ttaagccaac ttgtggcgtt tgtaaatgtt
     5041 ctatgatgtt aagtccccaa tcagagcttc taaaaaaaac atcttcgtgt acaaaacata
     5101 agtaaggaaa tttgcttttt gttactccca gattgtatgc a
//
LOCUS       DAOSMY010000077         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_16406_length_5126_cov_3.980477, whole genome shotgun sequence.
ACCESSION   DAOSMY010000077 DAOSMY010000000
VERSION     DAOSMY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16406_length_5126_cov_3.980477"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLD76_02790"
     CDS             complement(<1..886)
                     /locus_tag="PLD76_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU26963.1"
                     /translation="MTEQVIVLEHADPTIFCGVNNAHILVIKNLFPKVRIIARGNAIK
                     AMGDKDEIALFAEKITKIEQHITEYNNLSEEVIINIIKGETPLVCKPNNLILHGLNGK
                     PITARTENQQKLVELFDTHDMLFAEGPAGSGKTYTAIALAVRALKNKTAKKIILSRPA
                     VEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMIPAVKLKDYIENNIIQIAPLAFMRGR
                     TLNDAIIILDEAQNTSTQQIKMFLTRLGLHSKMIITGDITQIDLPSSQVSGMIHAMKI
                     LRHVEGVAHIHFTKQDIVR"
     gene            1264..2241
                     /locus_tag="PLD76_02795"
     CDS             1264..2241
                     /locus_tag="PLD76_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU26964.1"
                     /translation="MNKCMEKPYVVGIDIGGTNTVFGIVDARGSVLATNSIKTQKHTV
                     LEDYIQELYDELTKLIADNTHISKIKGIGIGAPNANYFNGTIEQAPNLPWKGIIPFAD
                     KIQKKFGLPVAITNDANAAAIGEMIYGAARGMKNFIMITLGTGVGSGIVANGSLVYGS
                     DGFAGELGHTTMRRNNGRLCGCGLTGCLETYASATGVARSAREFLETQKDVPSRLRDI
                     PADEITSKDVYEAAVAGDKLAQEVFAFTGQILGEAFADFIKFSSPEAIIMFGGLSKAG
                     ELLLRPIRESMEKNVMPIFRNKVKLLFSELKEADAAVLGASALAWEIKD"
     gene            complement(2561..3469)
                     /locus_tag="PLD76_02800"
     CDS             complement(2561..3469)
                     /locus_tag="PLD76_02800"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="HOU26965.1"
                     /translation="MTLTDQVVKNIITRVIKSQDYRIEIVNLINAEFLQFAVEFFKKI
                     ANAKLNSEEITIDWYKKAFMDDSLSSDEIAINSGLNKKTISNMYGTATKTIVIEAANE
                     HFESLYNSIQSLVELEKEIDLTLTIKMKGVSVDLNVSESLIVINTLAVKRAALRGGLW
                     STAGKSAEKYLMLTLCKMYQVEEKYYDASHFVKDKGKKVDREIDFYLLNNGKEYLCEV
                     KLMGKGNPESADAIIARDTNVFVADTLSLQNKNQCEQLNINWIALRDENGFKRFKIAL
                     EKLGIPHVDYLGDLEIDLPKILDSLF"
     gene            complement(3466..4845)
                     /locus_tag="PLD76_02805"
     CDS             complement(3466..4845)
                     /locus_tag="PLD76_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU26966.1"
                     /translation="MITKAYITELKHTGNGDIAKLIKSQRDVGTINNILESLGQLPAN
                     FESDFLYELLDHQHAQVRLNAVKNIGKLNGKSKTIKLHELYERETDTSVRREIISSIG
                     RQRKPENKIFLFETLHDDDPKIVCQAIRALLVFEKDIDVQEHLKPLINHTNEMVRNVI
                     YKEYFAKDNQKNGVLPHTETYDFLKNTVVNADVLEALKFVPDESVHLTFTSPPYYNAR
                     DYSIYPSYEAYLEFLENVFKETHRITKEGRFLIVNTSPIIIPRVSRSHSSKRYGIPFD
                     LHPLLVKNGWEFLDDIIWLKPEASVKNRIGGFMQHRKPLGYKPNCVTEYLMVYRKQTE
                     KLLDWNIRSYDYQTVEESKVADGYETTNVWKIDPTFDKVHSAIFPVELCKRVIQYYSY
                     KGDLIFDPFGGSGTVGKTAKSLNRSFFLTEQEPTYFEYMKAKAKPGALFKERETRFLT
                     LEQFKNIAK"
BASE COUNT         1522 a          915 c          899 g         1790 t
ORIGIN      
        1 gtcgtacaat atcttgtttg gtaaaatgaa tatgagctac accttctacg tggcgtaaaa
       61 ttttcatagc gtgtatcatc ccagacactt gcgatgaggg taagtcaatt tgcgtaatat
      121 caccagtgat aatcatttta gagtgcaaac ctaagcgggt aagaaacatt ttgatttgtt
      181 gtgtgctcgt attttgagct tcgtccaaaa taataatggc atcgttgagt gtgcgtccac
      241 gcataaaagc caacggagct atttgaatga tgttgttttc gatgtaatcc tttaatttta
      301 ctgcaggaat catatcttgc aaggcatcgt acagaggttg taagtaaggg tcaatttttt
      361 ctttcatatc tccaggcaag aaacccaact tttcgcccgc ttcaacagct ggtctgctta
      421 aaataatttt ttttgctgtt ttatttttca acgctctcac tgctaaagca atggctgtat
      481 acgtttttcc cgatccggca ggtccttcgg caaatagcat atcgtgtgta tcaaataatt
      541 ctaccaactt ttgctgattt tcagtgcgag ctgttattgg ttttccattc aacccatgca
      601 aaattaaatt atttggcttg catacaaggg gtgtctctcc tttgatgata ttaataataa
      661 cttcttctga taagttgttg tattcggtaa tatgctgttc tatttttgta attttttcgg
      721 caaacagagc tatttcgtct ttatcgccca ttgccttaat agcatttcct cgtgctataa
      781 tacgtacctt tggaaacaga ttttttatta ctaaaatatg agcattattg acaccacaaa
      841 agatagtggg gtcagcatgt tccaacacga ttacttgttc tgtcattggt aaaattttgg
      901 tatgcaaagg tacatatttt cttttatatt ttacagcaaa tttatcatta cttaattttt
      961 taagtaatat taggatagga aactttctat catagagaaa aaagataggg atataaaaca
     1021 agtaatgtga attctttctg ttttacacca taaaaatttt ctaccaatag catcatttat
     1081 caaaaaaata gtatttttgt agcaccaaag cccatcttta gctatagtaa gtgtgcaaaa
     1141 ggagacatta tttacttagt agtaatgagt cttatcaacc ttcacggttg ttaaggtatg
     1201 cttgatgagg cttttttgtt tttatagtaa acaaatagta aacacttgaa tattaacctt
     1261 aaaatgaata aatgtatgga aaagccttac gtagtaggta ttgatattgg cggaacaaac
     1321 acagtttttg gtatcgtaga tgcacgtgga agtgtattgg ctactaattc gattaaaaca
     1381 caaaaacaca ctgtgttaga agattatata caggaattgt atgatgagtt aactaaatta
     1441 attgccgata acacccatat ttctaaaatt aagggcatag gtattggagc tcccaatgca
     1501 aattatttta atggtactat cgaacaagcc cccaacttac catggaaagg tattattccg
     1561 tttgctgata aaattcaaaa gaaatttggc ttacctgtag ccatcaccaa cgatgccaat
     1621 gcagcagcta taggtgaaat gatttatgga gcagctcgtg gtatgaaaaa ctttattatg
     1681 attaccttag gcacaggagt agggagtggt atagtagcca atggctcgct tgtgtatggt
     1741 agcgatgggt ttgctggtga attaggacat accactatgc gtcgcaacaa cggacgtttg
     1801 tgcggttgtg gactaacagg atgcctagaa acctatgctt cggctacggg tgtggcacgt
     1861 tcggctcgcg aatttttaga aacacaaaaa gacgtaccaa gccgattgag agatattccc
     1921 gctgatgaaa ttacttccaa agatgtgtac gaagctgctg tagcaggaga taaactagca
     1981 caagaagtat ttgcctttac agggcagatt ttaggcgaag cttttgccga ttttattaaa
     2041 ttctctagcc ccgaagccat tattatgttt ggcggtttgt caaaagcagg agagttatta
     2101 ttgcgtccaa tacgcgaatc gatggagaaa aacgtaatgc caatttttag aaacaaagta
     2161 aaattattgt tctccgaact caaagaagca gatgctgccg tattaggtgc cagtgcctta
     2221 gcttgggaaa taaaagatta atacgcacgt ttcaacacta tttacaaata agcctaagct
     2281 aatgcttagg cttatttttt atacttgtag agtattgttg ggtattggtg ttagtggatt
     2341 tctttctccg taatcctaaa ataccttgtt tttttgtttc cattttacac ttttttgtgt
     2401 aaacctattt caggacaaga cagtcgcact gcgacaaagt gaattgaaaa tcagcgtagc
     2461 taatgcggga gcaaatgccg ctaaatgcac gtcagccgcc aatgcgcata ggcgtgtgtt
     2521 atgcccagtt tttttgtctt tcaaaatgaa attatgtctt tcaaaataga ctatctaaaa
     2581 ttttgggtag gtctatttct aaatcgccta aataatcaac atgaggtata cctaattttt
     2641 caagagctat tttaaatcgt ttgaaaccat tttcatcacg taatgctatc caatttatat
     2701 ttagttgttc acattgattc ttgttttgaa gtgaaagtgt gtcggcaaca aaaacatttg
     2761 tatctcttgc aataattgca tcggcgcttt caggatttcc ttttcccatt aatttaactt
     2821 cacacaaata ctctttccca ttattaagca aataaaagtc tatttctctg tcaactttct
     2881 ttcctttatc cttaacaaaa tggctagcat cataatattt ttcttctact tgatacattt
     2941 tacatagagt aagcattaga tatttttcag cactttttcc tgctgtgctc cataagccac
     3001 ctcttaatgc agcgcgttta accgctaaag tattaatgac aatcagactt tcactaacat
     3061 tcaaatcaac agaaacacct ttcattttta tggttaatgt caagtcaatt tccttttcca
     3121 attcgacaag cgactgaata ctattataaa gagattcaaa gtgttcattt gctgcttcaa
     3181 tgacaatagt ctttgttgca gtaccataca tgttgctgat tgtcttttta ttaagccctg
     3241 aatttatggc gatttcatcc gacgacaaag aatcgtccat aaacgctttt ttataccaat
     3301 caatcgtaat ctcttcagag tttaactttg catttgcaat ttttttgaaa aattcgaccg
     3361 caaattgaag aaactcagca tttatcaaat tcacaatttc gatacggtaa tcttgtgatt
     3421 tgattacacg tgtgattata ttttttacaa cttggtctgt tagtgtcatt ttgctatatt
     3481 tttaaattgt tctagtgtta gaaatctggt ttcacgttct ttaaaaagcg ctcctggttt
     3541 tgcttttgct ttcatgtatt caaaatatgt aggttcttgt tctgttaaga aaaaagacct
     3601 attcaatgat tttgcagttt tgcccactgt tccactacca ccaaatgggt caaatattaa
     3661 gtcaccctta tatgaataat attgtataac tcttttacat aactcaaccg ggaaaatagc
     3721 tgaatgaact ttgtcaaaag ttgggtcaat tttccaaaca tttgtagttt cgtaaccatc
     3781 tgctactttg ctttcctcta cggtttggta atcataactt cttatattcc aatctaaaag
     3841 tttttcggtt tgctttctat aaaccattaa atattcagta acgcaattag gtttataacc
     3901 aagcggtttt cggtgttgca taaatccgcc aattctgttt tttacacttg cttcaggttt
     3961 aagccagata atatcatcca aaaattccca tccatttttc actaaaagcg ggtgcaagtc
     4021 aaaaggtatt ccgtaacgtt tacttgaatg tgaacggctt actcgtggaa taatgattgg
     4081 cgaagtattt actattaaaa aacgtccttc tttagtaatt ctatgtgttt ctttgaatac
     4141 attctccaaa aattctaagt atgcctcata gcttggatat atcgaataat ctcttgcatt
     4201 gtaataagga ggcgaagtaa aagttaaatg aacactttca tctggaacaa actttaaggc
     4261 ttccaaaaca tctgcattta cgacagtatt cttaagaaaa tcataagtct cggtgtgcgg
     4321 aagcacaccg ttcttttgat tatcttttgc aaagtattcc ttataaatca catttctaac
     4381 catttcattt gtatgattaa ttaatggttt taaatgttct tgaacatcaa tatctttttc
     4441 aaaaaccaaa agtgctcgta tagcttgaca aactatttta gggtcatcat catgcaatgt
     4501 ttcaaataag aaaattttat tttcaggttt tcgctgtctc ccgattgaag aaatgatttc
     4561 tcgtctgaca cttgtgtcag tctctctttc gtataattcg tgaagtttta tagtttttga
     4621 cttgccgttt agttttccta tatttttaac agcatttagt cgaacttgag cgtgttggtg
     4681 gtcaagaagt tcatacaaaa aatcactctc aaaattagct ggtagttgcc ctaaactttc
     4741 aaggatatta ttaattgtcc cgacgtctcg ctgactctta ataagtttag caatgtcgcc
     4801 atttccagtg tgtttaagtt ctgttatgta agctttggtt atcatctttg tcttttttgt
     4861 tttgttgcaa aaatagtcat aatttgccta ttttcatcat ccttctcaac cgtcaaatga
     4921 cctttgtttt ctttttttta aaattgggca taacgttacg catatgccta gtggcggatt
     4981 gcggacgatt caactgtcaa accgtggtga agtttgagcg ggcgaaaagc cttgcaaagt
     5041 gcagtaaaaa ccgccattag gtatatgcga tgtgtgtgcc ctgcatgagc agagcgcacc
     5101 tgctgcaaag caacagaaaa aaatcc
//
LOCUS       DAOSMY010000078         5054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_16709_length_5054_cov_4.237848, whole genome shotgun sequence.
ACCESSION   DAOSMY010000078 DAOSMY010000000
VERSION     DAOSMY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16709_length_5054_cov_4.237848"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLD76_02810"
     CDS             complement(<1..634)
                     /locus_tag="PLD76_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOU26967.1"
                     /translation="MNTLRFKVLDEAFRRKAVQLPESKERLTDYYAKYVFDRNKMQRY
                     LSKNTYGILTNSIDKGEPLNRSIADGVATGMKQWAMEMGATHYTHWFQPLTGGTAEKH
                     DSFLDFNNIGGVIEDFSGKLLAQQEPDASSFPSGGIRNTFEARGYTAWDPSSPAFVVG
                     DTLCIPTIFISYTGEALDYKTPLLKSIGVLNKAAVDVCKYFDENVSKVTSF"
     gene            complement(654..1688)
                     /gene="asnA"
                     /locus_tag="PLD76_02815"
     CDS             complement(654..1688)
                     /gene="asnA"
                     /locus_tag="PLD76_02815"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOU26968.1"
                     /translation="MLLYIPKNYTPSLDSEKMELAIKQLKDDFQAELTHQLNLRRVTA
                     PLFVLSGTGVNDDLNGTERAVSFPIKDMGNRRAEVVHSLAKWKRMKLGAYNIPAGYGL
                     YTDMNAIRADEELDNLHSLYVDQWDWEQTITQEDRTIEFLKSTVKKIYQALKTIELKL
                     HGKYPHISPTLPSEIRFFHSEELLSLYPELSPRERETKVAEKYGAVFVIGIGAPLADG
                     KKHDGRAPDYDDWSTPTSKGLRGLNGDIILWNKVLDSTFEISSMGIRVDKKALLYQLK
                     EEGCMNRTELDFHSQLINEKIPLSIGGGIGQSRLCMYFLKCAHIGEVQASLWPEEMIE
                     SCKANKILLK"
     gene            complement(1779..2291)
                     /locus_tag="PLD76_02820"
     CDS             complement(1779..2291)
                     /locus_tag="PLD76_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26969.1"
                     /translation="MEPENDYVYINGFEETITSPSAFSIRISDRHFGIGSDGLVAVTA
                     GCDVVSGWGAVAIGLLAGILLVFSLRLIENVFKIDDPVGAASVHGITGAFGTLAVGLF
                     SQSTGLFYGFGTQALLSQFIGVVSVAAWGLGTGFILFFILKKTVGLRIDKRVEEEGLD
                     IYEHGETAYN"
     gene            2769..4613
                     /gene="glmS"
                     /locus_tag="PLD76_02825"
     CDS             2769..4613
                     /gene="glmS"
                     /locus_tag="PLD76_02825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOU26970.1"
                     /translation="MCGIVGYIGKQDAYPILIKGLHRLEYRGYDSAGVALINEAGSLN
                     VYKSKGKVSDLEQSVTSKDISGTIGVAHTRWATHGEPNNENAHPHYSQSESLALIHNG
                     IIENYMVIKKELIRNGYTFKSSTDTEVLVQFIEYVKKTNQVDLCHAVQIALREVVGAY
                     AIAVLDKNDPKQIIAARKSSPLVVGIGDGEFFLASDATPIIEYTNKVVYVNDEEIAVI
                     HIDKGLKLLNLVDIEQTAEVKQLEMNLSQLEKGGYEHFMIKEINEQPHTLIDCIRGRI
                     NVEGTDVKLSGVIDHQDKFLKAKRIIIVACGTSWHAGLIGEYLIEDLCRIPVEVEYAS
                     EFRYRNPIIHPDDIVIAISQSGETADTLAAVEIAKSQGAFIYGVCNVVGSSIPRATHS
                     GSYIHVGPEIGVASTKAFTGQITVLSMLALTVAKLRKTIQPEEYRKLIGELQAIPNKM
                     QEVLKLDNQIKDLAKMFTYAHNFIYLGRGYNYPAALEGALKLKEISYIHAEGYPAAEM
                     KHGPIALIDAEMPVVAIATKDSIYDKTVSNIQEIKARKGRVIAVVTEGDDVVSEIADY
                     CIAIPESLEYFAPIVSAIPLQLFAYYIAVNKGRNVDQPRNLAKSVTVE"
     gene            complement(4706..>5054)
                     /locus_tag="PLD76_02830"
     CDS             complement(4706..>5054)
                     /locus_tag="PLD76_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOU26971.1"
                     /translation="LLPGGMPGTSNLNKHEKLKELISQHVHSGKLMGAICAAPLILGE
                     LNLLQGKIATCYPSFETYLHGATISDDKVADAGEIVTGKGPGFSMDFALTIVKKLKGA
                     CAADEVAKGLLLQ"
BASE COUNT         1670 a          840 c          897 g         1647 t
ORIGIN      
        1 aaaagctagt tactttgctc acgttctcat caaaatattt gcatacatca acggcagctt
       61 tgttcaatac gccgattgat tttagtagag gagttttgta gtcaagagct tcacctgtgt
      121 acgaaataaa aatagtaggg atacaaagtg tatcgccaac gacaaaagca ggagaagatg
      181 gatcccatgc agtataacct ctagcttcga aagtattacg gatacctcca cttgggaaac
      241 ttgaagcatc aggttcttgt tgtgctaata attttcctga gaaatcttcg attactccac
      301 ctatgttatt gaaatctaag aaagaatcgt gtttttcggc tgttccgcca gtaagaggtt
      361 gaaaccagtg agtataatga gttgcaccca tttccattgc ccattgtttc atacctgtag
      421 ctacaccatc agcaatgctt ctatttaatg gttcaccttt atctattgaa ttagttaata
      481 ttccataagt gttttttgaa aggtaacgtt gcatcttgtt acgatcaaac acatacttgg
      541 cataatagtc agttaaacgt tctttgcttt caggtaactg aacggctttg cgtctaaacg
      601 cttcatctaa tactttaaat cttagtgtgt tcatacgtgt aaaaaaatag ttattatttt
      661 aatagaattt tatttgcttt acatgattca atcatttctt cgggccataa tgatgcttgg
      721 acttcgccaa tatgtgcaca ttttagaaaa tacatacata atctagactg acctattcct
      781 cctccgatag ataaaggaat tttttcatta attagttgac tatgaaaatc taattccgtc
      841 cgattcatgc atccttcttc ttttagttga tacaagagtg cttttttgtc tacccgtatt
      901 cccatagagg atatttcaaa tgtactatct aatactttat tccacaaaat gatgtctcca
      961 tttaaccctc ttagtccttt gcttgttgga gtagaccaat cgtcgtaatc aggagctcgt
     1021 ccatcgtgtt tttttccatc tgctagtgga gctccaattc ctattacaaa aacagcacca
     1081 tatttttcag ccacttttgt ttctcgttct ctagggctaa gttctgggta caaagataat
     1141 aattcttctg aatgaaaaaa gcgtatttca ctaggtaatg taggcgaaat atgtggatat
     1201 tttccgtgaa gtttcaattc aattgttttg agagcttgat aaatcttttt aactgtcgat
     1261 tttaagaatt caatagttct atcttcttgg gtaattgttt gttcccaatc ccattgatca
     1321 acgtataatg aatgtaaatt atccaattcc tcatcagctc taatggcatt catatctgta
     1381 taaagaccat atcctgcagg tatgttgtaa gcacctaatt tcatcctttt ccatttggct
     1441 aacgaatgta ctacttctgc acgtctgttg cccatatctt ttattggaaa tgatactgca
     1501 cgttctgttc cattaagatc atcattaact ccagttccag ataaaacaaa cagtggagcc
     1561 gtaacacgac gtaggtttag ttgatgcgta agttcagctt gaaaatcatc tttcaattgt
     1621 ttaatagcca attccatttt ttcagaatca aggctagggg tatagttttt tggaatataa
     1681 agtaacatag ttacatcaat ttagtttgcg ttttagaaat cattgaaaaa gagaggacta
     1741 aagttttttc aatagccccc tctttgataa aagtttaatt agttataagc agtttcacca
     1801 tgttcatata tatccaaacc ttcttcttct actcgtttgt caatacgtaa acctactgtt
     1861 ttctttaaga taaagaatag aataaatcct gtgcctaatc cccacgctgc tactgaaact
     1921 accccaataa attgtgataa tagagcttgt gttccaaatc catagaataa accagtagat
     1981 tgagaaaata agccaacagc taatgtgcca aatgcacctg taataccatg tacagaggct
     2041 gcaccaacgg ggtcatcaat tttgaatacg ttttcaatca aacgaagtga gaataccaat
     2101 aatatacccg ctaataatcc gatagctaca gcaccccatc cacttactac atcgcaacca
     2161 gcggttacag ctactaatcc gtctgaacca attccaaaat gacgatcgct aatacgtata
     2221 gaaaatgctg aaggagatgt tattgtttct tcaaaaccat ttatatatac gtagtcgttt
     2281 tccggctcca tgcatcttcg taaatttgat tagttttgac ataatatatt gtaaatcgat
     2341 taaataatga caaaatgttt tgttttcaat gcaaaagtat gaataatgat atttccagac
     2401 aagaaaaatg tgtataaatg ctatttttat tttcaataat atcaaaaaca aactaaatta
     2461 actataaaaa atttaatatg aaagcaaaat acgtttaaaa tgtcacataa tgaaattgtt
     2521 gttaaaataa aatgctaaaa ttaagtatgt aaaaaattct tttaatagga aaaaatatgt
     2581 tgaatatgat acaaaatgga tgataaattg gcaaattgat taaaatattt cgataataat
     2641 tacaaaaaga tttattttca cttaaatttt taaataaaaa actgcttaat atggaatagg
     2701 ataaggagaa aataaatatc tttgtagcaa aatatgttat ggtatacact ttttaaaata
     2761 acgaatttat gtgtggaatt gtaggatata tagggaagca agatgcttat ccaatactaa
     2821 ttaaaggctt acatcgtttg gaataccgtg gttatgatag tgctggtgtg gctttaatca
     2881 acgaagctgg tagcttaaat gtgtataaat cgaaaggaaa ggtatctgat ttagaacaat
     2941 cagttacttc aaaagatatt agtggtacta taggagtggc acatactcga tgggctacac
     3001 atggtgagcc taataatgaa aatgcccatc ctcattattc tcaatcagaa tcgctggcat
     3061 taattcataa tgggattatc gaaaattata tggtaatcaa aaaagagcta attcggaatg
     3121 gttatacatt taagagctct acagatactg aggtattggt acaatttata gaatatgtaa
     3181 aaaaaacgaa tcaagtagac ttgtgtcatg ctgtacaaat tgcgttgaga gaagtagtag
     3241 gtgcatatgc tattgctgtg cttgataaga atgatccaaa gcaaataatt gctgctcgca
     3301 aaagtagtcc attagttgta gggattgggg atggcgagtt ttttttagcc tctgatgcta
     3361 ctccaattat tgaatatacg aataaagtag tgtatgtgaa tgacgaagaa atagccgtga
     3421 tacatataga taagggttta aagttgctca atttagttga tattgaacaa acagctgaag
     3481 taaaacagtt ggaaatgaat cttagtcaac ttgaaaaggg tgggtatgag cattttatga
     3541 ttaaggaaat caacgaacaa ccacacacct taatagattg tatcagagga cgaattaatg
     3601 ttgagggtac tgatgtaaaa ttgtctggtg tgattgatca tcaagataaa tttttgaaag
     3661 ctaaacgtat tattattgta gcatgtggta cttcttggca tgcaggatta ataggcgaat
     3721 atttgataga agatttgtgt cgtatacccg tagaggttga atatgcatcg gaatttaggt
     3781 atcgcaatcc aattattcat ccagatgata ttgtaatagc catttctcaa tcaggagaaa
     3841 ctgccgacac cttagctgct gtagaaattg ctaaaagtca aggagcattt atttatggtg
     3901 tctgcaatgt ggtaggatcg tctatacctc gtgcaacaca ttcgggatcg tatatacatg
     3961 taggtccaga aattggtgtt gcatctacta aggcatttac tggacaaata actgtattgt
     4021 caatgttggc gttgactgta gccaaattgc gtaaaactat tcaaccagaa gagtacagaa
     4081 aacttattgg agaattgcaa gctattccaa ataaaatgca agaagtactc aaactagata
     4141 accaaataaa agatttggca aaaatgttta cgtatgccca taattttata tatttgggtc
     4201 gaggctataa ttatccagca gcccttgagg gagcattaaa actaaaagaa atatcatata
     4261 tccatgccga agggtatcct gctgcagaaa tgaaacatgg tcctatagcg ttgattgatg
     4321 cagaaatgcc agtagtggcg atagctacta aagatagtat ttatgataaa actgttagca
     4381 atattcaaga aataaaagcc cgtaaaggaa gggttattgc cgtggttaca gaaggagacg
     4441 atgtggttag cgagattgct gattactgta ttgctatacc agaatcatta gaatattttg
     4501 ctcctattgt ttcggctatt ccattgcagt tatttgctta ttatatagct gtaaataagg
     4561 gtcgcaatgt agaccaacca cgtaatttag ccaaatcggt aacggtagag taaaagctat
     4621 ttattttgtt tatattaaaa agaatcgctc caaaataggg cgattctttt gttagataga
     4681 ggttaaaaat caatgtgtaa cgaaactatt gcaacaacaa tcccttagct acttcgtcag
     4741 cagcacaagc cccttttagt ttttttacta ttgttagagc aaaatccata ctaaatcctg
     4801 gaccttttcc tgtaactatt tcgccagcat ctgctacttt atcatcactt atagtagctc
     4861 cgtgtaaata tgtttcaaaa cttgggtaac aagtggcaat ttttccttgt aataaattca
     4921 actcgcccaa aataagtggt gcagcacaaa tggctcccat taatttacca ctatgtacat
     4981 gctggctgat taattctttt agtttttcat gtttatttaa gttcgaagtg ccaggcatac
     5041 ctcctggtaa cagt
//
LOCUS       DAOSMY010000079         4985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17000_length_4985_cov_3.009939, whole genome shotgun sequence.
ACCESSION   DAOSMY010000079 DAOSMY010000000
VERSION     DAOSMY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17000_length_4985_cov_3.009939"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLD76_02835"
     CDS             complement(<1..310)
                     /locus_tag="PLD76_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU26972.1"
                     /translation="MSLFVKIIVDNHSQQDNFLTEHGICVYVETLTHKCLVDVGASDK
                     FIRNAEQMGIDIAQIDYLFLSHGHVDHVGGLPFFLKKNAKAKIIFSDKLFTQQYFSKR
                     H"
     gene            complement(307..717)
                     /locus_tag="PLD76_02840"
     CDS             complement(307..717)
                     /locus_tag="PLD76_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOU26973.1"
                     /translation="MPRPQQHRTLLTPPKMKGFKPFGIPTKSLSSVVLLYDEYESIRL
                     LDYEGLTQEQAAERMGVSRPTLTRIYGKARQTMAQAMVEGKMILIEGGNVSFNSQWYR
                     CKKCYKLIQGKENHLHCDNCPCYGDDELEKIGKE"
     gene            complement(720..1079)
                     /locus_tag="PLD76_02845"
     CDS             complement(720..1079)
                     /locus_tag="PLD76_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOU26974.1"
                     /translation="MKKTLVIGASENPERYAYKAITALVAHGHEVKALGLKKGNVAGI
                     EFDTEQLPYQGIDTVSLYVGPQNQPSYYEFIEKLQPKRVLFNPGTENSEFQARLIAKG
                     IEVEEACTLVLLSTNQY"
     gene            complement(1275..>2987)
                     /locus_tag="PLD76_02850"
     CDS             complement(1275..>2987)
                     /locus_tag="PLD76_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOU26975.1"
                     /translation="YCQTIATKADTEKRFLVVLGSRPYHTDPLVQHKIADVIADFGVD
                     VITEDIARMAAKKLPKVHMVMQWAYTNRILKAATWVANKGEHVQYMQLTSFGCGPDAF
                     IIDEVNTLLQQVSKNPTILKIDDINNIGSTRLRIRSVIESLGLSSQAVQSTSTENSSV
                     PFFEKKDRKRKILLPWFADFYSPFIPPIFHRLGYEAENLPPSDMESVAYGLKYSNNEI
                     CYPATLVVGDCMKALASGKYKREEIALGISQTGGQCRATNYISLMKKAMLSAGYDDIP
                     VISVGVGGTGVLNEQPGFEVKWHKVLKLIIATMGYADCLSQLYYSTAPRERVQGAAER
                     LKNKYISLAVEAVEKHQTRVLNELLVQAVKEFSDSNSFRDIPRMGIVGEIYVKYNNFG
                     HKNITHWLISQGIEPVLPPLSAFFTETFASKKARIVGNIEHKNNKQFIMNWAERYVFS
                     ILHKMERCMVDYPYHIPLTNPHHDALKASKIINLNTQFGEGWRIPAEFAHFAEHGINH
                     VISLQPFGCIANHIISKGIEKRTRELYPKLQLLQLDFDSSMGEANIFNRLHFMIRNAQ
                     KEKM"
     assembly_gap    2988..2997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2998..>4985)
                     /locus_tag="PLD76_02855"
     CDS             complement(<2998..>4985)
                     /locus_tag="PLD76_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOU26976.1"
                     /translation="VSKQDIALSIFHAVSLQVISSLSKGNTIEAPLFLSGGPFAFIPT
                     LKKVFMQQLSINEAQIIDSENRQFIPAWGAAIESNQQTSTLALSALIKKIEQSKQQVY
                     TYNPTALPPLFSDNEHYLRWKKESSCVIETIDIADATEGCYIGIDSGSTTTKIVALDE
                     KKRVFYRFYERNSGSSLDTVQKGLNSLLQQANEANKNLMVLGSCVTGYGEDLVKNAFP
                     IGLGMVETLAHFTASVHFNPHVSFILDIGGQDMKACFIENGAIKRLDINEACSSGCGS
                     FIETFAHSLGHTAASFSDLCLQSKRPYDLGTRCTVFMNSKVKQALREGAPMADIAAGL
                     GYAVIKNCLNKVLKINDFSTLGKHIMVQGGTFRNIAVLRSLEIETGSNVMITNMPELM
                     GAFGAALFALKNREKQQPTNLQDCVQIRTYTCQTLLCKGCENNCLVTRYQFGKESKFY
                     AGNKCEKVFSNRGNADIKGKNAFDYKYQLLFNRPHAKNPVLKLGIPRALGMYENYPFW
                     HGLFHACGIEVVLSDTSTLKLYEKGAHSVMADNICFPAKLTNGHIVNLMEKKPDRIFL
                     PFVVYENQSDTKAPNSYNCPIVTAYSEVIRSAINPMSSSGIPFDSPSVNFKDKKLLKQ
                     ACDNYLLSILPHLTKKDLKNAFEYAFKQQVIFEQTITD"
BASE COUNT         1471 a         1008 c          879 g         1617 t
ORIGIN      
        1 aatgacgttt agaaaaatat tgttgcgtaa agagcttgtc agaaaagatt atctttgctt
       61 tcgcgttctt tttcaagaaa aacggaagac caccaacatg atcgacatgt ccatgagaaa
      121 gaaacagata atcaatttga gcgatatcaa tgcccatttg ttcggcattg cgaataaact
      181 tatcagaggc accaacatcg actaaacatt tgtgtgtaag cgtttctaca tacacacaga
      241 tgccatgctc cgtaagaaag ttatcctgtt ggctatggtt atccactatt atttttacaa
      301 acagactcat tctttcccaa ttttttctaa ttcgtcgtcg ccataacatg gacaattatc
      361 gcaatgcaaa tgattttctt tgccttgtat gagtttgtag cattttttac atctatacca
      421 ttgcgaatta aaagaaacat tccctccctc aatcaaaatc atcttaccct ctaccatagc
      481 ttgtgccata gtttgacgag cttttccgta aatacgcgtt aatgtaggtc tagaaacccc
      541 cattctttct gccgcttgtt cttgggtgag tccttcgtaa tccaacaaac gaatagattc
      601 gtattcgtcg tacaacaaca ccacagaaga caatgattta gtggggatac caaagggttt
      661 aaaccccttc atttttggcg gggtcaataa ggttctatgt tgttgtggtc gtggcatact
      721 taatactgat tggttgaaag caataccaat gtacaagctt cttctacttc aattcctttg
      781 gctattaatc gtgcttgaaa ttcggaattt tcggtacctg gattaaacaa tacgcgtttt
      841 ggttgaagtt tttcaataaa ttcgtaataa cttggttggt tttgtggacc aacatacaag
      901 cttactgtat ctatgccttg ataaggtagc tgctcggtat cgaactcaat accagctacg
      961 tttccttttt ttagtcctaa ggcttttacc tcatgaccat gtgctactaa agccgtaatg
     1021 gctttgtatg cgtatctttc tggattttct gatgctccaa ttacgagtgt ttttttcata
     1081 ttactatttg tagttttgtt acacaattgt tttaattgaa gtacaaatat aatgaacatt
     1141 tgttcattat aaaaataaaa tagtaaaatt taacgatttt ttgtagtaca acaaaaattt
     1201 accctttata cactaattaa atttactttt ccatatttaa ttagtgtata aaggactata
     1261 cgatagaaaa gaacctacat tttctctttc tgcgcattac gtatcataaa atgcaagcgg
     1321 ttaaaaatat tggcttcgcc catgctactg tcgaaatcga gttgcaataa ttgtagttta
     1381 ggatacaatt cgcgggtacg tttttcgata cctttggaaa tgatgtgatt ggcaatgcat
     1441 ccaaaaggtt gcaaactaat aacatggttg ataccgtgct ctgcaaaatg agcaaattcg
     1501 gcaggaatac gccatccttc gccaaattgg gtattcaaat tgataatttt cgaagctttt
     1561 agggcatcgt gatgaggatt agttaatggg atatggtatg gataatcaac catacaacgt
     1621 tccattttat gcaaaatact aaacacgtat ctttcagccc aattcattat gaattgcttg
     1681 ttgtttttat gctcaatatt tcctacgata cgagcttttt tgctagcaaa ggtttcggta
     1741 aagaaagcac ttaatggagg caatacaggt tcaatccctt gactaataag ccaatgtgta
     1801 atgtttttat gaccaaagtt attgtattta acataaatct cacctacaat acccatgcgt
     1861 ggaatatccc taaaagagtt gctatctgaa aattctttta ctgcttgtac caacaactca
     1921 ttcaacaccc tagtttgatg tttctccact gcctctacgg ctaatgaaat gtatttgttt
     1981 ttaaggcgtt cagctgctcc ttgcacgcgt tctctgggag ctgttgaata gtataattgc
     2041 gataagcaat cggcataccc catggttgca ataatcaact tgagcacttt gtgccattta
     2101 acttcaaaac caggttgctc gttgagcacc cccgtacctc caacgccaac agatataact
     2161 ggaatatcat catatccagc tgataacatg gcttttttca ttagcgaaat atagttggtt
     2221 gccctacact gaccacctgt ttgtgatatc cccaaggcaa tttcttccct tttgtatttg
     2281 ccactagcca aagccttcat gcaatcgccc acaaccaagg tagcaggata acaaatttcg
     2341 ttgttggaat attttaatcc atacgcaacc gactccatat cgcttggcgg taggttttct
     2401 gcctcgtagc ctaagcgatg aaaaatgggc ggaataaatg gcgaataaaa atcggcaaac
     2461 caagggagta agattttacg ttttctatct ttcttttcaa aaaatggaac acttgagttt
     2521 tcagtacttg tgctttgaac agcttgactt gacagaccca aactttctat cacagagcga
     2581 atacgcaaac gcgtagaacc gatattgttg atatcatcaa ttttcagaat agttgggttt
     2641 ttgcttactt gttgcaataa tgtatttacc tcatcaatga taaacgcatc gggtccgcaa
     2701 ccaaaagatg taagttgcat atactgcaca tgttcacctt tattggctac ccaggttgca
     2761 gcttttaata ttctattcgt ataagcccat tgcattacca tgtgcacttt gggcagtttt
     2821 ttggctgcca tccttgcaat atcttctgta atcacatcca ctccaaaatc ggcaatcaca
     2881 tcagcaattt tgtgctgcac caatgggtcg gtatggtagg gacgcgagcc taataccaca
     2941 agaaaacgct tctcagtatc agcttttgtt gctattgttt ggcaatannn nnnnnnnaat
     3001 cagtaatggt ttgttcaaaa attacttgtt gcttaaacgc atattcaaag gcgtttttca
     3061 agtctttttt tgtaagatga ggcaatatcg acaataagta gttatcacaa gcttgtttca
     3121 gcagtttttt atccttaaaa tttacacttg gcgaatcaaa tggaataccg ctactagaca
     3181 taggattgat agcactacgt atgacctccg aataggctgt tacaatgggg caattgtagc
     3241 tgtttggagc tttcgtatcc gattggtttt cgtacaccac aaatggcaag aaaatacgat
     3301 caggcttttt ctccatcaag ttaacgatat gtccgttggt tagctttgca gggaaacaaa
     3361 tattatcagc cattactgag tgagcccctt tttcatataa ttttaaggta gatgtatccg
     3421 acaataccac ctcaatacca caggcatgaa acaagccatg ccaaaaagga taattttcgt
     3481 acatacccaa ggcacgcgga atacctagtt tcaacacagg atttttagca tgaggacgat
     3541 taaacaaaag ttggtattta taatcaaacg cattcttccc ttttatatca gcattacctc
     3601 tgtttgaaaa cactttttcg catttatttc cagcatagaa cttactttct ttgccaaatt
     3661 gataccgagt aaccaaacaa ttattttcgc accctttaca cagtaatgtt tgacaggtat
     3721 aggtgcgaat ttgtacgcaa tcttgtaagt tcgttggttg ttgtttttct ctgtttttca
     3781 gagcaaacaa agctgcacca aatgccccca ttaattcagg catattggta atcatcacat
     3841 tgctgccagt ttctatttct agcgaacgca atacggcgat atttctaaac gtgccacctt
     3901 gcaccataat gtgtttgcca agtgttgaga aatcattgat ttttaacacc ttgtttaggc
     3961 agtttttaat aacagcatat cctaacccag cggctatgtc agccattgga gcaccctcgc
     4021 gtaaagcttg tttcacttta gagttcataa aaacggtaca ccgtgtaccc aaatcataag
     4081 ggcgtttgga ttgcaagcac aagtcagaaa aagaggcagc ggtatgtccc aacgaatgtg
     4141 caaatgtttc gataaacgaa ccacaccccg aagaacaagc ctcattgatg tccaaacgtt
     4201 ttatggcacc attttctata aaacaggctt tcatgtcttg cccaccaata tctaaaataa
     4261 aacttacgtg gggattaaaa tgtactgaag ctgtaaaatg agccaaggtt tctaccattc
     4321 ccaatccgat gggaaaagca ttttttacta aatcttcgcc atagcctgtt acacaacttc
     4381 ccaagaccat taaattttta ttggcttcgt tggcttgttg caacaatgag tttaagcctt
     4441 tttgtaccgt atccaacgaa cttccactat tgcgttcgta aaaccgataa aatacccgtt
     4501 ttttttcatc cagggcaaca atcttggttg tggttgaacc gctatcaatt ccaatataac
     4561 acccttcagt tgcatcggct atatcaatgg tttcaattac acaactactt tcttttttcc
     4621 acctcaaata atgctcatta tctgaaaata aaggaggtag tgctgttgga ttgtaagtat
     4681 aaacttgttg tttcgattgt tcaatttttt ttattaatgc agataatgcg agtgtggatg
     4741 tttgttgatt tgattcaatt gcagcacccc atgcaggtat gaattgcctg ttttctgaat
     4801 caattatttg tgcctcattg attgacaact gttgcataaa cacctttttc aaggtaggaa
     4861 taaaagcaaa cggaccacca gataaaaata acggagcctc tatggtattt cctttggata
     4921 aagacgatat tacttgcaac gataccgcat gaaatatgga taaggcaata tcttgtttgc
     4981 tcaca
//
LOCUS       DAOSMY010000080         4934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17225_length_4934_cov_3.497643, whole genome shotgun sequence.
ACCESSION   DAOSMY010000080 DAOSMY010000000
VERSION     DAOSMY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17225_length_4934_cov_3.497643"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLD76_02860"
     CDS             complement(<1..346)
                     /locus_tag="PLD76_02860"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOU26977.1"
                     /translation="MKHYLICLILLLSIGSVIQAADNKGITLTVSVSELRNSNGNVVF
                     ALYNRKDAFPDEHYKKYYKIVRGKIVNSSSVVVFKDLPIGEYAVNILHDENNDGKIKK
                     KFIPDSAIEKNHY"
     gene            complement(561..773)
                     /locus_tag="PLD76_02865"
     CDS             complement(561..773)
                     /locus_tag="PLD76_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26978.1"
                     /translation="MSNILVSGLINIETTLQCDQFPIDYSPVRYPFFGIQNTISGVGY
                     NVAKALTLLSDNVQFMSIIGKDKEGE"
     gene            complement(808..1386)
                     /locus_tag="PLD76_02870"
                     /pseudo
     CDS             complement(808..1386)
                     /locus_tag="PLD76_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(1396..3813)
                     /gene="lon"
                     /locus_tag="PLD76_02875"
     CDS             complement(1396..3813)
                     /gene="lon"
                     /locus_tag="PLD76_02875"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU26979.1"
                     /translation="MKDNPNKLFIGTNLQNDDSDFIPLIGDEDDDHVVDLSEDKVLPI
                     LALRNMVLFPGVVIPVTIGRPKSLKLIKDVNKRNGFLGTIAQKDNKIEEPELNDLYAV
                     GTVAQVVRVLEMPDNSTTVILQGRQRFAIQELDATEPYLKAKVELLADSEVPKHKDFK
                     ALMEAIKDLSLRMIKISSNMPQEIMFAIRNIENPRFFINFISSNLGLSLIDKQSLLEI
                     NNIEQRAMMLLKILTKESQLLEMKASIQSQAMENIDKQQREYFLQQQMKTIQKELGGN
                     VQDDLINDIKKKAEGKKWDEKVRKIAEKEIAKLETTSTHSSDYSVQLNYVQTLVDLPW
                     NEYTKDNFNLKRAQRVLNRDHFGLDEVKDRIIDHLAVLKLKGDLKSPIICLYGPPGVG
                     KTSLGKSIADALGRKYARISLGGLHDEAEIRGHRRTYIGAMPGRVIQNILKTNSSNPV
                     LVLDEIDKVGRDYKGDPGSALLEVLDPEQNFAFHDNYLDVDYDLSKVLFIATANSLSS
                     ISQPLLDRMELINVTGYIVEEKIEIAMKHLIPKQLENHGIPKDAVSFSKKTVQEIVEK
                     YTRESGVRNLEKQIAKICRRLARKIAMEEKYDTSMPISDLKEYLGTEKYTRDKQSQKE
                     EIGIVTGLAWTSVGGEILEIETSLSKGKGGKLTLTGNLGDVMKESAMLGLEYIRAHAK
                     DFGLNEDLFEETNVHIHVPEGAIPKDGPSAGITMATSLLSAFTKRKVKKHIAMTGEIT
                     LHGKVLPVGGIKEKILAAKRAGITDIILSHENKKNIDEINAIYIKGVTFHYVKDIKEV
                     IQIALLK"
     gene            complement(3962..4930)
                     /gene="pheS"
                     /locus_tag="PLD76_02880"
     CDS             complement(3962..4930)
                     /gene="pheS"
                     /locus_tag="PLD76_02880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU26980.1"
                     /translation="MSKEEVEELRIKYLSKKGEISTLFNDFRNVANEEKKVVGQLLND
                     LKNKAQDKINELKTTFDNQQNNTNDLDLTRSSSPIELGTRHPLSIVKNEIVDIFARIG
                     FTISEGPEIEDDWHVFSALNFPPEHPARDMQDTFFIEKNPDVLLRTHTSSVQTRVMKQ
                     AQPPFRVLCPGRVFRNEAISYRAHCFFHQVEGFYVAENVSFADLKQALLYFAKEMFGE
                     HTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCPFCKHTGWVEILGCGMIDPNVLESCG
                     IDSKKYTGYAFGMGIERITNLKFQVKDLRMFSENDVRFLKQFEAAL"
BASE COUNT         1386 a          968 c          862 g         1718 t
ORIGIN      
        1 aataatgatt tttttctatt gcggaatctg ggataaactt ctttttaatt ttaccatcat
       61 tattttcatc gtgtaaaata tttacagcat attccccaat tggcaaatct ttaaatacaa
      121 ctaccgagga gctattgaca attttgcctc tcacaatttt ataatacttt ttgtaatgct
      181 catccggaaa tgcatctttt ctattgtaca aggcaaaaac tacattcccg ttggaatttc
      241 tcaactcact cacacttact gtgagtgtaa ttcctttatt atctgctgct tgaataacag
      301 agccgataga taaaagcaga attaagcaaa ttaaataatg cttcataaat gatatatttt
      361 ttttgataaa catcttattc tgctatcttt ttcgattttt tattcattct ttcttttatt
      421 gtatctacta cgtaatacac cattggcaca aggaatactg taagaattaa cgaagataat
      481 aatccgccga taggtgtttg agcgatgctg ggtgtaatgt attgtgtgtc aatgtgtagc
      541 gattgtaatt cgtcggttat tcattcgcct tctttgtctt ttccgataat agacataaac
      601 tgaacattgt cggatagcag ggtaagagct ttagctacat tgtagcccac gcccgaaatg
      661 gtattttgaa taccaaaaaa tgggtaacgc actggcgaat agtcaatcgg aaactgatcg
      721 cattgcaagg tcgtttctat gtttattaaa ccagatacaa ggatattgct catgccttac
      781 ttcgatttgg ttactatatg taggtgctta ggataccttt ttgcctaaat tttcgacctc
      841 tgccaaccat tttttacgtg ttgcttctgt tgaggttttc acgctaccaa agttggtaat
      901 ttttacaggt ttgataccgc aaaactccaa aattcctttt ttcatggagt tatgtccggg
      961 ttgtttaaaa atccatttat agtaccaagt aggtgtatcc atggttacaa tcaatcgagc
     1021 cgttttacct accaataatt tatctcatag tggcgagttt tcgcggtacg agaatgcaaa
     1081 ttttggtaaa aatacacggt caataaaccc tttgagcaag gtagggtagg ttccccacca
     1141 agtaggatat acaaacacca gatggtcagc ctctttaatg gcttgttgca tcattaccaa
     1201 atcgggttct agttcggtgc gttgacggta gccaaatttt aggttcaaat caaattgcag
     1261 gtcgcttaaa tggactaagg tacaagctgc accactggcg tcagctcctt ttttgtacgc
     1321 agtagctaat tcaaagctca aactattttt gtcgggattg ccgttgatga ttaaaatctt
     1381 cttcatacat cccacttatt ttagtaaagc aatttgtatc acctctttaa tatcttttac
     1441 ataatgaaac gtaacccctt tgatataaat ggcattaatt tcatcaatat tctttttgtt
     1501 ttcgtgcgac aaaataatat ctgtaatacc agcacgtttg gcagccagaa ttttttcttt
     1561 aataccacct acaggcaaca cttttccatg taaggtaatt tctccagtca tggcaatatg
     1621 tttttttact tttcgtttgg taaaagcaga taacaagcta gtagccattg taataccagc
     1681 agaaggtcca tctttcggta tagcaccttc gggtacatga atatgtacgt tggtttcttc
     1741 aaataggtct tcgttaagtc caaaatcctt ggcatgagca cggatatatt ccaacccaag
     1801 catggcagat tctttcatca cgtcgcctaa attgcccgta agcgttagtt tgccaccttt
     1861 gcctttgcta agactagttt caatctctaa aatttcgcca cctactgatg tccaagccaa
     1921 accagtaaca ataccaattt cttctttctg actttgctta tctctggtat atttttctgt
     1981 tcctaagtat tctttaaggt cgctaattgg catagatgtg tcgtattttt cttccatggc
     2041 aattttgcga gccagtctgc gacaaatttt tgcaatttgt ttttctaaat tacgtacgcc
     2101 cgattcgcgt gtgtatttct ctacaatttc ttgtaccgtt tttttagaaa aagaaacagc
     2161 gtcttttgga ataccatgat tctctaattg tttaggaatc aggtgtttca ttgctatttc
     2221 aattttttct tctacaatat agccagttac gttaatcaac tccattctgt cgagcaatgg
     2281 ttgcgaaata ctactcaagc tattggctgt ggcaataaat aatactttag acaaatcgta
     2341 atctacatct aaataattat cgtggaaggc aaagttttgt tctgggtcga gcacttcgag
     2401 caaagcagag cctgggtcgc ctttgtaatc gcgaccaact ttatctattt cgtctaatac
     2461 caaaacagga ttagatgagt ttgtttttaa aatattttgg ataacacgtc caggcatagc
     2521 cccaatgtac gtacgacggt gtccacgaat ttcagcttca tcgtgcaatc cacccaacga
     2581 tatacgtgca tattttctgc ccaatgcatc agcaatggat tttcctaacg atgtttttcc
     2641 aacccctgga ggtccataca agcaaatgat aggcgatttc aaatctcctt ttaatttaag
     2701 tacagccaaa tggtcaataa ttctgtcctt tacttcgtct aatccaaaat ggtctctatt
     2761 cagcacacgt tgggcacgtt tcaggttaaa attatctttg gtatattcat tccaaggcaa
     2821 atcgactaag gtttgcacat aattcagttg taccgaataa tcgctcgaat gggtgctagt
     2881 agtttctagt ttcgcaattt ccttttcggc aattttacgt actttttcat cccatttttt
     2941 tccttcggct ttcttcttaa tatcgtttat caagtcgtct tgcacattgc cacccaattc
     3001 tttttgaatg gttttcattt gttgctgcaa aaaatattcg cgttgttgct tgtcgatatt
     3061 ttccattgct tgcgactgga tagaggcttt catctcgagc agttgcgatt ctttggtcaa
     3121 gatttttaat agcatcattg cccgttgctc tatgttattg atttccaata agctttgttt
     3181 gtcaataagc gagagtccga gattagagct aataaagttg ataaaaaaac gaggattttc
     3241 tatgttgcgg atagcaaaca taatttcctg aggcatattg ctagatattt ttatcatgcg
     3301 tagcgatagg tcttttatcg cctccatcaa tgccttaaaa tccttgtgtt tggggacttc
     3361 cgaatcggct aaaagttcta ctttggcttt aagataaggt tcggtagcat ctaattcttg
     3421 aatggcaaaa cgctgacgac cttgtaaaat aaccgtggtt gaattatcgg gcatttctag
     3481 tactcgtact acctgagcca cagtacctac agcgtataag tcatttagtt caggctcttc
     3541 tatcttattg tctttttgag caatagtacc caaaaaacca ttccgtttgt ttacgtcttt
     3601 tatcagtttt agcgattttg gacgaccaat agtaacggga attactactc caggaaataa
     3661 aaccatatta cgcagagcca aaatgggcaa caccttgtct tcgctcaaat ctactacatg
     3721 atcgtcatct tcatcgccaa ttaagggaat aaaatcgcta tcatcgtttt gtaaatttgt
     3781 tcctataaaa agtttatttg gattatcttt catgttagtt tttgctatta ttggctaccc
     3841 atagccttta ttttttttga tgttttatct cttctctaaa ccaatacaat actatactaa
     3901 tcaaaatgca tgccaataca aatttctgcc aaaagagaaa agtatatgtc agaaaaaatg
     3961 cttatagagc tgcctcaaat tgttttagga aacgcacgtc attttcggag aacatccgta
     4021 aatctttcac ttgaaatttg aggttcgtaa ttcgttcaat acccatacca aaagcatatc
     4081 cagtgtattt tttactatca ataccacagc tttcgagtac atttgggtct atcataccac
     4141 atcctaaaat ttctacccaa ccagtgtgtt tgcaaaacgg acaacctttg cctccgcata
     4201 agttacagga tatatccatt tcggccgaag gttcggtaaa agggaaatac gatggacgaa
     4261 gacgaatttg tgtgtgttcg ccgaacattt ctttcgcaaa atacaataga gcttgtttta
     4321 aatcagcaaa agacacattt tcagccacat aaaatccttc tacttgatga aagaaacaat
     4381 gggcacggta cgaaatggct tcgttacgaa atacccttcc tggacacaat actctgaaag
     4441 gcggttgtgc ttgtttcatc acacgagttt gtaccgatga ggtatgcgta cgcaacaata
     4501 cgtcaggatt tttttcgata aagaatgtat cttgcatatc gcgtgcagga tgttcgggcg
     4561 gaaaattgag ggcagaaaat acatgccagt catcttcaat ttctggaccc tcagatatag
     4621 taaaaccgat acgtgcaaaa atatctacga tttcattttt aacgatagat aaaggatgac
     4681 gtgtacctaa ttcaatagga gatgaactcc gagttaggtc taggtcgttg gtgttatttt
     4741 gttggttatc aaatgttgtt ttaagctcat tgattttatc ttgtgcttta ttttttaggt
     4801 cgttgagtaa ttgtcccact actttttttt cttcgttggc aacgtttcta aaatcattga
     4861 ataaagtact tatttctcct tttttactta agtatttgat gcgaagttct tctacttcct
     4921 ctttgcttat aggg
//
LOCUS       DAOSMY010000081         4925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17259_length_4925_cov_3.427926, whole genome shotgun sequence.
ACCESSION   DAOSMY010000081 DAOSMY010000000
VERSION     DAOSMY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17259_length_4925_cov_3.427926"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4925)
                     /gene="sprA"
                     /locus_tag="PLD76_02885"
     CDS             complement(<1..>4925)
                     /gene="sprA"
                     /locus_tag="PLD76_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU26981.1"
                     /translation="YEGKEDDIIRKIEAGNVSMPLSGSLITGNTTLFGFKTEMQFGKL
                     NVTTVLSQQESESKTINLSGGAQTTDFDIAADQYDENRHFFLAHYFRDNYDSFMSKLP
                     HITSGIVINKIEVWITNKNAVDFNQARNIVGFMDLAEPTRINNTHWIPSATTLVPDND
                     ANSLYREVIGIDGYRTISLVNNALGQSLSGFGIVGGEDYVKIESARRLEPTEYSLNKQ
                     LGFISLRRTLGTDEVLAVAYEYTYNGKVYQVGEFSTDGIESPNVLTLKLLKGVSQSPN
                     VPMWDLMMKNVYSLGAFQVQKDKFRLDVMYRSDSTGVDLNYIPKGKIANTILLRVMNL
                     DRLDSRSQQNPDGVFDFVENFTVIPENGRIIFPVVEPFGSHLRKAFDDDVVANEFVFQ
                     ELYDSTIVVAKEVAEKNKFRLVGQYRSSSGSEIRLNAMNVPQGSVKVTAGGRQLTENT
                     DYTVDYMMGVVNILNQDIIESGTPISINLENKSLFSMQRKTLVGTHLDYAFSDKFNVG
                     GTFMHLSEKPLTQKVLMGNEPISNTIWGLNTSYTTQSQWLTNLLDKIPLLNLTEPSSI
                     NLNAEFAQMIPGHSKTIDEQGIAYLDDFESTKIAFDIRHPQMWGLASTPYHPTGGLFP
                     EAALVNDIEYGKNRALLAWYYIDPIFTRNTSRTPLHIKNDKEQLSNHFVREIREREIF
                     PNRQLLYGQSGYIQTLNVAYYPTERGPYNIYPTSYDSEGKLTNPKGKWGGMMRRLETT
                     DFENNNIEYMEFWLLDPFVYDTLHVMKGGDLYVNLGDISEDILKDGRKFFENGLPVTD
                     AQPQAETTNWGKVPSRQAVVYAFDNEANARKLQDVGLNGLTTNEEFTFGAYQTFINQL
                     KATLTPDALQKFENDPFSPLNDPAGDNFSHYRSSYYDNQQKGVLERYKRYNGTEGNSP
                     AQADTNEDFTTSATNLPDVEDINNDNTLNEYEKYYQYKVPLRPEEMVVGKNYIADVVT
                     STVELKNGKTEQVKWYQFKIPIRDAQNYTAIGGIRDFKSIRFMRMFLTDFEEDVMLRF
                     ATLQLVKGQWKTYTKELYSLDKTPTSTASISMSAVNIEENADKTPVNYVLPPGVSRVI
                     DPSQQQIRQDNEQALSMRILQLSPGDARAVYKTSGVDMRQYKRMQMFVHAEALADNTT
                     QLVDNEMRVFIRLGSDFRNNYYEYEIPLTLTPPGNYSTNKTSDRETVWPSDNMFDFPL
                     ELLTDLKIKRNQEKNRTGSVVSNTTPYYQYDPTKPSNKMTIVGNPNLGEVEVIMIGVR
                     NSSRDVKSAEVWVNELRLTEFNEEGGIAALANLGVNLSDFGRVNVSGRVETVGFGGIE
                     QNVMDRRIDDYYEYNVSSVFDMGRFFPQRARVRLPIAYSYAQQVSSPQYNPLDKDVLL
                     EEALDAAELKTQRDSIRDRSLDVVTNQSISVSNATIGIKSKTPMPYDPANVTVNYAYS
                     ETERQNPTTERDIEQNYRASLVYDYSWNPKPVEPFAESKLFAKPAWQLIRDINFYYAP
                     NKISFNTSMVRYYYEQKLRNLTDNSTPNLIPTSFQKEFMWNTNTDIRWDLTRSIKIAF
                     TSGNQARITENDGGVNKKLYPDEYEAWKDSVLTSILQLGNPYSYNQRINASWVLPFSK
                     IPSLNWITSNVKYTGSYNWEYGTVIDDQNLG"
BASE COUNT         1491 a          989 c          828 g         1617 t
ORIGIN      
        1 ttcctaagtt ttggtcatca attactgtac cgtattccca attataactt cctgtatatt
       61 tcacattgct cgtaatccaa ttcaagctag gaattttact gaaaggcagt acccaagaag
      121 cattaattcg ttggttgtat gaatagggat tgcctaattg caaaatactt gtaagcacag
      181 aatctttcca agcctcgtat tcatctggat ataatttttt atttacacca ccgtcatttt
      241 cagtaatgcg agcctgattg cctgacgtaa atgctatctt gatagaacga gtcaaatccc
      301 atcgaatatc ggtatttgta ttccacataa attctttttg aaacgaagta ggtattaaat
      361 tgggagtact attatccgtt aaatttcgta atttttgttc gtaataatag cgtaccatac
      421 tcgtattaaa agaaattttg ttaggagcat aataaaaatt gatatcacgg atcaattgcc
      481 atgcaggttt cgcgaacagt ttactttcgg caaaaggctc tacaggtttt ggattccaac
      541 tatagtcata caccaatgaa gcacggtaat tttgctctat atctctttct gtcgtaggat
      601 tttgtctttc tgtttcagaa taagcataat taacagtaac attagcaggg tcgtaaggca
      661 taggcgtttt gctttttatc ccaatcgttg cattggacac agatatactt tgattggtaa
      721 caacatccaa tgatctatcg cgaatagagt cgcgttgcgt tttaagttca gcagcgtcta
      781 aagcttcttc taaaagtaca tctttatcaa gaggattgta ttgtggacta gatacttgct
      841 gagcgtacga ataagcgata ggcaaacgta cacgagcacg ttgaggaaaa aatctaccca
      901 tatcaaatac ggatgataca ttgtattcgt aataatcgtc aatacgacga tccattacat
      961 tttgctctat gccaccaaat ccaacagttt ctaccctacc agacacattt actctaccaa
     1021 aatcagacag attaacacct aaattagcta aagctgcaat acctccttct tcgttaaatt
     1081 ccgttaaacg caactcattt acccatactt ccgcagattt tacatcgcga gaggaatttc
     1141 gtaccccaat cataataacc tctacttctc ccaaatttgg atttcctaca atagtcattt
     1201 tattgcttgg cttagtaggg tcgtactgat aatatggcgt cgtattagaa acaacagatc
     1261 cagttcggtt tttttcttga tttcgtttaa tttttaaatc agtaagtaat tcaagaggaa
     1321 aatcaaacat attatcagag ggccatactg tttctctatc cgaagtttta ttggttgaat
     1381 aattacctgg aggagttaat gtaagaggaa tctcatattc gtagtaatta tttctaaaat
     1441 cagatcccaa acgaataaat acacgcattt cgttatctac taattgcgtt gtgttatcag
     1501 ccaaagcctc agcgtgaacg aacatctgca ttcgtttgta ctgacgcata tctacgcccg
     1561 atgttttgta tacggcacgt gcatcgccag gcgataattg caaaatacgc atggataagg
     1621 cttgttcgtt atcctgacga atttgctgct ggctagggtc aataacacga ctaactccag
     1681 ggggtaatac ataattgaca ggagttttat ctgcattttc ttcaatattg acagcagaca
     1741 tagatatgga ggctgtggaa gtaggtgttt tatctaagga atataattct tttgtgtatg
     1801 ttttccactg tccttttacc aattgtaacg tagcaaagcg aagcattaca tcttcttcaa
     1861 agtcggttaa aaacatacgc ataaaacgaa tagacttaaa atcgcgaata ccaccaatag
     1921 ccgtatagtt ctgtgcatca cgaattggaa tcttaaattg ataccatttt acttgctctg
     1981 ttttgccatt ttttagctct acggtagaag ttacaacatc tgcaatataa tttttcccta
     2041 ccaccatttc ttcgggacgt aacggcacct tatattgata atatttttcg tattcgttga
     2101 gcgtattatc attattaata tcttctacat caggcaaatt ggtagccgac gtggtaaagt
     2161 cttcgtttgt atcagcctgt gcaggagaat taccttcagt tccgttatag cgtttgtagc
     2221 gttctaatac tcctttttgt tggttatcgt aatacgagct tctataatgc gaaaaattat
     2281 cccctgctgg atcgttgagg ggagaaaatg gatcgttttc gaatttctgt aaggcgtctg
     2341 gcgttaatgt tgccttcaat tgattaataa aagtttggta agccccaaaa gtaaactctt
     2401 cattggtagt taatccattc aatccaacat cttgcaattt tcgtgcatta gcctcattat
     2461 caaatgcata cactaccgct tgacgtgatg gcacttttcc ccaattggtt gtttcagctt
     2521 gtggctgagc atcagttacg ggtaagccat tttcaaaaaa ctttctgcca tcttttaaaa
     2581 tatcttccga aatatctcct aaattgacat ataaatctcc acctttcatc acatgtaaag
     2641 tatcgtacac aaagggatcg agcaaccaaa actccatgta ttcgatattg ttgttttcaa
     2701 aatcggttgt ttctaaccgt ctcatcatac caccccattt cccttttggg ttagttaact
     2761 ttccttctga gtcataacta gttggataaa tattataggg tccacgctca gtaggataat
     2821 acgctacatt taatgtttga atataccccg attgtccata caacaattgt cgattaggga
     2881 aaatttcacg ttctctgatt tcacgcacaa aatgatttga tagctgttct ttgtcatttt
     2941 taatatgcaa tggcgttcga gaagtattgc gtgtaaaaat tgggtcgatg taataccagg
     3001 caagcaaggc tctatttttg ccatactcaa tatcatttac caaggctgcc tctggaaata
     3061 aaccacctgt tggatgataa ggagtactag ctagccccca catttgagga tgacggatat
     3121 caaacgctat tttggtagat tcaaaatcat ctaaataagc aattccttgt tcgtctattg
     3181 ttttggaatg tccaggaatc atttgagcaa actctgcatt taaattgata gaagatggct
     3241 ctgttaaatt gagcaaagga attttatcta acagattagt taaccattgg gattgggtag
     3301 tataagaagt atttagcccc cagattgtat ttgaaatagg ttcatttccc atgaggacct
     3361 tttgggtaag aggtttttct gacaaatgca taaaagtacc tcctacatta aatttatcgc
     3421 taaatgcata atccaaatgg gttcccacca aggtcttacg ttgcatacta aagagcgatt
     3481 tattttccaa attaatactt attggtgtgc ctgactcaat aatatcttga ttcaaaatat
     3541 tgacaacccc catcatataa tctactgtat aatcagtatt ttctgttagc tgacgcccac
     3601 cagcagttac ctttactgat ccttgaggca cattcattgc atttaaccga atttccgatc
     3661 ccgaagaaga gcggtattgc cccaccaagc gaaatttatt tttttctgcc acttctttgg
     3721 ctacaacaat agtagaatcg tacaactcct gaaacacaaa ttcgtttgct accacatcat
     3781 catcaaaagc tttacgcaaa tgagagccaa aaggctcaac aaccggaaat ataatacgac
     3841 cattttcggg aataacagta aaattttcaa caaaatcaaa cactccgtct ggattttgtt
     3901 gagagcgaga gtctaatcgg tctaaattca tcaccctcaa taaaattgta ttggctattt
     3961 ttcctttggg gatatagttt aaatccactc cagtagaatc actacggtac attacatcca
     4021 aacggaattt gtctttttgt acttgaaatg ctccaagcga atatacattc ttcatcatca
     4081 aatcccacat aggtacgttt ggcgattggg aaacaccttt tagaagtttc agtgttaaca
     4141 cattaggcga ctcaatacca tcagtagaaa attcccctac ttggtatact tttccattgt
     4201 atgtatattc gtatgctaca gccaatacct catctgtgcc taatgtgcgt cgcaacgaaa
     4261 taaaccctaa ttgtttgttt aatgaatatt ccgttggttc taatcgtcga gcactttcaa
     4321 tttttacgta atcctctcct cccacaatac caaaacctga caaactttgt cccagtgcat
     4381 tatttaccaa agaaatagta cgatatccat caatacctat cacttcacga tacaaactat
     4441 tagcatcatt atcaggaact aaagtggtag cagaaggtat ccaatgagta ttattgatgc
     4501 gtgttggttc agctaaatcc ataaaaccaa caatattacg agcctgatta aaatcaaccg
     4561 catttttgtt ggtaatccac acttcaattt tattaatcac tataccagag gtgatatgtg
     4621 gcaatttgct cataaaagaa tcataattat cgcgaaaata gtgtgcaagg aaaaaatgcc
     4681 tattttcatc atactgatca gcggcaatat caaaatctgt cgtctgtgct cctccgctta
     4741 aattaatagt ttttgattct gattcttgtt gcgataaaac ggtagttaca tttagcttac
     4801 caaattgcat ttccgtttta aacccaaata aagtagtatt accagtaatc aaacttccac
     4861 tcaatggcat acttacgtta cctgcttcaa ttttgcgtat aatatcgtct tctttgcctt
     4921 cgtaa
//
LOCUS       DAOSMY010000082         4917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17302_length_4917_cov_3.983340, whole genome shotgun sequence.
ACCESSION   DAOSMY010000082 DAOSMY010000000
VERSION     DAOSMY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17302_length_4917_cov_3.983340"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /locus_tag="PLD76_02890"
     CDS             <1..1875
                     /locus_tag="PLD76_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU26982.1"
                     /translation="SKNCENTTRRGGTSMIVPMMKYSFLVYHADYATFLSEIKKMGVL
                     HIQTKQNEQSAEIQDKYRSLTDLFKTIRSLEERKQADDDGNALLDESISGEEVLEKLR
                     AIEKEIEHKNHQLSSLEKEIQQLYPWGDFSALLIKEIASIGVTIRFLICKEKKYNPEW
                     EQVYCIEHISNHAGFSHFIVCQQHDEDISELLSLVDVDEITIPTITLSEAEAKQRVVQ
                     EEINSLQQQITAIATHHLMALKNLQNTWESAIADSNAYLQTEKTVDNYVMLIEGYIPE
                     PKTEELHAYLAMHDIVYTAEKPLDEEKVPVLLKNNRFTKVFEFIGSLYELPNHKELDL
                     TPFFAPFYMLFFGFALGDAGYGLLIVIAATVLKFKKPAMKGILTLAQFLGISTVIFGA
                     LTGTLFGINLMNVDIAWLQNAKQYMINTDKMFTLALALGTIQILFGMVLKVVNLSMAK
                     GFAYAYSTIGWLILIIGLGANYFLTQLQAYTPQSSTYIQYGVLSISGALILLFNNPKR
                     NILMNFLAGLWDVYGMLTGLIGDLLSYIRLFALGVSSAILGFVFNELAMSLRPDNIIL
                     GPIVMIIILVLGHGMTIFMSALGAFVHPIRLTFVEFYKNAGFVGGGKAYKPFAEKKEN
                     "
     gene            1908..2366
                     /locus_tag="PLD76_02895"
     CDS             1908..2366
                     /locus_tag="PLD76_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOU26983.1"
                     /translation="MEPIVLAYIGIGLMVGLAGTGSAFGCSIAGSASVGAMKKNADGF
                     GSYLLLTALPGSQGLYGFAGYFLMSNFFGLLTTDITWLQAGAVFGSGLAVGLVGLLSA
                     IRQGQVCANGIAAIGSGHDLAGKTLILAVFPELYAIISLAAAYMIGSGVI"
     gene            2463..3305
                     /locus_tag="PLD76_02900"
     CDS             2463..3305
                     /locus_tag="PLD76_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOU26984.1"
                     /translation="MQTHYTKYVTKASGEKQEFSAKKLVQSLMNAGANQETAKNILNE
                     IEKVMVDGMHTKKIYTIAFGLLKKERIQLALKYKLKKAIMELGPTGHPFENFIGQLFE
                     KRGYKTEVGVVVHGACIQHEMDVIATKPPFQYILECKYSQDQSKQVAIQVPLYVHSRV
                     EDIIDYRKKLPIYEGVEFITGVVTNTRFTTDSIQYGKCKGMYLLSWDYPANENLKQLV
                     DSLKLYPITILNSLSKKEKETLINAGYITCNQLLSNHEWDKELYLTPQKTLLIQKELD
                     LITS"
     gene            3404..3976
                     /locus_tag="PLD76_02905"
     CDS             3404..3976
                     /locus_tag="PLD76_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU26985.1"
                     /translation="METNEKKNVTEKVNEISTKSKLTFWGFIKKHPTFTTIIVALLSI
                     GAVILYKDASHAKWQKEMTEKASNQLSNTQEDLIKLVAKPLVWNIRAEMLRNNMEQVS
                     ILMTDMVRDNTIQYVHLIDTNGLVLLSTNKKLEGQIITNEFDSSLLKTTEPTTLVIEN
                     IVQVAAPVMGVDRQFGVLILGYQPTMLVLD"
     gene            4120..4659
                     /locus_tag="PLD76_02910"
     CDS             4120..4659
                     /locus_tag="PLD76_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU26986.1"
                     /translation="MKRIKTFSIICSTMAFFSLTACSTIPKGVEAVTAFEQERYLGKW
                     YEIARFDFRFERNLNNTTAFYELNSDNSIKVVNRGYNYVTNEWKEATGKAKFVEKNDV
                     AMLKVSFFGPFYAGYNVIAIDKNYQYALVSGSSLKYLWILSRTPQLPQNIKEEYLQLA
                     HEKGFKVENLIWVEHNQQE"
     gene            4664..>4917
                     /locus_tag="PLD76_02915"
     CDS             4664..>4917
                     /locus_tag="PLD76_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOU26987.1"
                     /translation="MMELYTNIAFKTSELVTKTYSTSFSIAISCLPYETKKAIYAIYG
                     FVRFADEIVDTFEKTQQKNLIRDFEADYQKALETGISMNP"
BASE COUNT         1704 a          772 c          909 g         1532 t
ORIGIN      
        1 tcaaaaaatt gtgaaaacac gacaagaaga ggaggcacaa gtatgatagt acctatgatg
       61 aaatattcgt ttttggtgta tcatgccgat tatgctacat ttctttctga aataaagaaa
      121 atgggcgtac tgcatataca aactaaacaa aacgaacaat cggctgaaat acaagataaa
      181 taccgttctc ttactgattt attcaaaact atacggagtt tagaagaacg aaaacaagcc
      241 gatgatgatg gtaacgcttt attggatgag tcaatttcgg gcgaagaagt gctggagaaa
      301 cttagagcta ttgagaaaga aattgaacac aaaaatcatc aacttagttc tttggagaaa
      361 gaaatacaac aactgtatcc atggggagat ttttctgcac tattgataaa agaaattgct
      421 tccattggtg ttacaattcg ctttttaatt tgtaaagaaa aaaaatacaa tcctgaatgg
      481 gaacaagtgt attgcatcga acacatatcc aatcatgcgg gcttttctca ctttatcgtt
      541 tgccaacaac acgacgaaga catcagcgaa ttactatcct tggtagatgt agatgaaatt
      601 actataccca ccataacctt gtcagaggca gaagctaaac agcgtgtggt tcaagaagaa
      661 atcaacagtc ttcagcaaca aatcacggct attgctacac accatcttat ggctttaaaa
      721 aatctacaaa atacatggga atcggctatt gctgactcaa atgcttattt gcaaacggaa
      781 aaaactgttg ataattatgt catgttgata gaagggtata ttcctgaacc taaaacagaa
      841 gagctacacg cataccttgc gatgcacgac atagtgtaca cagcagaaaa accgcttgac
      901 gaagaaaaag ttcctgtgct acttaaaaat aatcgtttta caaaagtttt tgagtttatt
      961 ggcagtttgt acgaattacc caatcataaa gaattagatt taacgccttt ctttgcccca
     1021 ttttatatgt tgttttttgg ttttgctttg ggcgatgcgg gttatggatt attgatagta
     1081 atagccgcta cagtactaaa gtttaagaaa cctgctatga aaggcatact tactcttgca
     1141 cagttcttag gcatttctac ggttattttc ggagcactta ctggaacatt gtttggtatc
     1201 aatctaatga acgtagatat tgcatggtta caaaatgcaa aacaatacat gataaatacg
     1261 gacaaaatgt tcactttagc attggcttta ggaactattc aaattctctt tggcatggtg
     1321 ctaaaagtgg taaatttgtc gatggcaaaa ggttttgctt atgcctactc tactattgga
     1381 tggttgatac taattattgg tttgggtgca aattattttt taacccaatt acaggcttac
     1441 acaccccaat cgtcaacata catacaatat ggagtattaa gcatttctgg agccttgatt
     1501 ttattattca ataatcctaa acggaatatt ttaatgaact ttttagctgg gttgtgggat
     1561 gtatatggca tgcttacagg cttgattggc gacttattat cctatatacg actatttgca
     1621 ttgggggtat ctagtgctat tttaggattt gtgtttaatg aattggctat gagtttacgt
     1681 ccagataata tcatattagg tcccatagta atgataatta tcttggtatt aggtcacgga
     1741 atgacaattt ttatgtctgc attgggtgct tttgttcatc ctattcgatt aacatttgta
     1801 gaattttata aaaatgcagg atttgtaggt ggaggaaaag cctacaaacc gtttgccgag
     1861 aaaaaagaaa attaataaga ataacaacaa ttaaaaaaag aaaaaaaatg gaacctattg
     1921 ttttagctta catcggaatt ggattaatgg ttggattagc tggcacagga agtgcgtttg
     1981 gctgttcaat agcaggtagt gcctctgtgg gggcaatgaa aaaaaatgcg gacggatttg
     2041 gttcgtattt attattaact gcattgcctg gtagtcaggg attatatggc tttgcaggat
     2101 atttcttgat gagtaatttc tttggattac ttacaacaga cattacatgg ttgcaagcag
     2161 gtgctgtatt tgggtcaggt ttggcagtag gtttagtggg tcttttatca gctatacgcc
     2221 aaggtcaagt atgtgccaat ggtattgctg ctattggttc aggacatgat ttggcaggaa
     2281 aaacattaat cttggctgta ttccccgaat tatatgccat catttcatta gctgctgcgt
     2341 atatgattgg gtctggcgtg atataaaaac attaatgcaa aggtattgct ctattatttt
     2401 ttcataaagg tagaactctg tttctgcctt tatttgttat tcaaccctca tgtaactatt
     2461 ctatgcaaac acattataca aagtatgtta ctaaagcatc tggcgaaaaa caagaatttt
     2521 cagccaaaaa attagtacag tcgcttatga atgcaggtgc caatcaagaa acagccaaaa
     2581 atattttgaa tgaaatagaa aaagtaatgg tagatggcat gcatactaaa aaaatatata
     2641 ctattgcatt tggactatta aaaaaagaac gtatccaact agcattaaag tataagttga
     2701 aaaaagccat catggagctt ggtcctactg gacatccatt tgaaaatttt attggtcagt
     2761 tatttgaaaa aagaggatat aaaactgagg ttggggttgt tgtacatgga gcctgcattc
     2821 aacacgaaat ggatgtaata gccacgaaac ctccctttca atatatctta gaatgtaaat
     2881 actcgcaaga ccaatcaaaa caagtagcca tacaagtacc cttatatgta cactcgcgtg
     2941 tagaagatat tattgattat agaaaaaaat tacccattta tgagggagta gaatttatta
     3001 caggtgtggt tacaaatacc cgctttacta cggatagtat ccagtatgga aagtgtaaag
     3061 ggatgtattt attatcgtgg gattatccag ccaacgagaa tttgaaacaa ttggttgatt
     3121 ctttaaagtt ataccctata accatactta attctctatc aaaaaaagag aaagaaacat
     3181 taataaatgc tggatacatt acatgcaatc aattattatc taatcatgaa tgggacaaag
     3241 agctctatct aacacctcaa aaaacactac tcattcaaaa agaattagat ttaataactt
     3301 cataaaaagg aacttgtttt tatagaaaaa aattttttat tcctctatta ttgcataatt
     3361 gtattttctt tgttatattt gtgtgtatat atacttatca attatggaaa caaacgagaa
     3421 aaaaaatgtt actgaaaaag taaatgaaat atccacaaaa tccaaactta ctttttgggg
     3481 atttataaaa aaacacccta cgtttactac tatcattgtt gcattactaa gcattggagc
     3541 tgtgattttg tataaagatg caagccatgc taagtggcaa aaagaaatga ctgagaaagc
     3601 tagtaatcaa ttatctaaca cacaagaaga tttaataaaa ctagtagcga aacctttagt
     3661 ttggaatatt cgggcagaaa tgctacgcaa taatatggag caagtttcaa tattaatgac
     3721 agatatggtg agagataata ctattcaata cgtccattta attgacacga acggattagt
     3781 tcttttatca accaataaaa aactagaggg acaaattatt accaacgaat ttgacagttc
     3841 tttgcttaaa accacagaac caacaacttt ggtgatagaa aatatagtac aagtagcagc
     3901 tcctgtaatg ggggttgata ggcaattcgg tgttttaata ctcggttatc aacctaccat
     3961 gctggtttta gactagcaaa aagatatact ctaaaaaagg tgagaattgc ttctcgcatt
     4021 ttttagtaca tagtacacca taaagctgtc gcatttcaaa gaatttcttt tgaaatagta
     4081 ttcatttttc ccttatcttt gtgtaaataa ttatcggata tgaaaagaat aaagaccttc
     4141 agcattattt gtagtacaat ggcttttttt agcttaacag cttgtagtac tatacccaaa
     4201 ggagtagaag cagtaacagc ttttgagcaa gaacgatacc taggaaagtg gtatgagata
     4261 gcacgttttg acttccgatt tgagcgtaat ctcaacaata caactgcttt ttacgaatta
     4321 aattccgaca atagcattaa ggtcgttaac cgcggttata attatgtaac caacgaatgg
     4381 aaagaagcaa cggggaaagc aaagtttgta gaaaaaaatg atgttgctat gctcaaggtt
     4441 tctttttttg gtccttttta tgcaggttat aatgtgattg ctatcgacaa aaattatcaa
     4501 tatgcattgg tatctggtag tagtcttaaa tacttgtgga tattatctcg cacacctcag
     4561 ctaccacaaa acatcaaaga agaataccta caacttgctc acgaaaaagg ctttaaagta
     4621 gaaaatttaa tttgggtaga acataatcaa caggaataat tgtatgatgg aactatacac
     4681 aaacattgca tttaaaacaa gtgaattggt tactaaaacc tatagtacct ctttttctat
     4741 tgctattagt tgcttgccat acgaaacaaa aaaagccatt tatgctattt atgggtttgt
     4801 acggtttgcc gacgaaattg tggatacttt tgaaaaaact caacaaaaaa acctgattcg
     4861 cgattttgaa gcagattacc aaaaagcttt ggaaacgggc attagcatga accctat
//
LOCUS       DAOSMY010000083         4907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17352_length_4907_cov_3.083265, whole genome shotgun sequence.
ACCESSION   DAOSMY010000083 DAOSMY010000000
VERSION     DAOSMY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17352_length_4907_cov_3.083265"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3078)
                     /locus_tag="PLD76_02920"
     CDS             complement(<1..3078)
                     /locus_tag="PLD76_02920"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU26988.1"
                     /translation="MNKKSYSFPLLFNHSIRHRLVAFSILLLLSALFATTHAQPLHIA
                     GSSTYYNELLGGSATNFGVPLTLENRRNTSVGVKTCSGKTTGTGWIKFVQETNSWNTQ
                     WNGGASAFTLSGNTYMATAGKYDVTSSGGGNINVSITKDLFYTFIIGQNTTSDNDVSV
                     LVTAQAPRNVTTITHSTPQEGQAVTVTSTLNNSISTTSGNGAQYVYLAYSVNGGTYTS
                     TPMTVTATNTVYTGTIPSNVHVADNTISYYTYITTVDGLTPSTTGFDFLCLSQSYVNT
                     YNLNGGTSTPITIKAKRPSSWSNDMYLFFWGGEITSPSWDESSKMNSLGEGWYSFTLP
                     SEITASNIIFRNGITSGLNNQSDDINAVSQSTCYNLSQSGSSKATASQVTCPVIVVGA
                     PTISAHKATVCENNFTDFASLYVSDYGILGSETGYAGGITFLWERSTDNVTWTTFTDG
                     RKATINNIRPTRTGYYRCKMTRFSTSTNSNEDIYSNVIQVTAGSNCTSRTVCSKLPVI
                     VVQTNVESFPTGDFLASNAAFQNAKKKISVDVKIIWDDTKDDTDNTMTEADFTNPNRL
                     YYDKKARMNYRGSSSLNYSKKNYAFVVGDDSCTLDGKWIKDTKKMFNINEVNKERDFI
                     LYAAYSDPTMMRNKLALDLYSDMTGSWNSKSRYVELYVDGEYKGVYIFMEKVKEDKGR
                     VQVQEDGYIFKFDKTDVWDRTTSTEGARSTFVSTITGTQGIPTYGITVDHGFEIDYPE
                     WKEEDFTPEQWTTKWTYLRNRINDFENALKNGNYTAVNQYIDYESWADYFIMQEFAKN
                     IDGYRVSVWFTMETATSPIKMTPLWDNELAFNMKNITGKGNTSTTGLMVENSETRTDD
                     FAIPFWWTGEGKTNVGKGLLDDKCFKAKVKERWLKHTTTGGALSQTNIRAKIDDMHTS
                     LLASSCSSTPVSREFAANARWSTSWAADTTNINTWINPTTGRRKGLDDIISTWPTILS
                     SATINTVNNVNLGDNVTFTATVTGTGTYTYAWYFCATNSMDKTTWTLLNANGTS"
     gene            complement(3188..4393)
                     /locus_tag="PLD76_02925"
     CDS             complement(3188..4393)
                     /locus_tag="PLD76_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOU26989.1"
                     /translation="MKKLLILFYTLIFTLNISAQMSIYFQQGEVDVKVPFVDIDSITF
                     NPVESNYLYTQLRIHNVNNTSTDLTLADIDNIYFKDSDILNPADITTQVLISYNGNSI
                     STTNPLAGNGVSISNTGGNVVVTSTVAGVEYILSGTTSNGSFKIYSDFEFKLTLNGAT
                     ITSTFGPAINIQSSKRVFVDLVDGKTSTLTDSSNYPDPLTATEDTKGCCFSEGQLIFM
                     GNGNLLVNAKAKHAIASDDYIRVISGNILVLEAMRDGLKSNDWIIIDNGYVSINATDD
                     GIQSDRGHIIINGGTVEVTSFDEGIYASYDPLLEGDYTIKPYIIINGGDITITTTAES
                     AHGIRTGDDITINGGNMSITTYGARSDGMKTEGKITLNGGTAIIKAFAKCMDYGTLID
                     NGNVVTCLY"
     gene            complement(4390..4860)
                     /locus_tag="PLD76_02930"
     CDS             complement(4390..4860)
                     /locus_tag="PLD76_02930"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU26990.1"
                     /translation="MKKIISSILLAYIFVSSGFAMTHMRVEKNDASEEVIQLNTIRTI
                     RFQGENIQFNYKNGNSDLFAANTLKSLQFSSIPSSIEEKTSAELFFYPNPATDYIYLK
                     NLATEEAMISIYQIDGVIVMKGKQSLHTGIYVGNLASGLYLLEVNKQTYKFRKL"
BASE COUNT         1458 a          905 c          829 g         1715 t
ORIGIN      
        1 tgatgtacca tttgcattta atagcgtcca tgtagtttta tccattgagt tggttgcaca
       61 aaaataccaa gcatatgtgt aagtgcctgt tcccgttact gttgcagtaa atgtaacatt
      121 atcaccaaga tttacattat tgacagtatt aattgtagca gatgataaaa ttgtaggcca
      181 agtagatata atatcatcta atccttttct tcttcctgtt gttggattta tccacgtatt
      241 tatatttgtt gtgtcagcag cccaagatgt gctccagcga gcattcgctg caaattcgcg
      301 actaacaggt gtagatgaac aactcgatgc taataacgag gtatgcatat catcaatttt
      361 agcacggata tttgtttgag acaacgcacc tcctgtggtg gtgtgtttta accaacgttc
      421 ttttactttt gctttaaagc acttgtcgtc aagcagtccc tttccaacgt ttgtttttcc
      481 ttcacctgtc caccaaaaag gtatagcaaa atcgtctgtc cgagtttcag agttttcaac
      541 catcaaacct gtagtggatg tatttccttt acctgtaata tttttcatat taaaagctaa
      601 ttcattgtcc cacagcggag tcattttgat aggagatgtg gctgtttcca tcgtaaacca
      661 tacagaaacg cgataaccat caatgttttt tgcaaattct tgcataataa agtaatcagc
      721 ccacgattca taatcaatgt attggttgac ggcagtataa ttgccatttt ttaaggcgtt
      781 ttcaaaatca tttatgcggt tacgcagata agtccatttt gtggtccatt gttcaggtgt
      841 aaaatcttct tctttccatt ctgggtagtc gatttcaaag ccatgatcaa ctgttattcc
      901 gtaagtcggt ataccttgcg ttcctgtaat agtcgacaca aaagtacttc tagctccttc
      961 tgttgaagtt gttctatccc aaacgtctgt cttatcaaac ttaaatatat atccatcttc
     1021 ttgtacttgt acacgtcctt tgtcttcttt tactttttcc atgaatatat atacgccctt
     1081 gtattctcca tctacataca gttctacata gcgagattta ctattccaag aacccgtcat
     1141 gtcgctatat aaatccaagg ctagtttgtt tcgcatcata gttgggtcag aataggctgc
     1201 atacaatata aaatcgcgtt ccttgttgac ttcattgatg ttaaacattt tttttgtgtc
     1261 ttttatccat tttccatcta atgtacaact atcatctcct acaacaaatg cgtagttctt
     1321 ttttgaatag tttaaactag aagaacctcg gtaattcatt ctagcttttt tatcataata
     1381 caaacgattt ggattggtga aatcggcttc tgtcatagta ttatccgtat catcttttgt
     1441 atcgtcccaa attattttaa catcaacgct tattttcttt ttagcattct gaaaagcggc
     1501 atttgatgct aaaaagtcgc cagttggaaa actctctaca tttgtttgaa caacgattac
     1561 aggcaattta ctacaaacag tacgtgatgt acagtttgac cctgctgtaa cttgtattac
     1621 attgctataa atatcctcat tgctatttgt ggaggtagaa aaacgtgtca ttttacaacg
     1681 gtaatagcct gttctagttg gtctaatgtt gtttatcgta gcttttcttc catcggtaaa
     1741 cgtcgtccaa gtaacgttgt ctgttgaacg ttcccataaa aacgtaatcc cgccagcata
     1801 tcctgtttct gaacctagta tgccataatc acttacatat aaagatgcaa aatccgtaaa
     1861 attattttca catactgtag ctttatgtgc tgaaatggta ggtgctccca caacaataac
     1921 aggacatgtt acttgcgaag cagttgcttt tgaagatcca ctttgactca gattataaca
     1981 tgttgattgt gatactgcat tgatatcatc tgattggtta tttaaccctg aggttatccc
     2041 atttctaaat ataatattac tagctgtaat ctcagagggc aaagtaaatg aataccaccc
     2101 ctcaccgagg ctattcattt tgctagactc atcccaactt ggagaagtta tttcacctcc
     2161 ccagaaaaat aaatacatat cattgctcca agagcttgga cgtttggctt tgatagttat
     2221 tggggtagat gttcctccat taaggttgta tgtatttaca taagattggc taaggcaaag
     2281 aaaatcaaag cctgtagtag atggggttaa tccatccaca gtggtaatat aggtgtaata
     2341 cgaaatggtg ttatcagcta cgtgaacatt tgaggggatt gttcctgtat ataccgtgtt
     2401 tgttgcagtt actgtcatcg gtgtagatgt gtatgttcca ccattaacac tgtatgctag
     2461 atatacatat tgtgctccat tacccgaagt agtggatatg gaattattga gtgtgctggt
     2521 aactgttact gcttgtcctt cttgtggagt agagtgtgtt attgtcgtaa cattccgcgg
     2581 ggcttgtgca gttactaata cagacacatc gttatcagac gttgtatttt gaccaattat
     2641 aaaggtgtaa aacaagtctt ttgttatact tacattaata tttcctcccc cacttgacgt
     2701 tacatcatat ttgccagcag tagccatata tgtgttgcca gaaagtgtaa acgctgatgc
     2761 acctccattc cattgagtat tccaactgtt ggtttcttgt acaaacttaa tccaacctgt
     2821 accagttgtc tttccagagc aggttttgac accaacactg gtatttcggc gattttctag
     2881 ggttaacgga acaccaaagt ttgtagcaga accacccaat aattcattat aatacgttga
     2941 actcccagct atgtgtagag gttgtgcatg ggtagtagca aacaaggcac ttagcagtaa
     3001 taaaatggaa aaagctacta agcgatgacg aatagaatga ttaaatagca atggaaaact
     3061 atatgatttt ttgttcataa tacgtagtta aggttggaga aataaatgta aatatgttga
     3121 aagaaatcta cctactatca cacgaatgtg agtttcctat gatagtaggc aagtagatgt
     3181 gaaaatatta gtagagacaa gttactacat ttccgttatc aataagcgta ccataatcca
     3241 tacatttagc aaatgcttta ataatagctg ttccaccatt taaagtaatt ttaccctctg
     3301 ttttcatgcc gtcagaacgg gctccatagg tagtaattga catgtttcct ccgttgattg
     3361 taatatcatc tccagttcga ataccatgag ccgattccgc tgttgtggtt atcgtaatat
     3421 ctcctccatt aatgataatg tatggtttta ttgtgtaatc accttctaag agtgggtcgt
     3481 atgatgcata tataccctcg tcaaaactag ttacctctac ggttccacca ttaataataa
     3541 tatgtcctct atctgattgt atgccatcgt ctgttgcatt tatagataca tatccattgt
     3601 ctatgattat ccagtcattt gattttaacc catccctcat tgcttcaaga actaaaatat
     3661 tgccgctaat aacacgtata taatcatcgc ttgcaatagc atgctttgct tttgcattaa
     3721 ccaataaatt accattaccc ataaaaatta attgtccctc actaaagcag cacccttttg
     3781 tatcttctgt tgcggttaat ggatctggat agtttgaact gtcagtaaga gtacttgttt
     3841 ttccatctac taaatcgaca aatacgcgtt tacttgattg aatattgatg gcaggtccaa
     3901 atgtactggt tatagtagct ccatttaatg ttaatttaaa ctcaaaatca ctataaattt
     3961 taaatgatcc attgctcgtt gtcccagata aaatatattc gacacctgct actgtagatg
     4021 taactactac atttccccct gtgttactaa tagatacccc gtttcctgca agagggttgg
     4081 tggttgaaat actattccca ttatacgaaa taaggacttg ggttgttata tctgctggat
     4141 ttaaaatatc actatcttta aaataaatat tatcaatatc tgctaatgtt aaatctgtgc
     4201 ttgtgttgtt aacgttatgt atacgtaatt gagtgtaaag ataattagat tcaacaggat
     4261 tgaacgtaat gctatcaata tcaacaaatg gtacttttac atccacttct ccttgctgaa
     4321 aataaatact catttgagcc gatatgttta aagtaaaaat gagtgtataa aatagtataa
     4381 gtagcttttt cataattttc taaatttata agtttgttta tttacttcta aaaggtaaag
     4441 tccacttgct aaattgccaa cataaatacc tgtatgaaga gattgtttcc ctttcatcac
     4501 gataactcca tcaatttgat agatagaaat catagcttct tctgttgcca agttttttaa
     4561 ataaatataa tcggtagctg gattgggata aaaaaataat tcagcggaag ttttttcttc
     4621 tatactagat ggaatagaag aaaattgtaa gctttttaat gtgttcgctg caaataaatc
     4681 agaatttcca tttttatagt taaattgaat attttctcct tgaaaacgaa ttgtgcgaat
     4741 ggtgtttaac tgaataactt cttcagatgc atcatttttc tcaacacgca tatgtgtcat
     4801 tgcaaatcca ctagatacaa aaatgtatgc aagcagaata ctagaaataa tttttttcat
     4861 agatgtataa ttttatggtt aattcttata aacaagtata ttcccct
//
LOCUS       DAOSMY010000084         4904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17361_length_4904_cov_3.554341, whole genome shotgun sequence.
ACCESSION   DAOSMY010000084 DAOSMY010000000
VERSION     DAOSMY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17361_length_4904_cov_3.554341"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLD76_02935"
     CDS             <1..418
                     /locus_tag="PLD76_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU26991.1"
                     /translation="TFPEHPLRGKNHPELGVRFPDMSQAYSKELISKAKKIAARNTIK
                     VVEGVYLGTSGPTFETPAEYVYFRNIGADTVGMSTVPEVIVAVHSGIKVFAMSIITDL
                     GVPGKIVEVSHEEVQHIANQVQPKMTQIMKELIAEL"
     gene            572..1852
                     /gene="eno"
                     /locus_tag="PLD76_02940"
     CDS             572..1852
                     /gene="eno"
                     /locus_tag="PLD76_02940"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU26992.1"
                     /translation="MCRIEKIVAREVLDSRGNPTVEVDVTLESGIVGRAAVPSGASTG
                     ENEAIELRDGDKGRYLGKGVLKAVANVNNVIAPELIGMCAMEQRAIDKKMIALDGTKT
                     KSKLGANAILGVSLAVAKAAAEYLGLPLYRYIGGTNAFTLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAKSFREGLRMGAEVFHSLKKVLHDKGLSTAVGDEGGFAPNLAGGTEE
                     ALESILTAVKNAGYVPGKDVTIGLDCAASEFYKNGIYDYSKFEGPNGKKRTSAEQADY
                     LAELIANYPIDSIEDGMGEGDWDGWKLLTDKIGSKCQLVGDDLFVTNVEYLKKGIDLG
                     CANSILIKVNQIGSLSETLDAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQI
                     KTGSLSRSDRMAKYNQLLRIEEELGDLAVYGYKK"
     gene            complement(1929..3161)
                     /locus_tag="PLD76_02945"
     CDS             complement(1929..3161)
                     /locus_tag="PLD76_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU26993.1"
                     /translation="MEILGNLYEMTAFSNIIAQPQFLLMYAIAFLLLYLGIKKEFEPL
                     LLIPIAFGVLIANFPGGQMGIIQANSEGLVEVGGEMKSIWQMPLHDIAHEMGIMNFLY
                     YMLIKSGFLPPIIFMGVGALTDFGPMLRNIRLSIFGAAAQLGIFTVLIGAILLGFTPK
                     EAAALGIIGGADGPTAIFTTIKLAPHLLGPIAIAAYSYMALVPVIIPLVVKLLCTEKE
                     LRINMREEEKKYPSKTQIKNMRVTKIIFPIAVTTIVALFVPSSVPLVGLLMFGNLLKE
                     VGTNTLRLSDAASNAILNTATIFLGLCVGATMTTEAFLNWQTIGIIIGGFLAFALSIS
                     GGILFVKLINLFSVKKINPLIGATGLSAVPMASRVANEIALRYDKKNHILQYCMSSNI
                     AGVIGSAVAAGVLISFLS"
     gene            complement(3174..>4904)
                     /locus_tag="PLD76_02950"
     CDS             complement(3174..>4904)
                     /locus_tag="PLD76_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOU26994.1"
                     /translation="IIRMGCFARVETNGGGFEQINLLFGENPNVAVRQWCKPFNDAGI
                     QTHMLDRSLNALRMYPVSEDVRRLFYKVKKAQGTDIARSFCGLNDTRNIIASIRYAKE
                     AGMIAQAALSLTVSPVHTVDYYCAMADELIANGADEICLKDMAGIGRPVSLGKIVAYI
                     KKKYPTIIVQYHGQTGPGFTPASILEVCKAGCDIIDVGMEPLSWGTGHADVIMVREML
                     KDAGFSVPEINMSAYMKVRALTQEFMDDFLGYFIPETNRHLQSLLIAPGLPGGMMGSL
                     MNDLKDNLVSINKAYQKQGKTTINTDELLIQLFDEVAYVWPRVGYPPLVTPYSQYVKN
                     LAMFNIIQMAKGKARWTMISDDTWDMILGKAGRLPGELAPEIKELATQQGRTFYEGDP
                     QILYPNVLENFRKEMIDNGWDMGKDEEELLELAMHPEQYRTYKSGKAKHDFEIELAQA
                     KKESQQRGDGSFVLPHVLEMTVNGEKYKVELAEFANSGLDSNVLLKENAEGEKVLAPL
                     EGRFFLTKSASDIPLKIGSQVKKGDIVCYVEAMKTYNAIQAETDGVIVDFVVANGDNV
                     SEDDVLLIIQ"
BASE COUNT         1620 a         1019 c          814 g         1451 t
ORIGIN      
        1 tacattccca gaacatccac tgagaggtaa aaaccatcct gaattaggcg ttcgttttcc
       61 agatatgagt caagcttatt caaaggaatt aatttcaaaa gcaaaaaaaa ttgcagcaag
      121 aaacacgatt aaggtagtag aaggtgttta tctcggcacc tctggtccta cttttgaaac
      181 tcctgccgaa tacgtttatt ttagaaatat tggggcagac accgttggta tgtcaacagt
      241 tcctgaagta attgttgccg ttcatagtgg cattaaggta tttgctatgt caattattac
      301 agatttaggg gttcctggta aaattgtgga agtaagccac gaagaagtac aacacatcgc
      361 taatcaggtg caacccaaaa tgacccaaat aatgaaagaa cttatcgccg aattatagct
      421 ttgtaacatt ggtgtaatga atatgctaag gggaaatatt aaaaacaaaa aataatacag
      481 acatatttaa tagtcaagat tttttttgta cttttgtacg tcaaattttg aaacacaatt
      541 agtgaatatc aatttataaa taaaaaagaa catgtgtaga attgaaaaaa ttgtagcccg
      601 cgaagtactt gattcgcgtg gaaatccaac tgtagaagtt gatgtaacgt tagagtcagg
      661 aattgtaggc agagctgctg taccatcagg tgcatcaact ggcgaaaacg aagctattga
      721 attacgcgat ggcgacaaag gtcgctactt aggcaaaggt gtattaaaag ctgttgctaa
      781 cgtaaacaac gtaattgcac ctgaacttat cggtatgtgt gctatggaac aacgtgctat
      841 cgacaaaaaa atgattgcat tagatggtac aaaaacaaaa tcaaaattag gagctaatgc
      901 tatcttaggc gtttctttgg ctgtagctaa agcagctgct gaataccttg gattaccatt
      961 ataccgttac attggcggta caaacgcatt tacgttgcct gtacctatga tgaatatcat
     1021 taacgggggc tcgcactctg atgctcctat cgcattccaa gaatttatga ttcgccctgt
     1081 aggtgctaaa tctttccgcg aaggcttacg tatgggtgct gaagttttcc attctttgaa
     1141 aaaagtatta cacgataaag gattaagtac tgctgttggc gacgaaggag gttttgctcc
     1201 aaatcttgct ggaggtactg aagaagctct agaatcaatc cttactgcag taaaaaatgc
     1261 gggatatgtt ccaggaaaag atgttacaat tggattggat tgtgctgctt ctgaatttta
     1321 caaaaacggc atctatgact attcaaaatt tgaaggtcct aatggaaaaa aacgtacctc
     1381 tgctgaacaa gcagactatt tagcagaatt aatagctaac tatcctattg actcaatcga
     1441 agatggtatg ggtgaaggcg actgggacgg atggaaacta ttaacagaca aaattggtag
     1501 taaatgtcaa ttagtaggtg atgacttatt tgtaactaac gtagaatact tgaaaaaagg
     1561 tatcgattta ggttgtgcta actctatcct tattaaagtt aaccaaatcg gatctctttc
     1621 tgaaacatta gacgctatcg aaatggctca ccgtgctggc tatacttctg ttacttctca
     1681 ccgctctggc gaaactgaag atgcaactat tgcagatatt gcggtagcta caaattctgg
     1741 tcaaatcaaa acaggttcgt tgagccgatc tgaccgtatg gctaaataca accaactact
     1801 tcgtatcgaa gaagaattag gtgacttagc tgtatatggc tacaaaaaat aaacctactt
     1861 atagtatact aaataaagcc tagcgaaatt cgctaggctt tattattttt atacacatta
     1921 agtgtaaatt acgacaaaaa tgaaataaga actccagcag ctactgcaga acctattaca
     1981 ccggcaatat tactcgacat acaatattgt aaaatgtggt ttttcttatc ataacgtaac
     2041 gcaatctcgt tggcaacacg cgaagccatt ggcacagcac ttaagccagt agctccaata
     2101 agggggttaa tctttttgac agaaaaaaga tttataagct taacaaacaa aataccacct
     2161 gaaatagaca acgcaaacgc caagaaaccg ccaataataa tccctattgt ttgccaatta
     2221 agaaaagctt ctgttgtcat tgtagcacct acacacaagc ccaaaaaaat agttgctgta
     2281 tttaaaatag catttgaagc tgcgtctgac agtcgcaaag tatttgtacc aacttctttt
     2341 agtaagttac caaacattag caatccaact aacggaaccg aagaaggcac aaacaatgct
     2401 acaatagtag ttacagctat cgggaaaata atctttgtta ccctcatatt tttaatctgc
     2461 gtttttgaag gatatttttt ttcttcttca cgcatattaa tacgcaattc tttttcagta
     2521 cacagcaact taacaacaag aggtataata acagggacta acgccatata cgaataggca
     2581 gcaatggcaa tgggacccaa taaatgtgga gccaacttta tagttgtaaa aatcgctgta
     2641 ggtccatctg ctccaccaat tatccccaat gcagcagcct ctttgggcgt aaaaccaagc
     2701 aaaatagccc ctatcagtac cgtaaaaata cccaattgtg cagcagctcc aaaaatggat
     2761 aatcggatat ttcgcaacat cggaccaaaa tccgttaaag ctcctactcc cataaaaatt
     2821 attggaggaa ggaaccctga tttaataagc atatagtata aaaaattcat aattcccatc
     2881 tcgtgtgcaa tatcatgcaa cggcatttgc caaatacttt tcatttcacc acccacttct
     2941 accaatcctt ctgagttagc ctgtataatc cccatttgcc cacctgggaa attggctatt
     3001 aataccccaa aggctatcgg aatcaacaat aatggctcaa attccttttt tatacctaaa
     3061 tacaacaata aaaatgcaat ggcatacatg agtaaaaatt gcggttgagc aataatatta
     3121 ctaaacgctg tcatttcata caaatttcct aaaatttcca tatatcatta agattattga
     3181 ataataagca acacatcgtc ctctgacaca ttatctccat tagcaactac aaagtcaaca
     3241 attacaccat cagtttcagc ttgaatagca ttatatgttt tcattgcctc tacataacaa
     3301 acaatatccc ctttcttaac ttgtgatcca attttcaagg gaatatctga ggctgatttt
     3361 gtcaaaaaga acctcccttc taaaggagcc aacacctttt ctccttctgc attttctttt
     3421 agaagaacat tcgaatctaa tccactattc gcaaattctg caagttctac tttatatttt
     3481 tctccattta cggtcatttc aagaacatgt ggcaatacga aagaaccatc tcctcgttgt
     3541 tgcgattctt tttttgcttg tgctaactct atttcaaaat catgtttagc tttccccgat
     3601 ttatacgttc tatattgttc tggatgcatg gcaagttcta acaattcttc ctcatctttt
     3661 cccatatccc atccattatc aatcatttcc ttgcgaaaat tttctaacac attcggatac
     3721 aaaatttgag ggtctccttc ataaaatgta cgaccttgct gtgtagctaa ctctttaatc
     3781 tcaggagcca attctcctgg caatcgtcca gctttaccaa gtatcatatc ccaagtatca
     3841 tcagatatca ttgtccaacg agctttccct ttagccattt gaatgatatt aaacatcgct
     3901 aaatttttaa catactgaga atatggcgtt accaaaggag gatagccaac gcgaggccac
     3961 acataagcca cttcatcaaa tagttgaatc aacaactcat ctgtattaat ggtagtttta
     4021 ccttgctttt gatatgcttt gtttattgat actaaattat ctttcaaatc attcatcaaa
     4081 ctacccatca taccgcctgg caatccaggt gctatcaaca gcgattgcaa atgtctattc
     4141 gtttcgggaa taaaatatcc taaaaaatca tccataaact cttgagtcaa tgcacgcact
     4201 ttcatataag ctgacatatt gatttctggc acactaaaac cagcatcttt caacatttct
     4261 cgcaccataa ttacatctgc atgaccagta ccccacgata aaggctccat cccaacgtca
     4321 ataatatcac aaccagcttt acaaacctcc aaaatagaag ccggagtaaa accaggacca
     4381 gtctgtccat gatattgcac aataatagtt gggtattttt tctttatata tgctactatt
     4441 tttcctaatg atacagggcg accaatgccc gccatatctt ttaaacaaat ttcatcggca
     4501 ccattagcta taagttcatc tgccattgca caataataat caaccgtatg cacaggagac
     4561 acagtaagcg acaatgcagc ttgtgctatc atacctgctt ctttggcata tcgaatagat
     4621 gctataatat ttcgggtatc gtttaaccca caaaatgaac gtgctatatc tgtaccttgt
     4681 gcttttttaa ccttatagaa taatcttctc acatcttccg acactggata catacgcaat
     4741 gcatttaaag acctatcaag catgtgagtt tgaatacctg catcattaaa tggcttgcac
     4801 cactgcctaa cagccacatt tggattttcc ccaaacaaca aattaatttg ctcgaatcca
     4861 cctccatttg tttcgacacg tgcaaaacaa cccatacgaa ttat
//
LOCUS       DAOSMY010000085         4885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17431_length_4885_cov_3.778468, whole genome shotgun sequence.
ACCESSION   DAOSMY010000085 DAOSMY010000000
VERSION     DAOSMY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17431_length_4885_cov_3.778468"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="rpoB"
                     /locus_tag="PLD76_02955"
     CDS             <1..634
                     /gene="rpoB"
                     /locus_tag="PLD76_02955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU26995.1"
                     /translation="SRIVRQEDMPFLEDGSPVDIVLNPLGVPSRMNLGQIFETVLGWA
                     GKELNVKFSTPIFDGATLEDLDEWTTKANIPRYGRTYLYDGGTGEKFDQPATVGVIYM
                     LKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAAHILQEI
                     LTVKSDDVMGRAKTYEAIVKGDPMPAPGIPESLNVLLHELRGLGLSINLE"
     gene            699..>4885
                     /gene="rpoC"
                     /locus_tag="PLD76_02960"
     CDS             699..>4885
                     /gene="rpoC"
                     /locus_tag="PLD76_02960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU26996.1"
                     /translation="MAFRKENKAKSSFSKISIGLASPEEILEHSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTDKKVRRERMGHIQ
                     LVVPVAHIWYFRSLPNKIGYLLGISTKKLDSIIYYERYVVIQAGTQSDLEVGDLLTED
                     EYLNIMDALPRENYLLEDTDPNKFIAKMGAEAIYDMLSRLDLDKISYELRDKASNDTS
                     QQRKTEALKRLQVVEAFRASKQRNKPEWMVVKVVPVIPPELRPLVPLDGGRFATSDLN
                     DLYRRVIIRNNRLKKLIEIKAPDVILRNEKRMLQEAVDSLFDNTRKSTAVKADANRPL
                     KSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKPFVI
                     RKLIERGIVKTVKSAKKIVDRKDPVVWDILEHVMKGHPVLLNRAPTLHRLGIQAFQPK
                     MIEGKAIQLHPLSCTAFNADFDGDQMAVHLPLGNEAVLEAQILMLASHNILNPANGAP
                     ITVPSQDMVLGLYYMTKGRSGALGEGLKFYSPKEVIIAYNERKVSLHANISVKTQDVE
                     NGVIVTKMIETTVGRVILNEYVPKKVGYINEILSKKSLRDIIGKIINVCGIADTANFL
                     DDIKSLGYQMAFKGCLSFNLDNIIIPKEKEELVQQGYAEVEEILANYNMGFITYNERY
                     NQIIDTWTHINSKLSNILMKQLSTDNQGFNSVYMMLDSGARGSKEQIRQLSGMRGLMA
                     KPQKSGAEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRL
                     VDVSQDVIIQEEDCGTLRGLVAVELKNNEEVVASLYERILGRVSVHDIQHPLTGKHIV
                     SAGEEITENTAKIIQDSPIERVEIRSVLTCESKKGVCAKCYGRNLSTNRMVQKGEAVG
                     VIAAQSIGEPGTQLTLRTFHVGGIASNVATESKIISRYNGILEIEELRTVSITEEGKT
                     HQIVVSRLAEFKIIEPTTKVVLTTQNIPYGAKLYFQSGVTVEKGAVICEWDPFNAVVI
                     SEVAGKMTFESMVENITFKTESDEQTGLKERIIIESKDKSKVPAAHILDEKGEILRTY
                     ALPVGAHIAITEGSKIKIGQILLKMPRAVGKSGDITGGLPRVTELFEARNPSNPAIVS
                     EIDGEITFGKIKRGQREIVVTSKSGEVKKYLVPLSKQILVQENDFVRAGNPLSDGAIT
                     PSDILAIKGPTAVQEYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVEIEDPGDTRF
                     LEKQTVDKQEFMEENDRIWGKKVVVNPGDSEVLMAGQIIAPRKLRDENSMLKRKDLRT
                     VEVRDAMSATSSAVLQGITRASLQTTSFMSAASFQETTKVLNDAAINGKVDKLEGLKE
                     NVICGHLIPAGTGQPEL"
BASE COUNT         1683 a          778 c         1025 g         1399 t
ORIGIN      
        1 gtctcgtatt gtacgtcaag aagatatgcc atttcttgaa gatggaagcc ctgtggatat
       61 agtactgaac cctttgggtg tgccttcgcg tatgaactta ggtcaaattt ttgaaaccgt
      121 attgggatgg gctggaaaag aattaaatgt aaaattttct acgccaattt ttgatggtgc
      181 tacattggaa gatttagacg aatggactac aaaagcaaac attcctcgtt atggtaggac
      241 atatttatac gatggtggaa caggtgaaaa atttgaccaa ccagcgactg taggtgttat
      301 ctacatgctt aaattaggac atatggtcga agataaaatg catgctcgtt caattggacc
      361 atactcactt attacacaac aaccacttgg aggaaaagct caatttggag gtcaacgttt
      421 tggtgaaatg gaggtttggg ctctcgaggc ttttggtgct gctcatattt tgcaagaaat
      481 attgactgta aaatcagatg atgtaatggg acgtgcaaaa acctacgaag ctattgtaaa
      541 aggagatcct atgcccgcac ctggcatacc agaatctctc aacgtattac tacacgaact
      601 tcgtggtttg ggacttagca ttaatttaga ataaaataag tggtttcaag atatgggaac
      661 attcccatat cttgacatct tttttaaata aaaaatatat ggcatttaga aaagaaaata
      721 aagcaaaaag cagttttagc aaaatatcaa tagggttagc gtctcccgaa gaaattttag
      781 aacactctag tggagaggtt ctaaagcctg aaactattaa ctatcgtact tataaaccgg
      841 aacgtgatgg attgttctgt gaacgcattt ttggtcctgt aaaagattat gaatgtcatt
      901 gtggaaaata caaacgtatt cgttacaaag ggattgtatg tgatagatgt ggagtggaag
      961 ttactgataa aaaagtacgt agagaacgta tgggtcatat tcaattagta gtacccgtag
     1021 cacatatttg gtatttccgt tccttaccca ataagatagg ttacttactt ggtatatcta
     1081 ctaaaaaatt agattcaatt atttattatg aaagatatgt tgttatccaa gcaggaacac
     1141 aatctgattt ggaagtagga gatttactta ctgaagatga atatttaaat atcatggatg
     1201 ccttaccaag agaaaattat cttttggaag atacagaccc taataagttt atagcaaaaa
     1261 tgggtgctga agctatttat gatatgttat ctcgtttgga tttagacaaa atttcttacg
     1321 agttacgcga caaagctagc aacgacactt ctcaacaacg caaaacagaa gcattgaaac
     1381 gcttacaagt agtagaagct tttcgtgcgt caaagcaacg caacaaacca gaatggatgg
     1441 tcgtaaaagt agtgccagtt attccaccag aattgcgtcc gcttgtgcct cttgatggag
     1501 gacgttttgc aacttcggac ttaaatgatt tgtatcgtcg tgttattatt cgtaacaatc
     1561 gtttgaaaaa gttaattgaa atcaaagctc cagatgtaat tctacgtaat gaaaaacgta
     1621 tgttgcaaga ggctgttgac tcattatttg acaataccag aaaatctact gctgtaaaag
     1681 cagatgcaaa cagaccatta aaatctttgt cagatagctt aaagggaaaa caaggacgtt
     1741 tccgtcaaaa cttattaggt aagcgtgttg actattctgc acgttcggta attgttgttg
     1801 gtcctgaatt aaaaatgcac gaatgtggtt tgcccaaaga tatggcagca gaactttaca
     1861 aaccatttgt aattagaaaa ctaattgaac gtggcattgt taagacggta aaatctgcaa
     1921 aaaagattgt ggatagaaaa gaccctgtag tgtgggatat attagagcac gtgatgaaag
     1981 gacatcctgt gctactaaac cgtgctccaa ctcttcaccg acttggtatt caagcttttc
     2041 agcctaaaat gattgaagga aaagctattc aacttcaccc tctttcatgt acagcattta
     2101 atgctgactt tgacggagac caaatggccg tacatttacc attagggaat gaagctgtgc
     2161 tagaggctca aattttaatg cttgcttctc ataatatttt gaaccctgca aatggagctc
     2221 ccattacagt accttctcaa gacatggtac ttggactgta ttacatgaca aaaggcagaa
     2281 gtggtgcatt aggtgaaggg cttaagtttt attctcctaa agaagttatt attgcctaca
     2341 acgaacgtaa agtatcgtta catgcaaaca tatctgtaaa aacacaggat gtggaaaatg
     2401 gagtaatcgt taccaaaatg atagaaacta ctgtaggacg tgtgatatta aatgaatatg
     2461 ttcctaaaaa agtgggttat atcaacgaaa ttttatcaaa aaaatcactt cgtgatatta
     2521 ttggcaaaat tatcaatgtg tgtggtattg cagatacagc gaactttttg gatgatatta
     2581 aaagcctagg ttaccaaatg gcattcaaag gctgtctgtc atttaactta gataacatca
     2641 ttattccaaa agaaaaagaa gaattagttc aacaaggata tgctgaagta gaagaaattt
     2701 tggctaacta taatatgggt ttcattactt acaatgaacg ctataaccaa atcattgata
     2761 cgtggacaca tatcaactct aagttgtcaa atattttaat gaaacagctt tcgacagaca
     2821 atcaagggtt taactcagta tacatgatgt tagattctgg tgctcgtgga tcgaaagaac
     2881 agattaggca gttatctggt atgcgtggtc tgatggcaaa accacaaaaa tcaggagctg
     2941 aagggggtca aattattgaa aacccaattt tatcaaactt taaagaaggc ttatcagtac
     3001 tggaatactt tatctctacg cacggtgcac gtaagggtct tgcagataca gcgttaaaaa
     3061 cagcagatgc tggatattta actagacgtt tggttgacgt atcgcaagat gtaattattc
     3121 aagaagaaga ctgtggtact ttgcgtgggt tagttgctgt ggaattaaaa aacaatgaag
     3181 aagtcgttgc atctttatac gaaagaatat taggtcgcgt ttctgttcat gacatacaac
     3241 atccacttac aggcaaacac attgtatcag caggagaaga aattactgaa aatacagcta
     3301 agataattca agattcgcct attgaacgag tagagattcg ttcggtatta acctgtgagt
     3361 cgaaaaaagg agtttgtgct aagtgttatg gtcgcaattt atcgacaaac agaatggttc
     3421 aaaaaggaga agcagtaggt gtaattgcag ctcaatcaat tggagaacca ggaacacagc
     3481 ttacacttcg aactttccat gttgggggta ttgcctcaaa cgttgctact gaatctaaaa
     3541 taatttcaag atacaatggt attttggaaa ttgaagagtt acgtacagtg agtattactg
     3601 aagaaggtaa gacccaccaa attgtagtaa gtcgtttggc agaattcaaa atcattgagc
     3661 ctactacaaa ggttgttctt acaacacaaa atatcccata tggagccaaa ttgtatttcc
     3721 aatcaggagt aaccgttgaa aaaggggctg taatttgcga atgggatcct tttaatgctg
     3781 ttgttatatc tgaggttgct ggtaaaatga cgttcgaaag tatggttgaa aatattacat
     3841 tcaaaaccga atctgatgag caaactggct tgaaagaacg catcataatt gaatcgaaag
     3901 ataaaagcaa ggttcctgcg gcacatatac ttgatgaaaa aggggaaatt ttacgaacat
     3961 atgcacttcc tgttggtgca cacattgcga ttacagaagg ttctaaaata aaaattggac
     4021 agattttact aaagatgcca cgggcagtag gcaaatctgg agatatcaca ggtggtttgc
     4081 cacgtgtaac agaattgttt gaagcacgta atccatctaa tccagctatt gtctcggaaa
     4141 ttgatggtga aattacattt gggaaaatta agagaggtca gcgcgaaatt gtagttacat
     4201 caaaatctgg agaagtaaaa aaatacttag ttcctctgtc aaaacaaatt ttagttcaag
     4261 aaaatgattt tgtaagagct ggtaatccat tatcagatgg agctattact ccttctgaca
     4321 tattagcaat taaaggacct acggctgtac aagagtatat tgtaaacgaa gttcaggatg
     4381 tatatcgcct acaaggagtt aaaattaacg ataaacactt tgaagtaatc gtgcgtcaaa
     4441 tgatgcgtaa agtggaaata gaagatccag gtgatacacg tttcttagaa aaacaaactg
     4501 ttgacaaaca agagtttatg gaagaaaacg accgtatatg gggtaaaaaa gtagtagtaa
     4561 acccaggtga ttctgaggta ttaatggcag ggcaaattat tgcaccacgt aaattacgcg
     4621 atgaaaacag tatgttgaaa cgtaaagatt taagaacagt tgaggtgcgt gatgctatga
     4681 gtgcaacgtc aagtgctgta ttacaaggta ttactagggc ttcgttacaa actacaagct
     4741 ttatgtcagc agcctcgttc caagaaacta ctaaagtatt aaatgatgct gctataaacg
     4801 gtaaagtgga caagctagaa ggattaaaag aaaacgtgat ttgtggtcat ttaatccctg
     4861 ctggaacagg acaaccagaa ctcga
//
LOCUS       DAOSMY010000086         4860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17529_length_4860_cov_3.308012, whole genome shotgun sequence.
ACCESSION   DAOSMY010000086 DAOSMY010000000
VERSION     DAOSMY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17529_length_4860_cov_3.308012"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLD76_02965"
     CDS             <1..792
                     /locus_tag="PLD76_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOU26997.1"
                     /translation="INELLVTNTENLVDDYGNRSGWIEIYNPTHRVIDIGGCYLTNDT
                     LNPVKYKIPEGDEITLVAPLSHIIFYADNKTHFGIFHTNFTLQESNYIALYDRDQTTC
                     LDSVVYPLTMPANFSYGRLKDGEPEWGMLAKTSPANNNAFVDKGAASKVFAQQDPYGV
                     VLTIASMSVVFLALLLLYTLFSLTAKFSIYVTKERIRKTAKGEKSETIVETHIPAEVY
                     ASIAMAIHEYQEEIHDVEDTILTIQRVAKTYSPWSSKIYGLRNFL"
     gene            886..1332
                     /locus_tag="PLD76_02970"
     CDS             886..1332
                     /locus_tag="PLD76_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOU26998.1"
                     /translation="MKEYNFIINGTKYKVGIQSVEDNIAEVEVNGTPFYVEMEKPLVK
                     KGIKPLAKRAVYTHDETTVSSPSQPVVKPVVKVKTGALLSPLPGVITAISVNVGDTVK
                     IGQKVLALEAMKMENNINADKDGKVLEIKVSKGDSVLEGTELIIIG"
     gene            1337..2500
                     /locus_tag="PLD76_02975"
     CDS             1337..2500
                     /locus_tag="PLD76_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU26999.1"
                     /translation="MIQQDNLLSFLLNSINKIILSTGFANVEIGNIIMIIIGIMFIYL
                     AIKKGWEPMLLVPIGFGMIVGNIPFLEGSDIGIKEEGTVLNILYGGVRYGWYPPLVFL
                     GIGAMTDFASLISNPKLLLIGAAAQFGIFGAYALALAFGFPANEAGSIGIIGGADGPT
                     AIFVTSILSPHLLGAIAVSAYSYMALVPVIQPPVMRLLTTKKERLIHMKKPRAVTQTE
                     KVVFPIMGLLLTTFIVPSGLPLLGMLFFGNLLKESGVTNRLADTAKGPMIDIVTILLG
                     FTVGASTQAAFFLTTKSIFIFMLGAFSFIIATAAGVMFVKIFNLFLKGENKINPLIGN
                     AGVSAVPASARVSHMMGLKYDPTNYLIMHAMGPNVAGVIGSAVAAGIILSFLS"
     gene            complement(2589..3428)
                     /locus_tag="PLD76_02980"
     CDS             complement(2589..3428)
                     /locus_tag="PLD76_02980"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOU27000.1"
                     /translation="MKEISEDSSKVKETPWSIKGNTGINFSQASFVNWVAGGDNSFTG
                     TAYFNVRSSYIQGRSAWNTDLSLEYGGLWLIDNNEYRKNSDKIDFATKYGYAMNKTLF
                     ASGLFDFKTQFSEGYDYKTSPATITSRFVAPAYSKLSIGLDYKPTKVFSLFMSPITGK
                     MTFVSDNSLAIKYGMVDENNVILNPFWFKIEPGAFLKADVNWDITSNINLNTKADFFT
                     AYDNSFGDIDINWDLVVSMKVNQFIRTSISTSLKYDKDILIEGTPKVQFREIIALGIG
                     YSF"
     gene            3501..4262
                     /locus_tag="PLD76_02985"
     CDS             3501..4262
                     /locus_tag="PLD76_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU27001.1"
                     /translation="MIRVGLFGFGKTGKMVAKELLQDDLIELVWVMRRNVISEEKYAS
                     RLIGINENKGMIISKERLTHSFYEEYPVDVIIDFSDASAYKNYAIPASKGIKIISAIS
                     HYSPKALEYIKDLSHQTAIIYSPNITLGVNFLMVISQILQKIAPNADIEIVEEHFREK
                     KGISGTACKMAQILGLDEERHVNSIRVGGIVGKHQVIFGLQNQTIRLIHESISGAAFG
                     KGAIFAAKWIVNKQKGYFSMEQIFSDMIQQNIPVY"
     gene            complement(4266..>4860)
                     /locus_tag="PLD76_02990"
     CDS             complement(4266..>4860)
                     /locus_tag="PLD76_02990"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU27002.1"
                     /translation="LVNFVKHEYGINQVYPKAKNLFNAFNLCPFDSTKVVIIGQDPYH
                     GEGQAHGLCFSVPDHTPIPPSLQNIFKEIKNDLEITPFNTGNLERWAKQGVLLLNATL
                     TVRANVAGSHQGKGWEIFTDKVIQLLSKKKKNLVFILWGSYAQQKKELIDTHNHCIFT
                     STHPSPLSAYRGFFGNKHFSQTNTYLIDHGKEPIDWR"
BASE COUNT         1587 a          710 c          903 g         1660 t
ORIGIN      
        1 ataaatgaac tactagtaac gaatactgag aacttggtgg atgattatgg aaatagaagt
       61 ggttggatag aaatatataa tcctacccat cgtgtgattg atattggggg ttgctatttg
      121 acaaatgata cccttaatcc tgtaaaatac aaaatacctg aaggagatga aataacgctt
      181 gttgctccat tatctcatat aatattttat gcagataata aaactcattt tggaatattt
      241 catacaaact ttacattaca agaaagtaat tatatcgctt tgtatgatag agatcaaaca
      301 acgtgtttag attcggtagt ctatcctctt actatgcctg ctaatttttc ctacggacga
      361 ttgaaagatg gtgaacctga atggggtatg ttggctaaaa catctccagc taataataat
      421 gcctttgtag ataaaggagc tgctagtaag gtttttgcac aacaagatcc atatggagtt
      481 gtattaacta tagcttctat gtctgttgtg tttcttgcac tactactact gtatacatta
      541 ttttctctta cggcaaagtt ttctatatac gtaaccaaag agcgtattcg taaaacagct
      601 aagggagaaa aaagtgaaac aattgttgaa acacatattc cagcagaagt atacgcgtct
      661 attgccatgg ctatacacga atatcaggaa gaaatacatg atgttgaaga tactattttg
      721 actatacaac gtgttgctaa aacgtattct ccttggagtt ctaaaatata tgggttgcgt
      781 aattttttgt aaaaaaaatc aacaagctgt atagaaaaaa taactggtaa atatacaatc
      841 agttatatcg tttactatat atgaattttt taataaataa aatagatgaa agaatataat
      901 tttattataa atggtaccaa atataaagtt ggtatacaaa gcgtagagga caatattgca
      961 gaagtagagg ttaatggcac tcctttttac gtagaaatgg aaaaacctct tgttaaaaaa
     1021 ggtattaaac ctcttgcaaa acgtgcagta tatacgcatg atgaaacaac agtgagctct
     1081 ccttctcaac ccgtagtaaa acctgtagtg aaagtaaaaa caggagcact tttatcccca
     1141 ttacctggag ttattacagc tattagtgtc aatgtaggtg atacagttaa aattgggcaa
     1201 aaagtattag ctctagaggc aatgaaaatg gaaaacaata tcaatgccga taaagatggc
     1261 aaagtattgg aaataaaagt tagtaaaggg gattctgttt tagaaggaac tgaattaatt
     1321 ataataggat agaataatga tacagcaaga taatttactt tcatttttgc tcaatagtat
     1381 caataagatt attttgagta cagggtttgc aaatgtagaa ataggtaata ttatcatgat
     1441 tattatcgga atcatgttta tctatttagc tattaaaaaa ggatgggaac caatgctttt
     1501 agttcctatt ggttttggta tgattgttgg aaacattcct tttttagaag gttcagatat
     1561 tggtataaaa gaagaaggta cagttttaaa tattctttat ggtggggtta gatatggttg
     1621 gtatcctcct ttagtatttt taggtattgg agcaatgact gattttgcat ctcttatctc
     1681 taatccaaaa ttgttattaa ttggagctgc tgcacaattt ggtatatttg gtgcttatgc
     1741 actggcttta gcgtttggat ttcctgctaa tgaagcaggt tcaataggta ttattggtgg
     1801 tgctgatggt ccaacagcta tttttgtaac gtctatatta tccccacatt tattgggagc
     1861 aatagctgtg tcagcctatt cgtatatggc tttagtgcct gttattcaac ccccagtaat
     1921 gaggttgctt acaaccaaga aagagcgtct tattcatatg aaaaaaccaa gggctgttac
     1981 tcagacggaa aaagtggttt ttcctataat gggattatta ttaacaactt ttattgttcc
     2041 atcaggattg ccattattgg ggatgctatt ttttgggaat ttgttaaaag agagtggggt
     2101 aaccaatcga ttagcagata ccgccaaagg tcctatgatt gatattgtta caatcttgtt
     2161 aggttttact gttggagcat ccactcaagc ggcgtttttc ctgacgacta aatcaatttt
     2221 tatttttatg ttgggagctt tttcgtttat tatagctact gctgcaggtg tgatgtttgt
     2281 taagattttt aatttatttt taaaaggcga aaacaaaatc aatccactta ttggtaatgc
     2341 tggtgtttca gctgtgcctg catctgcaag agtttctcat atgatgggtt tgaagtatga
     2401 tcctaccaat tacttgatta tgcacgcaat gggacctaat gtggcaggag tgataggttc
     2461 agcagttgct gcgggtatta ttttaagttt cttgtcataa caagagctta ctatctagtt
     2521 tcagatataa taaaattggg ttgagataga atttctcaac ccaattttat tatagacttt
     2581 ctctttattt aaaaagaata tccaatacct aaggcaataa tttctctaaa ttgcactttt
     2641 ggagtacctt ctattaaaat atctttgtca tatttcaagg aagtactaat acttgtacga
     2701 ataaattgat ttactttcat acttactact aaatcccagt taatgtcaat atcaccaaaa
     2761 ctattgtcat aggcggtaaa gaaatcagct ttggtattta aattgatatt tgacgtaatg
     2821 tcccaattta catcagcttt taaaaaagct cctggttcaa ttttaaacca aaatggatta
     2881 agaataacat tgttttcatc aaccattcca tatttaatgg caagagagtt gtcgcttaca
     2941 aaagtcattt tccctgttat aggagacata aataaagaaa aaacttttgt tggtttataa
     3001 tcaagaccaa tagacagttt tgaatacgct ggtgctacaa atcgtgatgt aatagtagct
     3061 ggagatgtct tataatcata tccttcggaa aattgggttt taaaatcaaa cagtcctgat
     3121 gcaaaaagtg ttttgttcat cgcataaccg tatttagtag caaaatcaat cttatccgaa
     3181 ttttttcggt attcattatt atcaatcaac cataaccccc catactctag tgataaatcg
     3241 gtattccaag ccgaacgtcc ttgaatgtaa gatgatctta cgttgaaata ggcagtgcct
     3301 gtaaaggaat tatccccacc tgctacccaa ttgacaaaac tagcttgaga gaaattaatt
     3361 ccggtatttc cttttatgct ccaaggggtt tcttttactt ttgaagaatc ttcggaaatc
     3421 tctttcatta atttagtgga ttcttttaca tgatcctcta caacatcgag ttgtgcataa
     3481 ctagttgtgt ataaacaacc atgattagag taggactttt tggatttgga aaaacaggaa
     3541 aaatggtggc taaagaatta ttacaagacg atttaattga gctagtatgg gttatgcgaa
     3601 gaaatgttat atcggaagaa aaatatgcca gtcgcttaat tggtataaat gaaaataagg
     3661 gaatgattat ttcgaaagaa agactaacac attctttcta tgaggagtat cctgtagatg
     3721 ttataataga tttttctgat gcaagtgcgt ataaaaatta tgcgatacca gcttcaaagg
     3781 gtataaaaat aatatcagct atatctcatt actcccccaa agcccttgag tatattaaag
     3841 atttgtcaca tcaaacagct attatatatt ctcccaatat tactttaggt gtaaattttt
     3901 tgatggtaat atctcagata ttgcaaaaaa tagcacctaa tgcagatatc gaaattgtag
     3961 aagaacattt tagggagaag aaagggattt caggaactgc ttgcaaaatg gcacaaatct
     4021 tgggtttaga tgaagaacgt catgtaaatt ctattcgagt aggaggtatt gttgggaaac
     4081 atcaagttat atttggctta caaaatcaaa ctattcgtct tattcacgaa agtattagtg
     4141 gtgcggcttt tggtaaaggt gcaatttttg ctgcaaaatg gattgtgaat aaacaaaaag
     4201 gttatttttc gatggagcaa attttttctg acatgataca acaaaatatt cctgtttatt
     4261 aattattatc tccaatctat aggttctttt ccatgatcta taagatatgt attggtttga
     4321 gaaaagtgtt tattgccaaa aaatcctctg taggctgaca agggagaagg atgtgtggat
     4381 gtgaaaatac aatggttgtg agtatcaatt agttcttttt tttgttgtgc gtaactgccc
     4441 catagtataa agactagatt ttttttcttc tttgaaagta gttgaattac tttatcagta
     4501 aagatttccc agccttttcc ttgatgagaa ccagctacat tggcacgtac tgttagagtg
     4561 gcatttaata ataatactcc ttgttttgcc catctttcga ggttgccagt gttaaagggt
     4621 gtaatctcta agtcattttt aatttctttg aagatatttt gtagtgaggg tggtatggga
     4681 gtgtggtctg gaactgaaaa acataaacca tgagcttgtc cttctccatg ataaggatct
     4741 tgacctatta ttaccacttt tgttgaatca aatggacata aattaaaggc attaaataaa
     4801 tttttagctt tgggataaac ttgattgatg ccatattcgt gtttcacaaa gtttactaat
//
LOCUS       DAOSMY010000087         4856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17545_length_4856_cov_2.473443, whole genome shotgun sequence.
ACCESSION   DAOSMY010000087 DAOSMY010000000
VERSION     DAOSMY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17545_length_4856_cov_2.473443"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLD76_02995"
     CDS             complement(<1..287)
                     /locus_tag="PLD76_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27003.1"
                     /translation="MRVLIVCSYKNQYKSHITPFIEEQGYALQSIAKVEVDYFLINGK
                     GILGYLRLLPKLKLKIKEFNPNIIHAHYELSGLLANLQREIPVVTTFLGSD"
     gene            complement(368..1696)
                     /locus_tag="PLD76_03000"
     CDS             complement(368..1696)
                     /locus_tag="PLD76_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27004.1"
                     /translation="MKWQFYVFGLVAVFGFFTYSNQTGKKWSLFSEKLFTKKIFLTSL
                     SIRLIWVIFSYWYYTRVYGDPFDPALPDAMTYHATAAAVADGFWGNYLAEAWNDFVVM
                     WYAPSDMGYQFYLSFVYTIGFKSILFARIIKALLGAYMCILIYKIGTRNFGETVGRVA
                     AILCMLMPNLIYYTGVQLKELEMVFLVVLFIERADYLIRGRHFTVFTVLPPLLIAGSL
                     FFFRTVLGATALFALFTSILFTSKRVMSVNKRIISMVWVIAAVVYFMGGQISNEVQEV
                     WANKDENQVKMNTWRAREDGGNTYAKYATGAVFAPMIFVVPFPTMVSPEKGLQENQQL
                     LHSGYYVKNILGFFIIFSLFTLIKHRKIKFHLLLITFICGYLGVIALSAFAQSERFHL
                     PVLPFLLILAAFGIINTSNKHKKIFNYYLILMCIIIIGWNWLKLAGRGLL"
     gene            complement(1803..2924)
                     /locus_tag="PLD76_03005"
     CDS             complement(1803..2924)
                     /locus_tag="PLD76_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27005.1"
                     /translation="MKILYYIPEQTINDPVIKSQVIDQATFLSKNNYEIFIYAQNTKE
                     HIVEGLIKMFFHNKNHKNPTIKSIITIFQGIKTIKKINPDYVYFRSSITFFFLYLSSF
                     LFKSNLVFDARGVSWEERYMKTKKKNVYYFFLKFTHIFAYKLSHRQLCVTNNFAQLIK
                     KYRKKDDLYVIPSCLSLSENENHLTKLSNDNISRASLGFLETDIIICYSGSTTKWQRI
                     DKICELVNSLNHSNIKLLCMTKEIESMQIIVSEILQSNQFVIKSANNKEELSKYLNLV
                     DFGIIIRDNSLVNMVASPIKIAEYFHAGLPIICNKYIGDIEKILKENPSLGILIDDTN
                     HLLNTILTFKKYNKKMIQSYSIDNYTWNAYLKIFQDCYR"
     gene            complement(2953..3846)
                     /locus_tag="PLD76_03010"
     CDS             complement(2953..3846)
                     /locus_tag="PLD76_03010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU27006.1"
                     /translation="MKIVVVIVTYNGMYWLDKCLGSIRLSTVPLHTLVIDNLSTDNTV
                     AHIQQNYPEVELIKNSYNAGFGQANNMGLRKAIEYDADYVFLLNQDAWIEPTTCQVLV
                     QEHQKKNSIGIISPIHLTGNATELDKNFASYIPYLSLAEVKQLDKSFSVPFVNAAAWM
                     MTRAVIDKVGGFDPLFTHYGEDRDYCQRVKFHGFEIAVVPTVSICHDRKYSANNSYRK
                     QTNLLYSIGLASLKDIRIPFSVAIATYKKNRFKKSIKALLKGDIKRVWNEWTVVHQLL
                     KMKGAIDTSRTMSIKTKMPYL"
     gene            complement(3843..4778)
                     /locus_tag="PLD76_03015"
     CDS             complement(3843..4778)
                     /locus_tag="PLD76_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU27007.1"
                     /translation="MNQPLISIIIPSYNVASYLPDACDSLLAQTYANWEGIIIDDGSA
                     KDNTAEVAQAYCAKDKRFRYIYQENKGLSGARKTGIDAAKGAYIQFLDADDALLPSRL
                     EEMLEIANQADEKTIFYSDFTIGLNNTLKGNLANSSRPHSIGRALHFMDMFTGWRTKF
                     LFVPACPFFPTAIFSTIDYDASLRSVEDWDLYLSILSKGYVFMPMPSKNILYRDNPGG
                     LSKNITYIYTQLFYMLDKWKNTTPVSFKQYDKTIARLYSEIVFMHYWNKNTEIVKPTT
                     ITNKIQFYAYTIAFLVKRVLGIITNAIMKRIKKRP"
BASE COUNT         1671 a          808 c          697 g         1680 t
ORIGIN      
        1 atatcactac caagaaaagt cgtaacaaca ggaatttctc tttgtagatt agctaataat
       61 ccagatagtt cataatgagc gtggataata tttggattaa actctttaat ttttaatttt
      121 aattttggga gaagtcttaa gtagcctaaa atacctttac cattaattaa gaaataatct
      181 acttcaactt ttgcaatact ttgtaaagca taaccttgct cttcaataaa aggggtaatg
      241 tgggatttat attgattttt ataactacaa actattaaaa ctcgcataag aatatttttt
      301 ttttgcaaaa atacaaatta tattatgatt ttatttaata tatatatgtt aatttctaac
      361 agaaattcta taacaatcct ctgcctgcta gttttagcca attccaccct atgattatta
      421 tacacattag tattagataa taattgaata tttttttatg tttgttactt gtattaataa
      481 tgccaaacgc agcaagtatc aacaaaaaag gcaaaacggg caagtgaaat cgttctgatt
      541 gtgcaaacgc acttaaagct ataactccca aatagccaca tataaaagta attaatagta
      601 aatgaaattt aatttttcta tgtttaatta gtgtaaacaa agaaaatata ataaaaaatc
      661 ctaatatgtt ttttacataa tatccactgt gtaataattg ttgattttct tgcaaaccct
      721 tttctggact aaccattgtt gggaatggaa ctacaaaaat cataggagca aatacagctc
      781 ctgttgcata ttttgcatag gtatttcctc catcttctcg agctctccat gtattcattt
      841 taacttgatt ctcatcttta tttgcccata cttcttgcac ttcgttggat atttgacctc
      901 ccataaaata gacaacagcc gctattaccc aaaccatcga aataatgcgc ttgtttacag
      961 acataacccg ttttgacgta aatagtattg acgtaaatag ggcaaacaat gcggtagcac
     1021 ctaatactgt acgaaagaaa aataatgaac ctgcaattaa caatgggggt aataccgtaa
     1081 ataccgtaaa atgacgtcct ctaattaaat aatcggctcg ttcaatgaac agtactacta
     1141 aaaaaaccat ttccaactcc tttagttgaa cgcctgtata ataaattaaa ttgggcatta
     1201 gcatacacaa tatggcggct accctaccta ccgtttcacc aaaattacga gtacctattt
     1261 tgtaaattaa tatacacata taggctccta aaagtgcttt aataatacgt gcaaataaaa
     1321 tagatttaaa ccctattgta taaacaaaag ataaataaaa ttgatatccc atatctgatg
     1381 gggcatacca cataacaaca aaatcgttcc atgcctcggc taaataattc ccccaaaaac
     1441 catcagctac agcagcggca gtggcatggt acgtcattgc atcaggaaga gctggatcaa
     1501 aggggtcgcc atatactctg gtataatacc aataactaaa aataacccaa atcaaacgaa
     1561 tagacaagct ggttaaaaat atcttttttg taaataattt ttctgaaaaa aggctccatt
     1621 tcttgcctgt ttgattcgaa tatgtaaaaa atccaaaaac agcaaccaat ccaaaaacat
     1681 aaaattgcca tttcattgag aatttaaaaa aaaacataaa aataagcacc attgcagtta
     1741 tatacatgct aatagcccta gaaccgaata tttttggaaa ataactaaac ataaaataaa
     1801 tattatctat aacaatcttg aaaaatttta agatatgcat tccatgtgta attatctatt
     1861 gaatatgatt gaatcatctt tttgttatac tttttaaaag ttagaattgt atttaacaaa
     1921 tgattagtat catcaataag tattcctaaa ctaggattct ctttaagtat tttttcaata
     1981 tctccaatat atttattaca aattattggt agccctgcat gaaagtattc agcaatttta
     2041 ataggagaag caaccatatt tactaaggaa ttatctctga taattatacc aaaatcaact
     2101 aaattgagat attttgagag ttcttcttta ttattagccg attttataac aaattgattt
     2161 gattgtaaaa tttcacttac aattatttgc atactttcaa tttcttttgt catacataac
     2221 aacttaatgt ttgaatgatt taaagaattt acaagttcac atattttatc tattctttgc
     2281 cattttgttg tagaaccact ataacatatt ataatatctg tttctaaaaa tcctaaagaa
     2341 gctcttgaaa tattatcatt ggatagtttt gtgagatgat tctcgttttc agataatgat
     2401 aaacatgaag gtattacata taaatcatcc ttttttctat actttttaat taactgtgca
     2461 aaattattag taacacaaag ctgtctatgt gataatttat aagcaaaaat atgagtaaat
     2521 tttaagaaaa agtaatatac attctttttc ttcgttttca tatatctttc ttcccacgaa
     2581 actcctcgtg catcaaacac taaatttgat ttaaataaaa aagaagataa ataaagaaag
     2641 aagaaggtta ttgaagacct aaaataaaca taatctgggt taattttttt tatcgtcttt
     2701 ataccttgaa aaattgtaat tatagacttt attgtaggat tcttatgatt cttattatga
     2761 aaaaacattt taattagacc ttcaacaata tgttcttttg tattttgagc atatataaat
     2821 atctcgtaat tatttttaga aagaaaagtt gcctgatcaa taacttgact tttaataact
     2881 gggtcattaa tggtttgctc tggtatgtaa tatagtattt tcataattaa tataacggta
     2941 taataatatg ggtcataaat aaggcatttt ggtctttata ctcatcgttc tgctagtgtc
     3001 aattgctcct ttcattttga gcaattgatg taccacagtc cattcattcc atactcgttt
     3061 tatatctccc tttaataagg cttttatgct ttttttgaaa cgattctttt tgtatgtcgc
     3121 aatagcaaca gaaaaaggaa tacgtatgtc ttttaacgag gctaaaccaa tagaatataa
     3181 taggtttgtt tgtttgcgat aagaattatt ggcactgtac tttcggtcgt gacaaatgct
     3241 taccgttggt actacagcaa tctcaaatcc atggaatttt actctctggc agtaatctct
     3301 atcttcgcca tagtgcgtaa acaaagggtc gaacccccct actttatcaa tcactgcacg
     3361 tgtcatcatc catgccgcag catttacaaa tggtacggaa aaacttttgt caagttgttt
     3421 aacctcagca agtgataaat atggaatata cgaggcaaaa tttttatcca attcagtagc
     3481 attgcccgta agatgaatgg gagatataat ccctattgag ttttttttct gatgttcctg
     3541 taccaatact tggcaagtag tgggctctat ccaagcgtct tgatttaata aaaatacata
     3601 atcggcatca tattctatgg ctttgcgtaa gcccatgtta ttggcttgtc caaaacctgc
     3661 attatacgag ttttttatca attctacttc tgggtagttt tgttgtatat gtgcaacggt
     3721 attatcggta gataaattat caataaccaa ggtatgtagc ggtactgttg ataaacgaat
     3781 acttcccagg catttatcca accagtacat tccattgtat gtaacaataa ctactacaat
     3841 tttcatgggc gtttttttat tcgtttcatt atagcatttg taataatacc cagcacacgt
     3901 ttcacaagaa atgctattgt ataggcataa aactgaattt tattggtaat agttgtgggt
     3961 tttacaattt ctgtattttt attccaatag tgcataaata ctatttcgct atacaagcgt
     4021 gcgatggttt tatcgtattg tttaaaagat acaggcgttg tgtttttcca tttatctagc
     4081 atataaaaca actgcgtgta aatataggtt atatttttag acaaccctcc tggattatct
     4141 cggtacagaa tatttttcga tggcattggc ataaacacat atcctttgct taggatgctt
     4201 aaatataaat cccaatcttc caccgaacgc aacgaagcat cgtagtcaat agttgaaaaa
     4261 atagccgtag gaaaaaatgg acacgcaggc acaaacaaga atttggttcg ccatcctgta
     4321 aacatatcca taaagtgtaa tgccctacca atgctatgag gtcgagatga attcgctaag
     4381 ttgcctttta aggtattatt caacccaatg gtaaaatcag aataaaaaat tgttttttca
     4441 tctgcttgat tcgctatttc tagcatttct tccaaacggg atggaagtaa agcatcgtcg
     4501 gcatctaaaa actgaatata cgcaccttta gctgcatcaa tacctgtttt ccgagctccc
     4561 gaaagtcctt tattttcttg ataaatgtat ctgaaacgtt tatctttagc acaatatgcc
     4621 tgtgctacct cagcagtatt atctttggct gaaccatcat ctataataat tccttcccag
     4681 ttggcgtagg tttgagccaa taagctatca cacgcatcag gcaaataaga tgctacattg
     4741 tatgatggta taataattga aatgagtggt tgattcataa tttcagctct taataaattt
     4801 aatgcaaagg tagtaaatat taaagcttaa agcaattttg tatttaactt tttaaa
//
LOCUS       DAOSMY010000088         4791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17816_length_4791_cov_3.374155, whole genome shotgun sequence.
ACCESSION   DAOSMY010000088 DAOSMY010000000
VERSION     DAOSMY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17816_length_4791_cov_3.374155"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..499
                     /gene="rplV"
                     /locus_tag="PLD76_03020"
     CDS             89..499
                     /gene="rplV"
                     /locus_tag="PLD76_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU27008.1"
                     /translation="MGARKKISAEENKEALKGLYFAKLNNVPTSPRKMRLVADMIRGV
                     EVHKALGLLKFSTKEAAGRLEKLLRSAISNWEQKTEQKAEDANLIVSRIHVDSGRMLK
                     RLRPAPQGRGYRIRKRSNHVTIYVDTVNTNDTKN"
     gene            506..1243
                     /gene="rpsC"
                     /locus_tag="PLD76_03025"
     CDS             506..1243
                     /gene="rpsC"
                     /locus_tag="PLD76_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOU27009.1"
                     /translation="MGQKVNPISNRLGIIKGWDSNWYGGNNYGDTILEDFKLRKYLEA
                     RLAKASVSRIIIERTLKLITITVCTARPGIIIGKGGQEVDKLKEELKKITDKDVQINI
                     FEIKKPEVDAVIVANNVARQIEGKIAYRRAVKMAIASTMRAGAEGIKIMVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYAHAEALTKVGIIGVKVWICRGEIYGKKDLTPQFAT
                     QKEGARPQFGNANRSENAGKGFKKRKK"
     gene            1272..1706
                     /gene="rplP"
                     /locus_tag="PLD76_03030"
     CDS             1272..1706
                     /gene="rplP"
                     /locus_tag="PLD76_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU27010.1"
                     /translation="MLQPKKTKFRRQQKGRMKGNAQRGNQLAFGSFGIKSLESKWITG
                     RQIEAARIAVTRYMQRQGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAPITPGRV
                     IIEVEGVPFEIAKEALRLAAQKLPVTTKFVTRRDYDYESPNA"
     gene            1713..1910
                     /gene="rpmC"
                     /locus_tag="PLD76_03035"
     CDS             1713..1910
                     /gene="rpmC"
                     /locus_tag="PLD76_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOU27011.1"
                     /translation="MKIAEVKELSNQEIIERIDGEKEKLSQLKLNHSISPLDNPNQIK
                     EVRKTIARLNTELRAREMKQN"
     gene            1923..2174
                     /gene="rpsQ"
                     /locus_tag="PLD76_03040"
     CDS             1923..2174
                     /gene="rpsQ"
                     /locus_tag="PLD76_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU27012.1"
                     /translation="MRNLRKERQGVVFSNKMDKTITVAIKWKEKHPIYGKFVNKTKKY
                     HAHDEKNECTIGDTVKIMETRPLSKLKRWRLVEIIERAK"
     gene            2176..2541
                     /gene="rplN"
                     /locus_tag="PLD76_03045"
     CDS             2176..2541
                     /gene="rplN"
                     /locus_tag="PLD76_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU27013.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGGTGKRYATLGDVIVVAVK
                     SVIPSSDVKKGAVSKAIIVRTKKEVRRADGSYIRFDDNACVLLNAAGEMRGSRIFGPV
                     ARELRTANMKIVSLAPEVL"
     gene            2562..2876
                     /gene="rplX"
                     /locus_tag="PLD76_03050"
     CDS             2562..2876
                     /gene="rplX"
                     /locus_tag="PLD76_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU27014.1"
                     /translation="MTKLHIKKGDTVYVNAGDDKGTVARVLEVLVKEQRAIVEGVNMV
                     SKHTKPNAKNPQGGIVKKEASIHISNLNPTVKGDKPTRVGRKQVDGKLVRYAKKTGEE
                     IK"
     gene            2876..3421
                     /gene="rplE"
                     /locus_tag="PLD76_03055"
     CDS             2876..3421
                     /gene="rplE"
                     /locus_tag="PLD76_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU27015.1"
                     /translation="MSNKASLKKEYTERIAPALKKEFGYKSAMQVPVLEKIVLNQGLG
                     IAVADKKIIEIAINEMTAIAGQKAIATYSKKDISNFKLRKKMPIGVRVTLRREKMYEF
                     LERLVRVALPRIRDFKGIDSKLDGRGNYTLGIQEQIIFPEINIDSINKILGMNITFVT
                     SAKTDEEAYALLKEFGLPFKK"
     gene            3442..3711
                     /gene="rpsN"
                     /locus_tag="PLD76_03060"
     CDS             3442..3711
                     /gene="rpsN"
                     /locus_tag="PLD76_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOU27016.1"
                     /translation="MAKESMKAREVKRAKMVAKYAEKRTQLKADGDYEALQSIPKNAS
                     PVRLHNRCKITGRPKGYMRIFGISRIQFREMASKGLIPGVRKASW"
     gene            3786..4181
                     /gene="rpsH"
                     /locus_tag="PLD76_03065"
     CDS             3786..4181
                     /gene="rpsH"
                     /locus_tag="PLD76_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU27017.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEIPASNLKKELTKILLDKGYI
                     LNYKFEETGHQGTIKIALKYDSVNKVNAIKKLIRVSTPGLRKYVGHTDLPRVLNGLGI
                     AIISTSKGVMTDKEAREMNVGGEVLCYVY"
     gene            4204..4758
                     /gene="rplF"
                     /locus_tag="PLD76_03070"
     CDS             4204..4758
                     /gene="rplF"
                     /locus_tag="PLD76_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU27018.1"
                     /translation="MSRIGKLPITLPKGVTVTFANNVVTVKGPKGELQQTVHPNIMVA
                     IDNGVVTVARPNDDKENRAMHGLYRSLLNNMVIGVSEGYKQTLELVGVGYRVSNNGQI
                     LEFSLGYTHGIFLKLPNEIKVETKSERNQNPIVILESCDKQLIGQVCAKIRSFRKPEP
                     YKGKGVKFAGEQVRRKAGKSAGAK"
BASE COUNT         1832 a          674 c          977 g         1308 t
ORIGIN      
        1 ttcgctaact cgtaatttta gaggtcatgg tggaaataaa aagaaataac cgttgaatat
       61 aaattaggaa taaataataa gtataaaaat gggtgctaga aaaaaaatat cagcagaaga
      121 aaataaagaa gctttaaagg gtctttattt cgcaaagcta aataatgtgc ctacgtctcc
      181 acgtaaaatg cgtttagttg cagatatgat aagaggcgtg gaagtgcaca aagcgcttgg
      241 actgttaaaa ttttccacca aagaagctgc cggtcgttta gaaaaacttc ttcgctctgc
      301 aatttctaat tgggaacaaa aaacagaaca aaaagcagaa gatgctaact taatagttag
      361 tcgcatacat gtggattcag gtagaatgct taaaagactt cgtccagctc cgcaaggtag
      421 aggatacaga attcgtaaac gctcgaacca tgtaaccatt tatgtagaca ctgtaaatac
      481 taacgataca aaaaattaat agtagatggg acaaaaagta aatccaataa gtaaccgttt
      541 aggaatcatc aaaggatggg attctaattg gtatggtgga aataactatg gtgatacaat
      601 tttagaagat tttaaactta gaaaatattt agaagctcgc ttagctaagg ctagtgtttc
      661 tcgtatcatt attgaacgca cactaaaact catcactatt acagtatgta cagctcgtcc
      721 tggtatcata attggtaaag gtggtcaaga ggttgataaa ctcaaagaag agcttaaaaa
      781 aattactgat aaagatgttc aaatcaatat ttttgagatc aaaaaaccag aggtagatgc
      841 agtgattgtt gcaaataatg ttgctcgtca aatagagggt aaaatcgcct atagaagagc
      901 agttaaaatg gcaattgcat caactatgcg tgcaggagca gagggaatta agataatggt
      961 ttctggtcgt ttaaatggtg cagagatggc tcgttcggaa atgtataaag aaggtagaac
     1021 tcctttacat acttttagag cagatattga ctatgcacat gctgaagctt taacaaaagt
     1081 aggtataatt ggcgttaaag tgtggatttg tcgtggcgaa atatatggta aaaaagattt
     1141 gacaccgcaa tttgcaaccc aaaaagaagg tgctcgtcct caatttggaa atgccaatcg
     1201 atctgaaaac gcaggcaaag gttttaaaaa aagaaaaaaa taatctgtta acgaatttta
     1261 cataaggaaa catgttacaa cctaaaaaaa caaagttcag aagacagcaa aaaggccgta
     1321 tgaagggtaa tgcccaacgt ggcaatcagc tggcattcgg atctttcgga ataaagtctt
     1381 tagaatccaa atggattaca ggaaggcaaa tagaagctgc tcgtattgca gttacgcgtt
     1441 atatgcagag acaaggtcaa atttggatac gtatttttcc agataaacca ataactaaaa
     1501 aacctgctga ggtgcgtatg ggtaaaggaa aaggagcccc tgagggtttt gttgccccga
     1561 ttacacctgg tcgtgtaatt attgaagtgg aaggtgttcc tttcgaaatt gctaaagaag
     1621 cattacgctt agcagcacaa aaattgccag taactaccaa atttgttact agacgtgatt
     1681 atgattatga atctcctaat gcataatgag gtatgaaaat agctgaagta aaggaattat
     1741 ccaaccaaga gattatcgaa agaattgatg gcgaaaaaga aaaattatct caattaaaat
     1801 tgaatcatag tatttctcca ttagataatc caaatcaaat taaagaagtc cgtaaaacaa
     1861 ttgctcgtct gaacacagaa ctacgtgcaa gagaaatgaa acaaaattaa acttgacctg
     1921 atatgagaaa tttaagaaaa gaaagacagg gcgtggtttt tagcaataaa atggataaaa
     1981 ccattactgt tgctatcaag tggaaagaaa agcatcctat ttacggtaag ttcgtgaata
     2041 aaacaaaaaa ataccatgct catgatgaaa aaaatgagtg tacaattggt gatacagtaa
     2101 aaattatgga aactcgtcct ttaagcaagc ttaaaagatg gagattagta gaaataatcg
     2161 aaagagcaaa ataacatgat acaacaagaa agtagactga atgtcgcaga caatagcggt
     2221 gcaaaagaag ttctttgtat ccgagtatta ggaggaacag gtaaaagata tgctacactt
     2281 ggagatgtta ttgttgtagc ggtaaaatct gtaattccat caagtgatgt aaaaaaaggt
     2341 gctgtttcta aagcaatcat tgtacgtacc aaaaaagaag tacgtagagc cgacgggtct
     2401 tatatccgtt ttgatgacaa cgcatgtgtg ttgttaaatg ctgctggtga aatgagaggt
     2461 agtcgtatat ttggtcccgt agcacgtgaa ttgcgtacag caaatatgaa aattgtatcg
     2521 cttgcaccag aagttttata accctttgaa caatatacaa aatgactaaa ttacacataa
     2581 aaaaaggaga caccgtttac gtaaatgcag gtgatgacaa gggtacagtc gctcgtgtgc
     2641 ttgaggtttt ggtaaaagaa caaagagcta tcgtagaagg tgttaatatg gtgtctaagc
     2701 acactaaacc aaatgcaaaa aatccacaag gtggtattgt taaaaaagag gcgtctatcc
     2761 atatttcaaa cttaaatcct actgtaaaag gagataaacc tacacgagta ggacgcaaac
     2821 aagttgatgg taaattggta cgttacgcta aaaaaactgg ggaggaaatt aaataatgag
     2881 taacaaagca agtcttaaaa aagagtatac agagcgtatt gctcctgctt taaagaaaga
     2941 gtttggatac aaatctgcaa tgcaggttcc agttcttgaa aaaatagttt tgaatcaagg
     3001 tttaggtata gccgtagctg ataaaaaaat tattgaaatt gcaattaatg aaatgactgc
     3061 tattgctggt caaaaagcta tagctaccta ttcaaaaaag gatatttcta attttaaatt
     3121 aagaaaaaaa atgcctattg gtgttagggt aactttaaga agagaaaaaa tgtatgaatt
     3181 tctcgaaaga cttgttcgtg tagctttacc tcgtatacgt gattttaaag gtattgatag
     3241 taaacttgat ggaaggggta attatacgct aggtatccaa gaacaaatta tttttcctga
     3301 aataaatatt gattcaatca ataaaatatt gggaatgaat attacctttg taacatctgc
     3361 aaaaacagat gaggaagcgt atgcattact aaaagaattt ggattacctt ttaaaaaata
     3421 attgagcaaa aatactagaa tatggcaaaa gaatcaatga aagctcgcga ggtaaaaaga
     3481 gcgaagatgg tagctaaata tgctgaaaaa agaacacaat tgaaagctga tggcgattat
     3541 gaagcattac aaagtatacc aaaaaatgct tctccagtaa gattacacaa tcgttgcaaa
     3601 attactggaa gaccaaaagg atatatgaga atatttggta tttctcgtat ccaatttcgc
     3661 gaaatggcat caaaaggttt aataccaggt gtacgtaagg caagttggta attaataaac
     3721 gaataactaa tttcaatttt taaatattgt ccggacggtc cggattaatt taactttaat
     3781 aaaatatgac agatccaatt gcagattatt tgacacggtt aagaaatgcg attaaagcaa
     3841 atcacagagt agtagaaatc cccgcgtcga atttaaaaaa agagcttacc aagatattgt
     3901 tggataaagg ctatatcttg aattataagt tcgaagaaac aggtcatcaa ggtactatta
     3961 aaattgcttt gaaatatgac tcagttaata aggtaaacgc aataaagaaa cttatcagag
     4021 tttcaacgcc tggtttacgt aagtatgttg gacatactga tttgccaaga gttttaaatg
     4081 gtttaggcat tgcaattatt tctacttcaa agggagttat gacagacaag gaagctcgtg
     4141 aaatgaatgt tggtggagaa gttttatgtt atgtgtatta atttaatttg aggaggatta
     4201 acaatgtcta gaataggaaa attaccgata acattaccca aaggggtaac tgttactttt
     4261 gcaaataatg ttgtaactgt aaaaggtcct aaaggggaac ttcaacaaac tgttcatcca
     4321 aatattatgg ttgccataga taacggagta gtaaccgttg ctagacctaa tgatgacaaa
     4381 gaaaatagag ctatgcatgg attgtatcgt tctttgctta ataatatggt aataggtgtc
     4441 tcagaaggat ataaacaaac actagaatta gttggtgtgg gatatcgtgt ttctaataat
     4501 ggtcaaatac ttgaattttc attaggttat actcacggaa tctttctaaa gcttccaaat
     4561 gaaataaaag tggaaactaa aagtgagaga aaccaaaacc ctatcgttat attagagtca
     4621 tgtgacaaac aattgatagg tcaggtatgt gcaaaaatac gttcattccg taaacctgaa
     4681 ccttataaag gaaaaggtgt taagtttgca ggtgaacaag ttcgcagaaa ggctggtaaa
     4741 tcagcaggtg ctaagtaatt ttaaaaggaa acaatcatga cagaaaaaat a
//
LOCUS       DAOSMY010000089         4782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17855_length_4782_cov_3.629575, whole genome shotgun sequence.
ACCESSION   DAOSMY010000089 DAOSMY010000000
VERSION     DAOSMY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17855_length_4782_cov_3.629575"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLD76_03075"
     CDS             complement(<1..377)
                     /locus_tag="PLD76_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOU27019.1"
                     /translation="MNKIISKEHFSANVVKLEVEAPMIAKSRRAGHFVLVKVGEKGER
                     IPLTITSSDLEKGTITLVIQKMGVSSTKICNLNEGDYITDLVGPLGKATHIEKFGTVV
                     CAGGGVGLAPLLPIVEALKKAGNR"
     gene            complement(479..1813)
                     /locus_tag="PLD76_03080"
     CDS             complement(479..1813)
                     /locus_tag="PLD76_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU27020.1"
                     /translation="MNAKNVIENLKKRFPNEPEYLQAVEEVLESIEEVYNQHPEFEKA
                     NLIERLCIPDRIFTFRVSWVDDKGNVQVNMAYRVQHNMAIGPYKGGIRFHSSVNPSIL
                     KFLAFEQTFKNALTTLPMGGGKGGSDFHPRGKSSGEVMRFCQAYLLELHRHIGPHTDV
                     PAGDIGVGGREVAYMFGMYKKLSHEFTGTFTGKGIEFGGSLIRPEATGYGNIYFLLNM
                     LATRNIDIKGKKCLVSGSGNVAQYTCEKLIELGAIPVTLSDSNGYIYDPEGITREKLE
                     YVMDLKTVQRGRIKEYADKYNCKYVEGGRVWAEKGDIALPSATQNELNGDDAKTLMAN
                     GCIAVSEGANMPSTPEAIEIFLKNKILYGPGKAANAGGVATSGLEMSQNAIGLSWSRE
                     EVDARLKGIMNNIHENCVKYGTEKDGFVNYVKGANIAGFMKVAKAMMAQGIV"
     gene            2187..>4782
                     /locus_tag="PLD76_03085"
     CDS             2187..>4782
                     /locus_tag="PLD76_03085"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU27021.1"
                     /translation="MELASKYNPSEIESKWYQYWIDNALFESKPDNREPYTIVIPPPN
                     VTGVLHMGHILNNTIQDILIRRARMQGKNALWVPGTDHASIATEAKVVNKLAEEGIKK
                     TDLTRDEFLKHAWDWTEKHGGIILHQLRKLGASCDWSRTAFTMDEERSISVLKVFCDL
                     FEKGKIYRGVRMVNWDPQALTALSDEEVIFKEQQGKLYYLKYKVVGEDAYAVVATTRP
                     ETIMGDTAMCIHPNDAKNAFLKGKKVIVPLINREIPVIEDEYVDAEFGTGCLKVTPAH
                     DVNDYMLGEKYNLPSIDIFNDNGTLNEHGCQYTGMDRFDVRNKIEQDLLAAGLLEKVE
                     PYTNKVGYSERTNVPIEPKLSMQWFLKMEDIAKPALDAVMTDDVKFYPAKYKNTYRHW
                     MENIKDWCISRQLWWGHRIPAYYLPEGGYVVALTEQEALEKAQKMNPSLTLADLRQDE
                     DCLDTWFSSWLWPISLFNGITEPNNEEIKYYYPTSDLVTGPDIIFFWVARMIMAGYEY
                     ENKQPFNHVYFTGIVRDKLGRKMSKSLGNSPDPLELIERFGADGVRMGMMLAAPAGND
                     ILFDESLCEQGRNFNNKIWNAFRLVKGWEVADIPQPESAVVAIKWFKAQLAKTITEVD
                     DLFSKYRISEALMAVYKLFWDEFSAWYLEMVKPAYQQPIDKVTYEATLQFFEELLLVL
                     HPFMPFITEELWQSIYTRQDGESIVVQLQSKASEYDEELIVRFEKIKEIIAGIRTIRV
                     QKNIPNKETLPLQIVGEHNSFFDLILLKLGNLRSIEHVNEKTDTSVAFLVGTTEYAIV
                     LGNLINIEDEIKKIETEIRYLEGFLQSVVNKLSNERFVANAKPEVVDAERKKQLDAES
                     KISTLREAL"
BASE COUNT         1519 a          819 c          882 g         1562 t
ORIGIN      
        1 cggtttcctg ctttttttaa tgcttctacg ataggtagaa gtggggcaag acctacacca
       61 ccaccagcac atacaactgt gccaaatttt tcgatatgag ttgctttgcc taatggtcct
      121 acaaggtctg ttatataatc accttcgtta agattacata ttttggtaga tgaaactccc
      181 atcttttgta ttaccaatgt tattgtccct ttttctaaat cagacgatgt gattgttaac
      241 ggaatgcgtt cccctttttc tcccactttg actagtacaa aatgtccagc acgacgtgat
      301 ttagctatca tcggtgcttc aacttctaac tttactacat tggcagagaa gtgttcttta
      361 ctaattattt tattcattct ataaaaatta aaagatttta aacagcaaaa cattgcacat
      421 tatcaatatc ttgaatatcg acaacgtgca atgaaatata taaacaagta tgtgtactct
      481 atacaatacc ttgagccatc atagctttag ctactttcat aaagcctgct atatttgctc
      541 ctttcacata attaacaaaa ccgtcttttt ctgttccgta ttttacacag ttttcgtgaa
      601 tgttgttcat aatacctttc aaacgggcat caacttcttc tctcgaccag ctcaatccga
      661 tagcattttg agacatttct aaaccagaag tagccactcc tcctgcattg gctgcttttc
      721 caggtccata aagaatcttg tttttcaaga atatctcaat cgcttctgga gttgaaggca
      781 tatttgctcc ttctgataca gcaatacaac cgtttgccat aagagttttt gcatcgtcgc
      841 cattcaattc gttttgagta gctgatggta aagcgatatc acctttttca gcccatacac
      901 gaccaccttc tacgtattta caattgtatt tgtcggcata ttctttaata cgtccacgtt
      961 gtactgtttt taaatccatt acgtattcta atttttcacg tgtaatccct tctggatcgt
     1021 agatataacc gtttgaatca gatagtgtta caggaattgc tcccaattca attaattttt
     1081 cacaagtata ttgagctacg ttacccgaac cagatactaa acatttttta cctttaatat
     1141 caatgttgcg tgtagctaac atatttaata agaagtaaat atttccgtaa ccagttgctt
     1201 ctggacgaat taatgaacca ccaaactcaa ttcctttacc tgtaaaagtg ccagtaaatt
     1261 cgtgagaaag ttttttgtac ataccaaaca tataagcaac ctcgcgtcca cctacaccta
     1321 tgtcaccagc aggtacgtca gtgtgaggac caatatgacg atgaagttcc aataaataag
     1381 cttgacaaaa acgcataacc tcgccagatg attttcctct tgggtggaaa tcagaacctc
     1441 ctttaccacc acccattggt agtgttgtaa gtgcattttt gaacgtttgc tcaaacgcta
     1501 aaaacttcaa aatcgatgga tttacagaag agtggaaacg aattcctcct ttgtaaggtc
     1561 caatagccat gttgtgttgt acgcgataag ccatgtttac ttgaacattg cctttatcgt
     1621 caacccacga tactctaaat gtaaagattc tatcaggaat acacaatctt tcaattaagt
     1681 ttgctttttc aaactcagga tgttggttgt acacttcttc gatagattct agtacttctt
     1741 ccaccgcctg caagtattca ggctcatttg gaaatctttt tttaagattt tcaataacgt
     1801 tttttgcgtt cataaattaa atgtatttat aaaaattttt ttctactcgg acgcaaaaat
     1861 acacatttta tcgttttgac aaaataaatg tttcgttttt tgttgaatgt tttccttgtt
     1921 tgtgttgaaa tggttttaaa tcatcctgta accttttaaa atggaattaa tattgtttta
     1981 ttagtttgaa aatgaaaata gtagctatgt gttgttttat ttattgctaa caaaatgtca
     2041 gttattaact tgtgctcagt tattattgca tgcatttcat tcttttactt gattattcat
     2101 cttatatatt ctatcaattt gttgtaagtt ttttttaact ttgcaccttc aaaattatat
     2161 acattgaata cataattatt ttatatatgg aattagccag taagtataat ccttcagaga
     2221 ttgaatcaaa atggtatcaa tattggatag acaatgcttt gtttgaatcc aaaccagata
     2281 atagggaacc ctatactatc gttatcccac ctcctaacgt tactggagtg ttgcacatgg
     2341 gacatatttt gaataatacg attcaagata ttttgatacg cagagcacgt atgcaaggga
     2401 aaaacgcttt gtgggtgcct ggcactgacc atgcttctat tgcaactgaa gcaaaagttg
     2461 taaataaact ggctgaagaa gggataaaaa aaactgattt aacacgcgat gagtttttga
     2521 aacacgcatg ggattggaca gaaaaacacg ggggaattat tttacatcaa ttacgtaagt
     2581 tgggagcttc gtgcgattgg agcagaacgg cttttacgat ggatgaggag cgttcgataa
     2641 gtgtactcaa ggttttttgc gatttatttg aaaaagggaa aatttatcgt ggagtacgca
     2701 tggtaaattg ggatccacaa gctcttactg ccttgtctga tgaagaggtt atttttaaag
     2761 aacaacaagg taaattatat tacttaaagt ataaagttgt gggagaagat gcctatgcag
     2821 tagtagctac aacacgtccc gaaaccatta tgggagatac ggcaatgtgt attcatccaa
     2881 atgatgccaa aaatgcattc ctgaaaggga aaaaagtgat tgttcctttg attaatagag
     2941 aaatacctgt tattgaagat gaatacgtag atgctgaatt tggtactggt tgtttgaaag
     3001 taacgccagc acatgatgta aacgactaca tgctaggtga aaaatataat ttgccaagta
     3061 tagatatttt taatgacaat ggcaccttaa atgaacatgg ctgtcagtat actggtatgg
     3121 ataggtttga tgtgcgaaat aagatagaac aagatttgct tgcagctggt ttgttagaaa
     3181 aagttgaacc atataccaat aaggtaggtt attcagaacg aaccaatgtt cctattgagc
     3241 ccaaattatc gatgcaatgg tttttgaaaa tggaagacat agccaagcct gcattagatg
     3301 ccgttatgac tgatgacgta aagttttatc cagccaaata caaaaatacg tatcgccact
     3361 ggatggaaaa tatcaaagat tggtgtataa gtcgccaact ttggtgggga caccgcatac
     3421 ctgcatatta cttacctgaa ggaggatatg tggtagcatt aaccgaacaa gaggctttgg
     3481 aaaaagccca aaaaatgaat ccatcgttaa ctcttgctga tttacgtcaa gatgaagatt
     3541 gcctagacac gtggttttct tcgtggcttt ggcccatatc tctgtttaat ggtattacag
     3601 agccgaataa tgaagaaata aaatattatt atcctactag tgatttagtt accggacctg
     3661 atattatttt cttttgggta gctcgtatga ttatggcagg ttatgaatat gaaaacaaac
     3721 agccttttaa tcatgtctat tttacaggga ttgttcgtga taagttaggt agaaaaatgt
     3781 ctaaatcatt aggtaattcg ccagacccat tggagcttat tgaacgattc ggtgccgatg
     3841 gtgtgcgtat gggaatgatg cttgcagcac ctgcaggtaa tgatatattg tttgacgaat
     3901 cgttatgcga acaaggaaga aattttaata ataaaatttg gaatgcattt cgattagtaa
     3961 aagggtggga ggttgctgat attccacagc cagaatccgc tgtagttgct attaaatggt
     4021 ttaaggcaca gttagctaaa acaataacag aagtagatga tttattttcc aagtatcgga
     4081 tttctgaagc attaatggct gtatataagt tgttttggga tgaattctct gcctggtatt
     4141 tagagatggt gaagcccgct tatcaacaac cgatagataa ggtaacatac gaagctactt
     4201 tgcaattttt tgaagaatta ttattggttt tacatccttt tatgccattt attactgagg
     4261 aattgtggca atcgatatac acgaggcaag atggagaaag tattgtggta caattacaat
     4321 cgaaagcatc cgaatatgat gaagaattaa ttgtacgttt tgaaaaaata aaagaaataa
     4381 ttgcaggcat tcgtactatt cgtgtgcaaa aaaatatccc gaacaaagaa accttgccat
     4441 tacaaattgt cggtgagcac aattcgtttt ttgatttaat acttctaaaa ctaggcaatt
     4501 tacgttctat tgagcatgta aatgaaaaaa cagatacttc tgtggctttt ctggttggca
     4561 ctacagaata tgccattgtg ttgggaaatt taataaatat tgaagatgaa atcaaaaaaa
     4621 ttgaaacaga aatacgctat ttagaaggat ttttgcaatc tgtagttaat aaacttagca
     4681 atgaaagatt tgtagcaaat gctaaacccg aagtggtaga cgcagaacgc aaaaaacaac
     4741 tagacgcaga aagtaaaatc tccactttac gtgaggcatt ac
//
LOCUS       DAOSMY010000090         4764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17939_length_4764_cov_3.982162, whole genome shotgun sequence.
ACCESSION   DAOSMY010000090 DAOSMY010000000
VERSION     DAOSMY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17939_length_4764_cov_3.982162"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLD76_03090"
     CDS             complement(<1..910)
                     /locus_tag="PLD76_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27022.1"
                     /translation="MVLVFTDKYTPRVKYIFEQIFKEILHVDISFTTKEQVAIDSTIP
                     VICYSREHRIPKSFVIEPEGLLFKRGIKDIQPEVTEWNGTKIFFQSQSADVSIPFDIF
                     AASFYLLTRYEEYGSLDLDEHGRYKAENSLAYKHQFLDYPLVDHWAYLLDQMLADYFP
                     MYTIKSKSRFRFCPSIDIDHVYKYKSKFLSINIIRLFQKLITGKYEDFRYHLNVILNR
                     APDPYFNVEYIVDMHNDYNLRPIFFLHVGPMGKYDGYSLYNPFNIIKDLFYRTLIVGL
                     HPSYFASTNLHQMKKERPDKEYIIVPK"
     gene            complement(921..1616)
                     /gene="radC"
                     /locus_tag="PLD76_03095"
     CDS             complement(921..1616)
                     /gene="radC"
                     /locus_tag="PLD76_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU27023.1"
                     /translation="METKLLSIKEWSQEDRPREKLLEKGIAALSDAELIAILIGSGTR
                     NETAVELAKKILLLANNDLNQLSRLSVKELTKSTKGVGNAKAISIVAALELGRRRKRI
                     DANQVVTITSSEHVASIFAPLLADLIQEEFWVLFLSRANKVVAKMKISIGGIHQAVVD
                     TRIIFKYALQHAASGIVLCHNHPSGALLPSQEDKNITEKIKQGAKLFDMNVLDHIIIA
                     GDSYYSFADHNIL"
     gene            complement(1753..2004)
                     /gene="rpsT"
                     /locus_tag="PLD76_03100"
     CDS             complement(1753..2004)
                     /gene="rpsT"
                     /locus_tag="PLD76_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOU27024.1"
                     /translation="MANHKSSVKRIRQTEKRKLHNRYYAKTARNAVRKLRALREKEEA
                     VALFPKIVAMLDKLAKKNLIHKNKASNLKSKLAKKVNSL"
     gene            complement(2029..2100)
                     /locus_tag="PLD76_03105"
     tRNA            complement(2029..2100)
                     /locus_tag="PLD76_03105"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2065..2067),aa:Glu,seq:ttc)
     gene            complement(2209..2835)
                     /locus_tag="PLD76_03110"
     CDS             complement(2209..2835)
                     /locus_tag="PLD76_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27025.1"
                     /translation="MNNMDVSKRIAQFFTIFFQPMLIPIYGVLLLIDSGYLYLYTAIG
                     QWLVLGIAIFFSVFVPAVSAVILYKMGIVTSPQIAKREERFYPYLISVISLGMLAYVY
                     TILQVPQFLLYLVYGLLVSVVSVFFVNMWWKISAHMSGIGGLLGGVLTTTYYFKENAM
                     WLYIALFIISGMVAFSRLKLKAHSLGQLFAGFLVGLVCVGGVPLFLFL"
     gene            complement(2839..4728)
                     /locus_tag="PLD76_03115"
     CDS             complement(2839..4728)
                     /locus_tag="PLD76_03115"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HOU27026.1"
                     /translation="MKIHMGDYCLDIYNYHRRNTDQVLIGTVWVGSKYPIRVQSMTNT
                     STMDTDASTAQCEELIDAGAELVRLTTQGVREAQNLYFIRKNLLAKGYVVPLVADVHF
                     NPKVAEEAARIVEKVRINPGNFVDSIKTFQQYEYTDEEYAKEIAKIEEKLIPLLEICK
                     ENSTAIRIGVNHGSLSDRIMSRFGDTPQGMVESCMEFLRICRKHNFLRIVISVKASNT
                     MVMVHTVRLLVATMQQEGMRFPLHLGVTEAGDAEDGRIKSAVGIGALLIDGIGDTIRV
                     SLSEHPKNELPVANKMVDYINQVAKAPTIEPFIYHNNFFKPFAYNRRKSIPVGLIGNT
                     HVPIVIHSLSDTENCIDVTATKPDFIYVGSSIVNTYPTPKIIDAAVWRKEENTYPMFS
                     IETYTNIQMCDADIVFLSMKSTEITKEICELISATTRVILIVQLLSENPVGEGRKVFA
                     TLHYYQVENPVVLQRAYNEEDIEMLQIKSSVDIGSFFLDGFGDGIMLENKQKIQDTVS
                     LSFSILQATRARMSKTEFISCPGCGRTLFDLQQTIAHIKRATSHLNHLKIGIMGCIVN
                     GPGEMADADYGYVGAGKGKISLYKKQECVQRNIPEKEAVQRLIQLIKDNGDWYDNGAE
                     NELMQ"
BASE COUNT         1578 a          970 c          651 g         1565 t
ORIGIN      
        1 ctttaggcac aattatatat tctttatcgg gtctttcttt tttcatttga tgtagatttg
       61 tactagcaaa atatgatgga tgcaaaccaa caatcaacgt acgataaaac aaatccttaa
      121 tgatattaaa tggattgtat aatgaatatc catcatattt acccattgga cctacgtgaa
      181 gaaagaaaat aggacgcaga ttataatcat tgtgcatatc aactatatac tcaacattaa
      241 aataaggatc aggggctcta ttcaaaataa catttaaatg atatctaaaa tcctcatatt
      301 tcccagttat gagtttttga aataaacgaa taatattaat tgaaagaaac ttcgacttat
      361 atttatacac atgatcaata tcaatagaag gacaaaatcg aaaacgagat ttagatttta
      421 tagtatacat gggaaaataa tccgccaaca tctgatctaa caaatatgcc caatgatcta
      481 ccaagggata atctaaaaat tgatgtttat atgccaaact attttctgct ttatatcttc
      541 catgctcatc taagtcaaga ctaccatatt cctcgtatcg cgtcagaaga taaaaagatg
      601 cggcaaaaat gtcaaatggt atagaaacat cggctgattg tgattgaaaa aatatttttg
      661 ttccattcca ttctgtaact tctggttgaa tatctttaat tcccctttta aacaataacc
      721 cttcaggctc aatgacaaag gattttggaa tacgatgttc tctagagtaa caaataacag
      781 gaatagtact atcaatagca acttgctcct ttgtggtaaa agatatatcc acatgcaaaa
      841 tttctttaaa tatttgctca aaaatatatt ttactctagg agtatattta tctgtaaaga
      901 ccaataccat aggctactat ctataaaata ttatggtcag caaaactgta atacgaatct
      961 cccgcaataa taatatggtc tagcacattc atatcaaaca atttagcccc ttgctttatt
     1021 ttttctgtta tgtttttatc ttcttgactt ggtaataacg ctcccgatgg atggttgtgg
     1081 cacaacacga tacctgatgc tgcatgttgt agtgcatact taaaaataat ccttgtgtcc
     1141 actactgcct gatgaattcc tccaatcgaa atcttcattt tagctactac cttgttggct
     1201 ctagataaaa aaagtaccca aaattcttct tgaattaaat ctgcaagaag tggagcaaaa
     1261 atagaggcta catgctcgct tgatgtaata gttacaacct gattagcatc aatccttttt
     1321 cgccgtctcc ccaactctaa ggcagctact atactaattg cttttgcatt tcccacccct
     1381 ttagtagatt ttgttaactc ttttacagat aacctactta attgatttaa atcattattt
     1441 gctaacaaca atattttctt agctaactca acagcagttt cattacgagt gcctgaccca
     1501 ataagtatag caattagctc tgcatcagac aaagcagcta tccctttttc taacaacttc
     1561 tccctaggtc tatcctcttg cgaccattct ttaatactca acaacttcgt ttccatcatt
     1621 cgacaccaaa aatacagaat tattaaagta aaaaaaaata ttcattcaac tatttacatt
     1681 tgtcaataaa aaaaatggct ttcggtaatc tacagaaagc cattttatat atagttaagt
     1741 aagtattact ttttataagc tatttacttt tttagccagt tttgatttca aattacttgc
     1801 tttatttttg tgaataagat tctttttagc taatttatct aacatagcta caatttttgg
     1861 aaacaatgct acagcttctt ctttttcacg tagagcacgt aattttctta cggcattacg
     1921 agctgtctta gcataatatc tattatgcaa ttttcttttt tcagtttgtc tgattctctt
     1981 tacggatgat ttgtgatttg ccatctttca atctattatt ataatttgta gcccctaggg
     2041 gagtcgaacc cctctttcaa gaatgaaaat cttgcgtcct aaccgataga cgaaggggcc
     2101 atcggacaat tcaaaacttg gacgtttttc tgaattgcgg gtgcaaatat acgggagttt
     2161 tttaattctg caaaatttta ttgcgatttt ttgcacaaaa aacaactttt ataaaaaaag
     2221 aaacaacgga acacctccaa cacacaccaa accaaccaaa aacccagcaa ataactgacc
     2281 caaagaatgt gctttaagtt tcaaccgaga aaatgccacc attccggaaa taataaaaag
     2341 agcgatatac aaccacattg cattttcttt aaaataatac gttgtagtaa gaaccccgcc
     2401 aagcaatcct cctattccac tcatgtgtgc actaatcttc caccacatat ttacaaaaaa
     2461 cacagataca acggatacta aaagaccata caccagatac aataaaaatt gaggcacttg
     2521 aagtattgta tatacataag ctaacatacc caatgaaata accgaaatga gatagggata
     2581 aaaacgttct tctcttttgg ctatttgagg agaagttact atacccattt tatataaaat
     2641 aacagcacta acagcaggca caaacacaga aaaaaatata gcaataccca aaacaagcca
     2701 ttgcccaatg gctgtatata aatacaaata accagaatct atgagcaata atactccgta
     2761 aataggaata agcattggtt gaaaaaaaat cgtaaaaaat tgtgctattc gttttgaaac
     2821 atccatatta ttcatttact attgcattaa ctcattttca gcaccattat cataccaatc
     2881 tccattatct ttgattagct gtataagccg ttgtacggct tctttttcag ggatatttct
     2941 ttgtacgcat tcttgttttt tatacaaact tatcttccct ttgcctgctc ctacgtatcc
     3001 ataatcagca tctgccattt cgccaggacc attcactata caacccataa taccaatttt
     3061 tagatgatta agatgcgagg ttgctcgttt tatatgtgct atggtttgtt gcaaatcaaa
     3121 cagggttcta ccacaaccag gacacgaaat aaattcagtt ttactcatcc tagcacgtgt
     3181 ggcttgtaaa atagaaaacg ataatgaaac agtatcttgt atcttttgtt tgttttctaa
     3241 catgatacca tcaccaaatc catcaagaaa aaatgaacca atatcaacac ttgactttat
     3301 ttgcaacatt tcaatatcct cttcattata ggcacgttgc aacactactg gattctcaac
     3361 ctgataataa tgtaacgttg caaaaacttt tcttccttct cctactggat tttcagacag
     3421 caattgaact attaaaatta cccgtgtagt agcagaaatc aactcgcaaa tttcttttgt
     3481 aatttctgtc gatttcatcg aaagaaaaac aatatccgca tcacacattt gtatatttgt
     3541 gtacgtttct atcgaaaaca ttggatacgt attttcttcc tttcgccaca cagccgcatc
     3601 aataattttt ggcgttggat atgtattaac gatactcgat cccacataaa taaaatctgg
     3661 ttttgtagca gtaacatcta tacaattctc tgtatctgat aatgaatgaa tcactattgg
     3721 aacatgcgta tttccaatca gtcctacagg gattgatttt ctgcgattat acgcaaaagg
     3781 cttaaaaaag ttattgtggt aaataaatgg ttctatcgtt ggtgcttttg ctacttggtt
     3841 aatataatct accatcttat ttgccaccgg caattcattc tttggatgct cactaagaga
     3901 caccctaatt gtatcaccaa taccatctat aagtaacgct ccaataccaa cggcagattt
     3961 tatacgacca tcctctgcat ctccagcctc tgttacccca agatgcaatg gaaatctcat
     4021 tccttcttgt tgcattgttg ccaccaataa tcttacagta tgcaccataa ccatggtgtt
     4081 cgatgctttt acagaaataa caatacgtaa aaaattatgt tttctacaaa tacgcaaaaa
     4141 ctccatacac gattccacca tcccttgtgg agtatcacca aaacggctca ttattctatc
     4201 actcaacgat ccatgattca cgccaatacg aatagcagta gaattttctt tacaaatttc
     4261 tagtaatggg atcagttttt cttcaatttt agcaatctct ttggcatact cttcatcagt
     4321 atactcatat tgctgaaaag ttttaatact atccacaaaa ttacctggat taatccgcac
     4381 tttttctact attcttgcag cttcttcagc aactttggga ttaaaatgaa cgtctgccac
     4441 aagtggcaca acatagcctt ttgccaaaag gttcttacgt ataaaatata agttttgagc
     4501 ctctcttacg ccttgcgttg ttaaacgcac caactctgct cctgcatcaa ttaattcctc
     4561 acactgagca gtacttgcat cagtatccat cgttgatgta ttcgtcatag actgtactct
     4621 aatgggatac tttgaaccta cccaaacagt accaatgagc acctgatctg tatttctacg
     4681 atgataatta tatatatcaa gacaataatc tcccatgtgt attttcataa aaattcaacg
     4741 actctttcca ttttcatact tctt
//
LOCUS       DAOSMY010000091         4752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_17982_length_4752_cov_5.007026, whole genome shotgun sequence.
ACCESSION   DAOSMY010000091 DAOSMY010000000
VERSION     DAOSMY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17982_length_4752_cov_5.007026"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2924)
                     /locus_tag="PLD76_03120"
     CDS             complement(<1..2924)
                     /locus_tag="PLD76_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOU27027.1"
                     /translation="MQQISHKQSLFRIFFAIILLFSTLPIFSQGVGQYPVQVSTVVLP
                     NPPMYLSAYSGVETDKLSVTLLNRDTQQSSLQVKLRMRIYCSQGQEISSRAESLYTPI
                     TLEGGIPLRLSMSDLAAYFRTENLRIAGSLPQGKLPVGHTRFCFEVLEYYTGRLLSSQ
                     GCGSAFIVLQTPPLLQQPSHGSLLTDDPTGGILFRWSPQHSVGLERVQYEFILKEMFD
                     TQQSAELGFAHGVEVYRTHTTATSLLYGMMQPPLIAGKRYVWQVRAFTDPISGTPLAF
                     ENNGQSRVFTFDIDYPCQAPQHIIATAHTNGIHLTWDASPHPSASYHLSYRRKDFSST
                     WHQAVTTSNSYTISNLTPGANYEYKVGISCTNKTNDIHYSTVAHIAYNPDDSETPPTS
                     IQTKVKECGKSTPNNISNTEPKETLWAGDVIYAGDFTITLSTVQGSNGRFTGEGKVHV
                     PYLSGIGMSVYFSDISINTDNQLIEGKIHTVYDESRGTIIDLDAFDHGGTTTPAQGVL
                     VPSYEFDFALTNVTRIHYDPLTNSLIFYDATGSRVGNYELTGTAPSFPILVSDKEGNM
                     YSINETNKDNPPANYTLVAVGKQIGELAGNAFDRNVVDSHKGTVTFFDAGVGTYSFDE
                     FLPPYDNINYIRYSSENGNERLYPQLGSNYYVPWKFIPLNTHDKVQAKISLAPNSELQ
                     PERVVFITESGTQVPASYNASNRTYQVSVTGTSNSGVHTLYALYPKGDGTSYFHLGQL
                     RIDSRPPQHLKLVLVYAGGKYDKIAVAHELNTIYNRVGIHWQVSEVQNFTYDKETIKA
                     LFDKKSGLLSAYNDRMTSLNQALQSHLGDSYQSDACYVFMADASQAGNDRDVVGFMPR
                     GRQYGYIYTKDLNASKIGSILAHELAHGKFKLYHTFDNIYAGKLAQGSTANLMDYTGG
                     THLAKWQWNQLYDPAILNGVFDRDEDGEYNIGDLFNWVHETLFGKNTPTVEKKITRNV
                     IS"
     gene            complement(2926..>4752)
                     /locus_tag="PLD76_03125"
     CDS             complement(2926..>4752)
                     /locus_tag="PLD76_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU27028.1"
                     /translation="VSNGTCTSASSGNVVINAVPTAPVLAGATAVCVASTANVTPSTA
                     GTWTSSQPAFATVSNAGVVTGVAAGTAVLTYTRTSDGCSATRNITINALPTAPTVGTI
                     TQPSCTSPTGSVVLSGLPTGNWIITRTPGNITTTGTTTSTTLTGLPAGATYTFTVSNG
                     TCTSASSGNVVINAAPTAPAIPTIASVTQPSCTVSTGAITITSQSGVEYSINNGTSYQ
                     SSPVFIGLLPNAYTLRVRSVGNPTCSTVATNTVSINNVPISTVSVVATAQSIRCHGSA
                     DGRIHFTAQGGIGTLTATLRNAQNQIVDTKTVTQNVATSFTNLSAGTYTLLITDSNGC
                     TAQPAVNEFIIVEPSPIVWQLPTQTITLCKGQQISYDITQLPSLQATYKWYKNNVLVS
                     SLPTYTLSEKGNYRVVITTASLCEQTHSFDVQASSSIIASEFLMASTAKKNEPVKLIN
                     ISNAPIETATWVLPNLPAVSFTNKDMYGIDLLFDAFGTYTIGLKTTVGQCELVLYKNI
                     RIVPDNELNDYPQEKEAFLKKFIVSPNPSNGNFEVTIEFSEVTDVVLSIVDIHTGTLM
                     NKRSLKGNHWYKQAYTPFLPQGQYIVYLQTHKAQSAFKLIIK"
BASE COUNT         1164 a          970 c         1152 g         1466 t
ORIGIN      
        1 aaagatataa catttcttgt aatctttttt tctactgttg gcgtattttt accaaaaaga
       61 gtttcatgta cccaattaaa taaatcacca atattatact ccccatcctc atccctatca
      121 aacaccccat tcagtatggc ggggtcgtag agttggttcc attgccattt ggcaaggtgg
      181 gtgcctccag tgtagtccat taggttggcg gtgctgcctt gggcgagttt gcctgcgtat
      241 atattgtcga aggtatggta cagcttaaac ttgccatggg ctagttcgtg ggctaagatg
      301 ctgcctatct tgctagcgtt taagtctttg gtatagatat acccatactg cctgccacgg
      361 ggcataaagc ctactacatc gcggtcgtta cctgcttggc ttgcgtctgc cataaataca
      421 taacaggcat cggactggta gctatcgccc aaatggctct gcagggcttg gtttaggctg
      481 gtcatacggt cgttgtaggc acttagtaag ccacttttct tgtcgaacag ggctttaatg
      541 gtttctttgt cgtaagtaaa attctgcacc tcgcttacct gccaatggat acctacgcgg
      601 ttgtagatgg tattcagctc atgggctact gctattttgt cgtacttgcc tcctgcatat
      661 accagtacca gcttgaggtg ttgtggtggg cggctgtcga tacgtagctg tcctaaatga
      721 aaatagcttg tgccatcgcc tttcgggtac aaggcataca gcgtatgtac gccactgttg
      781 gatgtgcctg ttacgcttac ttggtaggtc cggttgctcg cattatagct tgctggtact
      841 tgcgtgcccg attcggttat aaacacaact cgctcgggtt gtaattcgct attcggggct
      901 aagcttatct tggcttgtac cttgtcgtgg gtattgagtg gaataaactt ccacggcaca
      961 taatagttgg atccgagttg tgggtacagt ctttcgttac cattttcgga agaatagcgt
     1021 atataattta tattgtcgta cggcggtaaa aactcatcga aactatacgt tcctacacct
     1081 gcatcgaaaa atgtgacggt gcctttgtgg ctatccacta cattgcggtc gaatgcattg
     1141 cctgctaact cgcctatttg tttgcctacc gccactaagg tatagtttgc tggggggtta
     1201 tctttgttcg tttcgttaat gctatacata ttcccctctt tgtcgcttac cagtattggg
     1261 aagcttgggg ctgtaccagt tagttcgtag ttgcctaccc gtgagcctgt ggcatcgtag
     1321 aagattaggc tattggtaag ggggtcgtag tggatacggg taacattggt cagtgcaaaa
     1381 tcgaactcgt agcttggcac taatacgcct tgtgctggcg tggtagtacc gccatggtcg
     1441 aaggcatcga ggtctataat agtacctctg gactcatcgt acacggtatg tatctttcct
     1501 tctatcagtt ggttatcggt attgatgcta atatcgctaa aatacaccga catgcctatg
     1561 cctgataaat aaggtacatg tacttttcct tcgcctgtaa atcgtccatt actgccttgt
     1621 acagtgctta gggtaatggt aaaatcgcct gcataaatca catcgcctgc ccataaggtt
     1681 tctttgggtt cggtattgct gatgttgttt ggcgtgctct tgccacattc ttttaccttg
     1741 gtctgtatgc ttgttggggg tgtttcgcta tcatcggggt tgtaggctat atgggctact
     1801 gtgctgtagt gtatgtcgtt ggttttgttg gtacaactta tccctacttt gtattcgtaa
     1861 ttagctcctg gggtcaggtt gctaatggta taactattgc tggtagtaac tgcctggtgc
     1921 catgtgctgg aaaaatcttt ccgtctgtag ctcaggtggt agctcgctga tgggtgtggg
     1981 ctagcatccc atgtaaggtg aatgccattg gtgtgggctg tagcgatgat gtgttgtggt
     2041 gcttggcatg ggtaatcgat atcgaaggta aacacgcgac tctgtccgtt attctcaaag
     2101 gctaaggggg ttccgctgat tgggtcggta aaggctctta cctgccacac atagcgtttg
     2161 cctgctatca ggggtggttg catcatgcca tacagtaagc tggtagccgt ggtgtgggta
     2221 cggtacactt ctacgccatg ggcaaatcca agttcggcac tctgttgggt atcgaacatt
     2281 tcttttagga taaattcgta ctgtactcgt tccagtccta cgctgtgttg cggactccaa
     2341 cgaaacaata tgcctcctgt ggggtcgtct gtcaataggc tgccatggct tggttgctgc
     2401 agcaatggtg gggtttgcag tacaataaag gcactcccac agccttgact cgacagtaac
     2461 ctgccggtat agtattctaa cacttcaaaa caaaagcggg tatgccctac tgggagtttg
     2521 ccttgtggta agctacctgc tatacgcagg ttttctgttc tgaaataggc tgccaaatcg
     2581 gacatgctca accgcaacgg aatgcctccc tctaaggtaa taggggtata gaggctctcg
     2641 gctcggctac tgatttcttg tccctgagaa cagtaaatac gcatacgcaa cttgacttgc
     2701 aaactgctct gttgcgtatc tctattgagc aaggtaacgg atagtttatc ggtctctact
     2761 ccactgtatg cggataaata catcggcgga ttgggcaata ctactgtact gacttgaaca
     2821 ggatattgtc ctactccctg cgaaaatatg gggagcgtgg agaataaaag gataatagca
     2881 aagaaaatcc taaataatga ctgtttatga ctgatttgtt gcatattatt ttatgataag
     2941 tttgaatgcc gattgagctt tatgtgtttg taaataaaca atatattgac cttgaggcaa
     3001 aaatggcgtg taggcttgtt tataccaatg atttcctttt aaactacgtt tgttcatcag
     3061 tgtgcctgta tgtatatcaa caatggataa aaccacatca gtaacttctg aaaattcgat
     3121 agtaacctca aaattgccat tagatggatt gggagacaca atgaattttt tgagaaaagc
     3181 ttctttttct tgtgggtaat cattaagctc attgtcagga acaatacgaa tatttttgta
     3241 taaaacaagt tcacactgcc ccacggtggt tttcagaccg atagtatatg taccaaaagc
     3301 atcaaacagc aaatcaatac cgtacatatc tttgttggta aaactgacag cgggaagatt
     3361 gggcaatacc catgtagctg tttcgatggg agcattagaa atattgatta acttcacagg
     3421 ttcgtttttc ttggcagtac ttgccatcaa aaattcagat gcaattatac tgctacttgc
     3481 ttgtacatca aacgaatgtg tttgttcgca taaactggct gtagttatca caacacgata
     3541 attccctttc tcgctaaggg tataagtagg caatgaagat accaacacat tgtttttgta
     3601 ccatttgtaa gtagcctgca aactaggtaa ttgggtaata tcataactaa tttgttgccc
     3661 tttgcataaa gttattgttt gggtaggtaa ttgccaaaca ataggacttg gttcaacaat
     3721 aataaactca ttgactgctg gttgagctgt acagccattg ctatcggtaa taagcaatgt
     3781 ataggtgcca gcagataaat tggtaaatga ggtagccaca ttttgggtta ctgtttttgt
     3841 atcaactatt tgattttgag cattacgtaa ggttgccgtt aaggtaccaa tacccccttg
     3901 tgctgtaaaa tggatacgac catctgcact gccatgacag cgaatagatt gggcggtagc
     3961 cacaacactt actgtagaaa taggaacgtt attgatggaa acagtatttg tagctacagt
     4021 tgaacaggta ggattaccaa cactacgtac ccgtaaggta taagcatttg gtaataagcc
     4081 aataaataca ggacttgatt gatagcttgt cccgttgttg atactgtatt ctacccctga
     4141 ttgtgaagta atagtaatgg ctcccgttga taccgtgcaa cttggttgtg taacacttgc
     4201 tattgtagga attgctgggg cagtaggtgc agcattgata actacattgc cactagaggc
     4261 tgaagtacag gtaccattgc ttacggtaaa ggtatacgtt gctcctgcgg gtaaacctgt
     4321 gagggtggta ctggtagtag tacctgtggt cgtgatattg cccggcgttc tggtaattat
     4381 ccagttgcca gtaggtaaac cgctcaacac cacagagcct gtagggctag tacaacttgg
     4441 ctgggtaata gtgcctaccg ttggggctgt gggaagggcg ttgatggtga tgtttctggt
     4501 ggcggaacat ccatcgctgg ttctggtata agttaataca gcggttcctg ctgctactcc
     4561 tgtaacgact cctgcattgc ttacggtagc aaaagcgggt tggctacttg tccatgtacc
     4621 tgctgtagaa ggcgttacat tggctgtgct ggctacacag acggctgttg ctcctgctaa
     4681 tacgggggca gtaggcacag cattgataac tacattgcca ctagaggctg aagtacaggt
     4741 accattgctt ac
//
LOCUS       DAOSMY010000092         4657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_18467_length_4657_cov_3.534333, whole genome shotgun sequence.
ACCESSION   DAOSMY010000092 DAOSMY010000000
VERSION     DAOSMY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18467_length_4657_cov_3.534333"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PLD76_03130"
     CDS             <1..1141
                     /locus_tag="PLD76_03130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU27029.1"
                     /translation="IGFDVTVSAINHNFPYYSEKDYPYAIYNGKLFGDDLAVRMTSAL
                     LESAKFDVLFIMNDVNVILECLKPILMAKKRNPDMKVIFYPAMDVDYVTEADGVVSEV
                     ADYMIWWTDFAYNNVVKEYPKYKDKMSYIPFGFDTDVFYREGNKKTRKYRKEIWGIED
                     DVYVVGQVNRNQWRKDYFKLIHAFALFNKKHPKSLLYIHAKQNDIGGNLPALIFATAT
                     WLKINPREFSQKVMMPNDEEFNTTIGFPIKQLRLIYNSMDLHVSTNQGEGWGLTTAEA
                     MCCETPVIVPDNTSGKMFCGDDRGLLVKCGESLNDWRVNYGNTGGSLLPRPSVDVLDL
                     LDKMEFAYKNKEKLREMTKRGRIYTENNNWKAVENKWIGFLKKII"
     gene            1152..2318
                     /locus_tag="PLD76_03135"
     CDS             1152..2318
                     /locus_tag="PLD76_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU27030.1"
                     /translation="MEWKTVKKRLSELKPLENNPRKISNENLERLGRDMENLGNFRPL
                     VCDVDGTVLAGNQRYKKLLEKYGQAHEVEVSVPEKPLTEKQRKEVVILDNKHRGEDDI
                     DMLMKDYSDTLESLGFDDLIPEPEIEVKEDNYEEPEDLQTTVVLGDVYQLGDHRLMCG
                     DSTKVDDVEKLMNGEKADMVFTDPPYGMNLDTDYSSMKSEIFKGGIGGNKYDAGIVDN
                     FDPEMINTIMLLKARETFIWGADYFAELLPNKNDGSWVVWDKRANGNDDIEQDKSSDK
                     MYGSCFELCWSKNKHKRDIARVKWAGIFGMSSQDQNKRIHPTQKPLELCCWFLKRYSK
                     TRENIVDLFGGSGSTLIACEQTNRKCYMMELDPYYCSVIINRWETYTGKGAVKI"
     gene            2525..2893
                     /locus_tag="PLD76_03140"
     CDS             2525..2893
                     /locus_tag="PLD76_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOU27031.1"
                     /translation="MDYRLRFKGEKSHLWEGGKTSRNKKMRTNPAFKAWRTLVFERDN
                     YTCQCCNARCRKGKRVELHPHHILELSKYPEYAYDVENGITLCSDCHWAYHRGTLDPH
                     YCQVIIDRWEKFTNKKAKKL"
     gene            3106..4371
                     /locus_tag="PLD76_03145"
     CDS             3106..4371
                     /locus_tag="PLD76_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOU27032.1"
                     /translation="MKPLENNPRKISNENLERLGRDMENLGNFRPLVCDTDLTVLAGN
                     QRYKKLLEKYGGGYEVEVSIPEKQLTEKQKKDVVILDNKHRGEDDIDVLMSEYKDTLE
                     GLGFDDLIPEPVLEVKEDNYEEPEILPSKVKRGEVWQLGRHRLMCGDSTKVDDVERLM
                     NGQKVDMVFTDPPYNGDVANNWDNQWKNWEEFGQFIRGTVFNIDCNLYCVWGNWKSSN
                     AIYGQAIKSNLNFLQEIIWLKPASIGMLNWFPKVTEYMSIFYKKKDLVVNNVENTRID
                     LEEDFLPKSDLRISMYEKARRGDFDNSSDKRRRADKFVAEHGTKPKNVWYFKNMNGGY
                     GFGSNKNSNEYSEHTTIKPVKLCERGIIAFSRENHLILDLFGGSGSTLIACEQTNRIC
                     YMMELDEHYCDVIIDRWEKLTGKKATLIK"
     gene            4380..>4657
                     /locus_tag="PLD76_03150"
     CDS             4380..>4657
                     /locus_tag="PLD76_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27033.1"
                     /translation="MSNKTAPKWQPTKDMWNVLKAWCDMETKPTVKDRMERAGVRREN
                     WWEWIRKPEFVRWWNKQTEDFMKTTISDLNRISYLKASSDFRYMELLQ"
BASE COUNT         1684 a          546 c         1160 g         1267 t
ORIGIN      
        1 tattgggttt gacgtaacgg tgagtgctat taatcacaac tttccgtatt attctgagaa
       61 ggattatccg tatgcgattt ataacggtaa attgtttggc gatgacttgg ctgtaagaat
      121 gacgtcggca ttgcttgaga gtgcgaaatt tgacgtgctg tttatcatga acgacgtaaa
      181 tgtcatactt gaatgtttaa aaccgatttt gatggcaaag aagcgcaatc ctgacatgaa
      241 agtaatattc tatccagcga tggacgttga ttatgtaact gaagctgacg gagtagtaag
      301 tgaagttgca gattatatga tatggtggac agattttgcg tataacaatg tggtaaaaga
      361 atatccgaaa tataaggata aaatgagtta tataccattt ggatttgata cagatgtgtt
      421 ttatagagaa ggcaataaaa aaacaaggaa atatcgaaaa gagatttggg gtattgaaga
      481 tgacgtttat gtagtggggc aggttaatcg caatcaatgg agaaaggatt atttcaaatt
      541 aattcatgcg tttgcgttat ttaataagaa gcaccctaag agtttgttgt atattcatgc
      601 caagcagaac gatataggag gtaatttacc agcgttgata tttgcaacag cgacttggtt
      661 aaagatcaat cctagagagt tttcacagaa agtaatgatg ccgaacgatg aagagtttaa
      721 tacaacgata ggatttccga ttaagcaatt gaggttaata tataattcaa tggatttgca
      781 cgtaagcaca aatcaaggtg agggttgggg attaactaca gctgaggcga tgtgttgtga
      841 aactcctgta atagttcctg ataatacaag tggcaagatg ttttgtggtg atgatagagg
      901 actattggtt aaatgtgggg agagtcttaa cgattggagg gttaattatg gaaacacggg
      961 aggaagttta ttgccgagac cgtcagttga tgttttggat ttattggaca aaatggaatt
     1021 tgcgtataag aataaagaga agttgcgaga aatgactaaa cgtggtagga tatatacaga
     1081 gaataacaat tggaaggcag ttgaaaataa gtggatagga tttttaaaaa aaataattta
     1141 aattagcggt tatggaatgg aaaactgtta aaaaaagatt gtcagaatta aagccattag
     1201 agaacaaccc acgtaagatt agtaatgaga acctggagcg attaggacgt gatatggaga
     1261 atttagggaa tttcagaccg ttggtatgcg acgttgacgg tactgtatta gcaggaaatc
     1321 agagatataa gaaactttta gagaaatacg gtcaagcaca tgaagtagag gttagcgtac
     1381 ccgaaaagcc attgacagaa aaacagagaa aagaagttgt aattttagac aataaacaca
     1441 ggggtgaaga tgacattgat atgctgatga aggattacag cgacacgttg gaaagtttag
     1501 ggtttgatga tttaatacca gaaccagaga ttgaagttaa ggaggacaat tatgaggagc
     1561 ctgaggattt gcaaactact gttgtattag gagatgttta tcaattaggg gaccacaggt
     1621 taatgtgtgg ggatagtaca aaagttgatg atgttgagaa gttgatgaat ggagaaaagg
     1681 cagacatggt gtttacagac ccaccttatg gaatgaattt agatacagat tatagcagta
     1741 tgaagagtga aatttttaaa ggtggaatag gtggtaataa atatgacgct ggtattgtag
     1801 ataattttga cccagaaatg ataaatacaa ttatgttact aaaagcgaga gaaacattta
     1861 tatggggtgc tgattatttt gccgagttat tgcctaataa aaatgatggt tcgtgggtag
     1921 tgtgggataa gagagcaaac ggaaatgatg atatagagca ggataaatca agtgacaaaa
     1981 tgtatggtag ttgttttgaa ttgtgctgga gcaaaaataa acacaaacga gatatagcaa
     2041 gagtgaaatg ggctggaata tttggaatga gtagtcaaga ccaaaataaa aggattcatc
     2101 caacgcaaaa accattagaa ctgtgctgtt ggtttttaaa acgatatagc aagacgcgtg
     2161 aaaacattgt tgacctattt ggtggaagtg gttcaacact aatagcatgt gaacaaacaa
     2221 accgtaaatg ttatatgatg gaattggacc catattactg ttccgtaatt atcaaccgat
     2281 gggaaactta tactggcaag ggggcagtaa agatatgagc agaaagaatg gtaagaactt
     2341 aatatgtgaa gtgtgtggta aaacctatta cagaagtggt aggaggtgta atacatctag
     2401 atattgcagt aaagaatgtt ggggtaagag aagccctaaa atagaagtta gctgtttgtt
     2461 ttgtggaaag gtgtttatgg agtacgaggg gagaaaggtt tattgtagta tggaatgtag
     2521 agatatggat tacaggttaa gatttaaggg tgagaaatct catttgtggg aaggtgggaa
     2581 gacaagcaga aacaaaaaga tgaggactaa ccctgcattc aaagcttgga gaacattagt
     2641 atttgagaga gataactata cctgtcaatg ttgtaatgcg agatgtagga aagggaaaag
     2701 agtggaatta catccacatc acatactaga attatctaaa tacccagaat atgcttacga
     2761 tgtagagaat ggtataacat tatgtagtga ttgtcattgg gcatatcaca gaggaacatt
     2821 agaccctcat tattgtcagg taattataga cagatgggag aaatttacaa acaaaaaagc
     2881 aaagaaatta tgagcagaaa ttgtggtgca aagtggaaag tttaatttac atgtacttat
     2941 ggacatacaa acagtcaatg tacaggatct gaaatcgtgg gacagaaatc ctcgtgaggc
     3001 tattgatatt gaaagattga aagaagtatt acaaaagagg ggtcaataac ataatttaat
     3061 taaactatta tggaatggaa aacggttaaa aaaagattgt cagaattgaa accactcgag
     3121 aacaatccac gaaagattag taacgagaat ttagagagat tggggcgtga catggaaaat
     3181 ttgggaaatt ttagaccgtt ggtttgtgat actgatttaa cagtattggc tggtaatcag
     3241 agatataaaa agttactaga aaaatatggt ggtggttatg aagtagaggt tagtattcca
     3301 gaaaagcaat tgacagagaa gcagaagaag gatgttgtta tattagacaa caaacacaga
     3361 ggtgaagatg acattgatgt tttaatgagc gaatataagg atacattgga ggggttaggg
     3421 tttgatgatt taattccaga accagtattg gaagttaagg aagataacta tgaggaacca
     3481 gagattttac cgagtaaggt taaaagaggt gaggtttggc aacttggtag acaccgcctt
     3541 atgtgtggtg atagcacaaa agttgatgat gtggagagat taatgaacgg acagaaagtg
     3601 gatatggtgt tcacagaccc tccatataat ggggatgtcg ccaataattg ggacaatcaa
     3661 tggaagaatt gggaagagtt tggtcagttc attcgtggca ctgttttcaa tatagattgc
     3721 aatttatatt gtgtttgggg aaattggaag tcaagcaacg ctatttatgg tcaggcaata
     3781 aaaagcaacc tgaacttttt gcaagagata atttggttaa aacctgcgag tattggaatg
     3841 ctcaattggt ttccgaaggt tacggaatat atgtcaattt tttacaaaaa aaaagattta
     3901 gtagtaaata atgtagaaaa tacaagaatt gatttagagg aagatttttt gcctaaaagt
     3961 gatttgcgta tatcaatgta cgagaaggct cggaggggtg attttgacaa ttccagcgat
     4021 aaacgccgtc gggctgataa gtttgttgcc gaacacggaa cgaagcctaa aaatgtttgg
     4081 tattttaaga atatgaatgg gggatatgga tttggaagta acaaaaatag caacgaatat
     4141 tcggagcata cgacgattaa gccagtaaaa ctttgcgaaa gagggataat agcattctca
     4201 agagaaaacc acctaatcct tgacttattc gggggttcgg gctcgactct tatagcttgt
     4261 gagcaaacta atagaatatg ctatatgatg gaattagatg aacactattg cgatgtaatc
     4321 attgacaggt gggagaagtt aacaggaaag aaagctacct taattaaata actctacaaa
     4381 tgagcaacaa aaccgcacca aaatggcaac ctaccaaaga tatgtggaat gttctaaaag
     4441 cttggtgtga tatggaaaca aagcctaccg tcaaagatag aatggaaagg gcaggagttc
     4501 gcagggaaaa ttggtgggaa tggataagga agcccgagtt tgtaagatgg tggaataaac
     4561 aaacagaaga ctttatgaag accactattt cggacttgaa taggatatct tacctaaaag
     4621 cgagcagtga ctttagatat atggaattat tgcaaat
//
LOCUS       DAOSMY010000093         4644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_18554_length_4644_cov_4.134234, whole genome shotgun sequence.
ACCESSION   DAOSMY010000093 DAOSMY010000000
VERSION     DAOSMY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18554_length_4644_cov_4.134234"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..2174
                     /locus_tag="PLD76_03155"
     CDS             75..2174
                     /locus_tag="PLD76_03155"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU27034.1"
                     /translation="MGANEGLSKKAKIFNEIIKDLQYILAIGIIVVLLPSDLQYKYQY
                     TEGKPWQYDLLTATFDFPIYKTEVEISNQRNDIIKTHKPYYVLDTSLKSKQIAFLELI
                     VQSLFAKDTISPISTHVISELKKSLINLYDTGILTSQDQSALLAKEAKYIVIMQNNLG
                     QTRRVEDIITEKEAIRLFLKDASRILPESTLNKLPINELIIGNLVYDAETTKKIKNDE
                     LNNLSLTSGQIQAGERIIDRGEIVTYEKQKILDSLKKQFHQEERKSNNNTILLGEILI
                     VSCLILLFYLYARLFRKKFFISKRFVSVTLLFIIGFTIATSMIVQHANPKWVYIIPYS
                     LLPIVLSTFFDTRTGLFAHVITVLLSSFMVPLPFEFVLLQITTGMVAIYSLKDLAQRS
                     QLVQSAIIIVISYCLIYFGYGITIDGDITRINWTMFIYFFVNGVLLLFAYPLIYILEK
                     MFGYISNVTLIELANTNNKVLRNLSELAPGTFQHSMQVSNLASEIAIKVGANPLLART
                     GAMYHDIGKMKNPAFFTENQNKGNNPHQQLSFEDSARIIIQHVEDGVQIAKDANIPNC
                     IIEFIQTHHGTNKVRFFYNSYRLQFPEKEIDDSIFTYPGPNPSTKETAIVMLCDAVEA
                     ASRSLPEYTDEAIDGLVEKIITSLLQEGSLNEAPLTMQHISLTKLILKEKLKNIYHTR
                     ISYPELQKNKLKPNETT"
     gene            2161..4242
                     /locus_tag="PLD76_03160"
     CDS             2161..4242
                     /locus_tag="PLD76_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU27035.1"
                     /translation="MKPLRLFTISLLLLFTGYFVNSQNIGIQKNNPDFLEYLSSEEIQ
                     TGYVPLPFEMPTTPVGTKRSGESFPSQFDSRNVNGTSYILAPRNQVITGPCWAFAATD
                     VVQASFYKKGYQEGYLSPQLLTNCHNFVYTKTQGGNSLMAVATFARLVGPAFENAVPY
                     DANETACITTTKEDFPAFVSNVHMINNNIDAVKKAIMDNGSVATKMFYDVAYYNSADK
                     TYFYNGAANENHGISIVGWNDNMVVADKGYGTPSASGVWIVKNTWGTGFGDGGYFYVS
                     YEDTYVGKEVFAFTKRIDKNTIDKVYQYAQHGMTNNVGYNANNPSYGRIKFTTTEREK
                     IAAVGLYTYHPNTEITFWIYEGADFSKLRYTSEAEVIQYGGYYTIDITAPIYITGDFQ
                     VIVSYKHSTENYAIPIEQNFSNYNNATIQNSGVQWISSSGSSWTPLGADTSFKFNLCI
                     NAYTSIPNPPANDNVQDAVLLNTNTVYENFTNAGATVQANEVVPPYNNEEASCYTQNS
                     WCYEGGLQNSVWFKFVATNSVVNIITEGFDNQIALWQSNDDNAENLISGNTSTYQLLA
                     ANDDASVSNAFASLYNVQGLQVGKTYFIQVDGSANGAEGIFSIEIQDIPTSINSTHAS
                     TLLTPEGELTQEIKDKVNYIEVFDVSGRLLQILQKNSSLSNSKLPKQQVLIIKLHYTD
                     GGSDTISWLQN"
     gene            complement(4292..4597)
                     /locus_tag="PLD76_03165"
     CDS             complement(4292..4597)
                     /locus_tag="PLD76_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOU27036.1"
                     /translation="MLRHIVMWKVKEEALGKTKLELCAEICRLLQTLPPHITEINQLS
                     TAINHPLCPADNYDVVLDTVFNSVEDLQTYQVHPKHVEVASFIKQVVFQRACIDVEC"
BASE COUNT         1620 a          769 c          823 g         1432 t
ORIGIN      
        1 attgaattct caattaaaat atttacattt gcttgttaat tttgtagtat tttctctgaa
       61 aagaagaaag cgttatggga gcaaatgaag gtctgagtaa aaaggcaaaa atatttaatg
      121 aaattattaa ggatctccag tatattttgg cgataggtat tattgtagta ttacttccca
      181 gcgatttgca atacaaatat caatatactg aaggcaagcc ttggcagtat gatttactta
      241 cggctacatt tgatttccca atttataaaa cagaagttga aatcagtaat cagcgtaatg
      301 atataataaa aacgcacaaa ccttattacg tactagatac aagccttaaa agcaagcaaa
      361 ttgcattctt agaattaata gtacaatcgt tattcgcaaa agacacaatt tcgccaattt
      421 ctacccatgt aatttctgaa ctaaaaaaat cgttaataaa cttatatgat actggaattt
      481 taacgtcaca agatcagtct gcattacttg ctaaagaagc taaatacatt gtgattatgc
      541 aaaataatct aggtcaaaca cggagagtag aagacattat cacagaaaaa gaggctatca
      601 gattattttt aaaagatgca agtcgtatac ttccagagtc tacacttaat aaactaccta
      661 ttaatgaatt aataataggc aatttggtgt atgatgctga aacgacaaaa aaaataaaaa
      721 atgatgaatt gaataattta tcgctcactt cgggacaaat acaagctgga gaacgtatta
      781 ttgatagggg agaaattgta acttatgaga aacaaaagat cttagactcc ttgaaaaaac
      841 aatttcacca agaagagaga aaatcgaata ataatacaat tcttttagga gagattttga
      901 ttgtaagctg tttaatttta ttgttttacc tttatgctcg tttatttagg aagaagtttt
      961 ttatcagcaa acgttttgtt agtgttacgt tattattcat aattgggttc actatagcaa
     1021 cttcgatgat tgtgcaacat gctaatccta aatgggtata tattatccct tattctttgc
     1081 tacctatcgt gttaagcacc ttttttgata cccgtacagg attatttgca catgttataa
     1141 ctgtattgct ctcttcattt atggttccat tgccatttga atttgtttta ttacaaataa
     1201 cgacaggaat ggttgcaatc tacagcctaa aagatttggc tcaacgctcc cagctagtac
     1261 aatcagctat cattattgtt attagctact gcttaattta ttttggatat ggcattacaa
     1321 ttgatggaga tattactcgc attaattgga ctatgtttat ttatttcttt gtaaatggag
     1381 tccttttatt atttgcttat cctctcattt atattttaga aaaaatgttt ggatatattt
     1441 ctaacgtaac attaattgaa ttagccaata caaataataa ggtactccgt aatttatcgg
     1501 aactagcccc tggtactttc cagcattcga tgcaagtgtc gaatttggct tcagagattg
     1561 caattaaggt aggagctaat ccattacttg ctcgcacagg agccatgtat cacgacattg
     1621 ggaaaatgaa aaatccggct ttctttacag aaaatcagaa caaaggaaat aatcctcatc
     1681 aacagttgag ttttgaagat agtgctcgta ttattatcca acatgttgaa gatggtgtgc
     1741 aaatagccaa agatgcaaac atccccaatt gtataattga atttatccaa acacatcacg
     1801 gcacaaacaa agttcgattt ttttacaatt cgtaccgttt gcaattccct gagaaagaaa
     1861 ttgacgatag tatatttaca taccctggac caaacccaag tacgaaagaa acagctattg
     1921 ttatgttatg tgatgcagta gaggctgctt cacgtagttt gcctgaatat acagacgaag
     1981 ctattgatgg tttagtagaa aaaattatca catcattatt acaagaaggc tcgttaaatg
     2041 aagcaccctt aacaatgcaa catatatcat tgacaaaact catattaaaa gagaaactaa
     2101 agaacattta ccacactaga atatcttatc ctgaattaca aaaaaataaa ttaaaaccca
     2161 atgaaaccac ttagactgtt tactataagt ttgttattac tgtttactgg ctattttgta
     2221 aattcacaaa atattggtat tcaaaaaaat aaccctgatt ttttggaata tctttcatca
     2281 gaagagatac aaacgggtta tgttccttta cccttcgaaa tgcccacaac acctgttgga
     2341 acaaagagga gcggagaaag tttcccttca caattcgact ctcgcaatgt gaatggaact
     2401 agttatattt tagctccaag aaatcaggta ataactggtc cttgttgggc ttttgcagct
     2461 acagatgtgg tacaagcttc tttttataaa aaaggatatc aagaaggata tttatcacca
     2521 caactattaa caaactgtca taacttcgtg tatacaaaaa cacaaggagg aaattcgttg
     2581 atggcagtag ctacatttgc ccgtttagtt ggtcctgctt ttgagaatgc ggtaccatat
     2641 gatgcgaatg aaacagcctg tataacaaca acaaaagaag actttccagc atttgtttcg
     2701 aacgtacata tgataaataa taatattgat gcggtaaaaa aagcaatcat ggacaatgga
     2761 tcggttgcaa caaaaatgtt ttacgatgta gcttattata atagtgctga taaaacgtat
     2821 ttttataatg gagcagccaa cgaaaatcat ggtattagca ttgtgggttg gaacgataat
     2881 atggtggttg ccgacaaagg atatggaact ccatcagctt cgggtgtttg gatagtaaaa
     2941 aacacttggg gtactggttt tggagatggg ggttattttt atgtatcgta cgaagataca
     3001 tatgttggga aagaggtatt tgcatttacc aagcgtattg ataaaaatac cattgacaag
     3061 gtatatcaat atgcacagca tgggatgaca aataatgtag gctataatgc caataaccca
     3121 tcatacggac gcattaaatt tactaccact gaacgagaga aaatagcagc cgtaggtttg
     3181 tatacatacc accccaatac cgagattaca ttttggattt acgaaggagc tgattttagc
     3241 aaattacgct atacatccga agcagaagta attcaatatg gaggatatta tacaatagat
     3301 attactgcac caatatatat aactggagat tttcaggtaa tagtaagcta taaacactca
     3361 acggaaaatt atgcaatacc catagaacaa aatttttcta actacaataa tgctacaata
     3421 caaaactcgg gagtacagtg gatcagctct tctggcagtt cttggactcc tctaggtgca
     3481 gatacatctt ttaaatttaa tctgtgtatt aatgcatata cctctatacc aaatcctcct
     3541 gctaacgata atgtgcaaga tgctgtattg ctaaacacaa atacagtgta cgaaaacttc
     3601 acaaacgctg gagctacagt acaagctaat gaagttgtgc caccatacaa taacgaagaa
     3661 gcaagttgtt atacacaaaa cagttggtgt tacgaagggg ggttgcaaaa ttcggtatgg
     3721 tttaaatttg tagcaactaa ctctgtagta aatataatta cagaaggctt tgataatcag
     3781 atagcccttt ggcaatcaaa tgatgataat gcggaaaacc ttatcagtgg caacacatct
     3841 acctatcagc tactagcagc caacgatgat gccagtgtaa gcaatgcctt tgcttcattg
     3901 tataatgtgc aaggattgca agtagggaaa acttatttta tccaagtaga tggttctgcc
     3961 aatggtgcag agggtatttt ctcaattgaa atacaagaca ttccaacttc tattaatagc
     4021 acccatgcat ctactttatt aacacctgaa ggcgagttaa cacaagaaat aaaagataag
     4081 gtgaactata ttgaggtgtt tgatgtaagt ggacgattac tacagatatt gcaaaaaaat
     4141 agtagtttga gcaacagcaa attacctaaa cagcaggtac taatcatcaa gttgcattat
     4201 actgatggtg gtagcgatac cattagttgg ttgcaaaact aacgaattga cttaaaaaaa
     4261 tcctttgatg caagtcaaag gattttttgt tttagcattc gacatcaata catgcccttt
     4321 gaaaaactac ttgtttgata aaactagcca cttctacgtg cttgggatgc acttgatagg
     4381 tttgcaagtc ctctacagaa ttaaatactg tatctaatac tacatcgtaa ttgtccgctg
     4441 ggcataaagg atgattgatg gcagtagata attgattgat ttcggtaatg tgtggaggca
     4501 aggtttgtaa taagcggcaa atttcggcac ataactccag cttggttttg cctaaagctt
     4561 cttctttcac cttccacata acaatgtgtc gcaacataag ggtattattt aaaaattatt
     4621 tcggcacaaa tatacactat tcag
//
LOCUS       DAOSMY010000094         4641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_18568_length_4641_cov_3.458788, whole genome shotgun sequence.
ACCESSION   DAOSMY010000094 DAOSMY010000000
VERSION     DAOSMY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18568_length_4641_cov_3.458788"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLD76_03170"
     CDS             complement(<1..399)
                     /locus_tag="PLD76_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOU27037.1"
                     /translation="MLNSLSHFDLQQYWWLIMSLVGSILVFLLFVQGGQSLISTLGKT
                     ADERNMVVNSIGRKWEFTFTTLILFAAGMFAAFPLFYSTSFGGAYYAWKLFLIVFVIQ
                     AVSFEFRRKKGNLLGTKTYDTFLAINGYVAP"
     gene            complement(392..1948)
                     /locus_tag="PLD76_03175"
     CDS             complement(392..1948)
                     /locus_tag="PLD76_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOU27038.1"
                     /translation="MIENIDLTLVNWSRAQFAMTAFFHWLFIPLTIGMTYIIAFAQTL
                     HVRTNDPEWEKLAKFWAKLFGINFAIGVGTGIILGLEFGTNWSNYSWISGDIFGAPLA
                     AEGIMAFFLESTFVAVMFFGWGRISKKFHLVACYLVAFGASLSALWILTANAWMNYPV
                     GMSFNPDIARHEMVDFWAILFSPFAVNKFLHTISSGFVIGSFFIIAVSSWYLLKGRSV
                     LFAKRSIFLASVFGLVGTGLVILTGDGSAYNVAQHQPAKLAAMEGLYDGKEGAGLVTV
                     GILTPGKKIGDNKDPFIFKQEIPYVLSFLGYRDINAFVPGINDLVYGYKSDKLEIVSA
                     SKKMEYGRVAMQALSLYKKTDNSEEKAMALQLFTEYQPFMGYAHITSPEKVIPNVPMI
                     FYSFHIMVMAGFFLIAVVAGLLFFSIKNKVENHKWLLRLGILGFPMAFIASMTGWIVA
                     EVGRQPWTIQDLLTTSVSTSNINSMAVILTFWMFFALLSVLVIAEVRIMLAAIKKGPE
                     STNTKGGENA"
     gene            complement(1941..2186)
                     /locus_tag="PLD76_03180"
     CDS             complement(1941..2186)
                     /locus_tag="PLD76_03180"
                     /inference="COORDINATES: protein motif:HMM:NF026249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOU27039.1"
                     /translation="MIQQPSSSHFSFIKDGILYIKRSKMAKILLIIILIKFLIFYGFL
                     KGFLYPRYLKPKWESEQHRIESVTHDLIQPLNTISYD"
     gene            2397..4586
                     /locus_tag="PLD76_03185"
     CDS             2397..4586
                     /locus_tag="PLD76_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27040.1"
                     /translation="MTDKEIVLTKENLFYYLSKSQLGRAIELIDNLLTQVQDGYYGDE
                     VYELKTNYKRMLQYMFYGVNDPEQHLVYQHLLKETYEIGEHLADKLLQLNSTQYTYRT
                     KRLFAVQPVISDELVLSGFQRYASLGNRKTESDIREYEMLISYSFDKYWLTSLYSETM
                     LQQVKLLAEDETIGQIELCSLASAITLSSMAFFDEKKCLALVELTKSRYPEVVLRALT
                     GVVLFLYFHHQRISFYPVIRTQITLLFDKEEFIVASQLIIKQFIRSKETEKITKDITE
                     NILPELSKIAPHVQNDLADIDKQEELEDKKNQWQDMIEETGIGEKMQQYAELQSQGAD
                     IQMSTFSMLKNYPFFNVVSNWFLPFYAEHSSVSNLFKEEKENSFLSFLAKSPILCHSD
                     KYSFCLNLTMVPEQYRKAMMNGVQMESEQLKEMTNDKLSLSNTVVAEEIMNKYIQDLY
                     RFYKLFPKNNKNSFDDIFTYKFDFHKTWMFRLLNPTNEFLFEIANFYFAKEMYENALS
                     ALLELEEHTEPSADLYRKIGFSYQKTYNYSLAIEYYLKAELIETENIWTIRKIAYCYR
                     NLKQTEKALEYYLLADSLQPNTIRLLFTIGVCYVEMQDYDTALRYFYKIEFLESNHKK
                     SENYIALCSFLQKKYSQATKYYHKQPDKNTFYELLYIGHAYWCLGNKKEALHYYLQSA
                     YTVKKETKQFKNFYEALEQDTDILLKHGFSLKDIVFIGDAVRMKIES"
BASE COUNT         1617 a          798 c          800 g         1426 t
ORIGIN      
        1 cggcgctaca tacccattga tggctaaaaa tgtatcgtat gttttagttc ctagcagatt
       61 gccttttttg cgtctaaact caaacgatac ggcttgtatc acaaatacaa tcaaaaaaag
      121 tttccaagca taataagctc caccaaacga agtggaatag aacagtggaa atgccgcaaa
      181 catacctgct gcaaaaagaa tcaaggtagt aaacgtaaat tcccatttac gtccaatgga
      241 gttgaccacc atattgcgtt cgtcggctgt tttccctaag gtactaatca atgattgacc
      301 tccttgtaca aaaagaagaa aaacgagtat cgaacctaca agcgacatta taagccacca
      361 atattgttgt aaatcaaagt gtgataatga attaagcatt ttcccctcct ttcgtattag
      421 ttgattcagg accttttttg atagcagcta acatgatgcg aacttcggct attacaagca
      481 cagaaagcag tgcaaaaaac atccaaaacg taagaattac agccattgaa ttgatgttag
      541 atgtagaaac agatgtggtc agcaaatctt gtattgtcca tggttggcga cccacttcgg
      601 caactatcca tcctgtcata gaggcaataa acgccatcgg gaaaccaagg atacccaatc
      661 gcaacaacca tttgtggttt tctactttat ttttgatgct gaaaaacaac aatccagcta
      721 caacagcaat caagaaaaag cccgccatca ccataatatg gaaactataa aaaatcattg
      781 gtacatttgg aataactttt tcaggacttg taatatgagc ataccccata aaaggttggt
      841 attcggtaaa taattgcaaa gccatagctt tctcttcgct attatccgtt tttttataca
      901 acgagagggc ttgcattgct acccttccat attccatttt cttagaagca gatactattt
      961 ctaatttgtc tgatttatat ccatatacca aatcattgat gccaggcaca aatgcattga
     1021 tatctctata gcccaaaaat gaaaggacat aaggtatttc ttgtttgaaa ataaacgggt
     1081 ctttgttatc tccaattttt ttaccaggtg taagtattcc tacagttacc aatcctgctc
     1141 cttctttgcc atcatacaat ccttccatgg cggcaagttt tgcgggttga tgttgtgcta
     1201 cattatatgc tgaaccatcg cctgttaaga taaccaagcc tgtgcccact aatccaaata
     1261 cgctggctag aaaaatacta cgttttgcaa acagtacgct gcgtcctttt agcaaatacc
     1321 aactacttac agcaataata aagaaagagc caattacaaa acccgaagag atggtgtgta
     1381 aaaatttatt tacagcaaaa ggtgaaaata aaattgccca aaaatctacc atttcgtggc
     1441 gagcgatatc tgggttaaag ctcatgccca ctgggtaatt catccatgcg tttgctgtta
     1501 gtatccatag ggcagataaa ctggcaccaa aggcaaccag ataacaggct actaaatgga
     1561 actttttgct aatacgcccc caaccaaaaa acataacagc aacaaaagta gattcgagaa
     1621 agaatgccat aataccttca gccgctaagg gtgcaccaaa aatatcgcct gaaatccacg
     1681 aataattaga ccagttggtg ccaaactcta aacccaaaat gatgcctgtg cccacaccaa
     1741 tagcaaagtt gataccaaat aattttgccc aaaattttgc gagtttttcc cattcagggt
     1801 cattggttct tacgtggagg gtttgtgcaa aggcaatgat atacgtcata ccaatggtaa
     1861 gaggtataaa aagccaatga aaaaaggctg tcatggcaaa ttgggctcgc gaccagttta
     1921 ccagtgttaa gtcaatgttt tcaatcatac gaaatggtgt ttaatggttg tattaaatca
     1981 tgagtaactg attcgatgcg gtgttgttca ctttcccatt tgggtttaag ataacgagga
     2041 tacaaaaaac cttttagaaa tccataaaaa attaaaaatt tgataagaat gattatcaac
     2101 aatattttag ccatttttga ccgtttgatg tatagaatgc catctttgat aaaagaaaaa
     2161 tggctagaac ttggttgttg tatcatataa gtgttcaata ggtagcaaat atattaagaa
     2221 atgataaaaa aatcaaatag ttattacaaa aatgggtgtc ttgaagttga tttttaacta
     2281 aaaaacaata atttgcgaaa tatactgtat attcaatcct atcgtatgac attttttttt
     2341 gcaaattgtg tatctttgtt cacttacatt tgatttacag aatttacatc aaccatatga
     2401 cagacaaaga aattgtttta acgaaagaaa atttgtttta ttatttgagt aaatcccagt
     2461 tgggaagagc tatagagctg attgataatt tgttgacaca agtgcaagat ggttattatg
     2521 gcgatgaagt ttacgaactt aaaacaaact ataaacgtat gttgcagtat atgttttacg
     2581 gggttaatga tcctgaacaa catcttgttt atcagcattt actgaaagaa acgtacgaga
     2641 taggagagca tttagctgac aaacttttgc aacttaatag tacccaatat acatatcgta
     2701 ccaaacgttt atttgccgta caacctgtta tctcagatga gttggtattg tctggttttc
     2761 aacgctatgc atcactaggg aatcgtaaaa cggaaagtga tattcgtgaa tatgaaatgt
     2821 tgatttctta tagttttgat aagtattggt taacatctct gtatagcgaa actatgctac
     2881 aacaggtaaa actgttggca gaggatgaga ctattggtca aatcgaattg tgttcacttg
     2941 cttcagcaat tacattgagt agcatggctt tttttgacga aaaaaaatgt ttggcgttag
     3001 ttgaattaac aaaatcgcgt tatcctgaag ttgtattaag ggcattgaca ggcgtagtgt
     3061 tatttttgta ttttcatcat caacgcattt ctttttatcc cgttattcgt acccaaatta
     3121 ccttgttgtt tgataaagaa gagtttatcg tagcctcaca attaattata aaacaattta
     3181 ttagaagtaa agaaactgaa aaaataacaa aagatattac tgaaaatatt ctgccagaat
     3241 tatccaaaat agctccacat gtgcaaaatg atttagccga tattgataag caagaagagt
     3301 tggaagataa aaaaaatcaa tggcaagata tgatagaaga aacgggtatt ggtgaaaaaa
     3361 tgcaacaata tgccgaacta caaagtcaag gtgcagatat tcagatgagt acgttttcga
     3421 tgctcaaaaa ttacccattt tttaatgttg tttccaattg gtttttgcct ttttatgccg
     3481 aacattcttc tgtgagcaac ttatttaaag aagaaaagga aaatagtttt ttatcttttt
     3541 tagccaaaag tcctatactt tgtcattcgg ataaatattc gttttgccta aatttaacga
     3601 tggtgccaga gcaatatcgc aaagcaatga tgaacggtgt acaaatggaa tcggaacaat
     3661 taaaagaaat gacgaacgat aagctatctc tctctaatac ggttgttgcc gaagaaataa
     3721 tgaataaata cattcaagat ttatatcgtt tttacaaact gttccctaaa aataacaaaa
     3781 acagttttga cgatattttt acctataagt ttgattttca caaaacatgg atgtttcgtt
     3841 tactaaaccc aacaaacgaa tttttgtttg aaattgcaaa cttctatttt gcaaaagaaa
     3901 tgtatgaaaa tgccctttct gccttattgg aattagagga acatacagag ccctctgctg
     3961 acttgtatcg taaaattgga tttagctatc aaaaaaccta caattattcg ttagccattg
     4021 agtattacct aaaagcagaa ctgattgaaa ctgaaaatat atggacaatc cgcaaaatag
     4081 catattgtta tcgcaattta aaacaaacgg aaaaagcctt agagtattat ttgctggctg
     4141 atagtttgca acccaatact attcggttgt tgtttaccat tggtgtttgt tatgtagaaa
     4201 tgcaagatta tgatactgct ttgcgttatt tttataaaat tgagttttta gaatcgaacc
     4261 ataaaaagag cgaaaattat attgctttgt gttcattttt acaaaaaaaa tattctcagg
     4321 ctaccaaata ttaccataaa cagccagaca aaaatacctt ttatgaatta ctttacattg
     4381 gtcatgctta ttggtgttta ggaaataaaa aagaggcttt acactattat ctacaaagtg
     4441 cttatacagt aaaaaaagag actaaacaat ttaaaaactt ttatgaagcg ttggagcaag
     4501 ataccgatat tttgttgaaa catggttttt cgctcaaaga cattgttttt ataggcgacg
     4561 ctgtacgcat gaaaattgaa tcataacaca cgaatcattt aaccttatac acatgaaatt
     4621 agcattaaaa aaccccattg t
//
LOCUS       DAOSMY010000095         4633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_18608_length_4633_cov_2.651595, whole genome shotgun sequence.
ACCESSION   DAOSMY010000095 DAOSMY010000000
VERSION     DAOSMY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18608_length_4633_cov_2.651595"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..742)
                     /gene="rsmG"
                     /locus_tag="PLD76_03190"
     CDS             complement(122..742)
                     /gene="rsmG"
                     /locus_tag="PLD76_03190"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU27041.1"
                     /translation="MNVISKYFPNLSIQQVAQFEALFPLYMDWNSKINVISRKDIENL
                     YVHHVLHSLAIAKIIPFVDQTSVIDIGTGGGFPGIPLAILFPNVQFHLVDSIGKKIKV
                     AQEIAQEIGLENTTFLHDRIENEKKTVDFVVSRAVMPLGDLVRFCKKNIKKEQKNSLP
                     NGLICLKGGELQSEIQAFKKSATIYELTDFFNEDYFKTKKAVYIPL"
     gene            856..1314
                     /locus_tag="PLD76_03195"
     CDS             856..1314
                     /locus_tag="PLD76_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27042.1"
                     /translation="MKRFILQFFAVLLFFASLGVNVVHYGCDACREYFPILMGQSCMD
                     VHHSVKGDLAICCTSSCDDSEAQDIPHKHDDHHQDCTSQRLSIPLDSYIAKVQITQPL
                     STIPSFLLSAVLAIPSDIQQINGKKEYLSINLYKYYLYEDYLSKIAVFII"
     gene            1514..3634
                     /locus_tag="PLD76_03200"
     CDS             1514..3634
                     /locus_tag="PLD76_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU27043.1"
                     /translation="MHSQVIGKVLGENKEPLIGANIYWENTNYGVLSSIDGNFEIEKM
                     NTSDRLIISYLGYKSDTIQITYSSPYIVQLAQVTELDEVEVSGRKSGLVTFRATPIQT
                     QEISYSELCRAACCNLSESFETNPSVDVSYTDAATGAKQIRLLGLSGIYVQMINENIP
                     SFRGLGSTYGLSYVPGTWMDGILISKGTSSVSNGYESITGQINVEYKKPQLADPFSVN
                     LFGSSDGRVELNADASTKLTKNLSTMLFVHADKDFQHHDKNNDGFSDYPQVEQYSFFN
                     RWQYANKGYTAQFGAKGLYENRRGGEMIHHGYKINIDTWRADAFMKHGFVLNKEKESS
                     IGVIANGVFHRQESIYGIQTYDASQTSLYLNAIYQSHLTDEETHFIKTGLSMEADLYE
                     EKLNTQITETDYITPGIFGEYTLKLADKWIVLAGIRADYNKQYALFVTPRLHIKYMPI
                     NAVTLRFSIGKGYRSPNVMIENNNLLASNREFVISTPLQQEAAWNTGVNAVFDIPIGY
                     RTVMISTEYYYTLFDNQLVIDFDSFANKILFYNALGTSYAHNAQIEVSTEIADGLTIL
                     GAYRYNDTKSVTGGVLQLKPLMSMHRGLITASYKTSLGRWQYDITSQLNGGGRMPTPD
                     AINPLWNKTFSAYQQYNAQVTRYFKNASVYVGIENITNFTQLNPIISASNPQSDTFDA
                     TMIWGPLHGRKFYLGLRYNLPKQN"
     gene            3645..4001
                     /locus_tag="PLD76_03205"
     CDS             3645..4001
                     /locus_tag="PLD76_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOU27044.1"
                     /translation="MKNKVITTILSLLVFLPMLVAQQPKAEKISATFMVNIHCHSCKE
                     KITTAMQEEKGLKKIKINMKDKTVAITFMSDKNSVSNLLSVFKKLGYVATIQEVSCIG
                     SKEGCLNAIHPESTMR"
     gene            4119..>4633
                     /locus_tag="PLD76_03210"
     CDS             4119..>4633
                     /locus_tag="PLD76_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27045.1"
                     /translation="MKIYRFVLFILFFLSIKAYTQTSEDYFNSGFAKSQQLDFEAAII
                     DFTKAISLDATFAEAYNHRGIANRYLKKYQVAITDYTKAIEIHPHYAEAYNNRGVVKH
                     QIQLYQSALEDYNRAIQLNSMYAEVYNNRGVTKHVLRDFQSALADFTKAIDIDPKYAE
                     AYNNRGVTRFQ"
BASE COUNT         1575 a          690 c          792 g         1576 t
ORIGIN      
        1 aaatgtcgag gaactttgcc aattgcttgc aatacatgtg ttgagaaatt tcctttcatt
       61 gctagtattt ctactaactc ttttcttctt gcggcgtatt ttagttgatg cataataact
      121 tttacaaagg aatataaaca gctttttttg ttttaaaata atcttcattg aagaaatcag
      181 ttaactcata tattgttgca ctttttttaa aagcttgtat ttctgattga agttcccctc
      241 cttttaaaca tattaatcca ttagggaggg aatttttttg ttctttttta atattttttt
      301 tacaaaaacg aactaaatct cccagaggca taacagctct actaaccaca aaatcaactg
      361 tttttttttc attttcgatt ctatcgtgga gaaaggttgt attttcaagt ccaatttctt
      421 gagctatttc ttgagctact ttaatttttt taccgattga atcaactaaa tgaaactgta
      481 cgttaggaaa caatatagct aacggaatac caggaaaacc gccaccagtg ccaatgtcaa
      541 taacagatgt ttgatcaaca aaaggtataa tctttgctat cgctaatgaa tgtaatacgt
      601 gatgaacgta taaattctca atatctttac gtgaaatgac gttaattttg ctattccaat
      661 ccatatataa tggaaaaagt gcttcaaatt gagcaacttg ttgtattgat aaatttggga
      721 agtatttact aatcacattc atgcttaaaa aattaaaaat tcataatttt atgtcaaaga
      781 tacgtttttt ctatatctgt aaaaattttt tgtgtgtatc tttgtaaaaa agtaattaac
      841 actactttct tgtcaatgaa acgatttatt ttacagtttt ttgccgtatt actctttttt
      901 gctagtttag gagtaaatgt tgttcactat ggatgtgatg cttgtaggga gtattttcct
      961 attttaatgg gtcagtcatg catggatgta caccattcag taaagggtga tcttgctatc
     1021 tgttgtacta gttcttgtga tgatagcgaa gcacaagata tccctcacaa acatgatgat
     1081 catcatcaag attgtacatc tcaacgctta tctatacctt tggatagtta catagctaag
     1141 gtgcaaataa cacagccttt atctactatt ccgtcttttt tactttctgc tgttcttgca
     1201 ataccttctg atattcaaca aattaatggt aaaaaagaat acctttcaat taatttgtat
     1261 aagtactatt tgtacgaaga ttatttatcc aaaatagctg tttttattat ttgatttgaa
     1321 ctaaactcta gttttatcta tgtatcatca ttgtgtccat ttacgatata atgggttgta
     1381 ctcaagataa aagtaagaaa aaagggtgtg tttgttacac atttacgttt tttattttag
     1441 tttattcact tcattttttt taatactgaa aacattttat atctttttaa ttggattgct
     1501 ggtatctgca tccatgcatt ctcaagtaat tggcaaagtg ttgggagaaa acaaagaacc
     1561 ccttattggt gctaatattt attgggaaaa tacaaattat ggtgttcttt cttctattga
     1621 tggaaatttt gagatagaaa aaatgaatac atcggatcgt ttgattataa gttatttagg
     1681 atataaatca gacacaatac agattacata ttcttctccc tatattgtgc aattggcaca
     1741 agttacagaa ttagatgaag tagaggtttc gggcagaaaa tcaggcctag taacttttag
     1801 ggctacacct attcaaacgc aggagatatc ttattcagaa ttatgtcgtg ctgcttgttg
     1861 taatttgtct gagagttttg aaacaaatcc ttcggtagac gtctcttaca cagatgcagc
     1921 tacaggtgca aagcaaatta ggttgttagg tttatcaggt atttatgtac aaatgataaa
     1981 tgaaaatata ccaagtttta gaggattagg tagtacctat ggtttatcgt atgtgcctgg
     2041 tacgtggatg gatggtattt taatttctaa gggaacatcc tctgtatcta atggatatga
     2101 atcgattaca gggcagatta atgttgagta caaaaaacct cagttagcag acccattttc
     2161 agtgaatctg tttggtagta gcgatggtag ggttgaactt aacgctgatg caagcactaa
     2221 gctgacaaag aatttgtcaa cgatgctatt tgtgcacgca gataaagatt ttcaacatca
     2281 tgataaaaat aacgatggat tttctgatta tcctcaagta gagcaatatt cattttttaa
     2341 cagatggcaa tatgcaaaca agggctacac agcacagttt ggagctaaag gcttatacga
     2401 aaatagaaga ggcggagaaa tgatacatca tggctataaa atcaatatag atacttggag
     2461 agcagatgct tttatgaaac atggctttgt attgaataaa gaaaaagagt cgagtattgg
     2521 tgtgatagca aatggtgtat ttcatcgcca agaaagcata tatggaattc aaacatacga
     2581 tgcatcacaa acgtcattgt atctaaatgc aatttatcag agtcatttaa ctgatgaaga
     2641 aacgcatttc attaaaacag ggctaagtat ggaagctgat ttatatgaag agaagttgaa
     2701 tacccagata acagaaacgg actacattac tcctggtatt tttggtgagt atacattaaa
     2761 actggcagat aaatggattg tgttggcagg tatacgagct gattataata aacaatatgc
     2821 tctatttgta accccacgtt tgcatattaa atacatgcca ataaatgctg ttaccttgcg
     2881 tttttctata ggtaaagggt atcgatcgcc aaatgtaatg atagagaata ataacttact
     2941 tgcaagtaac agagagtttg ttatttctac tccactacaa caagaagctg cttggaatac
     3001 aggcgtaaat gcagtatttg acattccaat tggctaccga acagtaatga tttctacaga
     3061 gtattattat acattatttg acaatcaact tgttattgat tttgattcat ttgctaataa
     3121 aatccttttt tacaatgcat taggcacgtc ttatgcacac aatgcacaaa tagaagttag
     3181 cactgaaata gcggatgggt taacaattct tggagcttat cgatataatg atacgaaatc
     3241 tgtaacagga ggtgtattac aactaaaacc actaatgagc atgcatagag gtttaataac
     3301 agcatcttac aaaacttcct taggcagatg gcagtatgat attacctcgc aattaaatgg
     3361 tggcggacgt atgccaacgc ctgatgcaat aaatccatta tggaataaaa cgtttagtgc
     3421 ctaccaacaa tacaatgctc aagttactcg ttactttaaa aatgcatctg tgtatgtagg
     3481 tattgaaaat attactaact ttacacaatt aaatccaatt attagtgctt ctaatccaca
     3541 atcagataca tttgatgcta caatgatttg gggacctttg cacggaagaa aattttatct
     3601 gggtttacgc tacaatttac caaaacaaaa ttaaatatat aaatatgaaa aacaaggtta
     3661 taactacaat tttatctctg ttagtgtttt taccaatgct agtagcacaa caaccaaaag
     3721 cagagaaaat ttcagcaacg tttatggtaa atattcattg ccattcttgc aaagaaaaaa
     3781 ttacgacagc tatgcaagaa gaaaaaggat taaaaaaaat taaaattaat atgaaggata
     3841 aaacggttgc aattactttt atgtctgata aaaattcagt aagtaattta ttgtcggtat
     3901 ttaaaaaatt aggttatgtt gctactattc aagaggttag ttgtataggg agtaaggagg
     3961 gttgtttaaa cgcaatacat cctgaatcaa ccatgaggta attagtaata aaaaaagtat
     4021 cacattctat tatttgtgat acttttttta ttacatttac gtttaattaa taatagcact
     4081 ttaaagtgct attatcagta acctgtaaaa tttgcattat gaaaatatat agattcgttt
     4141 tatttatact tttttttcta tctataaaag cttatactca aacgtcggaa gattatttca
     4201 atagtggttt tgccaaatct caacaattgg actttgaagc ggctataatt gattttacaa
     4261 aagcaatttc tttagatgcc acatttgctg aagcatataa tcatcgaggc attgccaata
     4321 gatatttaaa aaaatatcaa gttgcaataa ctgattacac aaaagcaatt gaaatacatc
     4381 cccattatgc cgaagcttac aataatagag gagtggttaa acatcagata caattatacc
     4441 agagtgcatt ggaggattat aatagagcta ttcaacttaa cagtatgtat gctgaagtat
     4501 ataacaatag aggcgttaca aaacacgtgt tacgtgattt tcagtcggca ttggctgatt
     4561 ttaccaaagc tattgatatt gatcctaaat atgccgaagc ttataataac aggggggtaa
     4621 ctagatttca gtt
//
LOCUS       DAOSMY010000096         4575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_18948_length_4575_cov_3.002876, whole genome shotgun sequence.
ACCESSION   DAOSMY010000096 DAOSMY010000000
VERSION     DAOSMY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18948_length_4575_cov_3.002876"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLD76_03215"
     CDS             <1..205
                     /locus_tag="PLD76_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HOU27046.1"
                     /translation="IMIIELKQDSSSASMMKSILMDLRIQPKSISKYCLGTVLTNPAA
                     KYNWFKKKLMYINKIRQQQNHTL"
     gene            202..915
                     /locus_tag="PLD76_03220"
     CDS             202..915
                     /locus_tag="PLD76_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOU27047.1"
                     /translation="MNDLTTFDPSIAAEFGENSTIDFLGVALFDATSLLHLLMRFVFN
                     MLVCWVIVQFFYYKKSKRRDYYVTFIMFSTTMFLLIFLMKNVDLHIGLTLGIFAIFGM
                     MRYRTETVPIREMTYLFVITGISVINGLSMTVSYAELVATNLLFVILAYIIENQKVLK
                     HTSSKLVLYEKIDLIVPEKREELKADLEKRTGLKIEKLEIGHIDFLRDVAFVKIYYVL
                     SPGESNSIDSIGKINQYVG"
     gene            905..1690
                     /locus_tag="PLD76_03225"
     CDS             905..1690
                     /locus_tag="PLD76_03225"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOU27048.1"
                     /translation="MLGKRNVRFLSILFLGILFVSQVYSQTITYDETEDTDVGLRLSA
                     EISKRLSRNWSISLEEELRLNDNISRFDRLYSSVSVGYTINPYINTSIGYQHRLIQQK
                     GKASTNYQGYLDFRHRGILSVSFSYPVGQFEFSLRQRLNGTLRTDSVNPLEKSNPQII
                     SRTRMQAEYSFWSIPLKPYVSVEMFNPLNNPAYISDVWLETMRYTGGFTYRLDARSRI
                     ELYYFYEDEMGKDVNIGRNSGRVTITTEKEHNHVLGIAYQYRF"
     gene            complement(1687..3705)
                     /gene="uvrB"
                     /locus_tag="PLD76_03230"
     CDS             complement(1687..3705)
                     /gene="uvrB"
                     /locus_tag="PLD76_03230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU27049.1"
                     /translation="MDFELVSPYKPTGDQPQAIEQLTSGILGGISDQTLLGVTGSGKT
                     FTIANVIEQVNKPTLVLSHNKTLAAQLYREFKSFFPNNAVEYFVSYYDYYQPEAYLPT
                     TDTYIEKDLAINDEIERLRLSTTSSLLSGRQDIIVVSSVSCIYGIGNPDDFTNNVINI
                     KQGEQIVRDMFLRQLVSSMYNRNEIDFQRGNFRVKGDTVDIWLAYADIMLRVIFWGNE
                     IESITTHDPLHLFTLEHHDAYKIFPATIFVTTKDSIHRAIGEIEIDLGKHVDYMKSIG
                     KHYEAKRLYERVTYDLEMIRELGYCSGIENYSRYFDGREPGSPPFCLLDFFPKDFLMV
                     IDESHVTLPQIRAMYGGDAARKKNLVEYGFRLPAAMDNRPLTFDEFNEHKKQTIYVSA
                     TPADYELERCNGIVVEQVIRPTGLLDPIIEVRPSLNQIDDLQEEIVQRVQKQERVLIT
                     TLTKRMAEELHLYLQRAGVRCTYIHSDVDTLERVEILDHLRSGFYDVLIGVNLLREGL
                     DLPQVSLVAILDADKESFLRSHRSLTQTAGRAARNLNGKVIMYADKITESMRKTIDET
                     NRRREKQLAYNAEHNITPTAIAQHAHATLKNKELYTDHPTYSMDNSMVDVAADPIVQY
                     MNKTQLQKSIEKTKKQMQEASKNMEFLEAAQYRNELAKLQEILKSKNS"
     gene            complement(3802..>4575)
                     /locus_tag="PLD76_03235"
     CDS             complement(3802..>4575)
                     /locus_tag="PLD76_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU27050.1"
                     /translation="IRNMCMLKILIIEDEKPALALLEKMLAQTINNFTIIGRCDSIES
                     SVTFLKKHQDIDLIFMDIHLGDGNSFDIFLQIQIDTPIIFTTAFDMYAIKAFELNIID
                     YLLKPIKKEDLTRSIEKFYKSKKHLQQFDIQYLSSIISNRSNIYKERFLVYIGNNIKA
                     IMTSDIAYFYSLDSATYLVTHNGNTYDVNMSLEQLIGVLDPQIFFRVNRQYIVAFSAI
                     DKIQLLSTNRIKIVLLPPTDKEILVSSNKNQLFKEWLNR"
BASE COUNT         1436 a          725 c          831 g         1583 t
ORIGIN      
        1 tataatgatt attgagttga aacaagacag ttcgagtgct tcgatgatga aatctatatt
       61 aatggattta cgcatccaac ccaagagcat tagtaaatac tgtttaggca cagtgcttac
      121 caaccctgct gccaaatata attggtttaa aaagaaatta atgtatataa ataaaataag
      181 acaacaacaa aaccacacat tatgaatgat ttaacaacat ttgatcccag tattgctgcg
      241 gaatttggcg aaaattctac cattgatttt ctaggtgtag ctctatttga tgctaccagt
      301 ttattgcatt tactaatgcg ttttgttttc aacatgttag tatgttgggt gatagtgcag
      361 tttttctatt ataagaaaag caaaagacgt gattattatg ttacgtttat tatgtttagc
      421 actaccatgt ttttgttgat tttcttaatg aagaatgttg atttacatat tggattaaca
      481 ctaggtatat ttgccatttt tggcatgatg aggtatagaa ccgaaacggt tcctatccga
      541 gagatgacct atttatttgt tattacaggt atttcggtta ttaatggttt gtcgatgact
      601 gtcagctatg ctgaattagt tgctacaaat ttattatttg ttattcttgc atatattatt
      661 gagaatcaaa aagtattaaa acatacatcg tcaaaattag tgttgtacga aaaaattgat
      721 ttaatagttc ctgaaaaaag agaggaacta aaagccgatt tagaaaaacg aacaggatta
      781 aagattgaaa aattagaaat aggtcatatt gattttttac gtgatgtggc ttttgtaaaa
      841 atatattatg tattaagtcc tggtgaatca aatagcattg attcaattgg aaaaataaat
      901 caatatgttg ggtaaacgca atgttagatt tttaagcata ctctttttag gtatattatt
      961 tgtttcgcag gtgtattctc agactataac gtacgacgaa acagaagaca ctgatgtggg
     1021 gttgagattg tctgctgaaa tatcaaaaag attaagtcgt aattggtcaa tatcgttaga
     1081 ggaggagcta cggttgaatg ataacattag tcgattcgat aggttatatt catctgtatc
     1141 tgtgggttat acaattaatc catatattaa tacgagtatt ggctatcaac ataggttaat
     1201 acagcaaaaa ggaaaagcga gtactaatta tcaaggatat ttagatttta gacacagagg
     1261 aatattgagt gtttcatttt cctatccagt tggtcaattt gaattcagtt tgcgacaacg
     1321 attaaacggt accttacgta cagattctgt taatccctta gaaaaatcaa atccacaaat
     1381 catttctcgt actcgaatgc aggctgagta cagtttttgg agtattcctt taaagccata
     1441 tgtttctgta gaaatgttta atccgttgaa caatcctgct tatattagtg acgtttggtt
     1501 agaaacaatg cgatacacag gaggttttac ttatcgcttg gatgcgagaa gccgaataga
     1561 attgtattat ttttatgagg atgaaatggg taaggatgtg aatataggac gcaatagcgg
     1621 gcgagtaacc ataacaaccg aaaaagagca taaccatgta cttggaattg cttatcaata
     1681 taggttttag ctattctttg atttcaaaat ttcttgtaat tttgctaact cattacgata
     1741 ctgggcagcc tctaagaatt ccatattttt agaggcttct tgcatctgtt tttttgtttt
     1801 ttctatagat ttttggagtt gtgttttgtt catgtattga acaattgggt cagcagcaac
     1861 atctaccatg ctattgtcca ttgagtatgt tggatggtct gtgtataatt ctttgttttt
     1921 taacgttgcg tgtgcgtgtt gagctattgc agttggtgta atattatgtt cggcattgta
     1981 cgcaagttgt ttttctcttc gtctgttcgt ttcgtcgatt gttttccgca tgctttcggt
     2041 tattttgtca gcatacataa tcacttttcc gttgaggttg cgtgcggctc taccagctgt
     2101 ttgggtaagc gagcgatgcg aacgaagaaa gctttcttta tcagcatcaa gaatagcgac
     2161 taacgatact tgtggcaaat caagcccctc tcttagtaag tttacaccta ttaatacatc
     2221 ataaaatcca gaacgtaagt gatctaatat ttccaccctt tcaagcgtat ccacatcgga
     2281 gtgtatgtat gtacatcgta cccctgcacg ttgtaggtag agatgcaatt cttctgccat
     2341 acgttttgta agagtggtaa ttagtacacg ttcttgtttt tgtacacgtt gtacaatttc
     2401 ttcttgtaaa tcgtctattt gattcaaact aggacgcact tcgatgatgg ggtcgagcaa
     2461 accagttggg cgaattactt gttctacaac aataccatta caacgctcta attcgtaatc
     2521 agcaggagta gcacttacgt aaatagtttg ttttttatgt tcgttaaatt catcaaatgt
     2581 aagaggtctg ttatccatgg ctgcaggtaa tcgaaagcca tattcaacaa ggttcttttt
     2641 gcgagcagca tcgccaccat acatggcacg tatttgaggg agtgttacgt gactttcgtc
     2701 tataaccatt aaaaagtctt ttggaaagaa atctaacaga caaaacggag gagaaccagg
     2761 ttctctacca tcaaaataac gagaataatt ttcgatgcca gagcagtagc ctaactcccg
     2821 tatcatttct aagtcgtaag ttacacgttc gtataatcgt ttggcttcgt aatgcttgcc
     2881 aattgatttc atataatcaa catgtttccc caaatcaatt tctatttcgc caatagctct
     2941 atgaatacta tctttggtag taacaaaaat agtagcagga aatattttat aagcgtcgtg
     3001 atgctctagt gtgaataagt gcaatgggtc gtgggtagta atgctttcta tttcgtttcc
     3061 ccagaaaatg acacgtaaca taatgtcagc atacgcaagc caaatgtcga cagtatctcc
     3121 ttttacccta aaattacccc gctgaaagtc aatttcattt cggttataca tggatgatac
     3181 taattgtctg agaaacatat cacgcactat ttgttctcct tgtttgatgt taattacatt
     3241 attcgtaaag tcatcaggat taccaatacc atagatacac gaaacggaag aaacaacaat
     3301 aatatcctgt ctgccagaaa gtagagatga agtagtagag agcctaagac gctctatttc
     3361 atcatttatg gctaaatctt tttctatata ggtatcagtg gtcggtaagt atgcttctgg
     3421 ctggtagtaa tcgtagtacg aaacaaagta ctcaactgca ttgttcggaa aaaaactttt
     3481 aaattctctg tataattgag cagctaaagt tttattgtga cttaaaacga gagtaggttt
     3541 atttacttgc tcaatcacat tggctatggt aaatgtttta cccgaaccag taacccctaa
     3601 taaggtttgg tcgcttattc cacccaaaat gccactagtt aattgttcga tagcttgagg
     3661 ttggtcgcca gttggtttat aaggagaaac gagttcgaaa tccatagata agattattta
     3721 cgcgaatgta atgacttata ttgatacaag caaatataag aatagataga taacaatttt
     3781 ttcagttata aaaactattt ttcatctatt taaccattct ttaaacaact gatttttatt
     3841 gctacttaca agtatttctt tatctgtggg gggcaataat actattttaa ttcggttggt
     3901 agaaagtaat tgtatcttgt cgatagcact gaatgcaaca atgtattgtc tgttaactct
     3961 aaaaaatatt tgtgggtcta aaacaccaat caattgttcc aacgacatat ttacatcata
     4021 agtattgcca ttgtgtgtta caaggtatgt ggcactgtca agtgaataaa aataggcaat
     4081 atcacttgtc ataattgctt ttatattgtt tccgatatac accaaaaacc gttctttata
     4141 aatattagaa cgattagaaa taatggaaga taaatattga atatcgaatt gttgtaaatg
     4201 ttttttcgac ttataaaatt tctcaattga tctagttaaa tcttcttttt ttatgggttt
     4261 caacaaataa tcaatgatgt ttagttcgaa tgcttttatt gcatacatat caaaagcagt
     4321 agtaaaaatg ataggtgtat caatctgtat ttgtaagaat atatcaaaac tattgccatc
     4381 tcccaggtgt atatccataa aaattaaatc aatatcttgg tgcttcttca agaaagttac
     4441 gctcgactca atgctatcac atcttccaat aattgtaaaa ttattgattg tttgagccag
     4501 cattttttct aacaatgcca aggcgggttt ttcatcttca atgattaata ttttcaacat
     4561 acacatattt cttat
//
LOCUS       DAOSMY010000097         4484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19467_length_4484_cov_3.028900, whole genome shotgun sequence.
ACCESSION   DAOSMY010000097 DAOSMY010000000
VERSION     DAOSMY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19467_length_4484_cov_3.028900"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLD76_03240"
     CDS             complement(<1..834)
                     /locus_tag="PLD76_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27051.1"
                     /translation="MKISAFIPYENDEQVAQTIASLQGSNLIGKIFLLATKQASTSTK
                     FDAQTLTIDTLTSTATMRLIADNCTNDFALIYLKYTSLYMGAYAIERLAQVANDTQAG
                     MVYTDYYQQTTTGTAKVPVNDYQIGSVRDDFNFGSLLFFATKVLQSSVASCSKDFAHA
                     GLYQLRLTVSEQSQLVHLNEFLYTEIEQDTRKSGEKQFDYVDPKNRAVQIEMEQVCTQ
                     HLKNIGAYLTPIFPAVNLHQDLFAYEASVIIPVKNRVKTIEDAIVSVLKQETNFPFNL
                     LI"
     gene            complement(857..3811)
                     /locus_tag="PLD76_03245"
     CDS             complement(857..3811)
                     /locus_tag="PLD76_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HOU27052.1"
                     /translation="MMQAYFRWHYIILFVSCWIFSTSMLAQAKFKLQTIRYTPEITAK
                     KTVYSVSKQTLKNNTLTLWVENDVANIPFREESIQAFYDSVYAQLPATYREYQLEIIC
                     NNRTLDELVPNAYRKTIAIDNARKFTPPHITPVIQNVSKPYTPTQGLLHRHIALWNSH
                     GWYYSAITDKWDWQRGRLFGTVEDMLTTAIVLPYVLPMLENAGANVFIPRERDTQTNE
                     VILDNDDKSTNESITHTTSWEKKETGFKNKKLLQGRDNPFTMGSFLQTPTYEEATDSI
                     EWKIKVPKNGMYYVSIAYKSVSNSTESARYSLHHAGGTTRFSVNQQLGGGTWIYLGNY
                     YFIANKSYSLVLTNSGKEGYVVTADVVRVGGGMGNIDRQTTESIVEKKLFPPFIDEDA
                     ISQPPLISTPRARFTEAARYYLQWAGMPDSILINQRKDVISDYQDDIFGRANWVNYLM
                     GGSVMYPSYKGLGIPIDACIALHTDAGKMSGDSIVGTLGIAMTTDDKGMYPANFSRIA
                     ARDFTDMVQTELVHDIRATFPVNWTRREIWDKSYIEARIPKTPTTIIELLSHQNFNDM
                     RYAHCPEFRFVTARAIYKGIVKFLASQYNQPYTIQPLPITHFVAEVLQDDSVHLSWRK
                     TTDPLEPTANPDGYIVYTRKNDGGFDNGIVSKTESITLPIEKGTIYSYKIAALNNGGE
                     SFASEILSVYKAEIEKGKAWVVNAFDRVAAPSSFETDSTAGFTHATDRGVPYLYDLSF
                     TGNQIEYNKSVPFYDNDYSGFGASQANYDGQVIAGNTFDNCYIHGRALAANNYSFSSA
                     SRDGITVLSPSFTGYDVLAIILGNQQTSKTFTRTFTVYPTDLQTLITNYSKQNGKIII
                     SGSHIASDITRKKDKSEMQFAQNILKYKLRMPQASVNATMVDVFSAKKRQREYNCFTQ
                     PNPYQYAVQQVDAIQPTDKNSSVLLRYTENNTAAAIGYKGKYKTIVCGFPFETLTSTE
                     QQNELMRLFVNY"
     gene            3955..>4484
                     /locus_tag="PLD76_03250"
     CDS             3955..>4484
                     /locus_tag="PLD76_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOU27053.1"
                     /translation="MKLLADSGSTKTAWVVLNENGSNSVEYITDGLNPFFQTVQSVQQ
                     TIQNQLLPHIDANSISEIYFYGAGCAFPEKNAIIEEGLRTVFASATIHVASDLLGAAR
                     GLCGMQDGIACIMGTGSNSCMYQDSKIVHNVSPLGYILGDEGSGAVLGKLLVGDYLKN
                     QMPTHLASAFLATYQLT"
BASE COUNT         1253 a          809 c          923 g         1499 t
ORIGIN      
        1 aatgagcaaa ttgaatggaa aattcgtttc ttgttttaac acagaaacaa tggcatcttc
       61 tattgtttta accctatttt ttactggaat aattacagag gcttcgtatg cgaataagtc
      121 ttggtgcaaa ttaactgctg gaaaaatcgg cgttaagtaa gctccaatat ttttaaggtg
      181 ttgtgtacac acttgttcca tttcaatttg taccgctcgg ttttttgggt caacgtaatc
      241 aaattgtttt tcgccacttt tgcgggtatc ttgttctatt tcggtatata agaattcgtt
      301 taagtgtaca agttgtgatt gttccgatac ggttaatcgg agttgataca gcccagcgtg
      361 ggcaaaatct tttgagcagc tagcaacaga tgattgcaat acctttgtcg caaaaaacaa
      421 taatgaacca aaattaaaat catcgcgaac actcccaatt tggtagtcgt taaccggaac
      481 tttagcagtg cctgtggtcg tttgttggta atagtctgta tataccatac ctgcctgcgt
      541 gtcgtttgct acttgtgcca atcgttctat agcataagct cccatgtata aggatgtata
      601 ttttaggtag ataagggcaa aatcattggt gcaattatca gcaattaacc gcattgttgc
      661 cgtactggtt agggtgtcaa ttgttagtgt ttgtgcatca aattttgtgc tagttgatgc
      721 ttgttttgtt gctaacaaaa aaatcttacc tatcaaatta cttccttgta acgaagcaat
      781 tgtttgagct acttgttcat cattttcgta agggataaaa gctgaaattt tcatattctt
      841 gatgttatat tgatgattaa taattgacga ataatcgcat tagttcgttt tgttgttcag
      901 tacttgtgag tgtttcgaaa gggaaaccac aaacaatggt tttgtatttt cctttgtacc
      961 caatggcagc cgctgtattg ttttcggtat aacgtagtaa aacggaacta tttttatctg
     1021 ttggttgtat ggcatctact tgctgcacag catattgata ggggtttggt tgggtaaaac
     1081 aattgtactc acgctggcgt ttttttgcag aaaacacgtc aaccatcgtt gcatttacgg
     1141 aagcctgtgg catgcgtagt ttgtatttga gtatgttttg tgcaaattgc atttcgcttt
     1201 tgtctttttt tcttgttatg tcagaagcaa tgtgtgatcc gctgatgata attttgccgt
     1261 tttgtttgct gtaattcgta attagtgttt gcaagtcggt aggatataca gtaaaagtac
     1321 gtgtaaacgt tttactcgtt tgttgattac caagtataat agcgagaaca tcgtatccag
     1381 taaatgaagg agacaatacc gtaatgccat cgcgtgaagc tgacgaaaat gaatagttgt
     1441 ttgccgctaa agcccttcca tgaatgtagc aattatcaaa cgtattccca gctattactt
     1501 gtccatcgta gtttgcttgg ctagcaccaa atccagaata atcgttgtcg taaaacggaa
     1561 ctgatttatt gtattcaatt tgattgcctg taaaactcag atcgtacaaa tagggaaccc
     1621 ctctatccgt agcatgagta aaacctgcgg tactatctgt ttcaaaagaa gaaggtgcag
     1681 caaccctatc aaaagcattt actacccaag cctttccttt ttcaatttct gctttgtaca
     1741 ccgataaaat ttcagaagcg aaactttccc cgccattatt tagagctgct attttataac
     1801 tatagatagt ccctttttca attggtagcg taatgctttc ggttttgctg acaattccat
     1861 tgtcaaatcc tccatcgttc ttgcgtgtat atacgatata accatcagga ttagctgttg
     1921 gttcaagcgg gtcggttgtt tttcgccaag ataagtgtac tgaatcatct tgtaacacct
     1981 ctgccacaaa atgagttatg ggtaagggtt gaattgtata aggttggttg tattgagatg
     2041 ctaaaaactt aactatcccc ttgtatatag cacgagccgt aacaaaacga aactcagggc
     2101 aatgagcata ccgcatatcg ttaaaatttt ggtgcgataa taattcgata atggtagtag
     2161 gagtttttgg tatgcgtgct tcgatatagg acttatccca aatttctctg cgtgtccagt
     2221 ttacgggaaa agtagcacgt atatcgtgta caagttccgt ttgaaccata tccgtaaaat
     2281 cgcgtgctgc tatacgcgag aagttggctg gatacatgcc tttatcatcc gttgtcatgg
     2341 caatgcccaa ggtgccaaca attgaatcgc cactcatttt tccagcatcg gtatgcaagg
     2401 cgatgcacgc atcaatgggt atgccaagtc ctttgtagct tgggtacata accgaacctc
     2461 ccatcaagta atttacccag ttggctctac caaaaatatc atcttgataa tcacttatta
     2521 catctttgcg ttggttgatg aggatactat cgggcatgcc agcccattgt aaataatagc
     2581 gagcggcttc cgtaaaacgt gctctaggag tagaaataag gggtggttga gaaatagcgt
     2641 cttcgtcgat aaagggagga aatagctttt tttctacaat gctttcggtt gtttgcctat
     2701 ctatatttcc catgcctcca cctacacgta caacatctgc tgttactaca taaccctctt
     2761 tgccagagtt ggttaacacc aaagaatagg atttatttgc tataaaataa tagttgccta
     2821 agtaaatcca agtgccaccg cctagttgtt ggttcacaga aaatcgtgta gttccaccag
     2881 catgatgcag gctatagcga gcagattcag tactgtttga aacgcttttg taggcaatag
     2941 acacgtaata cataccattt ttgggaacct taattttcca ttctatgcta tctgttgctt
     3001 cttcgtaagt gggtgtttgt aaaaaagaac ccatcgtaaa aggattatct ctgccttgta
     3061 gtagtttctt gtttttaaac ccagtttctt tcttttccca acttgtggta tgtgtgattg
     3121 attcattggt tgatttatca tcattatcaa gtatcacctc attggtttgt gtatcgcgtt
     3181 ctcgtggaat aaatacattg gctcctgcat tttctaacat aggcaataca tagggcagaa
     3241 cgatagctgt tgtaagcata tcttcaaccg taccaaataa tcgtcctcgt tgccaatccc
     3301 acttatctgt aatagcactg taataccagc catgactatt ccatagtgca atatgtctat
     3361 gtagtaaacc ttgtgtagga gtgtacggtt tagatacatt ttgtattacg ggtgtgatgt
     3421 gcggtggtgt aaattttctc gcattatcta ttgcgatagt ctttctgtaa gcattgggaa
     3481 ccaattcatc caaggtgcga ttattgcata taatttcaag ttgatactct cggtaggttg
     3541 ctggcaattg tgcgtacact gagtcgtaaa aagcctgaat gctttcttct ctaaatggga
     3601 tatttgctac atcattctct acccaaaggg ttaaggtgtt gtttttgagt gtttgttttg
     3661 aaacagaata aactgttttt tttgctgtaa tctcaggggt gtaacggatg gtttgaagct
     3721 taaacttagc ttgtgccagc atcgaagttg aaaatatcca acaactaaca aacaggatta
     3781 tatagtgcca acgaaaatag gcttgcatca tctaggtacg tggtttatta tatggttcaa
     3841 aaatacactt ttttttttat ttttcagcag agacaaacgt aaaatagtat aatttttact
     3901 tactttgtgc aagcgaaaag agatacgtat agaaacataa aaataagcga taagatgaag
     3961 ttactagcag atagcggttc cacaaaaacg gcttgggttg tgttgaatga aaatgggtca
     4021 aactcagttg aatacataac agatggctta aatccttttt ttcagacggt acagagtgta
     4081 caacaaacca ttcaaaatca actacttccc catatagatg ctaacagcat atccgaaatt
     4141 tatttttatg gagcaggttg tgcctttcct gaaaagaatg ctattattga ggagggactt
     4201 agaaccgtgt ttgcgagtgc aactatccat gtggctagtg atttgttagg tgctgctcgt
     4261 ggcttgtgcg gtatgcaaga tggcatagct tgtattatgg gcactggttc taattcgtgt
     4321 atgtatcaag atagtaagat tgttcacaat gtgtcgcctc tgggatatat actaggagat
     4381 gaagggagtg gggcagtatt gggtaaattg ttagtaggag attacttaaa aaatcaaatg
     4441 ccaactcatc ttgcttctgc ttttttggct acctaccagc ttac
//
LOCUS       DAOSMY010000098         4480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19484_length_4480_cov_2.756158, whole genome shotgun sequence.
ACCESSION   DAOSMY010000098 DAOSMY010000000
VERSION     DAOSMY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19484_length_4480_cov_2.756158"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    362..371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<372..840)
                     /locus_tag="PLD76_03255"
     CDS             complement(<372..840)
                     /locus_tag="PLD76_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU27054.1"
                     /translation="MKKVMLLVLGFLLGLNIIAQNEKPFYLGLYASPSVLWLQSDNNT
                     VDASGSKLGFSYGLLADITLGDYYALASGLEITQLGGTLSDQTVDYKLQYLEIPLALK
                     MQTASINNLSFFGRFGGGFGVRLDAKKDVNNTIINQPASDEIHLLRAYFSVGGG"
     gene            1156..2328
                     /locus_tag="PLD76_03260"
     CDS             1156..2328
                     /locus_tag="PLD76_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27055.1"
                     /translation="MNWPAESWSSAMNLTSKLTQTNVTELSGLHWNPATQCLYVVQDN
                     GRLRILQFNEGVDDFFEIANVEIAGNPEGIMQVDYEASMFYTVDEKNYEIRQYVPNQD
                     YTSITLMKKWRLLTPNTPMPDTGNDGIEGIVFVPDKHLQSIGFVSSQTGLPYTSTKGM
                     GGLIFIAHQTEGYVWVYDVNPALSFDVAYVGKYKTNRKESCDIAFDRTTGLLYILHNV
                     GKNFLEVTNLSLEQVNDEYKFVVSKEYSLSNPTDGNENIEGFAITPMCEENGVVSVFV
                     CRDVNDTGTEIQKGDALRWFTDFPLLGSCFPITEDEQIQTDNARFTIEFTSLSGLYIH
                     GAQENVELVFMDELGRIRYKTHYEPMSYLPYDVLPQGYSILQIHTSNDIQVVLIRL"
     gene            2358..2741
                     /locus_tag="PLD76_03265"
     CDS             2358..2741
                     /locus_tag="PLD76_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27056.1"
                     /translation="MKKKMSVLIVSLMLTHVMLATVHIHYLQKSVYETGRQQWGDWQN
                     EANAFCIDSTTLSIVNLSTKSETVYVRQGEAIGGYNERGNYYRLFEAQNEKGQKCLFF
                     LYSDRNVGLLFKCEEAGVIIQYAGY"
     gene            complement(2873..3436)
                     /gene="efp"
                     /locus_tag="PLD76_03270"
     CDS             complement(2873..3436)
                     /gene="efp"
                     /locus_tag="PLD76_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU27057.1"
                     /translation="MATTADIRNGMCIEFNNGLYTIVEFLHVKPGKGAAFVRTKMKNV
                     QTGRILENTFNAGVKLDEVRIERRPYQFLYKDDMGYNFMHNETFEQISIDKDKINGVD
                     FLKEGDIAEVTVHADTETILFADLATTTILEVTYTEPGLKGDTATNTLKPATVETGAT
                     VRVPLFINVGDKIKINTTEGSYVERAK"
     gene            complement(3515..>4480)
                     /locus_tag="PLD76_03275"
     CDS             complement(3515..>4480)
                     /locus_tag="PLD76_03275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27058.1"
                     /translation="IKQKINNFNPLEWEKVIAEIKKEQFELIIYGYADIASTLCISYI
                     SNRLKKMAIQVAVMHNVEINHPFEGIKKMATTMMFKQLDGFISMSNKVTEELKPFNKK
                     HRPVIFSPHPIYDLFGKGTDKKLALLQLGLNPNYQYVLFFGAIKESKGLDLLIEAFAD
                     ERVRQLPIKLLVVGDFNNNKEFYTDIMYENRLKNNIIFVDEYVPNHVVSLYFSACSIV
                     GQPYREVNQSGILQVAYHFDKPILITNIAQCGINIPNQKVGYVINPNTTEIADSLVDF
                     FVNKREEEMVNNVKIEKKKFMWNNMTEAIYSLYQTAKAYKNTRLI"
BASE COUNT         1418 a          687 c          756 g         1609 t
ORIGIN      
        1 attcaactat attttaatat attttcttaa atctttattt acgtaaatag ttgatgttca
       61 gtaggtataa atacattggt aaattgtagt aaattatggt tatagaaccc ccgcttccgc
      121 gaaagtcctt tgaaagaagg acttttttta tgtttaccta cttttttcac atctacaaat
      181 aaataagaac ggaatttagt taaaccgttc ttatgtgtat ccattttata ataaagagtt
      241 cgttaaaaaa taacaccaac attcaatgat aaggcattgc tttttacact ccaatcttct
      301 tgtttaaaag catttgttaa tccatttttg tagaaaatac ctgcgaaaat tttagtgttg
      361 tnnnnnnnnn ncacctccac caacgctaaa ataggcacgt aagagatgta tttcatcact
      421 tgcaggttgg ttgataatgg tgttatttac atcttttttg gcatctagac gtactccaaa
      481 cccaccacca aaacgaccaa aaaaacttaa attattgatg gatgcagttt gcatttttag
      541 ggctaagggt atttccaaat attgtaattt gtaatcaact gtttggtccg aaagagttcc
      601 tcctagttga gttatttcta aaccactagc aagtgcataa taatctccta aggtgatatc
      661 tgcaagcaat ccgtatgaaa acccgagctt tgacccacta gcatccaccg tgttattatc
      721 tgattgtaac cataaaacag aaggggatgc atatagccct aaataaaatg gtttttcgtt
      781 ttgagctatg atatttaatc ctaataagaa gcctagtaca agtagcatta cttttttcat
      841 atctgtatct ttttgttgaa aacaataatt atcaatacac aaatatacta aaaaatacag
      901 taatacaagg ataagtggtc ttttttttat atttttttta aagctattta gataggatat
      961 atcttgcttt attttgtgaa tatttttttt cttggattat tctatattta tatcttatct
     1021 ttgctattat cttaactatt cttattcaat taatctgata tgttatcctt gcctcaatat
     1081 agacaatccc ataaagttgt tttctacgcc ttaatgcaca tacttatttt accatttcct
     1141 ttgttttctc aaacgttgaa ttggccagct gagtcatgga gtagtgcgat gaatttaaca
     1201 tctaagttaa cacaaacgaa tgtaacagag ttgagtggct tacattggaa tcctgctaca
     1261 caatgtttgt atgtagtaca agataatgga cgcttacgga ttcttcaatt caacgaagga
     1321 gttgatgatt tttttgagat tgctaatgtt gaaatagcag gaaatcctga agggataatg
     1381 caggtagatt atgaggcaag tatgttttat acagttgatg aaaaaaatta tgaaatacga
     1441 caatatgttc ctaatcaaga ttatacttct attactttaa tgaaaaaatg gcgattgtta
     1501 acccccaata cgcccatgcc tgatactgga aacgatggta tagaaggaat tgtatttgtg
     1561 ccagataagc atttgcaatc gattggattt gtaagctctc aaacaggctt gccgtacact
     1621 tctactaaag gtatgggcgg attaattttt attgcacatc aaacggaagg ttatgtatgg
     1681 gtatatgatg ttaatccagc cttgtctttt gatgtagcgt atgtgggcaa atacaaaaca
     1741 aaccgtaagg agagttgtga tattgctttt gatcgtacca caggattgtt atatatatta
     1801 cataatgtag ggaagaattt tttggaagtt accaatttat ctcttgaaca ggtaaacgat
     1861 gagtataaat ttgtcgtatc aaaggaatat tcactgtcga accccacgga tgggaatgaa
     1921 aatattgaag gttttgcaat tactcccatg tgtgaagaaa atggagtggt ttcggtgttt
     1981 gtatgtcgag atgtgaatga tacaggtaca gaaatccaaa agggagatgc cttacgatgg
     2041 tttacggatt ttcctttatt aggaagttgt tttccaataa cagaagatga gcaaattcaa
     2101 acagataatg cgagattcac tattgaattt acttcattaa gtggcttata catacatggt
     2161 gcccaagaaa atgtagagtt agtttttatg gatgaattag gaagaatacg atataaaact
     2221 cactatgaac ctatgtctta tttgccatat gatgtattac cccaggggta tagtatattg
     2281 caaatacata catcaaatga tattcaagtt gttttgattc gattgtagtt gtttgtttaa
     2341 ttataaaaat gatatatatg aaaaagaaaa tgagtgtatt aattgttagt cttatgttga
     2401 ctcatgtaat gttggcaact gttcatattc attatctaca aaaatctgtc tatgaaactg
     2461 gtaggcaaca atggggtgat tggcaaaatg aagccaatgc attttgtatt gattcaacaa
     2521 ctctcagtat tgttaacctt tctactaagt cagaaacagt atatgttcgt caaggtgaag
     2581 caataggtgg atataatgaa cgaggaaact actatcgttt atttgaagct caaaatgaaa
     2641 aaggacagaa atgtctgttt tttttgtatt cagaccgaaa tgtaggctta ttattcaagt
     2701 gcgaagaagc aggtgtcatc atacaatatg cgggatatta gtgaatacaa tcaggtatta
     2761 tagttgtttg aagtatattt actacatatt tcttgttcct ttcattatac tattaaaaga
     2821 ggctggctca ttttattttg atccagcctc ttttaatatc tcatagggaa agttattttg
     2881 cacgttctac atacgatcct tctgttgtat ttatcttaat tttatcccca acattgataa
     2941 acaaaggaac acgaaccgta gcacctgttt ctactgtagc aggttttagt gtgttggttg
     3001 cagtatctcc ttttaatcct ggttcagtgt atgttacttc taggatggtg gtggtagcaa
     3061 ggtcggcaaa taatattgtt tctgtatcag catgtacagt tacttctgca atatctcctt
     3121 ctttcaagaa gtccactcca ttgattttgt ctttatcaat tgatatttgt tcaaatgttt
     3181 cgttgtgcat aaagttgtat cccatatcat ctttgtataa aaattgatat ggacgacgtt
     3241 cgatacgtac ttcatcaagt tttacgcctg cattaaatgt attttcgaga atacgacctg
     3301 tttgaacatt tttcattttt gtgcgtacaa atgctgcacc tttacctggt tttacgtgta
     3361 aaaattctac aattgtgtaa agtccattgt taaattcaat acacatacca tttcttatgt
     3421 ctgctgtagt tgccattgga ataatttaag tatataattt gctaataatt agggtgcaaa
     3481 aatagttatt tgttctataa catcaaaaaa aaaattatat caaacgagta tttttataag
     3541 ccttagctgt ttgatataaa ctgtaaatgg cttcagtcat gttgttccac ataaatttct
     3601 ttttctctat ttttacattg tttaccattt cttcttcgcg tttattgaca aaaaaatcta
     3661 ctaacgaatc cgcaatttct gtagtatttg gattaatcac atagcccact ttttgattag
     3721 gaatattaat gccacattga gcaatatttg taattagaat gggtttgtca aagtggtagg
     3781 ctacttgtaa aattccgctt tgatttactt ctctataggg ttgtccgaca atgctacatg
     3841 ctgagaaata gagactaaca acatgattag gaacatattc gtctacaaaa atgatattat
     3901 ttttgagtct attttcgtac atgatatcgg tataaaactc cttattattg ttaaaatctc
     3961 ccactaccaa aagctttata ggtaattgac gtacccgttc atctgcaaaa gcttcaataa
     4021 gtaaatccaa ccctttactt tctttgatag ctccaaaaaa taagacatat tgataatttg
     4081 gatttaaacc aagttgcaga agagctaatt ttttgtcagt acccttgcca aaaaggtcat
     4141 aaatagggtg aggagaaaaa atcactgggc gatgtttttt attaaaaggt ttaagttctt
     4201 cggttacttt atttgacata gaaataaacc catctaattg tttaaacatc atagtcgtag
     4261 ccattttttt tataccttca aatggatgat taatttctac gttgtgcatt acagctactt
     4321 gtatagccat tttctttaaa cgattggata tatagcttat acaaagagta gaagctatat
     4381 cagcgtagcc atatataatg agttcaaatt gctccttttt tatttctgca atgacttttt
     4441 cccattccaa tggattaaaa ttattgattt tttgtttaat
//
LOCUS       DAOSMY010000099         4451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19640_length_4451_cov_3.491583, whole genome shotgun sequence.
ACCESSION   DAOSMY010000099 DAOSMY010000000
VERSION     DAOSMY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19640_length_4451_cov_3.491583"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLD76_03280"
     CDS             <1..1107
                     /locus_tag="PLD76_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOU27059.1"
                     /translation="KLLVYKQGKIDQTVFSSISTHLPPRSLLVYNNTKVIHARLFFQK
                     DTGAIIEIFCLEPIQPSDYQMVFQSTKRCTWKCMVGNLKKWKQGALTLVCDVKGVSIE
                     LVAERDMINSQIHIHFSWNNVGVTFGDILEAIGKLPIPPYLHRESEENDEITYQTVYS
                     KEQGSVAAPTAGLHFTDEVLARLHQTGICTTEITLHVGAGTFLPVKSATIGEHTMHTE
                     MVVISKQTIKHLQASIGNIIAVGTTSVRSLESLYHIGVQVSKQQYHLSVSQWEPYSDH
                     QLLSPFEALQAVIQYLNNNGLTEIRFSTNIIILPSYQRKIVTGLITNFHQPKSTLLLL
                     IAAFVGEDWKKMYEYALNNDFRFLSYGDSSLLLP"
     gene            complement(1170..2015)
                     /locus_tag="PLD76_03285"
     CDS             complement(1170..2015)
                     /locus_tag="PLD76_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU27060.1"
                     /translation="MDFIKKRTLQSCIVILLIVTQSCNKLPEFDPLNFVKTNYEVDKR
                     FDDSQTLNDQSPLPDIQVMSETYRVVGASDLHITGNSEQVERFFSYTEEEDPSLVLIV
                     GDISNGLSHQMTSANNQFKAWNQPPSYFVAGNHDLFFSWSQYVQLFGSSTYTFTVHTP
                     TATDLYIGLESGSGTLGKKQTAWLVEQLQKRYEYRHCIVFTHTNFFARQFDTNGIYTK
                     EERMALLSLFAQYEVSMVISGHSHKENRTTFGNVHYITMPPLVDGGYMVFEVEESITT
                     RTMQL"
     gene            complement(2003..2686)
                     /locus_tag="PLD76_03290"
     CDS             complement(2003..2686)
                     /locus_tag="PLD76_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27061.1"
                     /translation="MPLKALIISLLCLCSYATAQTSLLHRIGIGHNPVKRTYAEISTV
                     GSYTSTCWELEAGLNWQGNTYYGSSIQAFTLGALYNLPVSQPISVGIHYMYHPHNQLL
                     LSEQNLALMATYMPQHLDVYIGSYIRHFTSKQFSNQSVSELGYLLYQLNVYVFPKNYI
                     YNAMIGVRSYDTFEIEQAGNPMYVLQLSYRPSTIHYFLEGCVKPAGFYNIHVNPFHTQ
                     LRGGILWTL"
     gene            complement(2677..3576)
                     /gene="miaA"
                     /locus_tag="PLD76_03295"
     CDS             complement(2677..3576)
                     /gene="miaA"
                     /locus_tag="PLD76_03295"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU27062.1"
                     /translation="MKHLVVLLGPTGVGKTHASIQLAQTYNAPILSADSRQLFKGIEI
                     GTAAATKEEKDLVPHYFVGTKELDETYSAGAYEEDVLALLPTLFQTHDIVLLVGGSMM
                     YIDAICKGMDDIPEVDQSIRQNVHKLYQENGLESVRELLLKLDPAYYSTVDLKNTQRV
                     MHAVEVSLMIGKPYSSLLTKKTANRNFTITKIGFNLPREELYQRINMRVEQMFEKGLV
                     EEAQNLYTKKELNSLNTVGYKEIFDYIDGIYPSIEIAKEKIKQHSRNYAKRQLTWFRK
                     DKSIVWFHPDDVDGCIKHINACL"
     gene            complement(3573..4136)
                     /locus_tag="PLD76_03300"
     CDS             complement(3573..4136)
                     /locus_tag="PLD76_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27063.1"
                     /translation="MLYKFIFISDEVDNFMREITIDSAATFLDLHKAILESVSYTEEG
                     MASFFICNDYWEKEQEITLIEMDTSSEYDNYVMEQTGLEEFISEEKQKILYVFDYFTD
                     RAFFIELKEIIPTKRQEKALCTKKMGEAPSQFVADIFEEDKVTKPAKVVTPLDENFYG
                     DEDFDMDELDEESFSDVDFSEESDPLK"
     gene            complement(4252..>4451)
                     /locus_tag="PLD76_03305"
     CDS             complement(4252..>4451)
                     /locus_tag="PLD76_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU27064.1"
                     /translation="VVQARHIAMYFSKQLTQESLTTIGNSIGKRNHATVIHACKTVKD
                     LMDTDKTFRGNVLEIEKKLTF"
BASE COUNT         1329 a          780 c          778 g         1564 t
ORIGIN      
        1 aaattattag tatacaaaca gggaaaaatt gatcaaacag tattttcttc tatctctaca
       61 catttacctc cacgtagttt gttggtatat aataatacaa aggtaattca tgctcgttta
      121 ttttttcaga aagatacggg agctatcatt gagatttttt gtttggagcc tatacaacct
      181 tctgattatc aaatggtttt tcaatcaaca aaacgttgta catggaaatg tatggtagga
      241 aacctaaaaa agtggaaaca aggagcttta accttggtat gtgatgtgaa aggtgtgtcc
      301 atagagctag ttgccgaacg tgatatgatt aattcgcaaa tacatataca tttttcgtgg
      361 aataatgttg gtgtaacatt tggtgatata ctcgaagcca taggaaaatt gcctatacct
      421 ccttatttgc atcgtgaatc ggaagaaaat gatgaaataa cgtatcaaac ggtatattcc
      481 aaagagcaag gttctgtggc tgcacctacg gctggtttgc attttacaga tgaggtacta
      541 gcacgcttgc atcaaacagg tatctgtact acagaaatta cgttgcatgt aggtgctggc
      601 acttttttac cagtcaagtc ggctactatt ggagaacata ccatgcatac tgaaatggtg
      661 gttattagca aacaaactat caaacacctc caagcatcta ttggtaatat tattgctgtt
      721 ggtacaacta gtgtccgttc gttggagtca ttgtatcata taggtgtgca agtatcaaaa
      781 caacagtatc atctatctgt ttctcaatgg gaaccttatt ctgatcatca gttactatct
      841 ccttttgagg ctttacaagc agtaatacag tatctgaata ataatggatt aacagaaatt
      901 cgtttttcaa cgaatattat tattttacct tcttaccaac gcaaaatagt tactggatta
      961 atcactaatt ttcatcagcc caaaagtact ctattgttgc ttattgccgc ttttgtgggt
     1021 gaggattgga aaaaaatgta tgaatatgca ctgaataatg attttcgttt tcttagttat
     1081 ggtgatagtt ctttactttt gccataatta atcctgatta tgcttcattt tttttgaatg
     1141 tataaaatga atacacgtat caatatggtc taaagttgca tggtgcgagt tgttattgac
     1201 tcttcaactt caaacaccat atatccacca tctaccaatg gaggcatagt aatatagtgc
     1261 acattgccaa atgttgtacg gttttcttta tgtgaatgac cgcttataac cattgatact
     1321 tcgtattgag caaataatga gagcaaagcc attcgttctt ctttggtgta tatgccgttt
     1381 gtgtcaaact gtcgtgcaaa aaagttggtg tgtgtaaaca caatacaatg acgatattca
     1441 taacgttttt gcagttgttc gacaagccag gctgtttgtt tttttcctaa agtaccactg
     1501 ccactttcca agccgatata caaatcagtg gctgtagggg tatgaaccgt aaaggtgtac
     1561 gtactgcttc caaaaagttg tacatattgg ctccagctaa aaaataaatc atggttgcct
     1621 gccacaaaat aagagggtgg ttgattccac gccttaaatt gattgttagc agatgtcatt
     1681 tgatgcgata atccattgga aatatctcca acgattaata ctagcgaagg atcttcttct
     1741 tcggtgtagg aaaagaacct ttctacttgc tctgaattgc ctgtaatatg taaatcggat
     1801 gctccgacta cacgatatgt ttcgctcata acctgaatgt ctggaagtgg cgattggtcg
     1861 ttgagagttt gtgagtcgtc aaatcgttta tcaacttcat aatttgtctt tacaaaattt
     1921 aaagggtcaa attcgggtag cttattacac gattgagtta ctataagcaa gataacgatg
     1981 caactttgta gggttcgttt ttttataaag tccatagtat acctcctctt agttgtgtgt
     2041 gaaatggatt tacatgaata ttataaaaac ctgctggttt aacgcaacct tctaagaagt
     2101 aatgaatagt actggggcga taactaagct gtagtacata cattggattg ccagcttgct
     2161 ctatctcaaa agtatcataa ctacgtaccc caatcatagc attgtatata taattttttg
     2221 gaaatacgta tacattcaat tggtaaagta aatatcctaa ttcactaact gactgattac
     2281 taaactgttt tgatgtgaaa tgtcgaatat aagagccaat gtacacatca agatgctgag
     2341 gcatatatgt tgccatcaaa gcaaggtttt gctcacttaa tagaagttga ttatggggat
     2401 gatacatgta atgtatacct actgaaatag gttgtgagac aggtaaatta tacaatgctc
     2461 ccaatgtaaa ggcttgtatg gaagaaccat aatatgtatt tccttgccaa ttgagtcccg
     2521 cttccaattc ccaacaggta cttgtatagc ttcctactgt gctaatttct gcatatgttc
     2581 tttttacagg gttatgtcca atacctatac gatgcagtaa gcttgtttga gctgtagcat
     2641 atgaacaaag gcataataaa ctgattataa gagctttcaa aggcatgcat tgatatgttt
     2701 gatacaacca tctacatcat ctggatgaaa ccatacaatg cttttatctt tccgaaacca
     2761 ggttaattgt cttttagcat agtttcttga gtgttgtttt attttttctt ttgctatctc
     2821 aatacttggg tatataccat caatataatc aaatatttct ttgtacccaa ctgtattcaa
     2881 agagtttaac tcttttttgg tatataggtt ttgtgcttct tctaccaatc ctttttcaaa
     2941 catttgttct actcgcatat tgattcgttg atacaactct tctctgggca agttaaatcc
     3001 aattttagta atagtgaagt ttctgtttgc agtctttttt gttagtagag acgagtaggg
     3061 tttgcctatc attaagctaa cttctacagc atgcattaca cgttgtgtat ttttcaaatc
     3121 tactgttgaa taatatgctg gatccagttt taataataat tcccgtacac tttccaaccc
     3181 attttcttgg tataatttgt gtacattttg acggattgat tgatccactt cgggtatatc
     3241 atccatgccc ttacatattg catcaatata catcatagat ccacccacaa gtaacacaat
     3301 atcatgcgtt tgaaataatg ttggtaataa agcgagtaca tcttcctcgt atgctccagc
     3361 actatatgtt tcgtctaatt ctttggttcc tacaaaataa tgcggtacca agtctttttc
     3421 ttctttggtg gcagccgctg tcccaatttc tatgccttta aataattgac gcgaatctgc
     3481 tgacagaata ggggcattgt atgtttgtgc caattggata ctggcatgtg tctttccaac
     3541 acctgtagga cccaaaagta caactaagtg tttcatttta gagggtcact ttcttctgaa
     3601 aaatcaacat cgctgaaact ttcttcatca agttcatcca tatcaaaatc ttcatcacca
     3661 tagaaatttt cgtcaagggg agtaacgact tttgctggtt ttgttacttt gtcttcttca
     3721 aatatgtcag ctacaaactg cgatggagct tctcccattt tttttgtaca tagtgccttt
     3781 tcttgtcttt tggtaggaat tatttctttt agttcaataa aaaaagctct gtctgtaaaa
     3841 taatcaaaca catacaaaat tttttgtttt tcttctgaaa taaattcttc caaacctgtt
     3901 tgttccatca cataattatc atattcagag ctggtatcca tttcaataag cgtaatttcc
     3961 tgttcttttt cccagtaatc attacaaata aagaaagaag ccatcccttc ttctgtgtac
     4021 gatactgatt ccaaaatagc tttgtgtaaa tctaagaaag tggctgccga atcaattgta
     4081 atttcacgca taaaattatc cacttcgtct gaaataaaaa taaatttgta tagcatacta
     4141 gtttaatttt gcatatatat aattataggc gtaaaaatac agcttttttt ctttttgaga
     4201 aacattgtca ataaaaaaag cgatcgtttt ggtcgctttt tgctgagatg cttaaaaagt
     4261 taattttttt tctatttcta acacatttcc tctaaaggtt ttgtctgtgt ccatgaggtc
     4321 ttttactgtt ttgcacgcat gaataactgt agcatgattg cgtttgccaa ttgaattacc
     4381 aattgtggtg agagattctt gagtaagttg tttagaaaaa tacatagcaa tgtgtcgtgc
     4441 ttgtaccact t
//
LOCUS       DAOSMY010000100         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19719_length_4438_cov_2.974675, whole genome shotgun sequence.
ACCESSION   DAOSMY010000100 DAOSMY010000000
VERSION     DAOSMY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19719_length_4438_cov_2.974675"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PLD76_03310"
     CDS             <1..1242
                     /locus_tag="PLD76_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27065.1"
                     /translation="VYKQTHCNYTVANIDNIHLVAQSGTVSPYSRYGYGNLRSGGFTV
                     SNSLGGVGYGLRSNSMINPQNPASYTAVDSLSFMFDFGASASFNQFTSGFSQQQKSTA
                     QFDYLALQIPIIKNMGMSLGLMPFSTVGYAYSFSETLQFNADELLTKHTFEGNGGISR
                     LYGGLAYAIMPQLSLGVNVNYLFGNIVHTRELTFPDNSTYSSSKEESNLYVSTFLWDA
                     GIQFIQPVGKKDVLVVGGTYTLKTPMNIRSEIVTTSTQTITDDTNYSFDYPQSIGVGV
                     SYKHGNSVVFAADYSYQSFADALFYSQRDTLLNARKLAIGCEYVPNSTSRSYVQTIRY
                     RLGANIAESYTKVNEKPYSEMGITLGFGLPLKNSKSILNLNLEYGSRGIINQNLVRED
                     YVKIGLGVSINENWFFKRKFE"
     gene            1295..2641
                     /locus_tag="PLD76_03315"
     CDS             1295..2641
                     /locus_tag="PLD76_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27066.1"
                     /translation="MKAKLIFIGLVVLSVFTVNAQKGVDTGTRYGLGEDSIRCLENIS
                     LYQNYYHIKDYNTSFQHWRVVYTECPASSRDLYRIGSILLVWQMQNAQDVDKQKDYFR
                     QLMENYDQRIKYFGNDARFPESWVRGRQAIDYINYCPEDPLREKALPWLKYSIDERQA
                     DADADVIDGYMRVLDGLYRSDNEKYRDMYMTEYLRVGKILDTRIARACKFVDNYKLAQ
                     NNANALFIASGIANCTTLESVFASKVQESKSDIESLNTIISLFQSAKCTESEVYFDAS
                     SFAHVINPTAQSAAGIAYQSVKKEDFLKAVDYFEEATKLEKVDSVKYDYQYAIAAIYA
                     QMKRYTQARTHALRAATYNPKKGEPYILLAMMYADSNIFPDDKILQKTVYWAAVDKLE
                     RSRSVDPSSRERAQQLINTYRQYFPTKEEIFFKPELRVGESFVVGGWINETVICRE"
     gene            2658..3206
                     /gene="lptC"
                     /locus_tag="PLD76_03320"
     CDS             2658..3206
                     /gene="lptC"
                     /locus_tag="PLD76_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOU27067.1"
                     /translation="MKAYSITVVILATVILFLEACTSSIQNDAPKVDRELMPVLKSYG
                     VSSVVSDSGVTRFRFNTPVWYVYDKAEEPYWDFPEGVHFEQFDTDLKVYAEVDADTAM
                     YYTNAQKWTLSGNVKAKNVAGEVFESERIYIDQKTEKIYTDRFIKITQQQRIITGIGF
                     QSNHTLTQYTITKPQGIFPIEQ"
     gene            3203..>4438
                     /locus_tag="PLD76_03325"
     CDS             3203..>4438
                     /locus_tag="PLD76_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU27068.1"
                     /translation="MTTTVFLIFISLLFSAFFSGMEIAFYVSNKLRFEINKQHSLLSS
                     SILSIFYANPSKYISIILVGNNIALVVYGIQMAKFLEPYLLLFLHNDLLIATLQTIFS
                     TIVILLTGEFLPKVLFRLNADLWLKVFAIPLFLCYLFLYPVATFVYQISIFLFKILRV
                     DVSNSAGGMTLGRADLDNFLQQNYENTTDKKHIENEVKLFQNALDFSKIKLRDCIIPR
                     TEILAVDFDTDIEELRVKFVESGYSRILVFKEHIDNMVGYIHSIELFKKPRKWQDQIR
                     VLPIVPETMAANKLMNYLLQKKKSMALIVDEFGGTSGMVTLEDIVEEIFGEIEDEHDT
                     QDYVAKKISENEYVFSGRLEIDYVQEQFDIQLPVSDEYLTIAGLILHYYQGFPKINEE
                     IIIGNYMCKVIKASANKIEL"
BASE COUNT         1487 a          642 c          842 g         1467 t
ORIGIN      
        1 gtttataaac aaacacattg caattatact gtcgctaaca ttgataatat acatttagta
       61 gcacaaagtg gtacggtttc gccctattca agatatggtt atggcaacct acgttcagga
      121 ggttttactg tgtctaattc actcggggga gttgggtatg ggcttcgttc caactctatg
      181 attaatccac aaaatcctgc atcatatacc gctgttgata gtctttcttt tatgtttgat
      241 ttcggtgcat ctgcttcttt taatcagttt actagtggct ttagtcaaca acaaaaatca
      301 acagctcaat ttgattattt agcattgcag attcccatta tcaaaaacat gggtatgagc
      361 cttggattga tgccgttttc tacggttggt tatgcatata gtttttcgga aaccttacag
      421 tttaatgctg acgaattatt gacaaagcat acgtttgaag gtaatggagg aatttcaagg
      481 ttgtatggag gattagcgta tgccataatg ccacaattat cattaggcgt aaatgtaaat
      541 tatctttttg ggaatatcgt acatactaga gaactgacat tcccagataa ttctacttac
      601 tcctcatcaa aagaagaatc taatttgtat gtaagtacct ttttgtggga tgcgggaata
      661 caatttatcc aaccggttgg caaaaaagat gtcttagttg ttggtggaac gtatacactc
      721 aaaacgccaa tgaatattcg tagtgaaatt gttaccacat ctacccagac cattaccgat
      781 gataccaact attcatttga ttatccacaa tcaatagggg taggtgtaag ctacaaacat
      841 gggaatagtg tagtatttgc tgctgattat tcttatcaat catttgctga cgcacttttt
      901 tatagccaga gagatacttt gctgaatgct aggaaattag ccattggttg tgagtatgtc
      961 ccaaatagta catctcgctc ttatgtgcaa actatacgtt atcgtttggg agcaaacata
     1021 gcagagtcct atacaaaagt aaatgaaaaa ccatattcag agatggggat tacgttagga
     1081 tttgggcttc ctcttaaaaa ttctaaatca attttaaatt tgaatttgga atatggtagc
     1141 agaggtataa ttaatcaaaa tttggtgcgt gaggattatg taaagattgg actgggagtg
     1201 tctataaatg aaaattggtt ttttaaaagg aaatttgaat agtagctaca ataaaaacag
     1261 taggtcagat tgaaaaaaaa ttaaaaataa aggtatgaaa gcaaagttaa tttttattgg
     1321 tttagtcgtg ttgagtgtat tcacggtaaa tgctcaaaaa ggtgttgata cgggaactcg
     1381 atatgggtta ggagaagata gtatcaggtg tttagaaaac atcagtttgt atcaaaatta
     1441 ctatcacatt aaagattata atacatcttt tcaacattgg agagttgttt atacagaatg
     1501 tccagcttct agtagagatt tatacagaat aggatctatt ttgttagtat ggcaaatgca
     1561 aaatgcacaa gatgtagata agcaaaaaga ttatttccgt caattgatgg agaattacga
     1621 tcaacgtatc aagtattttg gtaatgatgc acgtttccca gaatcttggg taagaggtcg
     1681 ccaagctatt gattatatta attattgccc tgaagatccc ttaagagaga aagcattacc
     1741 ttggttgaaa tattcaatag atgagcgtca agcagatgca gatgctgacg ttattgatgg
     1801 ttatatgaga gtgttagatg gattgtatcg ttctgataat gagaaataca gagacatgta
     1861 tatgacggag tacttgcgtg tcggaaaaat tttggatact cgtattgcta gagcttgtaa
     1921 atttgtagat aattacaaat tagcccaaaa taatgcaaat gctttattta tcgctagtgg
     1981 aattgcaaat tgtactacac tagagtctgt ttttgcatcc aaagttcaag agtcaaaatc
     2041 tgatatagaa tcactaaata ccattatatc gttatttcaa agtgcaaaat gtactgaatc
     2101 agaagtttat tttgacgcat catcctttgc tcatgttata aatcctacgg cacaatcagc
     2161 ggcaggtata gcatatcaat ctgtgaaaaa agaagacttc ttaaaagcag tagattattt
     2221 tgaggaagca accaaattag aaaaagtaga tagtgtaaaa tacgattatc aatatgctat
     2281 tgcagctatt tatgctcaaa tgaaacgtta tacacaagca cgtacacatg cattacgtgc
     2341 agctacctat aatcctaaaa aaggagaacc atacattctg cttgcaatga tgtatgctga
     2401 tagtaatata ttccctgatg ataaaatttt acaaaaaact gtctattggg cagctgttga
     2461 taaattagaa agatcacgtt cagtagatcc ctcatcaaga gagagagctc aacaattaat
     2521 aaatacatat cgacaatatt ttcctacaaa agaagaaatt ttctttaagc cagaattgcg
     2581 cgttggagag tcttttgtag ttggaggatg gattaatgaa acggttattt gtagagaata
     2641 atttcaatat aaaacatatg aaagcatata gcataacggt tgttattttg gcaaccgtta
     2701 tccttttttt agaagcttgt acctcttcca ttcaaaatga tgcccctaag gtagatcgag
     2761 agttgatgcc tgtattgaaa tcgtatggtg tgtcttcagt cgtatctgat tccggcgtta
     2821 cacgttttcg gttcaatacg ccagtatggt atgtatacga taaggcggaa gagccttatt
     2881 gggattttcc agaaggagtt catttcgagc agtttgatac cgatttaaaa gtgtatgctg
     2941 aagtagatgc cgatactgct atgtattata ccaacgcaca aaagtggaca ctttctggta
     3001 atgtaaaagc aaaaaacgtt gcgggagaag tatttgaatc tgagcgtatt tatattgacc
     3061 aaaaaactga aaaaatttat acagataggt tcattaaaat aactcaacaa caacgtatta
     3121 ttacaggcat aggttttcaa tctaaccata ctcttacaca gtatacaata acaaaacctc
     3181 aaggtatatt tccgatagaa caatgacaac aactgttttt ttgatattta tatctctgct
     3241 tttctctgca tttttttcag gaatggagat tgctttttat gtttcaaata aactgcgttt
     3301 tgaaataaat aaacaacaca gcttactatc ttcgtctatt ctttcaattt tttatgccaa
     3361 tcctagtaaa tatatttcaa tcattttagt tggtaataat attgctcttg tagtatatgg
     3421 tattcagatg gcaaaatttt tagagccata tttattgtta ttcttacata atgacttgtt
     3481 gatagctact ttacaaacaa ttttttcaac aattgtgatt cttttaactg gagaattttt
     3541 gccaaaagtg ttatttaggc ttaatgcgga cctttggtta aaggtttttg ctatcccgtt
     3601 gtttttatgt tatttatttt tatatcctgt agctacgttt gtatatcaga tatcaatttt
     3661 tttattcaaa attctgagag tagatgtttc taattcagca ggggggatga ctcttggacg
     3721 agcagattta gataactttt tacaacaaaa ttatgaaaat acaactgaca aaaaacacat
     3781 agaaaatgaa gtcaaattgt ttcaaaatgc cttagatttt tctaaaataa agttgcgaga
     3841 ttgcattatt cctcgtacag aaatattagc ggtcgatttt gatactgata ttgaagaatt
     3901 acgtgtaaaa tttgttgagt cgggttattc gcgtatattg gtgtttaaag aacatataga
     3961 taatatggtt ggttatattc attcaattga attatttaaa aaaccacgaa aatggcaaga
     4021 tcagatacgc gtgttgccaa ttgtgcccga aacaatggcg gctaataagt tgatgaatta
     4081 tttgttgcag aaaaagaaaa gtatggcatt gattgtggac gaatttggcg gaacttctgg
     4141 gatggtaact ttagaggata ttgtagaaga aatatttggt gaaattgaag atgaacacga
     4201 tactcaagat tacgtagcaa aaaaaatcag tgaaaatgaa tatgtgtttt ctggaagatt
     4261 agaaatagat tatgtacaag aacagtttga tattcaactc cctgtttcag atgagtattt
     4321 aacaatcgcg gggcttatac tccattatta ccaagggttt cctaagataa atgaagaaat
     4381 aattattggt aactatatgt gtaaagttat taaggcttca gctaataaaa tagaatta
//
LOCUS       DAOSMY010000101         4428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19770_length_4428_cov_4.020581, whole genome shotgun sequence.
ACCESSION   DAOSMY010000101 DAOSMY010000000
VERSION     DAOSMY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19770_length_4428_cov_4.020581"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLD76_03330"
     CDS             complement(<1..918)
                     /locus_tag="PLD76_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU27069.1"
                     /translation="MNKLAHCIFLAIFVTHTLYSCTTNEPTSPPQPKEEHITMNINKS
                     SPKAFYYSIDDALENAETPEKKLKLGDSIYLLAKEKQWIPIVFADTAVFLYRHSSTNN
                     ATVEVFGDLNGWSASESPQISLSRYADTRLYAGIYKAPSTTTRVDYKLVVNNAWILDP
                     GNPKLAWGGMGSNSEVALSEYEYSPWVKARSDINKGTLSANQLITSSALGYNINYRVY
                     LPYGYDSELSYPVLFVTDGHEYINNNLGALPNVADNMLHENKIEPLIIVFVDPRDPTN
                     GTNKRMTEYVANDNFVTFLADELYTTIAET"
     gene            1105..1800
                     /locus_tag="PLD76_03335"
     CDS             1105..1800
                     /locus_tag="PLD76_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU27070.1"
                     /translation="MRKTHLLIGLLSIAIIFSGCKSWSNTAKGGLIGGGAGAAIGAGV
                     GILIGKNEKSAAIGAAVGTAVGSTTGLIIGKQMDKAAEKAAAIEGAKVDTITDANNLK
                     AVKVTFESGILFASGKSILSTKSKTSLSDFAAILVENPTMDVAIMGHTDNTGSLELNK
                     KLSQERAQAVADFLVSKNVPTKQIKEVVGKDFSEPVADNATVEGKAANRRVEIYMYAS
                     EEMIKNAENAVKK"
     gene            complement(1955..2650)
                     /locus_tag="PLD76_03340"
     CDS             complement(1955..2650)
                     /locus_tag="PLD76_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU27071.1"
                     /translation="MKKIRCLLVDDEHLALEILELYCSKIDYLDVVGTCKNADEAAIF
                     LQHNKVDLLLLDIQMPRLNGMEWYKNLSSKPLVIFCTAFQHFAVESYEVNAIDYLLKP
                     FSLERFNKAIEKTKEIIEKKTNQETIEQSIFVRSDRKEIRVKIDDILFIQSMSNYYVV
                     TTTEKKIIGYGSISTLESTLQAFNFIRIHRSYLVAKNKIGSIATNSIIVSTYELPIGR
                     NYRKNIENIAKLS"
     gene            complement(2647..3630)
                     /locus_tag="PLD76_03345"
     CDS             complement(2647..3630)
                     /locus_tag="PLD76_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU27072.1"
                     /translation="MVVLLFTFFWGTRYGNYWVCFYNELYFLPIKIAATYFGLYYLIP
                     QLFKRNYYRVVVAGSFMLIGSGLLQRIIGYYSFIPEVGFYLPDIPLFDITEILHKIMD
                     INAVMIIPFSIKFYTHYLDKEHKILILSQEKTEAELQFLRSQIQPHFLFNVLNDIYAM
                     ALLNKKETPDSILKLADLMRYVLQESTASKVPLEKEINYIRNYIDLEKLRYNDLYAIS
                     FEIHGDSANKMITPLLLLPFIENAYKHSTTNDTGGAWIKIRISLTNEVLTLEIMNSFD
                     RNIPKVTQVSSGIGIQNVKKRLDILYQGKYTFYTENTPESYKTILTINLDT"
     gene            4002..>4428
                     /locus_tag="PLD76_03350"
     CDS             4002..>4428
                     /locus_tag="PLD76_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOU27073.1"
                     /translation="MSQNNAFVGKKIKTIREGKDITVEQLAERSGLAVEQIRSIEEDE
                     KLPSLAPLTKIARALGVRLGTFLDDNTEIGPVVCRKGEKSGSLSFSNDMVGARKHMEY
                     YSLAGQKAGRHMEPFIIDIQPSVSKDFILSAHEGEEFIYV"
BASE COUNT         1443 a          673 c          828 g         1484 t
ORIGIN      
        1 tgtttcagca atggtagtat ataattcatc tgcaaggaat gttacaaaat tatcattggc
       61 aacatattca gtcattcgtt tgttcgtgcc gttggttggg tcgcgtggat caacaaatac
      121 aataataaga ggttctattt tattttcgtg gagcatattg tcagctacat taggtaatgc
      181 acccaaatta ttgtttatgt attcatgtcc gtctgtaaca aaaagaacgg ggtaagacaa
      241 ctccgaatcg tatccataag gcaaatatac acggtagttg atgttgtacc ccaaagctga
      301 actggtaatc agttggttag cagaaagagt tcctttgttt atatccgaac gtgcttttac
      361 ccaaggagaa tattcgtatt cgctaagggc tacttctgaa ttggaaccca ttccgcccca
      421 agctaattta gggttgcctg ggtctagtat ccatgcgttg tttacaacta gtttataatc
      481 aacgcgggta gtagtagagg gggctttata aatgccagcg tataggcgag tatcagcata
      541 gcgagaaagt gatatttgag gggattcaga ggcactccac ccatttaaat cgccaaaaac
      601 ttccactgtg gcgttgtttg tactcgaatg gcgatacaaa aacacagccg tatcagcaaa
      661 cacaatcggt atccattgtt tttctttggc aagcaagtaa atgctatcgc caagtttcag
      721 tttcttttcg ggtgtttcag cgttttctaa agcatcgtcg atagaataat aaaatgcttt
      781 tgggctagac ttattgatat tcatggttat atgttcctct tttggttgag ggggagatgt
      841 tggttcgttt gtagtacacg aatacagggt atgagtaaca aaaatggcaa gaaaaataca
      901 atgagctaat ttgttcatag tacacttaat ttaagtgtta gtttatgttt caaaaatacg
      961 gaatatattt tgataaacca aatatattca taaaagtatc tttttaagat tttttgcaac
     1021 acagaatttt ttagatatag ctactctttg tatctttgtg gagattttaa taaaacaagt
     1081 aatttacaaa aaacgtttaa caaaatgaga aaaacacatt tacttattgg gcttttgagt
     1141 attgctatca ttttttcggg atgcaaatcg tggagcaata ccgctaaagg cggattaatt
     1201 ggaggaggtg ctggtgctgc aataggtgca ggagttggta ttttgattgg taaaaatgag
     1261 aaaagtgcag caattggtgc agcagtaggt acagcagtag gatccactac agggcttatt
     1321 attgggaaac aaatggacaa agctgcagaa aaagccgcag ctattgaggg tgctaaagta
     1381 gacacgatta ccgatgctaa taacttaaaa gcagtaaaag ttacgtttga atcgggtatt
     1441 ttgtttgcta gtggcaaaag tattcttagt actaaatcaa aaacctcatt gagtgatttt
     1501 gctgctattt tagtagaaaa tcctactatg gatgtagcta ttatgggaca tacagacaat
     1561 actggttcat tggagttgaa taaaaaatta tcgcaagaaa gagcacaagc tgtagctgat
     1621 tttttggtgt caaaaaatgt tccaacaaaa caaatcaaag aagtagttgg taaagatttt
     1681 tctgagccag tagctgataa tgctactgta gaaggtaaag cagcgaatcg tcgtgtagaa
     1741 atctatatgt atgcgagcga agagatgatt aaaaatgcag aaaatgcagt aaaaaaataa
     1801 cattcttttg tttagggaga aagagtttgc tacatagcaa actctttttt tgtattagta
     1861 taagtagttt cagtttacag tgcgttgtaa aatagaatac tggtaagggt ctaaagataa
     1921 gtttgtttcg agttctatat tttgttttgc ataattatga taatttagca atattttcta
     1981 tattttttcg ataattacgg ccaattggca gttcgtaagt agatacaata atagagtttg
     2041 tagcaatgct tccaatttta tttttagcta ctaaatatga ccgatgaatg cgtataaaat
     2101 taaaagcttg aagagttgac tccagtgttg aaatagaacc gtatccaatt atttttttct
     2161 ctgtcgtggt tactacatag tagttgctca tactttgaat aaatagtata tcgtcaattt
     2221 taacccgaat ttcttttctg tcagaacgaa caaaaataga ctgttctatg gtttcttgat
     2281 tcgttttttt ctcaattatt tcctttgtct tttctattgc tttgttaaag cgttccaatg
     2341 aaaatggttt aagtaaataa tcaatagcat ttacttcgta actttctacg gcaaaatgct
     2401 gaaaggctgt acaaaatata acaagaggtt tgcttgatag atttttatac cactccattc
     2461 cgtttagtct tggcatttga atatctaata atagcaaatc aaccttattg tgttgcaaaa
     2521 aaattgcggc ttcatcggca tttttacacg tgccaactac atcaagataa tcaatcttac
     2581 tgcaatatag ctccagtatc tctaaagcca aatgctcatc atctaccaat aaacaacgaa
     2641 tctttttcat gtatctaggt ttattgttag tatagtttta taactctcag gagtgttttc
     2701 tgtgtaaaaa gtgtacttac cctgatataa aatatccaat cgttttttta cattttgaat
     2761 gccaatacca gacgaaactt gcgtaacttt cgggatattg cggtcaaaac tgttcataat
     2821 ttcaagcgtt aatacctcat ttgttaggct gatacgaatc tttatccaag ccccacctgt
     2881 atcattagta gtagagtgtt tataagcatt ttcgataaat ggcagtagca aaagaggggt
     2941 aatcatctta ttcgcactat ctccgtgtat ttcaaaggaa atagcataca aatcattata
     3001 gcgtaatttt tctaaatcaa tgtaatttcg gatatagtta atttcttttt ctaatggcac
     3061 tttactagcc gtggattctt gcaaaacata ccgcatcaaa tccgctaatt ttaggatgga
     3121 atcgggtgtt tcttttttat tcaataatgc cattgcataa atatcgttaa gcacattgaa
     3181 taaaaaatgg ggctgtattt ggctccgtaa aaattgtagc tctgcttccg ttttttcctg
     3241 agataaaata agtattttat gctctttatc taaataatgg gtgtagaatt tgatggaaaa
     3301 aggaataatc attaccgcat ttatatccat tatcttatgt aaaatttcag taatgtcaaa
     3361 cagaggaatg tcgggtaaat aaaatcctac ttcggggata aatgagtagt aaccaataat
     3421 tcgttgtaaa agaccactgc caataagcat aaaactacca gcaactacaa cgcggtaata
     3481 gttgcgttta aaaagttgtg gtataagata atataatcca aagtaagtgg ctgcaatttt
     3541 gataggtaaa aaatacaact cgttgtagaa gcacacccaa tagtttccat aacgagtgcc
     3601 ccagaaaaat gtaaataata acaccaccat tacccaatat gtaatgtgca gtagccatcg
     3661 attttttcca gttagcagtc gcatgtaagt aattgattta gttgaacaaa tatacagttt
     3721 ttttgcaaat tgtactacct ctctctattt ttctttttat atttatgcaa atagaagttc
     3781 tttttatgta aacgtatttt tctctgttag ttagaagttt tagaatatgt cttctggact
     3841 attttcgttt ttatttggat gtgaaaaaat acttgattaa cgcaaaaata taacttttgg
     3901 ttgtaaataa gctaaaaata ttgtttgata agataaaatg tatatttttg tggtcgatat
     3961 actataatat tttaatctga taaccataaa aaattaaatc tatgtcacaa aacaatgcat
     4021 ttgttggtaa aaaaattaaa acaattagag aaggtaaaga tattaccgta gaacaattag
     4081 ccgaacgttc gggtttagct gtagagcaaa ttcgttcaat agaagaagac gaaaaacttc
     4141 cctcattagc tccattgacc aagatagcac gtgcattagg tgttcgctta ggtacttttt
     4201 tggatgataa tacggaaatt ggtccagttg tatgtcgtaa aggtgaaaaa tctggaagtt
     4261 taagtttttc aaacgatatg gtaggtgccc gcaagcatat ggaatactat tctttggcag
     4321 gtcaaaaagc tggtcgtcat atggaacctt ttattataga tatccaacca tctgtaagca
     4381 aagattttat tttatcagca cacgaagggg aagagtttat ttatgttt
//
LOCUS       DAOSMY010000102         4402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19915_length_4402_cov_3.247987, whole genome shotgun sequence.
ACCESSION   DAOSMY010000102 DAOSMY010000000
VERSION     DAOSMY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19915_length_4402_cov_3.247987"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PLD76_03355"
     CDS             complement(<1..158)
                     /locus_tag="PLD76_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOU27074.1"
                     /translation="MEELTLTTPSLLFSAVSLLLLAYTNRFLSYAQVVRTLKENYTKD
                     HSALTGAQ"
     gene            complement(265..1344)
                     /locus_tag="PLD76_03360"
     CDS             complement(265..1344)
                     /locus_tag="PLD76_03360"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOU27075.1"
                     /translation="MKNLHIGGLVIPVPIIQGGMGVGISLSGLASAVANQGGVGVMSA
                     AGLGLLYRHLSSDYPEACILGLREEIKKAREKTKGIIGVNIMVAMTNFADMVKTSIAE
                     KVDIIFSGAGLPLDLPSFLQKDSTTKLVPIVSSARAAKLICDKWKAAYNYLPDAVVVE
                     GPKAGGHLGYKESQLSDDAYRLETIIPEVVATIKEIETTHDCKISVIAAGGIYTGADM
                     YDIMQLGADGVQLGTRFVTTTECDASEIFKQAYIDATEADVEIIKSPVGMPGRAIFSD
                     FLQKVKDGEKQPKSCPFHCIKTCQIETSPYCIMLALFNAYKGNMTMGYAFAGTNAHLA
                     TAIVTVKDTMDAIQEEYKAREKELV"
     gene            complement(1495..3633)
                     /locus_tag="PLD76_03365"
     CDS             complement(1495..3633)
                     /locus_tag="PLD76_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU27076.1"
                     /translation="METNTELELAWRCVVQTGTHLFLTGKAGTGKTTFLHQLKTTSPK
                     RMVVLAPTGIAAINAGGVTIHSFFQLPFAPYIPGTEFATDTKYQFNKEKINIIRSLDL
                     LVIDEVSMVRADLLDSVDAILRRYRRTTVPFGGVQLLLIGDLHQLSPVIKEEERRLLE
                     AYYPSSYFFNSKALQKTAYLTIELKQVYRQSEKHFLDLLNQIRDNKATSTTLEELNKR
                     YIPDFIPPAGEEYIRLTTHNYQAQTINDQHLAKISSPAFSYKAQVNGNFPQHAYPADE
                     LLTIKEGAQIMFIKNDASFPKRFYNGKIGKVTHIDEHVIEVQTNDDPEPFSLQKAVWT
                     NAKYILNKETNEISEDIEGTFEQYPIRLAWAITIHKSQGLTFDKAIIDIASSFAHGQT
                     YVALSRCRTLNGMVLNSPISHRAIIKDAVIHDFVQQSATDAPDESKIAVLERAYFSYL
                     VTEMVQFSDITKLLVRGSYLFKESPLIGMYPSIHEQLKQSIEHIQQDIEQVSSRFLSV
                     FFSMFRNTPDYQDNAALQERIQSAASYFLVQLNTLVAPLIAAIKVDVDNKETEKQVND
                     LHAQLSETLQIHTACLSSCKNNGFSVSAYQKAKLEAAIEHTSTKKRTAKASEKQKVSI
                     NSGETEHAQLYEHLRAWRLQEASERKLPAYAILHQKALLGIVQHLPLSPTELLQIPHL
                     GKRTVTKFGDAIIEIVREFVQSKENDKTNS"
     gene            3791..4261
                     /locus_tag="PLD76_03370"
     CDS             3791..4261
                     /locus_tag="PLD76_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOU27077.1"
                     /translation="MESTSFMQLAIDLAIENVKQGKGGPFGAVVVKEGKIVGKAANSV
                     TSTNDPTAHAEVNAIRQACAHLKTFDLSGCELYASCEPCPMCLAAIYWARIDKVYVSA
                     TKHDAAEVGFDDAFIYEEFALPKENRKVLQVPLLVQEGKAPFKAWKQKTDKIPY"
BASE COUNT         1238 a          798 c          865 g         1501 t
ORIGIN      
        1 atttgtgccc ccgtaagggc tgaatgatct ttggtgtagt tttctttcag cgttcgtacc
       61 acttgggcat acgacaaaaa acgattggta tatgccaaca gcaataacga tactgcagaa
      121 aaaagcaatg aaggtgtagt taatgtaagt tcttccatat ttacgttata gtaggtttaa
      181 cgaaatgaat ccacttttac cacactagtt gtttttgtta actgttgtgg tagtagtatt
      241 tgcttacaaa aatacaattt tttattaaac tagctctttt tctctagctt tgtattcttc
      301 ttggatagca tccatggtat cttttacggt aacaattgct gtagccaagt gtgcattcgt
      361 gccagcaaat gcgtaaccca tggtcatatt gcccttgtat gcattaaaca gagccaacat
      421 aatacaataa gggcttgttt ctatttggca ggttttgata caatggaaag gacaactttt
      481 cggctgtttt tcgccatctt ttactttttg taaaaaatcg ctaaagatcg ctctaccagg
      541 cattcccact ggacttttta ttatctctac atccgcttcg gtagcatcaa tatacgcttg
      601 tttaaaaatt tctgaagcat cacattcggt cgtggttaca aaacgtgttc ctagttgaac
      661 cccatcagca cctaattgca tgatgtcata catgtctgca cctgtgtaaa taccgccagc
      721 agcaataaca gaaattttgc agtcgtgtgt tgtttctatt tctttaattg tagctactac
      781 ttctggaata atagtctcca atctgtaagc atcgtcagaa agttgagatt ctttatagcc
      841 taaatgtccc cctgcttttg gaccttctac caccacagca tctggcaaat agttgtatgc
      901 agctttccat ttgtcgcaaa ttaatttggc tgctctagct gacgaaacga tgggaactaa
      961 ttttgtggtg ctatcttttt gtaaaaatga aggtaaatcg aggggtaatc ctgcaccaga
     1021 gaaaatgata tctacttttt cggctattga tgttttaacc atatccgcaa aattggtcat
     1081 ggctaccatg atatttaccc caataattcc ttttgttttt tctcgtgctt tttttatctc
     1141 ttcacgcaat cctaatatac atgcttcggg ataatctgat gataaatgtc ggtataacaa
     1201 tcctagtcct gcagcagaca taacgcctac tcctccttga ttagctactg ctgaggctaa
     1261 gcctgataat gaaataccaa cgcccatacc tccttgtata attggaacgg gaataactaa
     1321 gcctccaata tgtaaattct tcatatagta tatataaaat ggtgtgcaaa ggtagtgtta
     1381 atttgcggta tattacgcat gcggatttaa ttggcgtata ttcggcagaa aaaagccaca
     1441 aataattgca cagataattt ttgaacgaaa aaattaattt acgtatatgt ttggttatga
     1501 gttagttttg tcgttttctt ttgattgcac aaattcgcgt acaatttcaa ttatcgcatc
     1561 gccaaatttt gtaacagtac gtttgccaag gtgaggaatt tgaagtaatt ctgttggcga
     1621 caagggtaaa tgttgcacaa tacccaacag agctttttgg tgtaaaatgg cataggcagg
     1681 tagctttctt tcagaagctt cttgtagtcg ccaagctcgt aagtgctcgt atagttgtgc
     1741 atgttcagtt tctccactat tgatggatac tttctgtttc tcagatgctt tggcagtacg
     1801 tttttttgta gatgtatgtt caatggcagc ttctaatttg gctttttgat aggcactaac
     1861 gctaaaccca ttgtttttac aactgcttaa acaagcggta tgaatttgta gagtttcact
     1921 aagttgagca tgtaaatcgt ttacttgttt ttctgtctct ttgttatcta catctacttt
     1981 tatcgcagca ataagtggtg ctaccaatgt gttgagttga accaaaaaat aacttgctgc
     2041 cgactgtatg cgttcttgta atgctgcatt gtcttgatag tcgggtgtgt tgcgaaacat
     2101 cgaaaagaaa acagaaagaa aacgactact tacttgttca atatcttgtt gtatgtgctc
     2161 aatggattgt tttagttgtt catgaattga aggatacata ccgatgagtg gagattcttt
     2221 aaataagtaa ctaccacgca caagaagttt ggtgatgtca gaaaattgca ccatttctgt
     2281 tactaaatat gaaaaatacg ctcgttctaa tacagctatt ttactctcat ctggagcatc
     2341 tgtggctgat tgttgcacaa aatcatgtat tacagcgtct tttattatgg ctcgatgcga
     2401 aataggagaa ttaagcacca ttccatttaa tgtgcgacaa cgactaagtg ccacgtatgt
     2461 ttgtccgtga gcaaacgagg aagcaatatc aataatcgct ttatcaaaag ttaatccttg
     2521 gcttttgtga atcgtgattg cccaagccaa gcgaatagga tattgctcaa aagtgccttc
     2581 tatatcttcg ctaatctcat tagtttcttt gttcaatatg tattttgcat ttgtccatac
     2641 ggctttttgc aaactaaatg gttcggggtc atcgtttgtt tgcacttcga taacatgctc
     2701 atcgatatgt gttaccttgc ctatttttcc gttgtaaaag cgttttggaa agcttgcatc
     2761 gtttttgata aacataattt gagctccctc tttgatagtc aacaactcgt cagcaggata
     2821 agcatgttgc gggaaatttc catttacttg tgctttatac gaaaatgcag gactgctaat
     2881 ttttgctaaa tgttggtcgt tgattgtttg tgcttggtaa ttgtgtgtag ttaagcgaat
     2941 atactcttcg cctgctggag gaataaaatc tggaatatag cgtttgttta attcttcaag
     3001 cgtcgtggag gtagctttgt tgtcgcgtat ttggtttaat aaatccaaaa aatgtttctc
     3061 gctttggcga tatacctgtt ttagttcgat ggtaagataa gctgtttttt gcaaggcttt
     3121 gctgttaaaa aaatagctac tggggtaata cgcttctagc aatcttcttt cttcttcttt
     3181 tattacagga gatagctggt gcaaatcgcc aataagtaac agttgtacgc ctccaaaagg
     3241 cacggttgtg cgtctgtatc ttctcaatat ggcatctaca ctgtccaaca aatcggcacg
     3301 taccatgctc acctcatcta ttaccaataa atctaaactg cgtatgatgt ttattttttc
     3361 tttgttaaat tggtattttg tatctgtggc aaattctgta ccaggaatat agggagcaaa
     3421 aggtagttga aaaaatgaat ggatagtaac gcctcctgca tttattgccg caatgccggt
     3481 aggagcaagt actaccatgc gtttgggcga ggttgtttta agttgatgca agaaagtggt
     3541 ttttcctgta cccgctttac ctgttaaaaa caagtgagtg cctgtttgca ctacacatcg
     3601 ccacgctaat tcaagttctg tatttgtttc cattcgtatg caaaaatacg atatatcttt
     3661 ggtaaacgag ccatatttta tactgcttct gtatttttta ttacacaagt gtgttatcat
     3721 tcgctagtat tttgtatctt tacacgtaag agatagttga acatagaggt tttacgaata
     3781 aagaatgaag atggaaagta ctagttttat gcaacttgct atagatttag cgatagagaa
     3841 tgtaaaacag ggcaaaggcg gaccctttgg tgccgtagta gtaaaagagg gtaaaattgt
     3901 aggtaaagca gccaattctg ttacttcgac caacgatcct actgctcatg ccgaagtaaa
     3961 tgcaattagg caggcttgtg ctcatttaaa aacgttcgat ttatcaggct gtgagttgta
     4021 tgccagttgc gaaccttgcc ctatgtgttt ggctgccatt tattgggcac gtatcgacaa
     4081 agtatatgtt tcggctacta aacacgatgc tgcggaggtt ggttttgatg atgcttttat
     4141 ttacgaagaa tttgccttgc ccaaagaaaa cagaaaagta ctacaagtac ctctattggt
     4201 acaagaaggc aaagctccat ttaaggcatg gaaacagaaa acagacaaaa ttccgtatta
     4261 acaaacctag attatttctt gtagacataa aaaataggct gactcaacat aaaatgggtc
     4321 agcctattac acttttttta agggtatggc ttattttgta aatacttttt cgtttggttg
     4381 cacatcttcg gtaatccatg ta
//
LOCUS       DAOSMY010000103         4400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19927_length_4400_cov_2.987342, whole genome shotgun sequence.
ACCESSION   DAOSMY010000103 DAOSMY010000000
VERSION     DAOSMY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19927_length_4400_cov_2.987342"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLD76_03375"
     CDS             complement(<1..1104)
                     /locus_tag="PLD76_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Por secretion system protein"
                     /protein_id="HOU27078.1"
                     /translation="MKKSFFLFFLFLFSLSFFAQQLVTTTPAFIAQNYAGEIKVVFDA
                     TQGNAAMLGSATCYAHAGVILTSGPGWKKAPVWLDNASKYQLVSIGTDLWELTIPNLS
                     SYYNLAEGEVIAQLAFVFRNADGTKQGKTAAGGDIFIDLYDPSVLSMSVTNPSTDAVI
                     NKDASLVVQANVSMSSKIELFINSQLVKTEETATSISYSFDTSVAGDYTVKIKATNGE
                     NSVEMSREICVKSPVQYAPVPTGMLEGINYDAQNNSVTLVLRAPLKENVFVIGDFNEW
                     KIKNQYQMFRNEENGILWWLTIDNLNPDTEYAFQYLIDGNLKVSDAYTEKVIDPWNDK
                     WIPTSVHPRLTYPEKADGLVSVFQINRPQYQWEV"
     gene            complement(1352..4387)
                     /gene="secDF"
                     /locus_tag="PLD76_03380"
     CDS             complement(1352..4387)
                     /gene="secDF"
                     /locus_tag="PLD76_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOU27079.1"
                     /translation="MQNKGLVLSFTIALVLVCLFYLSFNVVTSHYDKQAKEFAQGNVM
                     EEYRYLDSLSTEKVWLNYTLKECREKELNLGLDLKGGMNVIIEVSVPDILKTLAGNST
                     DETFTKAIQLAHQRQATSQKDFIDLFQEAFQEIDKDARLAAIFSTYELKDKVNLKSTN
                     AEVIAVIKKEVQATVDNSFNVLRTRIDRFGVVQPNIQRLETNGRILIELPGVKEPERV
                     RKLLQGSANLEFWETYTLPEVYESLVTANNVIKESQKDKIPVVETPTSVADSTSEEKV
                     EEQKDNVNSLLSQLEQPKDTLSEDDFSKENPLFSVLQLNVQNGQLVPTPAVGVAHQKD
                     TALVKSYLNLRQVKEVLPRNVEFKWSVKSIDTKGNFFELIAIKVTSRDGKAPLEGSAI
                     SDARSDFGQFSSNSVVSMTMNPEGARTWARLTKENIGRSIAIVLDDMVYSYPNVNTEI
                     TGGRSEISGNFTVEEAKDLANVLKSGRMPAPSRIVQEDVVGPSLGQEAIQKGLLSFAI
                     AFIGVMLYMILMYGVVPGLVVDGALLFNVFFLVGILASFKAVLTLPGIAGIVLTLGMA
                     VDANVLIYERIKEELAAGKTHKSAIADGYKNALSAIFDSNITTIITAVILFYFGTGPI
                     KGFATTLIIGIVTSFFTAVFVTRWAYETLLDKNFTEKLSFTTKVSANFLSNVNLDWVK
                     LRKVGYILSITFMVVAIFSLAFHGLNQGIDFTGGRNYVIKFEESVSTQDLQRKVAPGF
                     EDASVSVITIGAVSENQVRISTNYKIQDNSDTVDDEIENVLYEQLKPFIKNQTVTRDM
                     FVKGYVINEDGDAQLSSAGSVRPLGVQSSQKVGPAIADDIKNAAYLAIFFSLIGIGLY
                     ILLRFKDISYSASIVVSLIHTSVFVLGVYSLFYSVMPFSMEIGQSFIAAILTVIGYSV
                     NDTVVVFDRLRETIGLYPKRDRKEMFNYSVNATLSRTFNTTFSTLLVLIIIFLLGGDT
                     IRGFVFALLLGITIGTYSTLFVSVPLAYDIQTKKLNKKK"
BASE COUNT         1447 a          884 c          681 g         1388 t
ORIGIN      
        1 aacttcccat tggtattgtg gtctatttat ttgaaacaca gaaaccaacc catcagcctt
       61 ctctgggtac gttaatcgtg gatgaactga tgttggtatc cacttatcat tccatgggtc
      121 aattactttt tcagtatacg catcactcac ttttagatta ccatctatta aatactgaaa
      181 cgcatattcg gtatctggat taaggttatc aatggttaac caccacaaaa tgccattttc
      241 ttcattgcga aacatttggt attggttctt tattttccat tcgttaaaat cacctataac
      301 aaacacattt tcttttaatg gggctctaag tactaaagta acactattat tttgagcatc
      361 ataattaatc ccttctagca tacctgttgg tacaggtgca tattgtacag gagatttcac
      421 acaaatctct ctactcattt caacactgtt ctctccgttt gttgctttaa ttttcacagt
      481 ataatcacca gcaacacttg tatcaaagct ataagaaata gaagtagcgg tttcctctgt
      541 ttttactaac tgactgttta taaatagctc tattttagaa gacattgata catttgcttg
      601 cacaaccaaa gacgcatcct tatttattac agcatctgtc gaagggttcg taacgctcat
      661 actcaacaca gaaggatcgt ataagtcaat aaagatatcg ccaccagcag ctgtcttgcc
      721 ttgcttggtt ccatcggcat ttctaaatac aaaagccaat tgtgcaatta cttcgccttc
      781 agccaaattg taataagatg acaaatttgg aattgttaac tcccacaaat ctgtcccaat
      841 ggaaacaagt tgatatttag acgcattgtc aagccaaact ggagcttttt tccatcctgg
      901 acctgaggtt agtataaccc ctgcatgcgc ataacatgta gctgacccaa gcatggcagc
      961 attaccttga gtagcatcaa aaacaacttt tatttcgcca gcataatttt gagcaataaa
     1021 tgccggagta gtcgtaacta gttgctgagc aaaaaaagaa agactaaaaa gaaacagaaa
     1081 aaatagaaaa aaactttttt tcatacgatg atgttttcaa gtataaagac acacacctca
     1141 ctccacaaac tatgtatcat ttataaaaat gtatacaaaa acaagctgaa tgccgtctac
     1201 ttattgtgaa caaatataat taatatacaa taataattgt aaaaatcttt cgcaagatac
     1261 gaaatatttt tggataaagc aattgattag atgcatttta acaaaaaagg actcacttat
     1321 atgaatcctt ttttgttaaa atactatacc cttacttctt tttatttaac ttcttcgtct
     1381 gtatatcgta cgctaatggt acagaaacaa acaatgtaga atacgttcca atcgttattc
     1441 ctaataacaa agcaaacaca aatcccctaa ttgtatctcc accaagtaag aaaataatta
     1501 tcaaaaccaa taaggtagaa aaagtggtat taaatgttct ggataaggtt gcatttaccg
     1561 aatagttaaa catttctttt ctatcccgtt tcggatacaa cccaatcgtt tctctcaatc
     1621 ggtcgaatac tactaccgta tcatttaccg aatacccaat tacggtaagt atagcagcaa
     1681 taaatgattg tccaatttcc atagaaaaag gcatcacact atagaacaat gagtacactc
     1741 ccaatacaaa tacagaggta tgaattaaag aaaccacaat acttgctgaa taagatatat
     1801 ctttaaatcg caaaagaata tacaatccaa taccaatcaa tgaaaagaag atagccaagt
     1861 aggctgcatt tttgatgtcg tcagcaatag ctggaccaac tttttgcgaa ctttgcacgc
     1921 ctaatggacg cactgaacca gcactagaca attgggcatc gccatcttca tttataacat
     1981 accccttcac aaacatatca cgagtaactg tttgattctt gataaatggc tttaattgtt
     2041 catacaagac attttcaatt tcatcgtcca cggtatcact gttatcttga attttatagt
     2101 ttgtagatat ccgcacttga ttttcagaaa cagcaccaat agtaattaca gatacagaag
     2161 catcctcaaa accaggagcc actttacgtt gtaggtcttg tgtacttacg ctttcttcaa
     2221 attttatcac gtaattacga ccaccagtaa aatcaattcc ttgattcaaa ccatggaaag
     2281 ccaacgagaa tatagccact accataaagg taatagaaag aatatagcct actttacgta
     2341 atttcaccca atcaagattg acgttactta aaaaattggc tgaaactttg gttgtaaaag
     2401 ataatttttc tgtaaaattt ttatccaata acgtttcgta agcccaacga gtaacaaata
     2461 cggctgtaaa aaatgatgta acgataccta taatcagtgt agtagcaaat ccttttattg
     2521 gaccagtacc aaaataaaat aatattacgg cagtaataat ggttgtaata tttgaatcaa
     2581 agatagcaga taaagcattt ttgtaaccat cggcaattgc tgatttatgt gtttttccag
     2641 ctgcaagttc ttctttaata cgctcataaa tcaacacatt ggcatctacc gccataccca
     2701 acgtaagcac aatacctgct ataccgggca aggttaatac ggctttaaac gaggctaaaa
     2761 taccaacaag gaaaaataca ttaaataata aggctccgtc aactactaac ccaggcacaa
     2821 caccatacat aagtatcata taaagcatta cgccaataaa tgcaatagca aaagaaagta
     2881 agcccttttg aatggcttct tgaccaagag atggtcctac cacgtcttct tgaacaatac
     2941 gtgatggggc aggcatacga cccgacttca atacgttagc taagtctttt gcttcttcta
     3001 ccgtaaaatt accactaatt tcagagcgtc cacccgtaat ctcagtattt acattaggat
     3061 aagaatagac catatcatct aatacaatag caatacttct accaatattt tctttggtta
     3121 atcgtgccca cgtacgtgcc ccttctgggt tcattgtcat actcaccacg gaatttgacg
     3181 aaaactgtcc aaaatcacta cgagcatcac taatagcact gccttctaat ggagccttgc
     3241 catctctact tgtaacttta atagctatca actcaaagaa attccctttg gtatcgattg
     3301 attttacaga ccatttgaac tctacgttac gaggaagcac ttcttttact tgacgtaaat
     3361 ttaaataact ttttacgaga gctgtgtctt tttgatgagc gacaccgacg gcaggagttg
     3421 gcaccaactg accattttga acatttaatt gcaagacact gaataaggga ttttcttttg
     3481 agaaatcatc ttcagaaaga gtgtctttag gttgttcaag ttgagataac aaagagttta
     3541 cattatcttt ttgttcttct actttctctt ccgaagtaga atcagcaaca ctagtaggtg
     3601 tttcgacaac gggtatttta tctttttgag attcctttat tacattattg gcagttacta
     3661 aagactcgta cacctctggt aaggtatatg tttcccaaaa ttctaggtta gccgaacctt
     3721 gaagcagttt acgcacacgt tctggttctt ttacaccagg caattctatt aatatacgtc
     3781 cattggtttc aagacgttga atatttggtt gaactacccc aaaacggtca atacgagttc
     3841 tcaatacatt gaatgaatta tcaacagttg cttgtacttc ttttttgata acggcaataa
     3901 cttcggcatt agtactcttt agattaactt tatcctttag ttcataggtg ctaaatatag
     3961 ctgctaaacg ggcatcttta tctatttcct gaaaagcttc ttgaaacaaa tcaatgaaat
     4021 ctttttggct tgttgcttga cgttggtgtg ctaactgtat ggctttagta aatgtttcgt
     4081 cagtactatt acctgcaagg gtttttagaa tgtcgggcac tgacacttca atgataacat
     4141 tcataccgcc ttttaggtct aagcctaagt ttaattcttt ctctctacac tctttcaagg
     4201 tatagttaag ccacactttt tcagttgaaa gagaatctaa atagcggtac tcttccatta
     4261 catttccttg tgcaaactct tttgcttgtt tgtcgtaatg tgatgttaca acattaaacg
     4321 acaagtaaaa aaggcacact aagaccaacg caatggtaaa agatagtaca agtcctttgt
     4381 tttgcatgtt tgtattaaaa
//
LOCUS       DAOSMY010000104         4396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19942_length_4396_cov_2.919604, whole genome shotgun sequence.
ACCESSION   DAOSMY010000104 DAOSMY010000000
VERSION     DAOSMY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19942_length_4396_cov_2.919604"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>4396
                     /locus_tag="PLD76_03385"
     CDS             165..>4396
                     /locus_tag="PLD76_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOU27080.1"
                     /translation="MIHKKIYTLFVILLTLTANSYGNSDAIHKVADTFFNFTKDGKRK
                     AVAETKPIEVKYVSINNNKSNVFVVQQDGEGFAIISQHGDASSVVAYIPNGDFEKAKL
                     SPSFMSVLQIYENNNPETTPPLHTKWDVLGTTQPLLDQAGVSLNQFYHASLGNCPTGC
                     VATALAQIMAYHKFPSTGKGSHCYTHPQYGQQCADFENTHYNWTNPTYDDYELLSYHI
                     GVATEMNYCGSKYGSSPSRSNYSEVLELYYGYSMRKGTRDMKYLKTEIDEMRPVYLEL
                     GGDPGHAVVIDGYKDENYLHLNFGWGGQANGYYMMNTNSTFWVGYTFGTNLGSNIFYS
                     PTPFITNESDSLALIAIHNALDGTTGWNTSLPVSKWHGVITHHSRVVELGLGGYDYGV
                     KGNIAPEIALLNKLQHLTIIGSELNGNLTADIFQLPDLESLHISKNAGTLQCEFPSQI
                     DGCQKLTHLSMPGILTGTIPESIGNLVQLQELSIYNGTLSGSIPLTISQLTKLKTINI
                     SKNQLSGPIPDAFYNLVDVETLDLSENNLSGNLPISLEKMTQLKELRLNKNNLSGILS
                     DVFEHVKNISYLDVSENHLSGNVPESIGKLDKLQKLFLHNNQFVALPNDMSGLEALNE
                     LLVNNNLLTTLPLSINAISSLRTIDASNNQITTLHRDFGLLRNLQSLNISNNQLTEIP
                     SSIFLNAKLGTILLSNNKIKAFPSSIDFLSSSIDFAKFDDNELEGQIPLNLLSAKATH
                     VSLINNRFTYEDVPVSDELRHAVGLQKNHRLKQNKFPVMMGDTIRIDIRKISKLSHPD
                     NLYYWLEYPKYADKNGIAFLEQLQPNPILTVVINEKTAAKKFYCKVFNNNVPTWTFFF
                     NNYEYINPTMSVVNTDTIEFEILSEDEMMAYIHAESNFIQSKQILNKTISDHMVTLVP
                     PLTTYRGSLVWQASTDGIAWHSITNTMANETIKGNIVSIKKDSLVISPLADAFYRTAI
                     VESDCNPLYSDTVHIKAYGKLLYDKEINTSNDDVKIELDDIEVLVPKGIHNTDFRITI
                     VEVANPPAKPDNALKMSPVYDINVSFANEFYLPISIKFKNIDKESFDHKNIDTYVAMY
                     FDDIAQEWVPFDKANISLQDTTLHLATYHFTKLAWFEIAHGSYTHIFTSKYVNVIYKY
                     GDGYPESNFYIRYNRQTDNQGRQYWHDTNNNPDADPYGTPYLIQDVAHYMDSIIHKFK
                     NMGLETPSLRFNVYVDLIGNNGMIDAGGYLAGRGYFYIDPQYVSYFDNQEENRHEMMR
                     TLAHEYMHYTQDYYMTVLLQNYYWMEATAPIAARIVWEENSLPDTEPELLIQDALYPE
                     RDNTNLVQRILMMGRNKTIFETLSDSWLSNYNIPVATKLRSNSADANIASLFLHYMQN
                     YREGKKLNATSLIKETSYLETWIEYLTSFVNK"
BASE COUNT         1529 a          806 c          737 g         1324 t
ORIGIN      
        1 ttattgatat aaacggaaat acatgtattg agacgtcaat tacaggttct acttatttac
       61 ctgtttcttt ggcaagtggc ttacatatag tattaataca gtatggagat aatgtgtggg
      121 ttgaaaaaat tatggttgaa taatgtattg caaagaaaat gagtatgata cataaaaaaa
      181 tatatacctt attcgttata ctattgacac taacagccaa tagctatggc aatagtgatg
      241 ctattcataa ggtagctgat acgtttttca atttcactaa agatggtaaa agaaaagctg
      301 ttgcagaaac aaagccaata gaagtgaaat atgtatcgat aaataacaac aaatcgaatg
      361 tttttgttgt gcaacaagat ggagaaggat ttgccatcat tagtcaacat ggtgacgcca
      421 gtagtgtggt tgcatatatt cctaatggcg attttgaaaa agccaaatta tcgccttcgt
      481 ttatgtctgt gttgcaaatc tacgaaaaca ataatccaga aacgacacct cccttacaca
      541 caaaatggga cgttttaggc actacacaac ccttgctcga tcaagcaggt gtttctctta
      601 atcagtttta tcatgcatca ttaggaaact gtccgacagg ttgtgttgct actgcattag
      661 cacaaataat ggcatatcac aaatttccaa gcacaggtaa aggtagtcat tgctacacac
      721 acccacaata tggacaacaa tgtgctgatt ttgaaaatac tcactataat tggactaacc
      781 ctacgtatga tgattacgaa ctgttatctt atcatatcgg tgtagctacc gaaatgaatt
      841 attgtggttc gaaatatggc agttctccat cacgttccaa ctactcagaa gttcttgaat
      901 tatattacgg atattcgatg agaaaaggaa ctcgcgatat gaaatacctt aaaactgaaa
      961 tagatgaaat gcgtcctgta tacctcgaat taggtggcga tcctggacac gcagtagtta
     1021 tagatgggta caaagatgaa aattatttac atttaaattt tggttggggt ggtcaagcca
     1081 atgggtatta tatgatgaat accaatagta ctttttgggt tggttatacc tttggcacca
     1141 acttaggaag caatattttt tattccccta ctccttttat cacaaacgag tctgattctt
     1201 tagctcttat tgccatacac aacgcactcg atggtactac agggtggaat acgtctttac
     1261 ctgtatcgaa gtggcacgga gtaataacac atcattcaag ggtggtagaa ttaggattgg
     1321 gtggatacga ttatggggta aaaggaaata tcgctcctga aattgctcta ttaaacaaat
     1381 tacaacattt aacaatcatt ggtagcgaac tcaatgggaa tcttacagct gacatttttc
     1441 aactaccaga ccttgaatcc cttcatattt caaaaaatgc gggcacatta caatgtgaat
     1501 ttcctagtca aattgatggt tgtcagaaac taactcactt atcaatgcca ggtatcctca
     1561 ctggaaccat acctgaatcc atcggaaacc tagttcaatt acaagaatta tctatttaca
     1621 atggaactct ttctggaagt attcctttaa caataagtca attaaccaaa cttaaaacaa
     1681 taaacataag taaaaaccaa ttaagtggac ccattcctga tgctttttac aacttggtag
     1741 atgtagaaac tctcgattta tctgaaaaca acctttcggg gaatctgcct atttctctcg
     1801 aaaaaatgac tcaattgaaa gaattacggc taaacaaaaa caacctaagt ggtatcctat
     1861 cagatgtgtt tgaacatgta aagaatatta gttatttaga tgtatccgaa aatcatttat
     1921 ctggaaacgt gcccgagagt attggtaagc tagataaact acaaaaactg tttttacata
     1981 acaaccaatt tgtggcatta cccaacgata tgagtggatt agaagcattg aatgaattac
     2041 tcgtcaataa taatttactc acaacattac ccttgagtat caatgctatt tcaagtttac
     2101 gcacaatcga tgcctctaac aaccaaatca ctaccttgca tcgtgatttt ggcttattaa
     2161 gaaaccttca atcattgaat atatcaaaca accaacttac agaaatccca agctcaatat
     2221 ttttaaatgc taaactaggt acaatcctat tatccaataa taaaattaaa gcatttccgt
     2281 catctataga ttttttatct tcatctatcg actttgcaaa attcgacgac aatgaattag
     2341 aaggacaaat acctcttaat ctactcagtg caaaagcaac acatgtatcg ctcataaaca
     2401 atcgatttac gtatgaagat gtacctgtat cagacgagct acgtcatgca gttgggttac
     2461 aaaaaaacca tcgtttaaaa caaaacaaat tcccggtgat gatgggtgat accatccgta
     2521 tagatatcag aaaaattagc aaattatctc acccagacaa tctttattat tggttagaat
     2581 atccaaaata tgcagataaa aatggaattg cctttttaga acaattgcag ccaaatccaa
     2641 ttttaacggt ggttatcaac gaaaaaacag cagccaaaaa gttttattgc aaagtgttta
     2701 acaataacgt acccacttgg acattctttt ttaataatta cgagtacatc aacccaacaa
     2761 tgagtgttgt aaatacagat actattgaat ttgaaattct ttcggaagat gagatgatgg
     2821 catatataca tgctgaaagt aattttatac aatccaaaca gatactaaac aaaacaattt
     2881 cagatcatat ggttactttg gttcctccat taaccactta cagaggttcg ttggtgtggc
     2941 aagcttctac agacggaatc gcatggcact ctatcaccaa cacgatggca aatgaaacca
     3001 ttaaaggaaa tattgtttct attaaaaagg attctttggt tatttctcca cttgctgatg
     3061 ctttttaccg tactgccatt gttgaatccg actgtaatcc tttgtatagc gatacagtac
     3121 acataaaagc ttatggcaaa ttattgtacg ataaagagat aaacacaagc aatgatgatg
     3181 taaaaattga gttagatgac attgaagtat tagttcctaa aggcatacac aataccgatt
     3241 tccgcatcac catagtcgaa gttgctaatc ctcctgcaaa acctgataat gcactcaaaa
     3301 tgagtcccgt ttacgatatt aatgtaagtt ttgctaacga attttatcta cccatttcaa
     3361 ttaaattcaa aaatattgat aaagaatcct ttgaccacaa gaacattgat acatacgtag
     3421 caatgtattt cgatgatatt gcccaagagt gggttccttt cgacaaagcc aatataagtt
     3481 tacaagatac gaccttgcat cttgccacct accattttac taaattagct tggttcgaaa
     3541 ttgctcacgg atcctataca catattttta cttccaagta tgtaaacgtg atctataaat
     3601 acggtgatgg atatcctgaa tcaaattttt acataagata taataggcaa acagataatc
     3661 aaggtcgtca gtactggcac gatacgaaca ataaccctga tgcagaccca tatggtacgc
     3721 catacttgat acaagacgta gctcattata tggattcaat cattcataaa tttaaaaaca
     3781 tgggactcga aacacccagt ttacgtttta atgtgtatgt tgacttgatt ggtaataatg
     3841 gaatgataga tgctggaggc tatttagctg gtcgtggata tttttatatt gacccccaat
     3901 atgtttcata ctttgataac caagaagaaa atagacacga gatgatgcgt acattggctc
     3961 atgaatacat gcattataca caagattatt acatgactgt tttgttacaa aattattatt
     4021 ggatggaagc aactgctccg attgcagcac gtattgtatg ggaagaaaat tcgctacccg
     4081 acactgaacc cgaactactc attcaagatg ctctttatcc tgaacgagat aataccaatt
     4141 tggtacaacg aattttgatg atgggtcgca ataaaactat ctttgaaacc ttgagtgatt
     4201 cttggctttc aaattacaac atacctgtgg caaccaaact tcgatcaaac tcagctgatg
     4261 ccaatatagc ttccttgttt ttacactaca tgcaaaatta ccgtgaagga aaaaagctaa
     4321 acgctacatc gctcataaaa gaaacaagtt atttagaaac gtggattgaa tacttaacta
     4381 gctttgtaaa caaaca
//
LOCUS       DAOSMY010000105         4395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_19946_length_4395_cov_4.043318, whole genome shotgun sequence.
ACCESSION   DAOSMY010000105 DAOSMY010000000
VERSION     DAOSMY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19946_length_4395_cov_4.043318"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLD76_03390"
     CDS             <1..260
                     /locus_tag="PLD76_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU27081.1"
                     /translation="ELKNASYSSGEIANLVSLTHSNTSKVIATLEKKQLVTRIIGDTD
                     KRKMFFSITPQGTQKVNELLCEESEIQRIAKNIESDILQEK"
     gene            425..793
                     /locus_tag="PLD76_03395"
     CDS             425..793
                     /locus_tag="PLD76_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOU27082.1"
                     /translation="MKAIYTILLLLAANIFMTFAWYGHLKLKDFKWFSALPLIAVILF
                     SWGIALFEYFFQVPANKIGFRENGGPFTLLQLKVIQEVITLVVFVAFSSLFFKTETFR
                     WNHAIGFMFLVLAVYFIFKK"
     gene            905..1243
                     /locus_tag="PLD76_03400"
     CDS             905..1243
                     /locus_tag="PLD76_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU27083.1"
                     /translation="MNSENIKSQMRKGYLEFCILLILEKKPAYASDILNELKAAKLIV
                     VEGTLYPLLTRLKNSGYLDYNWIESLQGPPRKYYELTENGKQFLSEIEEAWQEIKNVV
                     EHIKQQPNNE"
     gene            1297..2961
                     /locus_tag="PLD76_03405"
     CDS             1297..2961
                     /locus_tag="PLD76_03405"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOU27084.1"
                     /translation="MKKTVTINLNGIVFHIDEDAYMLLQHYLEQLSKQFSAEDEKEIL
                     NDIEARIAELFTEKLQNGKNVVEKPDVERVMEVLGNPSQFADNDNDDTDNQHAENDKR
                     KRTNKRLYRDPENKILGGVAAGIAAYLEWNILLVRLLFVLFLFISVGWSFLLYLVIWI
                     IVPEATTTAQRLEMHGEDVTIENIKTQVKNAQEYVQSDEFKSNAKNIGSRIERILSPI
                     VKTVFIVVSSIVGFTLLLVSLSLLAAFVIALLHADILLAFLPFSVGSGYLAIIGIVSI
                     FLLIITPTIGLFVGAIRLLSNKTPRKGKSVFGWILFTVWVISLVALISVVVRGTLEYK
                     PSYTRSFAHYPAENRTLDGFHTILLHNGVHAEYTQDSTQQVQIMAPDYTLQNIHTEVN
                     DSILNIYFDGETNSIFTKNFAIHIQSPTIKRIEVEGGSSITTNTAIKTSNLQLKVADL
                     SQAQLLLDVEKNVNVISTGLSRVQLSGNTHHLDLNVSNASMANTIDLVSKTGTVNVQN
                     AAKADIQVRDSVWIQANDAARVCYRGNPIIKQQAYNAAVVVRYNEQ"
     gene            3241..>4395
                     /gene="htpG"
                     /locus_tag="PLD76_03410"
     CDS             3241..>4395
                     /gene="htpG"
                     /locus_tag="PLD76_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOU27085.1"
                     /translation="MKKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLK
                     TLASVGEYKGELGDITVRVTRDEANKTITISDKGIGMTAEEIDKYINQIAFSGAEEFL
                     DKYKNDAAAIIGHFGLGFYSSFMVAKKVEIITKSYKENSQAVKWSCEGNPDYTLENCD
                     KAERGTDIVLHIDDENQDFLQQSKINELLTKYCKFLPIPIAFGKKTDWKDGKQVDTDE
                     DNIINDTQPAWVRKPADLKDEDYNKFYRDLYPMSEDPLFHIHLNVDYPFNLTGILYFP
                     KVKNTIDLQKNKIQLYCNQVFVTDSVEGIVPDFLTLLHGVIDSPDIPLNVSRSYLQSD
                     GNVKKISSHITKKVADRLQDLFKTDRALFEEKWESLKIFINYGMLTDDKFY"
BASE COUNT         1537 a          813 c          762 g         1283 t
ORIGIN      
        1 gcgaattgaa aaatgccagc tactcttctg gagaaatagc caatctagta tcattaacac
       61 attctaatac atccaaggta atcgcaacac ttgaaaaaaa acaactagta acacgcataa
      121 taggtgatac tgacaaaaga aaaatgtttt tttcaatcac cccacaaggg acacaaaaag
      181 taaacgaact tctttgtgag gaaagtgaaa ttcaacgtat tgcgaaaaat attgaatcag
      241 atatcttaca agaaaaataa gaagaaattt gtttcacact ctatcctttt ttacaggcat
      301 attcgtgtca tgtaaaaaag gattttttgt atcataaaat gatggttgat tatacaaaaa
      361 atacaaaaac tttgtactac atttgcacaa tttttagtgc atcttctttt aataaaagaa
      421 ctatatgaaa gccatttata ccattctttt gttgcttgct gccaatattt ttatgacttt
      481 tgcttggtat ggtcatttaa agttgaaaga ttttaaatgg ttttcggcat tgcctcttat
      541 tgctgttatc ttatttagtt ggggaattgc tcttttcgag tatttttttc aagttcctgc
      601 caataaaatt ggctttcgcg aaaatggagg accctttacc ctgctccaac tcaaagtgat
      661 tcaagaagta attaccttag ttgtttttgt tgctttctct agcttattct tcaaaaccga
      721 aacctttcgc tggaaccacg ccataggctt tatgttcttg gtattggctg tgtattttat
      781 tttcaaaaaa taaattgcct tctatttttt tgttaatatt tcttaattta tagtagtatg
      841 tattacatag tactataaac acacttatct ttgcactatc aacttacata caaacgctat
      901 gtcaatgaat tcggaaaata ttaaatcgca aatgcgaaaa ggatacctag aattttgcat
      961 tctacttatc ctcgaaaaaa aacctgccta cgcatcggac atactcaacg aacttaaagc
     1021 cgctaaacta atagtagtag agggcacatt gtatcctttg ctaactcgac tcaaaaacag
     1081 tgggtatctt gactataact ggatagaatc gctacaaggt ccaccccgta aatactacga
     1141 actgacggaa aacggcaaac aatttttgtc cgaaatagaa gaagcttggc aagaaataaa
     1201 aaatgtagtt gaacacatca aacaacaacc taacaacgaa tagacagaac acttcatcat
     1261 caataacaat ccctcaaact acaaacatat actcccatga aaaaaacagt aacaatcaac
     1321 ctcaacggca tcgtttttca tatagacgaa gatgcgtaca tgttgcttca acattactta
     1381 gagcaattaa gtaaacaatt tagtgccgaa gatgaaaaag aaattctcaa cgatattgag
     1441 gctcgtattg cagaattatt taccgaaaaa ttacaaaacg gtaaaaatgt agtcgaaaaa
     1501 cctgatgtag aaagggtaat ggaagtgctt ggcaatccta gccaatttgc tgacaacgac
     1561 aatgatgata ctgacaatca acacgctgaa aacgataaaa gaaaaagaac taacaaacgt
     1621 ttataccgtg atccagaaaa caaaatacta ggtggtgtag cagcaggtat agctgcctat
     1681 ttggaatgga acatattgct tgtacgtttg ctatttgtgc tatttctatt tatcagtgtt
     1741 ggttggtcat ttttactata cctcgttatc tggattatcg tgccagaagc aactactact
     1801 gcccaacgac tcgaaatgca tggcgaagat gtaacgattg aaaacattaa aacgcaagtt
     1861 aaaaatgccc aagagtatgt tcaatcagac gaatttaaaa gcaatgccaa aaacattggc
     1921 tctcgcattg aaagaatact ctctcctatt gttaaaacgg tattcatagt cgttagttct
     1981 attgttggtt ttaccttgct acttgtatct ttatcgttgc tagcagcatt cgttatagca
     2041 ttgctacatg cagatatatt acttgcattt ttaccattta gtgttgggtc ggggtattta
     2101 gcaattattg gcatagtgag tattttctta cttattatta cgccaactat cggactattt
     2161 gttggagcca ttcgcttatt atcaaacaaa acacctcgta aaggtaaaag tgtattcggt
     2221 tggattttgt ttacagtatg ggtcatcagt ttagtagcac tcatctctgt agtagtaaga
     2281 ggcacactgg agtataaacc ctcctataca aggtcttttg ctcattaccc tgccgaaaat
     2341 cgtaccctag atggctttca taccatctta ttgcacaatg gcgtacatgc tgagtacaca
     2401 caagactcta cacaacaagt acaaattatg gctcccgatt acaccttaca aaacatccat
     2461 acagaagtaa atgatagcat attgaacatc tattttgatg gagaaacaaa ttcaatcttt
     2521 actaaaaatt ttgccataca catacaatcg ccaaccataa aacgcattga ggtagaagga
     2581 ggctcaagca ttacgacaaa cacagccatt aaaacaagta atttgcaact gaaagtagct
     2641 gatctaagtc aggctcaact actgctagat gtagaaaaga atgtaaacgt gattagtact
     2701 ggcttatcgc gggtacagct atcgggcaat acgcatcacc ttgacttaaa cgtaagcaat
     2761 gccagcatgg caaacacaat tgatttagtg agcaaaacgg gcactgtcaa tgtacaaaat
     2821 gcagccaaag cagatataca agtacgcgac agtgtgtgga tacaagccaa cgatgctgca
     2881 cgtgtatgct atcgtggcaa cccaatcatt aaacaacagg catacaatgc tgcggttgta
     2941 gtacgctata acgaacaatg acatgtattt gaacacaaaa gagcggtcaa aatttgaccg
     3001 ctctttgtga ttttcatagg tactcgacac aaaatcctag gctacaagta tttcgaatgt
     3061 cagacactac aaagatgata cttttttttg aattctgaaa tagcgtagta tatgttatac
     3121 aatatgtttt ttagttaaaa ttacacatcc actatgcctt tttgacagac agatatactt
     3181 ggcactactt ttgcatagct aaagtgaaac aaatgtataa cttaaaataa atagttgagt
     3241 atgaaaaaag gaaatattgg tgtaactacg gaaaacattt tccctatcat taaaaaattt
     3301 ctttacagcg accacgaaat ctttttacgc gaactagttt ctaatgctgt tgatgctaca
     3361 caaaagctaa aaacattggc atcagttggc gagtacaaag gggaattggg cgacattact
     3421 gtgcgtgtta ccagagacga agccaacaaa accattacca tttctgacaa aggtattggt
     3481 atgactgccg aagaaattga taaatatatc aaccaaattg ctttttcggg agcagaagaa
     3541 tttttagata aatacaaaaa cgatgccgcc gctatcattg ggcattttgg tttgggtttt
     3601 tactcttctt ttatggttgc caaaaaggtg gaaatcatca ccaaatcgta caaagaaaat
     3661 agccaagcag taaaatggag ttgcgaaggt aaccccgact acacattaga aaattgcgat
     3721 aaagcagaaa gaggtacaga tattgtattg cacattgatg acgaaaacca agacttcttg
     3781 caacaaagca aaataaacga attgctaact aaatactgca aatttttacc tatccccatt
     3841 gcctttggca aaaaaacgga ttggaaagat ggcaaacaag tagatacgga cgaagataac
     3901 atcataaacg acacacaacc agcttgggta cgcaaaccag ccgatttgaa agacgaagat
     3961 tataacaaat tttaccgcga tttgtatcct atgagcgaag atccgctctt tcatatacac
     4021 ctaaatgtgg attatccgtt taacctaacc ggtatattat actttccaaa agtaaaaaat
     4081 accattgatt tgcaaaaaaa caaaattcaa ttgtattgca accaagtgtt tgtaaccgac
     4141 tcagtagaag gtattgtgcc cgatttctta accctattgc acggagttat cgactctcca
     4201 gatattccgc tgaatgtaag tcgctcgtac ttgcaaagcg atggtaatgt gaagaaaatt
     4261 tcgagtcata ttaccaaaaa agtagctgac aggttgcaag atttgtttaa aactgaccgt
     4321 gctctatttg aagaaaaatg ggaaagccta aaaatattta tcaattacgg tatgcttact
     4381 gatgataaat tttat
//
LOCUS       DAOSMY010000106         4384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20022_length_4384_cov_3.298452, whole genome shotgun sequence.
ACCESSION   DAOSMY010000106 DAOSMY010000000
VERSION     DAOSMY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20022_length_4384_cov_3.298452"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /gene="mutS"
                     /locus_tag="PLD76_03415"
     CDS             complement(<1..2352)
                     /gene="mutS"
                     /locus_tag="PLD76_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU27086.1"
                     /translation="MKQYNQVKAKHPDAVLLFRVGDFYETFSDDAIRVSEILGITLTR
                     RANGAASYVELAGFPHHALDTYLPKLVRAGLRVAICEQLEDPKTAKGIVKRGITELVT
                     PGVSYNETTLNFKENTFVAAVHFEKSRVGVAFLDISTGEFLTAEGSSEYVDKLLTSFS
                     PKEVLYERGKTSLFEQSFGTHFLSFTLEDWVFVSDTANERLQKQFQTKNLKGFGVVDL
                     PLGIIASGAILHYLEFTNHLQTAHITHLRRIEEERFVWLDRFTIRNLELYQAMHDGGK
                     SLLDVLDKTLTPMGARMLKRWLAFPLKNKAAIEHRLSIVSYFFKDKDIQQVLYQQLSL
                     IGDLERLVSKIAVGRINPRELVQLKIALTAVETIKNSSTLVDNEALVSIAERLQSCTR
                     MRDKIEQTIASDAPMLLHKGGVIKQGIHPELDEYRRLAYSGKDYLLQIQQRESEATNI
                     PSLKISYNNVFGYYIEVRNTHKDKVPSNWIRKQTLVSAERYITEELKEYEEKIVGAEE
                     KIAQIEGQLFNELVVSLCDYISALQLNASLIAELDCLLSFANVSANNSYIPPVITETD
                     VLSIKQGRHPVIEKQLPMGESYIANDVYLDTNTQQIMMITGPNMAGKSALLRQTALIV
                     LMAQIGCFIPAESAEIGIVDKIFTRVGASDNISMGESTFMVEMSEASSILNNVSPKSL
                     ILFDELGRGTSTYDGISIAWAIVEYLHEYPQAKAKTLFATHYHELNEMEKTFSRIKNF
                     NVAVKEMDNKIIFMRKLVPGGTEHSFGIHVAKMAGMPKSVVKRS"
     gene            complement(2627..2896)
                     /gene="rpsO"
                     /locus_tag="PLD76_03420"
     CDS             complement(2627..2896)
                     /gene="rpsO"
                     /locus_tag="PLD76_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU27087.1"
                     /translation="MQLTKEQKQEIFEKYGKSNTDTGSSEGQIALFSARIAHLTEHMK
                     SNHKDYSTERALVMLVGKRRRLLDYLKAKDIERYRAIIKELGIRK"
     gene            3074..>4384
                     /locus_tag="PLD76_03425"
     CDS             3074..>4384
                     /locus_tag="PLD76_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU27088.1"
                     /translation="MKSFKTMNTLFGWISFLIAWVVYALTIEPTASFWDCGEFITSAF
                     KLQVGHPPGAPFFNLTGNFFTLFAKDPSQVAIMVNLMSATLSAFTILFLFWTITHIAK
                     KIIVQNGSEITLSQSITIIGSGLIGSLVYAFSDTFWFSATEAEVYAYSSFFTAIVFWL
                     ILKWEDQHDKAGSDKYLILIAYLMGLSIGVHLLNLLTIPALVLVYYFRKTEKVSLKGG
                     TWALLISFAILGFIMYGFIPQFIANAGIAELFFVNTLGFSFNTGAFFYYFTTIAVLVW
                     AIIELYSKKPSATRIKVSFIGSLILLGIPFLLGSLLLGLVLLSILIFYFIKLKEKISI
                     RLLSTIIISLTMMLIGYSSYALIVIRSAANPPMDQNSPDNIFALQSYLNREQYGDRPL
                     FYGAYYSAEPKLIEEDGYCKPSVKEGAKTWGQAEKTSPNEKDRYI"
BASE COUNT         1373 a          852 c          695 g         1464 t
ORIGIN      
        1 agagcgtttt accacactct ttggcatacc agccattttt gctacatgaa taccaaaact
       61 atgttctgta cctcctggca ctaatttacg cataaaaata atcttgttat ccatttcttt
      121 tacagcaacg ttgaagtttt ttattcgcga aaaagtcttt tccatctcat ttaattcgtg
      181 ataatgggta gcaaataatg ttttggcttt tgcttgagga tactcgtgca agtattccac
      241 tatagcccaa gcaatagaaa tcccatcgta ggtactggtt ccccttccca gctcatcgaa
      301 taaaattaaa cttttgggcg atacgttatt tagaatactg gatgcttcgc tcatttcgac
      361 cataaacgta gattcgccca ttgagatatt atcgctagca ccaactctag taaaaatttt
      421 atctacaata ccaatttcgg cactctcggc agggataaaa caacctattt gtgccattaa
      481 tacaataaga gctgtttgtc ttaataaagc tgatttacca gccatattgg gtccagtaat
      541 catcattatc tgttgggtat tggtatctaa atacacatca ttggcaatat aggactcccc
      601 cataggaagt tgtttttcga taacgggatg acgcccctgt ttgattgaca acacgtccgt
      661 ttcagtaata actggaggaa tataggagtt attagctgac acattcgcaa aagacaacaa
      721 gcaatctaat tctgcaatta aggaagcatt cagttgcaag gcactaatat aatcgcacaa
      781 cgatacaact aactcattga aaagttgtcc ttctatctgg gcaatttttt cttctgcccc
      841 cacaatcttt tcttcgtatt ctttcaactc ttcagttata tagcgttctg cactgactaa
      901 ggtttgctta cgaatccagt ttgagggaac tttatcttta tgcgtattac gtacttcaat
      961 atagtagcca aatacattgt tataacttat ttttaggcta ggtatattgg ttgcttcact
     1021 ttctctctgt tgaatttgta ataaataatc tttacccgaa taagctaaac gtctatattc
     1081 atccaattca gggtgaattc cttgtttaat tacaccccct ttatgtagta acataggagc
     1141 atccgaagca attgtttgtt caattttatc tcgcattcgt gtacacgact gcaaacgttc
     1201 ggctatagat actaatgcct cattatcaac aagtgtactt gaatttttaa ttgtttcaac
     1261 agctgttaac gcaattttaa gctgcaccaa ttcacgagga ttgatgcgtc caacggctat
     1321 tttagacacc aaacgttcta aatctccaat taacgaaagt tgttgataca atacctgttg
     1381 aatatctttg tctttaaaaa aataactgac aatactcaat cgatgctcta tggctgcttt
     1441 attttttagc ggaaaagcta accatctttt caacatacga gcacccatag gagttaaggt
     1501 tttatctagc acatccaata atgattttcc accatcgtgc atggcttgat acaattcgag
     1561 gttacgaatt gtaaatctat ctaaccaaac aaaacgttct tcctctattc tacgcaaatg
     1621 agtaatgtga gccgtttgta aatgattggt aaactccaaa tagtgtaaaa tagctcccga
     1681 agcaataatc cctaaaggta aatcaactac cccaaaacct tttagatttt tcgtttgaaa
     1741 ttgtttttgt aatcgttcgt ttgctgtgtc tgacacaaat acccagtctt ctaatgtaaa
     1801 tgaaagaaaa tgggtaccaa aagattgttc aaaaagactt gtttttccac gttcgtacaa
     1861 aacttctttt ggagaaaaac tcgttagaag cttatctaca tattccgagg atccttctgc
     1921 cgttaaaaac tctcccgtag agatatctaa aaaggcaacg ccaacccgtg atttctcaaa
     1981 atgaacagca gctacaaatg tattttcttt aaaattgagc gtcgtctcgt tatacgaaac
     2041 tcctggcgtt accaattcag taatgcctct tttgacaatc cctttggcag tttttggatc
     2101 ttctaactgc tcacaaatag caacgcgcaa accagctcgt actaattttg gtaaatacgt
     2161 atctaaggca tggtgcggaa accctgctaa ttctacataa ctagcagctc cgttagcacg
     2221 acgtgtaagc gtaataccta aaatttcaga aacacgtata gcgtcatcag aaaatgtttc
     2281 gtaaaaatcg ccaacacgaa acaacaacac agcatcagga tgtttcgctt tcacctgatt
     2341 atattgtttc attaaagggg tttctacaac ttttgccaca aaatcttaca tttgaaatac
     2401 aaaacacaaa gataaaaaat atcaaggaag aaatgttatt ttagtaaaac aaatttaatt
     2461 aagaacgtat aatcatactg ataaatccat tacacttagt ccaatcacca aatattaacg
     2521 gtattattat cattttcagt caagttgaat tcctcaaaaa acagagaaca acgcataaaa
     2581 aaagtccgaa tgaagattcg gacttttttt gttaaaagga catcaattac tttctgatac
     2641 ctaattcttt gataatggca cgataacgtt caatatcttt tgcttttaaa tagtcaagca
     2701 aacgacgacg ttttcctacc aacattacta aagcacgttc tgtgctataa tctttgtgat
     2761 ttgacttcat gtgttcagtc aaatgagcta tacgggctga aaataatgct atctggcctt
     2821 ctgatgaacc agtatcagta ttcgactttc cgtatttctc aaagatttct tgtttttgtt
     2881 cttttgttaa ttgcatacaa tttattctaa gagattatta aaaaatgtcc gcaaagatac
     2941 ataatagttt tttagttttc aacaatattt cttaaaaact ttcagaatca ttcatttcct
     3001 acttgtattt gggtaaaatt tagtactttt gtgaggcttt ttttaaggga aatcagaact
     3061 aatcaggaaa aaaatgaaat catttaaaac catgaacacc ctatttgggt ggataagttt
     3121 cttaattgca tgggtggtgt atgcccttac tattgaacct acagctagct tttgggactg
     3181 tggtgaattt ataacctccg cctttaaact gcaagtggga cacccacctg gggctccttt
     3241 ctttaattta acaggtaatt ttttcacgct atttgcaaaa gatccttctc aagtagccat
     3301 catggtaaac ctaatgtcag caacattaag tgcgtttact attttatttt tgttttggac
     3361 aattacgcat attgcaaaaa aaatcattgt tcaaaatggg tcagaaataa cactaagtca
     3421 atctattaca ataatcggaa gcggattaat cggatctcta gtctatgctt tctcggacac
     3481 attttggttc tcagccactg aagccgaagt atatgcttat tcttcctttt ttacagctat
     3541 tgttttttgg cttatattaa aatgggaaga ccaacacgat aaagcaggtt cagataaata
     3601 cctaattctt atcgcttact taatgggctt atctattggt gttcacttac ttaacttatt
     3661 aactatacca gctttagttt tggtatatta tttcagaaaa acagaaaaag tatcattgaa
     3721 gggtggaaca tgggcactac ttatttcatt tgctatactt gggttcatca tgtatggttt
     3781 tatccctcaa tttatagcaa acgcaggcat tgccgaatta ttctttgtca ataccttagg
     3841 ttttagcttt aatacaggag cttttttcta ttattttact acaattgccg ttttagtgtg
     3901 ggctatcatt gaattatata gcaaaaaacc atctgccact cgcattaaag tttcattcat
     3961 cggatctctc attttattag gtatcccttt tctattaggc agtttgttac tagggcttgt
     4021 attactcagt atactcattt tttattttat aaaattgaaa gaaaaaatta gtattcgcct
     4081 attgagcacc atcattattt cgttaacaat gatgctaatt ggctattcaa gttatgcact
     4141 aattgttatt cgttcagccg ccaatcctcc catggaccaa aactcacccg acaatatttt
     4201 tgcgttacaa agctatctaa atcgcgaaca atatggtgat cgaccattat tttacggagc
     4261 atattactct gccgaaccta agttaatcga agaagatggc tactgtaaac catctgtaaa
     4321 agaaggtgca aaaacatggg gtcaagcaga aaaaacatcc cctaatgaaa aggacaggta
     4381 tatc
//
LOCUS       DAOSMY010000107         4384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20023_length_4384_cov_3.041580, whole genome shotgun sequence.
ACCESSION   DAOSMY010000107 DAOSMY010000000
VERSION     DAOSMY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20023_length_4384_cov_3.041580"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLD76_03430"
     CDS             <1..559
                     /locus_tag="PLD76_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU27089.1"
                     /translation="AGVLDCDSSDKAARFIRFCDSFNIPLITLEDMPGYLPGVDQEHA
                     GVIRHGAKVLYAYSEATVPKVTVILRKAYGGGYIAMNSRHLGADFVFALPSAEIAVMG
                     PEGAANIIFAKEIKEAADPDAMRAAKVQEYIDKFANPFVAAAKGYIDAVIEPNETRNL
                     LIHTLSVSKDKDDYRPEKKHGIPPF"
     gene            623..949
                     /locus_tag="PLD76_03435"
     CDS             623..949
                     /locus_tag="PLD76_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU27090.1"
                     /translation="MTEATKDSEFVDFAVTARKYKTLLTKKFINRKEWETPNPNNIKS
                     YIPGTIIELNVKPGQKVKQGELLLRLEAMKMQNRIDMPFDGIIKSVNVNVGERIPKNF
                     LMIEIE"
     gene            1079..2023
                     /locus_tag="PLD76_03440"
     CDS             1079..2023
                     /locus_tag="PLD76_03440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27091.1"
                     /translation="MLSVLIPIYNQNCSKLIYALQQQIELLHVPVEIVAIDDASTVYK
                     EENKNALNHTRHRYIELQNNIGRAAIRNTLASMAKYDYLLFMDCDTYPASDYYLHNYI
                     QCLQSNSYQNGVICGGYKYSLQPPVDTQQLFRWKYGRNREQNLAVERNKFPYKSFSSF
                     NFCIPSKVFASIQFDETIKGYGHEDTWFGIALKENKVPVIHIDNELFQVSIDKTDIYL
                     EKSRNAVKNLYTLYTTSDKMSMFEQIKLIRAFEKIKKMRLCAMGAFLYDVSKENLEKN
                     FHSHHPLLVLFDFYRLGLLCKESLLARNNSLLPNKDEV"
     gene            2013..3242
                     /locus_tag="PLD76_03445"
     CDS             2013..3242
                     /locus_tag="PLD76_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU27092.1"
                     /translation="MKYKICILFFYVSFTLFSQVVKTGIDVLQQRNFDVLSGKRVGLI
                     TNPTGVNKNLKSTVDILHEATNVKLIALYAPEHGVRGDIPAGEKVSNLIDTKTGVKVY
                     SLYGNTRKPTQEMLQGIDALVYDIQDIGCRSYTFISTLGLAMQAAAEKKIPVYVLDRP
                     NPLGGLKIEGNTTEDSFISFVSQFKIPYIYGLTVGELAYMLNEENMLGAKCELHIVPM
                     EGWKRSMRFNETELPWVLTSPHIPYMQSAVYYPASGILGELGIMSIGVGYTVPFQTFA
                     HEIFDAEKLSSSLNALRLHGVVFRPIHYKPYYGYGKDKNLKGVQVFFTNYTTARLSEV
                     QFYVLQVVKQLYPEFEVFTNENSSRWNMFDKVCGSDFIRIELQKNNYSFDKIKHYWRK
                     DEEDFKKKSTKYWIYKE"
     gene            3246..3548
                     /locus_tag="PLD76_03450"
     CDS             3246..3548
                     /locus_tag="PLD76_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HOU27093.1"
                     /translation="MDFLMHNNLIGVLIGISTFCIIGLFHPIVIKCEYYFGTRCWWLF
                     LLIGVVGICMSIIVKHILFSSLLAVFAFSSFWSILEIFEQKKRVDKGWFPKNPKKK"
     gene            complement(3581..3994)
                     /locus_tag="PLD76_03455"
     CDS             complement(3581..3994)
                     /locus_tag="PLD76_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU27094.1"
                     /translation="MAKQEEINQGISHNTIAAGTVIKGEIRSEGDVRIDGSVEGTISC
                     KGKIIIGTKGEFKGTLTAVNIDVMGLIQGEVISENLLSIKSTGKIEGEVTTKTLIIEQ
                     EAILHGNCTMISNNENKPKLDVTISPIINKESKTN"
     gene            complement(4054..4127)
                     /locus_tag="PLD76_03460"
     tRNA            complement(4054..4127)
                     /locus_tag="PLD76_03460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4091..4093),aa:Thr,seq:tgt)
BASE COUNT         1491 a          625 c          804 g         1464 t
ORIGIN      
        1 tgcaggagta ctagactgtg atagttcgga taaagcggct cgttttattc gtttttgcga
       61 ttcgtttaat ataccattga ttacattaga agatatgcca ggatatttac ctggagtaga
      121 tcaagagcat gctggtgtta ttcgtcatgg tgcaaaggtt ttatatgctt attcagaagc
      181 tactgtgcca aaagttacag taattttacg taaagcttat ggtggtgggt atattgctat
      241 gaattctcgt cacttaggcg ctgactttgt ttttgcattg ccatcagcag aaattgctgt
      301 aatgggtcca gaaggtgctg cgaatattat ttttgcaaaa gaaattaaag aagctgctga
      361 tccagatgca atgcgtgctg cgaaagtgca agaatatatt gataaatttg caaatccatt
      421 cgttgcagct gctaaaggat atatagatgc tgtaattgag cctaatgaaa cacgtaactt
      481 attaatacat acactttcgg tatccaaaga taaagatgat tatcgtcctg aaaagaaaca
      541 tggaatacct ccattttaat gtgtactcat attatatttc atagcttaag tagtattatt
      601 tagtaacaaa aaatagttag ttatgacaga agcaactaag gacagtgaat ttgtagattt
      661 tgcggtaact gcacgtaaat ataaaacttt gctgacaaaa aaatttataa acagaaaaga
      721 atgggaaact cctaatccca ataatataaa atcatatatt cctggaacaa ttattgagtt
      781 gaatgtaaaa cctggtcaaa aggtaaaaca aggcgaatta ttgttacgtt tggaggcaat
      841 gaaaatgcaa aacagaatag acatgccatt tgatggtatc ataaaatctg taaacgtaaa
      901 tgtaggagag aggataccaa aaaacttttt aatgattgaa atagaataga aaaagttgag
      961 tcagttaaaa aagtgaagtc tccaaataaa ggtagcttca cttttttttt attcatgtat
     1021 acaaatagta attttcttct tatctttatg aaccattttt agtaatactc acttttgtat
     1081 gctttctgta ttaataccaa tttataacca aaattgtagc aaattaattt acgctttgca
     1141 acaacaaata gaactgctac atgttcctgt tgagatagtt gcaattgatg atgcatcaac
     1201 agtgtataaa gaagaaaata aaaatgctct aaaccataca aggcacaggt atattgaatt
     1261 acaaaataat ataggtcgtg cagctattcg caatacatta gctagtatgg caaagtatga
     1321 ctatcttcta tttatggact gcgatacata tccagcaagc gattattatt tacataatta
     1381 tattcaatgt cttcaatcaa attcctatca aaacggcgta atttgtggcg gatataaata
     1441 ttcgcttcaa ccgccagtgg atacacagca gttatttcgt tggaaatatg gaagaaatag
     1501 agaacaaaat ctggctgttg aaagaaataa gtttccttat aaatcgttta gttcgtttaa
     1561 tttctgtata cctagcaagg tatttgcgag tatacaattc gatgaaacaa ttaaaggata
     1621 tggtcatgaa gatacatggt ttggtattgc actaaaagag aataaggtac ctgttataca
     1681 tattgataac gaattatttc aagtatctat tgataagact gatatctatc tagaaaaatc
     1741 tagaaatgct gttaaaaatc tatatacatt atacactaca tcagataaaa tgagtatgtt
     1801 tgaacaaatt aaattgataa gagcatttga aaagataaaa aagatgcgat tgtgtgctat
     1861 gggagctttt ttatatgatg tgagtaaaga aaatcttgaa aaaaattttc attctcatca
     1921 tccattatta gttttatttg atttttatcg tttaggatta ttgtgtaaag agagtttatt
     1981 agcaagaaac aacagtttat tacccaataa agatgaagta taaaatctgt atattgtttt
     2041 tttatgtatc gtttacacta ttttcgcaag tagtaaaaac aggtattgat gtattgcagc
     2101 agagaaactt tgatgtttta agtggtaaaa gagttgggtt gataacgaat cccactggag
     2161 taaataaaaa tcttaaatcc actgtggata tattacatga ggcaactaat gttaagctaa
     2221 ttgcattgta tgccccagaa catggcgtaa ggggagatat tcctgcaggc gaaaaggtaa
     2281 gtaatttgat agatacaaaa acgggtgtaa aagtatattc tttgtatggc aatacacgca
     2341 aaccaacgca agaaatgcta cagggaatag atgcattagt atatgatatt caggatattg
     2401 gatgccgttc atatactttt attagcacac tagggcttgc tatgcaagct gcagctgaaa
     2461 agaagatacc tgtatatgtg ttagaccgac ctaatccgtt aggaggatta aaaattgaag
     2521 gaaatactac ggaagactcg tttatttctt ttgtgagtca atttaaaatt ccatacatct
     2581 atggattaac agtaggagag ttggcgtata tgttgaatga agaaaatatg ttaggggcta
     2641 aatgtgaatt acatattgta ccaatggagg gctggaaacg atctatgcgt tttaatgaaa
     2701 cagaattacc ttgggtattg acttctccgc atattccgta catgcagtct gctgtatatt
     2761 atcctgcttc aggcatttta ggcgagttag gaataatgtc tattggagta ggatatactg
     2821 tgccatttca gacctttgct catgaaatat ttgatgcaga aaaactatca tcaagtctaa
     2881 atgctcttcg tttacatgga gttgtatttc gcccaattca ttataagccg tattatggat
     2941 atggtaaaga taaaaatcta aaaggagtac aagtgttttt tacaaattac acaacggcac
     3001 gtctgtcaga ggttcagttt tatgtattgc aggttgtaaa acagttatat ccagaatttg
     3061 aggtttttac gaatgaaaat agcagtagat ggaatatgtt tgataaggta tgtggtagtg
     3121 attttattcg tatcgaatta caaaaaaata actattcttt tgataaaatc aaacactatt
     3181 ggagaaaaga tgaggaggat tttaagaaga aatcaaccaa atattggatt tataaagagt
     3241 aaattatgga ctttttaatg cacaataatt taattggtgt actgataggt ataagtacat
     3301 tttgtattat tggattattt catcccatcg taattaaatg tgagtattat tttggaacac
     3361 gttgttggtg gttatttttg ttaataggag ttgtaggtat ttgtatgtct attatagtaa
     3421 aacatatttt attttcctcc ttattagctg tttttgcttt ttcttctttt tggagtatat
     3481 tggaaatttt cgaacaaaaa aaacgcgtgg acaaagggtg gtttccaaag aatcctaaaa
     3541 aaaaatagca ttgttaaaaa taaaataaag aaagcaaaac ttagttagtt ttgctttctt
     3601 tgtttataat aggggaaatg gtaacatcca attttggttt gttctcatta ttacttatca
     3661 ttgtgcagtt tccatgtaag atagcttctt gctcaataat aagagttttg gtggtaactt
     3721 ctccttctat ttttccagta gatttaatcg ataataaatt ttctgatatt acttctcctt
     3781 gaattaggcc catcacatca atatttactg cggtaagtgt tcctttaaac tccccttttg
     3841 tgccaataat aattttccct ttgcaactaa tagttccttc aacagaacca tcaattctta
     3901 catcgccttc agaacggatt tctcctttaa ttacggtacc tgcagcaatg gtattgtgcg
     3961 aaataccttg gttgatttct tcttgttttg ccatgatttt aggttttaaa atgcattaaa
     4021 tttaaaaaaa aatcccacct aaatgggatt ttgagccggc agagggattc gaacccacga
     4081 ccccgagatt acaaatcacg tgctctggcc aactgagcta tgctggcaag tgggtaagct
     4141 atctacatcg catcgctcaa ctaactaccc ttgctgcggt caaaccctgg gggattcgaa
     4201 aggagcagat cgtataggac ttacccaccg caaaagtaat gctttttgct taattgacca
     4261 agaaagtgct ttaaaaaatg acacaaaaaa ccattttttt gtaaatcaat atcgttattc
     4321 atttttttta gtattacaac ttataataat atgctaaata gttgtacctt tgtggatatt
     4381 tatt
//
LOCUS       DAOSMY010000108         4383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20031_length_4383_cov_3.037431, whole genome shotgun sequence.
ACCESSION   DAOSMY010000108 DAOSMY010000000
VERSION     DAOSMY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20031_length_4383_cov_3.037431"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1622)
                     /locus_tag="PLD76_03465"
     CDS             complement(9..1622)
                     /locus_tag="PLD76_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU27095.1"
                     /translation="MEKHKLLYPIIIGISIAVGILLANLYYQQAEQTTGFAIPKKVQG
                     NKFDEIMYLLDNNYVDVIDKNALTEEFIPQILKFLDPHSAYLTPEEVAEATELQGSFS
                     GIGVQFNIQNDTVMIVSVIPGGPSEKLGILPGDRIIAVNDSSFVGADVTNATVLKTLR
                     GEKGSIVKVRIKRNGSPLPLDFDIVRGDVPVKSIDVVYVIADSTGYVKISNFGANTYR
                     EFLDALVILREQECNNIIVDLRNNSGGYLSAVIQMTNEFLKRGQLIVYTEGVNQARED
                     AIADGRGNFRKENICVLINEWSASASEIFAGAIQDHDRGWVIGRRSFGKGLVQQQIPL
                     SDGSELRLTVARYHTPSGRSIQKPYKSGESDENYDVEIEKRVLHGEMFQKDSISFADS
                     LKYYTAKGRTVYANGGIMPDIFVPHDTTYYTPYYTNVLNNGIVYDFSFAFVDKHRAKL
                     STYDTWEKLVEYLVKKTTYFSEFIRYAEQKGVSRNSQEIAKSKALLANLLQAYIARNL
                     LGDEAFYPILNLQDNTVKKAIEMVGKPIQ"
     gene            complement(1626..2051)
                     /locus_tag="PLD76_03470"
     CDS             complement(1626..2051)
                     /locus_tag="PLD76_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOU27096.1"
                     /translation="MNTKQLVLDKRYMQMALIWAENSYCKRRQVGAILVKDKMIISDG
                     YNGTPCGFENICEDESFKTKPYVLHAEANAITKIARSHNSSEGATLYVTTSPCIECAK
                     LIIQSGIVRVVYANEYHVTDGVDLLVRAGVEVLKIDLDN"
     gene            complement(2090..3232)
                     /locus_tag="PLD76_03475"
     CDS             complement(2090..3232)
                     /locus_tag="PLD76_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU27097.1"
                     /translation="MKQSILFLFVVFVCLLSSCSQTEEYTIQGTVSGVETDTLFLEKV
                     ELGKIEIVAYQVLKKNGKFSFSFPRAVEPHYVRLRLQQGVLYLVADSTETISVYSNKQ
                     TFGQADSIVGSVASKRMQSIVHYVAQTQRKMTASQDKYQRGKIVAQDFTEETTQLVRQ
                     LKDFLRKEVYQDTRSSVAYFALYQQINGYYVFNPYDKEDVKVYAAVATPLQVHYPQTA
                     RTKNIEDVVLKGMQRRKNAEDKLVREIVGKGFIDISLSTVQGVERSLSASLGRATIVD
                     FSLFQSENSYAHTVLLQELYRVYASKGLRIYQVSFDDDAVFSVVAKQLPYTVVHDKKG
                     LVSPLLQTYNISTLPTVFLLDKEGDIVARNVSLPLLEQSIQQLLEK"
     gene            3391..>4383
                     /locus_tag="PLD76_03480"
     CDS             3391..>4383
                     /locus_tag="PLD76_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOU27098.1"
                     /translation="MNTAFRLTRFSLFVLIVLSTMSCRNQQKQFFINEGLVYGTYYRI
                     TYEHYQDIEVDIKEAMAKVGTSLSTFDSLSVISRINRNDSTVVADTYFTEVFTTAQQV
                     STHTGGAFDITVAPLVNAWGFGFDPQHERTAILIDSIKQWVGYNTISLQNGKILKQHP
                     QTQLDASAIAKGYACDVVAQVLREAGIENFLVDIGGELVVKGHNKRNTDWQVGINKAI
                     DDATQQNQEIQQIISVTDVAIATSGNYRQFYYENNKKIAHTIDPRTGYPTNHSLLSTT
                     VIAPTCMLADAYATAFMVMGNWEEIMKVVNNAVPPIAVFCIFEDTTGKHIEMASP"
BASE COUNT         1385 a          909 c          702 g         1387 t
ORIGIN      
        1 cggattcatt attgtattgg tttaccaacc atctcaatgg cttttttcac agtattatct
       61 tgtaaattaa gtattggata aaatgcttca tcaccaagca aattacgagc aatatatgct
      121 tgtagtaaat ttgctaagag agctttcgat ttcgcaatct cttgactatt gcgggaaaca
      181 cctttttgtt ctgcataacg aataaattcg ctaaaataag ttgttttctt tactaagtat
      241 tctactaatt tttcccacgt gtcataagta gataatttag ctcgatgctt atcaacaaaa
      301 gcaaacgaaa aatcatatac aatgccatta tttaaaacgt ttgtgtaata tggtgtataa
      361 taggtagtat catgagggac aaaaatgtcg ggcataatac ctccattagc atatacagtt
      421 cttcctttgg cagtataata cttcaaacta tcagcaaaag aaatactatc tttttggaac
      481 atttcaccat gtaatactcg tttttctatt tctacatcat aattttcatc cgactcgcct
      541 gacttgtacg gtttctgaat agaacgacct gatggtgtgt gatagcgagc cacagtaagc
      601 cttaattcag agccatcgga caacggaatt tgttgttgca ctaaaccctt accaaaagag
      661 cgtctgccaa ttacccatcc cctatcatga tcttgaatgg ctccagcaaa tatttcgcta
      721 gcagaagcag accattcgtt tatcaacaca cagatattct cttttcggaa attacctctt
      781 ccatctgcaa tggcatcttc tctggcttga ttaactccct ctgtatatac aattaattga
      841 cctcttttta aaaattcatt cgtcatctga atgacagcgg ataaataccc ccctgaattg
      901 ttgcgtaaat caacaataat attattgcat tcttgttcac gtaatatgac taatgcatct
      961 aaaaattcac ggtatgtatt agccccaaaa ttactaattt tgacatatcc agtagaatcg
     1021 gctattacgt ataccacatc tatacttttt actggtacat ctcctctcac aatatcaaaa
     1081 tcaagaggca aggggcttcc gttgcgttta atccgtactt taacaatact gcccttttct
     1141 cctctcaatg tttttagcac tgtggcattg gtaacatcag ctcctacaaa actactatca
     1201 ttcactgcaa taatacgatc acctggcaat attcccaatt tttcagaagg accaccagga
     1261 atcacactca caatcataac cgtatcattc tgaatattaa attgcacccc aataccacta
     1321 aaactacctt gtaactcagt agcttctgct acttcttctg gagttaaata agctgaatga
     1381 gggtcgagaa atttcagtat ctgtggtata aactcttctg ttaaagcatt tttatctata
     1441 acatccacat aattattgtc taacaaatac ataatttcat caaacttatt cccttgaacc
     1501 tttttaggaa tagcaaaacc tgtagtttgt tctgcctgtt gataatacaa atttgctaac
     1561 aatattccaa ccgcaatgga tataccaata atgattggat aaagcaattt atgtttttcc
     1621 atacgttaat tatctaaatc aattttgagc acttctaccc cggctctcac gagcaaatca
     1681 accccatcag taacatggta ttcattggca tacacaaccc tcacaatacc cgattgaata
     1741 atcaatttgg cacattctat gcatggagat gtcgttacat ataacgtagc tccttcgcta
     1801 ctattgtgag aacgtgcaat ttttgtaatc gcatttgcct ctgcatgaag cacatagggt
     1861 ttggttttaa aactttcgtc ttcacaaata ttttcaaaac cacacggcgt accattgtaa
     1921 ccatcagaaa taatcatttt atcttttact aatattgctc ctacctgacg gcgtttgcaa
     1981 tacgagtttt cagcccaaat aagagccatt tgcatatatc ttttgtctaa tactaactgt
     2041 tttgtattca tatctcacta tctaaaacga attgatactg ttcgctgttt tatttttcca
     2101 gtaattgttg aatggattgt tccaataaag gtaacgaaac attgcgtgcc actatatctc
     2161 cttccttatc caacaaaaat acagtgggta aggttgatat attatacgtt tgcaacaaag
     2221 gagatactaa tcctttctta tcatgaacaa ctgtataggg caactgtttg gcaacaacag
     2281 aaaatacagc atcatcgtca aacgaaacct gataaatgcg taatcctttg ctggcatata
     2341 cacggtaaag ttcttgtagc aatacagtat gtgcatatga attttcggat tgaaacaacg
     2401 aaaaatccac tatagtggca cgccctaagg atgctgataa agaacgctct accccttgca
     2461 ccgtggacaa ggaaatatca ataaaacctt tgccaactat ttctctaact agtttatctt
     2521 ctgcattttt tcgtcgttgc atacctttta gcaccacgtc ttcgatattc tttgtacgtg
     2581 ccgtttgtgg ataatgcact tgcaagggtg tagccactgc agcatacact tttacatctt
     2641 ctttatcata aggattaaac acataatacc cattaatctg ctgatacaaa gcgaaataag
     2701 ccacacttga tcgcgtatct tgatatacct ctttgcgtaa gaaatctttt agttgacgaa
     2761 caagctgcgt cgtttcttct gtaaaatctt gtgccactat tttgccacgt tgatacttat
     2821 cttgactagc tgtcatctta cgttgtgtct gtgcaacata gtgcacaatg gattgcatgc
     2881 gtttagatgc tactgaccca actatgctat ctgcttgacc aaatgtttgt ttgtttgaat
     2941 agacagaaat cgtttcggta gagtcggcta ctaaatacaa tacaccttgt tgcaaacgca
     3001 aacgcacata gtgaggttca actgcacgag gaaaggaaaa agaaaacttg ccattttttt
     3061 taagtacttg ataagcgact atctctattt ttcccaactc aactttttcc aaaaataaag
     3121 tgtctgtttc aactcccgac actgtgcctt gaatggtata ttcttccgtt tgagagcatg
     3181 atgacaataa acaaacaaac accacgaata aaaataagat tgattgcttc atactgctca
     3241 tactggtata acattacatg taccgaatac cctataataa ttgtgtaaat aaaccttgca
     3301 aaaatacaac atctttcagc taaaatcaaa aaaatcgtat ctttgtgtgc ttaaaatgag
     3361 tattttattg tctattaaca catattgcta atgaataccg cattccgatt aacacgtttt
     3421 tccctttttg ttcttattgt actttctaca atgagttgta gaaaccaaca aaaacaattt
     3481 tttataaacg aaggtttagt atatggtacc tactaccgca tcacctacga gcactatcaa
     3541 gatatagaag ttgacataaa agaagctatg gcgaaagtag gaacatcttt atctacattt
     3601 gacagccttt ctgtaatttc tcggattaac agaaatgata gcaccgtagt ggctgacact
     3661 tattttacgg aagtatttac gacggctcaa caggtttcaa cacatacggg aggtgctttt
     3721 gacataacag ttgcaccatt ggtaaacgca tggggatttg gttttgaccc tcaacatgaa
     3781 agaacagcca tcctaataga tagcatcaaa caatgggttg gatacaacac aatatcctta
     3841 caaaacggca aaatcctgaa acaacaccca caaacgcaac ttgacgccag tgctatagcc
     3901 aaaggatatg cgtgcgatgt agtagctcag gtattacgtg aggctggtat cgaaaatttt
     3961 ttggtggata ttggtggcga attggtagta aaaggacata ataagagaaa tactgattgg
     4021 caagtaggta tcaacaaggc aattgatgat gctacgcaac aaaaccaaga aatacaacaa
     4081 attatttcgg taacagatgt tgccattgct acttcgggta attatcgtca gttttactac
     4141 gaaaacaaca aaaaaattgc ccataccatc gacccccgta ctggctaccc taccaatcat
     4201 tcgctgttga gcaccaccgt aattgctcct acctgtatgc tagctgatgc gtatgctacc
     4261 gcctttatgg taatgggaaa ttgggaagaa ataatgaaag tagtaaacaa tgctgttcct
     4321 cctattgctg ttttttgtat ttttgaagat actacaggca aacatatcga aatggcttct
     4381 cct
//
LOCUS       DAOSMY010000109         4346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20247_length_4346_cov_2.851317, whole genome shotgun sequence.
ACCESSION   DAOSMY010000109 DAOSMY010000000
VERSION     DAOSMY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20247_length_4346_cov_2.851317"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLD76_03485"
     CDS             complement(<1..303)
                     /locus_tag="PLD76_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBM20 domain-containing protein"
                     /protein_id="HOU27099.1"
                     /translation="MKLTFTIQYHTSWGQQVCILGSLPALGNNIPTKALKMNYIESGQ
                     WEASIQVRSLASFSYTYVVVDANGHTISEWGNPREIEIPASYKNAIIQDNWRSNGTD"
     gene            complement(435..2414)
                     /locus_tag="PLD76_03490"
     CDS             complement(435..2414)
                     /locus_tag="PLD76_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU27100.1"
                     /translation="MQNTTNTFDYHVDKFADIEILRYRVTGFEALSLQQKQLIYHLSE
                     AAQEGRDILFDQHYEHNLCIRRTLEAIYETHNETEKNVEEFNAFEIYLKRIWFSNGIH
                     HHYSTDKLEITCSKEYFTSLLKKVPVSLLPLEKNETIDSFYTKIITLIYNPEIDKKRV
                     NLADNQDLITSSANHFYKNVTQQEAEDFYNGLQDSSDLQPISYGLNSCLVKENGIIKE
                     KTWKLNGMYTAAIEKIIYHLSNAMAFAENSKQKLIIEKLIAFYQTGNLQTFDEYSILW
                     VKDTSSEIDFLNGFIETYGDPLGRKGSWEAIVNFINKEATQRTQTISNHAQWFEKHSP
                     VNERFKKTSVVGVSAKVITNAMLGGDSYPATPIGINLPNSNWIREVHGSKSVTIDNMM
                     EAYHHAASGNGFNEEFMWSQHECELTEKYGYITDKLHTDLHECVGHGSGKMLDGVSPE
                     ALKAYGATIEETRADLFGLYFIADPMMVEIGLLPNMDAYKAEYYRFMLNGLMTQLVRI
                     KIGDTIEESHMRNRQLIANWVFEKGQKQKCIEKRVQNNKTYIIIHDYVALRTLFGELL
                     AEIQRIKSEGDYTAAKYLVEKYAIQIDYSLHKEILERYTALDIAPYKGFVNPTYEQVY
                     NEYGEVIDIVPHYTESYTEQMLRYSKTKSYLPTYN"
     gene            complement(2422..3243)
                     /locus_tag="PLD76_03495"
     CDS             complement(2422..3243)
                     /locus_tag="PLD76_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27101.1"
                     /translation="MKKIALLILFTLLCVSHVVYAQTIQENEQMIVSWFEDIKETQSD
                     SAKIQLNKKIEQLLYTTLQEPNAFNYSFPALKYIGSIYAPDKLMRVFSWNYPLSNGKY
                     GYSALFMFKNKPKSTPSLHLVSTTTAFKPEETRKYDPSTWYGALYYNVIAHKISRDKT
                     EYLLLGISNYQPITKVKIIESIKISNRQLTFGTPLFKKDKKTLYRAVFEYKKEVNMLL
                     EYESSKKRFVFDHLSPSDPIYKDVYHFYGPDFSYDSYQWKKKKLHYTPDIDVRNK"
     gene            complement(3316..>4346)
                     /locus_tag="PLD76_03500"
     CDS             complement(3316..>4346)
                     /locus_tag="PLD76_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HOU27102.1"
                     /translation="IINPPYPTMTEKEIDQTYTLPYTRLPHPRYKGKTIPAYEMIKHS
                     VTLHRGCFGGCAFCTISAHQGKFIASRSEKSILAEIEKITQMPDFKGYISDIGGPSAN
                     MYKMQGIDLTICEKCKKPSCIHPKICKNLDTNHAPLIELYKKVRSIKEIKKAFVGSGI
                     RYDMILHKTGNKQTDASNMEYANELIAHHVSGRLKVAPEHTNDEVLNLMRKPSFEYFE
                     TFVKLFNEVNKQHHLNQQLIPYFISSHPGCKQQHMAELAAKTKALDFKLEQVQDFTPT
                     PMTLATEMYYTGIHPYTLEKISTPNTPQQKLNQRKFFFWYQKEYKNEIINDLKKMNRM
                     DLIKKLYP"
BASE COUNT         1201 a          735 c          795 g         1615 t
ORIGIN      
        1 atcggtgcca ttcgaacgcc aattgtcttg aatgattgca tttttatatg aagctggtat
       61 ttctatttct ctgggatttc cccattctga tattgtgtgt ccatttgcat ctactaccac
      121 atatgtgtaa gaaaaagagg caagcgagcg aacttgtatg cttgcttccc attgtccact
      181 ttctatgtag ttcattttta atgctttagt tggaatattg tttcccaaag ctggtagtga
      241 tccaagtatg cacacttgct gtccccacga agtgtggtac tgaattgtaa aggttagctt
      301 catagttttt ttcaaaaaat agttaggaat agtttaacgt aaaacgaaga caaatgtaat
      361 acgtttgtaa tccccaaagc tagtatagag ctacaaaaat gcataaaaaa ccttgcaaac
      421 gtttgatagg tattttagtt atatgttggt aggtaagatt tagtttttga ataccgcaac
      481 atttgttctg tataactttc agtataatga ggtacgatat ctattacttc tccatactcg
      541 ttatacacct gttcgtaagt gggatttaca aatcctttat aaggtgcaat atccagagca
      601 gtataacgtt ctagaatttc tttgtgcaaa gagtaatcaa tttggatagc gtatttttct
      661 actaagtatt tggctgctgt gtaatcgccc tcggatttta tgcgttgaat ttcggcaagt
      721 agttcgccaa acaatgtgcg taaagctaca taatcgtgta tgatgatata ggttttatta
      781 ttttgcactc ttttttcgat gcatttttgt ttctgtcctt tttcgaaaac ccaattagca
      841 atgagttgtc ggttgcgcat gtgcgattct tctattgtgt caccgatctt tatacgtacc
      901 agttgagtca ttaatccatt aagcataaat cggtagtatt cagctttgta tgcgtccata
      961 tttggtaaca aaccaatttc taccatcata gggtctgcaa taaaatacaa cccaaataaa
     1021 tcggctcggg tttcttctat cgttgctccg tatgctttta acgcttctgg agaaacccca
     1081 tcgagcattt tacctgaccc atgtcctaca cattcgtgca ggtcggtatg taatttgtcg
     1141 gttatatagc catatttttc ggtcaattca cattcgtgtt ggctccacat aaattcttcg
     1201 ttaaagccat taccactagc agcgtgatga taagcttcca tcatattgtc gatggttact
     1261 gattttgatc catgtacttc acgtatccag ttagagtttg gcaagttaat gccaataggg
     1321 gttgcaggat aactgtcgcc accaagcatg gcattggtaa taacctttgc cgatacgcct
     1381 acaacggatg ttttcttaaa tctctcattt actggagaat gtttttcaaa ccattgagca
     1441 tgattactta tggtttgggt gcgttgggtt gcctctttgt tgataaaatt gacaatcgcc
     1501 tcccagcttc cttttcgccc aagcgggtcg ccataggttt caataaaacc atttaaaaaa
     1561 tcaatttcgg atgaggtgtc ttttacccaa agaatggaat attcatcaaa agtttgtaag
     1621 ttgcccgttt gataaaacgc tattaatttc tcgatgatga gtttttgctt gctattttca
     1681 gcaaatgcca tagcattgga taaatggtat ataatttttt caatagcagc cgtgtacatg
     1741 ccattaagtt tccatgtttt ttctttgatt atgccatttt cttttaccaa acaactgttc
     1801 aaaccataac ttattggttg caaatcgcta ctatcttgta acccattata gaaatcttca
     1861 gcctcttgtt gtgttacatt tttgtaaaaa tggttagcag aacttgtaat taaatcctga
     1921 ttatcagcga gattcaccct tttcttatca atctctgggt tataaattaa ggtaattatt
     1981 ttggtgtaaa aactatctat tgtttcattt ttttctagtg gaagaaggct tacaggaact
     2041 ttttttagta gtgaagtaaa atattcttta gaacacgtaa tttcgagttt atcggtagaa
     2101 taatggtggt gaataccgtt agaaaaccat atgcgtttca ggtaaatttc aaaagcgttg
     2161 aattcttcta cattcttttc tgtttcatta tgtgtttcgt atatggcttc gagtgtgcgt
     2221 cgaatacaca agttgtgttc ataatgttga tcgaacaaga tatctcgccc ttcttgtgca
     2281 gcttctgaaa gatgataaat taattgtttt tgttgcaatg acaaggcttc aaagccagta
     2341 acacgatatc tcaatatttc aatatcggca aatttatcta cgtgataatc aaatgtattt
     2401 gttgtatttt gcatagttgt tctatttatt tcttacatca atatctggtg tgtagtgaag
     2461 ttttttcttt ttccattgat aggagtcata cgaaaaatca ggaccataaa aatgatacac
     2521 atctttgtat atagggtcag atggtgaaag atggtcgaat acaaatcgtt ttttgcttga
     2581 ctcatattct aataacatgt ttacctcctt tttatattca aaaacggctc gatagagtgt
     2641 ctttttatct tttttaaaaa ggggagtgcc aaatgttagt tgccgattac taatttttat
     2701 agattcaatt atttttactt ttgtaatggg ttggtaattg ctgatgccca gtaataggta
     2761 ttctgtttta tctcggctaa ttttgtgtgc tattacgttg taatacaatg ctccatacca
     2821 cgttgaaggg tcgtatttgc gtgtttcttc aggtttaaaa gctgtagttg tcgatacaag
     2881 gtgcaacgag ggtgtgcttt ttggcttgtt tttgaacata aatagtgccg aatagccata
     2941 ttttccattg cttaatggat aattccaaga aaaaacacgc attagtttgt caggagcata
     3001 tatacttcca atgtatttta aggcaggaaa tgaataatta aacgcatttg gctcttgcaa
     3061 tgttgtgtaa agcaattgtt caatcttttt attgagttgt atctttgcac tatcagattg
     3121 agtctctttt atatcttcaa accacgaaac aatcatttgt tcgttttctt gtattgtttg
     3181 tgcatatact acatggctta cacataacaa ggtgaaaaga ataagtagtg cgattttttt
     3241 cattggattt ctattagctt acgctactgc tacacagttt taaatattgg taatattatg
     3301 atttactggg ctatactatg gatataattt tttaattaag tccattctgt tcatcttttt
     3361 taaatcgttg ataatctcat ttttatattc tttttgatac caaaagaaaa actttcgttg
     3421 attgagtttt tgctgaggtg tgtttggtgt agaaattttc tccaaggtat atggatgtat
     3481 acccgtataa tacatttccg ttgctagtgt catcggagtt ggtgtaaagt cttgtacctg
     3541 ctctaatttg aaatccaatg ctttggtttt ggcagctaat tcagccatgt gctgttgttt
     3601 acaccctgga tgactcgaga taaagtaggg tataagctgt tgatttaaat ggtgttgctt
     3661 gttcacttcg ttgaatagct taacgaatgt ttcaaaatac tcaaaagatg gttttcgcat
     3721 caagttaagg acttcatcgt tggtatgctc aggagctact tttagtcgac cagatacgtg
     3781 gtgtgcaatt aattcgttcg cgtactccat attggatgcg tctgtttgct tattacccgt
     3841 tttatgcaat atcatatcat agcgaatacc actaccaaca aatgcttttt ttatttcttt
     3901 aatgctgcgt acttttttat acaattcaat caatggagca tgattagtat ccaaattttt
     3961 acaaattttt ggatgaatac acgatggctt tttgcacttt tcgcaaattg ttaaatcaat
     4021 accttgcatt ttatacatat tcgctgatgg acctccaata tcgctaatat atcctttaaa
     4081 atcaggcatt tgagttatct tttcaatttc tgctaaaatg gatttttctg aacgtgatgc
     4141 aataaatttg ccctgatgtg cagaaatagt acaaaaagca caaccaccaa aacatcctct
     4201 gtgtaacgta acagagtgtt ttatcatttc gtatgcagga atggtttttc ctttgtaccg
     4261 tggatgtggc aagcgtgtgt atggcaaagt gtaggtttgg tcaatttctt tctccgtcat
     4321 cgtcggataa ggtggattaa taatca
//
LOCUS       DAOSMY010000110         4314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20452_length_4314_cov_4.249589, whole genome shotgun sequence.
ACCESSION   DAOSMY010000110 DAOSMY010000000
VERSION     DAOSMY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20452_length_4314_cov_4.249589"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..184)
                     /locus_tag="PLD76_03505"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="PLD76_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(407..1246)
                     /locus_tag="PLD76_03510"
     CDS             complement(407..1246)
                     /locus_tag="PLD76_03510"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOU27103.1"
                     /translation="MKKLFVYILLFVLPIGNIHSQETNSCGITNTSFKAGEKLRYSAY
                     FRWGILQLSSFSIQSEVKRERYKGESVFAITTTGVTAGNMDKFFTIRDTLHAKLKVND
                     LQPLYFKEIANEEKFKATREFKYDYTDSTRVSWQFADNKNKEKAGNTTLTNCAYDAVS
                     IIYRLRNYDFNAETLKPNQTFRIDYFSDGKSSPLQMRFEKKEIVTLKNGDRYDCLKFE
                     LEVAEGTLFKNNERVFIWITNDKNRLIAYVEAKLKIGYVKMDLVNVEHHRHPLAAKLK
                     PVK"
     gene            complement(1250..3052)
                     /locus_tag="PLD76_03515"
     CDS             complement(1250..3052)
                     /locus_tag="PLD76_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU27104.1"
                     /translation="MISTLIKRILLISAITVSVTLLLHSCANRGAGPQGGIRDTTPPQ
                     VLKSSQSNKATSATNQRLEWIFDENIQLEKQQENILVSPPQKQAPTIRAMGKKLSVAF
                     ADSLTSNTTYTIDFGNAVVDLNEKNALANFSWNFATGATIDTMQLAGYVIDARTLTPY
                     EGIIVGVYKSNKDSAFVSTPFDYVARTNSQGFFTIRNIPQVPYQIYALKDIPQAFYYD
                     QKGKEIGFFSQKVTPEVVKHLQTDTIWNENDTVSILRIDTSYSISYAPNDVLIKLFSE
                     ERAMIPTLQRALRPSKESFTLLMSAPLDKEPLITPINVETSNWLKREQTNRMDSLVYW
                     ITDSNALALDTIQFTVSYASADSLKTDTIALTYKQPRARTANEKTQQQLEIVSLTKSP
                     VDIADTLRLQVKEPMKSLNIEKMHLTMRIDTTWHNVPFSLQYDDDNCKKKISVLFDRK
                     ERANYRLVIDSAAIVSDYGKQNTSFVQTFTTKSPDDYASLMIEWKNIPPYGIVELLDA
                     KETVVRAVHVSEPTTVLRLLSPGNYFLRLVLDANQNGKWDTGSVLPKLEPEEVYYYPK
                     RLVLRANWDSVEEWDYTAIPLLQQRATELPKSKK"
     gene            complement(3049..>4314)
                     /locus_tag="PLD76_03520"
     CDS             complement(3049..>4314)
                     /locus_tag="PLD76_03520"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOU27105.1"
                     /translation="LHHIWKYRLFASHQLTDTEGSTIDIIDVGHHNLDAGPDFINAKI
                     KIGDTLWAGNVEIHKKASDWNTHKHPTNPAYNSVILHVVAECNTDIFTQEGRKIPQLK
                     LPLTPALLEQWQQLEHSPHKIACHLHLHRISDMIKTSWLEALVIERLETKASRIDTLL
                     EQNKNNWEETFYQTLARNFGFGLNSDPFEWLAKSLPINYIAKHKNQLLQIEALLFGQS
                     GLLQLSQATDDYTVQLKNEYAFLQQKFSLTPIDGSLFKMMRLRPANFPHIRLAQLAQL
                     IHTSSKLFSHIVENPTYSSLKKMFAVSTSLYWETHYTFGEVSPRKTKKLGEKAINSII
                     INTIVPILFAYAKRKNNDELQEKSVHLLESCAAEDNSIVRNWQEMGFTATSAYQTQAL
                     IQLQKQYCQTKKCLYCRFGHKLLQLSLQP"
BASE COUNT         1074 a          805 c          893 g         1542 t
ORIGIN      
        1 taatatgtgt atatatctca gttgttttac tactcgaatg acccaacaaa ctttgaatat
       61 atcttaagtc tgtcccattt tctccttcaa acaaccactc tttgggtttg tattctttaa
      121 aatattttcg taaaactact aaagtttttt taggtagcaa agtgtatcgg tctttttttc
      181 ctttgctttg ttgtattttt atcccgtttg agcgggatgt ccgctatact gccatttgca
      241 tacggtcgct acactatctg ttatttagat tagtagattt attgtaatac atcattatcc
      301 caatgtgcag ggttataggt catgttttgg ttttttaatt cgcaaaccgt atccgtgtct
      361 tttccctttg ctttgcgtaa tttccttttc aatgggcagg ctatccctac ttcacaggct
      421 tcaacttagc agctaatggg tgtcggtggt gttctacgtt taccaagtcc atttttacgt
      481 agccgatttt tagtttggct tctacgtagg cgatgagtct gtttttgtcg ttagtaatcc
      541 agataaatac gcgttcgttg tttttgaaga gggtgccttc ggctacttct agttcaaatt
      601 ttaggcagtc gtatctatcg ccatttttga gggtaactat ttcttttttc tcaaaacgca
      661 tttgcaaggg cgaagatttg ccatcgctaa aatagtcgat acgaaatgtt tggtttggtt
      721 tgagcgtttc tgcattgaaa tcataattgc gtaagcgata aataatgctt actgcatcat
      781 atgcacaatt tgttagcgtt gtgtttcctg ctttttcctt gtttttattg tcagcaaatt
      841 gccagcttac ccgcgttgag tcggtgtaat catacttaaa ttctcgtgtg gctttaaatt
      901 tttcttcgtt tgcaatttcc ttaaaataaa gcggttgtag gtcgtttact tttaatttgg
      961 catgtaaggt atcgcgtata gtaaaaaatt tatccatatt gcccgctgtt acgcccgttg
     1021 tggtaatggc aaataccgat tctcctttat atcgttctct ttttacttcg ctttggatgc
     1081 taaaactgct caattgtagt attccccaac gaaaataggc actatacctt agcttttcgc
     1141 ctgctttaaa acttgtatta gttatcccac acgagtttgt ttcttgtgag tgtatattcc
     1201 caataggcaa aacgaatagc agtatatata cgaatagttt cttcatggct tattttttgc
     1261 tttttggtaa ttcagtagct cgttgttgca acaagggtat ggcggtataa tcccattctt
     1321 ctacactgtc ccagttagct cgcaatacca aacgcttagg gtagtaatac acttcttcgg
     1381 gttctagctt gggcaatacg cttcccgtat cccatttccc gttttgatta gcatcgagta
     1441 ccaaacgcaa aaaatagtta cctggactaa gaaggcgaag tacggttgtt ggttcgctta
     1501 cgtgtactgc acgtacaact gtttctttgg catctagcaa ctccactatg ccataaggtg
     1561 gtatattctt ccactcaatc attaacgaag cgtaatcatc gggcgatttg gttgtaaatg
     1621 tttgtacaaa cgaggtgttt tgtttgccat aatcacttac tatagctgcc gagtctatta
     1681 ccaagcgata gttcgctctt tcctttctgt cgaacaatac actgattttt ttcttgcagt
     1741 tatcgtcgtc atattgcaat gaaaaaggaa cattgtgcca cgtagtatct atacgcatgg
     1801 tgagatgcat tttttcaata ttaagcgatt tcatcggttc ttttacctgc agacggagag
     1861 tgtctgcaat atcgactggc gattttgtta gcgacacaat ttcaagctgt tgttgggttt
     1921 tctcgttagc agttcttgct cgtggctgtt tgtatgttaa tgctattgtg tccgttttta
     1981 ggctatctgc actggcatac gatactgtaa attggatagt atccagcgct aaggcgttgg
     2041 agtcagtaat ccaatacacc agagagtcca tgcggtttgt ctgttctcgt ttcaaccaat
     2101 tgctggtttc tacgttgata ggggtaatca gcggttcttt gtcgagaggt gcggacataa
     2161 gtagagtaaa cgactctttg ctggggcgga gagccctttg taaagtagga atcattgctc
     2221 gctcctcgct aaagagtttg ataagcacat cattgggtgc atacgaaata gagtaagacg
     2281 tgtcaattcg caagatagaa acagtatcgt tttcgttcca aatagtatct gtttgcaggt
     2341 gttttaccac ttcgggggta actttttggc tgaaaaaacc tatttctttt cctttttggt
     2401 cgtaataaaa tgcttgtggt atatctttta gggcatatat ttgataaggc acttggggaa
     2461 tgtttcggat tgtaaaaaac ccttggctat tggtgcgagc cacataatca aacggagtag
     2521 aaacaaacgc actatcctta ttgcttttat atacccctac aatgatgcct tcgtacggag
     2581 taagcgtgcg tgcatctatt acataaccag cgagttgcat ggtatcaatg gttgctcctg
     2641 tggcaaaatt ccacgagaag ttggcaagtg catttttttc gttcaaatcg accacagcat
     2701 tcccaaaatc aatggtatag gtggtgttcg aagtaagact atccgcgaaa gcaacgctca
     2761 gttttttacc cattgctcgt atggttgggg cttgtttttg tggaggtgaa acaaggatat
     2821 tttcttgttg tttttcaagc tgaatatttt cgtcaaaaat ccattctaat cgttggtttg
     2881 ttgccgaagt agctttgttt gactggctac tttttagcac ttgtgggggt gttgtgtcgc
     2941 gtatgccacc ttgtggtcct gcacctctat tggcacacga atgcaatagc aaggttacac
     3001 ttacggtgat ggctgaaatg agtaaaatgc gtttaatcag ggttgatatc atggttgtaa
     3061 acttaattgt aataatttat gtccaaatcg acaatacaaa catttttttg tctgacaata
     3121 ctgtttttgt agctgtataa gggcttgtgt ttgataggct gaagtggctg taaaacccat
     3181 ttcttgccaa tttcgcacaa tactattgtc ttctgcagca caactttcga gcaaatgtac
     3241 ggatttttct tgtaattcat cattattttt cctttttgca tacgcaaata atatggggac
     3301 aatggtgttg atgatgatgc tgttgatggc tttttctcct agcttttttg tttttcgtgg
     3361 cgaaacttcg ccaaaggtat aatgtgtttc ccaatacagt gatgtgctta ccgcaaacat
     3421 cttttttagg ctgctataag tagggttttc tactatgtgc gaaaaaagtt ttgacgatgt
     3481 gtgtatcaat tgtgcaagtt gagccaaccg aatgtgcgga aaattagctg gtcgcaatcg
     3541 catcatctta aataaactgc catcgatagg tgtaagcgaa aatttttgtt gcaaaaaggc
     3601 atattcattc ttcaattgca cagtataatc atctgtggct tggcttaact gtaataaacc
     3661 cgattgtcca aaaagcaagg cttctatttg taaaagttgg tttttgtgtt ttgctatgta
     3721 gtttattggt aacgatttag ctaaccattc aaatgggtcg ctgtttaatc caaatccaaa
     3781 attgcgtgca agtgtttgat aaaaagtctc ttcccaattg tttttgtttt gttctagtaa
     3841 ggtgtcaatg cgtgatgctt tggtttccaa acgctctata acgagagcct ctagccaact
     3901 agtttttatc atatcactga tgcgatgtag gtgcaaatga caagctattt tgtgcggact
     3961 gtgttccagc tgttgccatt gctccaacag agcaggggta agtggcagtt ttagttgggg
     4021 tatttttctc ccttcttgtg taaaaatatc tgtattacat tctgcaacta catgcagtat
     4081 aacagaatta tatgctggat ttgttgggtg tttgtgtgta ttccaatcgg acgctttttt
     4141 atgaatttct acattgcccg cccaaagggt atcgccaatc ttaatttttg cgttgataaa
     4201 atccggacca gcatccaagt tgtgatgccc tacatcaatt atatctatcg ttgacccttc
     4261 tgtgtcggta agttgatggc tagcaaataa ccgatatttc cagatatgat gcaa
//
LOCUS       DAOSMY010000111         4303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20511_length_4303_cov_3.860169, whole genome shotgun sequence.
ACCESSION   DAOSMY010000111 DAOSMY010000000
VERSION     DAOSMY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20511_length_4303_cov_3.860169"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1109)
                     /locus_tag="PLD76_03525"
     CDS             complement(249..1109)
                     /locus_tag="PLD76_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU27106.1"
                     /translation="MRQLKIVKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIK
                     KGDRIALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPSPEELAE
                     ELDIPVDKITDTLKVSGRHISVDAPFVEGEDNSLLDVLVNDDSPVADRFLLNESLATE
                     IDRALNTLSEREKDIIVSFFGISCQEMTLEEIGDKFGLTRERVRQIKEKAIRKLRSSS
                     KSKLLKGYLG"
     gene            1292..1652
                     /gene="rnpB"
                     /locus_tag="PLD76_03530"
     ncRNA           1292..1652
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD76_03530"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1671..3011
                     /locus_tag="PLD76_03535"
     CDS             1671..3011
                     /locus_tag="PLD76_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOU27107.1"
                     /translation="MARFTTLIIFFKSALHFLCFDIWRITDGEVTAAKNRIYTIIKVL
                     VISVRRFFEDKIFAKSAALTYYTLLSVVPVLALVIAIGKGFGVQYYLENQFVAIFPSH
                     STVLKEAFFLVDSYIQQIQGGVFFGIGFVLLFWSVLNMFSEIEIAFNDIWQIKKLRNT
                     FRRFADYFSMMLILPVLLIVSSGLSIYLNGTIQDTVLSSLIVPVWYGFLKILPFIINI
                     VLFTLMYLFFPNTKVRFIAALFAGVVAGVLFQLFQMLYISGQIWISYYNAIYGSFAAL
                     PLLLLWLWASWSIILYGAEFAFSVQNIKNYEFESDVKNISRRYENFLFVLISSVIVKR
                     FAEKLPAMNAEELSTHYNIPIRLVNRIVSKLLDAGIIVESISTVKKTEEIVYQPAIDI
                     QHLTLAYLFEQIDGLGSENFKIDITHEHAQPWQATLFVQNSTQSYASSVLLKDL"
     gene            complement(3018..4055)
                     /gene="mltG"
                     /locus_tag="PLD76_03540"
     CDS             complement(3018..4055)
                     /gene="mltG"
                     /locus_tag="PLD76_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOU27108.1"
                     /translation="MNYLRNHKTLLILVAVIGITASGLMLNAFILPNLNGNGNKKQYL
                     FVYPEQTWSDVINTLTEMEVVNSTFSLKLAIALKYADMPPKTGKYDIEPNLSNRYLLN
                     RIAHGLQSKIILRVVSTRNTQQVARNLSRQLMVDSTSLMKQFHNPTLLKKYELTPTNS
                     LCLFLPFNAEITWDTPIEEIIQLIEKEYTTFWNDNRKQLAKKINLTPSEIHILASIVE
                     EETNNFEDKKMVAGLYINRLRKDMLLQADPTVRYATGNFSLNRILKRHLAINSPFNTY
                     IYKGLPPAPIRIPTQESIDAVLNYTKHPYVYMVAKSDFSGTHNFSRTFDEHLRKARAY
                     QKQLNKRGIFE"
     gene            4174..4260
                     /locus_tag="PLD76_03545"
     tRNA            4174..4260
                     /locus_tag="PLD76_03545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4208..4210,aa:Ser,seq:gct)
BASE COUNT         1248 a          689 c          796 g         1570 t
ORIGIN      
        1 ttttcaccat gtttttagta atactacttt gtacgaacta tacatggtag gcaaagttaa
       61 aaaaagaaaa ttaagaaaag aatattttaa ggaaaataat acaacaatag gaagaattaa
      121 tgctcatcat taggtgttta attgttgagt ttagtaaatt gaatagtttt cattgtgttc
      181 ctatatacaa aaaaaaagac catcatacaa aaaaagatgg tcttaggtta tgtttgagag
      241 ctaggtgatt aacctaaata cccttttaat agtttacttt tgctactact acgtaacttg
      301 cgaatggctt tttcttttat ttgacgtaca cgttcacgtg ttaagccaaa tttatctcca
      361 atttcctcta atgtcatttc ttgacacgaa ataccaaaaa acgatacaat gatatctttc
      421 tctctttcgc ttaatgtgtt taatgctcta tcaatttcag ttgccaacga ttcgttaagc
      481 aaaaacctgt cagccacggg cgaatcgtca ttaaccaata cgtcaagcaa actattgtct
      541 tcaccttcta caaagggagc atctaccgaa atatgacgac cagatacttt cagcgtatcc
      601 gtaattttat ctacaggtat atccaactct tcagccaatt cctctggcga aggacgacgt
      661 tcattttctt gttcaaattt ggaaaaagct ttgttgattt tatttaatga tcctacttga
      721 tttagtggta aacgtacaat acgagactgt tctgctaagg cttgtaatat tgattgacga
      781 atccaccata cagcgtacga aatgaactta aaaccacggg tttcatcaaa tttttcagca
      841 gctttgatta atcctaaatt tccttcattg attaaatctg gtaggctaag accttgattt
      901 tgatattgtt ttgctacaga tacaacaaat cgtaaattgg ctctagtaag tttttcgagg
      961 gcaattctat ctcctttttt tatgcgttga gctagctcaa cttcttcttc tactgtaata
     1021 aggtcttcgc gtccaatttc ttgtaaatat ttgtccagcg aagcactttc tctattagtg
     1081 attgatttta cgattttcag ttgtctcata atgcgggcaa aattagtttg caaagataga
     1141 agaaaaaaac gaattaacga aaaaatagtg tactcattct tcttctatat gttattttat
     1201 gttatctcat ttagtttttc tatcccaatt ttctatttca ttttcattaa agtgttgttt
     1261 caataacata attgcgtatc tttgtattca gatagtaggt cggtttgtcg ccgttcattg
     1321 tgtgtgaaag gaggaaagtc cgggcaacgt agagcaccat acttcttaac gggaagttgt
     1381 tcgtgagagc aaagtagtgt aacagaaaat aaccgccatg ccttgtgtgt ggtaagggtg
     1441 aaaaggtgag gtaagagctc accggtttgt ttggtaacaa gcagagccgt acgccttatg
     1501 ggttgcaaga ccatgtatac cgacgcatgt agggctgctc gtccgatgtc ggggggtagg
     1561 ttgctagagg caacaagtga ttgttgttgt agataaatga caaacattta cgcaagtaaa
     1621 tacagaaccc ggcttataga cctgctattt ttttataata cttgtatgct atggctagat
     1681 ttactacatt gattattttt tttaaatcag ccttacattt tttatgtttc gacatttggc
     1741 ggataacgga tggggaagtt acggcagcta aaaatcgtat ttataccatt ataaaagtat
     1801 tagttatttc tgttcgtcgt ttttttgaag ataaaatatt tgcaaagtcg gcggctctga
     1861 cctattatac cttgctttct gttgttcctg tacttgcctt agtaattgcg attggtaagg
     1921 gctttggtgt gcaatattat cttgagaatc aattcgttgc gatatttcca agccatagta
     1981 ctgttttgaa agaagctttt ttcttagtcg attcatatat tcagcaaatt cagggtggtg
     2041 tgttttttgg aattggtttt gtattgttgt tttggtctgt acttaacatg ttttccgaaa
     2101 ttgagattgc tttcaatgat atttggcaga taaaaaaatt gcgaaatact tttcgtcgtt
     2161 ttgccgatta tttttccatg atgttaatac ttcctgtgtt actcattgta tctagtggat
     2221 tgtcaattta tttgaacggg actattcaag atacggtgct ttcttcgttg attgtgcctg
     2281 tatggtatgg ttttctcaaa atattacctt tcattattaa tattgttttg tttacattaa
     2341 tgtatttatt ttttccaaat acaaaagtga ggtttattgc cgctttattt gccggagtag
     2401 tcgctggtgt attgtttcag ttattccaaa tgttgtatat tagcggacaa atttggatat
     2461 cttattataa tgccatttac ggtagttttg cagcattgcc attgcttttg ttatggttat
     2521 gggcgtcgtg gtctatcatt ttatatggtg cagaatttgc tttttctgtg caaaatatta
     2581 aaaattacga atttgagtct gatgttaaaa atatatctag gcgttacgaa aactttttgt
     2641 ttgttcttat cagttctgtc attgttaagc gttttgccga aaagcttcct gcaatgaatg
     2701 cagaagaatt atctactcac tataatattc caatacgatt agtcaatcgc attgtatcta
     2761 agttactaga tgcagggatt attgttgaga gtatttctac tgtaaaaaaa acggaggaaa
     2821 ttgtttatca gccagctata gatatccaac atttaacgct tgcttattta tttgagcaaa
     2881 tagatggatt ggggtctgag aattttaaaa ttgacattac acatgagcat gcccaacctt
     2941 ggcaagctac cttatttgtt caaaatagca cacagtccta tgcttcttct gtattattaa
     3001 aagatttata acagacttta ttcaaaaata ccacgtttgt tcaattgctt ttggtatgct
     3061 ctagctttcc gcaaatgttc gtcgaatgtg cgactaaagt tgtgtgtacc agaaaaatca
     3121 ctttttgcga ccatatatac atatgggtgt ttggtgtaat tgagtacagc atcaatactt
     3181 tcttgggtag gaatacgaat gggagcagga ggtagtcctt tatagatata tgtattgaat
     3241 ggagagttga tagcaaggtg tcgttttagt atccgattaa gagaaaaatt gcccgtagca
     3301 tatcgcacag tagggtctgc ttgtaacaac atgtcttttc gtaatctatt gatatagaga
     3361 cctgctacca ttttcttatc ttcaaaatta tttgtctctt cttctactat agaagctaaa
     3421 atgtgaatct cagatggtgt taaatttatt ttcttggcta attgttttct gttatcattc
     3481 caaaacgttg tatattcttt ttctatcaat tgtataattt cttcgattgg cgtatcccat
     3541 gtaatttctg cattaaaagg taagaacaaa cacaatgaat tagtcggagt aagttcgtat
     3601 ttttttaata aagtaggatt gtgaaattgt ttcattaacg aagttgagtc aaccattaat
     3661 tggcgactaa gattacgtgc aacttgttga gtattacggg tagaaaccac cctaagaata
     3721 attttgcttt gtaatccgtg agcaattcta ttcagtagat acctgttaga tagattgggc
     3781 tctatgtcat attttcctgt ttttgggggc atatcagcat atttcagagc aatcgcaagt
     3841 ttcaaagaaa atgtactatt aacaacctcc atttctgtta acgtgtttat tacatcactc
     3901 cacgtttgtt caggatacac aaagagatac tgttttttgt taccattacc atttaaatta
     3961 gggagaataa aggcattaag cattaaacca ctagcagtta ttcctattac tgctactagt
     4021 ataagtagtg ttttatggtt acgtaaatag ttcattttct tttttgtttt ttacaaatat
     4081 gttgcttttt tataaatatt gaataagaat gtttgaaaat atcaaatcta atgactacat
     4141 ttgcagtcgc aaaaccagtt tagcgtttgc attggaagtg tgggtgagtg gctgaaacca
     4201 ccagtttgct aaactgacgt acgggaaact gtaccggggg ttcgaatccc cccgcttccg
     4261 caatatatac tgattatcaa gtgtttgcat caataaggga cta
//
LOCUS       DAOSMY010000112         4266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_20752_length_4266_cov_3.265495, whole genome shotgun sequence.
ACCESSION   DAOSMY010000112 DAOSMY010000000
VERSION     DAOSMY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20752_length_4266_cov_3.265495"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLD76_03550"
     CDS             complement(<1..189)
                     /locus_tag="PLD76_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU27109.1"
                     /translation="MIYLETSRQSEKRRGSIEVICGSMFSGKTEELIRRLKRAKIAQL
                     HVEIFKPAIDVRYDAEKIV"
     gene            254..982
                     /locus_tag="PLD76_03555"
     CDS             254..982
                     /locus_tag="PLD76_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27110.1"
                     /translation="MEKNILIDQMLKDIKELELIVNGVRTMNTIHPIMQQLVTRKFKN
                     LEENFSLVLQDSVPTDKGAPKFTDVNDEEDSITSTDDTTTTILLDEDIEEIEFVDTRK
                     KEQPVSQPTVKSSTSPTIPPSQNEPRTYTQQKSSIQPETTIHKETPKPASVILATPKT
                     SAVDARLVTDLRRAIGINDRFRFKRELFGGDEQKMMETIDILNQCSSLQEVEEYIDAH
                     FDWQKEDSTYVYFSEILHKRFAHI"
     gene            992..1705
                     /gene="rsmI"
                     /locus_tag="PLD76_03560"
     CDS             992..1705
                     /gene="rsmI"
                     /locus_tag="PLD76_03560"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU27111.1"
                     /translation="MKLFVVPTPVGNLDDMTFRAIEVLKKVDFILAEDTRTSAVLLKH
                     FDIKNTLIAHHKFNEHKGLQPIIERLLAGQTGAVISDAGTPGISDPGFLIVRACIEHN
                     IPVECLPGATAFVPALVNAGLPMDRFCFEGFLPQKKGRQTRLQQLKFETRTIILYESP
                     YRVVKTLTQLAEILGQNRKASVSREISKLYNETINGSLADIIAHFVQKQPKGEFVLVI
                     AGANVKHAELDDSSSEEDE"
     gene            1808..2659
                     /locus_tag="PLD76_03565"
     CDS             1808..2659
                     /locus_tag="PLD76_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27112.1"
                     /translation="MKKLLLASAILAILFSSCGYESKEFKALKAKNDSILAVQEQQIA
                     EMNEYMALVQEIDNGFSAIKQSQDMLSISSSYEGQPNESLRTRFTNDLNTINQILADN
                     KSKIADLEKKVESGKFQSSELRRTVNRLTETLNQKNQELEALSAVLAKKDITIDSLVV
                     ETQTLLRKTADLEMEKNVNLQEIAKQDADLNRVYYKLATRKEIKDSNIDVDKMKSTIR
                     NGMFTAADKRELDAVEINSKRARVLTKHPVSSYELRQKADKTYTLIIKNHTDFWSMSK
                     YLLVRID"
     gene            2738..3433
                     /locus_tag="PLD76_03570"
     CDS             2738..3433
                     /locus_tag="PLD76_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOU27113.1"
                     /translation="MKYKHLFFDLDNTLWDFDKNSQVSLHELFDAYQLIHYYPRFEDF
                     FEIFKKQNEVLWEMYGKGQIDKNFLHYERFRFPFENTPFEAQLRYKELADEYLLVVKT
                     KTHLKPFAKEILEYLKDKNYMMYIVSNGFTEVQYHKLENAGIGTYFQRIFLSESIKEH
                     KPSKAFFDYAITSSNARKTESLVIGDNWEADIVGAKNAGIDQVFYNYTNRIDLPFAPT
                     FQVSCLSDLKNLM"
     gene            3438..4220
                     /locus_tag="PLD76_03575"
     CDS             3438..4220
                     /locus_tag="PLD76_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU27114.1"
                     /translation="MHLIDTHSHIYLSDFDADREEVVLRAQSVGVKHVILPNIDASTI
                     QPLHVTAHTYAGLCHPLIGIHPTSVKADYKDEIAFFKAQFEQYPYIGIGEIGIDLYWD
                     KTYLHEQIEVFEYQVDMAIKHALPVIIHCRESFREIMQSLQHFDAKQLKGIFHSFTGT
                     PDEANEIFGAGNFKLGINGVVSYKNAIFAQQLQHIPLSAIVLETDAPYLTPVPHRGKR
                     NEPSYLPHVVKKLAEIYGVSEEEIAQTTSNNAYQVFPSLIDV"
BASE COUNT         1486 a          685 c          814 g         1281 t
ORIGIN      
        1 tacaattttc tccgcatcgt agcgtacatc aatggcaggt ttaaatatct ctacatgtaa
       61 ttgtgcaatt tttgcccgtt ttagacgacg gataagttct tcggtttttc cagagaacat
      121 agatccgcaa ataacttcga ttgaacctct tcgtttttct gattgacgtg atgtttctaa
      181 atatatcatt tgttgctttt gagtttaatt actacttttg caaaaataat taatttcttc
      241 agaaaaaagg ctcatggaaa aaaatatttt aattgaccaa atgctcaaag atatcaaaga
      301 gcttgaatta attgtgaatg gtgtgcgtac aatgaatacg attcatccaa taatgcaaca
      361 attagtaact agaaagttta aaaatttgga agaaaacttt tcgttggtat tgcaagatag
      421 tgtgcctacg gataagggtg ctccaaagtt tactgatgtt aacgatgaag aagatagtat
      481 taccagtaca gacgatacta ccacaactat tttgctggat gaggatattg aagaaattga
      541 gtttgtggat acacgcaaaa aagagcaacc agtttcccaa ccaacagtaa agtcaagcac
      601 atcacctact attccacctt cacaaaatga accgcgtacg tatactcaac aaaaatctag
      661 tattcaacca gaaacgacca tccataagga aaccccaaag cctgcttctg tcattttagc
      721 aacacccaaa acaagtgctg tggatgctcg acttgtaacc gatttgcgta gagcgattgg
      781 tattaatgat aggtttcgtt ttaaacgcga attatttggt ggagatgaac aaaaaatgat
      841 ggagacaata gatatattaa atcaatgttc gtcattgcaa gaggtagaag agtatattga
      901 tgcccatttt gattggcaaa aagaggatag tacatacgtc tatttttcag aaatattaca
      961 caaacgtttt gctcatatat aaaaaaattg tatgaaacta tttgtagtgc ccacgccagt
     1021 aggaaatttg gacgatatga catttcgggc tatagaggtg ctcaaaaaag tcgattttat
     1081 tttagccgaa gacacacgaa caagtgctgt tttactcaag cattttgata ttaaaaatac
     1141 ccttattgct catcataaat ttaacgagca caaaggatta caacccatta tagaacgctt
     1201 gttagcagga caaacagggg ctgttatttc tgatgctggg acacctggta tttctgatcc
     1261 tggttttttg atagtacgtg cctgtatcga acacaatatt ccagtagaat gtttgccagg
     1321 agcaacagct tttgttcctg cattagttaa tgctggtttg cccatggacc gtttttgttt
     1381 tgaaggcttt ttaccccaaa aaaaaggcag acaaacacgt ttgcaacaat taaaatttga
     1441 aacgcgtacc attattttgt acgaatctcc gtatcgggta gtgaaaacat taacgcagtt
     1501 ggcagaaata ttgggacaaa atcgtaaagc ctctgtatcg cgcgaaatat caaaactgta
     1561 caacgaaaca attaatggct ccttggcgga tatcatagct cattttgtgc aaaaacaacc
     1621 caaaggtgaa tttgtgctag taattgcagg tgcaaatgta aaacatgcgg aattggatga
     1681 ctcttcatca gaagaggatg aatgaaattt tatccaaatt tttatctaaa aaaataattg
     1741 atttgtaatt gattatgtat ctttgtaacc gaaaaacaca agtaaaactt ttttaaaaca
     1801 ttattttatg aaaaaattac tattagcatc tgcaattctt gctattctgt tttcatcatg
     1861 tgggtatgaa agcaaagaat tcaaggctct taaagcaaag aatgattcta ttttggcagt
     1921 acaagaacaa caaattgccg aaatgaatga atatatggct cttgttcaag aaattgataa
     1981 tggcttttcg gctatcaaac aatcgcaaga tatgttgagt attagttctt cttacgaagg
     2041 tcaacctaac gaatcattgc gtacacgttt tacaaatgat ttgaacacga ttaatcaaat
     2101 tttagccgat aacaagtcaa aaattgccga cttagagaaa aaagtagaaa gcggaaagtt
     2161 tcaatcgtca gaattgcgtc gtaccgtaaa tagactaacc gaaacattaa accaaaaaaa
     2221 tcaagaacta gaagcattaa gtgctgtgtt ggctaaaaaa gacataacga ttgatagttt
     2281 agtggtagaa acacaaacat tgttacgcaa aacagctgac ttggaaatgg aaaaaaatgt
     2341 aaatttgcaa gaaattgcca aacaagatgc tgatttaaat cgtgtgtact ataaactggc
     2401 tacacgcaaa gaaattaaag atagcaatat tgatgtagat aaaatgaaat caactatccg
     2461 caatggaatg tttaccgctg ccgataaacg tgaattggat gctgttgaaa ttaattcgaa
     2521 acgtgctaga gtattaacta agcacccagt tagttcatac gagttacgtc aaaaagctga
     2581 taaaacatat accttgatta ttaaaaacca tactgatttt tggagtatgt ctaaatattt
     2641 attggtacgt attgattagt aaaaagttaa ataaaggtgg taaaagtacg caagaattta
     2701 ttttctgcgt acttttgttt ttatatcgtg gtaagaaatg aaatataaac atctattttt
     2761 tgatttagat aataccttgt gggattttga taaaaattcg caagtaagct tgcacgagct
     2821 tttcgatgca taccagctaa tacattacta cccaaggttt gaggattttt ttgagatatt
     2881 taagaaacaa aacgaagtgt tatgggaaat gtatgggaaa gggcagatag ataaaaattt
     2941 tttgcactac gagaggtttc gttttccttt tgaaaataca cccttcgagg cacaattgcg
     3001 ttataaagag ttagccgatg agtatttgtt ggtagtgaaa acaaaaacgc atcttaaacc
     3061 ttttgccaaa gaaatattgg aatacctcaa ggataaaaat tacatgatgt acattgtatc
     3121 caacggattt acagaggtgc aataccataa actggaaaat gcaggtattg gtacctattt
     3181 tcaacgtata tttttatccg aatccattaa agaacataaa ccatcgaaag cattttttga
     3241 ttatgcaatt acatcatcaa atgctcgaaa gaccgaaagt ttggtgattg gtgataactg
     3301 ggaagcagat attgtaggag ctaaaaatgc agggatagac caggtttttt acaattatac
     3361 gaaccgtatt gatttgccat ttgcccctac atttcaggta agttgtttgt ctgacttaaa
     3421 aaatcttatg taatcctatg catctgattg atacacactc tcatatatat ctttccgatt
     3481 ttgatgctga tcgggaggag gttgttttgc gggcacaatc tgttggcgta aaacatgtta
     3541 ttttgccaaa tattgatgca tctaccatac agccattgca tgtcaccgcc catacatatg
     3601 cggggttgtg tcatccatta ataggtattc atccaaccag tgttaaggct gattacaaag
     3661 atgaaattgc gttttttaaa gcacaatttg agcagtatcc gtatatcggg ataggtgaaa
     3721 ttggcattga tttgtattgg gataaaacgt atctgcacga acaaatagaa gtttttgaat
     3781 atcaagtaga tatggctatc aaacatgcat tgcccgtgat catacattgc agggagtcgt
     3841 tcagagaaat tatgcaaagt ttgcagcatt ttgatgcaaa acaactaaaa ggaatttttc
     3901 atagttttac aggtactcct gatgaggcaa atgaaatttt tggagcaggt aattttaagt
     3961 tgggcatcaa tggtgtagta agttataaaa atgctatctt tgcacagcaa ttgcaacaca
     4021 taccattgtc ggctattgta ttagaaacag acgctcccta tctaacacca gtgccacata
     4081 ggggaaaaag aaacgagcca tcatatttgc cgcatgtagt aaaaaagcta gctgaaatat
     4141 atggagtaag tgaagaagaa attgcacaaa caacaagcaa taatgcttat caagtatttc
     4201 caagcttgat agacgtgtaa aaaaaaggag aggtgctcga gtggttgaag aggcacgcct
     4261 ggaaag
//
LOCUS       DAOSMY010000113         4213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21117_length_4213_cov_2.636123, whole genome shotgun sequence.
ACCESSION   DAOSMY010000113 DAOSMY010000000
VERSION     DAOSMY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21117_length_4213_cov_2.636123"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLD76_03580"
     CDS             complement(<1..231)
                     /locus_tag="PLD76_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOU27115.1"
                     /translation="MKKDSRILVFDNYDSFTYNLVHMIKKLGYNNVEVHRNDKIALAD
                     IAHFDKILLSPGPGVPSESGILLDLITTYAPTK"
     gene            complement(245..1642)
                     /locus_tag="PLD76_03585"
     CDS             complement(245..1642)
                     /locus_tag="PLD76_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOU27116.1"
                     /translation="MFKVFTKKMLGDIQTPVSIYLRVRDLYSQSALLESSDYHGGENS
                     FSFIAFNPLASFEVANNTMKASCIGNETIEKNIVDKFSVPTFFNEFVQSFSIAYQDEK
                     PVVNGFFGYTSFESVRYFDDIRVANKPLHEYDMPDMHYIFYRYIIAVNHFKNELLLIE
                     NVPDGEQSTLETIETLLYNLNLSTYSFKGIGAETSDITDAEYKAMVTEGKKACFRGDV
                     FQIVLSRRFSQAYEGDDFTLYRHLRSINPSPYLFYFDYGSFRIFGSSPEAHLVVNESK
                     AYINPIAGTFKRTGDDAKDLLLAEQLRMDPKENAEHVMLVDLARNDLSRNTHHVKVDI
                     FREVQYFSHVLHLVSQVSGEILPDTNVIQLFSDTFPAGTLSGAPKVRAIEIINQLEKH
                     NRGFYGGCIGYIGFDGGFNQATTIRSFLSKNNTLYYQAGAGVVSKSDEESELQEVNNK
                     LAALTAAIKKASNTK"
     gene            complement(1667..2848)
                     /gene="trpB"
                     /locus_tag="PLD76_03590"
     CDS             complement(1667..2848)
                     /gene="trpB"
                     /locus_tag="PLD76_03590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOU27117.1"
                     /translation="MKYAVDNNGYYGKFGGAYVPEILHKNVSELQERYLAIMQDSAFQ
                     KELNQLLHDYVGRPTPLYFATRLSQFVGAKVYLKREDLCHTGAHKLNNALGQILLAKR
                     MGKTRIVAETGAGQHGVATATVCALMNMECVVYMGETDVKRQYLNVQRMYMLGAKVVP
                     VTSGNKTLKDATNEAIRDWTCNPKDTFYIIGSVVGPHPYPDMVARFQAVISEEIMWQL
                     QQHENTSYPDYVIACVGGGSNAAGSFYHFLEHDSVKLIAAEAAGLGVDTNESAATIHL
                     GKEGILHGSKTLIMQTDDGQIVEPYSISAGLDYPGIGPMHAFLAESGRAQVYAITDDE
                     ALQAALQLTRLEGIIPAIESSHALAVLAKHSFKPNDVIVLTVSGRGDKDMETYCNRLT
                     F"
     gene            3245..>4213
                     /locus_tag="PLD76_03595"
     CDS             3245..>4213
                     /locus_tag="PLD76_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU27118.1"
                     /translation="MPQTSQRGQIMPASPIRKLVPFSDKAKERGVKVYHLNIGQPDLH
                     TPEKALDAVRNIDRKILEYSPSDGLKSLRVKMLEYYQRFNIQVNLSDIIVTTGGSEAV
                     NFAFMSCLDPGDEILVPEPAYANYTAFAISAGAVVKPIVSSIEDGFALPDIARFEELI
                     TPKTKGILICNPNNPTGYLYTQEEMNQIRDLVKKHDLFLFSDEVYREFCYTGAPYISA
                     FHLEGIEENVVLFDSFSKRYSECGIRVGALVCKNKAVKESVMKFCQARLSPPLLGQIV
                     AEASIDTPKEYLEEMYNEYIERRNFLIEGLNKIPGVYSPLPMGAFYT"
BASE COUNT         1288 a          842 c          726 g         1357 t
ORIGIN      
        1 tttggttggt gcgtacgttg taattaaatc tagcaaaatt ccagactcac taggtactcc
       61 aggaccaggt gatagcaaaa ttttgtcaaa atgggcaata tctgccaatg ctattttatc
      121 gtttctatga acctccacat tattataacc tagtttcttt atcatgtgaa ctaaattata
      181 ggtaaacgaa tcgtaattat caaatactaa aatgcgacta tcttttttca tctcagtgtt
      241 ctccttattt tgtgtttgat gctttcttaa tggctgccgt taatgctgcc aacttgttat
      301 tcacttcttg taattcactt tcttcgtcag attttgacac gacacccgca cccgcttgat
      361 agtacaaggt attgttttta ctcaaaaaag aacgaatggt agttgcttgg ttaaaaccac
      421 catcaaaacc gatatagcca atgcatccgc catagaagcc tctattgtgt ttttctaact
      481 gattgattat ttcgatggct cgcaccttgg gtgcacccga tagtgtgcca gctggaaagg
      541 tatctgagaa tagctgaata acatttgtat ctggcaaaat ttcgccactt acttgagata
      601 caagatgtaa tacatgcgaa aaatattgca cctctctaaa tatatctacc tttacgtggt
      661 gtgtgtttct actcaaatca ttacgtgcca aatctactaa catcacatgc tcggcattct
      721 ctttcggatc catacgcaat tgctctgcta ataacaaatc tttggcatca tcgccagttc
      781 gcttaaatgt acctgcaata ggattgatgt atgcttttga ttcatttaca accaagtgag
      841 cttcgggcga agacccaaaa atacgaaaag agccataatc aaaataaaac aaatacggag
      901 atggattgat agaacgcaaa tgcctatata gggtaaaatc atcgccttcg taggcttgcg
      961 aaaaacgcct tgataataca atttggaaaa catcgcctct aaaacaggct tttttacctt
     1021 ctgtaaccat tgccttatat tctgcatccg taatgtcaga cgtttctgcc ccaatgcctt
     1081 taaaagagta tgtagataag ttgagattat acaacaacgt ttcaatggtt tcaagcgtag
     1141 attgctctcc atcaggcaca ttttctatca agagcaactc atttttaaaa tgattgacag
     1201 caataatata ccgataaaag atatagtgca tatctggcat atcgtattcg tgtagtggtt
     1261 tgttagcaac acgaatatca tcaaaataac gaacagattc aaagcttgta taaccaaaaa
     1321 atccatttac gacaggtttt tcatcttgat atgcaatgga aaatgactgc acaaattcat
     1381 taaaaaatgt gggtacagag aatttatcca ctatattttt ttcaatcgtt tcgttgccaa
     1441 tacagcttgc tttcattgta ttgttggcta cttcaaaaga tgccaaggga ttgaatgcta
     1501 taaacgaaaa gctattttcg ccaccatggt agtcggaact ctctaacaat gctgattgtg
     1561 aatacaaatc tcgcaccctt agatatatac ttacaggagt ttgtatatct cctagcattt
     1621 tttttgtgaa tactttaaac attgtatttt aaaatttaat aagtaattaa aacgttaatc
     1681 gattgcaata ggtttccata tctttatccc ctctacccga tacggtaagc actattacat
     1741 catttggttt aaaggaatgt tttgccaata ctgccaatgc atgagaactt tcgatggcag
     1801 gtataattcc ctccaaacgt gtaagttgaa gtgctgcttg caatgcctca tcatcggtta
     1861 tggcatacac ttgtgctctc cccgactcgg ctaaaaacgc atgcatcgga ccaatacctg
     1921 ggtaatctaa tccagccgaa atagaataag gctcaacaat ttgtccatca tccgtttgca
     1981 taatcaaggt tttactacca tgcaaaatac cctctttacc aagatggatt gtagctgctg
     2041 attcattggt atccactccc aaaccagctg cttctgctgc aattaatttc accgaatcat
     2101 gctccaaaaa atgataaaaa gaacctgcgg cattgctacc tccacccaca catgctatta
     2161 cataatcggg gtacgatgta ttttcgtgtt gttgtaactg ccacattatt tcttcgctaa
     2221 taaccgcctg aaatcgagct accatatccg ggtaaggatg aggtcctacc acagaaccaa
     2281 taatataaaa cgtatctttc ggattgcatg tccaatcgcg aatagcctcg ttagtagcat
     2341 cttttaaggt tttattacct gatgttacag gcacaacttt tgcacctaac atatacattc
     2401 gttgcacgtt aaggtactga cgttttacat ccgtttcgcc catatacact acgcactcca
     2461 tattcatcaa cgcacatacc gttgctgttg ctacaccatg ttgtcctgca cctgtttcgg
     2521 ctacaatacg ggttttcccc atacgttttg ccaataaaat ttgtccaagt gcattgttta
     2581 atttatgtgc acctgtatga cataaatctt ccctttttaa atacactttt gccccaacaa
     2641 attgagataa tctcgtagca aaataaagag gagtaggtct gcctacatag tcatgcaaca
     2701 gctggttgag ttccttttga aaagcactat cttgcataat agcaaggtaa cgctcttgaa
     2761 gttcagaaac atttttgtgt aatatctctg gcacataagc accaccaaat ttaccataat
     2821 atccattgtt atctactgcg tatttcatct gttcgttata tattttaata gtttacattc
     2881 atttttatca acaaaaaagg tctgtcgtga gttcgacaga cctttcggta tatgcttatt
     2941 aatatgttgg tagtacacac aataggcttg ccactcacga atgattgtat cgtaagcgcc
     3001 accaccaatt tgtatttact actagtttca tagtcattat tgaattacga tgcaaatttg
     3061 ttgctttttt tttagataaa caagtttttt gatgtttttt tttacaatag tgcttatttt
     3121 tatcttcaat ataaaaggga cataacctca atttttatct tctacacaaa tattaaaacc
     3181 tacaactttc agaaataata attttatact aactttgcag actttatgat aatataaaaa
     3241 cgctatgcct caaacgtcac aacgtgggca aataatgcct gcttctccta ttcgcaaact
     3301 agtccctttt tctgataaag caaaagaaag aggagttaaa gtgtaccatt taaatattgg
     3361 tcagcccgat ttgcataccc ccgaaaaggc tttagatgcg gtacgcaaca ttgatagaaa
     3421 aattcttgaa tatagcccca gtgatggtct aaaatcatta cgggtaaaaa tgttggaata
     3481 ctaccaacgg tttaacattc aagtaaactt gtcggatatt attgtaacga ctggaggctc
     3541 tgaggcagta aatttcgcat ttatgtcctg ccttgaccct ggggatgaga tattagttcc
     3601 agagcctgca tacgctaact atactgcgtt tgctattagt gcaggggctg tggttaaacc
     3661 aattgtatct tcgattgaag atgggtttgc attgcctgat attgcccgtt ttgaagagct
     3721 aattacgccc aaaacaaaag gcattttaat ttgcaatccc aacaacccta caggctattt
     3781 atacacgcaa gaggagatga atcaaattag ggatttggtt aagaaacatg atttgttctt
     3841 attttctgac gaagtgtacc gcgagttttg ttacacagga gcaccctata tttctgcttt
     3901 tcatttagaa gggattgaag aaaatgtggt attattcgat tctttctcaa aacgctatag
     3961 cgaatgtggt attcgtgtag gtgcattagt ttgtaaaaat aaagcggtga aagaaagtgt
     4021 gatgaaattt tgtcaagcac gtttaagtcc acctttatta ggacagatag tagccgaagc
     4081 ttctattgat acgccaaaag aatacttaga ggaaatgtat aacgagtaca tcgaacgcag
     4141 aaatttcttg attgaaggat taaataaaat tccaggcgta tattctccgc ttccaatggg
     4201 tgctttctat aca
//
LOCUS       DAOSMY010000114         4167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21427_length_4167_cov_3.127918, whole genome shotgun sequence.
ACCESSION   DAOSMY010000114 DAOSMY010000000
VERSION     DAOSMY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_4167_cov_3.127918"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /gene="carB"
                     /locus_tag="PLD76_03600"
     CDS             complement(<1..1627)
                     /gene="carB"
                     /locus_tag="PLD76_03600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOU27119.1"
                     /translation="MNENLIPGIKKVIVLGSGALKIGEAGEFDYSGSQALKALKEEGI
                     YTVLVNPNIATIQTSEGVADQIYFLPITPFFVEKVIQKEKPDGILLAFGGQTALNCGV
                     QLHRLGILEKYNLRVLGTPVQAIMDTEDRDLFVKKLDQIDVKTIKSFAVDNMEDARKA
                     AKDLGYPIIIRAAYALGGLGSGFCDNEEELDAMAEKSLSFSPQILVEKSLKGWKEVEY
                     EVVRDRFDNCITVCNMENFDPLGIHTGESIVIAPSQTLTNAEYHKLRELAIRIIRHIG
                     IVGECNVQYALDPESEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFDV
                     KNSVTKTTPAFFEPALDYVVCKIPRWDLGKFQGVSRELGSSMKSVGEIMAIGRTFEEA
                     VQKGLRMIGQGMHGFVANKELVIADIDKSLNEPTDNRIFVISKAFRKGYTIDQIHDLT
                     KIDKWFLQRLYKIVETANELEAFNSVEDVDDELFRLAKQQGFSDFQIARLTSDSAESE
                     ESLIKVRNNRKARGITPVVKRIDTLAAEYPAQTN"
     gene            complement(1627..2697)
                     /gene="carA"
                     /locus_tag="PLD76_03605"
     CDS             complement(1627..2697)
                     /gene="carA"
                     /locus_tag="PLD76_03605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOU27120.1"
                     /translation="MQKSKAAKLVLENGVVFHGYSFGYEQSKAGEVVFNTAMMGYPES
                     LTDPSYSGLILAFTFPLIGNYGVPSSNVTHQIADFFESEKIHASGVVVSTYSFEYSHW
                     NAKQTLSDWLIENKVPGIYGVDTRELTKVLREKGAMKGKIVIEDADVDFIDSNAINQV
                     NIVSCKEVMHYGNGKKKVVLVDCGVKHNIIRCLLKRDVTLIRVPWNYDFNTLEFDGLF
                     LSNGPGNPSTCAETVEHIRKAMKRNVPIFGICMGNHLLAKAGGAETYKLKYGHRSHNQ
                     PVRMVGTNRCFVTSQNHGYAVDNTTLSEEWEPLFQNMNDDTNEGLKHKSKPFFSVQFH
                     PEAASGPTDTEFLFDEFIKMID"
     gene            2908..3627
                     /locus_tag="PLD76_03610"
     CDS             2908..3627
                     /locus_tag="PLD76_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU27121.1"
                     /translation="MARNSFFEFKQFRINQENCAMKVGTDGVLLGAWTPIPLSTTRIL
                     DIGTGSGLLALMLAQRTHAHIDAVEIDTLAAQQAKENFISSPWSNRLQIYETDFQSFA
                     KNELHKYDVIVSNPPYFHNSLHTPNRQRTIARHSDSLTLDELVDGVEKLLQPWGYFCV
                     ILPTHESEIVAKKALLANLYCTQKTFVYPTPTSPAKRIMQLFSKKETTPLTQHLVIEQ
                     EQRHQYTKEYSELTKDYYLNF"
     gene            3723..4097
                     /locus_tag="PLD76_03615"
     CDS             3723..4097
                     /locus_tag="PLD76_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU27122.1"
                     /translation="MTTRLLILSILIFTITSCTSQTSSFKSVDAKEFEQILRTDTLQI
                     VDVRTPEEFNQGHIQGAICMDVRNPDFDSKIIQLDKHIPVAIYCRAGRRSKIAAEKMS
                     AIGLQGYELDNGIVSWENELTR"
BASE COUNT         1316 a          840 c          705 g         1306 t
ORIGIN      
        1 aatttgtttg agctggatac tcagcggcta aggtatcaat acgttttact acaggagtaa
       61 tacctctagc tttgcggttg tttcttactt ttatcaaact ttcttcactc tcagcactat
      121 cgcttgtaag acgtgctatt tggaagtcag aaaagccttg ttgtttagcc aaacggaata
      181 attcatcatc aacatcttct accgaattga atgcttccaa ttcgttggct gtttcaacaa
      241 ttttatataa tcgttgtaaa aaccatttat ctattttggt taaatcatga atttggtcga
      301 tggtataccc tttacgaaat gcttttgaga taacaaaaat acggttatct gttggttcgt
      361 ttagcgattt gtcaatatca gcaattacca gttctttatt agcaacaaaa ccgtgcattc
      421 cttgaccaat catacgcaag cctttttgca cagcttcttc aaacgtacgt ccgatagcca
      481 tgatttcgcc tactgatttc atgcttgagc ctaactcgcg cgaaacgcct tgaaacttgc
      541 ccaaatccca acgaggaatt ttacacacca catagtcgag tgcaggttca aaaaacgcag
      601 gagtagtttt agttactgaa tttttgacat cgaacaaccc ataccccaat cccaattttg
      661 cagctacaaa agccaatgga tagcctgtag ctttagaagc caaagccgaa gagcgactta
      721 aacgggcatt tacctcaatt acacgataat cttccgattc tgggtcgaga gcatattgca
      781 cattacactc acccactata ccgatatggc gaatgattcg gattgccaat tcacgcaatt
      841 tgtggtattc ggcattcgta agcgtttgcg aaggagcaat aacgatactt tcgcccgtat
      901 gaataccgag cgggtcgaaa ttctccatat tacataccgt aatacagttg tcaaaacggt
      961 cgcgaactac ctcgtactca acctctttcc acccttttaa tgatttttcg actaatattt
     1021 ggggagagaa agacaatgat ttttcagcca ttgcatctaa ttcctcttca ttatcacaaa
     1081 atccactacc taaaccacct aaggcataag ccgcacgtat aataattgga taccccaaat
     1141 ctttagcagc tttgcgagca tcttccatat tatctacagc aaaacttttg attgttttta
     1201 catcaatttg gtcgagtttt ttcacgaaca aatcgcggtc ttctgtatcc ataattgcct
     1261 gtacgggagt tcccaataca cgcaaattgt atttttctaa aattcctaat cggtgcaatt
     1321 gtacaccaca gttaagagct gtttgaccac caaaagccaa caaaatacca tcaggttttt
     1381 ctttttggat gactttttcg acaaaaaatg gcgtaatggg taagaaataa atctggtcgg
     1441 ctaccccttc ggatgtttga atggtagcaa tatttggatt gaccaaaacg gtgtaaattc
     1501 cttcttcttt tagtgctttc agagcttgag agcctgaata atcaaattcg ccagcttccc
     1561 caattttgag tgctcctgag cctaaaacaa taactttttt tattcctggt attaaattct
     1621 cgttcattag tcaatcattt taataaattc gtcaaataaa aattctgtat cagtaggtcc
     1681 acttgctgct tcggggtgaa attggactga aaagaaaggt tttgatttat gttttagccc
     1741 ttcgttggta tcatcattca tattttggaa aagaggttcc cattcttcac tcaaggtagt
     1801 gttatctaca gcatatccat gattttgtga agttacaaaa caacgatttg ttcccaccat
     1861 acgcacgggt tggttgtggc tacgatgacc atattttagt ttatatgttt cagcaccacc
     1921 cgcttttgcc aacaaatgat tgcccatgca aataccaaat atgggtacat tgcgtttcat
     1981 tgctttgcgt atatgttcta cagtttcagc acatgtagat gggttgccag gaccattaga
     2041 caaaaataat ccatcaaatt cgagggtatt aaaatcataa ttccaaggca cacgaatgag
     2101 cgttacatct ctttttaata agcaacgaat gatattgtgt tttacaccac aatcgaccaa
     2161 tactactttt ttctttccat ttccgtagtg cattacctct ttgcacgaca cgatgtttac
     2221 ctgattaatt gcatttgaat cgataaaatc aacatcagca tcttcgataa cgatttttcc
     2281 tttcattgca cctttttcgc gcaatacttt tgtcaattca cgcgtatcaa cgccataaat
     2341 acctggcact ttgttttcaa tcaaccaatc gcttagggtt tgttttgcat tccagtggct
     2401 gtattcaaac gaatatgttg acactactac gcccgaagca tgaatttttt cggactcaaa
     2461 aaaatcggca atttgatggg ttacattgga agaaggaaca ccataattgc cgattaaagg
     2521 gaatgtaaat gctaagatta gacccgaata ggaagggtct gttaaacttt cgggatagcc
     2581 catcatggca gtgttaaaaa ccacctctcc tgctttcgac tgttcataac cgaaggagta
     2641 cccatgaaaa acgactccat tctctaacac caacttggca gctttgcttt tttgcatgtt
     2701 ttactaatat aaatatgtta caatgagtag ttctcaaaaa ttgcacaaag atacaatatt
     2761 ttagcattcc aaacaaacag gattttgcta tttttcaaca tttttacacg aataataaat
     2821 tgatttgttc gatagccata ttgatgggag ttagacactg tttagtatat gaaaagttta
     2881 ctgtatattt gcatttttaa catatttatg gcaagaaatt cgttttttga gtttaaacaa
     2941 tttagaatta accaagaaaa ttgtgctatg aaagttggca cagatggtgt gctgcttggg
     3001 gcatggactc ccataccact tagcactacc cgtatattgg acattggaac aggaagtgga
     3061 ttacttgctc tgatgctcgc tcaacgcaca catgcccata ttgatgccgt agaaatagac
     3121 accttggcag ctcaacaagc caaagaaaat ttcatatctt ctccttggag caatcgatta
     3181 cagatttatg aaaccgattt tcaatcattt gccaaaaacg aattacataa atatgatgtt
     3241 attgtatcaa atccccctta ttttcacaat tcattgcata ctccaaacag acaacgtaca
     3301 atagctcgac atagtgattc gcttacgcta gacgaattag ttgatggcgt tgaaaaatta
     3361 ctacaacctt ggggatattt ttgtgtcata ttacctacac atgaaagtga aatagtagct
     3421 aaaaaggctt tgctggcaaa tctttattgc acacaaaaaa cgtttgttta cccaacacct
     3481 acgagcccag ccaaacgcat catgcaacta ttttcgaaaa aagaaacaac cccgcttaca
     3541 cagcatttgg taattgaaca agaacaaaga catcagtata ccaaagaata tagtgaactt
     3601 acaaaagatt attatctaaa tttttgaaga ggaaaaacac taattcaaaa aaaaatatca
     3661 ttccaagttt attaattatg tgtacatttg tggtaattat gcatacagta acataaaaaa
     3721 atatgacaac ccgtttactt attttaagta tacttatttt caccatcaca agttgcacca
     3781 gtcaaacaag ttcttttaaa tcagttgatg caaaagagtt tgagcaaata ttacgtacag
     3841 atactcttca aattgtggat gtacgcacac ctgaagaatt taatcaagga catattcaag
     3901 gggctatttg catggatgtt agaaatccag attttgactc aaaaataata caattggata
     3961 aacatatccc tgtggctatc tattgcagag ctgggagaag aagtaaaatt gctgctgaaa
     4021 aaatgagtgc aattggacta caaggatatg agctagataa cggcattgtg tcttgggaaa
     4081 atgaattaac tcgttaacta ttttgtaaaa ggacaataaa acgaatcatc cctatactga
     4141 taatacctct atctggtata tatccac
//
LOCUS       DAOSMY010000115         4123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21736_length_4123_cov_3.644051, whole genome shotgun sequence.
ACCESSION   DAOSMY010000115 DAOSMY010000000
VERSION     DAOSMY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_4123_cov_3.644051"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="PLD76_03620"
     CDS             <1..1579
                     /locus_tag="PLD76_03620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU27123.1"
                     /translation="IESMLKNNILEEKYYTLVSNSFNANDIDTKYAFDARTITTGLEF
                     VAQPYFSLSDSLFQVTNKEIKDRYKRDQIRYTQQDETRDIAIVTFDIKPSEQDFQEVK
                     LWIEKLKNEFSTSTDFVSIANQNSDVPTSDIAVSRNNIDPDLKEFAFTASVGDVFGPI
                     YVNGTYKMARLSEKGILSSDSAKVRHILIQEPTVEATQKLVDSLFNVLKGGADFATVA
                     QQYSKAGTKAQGGELGWIKEGEIDKEFSTAAINAKVNEFFTLKLGSGIHIVQVTEKTK
                     PIEKVKLASIIRKVEPSSRTFGVIFNQASQYIAQNRTIEKFEATANSENGLSVRTYTV
                     SANDNKIANIKESRQIIRWAYEKNEGDVTDKVFESGEQFVAVALKSVNPKGTKSLSKV
                     TEEIKAELIKEKKADKLAADLTTKLNQAKNLNALGLPLQVVESVSFETPFVPSMGREP
                     KVLGNIVSLIKTQQPKVIKGNTGVFVVQAVNVGVQPAFNKKEEMNLVQQKMFSQNVLV
                     ESLKRTADIEDNRIRFY"
     gene            1732..3657
                     /locus_tag="PLD76_03625"
     CDS             1732..3657
                     /locus_tag="PLD76_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOU27124.1"
                     /translation="MRKIIGLIVSLLFISQFTFSQILTPVKWTFEISDIDDSSKEIVA
                     TASIDEHWKMYGTNLPEGGPISTVFIIEAITGASIEGDFLSSSQPKLYYDAMFQMELS
                     SYEKKAVFTQRLKIIDKANFSVKGYVRYMACDATSCLPPTEASFSFGSEPSQTITMQH
                     AIDTPFWEPVIDDLQLVGNGAVVPQQSYWIIFVFGFLGGLLALLTPCVWPMIPMTVSF
                     FLKSSKNKNKAIKNALIYGFSIIIIYLGLGLLVTGIFGASSLNSLATSAIFNILFFIL
                     LIVFALSFFGLFEITLPSSWVNAMDKKVDTTTELVSIFFMAFTLVLVSFSCTGPIIGT
                     LLVEAASTGSVVGPAVGMFGFALALAIPFAFFSLFPSYLQSMPRSGGWLNMVKVTLGF
                     LELALALKFFSVADLAYGWGLLSRDLFIGIWIIIFIILGLYYLRVIRFPMDKDDKKIT
                     LPRIIFALVSFLFVVYLLPGMWGKQRASISAFTPPMSTQIWNKNKDHVQAMFEDYDIA
                     MKYAQTVDKPVLIDFSGYGCVNCREMEATVFEDEAIKKMLKEDFIFVVLYVDDKKNLS
                     EPMLVSENNATRTLRTQGDKWSYLQRLKFGANAQPYYVVLNAAGKPVAPSYAFNKDVK
                     AFETYLKQAIKTYKNEK"
     gene            3647..>4123
                     /locus_tag="PLD76_03630"
     CDS             3647..>4123
                     /locus_tag="PLD76_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU27125.1"
                     /translation="MKSNHAEAIIEGLNSSQKEAVMYNNGPSLIIAGAGSGKTRVLTC
                     KIAYLLELGLSPERIVALTFTNKAAREMKERIASMVGQKIAKRLVMGTFHAVFSRILR
                     TESEHLTIKKDFVIYDTTDAKNTIKTIVKEMQLDEKMYKPALVLSRISYAKNNLLSP"
BASE COUNT         1382 a          596 c          785 g         1360 t
ORIGIN      
        1 tattgaaagt atgcttaaaa ataatatact tgaagagaaa tattatactt tagtgtcaaa
       61 ttcctttaat gcgaatgata tagatacaaa atatgctttt gatgcacgta ctataactac
      121 tggattggag tttgttgctc aaccatattt ttcgttgtct gactcattgt tccaagtaac
      181 aaataaagaa attaaagatc gttataaacg agatcaaatt aggtataccc aacaagatga
      241 aacgagagat atagctatag taacatttga tatcaaacct tctgaacaag attttcaaga
      301 agtaaaattg tggatagaaa aattgaaaaa tgagttttct acttctactg actttgtttc
      361 aatagcgaat cagaattcag atgttccaac gagtgatatt gctgtaagca gaaataacat
      421 tgatcctgat ttaaaagagt ttgcatttac agcttcggtg ggtgatgtat ttggtccaat
      481 atatgtgaat ggtacctaca aaatggctcg tttgtcggaa aaaggtattc tatcgtcaga
      541 ctctgctaag gttcgtcata ttttaataca agagccaacc gttgaagcta cccaaaaact
      601 cgttgatagc ctgtttaatg tattaaaggg tggagctgat tttgcaactg ttgcacaaca
      661 atattccaag gcaggtacga aagctcaagg gggagaactt ggttggatta aggaaggcga
      721 aatagataaa gaatttagta ctgctgctat taatgcaaaa gtaaatgaat tttttacact
      781 caaattagga agtggtatcc atattgtgca agtaacagaa aaaactaaac caatagagaa
      841 agtcaaactg gcttctatta ttcgtaaagt ggaaccaagc tctcgaacat ttggtgttat
      901 ttttaaccaa gctagtcaat atattgctca aaatagaacg atagaaaaat ttgaagctac
      961 ggctaactct gaaaatggtt tatctgttcg tacatataca gtgtctgcaa atgacaacaa
     1021 aatagcaaat ataaaggaat ctcgtcaaat aatacgatgg gcgtacgaaa aaaatgaagg
     1081 agatgttact gacaaagtgt ttgaaagtgg ggaacaattt gttgcagttg cattgaaaag
     1141 tgtaaaccct aaagggacaa aatcattatc aaaagtaacg gaagaaatta aagctgagtt
     1201 gataaaagag aagaaagcag ataaattagc tgctgattta acaaccaagt taaaccaagc
     1261 aaaaaatctt aatgctttag gtttaccatt gcaagtagta gagagtgttt cctttgaaac
     1321 gccattcgtt ccgtctatgg gcagagagcc taaagtgcta ggaaatatcg tttctcttat
     1381 caaaacacaa cagccaaaag taattaaagg aaatactggt gtatttgttg tgcaagcagt
     1441 gaatgtaggt gttcaaccag ctttcaataa aaaagaagaa atgaatttgg ttcaacaaaa
     1501 aatgtttagc caaaacgtat tggttgaatc attgaaaaga acagcagata ttgaagataa
     1561 ccgtattcgt ttctactaat ctattctatt atcgaaggta taaaggcaaa catactttct
     1621 taataaaagt aaaccgaggt agatttatct cggttttttt tggtaacaat ttttgaaaga
     1681 tgtacttttg tatattaagg tagtatcact aatgttataa aatagtattg tatgagaaaa
     1741 attattggtt taattgtatc cctattattt atttcacaat ttactttttc acaaatattg
     1801 actcctgtaa agtggacttt tgaaatatca gatatagatg actcttcaaa agaaatagtt
     1861 gccacggcat ccatagatga gcattggaaa atgtatggaa caaatttacc tgaaggaggt
     1921 ccaatttcta cagtttttat aatcgaagct ataacaggag ctagtataga aggagatttt
     1981 ttatcttcta gtcaaccaaa attgtattat gatgctatgt ttcaaatgga gttgtcttcg
     2041 tacgaaaaaa aggcagtttt tactcaacgt ctcaaaatta tagataaagc caatttttct
     2101 gtcaaaggat atgtacgata tatggcatgt gatgcaactt cttgtttgcc acctaccgaa
     2161 gcatcgtttt cgtttggctc tgaaccatca caaacgataa ccatgcaaca tgcaatagac
     2221 actccttttt gggaacccgt aattgatgac ttacaattag taggaaatgg agctgttgta
     2281 ccacaacaat cgtattggat aatttttgtt tttggatttc ttggtggatt attagcctta
     2341 cttaccccat gtgtatggcc catgatacct atgacagtta gtttcttttt aaaaagctct
     2401 aaaaataaaa acaaagcaat taaaaatgct ttgatatatg gtttttctat tatcattatt
     2461 tatttaggtt taggtttatt ggtaacagga atttttggag caagttcgct gaatagttta
     2521 gctactagtg ctatctttaa tattctattt tttattcttc taattgtatt cgcattgtca
     2581 ttttttggct tatttgaaat aacattacct tcatcatggg tcaatgcaat ggataaaaaa
     2641 gtagatacta ctactgaatt ggttagtatt ttctttatgg catttacatt agtacttgtt
     2701 tctttttctt gtacaggtcc tattattggt actcttttgg tagaagcggc tagtacggga
     2761 tcggtcgttg ggcccgcagt aggtatgttt ggttttgctt tggcgttggc aattcctttt
     2821 gctttcttct ccttgtttcc atcgtatctg caatctatgc cacgttctgg gggatggtta
     2881 aacatggtaa aggtaacctt agggttttta gaattagcat tggcattaaa attcttttca
     2941 gtagctgatt tggcttacgg atggggtttg cttagtaggg atttgtttat aggcatttgg
     3001 attattattt ttattatttt aggtttgtat tatttgcgtg ttatacgttt tccaatggat
     3061 aaagatgata aaaaaataac tcttccacgg attatctttg ccttggtatc tttcttattt
     3121 gttgtctatt tactgccagg gatgtgggga aaacaacgag cctctataag tgcatttaca
     3181 cctcctatgt caacacaaat atggaataaa aataaggatc atgtgcaagc tatgtttgaa
     3241 gattatgata tagctatgaa atatgctcaa accgttgata aacctgtttt aattgatttt
     3301 tcaggttatg gttgcgtaaa ttgtcgcgaa atggaagcaa ctgtatttga agatgaagca
     3361 attaaaaaga tgctgaaaga agattttatt tttgtagttt tgtatgttga tgataaaaaa
     3421 aatcttagcg agccaatgct tgtgtcagaa aataatgcta cgcgtacgtt gcgtacacaa
     3481 ggagataaat ggagctattt acaacgttta aaatttggag ctaatgccca gccatattat
     3541 gtggttttaa atgcagcagg caagccagta gctccttctt atgcttttaa caaggatgta
     3601 aaagcttttg aaacatattt aaagcaagcg attaagacct ataaaaatga aaagtaacca
     3661 tgcagaggct attattgaag gattaaattc ctctcaaaaa gaagctgtta tgtataataa
     3721 tggtccttcg ttgattattg ctggagcagg gtctggaaaa acacgtgtgt taacgtgtaa
     3781 aattgcttat ttattagaat taggtttgtc tcccgaacga atagtagctc ttacttttac
     3841 gaataaagct gccagagaaa tgaaggaacg cattgcctct atggtaggtc agaaaatagc
     3901 taaacgtttg gtaatgggga cttttcatgc tgttttttca cgtatattac gcacagaatc
     3961 ggaacattta actataaaaa aagattttgt aatatacgat acaactgatg ctaaaaatac
     4021 tatcaaaacc atagtaaaag aaatgcaact tgatgaaaag atgtataagc cagcattggt
     4081 gttgtcaaga atttcttatg caaaaaataa cttgctatca cca
//
LOCUS       DAOSMY010000116         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21742_length_4122_cov_3.409393, whole genome shotgun sequence.
ACCESSION   DAOSMY010000116 DAOSMY010000000
VERSION     DAOSMY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21742_length_4122_cov_3.409393"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLD76_03635"
     CDS             complement(<1..204)
                     /locus_tag="PLD76_03635"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOU27126.1"
                     /translation="MKIYTKTGDKGSTGLLGGTRVEKNDPRLEIYGTFDELTSFLGIL
                     KNQDIEDIHKKEIEYIQQNMIQVN"
     gene            complement(227..1012)
                     /locus_tag="PLD76_03640"
     CDS             complement(227..1012)
                     /locus_tag="PLD76_03640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU27127.1"
                     /translation="MWQQKIWHIFQFFRYKIYAKHRKGHGVHSPFVFAFITDILEEKQ
                     PYYCFEEIEKTRQLLCQNNQKIFVDDFGTGRSNHRKISSIARKSLKSKKFAQLIFRII
                     AFYKPTTIIELGTSLGITSSYIASANKQAKVFSFEGSTEIAKIAKNNMDTLHLSNISI
                     ITGDITTQLPLFLANSAQIDMIFFDANHKKEPTLSYFNMCMDKIGKKALFIFDDIYWS
                     KEMKEAWEEICADTRIQVSIDLFSIGIVFIDPELKKKHYTIYY"
     gene            complement(1048..2265)
                     /gene="hflX"
                     /locus_tag="PLD76_03645"
     CDS             complement(1048..2265)
                     /gene="hflX"
                     /locus_tag="PLD76_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU27128.1"
                     /translation="MISHSEIHIERVVLVGLITQQQNETKSKEYLKELAFLAETAGAI
                     VIKEFYQRVELPNTKTYVGKGKLLEIKNFIQEQEINLVIFDDELSPSQLRNIENTLEA
                     KALDRTSLILDIFAKRAQTAHAKTQVELAQYEYFLPRLTRLWTHLERQKGGIGLRGPG
                     ETQIETDRRIILDKIARLKAELIHIDKQKTIQRKNRGKMVRVALVGYTNVGKSTLMNL
                     LSKSNVFAENKLFATLDTTVRKITINNLAFLLSDTVGFIRKLPHGLVESFKSTLDEVR
                     EADILVHIVDISHPAFEEQIEVVEKTLHEIDNKEKLTILVFNKIDAFTYEKKDEDDLT
                     PLRKENISLQELQETWMSKLKQNCIFISAKEKTNIETLKDLLYERAKEIHTTRFPYND
                     FLYQQYDDDTSEE"
     gene            complement(2323..3009)
                     /locus_tag="PLD76_03650"
     CDS             complement(2323..3009)
                     /locus_tag="PLD76_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU27129.1"
                     /translation="MEIKKTPKANLENKKTMAILIGLVLALGIMFIAFEWSKNEIKVY
                     EDAIQGEFVEDEEMIEVTFRDETPPPPAPPIQETVLSDIIDIRDDKEDIQTKNFNSED
                     DKNKKVVIQAPIAAPVEDPEESRIHVVVERMPEFPGGEAAMNQFINRTIRYPVIAQEN
                     GIQGRVVVQFVVNTDGKIVDVEVVRGVEESLDKEAVRVVKAMPPWNPGRQGGKNVRVK
                     YTLPIRFRIG"
     gene            3211..>4122
                     /gene="porQ"
                     /locus_tag="PLD76_03655"
     CDS             3211..>4122
                     /gene="porQ"
                     /locus_tag="PLD76_03655"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOU27130.1"
                     /translation="MSVVLLFSTYIQAQSLFTMSFLEQPVSAHHAALGGNNTSYLGHD
                     ISFLVSNPALLSIQSSNELSVNVASYFAGSGYGSGIYSRLLHERGMLGAGFQYMGYGE
                     FTGFDEYGVETGTFSARDVAFFVSYSQQLNNYFSIGATVKPVLGVYERYVSYALAGDM
                     GIVFMDKENGLQVGFSAKNIGVRFAVYVSDESASMLPLNLSLGASKKLLHAPFVFHLT
                     MQQLQRWTYDVSTNVDSTSISFVGELARHFIVGVDVLPPSDKFWLALSYNFLRGQELP
                     VPEIFSLSGFSGGIGFFIKDVELGIGFG"
BASE COUNT         1054 a          652 c          799 g         1617 t
ORIGIN      
        1 attaacttga atcatatttt gttgaatata ttcaatttct tttttgtgta tatcttctat
       61 atcttggttt tttaagatcc ctagaaaact tgtcaattca tcaaatgttc cgtaaatttc
      121 taaacgtggg tcgttctttt ctactctagt tcctcctagt agcccagtgc tacctttgtc
      181 tccagtcttt gtgtatattt tcatgagttg aaacgtgttt tgtgatttaa tagtaaattg
      241 tatagtgttt tttctttagt tcggggtcaa taaaaacgat accgatacta aataaatcga
      301 tggatacttg aatacgggtg tctgcacata tttcttccca tgcttctttc atttctttcg
      361 accagtaaat atcatcgaat ataaatagtg ctttttttcc aattttatcc atacacatat
      421 tgaaataaga tagtgtaggt tcttttttgt ggttagcgtc aaaaaatatc atatcaattt
      481 gagctgaatt ggctaagaag agtggaagtt gagtagtaat atctcctgta attatgctaa
      541 tattggaaag gtgaagtgta tccatgttgt ttttggctat tttagcaatt tcggtagatc
      601 cttcaaatga aaaaacctta gcttgtttat ttgcagaagc tatgtaagag cttgtaatgc
      661 ctaggcttgt tcctaattca ataatagtag taggtttgta aaatgcaatg attcgaaaaa
      721 ttaactgagc aaattttttg cttttgaggc ttttgcgagc gatagaactg atttttctat
      781 gatttgagcg tccggttcca aaatcatcca caaatatttt ttgattattt tggcacaaaa
      841 gttggcgtgt tttttctatt tcttcaaaac aataataggg ctgtttttct tctaagatat
      901 cggtaataaa tgcaaaaaca aagggggaat gaacgccatg tcctttacga tgttttgcat
      961 atattttata gcgaaaaaat tgaaagatat gccatatttt ttgttgccac atagttggta
     1021 ttgggtctta ggtttaatta tacttgttta ttcctctgac gtatcatcat cgtattgttg
     1081 gtacaaaaag tcgttgtatg ggaatcgggt ggtatgaatt tcttttgctc gttcgtatag
     1141 caagtctttt aatgtttcta tatttgtttt ttcttttgca gaaataaata tacagttttg
     1201 ttttagtttt gacatccacg tttcctgtag ttcttgcagt gaaatatttt cttttcgtaa
     1261 gggggttaaa tcatcttcat cttttttttc gtacgtaaac gcatcaattt tgttaaagac
     1321 aagaatggtt aatttttctt tgttatcaat ttcatgcagt gttttttcta ctacttcaat
     1381 ttgttcttca aatgcaggat gagaaatatc tacgatgtgt actagtatat cggcttctct
     1441 tacttcatct agagttgact tgaatgattc gactaagcca tgaggcaatt ttctgataaa
     1501 acctactgtg tcagatagta gaaatgcgag attgttaatc gtaatttttc taacggtagt
     1561 gtcgagagtg gcgaataatt tattttcggc aaatacattt gatttactga gcaagttcat
     1621 taaagttgat ttgcctacat ttgtgtaacc aactaatgct actctaacca ttttgccacg
     1681 atttttgcgt tgaatggttt tttgtttatc tatatggatt aattctgctt taagtctagc
     1741 tattttgtct aatattatac gtctgtctgt ttcaatttga gtttcacccg gacctcttaa
     1801 tccgatacct cctttttggc gttcgaggtg agtccataaa cgtgttaatc ggggtaagaa
     1861 atattcgtat tgagcgagtt caacttgtgt ttttgcgtga gctgtttgtg ctctttttgc
     1921 aaaaatatct aaaatcagac ttgttctgtc tagggcttta gcttctagtg tgttttcgat
     1981 attgcggagt tgagaagggg ataattcatc atcaaaaata acgaggttga tttcttgttc
     2041 ttgtataaag ttttttattt cgagtagttt tccttttccc acataggttt ttgtatttgg
     2101 gagctctact cgttggtaaa actcttttat gacaatagct ccagcagttt ctgccaaaaa
     2161 cgcgagttct ttgagatact cttttgattt tgtttcattt tgttgttggg ttattagacc
     2221 aactaatact acgcgttcaa tatgtatttc agaatgagaa atcatagctt atataggtta
     2281 aaaaagcctc accaaatcgg taaggctttg tttttgtact atctatccta ttcggaaccg
     2341 aattggtaat gtgtatttta cacgcacatt tttacctcct tgtcgtcctg gattccatgg
     2401 tggcattgct tttactacac gaacagcttc tttgtctaaa ctttcttcta ctccgcgtac
     2461 aacttctacg tccacaattt taccgtctgt atttaccaca aattgcacta ctacacgccc
     2521 ttgtattccg ttttcttgag caataacagg gtagcgaatg gttcggttga tgaattgatt
     2581 catagcagcc tctccaccag ggaactctgg catacgctct actactacgt gtattctact
     2641 ttcttcagga tcttctactg gtgctgcaat aggagcttga attactactt ttttgttttt
     2701 atcatcttca gagttgaaat tttttgtctg aatatcctct ttgtcatcac gaatatcaat
     2761 gatatcactt aaaacggttt cttggatagg tggcgctggt ggtggcgggg tttcatcacg
     2821 gaaagttacc tcaatcatct cttcgtcttc tacaaattct ccttgaattg catcctcata
     2881 cactttgatt tcatttttag accattcgaa tgcaatgaac ataattccaa gggctagaac
     2941 taaaccaatc agaattgcca ttgttttttt gttttccaaa ttggcttttg gggtcttttt
     3001 tatttccata tacgttgaag tttgtcgttt acacagttgc ttgcaaatgt acaatatttt
     3061 tttttaatag cttgtatatc ctgtaatttt ttactgatat tttttttgaa cttgattttg
     3121 ttgcttagta acatcgttat ttaccacaaa agatgtatat ttgaatgttt tttttagtat
     3181 aacgaaatga aggtaagaat taatatattt atgtcggttg ttttactgtt tagtacctac
     3241 atacaggcac aatcgctttt tactatgagt tttttagagc aaccggtttc tgctcatcat
     3301 gctgctttag gagggaataa tacatcttat ttggggcatg atatttcttt tttagtatcc
     3361 aatccggcgt tattgtctat tcaatcatcc aacgagttgt ctgtgaatgt ggcaagttat
     3421 tttgctgggt caggttatgg ttcaggaata tatagtcgtt tgttacatga gcgaggcatg
     3481 ttaggggcgg gatttcagta tatggggtat ggagagttta ctggatttga tgaatatggt
     3541 gtagaaacgg ggacgttttc ggcacgagat gttgcgttct ttgtgagtta ttcacaacag
     3601 ttgaataatt attttagtat tggagctacg gttaagcctg tgttgggggt ttacgaacgc
     3661 tatgtgtcat atgcattggc tggggatatg gggatagttt ttatggacaa ggaaaatggc
     3721 ttgcaggttg ggttctctgc caaaaatatt ggagtaagat ttgctgtgta tgtatctgat
     3781 gaatcagctt ctatgttgcc cttaaatttg agtcttggag cctcaaaaaa gctattgcat
     3841 gctccttttg tgtttcatct aacaatgcaa cagttacaac gttggacgta tgatgtgtct
     3901 acaaatgttg attctacatc catatctttt gtgggggagt tggctcgcca ttttattgtg
     3961 ggggtagatg tattgcctcc ttccgataag ttttggctag cattatcgta taatttttta
     4021 cgcggacagg aacttcctgt tcctgaaata ttttctttga gtggtttttc tggcggtatt
     4081 ggttttttta taaaggatgt tgagttaggc attggttttg gg
//
LOCUS       DAOSMY010000117         4118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21768_length_4118_cov_3.961359, whole genome shotgun sequence.
ACCESSION   DAOSMY010000117 DAOSMY010000000
VERSION     DAOSMY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21768_length_4118_cov_3.961359"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLD76_03660"
     CDS             <1..251
                     /locus_tag="PLD76_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOU27131.1"
                     /translation="VPGIYHFSNEGVCSWYDFTIAIHALYGISTCNVLPIESKEYPTK
                     APRPHYSVLNKQKIKSTFDITIPHWQTSLANCIGKLKT"
     gene            411..1928
                     /gene="atpD"
                     /locus_tag="PLD76_03665"
     CDS             411..1928
                     /gene="atpD"
                     /locus_tag="PLD76_03665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOU27132.1"
                     /translation="MSVKTARAYITQVIGPIVDIYFEEGGYALPEIHDALEIIKPSAK
                     NIIVECHQHIGERSVRTISMDSTDGLVRGMEVIPKNKPICMPIGNQIKGRLLNVIGDA
                     IDGIGELDRKGESPIHRDPPKYEDLTTTKEVLYTGIKVVDLLAPYSRGGKIGLFGGAG
                     VGKTVLIMELIHNIAKGYNGYSVFTGVGERTREGNDLLREMIESGVINYGEEFKKSME
                     EGDWDLSKIDHEALKTSMATLVFGQMNEPPGARASVALSGLTVAESFRDADASNAQGK
                     DILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGLLQERITSTKTGAITSV
                     QAIYVPADDLTDPAPATTFSHLDATTVLSRKIAELGIYPAVDPLDSTSRILEPNIVGE
                     LHYKTALRLVELLQRNKELQDIIAILGMEELSDEDKLIVHRSRRVQRFLSQPFHVAEQ
                     FTGIPGVFVPIEETIRGFNMIMDGEVDQYPESAFNLVGTIEDAIEKGKAILRGEN"
     gene            1935..2183
                     /locus_tag="PLD76_03670"
     CDS             1935..2183
                     /locus_tag="PLD76_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOU27133.1"
                     /translation="MIVEIYAPHKTLFSGNALAISLPGKMGKFAILDNHAPLVSVLGK
                     GNIYLTKKNEENISFSIDSGFVEVNNNIVKVCVEINKK"
     gene            2191..2565
                     /locus_tag="PLD76_03675"
     CDS             2191..2565
                     /locus_tag="PLD76_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27134.1"
                     /translation="MRKYYQIIVTSVIVSQLIIWLVFTFFLPEYWDTNFFLIPIYFLA
                     IEIVFCKLLERFYVAKQKLNWYVIFKLGKLLLSIILAALYILAIQTQAIAFLLKFGVV
                     YLVFLFVETWIFLDYQKAQKGK"
     gene            2562..3644
                     /gene="atpB"
                     /locus_tag="PLD76_03680"
     CDS             2562..3644
                     /gene="atpB"
                     /locus_tag="PLD76_03680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOU27135.1"
                     /translation="MNQYFKYILLCFTLLFVVSYTQAANNHKEDEKPFDANTFIFGHI
                     ADAYDFHITSVGHKHISVPLLVIVKGKENGWSVFPSYRITHGDTYKGYYIQKKGSYKG
                     KIVETLSNGEIHRPFDLSITKNVFSILLSIFILIALFVPAANAYKKNPLGVPTKKNHL
                     LEMVILSIQNDVVKPSVGKDYAKYSPYLLTVFFFILITNLMGLIPFFPFGANVTGNIA
                     VTMVLAIFTYLITNIFGSREYWKEIFWPDVPVMLKFPLPLMPLIELISTFTKPFALLV
                     RLFANIFAGHMIILVLMALIFIFGSINPGVGYGVSIISIGFSIFMFMLELLVAFIQAY
                     VFTTLSAIFIGLSRVEPHHHPKKEIN"
     gene            3676..3930
                     /gene="atpE"
                     /locus_tag="PLD76_03685"
     CDS             3676..3930
                     /gene="atpE"
                     /locus_tag="PLD76_03685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOU27136.1"
                     /translation="MLLSIILQAVASASLAKLGASLGAALAVVGAGLGIGKIGSSAME
                     AIARQPEAASDIRSSMIVSAALIEGATLFAIIVCALAILI"
     gene            complement(3923..>4118)
                     /locus_tag="PLD76_03690"
     CDS             complement(3923..>4118)
                     /locus_tag="PLD76_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27137.1"
                     /translation="SDLIMYFSRSLIDFKIIGNPNFDKIKKTIPKAMVIQITNPDSGR
                     NKSIRLFIKFKGIYSGTLLK"
BASE COUNT         1340 a          677 c          735 g         1366 t
ORIGIN      
        1 acgtgcctgg catatatcat ttctctaacg aaggcgtatg tagttggtac gacttcacaa
       61 ttgctataca tgctttatat ggcatttcta cctgtaatgt gttacctatt gaaagtaaag
      121 aatatccaac caaagctccc cgtccgcatt atagtgtcct taacaaacag aaaataaagt
      181 ccacttttga cattactatt cctcattggc aaacatcgtt agccaattgt ataggtaaat
      241 taaagacgta ataatcgcga caccttgctc aaagtaagca cgttttacct tggcaaataa
      301 aataaaaaaa gaatgttaaa taatttgttt agttcttttt ttacagtaac tttgggcgtt
      361 ctttttacaa ttaacgagaa acactaacct ttattctaca aacaaatact atgtcagtta
      421 agactgcaag agcttatatt acacaggtaa ttggacctat tgtggatatc tattttgaag
      481 aaggtggata tgcccttcct gaaatccatg atgcattaga aataatcaaa ccttcagcta
      541 aaaacattat tgttgagtgc caccaacaca ttggtgagcg ttctgtgcgt accatatcaa
      601 tggattcgac agatggtcta gtaagaggca tggaagttat ccctaaaaac aagccgatct
      661 gtatgcctat tggtaatcaa ataaaaggac gattacttaa cgtaattggc gatgccatag
      721 atggaatagg cgaacttgat agaaaaggag aatcccctat ccacagagac cctcccaaat
      781 acgaagattt aacgacaaca aaagaagtac tatatacagg tattaaagtt gtagatttac
      841 ttgctcccta ctcacgtggg ggtaagattg gactctttgg aggtgctggt gtaggtaaaa
      901 cagtacttat tatggaatta atccataata ttgcaaaagg atacaatggt tattctgttt
      961 ttactggcgt aggcgaacgt acacgcgaag gaaatgattt attgcgagaa atgattgaat
     1021 ctggcgtaat caattatgga gaagaattca aaaaaagcat ggaagaaggc gattgggatt
     1081 tatccaaaat tgaccatgaa gccttaaaaa cctcaatggc tacgctcgta tttggacaaa
     1141 tgaatgaacc tcctggagca agagcctctg tggcactttc tggattaacg gtagctgaat
     1201 catttcgtga tgccgatgca agcaatgctc aaggaaaaga tattttattt tttatagaca
     1261 atatcttccg ttttacacaa gcaggttcag aggtttctgc actactaggt agaatgccgt
     1321 ccgctgtagg atatcaacct accttagctt cagaaatggg attgctacaa gagcgtatta
     1381 cttcgactaa aacaggtgca atcacttctg ttcaagccat ttacgttcct gcagatgact
     1441 taactgaccc agccccggca accacattct ctcacttaga tgcaacaact gttttaagta
     1501 gaaaaattgc agagctagga atctaccccg cagtagaccc tcttgattca acatcacgta
     1561 ttttggaacc aaatattgta ggcgaattac attacaaaac tgcattaagg ctagttgagc
     1621 tattacaacg caacaaagag ttacaagaca ttattgctat tttaggaatg gaagaattat
     1681 ctgatgaaga taaattaatt gtacatcgtt caagaagagt acaacgattt ttatctcaac
     1741 catttcacgt tgctgaacaa tttacaggga tacccggtgt ttttgtacca atagaagaaa
     1801 ctattcgtgg gtttaatatg attatggatg gagaagttga ccaataccct gagtctgctt
     1861 tcaacttagt aggcaccata gaggatgcaa tagaaaaagg gaaagctatt ttgaggggag
     1921 aaaactaaat acatatgata gtagaaattt atgcaccaca taaaacactt ttttctggca
     1981 atgcattagc aatatcttta cctggaaaaa tgggtaagtt tgcgatactt gataaccatg
     2041 ctccactggt ttctgtacta ggaaaaggaa atatctatct cacaaaaaag aatgaagaaa
     2101 atatttcttt tagtattgat agcggttttg tagaggtaaa taataatata gtaaaagtct
     2161 gtgtagaaat taataaaaaa taaatatagc atgagaaaat attaccaaat cattgttacg
     2221 agtgtaattg tatctcaact aataatttgg ttggtattta cctttttttt acctgaatat
     2281 tgggatacca atttttttct tatccctatt tattttttag caattgagat agtgttttgt
     2341 aaattattgg aacggtttta tgtagccaaa caaaaattaa attggtatgt tatttttaag
     2401 ttaggaaagt tattattatc aattattctt gcagctcttt atatacttgc aattcaaaca
     2461 caagcaatcg catttttatt aaaatttggt gtggtatacc tagtgttttt atttgttgag
     2521 acttggatat tcttagatta ccaaaaagca caaaaaggaa aatgaatcag tatttcaaat
     2581 acatattgct atgttttact ctcttgtttg ttgttagcta cacgcaagca gcgaataacc
     2641 ataaagaaga tgagaaaccc tttgatgcca atacatttat ttttggacat atagcagatg
     2701 cttacgactt tcatattacg tcagtaggac ataagcatat ttctgtacct ttattagtaa
     2761 ttgtaaaagg gaaagaaaat gggtggtctg tttttccttc ctacagaata acacatggag
     2821 atacctacaa gggttattat atccaaaaaa aaggaagtta taaagggaaa attgtagaga
     2881 cactatccaa cggagaaata catagacctt ttgatttatc gataaccaag aatgtatttt
     2941 ctattttact ttctatcttt atattaattg ctctatttgt tcctgcagcc aatgcatata
     3001 aaaagaatcc tttgggtgtt cctactaaaa aaaatcattt attagaaatg gtcattctat
     3061 caatccaaaa tgacgtagtg aaaccctcag taggaaagga ttacgcaaaa tactcaccct
     3121 acttgcttac agtttttttc tttattctta taaccaattt aatgggcctt attccatttt
     3181 ttccttttgg ggcgaatgta actggcaata ttgccgttac catggtattg gctattttca
     3241 cctatcttat tactaatatt tttggatctc gcgaatattg gaaagaaatt ttttggccag
     3301 atgtgcctgt tatgctgaaa ttccctttac ctttaatgcc tttaatagag ttaatatcta
     3361 catttacaaa gccatttgct ttacttgtgc gtttgtttgc taacatattt gcaggtcata
     3421 tgattatttt ggtattaatg gctttaattt ttatttttgg tagcatcaat ccaggcgttg
     3481 gatatggagt ttctattatt tcaatcggat tctccatatt tatgtttatg ctcgaattac
     3541 tggtggcttt tatacaagcg tatgtattta ctactttgtc agctattttt ataggtttat
     3601 ctcgagtaga acctcatcat catcctaaaa aagaaataaa ttaaaataaa tcattcactt
     3661 aaaacaaaaa aaactatgtt attatcaatt attcttcaag cagtagcatc agcctctttg
     3721 gcaaaattag gagcaagttt aggtgcagca ttagctgtag ttggagctgg tttaggtatc
     3781 ggtaaaattg gtagctcggc aatggaagcc attgctcgtc aaccagaagc tgctagtgat
     3841 attcgttcaa gtatgattgt gtcagcagct cttattgaag gggctacttt atttgcaatt
     3901 atcgtatgtg ctttggctat tcttatttaa gtaaagtgcc actataaata cctttaaatt
     3961 taataaatag tcttatggat ttatttcttc cggaatcagg attggtaatt tggatgacca
     4021 tagcctttgg tatcgtcttt tttattttgt caaaatttgg gttccctatt attttgaaat
     4081 ctattaatga acgagaaaag tacattatca aatcgctt
//
LOCUS       DAOSMY010000118         4114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21796_length_4114_cov_4.617147, whole genome shotgun sequence.
ACCESSION   DAOSMY010000118 DAOSMY010000000
VERSION     DAOSMY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21796_length_4114_cov_4.617147"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2027
                     /locus_tag="PLD76_03695"
     CDS             <1..2027
                     /locus_tag="PLD76_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU27138.1"
                     /translation="LLCGFGFLPSNVSMAQNIYLETIDRIVENLEMSDDEVVLDDTYE
                     DLLRLAENPLNVNTCTREQLEQLFFLSPYQIENLLYYRYSYGDLYTVYELQLIDGFDE
                     ETIQFLLPFIYVRKSELQSEGITLQNAFRYGKNQVLLRTDCTIQKKKGYTHLATDAQR
                     YVGNPLYAQFRYRFSSQYLSIGVSMEKDAGEPFDWHYNKGFDFYAPYLFISSLWKFKH
                     IALGHYKLNFGQGLVLQSSQRFGKSSLFSNTIYKEGISKSASLDESLYFNGLATTIGH
                     KKLEITAFYSNTPLDGTLEYDSNNLLGLSSIKTDGIHRTENDFVKKNVARQMVIGGNI
                     QYHFPFLKLGITGFSYSLDKPSFPSSKPYAIYEFAGKHYSTASIDYRARFHQFNVAGE
                     MAIDKNGAMATVHTLSFQPISRFGLAALYRYYQPEYSSIFGNGFSQSSSTNNEEGFFM
                     GTELLPFRKWKLVSYLDVFSFPWLKYLVNTPSRGYQGFIQATYTPTRNTTFFCQYKST
                     SKQRNVLLEEMATPMVNPYTKNEWRFQIDTNPFKHLHCKTGMHSNEYASQLNHPTYGF
                     LLYQDVGYEFSSIPLLLNARYALFDAINYDNRLYAYERDVLYAFSVPMYYGQGMRLYV
                     NVVYKPFPQLTWYAKFAHTWYADRNEIGSEGETIYGNQKTDIRILVNWKF"
     gene            2081..2629
                     /locus_tag="PLD76_03700"
     CDS             2081..2629
                     /locus_tag="PLD76_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU27139.1"
                     /translation="MNSFYNLVSSRQSDRAFLPKGIEDEKLQRILASAQLAPSACNAQ
                     PWHFVVVNETDKRLQIADAMASKVLAMNHFTKQAPVQIVVVEEKANISSSFGSWAKSK
                     HFPHIDIGIVASHICLAAADEGLGTCMIGWFDEKKIKNILSIPDEKRVLLVIVMGYSA
                     QETRPKKRKKLNEIVSYNSYKK"
     gene            complement(2636..3481)
                     /locus_tag="PLD76_03705"
     CDS             complement(2636..3481)
                     /locus_tag="PLD76_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27140.1"
                     /translation="MKRLLIALILTCIPAHYVFSYTLTGWFEESKISVFGGVATHHIE
                     KRNSTEDVNLMNYLSYRNEFYLGVDAHVYLHEQWQFNIKTYLNSSLRTDDVFAKLIYM
                     PHEKIGVTLGYASIPVQYGLYEIHEQPQYYTTIPNSDDVYDFAKNELFLAGLAFPFEG
                     GKFSGTIYLQGGVSYLPNDFSFSWQNKYDSNERRLVEYDIPESYAFIIQPEVEIRWEI
                     AKPTTDTSIGLQFQGKWNYSKRNIDYTRNIYTWTTDNKQTEIISHPKQSIEGVDWNVG
                     LFYIF"
     gene            complement(3486..>4114)
                     /locus_tag="PLD76_03710"
     CDS             complement(3486..>4114)
                     /locus_tag="PLD76_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27141.1"
                     /translation="MNRIYKISSLLIFLFIINLIQSCGPSMDGYIGFDITHIQIQNLS
                     NTGSDAVVTTENTMLPSAVAFKVRLYEGSNSVGLRMQPQTNFGFSSCVALSPPDFYYK
                     SNQLIQNIRVVTLVDYSTSIVANTDVSDKFLVYPIVPAVSDFLYVSISEFCTLTTDQI
                     LMSPSIEFHLMCKETLSLNNAQFRIIVELSNGQELSIDTALLDLVTND"
BASE COUNT         1292 a          702 c          770 g         1350 t
ORIGIN      
        1 tcttgctttg tgggtttgga tttttaccct ccaacgttag tatggctcag aatatttatt
       61 tagaaacaat agatagaatc gtagaaaact tagaaatgag tgatgatgaa gttgttttag
      121 atgatacata cgaggatttg cttcgattgg cagaaaaccc actgaatgta aatacatgca
      181 ctagagaaca attagaacaa ttattttttc tctctccata tcaaattgaa aatttactgt
      241 attatcgcta ttcttatggc gatttgtaca ctgtatacga gttacaattg attgatggtt
      301 ttgatgagga aacgattcaa tttttattac cttttatata tgtaagaaag agcgaactac
      361 aatcagaggg cataacgtta cagaatgctt ttcgatatgg aaaaaatcaa gtattgttgc
      421 gaacggactg tacaatacaa aagaaaaaag gctatacgca tcttgccaca gacgcacaac
      481 gatatgtggg gaatccatta tatgcacaat ttcgttatcg cttttcgtca cagtatttat
      541 ctatcggagt aagtatggaa aaagatgcag gagagccttt tgattggcac tataacaagg
      601 gttttgattt ttatgcacca tatttgttta ttagttcact ctggaagttt aaacatattg
      661 cactaggaca ttataaactt aattttggtc aaggattagt gttacagtca agtcaacgat
      721 ttggtaagtc ttctttattt tctaacacga tatacaaaga aggaataagt aagagtgcct
      781 cgctggatga atctctgtat ttcaatggat tagcaacaac tattggtcat aaaaaattag
      841 aaataacagc cttttattca aatactcctc ttgatggaac acttgaatat gattcgaaca
      901 atctattggg cttgtcatca ataaaaacag atggaataca tcgtacggaa aatgattttg
      961 tgaaaaagaa tgtagcccgt cagatggtga ttggtggaaa tattcaatac cacttcccgt
     1021 ttttaaaact aggtattact ggtttttctt attctcttga taagccgtct ttcccctcat
     1081 ccaaaccata tgctatatac gagtttgctg gcaaacacta ttcaactgct agtattgatt
     1141 atcgggctcg ttttcatcaa ttcaatgtag ctggcgaaat ggctatagat aaaaatggtg
     1201 ctatggctac tgttcatacc ctttcttttc agcctatttc tcgttttgga ttggcagctt
     1261 tatatcgcta ttaccagcct gagtatagta gtatatttgg taatggtttt tcacaaagta
     1321 gtagtacaaa taacgaagaa ggctttttta tgggtacaga attattgcca tttcgtaaat
     1381 ggaaactggt ttcttatcta gatgtgtttt cttttccgtg gttaaagtat ttagttaata
     1441 ccccttctcg tggttatcaa ggctttattc aagcaacata tacgccgact cgaaatacca
     1501 catttttttg tcaatataaa tcaacatcca aacaacggaa tgtgttactc gaagagatgg
     1561 caacaccaat ggtaaatcca tatacaaaaa atgagtggcg atttcaaatt gatactaacc
     1621 cttttaaaca tttacattgt aaaacaggta tgcattccaa tgagtatgcc tctcaactca
     1681 atcatcccac ttatggtttt ctcttatatc aagatgttgg ctacgaattt tctagtatcc
     1741 ctttgttgtt aaatgctcgt tatgctttgt ttgatgccat taactatgat aatcgtttgt
     1801 atgcatacga aagagatgtt ttatatgctt tttctgtgcc gatgtattat ggacaaggaa
     1861 tgcggttata tgtaaatgtt gtttataagc ctttccctca acttacttgg tatgcaaaat
     1921 ttgcacatac ttggtatgcc gacagaaatg agataggctc tgaaggagaa actatttatg
     1981 gaaatcaaaa aacagatatc cgcattttag tgaattggaa gttttaaacg aattttacca
     2041 gaaaaatttc tatttttgta caatactaat agtaatgctt atgaattcct tttataatct
     2101 tgtctcttcc cgacaaagcg atagagcctt tttgccaaaa ggtattgaag atgaaaaatt
     2161 acagcggata ctcgcctctg ctcagttggc tccttcggca tgcaatgcac aaccttggca
     2221 ttttgtggtg gtaaatgaaa cagataagcg attgcaaata gctgatgcaa tggcgtctaa
     2281 agtcttggca atgaatcatt ttaccaaaca agcccctgtt cagattgtag tagtagaaga
     2341 aaaagcaaac atcagttcta gttttggcag ttgggcaaaa agtaaacatt ttccacatat
     2401 tgatattggg atagtagctt cccatatttg tttagctgca gcagacgaag gcttgggtac
     2461 ctgcatgatt gggtggtttg atgaaaagaa gattaagaat attctttcta tacctgatga
     2521 aaaaagggta ttgctagtaa tcgtaatggg ttattcagct caagaaactc gacccaaaaa
     2581 acgcaaaaaa ttgaacgaaa tagtgtcgta caatagctac aaaaaatagg gttttttaga
     2641 aaatataaaa caagccaacg ttccaatcta ccccttctat tgattgcttc ggatgcgaaa
     2701 ttatttccgt ttgcttgttg tctgtagtcc aagtgtatat gttacgtgta taatcgatat
     2761 ttcgtttcga ataattccat ttcccttgaa attgaagacc tatgctggta tctgttgttg
     2821 gttttgctat ttcccaacgt atttctactt cgggttgaat gataaatgca taactttcgg
     2881 ggatatcata ttctactaat ctacgttcgt tgctatcgta tttattttgc caagagaacg
     2941 aaaaatcgtt tggcagataa ctaactcctc cttgtaagta aatagttcct gagaatttac
     3001 ctccttcaaa aggaaatgca agacccgcta aaaacaattc gtttttagca aaatcgtata
     3061 catcatctga gtttggaatt gtagtataat actgtggctg ttcgtgtatc tcatataacc
     3121 catattgaac agggatagat gcatagccca aagtaacccc aattttttca tgtggcatat
     3181 agattagttt ggcaaacaca tcgtctgttc gcaaggagct gttcaggtat gttttgatgt
     3241 taaattgcca ttgttcgtgt aagtacacat gtgcatcaac gcctagataa aactcatttc
     3301 tatacgatag gtagttcatc agatttacat cttcagtgct atttcttttt tctatatgat
     3361 gcgtggcaac tcctccaaaa actgaaattt ttgattcttc aaaccatcca gtcagtgtat
     3421 agctgaatac gtaatgtgct ggaatgcaag taagaataag tgcaatgagt aatcgtttca
     3481 tgtgcttaat cgttagtaac taaatcaagt aatgctgtat caatactcaa ctcttgccca
     3541 ttcgataatt caacgattat tctaaattga gcgttgttga gagaaagtgt ttccttgcac
     3601 ataagatgaa attcaatact aggagacatc agtatttggt cggttgttag cgtgcagaat
     3661 tcggaaatgc tcacatacaa aaaatcagat acagctggta ctatcggata taccaaaaat
     3721 ttatccgaca catcggtatt ggctacaatg gacgtggaat aatccaccaa cgtaactact
     3781 cggatatttt gaatgagttg gttcgatttg tagtaaaaat ctggtggact aagggctaca
     3841 cacgaactga aaccgaagtt cgtttgtggt tgcatgcgta atccaacact attagagcct
     3901 tcgtaaagcc tcactttaaa agcaacagca ctcggaagca tggtattttc agttgtaacc
     3961 acagcatccg agccagtatt ggaaagattt tgaatctgta tgtgcgtaat atcaaagcct
     4021 atgtatccat ccatactggg accacagctc tgtatgagat tgatgatgaa caaaaagatg
     4081 agtaaggagc taattttata gattcggttc atat
//
LOCUS       DAOSMY010000119         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21809_length_4113_cov_3.671759, whole genome shotgun sequence.
ACCESSION   DAOSMY010000119 DAOSMY010000000
VERSION     DAOSMY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21809_length_4113_cov_3.671759"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PLD76_03715"
     CDS             <1..1280
                     /locus_tag="PLD76_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU27142.1"
                     /translation="VLSSGFSYMQMVLGFSVGYIIVAFVLLPLYYRLQLTSIYTYLYE
                     RFGKATHTTGSLFFLLSRTIGGATRLYIVALILQRFIFDTWNIPFFITVSLLLALIYL
                     YTYANGIKTIVWTDALQTLFLLIALILLIIQSMDFLSLDINTTVETIKNSSYTRLWIV
                     DDWKNPSHFIKLFISGVFITIAMTGLDQDMMQKNLSCSNTRSAQKNMLSYGMAFIPVN
                     ILFLTLGFLLVMITETQGIALPAQTDSLLPNIIADGILGSWAMVFFVIGIIAATFSSA
                     DSALVSLTTSFAVDIIHIEHMEEQKATTMRRWIHLAFCFVFLVIILLFHLINNKSILD
                     LLFTIASYTYGPLLGIFTYGIFTKWKISDTLVPYIAVISPVLAYGFFWVFSTYFNYYI
                     GYELLIINGTITFLGMLLFTKKKSLHPTKTIIL"
     gene            1277..1873
                     /gene="yihA"
                     /locus_tag="PLD76_03720"
     CDS             1277..1873
                     /gene="yihA"
                     /locus_tag="PLD76_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOU27143.1"
                     /translation="MIIQSATFVISNTDTSKCPNSQLPEYAFIGRSNVGKSSLINMLT
                     GVKNLAMTSSKPGKTLLINHFLINEQWHLVDLPGYGYARIGKQGRERLEEMIRVYITK
                     RVQLTNLFVLIDSRLEPQQIDIEFITWLGEEGIPFSLIFTKSDKLSKSALQLNISQYK
                     EKLYEVWEELPPIFVTSSEKGIGRDEVLQYIAEINASL"
     gene            1973..3466
                     /locus_tag="PLD76_03725"
     CDS             1973..3466
                     /locus_tag="PLD76_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU27144.1"
                     /translation="MLEIQKKMSNSFFALLSLPATAMGFALSVQISALSWLLSTKYGL
                     DIHEIGIVWAAGPIAGIIAQPIVGALSDKMWFMGGRRRPWIVIGGILAALMMLALPNL
                     EIIQKIFINSEDPQAGLLTIAVIVALTFDLSINVSFNPTRSIIADCTTMKDRSKGYTW
                     MQTISGSFGVLAYLIGGVLGNLFLIYFGVGIALLATLIPAFLIEEPRELAIEIDENEP
                     ITTAKTSKRDMIESLLPLSGFLLYGIYVIFEKLFGLHVTAGYTVFGYFLNVVELLCIL
                     LLVVSTIAVLFNLTKSNTKSNEFQRILLAHSFTWIGVQTMFVYAYFYLKEIILLEDKV
                     AGSVNSIAFFILSLVSAILPVLVLNKFAEKIGMVKTHLACIFIMALGYAGVVLFGTSV
                     IPYYVMLGVVGIGWASVVSLPFAIMSSKVDQTKMGLYMGIFNLSVVLPQLVASFKMGE
                     VISGSDDKSIIFVICAITLFISGVLWFFVKEAKVSKTANAPKGGGGH"
     gene            complement(3806..>4113)
                     /locus_tag="PLD76_03730"
     CDS             complement(3806..>4113)
                     /locus_tag="PLD76_03730"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOU27145.1"
                     /translation="LSLEDIPYYTLFPTDIDPSFNCRKSTTITEKTICRDAELANLDR
                     ELFLLYKKIKKNETIIQSQRNWIKNREEQIEKLDTHEERVKLIKSLYLDRIEELQNF"
BASE COUNT         1223 a          671 c          741 g         1478 t
ORIGIN      
        1 gggtactatc atcaggattt agttatatgc aaatggtact tggcttttcg gtgggctata
       61 ttattgtggc ttttgtttta cttcctttat attaccgctt gcaacttaca agtatctata
      121 catacttata cgaacgtttt ggcaaagcaa cacacacaac aggctcatta tttttcttat
      181 tatcacgcac tattggtgga gctacacgtt tgtatatcgt tgctcttatt ttacaacgtt
      241 ttatttttga cacatggaat ataccttttt ttataacagt aagtttatta cttgctttga
      301 tttatcttta tacttacgca aacggcatta aaactattgt ttggacagat gctttacaaa
      361 ccttattttt actcattgcc cttattttac ttattattca atcgatggat tttttatctt
      421 tggatataaa cacaacagtt gagacaatta aaaatagctc ttacactcgc ttatggatag
      481 ttgatgattg gaaaaatccg tctcatttta tcaaattatt tatcagcggt gtatttatta
      541 ctattgccat gacaggactt gaccaagata tgatgcaaaa aaatctatct tgctcaaata
      601 ctagaagtgc tcaaaaaaat atgctcagtt atggtatggc ttttataccg gttaatatat
      661 tgtttttaac tttaggtttt ttattggtaa tgataactga aacacaaggt attgcactac
      721 cagctcaaac agactcttta ctgccaaaca tcatagccga tggcatattg ggctcgtggg
      781 ctatggtgtt ttttgttata ggcattattg ctgccacgtt ttctagtgcc gattcagcgt
      841 tggtatcatt aaccacctct tttgctgtag acatcataca tatagaacat atggaagaac
      901 aaaaagcaac cacaatgcgt cgttggattc accttgcttt ttgtttcgtt tttcttgtta
      961 ttattttgct ttttcatctc attaacaata aaagtatctt agatttatta ttcactattg
     1021 cctcctatac gtatggtccg ttattaggaa tatttacata cggaatattt actaaatgga
     1081 aaataagcga cacattagtt ccctatattg ctgtaatttc tccggtgtta gcgtatggat
     1141 ttttttgggt tttttccacc tatttcaact attatattgg atacgaactc ttgataataa
     1201 atggaacaat tactttttta ggaatgcttt tgttcactaa aaagaaatca ttacacccta
     1261 ctaaaacaat aatattatga ttatacaatc agctacattc gtaataagta atactgacac
     1321 cagtaaatgc cccaacagtc aactgcccga atatgccttt attggtcgtt ctaatgtagg
     1381 caaatcatcg cttatcaaca tgcttactgg ggtgaaaaac ttggcaatga cgagctccaa
     1441 accaggaaag acattgctta tcaaccattt tcttatcaat gaacagtggc atttggtcga
     1501 cttaccagga tatggttatg cccgtattgg caaacaaggc agagaacgat tagaagagat
     1561 gatacgtgtg tacattacaa aacgcgtgca acttaccaat ttatttgtat taatagattc
     1621 tcgactcgaa ccacaacaaa ttgatattga atttattaca tggttaggcg aagaaggtat
     1681 tccgtttagt cttattttta cgaaatcaga caagctatca aaatctgcac tacaactaaa
     1741 tattagtcaa tacaaagaga aactatacga ggtatgggaa gagcttccac ctatttttgt
     1801 tacttcatca gaaaaaggca ttggacggga cgaagtgcta caatatattg cggagataaa
     1861 tgctagcttg tagttttttt tagttaaaac tctttgtgat tcaaaatttc cttgtatttt
     1921 tgttccgatt ttgcagaaga aaatcattat ctataaatta aaaataaata ctatgttaga
     1981 aattcaaaag aaaatgagca attcattctt tgctcttctc agtcttccag ccacagctat
     2041 gggatttgcc ttatcggtac aaatctctgc attaagttgg ttactttcca ccaagtatgg
     2101 cttagatatt cacgaaattg gtattgtatg ggcagcagga cctattgcag gtattattgc
     2161 acaacccatt gtaggtgcac tgagtgataa aatgtggttt atgggtggtc gtcgtcgtcc
     2221 ttggattgtg attggaggta ttttagcagc attgatgatg cttgcattac caaacttaga
     2281 aattattcaa aaaatattta tcaatagcga agaccctcaa gctggtttgt taactatagc
     2341 cgtaatcgta gctttaactt tcgatttaag tatcaacgta agttttaacc ctacccgctc
     2401 tataattgct gattgtacta cgatgaaaga tcgctccaaa ggatacacct ggatgcaaac
     2461 tatttcagga tcatttggcg tattggctta tttaattgga ggtgtattag gcaacttatt
     2521 tttaatctat tttggggttg gtattgcgtt attagcaact ttaattccag catttctgat
     2581 agaagaacct agagaattgg caatagaaat tgacgaaaac gaacctatta ctacagccaa
     2641 aacatccaaa agagatatga tagaatcatt gctcccctta tctggatttt tgttgtatgg
     2701 aatttatgta atttttgaaa aactatttgg attgcatgta acagctggtt atactgtatt
     2761 tggctatttt ctgaatgtag tggaactatt gtgtattttg ttgttggttg taagcaccat
     2821 tgctgtgttg tttaacttaa ccaaaagcaa tactaaatca aacgaatttc aacgtatttt
     2881 gttggcacac tcctttacat ggattggagt acaaacaatg tttgtatatg cttactttta
     2941 cctgaaagaa attatcttat tagaagacaa agtagcagga agtgttaact ccattgcttt
     3001 ctttatacta agcttggtta gtgccatatt gcctgtgttg gtattaaaca aatttgccga
     3061 gaaaattggt atggtaaaaa cacaccttgc atgtatattt attatggcat tgggttatgc
     3121 tggtgttgtg ttgtttggca catcggtaat tccttactat gtaatgttag gtgttgtggg
     3181 tataggatgg gcatcggtag tgtcgttacc atttgctatt atgagctcaa aagttgacca
     3241 aacaaaaatg ggcttataca tgggtatttt taacctatca gtagtattgc cacagttagt
     3301 agctagtttc aaaatgggtg aagttatttc tggttctgat gacaaatcta tcatctttgt
     3361 tatttgtgct attaccttat ttatctctgg tgttttatgg ttttttgtaa aagaagccaa
     3421 agtatcaaaa acagcgaatg cccctaaagg tggcggtggt cattaattaa agtaacactc
     3481 tatatgcgaa aacagcgtaa cgaaatttgg ttacgctgtt ttttttatag ttacgatttg
     3541 caagttatag aaagcttaat attaaaatgc acttaacaag gtgtttagtg ttgttcgtcg
     3601 ttcgttaaac gttgttcgtt gttcgttgtt cgtgaggtag cggtagtttg gtttgcatag
     3661 ggaaagctgg agctttcctt ttaaattcga catagcgaga cgctatgccg aacacttaga
     3721 ttgtcagaga cgagggaacg ctatgcccga acacgcagat tataaaactt cacactacgg
     3781 cgaacaaagg gatgaagttt tattattaaa aattttgtaa ttcctcaatt ctatctaaat
     3841 atagactttt tatcaatttt actctttctt catgtgtgtc taacttttct atttgttctt
     3901 ctctgttttt tatccaattt ctttgagatt gaatgattgt ttcatttttt ttaatttttt
     3961 tatacagcaa aaacaattct ctgtctagat tagctaactc agcatctcta caaatggttt
     4021 tttcggtaat agtcgtagac tttctgcaat taaacgatgg gtcaatgtcc gttgggaata
     4081 atgtgtaata agggatatct tctaatgata aag
//
LOCUS       DAOSMY010000120         4105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21867_length_4105_cov_3.542222, whole genome shotgun sequence.
ACCESSION   DAOSMY010000120 DAOSMY010000000
VERSION     DAOSMY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21867_length_4105_cov_3.542222"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="PLD76_03735"
     CDS             <1..1532
                     /locus_tag="PLD76_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOU27146.1"
                     /translation="YIEQFIAPLKQKIIDAGFDFDIKGRTKSIYSIWNKIKKQDTPFE
                     NIYDLFAIRIILNSDITKEKADCWQVYSIVADMYQPNPKRLRDWLSIPKSNGYESLHT
                     TVMGPQGKWVEVQIRTKRMDEIAEKGLAAHWKYKGIQGESGMDEWLKNIREILENPEL
                     NAIDFMDEFKLNLYDKEVFVFTPNGDLQKLPKGATLLDFAFHIHTGLGTKCVGGKANG
                     KNVTLKYVLKNGDQIEIQTSATQKPKLEWLNIVTTSKAKNKIRQAVKDEQFKDAQMGR
                     EILQRRFKNWKIEVEESKISRLAKKKGFKTVSDFYQELAQEKLDILSLRDAYIDLDKR
                     ETTEPSDYRSAENFVQDNTLQDTTSKEDVLIIDQNLRGIEYSLAKCCNPIFGDDVFGF
                     ITTSGGFKIHKKDCPNAPQLISRFGYRIIPARWSGKSGTSQYAITLGVIGVDDIGIVT
                     NISSLLSKEKNITLRSISVNSHDGLFEGRITIMVGELQELENIIKKIKTVKGVKSVQR
                     I"
     gene            1586..2242
                     /locus_tag="PLD76_03740"
     CDS             1586..2242
                     /locus_tag="PLD76_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU27147.1"
                     /translation="MLHTNPCNKTILFDFDGTLANTLKTVVEIYNKVAPEYNCKTVEL
                     EDVSRLQAMTISHLMKEQGISHITLAILLVRVRKELHINIDHVKPFFGIEEQLQTLKR
                     MGYQLGIMSSNSQKNVHTFLESNNMKHLFDFVHTSKNIFGKHTAIKRIISNLSLQTND
                     VVYVGDETRDIEACKKIGVKIAAVSWGYNLPEVLQAMQPDVLIDEPHSLANCISKLAM
                     "
     gene            complement(2247..3512)
                     /locus_tag="PLD76_03745"
     CDS             complement(2247..3512)
                     /locus_tag="PLD76_03745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU27148.1"
                     /translation="MTFEQLNLVEPILNALKHEGYTTPTPIQEVSIPIILKGTDLLGC
                     AQTGTGKTAAFAIPILQHLYLDKGSDRAPRKIKVLIVTPTRELAIQIGESFAAYGRFM
                     DVRHTVIFGGVNQQSQVNALKNGVDILVATPGRLLDLQTQGYIKLKDLTTFVLDEADR
                     MLDMGFIHDINKILKLIPAKRQTLFFSATMPNPIRVLANTMLTKPEEVSVTPVSTTAE
                     TIQQEVYFVDAANKRELLVHVLQDKKIDTALVFTRTKHGADKVQRMLSKAKIQTEAIH
                     GNKSQNARQNALRNFKDRTTRVLVATDIAARGIDIDALNLVINFDIPNIPETYVHRIG
                     RTGRAGLDGKAVSFCDVEEIPYLRDIHKLIGMQIPVVSDHPFPSSNVLTEVVDNKNRA
                     KKKPTIQPSKGKRRPFVSNKPTLVKQPRG"
     gene            3813..>4105
                     /locus_tag="PLD76_03750"
     CDS             3813..>4105
                     /locus_tag="PLD76_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOU27149.1"
                     /translation="MFSGIIEEAAKIVQLDKDQENVHITVECSFVNELKIDQSVAHNG
                     VCLTVVKKTPTTYTVTAIKETLERSNLGQLTVGDKINLERSMLMNGRLDGHIV"
BASE COUNT         1428 a          719 c          762 g         1196 t
ORIGIN      
        1 gctatattga gcaattcatt gccccattga aacaaaaaat aatcgatgct ggttttgatt
       61 ttgatataaa aggaagaaca aaatctattt acagtatttg gaataaaata aaaaaacaag
      121 atacgccttt tgaaaacatt tatgatttgt ttgctattcg tattattctc aacagcgata
      181 taaccaaaga aaaagccgat tgttggcaag tatattcaat agtagcagac atgtaccaac
      241 ccaatccaaa acgtttacgc gactggctat ccatacccaa aagcaatggg tacgaatcgc
      301 tacatactac ggtaatggga ccacaaggca aatgggtaga agtacaaata cgtaccaaac
      361 gcatggatga aatagcagaa aaaggcttag cagcacactg gaaatacaag ggtatacaag
      421 gagaatcggg gatggacgag tggctgaaaa acattcgtga aatattggaa aatcctgaat
      481 tgaatgcaat tgactttatg gatgagttta agctcaactt atacgacaaa gaagtatttg
      541 tatttacacc caatggcgat ttgcaaaaac taccgaaggg agctactcta cttgactttg
      601 cctttcatat tcatacgggt ttgggaacaa aatgtgtggg aggaaaagcg aatggaaaaa
      661 atgttacttt aaaatatgta ctaaaaaatg gagaccaaat agaaatacaa acctcagcta
      721 ctcaaaaacc aaaattagaa tggttgaata tcgtaaccac ctctaaggca aaaaataaaa
      781 tccgtcaagc tgttaaagac gaacagttta aagacgctca gatgggtaga gaaattttgc
      841 aacgcaggtt taaaaactgg aaaattgaag tagaagaatc aaaaatatcg cgtttggcta
      901 agaaaaaagg gtttaaaaca gtaagtgatt tttaccaaga attagcacaa gaaaaattgg
      961 acatcctaag cctacgtgat gcttatatag acttagataa aagggaaacg acagaacctt
     1021 cagattatcg gagtgctgaa aattttgtac aagataatac attgcaagat actactagta
     1081 aagaagatgt attgataatt gaccaaaact tgcgtggaat cgaatattca ttagcaaaat
     1141 gttgtaaccc aatttttggc gatgacgtgt ttggctttat tacgactagt ggtggtttta
     1201 aaattcataa gaaagactgt ccaaatgcac ctcaactcat ttcacgtttt ggatacagaa
     1261 ttatccccgc tcgttggtca ggcaaatcgg gtacttcgca atatgccatt acactcggag
     1321 taatcggggt tgacgatatt ggtattgtaa ccaacataag ttcgttgtta tcaaaagaaa
     1381 aaaatattac cttacgttct atttcggtaa attcgcatga tggacttttt gagggaagaa
     1441 ttaccattat ggtaggagag ttacaagaat tagagaatat tataaaaaaa ataaaaacag
     1501 taaaaggcgt taaaagtgtt caacgtatat aaaataggca ttttttattc cctgacaatt
     1561 aatttttata caatttattc atattatgct tcatacaaat ccttgcaata aaaccatttt
     1621 gttcgatttt gacggaacct tggcaaacac cctgaaaaca gtagtagaaa tttataacaa
     1681 agttgctccc gaatacaatt gtaaaacggt agaattagaa gatgtgtcga ggctacaagc
     1741 gatgacaata tcgcatttaa tgaaagaaca aggcatctca catatcactt tagctatttt
     1801 gttggtacga gtacgcaagg aattacatat caacatcgac cacgtgaaac cattctttgg
     1861 catcgaagaa caactacaaa cattaaaaag aatgggttat caattaggaa ttatgtcttc
     1921 aaactctcaa aaaaatgttc acactttttt agaatcaaac aacatgaaac acctatttga
     1981 tttcgttcac acaagtaaaa atatttttgg caaacacact gctattaaac gtattatatc
     2041 aaacttatcg ctacaaacaa atgatgtagt atatgtaggt gacgaaactc gcgacataga
     2101 agcttgcaaa aaaattggtg taaaaatagc ggcagttagt tggggctata atttacccga
     2161 agtattacaa gctatgcaac ctgatgtact tattgacgaa ccacatagcc tagcaaactg
     2221 tatatctaaa ctcgctatgt aatgccttat cctctgggct gtttaaccaa ggtaggttta
     2281 ttgcttacaa acggacgcct tttgcctttt gaaggctgta tggtaggttt ctttttcgct
     2341 cgatttttgt tgtcaacaac ttctgttaat acattgctac taggaaacgg atgatccgaa
     2401 acaacgggta tttgcatgcc tattaattta tgaatatcac gcaagtaagg tatttcttcc
     2461 acatcgcaaa aagaaaccgc tttgccatct aaccctgccc tacctgtacg cccaatacga
     2521 tgcacatagg tttcgggtat atttggaata tcaaaattga taaccaaatt aagggcatca
     2581 atatcaatgc cacgtgccgc aatatcagta gctactaata cacgcgtagt tctgtcttta
     2641 aaatttcgca aggcattctg acgtgcattt tgcgatttat tgccatgaat ggcttctgtt
     2701 tgtatttttg ctttcgatag catacgctgt actttatctg caccatgctt ggtacgggta
     2761 aatacaaggg ctgtatcaat ctttttatct tgcaatacat gtaccaataa ttctcttttg
     2821 ttggcggcat ctacaaaata tacttcttgc tgaatagttt cggcagtggt agatacagga
     2881 gtaaccgaaa cctcttcggg cttagttaac atagtattag ccaacacacg tataggatta
     2941 ggcatcgtag cagaaaaaaa caaggtttgg cgtttagctg gtattaattt cagtatcttg
     3001 ttaatatcat gaataaaacc catatccaac atacggtcag cctcatcgag cacaaatgta
     3061 gttaaatctt tcagtttaat gtaaccttgt gtttgcaaat cgagtaatct gccaggtgtt
     3121 gctaccaata tatctactcc gttttttaag gcattaacct gcgattgttg gttgacgcca
     3181 ccaaaaataa cggtatgacg cacatccata aagcgaccat aagccgcaaa actttcgcca
     3241 atctgaatgg ctaattctct ggtgggtgtt acaatcaaca cttttatttt gcgtggtgct
     3301 ctatccgagc ctttatctaa gtataaatgt tgtaaaatgg gaatagcaaa agccgctgtt
     3361 tttcctgtgc ctgtttgtgc acagcccaac aaatcggtac cttttagtat aatggggata
     3421 gaaacttctt gaatgggtgt gggcgtagtg taaccttcgt gttttagagc atttaaaatc
     3481 ggctctacaa gatttaattg ttcaaatgtc ataataaatt gggaatgtat tcattgttct
     3541 atcggtgtgt aaaattataa cctatatgaa tacaactggc gacaaagata gactaaataa
     3601 ctggttaaac tgttttttta gcaaaataat atcacttgaa gtgtgtatca acaagccatg
     3661 atgtatataa taaaacacta tttttaggta aacaatgggg tattctgtaa gcatactacc
     3721 tgctttcttg agatataaat attttcgaca cttgaaataa tacagtaaat tatctatctt
     3781 tgtaagcaaa taaacacatt aaactcacta ctatgttttc aggaataatt gaagaagccg
     3841 ccaaaatagt acaactggac aaagatcaag aaaatgtaca cattacagta gaatgctcgt
     3901 ttgtaaacga acttaaaatt gaccaaagtg tagctcataa cggcgtttgc cttacggtag
     3961 taaaaaaaac accaaccacc tataccgtta cagccatcaa agaaacgctt gaacgcagca
     4021 atttggggca gttaacagtg ggcgacaaaa ttaatctgga acgtagtatg ctgatgaacg
     4081 gtcgtttaga cggacacatc gtgca
//
LOCUS       DAOSMY010000121         4092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_21961_length_4092_cov_3.229874, whole genome shotgun sequence.
ACCESSION   DAOSMY010000121 DAOSMY010000000
VERSION     DAOSMY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21961_length_4092_cov_3.229874"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..932)
                     /locus_tag="PLD76_03755"
     CDS             complement(48..932)
                     /locus_tag="PLD76_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOU27150.1"
                     /translation="MKNKNYASDIKTVIDELTGLCPTENMFHPNKEGEPFPSRAIMKD
                     SIKLTRSILFPGFFGNASVNKTTLPYHIGVNVEKLFTMLWEQVYAGLCFDCKEEDIDL
                     EAKREKAFTITLSFVKQLPRIKEVLRTDVEAMYKMDPAAKSYGEIIISYPGLRAITNY
                     RIAHELHKLQTPIIPRIITEMAHAETGIDIHPGARIGESFAIDHGTGVVIGETCIIGN
                     NVRIYQGVTLGAKSFPVDENNIPIKGIDRHPIIKDNVIIYASATILGRIIIGENAVIG
                     GNTWITEDVKPNEKVFVK"
     gene            complement(951..2525)
                     /locus_tag="PLD76_03760"
     CDS             complement(951..2525)
                     /locus_tag="PLD76_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU27151.1"
                     /translation="MKTPILSILLLVGWFATFAQTSAQFEMQKLNFSYGAITNFYVDK
                     VDPSKLTEWAIKGMLDELDPHSSYLSADEVKESTEALQGSFDGIGVSFNMVEDTLVVI
                     QSITGGPSEKLGILPGDKIILVNDTTIAGVKMKTSDITKRLKGPKGTQVKVTILRNNE
                     SIPFTIIRDRIPIFSVDAFYMVDNKIGYIKVNRFASTTHQEVVDGIKNLKKKGMESLI
                     IDLQGNGGGYLQSAIEIVDELLPNKRTVVYTEGANHAKQTMTTRRNGLMESGKVVVLV
                     DEYSASASEIVAGALQDWDRGVVIGRRTFGKGLVQRPIPMPDGSEIRLTVARYYTPSG
                     RSIQRPYEKGKEKYEEDILDRYKHGEFVSADSITFADSLTYKTLVTQRTVYGGGGIMP
                     DIFVPADTTKYTTLHRQLIRNHIITKISIVYAEKNRALVSKQFSTFEAFKLKFDTQEV
                     LSHIKQETLAKEIAFTEEEWNETKDLLAIQAKAIIARNLWSTNEYFEIINETNDFVKK
                     AIEVLKSESIYYNTLQ"
     gene            complement(2631..>4092)
                     /locus_tag="PLD76_03765"
     CDS             complement(2631..>4092)
                     /locus_tag="PLD76_03765"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU27152.1"
                     /translation="INPDIIALQEVGTSTTYATIDTLVKKLGINEWAGAIVATTHTNC
                     GQNQGIIYKKFHLQLVNSSLLSNGANSQGNSYYYNWTNGRYPVIHDFVLTIGSTNIPF
                     SIINTHAKAMSDETSYSRRLGASEGLKGILDGTNYNTSKIIITGDFNDYLIGSQTSSY
                     SNSPYKNFMDDTENYQSLTSSLTDPYYSSPVIDNIIITNELASHYVANSTIRETSATN
                     TISNYRNTTSDHTPISALFRFTSNNPTDCQAIDFSESFASSLGDFIPYSVEGTQTWHW
                     RNIYGAYVSGYASSTNNPNEDWLISPAFDLSDKESGSLSFEHALNYAQEANERANNHT
                     LWISTNYTEGDPKNANWTQLSIPTMPLGTSWTYVNSGSINLPSQWMKENVRFAFKYMA
                     TSSMAGTWEIKNLLFNATCEPVAIPSISNDNKVVFAQNRNITVLLEHETSIRIFNILG
                     NTLYNDTRSENSVFPVSQAGIYLVCIGNECYKIAVR"
BASE COUNT         1096 a          777 c          812 g         1407 t
ORIGIN      
        1 tcaacataaa atgggtcagc ctattacact ttttttaagg gtatggctta ttttacaaat
       61 actttttcgt ttggtttcac atcttcggta atccatgtat ttccgcctat tacagcattt
      121 tcgccaatga tgattctacc caaaatagtg gcactggcat aaataatcac attgtctttg
      181 ataatgggat gtctgtctat gcctttaatt gggatattat tttcatcaac aggaaaactt
      241 ttagcaccca gcgttacccc ttgataaata cgcacattgt ttccaataat acatgtttcg
      301 ccaattacca ctcctgtgcc gtggtcaatg gcaaatgact cgccaatacg tgccccaggg
      361 tgaatatcaa tacctgtttc ggcatgagcc atttcggtaa ttatgcgtgg aatgataggt
      421 gtttgtagtt tgtgtaattc gtgtgcaatt cggtaattgg taatagccct aagtcctgga
      481 tacgaaataa tgatttcacc gtaactttta gcagctggat ccattttata cattgcctct
      541 acgtctgtac gcaacacttc ttttatacgg ggtaattgtt ttacaaacga aagggtaatc
      601 gtaaatgctt tttcgcgttt agcttccaag tcaatgtctt cttctttaca atcaaaacac
      661 aaaccggcat atacttgttc ccaaagcata gtaaataatt tttccacatt tacaccaata
      721 tggtatggca aggttgtttt gttgacactc gcattgccga aaaaacctgg aaacaaaata
      781 gaacgtgtta gtttgatact atctttcatt attgcccgtg acggaaatgg ttcgccctct
      841 ttgttggggt gaaacatatt ttctgttggg cataagcctg ttaactcgtc gataacagtc
      901 ttaatatcgg aggcgtaatt cttgtttttc atcagtttac gttcttattc ttattgcagg
      961 gtgttgtaat atatcgattc gctttttagt acttcgatgg ctttttttac aaaatcgttt
     1021 gtttcgttta ttatttcaaa atattcgttt gtcgaccaaa gattgcgtgc aatgattgct
     1081 tttgcctgta ttgctaacaa gtctttcgtt tcgttccatt cttcttccgt aaacgcgatt
     1141 tcttttgcta aggtttcttg ttttatatgg gagagaactt cttgtgtgtc aaatttcaat
     1201 ttaaacgctt caaaagtact aaattgtttt gatacaagag ctctattttt ttcagcatat
     1261 acaatactta tttttgtaat gatatgattg cgtatcagtt gacggtgcaa ggtggtatat
     1321 ttagttgtgt cagcaggcac aaaaatatca ggcataatgc cacctccgcc atatacagta
     1381 cgttgggtaa caagggtttt gtatgtaagt gaatcggcaa atgtaatgct gtcagccgat
     1441 acaaactccc catgtttata gcgatcaaga atatcttcct cgtatttttc ttttcctttt
     1501 tcgtaaggac gttgaatgct gcgtccagaa ggggtgtagt aacgagctac ggtaagccta
     1561 atttccgaac catcgggcat aggtattgga cgttgtacca atcctttgcc aaacgtgcgt
     1621 ctgccaatca ctactcctct gtcccaatct tgcaaagctc ctgctacaat ttcgctagcc
     1681 gaagcagaat attcatctac taatacgact actttgccac tttccataag tccattacgg
     1741 cgagttgtca ttgtttgttt ggcatgattg gctccttcag tatacacaac tgtacgttta
     1801 tttgggagca gttcatctac tatttcaatc gcactttgca aatagccacc tccatttcct
     1861 tgtaaatcaa tgataagcga ctccatgcct ttctttttca aattttttat gccatctacc
     1921 acttcttgat gggtggtgct ggcaaaacgg ttgactttga tatatccaat tttattgtct
     1981 accatataaa aagcatctac actaaaaatt ggaatacggt cgcgaatgat agtaaacgga
     2041 attgattcgt tgtttcgcaa aatagttact tttacttgtg ttcctttggg accttttaag
     2101 cgtttggtaa tgtcgctagt tttcattttt acgccagcaa tcgtggtatc gttaaccaag
     2161 attattttat cgccaggcaa tatgcctagt ttttcggatg gtccgccagt tattgattgg
     2221 attactacta aggtgtcttc taccatatta aacgatacgc caatgccatc aaaactacct
     2281 tgaagtgctt ctgtcgattc ttttacctca tctgctgata gataactaga atgagggtct
     2341 agctcatcaa gcatgccttt gatagcccat tcggttagtt tactagggtc aactttatct
     2401 acataaaaat tggtaatagc tccatacgaa aagttgagtt tttgcatttc gaattgggct
     2461 gatgtttgtg caaaagtggc aaaccaacca acaagaagga gtatagaaag gataggagtt
     2521 ttcatacagt attttttttc gtaacaacaa aggtacgcaa aacattttat ttaacagaaa
     2581 tagaaaagta gagaaatggt taaaaaagta gaactagtac tatttgttct ttatcttacc
     2641 gcaattttgt aacattcgtt tcctatacac acaaggtaga tacctgcttg cgaaactgga
     2701 aatacgctgt tttcgctgcg ggtgtcgtta tacaatgtgt ttccaagaat gttgaatatt
     2761 ctgatggatg tttcgtgttc caacaaaaca gtaatgttgc gattttgtgc aaatactacc
     2821 ttgttatcgt ttgatataga gggtattgct actggttcgc aagtagcatt gaatagcaga
     2881 ttttttattt cccacgtacc agccatgctt gatgttgcca tatatttaaa cgcaaaacgc
     2941 acgttttctt tcatccattg gctaggcagg tttatgctac cagagtttac gtatgtccag
     3001 ctagtgccca atggcattgt aggaatactt agttgtgtcc aattggcatt ttttgggtct
     3061 ccttcggtgt agtttgttga aatccataag gtgtgattgt tggctcgctc gtttgcttct
     3121 tgagcatagt taagggcatg ttcaaacgac aaggagcccg attctttgtc ggataaatca
     3181 aaagcaggcg aaataagcca atcttcgttc ggattattcg tgctactggc atatcctgac
     3241 acgtatgctc cgtaaatatt tcgccaatgc catgtttgtg tgccttctac agaatagggg
     3301 ataaaatcac ccaagctact tgcaaagctc tcagaaaaat ctatcgcttg gcagtcagtt
     3361 ggattgttgc ttgtgaaacg gaacagtgca cttattggcg tatgatcgga ggttgtgttg
     3421 cggtaattgc ttatcgtatt ggtggcacta gtctcgcgta tggtgctatt cgcaacataa
     3481 tggctagcca gttcatttgt aataatgata ttgtcaatca cagggctact gtaataagga
     3541 tcggttaagg aagaggtaag actttggtaa ttttccgtat cgtccataaa gtttttgtac
     3601 ggagaattgg agtacgaaga agtttgcgaa cctattaaat agtcgttgaa atcgcctgta
     3661 attataattt tgcttgtatt gtagttcgta ccatctagta tcccttttag cccttcggaa
     3721 gctccaagcc ttcggctata gctggtttca tcactcatgg ctttggcgtg tgtattgatg
     3781 atggaaaacg gaatatttgt tgagccaata gtcaatacga aatcatgtat aacaggataa
     3841 cgaccattag tccaattata gtagtaactg tttccttggc tatttgcccc gttactcagt
     3901 agggaagaat taacaagttg taggtggaat tttttgtaaa taatgccttg attttgaccg
     3961 caattagtgt gagtagtagc cacgatagct cctgcccatt cgtttattcc aagttttttc
     4021 actaaggtat cgatggtggc atacgtagtg cttgtaccta cttcttgcaa ggcgataata
     4081 tcaggattta tc
//
LOCUS       DAOSMY010000122         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_22099_length_4073_cov_2.864360, whole genome shotgun sequence.
ACCESSION   DAOSMY010000122 DAOSMY010000000
VERSION     DAOSMY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22099_length_4073_cov_2.864360"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLD76_03770"
     CDS             <1..335
                     /locus_tag="PLD76_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU27153.1"
                     /translation="YGSVQTTLLEKNLSWIGLFIGLAPMLGFLGTVIGMIEAFDRIQQ
                     AGDISATIVAGGIKVALITTVGGLIVAIILQVFYNFIVSRIESVVSDMEDSSISMLDI
                     LVKQSNKK"
     gene            364..816
                     /locus_tag="PLD76_03775"
     CDS             364..816
                     /locus_tag="PLD76_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27154.1"
                     /translation="MLNNLSKIILYVLIGITVVVSVLFFWGGAVDEYAEYIEPNYTQA
                     LMALMYVFFGIAIVITIVSLLISFLQNFKVDKKTATKPIISIVALLALIGFAWLIADG
                     SSMQILGMDVQPTENEIKFVDVQIKAIYLLSGVAVLSMFLSFFTKRLK"
     gene            854..1441
                     /locus_tag="PLD76_03780"
     CDS             854..1441
                     /locus_tag="PLD76_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU27155.1"
                     /translation="MSRNKRKVPEINSSSMADISFLLLIFFLVTTMVVNKGLARRLPP
                     PIPPDQKTEDLKVKERNVLVVLLNSDNQLMVQREYISVTELRKKAKEFIVNANNAQDM
                     PEKKAEEVQFFGEIPLTRDHVISLQNDRTTSYKAYIEVQNELIAAYNELREELAKSKF
                     GVSYAELDDDRKTAIQYIYPQKISEAEPKNYGGNK"
     gene            1441..1923
                     /locus_tag="PLD76_03785"
     CDS             1441..1923
                     /locus_tag="PLD76_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU27156.1"
                     /translation="MGKFNKNSSSRELPTISTASLPDIIFMLLFFFMATTQLKEVDFK
                     VSIKEPKATELEKLDKKNLVRFIYVGTPTDKYRGVYGSETRVQLDDAFADVSQIEDYI
                     VAQRAAMNEYEQSLLTVALRVDEDAKMGIVTDIKQALRRAYALKINYVSRQRDNSQAF
                     "
     gene            complement(1989..2513)
                     /locus_tag="PLD76_03790"
     CDS             complement(1989..2513)
                     /locus_tag="PLD76_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU27157.1"
                     /translation="MQQTEWFPIVTETGERIGKATRKECHSGSFLLHPVVHLHVFNAK
                     KQLFLQQRAANKDIQPNKWDTAVGGHVDDGESIQIALVREAYEELKIKDFSANFLFQY
                     PFRSQVEHELVHSFWVQYDFPIQANLEEIQDARFWEIDEIKNSLGKQLFTPNFEDELT
                     KLLSHPDIIQFLHP"
     gene            2614..3966
                     /locus_tag="PLD76_03795"
     CDS             2614..3966
                     /locus_tag="PLD76_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU27158.1"
                     /translation="MNYHRIFRTYFLYKLIFKYVRCIHAYILHRSYTVYNAENIPAGK
                     PFIMVANHQHGILDALALLFMIKDGRYPVFMARADVFKNKYVAQLLVFFRLLPAFRMQ
                     DVGKNRVLENEKVFATTAQLLAQHNGILALFPEGGHQEREQLGKLKKGFARIAFQTAS
                     CANFETEIFVLPVGIHHIGGLFQSKVSVCVGKPISVLTFQELYMQEPEKAQQQLLEQT
                     ARAMSSNMLDIKETTYYNEYLVLCTIARIYRKNNAAIQERYATDLAIEQAVVSRVELL
                     KVHQETTYVELMKLTKNMDELIVSLHLSYELVCEKKHSIAKRGMHVFITILLIPIQIY
                     AFVHQIIPFLLTSVCSRNAGDELLKVSLQLALGALFIFPLWNGFLVFILFLLEVPFSW
                     LVCYGISLLFSIWVFFRSVSAWSFCQAWCRRQLVRLTNRSAIKEIEGILKNIFSIIGV
                     "
BASE COUNT         1293 a          584 c          809 g         1387 t
ORIGIN      
        1 cttatggaag tgtacaaaca actttactag aaaagaattt atcttggata ggtttgttta
       61 ttggattagc tcctatgtta ggattcttgg gaacagtaat tggtatgatt gaagcttttg
      121 accgtattca acaagcaggt gatatttctg caactatcgt agcaggaggt atcaaggtag
      181 ctttgattac tactgttgga ggtcttattg tagctattat tttacaagta ttttataact
      241 ttattgtttc gcgtattgaa tcagtagtaa gcgatatgga agattcatca atctctatgt
      301 tggatatatt ggtaaaacaa agcaataaga aatagattat tttacaactt aaaataaaaa
      361 aacatgttaa acaatctttc aaaaataatc ctctatgtcc tcataggaat tacagtagta
      421 gtctctgtct tattcttttg gggaggtgca gtagatgagt atgctgaata tatagagccc
      481 aattacacac aagctttaat ggcgttgatg tatgtgtttt tcggaatagc tattgttatc
      541 actatagtgt cgctacttat ttcgtttttg caaaatttta aagtagataa aaaaacggca
      601 acgaagccta tcattagtat tgttgctttg cttgctttaa taggttttgc ttggttaata
      661 gcagatggta gttctatgca aattttgggt atggatgttc agcctactga aaatgaaatt
      721 aagtttgtag atgtgcaaat aaaagcaatc tatctattgt cgggtgtagc agtgttgtca
      781 atgtttttaa gcttctttac aaaaagatta aaataagttg taatacacga atcatattaa
      841 attaataaat agaatgagta gaaataaaag aaaagtacct gaaattaatt caagttcgat
      901 ggcggatatt tccttcctgc tcttgatttt ctttttggta actactatgg tggtaaataa
      961 agggcttgct agaagattgc ctccacccat tccgcctgac caaaaaacag aagatttaaa
     1021 ggtaaaagag cgtaatgtat tggtagtgtt gctgaattcg gataatcaat tgatggtgca
     1081 acgtgaatat atcagtgtaa ctgaattgcg taagaaagca aaagaattta tcgtaaatgc
     1141 gaataatgct caagatatgc ctgagaaaaa agctgaagaa gttcaatttt ttggagaaat
     1201 tcctcttaca cgggatcatg tgatttctct tcaaaatgat agaactactt cgtacaaagc
     1261 atatattgaa gtgcaaaatg agcttatagc tgcatataat gaattgcgtg aagaattggc
     1321 aaaatcgaag tttggagtat cgtatgcgga gctagatgat gatagaaaaa cagccataca
     1381 atacatttat ccacaaaaaa tttcggaagc ggaaccaaaa aattatggag ggaataagta
     1441 atgggaaaat ttaataaaaa tagtagttcg agagaattgc ctactattag taccgcatca
     1501 ttgcccgaca ttatttttat gttgttgttc ttctttatgg caaccacaca attaaaagag
     1561 gttgacttta aggtgtccat taaggaacct aaggctactg agttggaaaa attggacaag
     1621 aagaatctgg ttcgctttat ttatgtaggt actccgactg ataaatacag aggtgtatat
     1681 gggtctgaaa cgcgtgtaca acttgatgat gcttttgctg atgtatcgca aatcgaagat
     1741 tatattgtag ctcaacgtgc ggctatgaat gagtatgagc aatcgctact aactgtagcg
     1801 cttagggttg acgaagatgc taagatgggc attgttacag atattaaaca agctttgcgt
     1861 agagcatatg ccttaaagat taattacgtt tctaggcaac gcgataatag ccaagctttt
     1921 taaaatcaac agtgatggtt gaaataaata aaaaagagaa gtcttagata ggaggcttct
     1981 cttttttttc agggatgtaa aaattggatg atatcagggt gcgatagtag cttggttaac
     2041 tcatcttcaa agtttggagt aaacaattgt ttccccaaag aatttttaat ctcatctatc
     2101 tcccaaaacc tagcatcttg aatttcttct aagttggctt gaatgggaaa atcgtattga
     2161 acccaaaagc tatgtactaa ttcgtgttct acttgcgagc gaaagggata ctggaataga
     2221 aagttcgccg aaaagtcttt tatttttaat tcttcatacg cttcgcgaac taaggctatc
     2281 tgtatgcttt cgccatcatc tacatgccca cccacggcag tgtcccattt atttggctga
     2341 atgtctttat tggctgcacg ttgttgcaaa aataattgtt tcttggcgtt aaatacatga
     2401 aggtgtacca ctggatgaag gagaaaagac cctgaatgac actcttttcg ggttgcttta
     2461 ccaattcttt cgccagtttc ggttactata ggaaaccatt ctgtttgttg cataacatta
     2521 tttttgtaca aaagtagaaa atatttctta ttttaagcgt atacgagttg tctgaatact
     2581 gtatctttgt ttttttatta gaacaattaa cgaatgaatt atcatcgtat ctttcgtact
     2641 tattttttat acaaactgat ttttaaatac gttcggtgta tacatgctta tatattgcat
     2701 cgttcatata ctgtatataa tgcagaaaat atccctgctg gcaagccatt tattatggta
     2761 gccaatcatc aacatggaat tttggatgct ctggcattat tgtttatgat taaagatggt
     2821 agatatccgg tgtttatggc acgtgctgat gtgtttaaga ataagtatgt tgcacaattg
     2881 ttagtttttt ttcgtttact accagccttt cgtatgcaag atgtaggtaa aaatagggtg
     2941 cttgaaaatg aaaaagtgtt tgcaactacg gctcagttac ttgctcagca taatggcatt
     3001 ttagcacttt ttcctgaagg ggggcatcaa gaaagggaac aattaggaaa actgaaaaaa
     3061 ggatttgctc ggatagcctt tcaaacagcc tcttgtgcca attttgaaac agaaattttt
     3121 gtattacccg tgggtattca tcatatagga ggattgtttc agagtaaggt ttcggtgtgt
     3181 gttggcaaac ccatttctgt tcttactttt caagaactct atatgcaaga gccagaaaaa
     3241 gcacaacagc agttgcttga acaaacagca agagctatga gtagtaatat gctagacata
     3301 aaagaaacaa catattacaa cgaatatttg gtgctgtgta cgatagctcg tatatatcga
     3361 aaaaataatg cagctattca agagcgttat gctactgatt tggctataga acaagctgtt
     3421 gtaagtaggg tagaattgct taaagtacat caagaaacaa catacgtaga gttgatgaag
     3481 ttgactaaaa acatggatga actaattgta agtttgcatt tgtcatacga attggtgtgt
     3541 gaaaaaaaac actctattgc gaagcgaggc atgcatgttt ttattacgat actattgatt
     3601 cccatacaga tatatgcatt tgttcatcag attatccctt ttttattaac tagtgtatgt
     3661 agtagaaatg cgggagatga gttgctaaaa gtttctttgc aattagctct aggagcttta
     3721 tttatttttc ctttatggaa tggattttta gtgtttattc tatttttact agaagttcca
     3781 ttttcttggt tggtttgtta tggcatatct cttcttttta gtatttgggt gttttttagg
     3841 agtgtttctg cttggagttt ttgtcaagct tggtgtcgca gacaactagt aaggctgact
     3901 aaccgttctg caataaaaga aatagaggga atattaaaaa acattttctc aattataggg
     3961 gtataattag tttgtttata taaagatata ttctttttgt ataaaatatt ttatttttca
     4021 gataagaaat tttaccttta gacgtttttt agataattta ttcctatgat acg
//
LOCUS       DAOSMY010000123         4063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_22173_length_4063_cov_3.584331, whole genome shotgun sequence.
ACCESSION   DAOSMY010000123 DAOSMY010000000
VERSION     DAOSMY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22173_length_4063_cov_3.584331"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PLD76_03800"
     CDS             <1..169
                     /locus_tag="PLD76_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU27159.1"
                     /translation="FFHVAINIGMVIGLVPVIGIPLVFLSYGGSSLWSFTILLFTYIR
                     LDANRMEILHQ"
     gene            347..592
                     /locus_tag="PLD76_03805"
     CDS             347..592
                     /locus_tag="PLD76_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU27160.1"
                     /translation="MNIYIGNLNYRVRENDLKGIFEEYGTLDSVKLVKDRETGKSKGF
                     AFAEMSNDSEAKKAIEELNGAEFEGRTLVVKEANPRN"
     gene            complement(650..1621)
                     /locus_tag="PLD76_03810"
     CDS             complement(650..1621)
                     /locus_tag="PLD76_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU27161.1"
                     /translation="MNKIDEIKQPIIADLLAYEKELALALQTENPILNQLSSYVLSTR
                     GKQMRPILSLLVAQVCGGITESTIQGTIAIELLHIASLIHDDVLDSALERRNKPSVNA
                     VFENKIAVLGGDYLLSKALSKASNSRNLDIIFEISKVGSNLVDGEILQLANSKKQFIT
                     EDIYFDIISKKTASLFSSCARIGALSANATNHEIDCLGIYGTSIGICFQLRDDIFDYF
                     SDDVGKPTGNDLREGKLTLPMLFALSKATNSEKEYLFSLLRKADLTEKEVQELQQFSI
                     QKGGVEYATKKMYEYRDKAIRQLQHFATSATKNALMEYATYVIERNK"
     gene            complement(1626..2582)
                     /locus_tag="PLD76_03815"
     CDS             complement(1626..2582)
                     /locus_tag="PLD76_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOU27162.1"
                     /translation="MMYAERSKEVFRQEIQALEKVASRIGAEMDKVVELLFASTGKIV
                     VTGIGKTGIIAHKIASSLASTGTPAFFMNAAEAMHGDLGMISANDVVLIVSNSGASQE
                     ILRIIPPIKHINCPIIALTGNVSSPLANEADYVLDVSVDKEACPLGLAPTTSTTATLV
                     MGDALIVSLMEKRHFKADDFALNHPGGALGKRLLVKVKEIMQTTIPYVYEKTPFKDVI
                     YEISNKRLGMTIVMNTNNTPIGIITDGDVRRAIQKYQDISNLTAYDFMTVGYKSITEN
                     ERIDDALRIMETNKITTLAVVDAATPTIIGIIHIHDIFAYRK"
     gene            complement(2602..3438)
                     /gene="kdsA"
                     /locus_tag="PLD76_03820"
     CDS             complement(2602..3438)
                     /gene="kdsA"
                     /locus_tag="PLD76_03820"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOU27163.1"
                     /translation="MIIPSTQQVVIPNHFVIGGTSRFVFIGGPCAIESEQMSIDVAGS
                     LKEICDKLGIHFVFKSSFDKANRSSVFSPRGVGMDKGLSILQKVTDTYGIPILTDVHE
                     SWQCDAVGSVVDVIQIPAFLARQTDLLVAAAKTGKVVNIKKAQFMAPWDMQQVVNKMI
                     ETGNDTIVLTERGSSFGYNNLVVDMTSLIEMRKIGYPIVFDATHAVQKPGGNGINSGG
                     NRSYVAPLMRAALAVGVDAIFAEVHPNPDMAFSDAANQLHLDSIHTILEQAVTIDDII
                     KK"
     gene            complement(3435..3980)
                     /locus_tag="PLD76_03825"
     CDS             complement(3435..3980)
                     /locus_tag="PLD76_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU27164.1"
                     /translation="MNVCVFCSSSNEIAEVYREDARLLGEVIGKKSYTLVYGGANMGL
                     MQECAMAVKLSGGSVIGVIPDSFRKKVEQKVLDQVFFVGSLAERKELMMEYADVFVAL
                     PGGIGTLDEVFEVLALAMIDNHHKKVLFLNTHGYYTHLVAHIEHSIQEKCVHPAMKNS
                     YIVVNTVDECMAYIDKENVVK"
BASE COUNT         1259 a          812 c          618 g         1374 t
ORIGIN      
        1 tttctttcat gttgctataa atatcggaat ggttattggt ttagttcccg ttattggtat
       61 tccgttagtt tttttaagct atggaggttc ttctttgtgg agcttcacaa tattattgtt
      121 cacatatata cgtcttgatg ctaatagaat ggaaatatta catcaataac tcctgtgagg
      181 tatataaaat tataaccgaa acgtaaccta aataatgaaa tatacaacaa aatccacatt
      241 ttttttcaaa ttatggtatt ttattaaaat attatgcttt cctttgtgtc gtaaatcatt
      301 aagtgaagat ttacgacata tttatttatt tttttaattt ttttttatga acatttacat
      361 tggaaacttg aattatcgag ttagagaaaa cgacctaaaa ggtatctttg aagaatacgg
      421 aacattggat tctgtaaaac ttgtaaaaga cagagaaaca ggaaaatcga aaggttttgc
      481 atttgcagaa atgtcaaacg acagcgaagc aaaaaaagct atcgaagaac tgaatggtgc
      541 tgaatttgaa ggaagaacat tagtggttaa agaagcaaat cctagaaatt aattgatatt
      601 atttttatgg gaattaaaaa gaggctattc aaatgaatag cctctcttgt tatttgtttc
      661 gttctataac ataggttgca tattccatca aggcattttt agttgcagaa gtagcaaaat
      721 gttgtaattg acgaattgct ttatctctgt attcatacat tttcttggtt gcatattcca
      781 ctcccccttt ttgaatggaa aattgttgca attcttgcac ttctttttca gtcaaatctg
      841 ctttccgaag caaagaaaat agatattctt tctcgctatt cgttgcttta ctcagagcaa
      901 acagcatagg taaagtaagc tttccttctc gaagatcatt tcctgtaggc ttgcctacat
      961 catccgaaaa ataatcaaat atatcgtctc ttaattgaaa acatatccca atgctcgtac
     1021 cataaatacc taaacaatca atttcgtgat tggtagcatt tgctgatagt gctcctatgc
     1081 gggcacaact agaaaaaaga ctagctgtct tttttgaaat aatatcaaaa tagatatcct
     1141 cggttataaa ttgcttttta gaatttgcca gctgcaaaat ctctccatcc accagatttg
     1201 atcctacttt agatatttca aagataatgt ctaagtttct actgtttgaa gccttggata
     1261 aggctttaga taacaaataa tctccaccca atacggcaat cttgttttca aacaccgcgt
     1321 ttactgatgg tttgtttctt cgctctaagg cactatcaag tacatcatca tgaattaaac
     1381 tagctatatg tagcaactca atagcaatag taccttgtat agttgattca gtaatgccgc
     1441 cacagacctg agcaactaac aacgacaaaa taggtcgcat ttgtttaccc ctcgtgctta
     1501 atacgtagct tgacaattga ttgagaattg gattttctgt ttgaagagct aaggctagtt
     1561 ctttttcata tgccaacaaa tctgcaatta ttggttgttt aatttcgtct attttgttca
     1621 taaacttatt ttctgtatgc gaaaatatca tgtatatgta taatgccaat aatggttggc
     1681 gtagcagcat ctactacagc caatgtggtt attttatttg tctccattat tcttagtgca
     1741 tcatcaatac gttcattttc tgtaattgat ttatagccaa cagtcataaa atcatatgct
     1801 gttaaattag agatatcctg atatttctga atagcccttc tcacatctcc atctgtaata
     1861 atgccaatag gtgtattgtt cgtattcatc acaattgtca tacccaatcg tttattacta
     1921 atctcatata taacatcttt gaaaggtgtt ttttcgtaca catagggtat ggtggtttgc
     1981 ataatctctt ttacttttac tagtaatcgt ttccccaatg cacctccagg atgattaagt
     2041 gcaaaatcat cggctttaaa gtgtcgtttt tccatcaaac taacaattaa agcatctccc
     2101 attactaatg tggctgtggt agaagtagtt ggagctaacc ccaacggaca agcttcctta
     2161 tcaacactca catcgagaac ataatcagcc tcgttagcaa gtggagaaga aacattccct
     2221 gttaatgcta taatcggaca attgatatgc ttaataggag gtataattct caatatttct
     2281 tgcgaagctc cactgttaga tacaatcaac actacatcat tggcggatat catacctaaa
     2341 tctccatgca ttgcttctgc cgcattcata aaaaaagcag gagttcctgt agaagccaac
     2401 gaagaggcaa ttttatgtgc aataataccc gttttaccaa tacccgttac cacaatcttt
     2461 cctgtactag caaacaataa ctcaactact ttatccattt ctgcacctat gcgtgatgct
     2521 accttttcta aagcttgtat ctcctgccta aaaacttctt tgctacgttc agcgtacatc
     2581 atacgatcgg gtattagtct actatttttt tataatatca tctattgtta ccgcctgctc
     2641 aagtattgta tggatactat ccaagtgcaa ttggttggcc gcatccgaaa aagccatatc
     2701 tggattagga tgtacttcag caaaaatcgc atcaacacct actgccaatg ctgctcgcat
     2761 taatggagca acataggagc gattgcctcc tgaatttatt ccatttccac caggtttttg
     2821 cacagcgtgc gtagcatcaa aaacgatagg ataacctatt ttacgcattt ctattaatga
     2881 tgtcatatct actaccaaat tattataccc aaaactggat cctctttctg ttagtacaat
     2941 agtatcattt cccgtttcta tcattttatt taccacttgt tgcatatccc acggagccat
     3001 aaattgggct ttttttatat tcaccacttt gcccgtttta gctgctgcaa ctaataaatc
     3061 ggtttgccta gctaaaaaag caggtatttg tataacatct actacggaac ccactgcgtc
     3121 acattgccaa ctttcgtgta catctgtaag gataggtata ccatatgtat ctgtaacctt
     3181 ttgtaaaatt gataaccctt tgtccattcc tacaccacgg ggagaaaata cagaagagcg
     3241 atttgcttta tcaaaagatg atttaaaaac aaaatgtata cctaatttat cacaaatctc
     3301 tttcaaactg cctgccacat ctattgacat ttgttcgctt tctatggcac agggtccacc
     3361 aataaaaaca aaacgactag ttcctccaat aacaaaatga ttggggatta ctacttgctg
     3421 tgtacttgga ataatcattt tacaacattt tctttatcaa tgtatgccat acattcgtct
     3481 actgtattaa ctacaatata cgagtttttc atcgcaggat gcacacattt ctcttgtata
     3541 ctgtgttcta tatgagcaac taaatgtgta tagtagccat gggtatttaa aaacaatact
     3601 ttcttgtgat ggttatctat cattgctaag gcaagaactt caaatacctc atctaatgta
     3661 ccaatccccc caggcaaagc cacaaagaca tctgcatatt ccatcattag ttcttttctt
     3721 tcagccaaac taccaacaaa aaagacttga tcaagaactt tttgctctac tttttttcta
     3781 aaagaatctg gaataacgcc aatcacacta cctcctgata gttttaccgc catcgcacat
     3841 tcttgcatca aacccatatt ggctcctcca tataccagag tatatgattt tttaccaatt
     3901 acttctccta gcaaacgagc atcttcacga tacacttcgg ctatttcatt gcttgaagaa
     3961 caaaagacac acacattcat cacagttcca ttttttacgc atcaatgcgt gcataggtag
     4021 catttttttc tataaaatca cgtcttggag caacatcatc acc
//
LOCUS       DAOSMY010000124         3983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_22795_length_3983_cov_3.325356, whole genome shotgun sequence.
ACCESSION   DAOSMY010000124 DAOSMY010000000
VERSION     DAOSMY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22795_length_3983_cov_3.325356"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLD76_03830"
     CDS             <1..327
                     /locus_tag="PLD76_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27165.1"
                     /translation="DYCMKVVPGTTVPHDFSSTSLAEYYYMLGEKEKATDLINTIGNR
                     SIAYLEWISKLSPQKKASAAYDAHIQLSSLRNIAYITQNYDEKTFEKYYASLMQYASI
                     FDHLLR"
     gene            336..980
                     /locus_tag="PLD76_03835"
     CDS             336..980
                     /locus_tag="PLD76_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU27166.1"
                     /translation="MLIEQVPQCIRRFFQPVIWRGNAKEKKIFLTFDDGPTPEITPWI
                     LDLLDTYNVKATFFCIGNNVEKYPDLFLEIQQRGHQVGNHAYTHKRGMFTDTKIFFED
                     IARAQKLINSDLFRPPHGHITPRQIKKLKHTFKIVLWDVITRDYDASLPPEIILQNVK
                     KYSRNGSIIVFHDSLKAEKNLRYALPKSLAFWKEKGYECSLIHPTITTSSDKNR"
     gene            1661..3868
                     /locus_tag="PLD76_03840"
     CDS             1661..3868
                     /locus_tag="PLD76_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU27167.1"
                     /translation="MVGDVTDAHSGDHLPYVNVVIKGTTIGIMTDASGHYFLKNLPIG
                     TFTIIYSMVGYQTVERTITIEPNKTLELKIKLEETSIMMDNIVVTGNKYETKKKETSS
                     IVNVVTPLIFTSTSANSIGEALDYQTGLRTEMTCGNCGVPQLRINGLEGHYSQLLLDS
                     RPLFSSLASVYGLEQMPTGMIDRVEVIRGGASALYGSNAIGGVVNIITKEPTRNFVEL
                     SNNTALMGNTAIDANTTFNASIIGNNSNVGVFVFGVLRDRQAYDRDNDGFSEMPALQG
                     SVVGFRSYFKTSNYSKITTEYHHSTEYRRGGDLLDLPAHEANLAEQLQHNIDAGSVKF
                     DLYTKDNKHFISTYSAFQHIVRNSYFGTGQDIDAYGKSKDINSVTGAQYRYSMDNLLF
                     MPADFSMGIEYTYNQLQDQMLGHGIDVNQNVSIFGGYLQNEWKNNHLSLLAGARVEKH
                     NLMNNPVVSPRVSLRYNPTHNIVLRTGYASGYRAPQIYNEDLHVAAVGGEVSIISQDA
                     NLRPEYSHSFSVSADAYHRFGFWETNLTVDGFYTKLNDVFALEENGFDSKGNLLLLRV
                     NESGATVTGVNIEGVLGYKNLLSLQLGYTLQSSLYDEPVTWSTNTAIEATRRMQRTPD
                     HYGYAVMNYNATKNFTASVSGKYTGSMLVPHFAGYIAEDTETISSPFWDMGVKLAYKI
                     PFYKYYALEVNGGVKNILDSFQPDLDKGMDKDAGYIYGPTLPRTFFVGLSLKL"
BASE COUNT         1383 a          733 c          676 g         1191 t
ORIGIN      
        1 gactattgta tgaaagtagt tccaggtact acagtgcctc acgacttttc ttctacctca
       61 ttagctgaat attattatat gcttggagaa aaagagaaag caactgattt aattaatact
      121 attggtaatc gatccattgc ctatttagag tggataagta aattgagtcc acagaaaaaa
      181 gcttctgcag catatgatgc tcatattcaa ctatcttcgc tcagaaatat tgcttatatc
      241 acacaaaatt acgacgaaaa aacgtttgaa aaatactatg caagcttaat gcaatatgca
      301 agtatatttg atcatttatt gaggtaatag tcattatgct aatagaacaa gttcctcaat
      361 gtataaggcg tttcttccaa ccagtaatat ggagaggaaa cgccaaagaa aaaaagattt
      421 ttcttacatt tgatgatgga cctactccgg aaataacacc ttggatttta gatttgcttg
      481 atacgtataa tgtaaaagct acattttttt gtattggcaa taatgttgaa aaatatcccg
      541 acttgtttct agaaattcaa caacgagggc atcaagtagg caatcatgca tacacccaca
      601 aacgagggat gtttaccgac acaaaaatat tctttgaaga tattgcacgt gctcaaaaac
      661 ttatcaatag tgatttattt agaccaccac atggacacat aacccctcga cagataaaaa
      721 aactcaagca cacatttaaa attgttcttt gggatgttat tacacgcgat tacgatgctt
      781 cgttgcctcc agaaataata ttgcaaaatg taaaaaaata ttctagaaac ggttctataa
      841 ttgtatttca cgactcattg aaggctgaaa aaaacttacg ctatgcacta ccaaaatcat
      901 tagcattttg gaaagaaaaa gggtatgaat gctctctcat tcatcctaca atcactacaa
      961 gtagtgataa aaatagataa atatcactac atatagtttt tttatggtac aatactccca
     1021 ttcttccgtt gtatttttgc accataaaaa tatgaaacgc aagcactcaa cctatataca
     1081 cggattccta gcattcctat ttattgctta ctgggtaagt tctaccttgc tttcgcatac
     1141 gcatattgtt aataatacca ttattacaca ctcgcatcca ttctcttcca agtcccactc
     1201 gcatactcaa gctgattgga tatggaatat tcacaaccaa atagggcaaa taattgtgct
     1261 ctccattagt tctctcgtat gttttagtat acttacgttt ctactacttt acagaaatta
     1321 ctcaatacac atacattctc ataagttaga acataggcac cgaagacctc ctcctctttt
     1381 tttataatga tattgaccta gatatcccaa acacataatt acaatatcaa tgtgtcaatg
     1441 aaacggctag atcaaataca taccgtacaa cctaatttaa gctcaacaca caaacagtaa
     1501 acagtgaata cctgttgttt tataatcaaa acaggtaaca agattttttg tataaatatt
     1561 catagatatg aaaaaaataa ttctatttag cttttttatc ttttcttttt gtgttatttt
     1621 atcagcataa attccagcat ccaaagtaac cgatgcacat attgtagggg acgttacaga
     1681 tgctcactct ggcgatcatt taccctatgt taacgtagta attaaaggaa ctaccattgg
     1741 tataatgact gacgcttcgg gtcattattt tctaaaaaat ctacctatcg gaacctttac
     1801 catcatatat tccatggttg gataccaaac cgttgaaaga acaataacaa tagaacccaa
     1861 caaaacactt gagttaaaaa ttaaactgga agaaacatcc attatgatgg ataatattgt
     1921 agtaacaggg aataaatatg aaacaaagaa aaaagaaaca tcgtctattg taaatgtcgt
     1981 taccccactt attttcacaa gtacttcggc aaacagtata ggcgaagctt tagattatca
     2041 aactgggtta cgtactgaaa tgacatgtgg taattgtggc gttccgcaat tgcgtattaa
     2101 tggattagaa ggtcattatt cacaattatt attagacagt cgcccattat ttagctcgtt
     2161 ggcttcggta tacggactag aacaaatgcc aactggaatg atagatagag tagaagttat
     2221 tagaggtgga gcttctgcat tatatgggtc aaatgcaatc ggaggtgtag taaatatcat
     2281 taccaaagaa cctacacgca attttgttga gctatccaat aatactgcac tcatgggtaa
     2341 tactgcaata gatgccaata ctacgttcaa tgcctcaatt atcggtaaca attccaacgt
     2401 aggcgtattt gtatttggag tattacgcga cagacaggca tacgatagag acaatgatgg
     2461 gttttctgaa atgcccgcat tgcaaggttc tgtagtaggc tttcgctcgt actttaaaac
     2521 tagtaattat tcaaaaatta ctaccgaata ccaccattca actgaataca gaagaggagg
     2581 cgacttgctc gatttaccag cccatgaagc caatttagca gagcaacttc aacataacat
     2641 agatgcaggt agcgttaaat ttgatttgta taccaaagat aacaaacact ttatttctac
     2701 ctatagtgca tttcaacata ttgtgcgtaa tagttatttt ggaacaggtc aagacattga
     2761 cgcctatgga aaatcaaagg atattaattc tgtaacagga gcccaatacc gctattcgat
     2821 ggataatcta ttgtttatgc cagcagattt tagtatggga attgaatata catataatca
     2881 acttcaagac caaatgttag ggcacggaat tgatgtaaac caaaatgtta gcatctttgg
     2941 tggatacctt caaaatgagt ggaagaataa tcacctaagc ctattggcag gtgctcgtgt
     3001 agaaaaacac aatttaatga acaatccagt agtatcgccc cgtgtaagtt tacgctacaa
     3061 cccaacacac aacattgtac taagaactgg atacgctagc ggataccgtg ctccacaaat
     3121 atacaacgaa gatttacacg tagcggctgt gggtggagag gtgtctatta tctcgcaaga
     3181 cgcaaacctt cgtcctgaat attcgcatag tttctctgta tccgcagatg cataccatcg
     3241 ttttggtttt tgggaaacca acttaacagt agatggcttc tatacaaaac tgaatgatgt
     3301 atttgcacta gaagaaaatg gttttgatag caaaggaaac ttattattgt tgcgggttaa
     3361 tgaatcggga gcaaccgtaa cgggagtaaa tatcgaagga gtgttaggat ataaaaactt
     3421 actatcacta caattaggct ataccttaca aagtagctta tatgatgaac cagtaacctg
     3481 gtcaaccaat actgctatcg aagccacaag acgcatgcaa cgcacaccag accattatgg
     3541 ttacgctgtt atgaattata acgcaactaa aaactttaca gcatcagtat ctggtaaata
     3601 tacaggaagt atgctagtac cacattttgc aggttacata gccgaagata ctgaaacaat
     3661 ttcatctcca ttttgggata tgggggttaa actagcatac aaaatacctt tttacaaata
     3721 ctatgcactt gaagtaaacg gaggtgtaaa aaatatactg gatagctttc agcctgactt
     3781 agataaaggt atggataaag atgctggata tatttacgga cctacattgc ctcgtacatt
     3841 tttcgtagga ctaagtctaa aactataaaa aagaaaatga agtagtgagc cttttctttt
     3901 gtaggacaac ttttcgttgt cccatttttt acaaaaaaaa gagataacaa ataaacgcta
     3961 tctctttaaa aatcttattt ctg
//
LOCUS       DAOSMY010000125         3958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_22970_length_3958_cov_3.021009, whole genome shotgun sequence.
ACCESSION   DAOSMY010000125 DAOSMY010000000
VERSION     DAOSMY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22970_length_3958_cov_3.021009"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLD76_03845"
     CDS             complement(<1..923)
                     /locus_tag="PLD76_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27168.1"
                     /translation="MKAVLFILLAIAVVAIISLLVLLFIRKNTVKKEIMLRFAIIYIF
                     ILIAFGGVIFRIVHLQYVEGEKLRALTASQQSALRPIKPNRGNILASDGRLLASSIPT
                     YYLYMDLMADALVKKSTKNEAEFKQDVRRLSVALAKKFKDKTAWEYEKQFLNTFYDQT
                     NKRKREYLLYPKPISYIDLAEVKTFPILQKGRYGGGFYTKKRVTRIKPFGELASRTIG
                     DIYGEEEKGGSSGLEKQYNKLLQGEEGFYYRRKMAGKFVDIVVKDAVNGYDVVSTIDL
                     DIQDIVDSELRKKLIEIDAQSATAIVMEVQTG"
     gene            complement(928..1302)
                     /locus_tag="PLD76_03850"
     CDS             complement(928..1302)
                     /locus_tag="PLD76_03850"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOU27169.1"
                     /translation="MAKIKTLKKKIKESEEFNDLTKTSVKDIFDGNVFSKSYFLKQIK
                     LIVLVSFFIFFYMDNRMECEKQIATINDLNQQLTDVKYTSMVVEADLLSISRRTQIEA
                     LLHQHAIELKELSIPPYKLTTK"
     gene            complement(1307..2209)
                     /gene="rsmH"
                     /locus_tag="PLD76_03855"
     CDS             complement(1307..2209)
                     /gene="rsmH"
                     /locus_tag="PLD76_03855"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU27170.1"
                     /translation="MTTYHTPVLLKESIEGLLTNKSGVYVDLTFGGGGHSRFLLSQLH
                     SDAKVFGFDQDKDAEMNAVNDSRFTFVQSNFCYLENFMQYYGINEVDGILADLGVSSH
                     HFDDAERGFSFRFDGTLDMRMNQAAKLTAKEILNTYTEQQLADIFFYYGELPLARKIA
                     RKIVEQRKNEQFETIFGFVGIIQPLIGKEKEKKQLAQVFQALRIAVNNEMDALKKMLL
                     SSAKVLKKEGRFVCITYHSLEDRLVKNYLKTGNMEGIINKDFFGNIQTPFSLINNKVI
                     IPTTEEIASNSRARSAKLRIASKK"
     gene            complement(2301..2810)
                     /locus_tag="PLD76_03860"
     CDS             complement(2301..2810)
                     /locus_tag="PLD76_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU27171.1"
                     /translation="MQNVVKSSNFTLQEEQDMTSLIGNIEAKVDAKGRAFVPAQFRRQ
                     LQDETKFILRKGLFHDCLVLYTEQAWNDIITVLRNNLSRWNRREEQIFRQFVAEADRI
                     ELEENGRMLIPKRYIDALQIDKDIRFVGADTVIEIWPINKVENTFIDADNFALEIENL
                     MNIGKSTKD"
     gene            2930..3769
                     /locus_tag="PLD76_03865"
     CDS             2930..3769
                     /locus_tag="PLD76_03865"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU27172.1"
                     /translation="MRMGSMLDMSQPVQIDIASILKGKTGGKKPSSMLVYLLSELLHV
                     KEINHFLTLAKDKKNLDFFQALIEYLNVRVLVSGVDNLPLSGTPCVFVSNHPLGGIDG
                     VAVAYEVGKHYPEGIIIPANDLLMYLKNVQDLFIPVNKVGGQAKNLAEALHVAYMSSK
                     QMMMFPSGMVSRKSKGKIEDPEWKKNFIVKAVEYKRNIVPIYIGARNSALFYWVARVR
                     KKLKITLNIEMLLLPHEMFRQRNKELKIVIGKPIQWNVFDDAKTPHKWAQYVKKIVYN
                     LKK"
     gene            3778..>3958
                     /locus_tag="PLD76_03870"
     CDS             3778..>3958
                     /locus_tag="PLD76_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HOU27173.1"
                     /translation="MEDIIKPIDRALLKAELTSERLLRKTNKGNNEVYLITAKEAPNC
                     MMEIGRLRELSFRTAG"
BASE COUNT         1192 a          682 c          643 g         1441 t
ORIGIN      
        1 cctgtttgca cttccattac aatagcagta gccgattgtg cgtctatttc tatcaatttt
       61 ttcctcagtt cgctatccac aatatcttgg atgtctaagt caatggtgct tacaacgtca
      121 tacccattca cagcatcttt caccacaata tcaacaaatt ttccagccat ttttctgcga
      181 tagtagaatc cttcttcccc ttgcaaaagt ttgttgtatt gtttctctaa gccactacta
      241 cctccttttt cttcttcccc atagatgtct ccaatagtac gtgaggctag ttctccaaaa
      301 ggtttgatgc gggttactcg tttttttgta taaaaacctc caccatatct tcctttttgc
      361 aaaattggga acgtttttac ttcagctaaa tcaatatatg atatgggttt aggatacaac
      421 agatattctc ttttacgttt gtttgtttgg tcgtaaaaag tgtttaaaaa ttgtttctca
      481 tattcccatg ctgttttatc tttaaatttt tttgctaatg ctactgataa acgtctaacg
      541 tcttgtttaa attctgcttc attctttgtg ctttttttca ccaaagcatc agccattaaa
      601 tccatataca aataatacgt aggaattgag ctcgctaaaa ggcgtccatc actcgctaaa
      661 atattacctc tgtttggctt aattggtcgc aatgcagatt gttggctagc tgttagtgcc
      721 cgtaattttt ctccttccac atattgtaga tgaacaattc taaatataac tcctccaaat
      781 gcaattagaa taaaaatgta gataattgca aaacgaagca taatctcttt ttttaccgta
      841 tttttacgta taaacaataa caccagcaag ctaatgatag caactactgc aattgcaagt
      901 aatataaaca aaaccgcttt cattgtgtta ttttgttgtt aatttatacg gtggaatact
      961 tagttctttt aactcaatag catgttggtg taataatgct tctatttgtg ttcttctact
     1021 tatactaagc aaatcagctt ctactaccat tgatgtatat tttacatcag ttaattgttg
     1081 atttaaatca ttgatagttg caatttgttt ttcacattcc atacggttat ccatgtaaaa
     1141 aaatataaaa aaactaacca atacaattaa tttaatttgt tttaaaaaat agcttttgga
     1201 aaatacattt ccatcaaaaa tatctttaac cgaagttttt gttaaatcgt taaattcttc
     1261 gctttccttt attttctttt ttaatgtttt tatttttgcc atactgctat tttttgcttg
     1321 ctatgcgtag ttttgcactt cttgctcgtg aattgcttgc tatttcttcg gtcgttggaa
     1381 taattacttt gttgtttatc aggctaaatg gcgtttggat attcccaaaa aagtctttgt
     1441 ttataatccc ttccatattg cctgttttta aataattttt caccaatcga tcttctaatg
     1501 aatgataggt aatgcaaacg aaacgacctt ctttctttaa aacttttgca ctactcagta
     1561 acattttttt taatgcatcc atttcgttgt taacggctat tcgtaatgcc tgaaatactt
     1621 gtgccaattg ttttttttcc ttttcttttc ctatcagagg ttgtattatg ccgacaaatc
     1681 caaaaatggt ttcaaattgt tcgttttttc gttgctctac tatttttctc gctatttttc
     1741 ttgccagagg taattctcca taataaaaga atatatctgc aagttgttgt tctgtgtatg
     1801 tgtttaaaat ttcttttgct gtaagttttg cagcttgatt catgcgcata tctaatgttc
     1861 catcaaaacg gaatgaaaaa ccgcgttctg catcatcaaa atgatgagaa gacaccccta
     1921 aatcagctaa tattccgtcc acttcgttaa tcccatagta ttgcataaaa ttttctaagt
     1981 agcaaaagtt gctttgtaca aacgtaaatc ggctatcatt tactgcattc atttctgcat
     2041 ccttatcttg gtcaaatcca aatacttttg catcgctatg tagttgactg agtaaaaaac
     2101 gagaatgacc acctccacca aacgttaaat caacatatac ccccgatttg ttagtgagta
     2161 gaccttcaat actttctttt aaaagaacgg gagtgtgata cgttgtcatt ctatattttc
     2221 gtttttttat ttgttaaccc ttattatata tttgcgatta ttgtatatat atgtgcgaaa
     2281 tattgtctct tcgcaatttc ttaatctttc gtagatttgc caatattcat caaattttct
     2341 atttcaagtg caaaattatc cgcatcaata aaggtatttt caactttatt aataggccaa
     2401 atttcaatga cggtatctgc tccaacaaaa cgaatatctt tgtcaatttg taaggcatca
     2461 atataccgtt ttggaataag cattcgcccg ttttcttcta attcaatgcg atcggcttcg
     2521 gctacaaact gtcgaaatat ttgctcttca cgtctgttcc atctgcttaa gttgtttcgc
     2581 aacacagtaa taatatcatt ccaagcttgt tctgtataca ataccaagca atcatgaaac
     2641 aatcctttgc gtaaaatgaa tttggtttca tcttgcaatt gtcttctgaa ttgggctgga
     2701 acaaaggctc tgccttttgc gtcaactttt gcttctatat ttccaattaa tgatgtcata
     2761 tcttgctctt cttgaagtgt aaaattacta ctttttacga cattttgcaa ctttttacga
     2821 cacaattccc cacaaagtta ttaacaggct ttattttata gattgttata aaaaaattgt
     2881 gtattcaatc tttgggtgga aaaaaaaatg tatatttgtc ggcattataa tgagaatggg
     2941 tagtatgttg gacatgagtc agccagtgca aatagatatt gcatcaatat taaaaggaaa
     3001 gactgggggg aaaaaacctt cttcaatgtt ggtttatttg ttgtctgagt tattgcatgt
     3061 gaaggaaata aatcattttt taactttagc taaggataaa aaaaatttgg acttttttca
     3121 ggcattaata gagtatctca atgtgcgtgt tttagtttca ggagtcgata atttgcctct
     3181 atcgggtacg ccttgtgtgt ttgtgtccaa tcatccatta ggtggtatag atggcgtagc
     3241 agttgcgtat gaggtaggta agcattatcc tgaggggata attatcccag ccaatgattt
     3301 gttgatgtat ttaaaaaatg tacaagattt gtttatcccg gttaataagg tgggagggca
     3361 ggcaaaaaat ttggcagaag ccttacatgt ggcatatatg tcatctaagc aaatgatgat
     3421 gtttccgtca ggaatggttt cgcgtaaatc gaaaggaaaa atagaagacc ctgagtggaa
     3481 aaaaaatttt attgtaaaag cagtagagta taaacgtaac atagtaccta tatatatagg
     3541 agcacgtaat tcagctttgt tttattgggt agcacgtgtg cgtaaaaaat tgaaaataac
     3601 ccttaatata gaaatgctat tgttgccaca tgaaatgttt cgccagagaa ataaagagtt
     3661 gaaaattgtt ataggtaaac ctattcaatg gaatgtgttt gatgatgcaa aaacacctca
     3721 taagtgggca caatatgtaa aaaaaatagt gtataattta aaaaaataac caaaactgtg
     3781 gaagatatta taaagccaat agacagagct ttactaaaag ccgaattaac ctccgagaga
     3841 ttgttgagaa aaaccaacaa agggaataac gaggtttatt taataacggc aaaagaggct
     3901 ccaaattgca tgatggagat aggtcgtttg cgtgagttgt cgtttcgtac agctggtg
//
LOCUS       DAOSMY010000126         3949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_23031_length_3949_cov_2.725989, whole genome shotgun sequence.
ACCESSION   DAOSMY010000126 DAOSMY010000000
VERSION     DAOSMY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23031_length_3949_cov_2.725989"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PLD76_03875"
     CDS             <1..1168
                     /locus_tag="PLD76_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU27174.1"
                     /translation="VVDTTVTVNTGNGLKQFGKRTMRDWNANKGGFGVMSMPKILYQS
                     SNVGTSSIIYDHYAHNPQRFIDLLKRTKIHQAGDLGIPGHGHVKIKDPSMKTWYNTTL
                     PWMSIGYEIQLPPIYTLMYYNAFANNGQMMKPYFTKEIRQHGVTVEQFDPIVINPKIC
                     SDATLGKIQSMLEGVVTQGTGKSIRSKLFSIAGKTGTAQIAIDGKYKDASGKARHQVS
                     FCGYFPANKPMYSMIVYIKNPMGAPASAGGMSGAVFKNVAEKVYTMKLGDIPSWAHSE
                     VTDSLAIIKKGYVPHINEVLKTMAKPIKMADKTMQWGEYIVNDSTQIASFNPFVMRKH
                     TMPNTIGMSAKDAVYIVEKMGLKVTIIGFGKVVEQTIPENEHIVQGMPVTLTLN"
     gene            1179..2654
                     /locus_tag="PLD76_03880"
     CDS             1179..2654
                     /locus_tag="PLD76_03880"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU27175.1"
                     /translation="MKTLDELVKNIAVIRRLHYTSDRQINGLCFDSRQVQQGYLFFAT
                     KGSAVDGHQYIADAIELGATAIVCEQLPTSIHSDIAYLQVADSAVAMGNMAANWYDNP
                     SQKLTLVGVTGTNGKTTIATLLYNLFEQLGYVSGLLSTVCNYVHKEQIPSTHTTPDAL
                     SIQALLHRMVIAGCSYAFMEVSSHASDQKRTAGLHFAGGIFTNLTRDHIDYHQTMEAY
                     LKAKKTFFDLLPKTAFALTNTDDKNGLVMLQNTAANKYTYSTQTLADFKGKIIELSFE
                     GMLLSINNKELSVAFVGKFNVSNLLAVYGTAVLLGIHPDEALVALSTLKPVSGRFETL
                     QSPTGFTAIVDYAHTPDALNNVLETINDIRSEGIAENMGKLYTVVGCGGNRDKGKRPI
                     MAKEAVKASNMAIFTSDNPRNENPKDILNDMVAGINEEEKKSTLVIEDRYQAIKTACM
                     LAQRGDIILVAGKGHETYQEIAGVKHHFDDKEVLAELFAQL"
     gene            2668..3894
                     /gene="mraY"
                     /locus_tag="PLD76_03885"
     CDS             2668..3894
                     /gene="mraY"
                     /locus_tag="PLD76_03885"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU27176.1"
                     /translation="MLYYLFRFLEENFNLPGAGLFSYVSFRAGMALIFSLFISMVIGK
                     NIINYLRKKQIGESIRDLGLDGQMSKKGTPTMGGIIIIIAILVPSLLFGRIRNIYLLL
                     MIATTVWLGVIGFLDDYIKVFRKHKEGLNGKFKIVGQIGIGLLVGMTMFLSPEVVMIE
                     NTNHVKVQHIETTELDSKSIAVKSTQTTIPFIKNTNFDYADFLPFLKEHTQTGAWILF
                     IIITIFIVTAVSNGANLTDGLDGLAAGSSSIIGITLGVLAYVSSHVGFAGYFDIMFVP
                     GSEELVVFASAFVGATVGFLWYNSYPAQVFMGDTGSLTIGGIIGVFAILIHKEFLLPI
                     LCGIFLIENLSVMMQVSYFKYTKKKYGEGRRIFLMSPLHHHYQKKGIPEAKIVTRFWI
                     IGIALAIFTLITLKIR"
BASE COUNT         1397 a          662 c          768 g         1122 t
ORIGIN      
        1 cgtggtagat actactgtta ctgtaaatac aggcaatgga ttaaaacaat ttgggaaaag
       61 aaccatgcga gactggaatg ccaacaaagg gggatttggt gtaatgagta tgccaaaaat
      121 attgtatcaa tcatctaatg tagggacatc cagcattata tacgatcatt atgcccataa
      181 tccacaacgc tttattgatt tactaaaacg caccaaaata catcaggcag gagatttagg
      241 aatacctgga cacggacacg taaagattaa agacccaagc atgaaaacat ggtacaacac
      301 aactctgcct tggatgtcaa taggatacga aattcaatta cctccaattt atacacttat
      361 gtactataat gcatttgcaa ataatggaca aatgatgaaa ccttatttca ctaaagaaat
      421 caggcaacat ggcgttacgg tggagcaatt tgacccaatc gttatcaatc caaagatttg
      481 ttcggatgca acattaggca aaatacaatc catgctagaa ggtgtggtaa cacagggaac
      541 tggcaaaagt attcgatcaa aattattttc tattgcagga aaaacgggta cagcccaaat
      601 tgctattgat ggcaaataca aagatgctag tggaaaagca agacaccaag tatctttttg
      661 tggatatttc cctgcaaata aaccaatgta ttcaatgatt gtatacataa aaaatccaat
      721 gggggctcct gcttctgctg gaggaatgag cggtgcagtg ttcaaaaatg tagcagaaaa
      781 agtatacaca atgaaactag gagatatacc ttcttgggct cattcggaag taacggattc
      841 tttagcaatc ataaaaaaag gatacgtacc acacattaat gaagtactta aaaccatggc
      901 aaagccaatt aaaatggcag ataaaactat gcaatgggga gaatacattg tgaatgactc
      961 tacacaaata gcctcgttta atccttttgt tatgaggaaa cacactatgc caaatactat
     1021 tggtatgagt gctaaagatg ctgtgtatat agtagaaaaa atgggactaa aagtaacaat
     1081 tattggtttt ggaaaagtag tagagcaaac aataccagaa aacgaacaca tcgtacaagg
     1141 tatgcccgta acacttacat taaactaaaa atgagaaaat gaaaacgctt gacgaattag
     1201 taaaaaacat agcagttatt agacgtttgc actacacttc tgatagacaa ataaatggat
     1261 tgtgtttcga ttcgagacaa gttcaacaag gatacctatt ttttgctacg aaagggtcag
     1321 ctgtagatgg gcatcaatac attgcagatg ctattgaatt aggagccaca gctattgttt
     1381 gcgaacaact accaacatcc attcatagcg atattgcata tttacaagtt gctgatagtg
     1441 ctgtagctat gggtaatatg gcagctaatt ggtatgataa tccttcacaa aaactaactc
     1501 ttgtaggtgt aacaggtacc aatggaaaaa caactatagc cactttgcta tataatctat
     1561 ttgaacaatt aggctatgtt tctggattac tttctacagt atgtaattat gttcacaaag
     1621 agcaaatacc aagtacacac accacgcccg atgctcttag catacaagct ttattacata
     1681 gaatggttat agcaggatgt tcgtatgcat ttatggaagt aagttcgcac gctagtgatc
     1741 aaaaacgcac tgctggatta cattttgcag gaggtatttt tacaaatctt acacgcgacc
     1801 atatcgatta tcatcaaaca atggaagcct atttaaaagc caaaaaaaca ttttttgact
     1861 tgttgccaaa aacggctttt gcacttacaa atactgatga taaaaatgga ttggtcatgt
     1921 tgcaaaatac tgctgcaaac aaatacacct attcaaccca aacattagct gactttaaag
     1981 gaaaaatcat tgagctaagc tttgagggca tgttactttc tatcaataac aaagagctat
     2041 cagtagcatt tgtaggcaaa tttaatgttt caaatttact agctgtatac ggaacagcag
     2101 tattacttgg catacacccc gacgaagctc tcgtcgcttt gagtacacta aaacctgtaa
     2161 gcggtcgttt tgaaacgcta caatctccta ctggatttac agctattgta gattatgcac
     2221 acacacctga tgcattgaac aacgtattag aaacaatcaa tgatatccga tcagaaggaa
     2281 tcgctgaaaa tatgggaaaa ctatacacag ttgttggttg tggtggtaat agagataaag
     2341 gtaaaagacc cataatggca aaagaagcag taaaagcaag caacatggca atctttactt
     2401 cagataaccc tcgcaatgaa aaccccaagg atattctgaa tgatatggta gcaggaataa
     2461 atgaagaaga aaaaaaatca acactagtaa ttgaagatag gtatcaagcc ataaaaacag
     2521 cctgtatgtt agctcaacgt ggagatatta ttctggtggc aggaaaagga cacgaaacgt
     2581 atcaagaaat agcaggcgta aaacatcatt ttgacgacaa agaggtatta gcggaactat
     2641 ttgctcaact ataaaacaaa gtagcgtatg ttgtactatt tatttagatt tttagaagaa
     2701 aactttaact tacctggtgc aggtttgttt agttatgtat cattccgtgc aggcatggca
     2761 ctcattttct cgctctttat ttcgatggtt atcggaaaaa atatcatcaa ctatttgcgt
     2821 aaaaaacaaa taggagaatc cattcgtgat ttagggcttg atggacaaat gagcaaaaaa
     2881 ggaacaccca ctatgggagg gattattatc attatagcca tcttggttcc tagtttattg
     2941 tttggtcgca tacgtaatat atatttactt ttgatgatag caactacagt atggttggga
     3001 gttatcggtt ttctcgacga ctatattaaa gtgttcagaa aacacaaaga gggattaaac
     3061 ggaaaattca aaattgttgg gcaaattggc attggactat tagtgggtat gaccatgttt
     3121 ttaagtcctg aagtagtaat gatagaaaat accaatcacg ttaaagtaca acatatagaa
     3181 acaaccgaat tagactcaaa aagcatagct gtaaaatcga cacaaaccac tattccattt
     3241 atcaaaaata caaactttga ttatgctgat tttttaccat tcttaaaaga acacacacaa
     3301 acaggagcat ggattttatt tattataatt actatattta ttgtaacagc agtgtcaaat
     3361 ggagcaaacc taacggatgg attagatgga cttgctgctg gttcgtctag tatcattggt
     3421 attacactgg gcgtattagc atatgtatct agccatgtgg ggtttgctgg gtattttgac
     3481 atcatgtttg tgcctggaag cgaggaatta gtagtatttg caagtgcttt tgtgggagcc
     3541 acagttggtt ttttgtggta caactcctac cctgctcagg tatttatggg cgacacagga
     3601 agtttaacaa ttggaggcat tattggcgtg tttgcgatac tcatacacaa agaattttta
     3661 ctaccaatct tatgtggcat tttcttaata gaaaacctgt cggtaatgat gcaagtatcg
     3721 tactttaaat atacaaaaaa gaaatatgga gaagggagac gcatattttt aatgtcccct
     3781 ttacatcatc attatcagaa aaaaggcatt cctgaagcta aaattgtaac tcgtttttgg
     3841 attattggta ttgcactggc aatatttaca cttataacac ttaaaattcg ctaaatatga
     3901 aaaagatagt gatacttggt ggaggagaaa gcggagtagg tgctgctgt
//
LOCUS       DAOSMY010000127         3911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_23319_length_3911_cov_3.386411, whole genome shotgun sequence.
ACCESSION   DAOSMY010000127 DAOSMY010000000
VERSION     DAOSMY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23319_length_3911_cov_3.386411"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1832)
                     /locus_tag="PLD76_03890"
     CDS             complement(<1..1832)
                     /locus_tag="PLD76_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU27177.1"
                     /translation="MIREDEHEQTYVEVFGAKEHNLKNVDVKIPHNSLTVITGLSGSG
                     KSSLAFDTIFAEGQRRYVETFSAYVRNFLGNIERPNVDEIKGLSPVIAIEQKTTNRNP
                     HSTVGTVTEIYDFLRLLYARAGVAYSYITGEKMVKYTEEQILSLLLQQYSGKNVYLLA
                     PVVRTRKGHYKELFEQIRKKGYLNVRIDGELREIVHGLKLDRYKNHDIEVVIDRLEVA
                     AKHEKRLKDSLHIAITQGDGIVMVLDKNSDEIRFFSKKLMCPTTGISYNEPAPHDFSF
                     NSPKGCCPKCNGVGVITKIDSSKIIPDDSLSIYDGAIAPLGKFKNGILFLQIQTLCEQ
                     YDCTLKTPIKKLPPELVDKIIYGTSDKLFIKSETAGLSNYPIAFDGIVHYVTMQQDSD
                     ASATAQKWAEQFSETVVCPECNGTRLKKEALFFKILDKNIAELSAMDIEQLHSWIQLN
                     TQHFDERQKVIATEICKEITTRLQFLLNVGLTYLSLNRPSMSLSGGESQRIRLATQIG
                     SQLVNVLYILDEPSIGLHQRDNLKLIDSLKQLRDMGNTVIVVEHDKDMMLAADYVVDL
                     GPKAGRLGGELVFAGTPSEMLRAHTLTSDYITGVRKIEIPPKRR"
     gene            complement(1829..2584)
                     /locus_tag="PLD76_03895"
     CDS             complement(1829..2584)
                     /locus_tag="PLD76_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27178.1"
                     /translation="MQHIKPYILFFVFLLVFFSCTKEKTIDTPPTHETNSITQTNVLE
                     KVLGTYSLFDRNWNLMEYTIFQEDSVFKAKITYNKKTSVTLLRLVDLSSNLYSFEYDG
                     KTYYLRVDNILAEKRTIRILNEREERFLNFFEGKGFVDLSEQENRKNQTLRRKLDISS
                     FFSPIRPTKKNWSSDMNYTDTLQYISFDDVSNPMRAIFTTNTRDTVYLSFEIPIGSEF
                     NGSTMEVRWKIDTVLVNKLPKLNQKLLHYRVVR"
     gene            complement(2589..3326)
                     /gene="pssA"
                     /locus_tag="PLD76_03900"
     CDS             complement(2589..3326)
                     /gene="pssA"
                     /locus_tag="PLD76_03900"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOU27179.1"
                     /translation="MSFTIKKHIPNTITCFNLFSGAIAVAFAFEGNLLFATLFIFLSA
                     VFDFLDGFLARLLGAYSELGKQLDSLADLVSFGLAPSVIVFSLFKEFPYPEAMFDYAF
                     LFPYTGFFIVVFSALRLAKFNIDESQTTSFRGLPTPANAILIGGIALSQFSFWTIPVV
                     GLYLLLALVCVSCFLLVSPLPMFSLKMKGFSIANNKVQYTFLLLSLVLLVAFFFFGFA
                     LSICLYILMSVVLAIKNRLSNKKDLHI"
     gene            complement(3339..>3911)
                     /locus_tag="PLD76_03905"
     CDS             complement(3339..>3911)
                     /locus_tag="PLD76_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU27180.1"
                     /translation="PKDRTTIINLLEQISHTSLYPENKKLAQEVLYRLKKAHVGSKPN
                     VNELRTIEGKTITFSDFAGKYVYIVFASSLINETYADIIYLDKLRKKYAENLEVVFIF
                     AQETEAKTIAYTKEIGADLIFCNWNSDAQLIDAFGVQNIPSYYLLDREGFFSHSPALS
                     PSQGFERKLDAMLEVEKAAEGSSTMNKPFF"
BASE COUNT         1256 a          776 c          613 g         1266 t
ORIGIN      
        1 tcgcgtcttt tagggggtat ttctattttc ctaactcctg taatataatc agaagttaag
       61 gtatgtgcac gcagcatttc cgacggagtt cctgcaaaaa ccaactcacc acctaatcgt
      121 ccagctttgg gacctaaatc aaccacataa tcggctgcca acatcatgtc tttgtcgtgt
      181 tctacaacaa tgacggtatt tcccatatca cgtaattgct ttagcgagtc tatcagtttt
      241 aaattatctc gttggtgcaa tccaatactg ggctcgtcta aaatatacag cacattgact
      301 aattgagacc caatctgcgt tgccaaccgt atgcgttggc tttcgcctcc cgacaaactc
      361 atggaaggac gattaagaga gaggtaagta agtcccacat ttaataaaaa ctgtaacctt
      421 gtggtaattt ctttacaaat ttctgtggca attacttttt gtctttcatc aaaatgttgt
      481 gtgttgagtt gtatccaact atgcaattgc tcaatatcca tagcagacaa ttcagcaata
      541 tttttatcca aaattttgaa aaacaaggct tcttttttaa ggcgtgtgcc attacactct
      601 ggacatacga ctgtttctga aaattgttca gcccactttt gtgcggttgc agaggcatcg
      661 ctatcttgtt gcatggttac gtagtgcaca atgccatcaa aggcaatagg ataattagat
      721 aagcctgctg tttcgctttt gataaataat ttatcggatg tgccataaat aattttatcc
      781 actaattctg ggggcaattt ttttataggt gttttcagcg tacaatcgta ttgttcgcat
      841 aatgtctgaa tttgtaaaaa taatatccca tttttgaatt ttcctaaggg tgcaatggca
      901 ccatcataaa tagaaagaga gtcgtcagga ataattttac tactatctat tttggtaata
      961 acacctaccc cattacattt tgggcaacag cctttgggcg aattaaaaga aaaatcgtga
     1021 ggagcaggtt cattgtacga aattccagta gtcggacaca ttaatttttt gctaaaaaat
     1081 ctgatttcat cgctattttt atctaatacc ataacaattc catcgccttg ggttatcgct
     1141 atatgtagcg aatctttcaa tcttttttcg tgcttggcag ctacttccaa tcggtcaatg
     1201 acgacttcaa tatcatgatt tttgtatcta tcaagtttta atccatgtac aatttctctc
     1261 aattcgccgt ctatccgcac attcaaatag cccttctttc ttatttgttc gaacaactct
     1321 ttgtaatgtc ctttgcgagt tctaacgaca ggagctaaca aatacacgtt tttcccacta
     1381 tattgctgta aaagcaatga aagaatctgc tcttcggtat attttaccat tttctcgcca
     1441 gtgatatacg aatacgccac accagctcgt gcatacaaaa gacgtaaaaa atcgtatatt
     1501 tcggtaaccg tacccacagt agaatgtgga ttgcgattgg ttgttttttg ttctatggca
     1561 ataaccgggc tcaaaccctt tatttcatca acattcggac gttcgatatt gcccaaaaaa
     1621 ttacgtacat aggcagagaa tgtttctaca tagcgtcttt gtccttccgc aaatatggta
     1681 tcaaacgcca aagaagattt accacttccg ctcagtccag taattacagt taggctattg
     1741 tgaggtattt taacgtctac atttttgagg ttatgttctt tcgctccaaa aacttctaca
     1801 tacgtttgtt catgttcatc ttctcgtatc atcgtactac tctataatgc aacaactttt
     1861 ggtttaattt aggtaattta ttcaccagca ctgtatctat cttccacctc acttccatcg
     1921 tgcttccatt aaactccgac ccaataggta tctcaaagct taaataaaca gtatcccttg
     1981 tatttgttgt aaaaatagct ctcatgggat tggatacatc atcaaaagaa atatactgta
     2041 aggtatcagt ataattcata tcactactcc aatttttctt tgttggacga ataggagaga
     2101 aaaaagaaga tatatctaat ttcctgcgta acgtttgatt tttcctattt tcttgctcac
     2161 tcaaatcgac aaaacctttc ccttcaaaga aatttaaaaa ccgctcttcc ctttcattca
     2221 aaattcgtat tgttcgtttc tctgccaata tattatctac acgtaaataa taggttttac
     2281 cgtcatattc aaatgagtac aaattgctcg acaaatctac taaccgtaaa agcgtaacag
     2341 atgttttttt attatacgta atttttgcct taaatacgga atcttcttga aaaatagtgt
     2401 actccatcaa gttccaattc ctatcaaaca acgaataggt tcctagcacc ttctccaaca
     2461 catttgtttg tgtaatactg tttgtttcgt gggttggtgg tgtgtctatt gttttttctt
     2521 tggtacaaga aaaaaacact agaagaaaca caaaaaatag tatatatggt tttatatgtt
     2581 gcatcaatct atatatgtaa gtctttctta ttcgatagac gattttttat tgcaagtaca
     2641 actgacataa ggatatacaa acagatagac aacgcaaagc caaagaaaaa gaatgcaaca
     2701 agtagcacaa gtgaaagcaa taaaaaagta tattgcacct tattgttagc aatactaaat
     2761 cctttcattt tgagagaaaa cataggtaaa ggcgatacta gtaaaaaaca agatacacaa
     2821 accaacgcta gtaataaata taaacccaca acaggtattg tccaaaatga aaattggctt
     2881 aacgcaattc caccaattaa tatcgcattc gctggcgtag gcaagcctct aaaactggtt
     2941 gtttgagact catcaatatt aaacttagcc aatcgtagtg cagaaaatac aacaataaaa
     3001 aaacctgtat aaggaaataa aaaagcataa tcaaacattg cctctggata cggaaattct
     3061 ttaaacaaag aaaacacaat aacactaggt gctaatccaa agctcaccaa atcggctaac
     3121 gaatcgagtt gtttgcctaa ttctgaatat gctccgagca aacgagccag aaaaccatcc
     3181 agaaaatcga atacagctga aaggaaaata aataatgtag caaacaataa attaccctca
     3241 aaagcaaagg ctactgcaat ggcaccagag aataaattga aacacgttat cgtatttggg
     3301 atatgttttt taatagtaaa tgacatatgg tataatgatt aaaaaaatgg tttattcata
     3361 gtagacgaac cctcagcagc tttttctact tccaacatag catcgagttt acgctcaaaa
     3421 ccttgcgatg gacttaatgc tggactatgg ctaaaaaaac cttctctatc caacaaataa
     3481 taggaaggaa tattttgcac accaaatgca tctatcaatt gggcatcact attccaattg
     3541 caaaaaatta agtccgctcc tatttccttt gtataggcta ttgttttcgc ctcagtttct
     3601 tgtgcaaaga taaatacaac ttctaggttt tcagcatatt ttttacgcaa tttatccaaa
     3661 taaataatat ctgcataggt ttcgtttatc aacgaactag caaaaactat atacacatac
     3721 ttccctgcaa aatccgaaaa cgtaattgtt tttccttcaa tagtacgtaa ttcattcaca
     3781 ttgggcttac ttccaacatg ggcttttttc aacctataca acacttcctg tgccaatttt
     3841 ttattttctg gatacaagct tgtatggctg atttgttcca ataaattgat aatagttgtt
     3901 ctatctttcg g
//
LOCUS       DAOSMY010000128         3870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_23616_length_3870_cov_3.692267, whole genome shotgun sequence.
ACCESSION   DAOSMY010000128 DAOSMY010000000
VERSION     DAOSMY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23616_length_3870_cov_3.692267"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLD76_03910"
     CDS             <1..825
                     /locus_tag="PLD76_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOU27181.1"
                     /translation="TVISISLVLFLIGITSLLVLLANQLSIHVRENLAISIVMKDNVN
                     EVEIQKLQKMLDATQYVKSTEYIDKETAAKELAAELGEDPIAFLGYNPLLASLEVKLH
                     SEYTHNDSIAKIETELKNFPEIKEVIYQKNLMQAVNENVSRITVFLLGISILLLFISF
                     ALINNTVRLSIYSKRFLIHTMKLVGATGSFIRQPFMIQGIFTGIVSACIAMGLLSLLV
                     VSFQTELGGIITLESIDFMLVSFVVMIAVGIIITFVSTYFAVNRYLRIKLNDMYYM"
     gene            851..1093
                     /locus_tag="PLD76_03915"
     CDS             851..1093
                     /locus_tag="PLD76_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOU27182.1"
                     /translation="MKKEGDLSKRLGRINLKLIFIGVVVIIIGLVLMSVGGISTEEVF
                     AEDIFSVRRIVVAPMMVFFGFLFLVFAILYTPKTKA"
     gene            1090..1947
                     /locus_tag="PLD76_03920"
     CDS             1090..1947
                     /locus_tag="PLD76_03920"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOU27183.1"
                     /translation="MSIVETVIIAIVEGLTEFLPVSSTGHMIITQALLGVESTEFVKA
                     FTVNIQFGAILAVVVLYWQRFFKLNDTSSNYSIANQSKWKQLLYKFDFYIKLFVAFLP
                     AACIGFFAGEYIDMMLESVEVVAVMLLVGGIFMLFIDKLFNKKSDNQAITYKRAWIIG
                     FAQCIAMIPGVSRSMASIVGGLATKLDRKNAAEFSFFLAVPTMAAAAGYTLMKLVMDE
                     RTSAMLVDNLVVLLIGNIVAFVVAIIAIKFFIGFITKYGFKAFGWYRIIVATVLLILL
                     VANVDLAIA"
     gene            1947..2630
                     /gene="truB"
                     /locus_tag="PLD76_03925"
     CDS             1947..2630
                     /gene="truB"
                     /locus_tag="PLD76_03925"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU27184.1"
                     /translation="MNFIEGEILVFNKPLTWTSFALVHRVRAMICRKLKVKKLKVGHA
                     GTLDPLATGVMIICTGKATKRIEEFQYQTKEYIATLLVGATTPSFDLETKIDNVYPTE
                     HIKKETVQQVLAQFVGSIRQVPPVFSAVKVDGKRAYEYARNGEEVEIKAKELVIDEIE
                     LIDYSLPKITIRVVCSKGTYIRALARDIGVALQSGAHLVALERTRIGEYRIENCLTLD
                     DFQKIIDSE"
     gene            2694..3743
                     /gene="queA"
                     /locus_tag="PLD76_03930"
     CDS             2694..3743
                     /gene="queA"
                     /locus_tag="PLD76_03930"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU27185.1"
                     /translation="MKLSQFKFKLPEELIATHPTKFRDESRLLVLNKKTGEIEHKVFK
                     NVYEYFSENDVFVFNDTKVFPARMFGNKEKTGARIEVFLLRELNRELKLWDVLVDPAR
                     KIRIGNKLYFGDNDELVAEVIDNTTSRGRTLRFLYDGNYETFKQLLYSLGETPLPRSL
                     NREAEPEDAERYQTIYAKNEGAVVAPTAGLHFSRELLKRMEIKGIHFANVTLHAGLGS
                     FRDIDVEDLTKYKMDSEQMMVTNEACQIVNAAKENRQQVCAVGTTVLRSLETAVSTNG
                     MIKPFDGWTNKFIFPPYDFTVATSMISNFHMPYSMLLMLVAAFGDYDYVMHAYSEAVK
                     EKYRFGTYGDALLII"
BASE COUNT         1236 a          551 c          775 g         1308 t
ORIGIN      
        1 acagttatta gtatatcttt ggtgcttttt ttaattggta ttacctcatt gctggtttta
       61 cttgccaatc agttatccat acatgtgcgt gaaaatttgg caatatctat tgtaatgaaa
      121 gataatgtga atgaggtaga aattcaaaaa ttacaaaaaa tgttggatgc tactcaatat
      181 gtaaaatcta ccgaatacat tgataaagag actgcagcta aagaattagc agcagaacta
      241 ggcgaagatc ccatcgcttt tttgggatat aatcctcttt tagcatcctt ggaagttaaa
      301 cttcattccg aatatacaca caacgatagt attgcaaaaa ttgaaaccga actgaaaaac
      361 ttcccagaaa ttaaagaagt tatttatcaa aaaaacttaa tgcaagcagt aaacgaaaat
      421 gttagtagaa taacggtctt tttgttaggc atttctattt tattgctatt tatatcgttt
      481 gcccttatca ataataccgt gcgtttaagt atttattcaa aacgtttctt aatccatacc
      541 atgaaattag tgggggctac aggaagcttt attcgtcaac catttatgat acaaggtatt
      601 tttacgggta ttgtatccgc atgtattgct atgggattgt tgtcgttact tgtggtgtct
      661 ttccaaactg aattaggtgg aattattacc ctcgaaagta ttgattttat gctggtgtca
      721 tttgtggtaa tgattgctgt tggtattata attacctttg tgtctactta ttttgctgtc
      781 aatcgttatt tgcgtatcaa acttaatgat atgtattaca tgtaattttt acacaaaaaa
      841 aacagttaac atgaaaaaag aaggtgattt aagtaaacga ttaggccgta tcaatttgaa
      901 attaattttt atcggtgttg ttgttattat tattggattg gtattaatgt ctgtgggagg
      961 gatatctaca gaagaggttt ttgctgagga tatttttagt gtaagacgta ttgtagttgc
     1021 tccaatgatg gtattttttg gatttttatt tctcgttttt gctattttat atacacctaa
     1081 aacgaaagca tgagtatagt tgaaactgtt attatagcca ttgttgaggg gctaacagag
     1141 tttttgcccg tatcttctac agggcacatg attattacac aagccttatt gggtgttgaa
     1201 agtacagaat ttgtaaaagc atttaccgta aacattcaat ttggtgcaat tttagctgtt
     1261 gttgttttat attggcaacg tttctttaaa ctgaatgata cttcttctaa ttattctata
     1321 gctaaccaaa gtaagtggaa acagttattg tataagtttg atttttatat aaaacttttt
     1381 gtagcttttt tgccagcagc gtgtatcggt ttttttgctg gcgagtatat agacatgatg
     1441 ctagaaagtg tagaagtagt tgcagtaatg ctacttgtag gcggtatttt tatgcttttt
     1501 attgataaat tatttaataa aaaatcagat aaccaagcta ttacatataa aagagcttgg
     1561 attatagggt ttgctcagtg tatcgccatg atacctggag tgtcgcgttc tatggcatct
     1621 attgtaggcg gtttagcaac taaattggat agaaaaaatg ctgccgaatt ttcgtttttt
     1681 ttggctgtac caactatggc tgccgcagct gggtatactt tgatgaagtt agtaatggat
     1741 gagcgtactt cggctatgtt ggttgacaac ttggtcgttt tgttgatagg taatattgta
     1801 gcttttgttg tggctattat tgccatcaaa ttttttattg ggtttattac aaagtatggc
     1861 tttaaagcat ttggttggta tagaatcata gtagctactg ttttactaat cttgttagtt
     1921 gcaaacgttg acctcgctat agcctaatga attttattga aggagagatt ttggttttta
     1981 ataaaccgct aacgtggaca tcttttgctc tagtacatcg tgtgcgtgcg atgatatgca
     2041 gaaaactaaa ggtaaagaaa ttaaaggttg gtcatgcagg cacactcgac cccttagcca
     2101 ctggggtaat gattatttgt acaggcaaag ctaccaaacg tatagaagag tttcagtatc
     2161 aaacaaaaga atatattgct acgttgctag ttggagcaac tactccgtcg tttgatttag
     2221 aaacaaaaat agataatgtg tatcctactg agcatataaa gaaagaaacg gtacaacaag
     2281 ttcttgctca gtttgtgggc tcaataaggc aagtaccacc tgttttttcg gcggtaaaag
     2341 ttgatggaaa aagagcatac gaatatgccc gtaatggaga agaagtagag ataaaagcca
     2401 aagaattggt aatagatgaa attgaattaa tcgattattc gttgccaaaa attaccataa
     2461 gggtagtgtg tagtaaaggt acatatataa gggctttggc tcgggatatt ggagtagcct
     2521 tgcaaagtgg tgctcatctt gttgccttag agcgtacgcg cattggtgag tatagaatag
     2581 aaaattgctt gaccttagat gattttcaga aaataattga ttcagaataa gatatgtggc
     2641 ttcataattt gtacatatct taatagagta tagtaataac aaaaacaatc aaaatgaaac
     2701 tttcacaatt taaatttaag ttgcccgaag agctaatagc aacacatcct acaaaatttc
     2761 gcgacgaatc gcgcttattg gtgttgaata aaaaaactgg tgaaattgag cacaaagtat
     2821 ttaaaaatgt gtacgaatac ttttccgaaa acgatgtatt tgtatttaac gacaccaaag
     2881 tgtttccagc acgtatgttt ggaaataaag aaaaaacagg agcacgtatt gaggtgttct
     2941 tactaagaga attaaaccga gaacttaaac tttgggacgt attggtagac cccgctcgca
     3001 aaattcgtat tggcaataag ttgtattttg gtgataatga tgaattagtt gccgaagtaa
     3061 ttgataatac tacttctaga ggacgtactt tgcggttttt atacgatggg aattatgaaa
     3121 catttaagca attgctatat tctttgggag aaacaccact accacgatct ttgaatagag
     3181 aagcagaacc agaagatgcc gaacgttatc aaactatata tgcaaaaaat gaaggagctg
     3241 tggttgcccc taccgctggg ttgcatttta gtcgcgaatt gcttaaacgt atggaaatta
     3301 agggaataca ttttgcaaac gtaactctac acgctggatt gggaagtttt cgcgacattg
     3361 atgtggaaga tttgactaag tacaaaatgg attcggagca aatgatggta acgaatgagg
     3421 cttgccaaat tgtaaatgct gcaaaagaaa atcgtcaaca ggtatgtgct gtcggaacca
     3481 ccgttttacg ttcgttagaa acagccgtta gtaccaatgg tatgataaaa ccttttgatg
     3541 gttggacaaa taaatttatt ttccctccgt atgattttac agtagccact agcatgatta
     3601 gtaattttca tatgccatat tctatgttat taatgcttgt agccgctttc ggcgattatg
     3661 actatgtaat gcatgcatat agcgaagctg taaaagaaaa ataccgtttt ggtacttatg
     3721 gagatgcgtt gcttattatt taaagcactc attgtatgga ttatatcggt ttattttaca
     3781 gtaatatagc ttttctgtaa aaaaaaaact atatttgcac cctatttcac aaaaatataa
     3841 aacaaaagaa tattaatttt aaattttaat
//
LOCUS       DAOSMY010000129         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_23664_length_3864_cov_3.643739, whole genome shotgun sequence.
ACCESSION   DAOSMY010000129 DAOSMY010000000
VERSION     DAOSMY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23664_length_3864_cov_3.643739"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLD76_03935"
     CDS             <1..835
                     /locus_tag="PLD76_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOU27186.1"
                     /translation="SEEEMIAKYGDFIVDVVEFIRRQKHPNLFVHSKGLLHISGKEAP
                     ATWMNAEENNQPITPRTGFVVEINALWYNALKCSETIHEINGNSKQSHILHYQAELAG
                     NEFIQTFWNGMYLVDYVDGIERNMEVRPNMIFATSLPYSPLTKQQQKSILDICTKELL
                     TPKGLRSLSPKSGSYRPNYVGGMLERNRNYHNGPVWTWTLGAYTETYLKIHKKSGVSF
                     IERIVVGLEAEMKKLCIGTLSELYDGNPPYKGHGGMSFAMNVAEILRVLRLLQNIQQD
                     E"
     gene            847..2103
                     /locus_tag="PLD76_03940"
     CDS             847..2103
                     /locus_tag="PLD76_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27187.1"
                     /translation="MKALMFGWEFPPQILGGLGTASYGLTKGMAQQTDMEITFVLPKP
                     NGDEDQSFLRLVGANHVPVVYKETSNEYIEQIIGKLTRLDDYLHYRNNIRYDFSRIST
                     NELGCIYFSGRYPDNLVEEISNYEAVASVLAHSLDFDIIHSHDWLTYPAGKFAKQISG
                     KPLVVHVHATDFDRSRGNVNPTVYAIEKAGMDAADHIITVSNLTRQTVIDKYHQPPHK
                     VTTVHNAVEPLEHAETVIKAQRSEKLVTFLGRITMQKGPEYFVEAAHRVLEKTNNVRF
                     VMAGSGDMMHAMIDLVAKRNIADRFHFTGFLKGKQVHQLLADSDVYIMPSVSEPFGIS
                     PLEAMQVGTPSIISKQSGCAEILAHAIKTDYWDIDAMADAIYAIVHYPAMYKSLRNEG
                     KAEVDSITWEKAGLKVRKIYDMVLHQ"
     gene            2116..3387
                     /locus_tag="PLD76_03945"
     CDS             2116..3387
                     /locus_tag="PLD76_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOU27188.1"
                     /translation="MKNICFYFQLHLPYALKRYRFFEIGNDHYYYDDFQTEETIRLLV
                     EKSYLPANKTILDIIHHSNKAFKCAFSISGCTLEQLEQYAPEVIDSFRELAATGCVEF
                     LVEPYAHSLASVFNELEFERQVQLHAKKIEDLFGYRPTTLRNAELIYSDEIGEKAYQM
                     GYQSIVVEGGRDILGWKSAHYLYHHPHKKNLKILMRDYKLSDDITFRFSDKSWTDYPL
                     TADKYIQWIANAPTEEELINIWLGYETFGLKQDASSGILDFLKAIPYQAIVHGIEFIT
                     PTEATQKLESKDSLSAPYTSAWGSEEKDLSSWTGNDLQQEALQKLYAVTERVHLCKHK
                     TLKRDWLMIQSSDYFRYMSHRNAYNSPYDSAYDAFVNYMNILSDFLLRVDAEYPSSIE
                     NEELNALLKTITEQEKEISNLTKQLYTKNKK"
     gene            3417..>3864
                     /locus_tag="PLD76_03950"
     CDS             3417..>3864
                     /locus_tag="PLD76_03950"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOU27189.1"
                     /translation="MFSPEDELQLANKGIAKEKLETQLLNFEKGFPYAHLQQAATPGN
                     GIVVLNNEQISDYIHYYEKQLATIKALKFVPASGAASRMFKRLSEFLEAHVHNAHYLR
                     TYYPDVAAFMHHISKFAFYEELKTCIEKDGETIEDLLQKEQYNKIIL"
BASE COUNT         1347 a          718 c          748 g         1051 t
ORIGIN      
        1 atccgaagag gagatgatag caaaatacgg cgatttcatc gttgatgtag tagagtttat
       61 tcgaagacaa aaacatccca atctatttgt gcattcaaaa ggattattac atattagcgg
      121 aaaagaagct cctgccacat ggatgaatgc agaagaaaac aatcagccta ttactccccg
      181 aacaggcttt gtcgtagaaa ttaatgcttt atggtacaat gctttaaaat gttcagaaac
      241 aatacatgaa attaacggaa acagcaagca atcgcatatt ctacattacc aagcagaatt
      301 ggcaggaaat gaatttatac aaacgttttg gaacggcatg tatttggttg attatgttga
      361 tggaatagaa agaaatatgg aagtgagacc gaatatgatt tttgccacct cgctacccta
      421 ttctcctctt accaaacagc aacagaagtc gattttagac atttgcacca aagaattgct
      481 cacacctaag gggctacggt cgttaagccc taaaagtggc tcttatcgac caaattatgt
      541 aggaggaatg ctcgaacgta atcgtaatta ccacaacgga cccgtttgga catggacatt
      601 aggggcatac acagaaacat atctaaaaat acataaaaaa agcggagtgt cttttattga
      661 gcgaatcgtt gttggactag aggcagaaat gaagaaatta tgtattggca ccttatcgga
      721 actatacgat ggtaatccac catataaagg acatggaggg atgtcgtttg ctatgaatgt
      781 agcagaaatt ttaagggtat tgcggttatt gcaaaacatt caacaagacg aataaactat
      841 acgaatatga aagcattgat gtttggctgg gaatttcctc cacaaatttt gggagggcta
      901 ggtacagcga gctatggctt aacaaaaggc atggctcaac aaacagatat ggaaataacg
      961 ttcgtattac caaagcccaa tggcgacgaa gaccaaagtt ttttacgcct tgttggagcc
     1021 aatcatgtac cagtagtgta caaagaaaca tcgaatgagt atattgagca gataattgga
     1081 aaactaaccc gtttagatga ttatttgcat tatagaaaca atattcggta tgatttttcg
     1141 cgtatttcaa ccaacgaatt gggctgtatc tacttttctg ggcgttatcc agataatttg
     1201 gtagaagaaa ttagcaacta cgaagcagta gctagcgtac ttgcccatag cctcgacttt
     1261 gacataattc attcgcacga ttggcttacg tatccagcag gtaaatttgc caaacagatt
     1321 agcggaaagc ccttagtagt acatgtacat gctactgatt ttgatagaag tcgtggcaat
     1381 gtaaacccaa cggtatatgc catagaaaaa gctggaatgg atgctgcgga tcatattatc
     1441 accgtaagca acttaacccg acaaacagta attgataaat accatcaacc accacacaaa
     1501 gtaacaacgg tgcataatgc cgtagaaccg ctcgaacatg ccgaaactgt cataaaggca
     1561 caacgttcag agaaattagt tacatttttg gggcgaatca ccatgcaaaa aggaccagaa
     1621 tattttgtag aagcggcaca tagggtattg gaaaaaacca ataatgtgcg tttcgtaatg
     1681 gcaggcagtg gcgacatgat gcatgcaatg attgatttag tagccaaacg caatattgcc
     1741 gaccggttcc attttacagg atttttgaaa gggaaacaag ttcatcaatt attagctgac
     1801 agcgatgtat atattatgcc atctgtgtcg gagccttttg gcatttcgcc attagaagcc
     1861 atgcaagttg gaacaccaag tataatttcg aaacaatcgg gctgtgccga aattttagca
     1921 catgctatta aaacagacta ttgggatata gatgccatgg cagatgctat ttatgccatc
     1981 gtacactatc ctgctatgta taagagccta cgaaacgagg gaaaagcaga agtagactcc
     2041 ataacgtggg aaaaagctgg actgaaagtt cgaaaaattt acgatatggt tttacatcaa
     2101 taaaaaagac tgaatatgaa aaatatctgt ttctattttc aactacatct accctatgca
     2161 cttaaacgct atcgtttttt tgaaataggt aacgaccatt attattatga tgattttcaa
     2221 acagaagaaa ctattcgttt gctcgtagaa aaatcgtatc ttcccgctaa caaaactatt
     2281 cttgatatca tccatcattc taacaaagct tttaaatgtg ctttctctat ttcaggatgc
     2341 accctcgagc aactagaaca gtacgcccct gaagtaattg acagctttcg tgagctagcc
     2401 gcaacaggat gtgttgagtt tttagtagag ccttacgctc attcgttagc atctgtattt
     2461 aatgaattgg agtttgaacg acaagtgcaa ctccacgcca aaaagattga agatttattt
     2521 ggctatcgcc caacaacact gcgtaatgca gagcttattt attcggatga aatcggagaa
     2581 aaagcatatc aaatgggata ccaatcgata gtggtagaag gaggaagaga catactaggt
     2641 tggaaaagtg ctcactattt gtaccaccat ccacacaaaa agaacctaaa aatattgatg
     2701 cgagattata agctaagcga tgatatcact tttcgatttt ccgacaaaag ttggacagac
     2761 taccctctaa cggcagataa atacatacaa tggatagcaa acgctcctac tgaagaagag
     2821 ttaattaata tctggctagg atacgaaacc tttggtctaa aacaggatgc ttcttctggc
     2881 attttggatt ttttaaaagc tataccatat caagcaatag ttcacggtat tgagtttatt
     2941 accccaacag aagcaacaca aaaacttgag tccaaagatt cattatcggc tccttatacc
     3001 agtgcatggg gaagtgaaga aaaggaccta agtagttgga cgggaaacga tttgcaacaa
     3061 gaagcccttc agaaattata tgccgttacc gaacgtgttc atttgtgcaa acacaaaaca
     3121 ttgaaaagag actggctcat gattcaatca tccgattatt ttaggtatat gagccacaga
     3181 aatgcctata attcacccta tgattcggct tatgatgctt ttgttaacta tatgaacatc
     3241 ctgtctgatt ttcttttgcg cgtagatgcc gagtacccta gctctattga aaacgaagaa
     3301 ttaaatgcac tactcaaaac aataacagag caagaaaaag aaattagtaa tttgaccaaa
     3361 caattataca caaaaaataa aaaataatgc ctaacttcgc aaaaaaatga tgaactatgt
     3421 tttctccaga agatgaatta caattagcaa acaaaggcat agcaaaagaa aagctggaaa
     3481 ctcaattgct caattttgaa aaaggttttc catatgccca cttacaacaa gcggctacgc
     3541 ctggaaatgg aattgttgtg ttaaacaacg aacaaatttc agactatatt cattattacg
     3601 aaaaacaact agctacaatc aaagctctta aatttgtacc cgcttctggt gctgcgtctc
     3661 gtatgttcaa aagattatct gaatttttag aggcacatgt gcacaatgcc cattatttac
     3721 gtacgtatta ccctgatgta gccgctttta tgcatcatat atctaaattt gccttttacg
     3781 aagagctaaa aacctgtatt gaaaaagatg gagaaaccat cgaagatctc ttacaaaaag
     3841 aacaatacaa caaaattatt ttgt
//
LOCUS       DAOSMY010000130         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_23788_length_3847_cov_2.845464, whole genome shotgun sequence.
ACCESSION   DAOSMY010000130 DAOSMY010000000
VERSION     DAOSMY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23788_length_3847_cov_2.845464"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1106)
                     /locus_tag="PLD76_03955"
     CDS             complement(105..1106)
                     /locus_tag="PLD76_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOU27190.1"
                     /translation="MKKFFTREAKIGLLVIVALGLLYFGINYLKGINLFKPTKYFYVQ
                     YDRIDGVVKTSPVLINGYKIGHVSNIDFDYTKNAPITLEISVDKHLIVPKGSYAELYE
                     TGMLGDKAIQLVLTDNPDMHQIGDTLPGKTSGGLIGEIVNSLLPPIQKVIPQVDSTLL
                     ALRKVIESGAVENSMKNIETTTQELASMSQSLKKIVHTDVPVIMNDVSTVTQNLSKVT
                     TDLSKVDLEKTLISLNETLANLQLASSKFNEKSNTVGLLLNDPSLYLNLNATMDSSNK
                     LLIELYVYHVLSIKKRISFLNSKILTPKSFAILFCCAIFTISVVLKFSSKTTLYNPL"
     gene            complement(1117..2265)
                     /locus_tag="PLD76_03960"
     CDS             complement(1117..2265)
                     /locus_tag="PLD76_03960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU27191.1"
                     /translation="MNTLKKVIASFTFILFCTSSFAFTLVLDAGHGGQDAGAIGSFSK
                     EKNINLTYVLALGEKIAANHKDVKIIYTRTTDVFIPLNERAQIANKANADLFMSIHTN
                     SAKNKTANGTETFTLGHARSKENMELAMLENSVILLEEDYQKTYQGFDPNSTDSYIMF
                     QFMQDQYMEQSIKFADLIQQNFSKNTPLTDRGVRQAGFLVLRATTMPSVLVELGFISN
                     REEEKYLNRKENQDKMVDAIYEAFVNYKRVLEKKNGGQPIEHGKNTTIHTTPTPQQTT
                     TSTPTQKPTNTSTVVTESNILFKVQFLVSKKKYKEGNAIFKGVTNIDSYVENGFYKYT
                     TGSTSDIDAINQLKNDVAKKFPDAFVIAYKNGKRCNIHDAIKEVSDKK"
     gene            2562..>3847
                     /locus_tag="PLD76_03965"
     CDS             2562..>3847
                     /locus_tag="PLD76_03965"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOU27192.1"
                     /translation="MPTERTSTRISSKSVIEDNIVYNATDSIIILKNGIIHLHGNASV
                     TYQDIELKAAYIRLNIDSSLVFASGITDTVGTYLGAPIFKEGSETYESKSLLYNFNTK
                     KGLIFNTVTQQGEGYVSSGVTKKSTENVFCLQHGKYTTCDHHDHPHFYLSLTKAKMKQ
                     GEYIVTGPAYLVIEDLPLPLFVPFGYFPVPKTYSSGVLFPTFGDELQRGFYARNGGYY
                     FAINDYVDLALTGDVYTKGTWSLNAASSYVLRYKFRGNVNVSLMENIVGERDMPDYAK
                     SNDFRIMWSHAQDAKANPFSTLSASVNFSTSSYERNNLNSYYNPNLLSQNTRSSTITY
                     TQRFPESPFSLSASVYANQRTRDSIISLSLPNLNVSMSRIYPLKRKQVVGTDRWYEKI
                     SMTYTGRFSNSITTKEDKLLTSSFTKDWKNGVEHTLP"
BASE COUNT         1166 a          626 c          682 g         1373 t
ORIGIN      
        1 aaagattctg aagatttttc taatagcatt caaataaata atttttataa accaattaag
       61 tatgttgctc aaaatagagt taacgagatt tgggcagcag ataattatag aggattgtac
      121 agagtggttt ttgatgaaaa tttcaaaacc accgaaattg taaatattgc gcaacaaaat
      181 aagattgcaa atgattttgg agttaaaatt tttgagttta gaaatgagat tctttttttt
      241 atagacaaca catggtatac gtacaattca attagcaatt tattagaact atccatggtt
      301 gcattcaaat tgagatacaa cgatgggtcg ttcagcaaaa gccctacagt attgctcttt
      361 tcattaaatt ttgacgaggc taattgcaaa ttagctaatg tttcattaag cgaaataagt
      421 gttttctcta agtcaacctt gctcaaatcg gttgttactt ttgataaatt ttgtgtaacg
      481 gtactcacat cgttcataat tacgggaaca tccgtatgta ctatcttttt gagcgattgc
      541 gacatggatg caagttcttg tgtggtagtt tcaatatttt tcatgctatt ttctactgca
      601 ccactttcaa ttactttgcg aagagccaat aatgtagaat caacttgtgg gatgactttt
      661 tggataggag gtaataacga attaacaatt tcaccaatta atcctccaga tgttttacca
      721 ggtagggtgt cgccaatttg gtgcatatcg ggattgtctg tcaaaacgag ttgtatagct
      781 ttatcgccca acatacctgt ttcataaagc tcagcatacg aacctttggg cactattagg
      841 tgtttatcaa cagatatttc aagggtaatt ggagcatttt ttgtgtaatc aaaatcaata
      901 ttgcttacat gtccaatttt atatccgtta atcaaaacgg gactcgtttt tactacgcca
      961 tctattcggt cgtattgaac gtaaaaatat tttgttggtt taaatagatt tattcctttt
     1021 aggtaattaa taccaaaata cagcagacct agtgctacaa ttactaataa tccaatttta
     1081 gcttctcgtg taaagaattt tttcatagta tatttattat tttttatcag atacttcttt
     1141 tatagcatcg tggatgttac atcttttccc atttttatat gcgattacaa atgcatcagg
     1201 aaattttttt gctacatcgt ttttaagttg gtttatggca tctatatccg atgttgatcc
     1261 tgtggtatat ttataaaaac cattttccac atagctatca atatttgtta ctcctttaaa
     1321 aattgcattt ccctctttgt attttttctt cgacactaaa aactgaactt taaatagaat
     1381 attactttcg gtaacaactg ttgaggtgtt ggtcggtttt tgtgtgggtg tgctggtggt
     1441 agtttgttgg ggtgttggtg tggtatggat ggttgtattt ttaccgtgtt ctatcggttg
     1501 acctccattt tttttctcca atactctttt ataattaaca aatgcttcgt atatagcatc
     1561 taccatttta tcttgatttt cctttctgtt aaggtatttt tcttcttctc tattcgagat
     1621 aaaaccaagt tctaccaata cggaaggcat cgtagtagcc ctaagtacca agaaacctgc
     1681 ctgacgtact cctcggtctg ttaacggagt gttttttgag aaattttgct gtatcaagtc
     1741 tgcaaatttg atgctttgtt ccatatattg gtcttgcata aactgaaaca taatatacga
     1801 atcggttgaa tttgggtcaa atccttgata ggttttttgg taatcttcct ctaatagaat
     1861 aacggagttt tcaagcatag ctaattccat gttttctttg ctacgggcat gaccaagtgt
     1921 aaatgtttcc gtaccattcg ctgttttatt ttttgcagaa ttggtatgaa tagacataaa
     1981 taaatcggca ttggctttgt tcgctatttg tgcacgttca tttaaaggga taaacacatc
     2041 agttgtgcgg gtataaatga tttttacatc tttgtgatta gcagcaattt tttcacccaa
     2101 ggctaaaacg tacgttaagt taatgttttt ttcttttgag aaggagccga tagcaccagc
     2161 atcttgacct ccatgacccg catccagtac taaggtgaag gcaaatgaag atgtgcaaaa
     2221 cagaatgaag gtaaaactag ctatgacttt tttcaatgta ttcatattca ttgggctata
     2281 attaaaatca gtaagaaatc atcaattgtt tgcaaaaata gtggtttttt accgaaactt
     2341 tgggttttta tcgtaaatat ctcctaaaaa aagattaaaa tttattatta tatcctacag
     2401 gtaactttaa aatgtataaa tttgcacaag ttttttttaa gttagcatgc tcactatata
     2461 gatattgtca gttgaagaag ttttgcctct tttttttctt ccttctattc gttggtaaat
     2521 ccgatgtagc actatttgct cagagcaatg aaaatgttgc tatgcctacc gaacgtacga
     2581 gtactcgaat aagctcaaag tctgttatag aggataatat cgtatacaat gccaccgatt
     2641 ctattatcat tttgaaaaat ggaattattc atttacatgg caatgcctct gttacgtatc
     2701 aagatattga attaaaagca gcttatatca gattaaacat agatagtagt ttggtgtttg
     2761 catcgggtat aacagacaca gtaggtactt atcttggtgc tcctattttt aaagaaggtt
     2821 cagaaacgta tgagtcaaaa tcattattgt ataattttaa tactaaaaaa ggattaattt
     2881 ttaatacagt tacacaacaa ggagaaggat atgtaagtag tggtgttacc aaaaagagta
     2941 ctgaaaatgt tttttgtttg cagcatggta aatacactac ctgcgatcac cacgaccatc
     3001 cccattttta cttgtctctt acaaaagcga agatgaaaca gggagaatac attgttacag
     3061 gacccgctta tctggtgata gaagatcttc cgttgcctct ttttgtgcct tttggctatt
     3121 ttccagttcc taaaacgtat tcatcgggag tgcttttccc tacttttgga gacgaacttc
     3181 aacgtggttt ttatgcacgt aacggaggat attattttgc cattaatgat tatgttgatt
     3241 tagctcttac gggcgatgta tacaccaagg gtacctggtc gctaaatgca gcatctagct
     3301 atgtgcttcg ctataaattt agagggaatg tgaatgtaag tttgatggaa aatattgtgg
     3361 gtgaacgtga tatgccagat tatgctaaaa gtaacgattt ccgcattatg tggtcgcatg
     3421 cacaagatgc taaagcaaat ccattttcta ccttatcagc tagtgttaat ttttctacgt
     3481 cttcttacga aagaaacaat ttgaatagtt attataaccc taatttatta tcacaaaata
     3541 cacgtagttc taccattacc tatacgcaac gttttccaga aagtccattt tctttatcag
     3601 caagtgtata tgctaaccaa cgtacacgcg attcaattat cagtttgtcg ttgcctaact
     3661 taaatgtgag tatgagtcgt atttatccat tgaaacgaaa acaagtggtg ggtacagata
     3721 ggtggtacga aaaaattagt atgacctata caggtcgatt ttctaatagt ataactacaa
     3781 aagaagacaa attactcact tcctccttta ccaaagattg gaaaaatggg gtagaacata
     3841 cattacc
//
LOCUS       DAOSMY010000131         3833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_23886_length_3833_cov_3.305188, whole genome shotgun sequence.
ACCESSION   DAOSMY010000131 DAOSMY010000000
VERSION     DAOSMY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23886_length_3833_cov_3.305188"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="PLD76_03970"
     CDS             <1..1400
                     /locus_tag="PLD76_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOU27193.1"
                     /translation="KVRIVQKLHLVPIERRLQALEEGGYNTFQLGTLDVFLDMLTDSG
                     TNAMSDNQLAAMMRADDAYAGSQSFVRLQKAVEEVLGQKYLLPVHQGRAAENILGKAY
                     VKKGSLVPMNYHFTTALAHITENGGSIAELLYPEAYKIDSECLFKGNMNLELLEETIQ
                     KVGVENVPYIRMEASTNLIGGQPFSVQNLRDVKAIAKKYGIRLVLDASLLGENAYLVK
                     QREDEFKQKSMAEVILTMTSLADIIYFSARKLSSSRGGGICTNNEAVYKELEPLVPLF
                     EGFLTYGGISVREVEAMAVGLYETLDETMISQSPEFIKYFVYDAKSKGIPMITPPGVL
                     GAHVDAMSFCSHIPQTQYPAGALAAAFFLISGIRGMERGSVSNQRDENGNETYADMEL
                     LRLAIPRRVFTLSQIKYAIDRLAWLHKNRELIGGLKFVYEPPVLRFFMGKLAPVSDWP
                     QKLIAKFKEDFGDSL"
     gene            1482..2297
                     /locus_tag="PLD76_03975"
     CDS             1482..2297
                     /locus_tag="PLD76_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOU27194.1"
                     /translation="MERMEVLVDKLMSSGFEIGKNVVIALVIFVVGRYLISFVNKLVR
                     AMMERRQLDPAIRSFLGSMINIVLMVLLLISVVGALGVQTTSFAALLASAGIAVGMAL
                     SGNMQNFAGGLMILFFKPYKIGDFIETQNVMGTVKEIQIFHTILATPDNKIIFIPNGP
                     LSNGVLINFSHQLTRRIEWTFGVEYGTDLEKVKQILTSLIEKDSRILLEPTYLIALNK
                     LADSSVNVVVRAWTKNEDYWSVYFEMNQSVYTTFNKEGINFPFPQLVVHHNKE"
     gene            2405..3763
                     /locus_tag="PLD76_03980"
     CDS             2405..3763
                     /locus_tag="PLD76_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU27195.1"
                     /translation="MYLSQYIPFYKKNLKIAIPIILSQLGGGLVTVVDTIMVGHLGTV
                     ELAAASFANSIFLIGFVFSIGLTMAITPLVGKAYAEKKGKEIVELFQNSFLFVIIMAV
                     LIMIILLLAYFFMDYMGQQITVVELAKPYYILLIISLFPFLMFSVYKQFVEGLGNTAV
                     AMYITIIANIINIVFNYLLIFGKFGFPAWGLVGAGIATLLSRIVMWILFVVVVRYNPT
                     WRSYVQSFSWSLFNTKTIKRLASVGFPIGGQMFLEISAFGISTIMVGWLGDVSLAAHQ
                     IAQIMAHVSFMGILGLAAAGTVRVSHEYGHKNIYNLQMSITASRHLVLVMTCFTGIIL
                     LSMRNYIPLIFTTDSTVIEIASTLIVLVGLFQISDGMQAVGAGVLRGLTDVNVMVRYA
                     FFAYICINIPVAYLLAFVVGLREQGIWIGFIFGLSCAAILFHLRYKKKIKLLQTTLNK
                     IS"
BASE COUNT         1179 a          520 c          764 g         1370 t
ORIGIN      
        1 acaaagtgcg tatcgtgcaa aaattacatt tagtacccat tgaacgtcgt ttacaggctc
       61 ttgaagaggg aggatacaat acttttcaat taggaacttt agatgttttt ttagacatgt
      121 tgacagatag tgggacgaat gctatgagcg ataatcagtt agccgcaatg atgcgtgctg
      181 acgatgctta cgcaggctct caaagttttg ttcgtttaca aaaagccgtt gaagaagtat
      241 tgggacaaaa gtatttgtta ccagtgcatc aaggtcgtgc tgccgaaaat attttaggaa
      301 aagcatacgt aaaaaaagga tcattggtgc caatgaatta ccactttacg acagcattag
      361 cacatattac agaaaatggg ggttcaattg ctgagttgct ttatccagaa gcctataaaa
      421 ttgattctga atgtctattt aaaggaaata tgaatttgga attattagaa gaaaccatcc
      481 aaaaagtagg tgttgagaat gtgccttata ttcgtatgga agcttctacc aatttaattg
      541 gggggcaacc tttttctgtg caaaatttgc gagatgtaaa agcaattgca aaaaaatatg
      601 gtatacgatt ggttttagat gcatcattat tgggagagaa tgcttatttg gtaaaacaaa
      661 gagaggatga atttaaacaa aaatcaatgg ctgaggttat tcttactatg acttctttag
      721 cagatattat ttatttctcg gctcgtaagt taagttcatc aagaggagga ggtatttgca
      781 caaataacga agctgtatat aaagagcttg aaccattagt gcctttgttt gaagggttct
      841 taacctatgg cggtatttct gttcgtgaag tagaggctat ggcagtagga ttatacgaaa
      901 cattagacga aacaatgatt tcgcaaagtc ctgaatttat taaatatttt gtgtatgatg
      961 caaagagcaa aggcatacca atgattactc ctcctggagt attaggggct cacgtagatg
     1021 caatgagttt ttgttcacat attcctcaaa cacaataccc tgcgggagct ttagccgctg
     1081 catttttctt aatctctggt attagaggta tggagagagg gtctgtgtct aatcaacgag
     1141 atgaaaatgg caatgaaaca tatgctgata tggaattatt acgtttggcg attcctcgta
     1201 gagtatttac cttgtctcaa ataaaatatg caattgaccg attggcttgg ttacacaaaa
     1261 atagagagtt aattggtgga ttaaaatttg tgtatgaacc accagtttta cgtttcttta
     1321 tggggaaatt agctcctgta tctgattggc ctcaaaaact gattgcaaaa tttaaggaag
     1381 attttggcga tagtttataa gctgtttgtt gtgatatctt gtctactgag gattctttcc
     1441 tcagtagatt ttttttgtat aacaatttta atttttttat tatggaaaga atggaagttt
     1501 tagtagacaa attgatgagt tcaggttttg aaattggaaa aaatgttgtg atagcactag
     1561 tcatttttgt tgttgggcgt tacctaattt catttgtgaa taaattagtg agagccatga
     1621 tggaaagacg tcagttagac ccagccatcc gcagtttttt gggaagtatg attaatattg
     1681 tcttgatggt actattacta atatctgtag taggtgctct tggtgtacaa acaacctctt
     1741 ttgcagcttt attagcatca gcaggtattg ctgtgggtat ggctttgagc ggaaatatgc
     1801 aaaactttgc aggtggattg atgatattat tttttaaacc ttacaaaatt ggcgatttta
     1861 ttgaaaccca aaatgtaatg gggactgtaa aagaaattca gatttttcat actattctag
     1921 caactcctga taataagata atttttattc ctaacggacc tctaagcaat ggagtgctaa
     1981 taaattttag ccatcagtta acacgtagaa ttgaatggac atttggagtg gaatatggta
     2041 ctgaccttga aaaagtgaaa caaatattaa cttctttaat agaaaaagat tcaaggatat
     2101 tgcttgaacc aacatatctt attgcactaa ataaattagc tgatagtagt gtgaatgtgg
     2161 ttgtaagagc ttggactaag aacgaagatt attggtcagt atattttgaa atgaatcaat
     2221 cagtatatac tacatttaat aaagaaggaa ttaattttcc atttccacaa ttggttgttc
     2281 atcacaataa ggagtaaggt gtatttgtta ggaatatgta aaaatagagg gttgtgtttc
     2341 tactctctat ttttttgcac tataatgatt aactttgcgt ttctattaca gaattaataa
     2401 acatatgtat cttagccagt atattccttt ttataagaaa aatttaaaaa ttgcaattcc
     2461 gatcattctt tcacaacttg gaggaggatt ggtaactgta gttgatacca ttatggtggg
     2521 tcatttaggt acagtagaac tagctgcggc ttcatttgca aattctattt ttttaattgg
     2581 ttttgttttt tctattggtt taacaatggc aataacccca ttagtaggaa aagcttatgc
     2641 agaaaaaaag ggaaaagaaa ttgttgaatt gtttcagaat tcttttcttt ttgtcatcat
     2701 tatggctgtt ttaattatga taatactgtt gcttgcctat ttttttatgg attatatggg
     2761 tcaacaaatt actgtagttg aattagcaaa accatattat atcttactaa ttataagttt
     2821 attccctttt ttaatgtttt ctgtttacaa acaatttgta gagggcttag gaaatacagc
     2881 tgttgctatg tatataacca ttatcgccaa tataataaat attgtattta attatctttt
     2941 aatatttggt aaatttggat ttccagcttg gggtttagtg ggagcaggta ttgctacatt
     3001 attatcccgt attgtgatgt ggatactctt tgttgttgtt gtccgatata atcctacttg
     3061 gcgtagttat gtacaatctt tttcatggtc gttgtttaat acaaaaacga ttaaacgtct
     3121 tgcttctgtt gggtttccga ttggtggtca gatgttttta gaaatttccg cctttgggat
     3181 atccactatt atggttggat ggctaggtga tgtttcttta gctgcacatc agattgcaca
     3241 aattatggct catgtttctt ttatggggat attaggatta gctgccgctg gaacagtgcg
     3301 tgttagtcat gagtatggac acaaaaatat ctataatcta caaatgtcaa ttactgcctc
     3361 acgtcatttg gtacttgtta tgacttgttt tacgggaata attttactat caatgcgaaa
     3421 ctatattcca ttaatcttta ccacagattc cactgtaatt gagattgctt ctacattaat
     3481 tgtattagtt ggattgtttc aaatatcaga tggtatgcaa gcagtaggag caggagtttt
     3541 gcgtggacta acagatgtaa atgtaatggt aagatatgct ttttttgctt atatatgtat
     3601 taatattcca gttgcttatc tattggcttt tgtagtagga ctcagagaac aaggtatttg
     3661 gataggtttt atatttggct tgagttgtgc agctatatta tttcatttac gttacaaaaa
     3721 gaagataaaa ttgttacaaa ctaccctaaa taaaatttcg tagttttaag tttttattaa
     3781 atcaacacat tggctaatgt gtttgcaata ttttaggtta cttttgtact att
//
LOCUS       DAOSMY010000132         3806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24116_length_3806_cov_3.526793, whole genome shotgun sequence.
ACCESSION   DAOSMY010000132 DAOSMY010000000
VERSION     DAOSMY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24116_length_3806_cov_3.526793"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLD76_03985"
     CDS             complement(<1..627)
                     /locus_tag="PLD76_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27196.1"
                     /translation="MNWREERHQILAPWSIFLITGVFAIAFWIKSFLSYDMVNFPTEK
                     DILYYYLTQIIPFDSFLAKIIAFLLVVFIAVITFFANNKYFFIKNKSALIIMFMVLLM
                     GLNDPSHNLNPGIIGLTFLLFAFIRFLGMHGKLHTGARSFDIGLYISIGSLFYDRMLF
                     FIPLFYIGFYIYQQLHIKNILATFFGITFPYLLTGGMCFLFDDFDNFVQ"
     gene            complement(624..1898)
                     /gene="purD"
                     /locus_tag="PLD76_03990"
     CDS             complement(624..1898)
                     /gene="purD"
                     /locus_tag="PLD76_03990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOU27197.1"
                     /translation="MKHTILLLGSGGREHAIAWKLTQSTKLNRLFIAPGNAGTAQLGE
                     NISINPEDFVAVQTFVEKNDVTMVIVGPEAPLVAGIVDFFKATPTIAHIPVLGPSKYA
                     AQMEGSKEFAKEFMKRHAIPTAGYLSVTKDNLAQGFAFLETLKAPYVLKADGLAAGKG
                     VLIISDLNEAKTELAAMLDGKFGKASSTVVIEEFLDGIECSVFVLTDGDKYVILPEAK
                     DYKRIGEGDTGPNTGGMGAISPVSFANATFMQQVEERIIRPTINGFRTENIDYKGFVF
                     FGLIKVNNEPYVIEYNARMGDPETEAVMLRLQNDLLTLVEATFDGTLDKQVMKFDTRF
                     AVTVMLVAGGYPGDYQKGDVITGCELVEDSIAFHAGTTLQNNDIVTNGGRVIAISSYG
                     NTKEEALACSFKNAHKINYKGKYFRSDIGFDL"
     gene            complement(2000..2794)
                     /gene="rsmA"
                     /locus_tag="PLD76_03995"
     CDS             complement(2000..2794)
                     /gene="rsmA"
                     /locus_tag="PLD76_03995"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU27198.1"
                     /translation="MSYVKPKKHLGQHFLTDQHVARSIAETLRISEPTKVLEIGPGTG
                     VLTQYLLENELIDLKVIEIDGESVEYLQKEFPALHNRIFFQDFLTTDIPQLYGSETFS
                     VIGNFPYNISSQIFFKILENKHQIPCVCGMLQKEVAERLASKPGKKTYGILSVLLQAY
                     YDVEYLFTVPPTVFNPPPKVNSAVIRVTRNATQQLECNETLFKTVIKTAFNQRRKTMR
                     NSLKGIITVSSPIVTESIFDKRPEQLSVNEFVTLTNLIEPYIQSQH"
     gene            2892..3383
                     /locus_tag="PLD76_04000"
     CDS             2892..3383
                     /locus_tag="PLD76_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU27199.1"
                     /translation="MLVRFGKIIRNYYIQFLDFLYPLFKQWMHKGLFYYLASGGINVG
                     TNWIMYFVVHNFVVQKQIVDLGFIAFSPHIASLIITFPFTFLLGFGLSKYVSFAESTV
                     RTKTQFFRYAGIIAFNFLVSYLSMKLLVEWFGIYPTPSNMITTIITSVVSYFSQKHYS
                     FKK"
     gene            3389..>3806
                     /locus_tag="PLD76_04005"
     CDS             3389..>3806
                     /locus_tag="PLD76_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOU27200.1"
                     /translation="MQFLDKYYKWIKIVIMVCAYGYLAYILLHFDNYEDFTSNFTNLN
                     AQKFIFLLVVFCLLPINLFVESLKWKTLVSVMQKIPIRVAYKAVVAGITTGFFTPNRI
                     GEPLGRIMFLDSQNRSQAIALSYVGAFGQSFATFLCG"
BASE COUNT         1154 a          646 c          683 g         1323 t
ORIGIN      
        1 ttgaacaaaa ttatcaaaat catcaaataa aaaacacata ccgcctgtga gtaaataagg
       61 aaaagtaatt ccaaaaaagg tagctagtat atttttgatg tgtaattgtt ggtaaatata
      121 aaaaccgatg taaaacaagg gaataaaaaa gagcattcta tcataaaata aacttccgat
      181 tgaaatatat aatccaatat caaaagaacg tgctcctgta tgtaatttgc catgcatgcc
      241 caaaaatcgt ataaaggcaa acagaagaaa ggttaagcca ataatacctg ggttaaggtt
      301 gtgcgaaggg tcgtttagtc ccatcaacaa aaccataaac ataatgatta aggctgattt
      361 attttttata aagaaatatt tattgttggc aaaaaacgta attactgcaa tgaaaacaac
      421 cagcaaaaac gcaataattt tggctaaaaa tgaatcaaaa ggaatgattt gagtaagata
      481 ataatatagg atgtcttttt cagtaggaaa atttaccata tcatatgaca ggaaagactt
      541 gatccaaaat gcaatagcaa aaacccccgt aatcaaaaat atggaccacg gtgctagtat
      601 ttgatgtcgc tcttctctcc aattcataaa tcaaatccta tgtctgaacg aaaatatttt
      661 cctttgtaat tgattttatg tgcatttttg aaagagcaag ccaatgcttc ttctttcgta
      721 tttccgtatg aacttattgc tattacacgt cctccgttgg ttacaatatc gttgttttga
      781 agtgtggtgc ctgcatgaaa tgcaatactg tcttcaacaa gttcacaacc tgtaatcaca
      841 tcgccttttt gataatcgcc aggatatcct cctgctacta acattacagt aacagcaaat
      901 cgggtatcaa atttcattac ttgtttatct aaagtgccat caaacgttgc ttcaactagt
      961 gtaagtaaat cattttgtaa gcgtagcatc actgcttctg tctcgggatc acccatacgt
     1021 gcattgtatt ctattacgta aggttcattg tttactttta tcaaaccaaa aaacacaaat
     1081 cctttgtaat caatgttttc tgtgcgaaat ccgtttatag tcggacgaat aattcgttct
     1141 tctacttgtt gcataaaggt agcgttggca aagcttaccg gagaaattgc ccccatacct
     1201 cctgtatttg gacctgtatc gccttcgcct attcgtttgt agtctttggc ttcgggaaga
     1261 ataacatatt tatctccgtc agttagtaca aacacggaac attcgattcc atctaaaaat
     1321 tcttctatca ctactgttga gcttgctttg ccaaatttgc catcaagcat agctgctaat
     1381 tctgtttttg cttcatttaa gtcgctgata atcaaaaccc ctttcccagc tgctaatcca
     1441 tctgctttta acacataggg ggcttttaat gtttccaaaa atgcaaaccc ttgagccaaa
     1501 ttgtcttttg taaccgaaag atatccagct gttggtatag catgtctttt cataaattct
     1561 ttggcaaatt ctttactgcc ttccatttgt gcagcgtatt ttgaaggacc aagaacgggt
     1621 atatgtgcta tcgtaggagt tgctttaaaa aaatctacaa tacctgcaac caaaggagct
     1681 tctggaccaa cgattaccat ggtaacatca tttttttcta caaatgtttg tactgccaca
     1741 aagtcttcgg ggtttatgga gatgttttct ccaagttgag cggtacctgc attgccaggg
     1801 gcgataaata accgattaag ttttgtactt tgagttaatt tccaagcgat ggcgtgttct
     1861 ctgcctcctg atcccaaaag tagtattgtg tgtttcatgt atgtaaaaat ttgcttaatg
     1921 tgttgacgtt acaaaggtac aatttttttc ttttttagat actaattgtt ttcttgtaat
     1981 tagtatgtat acatatgtgt tagtgttgac tttgaatata tggctcaatt aaattagtaa
     2041 gtgtaacaaa ttcgtttaca gaaagttgct ctggacgttt gtcgaatatg ctttctgtta
     2101 caataggaga agatactgtt atgatgcctt ttagcgaatt tcgcattgtt tttcttcgtt
     2161 gattgaaagc tgttttgata acagttttaa ataaggtctc gttgcattct agttgttgag
     2221 tagcgttgcg tgtaacacgt ataactgccg agttgacttt tggcggtggg ttaaacacag
     2281 taggaggtac cgtaaataaa tactccacat cgtagtaggc ttgtaataaa acacttaaaa
     2341 ttccatatgt ctttttccct ggtttcgacg ccaatcgttc cgccacttcc ttttgcaaca
     2401 taccacacac acaggggatt tggtgtttgt tttcaagtat tttaaaaaat atctgactag
     2461 atatattata cgggaaattg cctataacag aaaaagtttc tgatccgtaa agctgaggta
     2521 tatctgttgt caagaaatct tgaaaaaata tgcggttatg caaggcagga aattcttttt
     2581 gtaagtattc aacagactct ccatctatct caattacttt caaatcaatg agttcgtttt
     2641 ccaataaata ctgtgtcaat acgcctgtgc caggacctat ttcgagtact tttgtgggct
     2701 cactaattcg caatgtttcg gcaatgctac gagctacgtg ttggtcggta aggaaatgtt
     2761 gtccaaggtg tttttttggt tttacgtatg acatagtgta tatctttagc aaataataaa
     2821 agcaaaaata tgctttcgtt ttaaaaaagt ttatctttgc aaagataatc aaaaagcaag
     2881 tacctaaaag tatgttagtt cgttttggca aaataatacg caattattac attcaatttc
     2941 tcgatttttt gtatccatta tttaaacagt ggatgcacaa agggctgttt tattatttag
     3001 cttcgggtgg tattaatgtg ggcactaact ggattatgta ttttgtagtg cataactttg
     3061 tggtgcaaaa acagatagtt gacttaggat ttatcgcctt tagtccgcat attgcgagtt
     3121 tgattattac ttttccgttt acctttttac tgggttttgg attatcgaaa tatgtaagtt
     3181 ttgccgaatc gaccgtgcga acgaagacgc aattttttcg atatgctggt ataattgcgt
     3241 ttaacttctt ggtaagttat ctttctatga aattattagt agaatggttt ggtatatatc
     3301 caacgccatc taatatgatt acaactatca taaccagtgt ggttagttat ttttctcaaa
     3361 aacactattc gtttaaaaag taagagagat gcaattttta gataaatatt acaagtggat
     3421 taaaatagtg attatggtgt gtgcctatgg atatttggca tatattttac tccattttga
     3481 taattatgaa gattttactt caaattttac aaacttaaac gctcaaaagt ttatattctt
     3541 gcttgttgtt ttctgcctat tgccaattaa tctatttgta gaatctctga aatggaaaac
     3601 attggtttct gttatgcaaa aaattcctat tcgggtagct tacaaggctg ttgtagctgg
     3661 tatcaccact gggtttttta ccccaaatag aataggagaa cctcttggtc ggattatgtt
     3721 tttggattcg caaaatcgtt cgcaagcaat agcactttcc tacgttggtg cttttggtca
     3781 aagttttgct acgtttttat gtggag
//
LOCUS       DAOSMY010000133         3777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24355_length_3777_cov_4.163084, whole genome shotgun sequence.
ACCESSION   DAOSMY010000133 DAOSMY010000000
VERSION     DAOSMY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24355_length_3777_cov_4.163084"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..>3777)
                     /gene="metH"
                     /locus_tag="PLD76_04010"
     CDS             complement(200..>3777)
                     /gene="metH"
                     /locus_tag="PLD76_04010"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOU27201.1"
                     /translation="FTEADIDRLIEDDLPAGDMTTFLLDLAGKKGSIALIARNEMIAC
                     CTEEAVRLYKKAGLEVGDFVASGTRLQPGDRLIEAKGEASAIHIIWRTGSAMIEFASG
                     IAGRTNQLVMAAKKENPSISVAGTRKHPPFVKKMALKALMAGGVDVLLVETIFDTLNA
                     KAAIYAIQDVFARLGKEIPIIISGTLLDKSARTLSGQTLEAFVISVSHAKPLAIGLNC
                     STGASDMLPHIIELGKLTDTFVSAYPNAGFPNELGKYDETPQQMSNQIEEFLSHQAVN
                     IIGGCCGTTPEHITRFAQLAASATPHQRTTLEPFTRLSGLEPMVIKKENNFVNIGERT
                     NVAGSKKFARLIADKKYEEAIQIARQQVEGGAQIIDICMDDAMLDSAASMTDFLQLVA
                     TEPSVAKVPIMIDSSKWEVLEAGLRCVQGKSVINSISLKEGEEVFLNHAKKIRQYGAA
                     VVVMAFDEQGQADTFEKRIAVCQRAYTLLTNIGFPAEDIIFDPNILAIATGLEEHANY
                     AIDFIRTVEWIKKELPYAKVSGGVSNLSFSFRGNNTVREAMHSVFLYYATQKGMDMGI
                     VNPEMLEIYSNIPTDLLELTEDVIFNRRADATERLINYANEVKSNTSKETKTDEWRTK
                     SLQERLGHAIIKGIYDYLDTDLTEARTQYAKALEIIEGPLMDGMNIVGDLFGSGKMFL
                     PQVVKSAQVMKKAVAFLLPYIEAEKDSNSQNAGKIVMATVKGDVHDIGKNIVGVVLAC
                     NNFEIIDLGVMVPASEILRKAKEVNADIIGLSGLITPSLEEMASVAEEMEKQGFTLPL
                     LVGGATTSKLHTSVKIAPCYRHGVVHVIDASKTIPVAKALCDRNKKEAFLAEIDIQHR
                     ETREKYASLKKVELISLAEARNNALQIDWKKEQIHVPKITGLQIWKNYPIEELIPYID
                     WTYFFVAWQLKEKYPKILEDSPFKEEAIKLFADAQKILERMATEKLVVANGVFGIYPA
                     NTVEDTVEVYADENREKPIALFHFLRQQTKKKNDEKNLSLADYIPSKKSGIGGYIGGF
                     AITAGIHLTELTDSYEKEGDDYSAIMCKILSDRLAEAFAEKLHADIRTTYWGYETIHA
                     TPQEMFEGKYQGIRPAIGYPSIPDHSEKETLFNLLQATEHTGITLTENYAMFPNASVC
                     GLYFDTARASYFNIGRIGEDQVNDYANRKQIDITFLKKLIAKNLE"
BASE COUNT          980 a          782 c          726 g         1289 t
ORIGIN      
        1 actgtggtag gtcatattga tatacgaagg attgaatgct aaaagagggt ctatcgcttt
       61 gtatatggca tcaattcctt ttcctttaag gggaggtaat agttcaaaag taaaaagggg
      121 cgatgttgct tgtttgagtt tatctgctac gttcatgttg tattttttcg ttaagccaac
      181 aaaattacga aaacttattt tattctaaat tttttgctat gagttttttt aaaaaagtga
      241 tatcaatttg tttccgattg gcgtaatcgt ttacttggtc ttctccaatt cgtcctatat
      301 tgaagtacga tgctctggca gtatcaaaat acaaaccgca aacacttgca ttcggaaaca
      361 tggcgtaatt ctctgtcaag gtaatgcctg tatgttcagt tgcttgtagc aagttgaata
      421 aggtttcttt ttcagagtgg tcggggattg atgggtagcc gatggcgggt cgaatgcctt
      481 ggtacttgcc ttcaaacatt tcttgtggtg ttgcgtgtat tgtttcgtaa ccccagtagg
      541 ttgtacgtat atcagcatgt aatttttcgg caaatgcttc ggctaatcta tcgctcaata
      601 ttttgcacat aatggcagaa tagtcgtcgc cttctttttc gtaactatct gttaattctg
      661 taagatgtat gcccgcagta atagcaaaac cgccaatata accgcctatc cctgatttct
      721 tagaaggaat ataatcggca agcgaaaggt ttttttcgtc gttcttcttc tttgtttgtt
      781 gacgcaagaa atgaaacaat gcgattggtt tttcgcgatt ctcatcagca tatacttcta
      841 cggtatcctc taccgtattg gctggataaa tgccaaatac tccattggcg actaccaatt
      901 tttcagttgc catacgctca agtatctttt gggcatcagc aaatagctta atagcttctt
      961 ctttaaaagg gctatcttct agtattttcg ggtatttttc ttttaactgc catgccacga
     1021 aaaagtatgt ccaatcaata tatggaataa gctcttcaat tgggtaattt ttccaaattt
     1081 gcaagcctgt tattttggga acatgaatct gttctttttt ccaatctatt tgtagggcat
     1141 tgttacgtgc ttcagccaat gaaataagtt caactttttt caacgaggca tatttctctc
     1201 tggtttcacg gtgttgtata tcaatttcag ctaagaaagc ttcttttttg ttcctgtcgc
     1261 acaatgcttt agcaactgga atggtttttg atgcgtctat tacatgaact acaccgtgtc
     1321 tataacaagg agctattttc acgcttgtat gcaacttgga ggtagtagct cctccaacga
     1381 gcagaggcaa ggtaaaacct tgtttttcca tttcttctgc taccgatgcc atttcttcga
     1441 gtgagggggt tattaagcca ctcaatccga tgatatcggc gtttacttct ttggctttgc
     1501 gtaatatttc tgatgcaggc accattacac caaggtcgat aatttcaaaa ttattgcagg
     1561 caagtactac tcctacaatg ttcttaccaa tatcatgaac atcccctttt actgtagcca
     1621 tcacaatttt gccagcattc tgactgttgc tgtctttttc tgcctcgata taggggagta
     1681 aaaaggcaac ggcttttttc attacttgtg cactttttac cacttgtggc agaaacattt
     1741 ttcccgaacc aaacaaatca cccacaatat tcattccgtc catgagcgga ccttcgatta
     1801 tttctaatgc tttggcatat tgtgtacgag cttctgtgag gtcggtatct aaataatcgt
     1861 aaatgccttt aataatagcg tgtcctaaac gttcttgtaa tgacttggta cgccattcat
     1921 ctgtttttgt ttctttgctg gtgttacttt taacttcgtt ggcatagttt atcaatcgtt
     1981 ctgtagcatc ggctctgcga ttaaaaatca catcttctgt tagttcaagc aagtcggttg
     2041 gtatgttgct gtatatttcg agcatttctg gatttactat tcccatatcc ataccttttt
     2101 gcgtagcata gtataaaaat accgaatgca tggcttctcg caccgtattg ttccctctaa
     2161 acgagaagga taggttgctt actccaccac ttactttggc ataaggaagt tcttttttta
     2221 tccattctac ggtacgaata aaatcaattg cgtaattagc atgttcctca agtccagtag
     2281 caatagctaa gatattggga tcaaaaataa tgtcttcggc aggaaaacct atgttagtca
     2341 atagtgtata ggcacgttgg catacagcta tgcgtttttc aaatgtatcg gcttgacctt
     2401 gttcgtcaaa tgccatcact accacggctg ctccatattg acgaattttt tttgcgtgat
     2461 taagaaatac ttcttctccc tcttttagcg aaatagaatt aataaccgat ttgccttgta
     2521 cgcatctaag accagcttct aatacctccc attttgatga atcaatcata attggcactt
     2581 tggctactga gggttctgtg gcaactaatt gtagaaaatc agtcatacta gcagcggagt
     2641 cgagcatagc atcgtccatg caaatatcaa taatttgagc tccaccttct acttgctgac
     2701 gagcaatttg tatggcttct tcgtattttt tatcggctat taagcgtgca aatttttttg
     2761 accctgccac gttagtacgt tcgccaatgt ttacaaaatt attctctttt ttgattacca
     2821 ttggttctaa tccactcaaa cgagtgaatg gttccaaagt agtacgttgg tgaggtgtag
     2881 cactagctgc aagttgtgca aaacgggtaa tgtgttcggg agtagtacca caacatcctc
     2941 caataatatt aaccgcttga tgggataaaa actcttcaat ttggttagac atttgctgag
     3001 gtgtttcatc atacttaccc agttcgttcg ggaaacctgc attgggatat gcactcacaa
     3061 acgtatctgt cagttttcct aattcaatga tatgaggcaa catatcagat gctccagtcg
     3121 aacagtttaa gccaatagcc aaaggtttag cgtgagacac agatattaca aaagcttcga
     3181 gcgtttgtcc actcagcgta cgggcacttt tatcgagtaa agtacctgaa ataatgatgg
     3241 gaatttcttt gcccaagcgt gcaaatacat cttgtatggc atagatggct gcttttgcat
     3301 tgagcgtgtc gaaaattgtt tcgactaaca acacatctac tcctcctgcc atcaatgctt
     3361 ttaatgccat ttttttaaca aacggggggt gtttccgggt ccctgccact gatattgaag
     3421 gattttcttt ctttgctgcc attaccagtt gatttgtccg acctgcaata cctgaagcaa
     3481 actcgatcat cgcacttccc gtccgccaga taatatggat tgcagaggcc tctcctttcg
     3541 cttcaatcag tctgtctcca ggttgtaatc tggtacctga tgcgacaaaa tctccaacct
     3601 ccagtcctgc ttttttataa agccggactg cttcttcagt acagcaggct atcatctcat
     3661 ttcgggcgat caatgcaata gatcctttct ttcctgcgag atctaacagg aaggttgtca
     3721 tatctccagc cggtagatcg tcttctatca gtcggtcaat atctgcttca gtaaaat
//
LOCUS       DAOSMY010000134         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24458_length_3765_cov_4.254447, whole genome shotgun sequence.
ACCESSION   DAOSMY010000134 DAOSMY010000000
VERSION     DAOSMY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24458_length_3765_cov_4.254447"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1259)
                     /locus_tag="PLD76_04015"
     CDS             complement(63..1259)
                     /locus_tag="PLD76_04015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU27202.1"
                     /translation="MSAVSPRLASLSESETLAMSQKSNELKAKGFDVINLSVGEPDFP
                     TPTHIKDAAKKAVDNNFSFYSPVPGYPALRNAIVEKLKKENGLQYTADQIVCSNGAKQ
                     SVCNVLLSIIGKGDEVIVPAPYWVSYVEMVKLAEGTNVIVSAGIEQDFKITPDQLEAA
                     ITPKTKALILCSPSNPTGSVYSKAELGALAEVIARYPSIIVLSDEIYEHINYVGKHES
                     IAQFDAIRDRVVIINGVSKGYAMTGWRIGWIAAPKWIAAACNKLQGQYTSGPCSVAQK
                     AAEEAYIGNQECVADMRKAFERRKNLVFSLAKEIEGLQVNNPEGAFYVFPKCDSFFGK
                     KTPKGNVIADAGDLAMYLLEEAHVACVGGAAFGAPDCIRFSYAASDENLVKAFDRIKT
                     ALAELV"
     gene            1413..2300
                     /gene="era"
                     /locus_tag="PLD76_04020"
     CDS             1413..2300
                     /gene="era"
                     /locus_tag="PLD76_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU27203.1"
                     /translation="MHKAGFVNIVGNPNVGKSTLMNALVGEKISIITSKSQTTRHRIM
                     GIVNGDDFQIVYSDTPGVLKPNYRLQESMLAFSQSALIDADVLLYVTDTVEMHDKNVD
                     FLNKIKGVDVPIFVIINKIDLLPNQEKLEELVALWQNTLPKAHIIPISATEKFNVDYV
                     LKQIKDLLPESPPYFEKDALTDRPARFFVTEIIREKILTNYDKEIPYSVEVVIEKFKE
                     TTKQIDISALIYVERDSQKGIIIGHGGKSIKKMGTEARLDIEKFFDKKVFIEFFVKVE
                     KDWRNKDKQLKDFGYYLNS"
     gene            2331..3647
                     /gene="der"
                     /locus_tag="PLD76_04025"
     CDS             2331..3647
                     /gene="der"
                     /locus_tag="PLD76_04025"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU27204.1"
                     /translation="MGSIVAIIGRPNVGKSTLFNRLTESRQAIVNEVAGTTRDRQYGK
                     SEWGGREFSVIDTGGWTFKSEDTFEEEIKKQVDIAIAEADVLLFVVDGQNGVTDLDME
                     VAQLLRKCKKPIVLAVNKTDNNELQYMAADFYKLGIGEPLCISAISGFGSGDLLDVIL
                     PLLPKKEDAPVYDEELPRIAIVGRPNAGKSSIINAFIGEERTIVTDIAGTTRDSIYSR
                     YNKFGKDFYLVDTAGIRKKGKVHEDLEYYSVIRSIKAIENSDVCVLMIDASRGVESQD
                     LNIFSIIQKNRKSLVVCVNKWDLVENKETNTSKEFEQTIRDRFAPFVDFPIIFASALT
                     KQRIFKVLETATEVYENKTQHISTSVLNDFFLPLIENYPPPALKGKYVKIKYVTQIRE
                     AKTPTFIFFANLPQYVKDPYKRFLENKIRANWNFHGNPIQVFIRKK"
BASE COUNT         1252 a          699 c          668 g         1146 t
ORIGIN      
        1 gttcattgtt aatgtttcaa tgctgttgct gggttttctt ccactgaatg ctaacagtaa
       61 tattacacta actcagctaa ggctgttttg atacgatcaa aagcttttac taaattttca
      121 tccgacgctg catacgaaaa acgaatacag tctggagctc caaaagcggc accacccaca
      181 caggcaacgt gagcctcttc caataaatac atcgctaaat ctcccgcatc agcaataaca
      241 tttcccttgg gtgttttttt tccaaaaaaa gaatcacatt taggaaaaac ataaaatgcc
      301 ccttctgggt tgtttacttg taagccttct atttcttttg ctaaagagaa tactaaattc
      361 tttcttcgtt caaaggcttt acgcatatct gctacgcatt cttgattacc aatatatgct
      421 tcttctgcag ctttttgggc taccgaacag ggtccagaag tatattgtcc ttgcagttta
      481 ttgcatgctg ctgctatcca tttaggtgct gctatccaac caatacgcca gccagtcata
      541 gcataccctt tagatactcc gttaataata acaaccctat cgcgtattgc atcaaattgg
      601 gctatgcttt cgtgtttgcc aacatagttg atatgctcat aaatctcgtc tgacaataca
      661 attattgaag gatatcgtgc tataacctca gccaaagcac ctaattctgc tttagaatac
      721 acactgcctg taggattaga tggagaacac aatatcaagg ctttggtttt gggtgttata
      781 gccgcctcta attgatctgg agtaatttta aagtcttgtt caattccagc agaaacaatc
      841 acgtttgtgc cttctgccaa ttttaccatc tctacataac ttacccagta aggagctggc
      901 acaattacct catctccctt accaataata ctcaacaata cattacaaac agattgtttg
      961 gctccattgg agcacacaat ttgatctgcc gtatactgta aaccattttc cttttttaat
     1021 ttttctacaa tagcatttcg caaagctggg tatccaggca ctggcgaata aaacgagaaa
     1081 ttgttatcta ctgccttttt agcagcatct ttgatatgtg taggcgttgg aaaatcgggc
     1141 tctcctacac ttaaattaat tacatcaaac cctttggctt ttaattcatt gcttttttga
     1201 gacatggcta gcgtttccga ttcggacaaa cttgctaatc gtggagatac agcactcatt
     1261 tttctgattt attgtgattt atacttgcgt tttttacttt gcgaacgaac acaaaatagc
     1321 ccacaaaaat acataattgt tatacaacat acaaggaatt tatcggtttc cgaagaaaaa
     1381 aacgtatctt tgcagtcata atttcaactt ttatgcataa agcaggtttt gtaaatattg
     1441 taggtaaccc caatgttggg aaatcaaccc ttatgaatgc cttagtcggt gaaaagattt
     1501 cgattataac atcgaaatca caaactacgc gtcatcgcat tatgggtatt gtaaatggag
     1561 atgactttca gattgtatat tctgatacac ctggcgtgtt aaaaccaaac tatcgactac
     1621 aagaatctat gttggctttc tcccaatcgg ctttgattga cgctgatgtg cttttgtacg
     1681 ttaccgacac agtagaaatg cacgataaaa acgtggattt tctcaacaaa attaaaggtg
     1741 tagatgtgcc aatctttgtt attattaata agatagactt gttgccaaac caagaaaaac
     1801 tagaagaact ggttgcttta tggcaaaaca cattacccaa agcacacata atccccatct
     1861 cggcaacgga aaaatttaat gtggattacg tgctaaaaca aattaaagat ttattgccag
     1921 agtcacctcc ttattttgaa aaagatgcct taaccgaccg acctgcacgg ttttttgtaa
     1981 ccgaaattat acgagaaaaa atcttaacca attacgacaa agaaatacct tattcggtag
     2041 aagttgtaat tgagaaattt aaagaaacca caaaacaaat cgacatcagt gctttgatat
     2101 acgtagaaag agattcgcaa aaaggcataa ttattgggca tggaggaaag tcgataaaaa
     2161 aaatgggcac agaggctcgt ttagatattg aaaagttttt tgacaaaaaa gtatttattg
     2221 aattctttgt taaagtagaa aaagactggc gaaacaaaga taaacagcta aaagattttg
     2281 gttattacct caattcttag tcagcaacat tattaattta taaaaataaa atgggaagta
     2341 tagtagccat tattgggcga ccaaatgtag gaaaatcaac tttattcaat cgtttaacag
     2401 aaagccgtca agctatcgta aatgaggttg cgggcactac gcgcgaccgc cagtacggaa
     2461 aatcagaatg gggaggacgc gaattttctg taatcgatac aggaggatgg acatttaaat
     2521 cggaagacac gtttgaagaa gaaattaaaa aacaagttga tattgctatt gccgaagcag
     2581 atgtgttgtt gtttgtagta gatggacaga atggggttac agaccttgat atggaagtag
     2641 cacaattgct ccgtaaatgc aaaaaaccaa ttgttttagc tgttaacaaa acagacaata
     2701 atgaattaca atatatggct gctgatttct acaagctagg tattggagaa cctctttgta
     2761 tctctgctat cagtggtttt ggatcgggcg atttactgga tgttattttg cctttattgc
     2821 caaaaaaaga agatgctcct gtgtatgatg aagaattgcc tcgtattgcc attgtaggac
     2881 gccccaatgc aggaaaatcg tcgattatca atgcttttat tggcgaagaa agaacaattg
     2941 taaccgatat tgcaggcact acacgcgatt cgatatactc gagatacaac aagtttggaa
     3001 aagattttta tttagtagat actgctggca tacgaaaaaa aggaaaagtt cacgaagatt
     3061 tggaatatta ttcggttatt cgctctatta aagccatcga aaactcagat gtatgtgtat
     3121 taatgattga tgcttctaga ggcgtagaaa gtcaggactt aaatatattc tccatcatac
     3181 aaaaaaaccg caaaagcttg gttgtatgtg tcaacaaatg ggatttagtt gaaaacaagg
     3241 aaacgaatac gagcaaagaa tttgaacaaa ccattcggga tagatttgcc ccttttgtgg
     3301 atttccctat tatttttgca tcagccctta ccaaacaacg gatttttaaa gtgcttgaaa
     3361 cagctaccga agtatatgag aataaaacac agcatatttc aaccagtgtg ctcaatgatt
     3421 ttttcttacc tctaatcgaa aattatccac cgccagctct taaaggtaaa tatgtgaaaa
     3481 ttaaatatgt aacacaaata cgtgaagcaa aaacacctac gtttatcttt tttgcaaatt
     3541 taccgcaata cgtaaaagac ccttacaaac gttttttaga aaataaaatc agagctaatt
     3601 ggaattttca tggaaaccca attcaagtat ttatcagaaa gaagtaagcc atacggcttg
     3661 cttctttctt tatatgtgaa tacgtcagca taaaatggat attctttaac caaactataa
     3721 tcaatcctta ccagatataa tatcttcagt taataaacta ttgta
//
LOCUS       DAOSMY010000135         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24516_length_3757_cov_4.024851, whole genome shotgun sequence.
ACCESSION   DAOSMY010000135 DAOSMY010000000
VERSION     DAOSMY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24516_length_3757_cov_4.024851"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLD76_04030"
     CDS             <1..758
                     /locus_tag="PLD76_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU27205.1"
                     /translation="AQNKAQEIEKVKADLENKLAEIEKARLERVAVLNDRRKENAKLQ
                     TEQRKQSNFVSQLSRKEKDLRRELKKQQDQANAINRRIDQIIAEEARKSQKAQAAGTQ
                     EFALTKEQKLLSGGFEKNQGRLPWPVERGVIVGRFGVQAHAVLKYVTTDNKGIYIQTA
                     PNSDARAVYDGEVTQRFSIPGSNYTVIVRHGNYRTVYSNLTDIYVKVGDNIKARQAIG
                     RIFVDNEDDNKSVLYFQVWKERDIQNPENWITR"
     gene            1286..2053
                     /locus_tag="PLD76_04035"
     CDS             1286..2053
                     /locus_tag="PLD76_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27206.1"
                     /translation="MKQYLEELHKLRIFRLKDVETLVGNLNSAKDLLQNYKKQRLICQ
                     VRRNLYVVTDLASKVSLANKFEIAGKISPTSYLSYQSALEFHGLAHQVFYDLWVSSSK
                     RFNSFEFEGIRFQYCESKSLSGVMNANSSPFIRVTNLERTVIDCIDKIERCGGLEELI
                     QSISLISYLDQELLLNYLQEFNKQFLYQKVGFILEYFKQEMKLSDNFFSICKHKSGKS
                     TRYLTDRIESIQYFKEWKLCAPTNILSVLEQGGENYV"
     gene            2046..2990
                     /locus_tag="PLD76_04040"
     CDS             2046..2990
                     /locus_tag="PLD76_04040"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOU27207.1"
                     /translation="MYKYNKNDLDLLAVNTDFLRDNLEKVLRLSDILQYMNANPLLFS
                     HLALKGGTAINLVVFNLPRLSVDIDLDFTKHCLRDEMFVLRKQINSDILKYMTSQGYQ
                     LNPSSKNPHSLDSWVFYYLNSSGNKDNIKIEINYSMRNHVFEPIEKEVYMESLSLHLH
                     VRTLTNLELFGSKIKALIERTAARDLYDVYNMLMLPVFSVSEQSLLRKIVILYLAIGG
                     NTPPKTDFNFKAIDNLNFSQIKRKLIPVLKKSDKFEFEVAKSKVKTYLTTLMVLTDNE
                     KLFLERFNQKIYQPELLFDELDIVGRINDHPMAIWKCS"
     gene            3153..>3757
                     /locus_tag="PLD76_04045"
     CDS             3153..>3757
                     /locus_tag="PLD76_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU27208.1"
                     /translation="MPKTFFSKYKIPTKLHIPVTSFLFKWLFFALLVGVLAGSAAALL
                     LVSLDFVTNIRESNIGLIGFLPLAGLGIGLMYHYLGSSVVKGNNQILEQIQSPTQTIP
                     FRMAPLIFIGTVLTHLFGGSAGREGTAVQMGAAISDQFAKWFKLTSNDKRILLIIGIS
                     AGFAGVFGTPLAGAIFALEVVVIGRMRYEAILPAFLAAAIA"
BASE COUNT         1224 a          576 c          754 g         1203 t
ORIGIN      
        1 ttgctcaaaa taaagcccaa gaaatagaga aagtaaaagc agatttagaa aacaaattag
       61 cagaaataga aaaagcccgt ttagagcgtg tggctgtatt aaatgataga cgaaaggaga
      121 atgctaaatt gcaaaccgaa caacgtaagc aaagcaattt tgtttcgcaa ttaagtagaa
      181 aagaaaaaga tttgcgtaga gaacttaaaa aacaacaaga tcaagccaat gccattaatc
      241 gtcgtataga tcagattatt gcagaagaag cccgcaaatc acaaaaagca caagccgcag
      301 gcacacaaga atttgcttta acgaaagaac agaaactgct ttctggaggc tttgaaaaga
      361 accaaggtcg cctaccatgg cccgttgaac gaggcgtaat tgtagggcgt tttggcgtgc
      421 aagctcatgc tgtgctaaag tatgtaacaa ccgataataa aggtatttat attcaaactg
      481 ctccaaattc ggatgcacgt gctgtatatg acggggaagt aacccaacgt ttctccattc
      541 caggaagcaa ttatacggtg attgtacgcc acggtaacta ccgtacggta tattctaact
      601 taactgatat atacgtaaag gtaggagata atatcaaggc tcgtcaagcc attgggcgta
      661 tatttgtcga caacgaagac gacaacaagt ccgtactcta ttttcaagta tggaaagaac
      721 gcgatatcca aaaccccgaa aactggatta ctaggtaaaa aaggggattg tttgagggtg
      781 gttaatgctt taaatcgaag ttgattataa agacaattac agatacagct tgcagatttg
      841 ctacagcgat gaggattgaa tcaaataaat aatccgttgg ataaattata tttaggataa
      901 gacaaatacc ttactcaata tgattacttt tgcaacatag ttttaccaca gattgtatgc
      961 ctaaaacatt ttttcaaaat acaaaattcc cagttgagcc tagtgtggta aaaacagttt
     1021 gttggtttag cgtagtttac aaagctacct cattactaaa agcaaagtgc tagcttagca
     1081 aaaggcaaga tccttctttt tttgaaaaga ccaaggcaga gacattcgct taaaataaat
     1141 aggttacgga gaaaggggaa ttattaatgt ctgtttatta attgtgaata tactttatat
     1201 tgctagaaaa atcaaaaaat ctttatattc attgtaatag tgtggttttt tcagtatctt
     1261 tgcgtttatt ataaagtaaa tgccgatgaa acagtattta gaggagctac ataaactacg
     1321 gatattccgc ttgaaagatg ttgaaactct tgtggggaat ttgaattcag ccaaagattt
     1381 attgcaaaac tacaaaaagc aacgattgat ttgtcaggta cgacgaaact tgtatgttgt
     1441 aactgatttg gcaagtaaag ttagcttggc aaataagttt gagattgcag gcaaaatatc
     1501 cccaacatcg tatctttcat accaatcggc attggagttt catggactgg cacatcaggt
     1561 attttacgat ttgtgggttt cgagtagtaa gcgctttaat tcgttcgagt ttgaaggtat
     1621 tcgatttcag tattgcgaat cgaaaagttt gtcgggagta atgaatgcta actccagtcc
     1681 atttattcga gttactaatt tagaacgtac tgtgattgat tgtattgata aaatagagcg
     1741 atgtggggga ttggaagaat taattcagtc tatttcactt atttcctatt tggatcagga
     1801 attactgctc aattacttgc aagaattcaa caagcagttt ttatatcaga aggtaggatt
     1861 tattttggag tattttaagc aagaaatgaa gcttagtgat aactttttta gcatttgcaa
     1921 gcataagtca ggtaaaagca cacgctattt gacagatcgt attgaatcta ttcaatattt
     1981 caaagaatgg aaattgtgtg ctccaactaa tattctttcc gttttagaac aaggaggtga
     2041 aaactatgta taaatacaac aaaaatgatt tagatttgct tgctgtcaat actgattttc
     2101 tccgcgataa tttagagaag gtgcttcgat tgagtgatat cttgcaatac atgaatgcca
     2161 atcctttgct attcagtcat ctggcattaa aaggaggtac agccattaat ctagtggttt
     2221 ttaatcttcc aaggttgtcg gtcgatattg atctggattt tacaaagcat tgtttacgtg
     2281 acgaaatgtt tgtgttacgt aaacaaatta acagtgatat tttaaaatac atgacatcgc
     2341 aagggtatca actaaatcca tcttctaaaa atccgcattc gctggattca tgggtttttt
     2401 actacctaaa ttctagtgga aacaaagata atatcaaaat tgagattaac tattcaatgc
     2461 gaaaccatgt ttttgaaccc attgaaaaag aggtgtatat ggaatcctta tctttacatc
     2521 ttcacgtaag aacgttgact aatttagagc tatttggcag taaaataaaa gcacttattg
     2581 agcgaacagc tgcaagagat ttatatgatg tatataatat gcttatgctt cctgttttct
     2641 cagtttcaga acaatcatta cttcgtaaaa tagtgatttt atatttggca attggtggca
     2701 atacacctcc taaaaccgac tttaacttta aggctattga taatctgaat tttagtcaga
     2761 taaagcgtaa attaatacca gtgttaaaga aaagtgataa gtttgaattt gaggtggcaa
     2821 aatcgaaggt gaaaacttat ctaacaactt taatggtgct tactgataat gagaagctat
     2881 ttcttgaaag attcaatcag aaaatatatc agccagaatt actttttgac gaacttgaca
     2941 ttgtgggtag aattaacgat catccaatgg caatttggaa atgcagttga ggtaataatc
     3001 tgtataattg ctttaaaaaa catatttaag ttgcagtttg ctagcatgtt attataggag
     3061 ttattagttc gaggtaagaa ataaatcgtt atttaatcag tctcttttag tttttatgat
     3121 tacttttgca acatagtttt acaacagatt gtatgcctaa aacatttttt tcgaaatata
     3181 aaatcccaac aaaactgcat atccctgtaa caagcttttt gtttaagtgg ttattttttg
     3241 ctttgttggt gggagttttg gcgggttcgg cggctgcttt gttgttggta tcgttagatt
     3301 ttgtaaccaa tattagagaa agtaatattg ggctaattgg gttcttacct ttggcgggtt
     3361 tgggtatcgg gctgatgtat cattatttag gttcatcggt tgtaaaaggc aataaccaaa
     3421 tactagagca aatacaatca cctacgcaaa ctatcccttt tcgtatggca ccacttattt
     3481 ttattggtac ggtgctcaca catttgttcg gaggttctgc tggtcgagag ggcactgctg
     3541 tgcaaatggg agcagccata tccgatcaat ttgctaagtg gtttaagcta acttctaacg
     3601 ataaacgcat tttgcttatt attggtatta gtgctggttt tgctggggtg tttggtactc
     3661 ccttggctgg agctattttt gctttggaag ttgtggtaat aggtcgtatg cgttacgaag
     3721 ctattttgcc tgcttttttg gcggctgcta ttgctca
//
LOCUS       DAOSMY010000136         3745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24621_length_3745_cov_3.211924, whole genome shotgun sequence.
ACCESSION   DAOSMY010000136 DAOSMY010000000
VERSION     DAOSMY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24621_length_3745_cov_3.211924"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1184)
                     /gene="gmd"
                     /locus_tag="PLD76_04050"
     CDS             complement(132..1184)
                     /gene="gmd"
                     /locus_tag="PLD76_04050"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU27209.1"
                     /translation="MKVALITGITGQDGSYLAEFLLEKGYEVHGVLRRSSSFNTERIE
                     HLYLTEWVRDMKKKRAINLHYGDMTDSSSLVRIIQDVKPDEIYNLAAQSHVKVSFDVP
                     EYTAECDAVGTLRLLEAVRILNLEKECKIYQASTSELFGLVQEIPQKETTPFYPRSPY
                     GVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGETFVTRKITLAAARIAQGMQDK
                     LYMGNMDSLRDWGYAKDYVQCMWMILQHPTPEDFVIATGEMHTVREFCTLAFAEAGIE
                     LRWEGTGVDEKGIDVKTGKALVEVDPKYFRPAEVELLLGDPTKAKTLLGWNPTQTSFE
                     ELVKIMVRHDMEYVRR"
     gene            complement(1197..2312)
                     /gene="rffA"
                     /locus_tag="PLD76_04055"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1197..2312)
                     /gene="rffA"
                     /locus_tag="PLD76_04055"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOU27210.1"
                     /translation="MIPFNKPYLTGKETHYIYEAVNSGKLSGNGIFTKRCQDYFEQRY
                     GFKKCLLTTSCTDALEMAAILCNIQPGDEVIVPSYTFVSSALAFVRAGATIVFADSMA
                     DNPNIDANKLEDLITAKTKVIVPVHYAGVACDMDAIMEIANKHSIWVVEDAAQAVDSY
                     YKGKPLGSIGHLSAFSFHETKNIIAGEGGMLAINDERFIHRAEIIWEKGTNRAEFFRG
                     EVNKYGWVDTGSSFLPSEVIAAFLWAQIENLDDIQRRRKEIWQLYYDNLVSLPAIKGI
                     GLPIIPNYATNNAHMFYLVCNNLEQRTELIKKLKKNDILAVFHYLSLHSSDYYANKHD
                     GRTLPNGDRFADCLVRLPLYYELESNKIIEICRLITN"
     gene            complement(2325..3542)
                     /locus_tag="PLD76_04060"
     CDS             complement(2325..3542)
                     /locus_tag="PLD76_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU27211.1"
                     /translation="MIARDQYLDKILSFKDQPLIKIITGIRRCGKSTVLSLLKKYLLS
                     NGVSAKQIIAINFESFAFSEFTDAQKLYLFISKQIIPQNKYYLLIDEVQEVTNWEKCI
                     NSLLVDFDIDIYITGSNSHLLSSELATHLAGRYVEIPIYTLSFSEYLSFNAHYKTNIS
                     EDTRKQFINYLRQGGFPIVHTSSYSEESMYKIIFDIYSSIILRDTIQRYKIRDVELLE
                     RVVKYVFSNIGNTFSGKNVADYFKSQFRKIDINTVYNYLYALEGAFIIHRVSRYDIKG
                     KELLKTQEKFYLSDISLLYATMGYKDTLISGILENIVFLELKRRGYNVFVGKLDSKEI
                     DFIAEKADKKIYIQVAYKLENPETIEREFSVLLSINDQYPKYVVTMDDFWNESIQGIH
                     HCHIADFLLDNSY"
BASE COUNT         1183 a          729 c          550 g         1283 t
ORIGIN      
        1 aaacgagcat cattataaac ttctaacttt tcaaatccgt atattttctt atcaaacata
       61 atatctttaa tttatcactt aacgttaaac gttaaacact aaacgttaaa cattaaacgc
      121 taaacgctta actatcttct cacatattcc atgtcatgac gcaccatgat ttttaccaat
      181 tcttcaaaag atgtttgagt tgggttccaa cctaacaatg tttttgcttt tgttggatct
      241 cctagtaata attctacttc agcaggacgg aaatattttg gatcaacctc aactaatgct
      301 tttccagttt ttacatcaat acctttttca tctacacccg tgccttccca acgcaattcg
      361 atacctgctt cggcaaaagc tagggtacaa aattcacgta cggtatgcat ttctcctgta
      421 gcaatcacaa aatcttcagg cgttgggtgt tgtagaatca tccacataca ttgtacataa
      481 tctttggcat atccccagtc gcgcaacgaa tccatattac ccatatacaa tttatcttgc
      541 ataccttgag ctatacgtgc agcagcaagg gtaattttac gggtaacgaa tgtttcacct
      601 ctacgttcac tttcgtggtt aaataagatg ccatttacag caaacatacc atatgattcg
      661 cggtaatttt ttgtaatcca gaaaccatat tgcttagcaa ctccatacgg agagcgtgga
      721 taaaaaggtg ttgtttcttt ttgaggaatt tcttgtacta atccaaatag ttcagatgta
      781 gaagcttggt aaatcttaca ttctttttct agatttagaa tgcgtactgc ttctaacaaa
      841 cgtaatgtac ctacagcatc acactcggca gtgtattcgg gcacatcaaa actaactttc
      901 acatgacttt gagccgccaa gttgtatatt tcgtctggtt ttacatcttg aataatacgt
      961 actaaagaac ttgaatcagt catatcgcca taatgcaagt taatagctct ttttttcttc
     1021 atatccctaa cccactcagt aagatacaaa tgttcgatac gttctgtatt aaaagaagat
     1081 gaacggcgta aaacaccatg aacttcatag cctttttcta ataaaaattc agctaaataa
     1141 gatccatctt gacctgtaat tcctgtaatt aatgctactt tcatactatc tctttttcaa
     1201 tttgttatta atctgcaaat ttcaataatt ttatttgatt ctaattcata atacaatggt
     1261 aatcttacca aacaatcagc aaatctatct ccgttaggca atgtgcgacc atcatgtttg
     1321 tttgcataat aatcactact atgcaaactc aaatagtgaa agactgctaa aatatcattc
     1381 ttttttagtt tttttatcaa ttcagtacgt tgctctaaat tattacaaac taaataaaac
     1441 atgtgtgcat tatttgtagc ataatttggt ataataggca atcctattcc ttttatagca
     1501 ggcaaagata ctaaattatc ataatacaat tgccaaatct cttttcttct tctctgaata
     1561 tcatctaagt tttcgatttg tgcccacaaa aatgcggcta ttacttctga aggtaaaaaa
     1621 gaagaaccgg tgtctaccca accgtattta ttcacttcac cacgaaaaaa ctcagcccta
     1681 ttcgttcctt tttcccatat tatttctgca cggtgaataa atctctcatc attaatcgcc
     1741 aacattccgc cttcacccgc aataatattc tttgtttcgt gaaaagaaaa tgccgacaaa
     1801 tgaccaatgc tccctaaggg ctttcctttg tagtaactat caaccgcttg cgctgcatct
     1861 tctaccaccc aaatagaatg tttatttgct atttccataa tagcatccat atcgcaggca
     1921 acacctgcat aatgaacagg aacaatcact ttagttttag ctgttattag gtcttctaat
     1981 ttatttgcat ctatattagg attgtctgcc atgctatcag caaacacaat cgtagcacct
     2041 gcacgcacaa atgctaatgc agaagaaacg aaagtatagc ttggaacaat tacttcatcg
     2101 ccaggctgaa tattgcaaag tatggctgcc atttctaatg catctgtaca agatgttgta
     2161 agaagacatt ttttaaatcc ataacgttgt tcaaaataat cctgacaccg tttggtaaag
     2221 atgccattac ctgataattt tccactattt acagcttcgt aaatatagtg tgtttcttta
     2281 cctgtgaggt agggtttgtt gaatggtatc atatattatg caatttaata actattatct
     2341 aacaaaaaat cagcaatgtg acagtgatga atcccttgaa ttgactcgtt ccaaaaatca
     2401 tccattgtaa ctacatattt tgggtattga tcattgatag agagtaatac agagaattcg
     2461 cgttcaatag tctcaggatt ttcgagcttg taagccacct ggatatagat ttttttatcc
     2521 gctttttcag caataaaatc aatttctttg gagtctaatt tcccaacaaa cacattgtat
     2581 cctcttcgtt taagttctaa aaaaacaatg ttctccaaga ttcccgaaat taatgtatct
     2641 ttatacccca tggtagcata cagcaatgaa atatcgctca ggtaaaattt ttcctgcgtt
     2701 ttgagcagct cttttccttt tatatcgtat cgtgacaccc tgtgtataat aaacgctccc
     2761 tcaagggcat acagatagtt gtatacagta ttaatgtcga ttttacggaa ctgactttta
     2821 aaataatcgg ctacattttt acccgaaaaa gtattaccta tattactaaa tacatatttc
     2881 acaacccgct cgagcagttc cacatcgcgt attttatagc gttgaatggt atcgcgaagt
     2941 atgatagaag agtaaatatc aaagataatt ttatacatac tctcttcgga ataggacgaa
     3001 gtatgtacaa ttggaaaacc accctgcctc aaatagttta taaactgttt acgagtatct
     3061 tctgaaatat ttgttttata atgtgcgtta aaacttaaat actcactaaa cgatagcgta
     3121 taaatgggaa tttctacata cctcccagcc aaatgggtgg caagctccga cgaaagcaaa
     3181 tgtgaatttg agccggtaat gtatatatcg atatcgaagt cgacaagtaa agaattgatg
     3241 catttttccc aattagttac ttcctgcact tcatcaataa gcaaataata tttattttga
     3301 ggtattatct gtttacttat aaataaataa agtttttggg catccgtaaa ttctgagaat
     3361 gcaaaacttt cgaaattgat agcaatgatt tgttttgctg aaacaccatt cgagagtaag
     3421 tattttttaa gtaacgacaa caccgttgat tttccacaac gacgaatacc agtgattatt
     3481 ttaatcaatg gctggtcttt aaaacttagt attttatcta aatattggtc ccgtgctatc
     3541 atttttactg tttatggtta taaccacaaa gatagtgtat taattttaac tttttatgat
     3601 tataacccaa tattgtattt atcagcgaat tgttcatttt ccgaaagctt tgcacagctt
     3661 acatcttcat aaatatagtg tgtttctttt cctgttaggt agggtttatt gaatgtatca
     3721 tctcaattat ctaaatttgt ttaca
//
LOCUS       DAOSMY010000137         3723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24805_length_3723_cov_2.692748, whole genome shotgun sequence.
ACCESSION   DAOSMY010000137 DAOSMY010000000
VERSION     DAOSMY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24805_length_3723_cov_2.692748"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLD76_04065"
     CDS             <1..838
                     /locus_tag="PLD76_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27212.1"
                     /translation="DIIFGPAFSSQIPALANFAKLHKIKLVVPFSPTVPEIESNEYLY
                     QVITPQSDLHTSIAKEFSKVFSNKLVYFLQPRKDDLQDKKEFVNQLKVAFNQNNVSYR
                     TGFLGDFSASSLDSIANSTNKELVLIVPSTNLVVVTQLSNMIQSVQSSSIRIFSFSEW
                     HQLQIQDLYQLPHYTYTNYYIDFKDSNIHAFFTKLRNTYGIAGTQSVPNYALLGYDLA
                     NYFIGLLSNYDSNFESKLQEKNNGLLQMNLQFEKVSVNGGYQNKGIFLQSYDQQGITT
                     LK"
     gene            844..1497
                     /locus_tag="PLD76_04070"
     CDS             844..1497
                     /locus_tag="PLD76_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU27213.1"
                     /translation="MNTFFKSILIATVIFFNTNKIYSQFQKEISVGIIQGTVYPTVNF
                     DPIVTQEPILRYHGGLAIRYIAEPNVGIQAELTYSQRGWNEQSSSTGSFLKEVDYIEL
                     PLLTHIYVGKKSFRFFMQLGPKIRYAIQEKDKNTYLNSSSYQHINPIDNYFDYSLLGG
                     LGIEIRTHKLGFVQIETRYDFGIGDMYSNRKSDYFARSSNQAILASINYFFPSFYVK"
     gene            1511..2047
                     /locus_tag="PLD76_04075"
     CDS             1511..2047
                     /locus_tag="PLD76_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU27214.1"
                     /translation="MKSMYHLLQHRRSCRKYQPTSVSAQDIDTLLHAALMSPSSKRIN
                     PWEFIVVTDKELLEKLSHSKEHSSSFIAEAPLAIVIMANIEKSDVWVEDCSIAAILIQ
                     LVAEDLGLGSCWVQIRNRFTKNHKTSSNDYVKEVLHIPDTYAVEAIISIGYKVDERKP
                     FDEEKLQTEKIHYQHFTH"
     gene            2051..2836
                     /locus_tag="PLD76_04080"
     CDS             2051..2836
                     /locus_tag="PLD76_04080"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOU27215.1"
                     /translation="MIDVVLIGAGNVATHLAIGLHKRGIHIRQIFSRTTTSAQLLAEQ
                     VGATFTSSKSAIYTDASLYICSVKDSEISTVLTNIDFHNKLLIHTAGSVALDILAPFS
                     ANYGVLYPLQTFSKSKPINWEQCSLFIEANNEKNAEFILHIASLLSTKVQIIDSEKRK
                     NLHIAAVFACNFTNHLYTLTSELMLGSGANFNDLLPLIEETTNKLCTLSPYEAQTGPA
                     VRQDTNIVAEHIAQLAKTDINKATIYALLSKNIMQTHKMKNKE"
     gene            2833..3360
                     /locus_tag="PLD76_04085"
     CDS             2833..3360
                     /locus_tag="PLD76_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOU27216.1"
                     /translation="MNKNFKEYLPHIKAFAFDVDGVLSANCIPLYPNGEPMRMANIKD
                     GYVLQLAIKLGYPIAIITGGKTAAVRTRFEGLGIQDIYLGSSIKTIDFEHFIAKHGLH
                     PTDVLYMGDDMPDLPVMKLVGVPTCPSDAAAEIKAISCYVSNYRGGEGCVRDVIEQVL
                     KAQNKWLTDEHAFGW"
     gene            3384..>3723
                     /locus_tag="PLD76_04090"
     CDS             3384..>3723
                     /locus_tag="PLD76_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOU27217.1"
                     /translation="MKKFIQLIRLQNIISLLIFQALMYYGVVVAILTKYGLSFSYISL
                     EIILLFIASAFIAAAGYVVNDYFDTKIDTINKPDKVIIGKTLSRQQAAWIFQSLFGIG
                     ILAGIVLAFLL"
BASE COUNT         1307 a          626 c          617 g         1173 t
ORIGIN      
        1 agatataatt tttggtccag cttttagcag tcaaatacct gctttagcca attttgccaa
       61 attacataag ataaaattag tggtaccttt ttcaccaact gtaccagaaa ttgagtctaa
      121 cgaatacctt tatcaagtaa taactccgca aagtgattta cacacaagca ttgcaaaaga
      181 atttagcaaa gtatttagca ataagctagt ttattttttg cagcctcgta aagatgattt
      241 gcaggataaa aaagagtttg ttaatcaact gaaagttgct tttaatcaaa ataatgtaag
      301 ctatcgtact ggttttttgg gagatttctc agctagcagc ttagactcaa tagctaatag
      361 tacaaataaa gaacttgtac ttattgtgcc cagtaccaat ttagtcgtag taacacagtt
      421 gagtaacatg atacaatctg tacaatcctc ttctattcgt atatttagtt tttctgagtg
      481 gcatcaacta caaattcaag atttatatca attaccacat tatacgtata cgaattatta
      541 tattgatttt aaagatagta atatccatgc ttttttcact aaactgcgaa atacatacgg
      601 aattgctgga acacaaagcg tccctaacta cgcactgtta ggatatgatt tagcaaatta
      661 ttttatagga cttttatcta attatgatag taactttgaa tcaaaattac aagaaaaaaa
      721 caatgggtta ttgcaaatga atctgcaatt tgaaaaagta tctgtgaatg gtggatacca
      781 aaataaaggt atatttttgc aaagttatga ccaacaaggc attaccactt taaaatagca
      841 aaaatgaata ctttttttaa atcaatactg attgcgactg ttattttttt taatacaaat
      901 aaaatctatt cgcaatttca aaaagagata tccgttggta ttattcaagg aactgtatat
      961 cctactgtta attttgaccc tattgttaca caagaaccta ttctaaggta tcatggtggg
     1021 ctagctattc ggtatattgc tgaacctaat gttgggatac aagcggaact tacctatagc
     1081 caacgaggat ggaatgagca atcttcatct actggaagtt tcctaaaaga agtagattac
     1141 atcgaattac ctttgttaac acatatttat gtaggtaaaa agtcttttcg tttttttatg
     1201 caattaggac caaaaattcg ctatgctata caagaaaaag ataaaaatac gtatctcaat
     1261 tcatcttctt atcaacacat aaatcccatt gataattatt ttgattacag tttattaggt
     1321 ggtttaggaa tagaaatacg tactcataaa ttaggattcg tccaaataga aacacgatac
     1381 gattttggta ttggagatat gtatagtaat cgtaaatcag attactttgc tcgctctagc
     1441 aaccaagcaa tactcgcaag tattaattat ttttttccct cattttacgt aaaataaaac
     1501 aacaacaatc atgaaatcaa tgtaccactt acttcaacac agacgcagtt gcaggaaata
     1561 ccaacctaca tccgtttcgg cacaagatat tgacacctta ttgcacgctg ctttgatgtc
     1621 tccatcatcc aaacgtatta atccatggga atttattgtt gtaacagaca aagaattatt
     1681 agaaaaatta agtcatagca aagaacattc aagttctttt attgccgagg ctccgttagc
     1741 tatagttata atggcaaata tagaaaaaag cgatgtatgg gtggaagact gttcgattgc
     1801 tgctatctta atacaattgg ttgcagaaga tttaggattg ggctcttgct gggtacaaat
     1861 acgtaatcgt tttacaaaaa accataaaac aagctctaat gactatgtaa aagaagtatt
     1921 acacattcca gatacgtatg cagtagaagc tattattagt attggttaca aagtagacga
     1981 acgtaaacca tttgatgaag aaaaattgca aacagaaaaa attcattatc aacattttac
     2041 tcactaatat atgatagatg ttgtactaat aggtgcaggc aatgtagcaa ctcatttggc
     2101 aataggattg cataaaagag gtatacatat ccgccagatt tttagtcgca ctacgacctc
     2161 tgctcagtta ctagcagaac aggttggagc tacatttact tcttctaaat cagctattta
     2221 tacagatgct tcgttgtata tttgcagtgt aaaagacagc gaaattagta cggtcttaac
     2281 aaatatcgat tttcataaca aattacttat tcatactgct ggtagtgtag cattagatat
     2341 attagcccct ttttctgcta attatggtgt actgtatccg ttacaaacgt tttcaaaatc
     2401 aaagccaatt aattgggaac aatgtagcct attcattgaa gctaataacg aaaaaaatgc
     2461 agaattcatt ttacacattg cttcactatt gagcacaaaa gtacaaatta ttgattcaga
     2521 aaaacgcaaa aacttacata ttgcggctgt attcgcttgt aattttacaa atcatttata
     2581 tacgctaact agtgaattaa tgttgggaag tggagcaaac tttaacgatt tactaccttt
     2641 aattgaggaa acaacaaaca aattatgtac actatctcct tacgaggcac aaacgggtcc
     2701 agcagtacga caagacacaa atatcgtagc agaacatatt gcacaactag caaaaaccga
     2761 catcaataaa gcaacgattt acgccttatt aagtaaaaat ataatgcaaa cccataaaat
     2821 gaaaaacaaa gaatgaataa aaattttaag gaatacttac cccatattaa agcatttgct
     2881 tttgatgtag atggtgtttt gtcagccaat tgcatccccc tgtatcccaa tggggaacct
     2941 atgcgtatgg caaatataaa agatggctat gtattacaat tggctataaa attaggatat
     3001 cctattgcca tcattactgg aggaaaaact gctgctgtac gtactcgatt cgagggatta
     3061 ggcatacaag atatatattt agggtcatcc ataaagacga tagattttga acattttatt
     3121 gccaaacacg gattacatcc tacagacgtg ctttacatgg gagatgatat gcccgatttg
     3181 cctgtaatga aactcgtagg tgtgcccacc tgccctagcg acgcggctgc agaaattaaa
     3241 gcaatatcct gctacgtatc aaactaccgt ggaggcgaag gctgtgtacg cgatgtgata
     3301 gaacaagtat taaaagcaca aaacaaatgg ttgaccgatg aacatgcatt cggttggtaa
     3361 aaaacaaata ataataacac actatgaaaa aatttattca attaattcgc ttacaaaaca
     3421 ttattagttt acttatattt caggctctta tgtattatgg cgttgttgtt gccattttaa
     3481 ctaaatatgg tctatccttt tcgtatataa gccttgaaat aattttactg tttattgcgt
     3541 cagcttttat tgcagctgcg ggatatgttg tcaatgacta ttttgacaca aaaattgaca
     3601 cgattaataa accagataaa gtaatcattg gcaaaacact atcgcgtcag caagcagcat
     3661 ggatttttca atcccttttt ggtattggaa ttttggcagg tatcgtattg gcatttctgt
     3721 tga
//
LOCUS       DAOSMY010000138         3719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24836_length_3719_cov_3.489629, whole genome shotgun sequence.
ACCESSION   DAOSMY010000138 DAOSMY010000000
VERSION     DAOSMY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24836_length_3719_cov_3.489629"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..2855
                     /gene="glgP"
                     /locus_tag="PLD76_04095"
     CDS             288..2855
                     /gene="glgP"
                     /locus_tag="PLD76_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU27218.1"
                     /translation="MKLKVSNVNYPNWRAITVNFKVPKEFEKLQEISKNLWWVWNYEA
                     TDLFKEIDKDLWIEAGGNPVLFLQKLSAEKYKELAKDKAILKKIDKIYADFKAYINVK
                     PSTTIPSVAYFSMEYGLTDILKIYSGGLGVLAGDYLKEASDCNVDMVAVGFMYRYGYF
                     TQALSINGEQVALYEAQQYESLPMEQLLDKNGDPVVISVPYPGRTVYANLYKVAVGRV
                     DLILLDTDTELNNDLDRTITHQLYGGDNEHRLKQEIMLGIGGIAALKAIGLEKDVYHC
                     NEGHAAFLNVQRLIDLMQDKQLSFNEAKEIVRVSCLFTTHTPVPAGHDTFEEPLFERY
                     MFDFATRLGLSWDDFMNMGRETPGDLTEKFSVTVFACNTAQEVNGVSWLHGKVSQDMF
                     KHIWEGYFAEESHVSWVTNGVHYPTWAASEWQKVHEKALGKEYMSDQSNKAIWSKIYD
                     VDDKEIWDTRQVLKLKLVEYIKAKFASNWLKNQGDPSRIVSVLERINPNALTIGFGRR
                     FATYKRAHLLFTDLERLSRIVNNPDFPVQFIFTGKAHPADGGGQKLIQRVVEISRRPE
                     FLGKVIFLENYDMAIAKRLISGVDIWLNTPTRPLEASGTSGEKAEMNGVLNFSVLDGW
                     WYEGYKKGAGWALTDKRTYQNQEHQDQLDAATIYSMLENEIVPLYFAKNSKGYSPEWV
                     QYIKNSFALIAPEFTMKRMLDDYIERFYIKLAKRGKSLKAKDFAKAKEIASWKENVAS
                     KWDAIEVVSVELSENLHAQAEVGQSQKATIVVDVKDASLVNSIGVEEVVTHPNKEGVD
                     TFFSAKEVNLVKTEGTLLTFEMNQKVENGGSFKYAFRMFPKNSDLPHRQDFCYVRWF"
     gene            complement(3059..>3719)
                     /locus_tag="PLD76_04100"
     CDS             complement(3059..>3719)
                     /locus_tag="PLD76_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU27219.1"
                     /translation="VDCALLYNPRDFYVTNVKSYPYYTSSDSSRRSRDQLVVSGILSG
                     EEIHVIVNHWPSRRGGEVNSRPRRVAAAQLTKHIADSIFTIDKNAKIIIMGDLNDDPI
                     NESVKDVLNAKQKKSETPEGGLYNTTWELYNKGIGSLAYGDAWNLFDQIIVSSGLIGK
                     DKAELTFWKAEIFQAPFLIRQEGQYKGYPFRTHAGGVYQNGYSDHFPSIIYLIKRIDN
                     K"
BASE COUNT         1216 a          548 c          720 g         1235 t
ORIGIN      
        1 agagcattag ccttccaagc tgagggtcgc gggtttgagt cccgttttcc gctctatgaa
       61 gggatgataa tgaaacgtat catcccttct ttttgttttt atatttcaaa cgtttgcggt
      121 acttgttgta gcctaaattt tgtctaagtt attaacaatg aggataattt tatgaataaa
      181 aaacttgttt gttcttgcaa aaatagtatc tttgccctcc gttggagtaa aatgccaaat
      241 atagtaatct aaatagtaga aaaaattatt tcttaaccta aatgaatatg aaactaaaag
      301 ttagtaatgt aaattatcct aattggagag caattaccgt aaattttaaa gtgccaaaag
      361 aatttgagaa attacaagaa atttcaaaaa atttatggtg ggtatggaat tatgaagcaa
      421 ctgatctttt taaagaaatt gataaagatt tgtggattga ggcaggagga aatcctgtgt
      481 tgtttttgca gaaacttagt gctgaaaaat acaaagaatt agcaaaagat aaagctattt
      541 tgaaaaaaat agataaaatt tatgctgatt ttaaagctta tataaatgtt aaaccaagta
      601 caactattcc ttctgttgct tattttagta tggaatatgg tttaactgat attttaaaaa
      661 tttactcagg tggtttgggt gtgttagcag gtgattactt gaaagaggct agtgattgca
      721 atgtagatat ggtagctgtt ggttttatgt atcgttatgg atactttaca caagctttat
      781 ctattaatgg cgagcaagtt gcattgtacg aagctcaaca atatgagtca ttaccaatgg
      841 aacaactcct tgataagaat ggagatccag tagttatttc agttccatat ccaggtagaa
      901 ctgtgtatgc taatctatat aaagtagctg ttggacgtgt tgatttgatt cttttggata
      961 cagataccga attgaataat gatttggatc gtactattac acatcaatta tatggcggag
     1021 acaacgaaca tcgcttgaaa caagaaatta tgcttggtat tggtggtatt gctgctctta
     1081 aagctattgg cttagaaaaa gatgtttatc attgtaacga aggtcatgct gcatttttaa
     1141 atgtacaacg cctaattgat ttgatgcaag ataaacaact ttcttttaat gaagctaaag
     1201 aaattgtacg agtgtcatgt ttatttacaa ctcatactcc agttcctgct ggacacgata
     1261 cattcgaaga accattattt gagcgttata tgtttgattt tgctactcgt ttaggattat
     1321 cttgggatga ttttatgaat atgggtcgcg aaacgcctgg tgatttgaca gaaaaattct
     1381 ctgtaacagt atttgcttgt aatactgctc aagaagtgaa tggtgtaagc tggctgcacg
     1441 gaaaagtttc tcaagatatg ttcaaacata tttgggaagg ttattttgca gaagaatcgc
     1501 atgttagttg ggttactaat ggtgttcatt atccaacatg ggcagcatct gaatggcaaa
     1561 aagtgcacga aaaagcttta ggtaaagagt acatgtctga ccaatcaaac aaagctattt
     1621 ggagcaaaat atatgatgta gacgacaaag aaatttggga tactcgtcaa gttctcaaat
     1681 taaaattggt tgaatatatt aaagcaaaat ttgcttctaa ctggttgaaa aatcaaggag
     1741 atccttctcg tattgtaagt gtgttggaaa gaatcaatcc taatgcttta accattggtt
     1801 ttggtcgtcg ttttgctact tataagagag cacatttatt gtttactgac cttgaaagac
     1861 tttctcgcat tgtgaataat cctgattttc ctgttcaatt tatttttaca ggtaaagctc
     1921 acccagctga tggtggtggt caaaaattaa ttcaacgtgt tgtagaaatt tctcgtcgtc
     1981 cagaattctt aggaaaagta attttcttag aaaactatga catggcaatt gcaaaacgtt
     2041 taatttcagg tgtggatatt tggttaaata caccaactcg tccgcttgaa gcttctggta
     2101 cttctggtga aaaagcagaa atgaacggtg tattaaattt ctctgtactt gatggatggt
     2161 ggtatgaagg ttacaaaaaa ggtgcaggtt gggctttgac agacaaacgc acataccaaa
     2221 atcaagaaca ccaagatcaa ttagatgcag caactattta cagtatgctt gaaaatgaaa
     2281 tagtaccatt gtactttgca aaaaatagca agggttattc tcctgaatgg gtgcaatata
     2341 tcaaaaattc atttgcgtta attgctccag aatttacgat gaaacgtatg ttggacgatt
     2401 atattgaaag attctatatt aaattagcta aaagaggtaa atctttaaaa gcgaaggatt
     2461 ttgctaaagc aaaagaaatt gcatcttgga aagaaaatgt tgcttctaaa tgggatgcta
     2521 ttgaagttgt ttcggtagaa ctatcagaaa accttcatgc acaagctgaa gttggtcaat
     2581 ctcaaaaagc tactatcgtt gttgatgtaa aagacgcatc tttagtaaat tcaattgggg
     2641 tagaagaagt ggtaacacat cctaataaag aaggtgtgga tactttcttc tcagctaaag
     2701 aagtaaatct agtgaaaaca gaaggtacct tacttacttt tgagatgaat caaaaagtgg
     2761 aaaatggggg atcgtttaaa tatgcattcc gcatgttccc taaaaattca gatttacctc
     2821 atcgtcagga tttctgttat gtacgttggt tttaattaaa caaataatac tattttctca
     2881 agaagctgtc tcaaaaggac agcttctttt gttatgccaa gttatgattt tctgtagagg
     2941 atagataagt gagattagtt aattatatag gtattatata accaaaataa tacataaaat
     3001 aaaaatagag gatggaacgt attcgtcatt ccatttgctt cgtatggtaa ggtttatttt
     3061 atttgttatc aatacgttta atcaaataga taatagaggg gaaatgatca ctatacccgt
     3121 tttgatagac tccaccagca tgagttctaa aaggataacc cttatattga ccttcttgac
     3181 ggattaaaaa tggtgcttga aaaatttcag ctttccaaaa tgtaagctct gctttgtctt
     3241 tgccgattaa acctgaggaa acaataattt gatcaaatag attccaggca tctccataag
     3301 ctaatgagcc tatgccttta ttgtaaagct cccaagtggt attatacaat ccgccttcgg
     3361 gtgtttcgct ctttttttgt tttgcattca gtacatcttt aacactttcg ttgattgggt
     3421 cgtcatttaa atcacccata atgataattt tagcattttt gtcgatagta aaaattgaat
     3481 cagcaatatg cttggttagt tgagccgctg ctactcttcg tggacgtgaa ttgacttctc
     3541 cacccctacg cgaaggccag tgatttacga ttacatgaat ttcttcgcca cttaatatgc
     3601 ctgaaactac taattggtcg cgacttctac gagatgaatc actgcttgtg taataaggat
     3661 aactttttac attggttacg taaaaatctc gtggattata tagtaatgca caatctact
//
LOCUS       DAOSMY010000139         3711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24890_length_3711_cov_3.456783, whole genome shotgun sequence.
ACCESSION   DAOSMY010000139 DAOSMY010000000
VERSION     DAOSMY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24890_length_3711_cov_3.456783"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLD76_04105"
     CDS             complement(<1..1718)
                     /locus_tag="PLD76_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27220.1"
                     /translation="MKNRILHQLLFLVFSLFFLLTSCIDDSYKIDDIDTTVGLDMALV
                     GPIGKSKITLSSVFPDDLENYELLIRNDSIFIVLHDTVDFGSTFIDDIKIPKWKYTKH
                     IPVIDSDLLPTTSVDVDVTLDYEFDINTNPNERIDSLVFNTSVLNFQFTSNYDYSPAA
                     QMEVIFDSDELVLNPSLYPENKIVLSLVQGTINQNIDLTGAKLFFPNSSKKIKIRYKV
                     HVETNTPFVPGTEPIDVAIDFDATTPRIVYGYIGQNRDIWGGEIVRPFDFTSLFQGVD
                     FFLPFYNPQIYITAESNIGIPAQYTLDWVKATDTNTGEVVYADFEGSPNTSFVLNYPV
                     ISEIENKSIQELLSFDSRTLDKRHTKLFDREYGATNRLFQINANELAYKFSVRTVNDP
                     AASVHYFLNDSKMSLYIKAMIQLNFEGNDNPAKSFHVNLSDTLDIDFAQIIDVDQVDT
                     ENEIVARIKLNYKNALPIGAFVSLSFLDENKEKVQYNNETLEKEFTIEPAQVKPDGTV
                     LAPLPNTSITINLTADEYTNYISKTKKLAFTYSMKGNDGKNVQIKASDWLELKASFYV
                     SAKTKVT"
     gene            complement(1800..3197)
                     /locus_tag="PLD76_04110"
     CDS             complement(1800..3197)
                     /locus_tag="PLD76_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU27221.1"
                     /translation="MLTRYIENKTTGYLGFTPTPEQAELISQLALFLAEPQKRHTFLL
                     KGYAGTGKTSVVSAIVKLLDELQQPVILLAPTGRAAKVLSSYSGKQAATIHKHIYRKK
                     TAIADAPFDIAYNSYKNALFIVDEASMVANTGDGLFGSGRLLDDLITYVFSAENCHLL
                     LLGDTAQLPPVMQEESEALDTKILEKYDLYVQEYTLTQVVRQAEASGILRNATHIRQM
                     ISNQINIHSFILHTDSDVIRVSGQDFVETLYTAYRDGVDETILITRSNKKAAMYVEGI
                     RARILQREELLSAGDLVMVVKNNYFVGKEYQGFEFIANGDIAEVLRVRSYSQLYGFTF
                     ADVVVRFLDYDWEVDAKILVDVLSCVTPAELQEMNKKLFKTVEEDYAHIGNKRTRYKE
                     MKNDIYLNALQLKFAYAITCHKAQGGQWKHVFVDMGMIKKTEIDTSFLRWLYTASTRA
                     TQKLYLINFDDAFFC"
     gene            3377..>3711
                     /locus_tag="PLD76_04115"
     CDS             3377..>3711
                     /locus_tag="PLD76_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27222.1"
                     /translation="MDKIFKLILVAVSIVLGYLCVQSILIPIKFNEEKSFREKVVIAR
                     LADIRRAQVEYRTAKGVYTNSFDTLIAFVRQGKAPIILKEGSLTDKQLEAGLTEKEAV
                     RQGIIKRDTT"
BASE COUNT         1242 a          681 c          549 g         1239 t
ORIGIN      
        1 gttactttcg tttttgcaga cacataaaaa ctcgctttca attctaacca atcacttgct
       61 ttgatttgta cgttctttcc atcatttcct ttcatagaat atgtaaaggc aagttttttt
      121 gtttttgaaa tatagtttgt atattcatct gctgtcaaat ttattgttat acttgtattt
      181 ggcaaaggag ctaggacagt accatcgggc ttaacctgag caggttcaat ggtaaattct
      241 ttttctagtg tttcattgtt atattgaact ttctccttat tttcgtccaa aaatgaaagc
      301 gatacaaatg ctccaatagg cagtgcgttt ttataattca atttaatcct tgcaacaatt
      361 tcattttctg tgtctacttg gtcaacatct atgatttgag caaaatcgat atctaaggta
      421 tcggacaaat taacatgaaa acttttggct ggattatcat ttccttcaaa atttagttgg
      481 atcatggctt taatatataa agacatttta ctatcattca aaaaataatg aacagatgcc
      541 gctggatcat ttacagttct taccgaaaat ttgtaagcca attcgttcgc atttatttga
      601 aataaacgat tagttgcacc atattctcta tcaaagagtt tcgtatgcct tttgtctaat
      661 gttctactat caaagcttag caattcttgt attgatttat tttcaatttc actaatgact
      721 ggataattaa gcacaaaact cgtattggga gagccttcaa aatcagcata taccacctca
      781 cccgtatttg tatcagttgc cttaacccaa tctaaagtat actgtgcagg tataccaata
      841 ttactttctg ctgttatata aatttgcgga ttgtaaaatg gcaaaaagaa atcaaccccc
      901 tgaaacaatg aagtaaaatc aaacggtcta actatttctc ctccccaaat atctctattt
      961 tgaccaatat atccataaac aatacgaggt gtcgtagcat caaaatcaat ggctacatca
     1021 atgggttctg tacctggcac aaaaggtgta tttgtttcaa catgtacctt gtaacgaatt
     1081 ttaatttttt tcgacgaatt tgggaaaaac agctttgctc cagttaagtc aatattttga
     1141 tttattgtac cttgtaccag agataacaca attttatttt caggatagag agaaggatta
     1201 agaacaagtt catcagaatc aaaaataacc tccatctgag cagcgggaga ataatcgtag
     1261 ttagatgtaa actgaaaatt taataccgac gtattgaaaa caagactatc tatccgctca
     1321 ttcggattgg tattgatatc aaattcatag tccaacgtta catcaacatc gacactcgtg
     1381 gtaggcaaca aatcgctatc aatcacaggt atatgctttg tatacttcca tttaggtatt
     1441 ttaatatcat caataaaagt acttccaaaa tcaacagtat cgtgtaatac aataaaaata
     1501 ctgtcgttac gaatcaataa ttcgtaattt tcaagatcat caggaaaaac agacgaaagc
     1561 gtaattttag atttacctat aggaccaact aacgccatat ccagtcctac cgtagtatct
     1621 atatcatcaa ttttataact atcatctata cagcttgtta ataaaaaaaa taaagaaaaa
     1681 actaggaata aaagttggtg tagtattcta tttttcatac gcataaattt aatggctcat
     1741 ttttctttct aaattacttg tacgaagata gtacaaaatt tgataaatcg aaacaatttt
     1801 taacaaaaaa acgcatcatc aaaatttatt aaatacaatt tttgggtagc tcgagtacta
     1861 gcagtataca accaacgtaa aaatgaggtg tcaatttctg tttttttaat cattcccata
     1921 tccacaaaaa catgtttcca ttgtcctcct tgtgctttat gacaagtaat tgcataagca
     1981 aactttagct gaagtgcatt caaataaata tcatttttca tttctttata cctagttctt
     2041 ttattgccaa tatgtgcata atcctcctca actgttttaa ataatttttt attcatttcc
     2101 tgtaattcgg caggagtaac gcaagacaag acatctacca aaattttggc atccacctcc
     2161 caatcgtagt ctaaaaaccg aacaactaca tcagcaaaag taaatccgta taattgtgaa
     2221 taggaacgta cacgcaaaac ctctgctata tctccgtttg caataaattc aaacccctga
     2281 tactccttgc caacaaaata attatttttt accaccatta ccaaatctcc tgcagataac
     2341 aactcttctc tttgtaatat cctagcacga attccctcca catacatggc cgcttttttg
     2401 ttagaacgtg taataagaat ggtttcgtct actccatcac gatatgctgt atacaaagtt
     2461 tctacaaaat cttgacctga cacacgtatc acatcactat ctgtgtgtaa aataaaactg
     2521 tgtatattta tctgattact tatcatttgt cggatatgtg tggcgttgcg taaaattcca
     2581 cttgcttctg cctgacgtac cacttgtgta agggtatatt cttgcacata taaatcatat
     2641 ttttcaagga ttttagtgtc caatgcctca ctttcttctt gcattacggg tggtaactgg
     2701 gcagtatcac ccaacaataa taaatggcaa ttctcagcac taaacacgta ggtaatcaaa
     2761 tcatccagta aacgaccact tccaaacaga ccatctcctg tatttgccac catagatgct
     2821 tcatctacaa taaataatgc atttttataa ctattatatg ctatatcaaa aggagcatca
     2881 gctatggctg tttttttgcg ataaatatgt ttatgaatgg ttgctgcttg tttgccagaa
     2941 tacgaagaca agactttggc ggctcttcct gttggtgcca ataaaataac gggctgttgc
     3001 aattcatcta acaatttaac aatagcactt accacagatg ttttacccgt tcctgcatat
     3061 cctttaagta aaaaggtatg gcgtttttgc ggttcagcca aaaaaagagc aagttgggat
     3121 atcaactctg cttgttctgg cgtaggagta aatcctaaat agcctgtggt tttattttct
     3181 atatatcgtg taagcattat cagacttata ttaacgagta aaaataagat atacgtgaaa
     3241 tatttagttt gacacaaaag taatgaatta aaaaaaaatt agctctacac atctattttt
     3301 ttttcaaata aagataatta gttccaaatt ctttcttaac tttgcgtcat tctaaaaaca
     3361 aaaccttgta acaccaatgg acaaaatttt taaactaatt ttagttgctg taagtattgt
     3421 attaggctat ttatgcgtac aaagtatttt aattcctatc aaatttaacg aagaaaaatc
     3481 ttttcgtgaa aaggtagtta tagcacgttt agcagatatc agaagagctc aagttgaata
     3541 tcgcacagcg aaaggcgtat atacaaatag tttcgataca cttattgctt ttgttagaca
     3601 agggaaagct cctattattc ttaaagaagg aagtttaacc gacaaacaat tggaagcagg
     3661 tctgactgaa aaagaagctg ttcgtcaagg aatcatcaaa agagatacaa c
//
LOCUS       DAOSMY010000140         3710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_24907_length_3710_cov_3.107798, whole genome shotgun sequence.
ACCESSION   DAOSMY010000140 DAOSMY010000000
VERSION     DAOSMY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24907_length_3710_cov_3.107798"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3710)
                     /locus_tag="PLD76_04120"
     CDS             complement(<1..>3710)
                     /locus_tag="PLD76_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOU27223.1"
                     /translation="IEGWVVDRITGKPIKNATVTVHQVRYQRNNVDVKKVASTNTNKN
                     GLATFSLQHNNAYYLSVSQGEDTSTPIGNVYVYGKQKENTPPDMQQHIALFTDRALYR
                     PAQSVYVKGIAWNVKEDSVILEKNKKIHIRLRDANYQIIAEKQVQTSSYGSFNTDFIL
                     PKSGLTGSFTLETENGSSSFLVEEYKKPTFKAEIKRIDKEVLFNNPVRINGNAETYMG
                     FPLQTAHGKYRIVRQQFSWRRYFPIHTTENVAEGVFKTNDKGEFTFDFIPIKPDTDES
                     AYYHYLVYVDITDKAGETQQVSSSFSIGESAIYIQTNLPAIIQKEKITPIAVNVTNVN
                     GEMLHQSGKIAIYALKESSNNDTPVDSLQLSKCVYEGSFVSGIPLSLNIVSNLPSGRY
                     KLISSTKTQSLTEVENIHFFTLFSVKDKKPPFKTSNWMYALSDTCSVGEMASTIIGSS
                     ANVLVMYELVGEDGLICRQFITLNNESKHIQFPYTKNCGNSVEMRFTSVVNNTLYQQS
                     NRIVKRQPSKKLSIQYITFRDKLQPGNEEEWQIHVKDANGVNQQAEVLVCMYDASLDA
                     IVPHQWQFNPFVSHQWYVPSWNATPSFSRSNYATGIVPFKDEKGFELPKINLFDFQYP
                     GYCRYAMPYMAVGGNMLRKSLEVIDDVMDEVVEDKVLSTETTASASSQLSQKMPIRKE
                     FAETAFFYPAIKTDNEGLATVRFTVPESLTTWKIMTLVHNADAYYGQQTSYVYTQKAI
                     SVKPNLPRFVQQSDSIVLTATIYNMLDSLAKTTAKWEIRDAQTDSILLSNEEKVTLNP
                     HQSVVIKLPLFVPTNSSLWVVKVMVANDSYSDGEQHYLPILSSKKLVTESMPFTMYKE
                     GEYTFEFDKLKNNTSTTLSHKRYTIELSPNVAWYALQALPVVAEPEYDNAISLMSSYY
                     ANILGEFVVANTPLFNQYIQLYANKEESPLVKNKELKNLLNSESPWIQDALQETEGLS
                     KLQLYLDANQLHHKQQELWKKLQELQAPTGGFSWFAGMPENEMVTLFIAEKIAKLVSI
                     QALDSTIFGTEMMQQAVAYIDYQIIKSYNTYIKHSTSKPFVVDAYTLQALYVRSLFST
                     IPKSEELNKSIIYFNAVLKQNWTAYSLYGKSQVARILFRNNEKKLAQSIVASLLEFAT
                     ENKDKGIFWANNTAGYSWYQMPIVTHVAIMEAIAEIKPNKEIEDKLKIWLLTNKQTNA
                     WNNPIATVDAIYALVFTGNSMSIVQPSSIIVKV"
BASE COUNT         1085 a          675 c          622 g         1328 t
ORIGIN      
        1 acttttacaa ttatactaga cggttgaaca atactcattg agtttccagt aaacactaaa
       61 gcataaatag catctactgt agctattgga ttattccatg cattggtttg tttattggta
      121 agtaaccata ttttaagttt gtcttctatt tctttgttag gtttaatttc ggcaattgct
      181 tccattatag caacatgcgt tacaatgggc atttgatacc aagaatagcc ggcagtatta
      241 tttgcccaga atattccttt atccttgttt tcagtcgcaa attcgagtaa tgaagcaaca
      301 atactttgtg ctaatttctt ttcattattt ctaaataaga ttctggcaac ttgcgatttt
      361 ccatacaaag aataggctgt ccaattttgc tttaaaactg cattgaagta tattattgat
      421 ttattcagct cttctgactt tggaatagtt gaaaaaagcg acctcacata caatgcttgt
      481 agtgtatatg catcaacaac gaatggtttg cttgtagaat gttttatgta agtattgtat
      541 gacttgatta tctgataatc aatatacgct actgcttgct gcatcatctc tgtaccaaaa
      601 attgtggagt ctaaagcttg tatcgacact aattttgcta ttttctctgc tataaacaac
      661 gttaccattt cattctcagg cataccagca aaccaagaaa aaccccctgt tggtgcctgt
      721 aattcttgca attttttcca caattcttgt tgtttatgat gcaattgatt tgcgtccaaa
      781 tatagttgca acttagataa cccttccgtt tcttgcaggg catcttgtat ccacggactt
      841 tctgagttta acaaattttt cagttcttta tttttaacta agggcgactc ttccttattg
      901 gcataaagct gaatatattg attaaataat ggtgtatttg ctacaacaaa ctcaccaaga
      961 atattagcat aataacttga cataagagaa atagcattat cgtattctgg ctcagctact
     1021 acaggtaatg cctgtaaggc ataccatgct acattgggag ataattcaat cgtataacgt
     1081 ttgtgtgata atgttgtaga tgtattgttt ttcagtttat caaattcaaa tgtatattcc
     1141 ccttccttat acatagtaaa aggcatactc tctgtaacta attttttgct cgacaagatg
     1201 ggtaaataat gttgttcccc atcgctatac gagtcatttg ctaccatcac ttttactacc
     1261 caaagagagg aattagttgg cacgaatagt ggtaatttaa ttacaaccga ttgatgtgga
     1321 ttcagagtta ctttctcttc attggatagt aatatactgt ccgtttgtgc atctcttatt
     1381 tcccatttag cagtagtttt agctagtgaa tccaacatat tgtagatagt agcggttaac
     1441 acaatgctat cactttgttg aacaaaacga ggtaaatttg gtttcacaga aatagctttt
     1501 tgggtataca catacgatgt ttgttgtcca taatacgcat ctgcattatg aacgagtgtc
     1561 attattttcc atgttgttaa agactctgga actgtaaaac gcaccgttgc caatccttca
     1621 ttatctgttt ttatggctgg ataaaagaac gctgtttctg caaattcttt acgaataggc
     1681 attttttgag ataattgcga agatgcagaa gcagttgttt cagtagaaag aactttgtct
     1741 tctactactt catccattac atcatctatt acctctaatg atttccgcaa catattgcct
     1801 ccaacagcca tatacggcat agcataacga caatacccag gatattgaaa gtcaaacaga
     1861 ttaattttgg gtaattcaaa acctttttca tccttgaaag gaacaatacc tgtagcataa
     1921 tttgaacgag aaaaagaagg tgttgcattc caagatggaa cataccactg atgtgaaaca
     1981 aacggattaa attgccactg atgcggtaca atagcatcta acgaagcatc gtacatacac
     2041 acaagcacct ctgcttgctg attaactcca tttgcatctt ttacatgtat ttgccactct
     2101 tcttcgtttc ctggttgtaa tttatctcga aaagtaatat actgaataga tagttttttt
     2161 gaaggttgcc gttttactat tcggttgctt tgctgataca aagtattatt tacaacagat
     2221 gtaaaacgca tttctacact attgccacaa tttttagtat acggaaattg aatatgttta
     2281 ctttcgttgt ttaaagtaat aaactgacgg cagatgagtc cgtcttctcc aactaattca
     2341 tacataacca acacattagc tgaagaacca atgatggttg aagccatttc tcccacagag
     2401 caggtatctg ataaagcata catccagttt gatgttttga atggaggttt tttatctttt
     2461 accgaaaata atgtaaagaa atgtatgttt tctacttctg ttaggctctg cgttttcgtt
     2521 gagcttatta gtttgtatct acctgaaggt aaattggata ctatatttaa cgaaagtggt
     2581 attcccgaca caaaagagcc ttcatataca cacttactca gttgtagact atcaacagga
     2641 gtatcattat ttgagctttc ttttaaagca taaattgcaa ttttgccact ttgatgaagc
     2701 atttctccat ttacattggt aacatttacc gctattggag ttattttttc tttttgaata
     2761 attgcaggaa gattcgtttg tatatatatg gcactttctc cgattgaaaa actcgaagac
     2821 acttgttgtg tttcaccagc tttgtcggta atatcaacat atacaagata gtgataataa
     2881 gcactctcgt cggtatcagg ttttatcgga ataaaatcga atgtaaactc gcccttatca
     2941 tttgttttga aaacaccttc tgccacgttc tctgttgtgt ggataggaaa atacctacgc
     3001 caagaaaatt gttgtcgaac aatgcggtat ttaccatgtg cagtctgtaa aggaaagccc
     3061 atatatgttt cagcatttcc atttatgcga acaggattat taaacaatac ttctttatct
     3121 attcgcttaa tttcagcttt aaaagtaggt tttttatatt cttctactaa aaatgagcta
     3181 gatccatttt ctgtttcgag tgtaaagctc cctgttagcc ctgattttgg taatataaaa
     3241 tcagtattaa aagagccgta acttgatgtt tgcacttgtt tttctgcaat aatttggtag
     3301 tttgcatcac gcagtctaat gtgtattttt ttgttttttt ctaaaattac agagtcttct
     3361 tttacattcc acgcaatccc ttttacatac acactttgtg ctggacgata caatgcccta
     3421 tctgtaaaca gtgcaatatg ttgttgcata tctggcgggg tattctcttt ttgtttgccg
     3481 tacacataca cattaccgat aggcgtgcta gtatcctctc cttgcgatac gcttaggtaa
     3541 taagcattgt tatgctgtaa cgaaaaggta gctaatccgt ttttattcgt attggttgat
     3601 gctacttttt ttacgtcaac attgttacgt tgatagcgaa cttgatgaac agtaacagtt
     3661 gcgtttttga taggtttacc agttattcta tctactaccc acccctctat
//
LOCUS       DAOSMY010000141         3693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25063_length_3693_cov_3.860088, whole genome shotgun sequence.
ACCESSION   DAOSMY010000141 DAOSMY010000000
VERSION     DAOSMY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25063_length_3693_cov_3.860088"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1279)
                     /locus_tag="PLD76_04125"
     CDS             complement(62..1279)
                     /locus_tag="PLD76_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU27224.1"
                     /translation="MNHLINRQNYIQQLEKLKDKHIIKVITGLRRSGKSTLFEIFIQQ
                     IRDSGVSARNVQQYNFEKPIYPQEYTWRDIYKDILAKTDKDNMNYIFLDEPQQISEFE
                     RLIDALYVERYIDLYVTGSNAYFLSGDLATLLSGRYITIHILPFSFAEFCESQKDTTM
                     NKYELFNHYLYETSLPQGVFLRNQGTDIQNMYIQDVYNTIVEKDIRQRYTIQNSRSFD
                     NLSKFLMGAIGSVVSPNNISKAMKQDRQAIHHNTVEKYIEYLVSAYVFYQVNRFDIKG
                     KQQLATQEKYYLVDIGFRNLKLGKFQYQDVGHILENIVYLELNRRGYQIWIGKIGEYE
                     VDFIVKNALNKFEYYQVAWSITDPKTAEREIRPLKSISDNYPKYILSTDIITAEIEGI
                     EHINIVDWLLKKE"
     gene            1775..2386
                     /locus_tag="PLD76_04130"
     CDS             1775..2386
                     /locus_tag="PLD76_04130"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOU27225.1"
                     /translation="MAQSIENKVLEHLKYRQRGKIYFTNDFATLGTSESIRKSLSNLV
                     KKDILIRLTQGIYLYPKIDKDLGVLYPSVDDVCRAIAKRDKARIEPTGIFALHSLGLS
                     TQIPVNVVYLTDGIPRKIKYGNRTIKFKKTAPKNLAMKGKISSLVIAALKQIGKENVS
                     QEVELQLTKALKKESVENVKHDAQLAPVWISKLIFQLVNNIEK"
     gene            2383..3387
                     /locus_tag="PLD76_04135"
     CDS             2383..3387
                     /locus_tag="PLD76_04135"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOU27226.1"
                     /translation="MNWTELSTTRRVEVLKHLENKTGLNATSIEKDWWVTLALNALFN
                     GLYGSHLVFKGGTSLSKCWRIIDRFSEDIDLAIDREFLGFSGKLTKREIGKKLRKASC
                     AFIRNELKADIERFILNCGINNDQFKLEVNITTESNVDPETLYLHYESVLDNSNYVFN
                     TVKIEVGSRSLMEPSEEVAIQSIIGEHFPKVDFSDKEFKVKAVLPQRTMLEKAFLLHE
                     MLQSPSSGREINRMSRHLYDLEKLMDSAFCDDALQNRELYYDILNHRKLFTSMSGVDY
                     ETHRPEMICFVPSIDVLKFWEKDYLLMQENMIYGDSLSFSALIDRVKELNKRFNSTKF
                     "
BASE COUNT         1219 a          646 c          648 g         1180 t
ORIGIN      
        1 aaacgctatg ccgaacaccc agattatcaa acttcacgct acggcgaaca gggggttgtg
       61 ttcattcttt tttcaacaac caatctacaa tgtttatatg ttctattccc tcaatttcag
      121 cagttattat atcagtagag agaatgtatt ttgggtaatt gtctgaaata gattttaacg
      181 gacgaatttc tcgctcagcc gtttttgggt cagttattga ccatgctacc tgataatact
      241 caaacttatt gagtgcgttt tttacgataa aatccacttc atattctcca atttttccaa
      301 tccaaatttg ataacctcgc ctgtttaact ccaagtacac aatattctca agaatatgtc
      361 ctacatcttg atattggaat tttcccagtt tcaaattccg aaatccaata tctaccaaat
      421 agtatttttc ttgtgtagca agttgctgtt tcccttttat atcaaagcga tttacttgat
      481 aaaaaacgta agcacttact aaatactcaa tatatttttc cacggtattg tggtgaatag
      541 cttgacggtc ttgtttcatt gcttttgaaa tattgtttgg cgatactaca cttccgattg
      601 cacccataag aaattttgat aggttgtcaa aacttctact attctgtata gtataccgct
      661 gtctaatatc tttttctaca atggtgttat acacatcttg aatgtacata ttttggatat
      721 ccgtaccttg attgcgtaaa aaaacgccct gcggcaaaga ggtttcataa agatagtggt
      781 taaataactc gtacttattc attgtcgtat ctttctgact ttcgcaaaat tctgcaaacg
      841 aaaatggcaa gatgtgaata gttatgtagc gtccagaaag taaggttgct agatcgcccg
      901 acaaaaaata agcatttgag cctgttacat ataaatcaat atatcgttca acataaagag
      961 catctataag gcgttcaaat tcagaaatct gttgtggttc atcaagaaaa atataattca
     1021 tattatcctt gtctgttttt gctaaaatgt ccttatagat atctcgccaa gtgtattctt
     1081 ggggataaat tggcttttca aaattatatt gctgaacgtt tcttgcagag acaccagaat
     1141 ctcgtatctg ttgaataaaa atctcaaaca aggttgactt acccgaacgt ctcaatcctg
     1201 taataacctt gatgatatgc ttatctttca acttctctaa ctgctgaatg taattttgtc
     1261 ggtttattaa atgattcatc tttcatttgt ttttaatcat ccgcaattca ctttatcaaa
     1321 acttctaatt attgaaagtt ttgataaaga tactactttt tacacgaata acaagaaacc
     1381 gttgacaaat tattttacaa attactggta taattctatg tatatttact ttgctttgta
     1441 aaacctgcca tttgtttgca ctcgctacca cacgattgct ctacacgttt gccataggat
     1501 ataacctcgc actacacgct aagtgcatgg cgtttctttt ctgatgcttt tgttgttcag
     1561 gcaaggcaca gccttgattt tggttcgaat agggaaagct ggagctttcc ttttaacttc
     1621 gacatagcga gacgctatgc cgaacaccag attgtcaaaa ttcacactac ggcgaacaaa
     1681 ggtgcgtaaa ttacaagaca tttacaattt cgagttagct attgttttat tcacaaatat
     1741 agattacttt tgtgaaatat aaaactttgt atttatggca caatcaattg aaaataaggt
     1801 attagaacat ctaaagtata gacagagagg aaagatatat ttcaccaacg attttgcaac
     1861 tcttggaact tcagaatcta ttcgtaaaag tttatcgaat cttgtaaaaa aagatatact
     1921 tatcagacta actcagggaa tttatctata cccaaaaata gacaaagatc ttggagtgct
     1981 atatccatct gttgacgacg tgtgtagagc tattgcaaaa cgtgacaaag caagaattga
     2041 acctaccggg atatttgcat tacacagttt gggactttca acacaaatac cggtaaacgt
     2101 ggtttacctg actgacggaa ttccacgaaa aataaagtac ggaaaccgta caataaaatt
     2161 caaaaaaact gcgccaaaaa atcttgccat gaaaggtaaa ataagcagtt tggttattgc
     2221 tgcattaaag caaattggaa aagaaaatgt atcacaagaa gttgaacttc aattaacaaa
     2281 ggcattaaag aaagaatctg ttgaaaatgt aaaacatgat gcacagttag ctcccgtatg
     2341 gatttcgaaa ttaatatttc aactcgtaaa taacattgaa aaatgaattg gacagaatta
     2401 tctacaacca gacgggttga agttttaaaa catcttgaaa acaaaacagg actaaatgct
     2461 acttctatcg aaaaagattg gtgggttact ttggcattga atgcattgtt taacggactt
     2521 tatggttcgc atttagtttt taaaggagga acttcgctta gtaaatgttg gcgaattatc
     2581 gaccgttttt ctgaagacat tgatttggca attgacagag agttccttgg tttttcgggc
     2641 aaactgacaa aacgagaaat tggaaagaaa cttagaaaag catcgtgtgc atttatacga
     2701 aatgagctaa aggcggatat tgaacgattt attctgaatt gtggtattaa caacgaccaa
     2761 tttaaactag aagtaaatat tacaactgaa agtaacgtag accctgaaac gctttattta
     2821 cattacgaat ctgtattgga taattccaat tacgttttca ataccgtaaa aatcgaagtt
     2881 ggttcacgtt cgctaatgga accaagtgag gaggtagcaa ttcaatcaat aattggagaa
     2941 cattttccta aagttgattt ttcggacaag gagtttaaag taaaagccgt attgccacaa
     3001 agaaccatgc ttgaaaaagc atttctttta cacgaaatgt tacaaagtcc aagttctgga
     3061 agagaaatta acagaatgtc gagacatttg tacgacttag aaaaattgat ggatagtgca
     3121 ttttgcgacg atgcgttaca aaacagagaa ttgtattacg acatactaaa tcatcgcaaa
     3181 ctgttcactt caatgtcagg agttgattat gaaacacatc gaccagagat gatttgtttt
     3241 gtaccttcta tagatgtact gaaattctgg gagaaagact atttgctaat gcaggaaaat
     3301 atgatttacg gtgattctct cagtttttcg gcattaattg acagagtcaa agaattgaac
     3361 aaaaggttta attcaacaaa attctgaaac tcgacagagt gtttagtgtt tagcgtttaa
     3421 tgtttagtgt tgttcgttta acgttgtttg tttttcgtgc agaattaaca actatcaatt
     3481 ttcaactttc aactctccac agtacattcg tttgagagct ttgcttttag taggcacagc
     3541 ctacgatgaa ctaggggtaa ctccgcccag caatagcaat atgtaataca ctaccgacag
     3601 ctaggggcag aagatgccat tcggcacgaa aatcccaagg caaagccatt gggttgatac
     3661 tacgtatggc tttcagccaa ccacaacgcc ctg
//
LOCUS       DAOSMY010000142         3685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25145_length_3685_cov_2.550964, whole genome shotgun sequence.
ACCESSION   DAOSMY010000142 DAOSMY010000000
VERSION     DAOSMY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25145_length_3685_cov_2.550964"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PLD76_04140"
     CDS             complement(<1..1419)
                     /locus_tag="PLD76_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOU27227.1"
                     /translation="MNFEKFTIKSQEAIQKAIELAQTNGQQAIETAHLLKAILTVADA
                     VSQFIVQKLNINIAQLNQSVDGLIRSLPKVSGGQPYVSNETNKVLQTAQSLSEKAGNQ
                     FVSVEYIVLALVKEKSNISQLFKELGVNEQNVEAAISELTKNQKVTSPSAEDTYQALS
                     KYAVNLNEKARSGKLDPVIGRDEEIRRVLQILTRRTKNNPILIGEPGTGKTAIAEGLA
                     HRIVRGDVPENLKSKQLYSLDMGALIAGAKYKGEFEERLKSVVNEVVNADGEIILFID
                     EIHTLVGAGKSDGAMDAANILKPALARGELRSIGATTLDEYQKYFEKDKALERRFQIV
                     MVNEPDEASTISILRGLKERYENHHKVRIKDDAIIAAVELSNRYISDRFLPDKAIDLI
                     DEAAAKLRLEVDSVPEELDKIERTIKQLEIEREAIKRENNEQKLTQLSTEIANLKEEE
                     KEKKAKWKAEKELIESIQQNKID"
     gene            complement(1668..1952)
                     /locus_tag="PLD76_04145"
     CDS             complement(1668..1952)
                     /locus_tag="PLD76_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU27228.1"
                     /translation="MTDYLHRIAEKEINETLLAMGAVLIQGARAVGKSTIAKKLAKSD
                     ISLDSSVALIELAKISPNVILTGQTPRFIDEWQLAPSIWNAVRHFVAQVS"
     gene            2200..2466
                     /locus_tag="PLD76_04150"
     CDS             2200..2466
                     /locus_tag="PLD76_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27229.1"
                     /translation="MIHYVPYLIAFHIITNDILVAMAYTPQPCFQRGVNGELFSTSLF
                     VLCTLRSIYYFEMLTISLVENNVLLLECIENAITKIPSLKARGR"
     gene            complement(2492..3571)
                     /locus_tag="PLD76_04155"
     CDS             complement(2492..3571)
                     /locus_tag="PLD76_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOU27230.1"
                     /translation="MKKIELVEVYIYNELVGKIALTDKGLCAFQYSTTFLQKGFSISP
                     FELPLKPGVFVAKLHPFSGNFGVFDDCLPDGWGMIILERYLQKIGVRVRMLSLLDLLS
                     IVGSSGRGALEFRPDQSIHTSQELQDFSYISNEAQKILRFSEYNAESIDDLYRRGGSP
                     GGARPKIFVMVDGKEWLVKFGAINDPIHIGEIEYTYSLLAKKCAIEMPETRLFDGKYF
                     GVQRFDRVQSDKIHVVSAAGLLNADYRTPCLDYTTLFQLCQALTRNAQEMWKLYRLMV
                     FNYVIGNKDDHAKNFSFLYQNNEWKLSPAFDILPSEGINGFHTTTINNTLYPTKQDII
                     NVAVKAGLNKQKALYIFDEIVATVG"
BASE COUNT         1087 a          697 c          584 g         1317 t
ORIGIN      
        1 atcaatctta ttctgttgaa tagattcgat aagctctttt tcggctttcc atttagcttt
       61 tttttctttt tcttcttctt tcaggttcgc tatttcggtg gataattgtg tgagtttttg
      121 ttcattgttt tcacgtttaa tagcctcacg ttcaatttct aattgcttga ttgttcgctc
      181 aattttatct aattcttcgg gtacagaatc tacctccaaa cgcaattttg cagcagcttc
      241 atcaattaaa tcaatggctt tgtcgggcaa aaagcgatcg ctaatatacc tattagatag
      301 ctcaacagca gcaatgatgg catcatcttt gatacgtact ttatgatgat tttcataacg
      361 ctcttttaat ccacgtaaaa ttgatatggt actagcttca tctggttcgt ttaccattac
      421 tatctgaaaa cggcgttcta atgccttatc tttttcaaaa tatttttggt attcatctag
      481 cgttgtagca cctatagagc gtaattctcc tcttgccaaa gcgggtttta gaatattggc
      541 agcatccatt gctccatcac ttttacctgc accaaccaag gtgtgaattt catcaataaa
      601 tagaataatc tctccatcgg catttactac ctcattaacc accgatttta aacgttcctc
      661 aaattctcct ttgtatttag ctccagcaat aagagctccc atatccaaag aatagagttg
      721 tttggacttt aagttttcag gcacatcgcc tcttactatg cggtgagcta aaccttcggc
      781 tatggcagtt ttacccgttc caggttctcc aattaaaatt gggttgtttt ttgttctacg
      841 ggtaagaatt tgcaatactc ggcgtatttc ttcatctcta ccaattactg ggtcaagttt
      901 acctgaacgt gccttttcat ttaagtttac tgcatactta gaaagagcct gataggtatc
      961 ttcggcagaa ggtgaagtta ctttttggtt tttagtcaat tcacttatgg ctgcttccac
     1021 attttgttcg tttacaccca attctttaaa aagctgagaa atattggatt tttctttcac
     1081 cagcgccaag acaatgtact ctaccgaaac aaattgattt ccagcttttt cggataatga
     1141 ttgagctgtt tgaagtactt tgttggtttc gttgcttaca tatggttgcc ctccagaaac
     1201 tttgggtaac gagcgtataa gaccatcaac agactgattg agttgtgcga tgttgatatt
     1261 cagcttttga acaataaatt ggcttacagc atctgcaaca gtcagtatag ctttgagtaa
     1321 atgagctgtt tcaatggctt gttgaccatt tgtttgagct aattcaatgg ctttttgaat
     1381 agcttcttgc gatttgatag taaatttttc gaaattcata ggtatatcct ccttttttac
     1441 tatcaacctt caaaaagttt gccgagaata tataactgac aatttgacag aacaaatata
     1501 cattattaaa cgttgataag ggtttattta tagctaattt ttcattataa atagttgtat
     1561 ttcaatattt tagacaaaga tatataccta aatacactta caccagttgc acacaataat
     1621 atctttatag ctaacgagga gaagagagat tgaaacgaag gcacaagtta tgaaacttgc
     1681 gccacaaagt gtcgtactgc gttccaaatc gaaggagcta attgccactc gtcgataaaa
     1741 cggggtgttt gcccagttaa aatcacattg ggactaatct ttgcaagctc aatcaaggct
     1801 actgaagagt cgagtgaaat gtcacttttt gctaactttt ttgcaatggt agatttacct
     1861 acagcacgtg ctccttgtat aagtacagca cccattgcca ataaggtttc atttatctcc
     1921 ttttcggcta tcctgtgtaa ataatcagtc atgaaatatc attattatat tcaacttaat
     1981 atcactttta cgttcatatt aatatcactt ttacgtttac aaagatattg attttgcatc
     2041 agattatatc attattgcct gaaaataatt cacaacatta tttatctatt atgattatgt
     2101 ctcatcacaa ctctcacaat tactgcaatt gtgctttatt aaatttgtaa ttacatacta
     2161 ttgaattgct ttatatttgt acataagaat ctattactta tgattcatta tgtaccttac
     2221 ttaattgctt ttcatattat aacaaatgat atcctagttg ctatggctta tactcctcaa
     2281 ccctgctttc aacgaggagt taatggtgaa ttattttcaa catcgctttt tgtgttatgc
     2341 actttgcgaa gtatttacta ttttgaaatg ctcactatct cgctcgttga aaacaatgta
     2401 ttattattgg aatgcattga aaacgctata acaaaaatcc cctcgttgaa agcgagggga
     2461 agataaagga aaggaagagc ttttttttaa gctatcccac agtggctact atttcatcaa
     2521 atatatatag ggctttctgc ttatttaacc ctgcttttac tgccacatta atgatatctt
     2581 gttttgttgg atataatgta ttgttaattg ttgtggtatg aaatccattt attccttcac
     2641 ttggtaagat atcaaatgca ggagacaatt tccattcatt attttggtat aaaaatgaaa
     2701 agttttttgc atgatcatct ttattcccta taacataatt gaataccatt aatcggtaca
     2761 atttccacat ttcttgagca ttacgggtca aagcctgaca tagttggaaa agggttgtat
     2821 aatctaaaca tggtgtacga tagtcagcat tcagcaatcc ggcagcactt accacatgta
     2881 ttttatcgga ttgtactcta tcaaatcgtt gaactccaaa atattttcca tcgaaaagac
     2941 gcgtttctgg catttctata gcacattttt tggctaatag ggaatacgta tattcaattt
     3001 ctccaatatg tatggggtcg tttatagcac caaatttaac gagccattct ttaccatcta
     3061 ccattacaaa tatttttgga cgtgcaccac ccggcgaacc tcctctacga tacaaatcat
     3121 caatagattc tgcattatat tcagagaatc tgagtatttt ctgggcttcg tttgatatat
     3181 atgagaaatc ttgtaactct tgtgaagtat gaatactttg gtcgggacga aattctaatg
     3241 cccccctacc cgaactacca actatagata gaaggtcgag caaagacaac atacgcactc
     3301 ttactccaat tttttgcaaa tatcgttcaa gaataatcat cccccaacca tcgggcaaac
     3361 aatcatcaaa aacgccaaaa tttccgctaa atgggtgcaa ttttgcaaca aatacacctg
     3421 gctttaatgg caattcaaat ggtgagatag aaaatccttt ttgtaaaaaa gtagtactat
     3481 actgaaatgc acacagtcct ttatcagtaa gtgcaatttt accaactaat tcattgtaga
     3541 tatatacttc aaccaattca atttttttca tatcattcca tatcttggtt tcttatagta
     3601 tagtacctct ttttcttgaa cttgatccag cttttaatac atcttcaata cgttggtatt
     3661 tttgttgaga gaaaagacta gtgaa
//
LOCUS       DAOSMY010000143         3683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25158_length_3683_cov_2.883131, whole genome shotgun sequence.
ACCESSION   DAOSMY010000143 DAOSMY010000000
VERSION     DAOSMY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25158_length_3683_cov_2.883131"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLD76_04160"
     CDS             <1..664
                     /locus_tag="PLD76_04160"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOU27231.1"
                     /translation="VKLLKLKYYIFAIFVFSCLSLWASDDSLAVVSKSDLVQEKEVVL
                     YVDSTLLHAEFKPQPSKAIWYAAVVPGLGQIYNRKYWKLPILYGGAMGLTYAISWNGG
                     MYRDYQKAYRDIVDNDASTQSYVNILPQGVVIDSDNKAYYTSLLKNKQDNYLRYRDLS
                     IIGTVGLYLLSIVDAYVDAHLYDFDVSPDLSLKIVPKAIPLKHINDFSDVSFGLSCQF
                     SF"
     gene            687..2096
                     /locus_tag="PLD76_04165"
     CDS             687..2096
                     /locus_tag="PLD76_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU27232.1"
                     /translation="MFINKIISSLFVIWLVFVTPSYASSIYPSQSSPLLVSEYESSMQ
                     TFDTAIEEMCVAWRMRIKTKETCDSAFRYASLPYSPDSIRIKRIAMMPTILEMTYNDV
                     VGRYINLYCDRNREISYMLALSQHYFPIFETIFEQYNLPNELKYLSIVESALNPRATS
                     RMGAAGLWQFMPTTGKLYNLEMNSIYDERRDPIKSTHAAAQHFRDLYEIFQDWNLVIA
                     AYNCGTGNVNKAIRRSGGKKDYWEIYPYLPIETRNYVPAFIAVNYMMYYADSYHICQA
                     SIDIPLVTDTLHVTKRIDLLHVSQVLDIPIEQIRLLNPCYKNDIIPDSEKPFILRLPS
                     HLAYLFIEKQDSLYMSQRDVTKQPISLSASATPHACSSVHIVRSGENLSIIAKRYGIT
                     VRQLMNWNNLTSPLVRVGQRLHLNSSKDISVYVVKKGDSLWTIAKNTSVSVEKIKQAN
                     RNKNLRILQPGQQLTIPKN"
     gene            complement(2148..3362)
                     /locus_tag="PLD76_04170"
     CDS             complement(2148..3362)
                     /locus_tag="PLD76_04170"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOU27233.1"
                     /translation="MTTFKLNSIEEAIEDFKEGKFLIVVDDEDRENEGDFIIAAEKIT
                     PEAVNFMLINGRGVLCAPITEERCAELELEKQVSNNTSILGTPFTVTIDKIEGCSTGV
                     SAADRAATIRALADPTSTPNTFGKPGHISPLYAKTKGVLRRAGHTEASIDLARLAGLY
                     PAAALIEIMNEDGTMARMPDLQKVAEKFSIKIITIKDLIAYKIKQESIVEKGVTVHMP
                     TQYGMFKLTPFRQKSNGLEHIALTKGEWKDDEPVLVRVHSSCATGDIFGSLRCECGEQ
                     LHHSMKMIEKEGKGAIVYLYQEGRGIGLMNKIKAYALQDNGMDTVDANLHLGFKADER
                     DYGVGASILREIGVRKMKLLTNNPVKRIGLESYGLEIVENIQIVVGINKYNETYMKTK
                     QERMGHNIISSK"
     gene            complement(3373..>3683)
                     /locus_tag="PLD76_04175"
     CDS             complement(3373..>3683)
                     /locus_tag="PLD76_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU27234.1"
                     /translation="IGAPLGAIIRKGGMGLPIVISIVLFIVYYIIDNTGYKMAREGIW
                     EVGMGMWFSALVLLPLGMFLTYKAVTDAVILTTEGKLFNPKKWINALIALVKKKKKNI
                     "
BASE COUNT         1129 a          643 c          639 g         1272 t
ORIGIN      
        1 agttaaactt ttgaaactga aatattacat atttgctata ttcgtattta gttgtctatc
       61 tttgtgggca agtgatgatt ctttggctgt agtatcaaaa tcagacttag tacaagaaaa
      121 agaggtagtg ttatatgttg attcaacact tttacacgct gagtttaaac cccaaccttc
      181 aaaagctatt tggtatgcag ctgtagttcc tgggctagga cagatataca acagaaaata
      241 ttggaaactg cctatattgt atggaggtgc aatgggttta acgtatgcta tttcgtggaa
      301 tggaggaatg tatagggatt atcaaaaagc atatagagat attgttgata atgatgctag
      361 tacccaaagt tatgtaaaca tactacctca gggagtagtg atagatagtg ataacaaagc
      421 atactatacc agtttgttga aaaataaaca ggataattat ttaagataca gggatttgag
      481 tattattgga acggtcggtc tatatctttt aagtatagta gatgcgtatg ttgatgctca
      541 tttatatgat tttgatgtta gtccagattt atctttaaaa atagtgccta aagccattcc
      601 attgaaacat atcaatgatt ttagtgatgt ttcttttgga cttagttgtc aattttcttt
      661 ttgataatta agaggtagta gaaagaatgt ttataaataa aattatatcc agtttgttcg
      721 ttatatggtt ggtgtttgtt acaccaagct atgcctcttc tatttatcca tcgcagagct
      781 ctccgttact tgtttctgag tatgaatcat ctatgcaaac gttcgataca gcaatagaag
      841 agatgtgtgt tgcatggcgt atgcgaatta aaacaaaaga aacatgtgat agtgcattcc
      901 gttacgcttc attaccttat tcgcctgatt caattcgtat taaacggatt gcaatgatgc
      961 ccacaatttt ggaaatgacg tataatgatg tagtaggtcg ttatatcaat ttatattgcg
     1021 acagaaacag agaaatatct tatatgcttg cacttagtca gcattatttc ccaatttttg
     1081 aaactatttt tgagcagtac aatcttccca atgaactaaa gtatttgtct atagtagaat
     1141 cagcactgaa tcctagagct acttcgcgta tgggagccgc agggttatgg caatttatgc
     1201 caactacagg taagttatat aacttagaaa tgaatagtat atatgatgag cgaagagacc
     1261 ccatcaaatc aactcatgca gctgcccagc attttagaga tttatatgaa atatttcagg
     1321 attggaattt agtgatagcc gcctataatt gtggaactgg aaatgttaat aaagctatca
     1381 gaagatcggg tggaaaaaaa gattattggg agatttatcc atatttacct attgaaacac
     1441 gtaattatgt gcctgcattt attgcagtga attatatgat gtattatgca gatagctatc
     1501 atatttgtca agctagcatt gatattccat tggtaacaga tactcttcat gttacaaaaa
     1561 ggatagattt gttacatgtt tctcaggtgt tggatatacc gattgagcaa atacgtttac
     1621 ttaatccctg ttataaaaat gatataattc ctgattcaga aaaacctttc attttacgac
     1681 ttccttctca tttagcgtat ttatttatag aaaagcaaga ctctttatat atgtcgcaac
     1741 gtgatgtgac taaacaacca atttctctat cagcatcagc tactcctcac gcatgttcat
     1801 ctgtacacat tgtacgttct ggagaaaatt tgtcaattat tgccaaacgt tatggtataa
     1861 ccgtccgtca attaatgaac tggaataatt taacttcacc attagttcgt gtagggcaac
     1921 gtttacatct caattcgtct aaagatatat ctgtctatgt ggtaaaaaaa ggagatagct
     1981 tatggactat tgccaaaaat acaagtgtct cggtagagaa gataaaacaa gctaatagaa
     2041 ataaaaattt gcgtattttg caaccaggtc agcaactgac tattccaaaa aattaaagga
     2101 accaattgtg tagtttgatt cctttgtatt gttttagtta tggagggcta ttttgacgaa
     2161 atgatattat gtcccattct ttcttgtttc gttttcatat aggtttcatt gtatttatta
     2221 atacccacca caatttgaat attttccaca atctctaatc cgtaagattc taacccaatt
     2281 ctttttacag gattattcgt aagaagtttc atttttctca cacctatttc tcttaggata
     2341 ctagcaccta caccataatc tctttcgtca gctttaaagc caagatgtaa attagcatct
     2401 acagtatcca tcccattgtc ttgtagggca tacgctttaa tcttgttcat taaaccaata
     2461 cctcttcctt cttgataaag atatacgatt gcaccttttc cttctttttc aatcattttc
     2521 atactatgat gtagttgttc accacattcg caacgcaatg agccaaaaat atctcccgta
     2581 gcacacgatg agtgaacacg tactaaaaca ggctcatcgt ctttccattc tcctttggtt
     2641 aatgcaatgt gctctaatcc atttgatttt tggcgaaacg gagttagttt aaacattcca
     2701 tattgagtag gcatatgtac agttacaccc ttttcaacaa ttgattcttg tttaatttta
     2761 taggcgatta aatctttaat tgtaattatt tttatactaa atttctctgc tactttttgc
     2821 aaatctggca tacgtgccat tgtgccatct tcgttcatga tttcgattag agctgcagca
     2881 ggatacaagc ctgccaaacg agctaaatca atagatgctt cggtgtgtcc agcacgtctg
     2941 agtacgcctt tggtttttgc gtatagcgga ctaatatgtc caggtttgcc aaatgtattt
     3001 ggagtagaag taggatcggc aagagccctg attgtagcgg ctctgtcagc agcagacact
     3061 cccgtcgaac aaccttcaat tttatctatg gtaacggtaa aaggagttcc aagtattgag
     3121 gtattgtttg aaacttgctt ttctaattca agctcggcac aacgttcttc tgtgatagga
     3181 gcacacaaca caccacgacc attaattaac ataaaattta cagcttctgg tgtaattttt
     3241 tcggcagcta taatgaagtc tccttcattt tctctgtctt catcgtctac tacaattagg
     3301 aatttacctt ctttgaaatc ttctattgct tcttcaatag aatttaattt aaacgtagtc
     3361 atattttttt agttaaatat ttttcttttt tttcttgact aatgcaatta gagcgtttat
     3421 ccattttttt ggattaaata gtttgccttc ggtggtaaga ataacagcgt cagtaactgc
     3481 tttataggtt aagaacatac ccaaggggag caataccaat gcactaaacc acattcccat
     3541 acctacttcc catatacctt cacgagccat tttgtatcca gtattatcaa ttatgtaata
     3601 tacaatgaat agcacaattg aaataacgat aggcaaaccc attcctcctt tgcggatgat
     3661 ggctcctaat ggtgctccta taa
//
LOCUS       DAOSMY010000144         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25166_length_3682_cov_3.969948, whole genome shotgun sequence.
ACCESSION   DAOSMY010000144 DAOSMY010000000
VERSION     DAOSMY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25166_length_3682_cov_3.969948"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PLD76_04180"
     CDS             complement(<1..1642)
                     /locus_tag="PLD76_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU27235.1"
                     /translation="MNNLLVYQASAGSGKTFKLTENYLRLLFSEPDAYKRTLAVTFTN
                     KATEEMKTRILLELHKLADNQESDYIPIFCDRNNPFDVLALRKKAKEILQTILHDYSR
                     FKVSTIDRFFQQIIRSFIREIGLNNNYILEVDNKKILIETVETMMFQLEDKKHKKLLE
                     WLISLAEQRIESGQNWSFKNEIVTLGTEIFKEHYLRFSSELEKVTEKEFLHDYITSLK
                     NLKKTVKQTLQQKATHILDIIANNGLSTADFKRYTLDALEHFAKNDIRELTATFLKLE
                     SDTTAYCTKNSPNKDRINSLVSNGLHELITDFIRTHQSIILQYNTAEVIIKYSNILGI
                     ITDIHTCIKENNNENNALLLSDSSNLLHSIINDSDTPFIYEKIGTYIDHYLLDEFQDT
                     SRLQWENFKPLISNSLANGNTNLIVGDVKQSIYRWRSSDWKILAQQLDKEFPQYVQHE
                     SLLDNWRSDEEIISFNNKLFLFATQQLQAKYVDDIAKAGIDTTNNSLTHLLTESYAQV
                     QQNFPKKKEYRKNKGYIEIQFTDRNNKEEFEAYTLLQMV"
     gene            complement(1659..2018)
                     /locus_tag="PLD76_04185"
     CDS             complement(1659..2018)
                     /locus_tag="PLD76_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOU27236.1"
                     /translation="MKNNDWKTKLGIVFSTNPEFGQTEEEEEPTLPIHSVNPSKQTLR
                     IEIDKKGRNGKCATLITGFIGDDVALKELAKKMKTSCGVGGSTRDGEILIQGDVRTKI
                     DELLRSEGYKTKLITGK"
     gene            2132..2746
                     /locus_tag="PLD76_04190"
     CDS             2132..2746
                     /locus_tag="PLD76_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU27237.1"
                     /translation="MKILAVGWNYAEHNQELGQTLPTEPIIFSKPDSALLLGNKPFFM
                     PNFSNEIHYETELVVRINRLGKNIAPRFASRYYSEVALGIDFTARDLQRSLKAAGQPW
                     EISKAFDNSAAVSEFIPLSSLSVSIQELSFYLQLNDKIVQQGNTKDMIFSVDTIIAYV
                     SQFFTLKIGDLIFTGTPAGVGQIAKNDHLQGYLQGNRLLNFYIK"
     gene            2757..>3682
                     /locus_tag="PLD76_04195"
     CDS             2757..>3682
                     /locus_tag="PLD76_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27238.1"
                     /translation="MKLYSFFLVFLLFSTLSAMSQQEHRFSIAIDWKKVQDLRANDEV
                     QRRLFFEGANYLGENQLPYFTYTIPISKEGTLSTYTVALDNAVFTSSTDDETTVLSQL
                     GVPVQTTIHIDYCIVYEKKQPSLQLLFTPIIQKENSYAKLIKADLIISSTPANITGNQ
                     GKRSLGDEDQSIHQYASSSVLASGNWYQIRVPQTGIYKLTYEDLLSLGIQNPAQVRVF
                     GYGGAILEEDFSIPRVADDLPEIALFMEKGSDDVFNAGDYILFHAQGTIRWQYDKYSG
                     YYKHQQNYYASHGYYFVTERAGEGKRITSQPLV"
BASE COUNT         1110 a          594 c          621 g         1357 t
ORIGIN      
        1 taaccatttg aagcaatgta taagcctcga attcttcttt gttgtttcta tctgtaaact
       61 gaatttctat ataccctttg tttttccgat attctttttt cttaggaaaa ttttgttgta
      121 cttgagcgta tgattctgtc aacaaatgtg ttaatgaatt gtttgttgta tcgatgccag
      181 cttttgctat atcgtctaca tattttgctt gtaactgctg tgtggcaaat aaaaaaagct
      241 tattattgaa agaaataatt tcttcgtcgc ttcgccaatt atctagcaaa gattcatgtt
      301 gaacgtattg tggaaattct ttgtcaagtt gttgagccaa tattttccaa tcagaactac
      361 gccaacgata tatactttgt tttacatcac ctactattaa gttcgtgttc ccatttgcta
      421 aactattaga tataagtggt ttaaagtttt cccattgcag tcttgaagtg tcctgaaatt
      481 catctagtag gtagtgatca atatatgtgc caattttttc gtaaataaaa ggggtatcac
      541 tatcattgat gatgctatgt aatagattgg aactatcgct taatagtaaa gcattatttt
      601 cgttattatt ttctttaata catgtgtgga tatcggtaat aattcctaaa atattactgt
      661 attttattat aacttcggcg gtattgtatt gcaatataat agactgatgc gtgcggataa
      721 aatccgtgat aagttcatgc aacccattgc ttactaatga attaattcta tccttgtttg
      781 ggctattttt tgtgcaataa gcagtagtat cgctttcgag ttttaaaaaa gtggctgtta
      841 attcgcggat gtcatttttt gcaaaatgtt ctaatgcatc cagtgtgtat cttttgaaat
      901 cagctgtgct aagtccatta tttgcaataa tatccaaaat atgtgtagcc ttttgttgta
      961 gcgtttgttt aacagttttt tttagatttt ttaaagaggt aatatagtca tgtaaaaatt
     1021 ctttttcagt aactttttct aattcggaac taaaacgcaa gtagtgttct ttaaatattt
     1081 ctgtacctag tgttactatt tcattcttaa atgaccagtt ttgaccactt tcaatgcgtt
     1141 gttctgctaa agagataagc cattcgagta attttttatg ttttttatct tctaattgaa
     1201 acatcatcgt ttcgactgtt tcaattagaa tttttttgtt gtcgacttct agtatatagt
     1261 tgttatttaa tccaatttcg cgaataaagg agcgaataat ttgctgaaaa aaacgatcaa
     1321 tggtacttac cttaaaccgt gaataatcat gtagtattgt ttgtaatatt tccttagctt
     1381 tttttcgtaa agccaataca tcaaaagggt tattcctatc gcaaaaaata ggaatataat
     1441 cactttcttg attgtcagcc aatttgtgta attctagcaa aatacgtgtt ttcatctcct
     1501 ctgttgcctt gttggtaaag gtaacagcca gtgttcgttt gtaggcatct ggctcactaa
     1561 aaagtaaccg tagatagttt tcagtaagtt taaatgtttt tcctgaacct gccgaagctt
     1621 gataaaccag taaattattc atcccttttt tttgctaatt attttcctgt aataagtttt
     1681 gttttatacc cttctgaacg taggagttca tcaattttgg tacgcacatc tccctgaatt
     1741 aatatttctc catcacgtgt tgaacctcca acaccacaac ttgttttcat cttttttgcc
     1801 aattctttta gggcaacatc atcgccaata aaacctgtaa ttaatgtggc acattttccg
     1861 tttctccctt ttttgtcaat ttctatacgc aatgtttgtt tggatgggtt tactgaatga
     1921 attggtagag taggttcttc ttcttcttct gtttgcccaa attcaggatt tgtagaaaat
     1981 acaataccca atttagtttt ccaatcgttg tttttcatat attttaaatt ttattacaaa
     2041 agaaacaaaa ctaactgaat aaaaaaaata ttacctttgt aaaatatcca caattagtac
     2101 aacggatatt tattagaaaa aaaacagata aatgaaaata cttgcagtag gatggaatta
     2161 tgcggagcat aatcaagagt taggccaaac tttacctacc gaacctatta tatttagcaa
     2221 acctgattct gcattgttgt tggggaataa accttttttt atgcccaatt tctccaatga
     2281 aattcattat gaaacagaat tagtggtacg aattaatcgt ttaggaaaaa atatagcacc
     2341 acgatttgct tcgcgttatt atagcgaagt agctttaggt attgatttta ctgctcgtga
     2401 tttgcaacgt tcattaaaag cggctggtca accttgggaa attagcaaag cgtttgacaa
     2461 ttcggctgcg gtaagcgaat ttattccatt aagctcatta agtgtttcta tacaggagtt
     2521 atcattttat ttgcaactta atgacaaaat tgtgcaacaa gggaatacta aagacatgat
     2581 tttttccgtt gatactataa ttgcgtatgt gagtcaattt tttaccctta aaattggtga
     2641 tttaattttt acaggtacgc cagcgggagt aggtcaaatt gctaaaaatg atcatttgca
     2701 aggttatttg caaggtaacc gattgcttaa tttttatatc aaataaatat tcatccatga
     2761 aattatatag tttctttttg gtctttttgt tgttttcgac actatctgca atgtcgcaac
     2821 aagaacatcg tttttcaatt gcgatcgact ggaaaaaagt gcaagacttg cgggcgaatg
     2881 atgaggttca aagacgctta ttttttgaag gagctaatta tttgggggag aatcaattac
     2941 catattttac gtatacaatt cccattagca aagaaggaac actttctacc tataccgttg
     3001 cccttgataa tgctgttttt acttcttcta cggatgatga aacaactgtg ttatcccaat
     3061 tgggagtacc cgtacagaca acaatacaca ttgattattg cattgtttat gaaaaaaaac
     3121 aaccatcact tcaacttctt tttactccaa ttattcaaaa ggaaaattct tacgcaaaac
     3181 ttattaaagc agatttgatt atctcttcta ctcctgcaaa tataactggg aatcaaggaa
     3241 aacgatcatt aggtgatgaa gatcaatcta tccatcaata tgcatcgtca tctgtattgg
     3301 cttctggtaa ttggtatcag attcgtgtgc cacagactgg tatttataaa ttaacctatg
     3361 aggatttgtt gtcactaggt atacaaaatc cagctcaagt gcgtgttttt ggttatggtg
     3421 gagctatctt ggaagaagat ttttcaattc ctcgtgtagc tgacgatttg ccagaaattg
     3481 cactatttat ggaaaaagga tctgatgatg tgtttaatgc gggagattat attttgtttc
     3541 atgcacaagg tactatccgc tggcaatacg ataagtatag tgggtattat aaacatcaac
     3601 agaattatta tgcatcccat ggatattatt ttgtaacaga aagagcagga gaaggcaaac
     3661 gcataacatc tcagcctctt gt
//
LOCUS       DAOSMY010000145         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25170_length_3682_cov_2.617039, whole genome shotgun sequence.
ACCESSION   DAOSMY010000145 DAOSMY010000000
VERSION     DAOSMY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25170_length_3682_cov_2.617039"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLD76_04200"
     CDS             complement(<1..396)
                     /locus_tag="PLD76_04200"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU27239.1"
                     /translation="MSLLLDADIMYLPGMGPKRAQLFNNELSIFTVKDLIFHFPYKYI
                     DRSRFYNIHEIDTTLTYIQVRGKITGFQLLGEGRNQRLIGKFTDGRNVIDLVFFKGIK
                     YCTQAYQLHTEYIVFGKPSLFNGTFSFVHP"
     gene            complement(467..1153)
                     /locus_tag="PLD76_04205"
     CDS             complement(467..1153)
                     /locus_tag="PLD76_04205"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOU27240.1"
                     /translation="MSNIQAKYVVVVAGGKGKRMQSEMPKQFLLLHGKPILMHTLETF
                     FLYDHSIQIILVLPEDDIDMWKKLCQQHAFELAHTIVNGGNERFYSVANGLTLVPEGV
                     LVAIHDGVRPFPSTSTIERTFGMAAEKGNAIPVVELTDSIRQLSDNQENKSVSRAAFR
                     LVQTPQTFQSTQIKKAYRQPYSPLFTDDASVLESVGESIFLVEGNRENIKITTPLDLV
                     IGEYLLDQRK"
     gene            1268..1729
                     /locus_tag="PLD76_04210"
     CDS             1268..1729
                     /locus_tag="PLD76_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU27241.1"
                     /translation="MAKQVLFSILFGFLMAIQGMAQENPLENDSLLQEETILSEHDRM
                     DTILYRAKNTATSEIEFVSIGGFRVQVYAGNHPQKAREDAMLIDKTIKEQYPEYRAYV
                     SFQAPFWRVRIGDFASYYEALLLSRELKKIFPDFGNEIYVVKDPTVRILYF"
     gene            1733..2320
                     /gene="folE"
                     /locus_tag="PLD76_04215"
     CDS             1733..2320
                     /gene="folE"
                     /locus_tag="PLD76_04215"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU27242.1"
                     /translation="MEKEKTASSPEILQELASHYKAVIELIGENPTREGLLKTPERMA
                     KSMQFLMRGYNQDPEAIIRSAMFTEDYRQMVIVKDIDFYSMCEHHMLPFFGQAHVAYI
                     PNKKITGLSKIARVVDVFARRMQVQERLTTQIKECIQNTLNPLGVMVVIEAQHMCMQM
                     RGVEKQHSVTTTSDFTGIFEQAKTREEFISLISKK"
     gene            complement(2375..3127)
                     /gene="lptB"
                     /locus_tag="PLD76_04220"
     CDS             complement(2375..3127)
                     /gene="lptB"
                     /locus_tag="PLD76_04220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOU27243.1"
                     /translation="MLSENQMVLRTEHLVKRYKTRTVVDNVSISVKQGEIVGLLGPNG
                     AGKTTTFYMTVGLVTPNQGRIFLNDLDISSYPVYKRAQQGIGYLAQENSVFRKMSVED
                     NILSVLQMTKFSKKYQHEKLETLISEFSLQTVRKSLGDQLSGGERRRCEIARCLAIEP
                     KFIMLDEPFAGVDPIAVEDIQRIVAQLREKNIGILITDHNVHETLSITDRAYLLFEGK
                     ILFQGTAEELADNPVVREKYLGASFELRKKVE"
     gene            complement(3131..>3682)
                     /locus_tag="PLD76_04225"
     CDS             complement(3131..>3682)
                     /locus_tag="PLD76_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU27244.1"
                     /translation="EKPNLELAKVYVESGELFWNSGVFVWSVEAIWKAFNEFLPEMVA
                     KFEAGSDKFNTDLEQDFINEIFPSCQNISIDYGIMENAKNVYVLCGDFGWSDLGTWGS
                     LYDMSEKDEHQNVTLKCKTQFYESNDNIVVLPENKLAVIQNLSGYIVVESDNVLLICK
                     KEDEQRIRHFVTDVNLKYGEGYI"
BASE COUNT         1178 a          713 c          613 g         1178 t
ORIGIN      
        1 tggatgaacg aaagagaatg ttccattaaa taagctaggt ttaccaaata caatatattc
       61 agtatgtaat tggtatgctt gtgtgcaata ctttattcct ttaaagaaga ccaaatcaat
      121 tacatttcgc ccgtcagtaa actttcctat taaccgttgg tttcgtcctt ctcccaacaa
      181 ctgaaatcca gttattttgc ctcttacctg aatatatgta agggttgtgt ctatttcgtg
      241 aatattataa aatcggcttc tatcgatgta tttatacgga aagtggaata tcaaatcttt
      301 tacagtaaaa attgataatt cattattgaa tagctgagcc cttttcggtc ccatgccagg
      361 caaatacatt atgtctgcat ccaaaagtaa actcacttaa ttgggtttag tgtatgtacg
      421 agcaaatata gcaaaagaat ttaaaaatcg aagcctttca ctttatctat tttcgttgat
      481 ccagcaaata ctctccaata accaaatcta gtggagtagt tatttttatg ttttctctat
      541 ttccttctac caaaaaaatt gactctccta ctgattccaa gacacttgca tcatccgtaa
      601 ataagggtga atatggttgc ctataagcct ttttaatctg tgtagattga aatgtttgcg
      661 gagtttgcac tagacgaaat gcagcacgac taacggattt attttcctga ttgtcggata
      721 gttgacgaat agaatcagtt agttcaacaa caggaatagc attccccttt tcagccgcca
      781 taccaaatgt acgttcaata gtagatgtac taggaaaagg tcgtacgcca tcatgtatag
      841 ctaccaatac cccttcgggc actagcgtta acccgttggc aacggaataa aaacgctcat
      901 tccctccatt aacaattgta tgtgcgagtt caaatgcatg ttgttggcat aattttttcc
      961 acatatctat atcgtcttcg ggcaatacaa gaataatctg tatggaatga tcgtataaaa
     1021 aaaatgtttc taaggtatgc atcaaaattg gtttaccgtg caacaataaa aattgttttg
     1081 gcatctcact ttgcatacgt tttcctttac cgccagctac cacaacgaca tattttgctt
     1141 gtatgttcga cataaaaaat tgggtgttat aatttgtaca aaagtactat ttttctacaa
     1201 aaatacctgt aaaagccatt tttagaataa attttggtat ttttgcacat aaaataacga
     1261 aagaagtatg gcaaaacagg tattattcag tattcttttt ggttttttaa tggcaattca
     1321 aggaatggct caagaaaatc ctttggaaaa tgacagttta ttgcaagaag aaacaatact
     1381 atcggagcat gatcgtatgg ataccatttt gtatagagcc aaaaacacag caacttctga
     1441 gattgaattt gtaagtattg gtggatttcg cgtacaagtg tatgcgggca accatcctca
     1501 aaaagcacga gaagatgcta tgcttataga caaaactatc aaggaacaat atcctgaata
     1561 ccgtgcttat gtatcttttc aagccccctt ttggcgagta cgtataggag actttgcatc
     1621 gtactacgaa gcgttattat taagcagaga attaaaaaag atattccctg attttgggaa
     1681 cgaaatttat gtagttaaag accccactgt tcgtatttta tatttttaac gtatggaaaa
     1741 agaaaaaaca gctagttctc cagaaatatt gcaagaattg gcatcccatt ataaagctgt
     1801 aattgaactt attggagaaa accctacaag agaaggtttg ctaaaaacgc ccgaacgcat
     1861 ggcaaaaagt atgcagtttt tgatgcgtgg ttacaaccaa gacccagaag caattattcg
     1921 ttcggcaatg tttaccgaag attaccgcca aatggtaatt gtaaaagaca ttgattttta
     1981 ttcgatgtgc gaacaccaca tgttaccgtt ttttggacaa gcacatgtag cttatatccc
     2041 caacaagaaa attacaggat tgagtaaaat cgcccgcgta gtagatgtat ttgctcgtcg
     2101 tatgcaagta caagaacgat taactacaca aatcaaagag tgtattcaaa acactttaaa
     2161 tccgcttggc gtaatggttg taattgaggc tcaacatatg tgtatgcaaa tgcgtggagt
     2221 ggaaaagcaa cattcggtta caactacttc tgattttacg ggaatttttg aacaagctaa
     2281 aactagagaa gaatttatta gtttgataag caaaaaataa ttgatttgag catcgtaaaa
     2341 aaaataaagc agaccaaaag tctgctttat ttttttattc cacttttttt cgtaattcaa
     2401 aactggcacc caaatatttt tctcgcacta caggattatc tgctagctcc tcggctgttc
     2461 cctgaaaaag aatttttcct tcaaacaaca aatatgctct atctgtaata ctcaaggttt
     2521 cgtgcacatt atggtcggta attaaaatgc caatgttttt ttctctcaac tgtgctacta
     2581 tacgctgaat atcctccaca gcaataggat cgacacctgc aaatggctca tcaagcataa
     2641 taaacttagg ttcaatggct aaacaacgtg ctatttcgca cctacgacgc tcaccgccag
     2701 acagttggtc tcccaaactt ttgcgtactg tttgcaagct aaactcactg attaatgtct
     2761 ccaacttctc gtgttggtat tttttagaga attttgtcat ttgtaatacc gacaaaatat
     2821 tgtcttccac actcatcttt ctaaacactg aattttcttg agccaaatat ccaattcctt
     2881 gctgagctcg cttataaacc ggatacgaag atatatcgag atcgtttaga aaaatacgtc
     2941 cttgattagg tgttaccaag cctactgtca tataaaacgt agttgtttta ccggctccat
     3001 tcggaccaag caagccaaca atttcaccct gtttcactga gatggaaaca ttgtcaacaa
     3061 ctgtacgtgt tttgtacctt tttactaaat gctccgtgcg gagtaccatt tgattttctg
     3121 ataacataga ttaaatatat ccctcgccgt atttaagatt tacatctgtt acaaaatgac
     3181 gtatgcgttg ttcgtcttct tttttacaaa tcaacaacac attgtcagac tctaccacaa
     3241 tataaccaga aaggttttga ataaccgcta atttattttc gggcaatacc acaatattgt
     3301 cgttactttc gtaaaattga gttttgcatt taagtgttac attttgatgc tcatcttttt
     3361 ctgacatatc gtacaatgaa ccccatgtac caagatcaga ccacccaaaa tcaccacaca
     3421 atacatacac attcttggca ttttccataa taccatagtc aatagaaatg ttttgacacg
     3481 atgggaaaat ttcgttgata aaatcttgtt ctaagtctgt attaaattta tcgctacctg
     3541 cttcgaactt ggctaccatt tcgggtaaga actcgttgaa tgctttccaa attgcttcca
     3601 cactccatac aaatacacct gagttccaaa acaattcgcc actttcgaca taaacttttg
     3661 ctaattcaag gtttggcttt tc
//
LOCUS       DAOSMY010000146         3681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25182_length_3681_cov_3.072256, whole genome shotgun sequence.
ACCESSION   DAOSMY010000146 DAOSMY010000000
VERSION     DAOSMY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25182_length_3681_cov_3.072256"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLD76_04230"
     CDS             complement(<1..1322)
                     /locus_tag="PLD76_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU27245.1"
                     /translation="MEKPRLRFWQIWNMSFGFLGIQFGFALQNANVSRIFETLGADIG
                     NIPILWIAAPVTGLIIQPIIGHMSDKTWNKLGRRRPFFLAGAILASLALIFMPNSPYL
                     WVAAGMLWIMDASINISMEPFRAFVGDMLPAEQRTKGFAMQSFFIGTGAVIASALPYM
                     LTNWFGIANTAPEGLIPPSVKYSFYLGAIAFLVTVIWTVISTKEYSPEELATFEKNEQ
                     KENESTSNHHEAILPSSRFLKIAPIWTITGILLSVLVYFFSLEAELYILTGGITFFGL
                     ILFFVGGLVQANKQKNGLVEVINDLLLMPKTMKQLAIVQFFSWFALFAMWIYTTAAVT
                     SHIYGTSDTHSAIYNEGADWVGIMFAAYNGFAAIVAFLLPVLANYTNRRFTHVIALIA
                     GAVGLISIYFVENPTILLLPMLGVGLAWSSILSMPYAILTGSLPSHKMG"
     gene            complement(1348..3201)
                     /locus_tag="PLD76_04235"
     CDS             complement(1348..3201)
                     /locus_tag="PLD76_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU27246.1"
                     /translation="MKKTFLIVFLFVTLCLSGYAKSEKVTFERLEPASWWIGMHDTQL
                     QLMIYGKNISSSKVEFLYEGVSLVKRETTDNPNYLFLYLHIDKKTKPGTFDIVFKNGK
                     KQVGKFTYTLQARRENSAQRPSFSPADAIYLLMPDRFCNGNLQNDNVKGYIQGVNRDD
                     LGHRHGGDIEGITKKLPYLADLGVTALWITPFFDNNDKNYSYHHYATGDYYKVDPRLG
                     TKENYKELSTKCKENGLKLIIDVVPNHCGSAHWWMKDLPASDWLNKWDTYTSSNYRMT
                     AWTDPYSSEYDVKQLNKGWFSENMPDYNLANPHLFTYLKQAYIWWIEYADVDGIRVDT
                     YPYTDIRIGAKWIKAIRDEYPTINIVGECWVKTPQEIAFYQSGSMNKYGFDSHMQSVM
                     DFVLKDVFEFSFNERESWDRGMIRFYNHYAQDFLYADPNQIMNFLDNHDIDRYSEVVG
                     HDVKKYKMALALLIASRGYPQIYAGNEIMLGGNRGSYEGHRFDFPGGFPGDKRNAFTP
                     EGRTEIENDVFNYLRTLLHYRKNTTALQTGKKKQFIPYDGIYVYFRYDDNATVMVIAN
                     NNDTSRKVKTDRFVEVLAPFTKGKDVTSGKNFDITNSVEIPGKTVLLLELK"
     gene            complement(3228..>3681)
                     /locus_tag="PLD76_04240"
     CDS             complement(3228..>3681)
                     /locus_tag="PLD76_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU27247.1"
                     /translation="SKNRKNGYLDALRKYGIKVDESLVHVCDTREDAIELTQQLLQEP
                     NRPDAFFAINDATASGVLSAVKKAGLRVPEDVSICGFGDGIIASTSDPLLTTVQQNGY
                     QMGVEACNMLLKRIENKLSPTQFNHRLIRTSLVVRESTKPLIESPIHY"
BASE COUNT         1091 a          749 c          646 g         1195 t
ORIGIN      
        1 cccattttgt gcgatggcaa tgaccctgtt aagatagcgt atggcataga taaaatactg
       61 gaccaagcca aacctactcc taacataggc agaagtagta ttgtgggatt ttcaacaaaa
      121 taaatagaaa ttaatcctac agcccctgct atcaaagcta taacatgcgt aaagcgtctg
      181 tttgtatagt tggctagtac tggcaataaa aaggcaacta tagccgcaaa tccattgtat
      241 gctgcaaaca taatgcctac ccaatctgcc ccttcattgt atatagccga atgcgtatcg
      301 ctagtgccat aaatatgtga tgttactgcc gctgtagtat aaatccacat agcaaatagg
      361 gcaaaccatg aaaaaaactg aacaatcgcc aactgtttca tcgttttagg catcaacaat
      421 aggtcgttaa taacttcaac caatccattt ttttgcttgt tggcttgtac taagcctccc
      481 acgaaaaaca gaataagacc gaaaaatgta ataccccctg tcagaatata caactccgct
      541 tccaaactaa aaaaataaac taacactgat aatagtattc ccgtaatagt ccatatagga
      601 gctattttta aaaaacgaga cgatggtaaa attgcttcgt ggtgattact cgtactttcg
      661 ttctcttttt gttcattttt ctcaaatgtt gcgagttctt ctggactata ttcttttgtg
      721 ctaataacag tccatattac tgtaacgaga aaagcgatag ctcccaaata aaacgagtat
      781 tttaccgaag gaggaatgag cccttcgggt gctgtgttgg caatcccaaa ccaattggtt
      841 agcatataag gtaatgcact agctattact gctcctgttc cgataaaaaa actttgcatg
      901 gcaaaaccct ttgtgcgttg ttctgcaggt aacatatctc ccacaaaagc tcgaaacggc
      961 tccatcgaaa tattgataga agcatccatt atccaaagca taccagcagc tacccataaa
     1021 tacggactat tgggcataaa aattaatgct aatgaggcta aaatggcacc tgctaaaaag
     1081 aatggacgtc tacgtcctag cttgttccaa gttttatcac tcatgtgtcc aatgatgggt
     1141 tgaataatta aacctgtaac tggagcagct atccataata ttgggatatt cccaatatcg
     1201 gcaccaagtg tttcaaatat tctacttaca ttggcatttt gtaaagcaaa tccgaactgt
     1261 atgcccaaaa agccaaaact catgttccaa atttgccaaa aacgtaaacg aggtttttcc
     1321 attttgaatg ctgtgttttt taatttgcta ttttaattct aataataaca ctgttttacc
     1381 tggtatctct acgctatttg ttatatcaaa gtttttacct gaggtaacat ctttaccctt
     1441 tgtgaaaggg gctaatactt ctacaaaacg gtccgttttt acttttcggc tagtgtcatt
     1501 attattagct attaccatta ctgttgcatt gtcatcatac ctaaaatata cataaatgcc
     1561 atcgtatggg ataaattgtt ttttctttcc tgtttgcaat gctgtggtgt tttttcggta
     1621 atgcaacaag gttcgtaaat aattaaacac atcgttttct atttctgtac gcccttcggg
     1681 tgtaaaagca ttgcgtttat cgcctggaaa accaccagga aaatcaaaac gatgaccttc
     1741 gtatgagcct ctgttacctc ctagcataat ttcgttacct gcatatattt gtgggtatcc
     1801 tcgcgacgcg attaacagtg ctaatgccat tttgtatttt tttacatcgt gacccactac
     1861 ttccgaataa cgatcaatat cgtggttatc aaggaaattc ataatttgat tggggtcagc
     1921 atacaaaaag tcttgtgcgt aatgattgta aaaacgaatc atgcctctgt cccaactttc
     1981 acgttcgtta aatgaaaatt caaatacatc tttcagtacg aagtccatta cactttgcat
     2041 gtgcgagtca aaaccatatt tattcattga ccctgattga tagaacgcaa tttcttgagg
     2101 cgtttttacc caacattcac ctactatgtt gatagtcgga tattcgtcgc gaatagcttt
     2161 aatccatttt gctcctatac ggatgtctgt atagggatac gtgtcaacac gtatgccatc
     2221 cacatcggca tattctatcc accaaatata ggcttgtttt agataggtaa acagatgagg
     2281 atttgcaaga ttataatctg gcatattttc tgaaaaccat cctttgttta attgttttac
     2341 atcatattca gacgaatacg ggtctgtcca tgcagtcata cgataattag aagaagtgta
     2401 tgtgtcccat ttatttagcc aatcagaggc agggaggtct ttcatccacc aatgtgcact
     2461 tccacaatgg ttaggaacca catctataat gagcttcaat ccattttctt tacactttgt
     2521 cgaaagttct ttgtagtttt cctttgtgcc taaacgagga tccactttgt agtagtctcc
     2581 tgtggcatag tgatggtaag aatagttttt atcgttattg tcgaaaaatg gagttatcca
     2641 tagggctgtt actcctaaat ctgccaaata gggcaatttt tttgtaatcc cctcaatatc
     2701 accaccatgt cggtgtccaa gatcatccct atttacccct tgtatgtagc ctttaacgtt
     2761 atcattttgt aggttcccat tacaaaaacg atcgggcata agtagataaa ttgcatctgc
     2821 agggctaaaa cttggtcgtt gagccgagtt ttctctacgt gcttgcaagg tataggtaaa
     2881 ttttcctact tgtttttttc catttttgaa aacaatatca aatgtaccgg gtttagtttt
     2941 tttgtcgata tgtaagtaca aaaacaaata gtttgggtta tctgttgtct ctctcttaac
     3001 caaagaaaca ccttcgtaca gaaattcgac cttacttgat gatatatttt tgccatatat
     3061 catcaattgt aattgtgtgt cgtgcatacc aatccaccag ctggcaggct ctaaacgttc
     3121 aaatgttact ttctccgatt tggcatatcc actcaggcag agtgttacga ataagaaaac
     3181 gataaggaat gtttttttca tataattata ggttttttta tttgtattta gtaatgtatt
     3241 gggctttcaa ttaagggttt tgtcgattct cttactacta aactggtgcg aattaagcga
     3301 tgattaaatt gggtagggct caatttgttt tcaattcgct ttaacaacat attgcaggct
     3361 tcaacgccca tttggtagcc attttgctgc accgtagtaa gcaatgggtc ggaagtgcta
     3421 gcaataatac catcgccaaa gccacatatc gatacatcct caggcacacg caatcctgct
     3481 tttttaacag cagacaatac tcccgaagct gtggcgtcgt taatagcaaa aaaagcatct
     3541 ggtctgttgg gctcttgcaa aagttgttgc gttagctcaa tagcatcttc acgtgtatca
     3601 catacatgca ccaaggattc gtccacctta atgccatact ttcttaacgc atctaaatag
     3661 ccattctttc tgttcttaga a
//
LOCUS       DAOSMY010000147         3654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25407_length_3654_cov_3.564601, whole genome shotgun sequence.
ACCESSION   DAOSMY010000147 DAOSMY010000000
VERSION     DAOSMY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25407_length_3654_cov_3.564601"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..647
                     /gene="dut"
                     /locus_tag="PLD76_04245"
     CDS             213..647
                     /gene="dut"
                     /locus_tag="PLD76_04245"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOU27248.1"
                     /translation="MQINIVNKSKHDLPEYATPLSAGMDIRANLSEPITLLPLQRKLI
                     PTGLYIELPKGYEAQIRPRSGLALKKGITVLNTPGTIDADYRGEIGVILVNISDEPFV
                     VNDGERICQMVVAEHATVRWQQVEQLDETQRGTGGFGHTGKK"
     gene            657..2408
                     /locus_tag="PLD76_04250"
     CDS             657..2408
                     /locus_tag="PLD76_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27249.1"
                     /translation="MRFFSLTLIFITLVVPVAIATNNQEKLSTQVVDRSAQQPDFDYY
                     FAEALRQKHLGNTAESIDLFLKSYDINPTSAIVSFELGNLFLQQKADDMGFGYIKNAA
                     ELAPQNYFYQEKLALLYRSNGDFSKAIEVLERIQRLFPDKDEVLYELSQLYTQNGQHK
                     KSIQKLDLLEKRIGLNKAITYDKAVQYVLLKNIKQAHKEIDAMLKKTPNDHTLWILKG
                     DIEYERKKYGNAKKYFQKATDIQPESGYVYISWARYYEAIKDFSTSEEYLYKLFGAKD
                     ISFQDKLHYLEILTSYYSKRMDGIEKMHNLYTSLLQSHPMEYVAHTAYAEFLLQQKLV
                     PEAIEHFYTAALLHPSCLECWVYLLQYYTEKNENEKAYQIAKEAIEAVPNHPVLLFYS
                     GMLALMNDQKEDALVQLLEMIKFTDYNEPNYAEMNAAAHAMLVDLYLIYKKDYPKALE
                     QCDLALKLNPSNHVVMNNCAYTLALQNEQLEKAEKLSAQSLEAEPMNVSYLDTYAYIL
                     FKQKRYETALFFIEKAMEYTKDGEKNAVVYERYGDILYFLGQQTKALEMWQKALELTQ
                     DATDTIKQKVNTKKYVE"
     gene            2398..3198
                     /locus_tag="PLD76_04255"
     CDS             2398..3198
                     /locus_tag="PLD76_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOU27250.1"
                     /translation="MSNKSGIRLLLACMLLILVACKTTKIDTAQSVVPIQVPLKKIVA
                     NQQQIQTINASKVSFSIPMQNQQTPLRGSIKARIDSCMQISIQALFGIEVARIHLTQD
                     SLILLDRINQQYVAESFSSFGSTFPVSFRMLQAIILNRLVDVTTKDDFSDFLYPKDEN
                     LHIVSYENSNFAIQYLVNDALHLLRTTVRDAEKRHYMMAEYQQFDTYNTIVYPKKNAI
                     VMLFSQQQYAIDYSIEKVEFNQQIDLSIVIPPRYKQILLADIAKEYFN"
     gene            3204..>3654
                     /locus_tag="PLD76_04260"
     CDS             3204..>3654
                     /locus_tag="PLD76_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27251.1"
                     /translation="MKKILSILFIFASVVLPALSQTVAELEERRKKALKELEITSSLI
                     SKTAEQKKTSLSQINILRVEINARQSLINVMNQELRNIDRNIGQLRKEAQEKQAEMDK
                     LKGEYAKLMYHSYYKKTKYESLMFILSAKSFAESYRRYRYVQQYAEFA"
BASE COUNT         1284 a          622 c          682 g         1066 t
ORIGIN      
        1 tgtgagggta tgattcctat tcgcgattta gacgatgatt attacacata tgacgaaaaa
       61 aattattgcc ttataggcac acgcttacac aagcgttacc agctaggcga tgaagtggct
      121 gtgctggtag ccaaagccaa tttagaaaag aaacagctag attttgtatt agcataagta
      181 ttacgtaact attttgaata tataaagaaa tgatacagat taacattgta aacaagtcaa
      241 aacacgattt acccgagtat gctacgccct tatctgctgg aatggatata cgtgctaacc
      301 tttctgagcc cattactttg ctacctttgc aacgtaaatt aattcctacg ggtttgtata
      361 tagagctacc caaggggtac gaagctcaaa ttcgaccacg aagcggactt gctctcaaaa
      421 aaggcattac agtgctcaat actcctggca cgattgatgc cgattatcgc ggtgaaatcg
      481 gtgttatttt ggttaatatt tcggacgaac cgtttgttgt aaatgatggc gagcgtatat
      541 gccaaatggt agtggcagag cacgcaactg ttcgttggca acaagtagaa cagcttgatg
      601 aaacacaacg tggtactggc ggttttggtc atactggtaa aaaatagatg atacgaatga
      661 gatttttttc actaactctt atattcatta cgcttgttgt gcctgtagca atagctacca
      721 acaatcagga aaaactatcc acacaagtgg ttgataggtc agctcaacag cccgatttcg
      781 attattattt tgcagaagct ttgcgacaaa agcatttggg aaatactgcc gaaagtattg
      841 atttgtttct caaaagttat gatatcaatc ctactagtgc gattgtttct tttgaattag
      901 gcaatttgtt tttgcaacaa aaagccgatg atatggggtt tgggtatatc aaaaacgcag
      961 cagagttagc tcctcaaaac tatttctatc aagaaaaact tgcgttgctc taccgtagca
     1021 atggcgattt ttctaaagcc atagaagtgt tagagcgtat tcagcggtta ttcccagata
     1081 aagatgaggt tttatatgag ttatctcaat tgtatacaca aaatggacaa cacaaaaaat
     1141 caatacaaaa acttgatttg ttggaaaaac gtatagggtt aaacaaagca ataacgtacg
     1201 ataaagcggt acaatacgta ttgttgaaaa atataaagca agcacacaaa gaaattgatg
     1261 caatgcttaa aaaaacgccc aatgatcata ctttatggat actgaaaggg gatatagaat
     1321 acgaaagaaa aaaatatggt aatgcaaaaa aatattttca aaaggctact gatattcaac
     1381 cagaaagtgg ctatgtatac atctcttggg ctcgatatta cgaagcgata aaggatttta
     1441 gcacttcaga agagtactta tataaacttt ttggagcgaa agatatttcg tttcaagata
     1501 aattgcatta tttagaaata ctaaccagct attacagcaa aagaatggat ggaatagaaa
     1561 aaatgcataa tttatatact tctttattgc agtcgcaccc catggagtat gtagctcata
     1621 ctgcatatgc tgaattttta cttcaacaaa aattagtccc tgaggcgata gagcatttct
     1681 atacagcagc tttactccat ccttcgtgtc tcgaatgttg ggtatattta ctacaatatt
     1741 atacagaaaa aaatgaaaat gaaaaagcct atcaaatagc gaaagaggcg atagaagccg
     1801 tacccaacca tcctgtgcta ttgttttatt caggcatgct tgctttgatg aatgaccaga
     1861 aagaggatgc tctggttcag ttacttgaaa tgattaaatt taccgattat aacgagccta
     1921 attatgctga aatgaatgca gcggcacatg cgatgttagt cgatttgtat ctgatatata
     1981 aaaaagatta cccaaaagct ttagagcagt gcgaccttgc tctaaaatta aacccatcaa
     2041 atcatgtggt aatgaataat tgtgcctaca cactcgcatt gcaaaatgag caacttgaaa
     2101 aagccgagaa attaagtgct caatctttag aggcagagcc tatgaatgtg tcctatttag
     2161 atacgtatgc atacatttta tttaagcaga aacgctacga aacggctcta ttttttatcg
     2221 aaaaagccat ggaatatacc aaagatggag agaagaatgc agtggtttac gaacgctatg
     2281 gcgatatctt atatttttta ggacaacaaa ccaaagcact cgaaatgtgg caaaaagccc
     2341 ttgaattaac ccaagatgct acggatacaa taaaacaaaa agttaacacc aaaaagtatg
     2401 tcgaataaat caggaatcag attgttgtta gcatgtatgt tgctcatttt ggtggcgtgt
     2461 aaaacaacaa aaatagacac tgctcagtct gtggtgccta tacaagtacc gttgaagaaa
     2521 attgtggcaa accaacagca aatacagacg ataaatgctt cgaaagtaag cttttctatt
     2581 ccaatgcaaa accaacaaac cccactacga ggctctatca aagcaaggat tgatagttgt
     2641 atgcaaattt ccatacaagc tttgtttgga atagaagttg ctcgcataca ccttacgcaa
     2701 gatagtctta ttttacttga cagaataaat cagcaatatg ttgctgaatc tttttcgtcg
     2761 tttgggtcaa cgtttccagt ttcttttcgt atgttgcaag caattatcct taatagatta
     2821 gtagatgtaa cgacaaaaga tgatttttca gattttttat acccaaaaga cgaaaatcta
     2881 cacatagtat cgtacgaaaa tagtaatttt gcgattcaat accttgtaaa tgatgctctt
     2941 catttgctac gaacaacagt aagggatgcg gaaaaaaggc actatatgat ggcagaatac
     3001 cagcagtttg acacatataa tacaatagta tatccaaaga aaaacgctat tgtgatgctc
     3061 ttttcccagc aacaatatgc tatagattat agtatcgaaa aagttgagtt taatcaacaa
     3121 atagatttat caattgttat tcctcctaga tacaaacaaa ttttgctggc tgatattgcc
     3181 aaagaatatt ttaactaata attatgaaaa aaatactttc tattctattt atttttgcca
     3241 gtgtggtatt acctgctctt tctcaaactg tagcagagct tgaagagcgt cgtaaaaaag
     3301 cccttaaaga attggaaatt actagttcgt tgattagtaa aactgctgaa cagaaaaaga
     3361 cctctttgtc tcaaattaat attttgcgtg tagaaattaa tgcccgtcag agtttaatta
     3421 atgtgatgaa ccaagaactt cgtaatatag atagaaatat tggtcagtta cgcaaagagg
     3481 cacaagaaaa acaagcagaa atggacaagt taaaaggtga gtatgctaaa ttaatgtacc
     3541 attcgtatta caaaaaaacc aaatatgaat ccctgatgtt tattttgtca gctaaaagtt
     3601 ttgccgaatc gtatcgcaga taccgctatg tacaacagta tgctgaattt gctc
//
LOCUS       DAOSMY010000148         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25482_length_3646_cov_3.287385, whole genome shotgun sequence.
ACCESSION   DAOSMY010000148 DAOSMY010000000
VERSION     DAOSMY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25482_length_3646_cov_3.287385"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2313)
                     /locus_tag="PLD76_04265"
     CDS             complement(1..2313)
                     /locus_tag="PLD76_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU27252.1"
                     /translation="MKNILIILLCFLSASTLAQVNETFSDGNFSHAPIWEGNASNFVI
                     NSNAQLQLNAPAEANKSYLVTNSNVTTNAEWKMYGKMMFTPSSANYARWYLIADKQDI
                     TGSLNGYYLLIGNTSKNICLYKQTGTTSTKILDTPINRLNGTTNEINIRITRSSEGLW
                     QLFTQLPTENSPILEGTTTDNTHKAALYTGIVCYYTATRNTGFIFDDIFISGETYEPP
                     ILPSYKASFGDIVFNEIMANPNPPFGLPNEEYIELYNNTSEAINLENYVLKGFSKTCV
                     FPTYVLAPHSYVVVCSTKAFPALEMYGNCLALDKFPTLTNAGGYLELFNAENTILSWV
                     DYSENWHTDSFKKNGGWSLECLDAHNLIGNNTNWNSSINYQGGTPNGANSISGINIDT
                     SELSVINTSIESNSSFVLYFNKPMSVNLLTQTDIITIYPMREISSIEFLSIKQDAIRI
                     TLTNPLLLHEQYTLNITDVEDINKNKHSIVSRLAIPELPSNQDVVINEVLFNPKSEGT
                     DYVEIVNRSQKVIDLRDIMLTNRKQDGQLAVFPVLSEKGYLLFPGDYCLLSTSKEGVC
                     NYYECPDDIHFVTVPSFPSMPDDKGTIVLTTLSNILIDEFSYSEKMHHTLLSNREGIA
                     LERLHYDITTQDASNWHSASFTCGYGTPGYQNSQFTALIQPTASFQITPKSFSPNNDG
                     NDDTATIYYSFDEAGYFVTIRIYNSNGNCVRNILQNNLSSTEEKTSWDGTDDNGKILP
                     IGIYIVTIEAFTSTGKRFKSKEVIVLAAKI"
     gene            2564..3571
                     /locus_tag="PLD76_04270"
     CDS             2564..3571
                     /locus_tag="PLD76_04270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU27253.1"
                     /translation="MKVAIVGASGAVGQEFLRVLAERNFPMDELVLFGSSRSAGSEYE
                     FKGKKLVVQELKHGDDFKGVDIAFTSAGAGISKEFAADITKFGAVMIDNSSAFRMDTD
                     VPLVVPEVNAADAKNRPRNIIANPNCTTIQMVVALQAIENLSHVKRVHVSTYQAASGA
                     GAAAMDELELQYKQVLHGETPTVSKFAYQLAYNLIPQIDVFTDNGYTKEEMKMYHETR
                     KIMHSDIEVSAMCVRVPSLRAHSEAIWAETERPISVEEAQAAFAKATGVILQDNPAQK
                     EYPMPLFVSGKDPVYVGRVKKDLANPNGIFFWTVSDQIKKGAALNAVQIAEYLIAEGN
                     I"
BASE COUNT         1149 a          584 c          730 g         1183 t
ORIGIN      
        1 ttagattttt gctgccaaca caatgacttc tttcgattta aagcgttttc ctgtagatgt
       61 aaacgcttca atagtaacaa tatatattcc aatgggtaaa atctttccgt tatcatctgt
      121 tccatcccaa gatgtttttt cttctgtgga tgataagtta ttttgcagta tatttcttac
      181 acaatttccg ttcgagttat atatgcgtat agttacgaaa tagccagctt catcaaagga
      241 ataatagatg gttgctgtgt catcgtttcc atcgttattg ggagaaaaac ttttgggggt
      301 aatttgaaac gaagcggtgg gctgtatgag tgctgtaaat tgtgaattct gataacctgg
      361 tgtgccatac ccacaggtaa aagaagctga atgccagttg cttgcgtctt gtgttgtaat
      421 atcataatgg agtctttcta gggcaatacc ttctctgttt gagagtaagg tgtggtgcat
      481 tttttcggaa tacgaaaatt catcaatcag tatgtttgat aaagtagtaa gcacgatagt
      541 tcctttatca tcaggcatgc ttggaaatga gggtactgtt acaaaatgaa tatcatcagg
      601 gcattcataa taattacaaa cgccttcttt gctagtgctt agtaggcaat aatcaccagg
      661 aaacaataag tagccttttt cgctcagtac tggaaaaacg gctaattgtc catcttgttt
      721 cctatttgta agcatgatgt ctcgcaaatc aattactttt tgcgaacgat taacaatttc
      781 gacataatcc gtcccttcag attttgggtt aaataaaacc tcgttgataa caacatcttg
      841 atttgaagga agttcaggta tggctaagcg actaacgata gaatgtttgt ttttattaat
      901 atcttcaacg tcagtaatgt ttaatgtgta ttgctcatgt aatagaagtg gatttgtaag
      961 tgtgatgcgt attgcgtctt gttttatgga taaaaattcg atagaactta tctctcgcat
     1021 tgggtatata gtaatgatat ccgtttgtgt gagaagattc acagacattg gtttattgaa
     1081 atatagtaca aaagaactgt ttgattcaat gcttgtgttt ataacagaca actcggatgt
     1141 atctatgttt atacctgaaa tactatttgc tccatttgga gttcctcctt gataattgat
     1201 gcttgagttc caattagtat tgttcccaat taaattatgt gcatcgagac attctaaact
     1261 ccaacctcca ttttttttga aagaatcggt atgccaattt tccgagtaat cgacccatga
     1321 aagtatggta ttttctgcat tgaataattc aaggtatcca cctgcattgg tgagagttgg
     1381 aaatttgtcg agagccaaac aattaccata catttctaaa gcgggaaaag ccttggtgct
     1441 acatactaca acgtagctat gaggagccaa tacatacgta gggaaaacgc atgtttttga
     1501 aaatcctttt aatacatagt tttctaaatt gatagcttcc gacgtattgt tgtagagttc
     1561 aatatactct tcgttgggta atccaaaagg aggatttgga tttgccataa tttcattaaa
     1621 aacaatgtct ccaaacgatg ctttatagct gggcaaaatt ggaggttcat atgtttctcc
     1681 agagataaag atatcatcaa aaatgaatcc cgtatttcgt gttgcggtgt aatagcaaac
     1741 aataccagta tatagagcag ctttgtgcgt attgtccgtt gtagtgcctt ctaaaatagg
     1801 cgaattttct gtaggtaatt gtgtaaataa ttgccataac ccttcgcttg aacgggtaat
     1861 gcgaatgtta atttcgttag tagtgccatt taatcggttg atgggtgtat cgagtatttt
     1921 ggtggaggta gtacctgttt gcttatacaa acaaatattt tttgacgtat taccaataag
     1981 caaataatat ccgttcaatg agccagtaat gtcttgtttg tcagcaatta agtaccaacg
     2041 agcataatta gcagaacttg gtgtaaacat cattttgcca tacattttcc attcggcatt
     2101 ggttgtaaca ttcgaatttg taactaaata cgatttattt gcttctgcag gagcatttag
     2161 ttgaagttgt gcattgctat tgattacaaa attggatgcg ttaccttccc aaatgggagc
     2221 atgcgaaaaa ttaccatcag aaaatgtttc attcacttga gccaaagtac ttgccgatag
     2281 gaaacaaaga agaataatga gaatgttttt catggctata aaaatagagc gacaaagtaa
     2341 ctattatttt tttgtttgtt aaaatgttag ccatcaaaaa agccttgttg gcatcgatga
     2401 gtgttgcttt ttgtgctaaa aagcataaaa aatactattt tttagtttat ttgtttaata
     2461 attagaaagt aaagcgtaat tttgtagtca gtttgcgtcg aaaaagaaat tttgactaga
     2521 ggatgcaaat acttaataag taaacgttat aaaacagtaa aaaatgaaag tagcaattgt
     2581 tggtgctagc ggtgcagtag gtcaagagtt tttgcgtgta ctcgctgagc gtaatttccc
     2641 aatggatgaa ttagtattgt ttggttcatc acgtagtgca ggatcagaat atgaatttaa
     2701 aggcaaaaag ttggttgtgc aagaattaaa gcatggcgac gattttaagg gtgtagatat
     2761 tgcctttaca tctgctggtg caggtatttc aaaagaattt gcagccgata ttacaaagtt
     2821 tggtgctgta atgatagata attctagtgc atttcgtatg gatacagacg tgcctttggt
     2881 agtgccagaa gtgaatgctg cagatgcaaa aaatagacca cgcaatatca ttgcaaatcc
     2941 aaattgtact actatacaaa tggtagttgc attgcaagca atagaaaatt tatcgcacgt
     3001 taaacgtgtg catgtttcta cttaccaagc ggctagtgga gcaggagctg cagctatgga
     3061 tgaattagaa ttacaataca aacaggtgtt acatggagaa accccaactg tatccaaatt
     3121 tgcttaccaa ttggcatata accttattcc acaaattgat gtatttaccg ataatggata
     3181 taccaaagag gagatgaaaa tgtatcacga aacacgcaaa attatgcact cagatattga
     3241 agtaagtgcc atgtgtgtgc gtgtcccctc gttaagagca cattcggaag caatttgggc
     3301 agaaaccgaa cgtcctattt cggtagaaga agcacaagct gcttttgcta aagctactgg
     3361 tgtaattttg caagataatc ctgcacaaaa agaatatcca atgccactct ttgtatcagg
     3421 caaagaccct gtatatgtag gacgtgtaaa gaaagactta gccaacccaa atggaatttt
     3481 cttttggaca gtgagcgacc aaattaaaaa aggtgcagct cttaatgccg ttcaaatagc
     3541 agaataccta attgcagaag gtaatattta atcataagat actaaacggg tgccaaaaag
     3601 cattcgttta gtttttaata ctattctaat tatgtcgcaa caacgc
//
LOCUS       DAOSMY010000149         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25486_length_3646_cov_2.860485, whole genome shotgun sequence.
ACCESSION   DAOSMY010000149 DAOSMY010000000
VERSION     DAOSMY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25486_length_3646_cov_2.860485"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..>1770)
                     /locus_tag="PLD76_04275"
     CDS             complement(279..>1770)
                     /locus_tag="PLD76_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU27254.1"
                     /translation="ALIGKNTPHWAITFVATVTYGAVIVPILQDFNPNDVHHIVNHSE
                     TVFLFCSDSIWDNLEEDKMENIQAAFSIDDFRCIHQKDGAGIQKIVGNLTSKFEKKYP
                     QGFTQSHIQYTSRDNSELAMLNYTSGTTGFSKGVMLTANNLAGNITFGFKTQLLKKGD
                     RVLSFLPLAHAYGCAFDFLVPTCSGSHIYFLNKIPSPKVLLKAFEEIKPNVIFMVPLI
                     LEKVYKKSIQPSLNKTGIKLALNIPLLNKKIYEQVNKKLTESFGGEFTEIVVGGAALN
                     KEVEEFLTKIGFRFTIGYGMTECAPLISYASHTEFRPSSAGKILDTMQVKIDSPDPYN
                     IVGEILVKGEHVMQGYYKNELATSEAFTDNGWLKTGDLGTIDEHGYIFIKGRSKSMIL
                     GANGQNIYPEEIEAKLNNLPFIMESLVIEKHGKLIALVYPDYEAMDGSQLTQQDLEII
                     MEENKNNLNNVVGTYENVSKIHLYPNEFEKTPKRSIKRYLYTNISI"
     assembly_gap    1771..1780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1781..1946)
                     /locus_tag="PLD76_04280"
     CDS             complement(<1781..1946)
                     /locus_tag="PLD76_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27255.1"
                     /translation="MIKENFIKLFETSFKENWELPAYTDYTLGTSMTYGEVALKIEKL
                     HLLFAESKIKP"
     gene            complement(2043..2822)
                     /gene="mazG"
                     /locus_tag="PLD76_04285"
     CDS             complement(2043..2822)
                     /gene="mazG"
                     /locus_tag="PLD76_04285"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU27256.1"
                     /translation="MKKKLEAVENVIHILDELRVKCPWDKKQTNETLRVLTIEETYEL
                     SDAILQGNNQQIKKELGDLLLHILFYAKIGEEKSAFTLKDICDALAEKLIYRHPHIFG
                     TEQAQTSQEVEKNWEKLKLKEKEGNKTILSGVPSSLPSIIKAHRIQDKARSAGFDWEK
                     KEDVWKKVQEEISELTVEIDSMNKEKMEKEFGDVFFSLINAARLYKINPDNALEHTNK
                     KFIQRFTYLEEYAKQHEIALSDMPLAKMEEIWQLAKKEENM"
BASE COUNT         1170 a          529 c          528 g         1409 t
ORIGIN      
        1 aacttcttct tctactacag cttcttcagt agctttttct gcacatgaag caaaagcaac
       61 agcaacgata gtagcaacaa ataatactaa ctttttcatt tttttagttt ttaaaatatt
      121 aaacaatgat tttttttgaa cgcacaaaga tatccattta ttttgtaaca aaaataacaa
      181 aaataaaaaa aataaaaaaa ctcttgttaa taagtgttaa taatacgtta gagagcaaat
      241 gtagtataca taacggtgta aataaatgat aaaattcctt aaatactgat atttgtatac
      301 aaatagcgtt taatgcttct tttgggtgtt ttctcaaatt cattaggata taggtgtatt
      361 tttgacacat tttcgtacgt acctactacg ttgtttagat tgttcttatt ttcttccata
      421 ataatttcta aatcttgttg tgtaagttgt gaaccatcca ttgcttcgta gtctgggtaa
      481 actagagcaa taagtttacc atgtttttct attactaaac tttccataat aaatggtaaa
      541 ttatttagtt ttgcttctat ttcttcaggg tatatatttt gtccattagc ccctaaaatc
      601 atgctttttg atcttccctt aataaaaata taaccatgtt catcaatagt tcctaaatcg
      661 cctgttttca accatccatt gtcggtgaat gcttcggatg tagccagttc gtttttataa
      721 tatccctgca tgacatgttc tccttttact agtatttctc ctacaatatt gtaagggtcg
      781 ggtgaatcaa tcttaacttg cattgtatct aatattttac cagctgagga aggtctaaat
      841 tcagtatggc ttgcatatga aataagtgga gcacattctg tcattccata cccaatggta
      901 aaacgaaacc caattttagt taagaattct tctacttctt tgtttaatgc agcaccacca
      961 acaacaattt cagtgaattc gccaccaaat gattcagtaa gtttcttatt tacttgttca
     1021 taaatctttt tatttagcaa tgggatattt agagctagtt taatacctgt tttatttaac
     1081 gaaggctgga tagatttttt atatactttt tctaaaataa ggggaaccat aaaaataacg
     1141 ttaggtttta tttcttcaaa tgcttttaac aaaacttttg gacttggtat tttattcaaa
     1201 aaatatatat gagaaccaga acatgtggga acaagaaaat cgaaagcaca accataggcg
     1261 tgagctaatg gtaaaaaaga taatactcta tctccttttt taagaagctg ggttttgaag
     1321 ccaaaagtaa tattccctgc tagattattg gctgttaaca taactccttt actaaatcct
     1381 gtagtgcctg atgtataatt tagcatggca agttcactgt tatcgcgtga tgtgtattgt
     1441 atatggcttt gggtaaagcc ttgcggatat ttcttctcaa acttgctagt tagattgcct
     1501 actatttttt gaattcctgc accatctttt tgatgtatac atcgaaaatc gtcaatggaa
     1561 aatgctgctt gaatattttc cattttatct tcttctaaat tatcccaaat actatcacta
     1621 caaaataaaa agacagtttc agagtgatta acaatatgat gtacgtcatt gggattaaaa
     1681 tcttgcaaaa taggtacaat tacggcacca taagttactg tagctacaaa tgtgatagcc
     1741 caatgtggtg tatttttgcc aatcaaagca nnnnnnnnnn caggcttaat tttagattct
     1801 gcaaacaaaa ggtgtaattt ttcaattttc agtgcgactt ctccatacgt catgctagta
     1861 cccaaagtat aatctgtgta ggcaggtaac tcccaatttt ccttaaaact agtttcaaag
     1921 agcttgataa aattttcttt aatcatagta ttttgattta tgttgtttta caaaaataaa
     1981 ctattttttg atacatatag tttgtaggct aacttttaag aaattttgtt agtaaaacga
     2041 tgctacatgt tttcttcttt ttttgctagt tgccatattt cttccatttt ggcaagaggc
     2101 atatcactaa gagctatttc atgttgtttt gcatattctt ctaagtaggt aaaacgctga
     2161 ataaattttt tattggtatg ttctaaggca ttatctgggt taattttata caatctagca
     2221 gcattaatta aactgaaaaa tacgtcacca aactcttttt ccattttttc tttgttcatt
     2281 gagtctattt ccacggttaa ttctgaaatt tcttcttgca cttttttcca cacatcctct
     2341 tttttttccc agtcaaaacc agcacttcga gctttatctt ggatgcgatg agcttttata
     2401 atggacggaa gagaggatgg aacgcccgat agtatcgttt tgtttccttc tttttctttt
     2461 aattttaatt tttcccaatt tttttctacc tcttgagagg tttgagcttg ttctgtccca
     2521 aaaatatggg gatgtctgta tataagtttt tcggcaagtg catcacagat atcttttagt
     2581 gtaaaagctg atttttcttc tccaattttt gcgtagaata atatatgcag taataaatcg
     2641 cctaattctt tttttatttg ttggttgttg ccttgaagta tagcatctga caattcgtac
     2701 gtttcttcaa tagtaagaac tcgaagggtt tcgttagttt gttttttatc ccaaggacat
     2761 ttaactctta attcatctaa tatatgaatg acgttttcaa cagcttctaa tttttttttc
     2821 atacttatat tcttatttac gatttctcga tagggcagta caaaactcgt aaataatatt
     2881 tagttaaaca aatttcttct acatcactta tcgactttta gtgcttaaat tcttgcataa
     2941 atgcacgcaa cgttacaaat atgaaattct agggagttta aatacgtgat aattgtatga
     3001 tttttaagta tttatgtatg taatttgtta ttaacaatat aaacaaaata ggaaatggta
     3061 cataatttta aaataatggc atcacgtaag tctattttaa cagattattg attacatatg
     3121 taatataaca agatgttaaa gttaattaca cattaatttt agggaaacat agagtagggt
     3181 aattgtttat tttcacattg aattacattt gtatttttaa tagaatcata ctatagattt
     3241 aagaaatata ataattatta atttattgta tattagatat ttataaattt attataaagt
     3301 atttatttat atttaatggt ttttacctcc tagtagtaga taaaacgcaa aatcacaata
     3361 aaaattgatt attattatat tgttagacag tattttattg taaatatata aagtattagt
     3421 ttttatttta ttgattgatt ttggtggaat atgacactga atgataaata ctatttttta
     3481 atttgatttt acagatgtaa atcaaatggc actgaaaata cagttgtaat atttgcaaat
     3541 gtaataaatt gtaattacat ttgtgataaa atatattaca tgaaagatag gttaaaagaa
     3601 cttttagaga aagtattgtt agaagccgaa ttattagatt taaaag
//
LOCUS       DAOSMY010000150         3623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_25723_length_3623_cov_3.131446, whole genome shotgun sequence.
ACCESSION   DAOSMY010000150 DAOSMY010000000
VERSION     DAOSMY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25723_length_3623_cov_3.131446"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1208)
                     /locus_tag="PLD76_04290"
     CDS             complement(24..1208)
                     /locus_tag="PLD76_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOU27257.1"
                     /translation="MKQYNFDEVIDRTNTSAYKLVLMEKYFGSNDAIPLWVADMDFRT
                     PDFILDAIKNRCDSGVLGYTLPSADYTTSIAKWLKYKHDWEVSEKFVAFAPGVVTGLA
                     LAIRAFTNPHDKILVQTPVYPPFLSLPNEHNRELVINPLQFHSNTFEIDFFDFEKKLA
                     SGVRMFILCHPHNPGGRVWNADELMRMAKLCIQYNVLMVSDEIHADLMLWGKQHIPLA
                     KLLPEIAQHTITFMAPSKTFNIPGLASAFFVAENPQLFAKMQQETASFHLNMGNVFGC
                     MATEVAYNEGKEWLNQAIHYIEQNILFVRNYIEQHIPIIQVVVPDASFLVWLDCRKMG
                     LTSKELKEFMVHKAKVIMNDGATFGQGGEGFQRLNVACPRSVLQQALQQIETAINEMF
                     TK"
     gene            complement(1223..2548)
                     /locus_tag="PLD76_04295"
     CDS             complement(1223..2548)
                     /locus_tag="PLD76_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU27258.1"
                     /translation="MAVKKNFVLDTNVILHDHKCIYKFQEHDIYLPIVVLEELDKFKK
                     GNEEINFNARQFARELDLVTDNDLFTKGASLGEGLGRLFIVLGVPFSDTMQVSFMEKT
                     PDHRILAITQALQEKNKKQKTILVSKDINLRMKARSLGILAEDYFNDKITSKDIFDKE
                     HETFDNIDGDVIDRIYSTAAGVPLAQFEFQSDVQPNDYFVLKSERNSVLARYNPFFET
                     VVKVRKEKAFGIEPRNAEQSFAINALLDDEIKLVCITGKAGTGKTLLALAAALEKNNE
                     YKQILLARPIVALANKDIGFLPGNQKEKIAPYMQPLFDNLNVIKHQFAVESREQLLIK
                     EMLVSEKLVIEALAFIRGRSLSDAYIIVDEAQNLTPHEIKTIITRAGENTKVVFTGDV
                     QQIDSPYLDMFSNGLVYAIDKMKGQHIFAHVNLVKGERSYLSELASNLL"
     gene            2684..3355
                     /locus_tag="PLD76_04300"
                     /pseudo
     CDS             2684..3355
                     /locus_tag="PLD76_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
BASE COUNT         1175 a          719 c          559 g         1170 t
ORIGIN      
        1 aattattcgt ttcatatttt ttattattta gtaaacattt cgttaatcgc agtttctatc
       61 tgctgaagag cttgttgtaa tacactacgt ggacatgcta cattcagtcg ctgaaaaccc
      121 tctcctcctt gaccaaaagt agccccatca ttcattatta cctttgcttt atgaaccata
      181 aattctttta attctttgga agttaaaccc atttttctgc aatcaagcca tactaaaaag
      241 gatgcatcgg gcacaactac ttgtatgata ggaatatgct gttcaatata attgcgtaca
      301 aatagtatgt tttgttcaat ataatgtata gcttggttta accattcttt cccttcattg
      361 taagccactt ctgttgccat acagccaaat acatttccca tattgagatg aaaactagct
      421 gtttcttgct gcattttggc aaaaagttgc ggattctcag ctacaaaaaa agcactagct
      481 aaaccaggta tgttaaatgt tttactaggt gccatgaaag taatggtatg ttgagcaatt
      541 tcgggcaata atttggctaa aggaatatgt tgtttacccc acagcatcaa atctgcatgt
      601 atttcatccg acaccattaa cacattgtac tgaatacaca atttagccat acgcatcaac
      661 tcgtcagcat tccacactct tcctccagga ttgtgtggat gacataaaat aaacatgcga
      721 acaccagagg ctaatttctt ttcaaaatca aaaaaatcta tttcaaaggt attcgaatga
      781 aattgtaagg gattgattac taattctcta ttatgctcat taggtaaact caaaaaaggt
      841 ggatacacag gtgtttgtac caatatttta tcatgagggt tggtaaaagc acgaatagct
      901 aacgccaaac cagtaactac gccaggagca aaagcaacaa atttttcaga gacctcccaa
      961 tcatgtttat atttcaacca ttttgctatt gacgttgtat aatctgccga aggcaaggtg
     1021 tagccaagca ccccgctatc gcagcgattt tttatcgcat caagaataaa atccggtgta
     1081 cgaaaatcca tatctgccac ccataatggt atggcatcat tagagccaaa atacttttcc
     1141 atcaacacca acttatacgc agaggtgttt gttctatcta ttacttcgtc aaaattgtat
     1201 tgtttcattc ttccacatat agttatagca aattacttgc taattcagac aaataactac
     1261 gttctccttt cacaagatta acatgtgcaa aaatatgttg ccctttcatt ttatcaatgg
     1321 catataccaa tccattagaa aacatatcca aatacggaga atctatctgt tgcacatcgc
     1381 cagtaaatac cacttttgta ttttcacctg cacgggtaat gattgtttta atttcgtgtg
     1441 gggttaagtt ttgtgcctca tctacaataa tatatgcatc agacagactt ctacctctta
     1501 taaatgcaag agcttcaatc actaattttt ctgacaccaa catttctttt atcagcaatt
     1561 gctccctact ttctacggca aattgatgtt taattacgtt gaggttatca aacaacggtt
     1621 gcatatacgg tgcaattttt tctttctggt tgcctggcaa aaaaccaata tctttgttag
     1681 ccaaggctac aattggacgt gctaacagga tttgtttgta ctcattgttt ttctccaagg
     1741 ctgcggcaag tgctaacaat gtttttccag tacctgcttt tcctgtaata caaacgagtt
     1801 tgatttcatc gtctaacaag gcattaatag caaaactttg ttcagcattg cgtggttcaa
     1861 tcccaaatgc tttttctttc ctcactttta caacggtttc aaaaaatgga ttgtaacgag
     1921 caagtaccga attacgttca cttttcaaca caaaataatc gttgggttgc acatcactct
     1981 gaaattcaaa ttgtgctaaa ggtacaccag cagcggtact gtaaattcta tcaatcacat
     2041 cgccatcaat attatcaaac gtttcgtgct ctttgtcaaa aatatctttg ctcgttattt
     2101 tatcgttaaa ataatcttct gccaaaatgc ccaacgaacg agccttcata cgcaaattga
     2161 tatctttact tactagaatg gttttttgct ttttattttt ttcttgtaag gcttgcgtaa
     2221 tggctaaaat acgatgatct ggcgttttct ccataaaaga cacctgcatt gtatcggaaa
     2281 aaggtacacc caacacaata aacaaacgtc ctaaaccctc tcccaaagaa gctcctttgg
     2341 taaataaatc attatcagta accaaatcaa gttcgcgggc aaactgccga gcattgaaat
     2401 taatttcctc gtttcctttt ttaaacttat ctaactcttc taacacaaca attggtaaat
     2461 aaatgtcatg ttcttgaaat ttatagatac atttgtgatc gtgtaaaatt acattggtat
     2521 ctaacacgaa attctttttt actgccatag tattcgtctt ttggggttga ttttgttaaa
     2581 aacacgtgta aatatagtat aaaaaaaaga taaaacgtaa ttttacatcg tctttttata
     2641 aacattaata aagtcaatat actgttacaa ttatcaacta actatgacat acgaacaaac
     2701 cctacaatac ctgtacgaaa aaacacctct atatcaacac attggcaata aagcctacaa
     2761 accgggatta gataatatta aacaactcga cacccatttt gaacatcctc atactgcttt
     2821 caaaaccata catgtgggcg gcactaatgg caaaggctcc gtttcgcatt cattagcagc
     2881 cgtgctacaa tcgaatggct ataaggtagg gttatatact tctccgcatt tacaagattt
     2941 tgccgaacga atacgtgtaa atggagaaaa gattagtaaa aactatatta ttgattttgt
     3001 aagtaactcg cagaaatata tcgaacaaat gagcctttca ttttttgaac tcaccacaat
     3061 gatggcattg tgctatttta aggaacaaaa ggtggattat gctatcatag aagtaggtct
     3121 tggaggtaga ttagatagca caaatatcat cactcctata gcaagtgtca ttacaaatat
     3181 tagtttcgat catacctatt tgcttggcaa cacattgaaa gagattgcta cagaaaaagc
     3241 aggcattatc aaacaaaaca cacccatcat aattggagag aaacaagaag aaacaaatgc
     3301 agtatttaag aataaggcaa aagaaatgaa tgcccctata ttttttgccg aagagatata
     3361 ccaactttct caattaaaag aagaagctga acatacggta tatagtattg ataattttgg
     3421 taagctccct ctttgtctaa agcaatacca gcctttaacc cagcctcaag tagtaatact
     3481 ttttttccag ctctggtaag tgttttcgcc attattgccc ctgctattcc tgagccaact
     3541 attacaacat catattcatt attttccata attggtatta gtgttttgta ggtattttta
     3601 aaggtggaat actccaagat cca
//
LOCUS       DAOSMY010000151         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26037_length_3589_cov_3.597057, whole genome shotgun sequence.
ACCESSION   DAOSMY010000151 DAOSMY010000000
VERSION     DAOSMY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26037_length_3589_cov_3.597057"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="nqrC"
                     /locus_tag="PLD76_04305"
     CDS             <1..361
                     /gene="nqrC"
                     /locus_tag="PLD76_04305"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOU27259.1"
                     /translation="DGETKYIFPLYGAGLWGPIWGYIALNHDKNTVYGVYFSHAGETP
                     GLGAEITSHVFQSQFPNKKIKNTTNDLVSIAIVKPGKTAEGQDYVDGISGGTITSHGV
                     ELMLKNTLVNYTSFLNK"
     gene            421..1053
                     /locus_tag="PLD76_04310"
     CDS             421..1053
                     /locus_tag="PLD76_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOU27260.1"
                     /translation="MKSLFSAKNKEVIVGPLSLNNPITVQVLGICSSLAVTAKLEPAI
                     VMGMSVTIIVAISNVIISFMRNTIPVRIRIIVQLVVVATLVTVVSQFLKAFAYDVSVQ
                     LSVYVGLIITNCILMGRLEAFAMSNRPWESFLDGLGNGFGYALILVIVAFFRELLGSG
                     SLLGFHVIPASVYESGYINNGLMVMPPMALIILAIIIWAHRTKVKQLQDN"
     gene            1079..1678
                     /gene="nqrE"
                     /locus_tag="PLD76_04315"
     CDS             1079..1678
                     /gene="nqrE"
                     /locus_tag="PLD76_04315"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOU27261.1"
                     /translation="MENLSSLFVKSIFVDNMIFAYFLGMCSFLAVSKNVKTAFGLGVA
                     VTFVQVMTLPVNYLLENYILKPDVLIEGVDLSFLSFIVFIAVIAGITQLVEMMVEKFT
                     PALYASLGIFLPLIAVNCAIMGGSLFMQQRNFTSISEASVFAVGSGLGFLVAIVGLAA
                     IREKMAYSHVPAPLKGLGITFIVVGLMAMAFMCFSGLKV"
     gene            1719..2981
                     /gene="nqrF"
                     /locus_tag="PLD76_04320"
     CDS             1719..2981
                     /gene="nqrF"
                     /locus_tag="PLD76_04320"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOU27262.1"
                     /translation="MTFLSVSPIMIGTSIVVFLAVILLLVVILLFAKKYLSPSGPVKI
                     LINGEKEIEVESGSTLLSTLANEKIFLSSACGGGGSCAQCKCQVIDGGGEILPTETVH
                     FSRKEVKDNWRLGCQVKVKNNMQIKIDEAILGVKQWECEVVSNNNVATFIKEFVVKLP
                     EGENLNFISGGYIQINIPKYAIKFSDFDIQDRFRGDWDKFNMWNLTCTNTEETVRAYS
                     MANYPAEGNIVMLNIRIATPPFDRINGGFLNVPPGIASSYIFSLKPGDKVIVSGPYGE
                     FHPLLDTGREMLYIGGGAGMAPLRSHILHLLNTLKIKDRKISYWYGARSKNEIFYEED
                     FRALEKEFPNFKFNIALSDPQPEDNWTGYVGFIHSVILNEYLSKHEAPEDIEYYMCGP
                     GPMANAVEKMLYDLGVPREMLMFDDFGA"
     gene            3146..>3589
                     /locus_tag="PLD76_04325"
     CDS             3146..>3589
                     /locus_tag="PLD76_04325"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27263.1"
                     /translation="MSSNTIQMFVEVILPVNISSTFTYSVPANWQGKLTKGMRVVVPF
                     GKNKIYTGVVLEILSSHNGEFVLKEIIEILDDNQFVSNAQLAFWKWIASYYMCSLGEI
                     MVAAIPSVFRLESQSIIRLHPDFDERETYSADFSSKTQESIFQYII"
BASE COUNT         1084 a          513 c          711 g         1281 t
ORIGIN      
        1 agatggagaa actaaatata ttttccctct ttatggtgct ggtttatggg gaccaatttg
       61 gggatatatt gccttaaatc atgataaaaa taccgtgtat ggagtgtatt tctctcatgc
      121 aggagaaact ccaggcttag gagctgagat aacatctcat gtgttccaat cacaatttcc
      181 aaacaaaaag ataaaaaata ccacaaacga tttggtgtca atagccattg taaaaccggg
      241 gaaaacggca gaaggtcaag attatgtgga tggtatttct ggtggaacaa tcacatctca
      301 tggggtagag cttatgttaa agaatacatt agtaaactat acaagttttt taaataaata
      361 attagcacat agcatctttt ctttttagag agtagatgtg aaaattaaaa taataagcgt
      421 atgaaaagtc tgttttctgc aaaaaataaa gaggttattg taggaccatt gagtttgaat
      481 aatccaatta ctgtacaagt gttaggtata tgttcttcct tagctgtaac agcaaaactc
      541 gagcctgcta ttgtaatggg aatgtctgta actattattg ttgccatatc aaatgttatt
      601 atttctttca tgcgaaatac cattcctgtg cgtattcgca ttatagtaca acttgttgtt
      661 gtagctacct tagtaactgt cgtaagtcaa tttctaaagg cttttgccta tgatgtaagt
      721 gtacagttgt ccgtatatgt aggattaatt attactaact gtatattgat gggacgttta
      781 gaagcatttg ctatgtctaa tagaccctgg gaatcttttt tagatggatt gggtaatggg
      841 tttggttatg cattaatttt ggttattgta gccttctttc gcgaattgct tggttctggt
      901 tcattacttg gattccatgt tatccctgca agtgtttacg aatcgggtta tatcaataat
      961 ggtttaatgg taatgcctcc aatggcactg attattttag ctattattat ttgggctcat
     1021 cgtacaaaag taaaacaatt acaagataat taattacaac ctagtaaatt cagaacgaat
     1081 ggaaaattta tcaagcttat ttgtcaagtc gatttttgtc gataatatga tttttgccta
     1141 tttcttggga atgtgttctt ttttagccgt ttctaagaat gttaaaactg cttttggtct
     1201 aggtgtagct gtaacgtttg tgcaggttat gaccttacct gttaactatt tattggagaa
     1261 ctatatactt aaacccgatg ttttaatcga aggggtagat ttgagttttt taagttttat
     1321 cgtctttatt gctgttatcg cgggtattac acaattggta gaaatgatgg tggagaaatt
     1381 tactcctgct ctatatgctt ctttaggaat tttcttacct ttaattgctg taaactgtgc
     1441 tatcatgggt ggctctttgt ttatgcaaca acgtaatttt acttccatta gcgaagcatc
     1501 tgtttttgca gtaggttcgg ggttaggttt ccttgttgca attgttggat tagctgcaat
     1561 tagagaaaaa atggcatatt cacatgtacc agcacctcta aaaggcttgg gaattacatt
     1621 tatcgtagta gggttgatgg cgatggcatt catgtgtttt tctggcttaa aagtctaata
     1681 aatttaagat acaaaacagc atttaaaaaa ataactatat gacattttta tctgtaagtc
     1741 ctattatgat aggcactagt atagtggtat ttttagcggt aattctttta ctcgtagtaa
     1801 tcttattatt tgctaaaaaa tatttatctc catcaggtcc tgttaaaatt ttaattaacg
     1861 gagaaaaaga gatagaagtt gaatctggat ctacattatt gtctacattg gcaaatgaaa
     1921 agatattttt atcatctgcg tgtggaggag gtggtagctg tgctcagtgt aaatgtcagg
     1981 ttattgatgg tggtggtgaa attttaccaa cagaaacagt tcacttttct cgtaaagaag
     2041 taaaagataa ttggcgttta ggttgtcaag taaaggtaaa aaacaacatg caaatcaaaa
     2101 ttgatgaagc tatcttagga gtgaagcagt gggaatgcga ggttgtttct aacaataacg
     2161 tggctacttt tatcaaagaa tttgttgtta aattgcccga aggagaaaat ttaaacttta
     2221 tttctggagg ttatattcaa attaatatac ccaaatacgc gattaagttt tctgattttg
     2281 atattcaaga tagatttcgc ggtgattggg ataaatttaa tatgtggaat ttaacatgta
     2341 ccaatactga ggaaacagta cgtgcgtatt ctatggcaaa ttatcctgct gaaggaaata
     2401 ttgtgatgtt aaatattcgt attgctacac caccttttga tagaattaat ggaggttttt
     2461 tgaatgtgcc tcctggtatt gcgtcatcgt atatcttttc actaaaacct ggcgataaag
     2521 taattgtttc aggtccttat ggagagtttc atcctctact tgacactggt agggaaatgt
     2581 tatatatcgg tggaggtgct ggtatggctc ctttacgttc tcacattttg catcttctta
     2641 atacgttgaa aattaaggat agaaagattt cttattggta tggtgctcgt tctaaaaacg
     2701 aaatattcta cgaagaagat tttcgagcat tagagaaaga attccctaat ttcaaattta
     2761 atattgcatt aagtgatcct caacctgaag acaattggac aggttatgtc ggatttattc
     2821 atagtgtgat attgaatgag tacctaagta aacatgaggc tccagaagat attgagtatt
     2881 acatgtgtgg acctggacca atggcaaatg ctgttgaaaa aatgttatac gacttaggtg
     2941 ttcctcgcga aatgctaatg ttcgatgatt ttggtgctta aataattgaa cgcatatttt
     3001 ttactaagag gctatcgtaa agatagtctc ttttgttttt aactcatttt attatttcat
     3061 atctgttatg ttgaaggtat atagcaaaca aaaatctttt ttccacgtag tttcttttct
     3121 tatctttgta ataaattcgt aaactatgtc ttcgaatact atccagatgt ttgtggaagt
     3181 aattcttcct gtcaatatct cttcgacttt tacctattcg gttccagcta actggcaagg
     3241 taaacttacg aaagggatga gggttgtcgt gccatttgga aaaaataaaa tttatacagg
     3301 cgttgttcta gagatactct cttcgcataa tggtgaattt gtactaaaag aaataattga
     3361 gattctagac gataatcaat ttgttagcaa tgctcaactg gcattttgga aatggattgc
     3421 gtcgtattat atgtgctcgt tgggcgaaat catggtggct gctattcctt cggtttttcg
     3481 attagaaagc caatcaatca tacgattgca tcctgatttt gatgaaagag aaacgtacag
     3541 tgctgatttt tcttctaaaa cgcaagaatc tattttccag tatattata
//
LOCUS       DAOSMY010000152         3576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26177_length_3576_cov_3.381710, whole genome shotgun sequence.
ACCESSION   DAOSMY010000152 DAOSMY010000000
VERSION     DAOSMY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26177_length_3576_cov_3.381710"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /gene="priA"
                     /locus_tag="PLD76_04330"
     CDS             <1..1946
                     /gene="priA"
                     /locus_tag="PLD76_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU27264.1"
                     /translation="MLTITHEVTEKFLPKYVTYVRLTWIAQHKIDSIFEEIKRAKKQV
                     HLLETFIDIAQCDDSTKPFPIEKSVLLQRASVSEAVLSELVKKQVLEVYKLPISRFQE
                     TSSLTENKLVLSPDQERAYKEIVGYFETKKPVLLHGVTSSGKTEVYIQLIQDAVHKDK
                     QVLYLVPEIALTIQLLQRLEQAFGNKVGVFHSKYSDQERAEVYQNLLSPNPYKIIIGV
                     RSSIFLPFQNLGLIIVDEEHEQSYKQVDPAPRYHARNAAIMLASIIQSNVLLGTATPA
                     IETYYNVSVKKYAYVSLASRYQNVLLPVIRVVDVADAYKKNRMKSMFSWFLIEKIKEA
                     IEQNQQVVLFQNRRGFSSHVECQQCGWVPKCMACDVSLTYHKFANYLSCHYCGYTEQL
                     PTQCPQCGNNQLKDRGFGTEKIEEELGKLFPGIRTARMDVDTTSKKNAYKKLIQAFEN
                     HQIDVLIGTQMITKGLHFDNVSLVGILNADNLFNYPDFRANERAFQMLVQVSGRAGRK
                     QKQGTVVLQTFSPHNPIVNYIVNNDYHGFFCSETEERKVFNYPPFCRLIHIFVKDSDK
                     EIVDRVVNELACHLRRSFGNRVLGPDNPPVSRIQHIYIKKIIVKIELQSSFEKAKEKV
                     QNSIASLLHQYKKTRIVIDIDPL"
     gene            2061..2528
                     /locus_tag="PLD76_04335"
     CDS             2061..2528
                     /locus_tag="PLD76_04335"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOU27265.1"
                     /translation="MKKILFVCLGNICRSAAAEAVMKSVVNKIGRDKDFFIDSAGIIS
                     VHKGEPADNRMRKHAARRGYAVTSISRPVNYSDFEQFDIIVGMDNTNIDDLKDKAPSV
                     EAQKKIYKMTDFCERSQYDYVPDPYYGGQDGFELVLDILEDACDGLLKKIDVL"
     gene            2563..3321
                     /locus_tag="PLD76_04340"
     CDS             2563..3321
                     /locus_tag="PLD76_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27266.1"
                     /translation="MKENIVTLLYFLGIMGAILMYFVFPNDITHAILFGAICLIITII
                     PALLLSRTKKKKKPIYTYTDESFADFHPIQSDSLSMIEEVSNTTRKVSDSSLTAKRMY
                     GKVLAYVADFEELNKAPLSAEGRTECVLFVSTVVLLEYKSLYQIEEYRDFEVAYLDIM
                     LSLVHQNATQKSKEEVYEFVKRRLRFYTAEYQTLIDNKFPLEFYSYFFYTPLRTRTQV
                     CLDQNELIRFHGNLLSLVEKIKLSTKNDASFLGV"
     gene            complement(3310..>3576)
                     /locus_tag="PLD76_04345"
     CDS             complement(3310..>3576)
                     /locus_tag="PLD76_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU27267.1"
                     /translation="PNELSGGEKQRVAVARALINNPSIILADEPSGSLDSRNKEELHQ
                     LLVTLNRDLGITLLVVTHDTNLAYLSNRIIEMQDGIIIGEKLNA"
BASE COUNT         1205 a          515 c          684 g         1172 t
ORIGIN      
        1 atatgttgac gattacccat gaagtaaccg aaaaatttct accaaaatat gttacgtatg
       61 tgcgattaac atggatagct caacataaga tagactcaat ttttgaagaa ataaaacggg
      121 caaaaaaaca agtgcacttg ctcgaaacct ttatagatat cgctcaatgt gatgatagca
      181 caaagccctt ccctatagaa aagtctgttt tgcttcaacg agcttctgtt tcagaagctg
      241 tgctatccga attggtaaaa aaacaggtat tagaagtata taaattacct atttcacgtt
      301 ttcaagagac ttcatcatta accgaaaata agcttgtatt atcgcccgat caagaaagag
      361 catacaaaga gattgttggg tattttgaaa cgaaaaaacc agtattatta catggagtta
      421 cctcaagtgg aaaaacagag gtatatattc aattaataca agatgctgta cataaagata
      481 aacaagtgtt gtatttagtg ccagaaatag cgttgactat acaattatta caacgtttgg
      541 agcaagcttt tggtaacaag gtaggggtgt ttcattcaaa atattctgat caagaacgtg
      601 cggaggtata tcaaaattta ctgtcgccaa acccctataa aattattatt ggagttcgtt
      661 catctatttt tttgcctttt caaaatcttg gtcttattat tgtagatgaa gaacacgaac
      721 aatcttataa acaagtagat ccagctccac gataccatgc tcggaatgct gcaattatgt
      781 tggcttctat tatccagagt aatgtattac tgggtactgc cactccagct attgaaacat
      841 attacaatgt aagtgtaaaa aaatatgcct atgtgtctct tgcttcgagg tatcaaaatg
      901 tattgttgcc agtaattcgt gttgttgatg tagcagatgc gtataaaaag aatagaatga
      961 aatcgatgtt ttcttggttc ttaattgaaa aaattaaaga ggctattgaa caaaatcagc
     1021 aggtggtact gtttcagaat aggagaggat tttcatccca tgttgaatgt caacaatgtg
     1081 gttgggtgcc aaagtgtatg gcatgtgatg taagtcttac atatcacaaa tttgctaatt
     1141 atttatcgtg tcattattgt ggttacacgg aacagttgcc aactcaatgt ccccagtgtg
     1201 gaaataatca gttgaaagat agaggttttg gtacagaaaa aattgaagag gaattaggta
     1261 aattattccc aggtattcga actgctcgga tggatgtaga tactacttcg aaaaaaaatg
     1321 cttacaagaa acttattcag gcgtttgaaa atcatcaaat agatgtgctt atcgggacac
     1381 aaatgataac gaagggtttg cattttgata atgttagttt agtaggaatt ttaaatgcag
     1441 ataatttgtt taattatcct gattttagag ccaacgaaag ggcttttcag atgcttgttc
     1501 aagtaagcgg tagagctgga aggaaacaaa aacagggaac tgtagtactt caaacatttt
     1561 ctccacacaa tccgattgta aattatattg taaataatga ttatcatggt tttttttgtt
     1621 ccgaaaccga agagcggaaa gtatttaatt atccaccatt ttgcagatta atacacatct
     1681 ttgttaaaga tagtgataag gagatagtag atagagtggt aaacgaacta gcttgtcatt
     1741 tacgccgtag ttttggcaat agggttttag gtccagataa tccgcctgta tcaagaattc
     1801 aacacatata cattaaaaaa attattgtaa agatagagtt gcaaagttcg tttgaaaaag
     1861 caaaggaaaa ggtgcaaaat agtatagcgt cacttttaca tcaatataaa aaaactagaa
     1921 ttgttattga tatagatcct ttatagtgat ataacgaatt aagcgaatga taaagatttt
     1981 gtttaaaatt gttataattc agtattattc cttatttttg cagtagcaat ctctatctct
     2041 gtaaaaagtt agtatacaaa atgaaaaaaa ttttatttgt atgtttgggt aatatctgtc
     2101 gttctgctgc cgctgaagct gtaatgaaat ctgttgtaaa caaaattgga agagataaag
     2161 atttttttat tgattcggca ggtataatat cagtgcataa aggagagccc gcagataatc
     2221 gaatgcgtaa acacgcagcc cgccgggggt atgctgtaac aagtatttct agaccagtta
     2281 attatagcga ttttgaacaa tttgatataa ttgttggtat ggataatacc aatatagatg
     2341 atttgaaaga taaagctcca tcagtggagg cacaaaagaa gatttataaa atgactgatt
     2401 tttgtgaacg ttctcaatat gattatgtgc cagatccata ttatggagga caagatggat
     2461 ttgaattagt attagatatt ctggaagatg cttgtgatgg attattgaaa aaaatagatg
     2521 tgttataggg tgcagagtat ataactttat atagggaaat aaatgaaaga aaatattgtt
     2581 actttgttgt attttttagg cataatggga gcgattttaa tgtattttgt ttttcctaat
     2641 gatattacgc atgctatttt attcggagct atatgtttaa ttattaccat tataccagca
     2701 ttattgctta gtagaactaa gaaaaagaag aagcctatat acacctatac agatgaatct
     2761 tttgctgatt ttcaccctat acagtccgat tcattaagta tgattgaaga ggtttctaat
     2821 acaactcgca aagtctctga tagttctctt acagcaaaaa gaatgtatgg gaaagtgctt
     2881 gcttatgtag ccgattttga agagttaaat aaagctccac tgagtgcaga aggtcgtacg
     2941 gaatgtgtgc tttttgtgtc aacggtagtg ttgctcgaat ataaatcctt gtatcaaata
     3001 gaggaatacc gtgattttga agtggcatat ttggatatta tgctttctct tgtacaccaa
     3061 aatgcaacac aaaaatcgaa agaagaagtg tatgaatttg taaaacgtag attgcgtttt
     3121 tatacagccg aatatcaaac tcttatagat aataaattcc ctctggagtt ttattcgtac
     3181 tttttttata cccctttacg tacacgtaca caagtttgcc ttgatcagaa tgaattaatt
     3241 cgttttcatg gaaatttatt gtctttggtc gaaaaaatta agctgtcaac aaaaaatgat
     3301 gcaagctttt taggcgttta gcttttctcc aataataatg ccatcttgca tctcaattat
     3361 tctgtttgac agataagcta agtttgtgtc atgtgttact actaataggg tgattcctaa
     3421 atctctgttt aatgttacta aaagttgatg taactcttct ttatttcttg aatctaaact
     3481 gcccgaaggt tcatccgcaa gaataatgga ggggttgtta attaaagctc ttgcaacagc
     3541 aacacgttgt ttctctcctc ctgatagttc gttggg
//
LOCUS       DAOSMY010000153         3564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26298_length_3564_cov_3.256768, whole genome shotgun sequence.
ACCESSION   DAOSMY010000153 DAOSMY010000000
VERSION     DAOSMY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26298_length_3564_cov_3.256768"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLD76_04350"
     CDS             <1..768
                     /locus_tag="PLD76_04350"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU27268.1"
                     /translation="KHLFTPENVVDIQRIIGADIIMALDECTPGDADYAYAKKSMELT
                     HRWLARGIQHFDTTEPKYGYSQTFFPIVQGCVYPDLRKQSAEFVASQGREGNAIGGLA
                     VGEPTEKMYEMIEIVNEILPTDKPRYLMGVGTPANLLEAIERGVDMFDCVMPTRNGRN
                     GMLFTKNGIINMRNKKWADDFSPIEIDGASYVDTAYSKAYLRHLFISNELLGLQIASI
                     HNLAFYLWLVQEARKQILAGTFSVWKTNMIQQVSQRL"
     gene            894..2759
                     /locus_tag="PLD76_04355"
     CDS             894..2759
                     /locus_tag="PLD76_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOU27269.1"
                     /translation="MKTHFHLLLFFFVCLHFGVHAENAKQGVSVWKIDPHSGDKEFIE
                     VDTIALNYHSKTNPVYNHTIATSWLGNLGLPAESKIYFDKNQGYVTHSFLFKNTYASY
                     FLSPENISFYNTKTPFSNVSYHTGGFAYREEDNISGLFTVNATPKLNFSLLANHIYGR
                     GAYQNQATDGLSGALYGSYLGDKYGIHFITALNNLKHFENGGIADISQLTSTIESYNL
                     PVNLSNAWSIFHSFYLYASQQYHLGFHTNDNKFVPVTTFGHSISFEQNQKKYYEKSIS
                     DFYQNSYYSTTSTNDTVAFNVLKNTAYISLNEGFHKWALMGVRAFIDFDIEQNTDLTA
                     DWSLRKKTTSLVYAGGDISKTKGNTTYHALGKMMLLGDARGEYELNGKLNTQLKLGSQ
                     NIQLQGKALLVSSNPYHHLEHYYSNHFMWERDYENIVRTQCSGSISLPNKVANITLKA
                     GVENTTNYIYFDTNALPQQYSNAIQIVHGEATLNLHLGNIHFENTAIFQQSSNEAILP
                     LPSLSSYHNLYVTHTLLNVLKFQIGADCRYHTAYYANAYMPATGQFYRQTETLIGNYP
                     FINLYANFHLKRTRFFVMYYNLSEAIFTDKNYTLVPNYPLNPGMVKFGLSWNFYD"
     gene            2805..>3564
                     /locus_tag="PLD76_04360"
     CDS             2805..>3564
                     /locus_tag="PLD76_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU27270.1"
                     /translation="MKLCSKISLVFVILVISFTVLFAFLRHSSPLFSTDYKDIIASGT
                     LRVVIEKNTMSYVVVNDTNVMGLQYKMVEQLAKDWGVTVQYIDASDLGSAINMLNNNE
                     VDIVVWHIPIHVEMEDKIAFTVPVFTSRQVLVQRKNTPHTLIRNQLDLARKTIHIPKD
                     SPYKQRIQNLAKEIGDSIFIVEINEISIDSLFYKVANAEIDFTVCEKVVASIEKKKYP
                     TLDTETAIGFKQNYAWAIRQNSAELQDSVNNWLNY"
BASE COUNT         1254 a          656 c          610 g         1044 t
ORIGIN      
        1 aaacatttgt ttacgcccga aaatgtggta gatattcagc gtattatcgg agcagacatt
       61 attatggctc ttgacgaatg taccccaggt gatgccgatt atgcgtatgc aaaaaaatca
      121 atggaactta cgcatcgatg gttggcacgt ggtatacaac attttgacac tactgaaccg
      181 aaatatgggt atagccaaac attttttccc attgtgcaag gatgcgtata tccagatttg
      241 cgcaaacagt cggcagaatt tgtagcctcg caaggcagag aaggaaatgc tattggaggc
      301 ttggcagtag gcgaacctac cgaaaaaatg tatgaaatga ttgaaatagt aaacgagata
      361 ctgcctacag ataaacctcg ttacctaatg ggtgttggta cacctgccaa cctattggaa
      421 gctatcgaac gaggggtaga tatgtttgac tgtgtaatgc ctacacgcaa tggtagaaac
      481 ggtatgcttt ttacaaaaaa tggaattata aatatgcgta ataaaaaatg ggcagacgat
      541 ttttctccta tcgaaataga tggagcatcg tatgtagata ccgcctactc taaagcctat
      601 ttgcgtcatt tgtttatatc caacgaattg ttaggattgc aaattgcatc cattcacaat
      661 ctagcttttt atttatggtt agtgcaagag gcacgcaaac aaatacttgc aggcactttt
      721 tcagtatgga aaacaaacat gatacaacag gtatcacaac gtttataaaa tatttttgtt
      781 attttaaaga ctaaatagta cttttgacac tataaaaaga gtatattttc agaggattaa
      841 acacacagat accaagctat ctttaacatt gatcaataat acctatttat tgcatgaaaa
      901 cacacttcca tcttcttttg ttttttttcg tgtgcttgca ttttggagtg catgcagaaa
      961 atgcaaaaca aggagtttct gtttggaaaa ttgatccaca tagtggagat aaagaattca
     1021 tagaggtaga cacaatagca cttaattatc attcaaaaac aaatccagta tataaccaca
     1081 ctatagcaac atcgtggcta ggaaacttag gactacctgc tgaatcaaaa atttattttg
     1141 acaaaaatca aggctacgta acccattcat ttttgtttaa aaatacgtat gcatcctatt
     1201 tcctttcgcc cgaaaatatt tcattttaca atacaaaaac accttttagt aatgtaagct
     1261 atcatacagg aggttttgct tacagagaag aagacaatat ttcgggatta tttacagtaa
     1321 acgctacacc taaacttaat ttttcgttac tagccaacca tatatacgga cgaggagcct
     1381 atcaaaacca agcaacagac ggacttagcg gagcattgta tggtagctat ttaggagaca
     1441 aatatggcat acattttatc acagcactta acaacctaaa acattttgaa aatggaggta
     1501 ttgctgacat aagccaacta acgagtacga tagaaagtta taatctacct gtcaatttat
     1561 ccaatgcatg gtctattttc cattcatttt atttgtatgc atcacagcaa taccatcttg
     1621 gatttcacac taacgacaac aaatttgttc ctgttactac ctttggtcat agtatatcgt
     1681 ttgaacaaaa ccaaaagaaa tactacgaaa aaagcatatc tgatttttac caaaactcat
     1741 actacagtac cactagcacc aacgacactg tggctttcaa tgtattaaaa aatacagcat
     1801 atatatctct aaatgagggt tttcataagt gggcattaat gggagtacgt gcttttattg
     1861 atttcgatat agaacaaaat actgacttaa cagctgattg gagtttacgc aaaaaaacta
     1921 cgagcctagt atatgcagga ggagacattt caaaaacaaa aggcaacaca acctatcatg
     1981 ctttaggcaa aatgatgcta ttgggcgatg cacgtggaga atatgaatta aatggcaaat
     2041 tgaatacaca attgaaattg ggttcgcaaa acatacagtt acaaggcaag gctcttttag
     2101 taagcagtaa tccgtaccat catcttgagc actattattc caatcatttt atgtgggaaa
     2161 gggattacga aaatattgtt cgcacccaat gtagtggtag cattagtttg ccaaacaaag
     2221 tagcaaatat aacactcaaa gcgggagtag aaaataccac taattacatc tattttgaca
     2281 ccaatgcttt gcctcaacaa tacagcaatg ccatacaaat tgtgcatggc gaggcaacac
     2341 taaacctgca tctaggcaat attcatttcg aaaatactgc tattttccaa caaagttcaa
     2401 atgaggctat tttaccctta ccaagcctat cttcttatca taatctgtat gttacgcata
     2461 cattactcaa cgtactcaaa tttcaaatag gagcagattg tcgttatcac acagcttatt
     2521 atgccaatgc atatatgcct gctactgggc aattttaccg ccaaacagaa acccttatag
     2581 gtaactaccc atttataaac ctatatgcca actttcatct caaacgaaca cgcttttttg
     2641 taatgtacta caatctttcg gaagcaattt ttacggacaa aaattacacc ttagtaccta
     2701 attacccatt aaaccctgga atggttaaat ttggcttatc gtggaatttt tatgattaat
     2761 ttgagtatct ttgtgtataa cttacttaaa aaaacaaatg tcctatgaaa ttatgttcaa
     2821 aaatatcatt ggtattcgtc attctagtta tttcttttac agtacttttt gctttcttgc
     2881 gacactcatc tccgctattc tcaacagact ataaagatat catagcatca ggcaccctga
     2941 gggtagtgat agaaaaaaac acaatgagct atgtggtggt aaacgatacc aacgtaatgg
     3001 gtttgcaata caaaatggta gaacaattag ccaaagattg gggggtaact gtgcaataca
     3061 tagatgcctc tgacttaggt tcggctatta atatgctcaa taacaacgaa gtagatatcg
     3121 tagtatggca tattccaata catgtagaaa tggaagataa aattgcattt acagtacctg
     3181 tttttacaag cagacaagta ttggttcagc gaaaaaacac gccacataca cttattcgta
     3241 accaattaga cttagcaaga aaaaccatac acatccccaa agattccccc tataaacaac
     3301 gtattcaaaa tttggcaaaa gaaattggag acagcatttt tatagtagaa ataaatgaaa
     3361 taagcattga ttcgctgttt tataaagtag ccaatgcgga aattgatttt actgtatgcg
     3421 aaaaagtagt tgcatctatt gagaaaaaaa aataccccac actggatacc gaaacagcta
     3481 tcggatttaa gcaaaattat gcatgggcta tacgtcaaaa ctcagcagag ctacaagata
     3541 gtgtaaataa ttggctaaac tatt
//
LOCUS       DAOSMY010000154         3524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26684_length_3524_cov_3.480830, whole genome shotgun sequence.
ACCESSION   DAOSMY010000154 DAOSMY010000000
VERSION     DAOSMY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26684_length_3524_cov_3.480830"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2758)
                     /locus_tag="PLD76_04365"
     CDS             complement(<1..2758)
                     /locus_tag="PLD76_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27271.1"
                     /translation="MLATLLLNTKPVQKYLLHQATHYLSNKIGSEVSISTVTYNLFTT
                     FEFSDIQIKDKERNDLLTIEHIDIKFKILPLFKQKLIVKSVSFIQPSINILRDSAGIS
                     NFQYILDAFAKNETDSIESKFVFDIRNITIKKGELKLTNIGTARKTQTFDPNHIHIWD
                     LNSEMRLDKIKSDSIVFELKSCNFEEQSGLIVKNLYADASFGKEYIHINDLSIKLPSS
                     IMEFEKLSIDYAHQENINSIVHMLDKSSIVLKSKKVVITGRDIACFVPRLAHLKNPIN
                     IEVNAQGANNTIELSKCIIKYGDQISLDASMQLAGINNMDETFIYADIHSISAHSTAI
                     ETVVSDIMQKPFILPKEIRNLGQMHYTGNIAGFFNNLVVYGKLRTDIGILSTDILLGI
                     DIQKNDIAYSGNLRSSRFQLTKMLGKDSGFGNIQFRVDVNGTKTHENKHNGIVKGNVQ
                     SIDFNKYTYKNIKLDGEYNNNGFNGKIELNDDNGQIKFDGDIDFSRKIPLMNFVAQVN
                     NVSPYKLQLTEHNPDLSLSFTIASSIQGTTIDNVIGNVFIQDINLHNASDTFEIKQVE
                     MKSFIEDEKQCLRITSDFLNATILGKITLSSIANNFLHIVHTEVPNISSLQRDTKLSG
                     NNFTFEANIEPIDQLSKVLLWDISTKEPTFIQGSFNDFTNIFSINAQSKGIQLNKNYL
                     YFATIDLHNSTSGIDLDANVGLKMPKDSLLVNVTSKILNNQGNTSISWKNSYGKEFNG
                     KLESVLDIKKNEDNEVETNIHILPTEMVVNDSIWHITNSTLVYKKERLLFDKFAITQH
                     DKFIRINGIASKLEDDWLYVDINQFNLEYLSELLQLKGIHLSGIATGSITAQQAFNKP
                     ILNASINATDFGLNSAPFGNVEVTANYNYEKEQIDIEGLITNHKLQQSHVSGYISPVR
                     NDMLL"
     gene            2940..3524
                     /gene="tsaD"
                     /locus_tag="PLD76_04370"
                     /pseudo
     CDS             2940..3524
                     /gene="tsaD"
                     /locus_tag="PLD76_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412030.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
BASE COUNT         1085 a          552 c          558 g         1329 t
ORIGIN      
        1 gtaaaagcat atcattgcgt acaggagata tgtatccact tacatgagat tgttgaagtt
       61 tgtggtttgt tatcaatcct tcgatatcta tttgctcttt ttcgtaatta tagtttgcgg
      121 ttacttctac attgccaaaa ggggcactat ttaaaccaaa atctgtagca ttgatagatg
      181 catttagtat tggtttattg aaagcttgct gtgcagtaat gcttccagtt gcaataccag
      241 acagatgtat gcctttaagt tggagtagct cacttaaata ttctagatta aattggttga
      301 tatccacata caaccaatca tcttctaatt ttgatgctat tccatttatt cgaataaact
      361 tatcgtgctg tgtaatagca aatttatcaa acaataaacg ttctttttta tacactaatg
      421 tactgtttgt aatatgccat attgaatcat ttaccaccat ttctgtaggt aaaatgtgaa
      481 tattcgtttc tacctcgtta tcttcatttt ttttgatatc aagtacactt tctagtttac
      541 cattaaattc tttgccatag ctatttttcc aagaaataga tgtatttcct tgattattaa
      601 gaattttgct tgttacattg actaataaac tatcttttgg catttttaat cctacattgg
      661 catcaaggtc gatacccgaa gtactattat gtaaatcaat cgtagcaaaa taaagatagt
      721 tcttgtttaa ttgtattcct ttgctttgtg cgttgataga aaaaatattt gtaaaatcat
      781 taaaagaacc ctgtataaaa gttggttctt ttgtgctaat atcccataat aatacttttg
      841 ataattgatc tattggctca atgttagcct caaatgtaaa attatttcca cttaattttg
      901 tatcccgttg taaggaactt atatttggca cttcggtgtg tactatatgc agaaaattat
      961 tcgcaataga cgatagtgta attttaccaa gaattgttgc attcaaaaag tcagatgtta
     1021 ttcgcaaaca ttgtttttca tcctcgatga acgacttcat ttctacttgt tttatttcaa
     1081 atgtatcgct tgcattatgg aggttaatgt cctgtataaa tacattacca ataacattat
     1141 caatagttgt accttgtata gaggaagcaa ttgtaaacga aagactcaag tctggattat
     1201 gttcagtaag ttgcaatttg tacggactca cattattcac ttgggcaaca aaattcatca
     1261 aaggaatttt tcgagaaaaa tcaatgtcac catcaaattt tatttgtccg ttatcatcgt
     1321 ttaattcaat ttttccatta aacccattgt tattgtattc tccatctaat ttgatatttt
     1381 tatacgtata tttgttgaaa tctattgatt gtacattccc ttttacaata ccattgtgtt
     1441 tgttttcatg tgttttggtg ccatttacat ctaccctaaa ttgaatattt ccaaaaccag
     1501 aatctttacc tagcattttt gttagttgaa aacgagaaga acgcaaattt ccactatatg
     1561 caatgtcatt tttttgtata tcaataccta ataatatatc cgtggataat atgcctatat
     1621 ctgtacgcag ttttccatat accaccaaat tgttaaaaaa tccagcaata tttccagtat
     1681 aatgcatctg tcccaaattc cgtatttctt ttggtagtat aaatggtttt tgcataatat
     1741 ctgaaaccac tgtttctatt gccgtactat gtgcagagat tgaatgaatg tcggcatata
     1801 taaatgtttc atccatgtta ttaataccag ccaattgcat tgatgcgtcc aaagatattt
     1861 gatctccata tttaataata catttgctca gttctatggt gttgttagct ccttgtgcat
     1921 ttacttctat gtttatcgga ttttttaaat gtgctaagcg aggaacaaaa caggcaatat
     1981 ctctacctgt tataactact ttctttgatt tgagcacaat agaagatttg tctagcatgt
     2041 ggacaataga attgatattt tcttgatgag cataatcaat agaaagtttt tcaaattcca
     2101 taatggaaga aggtagctta atagataaat cattgatatg gatatattct ttaccaaacg
     2161 aagcatcagc atataagttt tttacaatta atccagattg ttcttcaaaa ttacatgatt
     2221 tcaattcaaa aacgatagaa tccgatttaa ttttgtctaa acgcatttct gagttcaaat
     2281 cccaaatgtg aatatgatta ggatcaaaag tttgggtttt gcgtgcggta cctatattcg
     2341 ttagttttag ttctcccttt tttattgtta tatttctgat atcgaataca aacttactct
     2401 caatactgtc tgtttcattt ttagcaaaag catccagtat gtattgaaag ttactaatcc
     2461 ccgcactatc tcttagtata ttgatagatg gttgaatgaa agagactgat ttgacaatta
     2521 atttttgttt aaataaggga agtattttaa acttaatgtc tatatgttca atagttagta
     2581 ggtcattacg ttctttatct tttatctgaa tatctgaaaa ttcaaatgtc gtaaataaat
     2641 tatatgttac agttgaaata gaaacttcgc ttccaatttt gttggaaagg taatgggtgg
     2701 cttgatgcaa caaatatttt tgtactggtt tggtattgag taataaagtt gctagcaaaa
     2761 gtagtcctgc tacaattgct aacgtaatac ttgttatagt tacatatttt ttaataactt
     2821 tgcactttaa tttcaaccta caaaaataac aataaaacct gttaaataaa atagaaaacc
     2881 aataagcagt agatattttg tttttttaag gataatttga gtttcttttt ctgtaattta
     2941 tggataatat tactatactt ggaattgaat catcgtgcga tgatacttct gctgctgtta
     3001 ttcgtaacgg ggaattgttg tctaatgtta ttgctaatca agatgtacat attgcttatg
     3061 gtggggtagt tccagaatta gcttcaagag cacatcaact caatattgta ccaaccatac
     3121 atcaagctat taaaaatgcc tcggtttcgt taagtgagat acatgccata gcctatactc
     3181 gtggaccagg gctcatgggc tctttactcg taggtacttc ttttgcaaaa ggattagcat
     3241 tgtcattagg agttccttta attgatgtaa atcatttaaa agcacatgtg ctggttcatt
     3301 ttattcatga taatactcca aattttaagc caccaacttt ccctttttta tgtttgttgg
     3361 tgtcaggggt aattcacaaa ttgtactggt gaaatcatat agcgagatgg aaattatagg
     3421 acaaaccatt gatgatgctg ctggagaagc ttttgataag tgtgccaaaa tacttggcgg
     3481 accttatccc ggtggtccgt ttgttgatgt gatggctcag caag
//
LOCUS       DAOSMY010000155         3513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26796_length_3513_cov_3.601215, whole genome shotgun sequence.
ACCESSION   DAOSMY010000155 DAOSMY010000000
VERSION     DAOSMY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26796_length_3513_cov_3.601215"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLD76_04375"
     CDS             <1..305
                     /locus_tag="PLD76_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU27272.1"
                     /translation="ESNYDEQTLLENPSYPTYLKERILSNKGHLSNHALAEFLSINYQ
                     EHWQHIFLCHLSKENNSPECALSTIDKKIAHQRFNLWTNCTLIALPRTTPTEVFII"
     gene            527..2371
                     /locus_tag="PLD76_04380"
     CDS             527..2371
                     /locus_tag="PLD76_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU27273.1"
                     /translation="MAEQVKKDNQDNIVIRFSGDSGDGMQLAGTLFANISAMSGNDIS
                     TFPDFPSEIRAPQGTLSGVSGYQVHIGPKVYTPGDKTDVLIAMNPAALKINVKNLKNS
                     SVVVIDTDSFTKKDLEKALFSTSNPFEELGIGNISVIEAPIGSMVKESLTNSGLEGKD
                     VLRCKNMFALGLSCWLFDRPLNLAESLIRTKFAKKPIVAEANIKVLHDGYNFGANTHA
                     SVPTYHIATSTKEKGFYTDVDGNTATAYGLIAAAEKAGMELFLGSYPITPATDILQEL
                     AKHKALGVKTMQMEDEIAGICSAIGASFAGSLAATNTSGPGLALKSEAIGLAVMAELP
                     IVIIDVQRGGPSTGLPTKSEQTDLMQALYGRNGESPVAVVAAKSPTDCFDAAFWASKI
                     AVEHMTPVILLTDGYIANGSGAFKIPELENYPSIQPHYIKKDQQNWKPYTRNEETKVR
                     YWATPGTEGFQHRIGGLEKDYTTSIISTDPANHQLMVETRQEKINAIANFIPIIDIQG
                     DANADVLIVGWGGTYGHLLSAVNEMNENGKKTAFAHFSFISPLPKNTEEILRKYKKII
                     VCEQNLGQFAGYLRMKIHGLPIYQYNEVTGQPFLVSELVKYFTKIMEE"
     gene            2376..3398
                     /locus_tag="PLD76_04385"
     CDS             2376..3398
                     /locus_tag="PLD76_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU27274.1"
                     /translation="MSELTYKPFQASDYKSAQAVRWCPGCGDHFLLNSLHKAMANIGI
                     PPHQIAVISGIGCSSRLPYYMNTYGFHTIHGRGAAIASGLKVANPKLSVWQITGDGDC
                     LAIGGNHFIHSIRRNMDLNIVLVNNQVYGLTKGQYSPTSDRGYVCKSAPYGTVEDPFR
                     PAELTFGARGTFFARTVDVDSPVAVEVLTAAAKHKGASVVEVLENCIIFNDGTHSAVS
                     KKEGRAKNAIVLQHGKPMIFGENNEKGLILDGMNLKVVNLGENGVTEKDILIHDETTI
                     DFTLHLKLATMPFPEFPIALGVIRSIKAPTYDEEITVQIKDVQAKNPTRSLKDYLMKG
                     EIWEVK"
BASE COUNT         1236 a          624 c          651 g         1002 t
ORIGIN      
        1 tcgaatcaaa ttatgacgaa caaaccctat tggaaaatcc atcctatcca acatatctaa
       61 aagagcgtat tttaagcaac aaaggacatt tgagtaacca cgcattagca gaattcttat
      121 caattaatta ccaagaacat tggcaacaca tttttttgtg tcatttaagt aaagagaaca
      181 actcgcccga atgtgcacta tctaccatcg acaaaaaaat tgcccatcaa agatttaatt
      241 tatggactaa ttgtactcta atagctttac ctcgtactac ccctactgaa gtttttatta
      301 tttaaaagac ggttatagtt acttttcctt acatccaagc tatttttggc gtccgttttt
      361 gaaataattc tttgaaaaat gaattttata ccgtattttt gtgctcccaa aataccatga
      421 cagttcagat aaataaaata gtacgactac atcaatcaat atcagaagta ttcgtacgaa
      481 tacttaacaa taagtaatcc acgtaaaaaa gaatgaatac tatcctatgg cagaacaagt
      541 aaaaaaagac aatcaagaca atattgtcat ccgattttca ggagactctg gagatggaat
      601 gcaattggcg ggcacacttt ttgccaacat atcggcaatg agtggaaatg atatttctac
      661 atttcctgat tttccttccg agattagagc ccctcagggc acattaagcg gtgtatcagg
      721 ctaccaagta catataggac ccaaagtata tactccagga gataaaactg acgtactaat
      781 agcaatgaat cctgctgcat taaagataaa tgtaaaaaac ctgaaaaata gttctgttgt
      841 agttatcgac actgatagtt ttactaaaaa agatttagaa aaagcattat tttcaacgtc
      901 taaccccttt gaagaacttg gaataggaaa tatcagcgta attgaagctc ctataggatc
      961 tatggtaaaa gaaagcctta ctaattcagg attagaaggt aaagatgtgt tacgatgcaa
     1021 aaatatgttt gcattaggtt tatcgtgttg gttgtttgat cgcccattaa acttagcaga
     1081 gagcttaatt cgcacaaaat ttgcaaaaaa accaattgta gccgaagcaa acataaaggt
     1141 actacacgat ggatataatt ttggagctaa cacgcacgct tctgtgccta cttaccatat
     1201 tgctacaagt acaaaagaaa agggatttta cactgacgta gatggtaata ccgcaactgc
     1261 ttatggttta attgcagctg ccgaaaaagc gggcatggaa ttattcttag gaagctatcc
     1321 tattacacct gctaccgata ttttacaaga acttgctaaa cataaagcgt tgggagtaaa
     1381 gaccatgcaa atggaagatg aaatagcggg tatttgctca gctattggtg caagttttgc
     1441 tggttcgtta gctgctacaa acacttccgg accaggttta gctcttaaaa gcgaggctat
     1501 tggcttagct gttatggcag aattgccaat tgtaattata gatgttcaac ggggaggacc
     1561 ttcaacaggc ttgccaacca aaagcgaaca aacagactta atgcaagcgt tatacggtcg
     1621 caatggagaa agtccagtgg ctgtggtagc agcaaaatcg ccaacagact gtttcgatgc
     1681 tgctttttgg gcatccaaaa tagctgtaga acacatgacc ccagtaattt tactcactga
     1741 tggttatata gcaaatggtt ccggagcatt caaaattccc gaattagaaa actatcctag
     1801 tattcaacct cattatatca aaaaggatca acaaaactgg aaaccataca cacgtaacga
     1861 agagactaaa gtgcgttatt gggcaacacc tggaacagaa ggttttcagc atcgcattgg
     1921 tggattagaa aaagattata caacaagtat tatttcaaca gatccagcca atcaccaact
     1981 tatggtggaa acacgtcaag aaaaaattaa tgcaattgca aattttatcc ctattattga
     2041 tatacaagga gatgcaaacg ctgatgtatt aatagttggt tggggtggaa catacggaca
     2101 tctattatca gctgtaaatg aaatgaatga gaacggtaaa aaaacggcat ttgctcattt
     2161 tagttttatt agcccattac ctaaaaatac agaagaaata ctgcggaaat acaaaaaaat
     2221 aattgtttgc gaacaaaatc ttggtcagtt tgcaggttat ttacgcatga aaattcatgg
     2281 gttacctatc taccaataca acgaggtaac tgggcaacct tttttagtaa gcgaattagt
     2341 aaagtatttc acaaaaatta tggaggaata aaaatatgtc agaattaaca tataaaccat
     2401 ttcaagcatc cgattataaa agcgcacaag cagtacgctg gtgtccaggt tgtggcgacc
     2461 attttttatt aaattctcta cacaaagcaa tggctaacat tgggataccc ccgcatcaaa
     2521 tcgcagttat ttctggtata ggatgttctt ctcgattacc ttattatatg aatacctatg
     2581 gttttcatac cattcatggt cgtggtgctg ctattgcatc gggcttaaaa gttgccaatc
     2641 caaaattaag tgtatggcaa attactggtg atggcgattg tcttgccatt ggaggaaacc
     2701 attttattca ttccatacgt agaaatatgg atttaaatat tgtattagtt aataatcaag
     2761 tatatggctt aacaaaaggt cagtattccc ctacttctga tcgcggttac gtctgtaaat
     2821 cagctcccta tggaacagta gaagatcctt ttcgtcctgc tgaattaaca tttggtgcta
     2881 gaggtacttt ttttgcacgt acagtagacg tagattctcc tgtggcagta gaagtattaa
     2941 cggcagcagc caaacacaaa ggagcctcag tagtagaagt attagaaaat tgcatcattt
     3001 tcaatgatgg aacgcacagt gctgtaagta aaaaagaagg acgtgcaaaa aatgccattg
     3061 tattacaaca tggcaaacct atgatttttg gcgaaaataa cgaaaaagga ttaatcctag
     3121 atggtatgaa cctaaaagta gttaatttag gtgaaaatgg tgttacagaa aaagatattc
     3181 taatacatga tgaaacaacc atagatttta ccctacatct aaaactagcg actatgccat
     3241 ttcctgaatt tccgattgct ttaggagtaa ttagaagtat aaaagcacca acctatgacg
     3301 aagaaataac tgtgcaaatt aaagatgtac aagcgaaaaa cccaactcgt tcgttgaaag
     3361 actacttgat gaaaggcgaa atttgggaag taaaatagta taaaaacata gccataaaaa
     3421 atccccctaa atccatttct tagggggatt ttttatggct atgttactag tgtgatggta
     3481 ataagcaaat caacatacat gtatttacta tca
//
LOCUS       DAOSMY010000156         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26859_length_3507_cov_2.808227, whole genome shotgun sequence.
ACCESSION   DAOSMY010000156 DAOSMY010000000
VERSION     DAOSMY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26859_length_3507_cov_2.808227"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..429)
                     /locus_tag="PLD76_04390"
     CDS             complement(61..429)
                     /locus_tag="PLD76_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU27275.1"
                     /translation="MRYIYIFGTLFFTVYGQLILKWRLGNLSFLLPQGNISEKVVSLL
                     KLIFDPYIFSGFASAFIASLFWMAAMTKFQITEAYPFMSLAPAIVFLLGIWLLGETFT
                     WGKVIGLVLIIIGVIITVKL"
     gene            complement(453..1766)
                     /locus_tag="PLD76_04395"
     CDS             complement(453..1766)
                     /locus_tag="PLD76_04395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOU27276.1"
                     /translation="MKIAIVGTGYVGLVSGACFAEIGTHVTCIDIDIPKIENLKKGII
                     PIYEPGLEDIVARNYKAERLHFSTDIKDVLTNVEIVFCAVGTPPQHDGSADLQYVYQV
                     AETIGKYINNYVLLVNKSTVPVGTGKKVREIIQAELDKRGVKIDFDVASNPEFLKEGA
                     AIKDFMSPDRVVIGVESERAKEVMTKLYRPFLLNNFRVIFMDIPSAEMTKYAANAMLA
                     TRISFMNDVANLCELVGADVNMVRKGIGSDARIGNKFLYPGCGYGGSCFPKDVKALVN
                     TANEYGYPMRILQAVEDVNFQQKHILFEKLKKHFGNKFADKTVAIWGLTFKPNTDDMR
                     DAPSLVLIDELLQVGCKVKAFDPIITKLTDKAYKDVCIGKDIYDTANDADALVLVTEW
                     QEFRLPAWQVIHKTMKNHIVIDGRNIFDKTEMSQYGFIYEGIGIK"
     gene            complement(1798..2154)
                     /locus_tag="PLD76_04400"
     CDS             complement(1798..2154)
                     /locus_tag="PLD76_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU27277.1"
                     /translation="MGSLVGEKSYAFALRTIKAYQYIQTINEFVLSKQLLRSGTAIGA
                     LIREAEHAQSKADFLNKMNVALKEANETIYWITLLKDANYIDEKVYNSIQAEATEILK
                     LLISIVKTTKQTLNKK"
     gene            complement(2195..3490)
                     /locus_tag="PLD76_04405"
     CDS             complement(2195..3490)
                     /locus_tag="PLD76_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU27278.1"
                     /translation="MNVQDKKIAVIGLGYVGLPLAIEFGKKYRVLGFDIKAARIEELQ
                     AGEDSTLEADLEGMKIATDLYKKNEQIGLSFSSIQEDLKNFNTFIVTVPTPIDEFNNP
                     VLTPLISASRMIGNVLKKGDLVIYESTVYPGCTEEDCVPVLEKQSGLKFNKDFFCGYS
                     PERINPGDKVNTLTKIRKITSGSTPAIAEVVDNLYSSIITAGTYKAANMKVAEAAKAI
                     ENAQRDMNISFMNELALIFDRVGIDTNDVIEAAGTKWNFLKYRPGLVGGHCISVDPYY
                     LAHKAKALGYHPEVILSGRRVNNSISEFIASKTLKLMIQKGHTIKDSNVLILGITFKE
                     NCPDVRNTKVVDIRKELVEFGVNVDMFDPWAEAEEVHEEYGIDLVSKLDETKMYNAII
                     VAVAHDEFKAFDFAKYKKQGAIIYDVKAFVDRDLVDARL"
BASE COUNT         1080 a          715 c          490 g         1222 t
ORIGIN      
        1 ctattcctat gcaacaatca taaattaaaa gttgcattta caagttgaac tcaaggttta
       61 ttaaagtttt actgtaatta tcaccccaat aattatcaac actaatccaa ttacttttcc
      121 ccatgtaaat gtttcaccta acaaccatat tcctaataaa aaaacaatag caggagccaa
      181 gctcataaat ggataggctt ctgtaatctg aaacttagtc atcgctgcca tccaaaacaa
      241 ggaagcaata aacgcagatg caaaacctga aaaaatatat gggtcaaaaa taagttttag
      301 caaagatact actttttctg atatatttcc ctgtggcaat aaaaaactta aattacccaa
      361 tctccattta aggattaatt gtccataaac tgtaaaaaaa agggtgccga aaatatatat
      421 ataacgcata ttttttatga aatcatcaat tactacttta ttccaatccc ttcgtaaata
      481 aaaccatact gactcatttc agttttatca aatatatttc taccatcaat tactatatga
      541 tttttcatgg ttttatgtat cacttgccaa gcaggcaagc gaaattcttg ccattcagta
      601 accaatacaa gagcatcggc atcgttggct gtatcataaa tatcttttcc tatgcacaca
      661 tccttataag ccttatctgt taatttagta ataattgggt cgaacgcttt tactttgcag
      721 ccaacttgta acaactcatc aattaaaacc aacgaaggtg catctctcat atcatcagta
      781 ttgggtttaa atgttaaccc ccaaatagct actgtcttgt ctgcaaattt attgccaaag
      841 tgttttttta atttctcgaa caatatatgc ttttgttgga aatttacatc ttctacggct
      901 tgtaatatac gcataggata tccgtactca ttggctgtat ttacgagtgc tttcacatct
      961 tttgggaaac aagaaccacc gtaaccacaa ccaggataca gaaatttatt tcctatacgt
     1021 gcatccgaac caattccttt gcgcaccata tttacatcag ctcctaccaa ttcgcacaaa
     1081 ttagctacat cgttcataaa actgatgcgt gttgccaaca tggcatttgc tgcgtatttt
     1141 gtcatttcag ccgatgggat atccataaaa ataaccctaa aattattcag caaaaatgga
     1201 cggtacaatt tagtcatcac ttctttggca cgttcgcttt ctacacctat cactacccta
     1261 tcaggactca taaaatcctt gatagcagcc ccttccttta aaaattcagg attggacgca
     1321 acatcaaaat caatcttaac tcctcgttta tcaagttcag cttgtataat ttctcgtact
     1381 ttttttccag taccaacagg cactgtgctt ttgtttacca acaacacata gttgtttata
     1441 tatttaccta tggtttcagc tacttggtat acatattgca aatctgcact accatcatgt
     1501 tgaggaggtg tacctacagc acaaaaaaca atttccacat tagttaagac gtcttttata
     1561 tctgtactaa agtgtaaacg ttctgcctta taattacgag ctacaatatc ttctaaacct
     1621 ggttcgtaaa tagggataat tccttttttc agattttcaa ttttgggtat atcaatatca
     1681 atacatgtta catgggttcc aatttcagcg aaacatgccc ctgaaactaa acctacatat
     1741 ccggtaccta cgattgctat tttcatttac ttaattgata gttgagagtt gataattcta
     1801 ttttttgttt agggtttgtt tggttgtttt gactattgag attaacaatt ttaaaatttc
     1861 agttgcttct gcttgaatag agttatacac tttttcatct atatagttag catcttttaa
     1921 taaagtaatc caatatattg tttcatttgc ttctttaagt gcaacattca ttttgtttaa
     1981 aaaatcagct ttactttgtg catgctcagc ttctcttatc aatgctccaa ttgccgttcc
     2041 acttctcaat agttgcttgg atagtacaaa ttcgtttata gtttggatat attgatacgc
     2101 tttaatggtg cgtaaagcaa atgcatacga tttttcacca acaagacttc ccatatcctc
     2161 cactatcaac tgtcaactat ccactttcca cttattaaag tctcgcatct accaagtccc
     2221 tatccacaaa tgcttttaca tcatagatga ttgcaccttg tttcttgtat ttagcaaaat
     2281 caaatgcttt aaattcatcg tgagctacag ccacaataat ggcattgtac attttagttt
     2341 catctaattt gctcaccaaa tcaattccgt attcttcgtg tacttcttcg gcttcagccc
     2401 aagggtcaaa catatctaca ttcacaccaa attcaactag ttctttgcga atatctacta
     2461 ccttagtgtt tcgtacatct ggacaatttt ctttgaaagt tatgcctaaa attagaacat
     2521 ttgaatcttt aatggtatgc cctttctgaa tcattaattt cagtgttttg ctggctataa
     2581 attcagaaat actattattg actcttcgac ctgacaaaat cacctctgga tgatagccta
     2641 aagctttggc tttgtgtgct aaataatatg gatcaacgct aatacaatgt ccacccacca
     2701 aaccaggacg gtatttcaag aagttccatt ttgttccagc agcttcaatt acatcattgg
     2761 tgtcaattcc tactctgtca aaaattaatg ccaattcgtt cataaaggat atattcatgt
     2821 ctcgttgtgc attctcaata gctttagctg cttctgccac tttcatattg gctgctttgt
     2881 atgtgccagc tgttataatt gaactataca aattgtcgac aacctctgca atggcaggag
     2941 ttgaacccga tgttattttt ctaattttgg ttaatgtgtt tactttatca cccggattga
     3001 tacgttcggg cgaatagcca caaaagaaat ccttattgaa ttttaaacct gattgttttt
     3061 ctaacacagg cacacaatct tcttctgtac aaccaggata tacagttgat tcatagatca
     3121 ctaaatctcc cttttttaac acattaccaa tcatacgaga tgctgaaata aggggtgtta
     3181 acactggatt attaaattca tcaatgggag ttggtacggt tacaataaag gtgttaaaat
     3241 tttttaaatc ttcttgaata gatgaaaaag acaagccaat ttgttcattt tttttataca
     3301 aatcagtggc tattttcatt ccctccaaat cagcttccaa agtagaatct tctccagctt
     3361 gtaactcttc aatccgtgct gctttaatat caaatccaag aacacggtat tttttaccaa
     3421 attcaattgc taggggaagt ccaacgtacc ctaagcctat tactgcaatt tttttgtctt
     3481 gtacattcat gttaataatg attagtg
//
LOCUS       DAOSMY010000157         3494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_26986_length_3494_cov_3.247165, whole genome shotgun sequence.
ACCESSION   DAOSMY010000157 DAOSMY010000000
VERSION     DAOSMY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26986_length_3494_cov_3.247165"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2627)
                     /locus_tag="PLD76_04410"
     CDS             complement(30..2627)
                     /locus_tag="PLD76_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOU27279.1"
                     /translation="MNKKNYLYIISKQMCGIVCFLFLPLLMIAQGNNTTIRIDYESPK
                     EYEIADISVSGHHHYDTQTLIGLSGLAVGDKITLPGDATANVIKRFWKQGLFSSVRLE
                     ITSVFDNKIWLNLHIDQLPKISEINIHGVKKTEKEDILKNSAMSVGNELSANNIDKTT
                     NYIRKYFIEKGYYLVDVSVYSRDNVNQLNTSIVDIYVNKHAKIKVDKLLIEGNNTLST
                     KKIDAVMKKTNDKNNIFNFFRSKKFIPAEYEKDKISLIEKYNEIGYRDARIVWDSVYV
                     NDDKKTLTVHIRVDEGQKYYFGTISWLGNSVYTTDLLSRTLSIKSGDIFNQKLLNQRI
                     YDDEDGINNLYLNNGYLFFNLQLIETSIKDSLINFEIRINEGKQARINRVNIVGSDIL
                     YENVVRRELRTKPGDLFSKADIMRSMREIGSTGHFATEEGKMDIQVNPNMSDGTVDLT
                     YVLESKRNDQIELSAGYGQTSVVGILSLKFTNFSIQNMFDFSKYKPLPQGDGQTLTLR
                     AQTNGSYYQAYSISFLEPWLGGKRPNSLSLSAYYSIQSGMSSNYSSYYNSSSYYYNST
                     YDENSQNYWYASSYDPDKYIKTLGVSAGLGTRLNWPDDYFSIYGQASYQNYNLSNWDY
                     FAFSTGRANNLNFTVTLNRNSLDQPIYTRNGSDFSISASSTLPYSLFDNKDYSTMSDT
                     EKYKWIEYYKIKFNAKMFTPLSNDAKLVLFSRAEYGFLGYFNKDKRSPFETFYVGGDG
                     LTGYSSTYATETVALRGYKNGSLTPGGQAGNIYTKLSLELRYPLLLEETSVIWALGFV
                     EAGNAWKDFHNFNPFNLKRAAGVGIRLFMPMLGLLGLDWAYGFDKDNAVSSTGISGSQ
                     LHFVIGREF"
     gene            complement(2631..3371)
                     /locus_tag="PLD76_04415"
     CDS             complement(2631..3371)
                     /locus_tag="PLD76_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOU27280.1"
                     /translation="MNSYLNNIDTTKVPTHVAIIMDGNGRWAKQIGKNRLFGHQNAIT
                     AIRQSTEFAAEIGIKFLTLYAFSTENWCRPDDEVKGLMELLVATIEAETPTLNKNNVR
                     LLSIGDTSRLPESARLRLNHCIETTSKNTGLSLVLALSYSSRWEILDATKHIAEKVKQ
                     NTLSVNDITEELFSSYLTTRTIPDPDLLIRTSGEFRISNFLLWQLSYAELYFTPILWP
                     DFTKEEFQKAIIDYQKRERRFGKTSEQV"
BASE COUNT         1057 a          621 c          559 g         1257 t
ORIGIN      
        1 ctaatgttta atttaataag atatattaac taaaattctc gtccaattac aaagtgcaat
       61 tgactaccac ttatgcctgt actactcact gcattgtctt tatcaaaacc atatgcccaa
      121 tcaaggccta ataaaccaag cataggcata aataatcgaa tacctacccc cgcagctctt
      181 ttcaagttga aaggattaaa attatgaaaa tctttccatg catttcctgc ctctacaaaa
      241 cctaacgccc aaataacgga ggtttcttca agtaacaatg gatatcgcaa ctcaagagac
      301 agctttgtat atatatttcc tgcttgtcca ccaggggtta aggagccatt tttataccct
      361 ctaagtgcaa ctgtttctgt tgcatatgta ctactatatc ctgtcaaccc atcacctcct
      421 acataaaaag tttcaaaagg agaacgtttg tctttattga aataccctaa aaagccatac
      481 tctgctctac taaataatac cagtttagca tcattggata gaggggtaaa cattttggca
      541 ttaaacttta ttttgtaata ctcaatccac ttgtattttt cggtgtcaga cattgtacta
      601 taatctttgt tgtcaaagag agaataaggc agtgttgaac tagctgaaat ggagaagtct
      661 gatccattgc gcgtgtagat tggttggtct aatgagttac gattaagtgt tactgtaaag
      721 ttaaggttat tagcccttcc agtagaaaat gcaaaataat cccaattact taaattataa
      781 ttttgatacg aggcttgtcc atatatagaa aaataatcat cgggccaatt aagtcgagta
      841 cctaagcctg ctgaaacgcc aagagttttg atgtatttgt ctggatcata gctagatgca
      901 taccaatagt tttggctatt ctcatcatat gtactattgt agtaataaga tgatgagtta
      961 taatacgaac tatagttgct actcatgccc gattgaatag aataataggc tgataatgac
     1021 aaggagtttg gtctttttcc acctagccat ggttcaagaa aagatatact atatgcttga
     1081 taataggacc catttgtttg cgctctcagt gttaacgttt gtccatctcc ctgtggaagt
     1141 ggtttgtatt ttgaaaagtc aaacatgttt tgaatagaaa agttagtaaa ttttaagctt
     1201 aaaataccta ctacacttgt ttgtccgtat cctgctgata attctatttg gtcatttcgt
     1261 tttgattcta aaacgtatgt taagtcaaca gttccatcgc tcatatttgg attaacttga
     1321 atatccattt taccttcctc tgtggcaaaa tgtcctgtac tgccaatttc tcgcatggaa
     1381 cgcataatgt ctgctttgct aaacaaatct ccaggtttgg ttcttagctc acgcctcaca
     1441 acattttcgt ataatatatc acttcccaca atatttactc tattgatgcg tgcttgtttg
     1501 ccttcgttaa tgcgtatttc aaaattgata agactgtctt tgatgcttgt ttcaattagt
     1561 tgaagattga aaaataggta gccattattc aagtataggt tattaatacc atcttcatca
     1621 tcgtaaatgc gttgattgag tagtttttga ttaaaaatat ccccactttt aatagacaat
     1681 gttcttgata gcagatccgt ggtatataca gagttcccta accatgaaat agtaccaaaa
     1741 taatattttt gaccttcatc aacacgaata tgtaccgtaa gtgttttttt atcatcattt
     1801 acatagacag aatcccatac gatacgggca tctctatatc ctatctcgtt gtatttttca
     1861 ataagagata ttttatcttt ttcatattct gcaggaataa attttttact tctgaagaaa
     1921 ttgaaaatat tgtttttatc attggtcttt ttcatcacag catctatttt tttagtgctc
     1981 aatgtgttat tcccttcaat aagtaattta tccactttta ttttagcatg tttgttgaca
     2041 taaatatcta caatagaagt gttgagttga ttaacgttat ctctactata cactgaaaca
     2101 tctaccaaat aatagccttt ttcaataaaa tatttccgaa tataattggt cgttttatct
     2161 atattatttg cacttaactc attacctacc gacatggcac tattctttaa aatatcttct
     2221 ttctctgtct tttttactcc atgtatattt atttcagata ttttaggtaa ttgatcaata
     2281 tgtagattaa gccatatttt attatcaaat acagaggtta tttctagtcg tacagaagag
     2341 aatagacctt gtttccaaaa acgtttaata acattggctg tggcgtctcc tggtagtgtg
     2401 attttatctc ctaccgctag tcctgagaga ccaatcagcg tttgtgtatc ataatggtga
     2461 tgaccagata cagagatgtc tgctatttca tattctttag gactttcata atcaatacgg
     2521 atggttgtat tgttaccttg tgcaatcatc aaaagaggta gaaacaaaaa gcatacaatg
     2581 ccacacattt gtttcgaaat aatatacaaa taattttttt tgttcatgct ttacacttgt
     2641 tcacttgttt taccaaacct acgttctctt ttttgataat caattattgc tttttgaaat
     2701 tcttcttttg tgaaatctgg ccacaaaata ggcgtaaaat acaattcggc ataggataat
     2761 tgccataata agaaattact gatgcggaat tcgccacttg tcctaattaa taaatcggga
     2821 tcggggattg tgcgagttgt taaatatgac gaaaatagtt cttcggtaat atcatttacc
     2881 gacaatgtat tttgtttcac tttttcagcg atgtgttttg ttgcatctag aatttcccat
     2941 cttgatgagt aacttaatgc caacactaat gataatccgg tatttttaga tgtagtttct
     3001 atgcaatggt ttaaacgtaa tcgagcactt tctggcaagc gactcgtgtc tccaatactt
     3061 agtagacgta cattgttttt gtttagtgtg ggtgtttctg cttcaatggt tgcaaccaaa
     3121 agttccatta agccttttac ttcatcatct ggtctgcacc agttttctgt agagaaagca
     3181 tataaagtca aaaatttaat tccaatttca gcggcaaatt cggtgctttg ccgtatagct
     3241 gtaatcgcgt tttgatgacc aaataacctg tttttcccta tttgtttagc ccaccttcca
     3301 ttgccatcca taataatggc tacatgcgta ggaactttgg tagtatcaat gttgtttaaa
     3361 tagctgttca tatctattat cggcaaaatc tgcgtgtgtt aaataagtta tacgataagc
     3421 taatactagc aagagaatac caatcagtgt caaaaaatga tgtatttttt gtttgatatg
     3481 gatttgataa aatc
//
LOCUS       DAOSMY010000158         3491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_27017_length_3491_cov_2.967695, whole genome shotgun sequence.
ACCESSION   DAOSMY010000158 DAOSMY010000000
VERSION     DAOSMY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27017_length_3491_cov_2.967695"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLD76_04420"
     CDS             complement(<1..840)
                     /locus_tag="PLD76_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU27281.1"
                     /translation="MSNNKKKIINDPVFGFINIPGTLLYNLLQHPYVQRLTRIKQLGL
                     SSYVYPGAQHTRFQHSLGAMYLMTEAIKTLKSKGISISQHETEAACAAILLHDVGHGP
                     FSHALEGMIIPHLSHEEITLRIMQKINKEYEGKLDLALEIFQNTYHKTFLHQLVSGQL
                     DMDRLDYLRRDSFFTGVTEGNIGSARIIKMLNIKNDTLVVEAKGIYSIENFLMNRRLM
                     YWQVYFHKTAFAIEKMLIKTIERAKIIAKQDSTLFGTPSLLFFLRQEDNLTNDSALDL
                     FLNL"
     gene            complement(845..1681)
                     /gene="pyrF"
                     /locus_tag="PLD76_04425"
     CDS             complement(845..1681)
                     /gene="pyrF"
                     /locus_tag="PLD76_04425"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU27282.1"
                     /translation="MNRQELVENIQRKKSFLCVGLDTDITKIPEVLFDSCDDPMFVFN
                     KRIIDATADLCVAYKPNLAFYESLGLEGWDVLERTIDYIRTNYPDQFIIADAKRGDIG
                     NTSAMYARTFFGTMDFDAVTVAPYMVEDSVTPFLTYENKWVILLALTSNKGAFDFQFL
                     QDKETNERLFEKILHTSAQWGNTDNMMYVVGATKAEMLQDIRAIIPNHFLLVPGVGAQ
                     GGSLQEVAKYGMNAECGLLVNSSRQILYASSGEDFAEAARLEALAVKQEMEQLLKDAH
                     LV"
     gene            complement(1699..2778)
                     /gene="prfA"
                     /locus_tag="PLD76_04430"
     CDS             complement(1699..2778)
                     /gene="prfA"
                     /locus_tag="PLD76_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU27283.1"
                     /translation="MSTLLQKLEGLATRFEEVSTLITDPSVIADMKRFVKLNKEYREL
                     EKINGARIKYEQLIANIAEAKEILDKENDEELREMAKMELEQSSPLVATMEEEIKLLL
                     VPADPEDSKNAIVEIRGGTGGDEAAIFAGDLYKMYIRYCETKKWKVEVTNISEGTAGG
                     YKEVIFTVSGENVYGTLKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEAEEFDIEL
                     KESDVRVDTFCSSGAGGQSVNTTYSAIRLVHIPTGITVQCQDEKSQHKNKAKAMIELR
                     SRIYAIEHQKYLDEIGSKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLSAFM
                     DGDIQDCIDQLIIAENAERMKENEL"
     gene            complement(2797..>3120)
                     /locus_tag="PLD76_04435"
     CDS             complement(2797..>3120)
                     /locus_tag="PLD76_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU27284.1"
                     /translation="HGMVHCSGGAQTKILHFIDNLHVIKDNLFPVPPLFKTIQEESGT
                     DWAEMYKVFNMGHRLEVYLPKEYAQQVIDIAQSFGIDAQVIGRVETAEKKKLTINSEF
                     GTFVY"
     assembly_gap    3121..3130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3131..>3491)
                     /locus_tag="PLD76_04440"
     CDS             complement(<3131..>3491)
                     /locus_tag="PLD76_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOU27285.1"
                     /translation="VDSTVTCRMKRADVIDNACIEGGDVIVGLASYGQATYEKSYNGG
                     MGSNGLTSARHDVFANYLANKYPESYDASMPSELAYSGSLQLTDAVPNSPIDAGKLVL
                     SPTRTYAPVVKQLLDTMR"
BASE COUNT          942 a          704 c          652 g         1183 t
ORIGIN      
        1 gaggtttaaa aataaatcta acgcactatc gttggtcaaa ttatcctctt gacgtaaaaa
       61 aaagagcaac gatggggtac cgaatagcgt gctgtcttgc ttggcaataa ttttcgctct
      121 ttcgatggtt ttgataagca ttttttcgat tgcgaaagct gttttatgaa aatatacttg
      181 ccaatacatc aggcgtctgt tcatcagaaa attttcgata gaataaattc ctttggcttc
      241 tactactagc gtatcgtttt ttatgtttaa cattttgata atacgtgccg aaccaatatt
      301 gccttcggta actcccgtaa aaaaactatc tctacgcaaa taatcgagcc tatccatatc
      361 caattgaccc gaaactaact gatgcaaaaa tgttttgtga taggtgtttt gaaagatttc
      421 taaggctaaa tcaagtttcc cttcgtattc tttattgatt ttttgcatga tacgcaaggt
      481 tatttcttcg tgacttaggt ggggaatgat cataccttct aaggcgtgag aaaacggacc
      541 atgccccaca tcatgcaata atatggcggc acaagcagct tctgtttcgt gttgcgaaat
      601 gctaattcct tttgatttga gtgttttaat tgcttctgtc atcaagtaca tagcccccaa
      661 ggagtgttga aagcgtgtgt gctgtgcccc aggatataca tacgatgata aaccaagttg
      721 tttgatgcgt gtaagtcgtt gcacataagg atgttgcaat aaattataca acaatgtgcc
      781 tggtatgttg ataaaaccaa aaacagggtc gttaattatt ttctttttgt tgttagacac
      841 ggcattacac taaatgagca tctttcaata attgttccat ttcttgtttc acagcaaggg
      901 cttcaaggcg agcggcttct gcaaaatctt ctcccgaaga ggcatatagt atttgtcgcg
      961 acgaatttac taacagacca cattcagcat tcataccgta ttttgccact tcttgcaaac
     1021 tgccaccttg tgctccaaca cctggaacaa gcaaaaaatg attcggaata atagcacgaa
     1081 tatcctgtaa catttcggct tttgtagcac ctactacata catcatatta tcggtatttc
     1141 cccattgagc agatgtgtgg agtatttttt cgaacaagcg ttcgttggtt tctttgtctt
     1201 gtaaaaattg aaaatcaaaa gctcctttgt tcgatgttaa cgccaacaaa atgacccact
     1261 tattttcgta tgttaaaaat ggtgttacgc tgtcttcgac catataagga gctactgtaa
     1321 ctgcatcaaa atccattgtt ccaaagaacg tacgtgcata catagccgag gtgttgccta
     1381 tatcgccccg tttagcatca gcaataataa attggtcagg gtaatttgta cgaatataat
     1441 caatggtacg ctctagcaca tcccaacctt ctaatcctaa actttcgtaa aaagctaagt
     1501 tgggtttgta tgcaacgcat aaatcagccg tagcatcaat aattcgtttg ttaaacacaa
     1561 acatggggtc gtcgcaactg tcaaaaagta cttctggtat tttagtaata tcggtatcta
     1621 aaccaacaca tagaaacgat tttttgcgtt gtatattttc aactaattct tgtctgttca
     1681 tagtatcaaa gtgtatcgtt ataattcatt ttctttcatt ctttctgcat tttcggcaat
     1741 tatcaactgg tcgatacagt cttgtatgtc gccatccata aaagcagata aattatagat
     1801 agtataatta atgcggtggt cggtaatacg tccttgcggg taattgtagg tgcgaatttt
     1861 tgccgaacga tcgcctgttg aaaccattgt tttgcgtttc gacccaattt cgtcgaggta
     1921 tttttggtgt tcaatggcgt aaattctgga acgaagctca atcatcgctt tggctttgtt
     1981 tttgtgttgc gatttttcat cttggcattg tacggtaata cctgttggta tatgcaccaa
     2041 acggatagcc gaataggtag tgtttaccga ctgccctcca gctcccgacg aacagaatgt
     2101 atcaacacgt acatcgcttt cttttagttc tatatcaaat tcttctgctt cgggcaatac
     2161 agccaccgtt gcagctgagg tatgcacacg tccttgtgtt tctgtttgtg gtacacgttg
     2221 tacacggtgt acgcctgatt cgtattttaa ggtaccatac acattttcgc ctgatacagt
     2281 aaagattact tctttgtagc cacctgcagt accttcgcta atgttcgtaa cttctacttt
     2341 ccattttttt gtttcgcaat agcgtatgta cattttatac aaatctccag caaaaatagc
     2401 tgcttcatcg cctcctgttc cgcctctaat ttctacaatg gcatttttac tgtcttctgg
     2461 gtcggctggc acgagcaata atttgatttc ttcttccatt gtagcaacga gtggagaaga
     2521 ttgttctaat tccatttttg ccatttctcg tagttcctcg tcgttttctt tgtcaagaat
     2581 ttctttggct tcagcaatat tggctattaa ttgttcgtat ttaatgcgag ctccgttgat
     2641 tttttcaagt tcgcggtatt ctttgttcag tttaacaaaa cgtttcatat cagctatcac
     2701 agaagggtct gtaatcaatg tagaaacttc ttcaaaacga gtagctaaac cttctaattt
     2761 ttgcaataat gtgctcatat acttttttcg ggcttgttaa tatacaaaag tgccgaattc
     2821 actgttgata gttaattttt tcttttcggc tgtttccact cttcctataa cttgtgcatc
     2881 tatgccaaac gattgtgcta tatcgattac ttgctgagcg tattcttttg gcaaatatac
     2941 ttctagtcgg tgtcccatat taaatacttt gtacatttct gcccaatccg taccactttc
     3001 ttcttgaatg gttttaaata agggaggtac tggaaataag ttatctttaa taacgtgtag
     3061 attgtctata aagtgcaata tttttgtttg agcacctcct gagcaatgca ccatgccatg
     3121 nnnnnnnnnn acgcattgtg tctaacaatt gctttactac tggagcatat gtgcgagtag
     3181 gagagagtac taatttacca gcatctattg gcgaattggg aacggcatct gtgagttgta
     3241 acgaccctga atatgccaat tcagagggca tacttgcatc gtagctttcg ggatacttat
     3301 tggctaaata gttagcaaat acatcatggc gagccgaagt tagtccattg ctacccatac
     3361 caccgttgta ggatttttcg tacgttgctt gaccgtaaga ggcaagtccc acaattacat
     3421 cgcctccctc aatgcaagca ttgtcaatta catcagcacg tttcatgcgg caagtaactg
     3481 tactatctac g
//
LOCUS       DAOSMY010000159         3475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_27177_length_3475_cov_3.286257, whole genome shotgun sequence.
ACCESSION   DAOSMY010000159 DAOSMY010000000
VERSION     DAOSMY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27177_length_3475_cov_3.286257"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1896
                     /locus_tag="PLD76_04445"
     CDS             163..1896
                     /locus_tag="PLD76_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOU27286.1"
                     /translation="MTKKTETVRLITFFKEIFYPKLFSSLRLYSKEKFLSDTMAGVIV
                     GIVAIPLAIAFGIASGVGPTEGLITAVIAGFIISFFGGSKVQIGGPTGAFIVIIYGII
                     QQHGLTGLLVATIMAGVMLVLMGVFKLGSIIKFVPYPVIVGFTGGIALTIFSTQINDF
                     FGLGISNLPADFIHKWISYFSHFTAIHWWSFIIALISLFIILVMPLFSKKIPGSLVAI
                     ILATILVYFLKLTSFAGVDGIKTIQDIYILPEGIPMPHLPSIALAENQTLWGLIQTLF
                     PAAFTIAMLGAIESLLSAMVADGVIRDKHNSNTELIAQGMANIVVPFFGGIPATGAIA
                     RTMTNINNGAKTPIAGIIHAIVLLLVLLFLGKFVGYIPMACLAAVLIIVAYHMSGWRS
                     FISLSKNPKSDFVVLMTTFLLTVIFDLTIAIEIGLLLAVVLFLKRTNEATIIRVFQHK
                     IDPAENSDILLQDDPLYIPKEVDVYEIDGPYFFGIATKFDEVMETVGEKPKVRIIRMR
                     KVSFIDSTGIHHLQNLCEQSHREKIVIILSGVNKHVRGALMKANFFSILPEERVCSTI
                     HEALELSEQYI"
     gene            2094..>3475
                     /locus_tag="PLD76_04450"
     CDS             2094..>3475
                     /locus_tag="PLD76_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU27287.1"
                     /translation="MKKTKSLLLYVFLSLFGIFSQAYAQVPLASHDVMLQGFMWNSHT
                     STKWSVLKPQASEIAESFTLVWLPPSAVAEGGGASNVGYHPYQWSNQNSSWGTRTELE
                     ALITDLKAGGAKVLADIVVNHRAGNGWCDGFTTDNFGTYGVFTLKASHITKDDEASGQ
                     SACSGKLSDHNDWNFDKPIEYWGGYQAARDLAHSLTEVRDAVKAYLKWMKNVIGYDGF
                     RWDVVKGFNPAYIKEYIQAAGAYLSVGEYYSPNYDDLKGWVEATNKTALVFDFGFKKA
                     IFQWGGGSDYSKLGWMDGSILRPAGLVHNPGMRQYAVTFVDNHDTSSPHPSGYGWEYK
                     GDKAKANAIMLAAPGIPCVFWRDWVAVKADIKKMIAARKKVGLNSNSDVRVTNTNGYY
                     ESHGVGTTGELICRVGSWNGTPSGFTLECSGTGWAYYTKGGSTAPTITVSPQGGYVGV
                     NGKVTITASTG"
BASE COUNT         1042 a          485 c          713 g         1235 t
ORIGIN      
        1 ccaatagcgt cgatggtact gcacttgtgt gggagagtag atagccgccg ttttttacca
       61 aaggaggaga tgtttttata cgtctcctcc tttgtcgttt tagaatttat taggtctatt
      121 ctttactcta taatttgtct gattttaacg ttttatacat ttatgactaa aaaaactgaa
      181 actgtaagat taataacttt ttttaaagaa atcttttatc caaaattatt ttcttcttta
      241 cgtctttatt ctaaggaaaa gtttttgtct gacactatgg ctggtgtaat agtgggtatt
      301 gttgctatac cactggctat tgcatttggt attgcatcag gagtcggtcc tactgaggga
      361 cttattactg cagttattgc tgggtttatt atatcatttt ttggaggttc aaaagtgcag
      421 attggaggtc caacgggagc atttattgta attatttatg gtataattca acaacatggt
      481 cttacgggat tattggtagc taccattatg gctggtgtaa tgttggtatt gatgggggtt
      541 tttaaattag gttctattat taagtttgta ccttatcctg ttattgttgg ttttactggt
      601 ggtattgcac ttacaatttt ttctacccag attaatgatt tttttggttt aggaatatct
      661 aatttacctg cagattttat tcataaatgg atttcttatt tttcccattt tacagcaata
      721 cattggtggt cttttataat tgcattaatt agtttgttca ttattcttgt tatgccttta
      781 ttttcaaaaa agatacctgg ttctttggtt gcaatcatat tagctactat ccttgtttat
      841 ttcttaaaat taacatcatt tgcaggagta gacggaatta aaacaatcca agatatctat
      901 attttaccag aaggtattcc aatgcctcat ttgccatcaa ttgcattagc agagaatcaa
      961 acgttatggg gattgataca aacgttattc ccagcagctt ttacgatagc tatgttaggg
     1021 gctattgaat cgttactttc tgcaatggtt gcagatggag ttatacgtga taaacataat
     1081 tctaatacgg agttgattgc acaagggatg gcgaatattg tagttccttt ttttggaggt
     1141 atacctgcta caggagctat tgctagaacg atgactaaca tcaataatgg tgctaaaacc
     1201 cctattgcag gaattataca tgctattgtt ttattgttag ttcttttatt tttaggaaaa
     1261 tttgttggat atattccaat ggcatgttta gctgctgtct taattattgt ggcatatcat
     1321 atgagtggat ggagatcgtt tatatcttta tcaaaaaatc caaaatcaga ttttgtggtt
     1381 ttaatgacaa cttttttact aacggtaatt tttgatttaa ctatagcgat tgaaatagga
     1441 ttattattag cagtagtatt attccttaaa agaaccaacg aagcgactat aatacgagtt
     1501 tttcaacata aaattgatcc tgctgagaat tctgatattc ttcttcaaga cgacccatta
     1561 tatattccca aagaggttga tgtttatgaa attgatggac cttatttttt tggtatagct
     1621 accaaatttg atgaagtaat ggaaacagtg ggagaaaaac ccaaagttcg tataattcgt
     1681 atgcgtaaag tttcttttat tgattctacg ggcattcatc atttacaaaa tttatgtgaa
     1741 caatcgcata gggaaaaaat agtaatcatt ttgtctggtg tgaataaaca tgtacgagga
     1801 gcattaatga aagccaattt ttttagtata ttaccagaag aaagagtttg ttcaacaatt
     1861 catgaagctt tagaattatc tgaacaatac atttgataga tgttttataa ggtagacaga
     1921 tatataaagt ctcacaacgt tatcatgtag aaaaagaaaa ttacatagaa gtgctaatat
     1981 tgtttattac aagaatgtta aatcttacca aaatgtggag aaggtatggg ataattcatt
     2041 tttttagcta aatttgtact ctaaaagtta attaaacctt aatattttat acaatgaaaa
     2101 aaacaaaatc acttttactg tatgtctttt tgtctctttt tggcatattt tctcaggcat
     2161 acgctcaggt tcctttggct tctcatgatg ttatgcttca gggatttatg tggaattcac
     2221 atacgtctac taaatggagt gttctaaaac ctcaagcgtc tgaaatagct gagtcgttta
     2281 ctttagtatg gttaccaccg tcagcggttg ccgaaggtgg cggagcgtct aatgtaggat
     2341 atcaccctta ccaatggtct aatcagaata gttcgtgggg tacacgtact gagttagaag
     2401 ccttaattac agatttaaaa gcaggtggag ctaaagtatt agctgatatt gtggtaaatc
     2461 atcgtgcagg taatggttgg tgtgatggtt ttacaacaga taattttggt acctatggtg
     2521 tttttacttt aaaagcttca catattacga aagatgacga agcttcagga caatcagcat
     2581 gttctggtaa gttaagtgat cataatgatt ggaattttga taaaccaata gaatattggg
     2641 gaggatatca ggcggctcgc gatttagctc atagtcttac cgaagtgaga gatgccgtaa
     2701 aagcatattt aaaatggatg aaaaatgtaa ttgggtatga tggttttcgt tgggatgtgg
     2761 taaagggttt caatcctgct tatattaaag aatatataca agcagcagga gcttatttgt
     2821 ctgtaggtga gtattatagt cctaattatg atgatttaaa aggttgggta gaagcaacta
     2881 ataaaacggc cttagtgttt gactttggat ttaagaaggc tatttttcaa tggggtggag
     2941 gctctgatta cagtaaatta ggttggatgg atgggagtat cttacgtcct gcgggattgg
     3001 tacataatcc tggtatgcgt caatatgctg ttacatttgt agataaccat gatacgtcat
     3061 ctcctcatcc atcaggttat ggatgggaat ataaaggaga taaagccaaa gccaatgcta
     3121 ttatgttagc agcacctggg attccttgtg tattttggag agattgggta gccgtaaaag
     3181 ctgatatcaa aaaaatgatt gccgctagaa aaaaagtcgg tttaaatagt aactctgatg
     3241 tgcgagtgac taatactaat ggatattacg aatcgcatgg agtaggtact actggcgaat
     3301 tgatttgtcg tgttggtagt tggaatggta ctccaagtgg atttactctt gagtgtagtg
     3361 gaacaggttg ggcttattat accaaaggag gaagtactgc tcctactatt acagtttctc
     3421 ctcaaggagg ttatgtagga gtaaacggaa aagttactat aactgcttct actgg
//
LOCUS       DAOSMY010000160         3424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_27710_length_3424_cov_3.518551, whole genome shotgun sequence.
ACCESSION   DAOSMY010000160 DAOSMY010000000
VERSION     DAOSMY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27710_length_3424_cov_3.518551"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1547)
                     /gene="ilvB"
                     /locus_tag="PLD76_04455"
                     /pseudo
     CDS             complement(72..1547)
                     /gene="ilvB"
                     /locus_tag="PLD76_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
     gene            complement(1629..>3424)
                     /gene="ilvD"
                     /locus_tag="PLD76_04460"
     CDS             complement(1629..>3424)
                     /gene="ilvD"
                     /locus_tag="PLD76_04460"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOU27288.1"
                     /translation="GRKMAGARALWRANGMQENQIGKPIIAVVNSFTQFVPGHVHLHQ
                     IGQLVKQEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIADSVEYMVNAHKA
                     DAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGELDNKHLDLIDAMIQSADPS
                     VSDEEVDRIERAACPTCGSCSGMFTANSMNCLNEAIGLALPGNGTIVATHANRKQLFI
                     EAAKMIVDNTYKYYANGDDSVLPRSIATRDAFLNAMVLDIAMGGSTNTILHTLAIAYE
                     GNVSFNMDDIDALSRKTPCLCKVAPNSQKYHIQDVNRAGGILGILAELAKGNLINTSV
                     KRVDGLTLQEAIDRYDITSQSASELAIKKYKSAPAHRFNLVMGSQETYYPALDTDREN
                     GCIRSVDKAYTKDGGLAILKGNIAIDGCVIKTAGVDESIWKFSGTAKVYQSQDDACYA
                     ILKGDVKAGDVVVITHEGPKGGPGMQEMLYPTSYIKAKHLGKECALITDGRFSGGTSG
                     LSIGHISPEAAAGGAIGLVKDGDIIDIDIPNRSINVRISDNELENRRKEEEGKGKEAW
                     KPIRDRKISTALKAYASMVTSADKGAVRVVE"
BASE COUNT          969 a          721 c          568 g         1166 t
ORIGIN      
        1 tcttcttttc cattaatatt gtatgctttg gctatcgtta taaagtctgg attttccatt
       61 ggtgttgatt aatagcgttc ttggtgaaaa agttcttgcc attgtcgtac cattcctaaa
      121 aagtgattgt ttaaaataat catttttacg ccaatttttt cttgcataat agttcctaac
      181 tcttgtattg tcatttggaa acctccatcg ccacagaata aacatacggt acggtcaggg
      241 gcacctattt ttgcaccaat tgctgcgggg ataccgaagc ccattgttcc taatcctcct
      301 gaggtaacat tgctccgttt ttgtgtaaaa cggaagtaac gatgtgccat catttggttt
      361 tgacctacgt cacttactaa aatagcacta tggttggtag ctttagatac ttttaaaaca
      421 acctcaccca ttttgatagc accctcggta gattctgttt ctttttgaat gacaaaatcg
      481 tactcctttt gttcatgttc cttaaaagac gctatccatt ctgtatgaga atttttcaca
      541 agatattgtg ttactgcagg aagtgttgtt tttacatctc ctaaaacggc tacttctgtt
      601 tgtacgtttt tgttaatttc agcaggatca atatcaaagt gaataacttt agcctgttta
      661 gcatattcat ttaaattccc tgttacacgg tcgtcaaaac gcataccgat agcaattaat
      721 acatcacatt cgttggtttt aaggtttggt ccgatatttc cgtgcattcc cagcatacct
      781 ttgtttaaag gatggtcggt tggcattgct gattctccca ataatgtcca tgccgcagga
      841 atgtttgctt tttctataaa tgctagtaat tcatcttcag catttccaag cgtaacacct
      901 tgaccaacta aagcaaaagg tttttttgct tcattaatta attttgcagc ttgtttggct
      961 accgtatcat caagtttaca tgtaggaata taactacgaa taaatgtaca tttttgtgga
     1021 ataaaatcta cgttttccaa ttgtgcattt ttcgcaatat ccaatacaac aggtcctggt
     1081 cgtccacttt gtgcaatata aaatgctcgt gctactgccc atgctatttc atctgctttg
     1141 cgtatttgat aactccattt agtgatgggt tgggtaatac ttactacatc tatttcttga
     1201 aaagcatctg ttcctaataa agcggcactt acttgtcctg taattacaac taaaggggta
     1261 ctatctagca tagcatcact aatgcctgtt actgcattgg tggcagctgg accagatgtt
     1321 acaatacata ccccaacttt acccgatacg cgggcatagc cttgcgctgc atgtgtggct
     1381 ccttgttcgt ggcgagttaa gatataacgt agcctatcgt aataatcgta catagcgtca
     1441 aatactggaa taatggcacc tccagggtag ccaaagatag tatcaacacc ttcgtttaaa
     1501 agggattcca ataaggcgta tgaaccttgc attgtttttt tttccatatt atatagttta
     1561 tataaaatca ataaaattga aatagtataa ttgtaaacaa aaagaataaa agagtatctt
     1621 agtaaagatt actcaacgac tcgtacagcc cccttatcag cagatgttac catgcttgca
     1681 taggctttta gtgctgtaga aatttttctg tcacggatag gtttccaagc ttctttacct
     1741 ttaccttctt cttcttttcg tctattttct agttcattat ctgaaatacg tacattgata
     1801 cttctatttg gaatatctat atcaataata tcgccgtctt ttactaaacc aatagcacca
     1861 ccagctgcag cttcaggcga aatatgtcca atagataaac cagaagttcc tcccgagaaa
     1921 cgtccatctg taatcagcgc acattctttg cctaagtgtt tagccttgat ataggaggta
     1981 gggtatagca tttcttgcat tcctggacct cctttgggtc cttcgtgtgt aataactaca
     2041 acatcacctg cttttacatc tccttttaaa atagcataac atgcatcatc ttgcgactga
     2101 tatactttgg ctgtgcctga aaatttccaa atactttcat ctactcctgc tgttttaatt
     2161 acacagccgt caatagcaat gtttcctttc agaatagcta gtccaccatc ttttgtatag
     2221 gctttatcta cacttctgat acatccattt tcacgatcag tatctaaagc tggatagtat
     2281 gtttcttgcg aacccataac caaattaaaa cggtgagcag gtgcactttt atatttctta
     2341 atggctaatt cgcttgcact ttggcttgtt atatcatacc tgtcaatagc ttcttgcaat
     2401 gttaaaccat ctactctttt aacgctcgta ttaattaagt tcccttttgc caactctgcc
     2461 aaaataccta aaataccgcc agcacggtta acatcttgaa tgtggtattt ttgtgaatta
     2521 ggagcaactt tacacaagca gggtgtttta cgagataagg catcaatatc atccatattg
     2581 aaagaaacat tcccttcgta tgcaatggct aaagtatgta aaatagtgtt tgtagatcca
     2641 cccatagcta tatccagtac cattgcattt aaaaaagcat ctcgtgtagc tatagaacgt
     2701 ggtaaaacac tatcgtcccc atttgcgtaa tatttatacg tattatcaac aatcatttta
     2761 gctgcctcaa tgaataattg ttttcgatta gcatgtgtgg caacaatagt tccattacct
     2821 ggtaatgcta aaccaatagc ttcgtttaaa caattcatag agtttgcagt aaacattcct
     2881 gaacatgacc cacaggtagg gcaggctgcc cgttcaattc gatcaacttc ctcgtcagat
     2941 acacttggat ctgccgattg tatcatggca tcaatcaaat ctaaatgctt gttgtcaagt
     3001 tctccagctt ccattggacc cccagaaaca aaaattgtag gaatatttaa tcgcattgca
     3061 gccattaaca tacctggagt aattttgtcg caattgctta tacataccat cgcatctgct
     3121 ttgtgtgcat ttaccatgta ttcaacgcta tcagctatta aatcacgact tggaagcgaa
     3181 tataacatac catcatgtcc catagcaata ccatcatcaa tggctatggt attaaattca
     3241 gctgcaaaac agcctaattt ttcaatttct tgttttacca actgacctat ttggtgtaaa
     3301 tgaacatgac ctggtacaaa ttgagtaaac gaattgacaa ctgcaataat gggtttccca
     3361 atttgatttt cttgcatacc gttggcacgc cacaatgctc tggctcctgc catttttctg
     3421 cctt
//
LOCUS       DAOSMY010000161         3417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_27775_length_3417_cov_3.804283, whole genome shotgun sequence.
ACCESSION   DAOSMY010000161 DAOSMY010000000
VERSION     DAOSMY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27775_length_3417_cov_3.804283"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="hisD"
                     /locus_tag="PLD76_04465"
     CDS             <1..667
                     /gene="hisD"
                     /locus_tag="PLD76_04465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU27289.1"
                     /translation="PGNQYVTAAKQLVSLKDVAIDMPAGPSEVEVIADETANPAFVAA
                     DLLSQAEHGADSSAILLTTSNELATAVQQEIQQQLPLLSRAEIAVKALNNSKIILLHT
                     MDDIIEWTNEYAPEHLIISTKNYTDIANRITNAGSIFLGYLTPESAGDYASGTNHTLP
                     TNGYAKAYSGVNLDSFKKKITYQELTKEGIQAIGSTIEVMAENELLTAHKNAVTLRLQ
                     SLV"
     gene            764..1912
                     /locus_tag="PLD76_04470"
     CDS             764..1912
                     /locus_tag="PLD76_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU27290.1"
                     /translation="MNTPVEKPYTFDRVIRIIITLVVLFALFLLIKKLSTVLLPFLIA
                     LLIAYLLQPVVHFFQYTLKLKYRLLAVISTLILFFGTIVGLLWFIIPLASTEIQHISQ
                     TISLYVQGINMDTVLPETWQKEIRIFLSQLNIKSVLENESLMSVLKNSTPKLWLLINN
                     SFSFILGFSVIFIILLYIIFILKDYDKITTQWIQIIPVKYRHITAEIIADIEKGMNHY
                     FRGQFLIAFIVGILFTIGFSIIGLPLAIVFGIFIGILNLVPYLQILAVAPAMLLVFLQ
                     SIETNQPFGSCFLGLLIVFILVQSFQDLVLVPKIMGKVTGLNPAIILLSLSIWGALLG
                     IVGMILALPMTTLLISYYKRFVINKESLSDNIDATENEIEQEKTKESE"
     gene            1937..2251
                     /locus_tag="PLD76_04475"
     CDS             1937..2251
                     /locus_tag="PLD76_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOU27291.1"
                     /translation="MILSTIHTIPNREIEAILGMARGSTVRTRNIGNDIMASLKNIVG
                     GEIEEYTKLQAQSREQAIQRMVEDAKKMGADAIVGILITTSMVMQGASEILAYGTAVK
                     LK"
     gene            2393..3028
                     /gene="pdxH"
                     /locus_tag="PLD76_04480"
     CDS             2393..3028
                     /gene="pdxH"
                     /locus_tag="PLD76_04480"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOU27292.1"
                     /translation="MDNLQDLRKNYEFSYINENQLKDNPLEQFSIWMKEAINSAELEP
                     SAMVLSTVDAQGFPHSRMVLLKDFSESGFVFFSNYESDKAIQMKSNHAVSILFFWQHL
                     ERQVRIEGIVEKIAEIESDAYFLSRPIEHKLGTWASAQSKPIPSSTFLEEQFLYFKDK
                     FKHDIPRPPHWGGYSIKPSRFEFWQGRPNRLHDRFVYKLNESDWTIERLAP"
     gene            3052..>3417
                     /locus_tag="PLD76_04485"
     CDS             3052..>3417
                     /locus_tag="PLD76_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27293.1"
                     /translation="MHKYFVFILILGLLQNLSAQAASFQAKLDSVIYVKSQKTTYVYD
                     TDDRIEYMLLQGWVEHQNKWDYPTKTSYEYDNTNRLVLSFYSTWNLSSQNYQIQSKRS
                     YSYTEAGTIFQSIASVWSNN"
BASE COUNT         1160 a          543 c          569 g         1145 t
ORIGIN      
        1 tccaggaaat caatatgtaa cagccgctaa gcaacttgta agtttaaaag atgtagctat
       61 tgatatgccg gctggtccat cggaagtaga agtaattgct gacgaaactg ctaaccctgc
      121 gtttgttgct gcagatttgc tttcgcaagc tgagcatggg gcagacagtt ccgctatttt
      181 gctaacaact agcaatgaat tagcaactgc tgtgcaacaa gaaatacagc aacagcttcc
      241 tcttttgtcg cgtgctgaaa ttgccgttaa agcattgaat aatagcaaaa taattttgtt
      301 acatacaatg gatgatatca ttgaatggac aaacgagtac gctcctgaac atttaattat
      361 ctctacaaaa aattacacag atatagccaa tagaatcacc aatgcaggtt caatattttt
      421 gggttactta acacctgaaa gtgctggcga ttatgcttca ggcacaaacc acaccttacc
      481 tactaacgga tacgcaaagg catatagtgg tgtaaatcta gatagtttta agaaaaaaat
      541 tacgtatcaa gaattaacaa aagaaggcat acaagccata ggaagcacta ttgaagtaat
      601 ggctgaaaat gaattgttaa cagcacataa aaatgctgtt acgctacgct tacaatcact
      661 tgtataaata tatagttaat ttttgctatt tttattaaac ttttattcca ttttatttgt
      721 atgtttgtaa ttgaaatata acgatagtat aaacattctg aaaatgaata cacctgtgga
      781 aaaaccatac acattcgacc gcgttatccg cattattata acgttggtag ttttgtttgc
      841 cttatttcta ctaataaaaa aactaagtac agtactcctt ccctttttaa ttgccttact
      901 tattgcatat ttactacaac ctgttgttca tttttttcaa tacactctca aattgaaata
      961 cagacttttg gctgtcatca gtacactgat actttttttt ggcactattg taggtctttt
     1021 atggtttatc attccattgg caagtactga aattcaacac atttctcaaa cgatatcatt
     1081 atacgttcaa ggtataaata tggatacggt attgcctgaa acgtggcaaa aagaaattcg
     1141 catattttta agtcagctta atataaaatc tgtattagaa aacgagagtt tgatgtctgt
     1201 acttaaaaat tctaccccaa aactatggct attgattaat aactctttta gttttatact
     1261 aggattttca gtaatattta tcattttatt gtacattatt tttatcttaa aagattatga
     1321 taaaattaca acacagtgga ttcaaatcat acctgtaaaa tatcgtcata ttactgctga
     1381 aataattgca gacattgaaa aaggaatgaa tcattatttt cgaggtcaat ttttaatagc
     1441 cttcatagtt ggcattcttt tcacaatagg ttttagcatt attggtttgc ctttggctat
     1501 tgtttttggt atatttatag gaattttaaa tttagtaccc tatctgcaaa tacttgcagt
     1561 agcacctgct atgttactag tttttttaca atcaattgaa acgaatcaac cttttggttc
     1621 ctgtttttta gggttactga ttgtttttat tctagtgcaa agttttcaag atcttgtatt
     1681 ggttcctaaa ataatgggca aagtaacagg tttaaaccct gccattattt tattatctct
     1741 gtctatttgg ggcgcattac ttggcattgt tggtatgata ttggcattgc ctatgacaac
     1801 cttattgata tcttattaca aaagatttgt gattaataag gaatcattgt cagacaacat
     1861 tgatgctact gaaaatgaaa ttgaacaaga aaaaacaaaa gaatcagaat aactaacttt
     1921 ctaaaaactt attacgatga ttttatctac aatccacact attccaaacc gtgaaattga
     1981 agctattttg ggaatggcta gaggtagtac tgttcgtaca agaaatattg gcaacgacat
     2041 aatggcaagt ttaaaaaata ttgtgggcgg agaaattgag gaatacacca aacttcaagc
     2101 acaatcgcgt gaacaagcca tacaacggat ggttgaagat gctaaaaaga tgggagccga
     2161 tgcaattgtt ggtattctta ttaccacatc catggtaatg cagggagcat cagagatact
     2221 agcttatgga acggctgtaa aattaaaata atatgatgta tgtagtatta gggttacagg
     2281 cattatcagc tgtaattata ttcgttatta tagttttctt aattattcgt agaagaaaaa
     2341 ttaagaaaac agaaaccttt gaagatagaa gtaactaaaa aaacactcat atatggataa
     2401 cctacaggac ttacgcaaaa attatgaatt ttcgtatata aatgaaaatc aattaaaaga
     2461 taatcctttg gaacagtttt caatttggat gaaagaagct attaacagtg ctgaattaga
     2521 accttctgcc atggttttat ctactgttga tgcacaaggt tttcctcatt cacgtatggt
     2581 gcttttgaaa gacttttcag agtcaggatt tgtgtttttt tctaattatg aaagcgacaa
     2641 agctattcaa atgaaaagca atcatgctgt ttctatttta tttttctggc aacaccttga
     2701 acgtcaagta cgtatagaag ggattgtgga aaaaattgca gaaattgaat cagatgctta
     2761 tttcttatct cgtccaatag agcataaatt gggtacatgg gcatcagcac aaagtaaacc
     2821 gattccttct tctacatttt tagaagaaca atttttatat tttaaagata aatttaaaca
     2881 cgatattcct cgaccacctc attggggtgg atatagtatt aagccatctc gttttgaatt
     2941 ttggcaaggc agacctaatc gtttacatga tcgttttgta tacaagttaa atgaatctga
     3001 ttggactatt gaacgtttag ctccttaatt atcgtttaaa aaaattgttt tatgcataaa
     3061 tacttcgttt ttatacttat tcttggctta ttgcaaaact tatctgcaca agcagcgtct
     3121 tttcaagcaa aattggatag tgtgatttat gttaaatcac aaaaaaccac gtatgtatat
     3181 gacactgatg acagaataga atacatgcta ctacaaggat gggtggaaca tcaaaacaaa
     3241 tgggattatc caactaaaac aagctatgaa tacgacaata caaatagatt agtactctca
     3301 ttttattcga catggaatct tagttcgcag aactatcaaa ttcaatcgaa aagaagttat
     3361 agctatactg aagctggtac tattttccaa tctatagctt ctgtttggtc taacaat
//
LOCUS       DAOSMY010000162         3393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_28046_length_3393_cov_3.341821, whole genome shotgun sequence.
ACCESSION   DAOSMY010000162 DAOSMY010000000
VERSION     DAOSMY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28046_length_3393_cov_3.341821"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLD76_04490"
     CDS             complement(<1..530)
                     /locus_tag="PLD76_04490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU27294.1"
                     /translation="MNRNEAELKFWVEHLKTAKTDYEVKQVYPEFARFINEKIGRDCG
                     VLDLGSGAYNTLGNRAGDIVLGVVCTDLLAKEYAELMHPKFCEVLYADMRDLKFTENR
                     FKVVHCSNALDHCEEPFVAIKEMERVGEWVFLRHFKNVGKIEQYQGMHEFDIYMNDDG
                     KMVIPDRGGNMQVYPE"
     gene            complement(523..1038)
                     /locus_tag="PLD76_04495"
     CDS             complement(523..1038)
                     /locus_tag="PLD76_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU27295.1"
                     /translation="MDVKTKNSLDIGCGDRMRIADGCMGYGVDLHVMGTNIFNLDCFV
                     ENLPIEDNAIDYVFAYDFLEHVPTVVYYSDKLFNCRVHLMNEVWRVMKDGAVFESNTP
                     YFNPDNVRQEWCQDPTHTIPWSKVTFNYFSDLGGEWDRLRSIYGIKARFKIVEMRDNG
                     SHLFVKLQKNE"
     gene            complement(1022..1684)
                     /locus_tag="PLD76_04500"
     CDS             complement(1022..1684)
                     /locus_tag="PLD76_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HOU27296.1"
                     /translation="MKIAVYTSIIGDFDELKTQPVQSVNCDFICFTDNDNVKSDFWRV
                     VHTDFVGNTSRFKAKYPKCFPHQLLWDYDITYWIDGSVLLKKSSTLEELIQLMGQNEN
                     LYFKHPDRDCIYDECEVSMNMPKYKGHSLREQVSKYKKAGYPEHNGLVACTTILRKNT
                     MNNRTLNEAWYNEMSDDLIQDQLSLPYVSWKAGVKINAVDINLWNNEYVLFGSHKNGR
                     QD"
     gene            complement(1734..2168)
                     /locus_tag="PLD76_04505"
     CDS             complement(1734..2168)
                     /locus_tag="PLD76_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27297.1"
                     /translation="MYELVKNNDIEDITKRVLALINGVSQIMNSGKELEETRMELARL
                     MVVVGEFEARAEYEQNSLDVQRKVQEAQAFANAKRTVGKISIAEANNYAEIEVSEIKK
                     KIVEAQYMYRQLRNLREAIVETNNAIAMRVRTLEREFQDTRS"
     gene            complement(2143..2364)
                     /locus_tag="PLD76_04510"
     CDS             complement(2143..2364)
                     /locus_tag="PLD76_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27298.1"
                     /translation="MKVYGYKEAWRKSWDKATDEDRRKTLKLPNWDNAIFLEITGIDV
                     EKELAVADNATRETVIKSLKSNVRACKKQ"
     gene            complement(2631..3134)
                     /locus_tag="PLD76_04515"
     CDS             complement(2631..3134)
                     /locus_tag="PLD76_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27299.1"
                     /translation="MRLNYSILNKANDKQALLDYWNGVEGFKNEATTRGTELHEKIKD
                     TKFKLSFMKQCKWEYHFEFVDDVHVVSGYIDCMDKDYLVDWKRSKNHAGKQDATQLYF
                     YDWCLRMCDLKGRKYGLMVQVDGDMNILSKAMYELGKDMGKWYVERRDKILEYYETAK
                     KDKLIKT"
     gene            complement(3131..>3393)
                     /locus_tag="PLD76_04520"
     CDS             complement(3131..>3393)
                     /locus_tag="PLD76_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27300.1"
                     /translation="ERAMREVGEQTLQDMDLEKFETEQYKIQKIKRGTKKPANGLKEG
                     MIGVKQELVQDTKALSDYVKENGRLPDGWVEVVSEYIKFEMK"
BASE COUNT          893 a          854 c          449 g         1197 t
ORIGIN      
        1 tattccgggt atacctgcat gttacccccc ctgtcgggta ttaccatttt tccatcgtca
       61 ttcatatata tatcgaactc gtgcattccc tgatattgtt ctattttccc aacattctta
      121 aagtgcctca aaaataccca ctctcccact ctctccattt ctttaatcgc cacaaaaggc
      181 tcctcgcagt gatctaaagc attgctacaa tgaacaactt taaatctatt ttcagtaaat
      241 tttaaatctc gcatgtcggc gtaaagcacc tcacaaaact tgggatgcat cagttcagca
      301 tactctttgg caagtaagtc ggtacaaaca acacccaata cgatatctcc agccctgttg
      361 cctaatgtgt tatatgctcc gctacctaag tctaacacgc cacagtcccg acctattttc
      421 tcgttgatga acctagcaaa ctctggatat acctgcttta cctcataatc tgttttggcc
      481 gttttgaggt gttccaccca aaactttaac tctgcctcgt ttctattcat ttttttgtaa
      541 tttaacaaat aaatgacttc cattatctcg catttcaaca attttaaacc tcgccttaat
      601 tccgtaaata cttcttaatc tatcccattc gccgcccaaa tcggaaaaat aattaaacgt
      661 aaccttgctc catggtatcg tatgtgtagg gtcttgacac cactcctgcc ttacgttgtc
      721 aggattgaaa tacggtgtat tactttcgaa tacagcccca tctttcatta ccctccaaac
      781 ctcgttcatt aagtgtaccc tacaattaaa taacttgtcg ctgtaatata caactgttgg
      841 tacgtgttct aaaaaatcgt acgcaaacac ataatcaatc gcattgtctt ctataggcaa
      901 gttttccaca aaacagtcca aattaaaaat gttcgtaccc ataacgtgta aatctacacc
      961 ataccccata caaccgtccg ctattcgcat tctgtcacca catccaatat ctaaggaatt
     1021 tttagtcttg acgtccattt ttatggcttc caaataatac atattcgttg ttccatagat
     1081 ttatatctac agcgttaatt ttaacccccg ctttccatga tacatatggt agcgacagct
     1141 ggtcttgaat caaatcgtca ctcatttcgt tataccatgc ttcgttaagt gttctgttat
     1201 tcatggtatt ttttctcaaa attgttgtac acgcaaccaa tccgttatgc tcgggatatc
     1261 ccgctttttt atattttgag acctgttctc taagtgagtg tcccttgtac tttggcatgt
     1321 tcattgatac ttcgcactca tcatatatac aatccctatc ggggtgtttg aagtacaggt
     1381 tttcgttttg ccccatcaat tgtatcaact cctccagcgt actactcttt tttaaaagta
     1441 cgcttccatc tatccagtac gtgatatcat aatcccacaa gagctggtga ggaaaacatt
     1501 taggatactt tgccttaaat cttgacgtgt tgccaacaaa atcggtgtgt acgaccctcc
     1561 aaaaatcact tttgacattg tcattatcgg taaagcatat aaaatcacaa ttcacagatt
     1621 gcacaggttg ggtctttaac tcgtcaaaat caccaattat tgaggtgtaa accgctatct
     1681 tcatattaac acaaaaaata tatgcactaa ttatagcaca acaatgcagg atatcaagag
     1741 cgtgtatcct gaaactctct ctcaagagtt ctaaccctca tcgctatagc attattggtc
     1801 tctacaatgg cttccctcaa gttccttaac tgcctataca tgtattgagc ctcaactatt
     1861 tttttcttta tttcgctaac ctctatttca gcatagttat tagcctcagc tatgctgatt
     1921 ttccctactg ttcttttggc gtttgcaaaa gcctgtgctt cttgtacctt tctttgaacg
     1981 tcaagtgaat tttgttcgta ttccgcacga gcctcaaact caccaactac aaccatcagt
     2041 cttgcaagct ccatccttgt ttcttccagt tcttttccac tattcataat ttgggataca
     2101 ccattaataa gtgccaaaac ccttttggtt atgtcctcta tgtcattgtt ttttacaagc
     2161 tcgtacatta ctctttaatg attttataac cgtttcacgt gtcgcattgt ctgcaacagc
     2221 caactctttc tcaacatcaa tgcccgtaat ttctaaaaat atcgcattat cccagttagg
     2281 caacttcaat gtttttcttc tgtcttcgtc agtagcttta tcccaactct ttctccacgc
     2341 ttccttgtat ccataaactt tcaaatatcc cccggtaatc ttgtctttgt tgaggttcta
     2401 aagtactcaa gtctctgttg ccaagcctta tcttaggagc cacaagtccc aaaacatacc
     2461 gcacaaccgc ataagatacg ccatacttcc ttgatattgc tctgggattt tgtgtcttaa
     2521 ttatcctttg taacttttga tactctggat agacattaac atattcaaga tattcattaa
     2581 taatgtcttg cttttgctct tcacttagag ctgacttcct acctaccacg tcatgtttta
     2641 attaatttat ctttcttggc agtttcataa tattctaaaa tcttatccct acgctctaca
     2701 taccacttac ccatatcttt acctaactcg tacatagcct tgcttagtat attcatgtcg
     2761 ccatctactt gtaccatcag tccgtatttc ctgcctttca gatcgcacat tctcaaacac
     2821 caatcgtaaa aatacaactg tgttgcatcc tgcttacccg cgtgattttt gctacgcttc
     2881 caatcgacaa gataatcttt atccatgcag tctatgtaac cacttaccac atgaacatca
     2941 tctacaaact caaaatgata ctcccattta cactgcttca taaacgacag cttaaacttc
     3001 gtatccttta tcttctcgtg tagttctgtt ccccttgtcg ttgcttcgtt tttaaatcct
     3061 tctacaccat tccaatagtc aagtagagcc tgtttatcgt ttgccttgtt aagtattgaa
     3121 tagttaagtc tcatttcatt tcaaatttta tgtattcact aacaacttct acccacccat
     3181 caggtagcct tccattctct tttacataat cacttaaagc cttagtgtct tgtaccaatt
     3241 cctgtttaac tcctatcatt ccttctttta acccattagc aggtttctta gttcctctct
     3301 tgattttctg tattttgtat tgttctgttt caaacttctc tagatccata tcctgtaacg
     3361 tttgctctcc tacctctcgc attgccctct cat
//
LOCUS       DAOSMY010000163         3348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_28527_length_3348_cov_3.182812, whole genome shotgun sequence.
ACCESSION   DAOSMY010000163 DAOSMY010000000
VERSION     DAOSMY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28527_length_3348_cov_3.182812"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLD76_04525"
     CDS             <1..423
                     /locus_tag="PLD76_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOU27301.1"
                     /translation="TLFRVMKILQLPYNQFEQQYRRTLFNVIARNHDDHVKNFSFLMN
                     KEGRWQIAPAYDITYQYKDGGTWTNVHQSSINGKFDKFSKEDLLGLGKTFGIKKANHI
                     LEEIIVAVGLWNKVAKELDIPTQTIEKINNNLRMNDFI"
     gene            complement(610..2067)
                     /locus_tag="PLD76_04530"
     CDS             complement(610..2067)
                     /locus_tag="PLD76_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOU27302.1"
                     /translation="MSNSSLKDKMIGALAWSSVDRVAQQGIQFIFGIILARLLAPDDF
                     GLLGMIMIFATLSYVLVESGFSAALIRKEDANETDYNSVFYVNLAISVALFVLLFFTI
                     PYFAEFFKQPALVSTARITFIAVIFNAFYLVPYVQVNRAMDFKTLAKINLFATLCSGS
                     IGITLAILDYGVWALVYQQIGYQFFRGIGFYALVRWIPSFTFSLDSIKHLWKFSMHIL
                     GSSLLTIGFNNLYIFLLSRFYPIKQVGYYTQANKMSETANFTFQAILASSTYNLFTQI
                     QADKERLLRVYRQLIQHISLVVIPVSLVLMVVAKPLFFVLFSEKWLPAVPYFQLLCVA
                     NVFAPLYLVNMNVLNSLGESKRTFYIEIIKRLFILISIVVCFQFGIQALLAGYAIACT
                     MAFVFSTIYIKKHLRHYFFHQLNDVIMPIMIGISIALIAWSMNSLISNLYLLLVIQLL
                     LALVVYIIILRFFCKEKLYGFYDYIKRQSKQLTKK"
     gene            complement(2090..>3348)
                     /locus_tag="PLD76_04535"
     CDS             complement(2090..>3348)
                     /locus_tag="PLD76_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU27303.1"
                     /translation="LPLELPEIDKYLPTETGEPPLGRAKAWHTAEGYPYELNTMPGFA
                     GSSAYFLRYMDPRNSNELVSKEANNYWQQVDLYLGGTEHATGHLIYSRFWNKFLFDLG
                     LICKDEPFKKLVNQGMIQGRSNFVYRIKDTNTFVSYHLKDQYEVTPIHVDVNMVSNDI
                     LDIERFKAWNPEYNKAEFILEEGKYVCGWAVEKMSKSMFNVVNPDDIVEKYGADTLRL
                     YEMFLGPLEQSKPWDTNGIDGVHRFLRKLWSLFYKDGNYIVTQDTPTTEELKTLHKTI
                     KKITFDIENFSFNTSVSAFMICVNELAQQKCSKQAILEPLVILIAPFAPHIAEELYHA
                     LGHTTTVCDAVFPICNESYLVESTINYPISFNGKVRFTLPLEASLSKEEVEKAALQNE
                     QTRKFLQDKQPKKIIVVPGKIVNIVL"
BASE COUNT         1156 a          600 c          564 g         1028 t
ORIGIN      
        1 acattattta gagttatgaa aatacttcaa cttccttaca atcaatttga acaacaatac
       61 agaagaactc tttttaatgt aattgcaaga aatcatgatg accatgtaaa gaatttctct
      121 tttcttatga acaaagaagg aagatggcaa attgcaccag cttatgatat tacataccaa
      181 tataaagatg gtggaacatg gacaaatgtt caccaatcat ctataaatgg gaaatttgat
      241 aaattttcaa aagaagattt gctgggttta ggcaagactt ttgggattaa aaaagcaaat
      301 catattttag aagaaataat agtagcagtt gggctttgga acaaagtagc taaggaatta
      361 gatataccta cacaaaccat cgaaaagatt aataataatt tgagaatgaa tgattttatt
      421 taatgaaatc tactgctaga tgaattattg ctaacttctt ctatagaata tctttacacc
      481 acataaaaaa aatattcttc cgtgcaatct ccgtgccact ctgtgaaata atctcacaag
      541 aagaagcatg acccactaaa cactcaacag tacacacgct gttcaacctt cataaacgct
      601 cctcattttt tactttttcg tcagttgttt tgattgtcgt tttatgtaat cgtagaatcc
      661 atatagtttt tctttgcaga aaaaacgcaa aataataatg tacacaacca aagcaagtag
      721 tagttgaata actagtaaca gatataggtt gctaataagt gaattcatac tccatgcaat
      781 aagagcgata gaaataccta tcatgatagg cattatgaca tcatttagtt gatgaaagaa
      841 atagtgtcgc aaatgttttt ttatgtaaat ggttgaaaaa acaaaagcca tggtgcaagc
      901 tatagcgtat cctgctaata atgcctgaat gccaaattga aaacaaacaa cgatagagat
      961 aagaatgaat agtcgcttga ttatttcaat ataaaaggtg cgtttcgatt cgcccaatga
     1021 atttagtaca ttcatattta ctaaatacaa gggagcaaaa acattggcta cacacaacaa
     1081 ttgaaaataa ggcacggcag gcaaccattt ttctgaaaat aatacaaaaa acaaaggttt
     1141 agcaactacc attaatacta gagagacggg tattaccaca agcgatatat gttgtatcag
     1201 ttgtctgtac acacgaagta atcgttcttt gtcagcttgt atttgtgtaa ataagttgta
     1261 cgtactactt gccaaaatag cttgaaaggt aaaattcgct gtttcactca ttttatttgc
     1321 ttgtgtataa tatcctactt gtttgatagg ataaaaacga cttaacaaaa agatgtataa
     1381 attattaaaa ccaattgtca gcaatgatga gcccaatatg tgcatgctaa atttccataa
     1441 atgtttgatg ctatccaaag aaaaagtgaa agagggtatc catctcacca aagcataaaa
     1501 cccaattcct ctaaaaaatt gatagccaat ttgttgatat actaatgccc atacgccata
     1561 atccaatatt gccaaagtta taccaatgct accactacaa agggttgcaa aaagattgat
     1621 tttagccaaa gttttaaaat ccattgctcg attcacttgc acgtatggaa ccaagtaaaa
     1681 agcattaaaa atgaccgcga taaatgtaat gcgagcagta gaaaccaatg caggttgctt
     1741 aaaaaattca gcaaaatagg gtatagtaaa aaacaaaagc acaaacaatg caacagaaat
     1801 tgccagattg acataaaaaa ccgaattgta atcggtttcg ttggcatctt ctttgcgaat
     1861 tagggcagca ctaaaaccgc tttctaccaa cacatacgag agggtagcaa atatcataat
     1921 cattcctaac agaccaaaat catcaggagc caaaaggcgg gcaagtatga taccaaaaat
     1981 aaattgaatg ccttgttgag caactctatc tacagagctc cacgccaagg caccaatcat
     2041 tttatctttc aaactactgt tgctcattta gcttttggtt aggcggtcgt tatagtacaa
     2101 tatttacaat tttacctggt accacaataa tttttttggg ctgcttgtct tgcaaaaatt
     2161 tacgtgtttg ttcgttttgc aaggctgctt tttcaacttc ttcttttgat agagaagcct
     2221 ctaggggcaa ggtaaaacgt acttttccat taaatgaaat agggtaattg atagttgatt
     2281 ctaccaagta actttcgttg caaattggaa aaacagcatc acaaacagta gttgtatgcc
     2341 ccaaagcgtg atacaattct tctgcaatgt gcggagcaaa tggggctatt aaaattacca
     2401 atggttctaa aatagcttgt ttgctgcatt tttgttgtgc caattcgttt acacaaatca
     2461 taaatgccga cacagaggtg ttaaacgaaa aattctctat atcaaaggta atttttttaa
     2521 tcgttttgtg caaggttttt agttcttctg tagttggtgt atcttgcgtt acaatatagt
     2581 ttccatcttt gtaaaacaac gaccataatt tgcgtaaaaa acgatgaaca ccgtcaatcc
     2641 cattggtatc ccaaggtttg gattgttcca acggacccaa aaacatttcg tacaaacgca
     2701 aggtgtcagc accatatttt tctacaatat catcggggtt tacaacgtta aacatcgatt
     2761 ttgacatttt ttctactgcc caaccgcata catatttccc ttcttcaaga ataaattcag
     2821 ccttgttgta ttcaggattc catgctttaa atcgttcaat atcaagtatg tcgttgctca
     2881 ccatattcac atctacgtgt attggggtaa cttcatattg gtcttttagg tgatatgaaa
     2941 caaaggtgtt ggtatctttt atgcgataca caaaattact tcgtccttgt atcattcctt
     3001 gattgactaa ttttttaaaa ggctcatctt tacaaattaa tcctaggtcg aataaaaatt
     3061 tgttccaaaa acgactataa attaaatgcc ctgtagcgtg ttctgtacca cccaaatata
     3121 aatctacttg ttgccaatag ttattggctt ctttgcttac caattcgttg ctattacgag
     3181 gatccatgta gcgtaaaaag tacgccgaag aaccagcaaa accaggcatg gtatttagct
     3241 cgtagggata accttcggct gtgtgccaag ctttagccct tccaagcgga ggttcgcccg
     3301 tttcggtggg taaatatttg tcaatttcgg gcaattccaa aggcaatt
//
LOCUS       DAOSMY010000164         3342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_28597_length_3342_cov_3.209614, whole genome shotgun sequence.
ACCESSION   DAOSMY010000164 DAOSMY010000000
VERSION     DAOSMY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28597_length_3342_cov_3.209614"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /gene="yidC"
                     /locus_tag="PLD76_04540"
     CDS             complement(<1..1665)
                     /gene="yidC"
                     /locus_tag="PLD76_04540"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU27304.1"
                     /translation="MDKNTLVGFLLIIGIFIGFFWISQPSAEQLEQRKRYNDSIILVQ
                     KEQSKLAEIEAIQQENVSTTVDNVPLDSVVDVNNAFGPFAKFTQGNNELLVLENKKLK
                     IGVDTKGGVIQTVELKEYLTHDKQTLQLMDGSSEKTNFTFVTANNRIVNSEDLYFQPI
                     WTNHGQSLIMRLAFDSISYIDFEYALKEDDYIVSFKITPHNVQHILSKRLTALDMEWA
                     THIPQQELGRKFENRYAAVYFKYFGDKDVEDLKADKNDERTIPNRLHWIASKGQFFSS
                     VLIADDYFMSSTLQSNLEPDTSRYLKSTKATMSVDFDTKGVKSTGFTYYFGPNKYKLF
                     KSYDKGKEESNKWDLDELIPLGWGIFGWVNKILVIPVFNFLEKFIDSYGIIILLLTLF
                     IKLVLLPLTYKSYLSTAKMKVLRPQIEEINAKIPAEKAMERQKATMALYKKVGVSPMG
                     GCLPMMLQMPILFALFTFFPASIELRQESFLWAKDLSTFDAIVSWDTYIPIISTYYGN
                     HISLFCVLMAITNIVYTKLNSDATGSTQQMPGMKYVMYLMPLMFLVF"
     gene            complement(1678..3279)
                     /locus_tag="PLD76_04545"
     CDS             complement(1678..3279)
                     /locus_tag="PLD76_04545"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU27305.1"
                     /translation="MSKTKYIFVTGGVTSSLGKGIISASLGKLLKARGYNVTIQKFDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNVQTTRENNITTGRIYQNVIK
                     KERRGDYLGKTVQVIPHITNEIKQNIKQLGTKSKFDFVITEIGGTVGDIESLPFIESV
                     RQLKWELGKDCLCIHLTYVPYVAAAKELKTKPTQHSVKQLLELGVQPDVLVLRTEHPL
                     SQEIKEKVALFCNVAKSAVIECRDVPLIYEVPLRMQEEGLDEIVLSKMGLEDVQPANL
                     TQWTSFLTKLQTATEIVRIGLVGKYVELPDAYKSIIESLIHASAYNERKLKLELIQSE
                     KLTAENVTKTLSGLDGILIAPGFGDRGIEGKFIACQYARENNIPTLGICLGMQCMVID
                     IARNMLGYTDANSTEFNERTSNNVIDMMEQQKGLSVKGGTMRLGAYDCELEKHSLAYS
                     IYGKKHIQERHRHRYEFNNQYKQVFEDIGVKFSGINPDTNLAEIIEIPSLKWYIGVQF
                     HPEYSSTAETPHPLFINFVKAAISK"
BASE COUNT         1077 a          614 c          454 g         1197 t
ORIGIN      
        1 gaaaactaaa aacataagtg gcattaaata cataacatat ttcataccag gcatttgttg
       61 tgtagatcca gtagcatcac tattcaattt tgtatataca atatttgtaa tagccattaa
      121 tacacagaat agacttatgt gattaccata atacgtagaa atgattggaa tataagtatc
      181 ccaactcaca atagcatcaa atgtagacaa atcttttgcc cataagaaac tttcttgcct
      241 caactcaatt gatgctggga aaaaagtaaa caatgcaaat aaaataggca tttgtaacat
      301 cattggcaaa catcctccca taggacttac tcctaccttt ttgtacaaag ccatagtggc
      361 tttttgtctt tccattgctt tttctgcagg tatcttagca ttaatttctt caatttgagg
      421 gcgaagtacc ttcatctttg ccgttgacaa atatgactta taggtaagag gcaacaacac
      481 taatttaata aaaagggtaa gtaataagat aatgatacca tagctatcaa taaatttttc
      541 caaaaaatta aatacaggta taaccaaaat tttattaacc catccaaata taccccaacc
      601 taaaggaata agttcatcta aatcccattt attcgattct tctttccctt tatcatagga
      661 tttaaaaagt ttatatttat taggaccaaa ataataggta aaacctgtac tcttaacccc
      721 ctttgtatca aaatctacac tcattgttgc tttagtagac tttaaatacc gagaggtatc
      781 aggttccaaa ttagattgta atgtagatga cataaaataa tcatctgcaa tcaacacaga
      841 ggaaaaaaat tgacctttac ttgcaatcca atgtagacga ttaggaattg ttctttcatc
      901 atttttatct gctttcaaat cttctacatc tttatctcca aaatatttaa aatatacagc
      961 agcatatcga ttttcaaact ttctacctaa ttcttgttga gggatatgag tagcccattc
     1021 catatctaac gcagttaagc gttttgatag tatatgttgt acattatgag gtgttatttt
     1081 gaaagaaact atataatcat cttcttttaa tgcatattca aaatcaatat aagaaattga
     1141 atcaaaagcc aaacgcataa tcaaggattg accgtgatta gtccatattg gctgaaaata
     1201 caagtcttct gagtttacaa tacgattatt tgcggtaaca aacgtaaagt ttgttttctc
     1261 actactccca tccattaatt gtagcgtttg tttatcatga gttaaatact ctttcagctc
     1321 aacagtttgt attacacctc cttttgtatc aacaccaatt tttaactttt tattttctaa
     1381 tactaacaat tcattgttac cttgtgtgaa ttttgcaaat ggtccaaatg cattgtttac
     1441 atctactact gaatctaagg gcacattatc tacagtagta cttacatttt cctgttgtat
     1501 agcctctatc tcagctaatt tcgattgttc tttttgtact agtatgattg aatcattata
     1561 acgtttacgt tgttctagtt gttctgctga tggttgtgaa atccaaaaga acccaataaa
     1621 aattccgata ataagtagaa aacctactag tgtatttttg tccatattgt caagttatta
     1681 tttgctaata gctgctttta caaaatttat aaataaaggg tgtggagttt cggcagtact
     1741 actgtattca ggatgaaatt gtactccaat ataccatttc aaagatggga tttcaatgat
     1801 ttcagccaaa tttgtatctg gattgatacc tgaaaatttc actccaatat cttcaaatac
     1861 ttgtttatac tgattattaa attcataacg atgtctatgt cgttcttgaa tatgtttctt
     1921 gccatatata ctatatgcca acgaatgctt ttctagctca caatcatatg cccctaaacg
     1981 cattgttccc cctttaacgg acaatccttt ttgttgctcc atcatatcaa taacattgtt
     2041 tgatgtacgt tcgttaaatt cagtagaatt agcgtctgta taaccaagca tatttcttgc
     2101 tatatctatt accatacatt gcattcctaa acatataccc aatgtaggaa tattattttc
     2161 acgtgcatac tgacaggcaa taaatttacc ttcgattcct ctatcgccaa aacctggagc
     2221 aatcaaaata ccatctaatc cactcaaagt tttagttaca ttttctgctg tcaatttttc
     2281 cgattgaata agttctaatt ttagtttccg ttcgttatat gctgatgcat gtattaatga
     2341 ttcaatgata gatttatagg catcaggcaa ttctacatat tttccaacta gccctattct
     2401 cactatttca gtagcggttt gtaattttgt taaaaaactt gtccattgtg ttaaattagc
     2461 aggctgtaca tcctccaatc ccattttaga aagaacaatc tcatctaatc cttcttcttg
     2521 catacgcaat ggtacttcat aaattaacgg tacatcccta cactctatta cagcagattt
     2581 tgctacatta caaaataaag caactttttc tttaatctct tgtgataaag gatgttcggt
     2641 tcgtaacacc aaaacatctg gttgtacacc taattcaagc agttgtttta cagaatgctg
     2701 tgtaggtttc gtttttaatt ccttagctgc tgccacatat ggcacatacg taaggtgtat
     2761 acataaacaa tccttaccta actcccactt taactgacga acactttcta taaaaggtag
     2821 agattctata tctcctactg tacctccgat ttccgtaata acaaaatcaa atttactttt
     2881 tgttccaagc tgtttaatat tttgtttaat ctcattcgta atatgaggga taacttgaac
     2941 tgttttacct agatagtctc ctctacgttc tttttttata acattttgat agattctacc
     3001 agttgtaata ttattttctc tggtagtttg aacatttaaa aaacgctcat aatgacccaa
     3061 atctaaatcg gcctcatgac catcaacggt tacataacat tctccatgtt cgtatggatt
     3121 taatgtaccc ggatcaatgt ttatgtatgg atcaaatttt tgaatggtta cattgtaacc
     3181 tctggctttt aatagttttc ctaaggaagc agatatgatt cctttaccta aagatgatgt
     3241 aacaccacct gtaacaaaaa tatacttcgt ttttgacaca attatagcta ttagattagt
     3301 tttttaatgg gtgcaaattt acaaaaaata aaataggttt tg
//
LOCUS       DAOSMY010000165         3339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_28626_length_3339_cov_2.505177, whole genome shotgun sequence.
ACCESSION   DAOSMY010000165 DAOSMY010000000
VERSION     DAOSMY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28626_length_3339_cov_2.505177"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLD76_04550"
     CDS             <1..342
                     /locus_tag="PLD76_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOU27306.1"
                     /translation="VIDATTHTFQVQIKISNAGGVLRPGMFARIGVDFDKINAKVVPY
                     QAVLKLQGSNERYVYVNKNGVAKRVKVQMGKRYDDKIEILSDELKEGDQLVVVGQARL
                     VDGVKVNVVNE"
     gene            358..3339
                     /locus_tag="PLD76_04555"
                     /pseudo
     CDS             358..3339
                     /locus_tag="PLD76_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1110 a          476 c          708 g         1045 t
ORIGIN      
        1 gtgatagacg ctactacaca tacttttcag gtgcaaatta aaatctcgaa tgctggcgga
       61 gtattacgac caggaatgtt tgcacgtatt ggagttgatt ttgataaaat taatgctaaa
      121 gtagtacctt atcaagcagt cttaaaacta caaggctcta acgaacgcta tgtatatgtt
      181 aataagaatg gagtagcgaa acgtgtaaaa gtacaaatgg ggaaacgcta tgatgataaa
      241 atagaaattt tatctgatga actcaaagag ggagaccaat tagtggtagt gggacaagct
      301 cgattagtag atggtgtaaa agtaaatgtt gtaaacgaat agaaatttaa caataccatg
      361 agtatatata aatcatctgt aaataagcct attacaacaa tattgatttt tgttggggtt
      421 attattatgg ggttgtattc actaatacaa ctaccgattg atcagtttcc ggaaatagaa
      481 ataccgtcag taacggttat gacttcgtat cctggagcaa acggaacgga agtagaaaca
      541 aatgtgagca aggtcttgga aaatagcctt aactctgttg atgggcttaa agaaatttat
      601 tcaacatcca aagataattt gtcgttagta acacttgagt tcgaatgggg agcgaattta
      661 gatgaggcac ttaatgatgt gcggtcttct atcgaccttg tgtatgatgc tcttccagat
      721 ggagcttcac gacctatgat tatcaagctt aataccagca tgatgcctgt tatgcaatat
      781 gctgtaactg ccgaagatag ctatcctggt ataaaaaaaa taatcgaaga taacttaata
      841 accgtattaa atagaataga tgggatagga aatatctctt tattaggatc ccctcagcgt
      901 tatgtatatg tagatataga tccaacgaaa ctaaattcat tcggattaag cttagaaacg
      961 gtaggttctg ctattgccaa taacaacatg aatttggctt caggtaccgt taaaatggga
     1021 aaagaacaat atcaattgcg tgtacaagga gaatatgtag caagcgaaga tattaataat
     1081 attgttgttt cgtcaacgta tgatggtaag caaatatttg taagagatgt agctcaagtt
     1141 agagatacta ttaaagatgt taccttagaa gaaaagatta acggaaaaga tggagcccga
     1201 ttgattatta tgaaacaatc tggtgccaat acggtagaaa ttgctaaaaa agtacgcgaa
     1261 caaattgctc aagtacaaaa aacattgcct cctgatattc attttgaaat tatttatgac
     1321 tcttctgaac ttatacaaaa tgcagttttt ggattgtcag aaacaatcat gtatgcactt
     1381 atttttgtgg tatttgttgt acttttcttt ttaggtaaat ggagagcaac atttattata
     1441 tcacttacta ttcctatatc attaatcgtt tcatttattt gtattacaca ggaagttcgt
     1501 taaatattat ctcactttcg tcgttatcca tagctattgg tatggtggta gatgatgcaa
     1561 ttgtggtgct cgaaaatata aacaaacata tcgaaagagg ttctagccct cgtgaggcgg
     1621 ctatttacgc taccaacgag gtgtgggttt ctgttatcgc tactaccttg gtaattgtag
     1681 ccgtattttt accgttaacc ttgataggag gtattgctgg tgtgatgttt aaagaattag
     1741 gttggattgt aaccattgtt gtatgtgttt caactttggt agctattacg ctaactccta
     1801 gtttgtctgc tatgatgtta accatcaaaa agaaaactga aaatagtaaa acaaatcgtt
     1861 ttagttatca aaatacagta gtaaaatggc ttgataaagt agatgaacta tatgcaaata
     1921 ttttacgctg gtgtttacga aataaggcta ttaccttaat tgtagctgta ctcatattta
     1981 taggttcgtt ggtacctgtt tttgtgggag ttattggtac tgattttatg ccacaaaatg
     2041 atgaaggaag gcttaccgta acaattgaat tacaacgagg tacacgcttg gaggaagcaa
     2101 gtaaaacagc acgtataata gaaagcagga ttgccaagat tgctccagaa atacaattaa
     2161 tatcgacgtc aacaggttcg tctgatgaaa caggtttttc ttctctattt acttcttcta
     2221 ccaataataa aattgttatg atggttagga catctaaaaa atatgataga gaaagaacga
     2281 tatttgaaat tgctgaaaaa attcgagaag acttagctac cttacccgaa gtaataacat
     2341 acgatgtatc tactgcacgt tttggcggtg gtctggttgg taataatgtt gaagtagaga
     2401 tttttggtca tgattttgat gcaactaatg cagtggcggc agaattgaaa caacgaatgg
     2461 aaaaattatc aggtgctcgt aatgttgata ttgatagaga agaagataga ccagaattaa
     2521 aaatagagct tgataaagaa aaaatatctc gccacggcat taatacagtg atgatggcat
     2581 cttatgttcg caatagagta aatggtatgt cagctggtaa ttttaaagag cagggagatg
     2641 agtatcctat tttggttaga ttgacagagg agcatcgtaa tagtattact tctatcgaag
     2701 agttaaatat tgctacgatg caaggtaaat tattgaaatt aaaagagttg gcagatatta
     2761 aagaatattg gggaccacca aatattgaac gtaaacgtcg tgaacgtatt gtaaccgtat
     2821 ctgctacgcc agagggtgtg tcgctcggtg aattagctga acaaattaag gcagaggtaa
     2881 aacaaattag tgttccacaa ggagtacaag tgcaagttgg tggtgattat gaaacacaac
     2941 aggaatcgtt tgctgatatt gctaccttgt ttttattgat tgtaatttta gtatacattg
     3001 taatggcatc gcagtttgaa tctttctcca aaccatttat aattatgatg tccgttcctt
     3061 ttgctattac gggtgttatt ttggcattgc tgattaccgg gacatcactt aatttaatag
     3121 gggcattagg tgtggtacta ttggtaggta tcgtggtaaa aaatggtatt gtgttagtcg
     3181 actatatcaa tttaatgaga gatagaggaa atgagctaaa tgaagctatt gctttatcag
     3241 ggcagtcacg tttacgacca gtgttaatga ccgctgtaac tactattttg gggatgattc
     3301 cgatgacatt aagcactagc gagggctctg aaacatggg
//
LOCUS       DAOSMY010000166         3287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29220_length_3287_cov_3.982364, whole genome shotgun sequence.
ACCESSION   DAOSMY010000166 DAOSMY010000000
VERSION     DAOSMY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29220_length_3287_cov_3.982364"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLD76_04560"
     CDS             complement(<1..611)
                     /locus_tag="PLD76_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOU27307.1"
                     /translation="MKLNKLKRSLDLKDAVAVGLGAIIGAGIFVVTGVAAGVSGPSFI
                     IGLLIAGTIATFNGLSSSQLAAVYPQSGGTYEYGYRLLTPSLGFSAGWMFVLSKLSAG
                     GIVAIGFGSYFHQLIPYFSPIEYSIAAIVLLTFANLWGIQKVGTINIAIVLITLLSLL
                     YFVVGGIPSVDKTNFIPFAPFGWKGIAESAAILFFAFTGYARIA"
     gene            930..2309
                     /locus_tag="PLD76_04565"
     CDS             930..2309
                     /locus_tag="PLD76_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU27308.1"
                     /translation="MIQKHSPLSLGTESIGKLLAQYAIPAIVAMTASSLYNITDSVFI
                     GHGVGALAISGLALTFPVMNLAAAFGSLVGAGASALMSMRLGQKDYDTANKVLGNVFV
                     LNIIMGLAFTGIMLVFLKPILYFFGASEATLPYAYDYLQVILYGNVVTHMYMGLNALL
                     RSSGHPQKAMYATICSVILNLGLTPLFIYVFGWGIKGSAWATVLSQLLMLIWQITIFS
                     NKKNFIHLKKGVFRLEKKIVLDSFAIGLSPFFMNAASSVIIIVINQALSRHGGDLAVG
                     AYGIVNRVVFLFAMVVLGLNQGMQPIAGYNFGTQHYDRVDKVLRTTILYATGIMIFGF
                     IIGQFFPFSVASVFTTDVQLTEHAVKGLRIVMIFFPIIGFQMVTGNFFQSIGQAKKAI
                     FLSLTRQMIFLLPCLLIFPSIWGVKGVWFSLPTADLASSLVAGWLLSKQLKQFKRSKQ
                     TKSCDEVGI"
     gene            2336..2944
                     /locus_tag="PLD76_04570"
     CDS             2336..2944
                     /locus_tag="PLD76_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU27309.1"
                     /translation="MNNNFIITIGRELGSGGRIIGAQLAKKLNSAFYDKELILRASKE
                     SGLSSSFMEQVDEHFSPIQTGGLLGDFFQQFYFSSESCFQIQSDVIREIAEHESAVIV
                     GRGADYVLREHPRCLNIFVTANMSDRIQRVAESHTISEDEARILIEKADKKRASFYNY
                     FTSKEWGRAKSYDLCVNSSVFGLEKTTDYIFQLVKDRFSPIP"
BASE COUNT          985 a          529 c          674 g         1099 t
ORIGIN      
        1 gctatacgtg catatcctgt gaaagcaaaa aataagatag ctgcagattc ggctattcct
       61 ttccatccaa atggagcaaa aggaatgaaa tttgttttat caacacttgg aataccacca
      121 actacaaaat atagtagtga taataaggtg atgagtacaa tagcaatatt tatcgttcct
      181 actttttgta tgccccacaa attggcaaac gtaagcaata caatggctgc aatagaatat
      241 tcgatgggag agaaataggg tataagttga tggaaatagc ttccaaaacc tatagccaca
      301 atgcctcctg ctgatagttt gcttaataca aacatccatc ctgcagaaaa gcctaacgaa
      361 ggggttaaca agcgataccc atattcgtac gtacctccag attgaggata aacggcagcc
      421 aattgtgatg aactgagccc attaaaagtg gctattgtac ctgctatcaa taagcctatg
      481 ataaaagaag gtccagatac accagcagca actcctgtaa caacaaatat acctgctcca
      541 ataatcgctc ctaagccaac agctacggca tcttttaagt ctaaactcct ttttagttta
      601 tttagtttca tagtgtgtat gtttgtttaa ccctatcccc acaatatctg taagtataaa
      661 atcgttgtta tctatgtaca aaagtattga aaaagagaat aactatatgg tataattgta
      721 ctaaaaaatg gtatttttga agaatataca gtatggttgg ttgtgtgtac gatttttttt
      781 cgttgactag tatgctatgt ttgcccattc attttgaaaa taaaaaagga gatatcgtta
      841 cgttgaatat tctccgaaaa aagtataaaa aatcagtagt atttgtaaac ttttcacgaa
      901 ctttgtagtt cttccaaaaa aaaacaacaa tgattcagaa acattctcca ctaagtcttg
      961 gtacagaaag tattggtaag ctgttggcac aatatgctat tcctgctatt gtagcgatga
     1021 cagcttcttc tctatacaat atcacggata gtgtttttat tgggcatgga gttggagctc
     1081 ttgctatatc aggtttagct cttacttttc cggttatgaa cttagctgcg gcatttggtt
     1141 cgctcgttgg agcgggagct tctgctctta tgtcgatgcg attaggtcaa aaagactatg
     1201 acactgcgaa taaagtacta gggaacgtat ttgtgctgaa tattattatg gggttggcat
     1261 ttacaggcat catgcttgtt tttctgaaac ctattttgta tttttttggt gctagcgaag
     1321 caacactgcc ttatgcgtat gattatcttc aggtaattct ttacggaaat gtagtaacac
     1381 atatgtatat gggtcttaat gccttgttgc gttcatcggg tcatcctcag aaggcaatgt
     1441 atgcaacgat atgttcggtt attcttaatt taggacttac cccattattt atttatgttt
     1501 ttggatgggg tattaaaggg agtgcttggg ctactgtact gtcacaactc ttgatgttga
     1561 tatggcagat aaccattttt agtaataaaa agaattttat tcatttgaag aaaggagttt
     1621 ttcgattaga aaaaaagata gttctcgatt cgtttgccat tggattatct ccttttttta
     1681 tgaatgcagc gtcatctgtt attattatcg ttatcaatca ggcattaagc cgtcatggtg
     1741 gggatttggc agtaggtgct tatggaattg ttaaccgtgt tgttttcctt tttgccatgg
     1801 tagtattggg acttaatcag ggaatgcaac ccattgcagg gtataatttt ggtacccagc
     1861 attatgatcg tgtagataaa gtgctgcgta caactatttt gtatgccaca ggcattatga
     1921 tttttggatt tattataggg caatttttcc cattttctgt tgcatctgta tttaccaccg
     1981 atgtgcaact taccgaacat gcagtaaaag gcttgcgtat tgtgatgatt ttttttccaa
     2041 ttattggatt tcagatggta acgggcaatt tttttcaaag tattggtcag gcaaaaaaag
     2101 ccatttttct ttcacttact aggcagatga tatttttgtt gccttgtttg cttattttcc
     2161 cttccatatg gggcgtgaaa ggtgtttggt ttagtttgcc tacagctgat ctagcctcaa
     2221 gtttggttgc aggatggttg cttagtaaac aacttaaaca atttaaaagg agtaaacaga
     2281 ctaagtcgtg tgatgaagta gggatttaat tcaacgttat taggaggatt tttctatgaa
     2341 caacaatttt attattacca ttggtagaga attaggaagt ggaggtcgca ttataggagc
     2401 tcaattggcg aaaaaactga atagtgcgtt ttacgacaaa gaattaattc taagagcctc
     2461 caaagaaagc ggtctgtcta gctcttttat ggagcaagtt gatgagcatt ttagtcctat
     2521 tcagacagga gggcttttgg gcgatttttt tcaacaattt tattttagta gtgaatcttg
     2581 ttttcaaatc caaagtgatg ttattcgtga gattgctgaa catgaatctg ccgtaatagt
     2641 aggacgtgga gctgattatg tgttgagaga acatccgcgt tgtttgaata tttttgttac
     2701 agctaacatg agtgatcgta tacaacgtgt tgccgaatcg catacgataa gtgaagatga
     2761 agcacgtatc cttattgaaa aagccgataa aaagcgtgct tctttttaca attattttac
     2821 gagcaaagaa tggggcagag caaaatcata tgatttgtgt gtaaattctt cggtatttgg
     2881 attggaaaaa acaaccgact acatttttca gcttgtgaaa gatagatttt ctccaatccc
     2941 ctaatcatta agatgcatac ttgttagtga attgtagcta cacactatca aatatataat
     3001 aaaagaaaac aaaatttttg tcgatatcgt gtgagagggt ctggttgttt gtttttggtt
     3061 ggcttaaaaa aatctctgtt aagcagattg aaaatcctga tagaatgttc aaggtcttaa
     3121 aggtcggatt tggaaatccg acctgacggg gttgttctag tgttttattg tcgttacaaa
     3181 cgacaaacta taaacccctc gttgtgaaaa cgaggggtag ggagcagtag cctgtagccc
     3241 catattacta actaccatta ctgtagaacg tttacaaaaa cgtggtt
//
LOCUS       DAOSMY010000167         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29316_length_3278_cov_4.357431, whole genome shotgun sequence.
ACCESSION   DAOSMY010000167 DAOSMY010000000
VERSION     DAOSMY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29316_length_3278_cov_4.357431"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLD76_04575"
     CDS             <1..267
                     /locus_tag="PLD76_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27310.1"
                     /translation="NSTQFANQEKINPALYEEAFTIYGESEDKLYFTFNASGHACVYY
                     FNGSQYKIYKEVEFPKFNKNSEITIRRIDNDIYFYIDYFYLFSR"
     gene            complement(253..1599)
                     /locus_tag="PLD76_04580"
     CDS             complement(253..1599)
                     /locus_tag="PLD76_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOU27311.1"
                     /translation="MRQKKVAWKYRVPFLLLAIVAIVSLIPVDHETLIPLGGELLEVN
                     HADTSWLLMASALVLLMTPGLAFFYGGMVRYKNLVSTLLQSFIALGIISVLWIVVGFS
                     LAFGESVGGFIGNPTSFMFFKNVGLTPHPDFGPTIPFALFAMFQLKFAIITPALITGG
                     LAERVRFTSLMLFLILFSLLIYAPLAHWTWHPEGFLRKWGVLDFAGGTVVHISAGFAA
                     LSGAIFLGKRKGVNSEARPTNIPYILLGTGLLWFGWFGFNAGSSLAANDMAVRAFVNT
                     NISSATAMMTWLFVDGAMGKKRSAAGAAIGAVVGLVAITPAAGFVTVGQSIFFGFVAS
                     LVSYTAVQLKSKTKIDDTLDVFPCHGLGGIVGMLLTGVFAVENGVGVGLIHGNVTQFM
                     FYLLALLIVGVFTFGGSYLLYKVTNLILPMRVTAEQEEIGLDITQHGEEAFDLSTK"
     gene            complement(2009..2890)
                     /gene="folD"
                     /locus_tag="PLD76_04585"
     CDS             complement(2009..2890)
                     /gene="folD"
                     /locus_tag="PLD76_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOU27312.1"
                     /translation="MQLIDGKAIADIIKQEIAVEVATIKSMGQRPPHLAAILVGHDGG
                     SETYVAHKVKACEQCGFNSTLIRYEDTVTETELLAKISELNNDTAIDGFIVQLPLPKH
                     ISEQKIIEAIDYRKDVDGFHPINVGRTSIGLPAFVSATPAGIMELLKRYGIATAGKHC
                     VVLGRSNIVGKPVANLMIQKAYPGNATVTVCHSYSADLVEQCQKADIIIAALGQPNFV
                     KGNMIKDGAVVIDVGTTRVPSTTTKSGFKLTGDVLFDEVAPKCSYITPVPGGVGPMTI
                     VSLMRNTLLAAKKELFK"
     gene            complement(2898..>3278)
                     /locus_tag="PLD76_04590"
     CDS             complement(2898..>3278)
                     /locus_tag="PLD76_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU27313.1"
                     /translation="EEEARRVAKKIAKNQFDFNDFMGQIQQIKKMGNLKDLAAMIPGM
                     GKAMKDVDIKDDAFKGIEAMIQSMTPAERENPQLLNGTRKMRIANGSGTSIQEVNKLI
                     KQFDGIRKMMRMVQTNGNKFPKFK"
BASE COUNT         1123 a          662 c          478 g         1015 t
ORIGIN      
        1 aattcaacgc aatttgcaaa tcaagaaaaa attaatcctg ctttgtatga agaagcgttt
       61 acaatttatg gagaatctga agataaatta tattttactt ttaacgcaag tggtcatgca
      121 tgtgtgtatt attttaatgg tagccagtat aaaatataca aagaggtaga atttccaaag
      181 ttcaataaga atagcgaaat caccatcaga agaattgaca atgatatata tttctatatt
      241 gactatttct atctatttag tagataaatc aaaagcctct tctccgtgct gtgtaatatc
      301 aagacctatt tcttcttgct cagcagtaac cctcatcggt aaaattaaat tcgttacttt
      361 atacagcaga tatgaaccac caaaagtaaa tacacccaca attaataatg ctaataaata
      421 aaacataaat tgagtaacat taccatgaat taaccccact ccaaccccat tttccaccgc
      481 aaatacacct gtaagtaaca tacccacaat accgcctaat ccgtggcatg gaaatacatc
      541 taaggtgtca tcaatttttg ttttagattt taattgcaca gctgtataac ttactaatga
      601 ggctacaaaa ccgaagaaaa tactttgccc tacagttaca aaacctgctg cgggtgtaat
      661 agccaccaaa ccaactaccg caccaattgc tgcacctgct gctgaacgtt tcttgcccat
      721 agctccatcc acaaacaacc atgtcatcat agccgttgcc gatgaaatat tagtattaac
      781 aaaagctctt actgccatat catttgcagc taaagaagat ccagcattaa aaccaaacca
      841 accaaaccac aacaatcctg tacctaaaag aatataaggg atgtttgtag gacgagcttc
      901 gctattaacc ccttttcttt tacccaaaaa aattgctcca ctaagtgctg caaaaccagc
      961 agaaatatga actacagtgc caccagcaaa atcaagcaca ccccatttac gtaaaaatcc
     1021 ttctgggtgc caagtccaat gagccaaagg agcatatatt aacagtgaaa aaagtattaa
     1081 aaacaacatc aatgaggtaa atcttaccct ttctgctaat ccaccagtaa taagtgcagg
     1141 ggtaataata gcaaacttaa gttgaaacat agcaaacaat gcaaatggta tagttgggcc
     1201 aaaatcagga tgaggtgtta aacccacgtt cttgaaaaac ataaatgagg taggattgcc
     1261 aataaaaccg cctacacttt cgccaaaagc caaactaaaa ccaacaacaa tccaaagcac
     1321 acttataata cctaatgcaa taaaactttg taataaagta gatactaaat tcttataacg
     1381 caccataccg ccgtaaaaaa aggctaaacc tggtgtcatt aataatacta gtgccgatgc
     1441 catcaataac catgatgtat ctgcatgatt tacttctaat agctctcctc ctaatggaat
     1501 taatgtttcg tggtctacag gtataagaga tacgattgcc acgatagcta aaagtaaaaa
     1561 ggggactctg tacttccatg caactttctt ctgcctcata ataaaacaaa tagtgtgtta
     1621 gttattaata attattgttt tctttttgct agaaaaacga tgcaaattta atcattatat
     1681 tttatttttt aaatatttat atacatttgt attttacaaa aataaaacaa attatatttt
     1741 atacaaaaag ataaaaataa tataacaaat aaataaaata atatgaaaat aaataataat
     1801 ttattagcag acaaatagcg atttaatatt aattagtgat tcttgagtat aaaacaagta
     1861 caaatatttt aatagaatta ggtatctatc agaatatttt ttcccttaaa tagaattata
     1921 caagcacgtg taaactgaaa ataaaaagat aagttgaagc caagcacgaa aagtacttgg
     1981 ctttagtatt tggagaatac tttcgagatt atttaaacaa ctccttttta gcagccaata
     2041 aggtatttcg catcaacgac acaatggtca tgggacctac tccaccaggc actggagtta
     2101 tgtacgaaca ttttggagct acttcatcaa acaatacatc acctgtaagt ttgaagcccg
     2161 atttagttgt agtagatggc acacgggttg ttccaacatc aattaccaca gcaccgtctt
     2221 ttatcatatt tccctttaca aaatttggtt gacccaatgc cgcaattata atatctgctt
     2281 tttggcattg ctccactaaa tcagccgaat agctatgaca taccgtaacc gtagcattgc
     2341 ccggatacgc cttttgaatc attaaatttg ccacaggttt gcctacaata ttacttcttc
     2401 ctagcaccac acaatgtttg ccagcagtag caattccata acgtttaagc aattccatta
     2461 taccagctgg tgtagccgac acaaatgcag gcaatccaat agaagtgcgt cctacattaa
     2521 ttggatgaaa accatctaca tctttgcgat aatcaatggc ttcgataatt ttttgttctg
     2581 aaatatgttt tggcaaaggc aactgtacaa tgaatccatc tatggcagta tcgttattta
     2641 attcactaat ttttgccaac aattcagttt cagtaacggt atcttcgtat cgtatcaggg
     2701 tagaattaaa tccacattgc tcgcaagctt ttactttatg agccacatac gtttcgctac
     2761 caccatcgtg tcctaccaaa atagcagcca aatgtggagg tcgttgaccc atgctcttta
     2821 tagttgctac ttctactgct atttcttgtt taataatatc cgctattgct tttccgtcaa
     2881 taagttgcat agttctttta tttgaatttc gggaatttat taccatttgt ttgtaccata
     2941 cgcatcattt ttcgtatgcc atcaaattgt tttatcaatt tgtttacttc ttgtatagaa
     3001 gtaccactac cattggcaat acgcatttta cgtgtaccat tcagtaattg aggattttct
     3061 ctttcggcag gagtcataga ttgtatcatt gcctcaatgc ctttaaacgc atcatcttta
     3121 atatcaacat ctttcatggc tttgcccata cctggtatca ttgctgccaa atcttttagg
     3181 ttacccatct ttttgatttg ctgaatttga cccataaaat cattgaaatc aaactgattt
     3241 ttggcaattt ttttcgctac cctacgagcc tcttcttc
//
LOCUS       DAOSMY010000168         3261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29505_length_3261_cov_3.129757, whole genome shotgun sequence.
ACCESSION   DAOSMY010000168 DAOSMY010000000
VERSION     DAOSMY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29505_length_3261_cov_3.129757"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..3139)
                     /locus_tag="PLD76_04595"
     CDS             complement(167..3139)
                     /locus_tag="PLD76_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU27314.1"
                     /translation="MDKTLNLQKLYFKDTSFVSLMKERIYNILLIASKYDAFMLEEDG
                     RIDELIFNEYTALNLRYPPRFTIASEHKEVKELLQTRHFDLIIAMPTSDNSHILEMAN
                     ELKVSYADIPIVVLTTLSRRIFKRVFSEGDFLHVDYVFSWLGNSDLLLAIVKLLEDKL
                     NVDDDVASVGVQVILFVEDSIHFYSSILPYLYKHVFTQSLSFMTEALNAHQQMLRMRG
                     RPKILLARTYEEAMFLYKKYQKNMLGVITDVRFPRAGQIHPTAGLELSQEIRKLDPFV
                     PIIVESSEPKGVCEQTDRLCAIFLDKTDKTFHKQFKDAITHHFKFGDLVFVSPDTREA
                     LITVHNLQDLQKHIFDIPANSLFYHLAQNDLSRWLYSRAMFPLAEYLRYIVIKDIKDI
                     DRARHIVFDAIVNYRKIKNKGIVAVFQSDRFDEYSNFARIGEDSMGGKGRGLAFLNAV
                     SKRNPTLYDFENTQITVPKTVVLCTDMFDEFMETNNLYVVALREDVSDEQILEVFLEA
                     RLPRKIMKDLYAFLGVIQSPIAVRSSSLLEDSHYQPFAGVYSTYMVPNVSESRTTTLR
                     MVCDAIKAVYASVFYADSKAYMKATKNLIDEEKMAVVLQEVCGNAYGNRFYPSFSGVA
                     RSLNYYPIGEEKPEEGVVNVALGLGKYIVDGGQTLRFCPAYPNKVLQTSTMEFALSET
                     QNYFYCLDLAQRSFKPRVDDGDNLQKLHIREAEQDGTLRWLASTFDPIDQVIRDGVYA
                     GGRKLITFVNILEHQSFQLADILKNVLTVGEKEMGCPVEIEFAVHLDYETNEQHTFYL
                     LQIRPIVGLNESLTEDISLISKEKTFLSTTNALGNGIMNDVYDVVYVKKGAFNPSKNI
                     LIAYDIEKINKQLVAEDKSYILVGPGRWGSSDHWLGIPIKWPHISGARVIVEAGETSY
                     HIDPSQGTHFFQNLTSFGVCYFTITQNEDDYYDCAYLDEQEALHETEYIRHVRFSSPL
                     LVKVDGRKNRGVVLRGDS"
BASE COUNT         1083 a          652 c          485 g         1041 t
ORIGIN      
        1 cgtagaaaac ctaaaaaaac cgataagtga ggggcgttag ccctgatatc ttagttgata
       61 gttgacaatt gatagttgaa agtataaagt aaacccaaca acaacacccc tcccaaaaaa
      121 actttctaac tttatgaact ttttaacttt ctaactttat gaactttcaa ctatcccctc
      181 tcaaaaccac ccctctgttc tttctcccat caacttttac aagtaatgga gatgaaaatc
      241 gtacatgacg tatatattct gtttcgtgca aagcttcctg ttcgtccaaa taagcacaat
      301 cataatagtc atcttcgttt tgagttatcg taaaataaca cacaccaaaa gacgttaaat
      361 tctgaaaaaa atgagtacct tgacttgggt cgatatgata cgatgtttct ccagcttcaa
      421 caataacgcg tgctcccgaa atatgtggcc acttaatagg aatacctaac caatggtcac
      481 tacttcccca gcgaccagga ccaaccaata tataagattt atcttcagca actaattgct
      541 tatttatttt ttcgatatca taagctatca agatattttt agaaggattg aaagctcctt
      601 ttttaacgta tactacatca tagacatcat tcataatgcc atttcctaaa gcattagtag
      661 tcgagagaaa agttttttct tttgaaataa ggcttatatc ttctgttaac gattcattta
      721 agcctacaat tggacgtatt tgaagtaaat aaaaggtgtg ttgctcattt gtttcataat
      781 ctaaatgtac ggcaaattca atttctactg gacatcccat ttctttttcg ccaaccgtca
      841 atacattttt caatatatcc gccaattgaa aagactgatg ttctaggata tttacaaaag
      901 tgattaactt gcgtccacct gcatatactc catcacgtat tacttggtca ataggatcaa
      961 aagtagatgc taaccaacgt aatgtaccat cttgttcagc ctctctaata tgtagttttt
     1021 gcaaattatc gccatcatct actcttggtt taaacgacct ttgtgctaaa tccaaacaat
     1081 aaaaataatt ttgcgtttca ctaagagcaa actccatggt actcgtttga agcaccttat
     1141 ttggataagc tggacaaaaa cgtaatgttt gccctccatc aacaatgtat ttccctaaac
     1201 ctaatgcaac attaacaaca ccttcttcgg gtttttcttc gccaatagga taataattta
     1261 acgatcgtgc cactcccgaa aaagaaggat aaaaccgatt tccataagca tttccacaca
     1321 cttcttgtaa cactacagcc attttctctt catctatcag gtttttagtg gctttcatgt
     1381 atgctttgct atctgcataa aatacagatg catataccgc tttaatagca tcgcacacca
     1441 tccgtaaagt agtagtacga ctctctgata cattcggaac catataggtg gagtaaactc
     1501 ccgcaaaagg ctgataatga gaatcttcta gtaagctaga cgatcgaact gcaatcggac
     1561 tttgaattac ccctaaaaaa gcatacaaat ctttcatgat ttttcgtggc aatcttgcct
     1621 ccaaaaaaac ctctaaaatt tgttcatcgg atacatcttc tcgcaacgct actacataca
     1681 aattgtttgt ttccataaac tcatcaaaca tatctgtaca aagcacaact gttttaggaa
     1741 cagttatttg cgtattttca aaatcataca aagttggatt acgttttgaa acagcattca
     1801 aaaatgctaa ccctctccct tttcctccca tcgaatcttc tcctattcgt gcaaaattgg
     1861 agtattcatc aaatctatcg ctttgaaaaa cagccacaat acctttgttt ttaatcttgc
     1921 gataatttac aatcgcatca aacacaatat gtcttgccct gtcaatatct tttatgtctt
     1981 taataacgat ataacgcaaa tattcagcta gcggaaacat cgcccgtgaa tagagccatc
     2041 gagataaatc attttgagcc aaatgataaa acaaagaatt tgcaggaata tcaaaaatat
     2101 gtttctgcaa atcttgcaga ttatgtaccg ttatcaaagc ttcacgggtg tcaggagaca
     2161 caaataccaa atcgccaaac ttaaaatgat gcgttatagc gtctttaaat tgtttgtgaa
     2221 aggttttgtc tgttttatct aaaaaaatag cacacagcct gtctgtttgc tcacaaacgc
     2281 cctttggctc acttgactca actataatag gaacaaacgg atccaacttt ctaatttcct
     2341 gacttaactc caatccagcg gttggatgta tctgacccgc tctaggaaaa cgcacatcag
     2401 ttattacacc caacatgttt ttttgatatt ttttatataa aaacatagct tcttcgtaag
     2461 tgcgagctaa taaaatctta ggtcttcctc gcatccgtaa catttgctgg tgagcattca
     2521 gtgcttcggt cataaatgac agcgattgag taaacacatg tttgtacaaa tatggcaaaa
     2581 tagaagaata aaaatgaatg gaatcttcaa caaacaaaat aacttgtaca cctactgagg
     2641 caacatcatc atctacattt aacttatctt ctagcaattt gacaatagcc aataataaat
     2701 cagaatttcc tagccaacta aatacatagt caacatgtaa aaaatcgccc tcagaaaata
     2761 cccgtttaaa gatgcgacgt gataaggttg ttaaaacaac aattggaata tctgcgtaac
     2821 taaccttcaa ttcatttgcc atttccaaga tatgactatt atcacttgtt ggcatcgcaa
     2881 taattaggtc aaaatgacgc gtttgtaata attcttttac ttctttgtgt tcactggcaa
     2941 tagtaaaacg tggtggataa cgcaaattaa gtgctgtata ttcgttgaaa attagttcat
     3001 caatcctgcc atcttcttcg agcataaaag catcatactt gctcgcaata agtagtatat
     3061 tataaatacg ttctttcatt aaactaacaa aagaagtatc tttaaaatag agtttctgta
     3121 gatttaaggt tttgtccatc taatagtttt ttgatgatgc taaggtaatt aaaaaatttg
     3181 agaaagttga atgattagtg gttaatgttt agtggataat tgatagttga tagttgaaag
     3241 tgtttaatgt tgaatgttga a
//
LOCUS       DAOSMY010000169         3258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29546_length_3258_cov_3.205432, whole genome shotgun sequence.
ACCESSION   DAOSMY010000169 DAOSMY010000000
VERSION     DAOSMY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29546_length_3258_cov_3.205432"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLD76_04600"
     CDS             <1..496
                     /locus_tag="PLD76_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOU27315.1"
                     /translation="ASISDSNKKGVLLADVGMPSKLEGRGNMLSEGTQLALDVLSKNK
                     KGFFLLIEGSQIDWAGHANDIDGIIEETLDFDKAVGVAMDFADKNKETLVIVTADHET
                     GGLQILGKDTDTKKLQTIFTSSYHSPVPVPLFAYGKDADKFSSYMENTTIKKILVKLY
                     RFKK"
     gene            complement(538..1365)
                     /locus_tag="PLD76_04605"
     CDS             complement(538..1365)
                     /locus_tag="PLD76_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOU27316.1"
                     /translation="MKQRTYIFPLFILISILFSCTSGERKLVILHTNDVHSAIEPNEK
                     GEGGVAMRCAIIDSIRQSEGDILLVDAGDYFQGTPYFNFFGGAVEIEAMNNMGYDVIT
                     LGNHEFDNGVEALVKQLTSSKATIVSTNYDVSETALKGIVFPYTITEKNGIKVGIFGL
                     GVSPEGLIRNDLFGKIHYKDPINSANKMAFNLKKNEKCDLVVCLSHLGYRYTNNTEKV
                     CDSILAIRSADIDVIIGGHTHDLIVNKKIKNKMGKEVTVAQMGKNGRYVGEIKISIN"
     gene            complement(1370..2119)
                     /locus_tag="PLD76_04610"
     CDS             complement(1370..2119)
                     /locus_tag="PLD76_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOU27317.1"
                     /translation="MSKLYILLVFVLGVGLVTYFVRQPSQQITIHSEAILVNADVGLS
                     PCVAIDEFVQPFKDTIDSEMNTVIGYAPQDMTVGRPQSLLSNFVADLLLERANTIIPT
                     AISVVNMGGLRNSIKKGDVTVRSVFAVMPFENECVIVEMSGEAIWQLANEIVAVKGEG
                     FGGMQVVVKNNTLQQAYVQGKLLDRKASYRVVTSDYLLTGKDGLTALLQHKVLVNPAI
                     KMRDVILEHIVNETKQGKPIIAEMDNRYQIE"
     gene            2340..2687
                     /gene="rplS"
                     /locus_tag="PLD76_04615"
     CDS             2340..2687
                     /gene="rplS"
                     /locus_tag="PLD76_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU27318.1"
                     /translation="MDLLKVAEASFASEKELPAFKSGDTITVSYKIIEGNKERVQQYR
                     GVVIKIVGHGVKKRFTVRKVSENVGVERIFPMDSPFINAIEVNKNGKVRRAKLYYLRA
                     LTGKKARIKERKR"
     gene            2836..3129
                     /locus_tag="PLD76_04620"
     CDS             2836..3129
                     /locus_tag="PLD76_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27319.1"
                     /translation="MTEEHDKLLKSFESRISMLLQKMDSYQSQLATLKDNYDRKNKEL
                     MHAHKTIVELQEKYDNLKIAKTVALSKTDIKDGKLRLSKMMREIDNCIALLND"
BASE COUNT         1172 a          601 c          492 g          993 t
ORIGIN      
        1 agcctctata tccgactcca acaaaaaagg cgtgttatta gcagacgttg gaatgccaag
       61 caagcttgaa ggaagaggaa atatgctttc tgaaggaaca caattagcct tagatgtgct
      121 tagtaaaaat aaaaaaggat ttttcttact tattgaagga tctcagatag actgggcagg
      181 acatgcaaat gatattgatg gaattatcga agaaacgcta gatttcgaca aagctgtagg
      241 tgttgcaatg gattttgcag ataaaaataa agaaacgtta gttattgtaa cagcagacca
      301 cgaaacaggt ggattacaaa tactaggtaa ggatacagat acaaaaaaat tacagactat
      361 atttacaagc agttatcaca gtcccgttcc tgttccttta tttgcttatg gcaaagatgc
      421 tgataaattc tcttcttata tggagaatac aactattaag aaaatactcg taaaattgta
      481 tcgatttaaa aaataaagaa tatacacact aaaaaaagct tctaatcgga gcttttttta
      541 gttaatactg attttaattt cccccacata cctaccattt tttcccattt gtgcaactgt
      601 aacttctttg cccattttat tttttatttt tttatttact atcaaatcat gagtatgtcc
      661 cccaataatt acatcaatat ctgcagaacg tatcgcaaga atagaatcac atactttttc
      721 cgtattgttt gtatacctat atcctaagtg cgataagcac acaaccagat cacacttttc
      781 atttttcttt aagttaaaag ccattttatt agcactatta ataggatctt tataatgtat
      841 tttcccaaat aaatcattgc gtatcaaccc ttctggacta actcccaagc caaatatacc
      901 aactttaata ccattttttt cagtaattgt atacggaaaa acaatccctt ttaaagctgt
      961 ttctgaaaca tcataattag tagatacaat ggtagctttt gatgatgtaa gttgtttgac
     1021 caaagcctca acgccattat caaactcatg gttgccaagc gttatcacat catagcccat
     1081 gttattcata gcttcaattt ctacggctcc tccaaaaaaa ttaaaatagg gagttccctg
     1141 aaaataatcc ccagcatcaa ccaacaaaat atctccttca ctctgcctaa tactatcaat
     1201 aattgcacaa cgcatagcaa caccaccctc gcctttttca tttggctcaa tagcactatg
     1261 tacatcattg gtatgcaaaa tgacgagttt tcgctcgcca gaagtacacg aaaaaagtat
     1321 actgattaat ataaacaggg ggaatatata tgtcctttgt ttcataactt tattcaattt
     1381 gataacgatt atccatttct gcaataatgg gctttccttg ttttgtttca tttacaatgt
     1441 gctccaatat aacatctctc attttaatag caggattcac caatacttta tgttgcaaca
     1501 atgccgttaa cccatccttc cctgttagta aataatcact cgttactaca cgataagaag
     1561 cttttctatc aagaagtttt ccctgaacat acgcttgttg caatgtatta tttttcacaa
     1621 ctacttgcat cccaccaaat ccttcaccct ttaccgctac tatttcgtta gccaattgcc
     1681 aaatagcctc tccactcatt tcaacgataa cacattcatt ttcaaaaggc ataaccgcaa
     1741 acaccgaacg aactgtaaca tcgccctttt ttattgaatt acgcaaacct cccatattga
     1801 caacagaaat agccgtagga ataatggtat tagcacgctc gagcaataaa tcggctacaa
     1861 aattagacaa caaactctgg ggcctgccaa cagtcatatc ttgaggggca tacccaatta
     1921 ccgtattcat ctcactatca atggtatctt taaatggttg cacaaactca tcaatagcaa
     1981 cacacggcga tagccccaca tcagcattta ccaaaatagc ttctgaatga atagtaattt
     2041 gctgtgatgg ttgccttacg aaataagtaa ccaaaccaac gcctagtacg aataccaata
     2101 atatgtataa ttttgacatg ctagttatgt ttcattatat ttcgaaataa aaaagtaagc
     2161 gacaattaca aagtaacgca aaattttgaa taaaaacaaa ttgtgtaaaa taaacatgca
     2221 acagtttgta agataaaaaa ctaatgctat ctttgcagtc gtttttggca taatctcttg
     2281 cacgaacatg ggggtctgta atattatgtc gtaatgttga atttaataaa ttagcaaaaa
     2341 tggatttact taaggtagcc gaagcgtcat ttgcttcaga aaaagaatta cccgctttta
     2401 aaagtggaga cacaattact gtttcttaca aaattatcga aggaaacaaa gaacgtgtgc
     2461 aacaatacag aggtgttgtt ataaaaatag taggacatgg cgtaaaaaaa cgttttactg
     2521 tacgcaaagt atctgaaaac gtaggtgttg aaagaatttt tccaatggac tctcctttta
     2581 tcaatgcaat agaggtaaat aaaaatggta aagttcgtag agcaaaatta tattacctaa
     2641 gagctttaac aggtaaaaaa gcaagaatca aagaacgtaa aagataattt ttttacacat
     2701 aatacacata aaaaggcatc aaccatatat agttgatgcc ttttttagca attagagagt
     2761 cttgtttatg taaaaaatca caaaatattt tttttttcat tcaaaatatt ctatttttgt
     2821 atagatttta caaaaatgac tgaagagcac gataagttac taaaaagttt tgagagtcgt
     2881 atttctatgt tgttgcaaaa aatggattcg tatcaatctc aacttgcaac cctcaaggat
     2941 aattatgacc gtaaaaataa ggaacttatg catgctcaca agacaatagt agaattgcaa
     3001 gaaaaatacg acaacctgaa aattgcaaaa acagttgctc tttctaaaac agacatcaaa
     3061 gatggaaagt tacggttgtc gaaaatgatg cgtgaaattg acaattgcat agcactgctc
     3121 aatgattgat atggaaaaac aagacttact caccattcga gtaatgatat tggacacttc
     3181 ttatcctatc aaaattagaa gagaagaaga agaaatatat cgaaaagcag ctaaacaatt
     3241 gaatgaaaga attttagc
//
LOCUS       DAOSMY010000170         3237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29789_length_3237_cov_3.734444, whole genome shotgun sequence.
ACCESSION   DAOSMY010000170 DAOSMY010000000
VERSION     DAOSMY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29789_length_3237_cov_3.734444"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLD76_04625"
     CDS             <1..197
                     /locus_tag="PLD76_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU27320.1"
                     /translation="KEVGSARTANMVLLGAALPFLSLPEEDIEDAISAIFEKKGEEII
                     KLNIKALRAGTEISRNILNK"
     gene            202..621
                     /locus_tag="PLD76_04630"
     CDS             202..621
                     /locus_tag="PLD76_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOU27321.1"
                     /translation="MLKNILSVTGKPGLYKLLSGNKKMIIAESLIDGKKSPVHSHEKV
                     ISLADIAIYTDTKEVPLREVFATLLEKSEGKESLDSKVSSTDELKKHFATVLPDYDRD
                     RVHNSDIKKIYAWYNLLIKNNITDFSEEKENEEVVSE"
     gene            complement(691..1434)
                     /locus_tag="PLD76_04635"
     CDS             complement(691..1434)
                     /locus_tag="PLD76_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU27322.1"
                     /translation="MISLLKNRATVRQYSDKNISEELLIDLLEVSFRSSNTGNMQVYS
                     VVVTRDEEMKKQLAPCHFNQPMIVQAPIVLTFCADFNRFTKWCEYNKAIPGYDNFLSF
                     ITATIDATIVAQTFAIAAESKGLGICYLGTTNYFAEKISSILHLPRFVVPITTITVGY
                     PASIPNQTDRLPTRSLVHYEQYTDYTEDAIRNMYAEKENLPEMKQFVLDNKKETLAQV
                     FTDVRYTKSNNETFSAMLLSFLQKQGFRV"
     gene            1756..>3237
                     /locus_tag="PLD76_04640"
     CDS             1756..>3237
                     /locus_tag="PLD76_04640"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOU27323.1"
                     /translation="MEQKTYTFDEALQASIPYFEGDELAAKVWINKYALKDSYGNIYE
                     KTPDDMHWRLANEIARIESKYPNALTADELFELMRNFKYIVPQGSPMTGIGNHHQIAS
                     LSNCFVIGFTGESDSYGAVIKIDEEQVQLMKRRGGVGHDLSHIRPNGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLSVSIKHPDAESFIDAKLEQGKVTGANVSVKI
                     HDDFMNAIINKVPYVQQYPVFSKEPKFKKEIDAEQLWKKIVHNAWKSAEPGVLFWDTI
                     IKESLPDCYADLGYQTVSTNPCGEIPLCSYDSCRLLALNLYSYVENPFTSEAKFNFEL
                     FKKHSQLAQKIMDDIIDLEMEKIDKILEKIASDPEDASIKLTEEQLWLKIKNKTEKGR
                     RTGVGTTAEGDMLAALGLRYGSEKATEFSELVHKTLAIEAYRSSVTMAKERGAFEVYD
                     TEREKNNPFVLRLKDADPELYSEMAKYGRRNIACLTIAPTGTTS"
BASE COUNT         1200 a          491 c          557 g          989 t
ORIGIN      
        1 caaaagaagt aggttctgca cgcactgcaa atatggtttt attaggagct gctctcccat
       61 ttttatcttt accagaagaa gatatagaag atgctatatc tgccattttt gagaaaaaag
      121 gagaagaaat tattaaatta aacatcaaag ccttacgtgc tggaactgaa ataagtagaa
      181 acattttaaa taaataataa tatgctgaaa aatattttat ctgttactgg aaaacctggt
      241 ttatataaat tattgtctgg aaataaaaaa atgattatag ccgaatcatt aattgatggt
      301 aaaaaatcgc ctgtgcactc gcacgaaaaa gtaatttcgt tggcagatat tgccatttat
      361 actgatacta aagaagttcc cctgcgtgaa gtgtttgcta cgttgcttga aaaaagtgaa
      421 gggaaagaaa gccttgattc aaaggtatct agcaccgatg aactaaaaaa acactttgca
      481 acagttttac ctgattatga tagagataga gtgcataata gtgatatcaa aaaaatatat
      541 gcctggtata acctactaat caaaaataat attacagatt tctcggaaga aaaggaaaac
      601 gaagaagttg tatctgaata aatatgtttc atcgtttagg ttatagctaa ggagcaaaga
      661 cgtaaacgat gaaaattaaa acagattgtg ttatacccta aaaccttgtt tttgaaggaa
      721 actcaataac attgcagaaa atgtttcgtt attacttttt gtataacgaa catccgtaaa
      781 tacttgagcc agtgtttctt ttttgttatc taaaacaaat tgtttcattt caggcaggtt
      841 ttctttttct gcatacatgt ttctaatagc atcttctgta taatcagtat actgttcgta
      901 atgcaccaat gatctcgttg gtaatctatc tgtttggttt ggtatagaag ctggataacc
      961 tactgtaata gttgtaatag gcacaacaaa acgaggtaaa tgtaatatgc tgctaatttt
     1021 ttcggcaaaa taattggtag tacctaagta acatatgccc aagccttttg actctgcagc
     1081 aatagcaaag gtttgagcaa caatggtagc atcaattgtg gctgtaataa atgacaagaa
     1141 attatcatac cctggaatgg ctttgttgta ttcacaccat ttagtaaacc ggttaaaatc
     1201 ggcacaaaaa gttaaaacta tcggagcttg tacaatcatg ggttgattaa aatgacatgg
     1261 agctaattgt tttttcattt cttcatcgcg tgttacaacc acactatata cttgcatatt
     1321 gcccgtattt gaacttctaa atgaaacttc taataaatct attaacaatt cctcacttat
     1381 gtttttatcc gaatactgac gaacagtagc tctattttta agtaaactaa tcatatggta
     1441 tatatgtatt tattaattta caaaaatact caatttctct caataaacaa attaaataaa
     1501 ttacgaatat taaattaatg tgattttttt ttgacaactt tttcatctaa aaatattaat
     1561 aaaatactaa taatcatgta tttataaaat atttttgaca actttacaaa aaagaaaaat
     1621 aaatttactg caattatttt acatttttat ttataaaaaa ctttgttaag taaaaaaaat
     1681 agtgtaacat tgtagtcccg tgaagcacga aaccaacttt ttataatcga catacaatca
     1741 atattttaga aaaaaatgga acaaaaaacg tacacatttg atgaggcatt gcaagcatca
     1801 atcccttatt ttgaaggaga tgagttagca gcaaaagtat ggataaacaa atacgcacta
     1861 aaagattctt acgggaatat ttacgaaaaa acacctgatg atatgcattg gcgtttggca
     1921 aatgaaatag cacgtattga atcaaaatat ccaaatgcat taacagctga tgaattgttt
     1981 gaattaatgc gaaatttcaa gtacattgtt ccacaaggaa gccccatgac gggtattggc
     2041 aatcatcacc aaatagcatc cttatccaat tgttttgtta ttggttttac tggcgagtcc
     2101 gattcgtatg gagctgtaat aaaaatagat gaagaacaag tgcaattgat gaaacgtaga
     2161 ggtggcgttg ggcatgattt gtctcatatt cgcccaaatg gttctccagt aaaaaattca
     2221 gcacttacat caacaggtat tgttccgttt atggaacgct attcaaactc tacacgcgaa
     2281 gtagcccaag acggaagaag aggagcatta atgttaagtg tatcaattaa acaccccgat
     2341 gcagagtcat ttattgatgc aaagctagag caaggaaaag taacaggagc gaatgtatca
     2401 gtaaaaatac atgatgactt tatgaatgct atcatcaata aagttccata tgttcaacaa
     2461 tatccagtat tctcgaaaga acccaaattt aaaaaagaaa tagatgctga acaattatgg
     2521 aaaaagattg ttcacaacgc atggaaatct gccgaaccag gtgttttatt ttgggataca
     2581 atcataaaag aatctttacc agattgttat gccgatttag gttatcaaac ggtatctact
     2641 aacccttgcg gtgaaattcc tttatgttcg tatgatagtt gtcgtttact ggcattgaac
     2701 ttatattctt atgtggaaaa tccatttact tctgaagcca aattcaattt tgaattattc
     2761 aaaaaacatt ctcaattagc acaaaaaata atggatgaca ttattgattt ggaaatggaa
     2821 aaaattgata aaattctaga aaaaattgct tctgacccag aagatgctag cattaaatta
     2881 acagaagaac aactgtggct aaaaattaaa aacaaaacag aaaaaggacg tagaacgggt
     2941 gttgggacaa ctgcagaagg agatatgttg gcagctttag gattacgcta cggaagtgaa
     3001 aaagcaacag aattttccga attagtacat aaaacattag ctatagaagc atatcgttcg
     3061 tctgtaacaa tggctaagga aagaggggct tttgaggtat atgatacaga aagagaaaaa
     3121 aacaatccat ttgtattgcg gttaaaagat gctgatcctg aattatacag tgaaatggca
     3181 aaatatggac gcagaaacat cgcatgttta accattgctc ctactggaac cacaagt
//
LOCUS       DAOSMY010000171         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29841_length_3232_cov_3.973875, whole genome shotgun sequence.
ACCESSION   DAOSMY010000171 DAOSMY010000000
VERSION     DAOSMY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29841_length_3232_cov_3.973875"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PLD76_04645"
     CDS             <1..1189
                     /locus_tag="PLD76_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU27324.1"
                     /translation="QEQARNQSSVYEILNGVTEMKLNQFEEFKRKEWERIQEKLFKIN
                     IRILRIDQFQNSGFEFFNQLKNILVTFLAAYYVVQGQMTLGALMSVSYIIGQMNSPVN
                     QLVNFFRSLQDARLSVERLNEVQYLLEEEQPDQIYYSPDNTNVHEQMTKGIHLKNISF
                     QYQGPMSPYVLKGIDLNIPEGKITAIVGTSGSGKTTLMKLLLKFYEPVNGNIFYNSHN
                     VKNLSAKSLRQNVGVVMQDGYIFSDTIERNIATGDEEIDSERLQQAVEIAHIKDFIEE
                     LPLGLKTKIVASGNGISGGQRQRILIARAVYRNPHYIFFDEATSSLDAKIEKYIHDSL
                     KHFFVGKTVIIIAHRLSTVKSADQIVVLEHGKIIEIGNHEELTHKKGAYYDLVKNQLE
                     LGN"
     gene            complement(1229..2221)
                     /locus_tag="PLD76_04650"
     CDS             complement(1229..2221)
                     /locus_tag="PLD76_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOU27325.1"
                     /translation="MNLYRLFCGKLTIKRLINYILLRLEYFISYSSKTPFIHAKPFAA
                     SIEPANYCNLQCPECPTGMGAIQKEATTLPFPLFKKMINPLLPELFYLNLYFQGEPFL
                     NESIYDMIAYANRQKIITNISTNGHFLTDSACQQLITSKLDRIIISLDGATEETYEKY
                     RVGGNFTAVVDGITRLVRLKKEMQSRRPFIELQFIVFSTNEHEIPAIKVLAKSLQVDG
                     LSIKTAQLTNFKKGNPLMPKNTIYSRYKLDKNGSYKRKKPLRNRCWKNWSSIVVSTDG
                     KVLPCCFDKHGLYAYGNLLDNGFHSLWNGDKATKFRSSILHNRKSLPMCNNCTE"
     gene            2447..>3232
                     /locus_tag="PLD76_04655"
     CDS             2447..>3232
                     /locus_tag="PLD76_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27326.1"
                     /translation="MKFTKTLFRFGAVVLLASVFASCSKMGGLPAEYITVNPNPLEVK
                     ANKVEAEITGTFPEKYFAKKAILEVTPVLRYEGGEAVGTTVIYQGEKVKDNNKVVAYK
                     TGGKFTQNISFDYVPGMKKSKLFLTFKAKIGKKEIQIPEVEIAQGTIVTATLANPLEI
                     AGATGADAFQRVIQEKYEAAILFQIQRAELQKKALTTAEVAALQKQLTETQKADNKQI
                     ASLGIASFASPDGPTDLNEKLAANRKKNTEGFLTKELKKSKIDA"
BASE COUNT         1142 a          527 c          565 g          998 t
ORIGIN      
        1 tcaagagcaa gcccgaaatc aaagctcagt ttacgaaatt ttaaacggag ttacagaaat
       61 gaaactaaat cagtttgaag aatttaaacg aaaagaatgg gaaagaatac aagaaaaatt
      121 attcaaaatt aatatacgta ttttacgcat tgaccaattt caaaattctg gttttgagtt
      181 ttttaatcaa cttaaaaaca tattagttac ttttttagct gcctactatg tagtacaagg
      241 tcaaatgact cttggtgcat taatgagtgt ttcgtatatt attggtcaga tgaactctcc
      301 tgtaaatcaa ttagttaatt tttttcggtc gctacaagat gcccgtttat ctgtagaacg
      361 actcaacgag gtgcaatatc ttcttgaaga agaacaaccc gatcaaatat attattcgcc
      421 tgataatacg aatgtacatg aacaaatgac taaaggtatt catttgaaaa atatttcgtt
      481 tcagtaccaa ggaccgatgt cgccttatgt gctaaaaggc attgacctaa acatacccga
      541 agggaaaata actgccattg taggcaccag tggcagtggc aaaacaacat tgatgaaact
      601 actgctaaaa ttttatgagc cagtaaatgg aaatatattt tataatagtc ataatgtcaa
      661 aaatttatca gcaaagagct tacgtcaaaa tgtaggggtc gtaatgcaag atggctatat
      721 tttttcagac actattgagc gtaacatagc tacaggtgat gaggaaattg atagtgaaag
      781 attgcaacaa gctgttgaga tagcacatat taaagatttt attgaggaac tcccgctcgg
      841 acttaaaacc aaaattgtag catcgggtaa tggaatttcg ggaggtcaga ggcaacgaat
      901 tttgatagca cgtgcagtat atcgaaatcc tcactacata ttttttgatg aggctaccag
      961 ctcgctcgat gctaaaattg aaaaatatat tcacgactca ttaaaacatt tttttgttgg
     1021 taaaactgta atcattatag cacaccgact ttcgacggtt aaaagtgctg atcaaattgt
     1081 tgttcttgaa catggtaaaa taattgaaat aggtaaccac gaagaactaa cccacaaaaa
     1141 aggagcatat tatgatttag taaaaaacca acttgaattg ggcaattaaa catccattta
     1201 atcgaataat gcttctaaat acttttgttt attctgtgca attgttgcac ataggcaaag
     1261 attttctgtt atgcaaaatg cttgagcgaa actttgttgc cttatcgcca ttccacaagg
     1321 agtgaaaccc attatctaat aagttgccat aagcatatag cccgtgttta tcaaaacaac
     1381 aaggaagcac ttttccgtct gttgaaacta cgatagaact ccaatttttc caacatcgat
     1441 tacgcaaagg ctttttccgt ttataggagc cgtttttgtc cagtttatat ctactgtata
     1501 tagtattctt tggcattagt ggattaccct ttttaaaatt agttaattga gccgtcttaa
     1561 tagataagcc atctacctgt agtgattttg ctaatacctt aattgctggt atttcgtgtt
     1621 cattcgtact aaaaacaata aactgaagtt cgatgaaagg tcttcttgat tgcatttctt
     1681 tttttaaacg cactaggcgt gtaattccat ccacaaccgc cgtaaaatta ccacccaccc
     1741 tgtatttttc gtatgtttct tctgttgctc catcaaggct aataataatt ctatcaagtt
     1801 tagatgttat gagttgttgg catgctgaat ctgttaaaaa atgcccattg gtagatatat
     1861 tggtaatgat tttttgccta tttgcatagg caatcatgtc ataaatactt tcattcaaaa
     1921 aaggctctcc ttgaaaatat agattgaggt aaaatagttc aggaagaaga ggattaatca
     1981 tctttttaaa caaaggaaac ggtagagtag tagcttcttt ttgtattgct cccatacctg
     2041 tagggcattc aggacattgt aaattgcagt aatttgctgg ttctatagat gcggcaaaag
     2101 gttttgcatg tataaaaggc gttttactac tatacgagat aaaatattct aaacgtaaaa
     2161 gaatatagtt aattagacgt tttatcgtaa gcttaccaca aaaaagccga tataaattca
     2221 tatcccttat tatttacagc aaaagtcagc attattatgt agaacagcaa agattttgta
     2281 ttaaatatct ttgagatgag gttaaaaata ccaaaaatag aattagaaag actccattga
     2341 gttttacaac aaaattcttt aaacaaaaag ttggtattat tttttatatt atgtatcttt
     2401 gccctaattc tgtaaaatga atgtataacc caattaattg ataagtatga aattcacaaa
     2461 aacattattt cgttttggag ctgtagtatt actggcttcc gtatttgcat catgtagtaa
     2521 aatgggcgga ttacccgctg agtatatcac agttaacccc aatcccctcg aagtaaaagc
     2581 aaacaaagta gaggcagaaa ttacaggaac ttttcctgaa aaatattttg ccaaaaaagc
     2641 tattttggaa gttacaccag tattgcgtta cgaaggtggt gaagctgtag gtacaacagt
     2701 aatttaccaa ggagaaaaag taaaagataa caacaaagtt gtggcataca aaacaggtgg
     2761 aaagtttact caaaacattt cgtttgatta tgtgccagga atgaaaaaat caaaattatt
     2821 tttgactttc aaagcaaaaa taggtaaaaa agaaattcaa attccagaag ttgagattgc
     2881 acaaggtacc attgtaacag ctacattagc aaatccatta gaaattgctg gagctacagg
     2941 tgctgatgca ttccaacgtg ttattcaaga aaaatacgaa gctgctattt tattccaaat
     3001 tcaacgtgct gaattgcaaa aaaaggcact tactacagca gaagttgctg ctttgcaaaa
     3061 gcaacttaca gaaacacaaa aagctgacaa caaacaaatc gcttctttag gtatcgcttc
     3121 ttttgcttcg ccagatggtc caactgactt gaacgaaaaa ttggctgcta atcgtaaaaa
     3181 gaatactgaa gggttcttaa caaaagagtt gaaaaaatca aaaatagacg ct
//
LOCUS       DAOSMY010000172         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_29924_length_3224_cov_3.000316, whole genome shotgun sequence.
ACCESSION   DAOSMY010000172 DAOSMY010000000
VERSION     DAOSMY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29924_length_3224_cov_3.000316"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..618)
                     /locus_tag="PLD76_04660"
                     /pseudo
     CDS             complement(43..618)
                     /locus_tag="PLD76_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
     gene            complement(647..2002)
                     /locus_tag="PLD76_04665"
     CDS             complement(647..2002)
                     /locus_tag="PLD76_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOU27327.1"
                     /translation="MAKVIHLKKGLDIKLKGAPLEIIGKATQSELIGFVPEHFAGIIP
                     KPVAKPGDKLKVGSVLFVDKNCPELRIVSPVSGELVAINRGERRKILDIVIKNDFKME
                     QEIFPTIDWKNASDKEVKQMLLSLGLFAFIKQRPYDIVANPNDQPKAIFISTFDTAPL
                     APDYTFILKDQVADFQAGITALSTLAPVSVGIKAGNGIFDGLKNATLHYFKGAHPAGN
                     VGVQINNVNPVNKGEVVWTINPQEVLFIGRYVNKGTLDFSKLVASTGSEVTTPAYYPS
                     IVGSPITPIVKANVSQGISLRYISGNVLSGEKITENGFLNPFHSQISVIPEGDDNHEL
                     FGWIMPRWDKFSNSGTYFTKLVQFLNPKKTFEADARLLGGERHMIMSGEYDSVLPMDI
                     YPEFLIKAIIANDIDKMEALGIYEIAPEDVALCEFVCSSKVPLQKIVREGLDMLRKEM
                     N"
     gene            2169..>3224
                     /locus_tag="PLD76_04670"
     CDS             2169..>3224
                     /locus_tag="PLD76_04670"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOU27328.1"
                     /translation="MKKGNYVKLIVSILLFPMCSLMANVYHTQIFDTDIHTLRVYNPN
                     QKPYYPVVDLHVNEYVELSFDDLHPSFRLFSYKIIHCNADWTVSNATEIEYAEGFSTG
                     NIEDSSPSINTYVPYTHHSIRFPNENVRFKQSGNYAIVIYTNNDEQQVALTARVYVSE
                     NSITINGTVSGITDIDYKKEHQQLSIDIIPNNFTIHNPYRDIKVIVQQNQRMDNEVSN
                     VVPSIVQGNKISYINERKLIFAAGNEFRNFDLSATRILSRRIEDISFVQTQYHALLYP
                     DEIRKKAWYTQDYDINGRIIVNIQGTTENDTEADYFFVHFSLPSTLLPEDVYLLGQFN
                     HYHMDSSSIMKYNYEKRC"
BASE COUNT         1085 a          631 c          512 g          996 t
ORIGIN      
        1 tacctcatct cctgacattt tggtaggata cgcaaagaac taaaatgcac gtgccaccaa
       61 tgctacgttg aaaatattca ttcctgttcc tccaaacacc tcttttgcaa aaattacgga
      121 gaatgcagta gcaacggcta acatccaaag aggtaaatca acaggaacga taagaggaat
      181 aagtattccg gacactaaaa atccttcatg tatttcttct tttttccatt gagctacagt
      241 aaattcaata cccaaaccaa caacatacga aactataact ttgggtaata ccaatgctac
      301 tccatacaaa aagatgttcc aaaattgagc tgtttgtgga ttccaatatg tagcaacgag
      361 ctgccccgtg gctaaaaaat gttggtaacc aacattaaac atcccaaaca gtaaagcagg
      421 aactaatgct ataacaacca tcgacatcat acgtttagag tcaacagcat cgtgaatatg
      481 cgtgccagat tttgatgttt tgcttggcac aaacaaaaag gtttcaaacc catcaaaaac
      541 ggaatggaac atcgataatt taccaccttc ttggaagttg ggtttaattt tatcgactaa
      601 ttttctaagt gctttcaatg tattaggtat ttaaatatat taataatcaa ttcatttcct
      661 ttcgcaacat atccaatcct tctctcacaa ttttttgtaa gggaacttta gacgagcaaa
      721 caaattcgca taatgcaaca tcttctggtg ctatttcgta aatccccaag gcttccatct
      781 tatctatatc atttgcaatg atggctttaa tcaagaattc aggatatata tccattggca
      841 atacactatc atactctcca ctcattatca tatgtcgttc gcctcctaac aatcgtgcat
      901 ctgcttcgaa tgtttttttc gggtttaaaa attgcactag tttagtaaaa tatgtaccac
      961 tattactaaa tttatcccaa cgtggcatta tccaaccgaa caattcgtga ttatcatctc
     1021 cttcaggtat aactgagatt tgagaatgaa aaggatttaa aaagccattt tcagtaattt
     1081 tttcaccaga aagcacatta ccactaatat agcgtaaaga aataccttgg cttacgtttg
     1141 cttttacaat cggtgttatt ggagaaccaa caattgatgg gtaatacgct ggcgttgtaa
     1201 cttcagaacc agtagatgct accaattttg agaaatctaa ggtaccttta tttacatatc
     1261 gaccaataaa caatacctct tgaggattga tagtccaaac aacctctcct ttgtttactg
     1321 gattcacatt attgatttga acacctacat taccagcagg atgagctccc ttaaaataat
     1381 gtaaggttgc atttttcaaa ccatcaaata tcccattgcc tgctttaatc cctacagaga
     1441 caggtgctaa ggtagacaag gctgtaatac ctgcttgaaa atcggctact tgatctttta
     1501 aaataaacgt gtaatcggga gccaatggag cggtatcgaa agtagagata aaaatggctt
     1561 tgggttgatc atttggatta gccacgatat catatggacg ttgcttgata aaagcaaata
     1621 atccgagtga taaaagcatt tgctttactt ctttgtctga agcatttttc caatcaatag
     1681 taggaaaaat ttcttgctcc atcttgaaat cgtttttgat aacaatatcc aaaattttac
     1741 gacgttctcc tctgtttata gctactaatt cgccacttac tggagaaact attcgtaact
     1801 ctggacaatt tttatccaca aataatactg aacctacttt cagtttgtca ccaggtttag
     1861 ctacgggttt gggaataatt cctgcaaaat gttcaggaac aaaaccaatc aactccgatt
     1921 gtgtcgcttt tcctattatc tcaagaggag cacctttgag tttaatatct aagccctttt
     1981 ttagatgtat tactttagcc ataaaaatga ggttgaatac tgtttttgta aaaacttcgc
     2041 aaatttagaa aaaataacga tatgtacaag ataaaagttc cctttttaac actttatttt
     2101 ttacagataa taaatagtat caaaaagaca gtttgcagtt tcatttttta attatatctt
     2161 tgccaacaat gaaaaaggga aactacgtaa aattgatagt gagcatattg ctttttccga
     2221 tgtgctctct tatggcgaat gtttatcaca cccagatttt tgatactgat attcacacat
     2281 tacgcgtata caatcctaac caaaaacctt actacccggt tgttgattta catgtaaatg
     2341 aatacgttga gctttcgttt gacgatttgc acccatcgtt tcgtcttttt tcgtataaaa
     2401 ttatacattg caatgctgat tggacagtat cgaatgccac agaaatagaa tacgccgaag
     2461 gtttttctac tggaaacata gaagattcga gtccatcaat caacacttat gtgccttata
     2521 cacaccactc aatacgtttc ccaaacgaga atgtacgatt taaacaatcg ggaaattatg
     2581 ctatcgtaat ttatactaac aacgatgagc aacaagtagc actaacagca cgtgtgtatg
     2641 tatcggaaaa tagtattaca atcaatggaa cagtgtctgg aatcactgat attgattata
     2701 aaaaagagca tcaacaactc agtatagata ttataccgaa caattttact atccataatc
     2761 cttaccgcga tataaaggta attgtacaac aaaaccaacg aatggacaac gaagtaagta
     2821 atgtagtacc cagtatcgtt caaggcaaca aaattagcta tatcaacgaa cgtaaactca
     2881 tttttgcagc aggcaacgaa tttcgtaatt ttgatttgtc tgccacacgg attttaagtc
     2941 gtcgaataga ggatatttct tttgtacaaa cacagtatca tgcattactc taccccgacg
     3001 aaatacgcaa aaaagcgtgg tacacacaag attacgacat aaacggtaga ataattgtga
     3061 atatacaagg tacaactgaa aatgacaccg aagcagatta tttttttgtt catttttcgt
     3121 tgccctctac actgcttccc gaagacgtat acctactggg acagttcaat cattatcaca
     3181 tggacagctc ttctataatg aagtataatt acgaaaaaag atgc
//
LOCUS       DAOSMY010000173         3215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_30018_length_3215_cov_4.173734, whole genome shotgun sequence.
ACCESSION   DAOSMY010000173 DAOSMY010000000
VERSION     DAOSMY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30018_length_3215_cov_4.173734"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..2672)
                     /locus_tag="PLD76_04675"
     CDS             complement(93..2672)
                     /locus_tag="PLD76_04675"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU27329.1"
                     /translation="MKNWISQPFVPHIVAVVVFFLLTFFYLTPAFQGNIIFGHDTLSY
                     VASAKEAHTYNEQNDDQALWTNSMFGGMPTYQISMTQPQSILTYIDAVLRVLPGPSYR
                     LFLYLVGFYVLLVAFRVNPWLSIGGAIAFALGSYNLIIIIAGHNTKAVAIAYMAPIIG
                     SVYMAFREKRLLGTVLLTLFLGLSLFANHIQITYYTLFIVLCFGVSEFIFSVKEKKLV
                     PFFTTVGILLVGVVIAVGLNATRLLTTAEYAAYTMRGASNGLSLDNGQEQKGLSKDYI
                     TQWSYGIDETLTLFIPNVKGGSSVGKLTEDSQTAMKFAELSGYKPLGRNLTKEEQSRI
                     DSILKRNPDNFTPKNLAELMYMYELPLYWGTQPFTSGPVYIGAIVIFLFFAGLYLLPA
                     RDKWWMLAATLLGIMLAWGKNFMPLTDFFIDYVPMYNKFRTVSMTLTIPAITLTLMAI
                     LTLKSFFNTNVSIDSKRKALYIGGGIAGGITFLFWLFPSLAGSGVSPQDVNYTGNFAF
                     LQQTLPQDRLQLVSQDAFRSLLFIVLATLVIWLSIEGKIKQYIAYPAIIILFTIDMLP
                     IASRYLNNASFVPDTITTTSFPKREVDKIILQDKDPNYRVLDVTVDIFNSAVPSYYHK
                     TIGGYHAAKMRRYQEMIEVHLAKQIQAITASFATAKTENDIQATMQQQQVLNMLNMKY
                     LIYHADAPPIQNRYAFGNAWVVKNSMPVASANEEMLALTTVDLASTVVIDTTTQTFFS
                     SVPLSSDSLARVELSLYSPNELRYTFSSTSNQMVVFSEIFYDKGWNAYIDGEKTPYYR
                     ANYILRAMNVPLGTHEIVFRFEPKSYEIGNILSLISSILLTFAFLGIIFAGSKKNKKN
                     RQA"
BASE COUNT         1060 a          593 c          585 g          977 t
ORIGIN      
        1 ctcgggcatt aaccaaatga cggagggcgg gttttgaaga aagatctttt ctcataattg
       61 tatggtaagg taaaataagc gtatctaatt ttttatgctt gtctattctt tttattcttt
      121 tttgaacccg caaagataat acctaaaaaa gcgaaagtca atagtataga actaattaaa
      181 cttaaaatat taccaatttc gtacgattta ggttcaaaac gaaaaactat ttcgtgtgtg
      241 cccaaaggaa cattcatagc tctaagtata taattagctc tataatacgg ggtcttttcc
      301 ccatcaatat aggcattcca tcctttatcg taaaaaattt cagaaaatac caccatctga
      361 ttacttgtgg atgagaaagt ataccgcaat tcattcggtg aatacaagga taattcaaca
      421 cgagcaagcg aatcggaaga taaaggaaca gaggaaaaga aagtttgtgt tgtggtatca
      481 ataacaactg ttgaagctaa atcaactgta gtgagtgcaa gcatctcttc gttggcagaa
      541 gctactggca tactattttt tacaacccaa gcatttccaa aagcatatct gttttgaatt
      601 ggaggagcat cggcatgata tatcaagtat ttcatattca acatattcaa tacttgttgt
      661 tgttgcatcg ttgcttgtat atcattttcc gttttggcag tagcaaaaga agctgtaata
      721 gcctgaattt gttttgcaag atgcacttca atcatttctt gatatctacg cattttggca
      781 gcatgatacc cacctattgt cttatgataa tacgaaggaa ctgcactatt aaaaatatca
      841 accgttacat caagaacacg gtaattagga tccttatctt gcaaaataat tttatctacc
      901 tccctcttag gaaatgatgt ggtagtaatc gtgtctggca caaaactagc attatttaaa
      961 taacgacttg ctatgggcag catatctata gtaaataata tgatgatagc tggataggct
     1021 atatactgtt tgatttttcc ctctatggat agccatataa caagagttgc taacacaata
     1081 aataacagag agcgaaaagc atcttggctt accaactgca accgatcttg tggtaaggtt
     1141 tgttgcaaga atgcaaaatt gcccgtataa ttaacatctt gaggggatac acccgagcct
     1201 gcgagagaag ggaatagcca aaataaaaaa gtaattcccc ctgctatacc tccaccaata
     1261 tacaaagctt tacgttttga atcaatagat acattggtat taaaaaacga tttcaacgtt
     1321 aatatagcca tcaatgtcag ggtaatagca ggaatggtta atgtcatcga aactgttctg
     1381 aacttattgt acattggcac ataatcaata aagaaatcag ttagcggcat aaaattcttt
     1441 ccccacgcca acataatgcc tagtaaagta gcggctaaca tccaccactt gtcgcgtgca
     1501 ggcaataggt acaaccccgc aaaaaataag aaaattacaa tggctccgat atacactgga
     1561 cccgatgtaa aaggttgtgt tccccaatac aggggtaact cgtacatata catcaactct
     1621 gctaagtttt tgggcgtaaa attatctgga ttacgtttca aaatgctatc tatacgactt
     1681 tgctcttcct tggttaagtt tcgacccaaa ggtttatacc ccgacaactc tgcaaatttc
     1741 atagctgttt gggaatcttc tgttaactta cccacagatg agccaccttt cacatttggg
     1801 ataaacagcg taagcgtttc gtctatacca tagctccatt gggtaatata gtctttgctc
     1861 aatccctttt gctcctgtcc attatctaac gataaaccgt tagaagctcc acgcatggta
     1921 taagcagcat attcggctgt tgtaagcaaa cgtgtggcat ttaagccaac cgcaataacg
     1981 acacccacca acaagatgcc aacagtagta aaaaaaggaa caagtttttt ctcttttaca
     2041 ctaaaaataa attcggacac cccaaaacac aacacaataa acagggtata ataggtaatt
     2101 tgtatgtggt tagcaaaaag cgataagcct aaaaacaagg tcagcaatac ggtaccgagc
     2161 agccgtttct ctctaaaagc catatacaca ctgccaataa taggtgccat ataggcaatg
     2221 gcaaccgctt tggtattgtg acctgcaata ataatgatta aattgtacga ccccaaagca
     2281 aacgcaatag cacctcctat gctcaaccat ggattgacac gaaacgccac caacaacaca
     2341 taaaaaccaa ctaaatacaa aaacaagcga taactaggac ctggcaacac cctgagtaca
     2401 gcatcgatat aggttaatat actttgaggt tgcgtcatcg aaatttgata agtaggcata
     2461 ccaccaaaca tggaatttgt ccatagggct tggtcgtcgt tttgctcgtt gtaggtatga
     2521 gcctctttgg ctgaagctac atagctaagc gtatcgtgac caaaaatgat atttccttga
     2581 aatgcgggag ttaaataaaa aaaagtaagt agaaaaaata ctacaacagc cactatatgt
     2641 ggaacaaatg gttgagaaat ccaatttttc atttttttgg gtataagggt ccagttatta
     2701 gcagcaaata ctatgctttt tgtaaaacac cacaaagtaa gtattttttg ttgaaaaaat
     2761 gaaataatat gcaagaaagg atgttttttt gctaggtgtt gtgttgggaa ttagtgagta
     2821 tgtgtatttg tttggaattt aatttgttta ttttgatgag tggatttgca tcaacttgtt
     2881 gttacaaaca atacaccaaa aactcctcgc tgaaaaggag gaggtgttag tgtgaaaaaa
     2941 tgtatgttca gcggattgga aaatccttat aagatagttc aggttcgatt tagcaatctg
     3001 acctgacgga agagctttgt ccttgtaaac tttatttttt gtgtttgtct gtttaatgat
     3061 tcttccattg ttatctgttt ttagtgttgc ttgtaacgtt cctggtatag ccacagtttt
     3121 ttattctatc cagttaaatt gtttataaat atactgttta ttcctactct ttaacattcc
     3181 tatcaagttg gtaatatgtg tctgtaaact tgtta
//
LOCUS       DAOSMY010000174         3213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_30046_length_3213_cov_3.493667, whole genome shotgun sequence.
ACCESSION   DAOSMY010000174 DAOSMY010000000
VERSION     DAOSMY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30046_length_3213_cov_3.493667"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..413)
                     /locus_tag="PLD76_04680"
     CDS             complement(153..413)
                     /locus_tag="PLD76_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU27330.1"
                     /translation="MVKVRSKTVEPVIGTLVNFTNMKRVNTRGIKNANKHVLMASLTY
                     NLKKYLRFITKKPSILAQVLSLKQGKNFAFVNQSFKTSITPL"
     gene            complement(407..1504)
                     /locus_tag="PLD76_04685"
     CDS             complement(407..1504)
                     /locus_tag="PLD76_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU27331.1"
                     /translation="MLLVGYLNNINSDRQLIAFCSDSLSIRLFLGYDVHEQLPWHSTI
                     SRTRGLYGEEVFLNLFKEVLKMCVSKGMVRGKRQAVDSVFIKANASMDSLVEKEVLED
                     AGAFVNEFEENSEYKVTSTRKKFVERHHDWKAEAYKGMPANSNSNQIDENGNLIRPKY
                     LSNHTHYSPTDSDARVSVKPGKARQLNYFGQIAVDDAHHVITGACSDFADKRDSQCLE
                     QIVELTEKNLNENGIELEELLADGGYSSGEALAYLHEKNINAYIPNFGQYKSEREGFT
                     YHKEENYYQCTKPEGKQAKLLFKGEKMDSKGYTKRTYRSSETDCKNCPLREQCCGKST
                     KFKKLDDSIHKEHYDRMHQKLTKTRNMLRKW"
     gene            1996..>3213
                     /locus_tag="PLD76_04690"
     CDS             1996..>3213
                     /locus_tag="PLD76_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOU27332.1"
                     /translation="MSFFPPLSVADAIDKIDYNQYLLPAIQRDFVWSSSKIEMLFDSL
                     MQGYPIGSMLLWRVNGKNTENQRFYGILKKYRERYLIRSEEISTKNIPSFEVVLDGQQ
                     RLTALYLGLKGSYAYKGYNLAWKDTEYSIPTRHLYLNLKYKKNRRNDLNDSDDDRMYE
                     FTFLKPEEALNSDDNNKWFKVGDILGIKGTAQLNSCFKKNDIIDEEAQEILSRLHDIV
                     HLEKLIHYYLEESSDFTRALNIFIRINSGGISLSYSDLVMSTTIMGWMRLDAKKEING
                     LVDELVKEYGFPVNKDFILRTYLVLYSDDIKFRVQNFSIKNAKEFETYWEEIKLAIKE
                     VFQLVSDFGYAPSTLTSMNALLPIVYYLYKTDRVKGFCQKVGYKEDRALIKRWLHVVL
                     LHQIFGGQAEGVLK"
BASE COUNT          974 a          568 c          592 g         1079 t
ORIGIN      
        1 taccgaaggt ttagttcaac agtggtagct gttgcacaac tcccattttt agaaatacta
       61 tttccaaaac ataaaatttt gacctcgttt aatcactttt aagcggggtt tgttttttag
      121 ttaacagtca aaaatgctaa aatttagaga gcttacaatg gagttattga tgttttgaaa
      181 gactgattta caaaagcaaa gttcttccct tgttttagag atagtacttg ggctaaaata
      241 ctcggttttt tggtgataaa gcgtaagtat tttttcaagt tgtaggttaa acttgccatt
      301 aatacgtgtt tgtttgcgtt tttgattcct cgtgtattaa cgcgcttcat attcgtaaag
      361 ttgaccaacg ttccaatgac gggttctacc gttttgctgc gtacttttac cattttctta
      421 gcatatttct cgttttggtg agtttttggt gcatacggtc gtaatgttct ttgtggatgc
      481 tgtcgtctag ctttttaaac ttggtgcttt tgccacagca ttgttctctt agtggacagt
      541 ttttgcaatc tgtttcgcta cttcggtacg ttcgtttggt gtagccttta ctatccattt
      601 tttcgccttt gaaaagcagt ttagcttgct taccttcggg ttttgtgcat tgataatagt
      661 tttcttcttt gtggtaagta aaaccttctc gctctgattt gtattgtccg aagttgggga
      721 tgtaggcgtt gatgtttttt tcgtgcaaat acgccaaagc ttcgccactg ctgtaaccac
      781 catcggctaa aagttcttcg agttctattc cgttttcgtt taggtttttt tcggtaagtt
      841 ctacaatttg ttctaaacac tgactgtcgc gtttgtccgc aaaatcagaa cacgctcctg
      901 taatcacatg gtgtgcatca tctactgcga tttgtccaaa ataatttaat tgtctcgctt
      961 tgcctggttt tacgcttact ctggcatcgc tatctgtggg agaataatgg gtgtgattag
     1021 acaagtattt gggacggatg agattgccgt tttcatctat ctgattgctg ttgctatttg
     1081 ctggcattcc tttatacgct tctgctttcc aatcatggtg gcgttcaacg aatttcttgc
     1141 gggtggaagt cactttgtat tcgctgtttt cttcaaattc gttcacaaaa gcaccagcat
     1201 cttccaaaac ttccttctct accaaactat ccatactggc gttggctttg atgaaaacgc
     1261 tgtccaccgc ttggcgtttc cctcttacca ttcctttaga aacgcacatt ttcaagactt
     1321 ctttaaacaa gtttaaaaag acttcttcgc catataaacc gcgagttcgg ctaatggtac
     1381 tgtgccaagg aagctgctca tgtacatcat aacccaaaaa taatcgtatc gacaagctat
     1441 cactacaaaa agctatcaac tgtctatctg aattgatatt gtttaaatat cccaccaata
     1501 aaatcttgaa aacaaacgag aagaaagcat agctttctac gaagattgcg aacgaagttc
     1561 actggatcta tactctcttg tccttcttta ccataatact tttgtgtagc tttataaatg
     1621 aaatgtaaat cgagttccgt tagcaatttt ctgtaaaaat tatcctctgg aactaggtct
     1681 aataaattga ccgaaacaaa taattgtggc gtaaaattct tctttccttg catatttaaa
     1741 tttacgaaat atccaatttt tatacaagtt ttttgtatat ttgagttgtg caacaagcac
     1801 aagggttttg caatagtggg gcgaaactgc aaagttcaac ggcagttcct cggttcaact
     1861 tttgtgcaaa attgaagatt tgtgcttcga ttgccccacc atcgcaaagc cccgctccgt
     1921 tatgcctcat tgtataactg acaccgaaac agaaaccgag aagaaattaa atttataaac
     1981 taattaataa acaatatgtc atttttcccg ccattatcag ttgctgacgc aatcgacaaa
     2041 atagattaca atcaatatct acttcctgct attcaaagag attttgtttg gtcaagtagt
     2101 aaaattgaaa tgctatttga ctcactaatg caaggctatc caattggttc aatgctactt
     2161 tggcgtgtta atggaaagaa tactgaaaat caaagatttt acggaattct taaaaagtat
     2221 cgggaacgct acttgataag gtcagaagaa atatcaacta aaaatattcc aagctttgaa
     2281 gttgttttgg acggacaaca aaggctaaca gctttatatt taggactcaa agggtcatat
     2341 gcttataaag gttataactt ggcttggaaa gatacggaat attctatccc cacaagacac
     2401 ctatatctaa atctaaaata caaaaagaat aggcgtaatg atttaaatga cagtgatgac
     2461 gacagaatgt acgagttcac ttttctaaag ccagaagaag cacttaattc tgatgataat
     2521 aataaatggt ttaaggtagg tgacatttta gggattaagg gaacagcaca attaaattct
     2581 tgcttcaaaa aaaacgatat tattgacgaa gaagcacaag aaatattgag cagactacac
     2641 gatatagtgc atttagaaaa actaattcat tattacttag aagaaagttc tgacttcaca
     2701 agagcattaa acattttcat cagaataaat tcaggcggta tttcacttag ctattccgac
     2761 ttggtaatgt ctaccacaat tatgggatgg atgcgacttg atgccaaaaa agaaataaat
     2821 gggttagtag atgaattagt caaagagtac ggattccctg taaacaaaga ctttatatta
     2881 cgtacatatc tcgttctata cagtgatgat attaaattta gagttcagaa tttcagtata
     2941 aaaaacgcta aagaatttga aacatattgg gaagaaatta aacttgcaat taaggaagtt
     3001 tttcagcttg taagcgattt tggttatgct cccagtactt tgacctcaat gaatgcatta
     3061 cttccaattg tttactacct gtataagacg gacagagtta aaggattttg tcaaaaagta
     3121 ggatataagg aagacagagc attaataaaa aggtggttac acgttgttct tttacatcaa
     3181 atttttggtg gacaagctga aggagtttta aag
//
LOCUS       DAOSMY010000175         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_30350_length_3186_cov_3.541680, whole genome shotgun sequence.
ACCESSION   DAOSMY010000175 DAOSMY010000000
VERSION     DAOSMY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30350_length_3186_cov_3.541680"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLD76_04695"
     CDS             complement(<1..680)
                     /locus_tag="PLD76_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOU27333.1"
                     /translation="MSPTTKNWIIATRPWSFPASAMPALFAMAYVFYMQDSIESIHWG
                     YGVAALLGAVLFQASGNLIGDYFDYMYKVDRKESLGSSRMLVDGVFTPKTIFWFGIIV
                     LCMGILLGLFLWSQTGNDLLWIGIVGALGTFFYYKLKYMALGDVLIFVIYGPLIALGT
                     AFVMTNQLYWEVVVLSIPIAFLVVNILHANNTRDMRDDKLANIKTQALLLGEKGSRIQ
                     YTFLAIGA"
     gene            complement(683..2584)
                     /gene="mutA"
                     /locus_tag="PLD76_04700"
     CDS             complement(683..2584)
                     /gene="mutA"
                     /locus_tag="PLD76_04700"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HOU27334.1"
                     /translation="MAEKKLNLLAEFPPVTTEKWMEKITADLKGADFSKKLVWKTNEG
                     FDVMPFYRAEDIETLKNTEAIPGKFPFVRGTKANNDWYVRQDIVVENVDEANKKALDV
                     LNKGITSLGFKISYQDLSSSYIQSLLKGIAADCVELNFSICTDHAAELAGILVAYFQS
                     KGYDLVKLHGSINFDAMNAMLLRGKEYDKASIVSMAKAIIDAIAPLPFYRVVGVNATT
                     LSNAGAYIAQELGYALAWGNQYLSLLVEAGVDTSLAAKKIKFNFGVGGNYFMEIAKFR
                     AAKLLWALIVDAYQPICRRPNCDNTVNGICKCAARIRIHAQTSEFNKTVYDANVNMLR
                     TQTEAMSASLGGVDSLTVLPYDSVYKKSDDFSERIARNQQLLLKEESNFDKVADPASG
                     SYYIETLTNEIAQQAWKLFLEVEENGGFYAAIVAGNVQKAIKKTASTRLKNVSSRREV
                     LLGTNQFPNFNEQAITKVQKDNNASSCCTHNTSIEQLCAVRAADEFESLRFATEKATK
                     RPKAFMLTIGNLAMRLARAQFSCNFFACAGYDVVDNLGFKTIEEGIAAAKKANADIVV
                     LCSSDDEYAELAPQAFDLAKGNAILVIAGAPACTDELKAYGIEHFINVKSNVLDTLRT
                     FNGLLTINQ"
     gene            2711..>3186
                     /locus_tag="PLD76_04705"
     CDS             2711..>3186
                     /locus_tag="PLD76_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HOU27335.1"
                     /translation="MTELNKEEEKFIVSLKRKFNKAIYQYGLISDGDVILVGLSGGKD
                     SLALVELLGERKKIFKPSFSVVAAHITLDNIPYTSDLAYLKAFCEQYDVPFVHRNSSF
                     DASTDKRKSPCFLCSWTRRKQLFDIAKELGCNKIALGHHQDDILETLLMNMSFQGA"
BASE COUNT         1039 a          640 c          506 g         1001 t
ORIGIN      
        1 tacgctccaa tagctaaaaa ggtatattga atgcgcgaac ctttttcgcc aagcaacaag
       61 gcttgtgttt tgatatttgc caatttatca tctctcatat cacgtgtgtt gtttgcatgt
      121 aaaatattca caaccaaaaa agcaatcgga atacttaaca ctacaacctc ccaatataat
      181 tgattcgtca ttacaaaggc tgttcctaaa gcaatgagcg gtccgtaaat aacaaaaatc
      241 aagacatcgc ccaaagccat atactttagt ttgtaataaa aaaaggtacc caaagcacca
      301 acgataccaa tccatagcaa atcgttgcct gtttgcgacc ataaaaacaa accgagcaaa
      361 atacccatac ataatacgat tattccaaac cagaatattg ttttgggcgt aaatacacca
      421 tctaccaaca tacggctcga accgagcgat tcctttctat ctactttata catgtaatcg
      481 aaataatcgc caataagatt tccgcttgct tgaaacaaca cagcacccaa taaagcagct
      541 actccatacc cccaatggat ggactctatg ctatcttgca tgtaaaaaac atacgccata
      601 gcaaacaaag cgggcattgc cgatgccgga aacgaccatg ggcgtgtagc tattatccaa
      661 ttttttgtag ttggactcat ctctattgat tgatagttaa taaaccgtta aacgtacgta
      721 aagtatccaa cacattggat ttaacattga taaaatgctc aataccatag gcttttaact
      781 catcagtaca agctggagct cctgctatca ctaaaatagc gtttcctttt gctaaatcaa
      841 aagcttgagg ggctaattca gcatattcat catcacttga gcataatacc actatatcag
      901 cattggcttt tttagccgct gctatacctt cttcgatggt tttaaatcct aaattatcta
      961 ctacatcata ccctgcacaa gcaaagaaat tgcacgaaaa ctgagccctt gccaaacgca
     1021 tagccaagtt accaatggtt agcataaatg cttttggacg cttagtcgct ttttcggtag
     1081 caaaacgtaa ggattcaaat tcatcagctg cacgtactgc acataactgt tcgatagatg
     1141 tattatgtgt gcaacaagat gatgcattat tatctttttg aactttagta attgcttgct
     1201 cgttgaaatt aggaaattga tttgttccaa gtaacacttc acggcgagat gaaacatttt
     1261 ttaaacgcgt acttgcagtt tttttaattg ctttttgtac attacctgct acaatagctg
     1321 cataaaaacc accattttct tctacttcta aaaataattt ccaagcttgt tgagctattt
     1381 cattggttaa tgtttcgata tagtacgaac cagaagctgg gtcagccacc ttatcaaaat
     1441 ttgattcttc tttcaataat aattgttggt tacgagcaat acgctccgaa aaatcatctg
     1501 attttttgta cactgaatcg taaggcaata cggttagtga atctacccca cccaacgaag
     1561 cactcatagc ttccgtttgt gtgcgaagca tatttacatt ggcatcatat actgttttgt
     1621 taaattctga tgtttgtgca tgaatgcgta ttctggcagc acatttgcaa ataccattta
     1681 ctgtattatc acaattagga cgacgacaaa ttggttggta tgcatctaca atcaatgccc
     1741 acaacaattt ggcagcacgg aatttggcaa tttccataaa ataattgcca cctacaccaa
     1801 aattaaattt aattttttta gcagccaacg atgtatctac acctgcctct accaataatg
     1861 aaagatattg atttccccaa gccaatgcat atcctaactc ttgagctata taagctccag
     1921 cattgcttaa ggttgtagca ttaacaccca caacacggta aaaaggaaga ggagcaattg
     1981 catcaataat tgcttttgcc atcgatacga tacttgcttt atcatattct ttaccacgca
     2041 ataacatagc attcatagca tcaaagttga tggagccatg caatttaact aagtcatatc
     2101 cttttgactg aaaataagca actaatatcc cagccaattc agcagcatga tcggtgcaaa
     2161 tactaaaatt taactctaca caatcagcag caataccttt taaaagtgat tggatatagc
     2221 tacttgacaa atcttgatag ctaattttaa aaccaagcga agtaattccc ttgttaagaa
     2281 catccaatgc ttttttgttc gcctcatcca cattttctac tacaatatcc tgacgaacat
     2341 accaatcatt gttagccttt gtacccctta caaacggaaa ctttcctgga atagcctctg
     2401 tgtttttcaa agtctcaata tcctctgcac gataaaacgg cattacatca aaaccctcat
     2461 tggttttcca aactaatttt ttactaaaat cagcaccttt caagtcagcc gtaattttct
     2521 ccatccactt ttcggttgtt accggtggaa attcagccaa taaattcaat tttttttctg
     2581 ccataataat ataataattg tataattttc taaaactcag caaaattagt tgttttaatt
     2641 aatataatga atttttaggc ttgtaaattt taataaaata agtacttttg ttgtgaaaat
     2701 agcattcaaa atgaccgaat taaacaaaga agaagaaaaa tttatcgttt cgttaaaacg
     2761 gaaattcaat aaagctatct atcagtacgg tttaatttca gatggagatg ttattttggt
     2821 ggggctttcg ggagggaaag attcattggc attggtagaa ttattaggtg agcgaaaaaa
     2881 aatatttaag ccttctttta gtgttgttgc tgcacatatt acgttggata atatacctta
     2941 tacatcagat ttggcttatt taaaagcgtt ttgcgaacaa tatgatgtac cttttgttca
     3001 tcgcaatagt agttttgatg ccagtactga taagcgtaaa tcaccttgct ttttgtgttc
     3061 gtggacacgc agaaaacagc tttttgatat tgccaaagag ttgggttgca acaaaattgc
     3121 attggggcat caccaagacg atatattgga aacattgttg atgaatatga gttttcaagg
     3181 agcatt
//
LOCUS       DAOSMY010000176         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_30722_length_3158_cov_4.282307, whole genome shotgun sequence.
ACCESSION   DAOSMY010000176 DAOSMY010000000
VERSION     DAOSMY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30722_length_3158_cov_4.282307"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2802
                     /gene="ppdK"
                     /locus_tag="PLD76_04710"
     CDS             82..2802
                     /gene="ppdK"
                     /locus_tag="PLD76_04710"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOU27336.1"
                     /translation="MDKKRVYFFGNKTAEGNGSMKELLGGKGANLADMNLIGVPVPPG
                     MTITTEVCNEYFLIGQEEVIKTIKPEVEKAMKQIEDAVAGPKFGDNKNPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEAVETIAQKSGNPRFAWDSYRRFVQMYGDVVLGMKPESKQ
                     DEDPFEVIIEKMKHEKKVELDTQLTTEDLKELVAKFKKAVKDVTGKDFPVSPWEQLWG
                     AVMAVFNSWMNERAILYRKMNKIPAEWGTAVNVQAMVFGNMGNNSATGVAFTRDASTG
                     EDIFNGEYLINAQGEDVVAGIRTPQEITIEGSRRWAKMQGISEADRLAKYPSLEETMP
                     SAYADLNAIQQKLEDHYTDMQDIEFTIQDGKLWMLQTRNAKRTGAAMVRVAMEMLKQG
                     IIDEKTALLRCEPEKLDELLHPVFDTKALAQAKALSQGLPASPGAATGQVVFFAEDAE
                     AWAAEGKKTILCRVETSPEDLKGMYAAEGILTARGGMTSHAAVVARGMGKCCISAANG
                     LVIDYKTRTMTVGGATIKEGEWVSLNGTTGILYAGKVETKAAELSGDFAELMKIADKY
                     ARLQVRTNADTPHDAQVARNFGAQGIGLTRTEHMFFDGDKIKAMREMILAQDEAGRRK
                     ALVKLLPLQREDFEGIFEAMNGLPVTVRLLDPPLHEFVPHDPKGQLEMAHEMGLNVDE
                     IAARVHALSESNPMLGHRGCRLGNTYPEITETQTRAIIEAALNLKKKGIVAIPEIMVP
                     LTGILHEFTAQKTIIDATAKQVFEERGDSIEYKVGTMIEIPRAALTAQRIAEQAEFFS
                     FGTNDLTQMTFGYSRDDVAKFLPIYLEKGILKNDPFQVLDQKGVGQLITMATVNGRAV
                     RPNLKVGICGEHGGEPSSVEFCHRTGLNYVSCSPFRVPIARLAAAQAAIR"
BASE COUNT         1031 a          542 c          670 g          915 t
ORIGIN      
        1 aatcagaaaa attttatctt tgcactctat ttgataaaat atacgtgttt attcaaaata
       61 attttttaat ttattaattt tatggacaag aaaagagttt atttttttgg aaacaaaacc
      121 gctgaaggta acggctctat gaaagagtta cttggtggaa aaggggctaa cttggcagat
      181 atgaacctga ttggtgtgcc tgttcctccg ggaatgacaa ttacaacaga ggtgtgtaat
      241 gagtattttt taatcggtca agaagaggtt attaaaacca ttaagccaga agttgaaaaa
      301 gcaatgaaac aaattgaaga tgctgttgct ggtccaaaat ttggcgataa caaaaatcct
      361 ttattggtat ctgtacgttc tggagctaga gcttctatgc caggtatgat ggatactatt
      421 ttgaacttag ggttaaatga cgaagctgtt gaaactatcg ctcaaaaaag tggtaatcca
      481 cgtttcgcat gggattctta ccgtcgtttt gtacaaatgt atggcgacgt ggtactaggc
      541 atgaaaccag aatcaaaaca agatgaagac ccatttgaag taatcatcga aaagatgaaa
      601 catgaaaaaa aggtagaatt agatactcaa ttaacaaccg aagatttaaa agaattggtt
      661 gctaaattca aaaaagctgt aaaagacgtt acaggtaaag attttccagt ttctccatgg
      721 gaacaacttt ggggtgctgt tatggcagta ttcaatagct ggatgaacga aagagctatt
      781 ctgtatcgca aaatgaataa aattcctgct gaatggggaa cagctgttaa cgtacaagca
      841 atggtatttg gtaacatggg taacaattct gctacaggtg tggcatttac tcgtgatgct
      901 agtacaggtg aagatatttt caatggcgaa tatttaatta atgctcaagg tgaagacgtg
      961 gtagcgggta ttcgtacacc acaagaaatt accatcgaag gttctcgtcg ttgggctaaa
     1021 atgcaaggca tcagtgaagc tgatcgtcta gctaaatacc cttcattaga agaaacaatg
     1081 ccttcagctt atgctgactt aaacgctatt caacaaaaat tagaagacca ctatacagat
     1141 atgcaagata tcgaatttac tatccaagat ggtaaattgt ggatgcttca aacacgtaac
     1201 gctaaacgta ctggtgctgc tatggtaaga gtagctatgg aaatgcttaa acaaggcatt
     1261 atcgacgaaa aaacagcatt attgcgttgc gaaccagaaa aattagacga acttttacat
     1321 ccagtatttg atacaaaagc attagcacaa gcaaaagcct tatctcaagg tttacctgct
     1381 tctccaggtg ctgctactgg tcaagtagta ttctttgcag aagatgcaga agcatgggct
     1441 gctgaaggta aaaaaactat cctttgccgt gtagaaacat ctccagaaga ccttaaaggt
     1501 atgtatgctg ctgaaggtat tcttactgct cgtggtggta tgacatcgca cgctgctgta
     1561 gtagctcgtg gtatgggtaa atgctgtatc tctgctgcta atggtttggt tattgattac
     1621 aaaactcgta caatgacagt aggtggagct accattaaag aaggtgagtg ggtatcatta
     1681 aatggtacta ctggtattct ttatgctggt aaagtggaaa ccaaagcggc tgaattaagt
     1741 ggtgactttg ctgaattaat gaaaatagct gacaaatatg cacgtttaca agtacgtacg
     1801 aatgctgata cgcctcacga tgctcaagta gctcgtaact tcggtgctca aggtattggt
     1861 cttactcgta ctgaacacat gttcttcgat ggtgataaaa ttaaagcaat gcgtgaaatg
     1921 attcttgctc aagatgaagc aggtcgccgt aaagctttgg taaaattatt accattacaa
     1981 agagaagact ttgaaggtat cttcgaagca atgaacggac ttccagtaac ggttcgttta
     2041 cttgacccac ctttgcatga gtttgttcct cacgatccaa aaggtcaatt ggaaatggct
     2101 cacgaaatgg gcttaaatgt tgacgaaatt gctgctcgtg tacatgctct ttctgaatca
     2161 aacccaatgt taggtcaccg tggttgtcgt ttaggtaaca catacccaga aattactgaa
     2221 acacaaactc gtgctatcat cgaggctgct cttaacttga aaaagaaagg cattgtagct
     2281 attccagaaa tcatggtacc tcttacaggt atcttacacg agtttactgc tcaaaaaact
     2341 attatcgacg ctactgctaa acaagtattt gaagaaagag gcgattctat tgaatataaa
     2401 gtaggtacaa tgattgaaat tccacgtgca gcgttaacag ctcaacgtat tgctgaacaa
     2461 gcagaattct tctcatttgg tactaacgac ttaacacaaa tgacatttgg ctatagccgt
     2521 gatgacgttg ctaaattctt accaatttat ttggaaaaag gaattttgaa aaacgaccca
     2581 ttccaagtgc ttgaccaaaa aggtgtagga caattaataa ctatggctac tgtaaacggt
     2641 agagctgttc gtccaaacct taaagtaggt atttgtggtg agcatggtgg tgagccatct
     2701 tcagtggaat tctgccacag aactggtttg aactacgtaa gttgttctcc attccgtgtg
     2761 ccaatcgcac gtttggctgc tgctcaagcg gctattcgct gactgacaga agacattgaa
     2821 acaatggaat aaataaggta gagagcttgc taatgtagca agctctctgt ttttttctac
     2881 tatgggtttt taaagagaaa ggtctctcgt ttatcgtatc gttttttaag acttaacttg
     2941 gcatgtttat aacaaataaa attcattgta caaagacgat actacaaaaa tgtttatctt
     3001 tgcaaaagaa ctacagggta aaatcttgcg gtaactatac agaatctttt ctgtaaaaac
     3061 aaaggaataa gtttaaatct gtattttgca gttgcagtga aattaacgat gaaccataca
     3121 aaactgtatc ggcacggatt acaaatccgc gccagcgt
//
LOCUS       DAOSMY010000177         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_30744_length_3157_cov_3.632495, whole genome shotgun sequence.
ACCESSION   DAOSMY010000177 DAOSMY010000000
VERSION     DAOSMY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30744_length_3157_cov_3.632495"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..899)
                     /locus_tag="PLD76_04715"
     CDS             complement(186..899)
                     /locus_tag="PLD76_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOU27337.1"
                     /translation="MEFLQTIVSQSEIPILTAFVLGLMTAISPCPLATNITAIGFISK
                     DMQQKRKVFINGLVYTLGRAVSYTVLGVMLIVLFQQGVSLFKVQRFVSLYGMYLLIPF
                     LLVMGVFMLDFIKISLPGFSRMTLKMEEKASSGSAWSSFLMGIVFALAFCPYSGVLFF
                     GALIPLSVSVSAGYALPFVFAIATGLPVVIFAWIFAFSISSVSLFYNKIKVFEKWFRR
                     VVGVLFIVVGLYFAAILFF"
     gene            complement(899..1294)
                     /locus_tag="PLD76_04720"
     CDS             complement(899..1294)
                     /locus_tag="PLD76_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27338.1"
                     /translation="MKKTLILAVLALMSVTVVVAQKAEVLYFKADMPCCKARACNNLE
                     ADIKAVVEKNYPKGDVVFKQVRIADEANKALVEKHQAKAQTIVIVGKKKKKKQQAIDV
                     SEVVRGCVRSGDKTQLETKLVEAINKAIK"
     gene            complement(1299..1712)
                     /locus_tag="PLD76_04725"
     CDS             complement(1299..1712)
                     /locus_tag="PLD76_04725"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOU27339.1"
                     /translation="MKQKISILFVVFLLFVSCAKTASTEQVAQENDVAKVTVYYFHGK
                     QRCPTCVSIQQVAQSAVDTFFANNEDVRFVEVDFSVKKNDALAEKYEVAFSSLIIATA
                     DAYSDITDQAFALATSNPEQLKTIINQEVTKYLNQ"
     gene            complement(1725..1961)
                     /locus_tag="PLD76_04730"
     CDS             complement(1725..1961)
                     /locus_tag="PLD76_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU27340.1"
                     /translation="MEIKVLGPGCPKCKSLEKLVQETVQEMQLDATITKVEDIMEIMQ
                     YNIIATPALVINGKVVLKGVLPSKVQLQTILSTN"
     gene            complement(1977..2288)
                     /locus_tag="PLD76_04735"
     CDS             complement(1977..2288)
                     /locus_tag="PLD76_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOU27341.1"
                     /translation="MAKKNFTEEQEKLARYNKALGHPVRVFIMEYLVKNASKCCYSGD
                     MIENLPIARSTLSEHLKELKKAGFIQGEINPPYIKYCINKENWEEAKQMVMDFYSINN
                     D"
     gene            complement(2408..>3157)
                     /locus_tag="PLD76_04740"
     CDS             complement(2408..>3157)
                     /locus_tag="PLD76_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOU27342.1"
                     /translation="PVVISLLNALTGIAATMAGFIYQNQAMILGGIFVGAAGMILTLL
                     MCKAMNRSLIKVIIGNFGGGGVGVDKEVGEMKEISASDASILLNYSQKVVIIPGYGLA
                     VAQAQHIVHELDQLLESKGVDVRYAIHPVAGRMPGHMNVLLAEADVPYDKLVEMDAIN
                     PDMPNVDVAVVIGANDVVNPAASDDPSSPIFGMPIIGAHLAKHVIVLKRGAGKGYAGI
                     ENHLFYNEKTRMYYGNAKDSLQKLVAEIKSM"
BASE COUNT         1002 a          648 c          520 g          987 t
ORIGIN      
        1 gtaccatcgg gataaatcga gcgtagcatc aatagccgta gcaaaaacaa cactttcgag
       61 aggtaaaaaa taaataccta aaaatactac gacaatggca gctagtattt tcaattccgt
      121 tttgagttcc atagttttat aattatttcg ttaaaacacg aaatgaatag gtaaaaaatt
      181 ttattttaga aaaataaaat agcagcaaaa taaagtccaa ccacgataaa aagtacacca
      241 actacacgtc taaaccattt ttcgaatact tttattttgt tgtaaaacaa gcttacgctg
      301 gatatactaa aagcaaatat ccaggcaaaa ataaccacag gcaaaccagt agctatagca
      361 aacacaaaag gcaaggcata ccctgctgat actgaaacag acaacggaat aagtgcccca
      421 aaaaataaca caccactata cggacaaaaa gccagagcaa atacaattcc catcaaaaag
      481 gaactccatg cagaacctga cgatgctttt tcttccattt ttaatgtcat tcgactaaat
      541 cccggtagcg atattttaat aaaatcgagc ataaaaacac ccattaccaa tagaaaagga
      601 ataagtaaat acatgccata caaactaaca aaacgctgaa ctttaaaaag gcttacaccc
      661 tgctgaaaca acacaatcag cataacaccc aatacagtat aagacacagc cctacccaat
      721 gtatatacaa gtccattgat aaataccttg cgtttttgtt gcatgtcttt gcttataaac
      781 ccgatagccg tgatattagt agccaaaggg caagggctaa tggcagtcat caaccctagc
      841 acaaaggctg ttaaaatcgg aatttcagat tgtgaaacaa tggtttgtaa aaactccact
      901 acttaatggc tttattgatt gcttctacta atttggtttc gagttgagtt ttatcaccac
      961 tgcgtacgca accacgcact acttctgata cgtcaattgc ttgttgtttt ttctttttct
     1021 ttttgcctac aataacgatg gtttgagctt tggcttggtg tttttctacc aaggctttgt
     1081 ttgcctcatc agcaatacga acttgcttaa atactacatc tcctttggga tagttttttt
     1141 ctaccacagc ttttatgtcg gcttctaagt tattacaagc acgagctttg caacaaggca
     1201 tatcggcttt aaaataaaga acttcggctt tttgagctac aactactgta actgacatca
     1261 gtgccaatac ggctaaaata agtgtttttt tcatactttt attgatttaa atacttggta
     1321 acttcttggt ttattattgt ttttaattgt tctggattac tagtggcgag agcaaaagct
     1381 tggtcggtaa tatcgctgta agcatctgct gtagcaatga ttaacgagct aaatgctact
     1441 tcgtattttt cggctaaagc atcatttttt ttgacagaga aatctacttc tacaaaacgt
     1501 acatcttcgt tgttggcaaa aaacgtatca acagccgatt gggctacttg ctgaatggaa
     1561 acgcaagtag ggcaacgttg cttaccatga aaataatata cagttacttt ggctacatcg
     1621 ttttcttggg ctacttgctc tgtagatgcg gttttggcgc acgaaacaaa caagagaaaa
     1681 acaacaaata agatactgat tttctgtttc atagctaggt gcgtttagtt ggttgacaaa
     1741 attgtttgta attgcacttt cgagggcaat actcctttca gcacgacttt accgttaatc
     1801 actaaagcag gagtggcaat aatattgtat tgcataattt ccataatatc ttctaccttg
     1861 gtaatggtag catcaagttg catttcttgc actgtttctt gcactaactt ttctaaactt
     1921 ttacatttcg gacaccctgg tccaagtact ttaatttcca tactcattat cttaaattag
     1981 tcgttattga ttgaataaaa atccatcacc atctgtttgg cttcttccca gttttctttg
     2041 ttaatgcaat acttaatata cggcgggttg atttcccctt gtataaaacc tgcttttttc
     2101 aattctttaa ggtgttcgga caaggtagag cgtgcaatgg gtaaattctc tatcatatcg
     2161 ccactatagc aacattttga ggcattctta actaagtact ccataataaa tacacgtaca
     2221 ggatgaccta atgccttgtt gtaacgagct aatttttctt gttcttctgt aaagtttttc
     2281 tttgccataa ttcgtatttc gtaaaaagac gaaacaaaga taaataagtt tacggtacaa
     2341 ggcataaaaa aactgctaaa ttaattttat ttagcagttt taacgtttga atagggaaga
     2401 taaacgatta catactctta atttcagcaa ccagtttttg caacgaatct ttggcattgc
     2461 cataatacat acgtgttttt tcattgtaaa acaagtggtt ttctattcct gcgtaccctt
     2521 tacctgcacc tcttttaagc acaattacat gtttagctaa atgtgctcca ataatgggca
     2581 ttccaaatat gggcgaagag gggtcgtcgc tagctgcagg gtttaccaca tcgttggctc
     2641 caattactac cgccacatct acattgggca tatctggatt aatagcatcc atctccacca
     2701 atttgtcgta tggcacatca gcctctgcca ataacacatt catgtgccct ggcatacgac
     2761 cagctacagg gtgaatggca taacgtacat caactccttt ggattcgagc aattggtcaa
     2821 gttcatgcac aatatgttgt gcttgtgcca ccgccaatcc ataacctgga attattacta
     2881 ctttttgtga gtaatttagc aaaatagatg catctgaagc agatatttct ttcatctcgc
     2941 ctacttcttt atctacgccg acaccgccac caccaaagtt gccaataatt actttaatca
     3001 aggacctatt cattgcctta cacatcaata aggtaagaat cataccagct gcacccacaa
     3061 aaataccccc taatatcata gcttgatttt ggtaaataaa gcccgccatg gtagcagcta
     3121 tccctgttaa agcgtttaat aatgatatta ctacagg
//
LOCUS       DAOSMY010000178         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_30844_length_3148_cov_3.347882, whole genome shotgun sequence.
ACCESSION   DAOSMY010000178 DAOSMY010000000
VERSION     DAOSMY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30844_length_3148_cov_3.347882"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLD76_04745"
     CDS             <1..465
                     /locus_tag="PLD76_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOU27343.1"
                     /translation="PLSTLQIVLAKYFAGVVLVVLSLLPTLVYVYSVYQLGLPQGNID
                     MGGFLGSFIGLIFLGAGYTAIGLWASSVSDNQIVSFLVAALLSFLLYYGFELLSLFFT
                     SAYVQDMVSQMGMSYHYDSMSRGVIDSRDVLYFVTISVVFLFATTWFVTKRK"
     gene            529..2253
                     /gene="gldG"
                     /locus_tag="PLD76_04750"
     CDS             529..2253
                     /gene="gldG"
                     /locus_tag="PLD76_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOU27344.1"
                     /translation="MKRPILITLVVLVLLHVVSAFYFFRIDLTAEQRYTLSPNTKKLL
                     RTLDNTMDVTIYLSGDLNMGFLRLQKATTEMLQEFSRYSDQPIAIQMVNPSKASNEKA
                     RFLQYNALEQQGMVPTIVNEKDAEGKFIQKVVFPWAEMVYQGDTVRVNLLKNIPGKIG
                     DENLHISIESLEYELTDAIRRLANKHTAERVAFIEGHGELSEMQVFDITAALAQYYHV
                     DRGQIGADVRILDEYKVVIVAGATERYTESEKFILDQYIMNGGRVLWLLDGVRTSQEA
                     LTTSATTIGIVNDVNLDDQLFTYGIRIAPILLADKQCAQVRVNVATMGQTPQFELAPW
                     YYFPLLQTSPYHVITKNLSPVRAEFASAVQFVGENNAVTKELLLASSTATGIQTAPME
                     LSLSMINNPPSDEFFQYSFVPVAAIMSGSFESVFTNRMVPQDLIVPQVYKIRTKSNKT
                     KMVVVADADIIRNEVQVSANDTVALPLGYDRHVNKQYGNRDFILNTVNYLADDEGWMN
                     LRAREFTLRLLNKQAIESQRVFWQIINVVLPLVLLIVCILLYQRSRKMRYGNTRKSSS
                     IKAFFTKK"
     gene            2328..>3148
                     /gene="dnaN"
                     /locus_tag="PLD76_04755"
     CDS             2328..>3148
                     /gene="dnaN"
                     /locus_tag="PLD76_04755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU27345.1"
                     /translation="MKFAVSSSHLYERLSSVSRVINTKNTLPILNYFLFSIQGKELSI
                     TASDMETTLITSMELEEVEGEGKVAILAKNLLDILREFADQPLTITIDTASFSVVITS
                     AQGKFSFIGQSGEEYPKMHELDADNTVSFVAPASVLLDGISSTVFATADDELRPVMNG
                     ICFDVTTEELTLVGTDAHKLARFKTTAIKGEKDAMFVLPKKPASLLKSILVKETAPVN
                     VSFDTKNSIFTLSNYKMVCRQIDARYPNYNAVIPKSGPTKIIVDRLALLSGVKRVS"
BASE COUNT          954 a          525 c          665 g         1004 t
ORIGIN      
        1 ccactcagta ctttgcagat tgtattagct aaatattttg caggcgtagt attggttgtg
       61 ttgtctttgc tacctacctt ggtatatgtg tatagtgtgt atcagttggg cttgccacaa
      121 ggcaatatcg atatgggtgg atttttgggt tcttttattg gacttatttt tttaggagct
      181 ggttatacgg ctattgggtt atgggcttcg tctgttagtg ataaccagat tgtatctttt
      241 cttgtggcag ctttgttgtc atttttattg tattatggtt ttgaattact atctctattt
      301 tttacttcgg catatgtgca agatatggta tcgcaaatgg gcatgagcta ccattacgat
      361 tcaatgagtc gtggtgtaat tgatagtaga gatgtgttgt attttgttac catcagtgtt
      421 gtttttctgt ttgctactac ttggtttgtg actaaaagaa aatagcaagg gagtgaaaaa
      481 ggaatgcaaa aaacgaaatt taccttagag aaagcataaa aaatgtatat gaaaagacct
      541 attctcataa cgcttgttgt attagtgttg ctacatgtag tatctgcatt ttattttttt
      601 cgtattgact taacagcaga gcaacgctat accttgtcgc caaatactaa aaaattacta
      661 cgtaccctag ataatacaat ggatgtaacc atttatttat caggcgattt gaatatgggt
      721 tttttgcgat tgcaaaaagc gactactgaa atgttgcaag agttttctcg ttattcagac
      781 caacccatag ccatacagat ggtgaatcca tccaaagcga gcaacgaaaa ggctcgtttt
      841 ttgcaataca atgcgttgga gcaacaaggt atggtgccta ctattgtaaa cgaaaaagat
      901 gctgagggga aatttataca aaaagtagtt tttccatggg cagaaatggt gtatcaagga
      961 gatactgtac gtgttaattt gcttaaaaac atacctggta aaatagggga tgagaatttg
     1021 catatttcga ttgaaagtct tgaatatgag ctgactgatg ctattcgtag gttggcaaat
     1081 aaacataccg ccgaacgtgt ggcgtttatc gagggacatg gcgaattgtc cgaaatgcag
     1141 gtgtttgaca tcacagccgc tttggcacag tattaccatg tagatagagg gcaaattggt
     1201 gctgatgtgc ggattttaga tgaatataag gtggtaattg tagctggagc aacagaacgt
     1261 tacaccgaat cagagaaatt tattctcgac caatatatta tgaatggcgg acgcgtactt
     1321 tggttgctcg atggtgtgcg aacatcgcaa gaagctctta ctacatccgc tacaacgata
     1381 gggatagtga atgatgtgaa tttggacgat cagcttttta cctatggtat ccgtattgct
     1441 cctattttac tagctgacaa acaatgtgct caagtgcgtg ttaatgttgc cactatgggg
     1501 caaacgcctc agtttgaact ggctccgtgg tattatttcc ccttgttaca aacatcccca
     1561 tatcatgtta ttaccaaaaa cctctcgcct gtgcgtgccg agtttgcttc tgccgtacaa
     1621 tttgtaggcg aaaataacgc agtaacaaaa gaattattgc tagcaagctc tactgctacg
     1681 ggtatccaaa cggctcccat ggagttaagc ttgtcgatga ttaataatcc gccctctgac
     1741 gagttttttc agtatagttt tgtgcctgta gcggctatta tgtcgggtag ttttgagtcg
     1801 gtatttacca accgcatggt gccccaagat ctcatagtgc cccaagtata taaaattaga
     1861 acaaagagca ataaaacaaa aatggtagta gtggctgatg ctgatattat tcgtaacgaa
     1921 gtgcaagtgt ctgctaacga tacagttgca ttgcctttag gctacgatag gcacgtaaat
     1981 aagcaatacg gaaatcgcga ctttatttta aataccgtga attatttagc tgacgacgaa
     2041 ggatggatga atttgcgtgc aagggaattt acattacgtt tgttaaacaa acaagccatt
     2101 gaatcacaac gtgttttttg gcagattatc aatgttgtgt tgccattagt tttgctcatt
     2161 gtttgtatct tgttgtatca aagaagtaga aaaatgcgtt atggaaatac gcgaaaatct
     2221 tcatcgataa aagctttttt tactaaaaaa taatgtatat ttgtcaactg attttagaca
     2281 atatagataa ataagtatcg ataaactaaa tttaaaacta catacaaatg aaatttgcag
     2341 tatcatctag ccacctttac gaaagactaa gttcagttag tcgcgttatt aacactaaaa
     2401 ataccttacc aattctaaat tattttttgt tttctataca aggcaaagag cttagtatca
     2461 ccgcttcgga tatggaaaca acccttatta catcaatgga gttagaagag gtagagggag
     2521 aagggaaagt agccattttg gcaaaaaact tactagatat tttacgcgaa tttgccgatc
     2581 aaccacttac tattaccatt gatacagcta gtttttcggt agtgattaca agtgcacaag
     2641 gtaaatttag ttttattgga caatcgggcg aagaatatcc taagatgcac gaactggatg
     2701 cagataatac cgtttctttt gttgctccag ctagcgtttt gttagatggt atatcgagta
     2761 cagtgtttgc tacggcagat gacgaattgc gtcctgtaat gaatggtatt tgttttgatg
     2821 taaccactga ggaacttacc ttggtaggta cggatgctca taagttggca cgttttaaaa
     2881 caactgccat caaaggcgaa aaagatgcta tgtttgtatt gcccaaaaaa ccagcctcat
     2941 tgctcaaaag tattttagta aaagaaacag caccagtaaa cgtatcgttt gatacaaaaa
     3001 attcaatttt taccttatca aattacaaaa tggtgtgtcg ccaaatagat gctcgttacc
     3061 cgaattacaa tgctgttata cccaaaagcg gacctaccaa gattattgtt gatagattgg
     3121 cgttgctttc tggtgtaaaa agagtatc
//
LOCUS       DAOSMY010000179         3132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31085_length_3132_cov_3.167696, whole genome shotgun sequence.
ACCESSION   DAOSMY010000179 DAOSMY010000000
VERSION     DAOSMY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31085_length_3132_cov_3.167696"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLD76_04760"
     CDS             <1..454
                     /locus_tag="PLD76_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU27346.1"
                     /translation="DDLYENATTLQQIVNAFYEQNCAMVIGSYSMTDFALQPIPPGLI
                     DHKEWTPENGRNNALRINGLGAPRAFYTPVLREIKVPNTSYGEDYALGLQISRNYQIG
                     RIYNSVYLCRRWNENSDAALDIEKMNAHNAYKDKLRSIEMMARIAMNK"
     gene            457..834
                     /gene="folK"
                     /locus_tag="PLD76_04765"
     CDS             457..834
                     /gene="folK"
                     /locus_tag="PLD76_04765"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU27347.1"
                     /translation="MNTHHYILLLGSNFLAHKHLSIAIELLQQEFGIYRTSHEMETKA
                     IDMPNDYPFVNKAVCIQTRLEPTELIQKIKEMEKQLGRSDEKTLQGIIPIDIDVILCD
                     KKVLHSDYETKNYVKKLVEEVSV"
     gene            880..1827
                     /locus_tag="PLD76_04770"
     CDS             880..1827
                     /locus_tag="PLD76_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU27348.1"
                     /translation="MKKHLLCLIALFYFFISTTNSQENTLPRVSSGTIIRHVDFTSNY
                     ISARNVDVWLPDNYSPNQRYKVLYMHDGQMLFDANTTWNKQEWGVDETITRLIANDSI
                     LSTIVVGIWNSGRTRHSDYFPQKPFERLPVSTQDSLILLGRRQNNVSLFAERVQSDNY
                     LRFIVEELKPFIDKTYATLTEPEHTFIAGSSMGGLISMYAVCEYPEVFGGAACLSTHW
                     IGSFQTEGNPIPAQFFAYLKENVPSPDTHKFYFDFGTETLDVHYEPYQQQVDAIMQAK
                     GYNSTNWITRKFEGKDHSENAWRERLHIPLTFLLPIQFN"
     gene            1851..2324
                     /locus_tag="PLD76_04775"
     CDS             1851..2324
                     /locus_tag="PLD76_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOU27349.1"
                     /translation="MKTENNSSCCTSSSEYSQQIGKLALRLLVGGFMLTHGLPKLQNF
                     EYLSTVFPDPLGVGSSLSLGMIVFAEVVCSILLMLGLFTRLSTIPLIIGMGVAAFFIH
                     ANDPFSGKEMALLYMGIYVIIALIGAGKYSADYLLRNQYAKICSSYCKPKAENTK"
     gene            2467..2904
                     /locus_tag="PLD76_04780"
     CDS             2467..2904
                     /locus_tag="PLD76_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU27350.1"
                     /translation="MSLVKFSNNYPTIFDRFFDNNFYDWTSENYSTTNTTVPSANIKE
                     SAEKFEIHIAAPSFAKDDFKIEVNNNVLTVCSEKKVENETKEGERFTRKEFSYQSFCR
                     SFTLPISADTEKISANYENGILAVDIPKKEEAKPKPSKIIEIK"
BASE COUNT         1094 a          563 c          541 g          934 t
ORIGIN      
        1 cgatgactta tacgaaaacg caacaacact acaacagata gtaaacgcat tttacgaaca
       61 aaattgtgcc atggtaattg gctcgtatag catgacggat tttgcccttc aacccattcc
      121 tccaggacta attgaccaca aagaatggac acccgagaat ggaagaaaca atgcacttcg
      181 tatcaatgga ttaggagctc cacgagcgtt ttatactcct gtattaagag aaatcaaagt
      241 gccaaatacc agctatggag aagattatgc tttgggactt cagattagta gaaactacca
      301 aatcggacgc atttataatt cagtatattt gtgtcgtagg tggaacgaga attctgatgc
      361 agctttagat atagaaaaaa tgaacgctca taatgcatac aaagataaat tacgtagcat
      421 agaaatgatg gcacgtatcg ccatgaataa atagaaatga acactcacca ttacatctta
      481 ttgcttggca gcaatttttt ggcacacaaa catctgtcaa tagccattga attgctgcaa
      541 caagaatttg gtatttatcg cacctcgcac gaaatggaaa cgaaagccat tgatatgcca
      601 aacgactatc cctttgtaaa taaggctgtt tgcatacaaa ctaggcttga gccaacagag
      661 ctaatacaga aaataaaaga aatggaaaag caacttggtc gttcggatga aaaaaccttg
      721 caaggcatca ttcccattga tattgatgtg attctctgtg ataaaaaagt gttacattcg
      781 gattatgaaa caaaaaatta tgttaagaaa ttggtagaag aagtgagcgt ttgagaaaat
      841 tgtacgtata tttgttttca gaacaaatta tatatacgca tgaaaaaaca cttactttgc
      901 cttatcgctc ttttttattt ctttattagt acaacgaaca gccaagaaaa caccttgccc
      961 cgtgtaagca gtggtaccat tatacgtcat gtagatttta cttctaacta tatatctgct
     1021 cgcaatgtag atgtttggct acctgacaat tacagcccaa accaacgtta caaagtgctc
     1081 tatatgcacg atggacaaat gctttttgat gccaatacta cctggaacaa acaagaatgg
     1141 ggtgtggacg aaaccattac ccgccttata gcaaacgaca gtattctgag taccattgta
     1201 gtaggaatat ggaattctgg acgaacgcgt cattcggatt attttccaca aaaaccattc
     1261 gaacgcttgc ctgtatctac acaagactca ttaattttat tgggtagaag acaaaataat
     1321 gtttcattat ttgccgaacg tgtgcaatcg gataattacc tacgttttat tgtagaggaa
     1381 ctaaaacctt ttattgacaa aacgtatgct acgcttaccg aacctgaaca tacatttatt
     1441 gcaggctcta gcatgggcgg acttatttcg atgtatgctg tatgcgaata cccagaagtt
     1501 tttggtggtg cagcttgttt atctacacat tggattggta gttttcaaac agaaggaaac
     1561 ccaattcctg ctcaattttt tgcctattta aaagaaaatg taccctcacc agatacacac
     1621 aaattttact tcgattttgg aaccgaaaca ttagatgttc attacgaacc gtaccaacaa
     1681 caagttgatg caattatgca agccaaagga tacaattcta ccaattggat aacacgaaaa
     1741 tttgaaggaa aagatcattc agaaaatgcg tggcgtgaac gcttacacat tccgcttacg
     1801 tttttattac ccatacagtt caattaatta tcaacaaaaa aaacaatttt atgaaaacag
     1861 aaaataattc atcttgttgc acctctagtt cagagtactc gcaacaaatt ggcaaattag
     1921 cccttagatt acttgttgga ggatttatgc taacacatgg tttaccaaaa ttacaaaact
     1981 ttgagtacct aagtactgta tttcccgacc cattaggagt aggttcaagc ttatcgctcg
     2041 gcatgattgt atttgccgaa gtggtttgtt ccatcctatt aatgctgggc ttatttacac
     2101 gtctatccac tattcctctt attataggga tgggtgtagc agctttcttc attcatgcaa
     2161 acgacccatt tagtggtaaa gaaatggcat tgctatatat gggtatatat gttataatag
     2221 ctctcattgg agcaggaaaa tattcggcag actacttatt acgcaatcag tatgcaaaga
     2281 tttgttcatc gtattgtaaa ccaaaagctg aaaatacgaa gtgataaaac tgccaaaatg
     2341 acagaacaaa aaaatctttt ctaataaaaa aatgccaagc taaatactct ataagtttgg
     2401 catttttttt gatatttagt tagtgtaaaa caaatgttta accattaatt tataggagga
     2461 aaagttatgt cactagtaaa attctcaaac aattatccaa caatttttga tcgttttttc
     2521 gacaataatt tttacgattg gacaagtgaa aattattcaa cgacgaacac tactgtaccc
     2581 tcagcaaaca tcaaggaaag tgcagaaaaa tttgaaattc atattgctgc acctagtttt
     2641 gccaaagacg atttcaaaat tgaagttaac aacaacgtac taacagtatg tagtgaaaaa
     2701 aaagtagaaa acgaaaccaa agaaggagaa cgttttaccc gtaaagaatt tagttatcaa
     2761 tcgttttgtc gttcattcac tctacccatt agtgctgata ctgaaaaaat aagtgcgaat
     2821 tacgaaaatg gtattttagc tgttgatatt cctaaaaagg aagaagctaa gccaaaacca
     2881 agtaaaatta tagaaattaa gtagtatgta aaagggcatt tctggaaatt gaaatgccct
     2941 tttaatttgg taaaacttaa caacacaact atatttgttc aattacctgc aatccatgca
     3001 cctgcttcaa tctctctgtg atagtttcaa agttggataa tcgaatctgg acttttagcg
     3061 tgcaagaaat ttctaatgtt tgcgatatta tagtcaaatt attatcttta ataattttca
     3121 taacttcatt ca
//
LOCUS       DAOSMY010000180         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31265_length_3119_cov_4.762402, whole genome shotgun sequence.
ACCESSION   DAOSMY010000180 DAOSMY010000000
VERSION     DAOSMY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31265_length_3119_cov_4.762402"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..441
                     /locus_tag="PLD76_04785"
     CDS             127..441
                     /locus_tag="PLD76_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27351.1"
                     /translation="MEYRRPGVNRYFVEKELLPAYDSHTTNSPNTVLMGTGRKRRTRE
                     IQGWATRADYAAMVDDKNKAIKRTVTFDDGFTMDARIDKLEGNEQLGYNIVWYEAVFI
                     EG"
     gene            472..795
                     /locus_tag="PLD76_04790"
     CDS             472..795
                     /locus_tag="PLD76_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27352.1"
                     /translation="MNLNASEYNRNNIEQLGGEKDLIPYAGVITAQSSLLMSGRMRER
                     REISGWAEKTDFDSLETDYEAFTSRTVSFHDGTSITKAIIEELKGERRKGSTKVWYTA
                     KFLEV"
     gene            799..3069
                     /locus_tag="PLD76_04795"
     CDS             799..3069
                     /locus_tag="PLD76_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27353.1"
                     /translation="MRNIPAGITQLIKSKSMLGSDAPTGWVEFPTFGESYVLQVTSMS
                     VQREEAADAQRLVFSFPNVNPADPTDTGYYTPYRSTDFGKTANMWQNVLVPSTRVVAK
                     MGYGSNIATVFTGEIDEVRFSANPNDYTVSVDCRDLACLLIDKQIKLVLSGETEYYIE
                     YPLPAGVTKFWLDPGAVDIPDIADIVKDLCMRAGFAADDVIVEQTGIQLDPMWEKMSY
                     MDCINDLCTAAGFEFWVDEDGKANFFHQSDRKPNISGQSVVLNGTEWADLGHVSIVST
                     SDVVKDVTDVTTYSRTTDYEIDLVNGKLRRTAGSTIADGATVHVSYVFAGHIYREGED
                     IYGLNLTISRRNMYGTIRVAGDGEEASATVTSPLWDGSRVDLDKVLFADNQYLDELTK
                     VQKCANRLKSDMLNRYVSTDFVCVAHPWLQVGDCVQVIESSSTISEIYKVTSISFDLN
                     PDGFSSNIKAFHVGYTPLSATNWELLADAYDGTYGNEIKNMIVYNDELYAGCNGRLLK
                     YSSGAWVQVADKYLSGDTAVFIAEYNNKIYGGNGIYNPSGELLEWNGTNAWTLKAPQL
                     NGQSWPNFLIVYNNELYAATQTGSRLFKWNGTNAWVEVAGQAGGTENYMYTLCEYNGK
                     IYGGTFPNGQLYEWNGSNAWVLKAPQLNGQTQIKNLIVFNDALYGCTSGGRLFKWNDS
                     DAWIQIASVDATLHYMAVLGNQLYVVGSNGVLYRLYNSTLQQVCRYSAGIFTLMAYDG
                     ALYGGTINGQLIKCVP"
BASE COUNT         1022 a          538 c          753 g          806 t
ORIGIN      
        1 gtagcagcag acttagcagc aaacaaggat agatacagca agaacccaag tgccaataaa
       61 gcattcaaat aacggaaagg agagttggtt atggctgata cgtggggtgc aacaacacta
      121 aacataatgg aatacagaag acctggtgtt aacaggtact tcgtagagaa ggaactgtta
      181 cctgcctatg actcacatac taccaactct cccaataccg ttttgatggg tactgggaga
      241 aagcgtagga cacgtgaaat acaaggatgg gctaccagag cagattacgc agcaatggta
      301 gatgacaaaa acaaggcaat taagcgtaca gtgacattcg atgatggttt tacaatggat
      361 gctagaatag acaaacttga aggtaatgag cagcttggat ataacatagt gtggtatgaa
      421 gctgttttca ttgaagggta ggtgatataa atgactataa catggggtaa tttgaatttg
      481 aacgccagtg agtataatcg caataatata gaacaacttg gtggagaaaa agacttaatc
      541 ccatatgctg gtgtcatcac agcacaatcc agtttgttga tgtctggtag aatgagagaa
      601 cgaagggaaa tatcgggatg ggcagaaaag acggattttg actcattaga aacagattac
      661 gaagctttta ccagcagaac ggtatcattt catgatggca catcaattac aaaagcaatt
      721 atagaggaat tgaaaggcga acgcagaaag ggaagcacga aagtgtggta tactgcaaaa
      781 tttttggagg tgtaagcaat gagaaacata ccagcaggta taacacaact gattaaatca
      841 aagtcaatgc ttggtagtga tgcaccaaca ggctgggttg aatttccaac tttcggagaa
      901 agttatgtgc tccaagtaac atcaatgagt gtacaacgtg aagaagctgc cgacgctcaa
      961 aggttggttt tttcttttcc aaacgtaaat ccagcagacc ctactgatac aggttattat
     1021 acaccatata ggtcaactga ttttgggaag actgctaata tgtggcaaaa cgtattagtt
     1081 ccatctacac gagttgttgc taagatggga tatggcagca acatagcgac agtatttact
     1141 ggggaaattg atgaagtaag attctcagca aaccctaatg actatacggt tagtgttgat
     1201 tgtagagacc ttgcatgttt gctcatagac aaacaaataa agctagtttt aagtggcgaa
     1261 actgaatact acattgaata cccactacca gcaggtgtaa ccaagttctg gcttgaccct
     1321 ggagcagttg atataccaga tatagccgat atcgtgaagg acttgtgtat gagggctggt
     1381 ttcgctgcag acgatgtcat tgtagagcag acaggtatcc agcttgaccc aatgtgggag
     1441 aagatgagtt acatggattg catcaacgac ctgtgtaccg cagcagggtt cgagttctgg
     1501 gttgatgaag atggtaaagc caacttcttc catcagtcgg atagaaaacc aaacataagc
     1561 ggacagtcag ttgttctcaa tggtactgaa tgggctgacc ttggacacgt atccatcgta
     1621 agcacgtcag acgtggttaa ggatgttacg gacgttacga catacagtag gactaccgac
     1681 tacgaaattg acctcgtaaa tggcaaatta agacgaacag caggtagtac aattgcagat
     1741 ggtgctacgg ttcatgtaag ctacgtattc gctggacata tctaccgaga aggcgaggac
     1801 atctatggtt tgaacctgac aatcagcaga cgcaacatgt acggtacaat aagagtagct
     1861 ggtgacggtg aagaagcttc agcaaccgtt acatcacctc tgtgggatgg ttcgagggta
     1921 gacttggata aggttttgtt tgcagacaat caataccttg atgaacttac caaagtgcag
     1981 aaatgtgcca accgcctgaa gagtgacatg ttgaacagat atgtcagcac ggactttgta
     2041 tgtgtggctc atccatggtt gcaagtaggt gattgtgtac aagtcatcga gtcaagcagc
     2101 acgatatctg agatatacaa ggtaacatcc ataagttttg acttgaaccc tgacggtttt
     2161 tcaagcaaca tcaaagcttt tcatgttggg tatactccat tatctgctac aaattgggaa
     2221 ctcttggctg atgcttacga tggtacttac ggcaatgaaa taaaaaacat gatagtttac
     2281 aatgacgaac tatatgccgg atgtaacggt agactactta agtattcaag tggtgcgtgg
     2341 gttcaagtag ctgataaata tttatctggt gatactgctg tgttcattgc ggaatataac
     2401 aataagatat atggcggaaa cggaatatac aacccaagcg gcgaactgct tgagtggaac
     2461 ggtactaatg catggacgtt aaaagcacca caactaaacg gccaatcatg gcctaatttt
     2521 ttgatagtgt acaataacga actatatgcc gctacccaga caggcagcag gctatttaaa
     2581 tggaatggaa caaatgcatg ggttgaggta gctggtcaag ctggaggaac agaaaattat
     2641 atgtatactc tttgtgagta caatggaaaa atttatggag gaacttttcc gaacggacaa
     2701 ttatatgaat ggaatggctc aaatgcatgg gtactgaaag caccacaact caatggtcaa
     2761 acacaaataa aaaatcttat agttttcaac gatgctctct atgggtgtac atcgggaggt
     2821 agattattca aatggaatga ttctgacgct tggattcaaa tagcttcagt tgatgcgaca
     2881 ttgcattata tggcagtctt aggaaatcaa ctttatgttg ttggttcaaa tggagtcttg
     2941 tatagactat acaattctac attgcaacaa gtttgcagat atagtgctgg tatttttact
     3001 ttaatggctt acgatggagc gttatatggt ggaacaatca atggacaatt aataaaatgt
     3061 gtgccgtaag cagaaaaggg ggtagatgga catggctaac aatagaacag ctgctggtg
//
LOCUS       DAOSMY010000181         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31296_length_3117_cov_2.842913, whole genome shotgun sequence.
ACCESSION   DAOSMY010000181 DAOSMY010000000
VERSION     DAOSMY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31296_length_3117_cov_2.842913"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLD76_04800"
     CDS             <1..296
                     /locus_tag="PLD76_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU27354.1"
                     /translation="IMLLLPMLGVGLAWSSILSMPYAILTGSLPSHKMGTYMGIFNFF
                     IVIPQIMAATILGFFVTKIAGNHAIYALLLGGVSFLIAAVMTLFVKDEDEKNM"
     gene            328..2721
                     /locus_tag="PLD76_04805"
     CDS             328..2721
                     /locus_tag="PLD76_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU27355.1"
                     /translation="MKKLLLLVGIIALLVACSARGMLKLPVKSENNMRLNSPLVLVKG
                     SNFFLTQDYVIDITTIDSITADAKNGISVSLSANKDSAYINATDMPQLVNISLWSKGV
                     PYGVLAKKTNKIDYVFTYDATGDKGTRYQIAGQMNDWFPAGHPDLVKNEKGLYEVTLN
                     LSPGAYLYQMVIDGDWNHDKNNPDKVDNNYGKFNSILHVKGTQEKAPKLLTQSFDENN
                     IVLSVDNAAEQVYVYWQNYLLPNDLFVKNEGQRLIVNLPKEAIETDRSFLRIFTANEY
                     GISNDVLIPLQKGKVLNNVADITRFDKHAEAIYFLLVDRFKDGNEANNFPMNRPDVNP
                     KVDYQGGDIAGITQKIKDGYFQRLGFTTIWVSPIVQNPMEPWGYWANPETKFSGYHGY
                     WPISSSKIDFRFGTDEEMHELVKTAHENNINIIVDYVANHVHKNHPLYQRDSTIATPL
                     YLPDGTKNTERWNEHRLTTWFDEFMPSIDFSRQENIDMMVDSALYWVKKFDLDGLRHD
                     ACKHVNEEFWRTLTHRMKTEVEIPRGKLTYQIGESYGSPQLISSYVNTGMLDAQFDFN
                     VFDDAASVFAFNNEDFVRVSNGLKTSLSTYGHHNLMGYISGNHDRARFISYASGDVKP
                     GENAKLAGWTRKIELTDSTAYDKLIQLNAFNLTIPGVPVLYYGDEIGDPGANDPDCRR
                     FMRFGEQLVPAEERVLNTVAQLGQLRKNNLALIYGNFIELHLERDSWAYARNYFNEVA
                     IVVFNRQTTPKTIEIELPTILQNKQYAANFGNNFTVNANKLSITVAGNGFEVLTLKK"
BASE COUNT         1065 a          545 c          595 g          912 t
ORIGIN      
        1 ctattatgct gttattaccc atgttgggtg tgggattggc ttggtccagt attttatcca
       61 tgccatacgc tatcttaaca gggtcattgc catcgcacaa aatgggaaca tacatgggaa
      121 tatttaactt ttttattgta attccacaaa ttatggctgc tactatttta ggattttttg
      181 ttacaaaaat tgcaggtaac catgctattt atgccttact actaggtggc gtttcttttt
      241 tgatagcagc cgtgatgact ctttttgtaa aagatgaaga tgaaaaaaac atgtaactta
      301 tatatatttc aataaaacac tacaaacatg aaaaaattat tgcttttagt cggtatcata
      361 gcattattag tggcttgttc agcacgcggt atgcttaaac ttccagtaaa atcagagaac
      421 aatatgcggt taaactctcc gctggtgttg gtaaaaggaa gtaacttttt tttaactcaa
      481 gactatgtga tagatattac aacaattgat tccataacag cagatgctaa aaatggcatt
      541 agcgttagct tgtcagccaa caaagattca gcgtatatca acgcaacaga catgcctcaa
      601 ttggtaaata tttcgctatg gagtaaagga gttccttatg gtgtattagc caaaaaaaca
      661 aataaaattg attatgtatt tacttatgat gcaactggcg ataaaggtac acgttatcaa
      721 attgcaggac aaatgaatga ttggtttcct gctggtcatc cagatttagt aaaaaacgaa
      781 aaaggattgt acgaagttac tttaaacttg agtcctggtg cctatttgta ccaaatggta
      841 attgatggag actggaacca cgataaaaac aatccagata aggtagataa caattatgga
      901 aagtttaatt ctatcttaca tgtgaaaggt acccaagaaa aagcacctaa acttcttact
      961 caatcttttg atgaaaacaa tattgtattg tctgtagata atgccgcaga gcaagtatat
     1021 gtatactggc aaaattatct tctaccaaac gatttatttg taaaaaatga gggacaacga
     1081 ctcattgtaa acctcccaaa agaagctata gaaacagaca gatcattttt acgcatattt
     1141 acagctaacg aatatggtat atcaaacgat gtattaattc ctctacaaaa aggcaaagta
     1201 ctaaataatg tggcggacat tacacgtttt gacaaacatg ctgaagctat ttactttttg
     1261 ttagttgacc gttttaaaga tggtaacgaa gcaaacaatt tccctatgaa tcgtccagat
     1321 gtaaatccta aagtggatta ccaagggggc gatattgcgg gtataacaca aaaaattaaa
     1381 gacggttatt tccaacgttt aggatttacc accatttggg tatctcctat cgtgcaaaat
     1441 ccaatggagc catggggtta ttgggcaaat ccagaaacta aattttcggg ctatcatggc
     1501 tattggccca tctcttcgtc taaaattgat tttcgttttg gtacagatga agaaatgcac
     1561 gaattggtta aaacagctca cgaaaacaac ataaatataa ttgtggatta cgtggctaac
     1621 catgttcata aaaaccaccc actataccaa cgcgattcaa ctattgcaac gcctctctat
     1681 ttacctgatg gcacaaaaaa cacagaaaga tggaatgaac atcgtttgac aacatggttt
     1741 gacgagttta tgccaagtat tgatttttca cgccaagaaa acattgatat gatggtggat
     1801 tcagcacttt attgggtgaa aaaatttgac cttgatggtt tacgacacga tgcgtgcaaa
     1861 cacgtaaacg aagaattttg gcgtacactt acacacagaa tgaaaacgga agtagaaatt
     1921 ccaagaggga aactaaccta tcaaattggg gaatcgtatg gtagtcctca actcattagc
     1981 agctacgtaa atacagggat gttagatgca caatttgact ttaacgtttt tgatgatgca
     2041 gcatcggtat ttgcctttaa caacgaagat tttgtacgtg tgagcaatgg attaaaaacg
     2101 agtttaagca cctacggaca ccacaattta atggggtata tttcgggcaa ccacgacaga
     2161 gcacgtttta tttcgtatgc ttcgggagat gtaaaaccgg gagaaaatgc gaaactagct
     2221 ggatggacac gcaaaattga gttaacagat tctactgcct atgataaatt gattcagcta
     2281 aatgccttta accttactat tccaggtgta ccggtattgt attatggcga tgaaataggc
     2341 gacccaggtg caaacgaccc agattgtaga cgatttatgc gttttggcga acaattagtg
     2401 ccagcagaag agcgtgtatt aaacactgtg gctcaattag gtcagctacg taaaaacaac
     2461 ctagcactta tatatggtaa ttttattgaa ttgcatcttg aaagagattc gtgggcatat
     2521 gcacgtaatt atttcaacga agtagctatc gtagtattta atcgccaaac aacgcctaaa
     2581 accattgaaa ttgaattacc aacaattttg caaaataaac aatatgcggc taattttgga
     2641 aacaatttta ctgtaaacgc taacaaactc agcattactg tggcaggtaa tggatttgag
     2701 gtgttgacct tgaagaaata aattccaaaa aaataattct gaatagaggt tgctttatac
     2761 aaggcagcct ctatttttta gtgcaaaaac cttatgcaaa cttacatcgc tgtgtactat
     2821 tctcacacga ataacgcacg aaaatacacg aaaaaaaaaa catattactc tgtgtaagat
     2881 gtctcacacg aagaacacga agaagacacg aagttacacg aagaaaaata tatattactc
     2941 tgtgcccctc tgtgaaacct cggtggtact ctgtgaaaga atctctctca cgaagatatc
     3001 tattccgctc cgatggagct aattggtact gtactgtata ttttctatta tgatgtagcc
     3061 cctacggggc atcagagata gttactattt gagaaattga caaatagtaa gcagttg
//
LOCUS       DAOSMY010000182         3110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31379_length_3110_cov_3.486088, whole genome shotgun sequence.
ACCESSION   DAOSMY010000182 DAOSMY010000000
VERSION     DAOSMY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31379_length_3110_cov_3.486088"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..2263
                     /locus_tag="PLD76_04810"
     CDS             56..2263
                     /locus_tag="PLD76_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU27356.1"
                     /translation="MKQFLVVILAFFSIGVGTSYAQIQSGGFSPLPFTYSNEQNTIVG
                     KRNTQPLFYKIPYNGEQDNKEEKTSRFVVGKSFAVNYSPLNAGQQFTTEDGTTIWRIG
                     FYSPNAASISVVFDEFEIPEGASIFVYNPSQTNVAGAFTSINNNSNKSLPIAPFPSDS
                     IIIEYQQPATVTFNGKLRIGRVTHNTIDFINRSTGSFNSAEKCSPHAAFVEGVSNIKQ
                     ATCLLYVTDNSESWLGSGVMMNNSEGKPYLLTAAHNYEGTNLEQTTIFYFNYEVPKVD
                     SLIRGSQEFTLGGSVLKAKAYDIDFALVELNQMPPADYRPYLAGWNRANSDLAPMVCV
                     QHPQGDVKRVSTDNDNPLAVTYPYNDLLETKLADAHWKITRWEKGTTEPGSSGSGLFD
                     NNYRIIGGLTGGNSTCNSPVEDYFFRLNKAWSYYSSESKQLKRWLDPTNTNVMYMDGR
                     NPYEATPCIRYSNVQSTDVMGKVSVGNGKTGRLSGHNELQYTEFAEKFVTDTNNYLYG
                     VYIVPFIGKYNVNTPVNVHVYNGTSSPETLLTTVLFNPKTLAYQATSQTFSQTTKTQF
                     LNRENYVRFPNPVLVDEEFFVSYSITYQPVDSFAVYMVNNRAIDALNTAYYKDNNTWK
                     SFTEHPDKPMASSLWIEPVFQEANESLIFYPHDEDANAVLFPNPCDSEIQVFRKETNT
                     AQFSLFSIQGTLLFTISSIENPLTINTSGLQQGIYFLQITRDDTSNTYKLIKK"
     gene            2266..2991
                     /locus_tag="PLD76_04815"
     CDS             2266..2991
                     /locus_tag="PLD76_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HOU27357.1"
                     /translation="MLAKTRAIVIHTTKFNDKDLILHALTEEFGMVSYMIYNPWSKQA
                     KVKASYLQTFSLLEIDVEHQLKKDIQRIHDAKILFVGNSIHTNPYKQSIVLFLAEMVR
                     KTLSETEKDVHLFAFVYHSLCVLDEVTEKYNSFHLIFLMRLTQFLGFYPNSTVENNDV
                     YFDMLNGIFTAQPPLHGHYLEGENCKHFQALTHISYTDMPAWNISRVDKLTLLSTMVR
                     YYQLHIPTIQRIKSLDILHSLFV"
BASE COUNT         1062 a          591 c          543 g          914 t
ORIGIN      
        1 aatgaatcca ttttagtatt gcaaaaatct gctagcaagc attaaaattt tgacaatgaa
       61 acagttttta gtcgtaatat tggcgttttt ttctattggc gttggcacaa gctacgctca
      121 gatacaaagt ggaggatttt cgccactacc ttttacgtat tcaaacgaac aaaataccat
      181 tgttggaaag agaaatacgc agccgttatt ttacaaaatc ccctataatg gagaacaaga
      241 caataaggaa gaaaaaacat ctcgctttgt tgttggcaaa tcgtttgctg taaattattc
      301 tccgttaaac gctggacaac aatttacaac cgaagatggc acaacaatct ggcgtattgg
      361 gttctactct ccaaatgcag cctctattag cgtagtattt gatgagtttg aaatacccga
      421 aggggcttcg atttttgttt acaatccatc tcaaaccaat gtagctggtg catttaccag
      481 tataaataac aacagtaaca agagccttcc tattgcacca tttccgtctg atagtattat
      541 tatagaatac caacagcctg ccacagttac ctttaacggc aaactacgta ttggcagagt
      601 aacacacaat acaatagatt ttattaaccg aagtactgga tcgtttaata gtgctgagaa
      661 atgcagtccg catgcagcat ttgttgaagg agtaagcaac atcaaacaag ccacatgttt
      721 gctgtatgta accgacaatt ccgaatcgtg gttaggctct ggcgtaatga tgaacaatag
      781 cgaggggaaa ccttatttgc ttacagcagc acataattac gaagggacta atttagaaca
      841 aacaaccatt ttttatttta attatgaggt accaaaagtt gactcactca ttcgtggttc
      901 gcaagaattt actttaggag gaagtgtatt aaaagcgaaa gcgtatgata ttgattttgc
      961 actggttgag ctaaaccaaa tgccaccagc agattaccgt ccttatttag ctggatggaa
     1021 tagagctaac tctgatttag cccctatggt atgcgtacag catccacaag gagatgttaa
     1081 acgagtgtca accgacaatg acaatcctct tgcagttact tacccctaca atgatttgct
     1141 agaaaccaaa ttagcagatg ctcactggaa aataactaga tgggaaaaag gaacgacaga
     1201 accgggctct tctggttcgg gtttgtttga caacaactac cgtattattg gcggactaac
     1261 aggaggtaat tcaacctgta atagtcccgt agaagattat ttctttcgtt taaacaaagc
     1321 ttggagttat tatagcagcg aaagtaaaca attaaaacgc tggttagacc ctaccaatac
     1381 caatgttatg tatatggatg gaaggaatcc atacgaagca accccttgca tacgttattc
     1441 aaacgtacaa agtacagacg taatgggcaa ggtctctgta ggaaatggta aaactggacg
     1501 tttgtcggga cataatgaac tgcaatatac agaatttgca gagaaatttg taacagatac
     1561 caacaactat ctatatggcg tgtacattgt accatttatt ggcaaataca atgtgaatac
     1621 tcctgtaaac gtacatgttt ataatggaac atcaagcccc gaaacactat taacgacagt
     1681 tctctttaac cctaaaacac tagcatatca agctacttcg caaacatttt cacaaactac
     1741 taaaactcaa tttttaaata gagaaaatta tgtacgcttt ccaaatcctg ttttagttga
     1801 tgaagaattt tttgtttcct attccattac ttatcaacca gtagattcgt ttgctgtgta
     1861 tatggtaaat aacagagcga tagatgcact caataccgcc tattacaagg acaataatac
     1921 ctggaaatcg tttacggaac atccagacaa accaatggca agttcgctgt ggattgaacc
     1981 tgtatttcaa gaagcaaacg aaagtttaat cttttaccca cacgatgaag acgctaatgc
     2041 agttttattt cccaatccat gcgattctga aattcaagta tttagaaaag agacaaatac
     2101 agcccaattc tctctgtttt cgatacaagg gacactcctc tttacaataa gtagcatcga
     2161 aaacccactt accatcaata cgagtgggtt acaacaaggc atctactttt tgcaaataac
     2221 aagagacgat acatcaaaca cctataaatt gataaaaaaa tagctatgct tgctaaaaca
     2281 cgggctattg ttattcacac tacaaagttc aacgataaag atcttattct gcatgcacta
     2341 acagaagaat ttggcatggt atcgtacatg atttataatc catggagcaa acaagccaaa
     2401 gtaaaagcat cgtacctaca aacatttagt ctgctagaaa ttgatgtaga acatcaactt
     2461 aaaaaagata ttcagcgtat acatgatgcc aaaatactat tcgtaggcaa tagcatacat
     2521 accaatcctt acaagcaaag tattgttctc tttttagccg aaatggtacg taaaacatta
     2581 tcagaaacag aaaaagacgt tcacttattt gcttttgtct atcattcgtt gtgtgtatta
     2641 gatgaagtaa cagaaaaata caattctttt caccttattt tcttaatgcg attaactcaa
     2701 ttcctaggtt tttacccaaa tagtacagta gaaaataatg atgtgtattt tgatatgcta
     2761 aacggaatat ttactgcaca gccacctttg catggtcatt atttagaagg agaaaactgt
     2821 aagcattttc aggcacttac gcacataagc tatactgaca tgcctgcttg gaatatatcg
     2881 cgtgtagata aattgacatt actatcaact atggtacgtt actatcaatt gcatataccg
     2941 accatacaac ggataaaatc cttggatatt ctccattctt tgtttgtatg atgtaaaaaa
     3001 atagtttgtt tcttactgga aaaaatagta cttttgctca tcaaattttt gcctctgtag
     3061 ttcaatggat agaacaaaag tttcctaaac tttagattcg ggttcgattc
//
LOCUS       DAOSMY010000183         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31597_length_3093_cov_4.526004, whole genome shotgun sequence.
ACCESSION   DAOSMY010000183 DAOSMY010000000
VERSION     DAOSMY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31597_length_3093_cov_4.526004"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..599)
                     /locus_tag="PLD76_04820"
     CDS             complement(117..599)
                     /locus_tag="PLD76_04820"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU27358.1"
                     /translation="MAKNCTRIFLVGFMGTGKTTFGKKLSQKIHFSFIDTDLYIENNF
                     HKTINEIFAEKGEEGFRTIETKILAEIAEIENVVIATGGGTPCFNNNMALMNSKGLTI
                     YLETPTPDIAQRLITGKTKRPLLKDKTSEEIVEFIEEILPKRSTYYKKADLTINVVEN
                     "
     gene            complement(596..2029)
                     /gene="cls"
                     /locus_tag="PLD76_04825"
     CDS             complement(596..2029)
                     /gene="cls"
                     /locus_tag="PLD76_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOU27359.1"
                     /translation="MEFLSDILILIFLATYVYTVVSSIVVVLLENKHPVRTSAWIMVL
                     IFVPFIGLLLYVVVGRSYHRQGNISKKLKNKILSFGTRQSIKEICAENNICSKHERLI
                     HLLRNSTDASIFTHNSVNIYTDGKQLFDDILSAMDNATAHIHAEYYAVETDETGNRFK
                     DMLIKKSKQGLEVRLIYDDVGSWRLKRSTIQEMKNAGVILQTFFELRFPYFTNKLNYR
                     NHRKILIIDGKIGFLGGVNIADRYTKGLPWGIWRDTHIKIQGNAVDGLQQLFLNDWLY
                     ITKTAIHHKKYFAIHDQYNKLPIQIVGSGPDSMWEGIMQGFCAAIQNASKYVYIQTPY
                     FLPNDSISNALESAALSGIDVRIMIPSRSDTRISLEASLSFVKTMLAAGVRVYQYQAG
                     FIHAKSIAIDNELAIIGSANMDFRSFEQNFEVSAFMYDSDKSMELRNIFLHDMKQCKR
                     LQYTQWKHRPITQKLIQSSARLFSPLL"
     gene            complement(2029..>3093)
                     /gene="ribD"
                     /locus_tag="PLD76_04830"
     CDS             complement(2029..>3093)
                     /gene="ribD"
                     /locus_tag="PLD76_04830"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOU27360.1"
                     /translation="RVFWTSFKPNKMSIHETYMHRCLQLASLGKGRVAPNPMVGCVIV
                     HHDVIIGEGYHRTYGEAHAEVNAIESVKNKALLKESTLYVSLEPCSHYGKTPPCADLI
                     IQYKIPRVYIGTKDPNPKVSGNGIARLETAGVAVECGILAAQAMEINKYFFTFQLKNR
                     PYITLKWAKSADGYIDALQPKPIQLSNEITKTLVHKLRSENMAILVGTNTALKDNPSL
                     RTRRWFGKNPVRIGIDKNLRIPKNYLLYNGQTTTLLFNSIKQEEGNPCMIQIDFTKDI
                     IPQLLHELYKRNIHSLIVEGGKILLDSFINTGLFDEIHEETADVLLYEGTCAPQFSES
                     ILCSMEIVGKNTCATYVKKE"
BASE COUNT          895 a          557 c          514 g         1127 t
ORIGIN      
        1 ttagagctta cagatgatga attagcttct attgccactt ccattagaac atttcttgcc
       61 taaagggagc taacatataa ttgacagagg aggagagttt ataagacttt cctcttctaa
      121 ttttcaacca cattaatagt gagatcggct tttttgtaat acgtacttct tttaggtagt
      181 atttcttcta taaattccac tatttcttca gaagttttat cttttaataa aggtcgtttt
      241 gtttttcctg taatcaaacg ttgtgcaata tcaggagtgg gtgtttctaa atagatggtt
      301 aatcctttac tattcatcaa tgccatatta ttgttaaagc acggagtacc tcctcctgtt
      361 gcaattacta cgttttcaat ttccgcaatt tctgctaata ttttcgtctc aattgtgcga
      421 aacccttctt ctcctttttc agcaaaaatt tcattaatgg ttttatgaaa attattttcg
      481 atatataaat ctgtatcgat aaacgaaaaa tgaatttttt gggatagttt tttcccaaat
      541 gttgtttttc cagtgcccat aaagccaact aaaaaaattc tggtacagtt ttttgctata
      601 agagcgggct gaataatcga gctgatgatt gtatcaactt ttgagttatt gggcgatgtt
      661 tccattgtgt atattgtaaa cgtttacatt gtttcatatc gtgtaaaaag atattacgta
      721 gttccatgct tttgtctgag tcatacataa atgcacttac ttcgaagttt tgctcaaaac
      781 tcctaaaatc catgtttgca cttccgataa ttgcaagttc attgtcaata gcaatgcttt
      841 ttgcgtgaat aaaacctgct tgatattgat atacacgcac tccagcagct agcatggttt
      901 taacgaaaga taaagaagct tctaatgata tacgggtgtc agaacgactt ggaatcataa
      961 ttctaacgtc tattccagat aatgctgcag attctaatgc attggatatg ctgtcattag
     1021 gtaaaaaata aggtgtttgt atatacacat atttggaggc gttttgtata gcggcacaaa
     1081 atccttgcat tatcccttcc cacatcgaat ctggaccact tcctactatt tgaatgggta
     1141 gtttattgta ttgatcgtgt atagcaaaat attttttgtg atgtattgct gttttagtaa
     1201 tatataacca gtcattcaga aataattgtt gtaatccatc tactgcattt ccttgtattt
     1261 tgatatgcgt atccctccat attccccaag gcaagccttt tgtatatcta tctgcaatat
     1321 ttacacctcc taaaaagcca attttcccat caatgattag tatctttcgg tggttacgat
     1381 aatttaattt atttgtaaaa taggggaatc gtaattcgaa aaatgtttgc aatatcacgc
     1441 ctgcattttt catctcttgt atggtggatc gttttaatcg ccagctacca acatcgtcat
     1501 atattaatcg cacttccagt ccttgtttcg attttttgat aagcatatct ttaaagcgat
     1561 tgcctgtttc atcggtttca acggcataat attcggcatg gatatgtgca gtagcgttat
     1621 ccatagcact gagtatatcg tcaaataatt gtttcccatc ggtataaata ttgacactat
     1681 tatgtgtaaa tatggaagca tctgtactgt ttctcaataa atgaattaat ctttcgtgtt
     1741 tactacaaat gttattttca gcacaaattt ctttaataga ttgccttgtc ccaaaagata
     1801 aaattttgtt ttttagcttt tttgatatat taccttgtct atgataactt cttcccacaa
     1861 caacatataa taatagtcct ataaatggga caaaaataag taccataatc cacgccgaag
     1921 tacgaacagg atgcttgttt tctaacaata caaccacaat gcttgatacc accgtataca
     1981 catacgtagc aaggaatatc aatatgagta tgtcggataa aaattccatt attctttttt
     2041 tacgtaagtt gcacatgtat ttttgccaac tatttccata gaacataaaa ttgattcgga
     2101 aaattgtgga gcacaagtac cttcgtacaa taaaacatct gcggtttctt cgtgaatttc
     2161 atcaaacaat cctgtgttaa taaaggagtc taataaaatt ttaccgccct ctacaatcaa
     2221 cgaatggatg ttccgtttat acaattcgtg taacagttgg ggtataatat ctttggtaaa
     2281 gtctatttgt atcatacacg ggttaccttc ttcttgcttg attgagttaa aaagtaaggt
     2341 tgttgtttgc ccattgtaaa gcaagtaatt tttaggaata cgtaaatttt tatctatacc
     2401 tatacgtact gggttttttc caaaccaccg cctagttcgt agcgatggat tgtcttttaa
     2461 tgctgtattt gtgccaacca atatcgccat attttcagaa cgtaatttat gaaccagtgt
     2521 ttttgttatt tcatttgaca gttgtattgg ctttggttgt agggcatcaa tatatccatc
     2581 ggcacttttt gcccatttca acgtaatata tgggcggttt tttagttgaa atgtaaaaaa
     2641 atatttgttg atttccattg cctgtgctgc taatatccca cattccacag caacacctgc
     2701 tgtctccaaa cgtgcaatac cattgcctga tacttttggg ttagggtctt tcgttccaat
     2761 atatacacga ggaattttat actggatgat aaggtctgca caaggcggag ttttaccgta
     2821 atgcgaacag ggttctaagc taacataaag tgttgattct tttaataatg ccttattctt
     2881 taccgattca atggcgttta cttctgcatg tgcttctcca tacgtacgat ggtatccttc
     2941 tcctataatt acatcgtgat gtacaataac acatccaacc attggattgg gagctactct
     3001 gcctttgcct aaggaggcta attgcaagca acggtgcata tatgtttcgt gaatactcat
     3061 tttatttggc ttgaatgatg tccagaaaac tct
//
LOCUS       DAOSMY010000184         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31771_length_3078_cov_3.977506, whole genome shotgun sequence.
ACCESSION   DAOSMY010000184 DAOSMY010000000
VERSION     DAOSMY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31771_length_3078_cov_3.977506"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="PLD76_04835"
     CDS             <1..1312
                     /locus_tag="PLD76_04835"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOU27361.1"
                     /translation="YGFLGGPGGLVDHKYIELTSEIIDEYRNTGGFDIIGSGRTKLEE
                     TAQYDKGVEICNKLGIQALIVIGGDDSNTNACVLAEYYLQKNTGIQVIGCPKTIDGDL
                     KNDMIETSFGFDTACKVYSELIGNIMRDANSAKKYWHFIKLMGRSASHIGLECALQTQ
                     PNICIISEEVEAKKQTLKEVVDSIAKVVATRAAKGNNFGIALIPEGLIEFIPEMKVLI
                     SELNDLLAHESDVQKALNGLSKASAEVFCSLPENIAKQLTLDRDPHGNVQVSLIETEK
                     LLIEMVGKRLKEMKAEGQYTGKFAGLGHFFGYEGRCAAPSNFDADYCYSLGYNAAQLI
                     ATGKTGYMSSIRNTTAPAEQWIAGGVPVTMMMNMERRHGAMKPVIQKALVRLDGAPFK
                     TFAANRDKWATDYCYVYPGPIQYFGPTEVCDQPTKTLQLEQAGK"
     gene            1422..1497
                     /locus_tag="PLD76_04840"
     tRNA            1422..1497
                     /locus_tag="PLD76_04840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1458..1460,aa:Pro,seq:ggg)
     gene            complement(1607..2830)
                     /locus_tag="PLD76_04845"
     CDS             complement(1607..2830)
                     /locus_tag="PLD76_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU27362.1"
                     /translation="MKVRNWTKWTPLFLSNFMGVLNDNFLKYSIIFMAISWSLPTWFT
                     QSQLIALVSFALVIPYVFLSPLGGMLATRYSKRKVFLFFKLLEIPIMAIACVAFYYQY
                     VFLAIFSVLLMGIQSSLYSPAKYSLIRDIGGEKGVSFGTGVFEMMAFLGILAGTVLAS
                     YMADRYSYVWVLICFLGFAIIGYLVTSQIRAKEIAAPQELKIALHPFAFIIQSYHKSR
                     AFPMLNAAVFGSAFFWFLGSILQMNIVIHSTVVYGLSNTETGVIVSIVAIALGVGCWI
                     AGKILTNTNIYVLLLVGISGIAISSFVLGIYCYPLRTFLALLVILSLCGGLFQVTCLS
                     LIQKTAKEISLADIFAYVNLLTFICILIGAGLFSLINWMSGDNSFYIFFLLSAISFLL
                     TFFYFFKVTSIRKLH"
BASE COUNT         1113 a          556 c          542 g          867 t
ORIGIN      
        1 gtatggattt ttgggcggac ctggtggttt agttgaccat aaatatattg aacttacaag
       61 cgagattatt gatgaatacc gcaatactgg aggttttgat attattggtt ctggtcgcac
      121 aaaattagaa gaaacagcac aatacgataa aggcgtagaa atttgcaaca aattaggcat
      181 tcaagcactt attgttattg gtggagatga ttcaaacaca aatgcgtgtg tattggcaga
      241 atattatttg caaaaaaata caggtattca agtaattggt tgcccaaaaa ccattgatgg
      301 tgaccttaaa aatgatatga tagaaacatc atttggattt gatactgctt gtaaagttta
      361 ttcagaactt atcggcaata tcatgcgtga tgcgaattct gcaaaaaaat actggcattt
      421 tattaaatta atgggacgtt cagcttcgca tatcggactt gaatgtgctt tacagacaca
      481 accaaacatt tgcatcattt cggaagaagt agaagcaaaa aaacaaacgc taaaagaagt
      541 ggttgatagc attgctaagg tggtagctac tcgtgctgcg aaaggtaata attttggtat
      601 tgcacttatt cctgaagggt taatagagtt tattcctgaa atgaaagttt taatttcgga
      661 attaaacgac ttgctagcac acgaatcgga tgttcaaaaa gcactcaacg gattaagcaa
      721 agcaagtgct gaagtattct gtagcttacc agaaaatatt gctaagcaat taacccttga
      781 tcgcgaccca catggcaacg tgcaagtatc attgattgaa acagaaaaat tgttgattga
      841 aatggtaggt aaacgattga aagagatgaa agccgaagga caatacacag gtaaatttgc
      901 tggcttagga catttctttg gttacgaagg acgttgtgca gctccatcaa actttgatgc
      961 tgactactgc tattcattag gatacaatgc agctcaattg attgcaacag gcaaaacagg
     1021 ctatatgtcg tctatccgca atactactgc ccctgcagaa caatggattg ctggtggtgt
     1081 acctgttact atgatgatga atatggaacg tcgtcatggt gcaatgaaac ctgtaattca
     1141 aaaagcattg gttcgtttag acggtgctcc atttaaaaca tttgcagcca atcgcgacaa
     1201 atgggctact gactattgct atgtatatcc tggtccaata caatattttg gtccaacaga
     1261 agtatgcgac caacctacaa aaaccttgca attggaacaa gcaggtaaat aaattacttc
     1321 attgaagaat aaaagcaact cgaacaaaaa gggttgcttt tttagttcat attttatttg
     1381 acaaacacaa aaaattacgt acatttgcat tcccaatagt tcggggtgta gctcagcccg
     1441 gtttagagta cacgtctggg gggcgtgtgg tcgcaagttc gaatcttgtc accccgacta
     1501 acaacaaagc aagactttcc acaagaaagt cttgctttgt tgtataggga catgatgttt
     1561 ccacaacaaa caactatttc aggtctttgc tttttattct atttattcaa tgcaactttc
     1621 gtatagacgt taccttaaaa aaataaaaaa acgtcaacaa aaaagatatc gcagataaaa
     1681 gaaaaaatat ataaaaacta ttatcacctg acatccaatt tattagagaa aataatccag
     1741 caccaattaa gatacatata aaagtaagca aattaacata agcaaaaata tccgctaacg
     1801 aaatttcctt agctgttttt tgaataagag acaaacacgt aacttgaaat aatccaccac
     1861 acagggaaag aatgacgagt aatgctaaaa atgtccgtaa tggatagcaa tatataccta
     1921 aaacaaagct actaattgct attccactaa tacctaccaa taatagtaca tatatattgg
     1981 tgttcgttaa aattttacca gctatccaac accccactcc caatgctatg gctactatgg
     2041 acacgatgac acccgtttct gtattagata atccgtatac aacagtacta tggatgacga
     2101 tattcatctg caatatacta cctaaaaacc agaaaaaagc cgatccaaac acggcagcat
     2161 ttaacatggg aaaagctcga gatttatgat acgattgaat aataaatgca aatggatgta
     2221 atgctatttt aagctcttga ggagcagcaa tttcttttgc tcttatttga gatgtaacta
     2281 gatagccaat aatagcaaaa cctaagaaac atatcaatac ccagacataa ctatacctat
     2341 cagccatgta agatgccaat actgtaccag ctaatattcc aagaaatgcc atcatttcga
     2401 atacacccgt tccaaacgat accccttttt ctcccccaat atcacgtatt aaactatatt
     2461 tggcaggaga atacaagcta ctttgaatcc ccattaacaa aacggaaaaa atagctaaaa
     2521 acacatattg ataataaaac gccacacaag ctattgccat aatggggatt tctagcaact
     2581 taaaaaaaag aaaaaccttg cgtttggaat agcgagttgc caacataccg cctagaggag
     2641 ataaaaaaac atagggtatt actaacgcga atgataccag ggctattaac tgagattgag
     2701 tgaaccaagt aggcaatgac caggatatcg ccataaaaat aatggagtat ttcagaaaat
     2761 tatcatttaa tacacccata aaattgctaa gaaacaaggg tgtccattta gtccaattac
     2821 gtactttcat ctgtcttggt tatcttacat tttaattttg aacaagacaa aagtacctat
     2881 atttttttat atgcaataaa aaaattagtt tctgatacaa gattgtttga agtatcgtat
     2941 cataaggatt acacaacaaa agccctgcgt atctatcagg tagttcgcag ggcttagtaa
     3001 agaaaaaaat tatcgcttaa caaaaaacat gactcatcat tctgttttta acaacattct
     3061 actactttca tgtagctt
//
LOCUS       DAOSMY010000185         3077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_31784_length_3077_cov_3.341496, whole genome shotgun sequence.
ACCESSION   DAOSMY010000185 DAOSMY010000000
VERSION     DAOSMY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31784_length_3077_cov_3.341496"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="rmuC"
                     /locus_tag="PLD76_04850"
     CDS             complement(<1..1290)
                     /gene="rmuC"
                     /locus_tag="PLD76_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOU27363.1"
                     /translation="MEWMWISIGLVSVLAVLWLIVKYMKQLMHNKLAEQEKALASSIA
                     VLEKEKALAQMQLADVQNVYQQTLQQLSKETEKYQQLSSQYAVLQTQNTHLSERLDTQ
                     KKELTELQQHFTKEFENIANRILKQNSSDFTDVNVKHLTDIVNPLKEKISLFEKKVED
                     AYDKELRDKLSLREEVRKLTELNTRISEEATNLTKALKGDVKKQGNWGEIVLERVLER
                     SGLRKGIEYTREEQIEGDEGQVYRPDVIVHLPDNKHIIVDSKVSLLAYENFVNANDDE
                     KDAYLKAHIVSLRNHIKGLGEKCYQNSPTLNSPDFVLMFLPIEGSFSVALQFDNELYS
                     FAWDRKIVVVSPTTLLATLRTIASIWKQENQTKNAQEIARLGGALYDKLVGCVEDLNK
                     VKLNIDRASASHNDAMKKLQLGAGNVFVTAEKMKQLGA"
     gene            complement(1303..1518)
                     /locus_tag="PLD76_04855"
     CDS             complement(1303..1518)
                     /locus_tag="PLD76_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27364.1"
                     /translation="MKKPSKRNDKNTQKSLLRKNRVTFVLNDKELNALEAYCKRFRVK
                     NKSRFVRTVLMSTVIERLEQSSPTLFD"
     gene            1616..1984
                     /locus_tag="PLD76_04860"
     CDS             1616..1984
                     /locus_tag="PLD76_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU27365.1"
                     /translation="MAIHNKLGEEGEKLATAYLQQKGYRIRHTNWRSGHLELDIVAES
                     HDTLYVIEVKTRSTAYFEHPKDAITNAKIKRIVHATEEYIHRFDIHKDIQFDIMALLP
                     NKHGTFDIEHIPDAFLAPLS"
     gene            1999..2352
                     /locus_tag="PLD76_04865"
     CDS             1999..2352
                     /locus_tag="PLD76_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOU27366.1"
                     /translation="MNIEEVRTYCLSKPYVTEDFPFDETVLVFRVAGKIFVLCALDAM
                     PPRINVKCDPQKAIELRELYNYVVPGFHMSKKHWNTIFIESVDNEQQLRTWIDESYEL
                     VIKRLTKKEQKQLNM"
     gene            2453..>3077
                     /locus_tag="PLD76_04870"
     CDS             2453..>3077
                     /locus_tag="PLD76_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOU27367.1"
                     /translation="MKLALIGYGKMGKEIEQIAISRGHEIVSRIDVDNQEDFKSDAFK
                     SADVAIEFTAPHVAYANYLKAFEAGVSIVSGTTGWLDKINEIKAICHDKQLAFFWASN
                     FSLGVNIFAAVNSYLAKIMNQYSEYNVSMTEVHHTQKLDAPSGTAITLAEGIIKELAR
                     INKWELETASTKDSLAIHAIREGNVPGIHTIRYESEVDSIAITHDAKS"
BASE COUNT          974 a          574 c          538 g          991 t
ORIGIN      
        1 tgcccctagc tgtttcattt tttcggcagt aacaaatacg tttcctgctc ctaattgcag
       61 ttttttcatg gcatcgttat gcgaagctga ggctctatca atatttaact tcaccttgtt
      121 taaatcttct acacaaccca ctaatttatc gtacaaagct ccacccaaac gtgcaatttc
      181 ttgggcattt ttggtttgat tttcttgttt ccagatggag gctatggtac gcaaagtagc
      241 caataaggta gtaggactta ccaccacaat ctttctatcc caagcaaagc tataaagctc
      301 attatcaaac tgtaacgcta ccgaaaaaga gccctcgata ggcaaaaaca ttaatacaaa
      361 atcaggagaa tttaatgttg gagaattttg ataacacttt tcgccaagtc cttttatatg
      421 attgcgaaga gaaactatat gagccttgag gtacgcatcc ttctcgtcgt cgtttgcatt
      481 cacaaaattt tcatacgcta gtaacgacac ctttgaatcc acaataatat gcttattatc
      541 aggcaaatgg acgataacgt caggtctata tacttgacct tcatcccctt caatttgttc
      601 ttcgcgtgta tattcaatac cttttcgcaa gcccgaccgc tccaataccc tttcgaggac
      661 aatttcgccc cagttacctt gcttttttac atcacctttt aaagctttcg tcagattagt
      721 ggcttcttcg ctgatacgcg tatttagctc agttaatttt cgtacttctt cacgcaaact
      781 tagcttgtct ctaagctcct tgtcgtaagc atcttccact ttcttttcga acaaagaaat
      841 tttttctttt aatggattga caatatcggt aaggtgtttt acatttacat ctgtaaaatc
      901 agaagaattt tgtttcaaaa tgcgattggc aatgttctcg aactctttgg taaaatgttg
      961 ttgcaattcg gtaagttctt ttttttgcgt gtctaatcgt tcggacaagt gcgtattttg
     1021 tgtttgcaac acagcatact gagaggatag ttgttggtat ttttcagttt ctttactaag
     1081 ttgttgtaag gtttgctgat acacattttg cacatcagcc agttgcattt gagccaaggc
     1141 tttctccttt tctaataccg caatagaact ggcaagagct ttttcttgct cggctaattt
     1201 attgtgcata agctgtttca tgtactttac aataagccat agcacagcaa gcacacttac
     1261 taacccaata cttatccaca tccattccat tgtatatctt ttttaatcaa acaaagttgg
     1321 actactttgt tctagtcgtt caataaccgt tgacattaaa acagtcctta caaaacgtga
     1381 tttatttttc accctaaaac gtttacaata tgcctccaac gcatttaatt ctttgtcgtt
     1441 taacacaaaa gtaactctat tttttctgag taatgatttt tgtgtatttt tgtcgttacg
     1501 tttgctaggt tttttcattc actagatttt ttctctatct accttgcaaa agtacaacgc
     1561 tttatataat taaaaaaatg cttttaagaa aaaacgtaat aaatttgtaa aataaatggc
     1621 gatacacaac aaactgggag aagaaggaga gaaattagct actgcatatt tgcaacaaaa
     1681 agggtatcgt atccgccata ccaattggcg tagtggacat ttagagttgg atattgtggc
     1741 tgaatcgcac gatacgcttt atgttattga agtaaaaaca cgcagtactg cctactttga
     1801 acatcctaaa gatgcgatta ccaatgccaa aataaaacgc attgtgcatg caacagaaga
     1861 atacattcat cggtttgata tccacaaaga catacaattt gatatcatgg ctctactgcc
     1921 taataagcat ggaacatttg atattgaaca tattccagat gcatttttag ccccactttc
     1981 ataaaaaaag cttagacaat gaacattgaa gaagtacgaa catattgttt gtcaaaacct
     2041 tatgttactg aggattttcc ttttgacgag accgtattgg tatttagagt tgctggaaaa
     2101 atatttgtat tgtgtgctct tgatgccatg cctcctcgca tcaatgtaaa atgtgaccct
     2161 caaaaagcca ttgagctgag agaattgtac aactatgtag taccagggtt tcacatgagc
     2221 aaaaaacatt ggaatacgat attcatagaa tctgttgaca atgagcaaca actccgcact
     2281 tggatagacg aatcgtacga attagtgatt aaaagattga caaaaaagga gcaaaaacaa
     2341 ctgaatatgt aattatttta ggaatggact ccaattttta tacattattt gtatatttgc
     2401 aagttatagt ttttttgcat aaaacaacca ataaaaacct aaacttataa ttatgaaatt
     2461 agccttgatt ggatatggta aaatgggaaa agaaattgaa caaattgcca tttctcgtgg
     2521 acacgaaata gtaagcagaa tagatgttga caaccaagaa gattttaagt cagacgcgtt
     2581 taaaagtgcc gatgtagcga tagaatttac ggcgcctcat gtggcttatg ccaattactt
     2641 aaaggcattt gaagcaggcg tttcgatagt ttcgggcact actggatggc ttgataaaat
     2701 taatgaaatt aaagcaattt gccacgacaa gcaactggca tttttttggg catccaattt
     2761 cagtttagga gttaatattt ttgctgcagt aaatagctat ttggcaaaaa ttatgaatca
     2821 atattctgaa tacaatgttt ctatgacaga agtacatcat acacaaaaat tagatgcacc
     2881 gagtggaact gccattacat tggctgaagg aatcattaag gagctagcac gtataaataa
     2941 atgggaatta gaaacggcat ctacaaaaga ttcgctggca atacatgcta ttagagaggg
     3001 aaatgtacct ggcatacaca caattaggta cgaatcggag gtagatagca ttgcaataac
     3061 gcacgatgct aaaagcc
//
LOCUS       DAOSMY010000186         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32187_length_3049_cov_2.394122, whole genome shotgun sequence.
ACCESSION   DAOSMY010000186 DAOSMY010000000
VERSION     DAOSMY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32187_length_3049_cov_2.394122"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLD76_04875"
     CDS             <1..792
                     /locus_tag="PLD76_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27368.1"
                     /translation="AIADYNSAIDVNPSYTKAYYNRGIVKMMLLDYQGAIIDYNRALE
                     LNSNDAETYNNRGICKESVNDYQGALIDYNKAIELNPSFSEAYNNRGVLYRKMNNYKA
                     AMADYDKLLVLTPKNAEAYYHRGIVKYYISDLSGSITDFTQAITFNSQLFEAYMYRGI
                     VKNMLNDFSGAILDYNEAIKLNPESAEAYYNRGVSKGRLQEHQSSIEDFTQAMNYKMN
                     YAEAIFNRGVSYLALAQKDAACADFSKASQLGFYKAFEVIAVFCK"
     gene            complement(796..1413)
                     /locus_tag="PLD76_04880"
     CDS             complement(796..1413)
                     /locus_tag="PLD76_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27369.1"
                     /translation="MNKPIISIISSIAGICTLLFIVLHLAVNLLLVLDAIANTQGYYY
                     NSMHYFMQHNSVAYTLEKTFALGFVLHILLANILAFNKIRNKQGSKDMIIMGFLLLIF
                     TVLHVLQFKVKRTIEYVELDGVLKPDIYQFISHFFSRNWLYSLLYMFSGILLGVHLQN
                     GFVSMIKKINIDKKYQKLISITIKITSYSIMIGFLLIPLYFLFFS"
     gene            complement(1481..>3049)
                     /locus_tag="PLD76_04885"
     CDS             complement(1481..>3049)
                     /locus_tag="PLD76_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU27370.1"
                     /translation="GIRGKLEWKMKNNWQSMFQISYDYTDQIAYPYAKFDTISKQIYP
                     INYNEQGAYRRKMLSSGFTLQKITDNYAFTSATGFQNITDRMDIDQDFTSDSIMVLQQ
                     NQHLKSITQEFVFRSNNSNNWQWVNGLFLFGASNTIDSPMTFQKGGIKMIQNFMDMAK
                     MFNPSMPTITILNEEMLVAGLFSKPSIGAALYHQSSYSFTNKFSVTAGIRLDYEKAFI
                     DYDSEATLQTSVKLPFPFVPAFRVDSTYAEKGFKSKDFIEVLPKVALHYKIDNQHRVY
                     TSLTKGYKAGGYNYSMFSDILQAQMQGNKDVTIDDKIYYKPEESWNIEVGSNGTFGNN
                     RFNYQTAIFYIHHYNQQLSTTTSNGSRMISNAEVSQSIGGELSVKTYVSKQIAGYIHY
                     GHTNASFIKYNANNINYSNKSTPFAPKNTLSLGIETKVPITEEWLDEIVLSAHYTGIG
                     PLYWNAQNTLSQEYYGIANGDITFSKNNTHLKFWVKNALNQSYNVFYFESLGNSFVQK
                     GIPISFGIHVKHIL"
BASE COUNT         1017 a          458 c          506 g         1068 t
ORIGIN      
        1 gctattgccg attacaatag cgcaattgat gttaatccaa gttataccaa agcgtattat
       61 aacagaggta ttgtgaagat gatgttattg gattatcaag gggcaattat tgactacaat
      121 agagcattag aattaaattc aaatgatgcc gaaacctata ataacagagg aatatgcaaa
      181 gaaagtgtaa atgattatca aggggcttta attgattata ataaagctat tgagcttaat
      241 ccatcatttt ctgaagcgta taacaatagg ggtgtgttat atcgtaaaat gaataattat
      301 aaagcagcaa tggctgatta tgacaaatta cttgttttaa ctccgaaaaa tgcagaagcg
      361 tattatcata ggggtattgt gaaatactat atttctgatt tatctggatc tattactgat
      421 tttacacaag caataacatt taactcccaa ctctttgaag catatatgta tagaggtata
      481 gtaaaaaata tgcttaatga ttttagtggg gctattttag attacaatga agcaatcaaa
      541 ttaaacccag aatctgctga agcgtattac aataggggtg tatctaaagg aagattgcaa
      601 gaacatcaat cctctattga agattttaca caggctatga attataaaat gaattatgca
      661 gaagcaattt ttaatagagg agtatcttat ttagcgttag ctcaaaaaga tgcagcatgt
      721 gcagattttt ccaaagcatc tcaacttggt ttttataaag cttttgaggt cattgccgtt
      781 ttctgcaaat aaaaatcaag agaaaaaaag aaaatacaat gggattagca agaatcctat
      841 cataatggaa tagctcgtta ttttaattgt aatagagatt aatttttgat attttttatc
      901 aatgttgatt ttttttatca tagatacaaa tccgttttgt aaatgcacac caagtaatat
      961 cccagaaaac atatatagca aggagtatag ccaatttctc gaaaagaaat gactgataaa
     1021 ctggtagata tcaggcttta ataccccatc taactctacg tattctattg tccgttttac
     1081 tttaaattgc aatacatgca ataccgtaaa tatcaataaa agaaatccca taattatcat
     1141 gtctttagaa ccttgcttgt ttcgtatttt gttaaaggct agtatatttg ccaataaaat
     1201 atgtaacacg aaacccaatg caaaagtctt ttccagggta tatgctacac tattatgttg
     1261 cataaaatag tgcattgaat tgtaataata tccctgtgtg ttagcaatag catctagtac
     1321 aagtaataag ttaacagcta aatgtaatac aataaataag agcgtgcaaa tacctgcaat
     1381 actagatatg attgaaataa ttggtttatt catttttaaa atctaaaaaa atacaaagat
     1441 gcaaatttaa tcaaaaagat gcatctttgt ataaatgggt ttacaaaatg tgtttcacat
     1501 gaatgccaaa agatatcgga ataccttttt gtacaaaact atttccaagt gattcaaaat
     1561 aaaacacatt gtacgattga ttgagtgcat ttttgaccca aaatttcaga tgtgtattat
     1621 tttttgagaa tgttatatcg ccatttgcaa taccataata ttcttgagaa agcgtatttt
     1681 gtgcattcca gtatagtgga ccaatgcctg tgtaatgtgc agaaagaacg atttcatcga
     1741 gccattcttc ggttattggt accttagttt caatacctaa agagagtgtg tttttaggtg
     1801 cgaaaggtgt tgatttgttg ctgtaattaa tgttattcgc attgtattta ataaagcttg
     1861 catttgtatg tccgtaatgg atataccccg ctatttgttt acttacatat gtctttacac
     1921 ttagttctcc acctatgctt tggcttactt ctgcattgct aatcatacga cttccattgc
     1981 tagtggttgt actcaattgt tggttatagt gatgaatata aaaaattgca gtttggtaat
     2041 taaaacgatt gttcccgaat gtaccattac taccaacttc aatattccat gattcttcgg
     2101 gcttataata tattttgtca tcaatcgtta catctttgtt accttgcatt tgtgcttgta
     2161 aaatatcaga gaacatcgaa taattatatc ctccagcttt atatcctttg gttaatgaag
     2221 tatatactct gtgttgatta tctattttat agtgtaatgc aactttcggt aatacttcta
     2281 taaagtcttt gcttttaaag cctttttcgg catatgttga gtccacccga aaagcaggaa
     2341 caaatgggaa aggtaatttt acacttgtct gcaaggttgc ttccgaatcg tagtcgataa
     2401 atgctttttc ataatccaat cgaattccag cggttacact gaatttattg gtgaatgaat
     2461 agcttgattg gtggtacaaa gctgcaccaa tagatggttt actaaataat ccagcaacaa
     2521 gcatttcttc atttaaaata gtaattgtag gcatactggg attaaacatt tttgccatat
     2581 ccataaagtt ttgtatcatc tttataccac ctttttggaa tgtcatcgga gaatcaatgg
     2641 tattacttgc tccaaataaa aataagccat ttacccattg ccaattattt gaattatttg
     2701 aacgaaatac aaattcctgc gtaatgctct ttaaatgttg attttgttgt aataccataa
     2761 tagagtcgga agtaaaatct tgatctatat ccattctatc cgtaatgttt tgaaaaccag
     2821 ttgcagaagt aaatgcataa ttatccgtta ttttttgaag tgtgaatcca gaagataaca
     2881 ttttacgtct atatgcacct tgttcgttgt agttaatagg atatatttgc ttgcttattg
     2941 tgtcaaattt ggcatacggg taggctattt ggtcggtgta atcgtaagaa atttgaaaca
     3001 ttgattgcca attgtttttc attttccatt ccagttttcc tctaatacc
//
LOCUS       DAOSMY010000187         3035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32397_length_3035_cov_3.536242, whole genome shotgun sequence.
ACCESSION   DAOSMY010000187 DAOSMY010000000
VERSION     DAOSMY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32397_length_3035_cov_3.536242"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLD76_04890"
     CDS             <1..1186
                     /locus_tag="PLD76_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27371.1"
                     /translation="DDAYIEIGKTYVVLKENDKAIETFAEIEKKFSRNHQLIRQARLQ
                     IAMLQYNDGKTDAALESYKQLVRTYPSSEEARISLEAIENILVEDNRVSEYTEFVNAL
                     GKGSIQIAPSREDSLLFRAAQRVYSRNEIPQAISSFEKYLDTYPKGRYSTTARYLLAS
                     CYYTQKQPAKALPLYKALLSDKENPNQEETLARAAEISYDLGQYAESMEYFKQLEAIG
                     SSENKMVARIGLMRASYILKRYDETVTYATNFISLHSNNLELLREAQHKRMLSYLELN
                     KAQNALPDMQILAKDTRNAFGAEAKYLLATHYYLAKQYESAEKEVFDFIDKGTPHHKW
                     LAKAFILLADVYMEQDNYFEAKQYLLSLKSNYPKDDVEIASEINTRLEEIAKIENEKV
                     NN"
     gene            1240..3000
                     /locus_tag="PLD76_04895"
     CDS             1240..3000
                     /locus_tag="PLD76_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27372.1"
                     /translation="MKRIIYILPLVLFLANTSLSFSQSNVSRDVTLEREYNPTAIDAK
                     KINTVPKTEEYTIDKKDITYTISSSPATVDKSFNPLATDTYKDTQLGEIKNGVFRAGI
                     GSHWQLLGDFYYPLLQGDTYLLDLNVSHQSAWGKLKIEDNEPTRAMFATTRAGFTFEN
                     QFRNSRLVSGIHFLHKGFDYYGKSNIDTSLWVGDTQREIVKIKADSLLESGIGSFTKA
                     GTNFRLFSTNQQSSFQHNSNLSYQYFASHIAVEEHNFYVGTNLSGSLETGRIGVSVDV
                     DNYFYKKPLELTANYAIKETDFGNTSVIKLTPYYVFVGKNWDISMGASLFAIIGATQR
                     PVSGAANIVGKAALVPDLFYLYGGIVGDIKHNSYSSILTENNYVSPDVSLQDTYTPLN
                     IYTGLKLKVMDGLLFDGYLGYKIIRDQYFFINRTVNYVAPMPIGTPASAYANTFDVVT
                     EKDASLLSVDLSLTFDKIDGLDVSVNSRYNKWNVSQNAFAWHKPTWEIGLQASYQIDA
                     KWKAGIAYNYMGGRNALVDTEVVAMNNIHDVNATVSYKVFDWLHVFGSLRNVINANYD
                     TYYGYKAFGINGMVGASVTF"
BASE COUNT         1055 a          512 c          581 g          887 t
ORIGIN      
        1 agatgacgct tacattgaaa ttggtaaaac ctacgtggta ttaaaagaaa acgataaggc
       61 tattgaaacc tttgcagaaa ttgaaaagaa attctcccgt aatcatcagc ttatacgaca
      121 agcacgctta caaattgcga tgttgcaata caatgatgga aaaacagatg cagccttaga
      181 gtcgtacaaa caacttgtac gtacctatcc ctcaagcgaa gaggctagaa tatcgttgga
      241 agctattgag aatattttgg tagaagataa tcgtgtaagt gaatataccg agtttgttaa
      301 tgctttggga aaaggctcta tccaaatagc tccttcgaga gaagactcat tattgtttag
      361 agcggctcag cgtgtctatt cgcgcaacga aataccacaa gcaatttcaa gttttgagaa
      421 atatttagat acctatccca aagggcgtta tagtactact gctcgctatt tgttagcaag
      481 ttgttattat acacaaaaac agcctgcaaa agccctccct ttatataaag cactgctatc
      541 tgataaagag aatccaaatc aggaagaaac cttagcaagg gcggcagaaa taagctatga
      601 tttggggcaa tatgcagaat caatggaata ttttaaacaa ctcgaagcga ttggttcgag
      661 cgaaaataaa atggtagcac gcatcggact gatgagagca tcgtatattt tgaaacgcta
      721 tgatgaaaca gttacgtatg ctactaattt tatatccttg catagcaaca accttgaatt
      781 gcttcgcgaa gcacaacaca aacgtatgtt gagttattta gagctaaata aagcacaaaa
      841 tgcactgcca gatatgcaaa tcttagctaa agatacacga aatgcctttg gagcagaagc
      901 taaatactta cttgctacac attactacct agccaaacaa tatgaatcag cggaaaaaga
      961 agtgtttgat tttatagata agggaactcc gcatcacaaa tggttagcca aagcctttat
     1021 attgctcgcc gacgtatata tggaacagga taattatttt gaagcaaaac aatacttatt
     1081 aagtttaaaa agtaattatc ccaaagacga tgtagaaata gcatcagaaa tcaatactcg
     1141 tttggaagag attgctaaaa ttgagaatga aaaagtgaat aattaataaa gttagtaagc
     1201 cagaatcgtg cgtaatcaca atcctataaa caaaattgta tgaaaagaat aatctatata
     1261 ctacctttgg tactattttt agccaataca tcgttgagtt tttcgcaatc aaacgtttca
     1321 cgagatgtaa ctttagaaag agaatacaac cctacagcaa tagatgccaa gaaaattaat
     1381 accgttccta aaacagaaga atatactatt gataaaaagg atattacata taccattagc
     1441 tcttctccgg caacagttga caaaagtttt aaccctttag caaccgatac gtataaagac
     1501 acacaattgg gagaaataaa aaacggagta tttagagcgg gtattggtag tcattggcaa
     1561 ttactaggag atttttatta tcctctttta caaggagata cctatttact tgaccttaat
     1621 gtatcacatc aatctgcgtg gggaaaatta aaaattgagg ataatgaacc tacgcgagct
     1681 atgtttgcta ccacacgagc aggatttaca tttgaaaatc aatttcgtaa ttctcgtctt
     1741 gtttctggta ttcatttttt gcataaaggg tttgattatt atggaaaatc aaacatagat
     1801 acctctttgt gggtgggaga tacccaacgc gaaatagtaa aaattaaagc cgacagtttg
     1861 ctcgaatcag gcattggaag ttttacaaaa gcgggtacta attttcgcct attttctaca
     1921 aaccaacaat cttcttttca gcacaatagc aacctttctt atcagtattt tgcatcacat
     1981 attgcagtag aagaacataa tttttatgtt ggtacaaacc ttagtgggtc gctagaaaca
     2041 ggaagaatag gcgtttcggt tgacgtggat aattattttt acaagaaacc acttgagcta
     2101 actgcaaact atgctattaa agagactgat tttggcaata cgagtgtcat taaattaact
     2161 ccctactacg tttttgttgg caaaaactgg gacatcagca tgggtgcaag tttatttgca
     2221 attattggag ccacccaacg tcctgtaagc ggtgctgcaa acattgtagg taaggctgct
     2281 ttagttcccg atttatttta tctgtatggt ggcattgtag gggatattaa acacaattcg
     2341 tattcatcaa ttttaacaga aaacaactac gtgtcgcccg acgttagttt gcaagacacg
     2401 tatacacccc taaatattta cacaggatta aaattaaaag tgatggatgg gcttttattt
     2461 gatgggtatt tgggatataa aattatacgc gaccagtatt ttttcatcaa cagaacggtt
     2521 aattacgtag ctcctatgcc tataggtaca cctgcaagtg catacgcaaa cacatttgat
     2581 gtggtaacag aaaaagatgc atcgctttta tcggttgatt tatcgcttac ttttgacaaa
     2641 atagatggac tagatgtatc ggttaacagt cgttataaca aatggaatgt aagtcaaaat
     2701 gcttttgcgt ggcataaacc aacatgggaa attggactac aagccagcta tcaaattgat
     2761 gctaaatgga aagcaggtat tgcctataac tatatgggag gtagaaatgc cttagttgat
     2821 acagaagtgg tagccatgaa caatatacat gatgtaaatg ctactgtttc ttataaagta
     2881 ttcgattggc tacacgtatt tggatctttg cgaaatgtaa tcaatgcaaa ttacgatact
     2941 tattacgggt ataaagcctt tggtataaat ggaatggtag gtgcttcggt tacattctaa
     3001 agtacatagt cctttttttt taagggaaag aagaa
//
LOCUS       DAOSMY010000188         3034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32414_length_3034_cov_2.987580, whole genome shotgun sequence.
ACCESSION   DAOSMY010000188 DAOSMY010000000
VERSION     DAOSMY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32414_length_3034_cov_2.987580"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /gene="prmC"
                     /locus_tag="PLD76_04900"
     CDS             <1..838
                     /gene="prmC"
                     /locus_tag="PLD76_04900"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU27373.1"
                     /translation="KLFFTQSLSSLYPPNEIESLFRWTISHVLGIPFSHTIAYKDIKI
                     TNTQEIDIKTIVKKLQQTQPIQYIFGETEFFGLSFHVNSDVLIPRPETEELVEWILAD
                     HKQEGASKRILDIGTGSGCIAISLAKNIPTSCVTALDVSHNAIEVAKRNAHNHELTIQ
                     FIEKDILHLKNGDLNEQFDVIVSNPPYVCENEKSTINNHVLLHEPHLALFVPNDNPLQ
                     FYEAIALFAHTNLAINGAIYVEINYLFGKETQQLFHQYGFSTHLKKDIAGKDRMIKAW
                     KN"
     gene            826..1302
                     /locus_tag="PLD76_04905"
     CDS             826..1302
                     /locus_tag="PLD76_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOU27374.1"
                     /translation="MEKLDRIKSPIFLKTALYCSSAERCKSEVIERLKKETNSLDEIE
                     EIVAILEEEGYINELRYAISYVRDKFRFNKWGKIKISYMLQCKKIPSSIITEALAEID
                     DDTYLETLTALVLSKSKSIKSTNPSDKKAKIYRFAASRGFESESIRQALIQCRVDK"
     gene            complement(1820..>3034)
                     /locus_tag="PLD76_04910"
     CDS             complement(1820..>3034)
                     /locus_tag="PLD76_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU27375.1"
                     /translation="YVNINTTTGGVTWAAPFTQLTEPVGPQQIFAIRVPDQYGNVRPS
                     AFKLPAVIYYRNGTDEQKALGTAPIKYTFNGRLSDEAGLRQYTIESAHQLLSNTFPAN
                     LDAAKVAAAGGTVKVYDYGLKSFVAAEGGETIKPMHGFIYSRSNAEQTTLQVTDAMIV
                     FGNTKYRKRTVTQPLFRMNVQNTNNTYGSRIGVKYKADKADEFDGTIDAEKLFNDQDL
                     TVPEVYMLAYSKKLETKGINQATEIIPLGIRTNAEKSLKFTRYSTDGYEEVILEDRQT
                     GNSYDMLATNAEYIINNVPSGNNEGRFYLHLTVAEDRIPSTIGEETIGSADAEELDIA
                     IFAQGKQVTVSSSQNLVIETILVSDMAGRTQVFTATNPHFNQLNLQVAQGTYIVKVIG
                     DKGTKEAKVIIK"
BASE COUNT          969 a          540 c          518 g         1007 t
ORIGIN      
        1 taaattgttt tttacccaaa gtctttcttc tttatatcct cccaatgaaa tagaaagcct
       61 atttcgatgg acaatctcgc atgtattggg tattcctttc tctcatacca ttgcatacaa
      121 agatataaaa attactaaca cgcaagaaat tgatattaaa acaattgtaa aaaaattaca
      181 acaaacacaa ccgatacaat atatttttgg tgaaacagag ttttttggat tatcattcca
      241 tgttaattcg gatgtgctca taccacgtcc cgaaacggaa gagttggtcg aatggatttt
      301 agccgatcat aagcaagaag gtgcatccaa aagaatactt gatataggta ctggaagtgg
      361 atgtatcgcc atttcgttag caaaaaatat cccaacttct tgtgtaacag ctctagatgt
      421 gtcgcacaat gccattgaag tagcaaaacg taatgcacac aaccatgagc ttacaatcca
      481 gtttattgag aaagatatat tacatctaaa aaacggcgat ttaaacgaac aatttgatgt
      541 tattgtgagt aatccgcctt atgtttgtga aaacgaaaaa tccactatca ataatcatgt
      601 tttgttgcac gaaccgcatt tggctttgtt tgttcctaac gataatcctt tgcagtttta
      661 cgaagcaatt gctctatttg cccatacgaa tttagcaata aatggagcta tatatgtgga
      721 aattaattac ctctttggaa aagaaacaca acaacttttc caccagtatg gctttagtac
      781 acaccttaag aaagatattg ctggcaagga tagaatgata aaggcatgga aaaactagac
      841 agaataaaat cccccatttt tttaaaaaca gctctctatt gttcttcggc tgaaagatgc
      901 aaaagcgagg taatagaacg tttaaagaaa gagaccaatt ctttagatga aatcgaagaa
      961 attgtagcta ttctggaaga agagggatat atcaatgaat tacgttatgc cataagctat
     1021 gtacgggata aattcagatt taataaatgg ggtaaaataa aaatttccta tatgttacaa
     1081 tgtaagaaaa taccctcgtc aattattaca gaagctcttg ccgaaataga cgacgatact
     1141 tatctggaga ccttaacagc cttggttctc tccaaaagta aatccattaa atcaaccaat
     1201 ccatcggata aaaaagcaaa aatatacaga tttgcagcaa gcaggggttt tgaaagcgaa
     1261 agtatccgac aagccctcat tcagtgcaga gttgacaagt gacagttgaa agtgaaaagt
     1321 tagatagttg ataattgata attgatagtt gatagttgaa agtggttagt ggttagtggt
     1381 taatggttaa tgtttagtgg ttaatggtta gtggttaatt gacagttgaa agtgtttagt
     1441 gtttaatgtt tagtgtttag tgtttagtgt ttaatgttta atgttgagcg ttgctcgagc
     1501 aaaactatca actgtccact ttcaactatc ccctgaaaaa aggagcgtag ctctgctatc
     1561 ttcgtagcaa aatacaatac gtcatattat taggggcgtt agccctgaca tcttagttga
     1621 taattgatag ttgatagttg aaagtggtta atgtttaatg tttagtgttt aatgttttgc
     1681 gttgctcgag caaaactatc aactatcaac tatccacttt caactttcaa ctttcaactt
     1741 tcaactgtcc actatcaact ttcaactaaa aaaggaagaa ctttcgttct tccttttttt
     1801 atattcagca ttattgcaat tatttaataa tcactttcgc ttctttagtt cctttatcgc
     1861 caattacttt cactatataa gtaccttgtg ccacttgtaa atttaactgg ttgaagtgag
     1921 gattagtagc tgtaaacact tgcgtgcgtc ctgccatatc acttactaaa attgtttcaa
     1981 ttacaagatt ttgcgaagaa cttactgtta cttgtttacc ttgtgcaaag atagcaatat
     2041 caagctcttc tgcatccgca gagccaatgg tttcttcacc aatagtactt gggatacgat
     2101 cttcagctac agtaagatgt aaatagaaac gaccttcgtt attacccgat ggcacattgt
     2161 taataatata ctcagcattt gtagctaaca tatcgtatga atttcctgtt tgtctatctt
     2221 ctaagatcac ttcttcataa ccatctgtac tataacgtgt gaacttcaat gatttttcag
     2281 catttgtacg gatacctaaa ggaattattt ctgttgcttg attaattcct tttgtttcaa
     2341 gttttttact ataagctaac atataaacct ctggtacagt taaatcctga tcattaaaca
     2401 atttttcagc gtcaatcgtt ccatcaaact cgtccgcttt atcagcttta tattttacac
     2461 caattcgact accatatgta ttattggtat tttgtacatt catacggaac aatggttgtg
     2521 ttactgttct ctttctgtac ttcgtattac caaatacaat catagcatct gttacttgta
     2581 aagtagtttg ttcagcatta ctacgtgagt aaataaatcc atgcattggt ttgatggttt
     2641 ctccaccctc agcagctaca aatgatttta aaccataatc atacacttta actgtaccac
     2701 cagcagcagc tactttagca gcatctaagt ttgctgggaa ggtattgctc aataattgat
     2761 gtgccgattc gatagtgtat tgacgcaatc cagcttcatc tgataagcga ccattaaatg
     2821 tatatttaat aggagctgta cccaatgctt tttgctcgtc tgtaccattt ctgtaataaa
     2881 ttacagcagg taatttaaat gcacttggcc ttacattacc atattggtca ggcacacgaa
     2941 ttgcaaatat ttgttgtggt cctacaggct cggttaattg tgtaaatgga gcagcccaag
     3001 ttacaccacc tgtcgtagta ttaatattta cata
//
LOCUS       DAOSMY010000189         3007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32777_length_3007_cov_2.980014, whole genome shotgun sequence.
ACCESSION   DAOSMY010000189 DAOSMY010000000
VERSION     DAOSMY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32777_length_3007_cov_2.980014"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..2143)
                     /locus_tag="PLD76_04915"
     CDS             complement(95..2143)
                     /locus_tag="PLD76_04915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOU27376.1"
                     /translation="MTSYANPFLTARYTTPYQTVPFNEIKEEHYKEAFLKGIEEQLTE
                     IDSLLANKTTPTFENTIVALEKTGDLISRTSAVFFNLLHAETNDTLENLAQELSPILT
                     EHSNKIYQNQALFERVKAVNAQKETLTLTAEQQQLLENTYQAFVRSGAELSEKDKQTY
                     TELSTKLSQLTLTFSQYVLKETNAYSYVITDEKELDGLPDYAKEAAAEKAKAKNQEGW
                     LIDLSMPSYLPIMQYATNRELRKKLYFAYNTKGNQGNEYDNKQTITDIVNTRLQLAQL
                     MGYSNYATYKLVRTMAQDTSKVYALIDQLYTAYQPVASQEFDEIQAFARTFENNATGE
                     LMPWDWAYYAEKVKEQKYQVNDELLKPYFELEKVKQGVFGLATALFDLQFTKNTSIQV
                     YHPDVDAYEVHDSKGNFIAILYTDFHPRESKKGGAWMTEFKEQWIENGENSRPHISIV
                     MNFTPPTATSPSLLTFNELTTLLHEFGHALHGMLSNTTYASLSGTSVYRDFVELPSQL
                     LENWATEKEFLDTFAFHYQTGEPIPSQLIEKIKEAEQFLVGYACLRQLSFCYLDMAWH
                     TLSTPFSGDVKSFETDAWKKTLLFEPIAETCMSTQFNHIFSGGYAAGYYSYKWAEVLD
                     ADAYEVFKQQGIFNKKTATSFAENILSKGGSEHPMILYKRFRGQEPTIDALLKRNGIK
                     "
     gene            complement(2209..>3007)
                     /locus_tag="PLD76_04920"
     CDS             complement(2209..>3007)
                     /locus_tag="PLD76_04920"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HOU27377.1"
                     /translation="ARAKQKLNLLFKNEQKIVKAEGETILRKALTEAGFVIKKDISND
                     IIKKLLHHFEINSFEELTFQIGKGSISLANIQDIVKQENKKTTVRSWYPRFFFGKDTD
                     KENTATASARIKKNGTYQLTDEMLQKEHTIAPCCKPIPGDQIIGFINDKKEIIVHKRD
                     CKEALKLKSQKGSSIITAEWANTQKKYYATTIEVQGVDRKRILLDILTEISDKLDANI
                     EKLCVETNDGIFSAKMNVYVSHVDTINQIYKQLKIINGVQRVARIEE"
BASE COUNT          768 a          519 c          596 g         1124 t
ORIGIN      
        1 acatctgtgt cgggtgttgt ggcaaacgtc gcactactaa tacacacaac aaacgctatt
       61 cctgttagta ttcttttcat ggtaaactca ttttttattt aattccattt cgtttgagta
      121 gggcatcgat agtcggttct tgacctctaa atcgtttata caaaatcata ggatgttcgg
      181 agcctccttt gcttaaaata ttttctgcaa atgatgtagc tgtttttttg ttaaaaattc
      241 cttgttgttt aaatacctca tatgcatcag catccaatac ttctgcccat ttgtagctgt
      301 aatatcctgc tgcgtatcct cccgaaaata tatggttaaa ttgtgtgctc atacacgttt
      361 cggcaatggg ttcaaagagc aatgtttttt tccaagcatc tgtttcaaag ctttttacat
      421 cgccactaaa aggtgttgaa agcgtatgcc atgccatgtc taagtagcaa aaacttagtt
      481 gacgcaaaca agcataccct accaaaaatt gttccgcttc ttttatcttt tcaatcaatt
      541 gagatggaat tggttcgcct gtttgataat gaaaagcgaa ggtatcaaga aattcttttt
      601 ctgtagccca attttcgagc aattgagagg ggagttctac aaagtcgcgg taaacgcttg
      661 ttccagagag gcttgcgtag gtggtgttgg ataacatacc gtgcaaagcg tgtccaaatt
      721 cgtgtagcaa ggtcgttaat tcattaaaag tgagcaacga cgggctagta gctgtgggtg
      781 gtgtaaagtt cattacaatg gatatgtggg ggcgactatt ttctccgttt tcaatccatt
      841 gttctttaaa ctcagtcatc catgcacctc cttttttgct ttctcttgga tgaaaatcgg
      901 tgtataatat ggcaataaaa ttccccttgc tatcatgcac ttcataggca tctacatctg
      961 gatgatacac ttgtatagaa gtgttttttg taaactgtaa atcaaaaagg gcagtagcta
     1021 gaccaaacac tccttgtttt actttttcga gttcaaaata gggtttgagt aattcgtcgt
     1081 ttacttggta tttttgttct ttcacttttt cggcataata tgcccaatcc caaggcatga
     1141 gttcacccgt agcattgttt tcaaacgtgc gagcaaaggc ttgtatttca tcaaattctt
     1201 gcgaggcaac tggctgataa gcagtatata gttggtcgat aagtgcatat accttgctgg
     1261 tgtcttgagc catggtacgt actagcttgt aggtagcgta gttgctgtaa cccattagtt
     1321 gagcaagttg cagacgggtg ttaacaatat ctgttatggt ctgtttgttg tcgtattcat
     1381 ttccttgatt ccctttggta ttgtaagcaa aatatagttt tttgcgtaac tcgcggttgg
     1441 ttgcatattg cattatgggc aaataacttg gcatcgaaag gtcgataagc caaccttctt
     1501 gatttttggc tttggctttt tctgcagctg cttctttggc ataatcaggc aatccgtcca
     1561 gttctttttc gtcggtgatt acgtatgaat aagcgtttgt ttctttcagt acatattggc
     1621 taaacgtaag tgttagttgg cttagcttgg tgcttaattc agtgtacgtt tgtttgtctt
     1681 tttctgataa ttcagcccct gagcgtacaa atgcttggta ggtattttcg agtaattgtt
     1741 gttgttcggc tgtcagagtt aaggtctctt tttgagcatt tacggctttt actcgttcaa
     1801 aaagggcttg attttggtaa atcttattgc tgtgttcagt gagtatcgga gatagttctt
     1861 gagccagatt ttctaaggta tcgtttgttt cagcatgcaa taagttgaaa aatacagcac
     1921 ttgtgcggct tattaaatct cctgtttttt ctaatgctac tatggtattt tcaaatgttg
     1981 gagtcgtttt attggctaag agagagtcta tttcagttag ttgctcttct attcctttca
     2041 gaaaagcttc tttgtaatgt tcttctttta tctcgttaaa aggtacagtt tggtaaggag
     2101 ttgtataacg agcggttaaa aaagggtttg cgtaactagt catagaaaaa agtattagat
     2161 aaaataaaat gtgagatata taggcattca tatgtctgta taaaggtgtt attcttcgat
     2221 acgtgctaca cgttgtacac cattaataat tttcagttgc ttgtatattt ggttaattgt
     2281 atctacgtgg cttacataca cgttcatttt ggcagaaaaa ataccatcgt ttgtttctac
     2341 gcataatttt tcaatattag catctagttt atcagatatt tcagttaaaa tatctaacaa
     2401 aatacgtttt ctatctactc cttgtacttc gatagtggta gcataatatt ttttttgtgt
     2461 attagcccat tcggcagtaa tgatgctcga ccctttttgc gatttgagtt ttaacgcttc
     2521 tttgcagtct cgtttgtgta cgattatttc ttttttgtca tttataaaac caataatttg
     2581 atcgcctggt atgggtttgc aacacggagc aatagtgtgt tctttttgca gcatttcatc
     2641 tgtaagctgg taggtgccat tctttttaat acgagccgaa gctgttgctg tattctcttt
     2701 atccgtatct ttgccaaaga agaagcgagg ataccacgaa cgaacggtgg tttttttatt
     2761 ctcttgtttt acaatatctt gaatattggc aagagaaata ctgcctttgc ctatttgaaa
     2821 agtaagttct tcaaaactgt tgatttcaaa atggtgcaac aattttttga ttatatcgtt
     2881 gctaatatct ttttttatta caaatccagc ttctgtaagt gctttgcgta ggatagtttc
     2941 cccttctgct tttactattt tttgttcgtt tttaaaaagc aaattaagtt tctgttttgc
     3001 tcgagct
//
LOCUS       DAOSMY010000190         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32785_length_3006_cov_3.540834, whole genome shotgun sequence.
ACCESSION   DAOSMY010000190 DAOSMY010000000
VERSION     DAOSMY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32785_length_3006_cov_3.540834"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLD76_04925"
     CDS             complement(<1..913)
                     /locus_tag="PLD76_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU27378.1"
                     /translation="MKRKLFLLFASFFLGYSVHSIAQESLHISIDEAKEYALNYNKTI
                     QKANLAIQKADYAKWQSIASLLPKVDASLGYNNYLGHKMTLMGMPIAMNPEGSFTAQA
                     SIALSGMQVVGVQLSEIAQQMSQTSALSDALSLQTNVTNAYYAILVSEESKKIVEGNL
                     ENLRKLYNSSLNLVKVGMAEQTDADQLAVQVGMIENTIRMAERNVELSYNTLRLLLGV
                     DNNTPLSLKNTLKDFTNKESANELLATSFDMTQNYTVTLLQQTIEMNQKQVRLNQWAY
                     APMLTGFYQYTKKTHFDKAVGFDMTPPN"
     gene            complement(933..1541)
                     /locus_tag="PLD76_04930"
     CDS             complement(933..1541)
                     /locus_tag="PLD76_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU27379.1"
                     /translation="MDKIKQEILKTATNAFVKKGIRSVSVDDICDNLRISKKTFYSYY
                     RQKEALVMAVMETFTTHHQEYHCMLQNQEFANVIDEILWMKTLIKNGKEEKKHERFFN
                     DLLNYYPSIYNTFSVEKRQYTEQMFLIQIEKGIVQGVFRGDIHVQFTAQSLTVSLQYF
                     INMSIKHPRKYTKRGIIDFMVDSYLRMVCNEKGLLYYTSLLT"
     gene            complement(1836..>3006)
                     /locus_tag="PLD76_04935"
     CDS             complement(1836..>3006)
                     /locus_tag="PLD76_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOU27380.1"
                     /translation="IENTFNSAIKVVSIAMNAKEGILQTGHLADVVMWIDDTTGKWSS
                     STYYNEQLPSWVNRKNEQNAIQNYMEMVWEPLYPINYYYVTQSRGFTTKSFSYPLKKM
                     TMGKNGVKEFKKTPFANTCIREMAVEAIEQETLGRDIFPDLLLLNFRLQSYPQASDLT
                     VELEDAYVRLDNDIKMILEAVERKIGKEHILVYITSPRKTHYAVSPLLKEKMQLGTFN
                     SYRYMALLNVYLMAIYGQESWVMGFYNGNIYLNRKIIDEKNLSLKEVQERAMEFVSQL
                     PGVMNVVNSVALEQQFALRNSLRQSYNKQSSGDILFTLYPGWVEVDKNDNPTGFYNVL
                     GNSVPFYFYGWKIQPQTIATPYAIIDATATLAKWLQIPLPNASNGVLLPIVLSAE"
BASE COUNT          946 a          555 c          460 g         1045 t
ORIGIN      
        1 aattaggtgg agtcatatca aagccaaccg ctttatcaaa atgtgttttc ttcgtatatt
       61 gataaaaacc agtgagcatc ggtgcgtacg cccattggtt taatcgaact tgcttttgat
      121 tcatttcgat agtttgttgc aacagtgtaa ccgtataatt ttgtgtcata tcaaacgatg
      181 tagccaatag ttcattggca gattctttgt ttgtaaaatc ttttagcgta tttttcaatg
      241 aaagtggcgt gttattatca acacccaata acagcctcaa ggtattgtat gataattcta
      301 cattcctttc tgccatacgt attgtatttt caatcatacc tacttgcact gctaattggt
      361 cagcatctgt ttgttctgcc atgcccactt ttacaagatt aagcgaagaa ttgtatagtt
      421 tgcgtaaatt ttctaaatta ccttcaacta ttttttttga ttcttcagac actaatatgg
      481 cataatacgc attggttaca tttgtttgca agcttaatgc atccgacaac gcagaagttt
      541 gcgacatttg ttgagctatc tcgctcagct gcactcctac tacttgcata ccactgagtg
      601 caatggaagc ctgagcggta aaactcccct cgggattcat agcaataggc atacccatta
      661 atgtcatttt atgacccaga taattattat agcccaatga tgcatccact tttggtagca
      721 aactagcaat agactgccat ttggcataat ccgctttttg aatggctaaa ttggcttttt
      781 gaattgtttt attgtagttt agagcgtact cttttgcctc atcaatgctt atatgcaaag
      841 attcttgagc tatactatgc accgaatagc caagaaagaa tgaggcgaac aatagaaata
      901 attttctttt catctgatat gtttttattt ttttatgtta ataatgatgt ataatacagt
      961 aatccttttt cgttgcacac catacgcaaa taactatcaa ccataaaatc aatgatgcca
     1021 cgtttggtgt atttgcgagg atgcttaatg gacatattaa taaaatattg caaggaaaca
     1081 gttaacgact gagccgtgaa ttgaacatga atatctcctc taaaaacacc ttgtactatt
     1141 cctttttcta tctgaataag aaacatttgt tctgtatatt gtcgtttttc tacagaaaag
     1201 gtattgtata ttgaaggata ataatttagc aaatcattaa aaaatctttc atgttttttt
     1261 tcctctttgc cattttttat caatgttttc atccacaaaa tctcatcaat cacattggca
     1321 aactcttggt tttggagcat acaatgatac tcttggtgat gtgtggtaaa cgtttccata
     1381 acagccatta ctaatgcttc tttttgcctg taataactat aaaaggtttt tttagaaata
     1441 cgtaaattat cacaaatatc atctacgctc acacttctaa tacctttttt aacaaaagca
     1501 ttggtagcag tctttaagat ttcttgttta attttgtcca tatactaaaa atacatttgt
     1561 ctctttcact ctgaaatagg acgcaaagat agaatacgaa caataaatta ccacaaacat
     1621 taatgttaaa ttattgtaat aaaccattaa acactgatta tcaaacaatt aatggaaacg
     1681 aaatattggt tacagtttcc aagtttccat ttcgaagaac aaaaattgga taaaaaacaa
     1741 cacttttaga gtgaaacctc attaagaatt gaaatttaaa tcattagtat gataaattat
     1801 accaaatttg acaataaact taggaaagaa aaattctatt ctgcagataa aacaattggc
     1861 aacaacacac cattactagc gttgggcaaa ggaatctgta accatttggc aagtgttgct
     1921 gtagcatcta ttatggcata cggtgtagcg atggtttgtg gctgtatctt ccatccataa
     1981 aaataaaaag gaacactatt gcctaataca ttatagaaac cagttgggtt atcgttttta
     2041 tctacctcaa cccaacctgg atataaggta aataatatat ctcctgagct ttgcttgttg
     2101 taggattgtc gaagcgaatt tcttaacgca aattgttgct ccaaagcgac tgaattaacc
     2161 acattcatca caccaggcaa ttgtgataca aactccatcg ctcgttcttg cacttctttt
     2221 aaactcaaat tcttttcgtc tattattttt ctatttaaat aaatattccc gttgtaaaaa
     2281 cccattaccc aactttcctg accgtatatc gccattagat aaacattcaa caaagccata
     2341 tagcgatacg agttaaatgt gccgagttgc attttttctt ttaataaagg agacacagca
     2401 taatgagttt tgcgaggaga ggtgatatat acaaggatat gttctttgcc aatttttctt
     2461 tctactgctt caagaatcat tttgatatca ttatctaatc gtacatatgc gtcttctaat
     2521 tcgacagtta aatcgcttgc ttgaggatag gattgtaaac gaaaattaag caacaataaa
     2581 tcagggaaaa tatccctccc caaagtttct tgctctatag cttctacagc catttcgcga
     2641 atacaggtat tggcaaaagg agttttctta aattctttaa ccccattttt tcccattgtc
     2701 atttttttca acggataaga aaaacttttt gtggtaaacc cccttgattg tgttacataa
     2761 taataattaa ttggatacaa gggttcccac accatttcca tatagttttg tatggcattt
     2821 tgttcgttct tgcgatttac ccaagatggc aactgttcat tgtaatacgt agagcttgac
     2881 catttaccag tggtatcatc aatccacatt actacatctg ccaaatgtcc agtttgtaat
     2941 ataccctctt tagcattcat tgctatagac actaccttga tcgctgaatt gaatgtattt
     3001 tcaatt
//
LOCUS       DAOSMY010000191         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32789_length_3006_cov_2.691969, whole genome shotgun sequence.
ACCESSION   DAOSMY010000191 DAOSMY010000000
VERSION     DAOSMY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32789_length_3006_cov_2.691969"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1645
                     /locus_tag="PLD76_04940"
     CDS             5..1645
                     /locus_tag="PLD76_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU27381.1"
                     /translation="MILAEGLTVEFGGTSLFDNISFVVNQKDRIALMGKNGAGKSTLL
                     KILAGVREPSRGKVTTPKDYEIAYLPQHLMTEDSRTVFEEASQAFSSVFEMEKKINDL
                     NEQLAIRTDYESDSYMALIEEVSALSEKFYSIEEINYDGEIEKILLGLGFSRTDFNRK
                     TSEFSGGWRMRIELAKLLLRNPQAILLDEPTNHLDIESIQWLENFLIQSDKAVIVISH
                     DRAFVDTITNRTIEVTMGQIYDYKATYSHYLELRKERRQQQQKQYEDQQKMIAETTEF
                     IERFKGTYSKTLQVQSRVKMLEKLQIVEVDEEDTSALRLKFPPSPRSGNYPVIAENVS
                     KQYDDKLIFKDVNVMIHRGEKIAFVGKNGEGKSTLVKCIMGEIPFGGTLTLGHSVKIG
                     YFAQNQASMLDENLTVFQTIDDIAVGDIRTKIRDILGAFMFGREDIDKKVKVLSGGER
                     TRLAMIKLLLEPVNLLILDEPTNHLDLKTKDILKQALIDFDGTLITVSHDRDFLDGLA
                     TKVYEFGNQKVIEHHEGIYEFLEKKKLTNLKELERNKK"
     gene            complement(1739..>3006)
                     /locus_tag="PLD76_04945"
     CDS             complement(1739..>3006)
                     /locus_tag="PLD76_04945"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HOU27382.1"
                     /translation="ELPLLVEVDVKKLESAINQKINGLVFEGDKLNNQDLSIKIWKAQ
                     NFSFNVKNNVIEYRVPLKLWTRFAWSVDKFGLSMGDTYEATGSIALNYKTTIEIDKNW
                     KLLAKTTSHGFQWIDTPKLNVIGVSVPITPIATIALQQSEELITSQIDAILAQTINIK
                     KYVAMAWKEAQKPMQVSPENDLWVRISPKNVYVSPFTTNGQKLYVAISIQAVIESFMG
                     VQPVQNKLTVLPPFTVSANQPKDFNLNIATDITYEKISELAKSQLQNKTFSEGNKSIT
                     ITDLSVFGSEGKAIFVADVIGSIKGRIYFTGTLGYNAETMSIEVLQPEFDIKTKNALV
                     KSANWLLNGFIIKKITPYLTYPVQDELTTMKTEANKMLTNYKVYDGVDLTGKLHTISV
                     QRIDLVPGAIRLHANVGGNIALKVNDIHF"
BASE COUNT          945 a          568 c          544 g          949 t
ORIGIN      
        1 taaaatgatt ttagccgaag gactaaccgt agaatttggg ggcacttccc tatttgacaa
       61 tatttcgttt gtagtaaacc aaaaagaccg cattgcccta atgggtaaaa atggtgctgg
      121 taaatctacc ttactcaaaa ttttagcagg tgttcgcgaa ccatcacgtg ggaaagtaac
      181 cactcctaaa gattacgaaa tagcctattt acctcaacac ttaatgacag aagactctcg
      241 aacagtattt gaagaagcgt ctcaagcctt ttcttctgta tttgagatgg agaaaaaaat
      301 taatgacctc aacgaacaac ttgccatacg taccgattac gaaagcgata gttatatggc
      361 gttaattgaa gaagtttcgg ctttgagtga aaaattttat agcatcgaag aaatcaacta
      421 tgatggtgaa attgaaaaga ttttgcttgg attagggttt tctcgtactg attttaacag
      481 aaaaaccagt gaatttagcg gtggctggcg tatgcgtatc gaactagcga agttattatt
      541 acgcaatcca caagctatct tgctcgacga acctaccaac cacctcgaca ttgaatccat
      601 tcaatggttg gaaaactttt tgatacaaag tgataaagcg gttattgtca tttcgcatga
      661 tagggctttt gttgatacga ttaccaatcg tacaattgag gtaacgatgg gacaaatata
      721 tgattacaaa gctacctact ctcactacct tgaactacgt aaagaacgcc gtcagcaaca
      781 gcaaaaacag tatgaggacc aacaaaaaat gattgctgaa accaccgagt ttatcgaacg
      841 gtttaaaggc acctattcca aaaccctgca agtgcaatcg cgtgtaaaaa tgctcgaaaa
      901 attgcaaatt gtagaagttg atgaagaaga tacatctgct ttgcgactca aatttcctcc
      961 ctcgccacgt tcgggtaatt accccgtaat agccgaaaat gtaagcaaac agtatgatga
     1021 taagctgata tttaaggatg taaatgtaat gatacaccga ggcgaaaaaa ttgcttttgt
     1081 aggcaaaaat ggcgaaggta aatcaacctt ggtaaaatgt atcatgggcg aaattccttt
     1141 tgggggtaca ctcaccctcg gacacagtgt aaaaattggg tattttgctc aaaatcaagc
     1201 ctcaatgtta gacgaaaatc ttaccgtttt tcaaaccatt gatgatattg ctgtgggcga
     1261 tatacgtaca aagattcgcg atatactggg agctttcatg tttggtagag aggatattga
     1321 caaaaaagta aaagtacttt cgggtggcga acggacacga ttagccatga taaaactgtt
     1381 gctcgaacct gtaaacttgc tgatattaga tgagcctacc aaccatcttg acttgaagac
     1441 aaaagatata ttgaaacaag ccctcatcga ctttgatggc acgttaataa cggtttcgca
     1501 cgaccgcgat tttttagatg gtctggctac gaaagtatat gaatttggca atcaaaaagt
     1561 tatagaacac cacgaaggta tttacgaatt tcttgaaaag aaaaaactaa ctaacttaaa
     1621 agagttggag cgtaataaaa aatagctact agtagccaag ttctattgct atggcataac
     1681 tcaactttat atatttacag ctctttgttt ggactgccta tatgtttaca ctcccgattt
     1741 agaagtgtat atcgtttact tttagggcaa tatttccacc tacattcgca tgcaaacgta
     1801 tagctccagg cactaaatcg attctttgca cggatattgt gtgtaacttg ccagtaaggt
     1861 ctacaccatc atacaccttg tagtttgtga gcatcttgtt cgcctctgtc ttcattgttg
     1921 taagttcgtc ctgcacagga tatgtcaaat aaggcgttat tttttttata atgaatccat
     1981 ttaacaacca atttgctgat tttacaagag catttttagt tttaatatca aactcaggtt
     2041 gtaatacttc aattgacatt gtttcggcat tgtatcctaa cgttcccgta aagtaaatac
     2101 gaccttttat cgacccaata acatcagcaa caaaaatagc ttttccttcg ctaccaaata
     2161 ccgaaaggtc agttattgta atgcttttgt ttccttccga aaatgtttta ttttgcaact
     2221 gcgatttagc taattctgaa attttttcgt acgttatatc agtagcaata ttcaggttaa
     2281 aatcctttgg ctgatttgct gatacggtaa agggcggtag cactgtgagc ttattttgaa
     2341 caggttgtac ccccataaat gactctatta ctgcttgtat agaaatagca acatataatt
     2401 tctgtccatt ggttgtgaat ggtgaaacat acacattttt aggactaata cgcacccata
     2461 ggtcattttc gggacttact tgcatgggct tttgtgcttc tttccatgcc atagcaacat
     2521 atttttttat atttatcgtt tgtgccaata tagcatcaat ctgacttgtt atcaactctt
     2581 cgctttgctg aagtgcaata gtagcaatgg gtgttatagg tacacttact cctataacat
     2641 tcagttttgg tgtatcaatc cactgaaaac catgcgaggt cgttttggct agtaatttcc
     2701 aatttttatc aatctcaata gttgttttat agttcagtgc aatactccca gtagcctcat
     2761 atgtatcgcc catcgaaagt ccaaacttat ctactgacca agcaaaacgt gtccaaagtt
     2821 ttagtggcac acggtattca atcacattat ttttcacatt aaacgaaaaa ttttgtgctt
     2881 tccaaatttt tatactcaaa tcctgattat tcagtttatc tccttcaaaa actaatccat
     2941 ttattttttg attaatagct gattctaatt tttttacatc tacctctact aataatggta
     3001 actcag
//
LOCUS       DAOSMY010000192         3005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32799_length_3005_cov_2.740678, whole genome shotgun sequence.
ACCESSION   DAOSMY010000192 DAOSMY010000000
VERSION     DAOSMY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32799_length_3005_cov_2.740678"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1514
                     /locus_tag="PLD76_04950"
     CDS             123..1514
                     /locus_tag="PLD76_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOU27383.1"
                     /translation="MKNKYIDLIDQTSEFPTEEFQVVDNELSFNDIPLMDIIKQYGTP
                     LRITYLPRITQQIQKARRMFNVAMAKVDYNADYHYCYCTKSSHFSFVLEEVLKNGVHV
                     ETSSAFDMNILENLAETGHLKKDTFIICNGFKRPLYVENIQSFINQGFDNTIAILDNK
                     FELDLLLDGMEKNFKVGIRIASEEEPKFDFYTSRLGIRYNDILPYYYEKIKDNPMVDL
                     KMLHFFINTGIKDTAYYWSELNKAVELYCELKKICPELDSLDIGGGFPISTHLDFNYD
                     YEYMAEEIVAQIKNICTQNNVPEPDIFTEFGTFTVGESGAILYSIVNQKQQNDRESWN
                     MIDSSFITTLPDTWAISQRYILLAINNWDKEYQRVHLGGLTCDSEDFYSAGHSNAIFL
                     PKVDPTKGEQYIGLFHTGAYQESLAGFGGIQHCLIPAPKHVVIDLDENGEYYTKLFAK
                     EQSYKSMLKILGY"
     gene            1519..2361
                     /gene="speB"
                     /locus_tag="PLD76_04955"
     CDS             1519..2361
                     /gene="speB"
                     /locus_tag="PLD76_04955"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOU27384.1"
                     /translation="MERFAGVPDQYAVYETAKIVVLPVPYDETSSWIKGSDKGPEALL
                     DASVALEWYDIETQTQVYKKGISTALPITEKSSPELLCNAVQERVTTYLKDGKFPVTI
                     GGNHTVSIGAIRAAASQYADLTILQMDAHSDLRPSYEGSQLNHACVMYQASQLCPITQ
                     VGIRSMCEEELGLFKEDRIFYAHAIAGQTDWQQKALQTLSKNVYLTIVLDVFDPSVLP
                     STGTPEPGGLRYYEVLHFLRSVFAQYNVVGFDVVELCPNPNERASDFLAARLMYQLMT
                     YKFL"
     gene            2603..>3005
                     /locus_tag="PLD76_04960"
     CDS             2603..>3005
                     /locus_tag="PLD76_04960"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOU27385.1"
                     /translation="MKTTEKQTNKMSSNGFGFALLLIVIGAVLLLSKLQIIPAAYNDV
                     LFKWPMLLLILGIIAFVKKHITTGLVLWIVGGFFIIPIIGAVPNSFIGAVPANFTSVY
                     WPVLLILGGVLFVFHRLFGTSCCSHKHKANFH"
BASE COUNT          988 a          444 c          585 g          988 t
ORIGIN      
        1 atttgccgtt ctttcttgag caattataaa aatgtaagtg agttacattg gtaaaaaata
       61 atgaatgtaa ataagttaga ttgttttgtt gattgagtgt aaatataatt aatacttgga
      121 aaatgaaaaa taagtatatt gatttgatag atcaaacttc tgaatttcca acagaagaat
      181 ttcaagttgt tgataatgaa ctgtctttca atgatattcc attgatggat attatcaaac
      241 agtatggaac accgctgagg ataacctatt tgccacgtat tacacagcaa attcaaaaag
      301 ctaggcgtat gtttaatgtg gcaatggcaa aagtagatta caatgccgat taccattatt
      361 gttattgtac aaaaagttcg catttttcat ttgtattaga agaggtgttg aagaatgggg
      421 tacatgtaga aacatcttct gctttcgata tgaatatatt ggaaaattta gcagaaactg
      481 gtcatctcaa aaaagatacc tttattatat gtaatggatt taagcgtcca ttgtatgtgg
      541 aaaatataca aagctttatc aatcagggtt ttgataacac tattgccata ttagataata
      601 agtttgaatt agatttatta ttggatggga tggaaaaaaa cttcaaagta ggtattcgta
      661 ttgcatcgga agaggaacca aagtttgatt tttatacttc tcgtttggga attcgctata
      721 atgatatttt accatattat tacgaaaaaa ttaaagataa tccgatggtt gatttaaaga
      781 tgttgcattt ttttatcaac acaggaataa aggatactgc ttactattgg agtgaattga
      841 acaaagccgt agagttgtat tgcgaattaa aaaagatatg cccagagctc gacagtttgg
      901 atataggtgg tggatttcca ataagtactc atcttgattt taattacgat tatgagtata
      961 tggcagagga aatagtagct caaatcaaga atatttgtac acagaataat gtgcctgaac
     1021 cagatatatt taccgaattt ggaacgttta cagtaggtga aagtggtgca atcttatatt
     1081 ctattgtcaa tcaaaagcaa caaaatgata gagaatcatg gaatatgatt gatagttcgt
     1141 tcattactac attgcctgac acatgggcaa ttagtcagcg gtatatattg ttagctatca
     1201 acaattggga taaagaatac caacgtgtac acttgggagg tttgacgtgc gatagtgaag
     1261 atttttatag tgcaggacat tcaaatgcta tattcttgcc caaagttgac cctaccaaag
     1321 gtgaacaata cataggattg tttcatacgg gagcttatca ggagtcgtta gctgggtttg
     1381 gtggcataca acattgtttg attcctgctc ccaaacatgt ggtaattgac ttagatgaaa
     1441 acggtgaata ctatacaaaa ctttttgcaa aagaacagag ttataaatcg atgttgaaaa
     1501 ttttaggata ctaagaaaat ggaaagattt gctggagtac ccgatcaata tgctgtgtat
     1561 gaaacagcaa aaatagtagt tttaccagta ccgtatgacg aaaccagttc gtggataaaa
     1621 ggctctgaca aaggacctga agccttgtta gatgcgtcgg ttgcattaga atggtatgat
     1681 attgaaacac agactcaagt gtataaaaag ggaatttcta ccgccttgcc aattactgaa
     1741 aaatcatcac cagagttgct ttgcaacgca gtacaagaac gtgtaaccac ctatttaaaa
     1801 gatggaaaat tccctgttac tattggaggt aatcataccg taagtattgg agctattcga
     1861 gcggcagcat cgcaatatgc tgacttaact attttacaaa tggatgctca ttctgatttg
     1921 cgtccaagct acgagggttc tcaacttaac catgcctgtg tgatgtacca agcatcgcaa
     1981 ttgtgtccga ttacgcaagt aggtattcgc agtatgtgcg aagaagagtt aggactattt
     2041 aaagaggata gaatttttta tgctcacgcc atagctggac aaacagattg gcaacaaaaa
     2101 gcccttcaaa cccttagtaa aaatgtgtat cttacgatag ttttagatgt ttttgatccg
     2161 tcagtattgc cttcaacagg aactcccgaa ccaggaggac ttcgctacta tgaagtattg
     2221 cattttttgc gaagtgtgtt tgctcaatac aatgtagtag gttttgatgt ggtagaatta
     2281 tgcccaaatc cgaatgaaag agcttctgat tttttggctg cacgtttaat gtatcaattg
     2341 atgacgtata agtttttata agcgaaataa agtaattgtt acaaaaggga ttgttttgat
     2401 agaaaacaat ccctttaatt tttgaatttt cgccataaaa aacattaatt tcttttatat
     2461 tttactcctt tgtcgaaaaa atcggtattt ttatcgggaa ttttgtgctg ttaatcggtt
     2521 ttgtgttttt aacacttgta gatacatcta cttttgtatt gtacaaaata cacaacacat
     2581 tattcattaa aaacaaggta acatgaaaac aacagaaaaa caaacaaaca agatgtcaag
     2641 caatggtttt ggctttgctc tattattaat tgtaatagga gctgttttac tattatctaa
     2701 actgcaaata attcctgcgg catataacga tgtgctcttt aaatggccca tgttattgct
     2761 tattttaggt attattgctt ttgtaaaaaa acatattaca actggattgg tactatggat
     2821 tgtgggtggt ttttttatta tccccatcat aggagctgta cccaatagtt ttataggagc
     2881 tgtgccagca aactttacct cggtgtattg gcctgttctc ttaattcttg gtggtgtatt
     2941 attcgtgttt catcgtttgt ttggcactag ttgttgttct cacaaacaca aagccaattt
     3001 tcaca
//
LOCUS       DAOSMY010000193         3002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32834_length_3002_cov_3.840855, whole genome shotgun sequence.
ACCESSION   DAOSMY010000193 DAOSMY010000000
VERSION     DAOSMY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32834_length_3002_cov_3.840855"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLD76_04965"
     CDS             <1..431
                     /locus_tag="PLD76_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOU27386.1"
                     /translation="LKLEINCKEHFNVLDWVDFPFEIKSEWFTGKAEIRTYNINELLG
                     TKLRALYQRSKGRDLFDLDYARQNMDLDYEQIITCFKEYTSYATGKKPPSKKEFLLNI
                     EEKELSAEFIGDMEALLRPEIKYNQRDAFNWLKTELIETI"
     gene            625..1041
                     /locus_tag="PLD76_04970"
     CDS             625..1041
                     /locus_tag="PLD76_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27387.1"
                     /translation="MNKAQAQTQVIRFKRYSRKAYAAFCSMHKHITIGKLATALCDKQ
                     LAKSKLAHTNSADCLAFYTPVFSDESIVDNEMFAFDVLPLGCVAVIPTTYSTTEGQEA
                     RISCSDCLYTLLLYLMVFSTAFLFAHTLYSLNPFMI"
     gene            1038..2093
                     /locus_tag="PLD76_04975"
     CDS             1038..2093
                     /locus_tag="PLD76_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOU27388.1"
                     /translation="MNKKKILSLALLATLLPQLGMMAQSNSSNTQQLTEISVHAQPTK
                     VYSDHLRVVMSIDKSTIAQMPVQTIQELLNNLPGLDVRQRGGSGVQADISMRGGTFDQ
                     VLILLNGVAISDPQTGHHTLNLPIDLNMVERVEILQGGAISLFGTNALSGAINIVTNE
                     KADDALKVSLTGGSFGLFNPSVSSTFASNKWRWAISASHNESAGYTENTDYRYTQGFV
                     QAQFQATEQDIWKWQIGVQDKAFGANSFYSVNYPNQFEKTKTLISSISWDKRINSLLW
                     QSTAYYRGHFDQFELFRNYENAATWYTFHNYHLSHVSGINSKLSWFTSLGKSSIGIEG
                     RNEHINSNVLGELKATP"
     gene            2097..2960
                     /locus_tag="PLD76_04980"
     CDS             2097..2960
                     /locus_tag="PLD76_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU27389.1"
                     /translation="MPFAKNTNTQFLYEKNRTNVQYFAEQTIYLQAFTASVGVSGNYN
                     TQFGNRYSYGLNTSYAFNTYAQVFANINKSLRLPTFTDLYYKSATQESNPSLKPEEAI
                     TAEIGAKYTKQAWQWNANAFYRIANNSIDWVRSLETEKWKSMNHTRIDAMGGEIAAAY
                     TGGFWAKNIQASYAYVLLNKDAGELISKYALDYLQHKINLQYVHGISKQVDASWVVSY
                     QDRKGNYVNKNGNKVAYTPFATIDTRINWQQNNTKMYAEISNILNQHYYDYGGVPIAG
                     RMLKVGIAVQL"
BASE COUNT          994 a          594 c          596 g          818 t
ORIGIN      
        1 gattgaaatt ggaaataaac tgtaaagagc atttcaatgt attggattgg gtcgattttc
       61 cttttgaaat taaaagtgaa tggtttacag gtaaagcaga aataagaacg tacaatataa
      121 atgaactttt agggacaaaa ctcagggctt tatatcaacg aagcaaagga agagatttat
      181 ttgatttaga ttatgcacga caaaatatgg atttagacta tgagcaaatc ataacgtgtt
      241 ttaaggaata cacttcgtat gccacaggta aaaaacctcc tagtaaaaaa gaattcttac
      301 ttaacataga agaaaaagag cttagtgctg agtttatagg tgatatggaa gcattattaa
      361 gacccgaaat taaatacaat cagcgtgatg cttttaattg gttgaaaacg gaattaattg
      421 aaacaattta aatgagaaaa taatgttgct ctattccttt aaatacaccc agtcaggacg
      481 gagttctcaa ttaaaagtgt tcggaagact tttctgagaa ccaacgactt ggggcagtgc
      541 aagaacacat aaagtaaaaa attctgatga caaacctcgt catttaattt tttgcattac
      601 ctttgtagcg aaaatttaca ttccatgaac aaagcacaag cacaaacaca agttattcgt
      661 tttaagcgat actcgcgtaa ggcgtatgct gctttttgtt cgatgcacaa gcacattacc
      721 attggtaaac ttgccacagc actttgcgac aaacaattgg ctaaaagcaa actagcacat
      781 acaaattcgg ctgattgttt ggctttttat acaccagtat tttctgatga atcaattgtg
      841 gacaatgaga tgtttgcttt tgatgtgttg ccgttggggt gtgttgctgt tatccctacc
      901 acttattcta ctactgaggg tcaggaagct cggatttcct gctcggattg tttatacacg
      961 ctgttgttgt acctgatggt attttcaaca gcgtttttgt ttgcacatac tttgtattca
     1021 cttaaccctt ttatgatatg aacaagaaaa aaattctttc cttggcattg cttgccaccc
     1081 tattgcctca acttggaatg atggcacaaa gcaatagttc taatacgcaa cagctaactg
     1141 aaatcagtgt tcacgctcag cccaccaagg tgtattcaga ccatctgcga gtagtgatga
     1201 gtatagacaa gagcacaata gctcaaatgc ctgtacaaac cattcaagag ctacttaaca
     1261 acctgcccgg actggacgtt cgccagcgtg gtggtagtgg tgtgcaagcc gatatcagta
     1321 tgcgaggagg cactttcgac caagtgttaa tcttactcaa tggagtagct atcagcgacc
     1381 cacaaacggg tcatcatacg cttaacctgc ctatcgattt aaatatggta gagcgagtgg
     1441 aaatattgca aggcggtgcc atcagtctct ttggaaccaa tgccctaagt ggtgctatca
     1501 atattgttac caacgaaaaa gctgatgatg ccctgaaagt aagcctcact ggtggttcgt
     1561 ttggcttatt taacccctca gtgtcaagta catttgcaag caacaaatgg cgatgggcaa
     1621 tatccgcttc gcacaacgag tcagcgggct ataccgagaa taccgactat aggtatacac
     1681 aaggttttgt tcaagcacaa tttcaagcta cggaacaaga tatatggaaa tggcaaatag
     1741 gcgtgcaaga caaagctttt ggagcaaatt cattttattc ggtaaattat ccgaatcaat
     1801 ttgaaaaaac aaaaaccctt atcagcagta tcagttggga taaacgcatt aattccctgt
     1861 tgtggcaatc aactgcctat tacagagggc atttcgatca gtttgaacta tttcgcaatt
     1921 atgaaaacgc cgcaacttgg tacacgtttc acaattatca cctcagtcat gtaagtggca
     1981 tcaactcaaa gctgtcgtgg tttactagtt taggaaaatc aagcatcggc atagagggtc
     2041 gcaacgaaca tattaacagc aatgtgttag gagaactgaa agcaacacca taagccattc
     2101 cgtttgccaa aaataccaat acgcagtttt tatacgaaaa aaaccgtacc aatgtgcaat
     2161 attttgccga acaaacaatt tacttacagg catttacagc ttctgttggt gtatcgggca
     2221 attataatac gcaatttggc aacaggtata gttacgggct taacacttcg tatgcattca
     2281 atacgtatgc tcaggtattt gccaatatca ataaatcgtt acgactacct acctttactg
     2341 atttatacta caaaagtgct acgcaagaga gtaacccgag cttaaaacct gaagaagcta
     2401 tcaccgcaga aataggagcc aaatacacca aacaggcatg gcaatggaat gcaaacgcat
     2461 tttaccgtat agccaacaat agtatcgact gggtacgctc gctcgaaaca gaaaaatgga
     2521 aaagcatgaa ccatactcgc attgatgcca tgggaggaga aatagcggca gcttatacag
     2581 gtggtttttg ggcaaaaaac atacaagcat cgtatgccta cgttctgctc aacaaagatg
     2641 cgggcgagct aatttcaaaa tatgctctcg actacctgca acacaaaatt aacctgcaat
     2701 atgtgcatgg aataagcaag caggtagatg ccagttgggt agtaagctac caagaccgta
     2761 aaggaaatta tgtaaacaaa aatggaaaca aagtagctta tactccattt gccaccatcg
     2821 acactcgcat caactggcaa caaaataaca caaaaatgta tgccgaaata tccaatatcc
     2881 tcaaccaaca ttattacgac tatggcggcg tgcccatcgc tggacgtatg ttgaaagttg
     2941 gtattgcggt gcagttgtaa cacattcact tattctttct ctaaaccttg ctcttgaaaa
     3001 aa
//
LOCUS       DAOSMY010000194         2996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_32922_length_2996_cov_3.140428, whole genome shotgun sequence.
ACCESSION   DAOSMY010000194 DAOSMY010000000
VERSION     DAOSMY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32922_length_2996_cov_3.140428"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLD76_04985"
     CDS             complement(<1..265)
                     /locus_tag="PLD76_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU27390.1"
                     /translation="MKKNISKLLLKLMGWKTTASIPSIKKLVICVAPHTSNWDFIIGK
                     LFYTALGGKASFLIKQEWLKPPIGKLLINMGAIGVNRGKNTSLT"
     gene            complement(351..638)
                     /locus_tag="PLD76_04990"
     CDS             complement(351..638)
                     /locus_tag="PLD76_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27391.1"
                     /translation="MANKRNLKKDIQSIFSELLVECYTLSYIFPQIDIAKVDAIATSI
                     IEKSDNFVKRAGKPDGKDNPAIVKAYYKKLYKDVANTVSVFIEDINTLKSK"
     gene            795..2192
                     /locus_tag="PLD76_04995"
     CDS             795..2192
                     /locus_tag="PLD76_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27392.1"
                     /translation="MVWIGFSSCEDMMFSTNPNQRIHISADTVSFDTLFTGVGSTTKI
                     FNIYNPSKNRIKIDAIELVGATHYSLNINGVLTDALHDISLKANDSMRIFVQAYVDPS
                     DQFTPFIVKDSIIIRSNTHTYKVVLQAYGQDAYRVSKRQIMADTTWLAEKPYLILDTL
                     TIAQGATLTLSEGVSLYFVKHASLQVYGTLKAKGIQSKPVVFRGDRMDNMFDNLPYDK
                     VPNQWQGIRFRKGSAHNELNYVVVKNTNQGIVLDSTSLDVLALAISNSIINNSATNLI
                     TASHTVMHISNSVVYNAGQSCLVLQGGKYNVVHSTIANYFSFPWVGRKATSVLLSNYI
                     NLGSQAMPVPLLEANFINSIVYGSWSNEVSVSSSYLNQKIEEPLVYVFKNSVIKISPA
                     TINDTNFLEIIANQDPKFNATKPYNYDFTLQSSSPALGNADNLVAAEYPIDMLGRQRL
                     FNDKADIGAYVIIAE"
     gene            2195..>2996
                     /locus_tag="PLD76_05000"
     CDS             2195..>2996
                     /locus_tag="PLD76_05000"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27393.1"
                     /translation="MKKSIFLYFLLSMYWLTQWLIVPCYAEIPFRVMCYNVENAFDCE
                     NDSLTNDDDFTPQGAYHWTPKKFKLKISNIAKVITAVGQFTPPSIVGLCEIENESVLK
                     SLVYFSPLRNLNYKYVHYDSPDKRGIDVALLYRPEQFRVLSSAPIGVSFASAPFSTTR
                     DILYVKGIAMYTDTLHIFVCHFPSRLGGELESEDRRIEVSRILRKQVDSLFLASPTST
                     MLLMGDFNDYPTNTSLFSILQAQDPQNAIIRSYGLYNLYYQYHNKPNIG"
BASE COUNT          894 a          504 c          537 g         1061 t
ORIGIN      
        1 ctgtgagcga agtgtttttc ccccggttaa cgccaatggc tcccatattg attagtaatt
       61 ttcctatagg aggtttcagc cattcttgtt tgataagaaa agaagctttt ccccctagag
      121 ctgtataaaa aagttttcca ataataaaat cccaattact agtatgtgga gcaacgcaaa
      181 tcactaattt tttgatagag ggaatgctgg ctgttgtttt ccatcccatt aattttaaca
      241 acaatttgct gatatttttt ttcataaaat aaaatgcttt attcgtttgt ttttttctct
      301 ggtaatttaa gtgtcaagtt tgctgctaac gaatagtaaa atatgcttat ttatttgctt
      361 tttaacgtgt taatatcttc gataaaaaca gatactgtat tggctacatc cttgtatagc
      421 tttttgtaat aggcttttac gatagctgga ttgtctttgc catctggttt tcctgcacgt
      481 tttacaaaat tatcactttt ttcaatgata gaagttgcta ttgcatcaac tttggcaatg
      541 tcaatttgtg gaaatatata agataaggtg taacactcta ctaataattc agaaaaaatg
      601 gattgaatgt cttttttaag attacgcttg tttgccatat gaaaggagtt tttttcgtta
      661 ataattccac aaatttacaa gattattgca ataaaacaat ctattagtgt aattttgtac
      721 acgaattgct atatcattaa gaataacgta tgtatcgtca acgaactttt ttctcgtttt
      781 tttttcttat ttgtatggtt tggataggtt tttcatcatg cgaagatatg atgttttcta
      841 caaatcccaa tcaacgtata catatttctg cggataccgt ttcttttgat acacttttta
      901 cgggagttgg atctacaacc aaaatattca acatatataa tccatctaaa aatagaatta
      961 aaatcgatgc tattgagtta gtaggagcta ctcattattc tctaaatatt aatggtgtac
     1021 taacagatgc tttgcatgat atttcactca aagcaaatga tagtatgcgt atttttgtgc
     1081 aggcttatgt cgatccatct gaccaattta cgccctttat agtaaaagat tccatcatta
     1141 ttcgttcaaa tacgcatacg tataaggtag tgttgcaagc atacggacag gatgcatatc
     1201 gtgtttctaa acgtcagata atggcagata ctacctggct agccgaaaaa ccatatctca
     1261 tactcgatac attgacaatt gctcagggtg caacattaac cttgtcggaa ggggtttctc
     1321 tttattttgt taagcatgcc agtttacaag tatatggtac cctaaaagcg aaaggaatac
     1381 aatccaaacc tgttgttttt agaggggata gaatggataa tatgtttgat aatttacctt
     1441 acgataaagt gcccaatcag tggcaaggta tccgttttcg gaaagggagt gcacataacg
     1501 aattaaatta tgtcgttgtc aaaaatacaa atcaagggat tgttcttgac tctacttcct
     1561 tagatgtgtt ggctcttgcg atttctaatt cgattattaa taattcagct actaatttaa
     1621 ttacggcatc gcataccgtt atgcatataa gcaacagtgt agtatacaat gcagggcaat
     1681 cttgtttggt gttacagggt ggaaaatata atgtggtgca ttctactatt gccaattatt
     1741 tttccttccc ttgggttggt cgtaaagcaa cttcggtgct gctatctaat tatataaacc
     1801 ttggttctca agctatgcca gttccattac tcgaagccaa ttttatcaat agtatagtat
     1861 atggtagttg gtcgaatgaa gtatctgtca gttcctcgta tctaaatcaa aaaattgaag
     1921 aacctttagt gtatgtattt aaaaattcag taatcaaaat tagtcctgcc actatcaatg
     1981 ataccaattt tttggaaata atagctaacc aagatcctaa gtttaacgcg acgaaaccat
     2041 acaattatga ttttaccttg caaagctcct cgcctgcttt gggtaatgct gataatctgg
     2101 tagctgctga atatcctatt gatatgcttg gtcgtcaaag gttgtttaat gacaaagctg
     2161 atataggtgc gtatgttata atcgctgaat aggcatgaag aagagcattt ttttatactt
     2221 cctgctaagt atgtattggc ttactcaatg gctgattgtt ccctgttatg cagagatacc
     2281 gtttcgtgta atgtgttata atgtcgaaaa tgcattcgac tgcgagaatg attctcttac
     2341 caatgatgat gattttacac cacagggagc ctatcattgg actcccaaaa agtttaaact
     2401 aaaaatttcc aatattgcta aagttataac ggctgttgga caatttactc caccatcgat
     2461 tgtgggtttg tgcgagatag aaaacgaatc ggtattaaaa tcgttggtgt atttttctcc
     2521 tttacgaaat ttgaattata aatatgttca ttacgattcg cctgataagc gtggtataga
     2581 tgttgctctt ttgtatcgtc cagagcagtt tagagtgcta tcttctgcac ctattggggt
     2641 ttcgtttgct agtgcacctt tttctactac ccgagatata ttgtatgtaa aaggtatagc
     2701 tatgtatacc gatacattac atatttttgt gtgccatttc ccttcccgat taggtggtga
     2761 actagagtcg gaagacaggc gaatagaggt atctaggatt ttacgtaagc aagtagatag
     2821 tctgtttttg gcttctccca cttctactat gctactaatg ggagatttta atgattatcc
     2881 caccaacaca agtttatttt ctatattgca agcccaagat ccacaaaatg caattattcg
     2941 ttcgtacggt ttgtataact tatattatca gtatcataac aaaccgaata taggta
//
LOCUS       DAOSMY010000195         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33011_length_2990_cov_2.863714, whole genome shotgun sequence.
ACCESSION   DAOSMY010000195 DAOSMY010000000
VERSION     DAOSMY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33011_length_2990_cov_2.863714"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PLD76_05005"
     CDS             <1..1671
                     /locus_tag="PLD76_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU27394.1"
                     /translation="ALESDLSTTTVPEFIQKVVRPINAQDGDSLPVSSFTGREDGTWN
                     QGTAKYEKRGVAASVPVWDAENCIQCNQCAYVCPHAAIRPFVLDEAEQAKAPFKEMLK
                     ANGKQFAGMAFRIQVDVLDCMGCNNCVDVCPGNKGNKALELVAIETQYPNQDNWDFCM
                     NEVSSKQHLVDVKANVKNSQLATPLFESSGACAGCGETPYVKLVTQLYGERQMVANAT
                     GCSSIYSASAPSTPYTTTPEGRGPAWANSLFEDNAEFGLGMVFAEEHMRARIVRIMNE
                     AIASDCCPNEIKDLFKEWLENRLDEQKSKELQKQITPLVKASTCSHCQQIATMTQYLV
                     KQSQWIIGGDGWAYDIGFGGLDHVIASGKNVNILVLDTEIYSNTGGQASKSTPVGAVA
                     KFAASGKKVRKKDLGLIAATYGYVYVAQVAMGANQAQTLKAIREAEAYDGPSLVIAYS
                     PCISHGIAAGMGKAQEEQKRAVECGYWHLYRYNPDMELEGKNPMQLDSKEPDWSKFQD
                     FLKGEVRYTSLLKQFPQEAKELFEAAENNAKWRYNSYKRMANQQWNVE"
     gene            1819..1995
                     /locus_tag="PLD76_05010"
     CDS             1819..1995
                     /locus_tag="PLD76_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU27395.1"
                     /translation="MDSVAKVLDVIKKASEPVNATKVVELSGLDKKEVDKAMKTLKDA
                     GHIVSPKRCYWSAK"
     gene            2019..2921
                     /locus_tag="PLD76_05015"
     CDS             2019..2921
                     /locus_tag="PLD76_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU27396.1"
                     /translation="MSHHHHHHHSPIVESSFSLGKAFFFAIVLNVLFVVAEFATGFIY
                     NSIGLISDAAHNLGDVASLLLAWLAFKLAKVSTKTNYTYGFKKSTILVALTNAIILLF
                     TVGVIIVKSISKFYYPSPVEGGVIAWVAGVGVVINLATAFLFIRDKEKDLNVKGAYLH
                     MMADAIVSVGVVVSGITILFTGWNFLDPIIGLLIAVVILFSTWNLLRDSIRLSLDGVP
                     SSVDIEEIKSVILQHPAVESIHHLHIWAISTTETALTVHVVLRNLETMEISKQELKKI
                     LAAHLISHATIEIETADGMCGELC"
BASE COUNT          986 a          429 c          639 g          936 t
ORIGIN      
        1 gctttggaaa gtgatttgtc tactactact gtgccagaat ttattcaaaa agttgttcgt
       61 ccaattaatg cacaagatgg agatagtctt ccagtttcgt catttacagg aagagaagat
      121 ggtacatgga atcaaggtac agcaaaatac gaaaaacgtg gtgtggcagc ctctgtacct
      181 gtatgggatg cggaaaattg tattcaatgt aatcaatgtg cttatgtgtg tcctcatgcc
      241 gctattcgtc cgtttgtgtt agatgaagca gagcaagcta aagcaccatt taaagagatg
      301 cttaaagcaa atggtaagca atttgcaggt atggcatttc gtatacaagt tgatgtatta
      361 gactgtatgg gatgtaataa ctgtgtggat gtgtgcccag gaaataaagg aaacaaggct
      421 cttgaattgg tagcgattga aacacaatat ccaaatcagg acaattggga tttctgtatg
      481 aacgaagtaa gttcgaaaca acatttggtg gatgtaaaag ccaacgtaaa gaattcacaa
      541 ttagcaactc ctttatttga atcatctgga gcttgtgctg gttgtggcga aactccttat
      601 gtaaaattgg taacacaatt gtatggagaa agacaaatgg tggcaaatgc tacaggttgt
      661 agctctattt attctgcatc agcgccttct actccttata caacaacacc agaaggaaga
      721 ggtccagcat gggcaaattc attgtttgaa gataatgctg aatttggttt aggtatggtg
      781 tttgcagaag aacatatgcg tgcacgtatc gtacgtatta tgaatgaagc gatagctagc
      841 gattgttgtc caaatgaaat taaagattta tttaaagaat ggcttgaaaa tagattagat
      901 gaacaaaagt ctaaagaatt acaaaaacaa ataactccat tagttaaagc aagtacatgt
      961 tctcattgtc aacaaattgc tacaatgaca cagtatttag ttaaacaatc gcaatggatt
     1021 ataggtggtg atggttgggc ttacgatatc ggttttggtg gattagatca cgtgattgca
     1081 tcaggaaaaa atgttaacat tttggtgctt gataccgaaa tttactctaa tactggtgga
     1141 caagcttcta aatcaactcc agttggagcg gtagctaaat ttgctgcatc aggtaaaaaa
     1201 gtacgtaaaa aagatttagg tttaatagca gccacttatg gttatgtgta tgtagcacaa
     1261 gtagccatgg gagctaatca ggcacaaacc ttgaaagcta ttagagaggc agaggcatat
     1321 gatggaccat ctcttgtaat tgcgtattct ccatgtatta gtcacggtat tgctgcaggt
     1381 atgggcaaag ctcaagaaga gcaaaaacgt gctgttgaat gtggttactg gcacttatat
     1441 cgatataatc cagatatgga acttgagggc aaaaacccaa tgcaattaga ttctaaagaa
     1501 ccagattgga gcaaattcca agatttctta aaaggagagg ttcgttacac atcattgttg
     1561 aaacaattcc ctcaggaagc aaaagaatta tttgaggctg ctgaaaataa tgctaaatgg
     1621 cgttataatt catataaacg tatggcaaat caacaatgga atgttgagta actagaaaaa
     1681 tgattgtaaa atgcaaggag agtatatgga gtaaaaaaca tatactctcc ttttttatta
     1741 aaatatctaa aaataaacat agctactaca tatatttttg taattttgat tatttattaa
     1801 tttaaaaata gatagaatat ggattcagta gcaaaagtat tggatgtgat aaaaaaagca
     1861 tcagaacctg taaatgcaac aaaagtagta gaattatctg gtttagataa gaaagaagta
     1921 gacaaggcaa tgaagacatt aaaagatgct gggcatatcg tgtctccaaa gcgttgttat
     1981 tggagtgcta aatagctcat tcataacatt acaatagtat gtctcaccac caccaccatc
     2041 atcattcacc aatagtagag tcttcttttt cgttagggaa agctttcttt tttgccattg
     2101 tgctgaatgt attatttgta gttgctgaat ttgccactgg ttttatctat aattcaatag
     2161 gattaatatc agatgcagca cataatttgg gtgatgtggc tagtttgttg ttagcttggt
     2221 tggcatttaa attggcaaag gtgtcaacaa aaacaaacta tacatatggt tttaaaaaaa
     2281 gcaccatact cgtggcactt acgaatgcta taattttgtt gtttacggtg ggagttatta
     2341 ttgttaaaag catttcaaaa ttttattatc caagtcctgt agaaggaggt gtgattgcct
     2401 gggttgctgg agtaggggtt gttattaatt tagcaacagc ttttcttttt attcgcgata
     2461 aggaaaaaga tttgaatgta aagggagctt atttgcatat gatggcggat gctattgtat
     2521 ctgttggggt tgtcgtttcg gggatcacca ttctattcac tggatggaat tttcttgatc
     2581 ctataatagg tttgctgata gctgttgtta ttttattttc aacgtggaat ttattgcgtg
     2641 atagcatccg cttatcttta gatggagttc cgtctagcgt agatatagaa gaaattaaat
     2701 cggtgattct tcaacatcca gctgtggaaa gtattcatca tttgcatatt tgggcaataa
     2761 gtacaactga aactgctttg actgtgcatg ttgtgttacg taatttagaa acaatggaaa
     2821 ttagtaagca agagttaaag aaaatcttgg cagcacattt aattagtcat gcaacaatag
     2881 aaatagaaac tgcagatggt atgtgtggag aattatgtta aaccctttat tcttttacag
     2941 atattttaaa tataagtgag gttgaggtag ttaccttgta tctgtcatca
//
LOCUS       DAOSMY010000196         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33051_length_2987_cov_3.295362, whole genome shotgun sequence.
ACCESSION   DAOSMY010000196 DAOSMY010000000
VERSION     DAOSMY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33051_length_2987_cov_3.295362"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1316
                     /gene="serS"
                     /locus_tag="PLD76_05020"
     CDS             45..1316
                     /gene="serS"
                     /locus_tag="PLD76_05020"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU27397.1"
                     /translation="MLTTKFITENATEVIARLAKKHFDASLIVSEVISLDTLRKTTQT
                     KLDEVLAEMNAISKAIGDLYKQGKKQEADEAKEKTVVLKEKSKELSDILDSAEQKLHT
                     LLLQIPNLPHASVPEGKGADDNTIERSGGTIPSLPANALPHWELAKKYDLIDFELGVK
                     ISGAGFPVYKGKGAQLQRALINFFLSNASQAGYLEIQPPYVVNAASGYGTGQLPDKDG
                     QMYHATIDDLYLIPTAEVPVTNIYRDVILQEKELPIKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDKSYETLDEMVNYVQTLVEKLELPWRILRLCGGDMSFTSA
                     LTFDFEVFSAAQNRWLEVSSVSNFESFQANRLKCRYKGDDKKTSLCHTLNGSALALPR
                     IVAALLENNQTEEGIVIPKALVPFTGFTIIS"
     gene            1488..2561
                     /gene="corA"
                     /locus_tag="PLD76_05025"
     CDS             1488..2561
                     /gene="corA"
                     /locus_tag="PLD76_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOU27398.1"
                     /translation="METLKPLRSVSILNRKKNDPSNYIFTGNSSTKNINIQLFTYNEE
                     ECVENKIMLLDSIPTFEKNKKNYWLNIHGLNNPENIASICEQQGIHNLVIQDILDVNQ
                     RPKFQEFEHFSFLTLKSIVPSEKNIITEQISFVFGTNFLLSFQERKADFFEHLRYRLR
                     ESKGKLRERGADYLLYAMLESTLDNYFKTINKLDEEVEKLNFSDTTKEPSPNALAIIE
                     GHKKFVHYIKKSILPIKEFTQIVERGDCNYIEQRHVKYFLEIKDLCLTLIDSCDMILA
                     SLESATNLFFSVQGHRMNQVMKTLTIVATIFMPLTFIAGIYGMNFVHMPELEWKYGYY
                     GIWGIMIVLFCAMVLYFRAKKWF"
BASE COUNT         1044 a          418 c          523 g         1002 t
ORIGIN      
        1 aaagggtggg ttttttttta tttactttta taatagcaca acttatgctc actaccaagt
       61 ttattacaga aaatgccaca gaagttattg ctcgtttggc aaaaaaacat tttgatgcat
      121 cgttaatagt atctgaagta atttctttag atacattacg caaaacaaca caaacaaaat
      181 tagacgaagt attagccgaa atgaatgcta tctccaaagc aattggagat ttgtacaaac
      241 aaggcaaaaa acaggaggcg gatgaggcta aagaaaagac cgttgtgtta aaagaaaaaa
      301 gcaaagagtt aagtgatatt ttggatagtg ctgaacaaaa gttacataca ctattattac
      361 aaatacctaa tttgcctcat gcatctgtac ctgaaggaaa aggagctgac gataatacta
      421 ttgagcgtag tggtggcaca attccttctt taccagcaaa tgctttgcct cattgggaat
      481 tagcaaaaaa atacgatctg attgactttg agttaggagt taaaatatca ggagcaggtt
      541 tccctgttta taaaggaaaa ggggcacaat tgcaacgtgc tttgataaat ttctttttat
      601 ctaatgcctc tcaagcaggt tatctcgaaa tacaacctcc gtatgtggtg aatgcagcat
      661 cgggttatgg aactggtcaa ttacctgata aggatgggca aatgtatcat gcaacgattg
      721 atgatttgta tttaattcca actgcagaag tgccagtaac caatatttat cgcgatgtga
      781 ttttgcaaga aaaggaattg ccaataaaaa atacggcata ttcagcttgc tttagaagag
      841 aagcggggtc ttatggaaaa gatgtgcgtg gattaaatcg cttacaccaa tttgataagg
      901 ttgaaatagt gcaaattcaa catccagata aatcctatga aacattggat gaaatggtta
      961 attatgtaca aacattggtt gaaaaacttg aattgccttg gcgtatatta cgcctttgtg
     1021 gtggagatat gagttttact tcggcattaa ctttcgactt tgaggtattt tcggctgctc
     1081 aaaaccgttg gttagaagta agttctgtat ccaactttga aagttttcaa gcaaatagat
     1141 taaaatgtag gtacaaagga gatgataaaa aaactagttt atgtcataca cttaatggta
     1201 gtgcacttgc gttgcctcgt atcgtagctg cattgttaga aaataaccaa actgaagagg
     1261 gtattgttat tccaaaagcc ttagttccat ttactggttt tactattatt tcgtaacaaa
     1321 tactcatttc ttactgtata tttaaaatag gaggcttttt gtttcctatt ttttttgttc
     1381 agacatttaa tggcaaattt acgatgaaaa tataaaaaat gtagttatct tcgaagtcta
     1441 taagtaaaaa atgtatgtaa agaatatata agcaactagc aaaaaacatg gaaactctca
     1501 aaccattacg atcggtttct attttaaata gaaagaaaaa cgacccaagt aactatattt
     1561 ttacaggaaa ttcgagtact aaaaatatta atattcagtt atttacttat aatgaggaag
     1621 aatgtgtaga aaataaaatt atgctactag atagtattcc gacgtttgaa aaaaataaaa
     1681 aaaattactg gttaaatata catggattaa ataatccaga aaatattgcg tctatttgtg
     1741 aacaacaagg gattcataac ctagttattc aggatatttt agatgtaaat caacgaccaa
     1801 aatttcagga atttgaacat ttttcttttt taacattaaa atcaatagtt ccttcggaga
     1861 aaaatatcat aactgagcaa attagctttg tatttggtac caactttcta ttgtcttttc
     1921 aagagagaaa agcggacttt tttgagcatc ttcgatatag actcagagaa agtaaaggta
     1981 aattacgaga acgaggtgca gattatttgc tttatgctat gctagaatca actctcgata
     2041 actacttcaa aactattaat aagctagatg aggaagtgga aaaattaaat ttttcggata
     2101 ccacaaaaga accatccccc aatgctcttg ctataattga aggacataaa aaatttgttc
     2161 attatataaa aaaatctata ttacctataa aagaatttac acaaattgtt gaacgtggtg
     2221 attgtaacta tatagaacaa aggcacgtaa aatattttct tgaaattaaa gatttgtgtt
     2281 taacattgat tgatagttgc gatatgattc ttgcttcgct tgaaagtgca acaaacttat
     2341 ttttctcagt tcaggggcat cgtatgaatc aagttatgaa aaccttaacc atagtggcta
     2401 caatctttat gccacttaca tttattgcag gtatttatgg tatgaatttt gtgcatatgc
     2461 cagaacttga gtggaagtac ggatattatg gcatttgggg tattatgatt gttttatttt
     2521 gtgctatggt actctatttt agagctaaaa agtggtttta gatatatctt tttatactta
     2581 cgtaatttgt tattcttcat tcgcgtattt taaattttat cttcttaaaa acttttgttt
     2641 tttgtttctt ttttaatttt taagggttaa tttacacatt ttatactttt ttgtgtattg
     2701 taagtatttg ataatcaatg atagattttt tttgttgata tttttatcta ctttatcccg
     2761 ttcacaaata aaagatttgt ttaactttgc catgtgaatt aaaatttcaa ttattcatta
     2821 aaaagtaaca agtatgtcac tacaaaaaga cattttagaa ttacgtgaga aaaaagccat
     2881 ctatgaaaaa ggtggtggcg acacagctat cgaaaaacaa atggctatgg ggaaattaac
     2941 agcccgccaa cgtattttag ctctattgga tgaagattct tttcaag
//
LOCUS       DAOSMY010000197         2986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33065_length_2986_cov_3.770044, whole genome shotgun sequence.
ACCESSION   DAOSMY010000197 DAOSMY010000000
VERSION     DAOSMY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33065_length_2986_cov_3.770044"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..982)
                     /locus_tag="PLD76_05030"
     CDS             complement(26..982)
                     /locus_tag="PLD76_05030"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HOU27399.1"
                     /translation="MRTYTNVKDLGNLQQALNEAFEVKNNPYKWDTLGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTMVLDINQGAWKLETERGVIMDGDTTEHILEAIPVMGS
                     YCDIIGVRSFARFENKEFDCQDTILKQFIQYSGKPVFSMEGAIGHPLQAFADLITIEQ
                     YKKTDRPKVVLTWAPHPKALPQAVPNSFADFMNEANVDFVITHPKGYELDEKFVRGAR
                     VEYDQRKAFEGADFIYAKNWSAFQDPHYGQILSKDRDWTVDTEKMALTNNAYFMHCLP
                     VRRNMIVTDDVIESPQSIVIPQAANREVSAQTVIKRMLEALK"
     gene            1147..2751
                     /locus_tag="PLD76_05035"
     CDS             1147..2751
                     /locus_tag="PLD76_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27400.1"
                     /translation="MKYLHVSIFCLLFSLTVTPLFSQKSPFNTTAAELFADGKSFFYN
                     NNYAASARYFEEFLQFDQRDNDEMVREAEYYIARSAFELRKNDAQIKLEAYQTKYPYA
                     TDVELTNLYIGILEYESGRFKQAIRRFESIKTDRLRESDLVKLQFYKGYAYHQQENFE
                     KSRFEFQQVLRRGTNTPYYDAATYYFAYAEQRLGNFDSALTHFLRVENHPEFHTSAPF
                     FIAQLYYQQGNCDKVLNYGKEIIKKYPKNNRRNEVLRMMGECSFLAKDYSSSIHYLTQ
                     YEQQVKRISREDFYMLGIAHYNVGEYEKAIANLSKTTNREDLLAQNSYLYLGMGYVKL
                     NDKKNARLAFETASRFTFDKGIQEEALYNFAVVSYEQSFSPFNESVVAFERFLTEFPS
                     SKRKDKVYEYLANSYLTTKNYGEAYRSIQKLKTDNKKIKEAEERILFNMGIVAVANNN
                     YQQATSHFEKILKGKSYNADFKTRSFYWYGESLLNTGYYTQAEKNFQTFLQSPKAQKL
                     AEYDIAHYSMGYTYYLLKNYRCCNMV"
     gene            2786..>2986
                     /locus_tag="PLD76_05040"
     CDS             2786..>2986
                     /locus_tag="PLD76_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27401.1"
                     /translation="MVLDANNRIGDSYYQNRQFAEAKRYYNQAIATGAQLPGADYATF
                     QKGFVAGLERNHNEKISTLRNLI"
BASE COUNT         1007 a          536 c          513 g          930 t
ORIGIN      
        1 ttgtactgaa aggttcatac taagcttatt tcaatgcctc taacatacgc ttaataacag
       61 tttgtgcaga cacctcccta ttagcggctt gagggattac aattgattga gggctttcaa
      121 ttacatcgtc cgtaacaatc atattgcgtc ttacgggcag acagtgcata aaataagcat
      181 tattggtaag tgccatcttt tcggtatcta ctgtccaatc tctatcttta gacaagattt
      241 gcccataatg tggatcttga aaagctgacc agtttttagc atagataaaa tcagcacctt
      301 caaacgcttt gcgttgatcg tattctacac gagcacctct tacaaacttt tcatcaagct
      361 catacccttt ggggtgtgta ataacaaaat caacattggc ttcgttcata aaatcggcaa
      421 acgaattggg cacagcttga ggcaaagcct tagggtgagg agcccaggtg agcacaactt
      481 ttggtctatc tgtttttttg tattgttcaa tggtaattaa atcggcaaaa gcctgcaatg
      541 gatgcccgat agctccctcc atgctaaata cgggtttgcc agaatattgg ataaattgtt
      601 ttagaatggt atcttgacaa tcaaactctt tgttttcaaa acgagcaaac gaacgaacac
      661 cgataatatc gcagtaagaa cccataacgg gaatagcttc taagatgtgc tctgtagtgt
      721 cgccatccat aattacaccg cgttctgttt ctaatttcca agctccttga ttaatgtcga
      781 gtaccatggt gttcatacct aaattcatgc ctgctttttg tgtacttaaa cgggtacgta
      841 gactagaatt aaaaaaaacc atcaacaaag ttttgttttt tcctagtgtg tcccatttat
      901 aaggattatt ttttacttca aaagcctcat ttaatgcttg ttgcaaattt cctaaatctt
      961 ttacgttggt gtaagttctc ataatgaatg acgaattgat taaaacacaa aggtacacaa
     1021 aaaacgtaaa ttgattgagg atttttatgt ttttaaattc aaatataatt tatacctttg
     1081 caagtaataa tctgattttt tattcgttct agcgttatac attgattcag aaaaaatcga
     1141 acacttatga aatacttaca cgtttccatt ttttgtctac tatttagttt aaccgttact
     1201 cctcttttta gtcaaaaatc accttttaac actactgctg ctgaactttt tgcagacggt
     1261 aagtcctttt tctacaataa taattatgct gcatcggcac gatattttga agagttttta
     1321 caatttgacc aacgagacaa tgacgaaatg gtgcgtgaag cggagtatta cattgctcgt
     1381 tcagcatttg aacttagaaa aaatgacgca caaattaaac ttgaggcata tcagacaaaa
     1441 tacccttatg caacagacgt agaacttacg aatctatata ttggcatttt agagtacgaa
     1501 tctggtagat ttaagcaagc cattcgtcgt tttgagagca taaaaacaga tagattacgc
     1561 gaatcggatc tagttaaatt gcaattctac aaaggctatg cataccatca acaagaaaat
     1621 tttgaaaaat ctcgttttga atttcagcaa gtactccgca gaggcacgaa tacgccttat
     1681 tacgatgcag ctacctatta ttttgcttat gcagagcaac gattaggcaa ttttgatagt
     1741 gctcttactc attttttacg cgtcgaaaat catcctgaat ttcatactag tgctccattt
     1801 tttattgccc aactatatta tcagcaagga aattgtgata aagtattaaa ttacggaaaa
     1861 gaaattataa agaaataccc taaaaacaat cgacgtaatg aagtattacg gatgatgggc
     1921 gaatgtagtt ttcttgccaa agattatagc tcaagcatac attaccttac tcagtatgag
     1981 caacaagtaa aacgaatttc gcgtgaagat ttttatatgt tagggatagc tcattacaac
     2041 gtgggtgaat atgaaaaagc tattgcaaac ttgtctaaaa caacaaacag agaagattta
     2101 ttagcacaaa atagttacct atacttggga atgggatatg tcaagctaaa cgataagaaa
     2161 aatgcacgac tagcttttga aacagcttct cgttttacct ttgataaagg catacaagaa
     2221 gaagccttgt ataattttgc tgtagttagt tacgaacagt cattttctcc atttaatgaa
     2281 tctgtagttg cttttgagcg ttttttaacc gaattcccct catccaaacg caaagacaaa
     2341 gtatatgaat acctagcaaa cagctattta actacaaaaa attatggaga agcatatcgt
     2401 tctattcaga aactgaaaac tgacaataaa aaaattaaag aagctgaaga gcgtatttta
     2461 ttcaatatgg gtatagtggc tgttgccaat aacaattacc aacaagcaac atcgcatttt
     2521 gagaaaatat tgaagggtaa atcatacaat gcggacttta aaactcgttc attttactgg
     2581 tatggagaaa gtttgttgaa tactggatat tatacccaag ctgaaaagaa ttttcaaacc
     2641 tttttacaat cacctaaagc acaaaaacta gcagaatacg atattgctca ttatagtatg
     2701 gggtacacct actatttact gaagaactac agatgctgta acatggttta aacgatacat
     2761 tgcattaaaa ccagacaaca acctcatggt gttggatgcc aataatcgta ttggcgattc
     2821 ttattaccaa aacagacaat ttgcagaggc aaaacgttat tataatcaag ccatagcaac
     2881 aggggctcaa ttacctggag ctgattatgc tacattccaa aaaggatttg ttgctggatt
     2941 agaacgaaat cataatgaga aaatatcaac cttacgaaat ttaatt
//
LOCUS       DAOSMY010000198         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33172_length_2977_cov_4.341889, whole genome shotgun sequence.
ACCESSION   DAOSMY010000198 DAOSMY010000000
VERSION     DAOSMY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33172_length_2977_cov_4.341889"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..729
                     /locus_tag="PLD76_05045"
     CDS             106..729
                     /locus_tag="PLD76_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOU27402.1"
                     /translation="MVQSEKSICQQCVIDLPKTEFHLLQNNPVEKLFWGKIHVEFASA
                     FFFFQKGSVAQKLLHNLKYKGNKEIGIELGKHYATELVRSSLIDTIDYIVPVPLHKNK
                     LKKRGYNQSEMIAIGLSQVLSIPVETDTLVRAIENPTQTKKGVFERWENTYGVFTITN
                     TQQFANKHILLVDDVLTTGSTIEACVQAIKQAPNAKVSFISLALASN"
     gene            742..1476
                     /locus_tag="PLD76_05050"
     CDS             742..1476
                     /locus_tag="PLD76_05050"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU27403.1"
                     /translation="MIETIHIHETTSTNDLLNQLMREKSLEEGSMVYTDFQTLGRGVS
                     NNVWESEKGENLLCSILLKPSFIPIKQQFIISQVVSLALTDFLLPYDTEFTIKWPNDI
                     YWKDNKIGGILIENNLENGLLASCIIGIGLNINQKHFYSSAPNPISLIQITNTFHPIK
                     EIATSIRNAIFERYIHLLKGKTKELQQTYTDRLYRSKGLFRFQDKDGIFVASIFQVTP
                     DGYLELTKENGDRQRYAFKEVSFIIP"
     gene            complement(1466..1681)
                     /locus_tag="PLD76_05055"
     CDS             complement(1466..1681)
                     /locus_tag="PLD76_05055"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOU27404.1"
                     /translation="MSFLFGILFFILAIGLFVLVFFFSILRNVFRLFTGKQHPEKKEQ
                     ERMARKYVNKKITKQQGEYVDFEEIKE"
     gene            complement(1688..2482)
                     /gene="lgt"
                     /locus_tag="PLD76_05060"
     CDS             complement(1688..2482)
                     /gene="lgt"
                     /locus_tag="PLD76_05060"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU27405.1"
                     /translation="MALATIIWDVNPEAFSIGFITVRWYGLLYAMGFLLGITILGKIF
                     KRENCPEDWADKVFIYMVLAVVIGARLGHVFFYAWDYYKDNLLEIVMIWHGGLASHGG
                     AIAMLIAAWIFSKKVSKKSFLWLGDRLFIPSAMVAGLIRIGNLMNSEIYGHATDLPWG
                     FVFVRDGGTMPMHPTQIYEALAYFALFAFMMYLYWVKDAQKREGLLTGVGFTGIFVAR
                     FFIEFVKNNQEAFEDDLMLNMGQYLSIPFIAIGIWLIIRALRSNKI"
     gene            complement(2514..>2977)
                     /locus_tag="PLD76_05065"
     CDS             complement(2514..>2977)
                     /locus_tag="PLD76_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOU27406.1"
                     /translation="ETQAKEIVKSFQKSILLHTIDEAWKEHLRELDELRNSVQNASYE
                     QKDPLLIYKLESFGLFKKMIDAINYKAIAILMRGQIPVKEGETLREAKEERRQDFSKY
                     NTQKSDIMNAGQQDTREKIHKEPVRVEKKVGRNDLCPCGSGKKYKSCCGKV"
BASE COUNT         1031 a          593 c          416 g          937 t
ORIGIN      
        1 gtataatttt tcctttacca ttattctctt gcatatctac aacactatat ctcaggattt
       61 atatccttaa tttacccaca agtttgtatc gtttgtggaa accacttggt acaatcagag
      121 aaaagcatat gccaacaatg tgtcatagat cttccaaaaa cagaatttca tctactacaa
      181 aacaatcctg ttgagaaact attttgggga aaaatacatg tggagtttgc atcggctttt
      241 ttcttttttc agaaaggaag tgtggcacaa aagttactgc ataatttaaa gtacaaaggg
      301 aataaagaaa tagggataga gcttggcaaa cattatgcaa ccgaattagt gaggtctagc
      361 ttaattgata ccattgatta tattgttccc gttcctttac ataaaaacaa actaaaaaaa
      421 agagggtata accaaagtga aatgattgct atcggattat cacaagtgct ctctattcct
      481 gttgaaacag acacactggt aagagccatc gaaaatccaa cacaaacaaa gaaaggtgtg
      541 tttgaaagat gggaaaatac gtatggtgtt tttactataa ccaataccca acaatttgca
      601 aataaacata tcctactggt cgatgatgta ttaactacag gatctaccat agaagcctgt
      661 gtacaagcga ttaaacaagc cccaaatgcg aaagttagtt ttataagttt agctttagca
      721 tctaattaaa ctaaaccgct aatgattgaa accatacaca tacacgaaac aacctcaaca
      781 aatgatttac ttaatcaatt aatgagagaa aaatcactag aggaaggttc tatggtatat
      841 acagatttcc aaacacttgg aagaggcgtt tcgaataatg tatgggaatc agaaaaagga
      901 gaaaatttgc tttgtagtat attactaaaa ccatctttta ttcctattaa gcaacaattt
      961 attatttcgc aggtagtgtc attagctctt actgactttt tattgcctta tgatactgaa
     1021 tttactatca agtggcccaa tgatatttat tggaaagaca ataaaattgg aggtatttta
     1081 attgaaaaca atttagaaaa tggattatta gcgtcttgta tcattggtat tggtctgaat
     1141 atcaaccaaa aacatttcta ttcatctgca cctaatccaa tttcacttat acagattacc
     1201 aacacttttc atcctattaa ggagatagct accagcatac gaaatgctat ttttgaacgc
     1261 tatattcact tactcaaggg aaaaaccaaa gaattacaac aaacgtatac agacagatta
     1321 tatcgctcca aagggctgtt tcgttttcaa gataaagatg ggatttttgt cgcatctatt
     1381 ttccaagtaa cacctgatgg atatttggaa ttaacaaaag aaaacggaga tcgacaacgt
     1441 tatgctttta aagaagttag ttttattatt ccttaatctc ttcaaaatcg acatattcac
     1501 cctgttgctt tgtaattttc ttatttacat acttacgagc cattcgctct tgttcctttt
     1561 tctcaggatg ttgttttcct gtaaataaac gaaaaacatt acgtaatatg gaaaagaaaa
     1621 ataccaacac aaataaacca atagcgagta taaaaaataa tatcccaaat aaaaaactca
     1681 ttgcatctta tattttattc gaacgtaatg ctctaattat caaccatatt ccaatggcaa
     1741 taaatggaat acttaaatac tgacccatat ttaacattaa atcatcttca aaagcctctt
     1801 gattattctt tacaaattct ataaagaaac gagcaacaaa tattcccgta aatcctactc
     1861 cagtaagcaa gccttcacgt ttttgtgcat cctttaccca atataagtac atcataaaag
     1921 caaacagagc aaaataagct aaagcttcgt atatttgtgt tgggtgcatg ggcatagtac
     1981 ctccatcacg gacaaataca aaaccccatg gcaaatcggt agcatgacca tatatctcgg
     2041 aattcatcaa atttccaata cgaatcaaac ctgccaccat agccgaagga ataaacagtc
     2101 tgtctcctag ccacaaaaaa cttttcttac taaccttttt tgaaaaaatc caagcagcaa
     2161 tcaacatagc tatggcacca ccatgactgg ctaatccgcc atgccaaatc atcactatct
     2221 ctaacaaatt atctttgtaa taatcccacg cataaaaaaa cacatgtccc agcctagcac
     2281 caattaccac tgccaaaacc atgtaaataa acaccttatc agcccaatct tctggacaat
     2341 tttcacgctt aaatatttta cccaaaatag taatacccag caaaaacccc attgcataaa
     2401 gtaaaccata ccaacgcaca gttataaatc caatcgaaaa agcttcagga tttacatccc
     2461 atataatcgt agctagtgcc attctctgat gtttttaaat tattatactt gcattatact
     2521 ttgccacaac aactcttgta tttctttccg ctaccacaag gacatagatc attacgaccc
     2581 actttcttct caacacgcac tggttctttg tgtatttttt ctcgggtatc ttgttgacca
     2641 gcattcatta tatccgattt ttgcgtattg tatttactaa aatcttgtcg tcgctcttct
     2701 tttgcctctc ttaatgtttc tccttctttc accggaattt gaccacgcat caaaatagca
     2761 atcgctttgt aatttatagc atcaatcatt ttcttaaata gcccaaatga ctctagcttg
     2821 tagattaata atggatcttt ttgctcataa ctagcatttt gtactgagtt acgtaattca
     2881 tctaattcgc gtaaatgctc tttccaagct tcatctattg tatgcaacaa aatagatttt
     2941 tggaacgact tcacaatttc ttttgcttgt gtttcgt
//
LOCUS       DAOSMY010000199         2975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33205_length_2975_cov_3.392808, whole genome shotgun sequence.
ACCESSION   DAOSMY010000199 DAOSMY010000000
VERSION     DAOSMY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33205_length_2975_cov_3.392808"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLD76_05070"
     CDS             <1..768
                     /locus_tag="PLD76_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU27407.1"
                     /translation="ARLSLHQLVTDGRIHPARIEEVVAKVTKQIEEEIVEIGKRTTID
                     MGVHGLHPELVRLIGKMKYRSSYGQNLLQHSRETANLCAVMAAELGLNPKKARRAGLL
                     HDIGKVADEEPELPHALLGMKLAEKYKEKPDICNAIGAHHDEVEMQTLLAPIVQACDA
                     ISGARPGARREIVEAYIKRLNDLENIALSYPGVMKTYAIQAGRELRVIVGADKIDDQA
                     TERLSQEIAKRIQDEMTYPGQVKITVIREIRSVSVAK"
     gene            780..1178
                     /gene="ssrA"
                     /locus_tag="PLD76_05075"
     tmRNA           780..1178
                     /gene="ssrA"
                     /locus_tag="PLD76_05075"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1883..2332)
                     /locus_tag="PLD76_05080"
     CDS             complement(1883..2332)
                     /locus_tag="PLD76_05080"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOU27408.1"
                     /translation="MKLQAICPISSQQIDERIPRINGAITVLLLVLFALTQHYFIILF
                     LVADFFARAIEKPDYSILAKLSKAVLARFSTTGKKTNAGPKIFAARIGLVFSLSIAIF
                     SLFGVDSVATVLVVIFGICAFLEAAFNFCVACKIYPFVYKFTHLNEM"
     gene            complement(2500..>2975)
                     /locus_tag="PLD76_05085"
     CDS             complement(2500..>2975)
                     /locus_tag="PLD76_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27409.1"
                     /translation="SGEIYTVYRVLDIEDGYLTFKRNKDSNISTIDASLVKTIGFQPK
                     QLRDATDAYDKAYIIEGSFYEKNKKLSESAYNDAWIQHNLNRFYTQKRCAQICYGLSL
                     ATSILYYTGSFDKELLYVSAGLSIAALAIDFHSYKWIKRASIKPSLQSLSVQLEF"
BASE COUNT          994 a          539 c          583 g          859 t
ORIGIN      
        1 gcacgtttat cgttacacca attggtaaca gatggacgca ttcaccctgc tcgtatcgaa
       61 gaggtagtag ctaaggtaac caaacaaatt gaagaagaaa tagtagaaat tggtaaacgt
      121 accaccattg atatgggtgt gcatggattg catcctgaat tggttcgctt gattggtaaa
      181 atgaaatacc gttcgtctta cggacaaaac ttattgcaac actcgcgcga aacggctaat
      241 ttatgtgccg ttatggcagc cgaattgggc ttaaatccga aaaaagcacg tagagcagga
      301 cttttgcacg atataggcaa agtggcagac gaggaaccag aattaccaca tgcactttta
      361 ggcatgaaac ttgcagagaa atacaaagaa aaacccgata tttgcaatgc cattggagct
      421 caccacgacg aggtagaaat gcaaacccta ctcgctccta ttgtgcaagc gtgtgatgct
      481 atttcgggtg ctcgtccagg tgctagacgc gaaattgtag aagcgtatat caaacgctta
      541 aacgacttag aaaatattgc cctatcgtat ccaggtgtaa tgaaaaccta cgccatacaa
      601 gcaggtagag agctacgtgt aattgtaggc gcagataaaa ttgatgacca agcaacagaa
      661 agactttcgc aagaaattgc gaaacgcatt caagacgaaa tgacctaccc tggtcaagtg
      721 aaaattaccg ttatcaggga aatcagatcg gtaagtgttg ctaagtagta cataacattg
      781 ggcttgactg gaattgacag cgagtaaaat tggtatgtaa gcatgcagag ctttgtatga
      841 gtagctcttt aatctgaaca tacaacaatc taattggcga aaataactac gctcttgctg
      901 cttaatcgaa taacagtaga ttagcttaat ttcggtacaa ggtgctgaaa caagacatct
      961 ctcaaatgcc cactccttga ggcggtttga tatagaggtg ctaactaaat cagggatagc
     1021 ttatcatcgc ctcgtatggt aagtgaaaat aaagaggata aggttgtagt tggtggtttc
     1081 ggtcttgcta caacacgaaa ataaaagcga aaataagcat gtagaaagcg tattaatttc
     1141 ttgtttggac gagggttcga ctccctccag gtccaccaac taaacaaaac cactgataat
     1201 ctatttatta tcagtggttt tgttattttt tatgtttgtg tttacgttac aataagttgt
     1261 ggttcattcg gaaaaattgt attttgataa ataaggtgat aatcttacct ttatgatagt
     1321 agtaggattt tcaagccact ttacatgata gtataatttt gcacatcaac tctcaaactt
     1381 accgactgac ctcgaccgat gagcctaaaa acaagtgaag ctggcgttaa gaaatagtcc
     1441 tgtttgagcg acaaaggagc gagttgggac tgttttagca agctgagcgt cggtttttag
     1501 agcgaggtgg gcggagtctt gagctttttt gattacttta ttgtgtcaag tggcacaccc
     1561 cgactttagt tcggggaaaa aaagtaatta gggtttgggg aaaagcccca tgtataaaat
     1621 aaattcttat acatgttgtt agcagaaaaa aagcgagaca aattaggatt taatacatat
     1681 agatatcaga gctacgttcc tctattatga cgaaaatcat tattttctac gaagaaatca
     1741 gaactacgtt cctttagcgt tagcaatttg tgatttaaac tgtaatgata ttgaactgtc
     1801 tatttctgaa ttacgtttat aaaaaaattg gactgttatt gctaacaatc caattctcac
     1861 aacctactat taaaaatcaa acttacattt cgttcaagtg ggtaaacttg taaacaaagg
     1921 gataaatttt acatgctaca caaaaattaa aggcagcttc taaaaaagca caaataccaa
     1981 atataaccac caaaacagta gcaacggaat ctacaccgaa caatgaaaat attgctatag
     2041 acaagctaaa taccaagcct atacgggctg caaatatctt tggaccagca tttgttttct
     2101 tgcctgtagt cgaaaatcta gctaatactg cttttgacaa ttttgccaaa atactgtaat
     2161 cgggtttttc aatggctcga gcaaaaaaat cagctaccaa aaacaggata ataaaataat
     2221 gctgtgtaag agcaaacaac acgagtaata gtacggtgat agcaccgttg atgcgtggaa
     2281 tacgctcgtc aatttgttgc gacgatatag gacaaatggc ttgtagtttc ataatggtat
     2341 atattagtga tttatataca gcaaagatac agcaaagata cagccacttc cactacccca
     2401 caatcccaca aagcgagtat tttttatacc atagttgtgg tatggaaaaa gatgaatgaa
     2461 caattttagg aaggttacac tactttttga aagaagtttc taaaactcca actgaaccga
     2521 taaactttgc aaggaaggtt tgatagaagc ccttttaatc cacttgtacg aatggaaatc
     2581 aattgctaaa gctgctatac tcaaaccagc tgacacgtaa agcaattctt tgtcaaatga
     2641 tcctgtgtaa tacaatatgc ttgttgccaa actcaatcca taacaaattt gtgcacaacg
     2701 cttttgggta taaaacctat taaggttgtg ttgtatccaa gcatcattat aggctgactc
     2761 tgataatttt ttgttctttt cataaaagct cccttcgatg atatacgcct tatcatacgc
     2821 gtctgttgca tctcttagtt gttttggttg aaaaccaatg gtctttacca atgaggcatc
     2881 aatagtacta atgtttgaat ctttattccg tttaaacgtc aaatagccat cttcaatatc
     2941 aagtactcta taaactgtat atatttctcc gcttt
//
LOCUS       DAOSMY010000200         2963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33403_length_2963_cov_3.378955, whole genome shotgun sequence.
ACCESSION   DAOSMY010000200 DAOSMY010000000
VERSION     DAOSMY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33403_length_2963_cov_3.378955"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1022
                     /locus_tag="PLD76_05090"
     CDS             273..1022
                     /locus_tag="PLD76_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOU27410.1"
                     /translation="MGFLGSFLGGALGFTVGGPIGAIIGVFLGSAISSNYGEKKKKNT
                     SQNDFKLSLLILIATVLKADGKVTKSELQETKAFLRANFGEEEALEALQMLKNILEQD
                     YDYKSICLQINQGLNYSSKLQLLQILFKISSADTIESSELRLLQTMATLMGISMGDFQ
                     SIQAIYSQHQHFHGQVKDTRKELDLAYQILEIDKNASDEEIKKAYRRMAMKYHPDKVS
                     TLGDEFKEIATEKFKAVKNAYELIKKEKGFA"
     gene            1050..>2963
                     /gene="rnr"
                     /locus_tag="PLD76_05095"
     CDS             1050..>2963
                     /gene="rnr"
                     /locus_tag="PLD76_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOU27411.1"
                     /translation="MKKKDIAINMMEVFKESPYKLFNYKQLASAINVKSQTDKRNVQQ
                     VLNELVALGSIEEMDRGKYRFSSKSKFVIGIIDKNSNGKTHLIPEDGGEKIFIADRNV
                     NRAMKNDTVKVLLYAARKNRQPEGEVVEIIKRSRETFVGVLSIKNNVAFVIIDNRILN
                     ADIYIPMDKLNGAKDGQKVLVKMTDWTPNAKSPFGEISDVLGEVGENNTEMHAILAEF
                     GLPYSYPEELERMAKNIDVGITPEEVAKRIDMRSITTFTIDPRDAKDFDDALSHRILD
                     NGNIEVGVHIADVTHYVKPHTPIDKEGFARAASVYLVDRTIPMLPEKLSNEVCSLRAN
                     EDRLCYSVIFELNDSADIVRYKIAKTVICSDKRFTYEEAQEIIEKKQGEFVSELLALD
                     TLAKKLRQKRFDLGAIAFDRYEVRFEIDEKGKPLSVYFKEQKDANKLIEEFMLLANKT
                     VAEHIGNVPKGKKAKTFVYRIHDEPNQEKLANFSLFIKRFGYKLKTTGKKKDIANSLN
                     HLLDEVTGKKEQNLIETLAVRSMAKAIYSTKNIGHYGLAFDHYSHFTSPIRRYPDMMV
                     HRLLTDYLEGGKSAPADKYEEYCEHCSDREQLAASAERASIKYKQVEFMKDKVGQVFD
                     GVISGITEWGIYVE"
BASE COUNT         1051 a          463 c          588 g          861 t
ORIGIN      
        1 aaatgcaatt agaaaaaatc ctatccaaat gtaattcaat accatattaa gaattattta
       61 acccacaaag ttacaaaaat atcgcatgct ttcgtatttt ttagcaattt tttcaaatct
      121 tctcctttcc tgctttttta tatgtaaaat ttaactgaat agtatttctc tttttgcgga
      181 taaacataaa acttccatgt ttttaattaa ttttaaatca taagttgaca aaaaatcaat
      241 acctttgtaa attaacaaaa atagagcgat aaatgggatt tttgggtagt tttttaggtg
      301 gagcattggg gtttacggtt ggtggaccta tcggtgccat tattggtgta tttcttggct
      361 cggctattag ttctaattat ggtgaaaaaa agaaaaaaaa cacctctcaa aatgatttta
      421 aattatctct tcttattttg atagccactg tattgaaggc tgacggaaag gtaacaaaat
      481 cagaattaca agaaacaaaa gcttttttaa gagcaaattt tggtgaagaa gaagcactag
      541 aagctctgca aatgttgaaa aatattttag aacaagatta tgattataaa tcaatttgtt
      601 tgcaaataaa tcaagggtta aattattcat ccaaattgca attgcttcaa attttattta
      661 aaatatcttc tgctgataca attgaatcgt ccgaattacg ccttttacaa acaatggcta
      721 cactaatggg tatttcaatg ggcgattttc aatctattca agcgatatac tctcagcatc
      781 aacatttcca tggacaagta aaagacacac gaaaagagct tgatttagcg tatcaaatct
      841 tagaaataga taaaaatgct tcggacgaag aaataaaaaa agcctatcgt cgcatggcta
      901 tgaagtatca tcccgataag gtaagtactt tgggcgatga gtttaaagaa attgcaaccg
      961 aaaagtttaa agccgtaaaa aatgcttacg aactgataaa aaaagaaaaa ggttttgctt
     1021 gataaaggca aacgataata acaatacgta tgaagaaaaa agacatagca ataaatatga
     1081 tggaggtttt taaagagtct ccatataagt tatttaatta caaacagtta gcttctgcta
     1141 tcaatgttaa atcccaaacg gacaaacgga atgtgcaaca agtacttaac gaattagtag
     1201 ctcttggttc gatagaagag atggataggg ggaagtaccg ttttagttct aaaagtaagt
     1261 ttgtaatagg aataattgat aaaaatagta acggaaaaac acatttaatt ccagaagacg
     1321 gcggagaaaa aatatttatt gctgatagaa atgtaaaccg tgcaatgaaa aatgatacgg
     1381 tcaaagtatt gctttatgcc gctcgtaaaa accgtcaacc agaaggcgaa gttgttgaaa
     1441 taattaaacg gtctagagaa acttttgttg gcgtgttgtc tatcaaaaat aatgttgcct
     1501 ttgtgattat tgacaatcgg atattaaatg cggatattta tataccaatg gacaagctca
     1561 atggggctaa agatggtcaa aaagtattgg tgaaaatgac cgattggacg cctaatgcca
     1621 aaagtccgtt tggtgaaatt agcgatgtgc ttggtgaagt aggtgagaat aataccgaaa
     1681 tgcacgccat attagcggaa tttgggttac cctattctta cccagaagaa ttagagcgta
     1741 tggcaaaaaa tattgacgta ggcattacgc ccgaagaagt agccaaacgc attgatatgc
     1801 gttctattac cacctttacc atcgacccgc gcgatgccaa agattttgac gacgctcttt
     1861 cgcatcgcat tttagacaat ggaaatatag aagtgggcgt acacattgcc gacgtaactc
     1921 attatgttaa accacatacg cctattgaca aggaggggtt tgctcgagct gcttctgtct
     1981 atttagtaga tagaaccata cctatgttgc ccgaaaaact gtcgaacgaa gtatgttcgt
     2041 tacgtgccaa cgaagacagg ctgtgttatt cggtgatatt tgagttgaat gatagtgcag
     2101 atattgtgcg atacaagatt gcaaaaacgg ttatttgttc cgacaaacgt tttacatacg
     2161 aagaggctca agaaataata gaaaagaaac aaggggagtt tgtgtcagaa ttgttggctt
     2221 tggatacgct tgctaaaaaa cttcgacaaa aacgttttga tcttggtgcc attgcttttg
     2281 atagatacga ggtgaggttc gaaatagatg aaaaaggaaa accattaagt gtgtatttta
     2341 aagagcaaaa agatgccaat aaattgatag aagaatttat gttgcttgcc aacaaaacgg
     2401 tagccgagca tatcggaaat gtacccaaag gaaaaaaagc taaaacattt gtgtatcgta
     2461 ttcacgacga gccaaatcaa gaaaaactgg caaatttctc ccttttcatc aagcgttttg
     2521 gttataaact caaaacaacg ggtaagaaaa aagacattgc caattcgctc aatcacttat
     2581 tagatgaagt aacaggaaaa aaagagcaaa atttaatcga aacattggct gtgcgttcta
     2641 tggcaaaagc gatttattcg actaaaaaca taggtcatta cggtttagct tttgaccatt
     2701 attctcattt cacctcgccc atccgtcgtt atcccgatat gatggtgcac cgtttgctaa
     2761 cagactattt ggaaggtgga aaatcagctc ctgctgataa atacgaagag tattgcgaac
     2821 attgttcaga tcgcgaacaa cttgctgctt cggcagaaag ggcttctatc aagtacaagc
     2881 aagtagagtt tatgaaagat aaggtagggc aggtatttga tggggttatt tcaggtatta
     2941 ccgaatgggg catttatgtg gaa
//
LOCUS       DAOSMY010000201         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33488_length_2957_cov_4.319779, whole genome shotgun sequence.
ACCESSION   DAOSMY010000201 DAOSMY010000000
VERSION     DAOSMY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33488_length_2957_cov_4.319779"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(595..1710)
                     /gene="dprA"
                     /locus_tag="PLD76_05100"
     CDS             complement(595..1710)
                     /gene="dprA"
                     /locus_tag="PLD76_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU27412.1"
                     /translation="MDNKLLYQIAITQIKGLGSINSKNLLAYVGDIEEIFHQKSHILQ
                     KIPGIGILLSKSILEQKDAALEKATKEIQFITKHGITPLFYTDKNYPERLKECPDAPI
                     LLYYKGNTNLNTQKTLAIVGTRNATEYGKTMCTQLIKELANNHPELCIISGLAYGIDI
                     CAHKEALHMHLPTVAVLGHGLDRIYPAQHATIAKKILTSGGLLTEFPSETNADKPNFV
                     RRNRIIAGTTDATLIVESAARGGAIITTEIANSYNRDVFAVPGKCTDEWSVGCNNLIK
                     QHKAALVNSASDIEYIMGWTKEQTSQKIEQQQSLFIDLSDTEQEIFTIIKQNKVVHID
                     VLAIKISKSIKDLSPILLSMEFKGVIKCLPGNIYSVV"
     gene            complement(1703..2074)
                     /locus_tag="PLD76_05105"
     CDS             complement(1703..2074)
                     /locus_tag="PLD76_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU27413.1"
                     /translation="MLQFVRSYWKTIVTAIFIAYLSLFKPNSTIQLPPIPFVDKWIHI
                     VLYTTLTAVALLDTKKRKNNTSSFLLVLLISILYGGLMEILQQFFPPRTSSWYDFLAN
                     ILGCLLAFGSITWFVKKKRNG"
     gene            complement(2085..>2957)
                     /gene="hemW"
                     /locus_tag="PLD76_05110"
     CDS             complement(2085..>2957)
                     /gene="hemW"
                     /locus_tag="PLD76_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU27414.1"
                     /translation="LVEITVEVNPDDVNDLLLDTLKSIGCNRLSMGIQSFFDHELTVL
                     GRRHTAQQAIEAVNLAQQHGFSNISIDLMYALPNQTIQTWRETLKLAISMNVQHISAY
                     HLTYEPGTLFYKQKTKKIITEKDEEESIQLYEILIQSLTEAGYNQYEISNFSLPDYES
                     KHNSSYWKNTPYIGLGASAHSYNGASRQWNIASVITYCNQIEENKSFFEIEFLTEKEK
                     YNDFIITSLRTSKGVDLQKLTTLFGEKSRSYFIQQCQPYVHTNMLCEKDNFVRLTQKG
                     IFVSDSILETLIDV"
BASE COUNT          916 a          452 c          493 g         1096 t
ORIGIN      
        1 attttcgtta ttgttattat caacagtatt ctttattctt ttatttattt aaaaaagtta
       61 taaaaaagaa tgataagaat aatagggtgt tgataaggtt tataaataaa acactaaaaa
      121 tttgcattat taaaatacgg tgtaatatac agagtatttc aacagataat taacagtata
      181 atatgctgat aaaaaatgta ttgataaggt tattaacata ttgtttacaa ctacaaatgt
      241 agtaaacttt tcgttttttt atggttaata accgctcttt ttattgttca ttactttttt
      301 ttaaaataca taactatatt cttgtttttt taaaattttg tatagtaaac atcataattt
      361 acaactacaa atttttttat cattttttac tctctatttt attaacagct tttaaacaat
      421 aactgtgaat atactaacaa tgcaaacgct tgcgtaaata attatgcaaa ttatgcttaa
      481 atttgataca atatcctata aatcagtata cttttgtagt gtgattttca aagtatttga
      541 tttaaggtta ataaaaaggg attaatacta gcaaggtatt agtccctttt gtgtttatac
      601 taccgagtaa atattacctg gtaaacattt tattacacct ttaaattcca tagaaagcaa
      661 tatgggcgat aaatctttta tgcttttgct tatttttatc gccaatacat ctatatgaac
      721 gactttattt tgtttgataa ttgtaaaaat ttcttgttct gtatcggata aatctataaa
      781 caaagattgt tgttgttcta ttttttggct tgtttgttct tttgtccaac ccataatata
      841 ttcaatatca gaagcactat tcactaatgc tgctttgtgc tgttttatta ggttattgca
      901 acctacagac cattcatcag tacatttacc aggcacagca aacacatctc tgttatatga
      961 attagctatt tcggtagtaa taatagcacc accacgcgca gcactttcaa caataagtgt
     1021 agcgtctgtt gtacctgcga taatgcgatt tcgacgtaca aaattgggct tatctgcatt
     1081 tgtttcgctc ggaaattctg taagtaaacc gcctgatgta agtatctttt tggctattgt
     1141 agcatgctga gcagggtaaa ttctgtctaa accatgaccc aatacagcta cagttggtaa
     1201 gtgcatgtgt aaggcttctt tgtgagcaca aatatctatg ccataagcta atccactaat
     1261 gatgcacaat tcaggatgat tgtttgctaa ctctttaata agttgagtac acattgtttt
     1321 accatattca gttgcattgc gtgtgcctac tatggctaag gttttttgtg tatttaaatt
     1381 tgtatttcct ttataataca acagaattgg agcatctgga cattctttta atcgttctgg
     1441 ataattctta tcggtataga aaagaggtgt aatgccatgt ttggtaataa attgaatttc
     1501 tttggtggct ttttcgagtg cagcatcttt ttgttcaaga atgcttttgc ttaacagaat
     1561 cccaataccc ggtatttttt gtaaaatatg cgatttttga tgaaatattt cttcaatatc
     1621 acccacatag gcgagtaaat ttttactatt tatactgcct aatcctttaa tttgagtaat
     1681 ggctatttgg tatagaagtt tgttatccat ttcttttttt cttaacaaac catgtaatac
     1741 taccaaaagc aagtaaacag ccaagtatat ttgccaaaaa atcgtaccaa ctgctggtgc
     1801 gaggagggaa aaactgttgc aatatttcca tcaaacctcc atatagaatg ctaattaaca
     1861 atacaagaag aaatgatgac gtattatttt ttcttttttt tgtgtcaagt agggctactg
     1921 ctgttagcgt agtatacaga acaatgtgta tccatttatc tacaaaagga ataggaggta
     1981 gttgaatggt gctatttggt ttgaatagcg acaaatacgc aatgaagatt gctgttacaa
     2041 tggttttcca atatgaacgt acaaattgta gcattgcaga gttgttacac atcaattaat
     2101 gtttctaaaa tagaatcgga tacaaaaata cctttttgag taagacgcac aaaattatct
     2161 ttttcacaaa gcatgttggt atgtacgtat ggttggcatt gttggataaa ataacttctt
     2221 gatttttctc caaatagcgt agtaagtttt tgcaaatcaa cccctttgct agtacgaagg
     2281 gaagtgatga taaaatcgtt gtatttttct ttttctgtta agaattcaat ttcgaaaaat
     2341 gatttatttt cttcaatctg gttgcaatag gtaattacag atgcgatgtt ccattgccga
     2401 gatgctccgt tgtaggaatg agccgaagca cccaatccaa tatagggtgt atttttccag
     2461 tacgatgaat tgtgttttga ttcgtagtca gggagtgaaa aattcgaaat ttcgtattga
     2521 ttataaccag cttcggttaa cgactgtata aggatttcat acaattgaat actttcttct
     2581 tcgtcttttt cggtgattat ctttttcgtt ttttgcttgt aaaagagtgt gccaggttcg
     2641 taagttaaat gatacgccga aatatgttgc acattcatcg aaatggctag ttttaatgtt
     2701 tccctccacg tttgtattgt ttggttgggt agggcataca taaggtcgat actgatattc
     2761 gaaaaaccat gttgttgagc caaattaact gcctcgatgg cttgttgggc ggtatgcctg
     2821 cgtcctaaaa cagtaagttc gtggtcgaaa aacgattgaa tgcccatact caatctatta
     2881 caacctatgg atttgagtgt atctaacaat aaatcattca cgtcatcagg attcacttct
     2941 actgttattt caactaa
//
LOCUS       DAOSMY010000202         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33550_length_2952_cov_4.788402, whole genome shotgun sequence.
ACCESSION   DAOSMY010000202 DAOSMY010000000
VERSION     DAOSMY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33550_length_2952_cov_4.788402"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2618)
                     /locus_tag="PLD76_05115"
     CDS             complement(<1..2618)
                     /locus_tag="PLD76_05115"
                     /inference="COORDINATES: protein motif:HMM:NF039454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6443 domain-containing protein"
                     /protein_id="HOU27415.1"
                     /translation="MKKILSLFVLLPLMVFAQTQTENYIKNTTYKTPTTTRISTPTIS
                     QASQNITYFDGLGRPIQQIAHQQSGTGKDIVTPIEYDGFGRQPKDYLPYVPTASASLD
                     YKTSALTDVLNFAPYTGQNPFSEKQLEASPLNRVFKQAAPGTDWALNTGHEIKLDYQT
                     NTSSDAVKLFSVTANWDAAKGLYDIPTSLTPADYLDFQLYKSITFDENTSATPTESNG
                     STVEFKNKEGQVILKRTYESGVKHDTYYVYDQYGNLTFVIPPLANATTTISNTILNDL
                     CYQYKYDSRNRLVEKKLPGKQWEFIVYDKLDRVVATGPALSPFSDLTTAGWLITKYDG
                     FSRVVYTGWENSIAESSTRTTKQSAQNGLTTSLNEAKTAPGAIDGIATFYTNSVAPTA
                     FKLLTVNYYDDYNFNNAPTFPAYSYDGVPAYYNNTTMKPKGMPTGSWVRVVTLSSSLA
                     AETSYIIYNDKARPVTNYTTNYLGGFTKTELKYDFIGNTTSNHTNHKRSSTDPQILVN
                     EGFTYTPQGRLLTHTHQINNGVVQLLASNTYDELGQLISKNVGNTTAAPLQKVDYVYN
                     IRGWMTEINKVAALQQGSDPKDLFGFKINYNTIDGDASATKKLYNGNIAETSWSTSTV
                     VRTYGYKYDNLNRLTFATYQKSGITTNAYNENLTYDKNGNIKHLNRFGLLDPQTPAPQ
                     IDDLTYEYKSSTSNQLTKVTDSPSGNDNEGFIDGNKSGDDYTYDANGNLITDLNKNIT
                     QIVYNHLNLPTKITFGASGNIVYIYNAIGQKLEKIATQGTVTNTNYLGGFQYQSVNGA
                     TMALQFFPTAEGYVKNTAGVYSYVFNYTDHLSNVRLSYGLNDNVLTIFEENNYYPFGL
                     KHKGYNEYTPTNNKYK"
BASE COUNT          816 a          478 c          613 g         1045 t
ORIGIN      
        1 tacttatact tattattggt cggcgtatac tcattatacc ctttatgttt tagtccaaaa
       61 gggtaataat tattttcttc aaaaatggta agaacgttgt catttagtcc gtaactcaat
      121 ctaacattgc ttaaatggtc ggtgtaatta aaaacataac tgtaaactcc agcggtattt
      181 ttcacatagc cttcggctgt tgggaaaaat tgcaaagcca ttgtggctcc attaacactt
      241 tggtactgaa aaccgccaag ataattggtg tttgttacag tgccttgggt agcaattttc
      301 tctagttttt gtccaattgc gttgtaaata taaacaatgt ttccactagc tccaaaagtt
      361 attttggtgg gtaggttgag gtgattgtaa acaatttggg tgatgttttt gtttaagtcg
      421 gttatcaaat taccgttggc atcataggtg tagtcatctc ctgatttgtt accatcaata
      481 aatccttcat tatcattacc actagggctg tcggttactt tagtgagttg gttggaagta
      541 gatgatttat actcatacgt taaatcgtct atttgtggag caggcgtttg agggtctaga
      601 agtccaaaac gattaaggtg tttgatattt ccatttttat catacgttag gttttcatta
      661 taggcatttg tggtaatccc tgatttttga taggtggcaa atgttaagcg gttgagatta
      721 tcgtatttgt acccataggt tcggactact gtagaagtac tccaagaagt ttcagcgatg
      781 tttccgttgt ataacttctt agtcgcactc gcatcaccat caatagtatt gtagtttatt
      841 ttgaacccaa acaaatcctt agggtctgag ccttgttgta aggcagctac cttgtttatt
      901 tcggtcatcc aaccacgtat attataaaca taatctactt tttgcagcgg tgcagcggta
      961 gtgttcccca catttttgga aataagctgt ccgagttcgt cgtaagtatt tgatgccaag
     1021 agttgtacca ctccattgtt tatttgatgg gtatgagtaa gtaggcggcc ttgcggggta
     1081 taagtaaaac cttcgtttac caagatttga ggatcggtac tggatcgttt atggtttgta
     1141 tgattacttg ttgtgttacc aataaaatcg tatttaagtt cagttttggt aaagcccccc
     1201 aaatagttgg ttgtgtagtt tgttacaggt ctggctttgt cgttgtatat aatatatgag
     1261 gtttcagctg ctaaagagct gctcaaagtt actactctca cccaagaacc cgttggcatt
     1321 cctttgggtt tcattgtcgt attattataa tatgcaggta caccatcata actataagca
     1381 ggaaatgtag gcgcattatt aaaattataa tcgtcataat aatttacagt tagcaacttg
     1441 aatgctgtag gggcaacact attggtatag aaagtagcga tgccatctat ggctccgggt
     1501 gctgttttag cttcatttaa tgaagttgta agtccgttct gtgccgattg cttggttgtt
     1561 cttgtactag attcagcaat tgaattttcc caaccagtat aaaccactct gctaaagcca
     1621 tcatacttag taattaacca gcctgctgtt gttaaatcgc taaaaggaga aagagcagga
     1681 ccagtagcca ccacacgatc gagtttgtca tacacgataa attcccattg cttgccaggc
     1741 agcttttttt ctaccagacg attgcgagaa tcgtatttgt attggtagca taaatcattg
     1801 agtattgtat ttgaaatggt ggtggtagca ttcgctaaag gaggtattac aaaagtcaaa
     1861 ttaccgtatt ggtcatacac ataataggta tcgtgtttta cacccgattc ataggttctt
     1921 ttcaaaatca cttgtccttc tttatttttg aactctacgg tcgaaccatt ggattcagtt
     1981 ggagttgctg atgtgttttc atcgaatgtt atgcttttat agagttgaaa gtcgagataa
     2041 tcagcaggag tcaaagaagt aggaatgtca tacagccctt tggcagcatc ccaattggca
     2101 gtaacagaaa atagttttac ggcatctgaa gaggtattgg tttgataatc tagttttatt
     2161 tcgtgacccg tattcaacgc ccaatccgtt cctggagcgg cttgcttgaa tacacggttg
     2221 agaggggagg cttctaattg tttttcacta aagggatttt gtcctgtgta tggtgcaaag
     2281 tttaaaacgt ctgttaacgc actagtttta taatcgagag aagcagatgc ggttggtaca
     2341 taaggcaaat agtctttggg ttgacgccca aaaccatcat attcaatggg cgtaacaatg
     2401 tctttaccag ttcccgattg ctggtgggca atttgctgga tgggacgacc tagaccgtca
     2461 aaataggtaa tattttgact ggcttggctg atagtaggtg ttgaaatacg ggtggttgtt
     2521 ggtgttttgt aggttgtgtt tttgatgtaa ttctcggttt gggtttgagc gaaaaccatt
     2581 agtggtaaca aaacaaaaag ggataatatt tttttcataa ctctttcgga ttagttttta
     2641 taatggtatt cgttctcgga taagatatta ccgtttttgt cttttacatt tttcaacctt
     2701 atgtgattta caatgatctc accataaaac atgtagcaat ttcagaatag ttttttgaag
     2761 tattttacgg taaaaccgta aaaattacta aagtcatttt actgttttat ttgatagagt
     2821 taaatagatt ttgcaacata taaaaaatat ttattcttat tttatttttt gcagttttaa
     2881 aagctggtaa taaaatcgaa aatattgtat ttaaaaataa tgactttttg aaggtatgta
     2941 tttatctttc tg
//
LOCUS       DAOSMY010000203         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33585_length_2950_cov_3.017271, whole genome shotgun sequence.
ACCESSION   DAOSMY010000203 DAOSMY010000000
VERSION     DAOSMY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33585_length_2950_cov_3.017271"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1786
                     /locus_tag="PLD76_05120"
     CDS             107..1786
                     /locus_tag="PLD76_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOU27416.1"
                     /translation="MDTAENKKSLNFIESIVEKDLKEGKNDGRVQTRFPPEPNGYLHI
                     GHAKAICMDFGIAERFGGICNLRFDDTNPITEDVEYVDAIKHDIQWLGFQWNSIYYAS
                     DYFQQLFELAVRLIKEGKAYVDEQSSEEIAKQKGTPTTPGIESPYRNRPIEESLHLFD
                     KMNAGEIAEGAMVLRAKIDMSSSNMHFRDPIMYRIINTRHHHRTGDKWKVYPMYDYAH
                     GQSDFFEGVTHSICTLEFEVHRPLYDWFIDQLATTNYRPQQYEFNRLNITYTVMSKRK
                     MLQLVKEKLVAGWDDPRMPTICGLRRRGYTPSAIRSFINMIGYTKYEGTIDASLMEHA
                     IREDLKPKADRVCAILDPIKLIITNYPANQLELLSTQNNPDNEQAGERTIQFGKELYI
                     ERSDYMENAPKDYFRLTEGKEVRLKSAYIVTCTGSKKDENGRVVEVYCTYDAETKSGT
                     EGSKRKVKSTINWVEIGSSIDAEIRLYDRLFAIEDPSKSDQDFRDLLNPDSLHILKGA
                     KVETYLQNAKPMDSFQFLKHGYFTVDLDSKPNHLIMNRTVSLKDNTPKEKK"
     gene            1876..2454
                     /locus_tag="PLD76_05125"
     CDS             1876..2454
                     /locus_tag="PLD76_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOU27417.1"
                     /translation="MNKKLILPISIISIVVILVIWAFSTYNSIVTLDEAVPAQWAQVE
                     NAYKRRADLIPNLVSTVKGYAAHEQGTFEAVVEARAKATQITVDPSNLTPEKLKEYQA
                     AQGQVGSALGKLLMITENYPELKANQNFLELQAQLEGTENRISTERHRFNESTRMFNS
                     KIRRFPGNLFGFDTRPYFEATEAEQAVPTVSF"
     gene            2457..2903
                     /locus_tag="PLD76_05130"
     CDS             2457..2903
                     /locus_tag="PLD76_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOU27418.1"
                     /translation="MNPKTFFSKADQLRIENAIVEAEKQTSGEIRLHIQSLTKGDVMD
                     AAVGVFYRLEMNKTEQRNGVLFYLSINDHQFAVIGDSGINKVVPSNFWDSIYEGIISY
                     FKQNLYADGLVWAISQAGIQLSSFFPLQKDDTNELSNEISFETKNE"
BASE COUNT         1081 a          522 c          551 g          796 t
ORIGIN      
        1 cctcagaaag aggtattatt aaaggatatt ttgataaata aaacaaacga aggaagctgt
       61 caataaaaag gcagcttttt tcaaaaaata taacttgatt gttactatgg acacagcaga
      121 aaataaaaag tcattaaatt tcattgaaag tattgttgaa aaagacctca aagaaggtaa
      181 aaatgatgga cgtgtgcaaa cacgttttcc accagaaccc aacggatacc ttcatatagg
      241 tcatgccaaa gctatttgca tggattttgg tattgctgaa cgatttgggg gtatatgcaa
      301 tttacgtttt gacgatacaa atcctataac agaagatgta gaatatgtag atgcaataaa
      361 acatgatatt caatggttag gatttcagtg gaattcaatt tactatgctt ctgattattt
      421 tcaacaatta tttgaattag ctgtaaggtt aataaaagaa ggaaaagcct acgtagacga
      481 acaatcatct gaagaaatag ccaaacaaaa aggaacccct actacacctg gtatcgaaag
      541 tccgtatcga aatagaccca ttgaagaaag tttacatttg tttgataaaa tgaatgctgg
      601 agaaattgct gaaggagcaa tggtattgcg tgccaagata gatatgtcat cgtcaaacat
      661 gcactttcgc gacccaatta tgtaccgtat tataaacaca aggcatcatc atcgtacagg
      721 cgacaaatgg aaggtatatc cgatgtacga ttacgctcat ggtcagtccg atttttttga
      781 gggagtaacg cattctatct gcaccttgga gtttgaggta caccgccctt tatacgattg
      841 gtttatcgac caattagcaa caaccaatta ccgtcctcaa caatatgaat ttaatcgcct
      901 aaatattaca tataccgtaa tgagtaagcg aaaaatgtta caattagtaa aagaaaaact
      961 agtagcagga tgggatgacc cacgtatgcc aaccatttgt ggcttacgca gaagagggta
     1021 tacaccaagt gctatacgca gctttatcaa tatgattggc tataccaaat acgaaggcac
     1081 aattgacgca tcattgatgg aacatgctat acgtgaagat ttaaaaccta aagcagatag
     1141 agtatgtgcc attttagacc ctattaaact cattattaca aactatccag caaaccaact
     1201 ggaattattg tcaacgcaaa acaatccaga caatgagcaa gcaggtgaac gcaccataca
     1261 atttggcaaa gaattgtata ttgaacgttc tgattacatg gaaaatgcac ctaaagatta
     1321 tttccgcttg accgaaggaa aagaagttcg actaaaaagt gcctatattg taacatgtac
     1381 aggctctaaa aaagatgaaa atggacgtgt tgtagaagta tattgtacgt atgatgctga
     1441 aaccaaaagt ggtacagagg gaagtaaacg aaaagtaaaa agcaccataa actgggttga
     1501 aattggcagt agcattgatg ctgaaatacg cttgtacgac cgattatttg ccatcgaaga
     1561 tccatctaaa tcagaccaag attttcgcga tttactaaac cccgactcct tacacatact
     1621 aaaaggagca aaagtagaaa catacttaca aaatgcaaaa cccatggata gttttcaatt
     1681 tttgaaacat ggctatttta cagtcgattt agattcaaag cctaatcatc tgataatgaa
     1741 cagaactgtt tctttaaaag acaatacacc taaagaaaaa aaataataac gaataatgca
     1801 cctagtgata tgttcggcat tttgaaaaat aagtaatttt aagtacattt gtatctgaat
     1861 aaaatattat caactatgaa caagaaactc attcttccaa tttccattat cagtattgta
     1921 gtaatactcg tgatatgggc atttagcacg tataatagta ttgtaacact cgatgaagct
     1981 gttcctgcac aatgggcaca ggtagaaaat gcgtacaaac gtcgtgctga tttaataccc
     2041 aacctagtaa gcaccgtgaa aggatacgct gcacacgaac aaggcacatt tgaagctgtg
     2101 gtagaagctc gtgctaaagc aacgcaaatt acagttgacc cgagcaactt aactcctgag
     2161 aaactaaaag aataccaagc agctcaaggt caagtaggct ctgcattagg taaactattg
     2221 atgattaccg aaaattatcc agaactaaaa gccaatcaaa actttttaga attacaggca
     2281 caacttgaag gaactgaaaa tagaattagt acagaacgtc atcgtttcaa cgaatcgact
     2341 cgtatgttca atagcaaaat acgtcgtttc ccaggaaact tgtttggatt tgataccaga
     2401 ccctattttg aagctactga agctgaacag gctgtaccaa ctgttagttt ttaagaatga
     2461 atccaaaaac ttttttttca aaagctgacc aactacgcat tgaaaatgcc atagtagaag
     2521 cagaaaaaca aacctcggga gaaatacgtt tgcacatcca atcactcacc aaaggggatg
     2581 taatggatgc tgcagtcggt gtcttttatc gtttggagat gaataaaaca gagcaacgca
     2641 atggtgtgtt attttatctt tctattaacg accatcaatt tgctgtaatt ggagatagcg
     2701 gtattaacaa agtagtgcca agcaattttt gggacagcat ttacgaagga attatttcct
     2761 attttaaaca aaacctgtat gcagatggat tggtatgggc aatatcgcag gcaggcattc
     2821 agctatctag ctttttccca cttcaaaagg atgataccaa cgaattgagc aacgaaattt
     2881 cttttgaaac aaaaaatgaa taacatgcac caaacagtaa aaactttggg actaatgcta
     2941 tgtatgctta
//
LOCUS       DAOSMY010000204         2927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_33907_length_2927_cov_3.502089, whole genome shotgun sequence.
ACCESSION   DAOSMY010000204 DAOSMY010000000
VERSION     DAOSMY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33907_length_2927_cov_3.502089"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..832
                     /locus_tag="PLD76_05135"
     CDS             185..832
                     /locus_tag="PLD76_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU27419.1"
                     /translation="MQHIIIADNQAISRFGIYQLLSKQAIQYQTTECNTKDQLVLMLR
                     QHTNAIIMLDYTLFDFLGIDDLLVLAERYPLAHWLLLSDELSPDFLQKALLSANNISV
                     VMKDATQEELLFAMQHVVRKERFVCNYVSNLLLSAKTIDANYAIQLTDTEKIILKEIA
                     LGYTTKEIAAKKFISFHTVNTHRKNIFRKLGINNLHEATKYAMRAGIIDMAEYCI"
     gene            1031..2215
                     /locus_tag="PLD76_05140"
     CDS             1031..2215
                     /locus_tag="PLD76_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27420.1"
                     /translation="MMHSKTYKWGLFIFLMLVLGIGIFVWFNTKHFYTNFEIKDELGG
                     NIFPSSILSVATTDTQVIMPSDSVYVGNPKSGIPISIRSGKAFSRVRIEIAETRFFAR
                     SVSEFVLEKENTEYLIYPDVIWNYDALRNNSQAEPISVVITAELNGKPLGQRVRTYSV
                     RSINECLLGYVSNGTKYHDTGIFFAAYVNEDHPMIDQILREALNTRIVTRFLGYQART
                     PEDVDKQVYALWNVLQRRNFQYSSVANTSLSSNVVFSQRIRTFDDALNSSQINCVDGS
                     VLFASLLRAINMEPILVRMPGHMFVGYYTNSKRSNMNFLETTMIGDVNLDDFFPDEQL
                     DSTMVGKSKNQMSRITFEKSKEYANKKYEEVKENVKAEKPNYMFLVISKDVRRKIQPI
                     GK"
     gene            complement(2574..>2927)
                     /locus_tag="PLD76_05145"
     CDS             complement(2574..>2927)
                     /locus_tag="PLD76_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27421.1"
                     /translation="FYNQFRQMKKLFFLTTIAISTVFFAKADPAKKVNLTYQNGKLKI
                     EAIHKVKDPQKHYIDQIVINIDGKDVKTVTLKFQNSKDAELLEIPIDLKKGTKVKATT
                     RCNEFGIKKGEITIE"
BASE COUNT          996 a          540 c          486 g          905 t
ORIGIN      
        1 agggtataat ctcagcctga gaaaaactca agcaccccat acatatcgat gcaaaagtac
       61 acattatttt tagaataatt gaaatatgaa agaaaaatta tgttttataa catatcagaa
      121 ggtttgttaa aagtataatc aataaaaaaa aagtgtatct ttgttataag aaaacatagc
      181 aactatgcaa cacatcatca tagccgacaa ccaagccata agccgttttg gaatatacca
      241 attgctaagc aagcaagcta tacagtacca aacaaccgaa tgcaatacca aagaccaatt
      301 ggtattgatg ttacgccaac ataccaacgc aataatcatg ttggattata ccttgtttga
      361 ctttttaggt attgacgatt tattggtgtt agcagaacga tatccgctag cacattggtt
      421 gctattatca gacgaattaa gtcctgattt tttacaaaaa gccttgttaa gtgctaacaa
      481 tataagcgtt gtgatgaaag acgctacgca agaagaacta ctatttgcta tgcaacatgt
      541 agtgcgaaaa gagcgttttg tgtgcaatta tgtaagtaat ttattgcttt ctgccaaaac
      601 gatagatgct aattacgcca tacaattaac cgataccgaa aaaataatac tgaaagaaat
      661 agctttgggt tacaccacca aagaaatagc tgcaaaaaag tttatcagtt ttcataccgt
      721 aaatacacac cgaaaaaata ttttccgcaa actaggaatt aacaacctac acgaagccac
      781 caaatatgct atgcgagcag gcattattga tatggcggaa tattgtatct aaaaaggcat
      841 aacaaacaca atctacatcc tgtatcactg ctgataaaac aatgaaaatt gtacacttac
      901 aaaaaaaact tatttttaga atctgaattt taaacatttt aaaagattaa ttactaaaaa
      961 ttaatccatc tatttgtgtt aaaatagact atctttgtac attcgtatat atcgtttgaa
     1021 cattctatct atgatgcata gtaaaactta caagtggggt ctatttattt ttctgatgct
     1081 tgtgttagga attggaattt ttgtttggtt caacacaaaa cacttttaca caaattttga
     1141 aataaaagat gaactaggcg gaaatatctt tccttctagc attttatctg tggcaactac
     1201 cgatacacaa gtaattatgc ctagcgactc tgtctatgta gggaatccta aatcaggcat
     1261 tccaatttct atccgttctg gcaaggcttt cagtagagta cgtattgaaa ttgccgaaac
     1321 acgtttcttt gctcgttcgg tatctgagtt tgtactggaa aaagaaaaca ctgaatacct
     1381 catttaccca gatgtaattt ggaattatga tgccctacgc aacaacagcc aagcggaacc
     1441 catcagcgtg gtaattaccg ctgaactcaa cggaaaacca cttgggcaac gcgtacgcac
     1501 gtactctgtg cgaagcatca acgaatgctt gcttggatat gtaagcaatg gaacaaaata
     1561 ccacgataca ggaatctttt ttgctgctta cgtaaacgaa gatcacccca tgatagacca
     1621 aatactgcgt gaagctctta acacacgaat cgttactcgc tttttgggtt atcaagcacg
     1681 gactcccgaa gatgttgaca aacaggtata tgccctgtgg aatgtattac aaaggagaaa
     1741 tttccaatac agttctgtag ccaatacaag cctatcaagc aatgtggtgt tttcgcaacg
     1801 aatacgtacg tttgatgatg ccctaaattc atcacaaatt aattgtgtag atggtagcgt
     1861 actttttgcc tccttactgc gggctattaa tatggaaccc attttggtac gcatgcctgg
     1921 gcacatgttt gttgggtact ataccaactc caaacgctcc aacatgaatt ttctggaaac
     1981 aaccatgatt ggcgatgtaa acctcgacga ttttttcccc gacgaacaac tagattctac
     2041 catggtagga aaatcgaaaa accaaatgtc gcgtatcaca tttgaaaaat caaaagaata
     2101 cgccaacaaa aaatacgaag aggtaaaaga aaacgtaaaa gcagagaaac ccaactacat
     2161 gtttttggtt atctccaaag atgtgcgtag aaagatacaa cctataggaa agtaaacttc
     2221 ttgctccgta acccaataat gccttgttgt tttgtttaca tgttacttca cagagttaca
     2281 cagagtattt aataccccgt aggggctaca tcataataga aaatacatac cgatttgttt
     2341 gttagctcca gcggagcgga atagatagtt gtctccgtaa tcctaacatg ctttgttgta
     2401 ttgtttccat tatacacttt tttgtgtcaa cttagttcag gaaaagacac atcatcctgc
     2461 ctataaacat aggtgcgaat aagcgtttag gcattgtatc caaacaacca atctattggt
     2521 tttccaagca aaaaaatcga actactcaaa tagcaactag tgtctattgc tatttattca
     2581 attgttattt cacctttttt gataccaaat tcattgcaac gtgtagttgc ttttacttta
     2641 gttccttttt ttaaatctat tggtatctct aataattcag catcctttga attttgaaat
     2701 ttcagtgtaa cggtttttac atcttttcca tcaatattta taacaatttg atcaatgtaa
     2761 tgtttttgag gatctttcac cttgtgaata gcttcaattt taagttttcc attttgataa
     2821 gtcagattca ctttttttgc tggatcagct tttgcaaaaa agactgttga aatagcaatt
     2881 gttgttaaaa aaaataattt tttcatttgt ctaaattgat tataaaa
//
LOCUS       DAOSMY010000205         2918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_34053_length_2918_cov_3.020608, whole genome shotgun sequence.
ACCESSION   DAOSMY010000205 DAOSMY010000000
VERSION     DAOSMY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34053_length_2918_cov_3.020608"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLD76_05150"
     CDS             complement(<1..121)
                     /locus_tag="PLD76_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOU27422.1"
                     /translation="MIIGENIKQAALIIREGGLVAFPTETVYGLGANALNPMAV"
     gene            complement(126..602)
                     /locus_tag="PLD76_05155"
     CDS             complement(126..602)
                     /locus_tag="PLD76_05155"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOU27423.1"
                     /translation="MKVIILMGSKADLDWGTQIKTVLDRFEIPSVIRIASAHKVPLKC
                     YALIKEYEKENVIFITIAGMSNALSGFSDAQTYCPVIACPPYSDKFGGTDIFSSVRMP
                     SGVAPLTVLSPENAALAAAKIFGLSNPSLREKIAAFQEKKREELEEVDALLQKSQA"
     gene            complement(741..2633)
                     /locus_tag="PLD76_05160"
     CDS             complement(741..2633)
                     /locus_tag="PLD76_05160"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOU27424.1"
                     /translation="MTTKLFKRLQEKNVTLPDDVQILLNSCKGFTLFETVEDIADAAV
                     GGKDKLEFEVKYDVPGKGEYTEAIVHRVTNGISANYTEAYMRRRDPNTMAIGDNLPTD
                     KKRFKDKYGYEFSNLRKETFDWLKTQELAIFFYFAGNNGIGVPGIAIVPANAGFFAMG
                     LAMLQHIVPINEMEDSFAIESVLYVAPVFRHTHFEGKQVVVHNRLEQLHEIFAYNLYP
                     GPSAKKGMYAALLDKGEKEGWVTTHCSSVQAISPYDNTTAFMHEGASGGGKSEMMQHL
                     ARELNGQVMIGKNIVTGETRAISLPIFCSFNPITDDMALCHPSFQKTPGKLSISDAEN
                     GWFIRVDSVCDYGDDPHLEKQTIKVQKPLLFLNIQSHPGGTALIWNHTEDEPGKPCPN
                     PRVILPRETVPGSIAKPVAIDIRSFGVRTPPCSSENPNYGIFGLFHILPPALAWLWRL
                     VSPRGHANPSIIGGDGMGSEGVGSYWPFATGKRVTHANLLLGQIMQTPKMRYTLVPNQ
                     YIGVWKVGFKPQLLMREYLTRRGNGNLKPEQYQPARCSLLGYELNFLSIEGAKIPSRF
                     LQVYKQPEVGFEGYDKGADILREFFKKELPQYLTPELHPVGRKIIEACLADASVEDYN
                     DIIPFI"
     gene            complement(2614..>2918)
                     /locus_tag="PLD76_05165"
     CDS             complement(2614..>2918)
                     /locus_tag="PLD76_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU27425.1"
                     /translation="ATYYCPHTTEDNCFCKKPSPYFINQAVHEYNIDRKHSYIIGDHP
                     SDSECGIRAGLTPIYVLTGHGNKHKNELTCEVAICKSLSHATERILNNIYYDNKII"
BASE COUNT          842 a          559 c          500 g         1017 t
ORIGIN      
        1 ctactgccat agggttgagt gcattagccc ccaatccata tactgtttca gtaggaaatg
       61 caactaaccc tccttcgcgt attatcagtg ccgcttgttt aatattttct ccaataatca
      121 tttttttagg cttgactttt ttgcaaaaga gcatctactt cttctaattc ttcacgtttt
      181 ttttcttgaa aagctgctat cttttcacgc aatgatggat ttgataatcc aaatattttg
      241 gctgctgcta acgctgcatt ttctggtgat aaaacagtca gaggagcaac acccgaaggc
      301 atacgaacac tagagaaaat atcagtgcca ccaaatttat cactatatgg tgggcatgct
      361 attacaggac aataggtttg tgcatcgcta aatccactca acgcattgct catgcctgca
      421 atagtaataa agattacatt ttctttttca tattctttaa ttaaagcata gcatttaagt
      481 ggtactttat gtgcagatgc tatacggatt acagatggaa tttcaaatct atctaatact
      541 gttttaattt gtgttcccca gtctaaatcg gcttttgagc ccattaaaat aattactttc
      601 atgtcgttat ttgattgtgt tatgttacgt ttaaaattat ttttcttata taaaatcgcc
      661 tatgcgttga aataatgttt tgtttttcaa actcatttca ggcataggcg atatgttatc
      721 catagataga tattgctcta ttaaataaac ggaataatat cgttgtagtc ttctactgat
      781 gcatcagcca agcaagcttc aataattttt cttcctacag gatgtaattc tggagttaag
      841 tattgaggta attctttttt aaagaattct ctcaaaatat ctgctccttt atcatatcct
      901 tcgaatccaa cctcaggttg tttgtatact tgcaagaaac gagatggaat ttttgctcct
      961 tcaatagaaa ggaaatttaa ttcatatcct aacagagaac aacgagctgg ttgatattgc
     1021 tctggtttta agtttccgtt tcctcttctg gtcaagtatt ctctcattaa taattgaggt
     1081 ttgaatccta ctttccatac accaatgtat tgattcggta ccaatgtata acgcattttt
     1141 ggagtctgca ttatttgccc taacaaaaga ttagcatgtg ttactctctt accagtggca
     1201 aagggccaat atgaacctac accttcagaa cccattccat ctccacctat aatacttggg
     1261 ttagcatgtc cacgtggaga aactaatctc cataaccatg ctaatgcggg tggaagtata
     1321 tggaataatc caaatatacc gtagttagga ttttcgctag aacatggtgg agtacgtaca
     1381 ccaaaactac gaatatcaat agctactggt ttggcaatgc taccaggcac agtttcgcga
     1441 ggtaaaataa cacgaggatt tggacatggt ttgccaggtt catcttctgt gtgattccaa
     1501 ataagagcgg tacctcctgg gtgtgattga atatttaaaa ataatagagg tttttgtact
     1561 ttaatagttt gtttttccaa gtgaggatca tctccatagt cgcatacact atctacacga
     1621 ataaaccagc cattttcagc atcgctaatt gataatttac caggggtttt ttggaaagat
     1681 gggtgacaca atgccatgtc atcggtaatt gggttaaacg aacagaatat tggtaaactg
     1741 atagcacgag tttctcctgt tacaatgttt tttccaatca ttacttgtcc attcaattcc
     1801 ctagctaaat gctgcatcat ctcacttttt ccaccaccac ttgccccttc gtgcataaat
     1861 gcagtagtat tatcgtatgg gctaattgct tgtacacttg aacagtgtgt agttacccag
     1921 ccttcttttt ctcctttgtc taatagagcg gcatacattc cttttttagc actaggacct
     1981 ggataaaggt tataagcaaa aatttcatgt aattgctcta aacggttatg aacaactact
     2041 tgttttcctt caaaatgggt atgtctaaaa actggagcga catatagaac agactcaatt
     2101 gcgaatgagt cttccatttc attaattggt acaatatgtt gtaacattgc caaccccata
     2161 gcaaagaaac cagcatttgc aggcacgata gctataccgg gtacgccaat gccattattt
     2221 cctgcaaaat aaaagaaaat agccaattct tgtgttttta gccaatcaaa agtttctttt
     2281 cttaaattag aaaattcata tccgtatttg tctttaaaac gttttttgtc tgttggtaaa
     2341 ttatcaccta tagccattgt gttagggtcg cgacgacgca tataggcttc agtgtaattg
     2401 gcagaaatac catttgttac acgatgaaca atagcttcag tgtattcacc tttgcctggc
     2461 acgtcgtatt ttacttcaaa ttctagcttg tcttttccac ctacagcagc atctgctatg
     2521 tcttcaacag tttcaaataa ggtaaaccct ttgcagctgt ttagcagaat ctgtacgtca
     2581 tctggtaggg ttacattttt ttcttgtaat cttttaaata attttgttgt cataatatat
     2641 attgtttaaa attctctcag ttgcatgaga tagacttttg catatggcta cctcacatgt
     2701 taattcgttt ttatgtttat ttccgtgacc tgttaataca tatataggtg ttaatcccgc
     2761 tcgtataccg cattcactat cactaggatg gtctccaatt atataagaat gctttcggtc
     2821 aatattgtat tcatgtactg cttgattaat aaaataagga cttggttttt tgcaaaaaca
     2881 attgtcttca gtggtgtgcg gacaataata agttgccg
//
LOCUS       DAOSMY010000206         2913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_34131_length_2913_cov_3.178446, whole genome shotgun sequence.
ACCESSION   DAOSMY010000206 DAOSMY010000000
VERSION     DAOSMY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34131_length_2913_cov_3.178446"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLD76_05170"
     CDS             <1..254
                     /locus_tag="PLD76_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU27426.1"
                     /translation="LLAGMGFVAVFAGATNTPLACTMMGIELFGAESGLYIGIACVVA
                     YFFSGHTGIYAAQKVGSPKHTAFLKQQGESEAETRSVRS"
     gene            complement(257..790)
                     /locus_tag="PLD76_05175"
     CDS             complement(257..790)
                     /locus_tag="PLD76_05175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOU27427.1"
                     /translation="MNILAFGASHSKSSINQQFASYVAQLFSAGNQVSVIKLTDFDAP
                     VYTVDREAESGFPEAVKQFLLQLQKADLIIISLAEHNGSYTASFKNLFDWTSRVEMNL
                     FANKKLFLLSTSPGPKGGKFVMEAAKVRFPIHGAEIVGSFSLPRFADNFDAQKGITNT
                     ELKEAFDTEIEKVTTAL"
     gene            complement(879..>2913)
                     /locus_tag="PLD76_05180"
     CDS             complement(879..>2913)
                     /locus_tag="PLD76_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU27428.1"
                     /translation="VTANFSKNKPINDLALVSSRSFSVEETERFAGSLGDPARMVANY
                     AGVVVGNDSRNDIIIRGNSPSGLLWRMEGVAISNPNHFGAHGTTGGPVTMLNSNLLAN
                     SDFMTGAFSAEYGNALSGVFDLNLRTGNTERFEGMGQVGFNGFELLLEGPIKIGNFIK
                     NGSFIADYRFSTLQLMSLLGFDIGTGGTVPEYNDLSVMIDLPTSKIGRFKLIGLLGNS
                     FISMGRSFDIDEAITHNQMGYATDFGAGLNIAMLTHTYMFNEHSRLKSSLSFEHATST
                     TLSDSINYTDKKYFNLYAANYKENKWMANTQFKRRINAKNNLTLGASASLFVSSFFDS
                     VFVKSLDKRITLTAIDKEPSILYNAYTNWQHKFTNELSLNTGLHYQLYNLTNESAIEP
                     RLALRWQAHQQHAFSLGYGLHSQIQARTIYFYEAYYKDTDTYARNNMTTGYTRSHHIV
                     GGYDWTISSYWRVKTEAYYQDLFNVPVSPISGSYSALNSGSGYYIDIIDSLQNTGVGR
                     NYGLEMTIEKFLHKGYYGLMTFSLYDSKYQGYDKIWRNTAFNSNFAVNLLGGYEWRVG
                     TKNFLAVDVRTIWAGGMRYVPIDLAASIAAGEQRLASTDIYTQRFNDYFRCDVRISFK
                     QNLGKVSQEWGLDLQNVSNYKNIFAQQYNKYSESIATVYQQGFMPMMMYRIHF"
BASE COUNT          866 a          601 c          575 g          871 t
ORIGIN      
        1 cattgcttgc aggaatggga tttgtggctg tgtttgcagg agcgactaat acgccgcttg
       61 cctgcaccat gatgggaata gagttatttg gagcagaaag cggtttgtat atcggtattg
      121 cttgcgtggt ggcttatttc ttttcgggtc atacaggtat ttatgctgcc caaaaagtgg
      181 gaagcccaaa gcatacagca tttctcaagc aacaaggaga aagtgaggct gaaacccgtt
      241 cggtgcgatc gtaggtttac aaagctgttg ttactttttc aatttctgtg tcaaacgctt
      301 ctttaagttc cgtgttcgta atgccttttt gtgcatcaaa attatccgca aaacgaggca
      361 atgaaaaact tccaacaatt tctgccccat gaatggggaa acgtactttg gctgcttcca
      421 ttacaaattt tccaccttta ggtccgggag aagtagaaag taaaaataat tttttgttgg
      481 caaaaagatt catttctaca cgagaagtcc aatcgaacaa gtttttgaac gatgcagtat
      541 aactaccatt gtgttcggct aaggaaataa taatcaaatc tgccttttgt aactgaagta
      601 aaaactgttt tactgcttcg ggaaaaccac tttctgcttc cctatctacc gtatatacag
      661 gagcatcaaa gtcagtaagc tttataacag acacttggtt ccctgccgaa aataactgtg
      721 caacatagct tgcaaattgt tggttgatgg agcttttgct atggctagct ccaaatgcta
      781 gtatattcat gatgtgctat tttttagttt atgaagcaaa aatagcaatt taacaatgcg
      841 tttccaattc ttttattaac atttctgctt ccttaacatt aaaaatgaat tcgatacatc
      901 atcataggca taaagccttg ttggtaaacg gtagctatac tttctgaata tttgttgtat
      961 tgttgtgcaa aaatgttttt ataattgctc acgttttgca aatccaatcc ccattcttgc
     1021 gatactttgc ctaagttttg tttaaaacta attcgcacat cgcacctaaa ataatcgttg
     1081 aagcgttgcg tataaatatc agtagaagct aaacgttgtt cgcccgctgc aatagaggca
     1141 gccaaatcga taggcacata ccgcataccg cctgcccaaa tggtgcgaac gtcaacagcc
     1201 aaaaagtttt ttgtaccaac acgccattcg taaccaccca ataagttgac agcaaaattg
     1261 gagttaaaag ctgtatttct ccatatttta tcataccctt ggtattttga gtcgtaaagc
     1321 gagaaggtca ttaaaccata ataccccttg tgaaggaatt tttctatggt catttccaat
     1381 ccataatttc gtccgacacc cgtattttgc agactatcaa taatgtcaat gtagtagcca
     1441 ctacccgaat ttaaggcaga ataactgcca ctaatgggcg aaacaggcac attgaataaa
     1501 tcttggtaat acgcctccgt ttttacacgc caatacgaag aaattgtcca atcgtaaccg
     1561 cccacaatgt gatgcgatcg cgtgtaacca gtagtcatat tattgcgagc atacgtatcg
     1621 gtatctttgt aatacgcctc ataaaaatat atggtacggg cttgtatctg actgtgcaat
     1681 ccgtaaccca aagagaaagc gtgttgctga tgagcttgcc aacgtagagc aaggcgaggt
     1741 tcaatggcac tttcgtttgt taaattatac aattgataat gcaagcctgt attgagcgac
     1801 aattcgttgg taaacttatg ttgccaattg gtatacgcat tgtacaagat gcttggttct
     1861 ttgtcaatgg ctgttagggt aatgcgttta tccaaacttt taacaaatac cgaatcaaaa
     1921 aaacttgata caaaaagact agcagaagca cccaaggtaa ggttgttttt tgcgtttatc
     1981 ctgcgtttaa attgtgtgtt tgccatccac ttattttctt tgtaattggc ggcatatagg
     2041 ttgaaatatt ttttgtcggt atagtttatc gagtcagaca gcgtggtaga agtagcgtgc
     2101 tcgaaagaaa gactgctttt taaacgggaa tgttcgttga acatatacgt gtgggttaac
     2161 atcgcaatgt tcaatcctgc accaaaatcg gtagcatatc ccatttgatt gtgggtaatg
     2221 gcttcatcta tatcaaaaga gcgtcccatc gaaataaaac tattgcctag taaaccaatc
     2281 agcttaaagc gtccaatttt gctggttggc aaatcaatca ttaccgataa gtcgttgtat
     2341 tcgggcacag tacctcctgt acctatatca aaacccaaca ggctcattag ttgcaaggta
     2401 gaaaaacgat aatcggcaat aaacgaacca tttttaataa aattaccaat ttttatggga
     2461 ccctcgagta gcaactcaaa accattaaag cctacttgtc ccattccttc aaaacgttcg
     2521 gtattacctg tgcgtaggtt taaatcgaat acacccgaca aggcattgcc atattcagca
     2581 ctaaacgccc cggtcataaa atcagaattt gccaataagt tgctattgag catggtaaca
     2641 ggtccgccag tagtgccgtg tgccccaaag tggttggggt tggatatggc tacaccctcc
     2701 atacgccaca acaaacccga aggcgaattg cccctgatga taatatcatt gcgtgagtcg
     2761 ttgcctacta ccacacctgc ataattcgct accatacggg caggatcgcc cagactgcct
     2821 gcaaagcgtt ctgtttcttc tacactaaac gaacgtgaac tcaccaacgc caagtcgtta
     2881 atgggtttgt ttttagaaaa attggctgta acc
//
LOCUS       DAOSMY010000207         2859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_35001_length_2859_cov_2.719686, whole genome shotgun sequence.
ACCESSION   DAOSMY010000207 DAOSMY010000000
VERSION     DAOSMY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35001_length_2859_cov_2.719686"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLD76_05185"
     CDS             <1..957
                     /locus_tag="PLD76_05185"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOU27429.1"
                     /translation="SLLHTKGITLHTYTDQVIFAPGEVIKADGSPYTIFTPFSKIWLR
                     KYEQLPHQEYTTDAYKKNWLSTPAQEIPSLAKCGFKKTSIQYTPPSIPYSIIDNYTDT
                     RNFPAINGTSTLSIHLRFGTISIRQLARIAFTRNHTYVSELIWRDFFMQILYFFPHVQ
                     TNAFKPKYDAIEWRNDTAEFSRWCNGTTGYPLVDAGMRQLNETGWMHNRVRMVVASFL
                     CKHLLVDWRWGEAYFATKLMDYDLSANNGNWQWAAGCGCDAAPYFRVFNPTEQAKKFD
                     SNSEYIRQWIPEVDTNKYATPIVEHTFARNRAIETYKKAISG"
     gene            1048..>2859
                     /locus_tag="PLD76_05190"
     CDS             1048..>2859
                     /locus_tag="PLD76_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU27430.1"
                     /translation="MILATIFETTLPLTNPVLKFLLILIIILAAPIFLNKIKIPHLLG
                     LIIAGAIIGPFGLNLIERDSGIILSGTAGLLYIMFLAGLEIDIADFKKNTSRSLVFGM
                     YTFIIPMVLGILGSIFILQFSITTSVLLASMFASHTLIAYPLISKLGVVKNKAVTMAV
                     GGTMITDTLALLVLAVIVGMANGEVNSDFWIRLSVSLLAFGLIITILFPIIARWFFKK
                     FDDNVAQYIFVLAMVFLGATLAELAGIEAIIGAFLTGLALNRLIPHTSPLMNRVEFVG
                     NAIFIPFFLIGVGMLVDYRAFFKDWETLKVAAVMTIIATLAKFLAAWLTQKTYKLSLD
                     ERRIIFGLSNAQAAATLAAVLIGYNLQIFDASVLNGTILMILVTCTIASFQAQKGAKN
                     IALSEDLKSSTGEVETDERILIAINQENNLEELVNLGVTIKSKHNKKGLYALHIIDND
                     ESQNNNEKQAKKLLQQAGRIAAATDTKLNELLRYDINIVNGITNSLKEQRITDLILGL
                     NNKKGISDGFFSNLTLGILSKSNPTTLIYKPTQPFGTIKRHLVIVPDKAEKEIGFPFW
                     IIKTWNIARNSGAKIVFYSSIDTQKVIREIQSKHPIEC"
BASE COUNT          965 a          531 c          511 g          852 t
ORIGIN      
        1 tcgttgttgc atacaaaagg aatcacctta catacctata ctgatcaagt tatttttgca
       61 ccaggcgaag taataaaagc agacggaagt ccttatacta tttttacgcc tttttcaaaa
      121 atatggctac gtaaatacga gcaactacct catcaagaat ataccactga cgcatataag
      181 aaaaattggt tgtcaacacc tgctcaagaa ataccatcac tcgcaaaatg tgggtttaaa
      241 aaaacgagca tacagtacac accccccagt atcccctact cgattattga caactataca
      301 gatacacgaa attttccagc cataaatggc acttcaacac taagcatcca tctacgcttt
      361 ggaaccataa gcatccgaca attggcacgc atagctttta cacgtaatca tacatatgta
      421 agcgaactga tttggcgaga tttttttatg caaatcttgt atttttttcc acacgtacaa
      481 acaaatgctt ttaaaccaaa atacgatgct attgaatgga gaaacgatac cgcagaattc
      541 agtcgatggt gcaatggaac tactggatat ccattggtag atgcaggaat gcgtcaacta
      601 aacgaaacgg gctggatgca caacagagta agaatggtag tagctagttt tttgtgcaaa
      661 cacttattgg tagattggcg ttggggcgaa gcgtattttg ccaccaaatt gatggattac
      721 gatttatctg ccaataatgg caactggcaa tgggctgcag gttgtggctg cgatgcggca
      781 ccctatttta gggtttttaa ccccaccgag caagcaaaaa agtttgattc aaacagcgaa
      841 tacattcgtc aatggatacc tgaagtagat acaaacaagt acgcgacacc catcgttgag
      901 catacatttg cacgtaaccg ggctatcgaa acgtataaaa aagctatatc agggtaaatt
      961 ctcacaaaaa aggcttaaaa aggtaaaaaa aagctatata aagtagaata tttttttcta
     1021 tctttgccac aaatcaacac accacctatg attttagcca cgatatttga aacaacacta
     1081 ccattaacca atcctgtgtt gaaatttttg ttaattctta tcatcatctt agccgcaccc
     1141 atattcttaa ataaaataaa aatccctcat ttgcttggat taattattgc aggagccatc
     1201 attggtcctt ttgggttaaa tttaatcgaa cgtgatagtg gaatcattct ttcagggaca
     1261 gccggtttgc tatatatcat gtttttagca ggcttagaaa tagatattgc tgattttaaa
     1321 aaaaacacaa gtcgtagtct tgttttcggg atgtacacgt ttatcattcc tatggtatta
     1381 ggcatattgg gcagtatatt cattcttcaa ttttctatta ccacatcggt acttcttgcc
     1441 agtatgtttg cttcccacac ccttattgcc taccccttaa ttagcaaatt gggtgtagta
     1501 aaaaataaag ctgtaacaat ggcagtagga ggcacgatga taacagacac acttgcttta
     1561 ttggtattgg cagtaatagt tgggatggca aatggcgagg ttaatagcga tttttggatt
     1621 cgcttatcag tttccctttt agcatttgga ttaattataa ctatcctatt tccaataatt
     1681 gcacgatggt tttttaagaa atttgatgat aatgtagccc aatatatttt tgtgttagca
     1741 atggtctttt taggagctac tttagctgaa ttagctggta tagaggctat tattggggca
     1801 ttcttaacgg ggttagcttt aaatagactc ataccacata cttctccgct catgaataga
     1861 gtcgaatttg tgggaaatgc catttttatt cctttcttct taatcggtgt aggaatgttg
     1921 gttgattacc gagcattttt taaagattgg gaaaccttaa aagtagcagc agtaatgact
     1981 attatcgcta cattagcaaa atttttagca gcttggctta ctcaaaaaac atacaaactt
     2041 agtcttgatg aacgaagaat tatttttgga ttaagcaatg cacaagcagc agccacctta
     2101 gccgcggtat taattggata caatcttcaa attttcgatg ctagtgtgct aaacggaact
     2161 attttgatga tattagtaac ctgtacaatt gcatcttttc aagctcaaaa aggagcaaaa
     2221 aatattgctt tatccgaaga tttaaaatct tcaacgggtg aagttgaaac agacgaacgc
     2281 attctaattg ccattaacca agaaaacaat ttagaagaat tggtaaattt aggggtaaca
     2341 attaagtcaa aacacaacaa aaaagggctg tatgctctac atattattga caacgacgaa
     2401 tcacagaaca ataacgaaaa acaagcgaaa aagctgttac aacaagcagg acgtattgct
     2461 gctgcaactg atactaaatt aaatgaacta ttgcgctacg atattaatat agtaaatggt
     2521 atcacaaata gcctaaaaga gcaacgcatc accgatttaa ttttaggctt aaacaataag
     2581 aaagggattt cagatggatt ttttagcaac cttactttag gcatattgag caagtcgaac
     2641 cctaccacct taatttataa accaacacaa ccttttggca ctatcaaacg ccatttagta
     2701 attgtacctg ataaagctga aaaagaaata ggatttcctt tttggattat caaaacatgg
     2761 aatatcgcac gcaattcggg agcaaagatt gtcttttact caagtataga tacccaaaaa
     2821 gtaattcgag aaattcaatc caaacatcca atcgaatgc
//
LOCUS       DAOSMY010000208         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_35185_length_2847_cov_2.932307, whole genome shotgun sequence.
ACCESSION   DAOSMY010000208 DAOSMY010000000
VERSION     DAOSMY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35185_length_2847_cov_2.932307"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="hcp"
                     /locus_tag="PLD76_05195"
                     /gene_synonym="priS"
     CDS             complement(<1..1599)
                     /gene="hcp"
                     /locus_tag="PLD76_05195"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOU27431.1"
                     /translation="MFCNQCQESAHNTGCTIKGVCGKTEDTAGIQDLVVYVCQGLAYV
                     NIEARKQGVNTLPESRKIAQSLFATITNANFDNSLLIKQIKEVIELRNSLHNKLNTPL
                     KHHTVRWNGTTDIEFVAKAALVSVQTYDADIDIRSLKQYVLYGLKGMAAYAEHAANLG
                     QEDGDIYDFIEKALVMITKETSLDDMLSWVMKTGEYGVKVMSLLDAANTNTYGNPEIT
                     KVNIGVGKKPGILVSGHDLKDLEELLEQTKNSGIDIYTHSEMLPSHAYPMFKKYPHLV
                     GNYGNAWHKQLEEFESFNGPILFTTNCLVPPRKSTTYNNRVFTSGATGMPEWKHIDTH
                     LPNGQKDFTEIISLAKTCPPPTEIETGELTIGFAHNQVLALAGTILENIQSGNIKKMV
                     VMSGCDARQKSRQYYTDFAEALPSDTLILTSGCAKYRYNKLPLGDINGIPRVLDAGQC
                     NDSYSWAIVALKLKEVLGLEDINDLPLVFNIAWYEQKAIIVHLALLYLGVKDTHIGPT
                     LPAFMTPNVLKLITEKFGVKTNATV"
     gene            complement(1637..2491)
                     /locus_tag="PLD76_05200"
     CDS             complement(1637..2491)
                     /locus_tag="PLD76_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOU27432.1"
                     /translation="MLRTIIKIDEELCNECEACVQGCHEGALQMIDGKARMVSELFCD
                     GLGACIGECPVGAITLEEREAEPYDEIATIERMIPKGENTILAHLKHLKEHGEFDYLK
                     QAVSYLRENGVKVDFAQVHNMPIHGKEESKGCPGSREVSFANTTPSFRVDTNTPATSA
                     LRQWPVQLHLLNPQAGYFKSADVVLAADCVAFSYANFHQKFLAGKTLAIACPKLDSNK
                     EIYVEKLQSMIEESLINTLTVIIMEVPCCGGLLSLASKAVSQSNRKIPMKLIVVSIKG
                     EILKEEWV"
     gene            complement(2497..2826)
                     /locus_tag="PLD76_05205"
     CDS             complement(2497..2826)
                     /locus_tag="PLD76_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU27433.1"
                     /translation="MEKKEFPKAQVFTFVDSVDYAAGSIVSKILLRNEKGNLTLFAFD
                     KGEFLSEHTAPFDAIVQILDGTAEVIIDKKPHLLTTGQSIIMPANVSHALTATERFKM
                     LLIMIKG"
BASE COUNT          743 a          543 c          533 g         1028 t
ORIGIN      
        1 tacggttgca tttgttttta ctccaaattt ttctgttatc agttttaata cattcggagt
       61 cataaaggcg gggagagttg gtccgatatg tgtgtctttt actcctagat ataacaaagc
      121 aaggtgtaca ataatggctt tttgctcgta ccaagcaata ttaaacacca atggtaaatc
      181 attaatatct tctaaaccaa gcacttcttt tagttttaaa gctacgattg cccatgagta
      241 ggagtcgttg cactgaccag catccaatac gcgaggaatt ccgttgatat ctcctaaagg
      301 tagtttgttg tagcggtatt tggcacagcc agaggttaat attaaggtgt ctgatggcag
      361 ggcttcggca aaatctgtgt agtattggcg tgatttttga cgtgcatcgc aacccgacat
      421 gacaaccatt tttttgatgt tgcccgattg aatgttttct aaaatggtgc ctgctaatgc
      481 caatacctga ttgtgggcaa agccgatggt taattctcct gtttctatct cggtaggtgg
      541 tgggcaggtt tttgctaaag aaattatttc agtaaaatct ttttgcccgt ttggtaaatg
      601 agtgtcgata tgtttccact ctggcatacc tgtagcacca gatgtaaaca ccctattgtt
      661 gtatgtggta gattttcggg gaggaactaa acaattggtt gtgaatagta taggaccatt
      721 aaacgattcg aattcttcta attgcttgtg ccaagcgttt ccataattac ctactaagtg
      781 aggatatttt ttgaacattg gataggcatg tgagggtaac atctcagagt gagtgtaaat
      841 gtctattccg ctgttttttg tttgttccaa tagctcttct aaatctttta aatcatgtcc
      901 gcttactaag atgcctggtt tttttcctac accaatattt accttggtta tttctggatt
      961 gccataggtg tttgtatttg ctgcatctaa taatgacatt acttttacac catattcgcc
     1021 tgttttcatt acccaagaaa gcatatcgtc gaggcttgtt tcttttgtaa tcattacgag
     1081 agctttttca ataaaatcat aaatgtcgcc atcctcttgt cccaaattag cggcatgttc
     1141 ggcatacgct gccatacctt ttagtccgta caatacatat tgttttagcg aacgtatatc
     1201 aatatctgca tcgtaggttt gtacacttac tagagcagct ttcgcaacaa attcaatgtc
     1261 agttgtgcca ttccacctta cagtatgatg tttgagagga gtgtttaatt tattatgaag
     1321 cgaatttcga agttcaatta cttcttttat ttgctttatt agtaagctat tatcgaaatt
     1381 cgcgtttgta attgttgcaa acaaactttg tgctattttt ctactttcgg gtagggtatt
     1441 tactccttgt tttcgtgcct ctatgtttac gtatgctaaa ccttgacata catatactac
     1501 taaatcttgg ataccagcag tatcctcagt tttaccacat acacctttta tagtacagcc
     1561 agtgttgtgt gctgattctt gacattgatt acaaaacatg ctcatattat tatcgtgtta
     1621 aatgtttatt tttgagttat acccattctt cttttagtat ttctcctttt atactcacta
     1681 caattaattt cattggtatt tttctgtttg attgcgatac cgctttgctt gctaaagaaa
     1741 gcaatccacc gcaacaagga acttccataa tgataactgt aagggtattg attaacgatt
     1801 cttctatcat cgattgtagt ttttctacgt atatttcctt attgctgtct aattttggac
     1861 aggctatggc taaggttttt cctgctaaaa atttttgatg aaaattggcg taactaaatg
     1921 caacgcaatc agcagcgagc acaacgtcag ctgatttaaa atatccagct tgtgggttta
     1981 gaaggtgcaa ttgcacgggc cattgcctta gtgccgaagt ggcaggggtg ttggtgtcaa
     2041 cacgaaaact tggcgttgtg ttggcaaaag atacttctcg actgcctgga caccctttgc
     2101 tttcttcttt gccatgtatg ggcatgttgt gtacttgtgc aaagtctact tttacaccat
     2161 tttcgcgtaa ataggaaaca gcttgtttta agtagtcaaa ttccccatgc tcttttagat
     2221 gctttaagtg agctagaata gtgttttcgc cttttggaat catacgttca atggttgcaa
     2281 tttcatcata aggttcagct tctctttctt ctagtgtaat ggcacctaca ggacattcgc
     2341 caatacaagc tcctagcccg tcgcaaaata attcgcttac catacgtgct tttccgtcaa
     2401 tcatttgcaa ggcaccttca tggcaacctt gcacacaggc ttcgcattcg ttacacaatt
     2461 cctcgtcaat tttaataatt gttcttaaca tagtgcttat ccttttatca tgatgagtaa
     2521 cattttaaaa cgttcggtag cagttaaggc atggctcaca tttgcaggca ttataatact
     2581 ttgtcctgtt gttagtaaat gtggcttttt atcaataata acttcagctg taccatcaag
     2641 tatttgtaca atggcgtcaa atggtgcagt gtgctcactt aaaaactcgc ctttatcaaa
     2701 agcaaatagg gtaaggtttc ctttttcgtt gcgtagaagt attttactaa caatagagcc
     2761 tgctgcataa tcaacactat ctacaaatgt gaacacttgt gcttttggga attctttctt
     2821 ttccatacta tcatcttttt attttgt
//
LOCUS       DAOSMY010000209         2847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_35186_length_2847_cov_2.777937, whole genome shotgun sequence.
ACCESSION   DAOSMY010000209 DAOSMY010000000
VERSION     DAOSMY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35186_length_2847_cov_2.777937"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /gene="rodA"
                     /locus_tag="PLD76_05210"
     CDS             complement(<1..1268)
                     /gene="rodA"
                     /locus_tag="PLD76_05210"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOU27434.1"
                     /translation="MSRKVSLFRSLDWITILLYVTLVTIGWFAIYSSTYDFENPTILN
                     FSARHGSQLIWIGFAFLLALITILIDSKMYNNLAYIIYFLIILLLIATIFLAPEIKGS
                     RSWLKIGPITMQPAELAKYATALCLGKFMSSYNFTLNSIKNMFLVGFIILTPMLIIIL
                     QQETGSALVFAAFFLVLFREGMNGIFMVLAFLGALFFVLFLRYDYISSSSNSYILESF
                     GFVIVFILSVISVGIMIAYYLKDNRQMLQFIGGNAILYLLTGILHLFGIEAITYTITA
                     YISISLSILYLFIMALLQKQKKYFLFIIFLIGSIALCNVTDYFFNNVLQPHQQMRIKV
                     VLGLEDDPLGAGYNVRQSKIGIGSGGLLGKGFLNGTQTKLNYVPEQDTDFIFCTIGEE
                     QGFVGSIVVLFLYIALIIRLVILAERQRSS"
     gene            complement(1281..>2847)
                     /locus_tag="PLD76_05215"
     CDS             complement(1281..>2847)
                     /locus_tag="PLD76_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU27435.1"
                     /translation="NINQEEFDNRIEEIKDKRKNRGYSRFTPQIFMSQLSVAEYGKIQ
                     EQLYKFRGVYAQQRVLRKYTTPHGAHAIGSVGEVNQQQMDADSYYSRGDYKGQSGVEK
                     EYETYLRGEKGVEILLRDAHGRIQGVYKNGERNKAPIAGKNLTMAIDMGLQEYGEQLM
                     IKKLGSIVAIEPSTGEILALISSPSYDPHLLVGRERSKNYNALLKDPFKPLFDRPLMA
                     MYPPGSTFKLVNGLIYQQEGVITEDTRFSCSMGYYYSRTRKLGCHPHPNNLDLRESVQ
                     HSCNAYYCHGLKGLLDSPPSGISTTESYNHWRNHVLSMGFGKKLGVDFPNEKGGLILE
                     SNRYDRIYGKNKWKSSTIISIAIGQGEVLATPIQTANLAALIANRGYFYTPHIIKKIE
                     GDSIDKRFREKHQTTIEQKYFDPIIDGMLMAVEQGTGRVGRIEGIEICGKTGTAQNPH
                     GKDHSIFMAFAPKENPKIAIAVYVENSGFGATWAVPIASLMIEKYLTGTVDTTKLNRK
                     NLQKRMLETFLIE"
BASE COUNT          920 a          516 c          444 g          967 t
ORIGIN      
        1 aaggaagatc gctgtcgttc agccaaaatt acaagtcgta tgattaatgc aatatataga
       61 aataagacca ctatacttcc cacaaatcct tgttcttccc caattgtgca aaaaatgaaa
      121 tctgtatctt gttcgggaac ataatttaat tttgtttgtg ttccatttag aaatcctttt
      181 ccaagcaacc cacctgaacc aatgccaatt tttgattgac gaacattgta tccagcaccc
      241 aatgggtcgt cttctaatcc taatactact ttaatacgca tttgctgatg aggttgcaac
      301 acattgttga aaaaataatc agtaacattg cacagggcta tacttccaat cagaaatatg
      361 ataaaaagga aatacttctt ttgcttttgt aataaagcca ttatgaataa atagagaata
      421 cttaaagata tacttatata agcagttata gtatatgtaa tagcttcgat tccaaataga
      481 tgtaagattc ctgtaagtaa atataatata gcattaccac caataaattg taacatttgc
      541 ctattatctt ttaggtaata agcaatcatt atgcccaccg aaataacaga caaaatgaaa
      601 acgatgacaa aaccaaacga ttcgagtata taggagttac tactcgacga aatataatca
      661 tatcgtaaaa aaaggacaaa aaataaagct cctaagaagg ctagtaccat aaaaattcca
      721 ttcatgcctt ccctaaacaa tacaagaaag aatgctgcaa aaaccaatgc agatccagtt
      781 tcttgttgga gtatgatgat taacatagga gttaaaataa tgaatccaac aagaaacata
      841 tttttgatgg aattaagtgt aaaattgtat gaactcataa attttccgag acatagagct
      901 gtagcatatt tagctaattc cgcaggttgc atcgtaattg gtccaatttt tagccatgag
      961 cgagaccctt taatttctgg ggctaaaaaa atggtggcaa ttaacagtaa aataattaga
     1021 aaataaataa tataagcaag attattgtac attttgctat ctatcaatat cgttataagt
     1081 gccagtaaaa aagcaaaacc aatccaaatt agttgggagc catgtctagc actaaaattc
     1141 aggatagttg gattttcaaa atcgtatgtt gatgagtaga tagcaaacca tcctatagtt
     1201 accaatgtaa catacaataa aatggtaatc caatctaatg aacgaaaaag actaacttta
     1261 cgagacatag ttgatatttt ttattcaatt aaaaatgttt ctaacatcct tttttgaaga
     1321 tttttacggt ttaattttgt tgtatctaca gtacctgtaa ggtatttttc tatcattaaa
     1381 ctagctatcg gtactgccca tgttgctcca aaacccgaat tttccacata tacagctatt
     1441 gctatttttg gattttcttt cggagcaaaa gccataaaaa ttgaatgatc ttttccgtgt
     1501 ggattttgtg ccgttcctgt tttaccacaa atttcaatac cttctattct gccaacccta
     1561 cctgttcctt gttccacagc cattaacatt ccatcaataa taggatcgaa atatttttgt
     1621 tctattgtcg tttgatgttt ttctctaaaa cgtttatcaa tactatctcc ttcaattttt
     1681 ttgataatgt gaggagtgta aaaatatcct ctgtttgcta ttagtgctgc caaatttgct
     1741 gtttgaatgg gagttgctaa cacttctcct tgcccaattg cgatagagat aatagtggat
     1801 gatttccatt tgttttttcc gtatattcgg tcatagcgat tactttctaa aatcaaacct
     1861 cccttttcat taggaaaatc gactcctaat ttttttccaa aacccataga tagaacatgg
     1921 tttctccaat gattatagga ttcggtagta gaaataccac ttggtggaga atctaataaa
     1981 cctttcaatc catgacaata atacgcattg cacgaatgtt gaacagattc gcgtaaatct
     2041 aaattgttag gatgtggatg acagcctaat ttacgtgtcc gactatagta gtaacccatg
     2101 ctacaagaaa aacgtgtgtc ttcggttata acgccttctt gctggtagat taatccattt
     2161 actaacttaa atgttgagcc tggaggatac attgccatta ggggtctgtc aaataagggt
     2221 ttgaaagggt ctttcagcaa tgcattataa tttttagaac gttctcttcc taccaacaaa
     2281 tgagggtcat atgatgggct tgaaataagg gctaatattt ctcctgtaga gggttcaatg
     2341 gctacaatac tgcctaattt cttaatcatt agttgttcgc catactcttg taaccccata
     2401 tcaatagcca tggttagatt tttgcctgca attggagcct tgtttctttc tccattttta
     2461 tatacccctt gaatacgacc atgagcatct cttaataata tttctactcc tttttctcct
     2521 cttaaataag tttcatattc tttttctact ccagattgac ccttataatc acctctagaa
     2581 taatatgaat cagcatccat ttgttgctga tttacttctc ctacagagcc aattgcatga
     2641 gctccatgag gggtggtgta tttgcgtagt acacgttgtt gtgcatatac tcctctaaat
     2701 ttatacaact gttcttgaat tttgccatat tctgcaacac ttaactgaga cataaatatt
     2761 tgaggagtaa aacgagagta tcctctgttt ttacgcttgt cttttatttc ttctattcta
     2821 ttgtcaaatt cttcttgatt gatattg
//
LOCUS       DAOSMY010000210         2846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_35199_length_2846_cov_2.685417, whole genome shotgun sequence.
ACCESSION   DAOSMY010000210 DAOSMY010000000
VERSION     DAOSMY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35199_length_2846_cov_2.685417"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1817
                     /locus_tag="PLD76_05220"
     CDS             33..1817
                     /locus_tag="PLD76_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOU27436.1"
                     /translation="MSIVIQINKKKNCTLCWRLIFFCFALFLSQTAHAQANAEHISYS
                     RIYDFLDELANDGIIELNSSIKPYSKNFIASKLQEAQKADSLLTKRQKGDLVFFMQNY
                     ALFYQNFPTSHLPLVQKNAWNVALVEPAVHYKDNLVSAKITPLLGMHIYSNRHGAITH
                     RWFGADMYMNIGKNIAVYGSLRDNSYSGEYLSSLPVNAARLSQPAYLNDLPGVEYKEA
                     NYGGDFSDSRGGITLHNEWGSIGLIKDNVSWGDNYNGSNILSGRAPSFPMITMKLKPT
                     SWFELNYIHGFLISNVLDSSYYYIEQHTIDGEKKQYRPMNKFVAANMLTFTPIPKLNI
                     SVGNAIVYAERSIQPAYLIPIAFYKSIDHLLTKGIKTENQNSQFFINVSSRNIKHLHL
                     YGSMFVDEIKFSRFLPSNPETNPISYKGGFHLSNFPISNLSFTTEFTYSTMVNYKHSI
                     ESLTWASNSYNLGHYLGDNSQELFVSLQYKPIQKLSVQLAYMHALHGNEYLYIRRSVK
                     EVISQPVLQDITWSNNRLISKASYELWNNAYAIASVEYNHAKGYNVTSTPIEAEIRKD
                     AQGYLDMYSPAFYQGQNITYTIGFSLGF"
     gene            1847..>2846
                     /locus_tag="PLD76_05225"
     CDS             1847..>2846
                     /locus_tag="PLD76_05225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU27437.1"
                     /translation="MNMKKIQMVDLKSQYDKIKEEVNAGIQEVIDTTTFIKGGKVNVF
                     QKNLETYLHVKHVIPVGNGTDALQIALMALGLKAGDEVITPTFTFIATAEVIALLGLT
                     PVVVDVEEDTFNISIDALKKAITPKTKAIIPVHLFGQCANMDAIMEVANKHKLFVVED
                     ACQSIGSVYTFKDGRKVQSGCIGNIGCTSFFPSKNLGCYGDGGAIFTNDDTLAAAMRA
                     IANHGMVVRYYHDMIGVNSRLDSIQAAVLDVKLKNLDTYIANRQAAAKAYNKAFANHP
                     KITIPYEFPQSTHVYHQYTLKLKNVDRAALQKALEAKGIPAMVYYPVPLHMQKAYQD"
BASE COUNT          996 a          473 c          492 g          885 t
ORIGIN      
        1 gtcaatattg tatttaaaag tgctagaagt aaatgagtat agtgatacaa attaataaaa
       61 agaaaaattg caccctttgt tggcgtttga tttttttctg ttttgcctta tttttatcac
      121 aaactgcaca tgcacaagcc aatgctgaac acatctctta ttctcgcata tacgactttt
      181 tagatgaact ggcgaatgat ggtatcattg agttaaattc aagcataaaa ccatacagta
      241 aaaattttat tgccagcaaa ttgcaagaag cacaaaaggc agattctttg ctaaccaaac
      301 gtcaaaaagg tgatttggtg ttttttatgc aaaattacgc acttttttac caaaattttc
      361 ccacctctca ccttcctctt gtacaaaaaa atgcgtggaa tgtggcattg gtcgaacctg
      421 cagttcatta taaagacaat ttggtgtcag caaaaataac accattatta gggatgcata
      481 tctatagtaa tcgacacggt gctattacac accgttggtt tggtgctgat atgtatatga
      541 atattggtaa aaacattgct gtttatggaa gtttgcgtga taattcgtac agtggagaat
      601 acttatcttc gttacctgta aatgcagcac gtttatcaca acctgcttac cttaacgatt
      661 tgcctggtgt tgaatacaaa gaagcaaact atggtggcga ttttagtgat agtagaggag
      721 gaattacgtt gcataacgag tggggaagta ttggtttaat caaagacaat gtaagctggg
      781 gagataacta caacggttca aatatattat caggaagagc tccttcattt ccaatgatta
      841 caatgaaact aaagcccaca tcttggtttg aattaaatta catacatggt tttctgatta
      901 gcaatgtact agatagcagt tattactata ttgagcaaca taccattgat ggcgaaaaaa
      961 aacagtatcg ccctatgaat aagtttgtag ctgccaatat gctaactttc accccaattc
     1021 cgaaattgaa tatttcagtg ggtaatgcta ttgtgtatgc cgaaagaagt attcaacccg
     1081 catatttgat accaattgct ttctataaat caattgacca tctattaaca aaaggaatta
     1141 aaacagaaaa tcaaaactca caatttttta ttaatgtaag ttctcgaaat attaagcatt
     1201 tacatctata tggtagcatg tttgtggatg aaataaaatt ttcacgtttc ttgccttcaa
     1261 atccagaaac taatccaata tcatacaaag gtggattcca tttatcaaat ttccctattt
     1321 caaatctatc atttaccact gaatttactt acagtactat ggtaaattac aagcattcga
     1381 tagaaagttt aacatgggca tcaaattcgt ataatttagg tcattattta ggtgataatt
     1441 ctcaagaatt atttgtaagt ttgcagtaca aacctattca aaaactatcc gtacaacttg
     1501 cttatatgca cgcacttcat ggaaatgaat atttgtacat caggcgaagt gtgaaagaag
     1561 ttataagtca acctgtatta caagatatta cgtggagtaa caatcgacta ataagcaaag
     1621 caagttacga attatggaac aacgcttatg ccattgcaag tgttgagtac aatcacgcaa
     1681 aaggatacaa cgtaacatct actcctattg aggctgaaat aagaaaagat gcacaagggt
     1741 atttagatat gtattcgcca gcattttatc aaggtcagaa tattacctat actatcggat
     1801 ttagtttagg attttaaccg ttatttttat atactaatat caataaatga atatgaaaaa
     1861 gattcaaatg gttgacttaa aaagtcaata cgacaaaatc aaagaagagg tcaatgcggg
     1921 aattcaagaa gtaattgaca ccactacctt catcaaagga ggcaaagtga atgtatttca
     1981 aaaaaactta gaaacatacc tacatgtaaa acacgtcatt cctgtaggaa atggtactga
     2041 cgctttacaa attgcattaa tggcattggg attaaaagca ggtgatgaag ttattacgcc
     2101 tacctttaca tttattgcta ctgccgaagt cattgcttta ttaggattaa ctcctgttgt
     2161 agtagatgtg gaagaagata cttttaatat atctatcgat gctcttaaaa aagcaattac
     2221 accaaaaaca aaagctatta ttcctgtaca tttgtttgga caatgtgcta atatggatgc
     2281 aattatggaa gtagcaaata aacacaaatt atttgtagta gaagatgctt gtcagtctat
     2341 tggctctgta tatactttca aagacggtcg caaagtacaa tcgggttgta taggaaatat
     2401 tggttgtacg tctttctttc catcaaaaaa tttaggttgt tatggtgatg gaggtgcaat
     2461 ttttaccaat gatgacacct tagcagctgc tatgcgtgct attgctaacc atggtatggt
     2521 cgtacgttat taccacgata tgataggcgt taactctcgt ttagatagca tacaagctgc
     2581 tgtgcttgat gtaaaactta aaaaccttga tacctacata gcgaatcgtc aagctgctgc
     2641 aaaagcatac aacaaggcat ttgctaatca cccaaaaata actattcctt acgaatttcc
     2701 tcaatcaaca catgtgtatc accaatatac attaaaatta aaaaatgtag atagagctgc
     2761 tttacaaaaa gcattagaag caaaaggaat tccagctatg gtatattatc cagtgccatt
     2821 acatatgcaa aaagcatatc aagatg
//
LOCUS       DAOSMY010000211         2824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_35535_length_2824_cov_3.428313, whole genome shotgun sequence.
ACCESSION   DAOSMY010000211 DAOSMY010000000
VERSION     DAOSMY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35535_length_2824_cov_3.428313"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLD76_05230"
     CDS             <1..449
                     /locus_tag="PLD76_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOU27438.1"
                     /translation="RPTHQLLGVYNLKMIRLYNYINQIVGGSYALVHSLDGYDEVSLT
                     ADTKIVSSFGEELYSPEQMGFSTLKQEDLHAGNSIDEAAAIFKSVLENSSTKAQQDVV
                     VANSALAIRIVCPTKSIAECKQEALVSLQSGKAATAFKHFLESYKG"
     gene            454..1239
                     /gene="trpC"
                     /locus_tag="PLD76_05235"
     CDS             454..1239
                     /gene="trpC"
                     /locus_tag="PLD76_05235"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOU27439.1"
                     /translation="MTILDKIIAEKQHEVSVRKQEVSIHQLEKSSTFSRQTISLKKAL
                     QQSNTGIIAEFKRKSPSKGWIAEHAQASVIPFGYQQAGATGLSILTDYPFFGGQTKDI
                     LDAREQVTIPILRKDFMIDEYQLIEAKAMGADVILLIAAALSPKQVLTLAKMAHSLSL
                     EVLLEIHNEQELAHICEEVDMVGVNNRNLKDFIVDIQYSFDLVDKIPNECLKISESGL
                     QNPIDVQNLRKAGYQGFLMGETFMKTAHPEKALAAFIQQLRVN"
     gene            1263..1895
                     /locus_tag="PLD76_05240"
     CDS             1263..1895
                     /locus_tag="PLD76_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOU27440.1"
                     /translation="MKVKVCGMKYPQNIRDLEGLHPDYMGFIFYPPSPRYVGEDTELK
                     TLLPSISIKKVGVFVNASYDEIIKNRVDFSLDIVQLHGKEPEALCRQLKQQGIPVIKA
                     FSISSPADFLDTKAYVDVCDYFLFDTKTPQYGGSGLKFDWQILQAYTYHVPFFLSGGI
                     ALSDVEEIKKIAHPQLVAIDVNSRFETSPATKNINQLQQFIQEIKQLNTY"
     gene            1897..2673
                     /gene="trpA"
                     /locus_tag="PLD76_05245"
     CDS             1897..2673
                     /gene="trpA"
                     /locus_tag="PLD76_05245"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOU27441.1"
                     /translation="MDRIASLFATKKNRIISVYFTAGFPQLHDTVTIIKELAHQGVDM
                     IEIGIPYSDPMADGPVIQHSSQQALENGMSQKLLFEQLATIRQEVSIPLVFMGYLNTI
                     MQFGFERFCQQAAHIGIDGLIIPDIPLDVYVKEYKTIVEQYGLKFILLITPETTEERI
                     RQIDAVSSGFVYMVSSAATTGSQQSFDTEKQAYFKRIDAMKLKNPTVVGFGVSNNATF
                     EAACAYANGAIIGSEFIKQLQNQPSIPLAVSTLLSTIKGS"
     gene            complement(2674..>2824)
                     /locus_tag="PLD76_05250"
     CDS             complement(2674..>2824)
                     /locus_tag="PLD76_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOU27442.1"
                     /translation="PSHICLERFVSQTPTEYRIAPNWGIKNHEFIAKLDKRLQERNTF
                     QGRLC"
BASE COUNT          954 a          481 c          561 g          828 t
ORIGIN      
        1 ctcgacccac acaccaacta ttaggagtgt ataacctgaa aatgattcgg ttatacaatt
       61 atatcaatca aattgtagga ggttcgtatg ccttagtgca ttcgctcgat gggtacgatg
      121 aggtatcgct aacggctgat acgaaaattg ttagcagttt tggtgaggaa ttatattcgc
      181 cagagcaaat gggtttttct accttaaaac aagaagattt acatgcagga aattcgatag
      241 acgaggcagc agctatcttt aagtctgtat tagaaaattc aagtaccaaa gcccaacaag
      301 atgtggtggt agccaattcg gctttggcta ttcgtattgt gtgtccaact aagtcaatag
      361 cagaatgcaa acaagaggcg ttggtgtcgt tgcaaagtgg aaaagctgca actgcattta
      421 agcacttttt agaaagttat aaaggataaa gatatgacaa ttttagataa gattatagct
      481 gaaaaacagc acgaagtcag tgttagaaaa caagaggttt cgattcatca attagagaaa
      541 tcttctactt tttcacgaca aactatttcg ttaaaaaaag ccttacaaca gtcgaataca
      601 ggtattattg ccgaatttaa acgtaaatca ccttctaaag gctggatagc tgagcatgct
      661 caagccagtg ttataccctt tggttatcaa caagctggtg caacgggttt atcaatactt
      721 accgattatc ccttctttgg tggacaaacg aaagatattt tagacgcacg tgagcaagtt
      781 actattccta ttttgcgaaa agattttatg attgacgaat accaattaat tgaagctaaa
      841 gcgatgggtg cagatgttat tttgctcatt gctgctgcct tgtcgccaaa gcaagtgctg
      901 actttagcca agatggctca ttccttatcg ctagaggtat tgctcgaaat tcacaacgag
      961 caagaattag cccatatttg tgaggaagta gatatggtag gggtaaataa tcgtaatcta
     1021 aaagatttta ttgtcgatat acagtattct tttgatttgg tagataaaat accgaacgaa
     1081 tgtttgaaaa ttagcgaaag tggtttgcaa aatccaattg acgttcagaa tcttcggaaa
     1141 gctggttatc aaggattctt aatgggagaa acatttatga aaacagccca tcctgaaaaa
     1201 gcattggctg catttataca acaactcaga gtcaattagg tcaatttaaa taaaaaatgc
     1261 cgatgaaagt gaaggtatgt ggaatgaaat atccgcaaaa tattagagat ttggaaggct
     1321 tacatccgga ttatatgggt tttatatttt atcctccatc acctcgatat gtgggtgagg
     1381 atactgagtt aaaaacattg ttaccctcca tatcaattaa aaaagtggga gtttttgtca
     1441 atgcttccta cgatgaaata ataaaaaata gggttgattt ttcgttggat atagttcaac
     1501 tgcatggaaa agaacctgag gcattatgtc gtcaactgaa acaacaaggc atacctgtaa
     1561 ttaaggcatt tagtatttct tcgccagcag attttttaga taccaaagca tatgtggatg
     1621 tgtgtgatta ttttttattc gataccaaaa cacctcagta tggaggatcg ggactcaaat
     1681 ttgattggca aatattgcag gcatacacgt atcacgtacc tttcttttta agtggaggta
     1741 ttgctttaag cgatgtagaa gaaattaaaa agattgcaca tccgcaattg gtagcgatag
     1801 atgtaaacag tcgttttgaa acatctccag caaccaaaaa tatcaaccaa ttacaacaat
     1861 ttatacaaga aataaaacaa ctaaatacat actaacatgg ataggatagc atcattattt
     1921 gcaactaaaa aaaatcgaat aatatcagtg tattttacag caggatttcc tcaattacat
     1981 gataccgtaa ctattataaa ggagcttgca caccaaggcg tggatatgat agaaataggg
     2041 attccttatt ccgacccaat ggcagatggt ccagttatac aacatagtag tcagcaggca
     2101 cttgaaaacg gcatgtcgca aaaattgctt ttcgaacagt tagcaactat ccgtcaagaa
     2161 gtgtctatcc cgttggtatt tatgggctat ttaaatacta ttatgcaatt tggatttgaa
     2221 cgcttttgtc agcaagcagc acacattggc attgatggat taattattcc agatataccc
     2281 cttgacgtgt atgtaaaaga atacaaaacc atagtagagc aatatggatt aaaatttatt
     2341 ttattgatta cacccgaaac cacagaagaa cgtattcgac aaattgatgc cgtaagtagt
     2401 ggatttgttt atatggtatc ttcggcagct accacaggct cgcaacaatc atttgacaca
     2461 gaaaaacaag cttatttcaa acgcatagac gcaatgaaac taaaaaatcc aacagtagta
     2521 ggctttggcg tatccaataa tgctacgttt gaggctgctt gtgcgtatgc caacggagca
     2581 attataggca gtgaatttat aaagcaatta caaaatcaac catctattcc gttagctgtg
     2641 agtacattac tctcgacaat taaagggtcg taattaacac aaacgacctt gaaatgtgtt
     2701 gcgttcttgc aaacgtttat caagtttagc aataaactcg tggtttttta tgccccaatt
     2761 gggtgctata cgatattcgg taggcgtttg cgatacaaaa cgttcaaggc aaatatgcga
     2821 aggt
//
LOCUS       DAOSMY010000212         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_36014_length_2796_cov_3.526815, whole genome shotgun sequence.
ACCESSION   DAOSMY010000212 DAOSMY010000000
VERSION     DAOSMY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36014_length_2796_cov_3.526815"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLD76_05255"
     CDS             complement(<1..609)
                     /locus_tag="PLD76_05255"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOU27443.1"
                     /translation="MKTRQLILLALSLFLSQLSFASTFKVNGTILDSKKRGISNMVVS
                     TTQTTSGAITNNKGYFSIHAANPHDTLIINVLQDKILIPINEIKNIKITIADTITAEE
                     IIDETVNIGYQRIKKEDVTINPNTISGERLLATGETNLNEALLKVMPSIRQDRNGMLI
                     IRATQSLTQQGPPLYVVNGTPTNTVSNIPLSEIKDVQVLKDGS"
     gene            complement(623..1678)
                     /locus_tag="PLD76_05260"
     CDS             complement(623..1678)
                     /locus_tag="PLD76_05260"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU27444.1"
                     /translation="MIEELEEDLLELTDPSESPLFEHHRFVSDNGQGLVRIDKFLHDR
                     IVNSSRTKIQEAADAGNILVNGTAVKANYRVKPKDVITIVMAYPPREVEIIPQNIPLT
                     IVYEDDDLMVINKPAGMVVHPGHGNYDGTLVNALAYYFGNNANYNATDPRLGLVHRID
                     KNTSGLLLIAKNEYAKINLAKQFFDKTTERVYKALVWGNFQENEGTIKGNIGRSLKDR
                     MQMDVFPNGEQGKHAVTHYKVIENFNYVSFVECRLETGRTHQIRAHMKYIGHPLFNDE
                     RYGGNSILKGTTFNKYKQFVQNCFDTCPRQALHAQTLGFIHPISKEKLFFTSDIPEDM
                     KDLLEKWRNYTTSYVIN"
     gene            complement(1685..2494)
                     /locus_tag="PLD76_05265"
     CDS             complement(1685..2494)
                     /locus_tag="PLD76_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU27445.1"
                     /translation="MKRKPLNIFVKHLLIILGTFIAIGIVAFISLSIYTQHGKQETVP
                     NVKNMSITDAEKILNSNNLSYEIVDSIFNRALTPGFVAEQDPIPSSIVKKNRKIYLTI
                     NAKGVQLLPLPNGINMSLRQAKSLFEAADFVIKKIEYKNSEYKDLVLDVKLNNSSIPI
                     GKTIPAGSALTLVVGNGFNGELAIVPDVRNLSYEKATQLANESGFNLDEAIYDEVPLN
                     TADEKKYSIYRQEPRAKASLATGEFIKVWLSKTPQEYNTEEEISLEEDILF"
     gene            complement(2644..>2796)
                     /gene="rpmH"
                     /locus_tag="PLD76_05270"
     CDS             complement(2644..>2796)
                     /gene="rpmH"
                     /locus_tag="PLD76_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU27446.1"
                     /translation="RTFQPSNKKRKNKHGFRERMASANGRRVLASRRAKGRKKLTVSD
                     ELSLRK"
BASE COUNT          760 a          462 c          484 g         1090 t
ORIGIN      
        1 agaaccatct tttaatactt gaacatcttt tatttcactt aaaggaatat tacttacggt
       61 atttgttgga gtcccattca caacatacaa gggaggtcct tgttgagtta aagattgagt
      121 agcacgtata attaacattc cgttacgatc ttgtcttatt gaaggcatta cttttaggag
      181 ggcttcattt aagttggttt caccagttgc caaaagtctt tcgccagaga tggtattagg
      241 attaatagtt acatcttctt tttttattct ttgatagcca atatttacgg tttcatcgat
      301 gatttcttct gcagttatgg tatctgcaat ggtaatcttg atatttttaa tttcatttat
      361 agggatgagt attttatctt gtagtacgtt aattattaat gtgtcatgag gatttgctgc
      421 atgtatggag aaatagcctt tgttatttgt aattgcacct gaagtggttt gagttgtgct
      481 caccaccatg ttgcttatcc ctcttttttt gctatctaag atggttccat ttactttaaa
      541 agtagatgca aaacttaatt gagataagaa taagctaagt gctaatagta taagttgacg
      601 tgttttcatt gtgtatgttt ttttaattaa taacataact ggttgtgtaa tttcgccatt
      661 tctctagtaa atctttcatg tcttcaggaa tatctgacgt gaaaaaaagt ttctccttag
      721 atattggatg tataaatcct agtgtttggg catggagggc ttgtctcgga caggtatcaa
      781 aacaattttg tacaaattgt ttatatttat taaaggtagt tcctttcaga atactatttc
      841 caccataacg ttcgtcgtta aaaagaggat gtccgatata cttcatatga gcacgtattt
      901 ggtgtgttcg gccagtttct aaacggcatt ccacaaacga aacataattg aaattttcta
      961 taactttata atgagttaca gcgtgttttc cttgttctcc atttgggaat acatccattt
     1021 gcattctatc ttttaggctt cgaccaatat tgcctttgat tgttccttcg ttttcttgaa
     1081 aattacccca tactagggct ttgtaaactc gctctgttgt tttatcaaaa aattgtttgg
     1141 caagatttat ttttgcgtat tcgtttttag caattagcaa taacccagat gtattcttgt
     1201 ctatcctatg tactaaccct aatcgtgggt cagtagcatt gtaatttgca ttgtttccaa
     1261 aataatacgc tagtgcgttt actaaagtgc catcataatt tccgtgtcca ggatgtacta
     1321 ccattcctgc tggtttattt attaccatta aatcatcatc ttcgtatacg atagtaagcg
     1381 gaatattttg aggtataatt tccacctctc ttggaggata agccattaca atcgttatga
     1441 catctttggg tttaacccga taattggctt ttaccgcggt gccatttact aagatatttc
     1501 cagcatcggc tgcttcttgt attttggtac gagaactatt tacgattcta tcgtgtaaaa
     1561 acttgtctat acgaaccaat ccttgtccat tatcgctgac aaaacgatgg tgttcgaata
     1621 ggggtgattc tgatggatct gttaattcga gtaaatcttc ttctaattcc tctatcattc
     1681 tagcttaaaa tagtatatct tcttctagcg aaatttcttc ttctgtatta tattcttggg
     1741 gtgttttaga aagccaaact tttataaatt ctccagtagc taatgatgct tttgctctag
     1801 gttcttgacg atatatactg tatttttttt catcggcggt atttaatggt acctcgtcat
     1861 aaattgcttc atctaaatta aatccacttt cattagcaag ttgcgttgct ttttcgtagg
     1921 ataaatttct tacatctggt acaattgcta attctccatt aaaaccattt cctaccacaa
     1981 gtgtaagggc tgaacctgct ggaattgttt ttcctatagg aattgaactg ttatttagtt
     2041 ttacgtctag cacaaggtct ttgtattctg agtttttata ttcaattttt tttatcacaa
     2101 aatcagctgc ttcaaataag gattttgctt gtcttaacga catatttatt ccatttggta
     2161 agggtaataa ttgtactcct tttgcattga tagttaagta aatctttcta tttttcttta
     2221 caattgatga gggaataggg tcttgttcgg caacaaaacc aggtgtcagt gcacgattaa
     2281 atattgaatc aacaatttca tacgacaggt tgttggagtt tagtattttt tccgcatctg
     2341 taatagacat gttttttaca ttaggaactg tttcttgttt tccgtgctgt gtatatattg
     2401 ataagctaat gaaagcgacg ataccgattg caataaatgt tccaagtata ataagtaggt
     2461 gctttacaaa aatatttaga ggtttacgtt tcatgtctat attgtttcat tgttacacac
     2521 tattggcagt taattattca gtaattggat gctgcaaaga tacgcttttt tttgaataca
     2581 acgaaaagag agtaaaggct tgtgccttca ctctctcata ttcatagtac aagaaaataa
     2641 ttgttatttt ctcaaagaaa gttcgtcaga tacggttagt ttttttctgc ctttagctct
     2701 tcttgaagct aacactctac gaccattagc agaagccatt ctttcgcgaa aaccgtgttt
     2761 gttttttctt tttttgtttg atggttgaaa tgtcct
//
LOCUS       DAOSMY010000213         2753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_36777_length_2753_cov_3.272795, whole genome shotgun sequence.
ACCESSION   DAOSMY010000213 DAOSMY010000000
VERSION     DAOSMY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36777_length_2753_cov_3.272795"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLD76_05275"
     CDS             <1..1074
                     /locus_tag="PLD76_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOU27447.1"
                     /translation="PLGLNYGNLPKALLSFHRYAENTRTAFEEHLVEGVAYAKNVQNE
                     VAVHFTISPEHKSAFIEKMNHVLPIYEDAFSVTFKLSFSEQKPSTDTIAVNENNEIIR
                     NEDGSMLFRPGGHGALIENLNDCDADIIFIKNIDNVVVDTLKQSTYTYKKALAGLLLS
                     IQAATFDLLKKLDGNVDDATLKIIEDFAKNTLYIHVPSQYAVAPKEEKIAFWKKTLNR
                     PIRVCGMVRNEGEPGGGPFWVLNEQNEESLQVVESSQIDYKNKLQERIAVKASHFNPV
                     DIVCATKNMHGEKFYLPDFVDPKTGFISSKSKDGKTLKAQELPGLWNGAMANWISIFV
                     QVPISTFNPVKTVNDLLRKEHQA"
     gene            1195..2601
                     /gene="asnS"
                     /locus_tag="PLD76_05280"
     CDS             1195..2601
                     /gene="asnS"
                     /locus_tag="PLD76_05280"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU27448.1"
                     /translation="MEAIKRKKIVEALKSSSFGEKVNIKGWVRTKRGNKTVNFIAIND
                     GSTINNIQVVVDMEKFGEEILKPITTGACVSINGILTESLGKGQSVEIQATEIELYGA
                     ADPDTYPLQKKGHTLEFLREIAHLRPRTNTFGAIFRVRHQMAMAIHNFFHNKGFVYVH
                     TPIITGSDCEGAGQMFQVTTLNLYNLKKNEQGQIDYTDDFFGKQASLTVSGQLEGELA
                     AMALGEIYTFGPTFRAENSNTPRHLSEFWMIEPEMAFYEIADNMQLAEDFVKHCIQWA
                     LDNCQDDIQFLSEMYDKELVERLKFVVDNEFIRLTYTEGIEILEDAVKNGKKFEFPVG
                     WGTDLQSEHERYLVETHFKKPVILTDYPKEIKSFYMKQNADGKTVRAMDVLFPKIGEI
                     IGGSQREENYEKLRQRAAEMGVPEKDIWWYLDTRRFGTAPHSGFGLGFERLLLFVTGM
                     VNIRDVIPFPRTPNNAEF"
BASE COUNT          965 a          508 c          541 g          739 t
ORIGIN      
        1 cctctcggac ttaactatgg caatttgccc aaagccctac tatcgtttca tcggtatgcc
       61 gaaaacaccc gcacagcatt cgaagaacat ttggtagaag gagtggcgta tgcaaaaaat
      121 gtacagaatg aagttgctgt gcattttacc atctcgcccg aacacaaatc agcttttatc
      181 gaaaaaatga accacgtatt acccatatac gaggatgcat ttagtgttac attcaaacta
      241 tctttttctg agcaaaaacc atcgacggat accatagcag taaacgagaa caacgaaatt
      301 attcggaatg aagatggtag tatgcttttt agaccaggag gacacggagc gttgattgaa
      361 aacttaaacg attgtgatgc agatattatt ttcataaaaa atattgacaa tgtagtagtt
      421 gacaccttaa aacaatcgac ctacacctat aaaaaagcct tagcaggctt acttctgagc
      481 atacaagcag caactttcga tctattaaaa aaactcgatg gcaacgtaga tgatgccaca
      541 cttaagataa tagaagattt tgccaaaaac accctatata ttcacgtacc tagtcaatat
      601 gcagtagcac cgaaagagga aaaaattgct ttttggaaaa aaaccctaaa cagaccgata
      661 cgtgtgtgtg gtatggtaag aaatgaggga gaacctggtg gtggtccatt ttgggtgcta
      721 aacgaacaga atgaagaaag tttacaggta gtagaatcat cgcaaattga ctacaaaaac
      781 aagctacaag aacgcattgc agtaaaagca agccatttta accctgtgga tattgtctgt
      841 gccacaaaaa atatgcatgg agaaaaattt tatttacccg attttgttga ccccaaaact
      901 ggatttattt cctccaaatc gaaagacgga aaaaccttaa aagcacaaga attaccagga
      961 ttatggaacg gagcaatggc aaactggata agcatatttg tacaagtacc tatcagcaca
     1021 tttaatccag taaaaacggt aaatgacctg cttagaaaag aacaccaagc ctaaaaatag
     1081 acaaagtccc gcaaagggac ttttttatgt agaatttaac ggcaatttta ccacaattaa
     1141 ttgcaaaata cttacctttg tggcttaatt acgaaatttt atcatttaca gcagatggaa
     1201 gcaatcaaaa gaaaaaaaat tgtagaagcc ctaaaatcct catcatttgg tgagaaagtg
     1261 aatattaaag gctgggtaag aacgaaaaga ggaaataaaa ctgtaaattt tatcgccatt
     1321 aatgacggta gcactatcaa taatatacaa gtggtagtgg atatggaaaa gtttggagaa
     1381 gaaattctaa aacccattac aacaggagct tgtgtaagta ttaatggtat attaaccgaa
     1441 tcgttgggca agggacaatc cgttgaaata caagctactg aaatagaact ttacggagcg
     1501 gctgaccccg acacatatcc tttgcaaaag aaaggacata ccttggagtt tttgcgcgaa
     1561 atagctcatt tacgtccacg taccaatacg tttggagcca tttttagagt gcgccaccaa
     1621 atggcaatgg ctatccacaa cttttttcac aacaaaggat ttgtatacgt acatactccc
     1681 atcattactg ggtctgactg cgagggtgca ggacaaatgt ttcaggttac taccctcaac
     1741 ttatacaacc ttaaaaagaa cgaacaagga caaattgact atactgacga tttttttggc
     1801 aaacaagcca gtttaaccgt atcgggacag ctcgaaggcg aacttgctgc catggcattg
     1861 ggcgaaattt acacgtttgg acctactttt agggctgaaa attccaatac gcctcgtcac
     1921 ttatcagaat tttggatgat agagcctgaa atggcgtttt acgaaattgc tgacaatatg
     1981 caattagccg aagattttgt aaaacattgc atacaatggg ctttggataa ttgccaagac
     2041 gatatccaat ttttatcaga aatgtacgat aaagaactgg tagaacgttt gaaatttgtg
     2101 gtagataatg agtttatccg attgacctac accgaaggca tagaaatatt agaagatgcc
     2161 gttaaaaacg gtaaaaaatt cgagtttcct gtgggctggg gcaccgactt gcaatcagaa
     2221 cacgaacgct acttggtaga aacgcacttt aaaaaacccg ttatattgac cgactaccct
     2281 aaagaaatta agtcgtttta tatgaaacaa aatgccgatg gcaaaacggt acgtgctatg
     2341 gatgtgttat tcccaaaaat tggcgaaatc atcggaggtt ctcagcgtga agagaactac
     2401 gaaaagctac gccaacgtgc agcagaaatg ggtgtgcccg aaaaagatat ttggtggtat
     2461 ttggatactc gccgctttgg tacagctccc cactctggct ttggattagg ctttgaacga
     2521 ttactccttt tcgttactgg tatggtaaac atacgcgatg taataccatt tccacgcact
     2581 cctaataatg ctgagttttg atgaaaaaac tcttttgaat aaaataaagc tcctaaattt
     2641 gaaaaatatt ttaggggctt ttttatggat tagcccaata attgaaagaa aatattttta
     2701 cctaactaaa tattttttac agcagaaatt ttttgtacat ttgcacatat tgg
//
LOCUS       DAOSMY010000214         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_36851_length_2749_cov_2.811433, whole genome shotgun sequence.
ACCESSION   DAOSMY010000214 DAOSMY010000000
VERSION     DAOSMY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36851_length_2749_cov_2.811433"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLD76_05285"
     CDS             <1..454
                     /locus_tag="PLD76_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27449.1"
                     /translation="SASYKQQLIIRFVGKIAPEIRQKLSEKGIDDILEFVDYVPHNEA
                     LRYMKNAHLLLLLLPHASSNTGILTGKLFEYMASETPILMLASDTEEATQLLHSCQAG
                     RAVHPLDSVAIQLAIENAIDGKWGKASYKLYTDYSRRALTGRLASLIS"
     gene            complement(763..1698)
                     /locus_tag="PLD76_05290"
     CDS             complement(763..1698)
                     /locus_tag="PLD76_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27450.1"
                     /translation="MKIKEIKEIKYEKVAIIINCGTVLYSTLAILSIKKHSNLPIVLI
                     DCDTPNSGWDKKLSFKYFDNLMNHIDFDLLSLPLNIHGKTLDNIFREIQSKIVLLLDS
                     DAEILNSNIVDLFIKYSSQKQFWGSGFKTFFRKTTDFTYNILNGLYMERVFMPFCVLN
                     VEKVNEALCNNISFEAFIKNNDFPFNKKICKILRKRYKLPFFKKRPHIFTKYFTKGQK
                     FHDHYYPDWLYYDTGAKIYEYLCYNKEYWFCELSEDISTDYLLHAYGTTRGFLTNDKD
                     ANRQYQEKLLIDRMYNEYNFDIKNTNYYDIKKQSL"
BASE COUNT          901 a          446 c          401 g         1001 t
ORIGIN      
        1 gtcagcaagt tataagcagc aacttataat acgttttgtc ggtaaaattg ctcccgagat
       61 taggcaaaaa ttgagcgaaa agggaataga cgatatactt gaatttgttg attacgtacc
      121 ccataatgaa gcactacggt atatgaaaaa tgctcattta ttactgcttt tgttacctca
      181 tgcgtcttcg aatacaggta tccttactgg caaactcttt gagtatatgg caagcgaaac
      241 tcctattttg atgttagctt ctgatactga agaagccacc caactgctac atagttgtca
      301 agctggaaga gctgttcatc cattggacag tgttgctata caacttgcca tagagaatgc
      361 gatagatgga aaatggggta aagcaagtta taagctttac acagactact ctagacgagc
      421 attgacaggg agattggctt ctcttatttc atagctggtt tatatatttt aacaagcaga
      481 agatgtgaga aaacgtaatc ttaatatgtt tccctttgtt cccttattct aatataagca
      541 aaaatgttat aatatagtga ataatttatc ttgttttctt atgaaataag agtattacac
      601 tacttgcaag tttcgttgat attattctgt atataatgct ttgactattt tttcttctac
      661 tttgaagaga aaaagccaaa gcaaaatatg ataacattgt ttgtattcat ttattcaaat
      721 acaaacaatg aattttttta atttttaaat caatataact ttttataagg actgtttctt
      781 tatatcataa taatttgtat tttttatatc aaaattatat tcattataca ttcgatctat
      841 taacaatttc tcttgatatt gtctgttagc atccttgtca tttgtaagga atcctcttgt
      901 agtcccgtaa gcgtgtagta aataatctgt tgaaatatcc tctgataatt cacaaaacca
      961 gtattctttg ttataacaaa gatattcata tatttttgct ccagtatcat aatacaacca
     1021 atcaggataa tagtggtcat gaaatttttg tcctttagta aaatattttg taaatatgtg
     1081 tggacgtttt ttaaaaaatg gcagtttata cctttttcta agtattttgc aaattttttt
     1141 gttgaaagga aagtcattgt ttttaataaa tgcttcgaat gatatgttat tgcaaagagc
     1201 ttcattaacc ttctcaacgt tcaaaacaca aaaaggcata aaaacccttt ccatatataa
     1261 tccattcaaa atattgtagg taaaatcggt tgtttttctg aaaaatgttt tgaaaccaga
     1321 tccccagaac tgtttttgag aagaatattt aataaataaa tctacaatat ttgagtttag
     1381 tatctccgca tcactatcca atagtaaaac gattttactc tgtatttctc taaaaatatt
     1441 gtccagagtt ttcccgtgaa tattaagagg taaactcaat aaatcaaaat caatatgatt
     1501 catcaaatta tcaaaatatt taaaacttaa ctttttatcc caaccagaat taggagtatc
     1561 acaatctatt aaaacaatgg gtaaattgct atgttttttt atacttaata ttgctaatgt
     1621 gctatacaaa acggtgccgc agtttataat tattgcaact ttttcatatt ttatttcttt
     1681 tatttctttt attttcatat actttcttgt atataaccag accattcccc aagtttggtt
     1741 taaatactaa aaaaagcgta ggaattgtta tttaattcta tgttcgattc gtttcctaat
     1801 cttcgttctt gaaagtttaa atatagctat gactataata attcggaatt tttatttttt
     1861 tcttgcgttg agatgaggtt ggcgattttc agaaccaaga ataatttaaa acgctttatg
     1921 tttattgttt gaaaaatagg attatactaa atttttttct cttttttaag ccagagatta
     1981 ttttttgtaa catcatttat tcattcttgc cctctagttc ctcatatttg actccttttc
     2041 ccctctccgt tcctcaagtt cggtttatgt aaataaagaa agcgtgggaa ctgttaaatc
     2101 tatctgtttc cgaggtatcg ccaaacaccc acaacgcaga cagacaacag tcccacgccc
     2161 cttttagaag acgtgagcac tatcgctatc attcttgcgt tgttttgaaa ttggtgattt
     2221 ccgaaaacaa gaataatatc taaaacgctt tccgtaaaat taaaaataat cggttagctt
     2281 gctaactgat gcaaaaatat ataaattatt ttaattagtt tgtaataaac taaaaaaata
     2341 tatctaatct tttccttgtt tgtaaaaaaa aaactggcat ctcctagatt ctcttcacac
     2401 aaaaggtgta acatgtaaac ccaataacaa cctattttag atttgaacta gaaaactcct
     2461 gttcctttat ttattccgct ccgctggagc tacgttgtat tggtatgttt tttctattat
     2521 gatgtagccc ctacggggaa aattagctgt ttctgctaag aaattggcaa tggaaacagc
     2581 ccagctaaag aggagcgaca taataatatg tgccagtatt gttgttgaat gaaacaaagt
     2641 attaacaaaa cagccaacct agttaagact atcctgtagc caaaaaaaat cctatttttt
     2701 attccttcgc gtctctctgt cgactatttc ttacacgaag agtacgaag
//
LOCUS       DAOSMY010000215         2741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_36991_length_2741_cov_4.498883, whole genome shotgun sequence.
ACCESSION   DAOSMY010000215 DAOSMY010000000
VERSION     DAOSMY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36991_length_2741_cov_4.498883"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PLD76_05295"
     CDS             complement(<1..1163)
                     /locus_tag="PLD76_05295"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOU27451.1"
                     /translation="MSINKYNNIFFIGVAGVGMSAIAQYLQGIGKQVSGSDRYFVADQ
                     PNDTKNKLEAEGIHCFSQDGTGISENTDLVVVSTAIEDTVAEVQKAKQLNIPILKRSQ
                     VLALIAESKKTIAVGGTSGKSTVSAMIFDTLEFAGMEPSIISGAGLTSIIREGKIGNA
                     KVGKGEWLVIEADESDGSIVQYHPEIGLLLNIDKDHQEIDELMEIFGIFKQHTKSRFI
                     TNQSNKLAAQLSANLAHDFASSPSEKAGYVGSDFSQNGFEISFTINGKPFKMNAIGKH
                     SMENALATTAVCHQLGVSLETIAAALVQYEGIYRRHQVIGKKRGVTIIDDYAHNPAKC
                     AASISACQPISPKVIAWFQPHGYGPTRFLRADFVAEIAHVLRENDEIWMSEIF"
     gene            complement(1284..1360)
                     /locus_tag="PLD76_05300"
     tRNA            complement(1284..1360)
                     /locus_tag="PLD76_05300"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     assembly_gap    1321..1330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1425..2477)
                     /gene="mnmA"
                     /locus_tag="PLD76_05305"
     CDS             complement(1425..2477)
                     /gene="mnmA"
                     /locus_tag="PLD76_05305"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOU27452.1"
                     /translation="MYTKENVLLGMSGGTDSSVSAFLLQQAGYNVIGVTFRFYEHNDD
                     QTYLQKASNMAKHLHIPHYIVDKRKDFQSIVIENFTNEYLAGRTPFPCAHCNTYLKWD
                     ALLEQAHRLDCPHIATGHYVNTITKDNYTYLSMGKDIDKDQTFFLWGMPQEAIERAIF
                     PLGNLLYHEVQNIATSNQLPVKQNKNSVGACFCPTDYRPLLKKIATEKGIQIHQGIFT
                     DEIGNFLGKHEGYPFYTIGQRRGLGLQHISPLYVKKINRETNSIIVAPKESCYQTTVH
                     LTGLICRQLVEKEKQLTAKVQYKKQACTATLDMINTSEALVTLHEPTLGMAPGQSIVL
                     YDGDILIAGGIIQNAW"
     gene            2560..>2741
                     /locus_tag="PLD76_05310"
     CDS             2560..>2741
                     /locus_tag="PLD76_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27453.1"
                     /translation="MLVFMLLLLLYVPVYATESTCVSGTVSGYPGTTIELYYFTDEIS
                     YKQEVLTKTSIDSAGV"
BASE COUNT          748 a          535 c          535 g          913 t
ORIGIN      
        1 taaaagattt cgctcatcca tatttcatca ttttcacgta acacgtgggc tatttctgcc
       61 acaaaatcgg cacgcaaaaa tcgggttggt ccatatccat gtggctgaaa ccacgcaatt
      121 actttgggcg aaatgggctg acaagccgag atggaagccg cacatttggc tgggttgtga
      181 gcgtagtcgt caataatggt aacacctcgc ttttttccaa ttacttgatg tcgccgatag
      241 atgccttcat actgcaccaa cgcggctgca attgtttcta aacttacccc taattgatgg
      301 caaacagctg ttgtagccaa ggcgttttcc atgctatgtt tgcctatggc attcatttta
      361 aacggctttc cattgatagt aaaactgatt tcaaagccat tttgcgaaaa atcgctaccc
      421 acatagcctg ctttttcgct gggcgatgag gcaaaatcat gtgctaagtt tgccgacaac
      481 tgagcagcca atttgttcga ttgattcgta ataaaacgtg atttggtgtg ttgtttgaag
      541 atgccaaata tttccattaa ttcatcaatt tcctgatgat ctttgtctat attgagtaat
      601 agacctattt cggggtggta ttgtacgatg gaaccgtcac tttcgtcagc ttcaataacc
      661 agccattcgc cttttcctac tttggcgtta cctattttcc cttcacgaat aatactcgtt
      721 aagcctgcac cactaatgat gctaggttcc attcctgcaa attctaaggt gtcgaaaatc
      781 attgccgaaa ccgtactttt tcccgatgtg ccacctactg caatggtttt tttgctttcg
      841 gcaatcaagg ctagtacttg cgagcgtttg agtatgggga tgtttagttg tttcgctttt
      901 tgaacctcag caaccgtgtc ttcaatagcg gtagaaacca ctaccaaatc ggtattctcc
      961 gatattcccg ttccgtcttg cgaaaaacaa tgaattcctt cagcttctag tttgtttttt
     1021 gtgtcgttgg gttggtctgc cacaaagtac ctatcgctac cactcacttg tttgccaata
     1081 ccttgtagat attgtgcaat agcactcatg ccaacaccgg ctactccaat aaaaaaaatg
     1141 ttgttgtact tgtttataga catactcttt ttgttaaatg ctaatgattt ccaaaagtag
     1201 taatttttta cattacaaca atgtaaattc cactaaagta taggcaaaaa taaagcccaa
     1261 agttaacttt gggcttttgg ttgtggtacc accaggaatc gaaccgggga cacaaggatt
     1321 nnnnnnnnnn cctttgctct accaactgag ctatggtacc agttgcgggt gcaaaggtaa
     1381 tacctttttg ataaatacca aatattttta taaaaaaaat atgtttacca tgcgttttga
     1441 ataatcccac cagcaataag tatatctcca tcgtataaaa caatcgattg acctggagcc
     1501 attccgagtg taggttcgtg taaggtaact agggcttccg aggtatttat catgtctaat
     1561 gtagcagtgc atgcttgttt tttatactgc acttttgccg ttagctgttt ttctttttct
     1621 acaagttggc gacaaattaa tcctgttaaa tgtacggtcg tttggtaaca cgattctttt
     1681 ggtgctacaa taatggaatt ggtttctcta ttgatttttt ttacatacaa aggggagata
     1741 tgttgcaaac ccaaacccct acgttgacca atggtgtaaa atgggtaacc ctcgtgtttc
     1801 cctagaaaat taccgatttc atcggtaaat attccttgat gaatctgtat tcctttttct
     1861 gttgcaattt tttttaaaag aggtcggtag tccgtaggac aaaaacatgc acctacactg
     1921 tttttatttt gtttcaccgg aagttggttg gaggtagcaa tgttttgcac ttcgtgatag
     1981 agcaaattac ccaaagggaa aatagctcgt tcaatggctt cttgaggcat gccccaaaga
     2041 aaaaaagttt ggtctttatc aatatctttc cccatagaga ggtaggtgta attatccttt
     2101 gtgattgtat tcacataatg tccagtagca atatgggggc aatctaaccg atgagcttgt
     2161 tcgagtaagg catcccattt cagatatgta ttacaatggg cacaaggaaa aggggtacgc
     2221 cctgcaaggt attcattcgt gaaattttct attacaattg attggaagtc cttgcgttta
     2281 tcaacaatat agtgtggtat gtgtaggtgt tttgccatat tactagcctt ctgtaggtag
     2341 gtttggtcat cgttatgctc gtaaaaacgg aaagtaacgc caataacatt ataccctgct
     2401 tgttggagta aaaaggctga tacagaacta tccgttcctc cgctcattcc tagtagaaca
     2461 ttttcttttg tatacataaa catgttaact ttgtacaaaa gtacgttaaa ttattatttt
     2521 gtctatatct ttttgtattt ttacggaatg aaaaaaaata tgctggtttt tatgctcctt
     2581 ttgttgctat atgtgcctgt atatgcaact gaatctacgt gtgtgtctgg cacggtatct
     2641 ggatatccag gtactacgat agagttgtac tattttaccg atgaaatcag ctataaacaa
     2701 gaagttttaa cgaaaacaag cattgattct gcaggtgtat t
//
LOCUS       DAOSMY010000216         2737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_37069_length_2737_cov_3.762118, whole genome shotgun sequence.
ACCESSION   DAOSMY010000216 DAOSMY010000000
VERSION     DAOSMY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37069_length_2737_cov_3.762118"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /gene="recG"
                     /locus_tag="PLD76_05315"
     CDS             <1..1662
                     /gene="recG"
                     /locus_tag="PLD76_05315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU27454.1"
                     /translation="FQPLYHTTEKMKSKFVNSKAVQKLIYQALQTITPSLPESLPTSI
                     LHKYRFISLYEALINIHFPSNMPLLEQAQARLKFEELFYIQIAILRQAHALKTSFKGF
                     QFNYIGEMFHAYYEKCLPFELTNAQKRVLKEIRSNVRGAHQMNRLLQGDVGSGKTIVA
                     LMCMLMAIDNGYQACIMAPTEILARQHFETIESQINKLPIQVKLLTGSTKKKDREHIH
                     EGLLNGSVHILIGTHAVIEDTVNFKNLGIVVIDEQHRFGVAQRAKLWNKNTRPPHVLV
                     MTATPIPRTLAMTVYGDLEVSVIDELPPGRKPIVTNHIFEDTKREALNRFLKQQLSLG
                     RQVYIVYPLIEESEKMDLKNLTDGYQYIQSIFPDLQVSMVHGKMKPAEKDAAMQEFVQ
                     HKSQIMVATTVIEVGVNVPNASVMVIENAERFGLAQLHQLRGRVGRGADQSYCVLVTS
                     RKISNETRKRLEIMTRTNDGFEIAEADLQLRGPGDLEGTQQSGLPFQLHIANLAKDGH
                     FVQLARECASAILENDPQLKSPANEIIVKELKTKKREQQNWSIIS"
     gene            1744..2208
                     /gene="ndk"
                     /locus_tag="PLD76_05320"
     CDS             1744..2208
                     /gene="ndk"
                     /locus_tag="PLD76_05320"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU27455.1"
                     /translation="MMEKTLVIIKPSAISRGLIGEVISRFEKKGLHLIGMKMIELSDE
                     ILAEHYAHLAQKPFFQRIKNAMQTTPVIVCCWSGLDAVQVVRNMAGVTNGRNAASGTI
                     RGDYSMSVQENIVHTSDSVENAAIELNRFFKPEEIYNFPPHSLDFLYANDEL"
     gene            2219..>2737
                     /locus_tag="PLD76_05325"
     CDS             2219..>2737
                     /locus_tag="PLD76_05325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU27456.1"
                     /translation="MSYHFLRLIVFSFVVSITNSVLAVTLHDNIFRIEDPKNASSSLM
                     LLNENTEDDFADVDDFESFDEISFNAPYNSWSNTAVNPYNINLLHSKDTFRVDVSGYT
                     HPLDKVQRITSHFGPRRTRYHYGIDLKLNVGDTVRSSFDGMVRIAKIGRGYGYYVLVR
                     HFNGLETIYGHLS"
BASE COUNT          926 a          471 c          520 g          820 t
ORIGIN      
        1 tttcagccct tgtaccatac caccgaaaaa atgaaaagta agtttgtgaa ttctaaagcc
       61 gttcaaaaac tcatttacca agccttacaa accattacac catcattacc cgaatcttta
      121 ccaactagta tccttcacaa ataccgtttt atttcacttt acgaagcatt aatcaatatt
      181 catttcccca gcaatatgcc tcttttagaa caagcacaag cacgactaaa atttgaagaa
      241 ctattttata tacaaattgc aatattacgt caggctcatg ctcttaaaac tagttttaaa
      301 gggtttcaat ttaattacat tggagaaatg tttcatgcct attacgaaaa atgcttgccc
      361 tttgaactaa ccaatgctca aaaaagagta cttaaagaga tacgtagtaa tgtacgtgga
      421 gctcatcaaa tgaataggtt gttgcaaggc gatgtaggta gtggcaaaac tattgtagct
      481 ctaatgtgta tgcttatggc tattgataat ggttatcagg catgtattat ggctcctact
      541 gagattttag ccaggcaaca ttttgaaact attgaaagcc aaataaacaa attacccata
      601 caagtaaaat tacttacagg ttcaacgaaa aaaaaagaca gagaacatat tcacgagggc
      661 ttactgaatg gttctgtaca tattttaatt ggcacacatg ctgtaataga agatacagtt
      721 aattttaaga atttaggcat agtggtaatt gatgaacaac atcgatttgg tgtggcacaa
      781 cgagctaaat tgtggaataa aaacacacgt cctcctcatg ttttagtaat gaccgctact
      841 cccattccac gtaccttggc tatgactgta tatggcgatt tggaggtttc tgtaattgac
      901 gaattgcccc ctggacgaaa accaatagta acaaaccata tttttgaaga tacaaaacga
      961 gaggctctta atcgtttttt aaaacaacaa ctctcacttg gcaggcaagt atatattgtg
     1021 tatccattga tagaagaatc cgaaaagatg gatttaaaaa accttacaga tggctatcaa
     1081 tacatacaat caatttttcc agatttgcaa gtaagcatgg tacatggcaa aatgaaacca
     1141 gctgaaaaag atgctgctat gcaagagttt gtgcaacata aatctcaaat tatggtcgct
     1201 actactgtga ttgaagtggg ggtgaatgtg ccaaacgcca gtgtaatggt aatcgaaaat
     1261 gcagagcgat ttggattggc acagctccat cagctcagag ggcgggttgg tagaggagcc
     1321 gaccaatcgt attgtgtatt ggtaacgtcg cgaaaaatat ccaacgaaac ccgcaaacgc
     1381 ttagaaatca tgacccgtac caacgacggg tttgaaatag ccgaggctga cttacagctt
     1441 cgtggaccag gagatttgga aggtacccaa cagagtggct tgccttttca actacatatt
     1501 gccaatttag ctaaagatgg acattttgtt caactagcca gagagtgtgc ttcagcaatt
     1561 ctagaaaacg atccgcaact gaagtcccca gcaaacgaaa ttatagtaaa agaactcaaa
     1621 acaaagaaaa gagaacaaca aaattggagt ataattagtt gatgtgtaaa aattctataa
     1681 tttttttgca aaaaatttgg ttaactaaag aataaattgt ttttttgtac gttgtaaaat
     1741 caaattatgg aaaaaacact ggttattatt aaaccttcag ctatttcaag aggtttaatt
     1801 ggagaagtaa tatctcgctt cgaaaagaaa ggtttacacc ttattgggat gaaaatgata
     1861 gaattatctg acgaaattct tgccgagcat tatgctcatt tagcccaaaa accctttttc
     1921 caacgtatta aaaatgccat gcaaacaaca cccgttatcg tatgttgttg gtctggatta
     1981 gatgctgttc aggtggtaag aaatatggcg ggagttacca atggtcgtaa tgctgcttca
     2041 ggaaccatac gtggcgatta tagtatgagt gtacaagaaa acattgttca tacatccgac
     2101 tctgttgaaa atgctgcaat tgaactaaat cgctttttta agccagaaga aatatataat
     2161 ttcccaccac atagtttaga ctttttatat gccaatgacg aattgtaaaa ataaaaaaat
     2221 gagttatcat tttttacgtc ttattgtatt tagttttgtt gtttcaatca caaatagtgt
     2281 gttggctgta actttgcacg ataatatatt tcgcatagaa gacccgaaga atgcatctag
     2341 cagtcttatg ttgctaaacg aaaatacaga agatgatttt gctgatgtgg atgattttga
     2401 aagttttgat gaaatatctt ttaatgcacc ttacaacagc tggagcaata cggcggtcaa
     2461 tccgtataat ataaatttat tacatagcaa agatactttt cgtgtagatg ttagtgggta
     2521 tactcatcca cttgataagg tgcaacgcat aacatcacat tttggtccgc gtcgtactcg
     2581 ttatcactat ggtattgatt taaaattgaa tgttggagat actgtgcgtt cttcatttga
     2641 tggaatggta cgtatagcca aaataggtag agggtatgga tattacgttc tcgtgaggca
     2701 ttttaatgga ctcgaaacta tttatggtca tttaagc
//
LOCUS       DAOSMY010000217         2732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_37159_length_2732_cov_1.909227, whole genome shotgun sequence.
ACCESSION   DAOSMY010000217 DAOSMY010000000
VERSION     DAOSMY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37159_length_2732_cov_1.909227"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLD76_05330"
     CDS             <1..762
                     /locus_tag="PLD76_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU27457.1"
                     /translation="AGGGSGEAYDLDDYDFMDQPYKQLFVWDPDAEEIIGGYRFIHGS
                     QVFLKEDGQPNLVTSEMYHFSPQFIKEYLPYTIELGRSFVQPDYQSSKMGAKSLFALD
                     NLWDGIGALIVSVPDTKYFFGKVTVYPHYNAQARDLVFGFITKFFPDVDKLVYPIDPF
                     HIALSEEQINHVFVNNDYDEDYKILKKEVKDLGTSIPPLVNAYMSLSPAMRSFGFAVY
                     HDFGNLIEVCIMIPIEEMYDEKKKRHVESFFKELE"
     gene            934..>2732
                     /locus_tag="PLD76_05335"
     CDS             934..>2732
                     /locus_tag="PLD76_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27458.1"
                     /translation="MKQLHQHISSICARCLLSLCFVFLYINTISANFELYILDSSDNV
                     VTQSTFVNAGTNVADEMSTLLTIPSYTGNTADYQFYIADTNNLPDAKSDTVTLFSVMN
                     TCSSGEIKLLIYANSTQSNYEPHNAVCMDTQVYTYGMVCWDGLAANCDGTITSASSHV
                     FTGSAGSFLHTFTVNSVSGGFKLLKSPDGITWTWNDGQLGHVDSNTLLQYGTGVSVNL
                     GNGDIFLDIPGLVVGKTVSLQLDKTGSVYTLSATMLPDTPEVSNYSACQIAGTKTWAS
                     LVTATTGTLVWYADATSTTPIVAPTSFDTNDPQTTSYWVAQESGGIESNRVQVTASVF
                     AMPPANAAGEDRTICVGETTLLGVPSQPGFDYDWTPIAQLDNPSIAQPTTIALNSERT
                     FTLTVTNQQNTSCKATSSVTVSIRQRPVITMDKTEVSICAGTTTSFGNTNNLPNVSYE
                     WSPTVSLQDPFDGLFVTTQPLTSSTDFEHTVRWKDSPDCEATATVSVDVVANPIANAG
                     LDQNVCWGNVANIGSSLSTGQSFSWEPTGLIDGSTTFKTARTKAMEQTVEFTLTAKTS
                     FPLECTASDKVIVTVIPNPIAFSVTADKTAYCEG"
BASE COUNT          817 a          481 c          566 g          868 t
ORIGIN      
        1 gctggtggag gtagtggaga ggcttatgat ttggatgatt atgattttat ggatcaaccc
       61 tataagcaat tgtttgtgtg ggatccagac gcagaagaaa ttataggtgg ttatcgtttt
      121 atacatggtt ctcaagtgtt tttaaaagaa gacggtcaac ccaatttggt tacctccgaa
      181 atgtaccatt tttcacctca atttatcaaa gagtatttgc cctatactat cgaactgggt
      241 cgttcgtttg tgcagccaga ttaccaatcg agcaaaatgg gtgcaaaaag cttgtttgcg
      301 ttggataatt tgtgggatgg aatcggggct ttaattgttt ccgtgcccga tactaagtat
      361 ttctttggca aggttactgt gtatccgcat tacaatgcac aagcacgtga tttggttttt
      421 gggtttatca caaaattctt tccagatgtc gataaactag tgtatccaat tgatccgttt
      481 catattgctc tttctgagga acaaatcaac catgtatttg ttaacaatga ttatgatgag
      541 gattataaaa ttttaaagaa agaagtcaaa gatttaggca caagcattcc cccattggta
      601 aatgcgtata tgagcttgtc gccagctatg cgttcgtttg gttttgccgt ttaccatgat
      661 tttggcaatt tgatagaggt atgtataatg attcctatcg aagagatgta tgatgaaaag
      721 aaaaaaagac atgttgagtc tttttttaaa gaactggaat aatcaaagtt tgaaataatt
      781 agttaccaaa atagcgaaaa tgttacgttt cgctattttt tttgttaaat aatcacgtta
      841 aacataaaaa tatatgaaaa aattcagtta attatttata tatttgtagt ctctctttct
      901 ttagaagcga aaaaccttaa tattgaaact gctatgaaac agttacacca acatatttcc
      961 agtatctgtg caagatgctt gttgtcattg tgttttgtat ttttatatat caacaccatt
     1021 tcggcaaatt ttgaattgta tattcttgac agttctgata acgtagtaac ccaatcaact
     1081 tttgtaaatg caggcaccaa tgtggctgat gaaatgtcaa ctttgcttac tattccttcc
     1141 tacactggca atactgctga ttaccaattt tacatcgcag acaccaataa tcttcctgac
     1201 gcaaagtcag atacggttac tttgttttct gtaatgaata cctgttcatc tggtgaaata
     1261 aagctattga tttacgccaa tagtactcaa agcaactatg aaccacacaa tgcagtgtgt
     1321 atggatacac aggtttatac ctatggtatg gtgtgttggg atggtttggc tgcaaattgt
     1381 gatggtacaa ttacttcggc ttcttctcac gtatttactg gaagtgcggg tagttttttg
     1441 catacattta cggtgaactc tgtgtcaggt gggttcaaat tattgaagtc gcccgatggt
     1501 attacgtgga catggaatga tggtcagctt gggcatgtgg attcaaatac gctattgcag
     1561 tatggtacag gtgtttctgt aaatctaggt aacggagata tttttcttga tattccaggt
     1621 ttagtggttg gcaaaactgt ctctttgcaa ttggataaaa caggttctgt ttatacacta
     1681 tcagctacca tgttgcccga tactcctgag gtaagcaatt atagtgcttg tcaaatagca
     1741 ggtacgaaga catgggcaag tttggtaaca gctacaacag gcaccttggt gtggtatgct
     1801 gatgctactt ctactacacc cattgtagct cccacaagtt ttgacacaaa tgatcctcaa
     1861 accacctctt attgggtggc acaagaaagt ggaggaatag aaagtaacag ggtacaagta
     1921 acggcatctg tatttgcaat gcctccagca aatgcggcag gagaggatag aacgatttgt
     1981 gttggtgaaa ctactctatt gggtgttcca tcacagcctg gttttgatta tgattggacg
     2041 ccaatagccc aattagataa tccaagcata gcccagccaa ctaccattgc gttgaactca
     2101 gaaagaacat ttacattaac tgttaccaat caacaaaaca ctagttgtaa ggctacatcg
     2161 agtgttacgg tgtctatacg acaacgacca gtgataacga tggataaaac ggaggtaagt
     2221 atatgtgctg gtacaacaac ctcttttggt aataccaata acttaccaaa cgtaagttat
     2281 gagtggagcc caacagtatc attacaagat ccatttgatg ggctatttgt tactacccag
     2341 ccattaacat cttctactga ttttgagcat acagtacggt ggaaagattc ccctgattgc
     2401 gaggctactg cgactgttag tgtagatgtt gtggcaaatc caattgccaa tgcaggtcta
     2461 gatcaaaatg tatgttgggg taatgttgct aatattggta gctcactcag tactggacaa
     2521 tccttttctt gggagccaac gggtttgatt gacggtagta ccacttttaa aacagctcga
     2581 accaaagcaa tggaacaaac tgttgagttt acgttaactg caaaaacctc tttcccatta
     2641 gaatgtacag ccagcgataa agtaatagtt actgttatac caaacccaat agctttttca
     2701 gtaacagctg ataaaactgc atattgtgaa gg
//
LOCUS       DAOSMY010000218         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_37172_length_2731_cov_2.766816, whole genome shotgun sequence.
ACCESSION   DAOSMY010000218 DAOSMY010000000
VERSION     DAOSMY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37172_length_2731_cov_2.766816"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLD76_05340"
     CDS             complement(<1..793)
                     /locus_tag="PLD76_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU27459.1"
                     /translation="MAKQKKFLTCDGNQAASHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAANGRKNIFGEPVQVQEMQSEAGAAAAMHGALQAGALTTTFTASQGLLLMIPNMYKV
                     AGELLPGVYHVSARALASHALSIFGDHQDVMAVRQTGCALLATGSVQEVMDLAGVAHL
                     AAIKTRVPFVHFFDGFRTSHEIQKVEQLDQEDLIPLIDQEALQAFRKRALNPESPVLR
                     GTAQNPDIYFQAREACNPFYDAIPAVVEDYMAQISALTGRIYGLFD"
     gene            complement(859..2577)
                     /gene="rho"
                     /locus_tag="PLD76_05345"
     CDS             complement(859..2577)
                     /gene="rho"
                     /locus_tag="PLD76_05345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU27460.1"
                     /translation="MSTKNELEKKTLSELQRIAKELSVPKISSLEQQALVYAILDKEA
                     EKVAQKTVTKPTTVVARKEKRARVTKPVGKSNEKADDKSIETPVSVVELIPDVHKEDA
                     LKQQIVEVPKKKVITKKPTPPTLLKKTVESIQIDNQQAEEKVSVKSSISTVNTVVVDE
                     KQEENTGVDLPVTTQPNVVIKKEQRVITKPNHSNENSEKLYEFDNILKGTGVLEMVEN
                     YGFLRSAEYNYMPSPDDVYVSQSQIRLFGLKTGDFVDGYIRPPKEGEKYFPLVKVEKI
                     NGRDPAFVRDRVPFEHLTPLFPEEKFILNGRNSTLSTRIIDLFCPIGKGQRGLIVAQP
                     KTGKTVLLKEIANAILEHNPEVYMIILLIDERPEEVTDMARSVKAEVVSSTFDESAEK
                     HVKVASIVHEKAKRLVECGHDVVILLDSITRLARAYNTTAPASGKVLSGGVDANALHK
                     PKRFFGAARNIENGGSLTIIATALTETGSKMDDVIFEEFKGTGNMELQLDRRLSNKRI
                     FPAVDIMASSTRRDDLLLGTDILQRMWILRRHLSDMNSVEAMEFLKDRMEKTRDNEEF
                     LASMNG"
BASE COUNT          809 a          537 c          415 g          970 t
ORIGIN      
        1 aatcaaataa tccataaata cgtccagtta atgcggaaat ttgtgccata taatcttcta
       61 ccacagcagg aattgcatca taaaaaggat tacaagcctc acgtgcttga aaataaatat
      121 ctggattttg agcagtacca cgtaatactg gactttctgg atttaacgct cttttacgga
      181 atgcttgtaa tgcttcttga tcaattaaag gaattaaatc ttcttggtct aattgttcta
      241 ctttttgaat ttcatgtgat gttctaaatc catcaaaaaa atggacaaaa ggaacacgtg
      301 tttttattgc tgccaaatga gctactccag ccaaatccat aacctcttga acagatccag
      361 ttgcaagcaa agcacatcct gtttgacgaa cagccataac atcttgatgg tctccaaaaa
      421 ttgataaagc atgtgatgct agtgcacgag cagacacgtg atatactcct ggcaataatt
      481 caccagcaac tttatacata ttaggaatca tcaacaataa cccttgtgat gccgtaaatg
      541 tagtggttaa agcccctgct tgtaatgccc cgtgcatagc tgctgctgct ccagcttctg
      601 attgcatttc ttgtacttgt acaggttctc caaaaatatt tttgcgtcca tttgctgccc
      661 actcatctac atattctgcc atagttgagg atggcgtgat tgggtaaata gcagctactt
      721 cactaaacat atatgctata tgcgaagctg cttgattacc atcacatgtg agaaattttt
      781 tttgtttcgc cattttcgta atgattaatt attaattaag ttgtgtgttt ttttattcag
      841 actgacaaag atataaaatt atccattcat tgatgcaaga aattcttcat tatctcgtgt
      901 tttttccatt ctatctttca aaaattccat tgcctctacc gaattcatat cggataaatg
      961 tctacgcaat atccacattc gttgcaaaat atctgttccc aacaacaagt catccctcct
     1021 agtacttgat gccattatat ctactgcagg aaatattctt ttatttgata aacgcctatc
     1081 taactgtaac tccatgttac ctgtgccttt aaattcctca aaaattacat catccatttt
     1141 agaacctgtt tcagttaaag cagtagcaat aatggttaaa cttcctccat tttcaatatt
     1201 tcgagctgca ccaaaaaaac gtttgggctt atgcaaagca tttgcatcta cacctcctga
     1261 taatacttta ccagatgctg gtgctgtagt attatacgct cgagccaaac gggtaatcga
     1321 atctaacaaa ataacaacat catgaccaca ctcaacaagt cttttagctt tctcatgcac
     1381 aatactcgcc actttaacat gcttttcagc agactcatca aaggtagatg atacaacctc
     1441 tgcttttaca cttctagcca tatctgttac ttcttctgga cgctcgtcga tgagcaaaat
     1501 aatcatatac acttctggat tatgctctaa aatagcattc gctatttctt ttaaaagtac
     1561 agttttacca gttttaggtt gtgctacaat taagcctcgt tgtcctttcc caataggaca
     1621 gaataaatca attattcgag tagataaggt agaattacgt ccattaagta taaatttttc
     1681 ttctggaaac aagggtgtaa gatgttcaaa aggtactcta tctcttacaa atgcagggtc
     1741 cctaccattt attttttcaa ctttcacaag aggaaagtat ttttctcctt ccttaggagg
     1801 acgaatatat ccgtctacaa aatctccagt ttttaaacca aacaatcgaa tctgtgattg
     1861 cgacacatat acatcatcag gagaaggcat gtaattatat tcagctgaac gcaaaaaacc
     1921 ataattctct accatttcca atacgcctgt acctttcaat atattatcaa attcatacag
     1981 cttttcagaa ttttcattcg agtgattagg tttagtaata actctttgtt ctttttttat
     2041 tacaacattt ggttgtgtcg taacgggcag atcaacccca gtgttttctt cttgcttttc
     2101 gtcaacaaca actgtattta cagtacttat agaagattta acagagactt tttcttctgc
     2161 ctgttgatta tctatttgaa tagattctac tgtttttttc agtaaagtag gaggagttgg
     2221 ttttttagtg attacctttt ttttaggtac ttctactatt tgttgtttta atgcatcttc
     2281 tttatgtaca tctggtataa gttcaactac tgaaactggc gtttctatac ttttatcatc
     2341 tgctttttca ttactcttac ctacaggctt tgtaactcgt gccctttttt cttttcgagc
     2401 aactactgtt gtaggttttg ttactgtttt ttgtgccact ttctcagctt ctttatcaag
     2461 tatcgcatat actaaagctt gttgttctaa gctagatatt ttaggcacag ataactcttt
     2521 ggctatcctt tgaagttctg aaagagtttt tttctctaat tcattttttg ttgacatgta
     2581 aaaaggagtg ttttgtaacg taactaaata cttttgtcta taaacaaaag tctgtgtata
     2641 aaaatgtgta ggattgtttt aagtgcaaaa aaagagaact catttcacac cgtttgatgc
     2701 aacaaagata ctattatttt agtatataaa c
//
LOCUS       DAOSMY010000219         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_37369_length_2720_cov_3.938837, whole genome shotgun sequence.
ACCESSION   DAOSMY010000219 DAOSMY010000000
VERSION     DAOSMY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37369_length_2720_cov_3.938837"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLD76_05350"
     CDS             <1..501
                     /locus_tag="PLD76_05350"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOU27461.1"
                     /translation="VHIGWNENYIYLLFKVEEKHTLAQETALHGNVSQDSCVEFFVSF
                     DNRNTYYNFEFNAIGSMHVCCRNCDGTNKIAFTDKQLAAIETLTSFEKFTALNVYNTH
                     WQLGIAIPAPLFNKSDWYKNTIWANFYKCGDKLQEPHYLSWKSIVSPKPQFHVPHFFG
                     ALRCVK"
     gene            complement(515..1834)
                     /gene="rseP"
                     /locus_tag="PLD76_05355"
     CDS             complement(515..1834)
                     /gene="rseP"
                     /locus_tag="PLD76_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOU27462.1"
                     /translation="MDILIKVVQLLASLSILVLIHEMGHFLAAKLFKVRVEKFYLFFN
                     PWFTPIKFKPKNSETEYGIGWLPLGGYVKLAGMVDESMDTNQLKQPVQDWEFRSKPAW
                     QRLIIMAAGVIMNFVLAIAIYAATLFYYGEEYVPLHKVSLGMSFSEVAKKQGFQDGDI
                     LIAADGKTLKRLDEDNFRKILNAKQVLVNRAGDLTMVQLTEDFPQQLIASNEGFTSYR
                     IPFVVREVMPETPAFGAGLQKGDSVVSVNDDVLCTQDEFSNVFLANKNTQIMIGFYRQ
                     GDFLSTYITPDSAGMIGVYLTPFYEVYPTTKVTYGVLKSIPAGIKKGVKKFTGYASDM
                     KHLMSKQGAESIGGFGSITQLFPPFFSFEIFLEITAYLSVILAFMNILPIPALDGGHI
                     AFLTYEVITRRKVSQEVMMTAQKIGLLLLLFLLFYANGMDVVRAFFR"
     gene            complement(1841..>2720)
                     /gene="dxr"
                     /locus_tag="PLD76_05360"
     CDS             complement(1841..>2720)
                     /gene="dxr"
                     /locus_tag="PLD76_05360"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU27463.1"
                     /translation="PSVDVVLTAMVGYAGLLPTIHAVKAGKRIALANKETLVVAGEII
                     CDLAQQYRSAIIPVDSEHSAIFQCLIGEQNASVEKIILTASGGPFRQKTYDELRQVTT
                     RDALKHPNWEMGAKITIDSASLMNKGFEVIEAKWLFGLKPSQIEVLIHPQSIIHSMVQ
                     FVDGSVKAQIGLPDMKLPIQYAFAFPQRIKNNYPRLDFAQYARFDFQQPDTSKFRNLA
                     FAYDAMEKGGTMPCVLNAANEVVVAAFLEEKIGFLHMSDIIEKTMQTASWIAKPTYED
                     YVLADKQARELTLAQF"
BASE COUNT          859 a          600 c          461 g          800 t
ORIGIN      
        1 gtacatattg gttggaacga aaactatata tacctactgt ttaaggtaga agaaaaacac
       61 accttggcac aagaaaccgc actgcatgga aacgtatcgc aagatagttg tgtggaattt
      121 tttgtatctt tcgacaaccg taacacctac tacaattttg aattcaatgc cattggctct
      181 atgcatgtat gttgtcgcaa ctgcgatggc acaaacaaaa ttgcatttac tgacaaacaa
      241 ttagctgcaa ttgaaaccct gacgagtttt gaaaaattta cagctctcaa tgtgtataac
      301 acccattggc aattaggtat tgccatacct gctccattgt tcaataagag tgattggtat
      361 aaaaatacta tttgggcaaa tttttacaaa tgtggagata aattgcaaga acctcattac
      421 ctatcgtgga aatccatcgt aagcccaaaa ccacaatttc atgtacccca ctttttcgga
      481 gctttgcgtt gtgtgaaata agatttaagg cattttacct aaagaaagcc ctaaccacat
      541 ccattccatt ggcataaaac agcaagaata gcaacaacaa caagccaatt ttttgtgctg
      601 tcatcattac ctcttggctc actttacggc gtgttatcac ttcgtaagtt aagaatgcaa
      661 tatgcccacc atctaaagct ggtataggta atatattcat aaacgccaat ataacagaca
      721 gatatgccgt tatttccaaa aatatctcaa acgaaaaaaa cggagggaac aactgagtaa
      781 tggaaccaaa tccacctata ctttcagcac cttgcttgct cattagatgc ttcatatcgc
      841 tagcatatcc tgtaaatttc ttcacccctt ttttaattcc cgcagggata gacttcagca
      901 caccatacgt aaccttggta gttggataca cctcataaaa tggagtaaga tacaccccaa
      961 tcatgccagc agaatcaggt gtaatatacg tagagagaaa atcaccttga cgatagaacc
     1021 caatcataat ttgggtattt ttattggcta gaaatacgtt tgaaaactca tcttgtgtac
     1081 aaagcacatc atcattcact gacaccacac tatccccctt ttgcaaacca gcaccaaaag
     1141 cgggtgtttc gggcattact tctctcacca caaaaggtat acgatacgaa gtaaatcctt
     1201 cgttggaagc gataagttgt tgtggaaaat cttcggtcaa ttgcaccata gtcaaatcgc
     1261 cagccctatt taccaatact tgcttggcat tgagtatttt acggaaatta tcttcatcta
     1321 aacgttttaa tgttttgcca tcagccgcta tcaatatatc gccatcctga aaaccttgtt
     1381 ttttagcaac ttctgaaaac gacataccca aacttacctt atgcaaaggc acatattcct
     1441 ccccataata aaacaaggta gctgcataaa tagctatcgc taatacgaaa ttcatgataa
     1501 caccagccgc cataatgatt aaacgttgcc aagctggttt ggagcgaaac tcccaatctt
     1561 gcactggctg ttttaattga ttggtatcca tcgattcatc aaccatacca gctaatttta
     1621 catatccacc caagggcaac cagcctatac cgtattctgt ttcactattt ttgggtttaa
     1681 attttattgg agtaaaccac ggattgaaaa aaaggtaaaa cttttctacc cttactttaa
     1741 aaagcttggc agctaaaaaa tgccccattt cgtgtatcaa taccaaaatt gataaactcg
     1801 ccaataattg tactacctta attaatatat ccattgtttg ttaaaattgt gctaaggtta
     1861 actcccgagc ttgtttatcg gctaatacat aatcttcata agtcggtttt gcaatccaac
     1921 tagctgtttg catggttttt tctattatat cactcatgtg taaaaagcct attttttctt
     1981 ccaaaaatgc agctactacc acttcgttgg cagcattcaa tacgcaaggc atggttccgc
     2041 ctttttccat ggcatcataa gcaaaagcca aattacgaaa cttacttgtg tctggttgct
     2101 gaaaatcaaa acgtgcatat tgagcaaaat ccaaacgcgg atagttattt tttatgcgtt
     2161 gcggaaaagc aaaagcatac tgaataggta gtttcatatc tggcaaaccg atctgggctt
     2221 tcactgagcc atcaacaaac tgaaccatcg aatgaatgat ggactgcgga tgaatcaata
     2281 cctcaatctg cgaaggcttt aagccaaaca accatttagc ttctattacc tcaaatcctt
     2341 tgttcatcaa actggcagag tcgatggtaa tttttgcacc catctcccaa ttaggatgtt
     2401 tcaatgcatc acgggttgtt acttgacgca attcatcgta cgttttttgg cgaaaagggc
     2461 cacccgaagc ggtcagtatt attttttcga cagaagcatt ttgttcgcca atcaaacatt
     2521 gaaaaatggc tgagtgctcc gaatcgactg ggataattgc cgaacggtat tgttgtgcca
     2581 aatcgcagat aatctcgcca gctaccacca aggtttcttt gttggctaaa gctatacgtt
     2641 tgcccgcttt tacagcatga attgtaggca ataatcccgc gtatcctacc atagcagtaa
     2701 gtaccacatc aaccgacggt
//
LOCUS       DAOSMY010000220         2699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_37772_length_2699_cov_3.476551, whole genome shotgun sequence.
ACCESSION   DAOSMY010000220 DAOSMY010000000
VERSION     DAOSMY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37772_length_2699_cov_3.476551"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /locus_tag="PLD76_05365"
     CDS             complement(<1..2114)
                     /locus_tag="PLD76_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU27464.1"
                     /translation="MVVRGFVLNEQQQPIEMVDVSVQQSPTRTVTDKFGKFEIHVSAQ
                     DSFFLVFSSIAHRPAIRKVYVNEKYVTNVTVVLQDVINELTDIEVKGEKHQHTTAEWV
                     NTEFIKVLPSASGKSIESLIKSFSGVSSTNELSSQYSVRGGSYDENSVYVNGIEIYRP
                     FLIRSGEQEGLSFINTDMVGEVTFSAGGFDARYGDKLSSVLDITYKQPSTFEGNVAVS
                     FAGASAYIGSATNKWTQMHGLRYKNASYLLGTLQTKAEYNPNFIDYQSFVTYKPSTNW
                     SFSLLGNVSQNVYNFVPESRETTFGTLENLQKFTIYFDGQEKDIFRTGFGAFSTTYQP
                     NKKVGLTFIASAFATNEQENYDIKGEYWLNKVSSASSSSEEVMGVGTYFEHARNEMNV
                     FVCKATHHGTFDINRSHAIVWGVDAVSEKVIDAVNEWEMRDSAGYSLPYSPTEIRLYE
                     SLSSAHTFYSTRLRSYLQYSYKNISHHGLFVLTSGLRCNYWDWNNEMTISPRITASYF
                     PDWQQRINFRFATGVYYQTPSFKEIKDTITKNGNLTHFLNKNIQSQRSLQFIAGTDYF
                     FNWWYRPFKFTTEVYYKNMTNVISYTIDNVKVTYSGKNDAKAYAAGIDMKLFGEFVPG
                     VDSWLSVSIMNTKEDIDDDGYGYLARPMGQLYNFSIFFQDYVPKFPAYRVHLLFNWSE
                     GFPVTSARGERFYSNARMPDYR"
     gene            complement(2206..>2699)
                     /locus_tag="PLD76_05370"
     CDS             complement(2206..>2699)
                     /locus_tag="PLD76_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU27465.1"
                     /translation="LEEERRLFYVAITRAEKRCFISYSKSRFRNGQMTYAKHSRFISD
                     IDSHFLHLPVNFSSKNTSHFDFSQQKESFFSSNKPLTQTVIPKPTYNKNLTKLSAISN
                     KTSSQPVLSEVTIGSRIEHERFGKGVIIAITGDVADTRITVKFDNAGERVLLLKFAKF
                     SLC"
BASE COUNT          917 a          509 c          418 g          855 t
ORIGIN      
        1 ctacggtaat cgggcatacg agcattgcta taaaaacgct ctccgcgtgc tgaggtaaca
       61 ggaaaacctt ccgaccaatt gaataacaaa tgtactctat atgcaggaaa tttgggtaca
      121 taatcttgaa aaaagataga aaaattatat aattgtccca tcggtcgagc gagataacca
      181 tacccatcat cgtcaatatc ttcttttgta ttcataatag aaacactaag ccacgaatca
      241 accccaggca caaattctcc aaataatttc atatcaattc cagcagcata tgctttagca
      301 tcatttttac ccgaataggt aacttttaca ttatcaatag tatatgatat tacatttgtc
      361 atatttttat aatatacctc cgttgtaaat ttaaagggtc tgtaccacca attgaaaaaa
      421 taatcggtac ctgcaataaa ttgtaacgaa cgttgagatt gaatattttt atttaaaaaa
      481 tgagttaaat taccattttt cgtaattgta tctttaattt ctttaaaaga aggcgtttga
      541 taatacactc ctgtggcaaa tctaaaattt atccgttgtt gccaatcagg gaaatacgat
      601 gcggtgatac gagggctaat ggtcatttca ttattccaat cccaataatt acatcgcaaa
      661 cctgaggtta gcacaaataa accatgatga gaaatatttt tataactata ctgcaaataa
      721 gaacgtaatc tggtggaata aaaagtgtgt gcagaagata agctttcata tagccttatt
      781 tcagtggggc tatatggcaa tgaatacccc gcagaatccc tcatttccca ctcattaaca
      841 gcatctatta ctttttcgga tacagcatct actccccaca caatggcatg ggaacgattt
      901 atatcaaaag ttccatgatg agtagcctta caaacaaaca cattcatttc attacgtgca
      961 tgttcaaagt atgtacctac ccccattacc tcctccgaac tactactcgc agacgaaact
     1021 ttattaagcc aatactcgcc tttaatatca taattttctt gctcgttggt tgcaaatgct
     1081 gaagcaataa aagttaaccc gactttttta ttgggctgat aggttgttga aaacgctcca
     1141 aaacctgtac gaaaaatatc tttttcttgt ccatcaaaat agatggtaaa tttttgcaag
     1201 ttttctaagg tcccaaaagt tgtttctctt gattcgggta caaaattata cacattttgg
     1261 gatacattgc ctaatagaga gaaagaccaa ttggtactgg gtttatatgt aacaaaagac
     1321 tgataatcaa taaaattagg attatactct gctttagttt gcaacgttcc aagcaaataa
     1381 gaagcatttt tatagcgtaa gccatgcatt tgagtccatt tattagttgc tgaaccaata
     1441 taagcagatg ccccagcaaa gctgacagca acatttcctt caaaagtgga aggttgttta
     1501 tatgtaatat caagcacaga ggataattta tctccatatc tagcgtcaaa tccaccagct
     1561 gaaaaagtta cctccccaac catatccgtg ttgataaaac ttaacccttc ttgctctcct
     1621 gaacggataa gaaaaggacg gtaaatctct ataccattaa cataaacact attttcatca
     1681 taactaccac ctctaactga atactgagaa ctcagctcat tggttgaact cactccagaa
     1741 aatgatttaa tcaaactttc aatgcttttg cctgaggcag atggcaagac tttgataaat
     1801 tcagtattta cccattctgc tgtagtatgc tgatgctttt ctccttttac ttcaatatcc
     1861 gttaattcat ttattacatc ttgcaacact acagttacat tggtaacgta tttttcattt
     1921 acatatacct ttcgtatagc gggacgatgt gcaatggagg aaaaaactaa aaagaaagaa
     1981 tcttgggctg aaacatgaat ctcaaatttt ccaaatttat ctgtaacggt tcttgtaggc
     2041 gattgctgaa cagaaacatc aaccatttca ataggctgtt gctgttcatt cagtacaaat
     2101 cctctcacta ccatattttc atttgcaaat acatgtagta tgtttgtaca aatgataagc
     2161 aaaaagatga tatgtctaca cgaaattagc ataaataata aagaattagc ataatgaaaa
     2221 tttcgcaaat ttaagtaata aaaccctttc tccagcatta tcaaacttca cagttatacg
     2281 tgtatctgca acatctccag taatggctat tataacacct tttccaaatc gttcatgttc
     2341 gatacgtgag cctattgtaa cctcagataa aacaggttgt gatgaagttt tattgctgat
     2401 agcagacaac ttagttaagt ttttattgta tgtaggtttt ggtattacag tttgtgtcaa
     2461 tggcttatta gaactaaaga aagactcttt ttgttgcgaa aaatcaaaat gacttgtatt
     2521 cttagaagaa aagttaacgg gtaaatgcaa aaaatgagaa tctatatcag aaataaatcg
     2581 actatgttta gcataagtca tttgaccatt tctaaagcga ctttttgaat aagagataaa
     2641 acatcttttt tcggctctag taatagcaac gtaaaataaa cgcctctctt cttccaatc
//
LOCUS       DAOSMY010000221         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_38377_length_2665_cov_3.037548, whole genome shotgun sequence.
ACCESSION   DAOSMY010000221 DAOSMY010000000
VERSION     DAOSMY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38377_length_2665_cov_3.037548"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLD76_05375"
     CDS             <1..211
                     /locus_tag="PLD76_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOU27466.1"
                     /translation="CYEKSLLLKQGGYDYAYVTRARESEKGTMNTIEGDYWETENEYI
                     VYVYYRSFNDQSDRLIGKKIIRTTN"
     gene            224..643
                     /locus_tag="PLD76_05380"
     CDS             224..643
                     /locus_tag="PLD76_05380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOU27467.1"
                     /translation="MKQYDYELTFKVRDYECDLQGIVNNANYQHYLEHTRHEFLLSRG
                     ISFSELHEKGIDAVIARIEIAFKYPLRSKDEFVSKLNIRKEGIKNVFYQDIYRLPDMK
                     LCIRAKAENVCLVQGELKECPELEEKLNVFYAPQLSK"
     gene            733..1851
                     /locus_tag="PLD76_05385"
     CDS             733..1851
                     /locus_tag="PLD76_05385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27468.1"
                     /translation="MKIAIVSCFYPYRGGIAQFNANLYAVLSNEHNVKAFNFTRQYPS
                     LLFPVKTQYVTDKDTALAIESERVLDSANPFSYYSTAKKVLAYKPDVIILRYWHTYFA
                     PSMGYIARYATKRGYKVITIADNIVPHETHWFDTLFTKWFLKQNTALVTLSEAVRDDL
                     VRLHPAAKHMMKRHPLYNHFGEKISAEQARKQLHIDNNKKTLLFFGLIRDYKGLDLLL
                     DAMNLLDDSYQLVIAGECYGNFDTYQRQIDTNKNKQNIHLFHRYINDSEVPMFFSAAD
                     VCVLPYKTATQSGIAAISYHFEVPLVITNTGGLKEMVETPHTGLVCERIDSDSIAETI
                     QQFFTEDKTSFIEGIQAEKKALSWENFAQSLTEFIHNI"
     gene            complement(1852..2337)
                     /locus_tag="PLD76_05390"
     CDS             complement(1852..2337)
                     /locus_tag="PLD76_05390"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOU27469.1"
                     /translation="MEKIASFTIDHIRLLRGIYLSRTDLVQSETISTFDIRMKEPNRQ
                     PALSIASLHVIEHLAATFLRNHPIWAHKVIYWGPMGCCTGNYLILRGALQPIDIVELM
                     IETFQFIAHFEGDIPGMTPKDCGNCRLQDLPNAKNEAAIFVKEVLSVIQPENMIYPTE
                     K"
BASE COUNT          915 a          464 c          488 g          798 t
ORIGIN      
        1 atgctatgaa aaatcattac ttttaaaaca aggaggatat gattatgcct atgtaacacg
       61 tgctcgcgaa agcgaaaaag ggacaatgaa caccatagaa ggagactatt gggaaacaga
      121 aaatgaatat attgtgtatg tatattacag aagttttaac gaccaaagcg accgcctaat
      181 tgggaaaaaa ataattcgga caaccaatta acattacatt gatatgaaac aatatgatta
      241 tgaactaact ttcaaagtgc gagactatga atgtgatttg caagggatag taaataatgc
      301 taactaccaa cattatttag aacatacacg acatgaattt ttgctatcta gaggaataag
      361 tttttcagaa ttgcatgaaa aaggaattga tgcggttatt gcccgcattg aaattgcctt
      421 taagtaccca ctcagaagca aagatgaatt tgtaagcaaa cttaacatac gaaaagaagg
      481 aattaaaaat gtattttacc aagatattta tcgcttgcct gatatgaagc tgtgcattag
      541 agccaaagca gaaaatgtat gcttggtaca aggagagcta aaagaatgcc ccgaattaga
      601 agaaaaatta aacgtatttt acgctccaca acttagcaaa taaacagacc gtttgaacaa
      661 aaagacctta acacttcatc tttaatctga tattttttta ctacttttgt attctaattg
      721 tattctacga aaatgaagat tgccatcgta tcttgttttt acccgtatag gggaggtatt
      781 gcacaattca atgcaaattt atacgctgtt ttaagtaacg aacataacgt aaaggcattt
      841 aattttacaa gacaataccc atcattgttg tttcctgtaa aaacacaata tgtaaccgac
      901 aaggatactg ctttggcaat agaaagcgaa cgtgtactcg attcggcaaa cccattttcc
      961 tattattcta cggcaaaaaa agtgcttgct tataagcctg atgtaatcat attacgttat
     1021 tggcacacct attttgcccc ttcgatgggg tatattgctc gctatgctac caaacgtgga
     1081 tacaaagtaa tcactattgc cgacaatatt gtgcctcacg aaacacattg gtttgataca
     1141 ctttttacca aatggttttt gaaacaaaac acagcattgg taacacttag cgaagccgta
     1201 cgagacgatt tagttcgttt gcatcctgct gctaaacaca tgatgaaacg gcatccacta
     1261 tacaatcatt ttggtgaaaa aatatcggca gaacaagctc gaaaacagct acacattgat
     1321 aacaacaaaa aaaccttgct attttttgga ttaatacgcg actacaaagg cttagactta
     1381 ttgctcgacg ccatgaacct acttgatgac agttatcagt tagtgattgc aggcgagtgt
     1441 tatggaaatt ttgacacata ccaacgacaa atagacacga acaaaaacaa acaaaacata
     1501 cacctttttc atcgttatat caacgacagc gaagtgccta tgtttttttc ggctgctgat
     1561 gtatgcgtat tgccctacaa aacagctaca caaagtggca ttgctgccat ttcgtatcat
     1621 tttgaggtgc ccctagtaat taccaataca ggaggactaa aagaaatggt ggaaactccc
     1681 catactggac tagtgtgcga acgaattgat agtgatagta ttgcagaaac aatccaacaa
     1741 tttttcaccg aagataaaac gtcctttata gaaggtatac aagcagagaa aaaggctctt
     1801 tcgtgggaaa attttgcaca atcgcttacc gaatttatac acaatatcta gttacttttc
     1861 agtgggataa atcatatttt ctggttggat aactgataat acttctttta caaagattgc
     1921 ggcttcattt tttgcattgg gtaaatcttg caacctgcaa ttaccacaat ctttgggagt
     1981 catacctgga atatccccct caaaatgagc aataaattga aaagtttcta tcattaattc
     2041 aacaatatca atgggttgca atgctccacg aagtataaga taattgcctg tgcaacaacc
     2101 catcggtccc caataaatta ccttgtgtgc ccatatagga tgattgcgta aaaacgtagc
     2161 tgccaaatgc tctattacat gtagcgatgc aatagacaaa gcaggttgac gattaggttc
     2221 tttcatgcgt atatcaaagg tactgatggt ttcactttgt actaaatcag tacgcgaaag
     2281 gtaaatacct ctcaacagac gaatatgatc aatagtaaaa ctggctattt tttccatatg
     2341 tatatgataa atattgtttt aagatacaaa gatagaagta ttatttgttt tagcgactaa
     2401 aatataatta aaaaaaagga tagagttttt tcactttaga actgtatcca ccgattacaa
     2461 atcgacttcc gccattaggt tgatttttag cctacctaaa acgccctgaa aaggcaatgg
     2521 cgagctaagg gtaacagagg tgtttagtgt ttagtgttta gcgtttagtg tttagcgttt
     2581 agtgtttagc gtttagcgtt tagtgtttag tgtttagtgt ttagtgttta atgtttagtg
     2641 tttagtggat agtggatagt gataa
//
LOCUS       DAOSMY010000222         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_39088_length_2626_cov_2.808635, whole genome shotgun sequence.
ACCESSION   DAOSMY010000222 DAOSMY010000000
VERSION     DAOSMY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39088_length_2626_cov_2.808635"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLD76_05395"
     CDS             <1..498
                     /locus_tag="PLD76_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOU27470.1"
                     /translation="LRDVTQQYGATYTAAAVGEVNVVSKMKDTHAIIGGEGNGGVIYP
                     ESHYGRDALVGIALFLSHLAHKKMKVSALKATYPPYFISKQKVQLTPSIDVDAILNKV
                     KVAFSTEKITDIDGVKIDFPDKWVHLRKSNTEPIIRIYAEAATMEQADKLGAEIIAII
                     QQFAQ"
     gene            complement(563..1051)
                     /locus_tag="PLD76_05400"
     CDS             complement(563..1051)
                     /locus_tag="PLD76_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27471.1"
                     /translation="MKKLLLFILSSFMLVSCSLQSEADMSPQISFYDITINGESTTGQ
                     SGILVEDTLRMTVALQGYYHELTELVIVSEDDFVEFSFDESFQSSDWFASEITTSGTR
                     KFIFKSGIKTCVIPIQVVGIQKKQQAVELNFSLQSQSSAKGDYNPRKIDFAFTVSEQA
                     EE"
     gene            1184..1915
                     /locus_tag="PLD76_05405"
     CDS             1184..1915
                     /locus_tag="PLD76_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOU27472.1"
                     /translation="MIQEAITNYKLQHTITTLSPLKAILFDMDGVIFDSMPYHALSWV
                     NAFKQVGITFSEYEVYLNEGRTGASTIHNEFLSQKHRQASDAEIKELYALKSQHFEAC
                     GKPIAMNYATETLNRVKHLGLDIFLVTGSGQVSLLNTLNTYFPAVFSKQNMVTAYDVT
                     HGKPHPEPYLIALEKGNLQAQQAFVVENAPLGIQAAVAANIFTVAVNTGILEDKVLLE
                     AGANIVVPSLADFYHLLPSLVENWK"
     gene            complement(1917..2537)
                     /locus_tag="PLD76_05410"
     CDS             complement(1917..2537)
                     /locus_tag="PLD76_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU27473.1"
                     /translation="MKKFLFILSLLLFTSALYAQNIKLPTPNKSGGKPLMTVLNERKS
                     SRDFSEKILPNQQLSNLLWAAWGYNRADKRTAPSSQNKQEMDIYVALASGCYLYDARK
                     NELVLVVKQDLREKTGKQDFVKNAPVNIIFVADKRKMAQQDEQSMLQTAYINTGFISQ
                     NIYLYCASEGLATVIRAWVDKAALALEMKLHDQQEIIVCQTVGYPK"
BASE COUNT          869 a          484 c          425 g          848 t
ORIGIN      
        1 ctacgtgatg ttacccaaca atatggagct acctataccg ctgcagctgt tggtgaagta
       61 aatgtggtaa gcaaaatgaa agacacacat gccattattg gaggcgaagg taatggtgga
      121 gttatctacc ctgagagcca ctatggaaga gatgctttag tgggaatagc tctgtttctt
      181 agtcatttag ctcataaaaa aatgaaagta tctgcactaa aagcaactta ccctccttat
      241 tttatttcca aacaaaaagt tcaactgaca ccatcaattg atgttgatgc tattttaaac
      301 aaagtgaaag tagcattttc tacagaaaaa attactgata ttgatggagt aaaaattgat
      361 tttccagata aatgggttca tttgcgtaaa tcaaatacag aacccatcat tcgcatttat
      421 gccgaagcag ccactatgga acaggctgac aaattgggag ctgaaattat agccattata
      481 caacaatttg cccaataaaa tacctaaaaa taaatttagt cttatacaat caaatctaca
      541 ttgaggctat ataagactaa atttattcct ctgcttgttc agacacagta aaagcaaaat
      601 caatttttcg tggattataa tctccctttg ccgaagattg tgattgtaat gagaaattta
      661 attccacagc ttgttgtttc ttttgtatcc cgactacctg aatgggtatc acgcatgttt
      721 taatgcccga tttaaaaata aactttcttg ttcctgaagt agttatttca gaagcaaacc
      781 aatcacttga ttgaaatgat tcgtcaaacg aaaattctac aaaatcatct tctgacacaa
      841 ttactaactc cgtaagttcg tgataataac cttgcaatgc aacagtcatt cgcaaagtat
      901 cttctactaa aataccgctc tgtccagtag tcgattctcc attgatagtt atatcataaa
      961 aactaatttg tggactcatg tctgcttctg actgtaagct acacgacacc agcataaaac
     1021 tagacagaat aaataaaagt aattttttca tgtttattta actttaggta tgttagaatg
     1081 cctgcaaata tacagaaggc gaataacata tacgtacttt tttatttaaa tagtgtaaaa
     1141 aggtaggtaa aattgtgtaa ttttgtgtgc aaataaaaaa tacatgatac aagaagctat
     1201 taccaactat aaactacaac acactattac tacgcttagt ccactcaaag ccattttgtt
     1261 tgatatggac ggagtaattt ttgattcaat gccctatcat gccctttcgt gggtaaatgc
     1321 atttaaacaa gttggtatca ctttttctga atacgaagta tatctcaacg aggggcgaac
     1381 aggggcttct accattcata atgaatttct ttctcaaaaa cacagacaag cttctgatgc
     1441 tgaaataaaa gaattatatg ccttgaaaag tcaacatttt gaagcatgtg gtaagccaat
     1501 tgcgatgaac tatgctaccg aaactctcaa tcgagttaaa catcttggat tagatatatt
     1561 tttggttaca ggttctggtc aagtaagttt attaaacacc ctaaatacct atttcccagc
     1621 agtattttca aagcaaaata tggttacagc gtacgatgta acacatggca aaccacatcc
     1681 tgaaccctat ctcatagctt tggaaaaagg aaacttgcaa gctcaacaag cttttgtagt
     1741 agaaaatgcc ccattaggca ttcaagctgc tgtggcggct aacatattta cggttgccgt
     1801 taatactggc attttggaag ataaagtgtt gttagaagca ggtgctaata ttgtcgttcc
     1861 tagtttagca gatttttatc acttactacc ctcgcttgta gaaaactgga aataaatcac
     1921 tttggatatc ctactgtttg acaaacaatg atttcttgct gatcgtgcaa tttcatttct
     1981 aaagccaatg ctgctttatc gacccatgcc ctgatgacag ttgctaatcc ttcggaggca
     2041 caatataagt aaatattttg gctaataaaa ccagtattaa tgtatgctgt ttgcaacata
     2101 ctttgctcat cttgttgagc cattttcctt ttatcggcta caaaaatgat atttacagga
     2161 gcatttttca caaaatcttg tttgcctgtt ttttcgcgta aatcttgttt aacaactagt
     2221 actaattcgt ttttacgtgc atcatacaaa taacagccac ttgctaaagc cacgtaaata
     2281 tccatttctt gcttgttttg agaagaaggt gctgtacgtt tatctgccct attataaccc
     2341 caagccgccc ataataaatt ggataactgc tgattaggca aaattttttc tgaaaaatca
     2401 cggctacttt tacgttcgtt taaaactgtc attaagggct taccaccact tttatttgga
     2461 gttggcaact tgatattttg tgcatataag gcactggtaa ataaaagtaa tgacaatata
     2521 aacagaaatt ttttcataca actttgtatt ttataaaaac acctacaaat ataagaaaac
     2581 tacataactt ttgtctcttt atgctctctt tctatatcca caatgt
//
LOCUS       DAOSMY010000223         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_39131_length_2623_cov_4.165498, whole genome shotgun sequence.
ACCESSION   DAOSMY010000223 DAOSMY010000000
VERSION     DAOSMY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39131_length_2623_cov_4.165498"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /gene="pheT"
                     /locus_tag="PLD76_05415"
     CDS             <1..1482
                     /gene="pheT"
                     /locus_tag="PLD76_05415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU27474.1"
                     /translation="GIFGGLDSGVSNTTTKLFLESAYFNPVSIRKTARRHGLNTDSSF
                     RFERGCDPNNTLYALKRAALLIKEVAGGEISSDIIDIYPSPIQATEVKLTYAKINSLI
                     GKVIDKEIIRTILTGLEIDIVNENNDVLTLSVPAYRVDVQRDVDVIEDILRVYGYNNI
                     IPSDELKSSITYSQKPDSHKLQNTLAEQLTGAGFNEILNNSLTKIAYYKELNSFPENE
                     AVKILNPLSADLSVLRQTLLFGGLESITYNSNRRNPNLKFYEFGNCYHYDDAKKNNEE
                     PLKAYSEELHLGLWITGNKTKQSWTDTERKVSFYDLKGYVETILQRLGIKNESISVSD
                     YSNDLLPNALEIVSKNKMPLVVIGKVSNKVLKQFDIDTDVFYADFNWENLLKVIVKQK
                     VRYQEIPKFQEVKRDLALVLDKHIRFQDIEQIAFATEKNLLKKVTLFDVYEGKNIEQG
                     KKSYAISFVLQHDQKTLEDSQIETIMKKILQALETQTGAKLRQ"
     gene            1510..2223
                     /locus_tag="PLD76_05420"
     CDS             1510..2223
                     /locus_tag="PLD76_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU27475.1"
                     /translation="MGRAFEYRKATKLKRWGNMARVFTKLGKQITIAVKEGGPDPDTN
                     PRLRVLAQQAKKENMPKENVERAIKKASSKDEADYKEMVYEGYGPNGIAIVVETATDN
                     PTRTVANIRSYFNKFGGSLGTSGSLQFLFDHKCVFKIAPKPTVSLEDLELELIDFGVD
                     ELLEDEGEIILYGEFQSYSSIQKYLEENGFEIHSAEFERIPNDMKTLSEEQKAQVNKL
                     LDKIEDDDDVQNVYHNMEE"
     gene            2281..>2623
                     /locus_tag="PLD76_05425"
     CDS             2281..>2623
                     /locus_tag="PLD76_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOU27476.1"
                     /translation="MQNRPQTPHEERLNGISHLVGIIWSICIIPYCMYIAYDANDLMP
                     FLSVAIFAIGMLAVYTSSTLYHITTDPKRKMILKKFDHISIYFLIAGTYAPFIAHFVP
                     KNTAIIFLIIMW"
BASE COUNT          992 a          415 c          458 g          758 t
ORIGIN      
        1 ggtattttcg gaggattaga ctctggagta agcaatacta caactaaatt atttttagaa
       61 agtgcttatt ttaatccagt aagtatacgc aaaacagctc gtagacatgg actcaacaca
      121 gactcttctt ttcgttttga aagaggttgc gacccaaaca acaccttata tgcactaaaa
      181 cgtgccgcat tactcattaa agaagtagct ggtggcgaaa tttcttctga tattattgat
      241 atatatccct ctcctattca agcgacagaa gtaaagctta catacgccaa aattaattca
      301 ttaataggaa aagtaattga caaagaaata attagaacaa tattaactgg cttagaaata
      361 gacatagtta acgagaataa tgatgtactt acgctgtctg ttcctgccta cagagtagat
      421 gtacaacgtg atgtagatgt gattgaagat attttgcgtg tatatggtta caacaatatt
      481 attccgtctg acgaattaaa atcatccatt acgtattctc aaaaacctga ttcacataaa
      541 ttacaaaaca cactagcaga acagcttaca ggagctggtt ttaacgagat tttaaataat
      601 tcgcttacaa aaattgctta ttacaaagaa ctaaactctt ttccagaaaa tgaagcagtg
      661 aaaatactaa atcctttaag tgctgattta agtgttttgc gacaaacctt gctgtttggt
      721 ggtttagaaa gcattacgta taacagtaac agaagaaatc ctaatttgaa attttacgaa
      781 tttggaaact gctaccacta cgatgatgct aaaaaaaata acgaagaacc cctaaaagca
      841 tattcagaag aattgcattt gggcttatgg ataacaggaa acaaaacgaa acaaagttgg
      901 acagatactg aacgtaaagt gagtttttat gatttaaaag ggtatgtaga aacaatattg
      961 caacgattag gcattaaaaa tgaatctatt agcgtttctg attacagcaa tgacctattg
     1021 cctaatgctc tcgaaattgt aagtaaaaat aaaatgccat tggtggtaat cggaaaggtt
     1081 tctaataaag tgttaaaaca atttgacatt gacacagatg tcttttatgc agactttaat
     1141 tgggaaaatc ttctgaaagt tattgtaaaa caaaaagttc gttaccaaga aatacctaag
     1201 tttcaagaag tgaaacgaga ccttgcatta gtactcgaca aacatattcg ttttcaagat
     1261 attgagcaaa tagcatttgc tacagagaag aatttgttga agaaagtgac tttgtttgat
     1321 gtatatgaag gaaaaaacat tgaacaaggg aaaaaatcat atgccatcag ttttgtgtta
     1381 caacacgacc aaaaaacatt agaagattct caaatagaaa ctattatgaa aaaaattctt
     1441 caagccttag aaacacaaac tggagcaaaa ttacgtcaat aattaacctt atataacaaa
     1501 ttacaactca tgggaagagc atttgaatat agaaaagcta caaagctgaa acgttggggc
     1561 aatatggctc gcgttttcac aaaattaggt aaacaaatta cgattgcagt aaaagaaggc
     1621 ggtcctgacc cagatacaaa tccacgtctg cgtgtgctag cccaacaagc taaaaaagaa
     1681 aatatgccca aagaaaacgt agaaagggct ataaaaaaag catcttccaa ggatgaagct
     1741 gattacaaag aaatggtata tgaaggctat ggaccaaatg gtatagctat tgtggtagaa
     1801 acagcaacag acaacccaac tcgcaccgtt gcgaacatac gtagttactt caataaattt
     1861 ggaggttcgc taggaactag tgggtcttta caatttttat ttgatcataa atgtgtgttt
     1921 aaaattgctc caaaaccaac agtctcatta gaagatttgg aattagaatt aatagatttt
     1981 ggtgtagatg agctactaga agacgaagga gaaattattt tatacggcga atttcaatcg
     2041 tattctagca tacaaaaata cctcgaagaa aatggatttg aaattcacag tgctgaattt
     2101 gaacgcatac ccaacgatat gaaaacactt tcagaagaac aaaaagctca ggtaaacaaa
     2161 ctacttgata aaattgaaga tgatgatgat gtacaaaatg tatatcataa tatggaggaa
     2221 taagctaatc aaatagtagg gctaagctaa gtaagtttag cctttttttc ttatattact
     2281 atgcaaaata gaccacaaac accacacgaa gaacgattga atggaataag ccatttggtt
     2341 ggtattattt ggagtatatg cataattcca tattgcatgt atattgccta cgatgccaat
     2401 gatttaatgc cattcctttc tgttgcaatt tttgcaattg gaatgttagc ggtatacact
     2461 tcatctacgc tttaccatat taccacagac cctaaacgaa aaatgatact taaaaaattt
     2521 gaccatataa gtatttattt tttaattgct gggacatacg ccccattcat agcacatttt
     2581 gtaccaaaaa atacagcaat tatttttttg attattatgt ggt
//
LOCUS       DAOSMY010000224         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_39264_length_2617_cov_2.679157, whole genome shotgun sequence.
ACCESSION   DAOSMY010000224 DAOSMY010000000
VERSION     DAOSMY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39264_length_2617_cov_2.679157"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLD76_05430"
     CDS             complement(<1..722)
                     /locus_tag="PLD76_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU27477.1"
                     /translation="MDILLGSIQIPIQKIFSVILTNHHDGYSEIIWQFRLPKALTATL
                     VGSSLAVSGLLMQTLFKNPLADPYVLGISSGASLGVALLIMASGASWIPIFMVTSGWG
                     QIFAAIIGALLILMLVLLFSTRINDTVSLLIVGMMFGSITGALVNVLQSITNPDALKI
                     FVIWTMGSLSAVSWEYMYIMFPMLFAGLLISLLLPKQLNALLLGENYAKSIGVTVFRL
                     RFIIILVTCLLAGAATAFTGPIA"
     gene            complement(794..1936)
                     /locus_tag="PLD76_05435"
     CDS             complement(794..1936)
                     /locus_tag="PLD76_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU27478.1"
                     /translation="MRNIFFYTLIFFVICCTATGCTKQQKQDSLDSTTPIRSVPISYA
                     TNFSIDHFETYTRVIVYNPWQQNTILQTYYLVENISTTTPDNGIKIQIPIHTLVVNSC
                     SHIEFICMLNELESIVGISSPELVFNPIIRQRVANGKIENMGDAFAMNIEKILIKKPN
                     AIMVTGYNQMSDSHLRLETTHIPIIVNIEWMEASSLGRAEWIKFIAAFYKQEEIADSL
                     FQQVEKNYKELLALAQTVPSKPSIASGNSFKGTWYLPGGNSFMGKLYYDAGGDYVFAK
                     DSSSGSLPLSFEVAMKDLRHCDIWLGSNANSLHELVQMDSRLMLFDAYKNKQVYNYSK
                     RTTPSGGNDYWESAIARPDILLKDLIKVLHPNLLPDYELFYINRLE"
     gene            2231..2464
                     /gene="rpmB"
                     /locus_tag="PLD76_05440"
     CDS             2231..2464
                     /gene="rpmB"
                     /locus_tag="PLD76_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU27479.1"
                     /translation="MSKICQITGKSAMVGNNVSHSKRRTKRTFDINLFTKKFYWVEQD
                     AWISLKISANGLRVINKLGLNAAIKRAAEKGYL"
     gene            2486..>2617
                     /gene="rpmG"
                     /locus_tag="PLD76_05445"
     CDS             2486..>2617
                     /gene="rpmG"
                     /locus_tag="PLD76_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU27480.1"
                     /translation="MAKKAKGNRIQVILECTEHKTSGMPGTSRYISTKNRKNTTERLE
                     "
BASE COUNT          922 a          458 c          410 g          827 t
ORIGIN      
        1 gctataggac cagtaaaagc ggttgcagct cctgccagta aacaggtaac taatatgatg
       61 ataaaccgca atctaaaaac agtaactcca atactttttg cataattctc tccaagtaat
      121 aatgcgttta attgttttgg taatagtaaa gaaataagaa gcccagcaaa taacattgga
      181 aacataatat acatatactc ccaacttaca gcacttaaac tgcccattgt ccaaatcaca
      241 aatattttta gagcatctgg gtttgtaata ctttgcaata catttaccaa tgcacctgta
      301 atactaccaa acatcatccc tacaattaat aatgaaacgg tatcgttgat gcgtgttgaa
      361 aataacagta ccagcattaa aataagtaaa gctcctatta tagctgcaaa aatctgcccc
      421 caaccactcg ttaccataaa aattggtatc cacgatgcac ctgaagccat aatcaataaa
      481 gccactccca aacttgctcc agagcttata cccaacacat agggatctgc taaaggattt
      541 ttaaacagag tttgcattaa taatccagaa acagctaaag acgaccctac taacgtagcc
      601 gtcagggctt tcggtaatcg aaactgccaa atgatttcgc tgtatccatc gtgatgattg
      661 gtcaagatta cagaaaatat tttttgtata ggtatctgaa tacttcccaa aagtatatcc
      721 ataagaaaaa caaataccaa tatgataagt aatagcaaaa atataattgt tgtacgaagt
      781 ggcatgaatg tgtttattct aatcggttta tataaaataa ttcataatca ggtaataaat
      841 taggatgaag aacttttatt aaatctttta ataaaatatc tggacgtgca attgctgatt
      901 cccaataatc atttccacca ctaggtgtag tacgtttgct ataattatat acttgtttgt
      961 ttttataggc atcaaatagc atgagcctgc tatccatttg aactaattca tgcaatgagt
     1021 ttgcgtttga ccctaaccat atatcacaat gtcgcaaatc tttcattgcc acttcaaagc
     1081 tcaaaggtaa actacccgaa ctcgaatctt ttgcaaacac ataatcccct ccagcatcat
     1141 aatataattt acccataaaa ctattgcccc caggtaaata ccatgtacct ttaaaactat
     1201 tgccagatgc tatgctaggc ttagaaggta cagtttgtgc cagggctaac aattctttat
     1261 agtttttttc tacttgttga aagaggctgt ctgctatttc ttcttgtttg taaaatgccg
     1321 ctataaattt aatccactct gccctgccta aactggaggc ttccatccat tcaatattta
     1381 caatgattgg aatatgtgta gtttctaatc gtaggtgtga atcactcatc tgattataac
     1441 cagttaccat aatagcatta ggctttttta tcaaaatttt ttctatgttc atcgcaaacg
     1501 catcacccat attttctatt ttaccatttg ctacacgttg tctaataata ggattaaata
     1561 caagttcagg agagctaatt cctacaatgc tttcaagttc gtttagcata cagataaatt
     1621 caatatgact acacgagttg acaacaaggg tatgtattgg aatttgtatt ttaattccat
     1681 tgtcaggagt tgtagttgat atattttcta ccaaataata tgtttgtaag atggtgttct
     1741 gttgccaagg attatacaca attacgcgtg tataggtttc aaaatgatca atagaaaaat
     1801 ttgttgcata ggaaatagga acagaacgaa tgggtgtagt gctatctagg gagtcttgtt
     1861 tctgttgttt tgtgcaaccc gtcgcagtac aacaaattac gaaaaaaata agtgtataaa
     1921 aaaaaatatt cctcatcaat gcataaatta gcatacaaag ttacactttt ttatcaaatc
     1981 ttatttttac aactgtattt ttcttgtttt tacgtctggt atatctattt aaatgtcata
     2041 aaaatttggt tatatagata aaaaatgcga aatttgcacc cgcttataag aacaaaacac
     2101 gcttataaga cttgagtatt gaatcattca ggctttttgt tgataaattc ttgaatgttt
     2161 cttttgttat gtgcaaaaaa agtattttct ttgcactcta tttttaaaaa caacaattaa
     2221 ggatacagcg atgtcaaaaa tttgtcaaat tacaggaaaa agtgccatgg ttggcaacaa
     2281 tgtttctcac tcaaagagaa gaacaaaaag aacattcgat attaaccttt ttacaaaaaa
     2341 gttttattgg gttgaacaag atgcgtggat tagccttaaa atttcagcta acggacttcg
     2401 tgtaataaac aagctaggtt taaatgctgc aattaaaaga gctgctgaaa aaggttattt
     2461 ataataaata tagggagata cgaaaatggc aaaaaaagca aaaggaaata gaatacaagt
     2521 tattcttgaa tgcaccgagc acaaaactag tggtatgccc ggaacttctc gttacatttc
     2581 aactaagaac aggaaaaata caactgaaag attagaa
//
LOCUS       DAOSMY010000225         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_39275_length_2616_cov_3.974229, whole genome shotgun sequence.
ACCESSION   DAOSMY010000225 DAOSMY010000000
VERSION     DAOSMY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39275_length_2616_cov_3.974229"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLD76_05450"
     CDS             complement(<1..1182)
                     /locus_tag="PLD76_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOU27481.1"
                     /translation="MNKIVKRILISFLVLFILVFATLLTAPYFFKDKILTLAKQEVNA
                     LLNARADFSGIKLSFIRNFPNATVQLQDFSIVGVADFEADTLFYAKDFGMVVNLKSLF
                     ADNGYDVKRIEVDEASVKLRILPDGRANWDIMKEDTLAVEDTSAFAFHFKLQDFTIKR
                     TNFSYIDEEGDMQLYLTNINHHTTGDLTADSSLLVTKTTAETVTFVMEGMEYLSKASA
                     VFDATINANLNDMKFTFSENQSQLNAVPFAFDGWLKSIDNGWAMDITLLASEVDFKSL
                     LSLVPAVYATDFASLQADGKVSLTGFVKGDMVGDYYPAFGLQLAVENAWFKYPDLPKK
                     LDQITIKTAITNPGKTLDETVIDVSRFAFRLGNNPFAASMHITTPMSDPNVDFKANGK
                     LN"
     gene            1422..1727
                     /locus_tag="PLD76_05455"
     CDS             1422..1727
                     /locus_tag="PLD76_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOU27482.1"
                     /translation="MTGTKKISYIEQQQRTIKGMIAYYCKKKHQQGQLCEECKTLTAY
                     ALARLSACKYGENKPSCRKCPTHCYAPNKRKMMEERMRVVGPKMVYLRYFLSAYENY"
     gene            1749..2291
                     /locus_tag="PLD76_05460"
     CDS             1749..2291
                     /locus_tag="PLD76_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOU27483.1"
                     /translation="MTNYMNPWHHVSPHTTQTDSVQAIIEIPKGSRAKYELDKESGLI
                     KLDRVLYSSVYYPVNYGFIPQSYCNDKDPLDILVLSMIELTPMCIVTAKVIGVMQMVD
                     SGATDNKIIAVATGDPSVSHYNDIEELPPHFISELRNFFEDYKKLEKKHVEVDEFLNK
                     KAALEIVAESFVYYDTTFRK"
     gene            2294..>2616
                     /locus_tag="PLD76_05465"
     CDS             2294..>2616
                     /locus_tag="PLD76_05465"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOU27484.1"
                     /translation="MQKEGQDIQVFWFRRDLRLTDNVGLYNALQDKRYPVSPLFIFDT
                     DILSSLPSTYDKRVDYIHQILTSLNQELHQLGSSLMVEIGKPTEVFEKLTTQFTIKAV
                     HTNSD"
BASE COUNT          906 a          506 c          451 g          753 t
ORIGIN      
        1 attcagtttg ccattggctt taaaatccac atttgggtcc gacataggtg tagtgatatg
       61 catagaggca gcaaaaggat tgtttcccaa acggaaagca aaacgtgaca catcaattac
      121 tgtttcatcc aaggtttttc cggggttggt aatggctgtt ttaatggtta tttgatctaa
      181 tttttttggt aaatcaggat acttaaacca cgcattttct accgctaact gcaaaccaaa
      241 tgcaggataa taatccccta ccatatcgcc ttttacaaaa ccagttaacg acacctttcc
      301 gtcagcttgc aagctggcaa aatcagtagc atatacagca ggtacaagcg ataacaaaga
      361 cttaaaatcg acctcacttg ccaacaacgt aatatccata gcccacccat tatcaatgct
      421 tttgagccaa ccgtcaaatg caaagggaac agcatttaat tgcgactgat tttcgctaaa
      481 tgtaaacttc atatcattca aattggcatt gatagtagca tcgaaaacgg cactagcttt
      541 ggatagatac tccataccct ccatcacgaa tgttaccgtt tcggcagttg ttttggttac
      601 aagaagtgaa ctatcggcag ttaaatcgcc cgtagtatgg tggttgatat tggtaagata
      661 caattgcata tcgccttctt cgtcaatata ggaaaaattg gtgcgtttaa ttgtaaaatc
      721 ttgcaactta aaatgaaaag caaatgctga ggtatcttct actgccaagg tatcttcttt
      781 cattatatcc caattggctc tcccatcagg caaaatacgc aacttaacgg atgcttcgtc
      841 aacttctatg cgttttacat cgtaaccatt atcggcaaat aaacttttca ggtttacaac
      901 catgccaaaa tctttggcat aaaacaaggt atctgcttca aaatcggcta caccaacgat
      961 actaaaatct tgcaactgaa cggttgcatt tggaaaattg cgaataaaac tcaattttat
     1021 acccgaaaaa tcggctcgag cgttcaacag tgcattgact tcttgttttg ctaaggtcaa
     1081 aatcttatct ttgaaaaagt aaggagccgt aagcaaggtg gcaaaaacaa gaataaacaa
     1141 cactaaaaaa ctgataagta ttctttttac aattttattc atagtatagc aacattaaaa
     1201 aaattataga aatcagaaga atggaattta aactacaaaa ataataaaaa agaataccta
     1261 tacctgttta tcccagtttt ttttacttct tttatgattc cagaaaaaat ttgccctaca
     1321 cacacgaaat taagtacatc ttcatagtgc cattttgtat aattatacct atcttagcga
     1381 tgaaattgta aaatgctaac tccatttgta ttgttacgat aatgacagga acaaaaaaaa
     1441 tatcctatat tgaacaacaa cagcgaacaa taaaaggcat gatagcttat tattgcaaaa
     1501 aaaagcacca acaagggcaa ctttgcgagg aatgcaaaac attaacagct tatgcgttag
     1561 ctcgtttaag tgcctgcaaa tacggcgaaa acaaacctag ttgccgcaaa tgcccaactc
     1621 attgttatgc tcccaataaa cgcaaaatga tggaagaacg catgcgtgta gtaggtccaa
     1681 agatggtata tttacgctac ttcctttctg cttatgaaaa ttattaaatc aataaaaaaa
     1741 gtatctatat gactaattac atgaatcctt ggcaccacgt atcgcctcat actacgcaaa
     1801 cggatagtgt gcaagcaatt atcgaaatac ccaaaggaag tagggcaaaa tacgaactcg
     1861 acaaagaaag tggcttaatt aaactcgatc gagtgttgta ctcatcagtg tattatcctg
     1921 taaactacgg atttattccg caaagttatt gcaacgacaa agacccattg gatattttgg
     1981 tgctatcaat gattgaactt acccccatgt gtatagtaac tgccaaagtt attggagtaa
     2041 tgcaaatggt agatagcggt gcaactgaca ataaaataat agcagtagca actggcgacc
     2101 caagtgtgag tcattacaac gatatagagg aactgccacc tcattttatt tcggaattaa
     2161 gaaacttttt cgaagactat aaaaaactgg aaaagaaaca cgtagaggta gatgaatttt
     2221 tgaacaaaaa agctgctttg gagattgttg ccgagagttt tgtatattac gatactacgt
     2281 ttagaaaata agcatgcaaa aagagggaca agacatacag gttttttggt ttcgtcgcga
     2341 cctacgtttg actgacaatg ttggtctata caatgccttg caagacaaac gatatcctgt
     2401 atcccctcta tttattttcg atacagacat tttatcttcc ttgccttcta cgtatgataa
     2461 gcgggtagat tatattcatc aaatccttac atctcttaac caagaattgc atcaattagg
     2521 ctcatcgttg atggtagaaa ttggcaaacc aaccgaggtt ttcgaaaaat tgaccactca
     2581 atttacgatt aaagcagtac atacaaactc cgatta
//
LOCUS       DAOSMY010000226         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_39332_length_2613_cov_3.917123, whole genome shotgun sequence.
ACCESSION   DAOSMY010000226 DAOSMY010000000
VERSION     DAOSMY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39332_length_2613_cov_3.917123"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLD76_05470"
     CDS             complement(<1..542)
                     /locus_tag="PLD76_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU27485.1"
                     /translation="MNNEKATFLQAYQRLRRAVQPFAKKEDIHQIKQLYIHFFPSLGE
                     KEYRLMTKNFHVAAIAVEEIGLGRSAVMAILLYRLVVEEKITIQEIEKSYSKDIFIIV
                     KGLIQVNDLYTKTTSLETENFRRLLLTFAEDIRVIFIKLASRLYVMRELKSYEVDVQE
                     EIARETAFLYAPMAHRLGLYS"
     gene            729..1997
                     /locus_tag="PLD76_05475"
     CDS             729..1997
                     /locus_tag="PLD76_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOU27486.1"
                     /translation="MLPPLAERMRPQHLEEYIGQQHLVGEGAILRKMIESGSITSFIL
                     WGPPGVGKTTLARIIASKMNRGFYALSAVNSSVKDIREIIEKAKKTQFFDTPSPILFI
                     DEIHRFSKSQQDSLLAAVENGTVMLIGATTENPSFEVITPLLSRCQVYVLKHLEEQDL
                     RSLLNRVLEKDAVCKSKKIVVQETDALFRFAGGDARKLLNIIELLIGTENQTEIVLTN
                     KLVTEKLQENLAIYDKGGEMHYDIISAFIKSIRGSDPDAAIYWLARMIDGGEDPKFIA
                     RRLLISATEDIGLANPNALLLANTCFDAVHKIGFPESRIILAETTIYLASSKKSNSAY
                     LAIDKALDIVRKTGNLSVPLHLRNAPTSLMKNLEYGKNYVYPHDYPQNFTPQTYLPQE
                     LEKQSIWESQSNSTEKNLSEWLRSLWKNRF"
     gene            complement(1994..>2613)
                     /locus_tag="PLD76_05480"
     CDS             complement(1994..>2613)
                     /locus_tag="PLD76_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU27487.1"
                     /translation="NSLSRLKTSYIDILLLHRPDALVEPEEVAEAFETLFQQGKVLHF
                     GVSNHNSHQLALLQKHIQHKLLINQLQFSIMHSGIIDAGFNVNMNTEQAIDKNGGVLD
                     YCRLNDITIQAWSPFTYGFFEGTFIDNERFWQLNQTLHEISTKYNVSKTAIATAWILR
                     HPAKMQVVAGTTKPNRLQEICEGTTVTLTREEWYQIYLAAGNTLP"
BASE COUNT          812 a          439 c          494 g          868 t
ORIGIN      
        1 gagtataaac ctaatcgatg agccattggt gcatataaaa aagctgtttc acgagcaatt
       61 tcttcttgca cgtctacttc atacgatttt aattcacgca tgacatataa tctacttgcc
      121 aatttaatga aaattacccg tatatcttcg gcaaatgtga gtagcaagcg tctgaaattt
      181 tcggtttcga gcgaagttgt tttcgtgtat aagtcgttaa cttgaattaa tcctttcaca
      241 ataataaaaa tatctttgct gtaggatttt tcaatttctt gtatggtaat tttttcttct
      301 actaccaatc ggtatagtag aatagccatt acagccgaac gaccaagacc gatttcttca
      361 acagcgatag ccgcaacatg aaaatttttt gtcattaagc ggtattcttt ttctcctaag
      421 gagggaaaaa agtgtatata taattgcttg atttggtgta tatcttcctt tttagcaaaa
      481 ggttgaacag ccctgcgtaa tcgttgatag gcttgtaaaa aggtagcttt ttcgttgttc
      541 attgttttgc ttttagttaa tgtcgtaaaa atacaaaaaa tatagtgcaa ctagtgtctt
      601 tttttatttt tttattactt gtaattcaaa aaaatctact tgttgttaca agcatatttt
      661 accttatttt tgtctattaa actaaaaaat aaatgtacct ttgtcatcat acatacaagc
      721 acatagccat gttaccacca ttagcagagc ggatgcgtcc gcaacattta gaagaatata
      781 ttggacaaca acatttagtt ggtgaaggtg ctattttgcg taaaatgata gaatcgggta
      841 gtataacgtc ttttatttta tggggtcctc caggtgttgg taaaacaacc ttggcacgta
      901 tcattgcttc aaaaatgaat agaggttttt atgccttgag tgctgtaaat tcgagtgtaa
      961 aagatatacg agaaataatt gaaaaagcaa aaaaaacgca attttttgat acacctagtc
     1021 ctattctttt tattgacgag attcatcgtt ttagtaaatc acaacaggat tcgttattgg
     1081 ctgccgtaga aaatggtaca gttatgctta tcggagccac aacagaaaat ccatcatttg
     1141 aagtaattac tcctttatta tctaggtgtc aggtatatgt gttaaaacac ttagaagagc
     1201 aagatttacg ctctttgctc aatcgagtat tagaaaaaga tgcagtgtgt aaaagcaaga
     1261 aaattgttgt tcaagaaacc gatgctttgt tccgttttgc gggtggagat gcacgtaagt
     1321 tgctcaatat tattgaactc ctaattggta cagaaaacca aacagagata gttcttacga
     1381 ataaactcgt aaccgaaaaa ttacaagaaa acttggctat ttacgacaaa ggcggcgaaa
     1441 tgcattacga tattatctct gcgtttatta aatcgattag aggaagcgat cctgatgcag
     1501 caatttattg gttagctcgt atgatagatg ggggagaaga ccctaagttt atagctcgca
     1561 ggttgcttat ttcagctaca gaagatattg gattagctaa tccgaacgca ttgttactag
     1621 caaatacttg ttttgatgct gtacataaaa tcggttttcc cgaatcacgt attatattag
     1681 ctgaaacaac gatatatttg gcgagtagta aaaaaagcaa ttcagcctat ttggcaattg
     1741 ataaagccct tgatattgtt cgtaaaacag gaaatctttc cgttccattg catttgcgta
     1801 atgctcctac atccttgatg aaaaacttag agtatggcaa aaattacgtg tatccgcacg
     1861 attatccaca gaatttcacc ccacaaacgt acttgccaca agaacttgaa aagcaatcaa
     1921 tatgggaaag tcaatcgaat agtacggaaa aaaacctgtc ggagtggttg cgttcgttgt
     1981 ggaaaaatcg tttttaaggc aatgtatttc cagcagctag atagatttga taccattcct
     2041 cacgcgtaag tgttaccgtt gtgccttcac aaatttcttg taacctattt ggtttggtgg
     2101 tgccagccac aacttgcatt ttagctgggt gtcggagtat ccatgctgtt gcaatggcag
     2161 ttttagatac attgtatttg gtgctaattt cgtgcaaggt ttgattgagt tgccaaaaac
     2221 gctcattgtc tataaatgtt ccttcaaaaa aaccatacgt aaatggcgac catgcttgta
     2281 tggtaatatc attcaatcta caataatcaa gtactcctcc atttttatct atagcttgct
     2341 ctgtgttcat gtttacatta aaaccagcat ctattatccc actgtgcata atactaaatt
     2401 gcaattgatt gataagaagt ttgtgttgaa tgtgtttttg taataatgca agttgatgag
     2461 agttgtggtt actaacacca aaatggagca ctttgccttg ttgaaataag gtttcaaaag
     2521 cttcagctac ttcttcaggt tctaccagag cgtctggtcg gtgtagtagc aatatgtcta
     2581 tgtatgaggt ttttaagcga gaaagactgt ttt
//
LOCUS       DAOSMY010000227         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_39866_length_2587_cov_3.083728, whole genome shotgun sequence.
ACCESSION   DAOSMY010000227 DAOSMY010000000
VERSION     DAOSMY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39866_length_2587_cov_3.083728"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLD76_05485"
     CDS             <1..729
                     /locus_tag="PLD76_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU27488.1"
                     /translation="IDTAHGHSIYVIEKLKEAKKRFPQIDIVVGNIATGEAAKTLVAA
                     GADAVKVGIGPGSICTTRVVAGVGVPQLTAVYDVAKALKGTGIPLIADGGLRYSGDVV
                     KALAAGGFSVMIGSLVAGTDESPGETIIFNGRKFKTYRGMGSLEAMEKGSKDRYFQSG
                     EFDVKKLVPEGIAARVPYKGSLYEVIFQMVGGLRAGMGYCGARDIEELHNAKFTRITA
                     AGVKENHPHDVTITHEAPNYTRGE"
     gene            746..>2587
                     /locus_tag="PLD76_05490"
     CDS             746..>2587
                     /locus_tag="PLD76_05490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU27489.1"
                     /translation="MKKWIVFIFVSLSVSYTLAQKNTIVTIDQKPISIEEFEYYYNKN
                     NTNDLQQKMPVEEYMQLFVNFKLKVHQAYMLQMDTAQTFKQELEGYRNQLIKPYLTDN
                     EKIDDLVKEAYQHLLEDVDVSHILIRLNANPTPADTIAAYTKAMTAYKRVTNGESFEK
                     IAVEFSEDPTVQENKGRLGFITGMMVVYPFEKTAYTTPVGQVSAPIRTRFGYHIIKVH
                     DKRKTQGERLVAHILKRIPEDASEKQKAAIERSIKSIYEDLAKGADFQRLAQANSDDQ
                     SSANKGGEISWVGTGKTTPEFENAVFNLQNIGDISQPVKTEFGWHIIKLLDKRDIMTL
                     QEAYPLIEPRVKTDERASIIANSFIEKLKKTYNFKSYPANLHALHTLLAQPNDNILEQ
                     TKTYNLPIYTFADQVITQSDLIVFLHQQNYTKNPIDLSLLDILANQVANNTIIKYENS
                     MLEKKYPEFGLLMNEYKEGILLFNISNEMVWSKASKDIEGLTDFFEKNKKSYAWDKPR
                     FKGAIIYCDSKQSFKTAKKIAKATPEKELSTKLKQTLNSNEKNAVKIETGLFAEQTNP
                     VVDKLVFKKGKLEKNNDYPYIFTSGNIQKHYPDSYQDIRGIVINDYQN"
BASE COUNT         1006 a          465 c          454 g          662 t
ORIGIN      
        1 attgatactg ctcacggaca ctcaatttat gtaattgaaa aactaaaaga agcaaaaaaa
       61 cgttttcccc aaattgatat tgtagtagga aatattgcaa caggagaagc tgctaaaaca
      121 ttagtggctg ctggagccga cgctgtaaaa gtaggcatcg gtccaggttc catctgcaca
      181 acaagagtgg tagctggcgt aggtgtacct caattaacag ctgtatatga tgtagccaaa
      241 gccctaaaag gaacaggtat acctttaatt gcagacggag gacttcgcta ttcaggagac
      301 gttgtaaaag cattagccgc aggaggtttt tctgtaatga taggctcctt ggtggcagga
      361 acagacgaaa gtccaggtga aaccatcata tttaacggca gaaaatttaa aacatacaga
      421 ggaatgggtt ctttagaagc aatggaaaaa ggatctaagg accgctactt ccaaagtggt
      481 gagtttgatg taaaaaaatt ggtaccagaa ggcattgccg cacgagttcc ttataaaggc
      541 tctttatacg aggtaatttt tcagatggtt ggtggattac gtgctggaat gggctattgt
      601 ggagctcgcg atatagaaga attgcacaat gctaaattta cacgtattac tgctgctgga
      661 gtaaaagaaa atcaccctca cgatgtaact atcactcacg aagcacctaa ttacactaga
      721 ggagaataaa ctaaattttg attgtatgaa aaaatggatt gtgtttatat ttgtcagttt
      781 aagtgttagc tacaccctag ctcaaaaaaa cactatagta actattgatc aaaaacctat
      841 ttcaatagaa gaatttgaat attactataa taaaaataat acaaatgatt tgcagcaaaa
      901 aatgcctgtt gaagaatata tgcaattgtt tgtaaacttt aaactgaaag tacatcaagc
      961 ttatatgtta caaatggata cagcccaaac atttaaacaa gaactagaag gataccgtaa
     1021 tcaactaata aaaccatact taacagataa cgaaaaaatt gatgatctcg taaaagaagc
     1081 ttaccaacat ttactcgaag atgttgatgt tagccacatt ttaatacgac taaatgcaaa
     1141 cccaacccct gcagacacca ttgctgcata cacaaaagca atgaccgcat acaaaagagt
     1201 aacaaatggt gaatcattcg aaaaaatcgc tgtcgaattt tcagaagacc ctaccgtaca
     1261 agaaaacaaa ggaaggttag gcttcattac aggcatgatg gttgtctatc cttttgaaaa
     1321 aacagcatat acaactcctg tcggtcaagt ctcagcacca atcagaacaa ggtttggata
     1381 ccatattatt aaagtacacg acaaaagaaa aacacaagga gaacgattgg ttgcacatat
     1441 tttgaaacgt ataccagaag atgcatcaga aaaacaaaaa gcagctattg aacgctcaat
     1501 aaagagtata tacgaagatt tagccaaagg agctgatttt caacgtctag ctcaagcaaa
     1561 ctccgacgac cagtcatctg ccaacaaagg tggagaaatt tcttgggtag gaacaggaaa
     1621 aacaactccc gaatttgaaa atgctgtgtt taatctccaa aatattggag acatctctca
     1681 gcccgtaaaa acagaatttg gctggcatat tattaaattg ctcgacaaaa gggatattat
     1741 gacattgcaa gaagcatatc ccctaattga acctcgcgtg aaaactgatg aacgagcaag
     1801 cattattgcc aatagcttta tagaaaaact taaaaaaaca tacaacttta aaagttatcc
     1861 tgcaaaccta catgcattac atactctact ggcacaacca aacgataaca tactcgaaca
     1921 aacaaaaaca tacaacttgc caatttatac atttgccgat caggttataa ctcaatcaga
     1981 cctaattgtg ttcttacacc aacaaaacta cacaaaaaat cctattgacc tttctctcct
     2041 tgatatattg gcaaatcaag tagcaaataa cactattatc aagtacgaaa atagtatgtt
     2101 agagaaaaaa taccctgaat tcggactcct catgaatgaa tataaagaag gtattttatt
     2161 atttaatatt agcaatgaaa tggtttggtc taaagcctca aaagatatag agggactaac
     2221 tgattttttt gaaaagaaca aaaaaagtta tgcttgggat aaaccccgtt ttaaaggagc
     2281 tattatttac tgcgactcaa aacaatcttt taaaactgca aaaaaaatag ctaaagcaac
     2341 ccccgaaaaa gaactatcaa caaaacttaa acaaacattg aattcaaacg aaaaaaatgc
     2401 tgtaaaaatc gaaacgggac tttttgctga acaaacaaat ccagtcgtag acaaacttgt
     2461 atttaaaaag gggaaattag aaaaaaataa cgattatccc tatatattta ccagcgggaa
     2521 tatccaaaaa cattatcctg attcgtatca agatatacga ggcattgtga taaatgatta
     2581 ccaaaat
//
LOCUS       DAOSMY010000228         2551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_40609_length_2551_cov_3.747196, whole genome shotgun sequence.
ACCESSION   DAOSMY010000228 DAOSMY010000000
VERSION     DAOSMY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40609_length_2551_cov_3.747196"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /gene="dnaB"
                     /locus_tag="PLD76_05495"
     CDS             complement(<1..1227)
                     /gene="dnaB"
                     /locus_tag="PLD76_05495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU27490.1"
                     /translation="MEKTKKPRKKEPEYGKVPQYVVSEYGGKLPPQAIELEEVVLGAL
                     MLEKDAYVAVCEFLKKECFYKDVHQTIYEAITNLSLNQEPVDLLTVSQQLKRMGKLEE
                     VGGQSFISELTGKVASAANIEYHAQIIVQKYLSRELIRISSDIQKKAYDEKQDVDELM
                     QDAEGQLFEITQRNLKKDVTHIQPVISKAIDLIEIAANKQDGYTGIPSGFNGIDTITS
                     GWQKSDLIIIAARPAMGKTAFVLSMAKNMALDYEHPVAVFSLEMSNIQLVNRLIVNVA
                     EIEGDKIKRGKLAPFEWEQLRLRTATLQKAPIFVDDTPSLSVFELRTKARRLVKEHKI
                     QCIIIDYLQLMNASGMTYGSREQEVSIISRSLKGLAKELDIPIIALSQLNRGVEGRTG
                     ADKRPQLSDLRESGAIE"
     gene            1347..2162
                     /gene="ispE"
                     /locus_tag="PLD76_05500"
     CDS             1347..2162
                     /gene="ispE"
                     /locus_tag="PLD76_05500"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOU27491.1"
                     /translation="MITYPNAKINIGLFVTEKRTDGYHNLETIFYPIPLKDKIECHIS
                     DTNDSSLIISGIRIDGNTSDNLVMKAYNLLKLSHNLPNLNFTLEKNIPFGAGLGGGSA
                     DAAFVLTLLNTTYNLGLSIAELEYIASKIGADCPFFIQNKPVFATGIGNEFHPISFSL
                     SGYFLVLVKPPIHVSTPQAYKNINPQKPDFDLKTIHLLPIKEWKHVIFNDFETNVFIQ
                     HPEIEQIKDSLYHQGALYASMSGSGSSVFGIFPKQVNITNLFQNSFVWSGFLA"
BASE COUNT          785 a          445 c          419 g          902 t
ORIGIN      
        1 ttctattgct cccgattcgc gcaggtctga taattgaggg cgtttgtcag ctccagtacg
       61 tccttctaca ccacgattta actgtgataa agcaatgatt ggaatatcaa gttcttttgc
      121 caagcctttt aatgacctcg aaataatact tacctcttgt tcacggcttc catacgtcat
      181 gccgctggca ttcattaatt gcaagtagtc gattataata cattgtattt tatgctcttt
      241 aactaatcgg cgggctttgg ttcgtaactc aaatactgaa aggctagggg tatcatcaac
      301 aaaaattggg gctttttgca atgttgcagt tcgcaaacgc aattgttccc actcaaatgg
      361 agctagcttt ccacgtttaa ttttgtctcc ctctatttca gctacattta caattaaacg
      421 gttaactaat tgtatattag acatttcaag agagaaaaca gctactggat gttcataatc
      481 taaagccata ttttttgcca tagacagtac aaaagccgtt tttcccattg caggacgtgc
      541 agctataata ataaggtcgg atttttgcca tccagatgta atcgtatcaa tgccattaaa
      601 gcctgatgga atgcctgtat atccatcttg tttgttggca gcaatttcaa tcaaatcaat
      661 agctttgctt atgactggtt gaatgtgtgt aacgtctttt ttaaggtttc gttgtgtaat
      721 ttcaaaaagc tgtccttcag catcttgcat taattcatca acgtcttgct tttcatcata
      781 cgccttcttt tggatatcag acgagatacg tattaattct ctcgacaaat acttttgaac
      841 aataatttga gcatgatatt ctatattagc ggcagaagcc actttccctg ttagttctga
      901 aataaaagat tgtcctccta cttcttctaa ttttcccata cgttttaatt gttgggatac
      961 agttagtagg tcaacaggtt cttgatttaa cgatagattg gtaattgctt cgtatattgt
     1021 ttggtgtaca tccttgtaaa aacattcttt tttgagaaat tcgcacaccg ctacataagc
     1081 atctttttct aacatcaaag cccctaaaac cacttcttcc aattcaattg cttgaggtgg
     1141 cagttttcct ccgtattcag atacaacgta ctgaggtact tttccgtatt ctggttcttt
     1201 tttgcgtggt ttttttgtct tttccatggt ctttgctaat gaggttcaaa aatagcactt
     1261 tttttcatat ttagaaaagg tatttttgaa caatcctcat tctgaattgt tgaaaagtta
     1321 tacctttgca ctattattct atatatatga ttacgtatcc gaatgcaaaa ataaatattg
     1381 ggttgtttgt taccgaaaaa cgaaccgatg gttaccacaa tttagaaacg atattttatc
     1441 ctattccatt aaaagataaa atagagtgtc atatttcgga tacaaacgat tcttcactta
     1501 tcatatcagg tatccgtata gatggcaata cctcagataa tttagtaatg aaagcataca
     1561 atttgcttaa attatcgcac aacttaccaa atcttaattt tactttagaa aagaatatcc
     1621 cttttggtgc aggattggga ggtggttcgg cagatgctgc ttttgttctt accttattaa
     1681 atacgaccta taatttaggt ttatccattg ccgaattaga atacattgca tccaagattg
     1741 gtgcagattg cccttttttt atacagaata aaccagtatt cgctacaggg attggtaatg
     1801 agtttcatcc catttcattt tctttgtctg gttatttcct tgttttagta aaaccgccta
     1861 ttcacgtatc aactccgcag gcatataaaa atatcaatcc tcaaaaacct gattttgatt
     1921 taaaaacaat tcatttacta ccaataaaag aatggaaaca tgttattttt aatgattttg
     1981 aaacgaatgt ttttattcaa cacccagaaa ttgaacagat taaagatagt ttgtaccatc
     2041 agggagcttt atatgcatcc atgtcagggt caggttcttc tgtctttgga atcttcccaa
     2101 aacaggtaaa tattacaaat ttgtttcaaa attccttcgt ttggtctggt tttttagcct
     2161 aaaagcctat ttttcttctc atttttatca tattatttga cgaaagttgt aaaatatatg
     2221 ttttacagca tatatttgta atgcattcga gagcgaatgg ctaacaaatg aataattaaa
     2281 ataatgaaag gagacaaaat tatgacacag aatcagattt ttgactcttt agttttagaa
     2341 aattaagggt caataaaatg gaaatgattg ctaaccgcaa gattcgttac aaaaaagtgt
     2401 tgaaccaatt aaatttacaa ctatgtacaa agcgatggca tataaaggac ttggagattt
     2461 aaaattattt gatattatct tgggacattc aagttctgaa agaaaaataa gcgaaaagaa
     2521 aaaaagtaaa tagtgtttct aaatgtcgga a
//
LOCUS       DAOSMY010000229         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_40681_length_2548_cov_4.283995, whole genome shotgun sequence.
ACCESSION   DAOSMY010000229 DAOSMY010000000
VERSION     DAOSMY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40681_length_2548_cov_4.283995"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLD76_05505"
     CDS             complement(<1..922)
                     /locus_tag="PLD76_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU27492.1"
                     /translation="MAKGDLSYNRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTSWDYQNEKYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVAAFCAVGGVEPQSETVWRQADKYKVPRIGYVNKMDRSGADFFDVVRQVKEVLGA
                     NPCPIQIPIGAEEKFQGVVDLVKMKAIFWHDETMGAQYSIEEIPANLLSEAKEWREKM
                     LEVVAEFDDVLMEKFFDDPSTITEDEIKAAIRKGTISMQINPMICGSSFKNKGVQTML
                     DAVCAYLPSPLDTPEIIGQDVNDAKKQVVRH"
     gene            complement(949..1425)
                     /gene="rpsG"
                     /locus_tag="PLD76_05510"
     CDS             complement(949..1425)
                     /gene="rpsG"
                     /locus_tag="PLD76_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU27493.1"
                     /translation="MRKAKPKKRIVLPDPIFGDVMVTRFVNHLMYDGKKTTAFTIFYT
                     ALENVKGKLPNEEKSSIDIWKKALENITPQVEVKSRRIGGATFQVPTEIRPERKESIS
                     MKNLILYSRKRGGKTMADKLAAEIVDAFNLQGASFKRKEDMHRMAEANRAFAHFRF"
     gene            complement(1586..2002)
                     /gene="rpsL"
                     /locus_tag="PLD76_05515"
     CDS             complement(1586..2002)
                     /gene="rpsL"
                     /locus_tag="PLD76_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU27494.1"
                     /translation="MPTIQQLVRKGRAKQIDTSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNSYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRKQRRSKYGAKRPKPGQPAAAPAKGGKKK"
     gene            complement(2160..2471)
                     /locus_tag="PLD76_05520"
     CDS             complement(2160..2471)
                     /locus_tag="PLD76_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOU27495.1"
                     /translation="MENSNNGFSIEIADDIAEGVYSNLAIIAHSTSEFVIDFARLMPN
                     TPKAKVKSRIILNAEHAKRLLLALQENITKFEKQNGKIILSEQKNPPIPMNFGGMKGE
                     A"
BASE COUNT          699 a          487 c          431 g          931 t
ORIGIN      
        1 gatgtcttac tacttgtttt ttagcatcat ttacatcttg tccgataatt tcgggagtat
       61 ctaatgggct tggcaagtat gcacaaacag catcaagcat agtttgaaca cctttatttt
      121 tgaaagaaga cccgcaaatc ataggattga tttgcatgct tattgtacct ttgcgaatag
      181 ctgctttgat ttcatcttca gtgatagtgc taggatcatc aaagaacttt tccataagaa
      241 catcatcaaa ttcagcaacc acttcgagca ttttttctct ccattctttg gcttcgctca
      301 acaaattagc ggggatttct tcaatactat attgagctcc catcgtttca tcatgccaaa
      361 aaattgcctt cattttcaca aggtctacta caccttgaaa tttctcttca gcaccaattg
      421 gtatttgaat aggacaagga tttgctccta atacctcttt aacttgacga acaacgtcga
      481 agaaatcggc accagagcgg tccattttat ttacataacc gatgcgaggc actttgtatt
      541 tatctgcttg acgccatact gtttctgatt gaggttcaac acctccaaca gcacaaaatg
      601 ctgctacagc tccgtctagg atacgcaacg aacgttcaac ctctacggta aagtcaacgt
      661 gtcctggggt gtcaatcaaa tttattttgt atttttcatt ttgataatcc caactagttg
      721 tagtagctgc agaagtaatt gtaatacctc tttcctgctc ttgctccatc cagtccatgg
      781 tagcagcacc atcgtgtact tcaccaattt tgtgggttaa ccctgtataa aatagaatac
      841 gctcggaagt tgttgtttta ccagcatcaa tgtgagccat gataccgata ttccgattgt
      901 aacttaaatc tccttttgcc attttagtat atcttcagat gttaaatatt aaaaacgaaa
      961 atgggcgaaa gctctgtttg cttcagccat acgatgcata tcttctttgc gtttgaaaga
     1021 agcaccttgc agattgaatg catctacaat ttcggcagct aatttatcag ccatcgtttt
     1081 tcctcctctc ttgcgagaat agagtattaa gtttttcatt gaaatggatt cttttctttc
     1141 tggacggatt tcagtaggaa cttgaaatgt agctccaccg atacggcgag atttcacttc
     1201 tacttgaggg gtgatgtttt ctaaagcttt tttccagata tcaatagacg acttttcttc
     1261 gttaggtaat ttacctttaa cgttttctaa agctgtataa aagatagtaa atgcggtggt
     1321 ttttttacca tcatacatta agtggttaac aaaccttgta accattacat ctccgaaaat
     1381 tggatcaggt aatacgatcc gtttttttgg ttttgctttt ctcatttttt ttaaaatttt
     1441 tccgttttgt atttggttgc cattgtgtct tcaaaaaatt acgcttaatt tttttactca
     1501 accgataaaa tgtctaaatc caaaatatgc aaaacagtag taattaactg ttgtttgttt
     1561 ttcttttaat tttcacaccg aaggattatt tctttttacc tcctttagct ggtgcagcag
     1621 ctggttgacc tggttttggg cgtttagctc cgtatttaga acgtctttgt ttgcgaccat
     1681 taacgccagc agtatctaat gttccacgta caatgtgata acgaacccct ggtaaatctt
     1741 taacacgacc accacgaatt agcacaattg agtgttcttg caagttgtgt ccttcacctg
     1801 gaatatatga gtttacttcc ttttggttag ttagacgaac tcttgctact ttacgtaaag
     1861 ccgagttagg ttttttggga gttgttgtat acacccttac gcacacacca cgacgttgag
     1921 gacaagagtc caacgctgga gacttgcttg tgtcgatctg tttagccctt ccttttctta
     1981 ctaattgctg aattgtaggc atttttcttg tttttgttat aaattattag acttcttttt
     2041 gaacccattt tttgtaaatt cgggttgcaa agatacacaa acttttgtac taaacaatat
     2101 tttagtataa aaaaatacaa atttatttga gaaacaatta tttaatagaa gcatacgaac
     2161 tatgcttctc ctttcatccc accaaaattc atgggaatag ggggattttt ttgttctgaa
     2221 aggattattt tcccgttttg tttttcaaat ttagtaatat tttcttgtaa tgctagtaaa
     2281 agtcttttag catgctcggc atttaagata atgcgagatt tcactttagc tttgggggta
     2341 ttgggcatta atcgagcaaa gtctattaca aattcgctgg ttgaatgtgc tataattgct
     2401 aaatttgaat acacaccttc tgcaatgtcg tctgcaattt ctatactaaa accattgttt
     2461 gaattttcca ttctattttg ttttaaaatt atgtaattaa gaaagatgtt ttttctaaaa
     2521 aaaccttatg tttctactat tttgtatc
//
LOCUS       DAOSMY010000230         2540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_40843_length_2540_cov_3.331590, whole genome shotgun sequence.
ACCESSION   DAOSMY010000230 DAOSMY010000000
VERSION     DAOSMY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40843_length_2540_cov_3.331590"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /gene="sufD"
                     /locus_tag="PLD76_05525"
     CDS             <1..800
                     /gene="sufD"
                     /locus_tag="PLD76_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOU27496.1"
                     /translation="LAVNHNLIIVEEGASVKLLVCDHASNEKKYLANRLTEIFVGKNA
                     SYEHYKIENTHNQTMNLGTVLVHQDDDSRFLSNTLTLHNGITRNNITVHVDGQNAEAI
                     VGGIDIGDKTQKIDNYVSMNHLKPNSTSLQLFKYILDDQAKGVFTGKIIIKEDAQKIA
                     AYQTCKTICLTNTATINTKPQLEIFADDVKCSHGATVGQLDETALFYLRSRGIPINEA
                     KLLLMYAFVHDVLEHISVDAVKDRIRLLVEKRLRGELSKCNGCVICK"
     gene            819..2042
                     /locus_tag="PLD76_05530"
     CDS             819..2042
                     /locus_tag="PLD76_05530"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOU27497.1"
                     /translation="MSINNQLIRQDFPILLDKIYNKPLVYFDNAATTQKPMCVIDRIV
                     HGYTHGNANIHRGVHFLSQKATEEHENARTTVQHFIHAARSSEIIFTRGTTESINLVA
                     FSFGETFCQPGDEIIISAMEHHANIVPWQMLKDRKGIVLKVIPMLPDGTLDMPAFSKL
                     LNNKTKLVAVTHVSNVLGTINPIQEIIQLAHNSNVPVLIDGAQAVPHIAIDVQQLDVD
                     FYVFSAHKVYGPTGIGVLYGKEEWLNKMQPYQGGGEMIAKVTFEKTTYNELPFKFEAG
                     TPDYIGSTALATALEYIQQIGLNAIATYENDLLQYATAKLLQVPGLKIYGEASHKSSV
                     ISFLVQGIHHYDMGTLLDKLGIAVRTGHHCAQPIMDYYGIEGTVRASFAFYNTKEEVD
                     KLIEGVNRVVSMFKI"
     gene            2054..2479
                     /locus_tag="PLD76_05535"
     CDS             2054..2479
                     /locus_tag="PLD76_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOU27498.1"
                     /translation="MTIQEKQDIIIEEFSVFDDWFDKYALIIEYANKLKPLDETKKTP
                     QNIIEGCQSRVWIDASINEKGQVVFVGDSDAIIVRGILALLLYVLSEHTPQEIIESEL
                     YFIEKIGLKEHLSPTRSNGLVAMMKQLKLYSIAFSSKKG"
BASE COUNT          908 a          403 c          488 g          741 t
ORIGIN      
        1 tattggcagt gaatcacaat cttattattg tggaagaagg tgcaagtgtt aaattgttag
       61 tatgcgatca tgcttcaaat gagaaaaaat acctagccaa tagattaaca gagatttttg
      121 ttggcaaaaa tgcttcttac gagcactata aaattgaaaa tacccacaat caaacaatga
      181 atcttggtac cgtgttagtg catcaagacg atgatagtag atttttatca aataccctta
      241 ctttgcataa cggaataacc cgtaataata tcacagtaca tgtagacgga caaaatgccg
      301 aagcaatagt aggagggata gacattggag ataaaactca gaagatagat aactatgtgt
      361 cgatgaatca cttaaaacca aactctacta gtttgcaatt gtttaagtat atactggatg
      421 atcaggcaaa aggtgtgttt acaggcaaaa ttattataaa agaagatgct caaaaaattg
      481 ctgcatatca aacgtgcaaa actatatgtt taacaaatac ggcaacaatt aacacaaaac
      541 cacaattgga aatttttgct gacgatgtga aatgtagtca tggtgcaacc gtaggtcagc
      601 tcgatgaaac agctcttttc tatctccgtt caagaggtat tcctattaat gaggctaaac
      661 tgttacttat gtacgcattt gtacacgatg tgttagaaca catttcagtc gatgcagtca
      721 aagaccgtat tagattgtta gtagaaaaaa gattacgggg agaattgtct aaatgtaatg
      781 gatgtgtaat ttgtaaataa ggatgataaa taagaaaaat gagcataaat aatcaactta
      841 tccgacagga tttcccaatt ctgttagata aaatttacaa taaaccatta gtttacttcg
      901 ataatgctgc taccacacaa aaaccaatgt gtgtgattga tagaattgta catggatata
      961 cgcatgggaa tgcaaatatc cacagaggtg ttcatttttt aagccaaaaa gcaaccgaag
     1021 aacatgaaaa tgcacgtaca actgtgcaac attttattca tgccgcaagg tcgagtgaaa
     1081 taatttttac tcgaggtact actgagtcta ttaatttagt ggcattttct tttggcgaaa
     1141 cgttttgcca accgggggat gaaattataa tttctgctat ggaacaccat gcaaatattg
     1201 ttccttggca aatgcttaaa gatagaaaag ggattgtgct taaagttatt ccaatgctac
     1261 cagatggaac tttggatatg cctgcttttt caaaactatt gaataacaaa acaaaattag
     1321 ttgcagttac tcatgtttca aacgtgctag gaacaataaa cccaattcaa gaaattattc
     1381 aactcgctca taattccaat gttccagtgt tgattgatgg agcacaagct gtaccacata
     1441 ttgctattga tgtccaacaa ttggatgttg atttttacgt gttttctgca cacaaggtgt
     1501 acggtcctac tggcattggc gtattgtatg gaaaagagga atggttaaat aaaatgcaac
     1561 cataccaagg aggaggagaa atgattgcaa aggttacctt cgagaaaact acttataacg
     1621 aacttccgtt caagttcgaa gcaggtacgc ccgattatat tggttctaca gcccttgcta
     1681 cagccttaga atatatccaa caaatcggat taaacgctat tgccacatac gaaaacgact
     1741 tactacaata tgcaacagcg aaactgttgc aagtaccagg gttaaaaatt tatggcgaag
     1801 catcgcataa aagcagtgtg atttcttttt tagtacaagg cattcatcat tacgatatgg
     1861 gaactctgct cgacaagctc ggtattgcgg tccgaacagg tcatcattgt gcacagccaa
     1921 ttatggatta ttatggcata gagggaacgg taagggcttc atttgccttc tataatacca
     1981 aagaagaggt agataaactc atagaaggag taaatagggt ggtatctatg tttaaaattt
     2041 aatttgtaag ggtatgacaa ttcaagaaaa acaagatata ataatagagg aattttcagt
     2101 ttttgacgat tggtttgata aatatgcact cattattgag tatgccaata aattgaaacc
     2161 tcttgatgag acaaaaaaaa caccacaaaa catcatcgaa ggatgccaaa gtagggtatg
     2221 gatagatgct tctattaacg aaaaaggaca agttgtgttc gttggagata gtgatgcaat
     2281 tattgtaaga ggtattttag cattactttt atacgtatta tctgaacata ctccgcaaga
     2341 aattatcgaa tcagaattat attttataga aaaaattgga ctaaaagaac acctttcgcc
     2401 caccagatcc aatggtttag tggcaatgat gaagcaactc aagctctaca gtatagcttt
     2461 tagcagtaaa aaaggataaa ataatgttgt attatatcct tgattttaat gcagttaagt
     2521 aatttggata aaaatagatt
//
LOCUS       DAOSMY010000231         2531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_41042_length_2531_cov_4.288772, whole genome shotgun sequence.
ACCESSION   DAOSMY010000231 DAOSMY010000000
VERSION     DAOSMY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41042_length_2531_cov_4.288772"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..949)
                     /locus_tag="PLD76_05540"
     CDS             complement(149..949)
                     /locus_tag="PLD76_05540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27499.1"
                     /translation="MKISLIISIYKNVPFLKLVLESLQVQSYQDFEIIISEDGEDENV
                     RQFVASYPFKQSYQHLTQKDDGWQKNKALNNAIRKSQGDWLIFIDGDCVLHKRFVEFH
                     AKMADEHLILAGKRVKLSAETSNKLLSNPTLLPTLSRYLYIHFWKSGRYLEEALFIHP
                     QGLLGWITRIRTMKHLKGCNMSFSKKALYAINGFDEDYTLPAIGEDVDILWRLQQAGY
                     QLKSVRNMAVMYHLHHKESWVSQEENKEKMRQKMEQNTYICKNGIQKI"
     gene            1068..1832
                     /locus_tag="PLD76_05545"
     CDS             1068..1832
                     /locus_tag="PLD76_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU27500.1"
                     /translation="MGKIIALANQKGGVGKTTTTVNLGASLAVLEKKVLIVDADPQAN
                     ASSGLGIDIEKLEYSIYECLINNVPVSKAIQKTPIEGLHILPSHIDLVGAEIEMLKLD
                     NREKFLRTMLETIKNEYDFILIDCSPSLGLITVNSLTAADSLIIPVQAEYYALEGISK
                     LLNTVKIIKSRLNPSLDIEGFLVTMYDSRLRLANQVYAEVKKHFQDMVFETVIQRNIR
                     LSESPSHGLPVVLYDADSKGAQNHMQLAAEIIKNNS"
     gene            1857..>2531
                     /locus_tag="PLD76_05550"
     CDS             1857..>2531
                     /locus_tag="PLD76_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU27501.1"
                     /translation="MNKKSGLGRGLGALIEMETVTTSGSSSISEVDITKIQANPDQPR
                     TYFDEEALQELATSIKEVGIIQPITLRQVSSDTYQIIAGERRYRASKIAGLTKIPAYV
                     KTVDDEAVMEMALVENIQREDLNAIEIALSYQKLIEISGYTQETMSERVGKKRSTIAN
                     YLRLLKLPAEIQLGITNKKIDMGHARALVTISDASVMLQVYEKIVKEGLSVRKVEELV
                     KNLQSLE"
BASE COUNT          833 a          402 c          483 g          813 t
ORIGIN      
        1 agaaaaaata gaatgtgttg attgtgaatt gtctatatga ataatctcat agtcaacacc
       61 tacggtttcg tgaatgttgg ctgttaaaat agaagataat tcggcaaatt ttgaacaaat
      121 aataatggat agcatgcgtt ctgtatagtt agattttttg tattccgttt ttgcaaatat
      181 aggtattttg ttccattttt tgcctcattt tttctttgtt ttcttcttga cttacccaac
      241 tctctttgtg gtgtaggtgg tacataacag ccatattgcg tactgatttc agttggtagc
      301 ctgcttgttg taaacgccat aaaatatcca catcttcgcc aattgcaggt agggtatagt
      361 cttcatcaaa tccattaatt gcatatagtg ctttttttga aaaagacatg ttgcatcctt
      421 ttaaatgttt catcgtgcgt atacgtgtta tccaacccaa cagaccttgt ggatgaataa
      481 ataaagcttc ttctaaatat cgaccagatt tccaaaagtg gatatataaa taacgcgata
      541 aggttggcaa taaggtgggg tttgaaagca gtttgttact ggtttctgcc gataatttaa
      601 cacgtttgcc agctaaaatt aggtgctcgt cagccatttt tgcatgaaat tcaacaaatc
      661 gtttgtgaag cacacaatca ccatcgataa aaatgagcca atcaccctga gattttctta
      721 ttgcattgtt cagtgcctta tttttttgcc atccatcatc tttttgtgta agatgttgat
      781 aactttgttt aaatgggtat gaggctacaa attgacgcac attttcgtct tctccatctt
      841 ctgatataat aatttcaaaa tcctgatatg attgaacttg caaggattca agcaccagtt
      901 tcaaaaaagg tacattcttg tatatagata tgatgaggct tattttcatg gtgcaaaagt
      961 actataaaaa tagatgtaag actaaaaatg ttgaaaaaat aaacattcaa cctcaaaatt
     1021 tttgctacat ttgcgtttgg aaaaaacgta aaaattttaa atacaatatg ggtaaaatta
     1081 ttgccttagc aaatcaaaaa ggtggagtag gaaagacaac aaccactgta aacttaggag
     1141 cttcgttggc ggtactcgaa aagaaagtgt tgattgtaga tgcagatcca caagcaaatg
     1201 cctcttcggg cttaggtatt gatattgaaa aattagaata cagtatttat gaatgtttga
     1261 taaataatgt gcctgtaagc aaagccatcc aaaaaacacc catcgaaggc ttgcatattt
     1321 tgccctcgca tattgatttg gttggtgctg agattgagat gttgaagctc gataatcgtg
     1381 aaaagttttt acgtaccatg ttagaaacga taaaaaatga gtatgatttt atcctaattg
     1441 attgttctcc ttcgctaggc ttgattacag tcaattcgct tacagcagca gattccctaa
     1501 ttatacctgt acaagccgaa tattatgcgc ttgaaggaat aagcaagctg ttaaataccg
     1561 taaaaattat taaaagtagg ttgaacccat cgcttgatat tgaaggcttt ttagtaacca
     1621 tgtacgattc tcgtttgcga ttagccaatc aagtatatgc tgaggttaaa aaacattttc
     1681 aagatatggt gtttgaaaca gttatccaac gtaacatacg cttgagtgaa tcgcctagtc
     1741 atggcttgcc tgtagttttg tatgatgcag attctaaagg agctcaaaac catatgcaat
     1801 tggcggctga gattattaaa aataattcat aatatattaa gttataatcc cgtacaatga
     1861 ataaaaaatc aggtttaggc agaggcttgg gtgctctcat cgaaatggaa acggtaacta
     1921 ctagtggttc atcctcgata agcgaggtgg atataactaa gatacaagcc aatccagacc
     1981 aaccacgtac ttattttgat gaagaagctt tgcaagaatt agcaacatca attaaagaag
     2041 ttggtatcat tcagccaatt actttgcgtc aagtgtctag cgatacgtat caaattattg
     2101 ctggagaaag acgttatcgt gcttcaaaaa tagctggttt aacaaaaatt cctgcttacg
     2161 ttaaaaccgt agatgacgag gctgtaatgg aaatggctct ggttgaaaat attcagcgtg
     2221 aagatttaaa tgccatagaa attgcattga gttatcaaaa attgatagag atttctggtt
     2281 atacccaaga aaccatgagc gagcgtgtag gcaaaaaacg ttctacaata gctaattatc
     2341 ttcgtttgct taaattacct gctgaaattc aactaggtat tacaaataaa aaaattgata
     2401 tggggcatgc acgtgcattg gttacaattt ctgatgcaag tgtgatgttg caagtatatg
     2461 aaaaaattgt gaaagagggt ctttctgttc gaaaagtaga agaattagta aagaatcttc
     2521 aaagtcttga a
//
LOCUS       DAOSMY010000232         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_41238_length_2522_cov_2.653831, whole genome shotgun sequence.
ACCESSION   DAOSMY010000232 DAOSMY010000000
VERSION     DAOSMY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41238_length_2522_cov_2.653831"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2304..>2522
                     /locus_tag="PLD76_05555"
     CDS             2304..>2522
                     /locus_tag="PLD76_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27502.1"
                     /translation="MPIRVEISKEGSAWSGFWLKRTELKGVLRKPDYNAFLNYVWDLV
                     GEIPMPTTDCPYVAETKEEGMANWLGLKF"
BASE COUNT          804 a          452 c          528 g          738 t
ORIGIN      
        1 aaaaatattc cctaaaaata ttccctaaaa atattcccta aaaatattcc ctaaaaatat
       61 tccctaaatt ttagggaaaa aatattccct aaaaatattc cctatttttt agggaaaaaa
      121 tttctattgg gggacctcca tcaaaaaatt ttatcaacac attgacatat atcaatagtt
      181 atgctataag atatattatg aaagtattac agcattacct aaactcattg catttgtatt
      241 gctttttccg taaaatcgga tttagcaagc agatagcaat gataatggct aagacctgta
      301 gcatagataa gctactctac aggtaatacg cacctgttca aacttgcaca tctgcaagtt
      361 attgaaatgc ttaggtgtga ggtatttctc caccgcacct atgcaaaatg acataggacg
      421 gtggagccga aaattgaatt tcccccatag gggaaatttg ccttgctgat ttacaagtta
      481 ttgaaatgtt taggggtgag gttggggcat agtcgcccta ccctgtccac cgcacctata
      541 caaaatgaca tagggcggta aaaaaatctt gacaatttta tattaatgta gtatgctgta
      601 cagaatgaga tataataaat acaaaaaaca acggaggtgt agtatgtttg aaggcaaaac
      661 ttgtgacacc tgcatgatgc aggaattgaa gtcctacaca actgcctgtt gtaatgggtg
      721 cgagttggta gagcaacagg caatcctaat agctcggtta ttaatcgatg ccgagcaggt
      781 gttcggaggt gagatatggt aatagcaaca ctaaaaaccc tagcagtatg ggctatgctg
      841 tacgctatat tttatagcgt actaatcacc accctactgt tctcatagta ttgtactaaa
      901 tttagtacaa tcgcaatcct gaaaaattgc gattttgaca tattttttcg atttttcagg
      961 gctgggacac gatttttgta ctaaatttag tacagagtag gaatcaaaaa aatgcaaatc
     1021 cagcaaaaag cacgtccttt gacgccctgt aggacaggat tttcagccaa atcctatcaa
     1081 aatgggccca ttcctgccct gtcctacagt atcctgccca atccacccct atatatatac
     1141 gaggtgccca gttggatagg tatgggaccc ctatatatat acgaggtgcc cagttggaca
     1201 gggtaggtac cctaccctgt cctgtccaca gtttaatata ctaaacaaaa agtatagtac
     1261 tatactggaa gtttaataaa ttaaacacgt tagggcttga gcccctgccc tacaggatag
     1321 ggtataacag ggctatcagc cctatagcaa gagtatccta cctgaaaatc tgggcaaaat
     1381 cgtatccctg gtagccagtt tagcatatta aacttggagt ttaatactgt tctaaacttg
     1441 gagtttagca ctaaattttt tgtttaatat aataaactca agcctccaca ggcggtagga
     1501 tgcaaggacg cagagaggac gggaatccat cctggaattg gctatggatt gtggtttggt
     1561 gcgggctttg tggatagcag taaaatcaat agaaggggag ctttgccgtt tgtggatgct
     1621 tgaggagagc caatcccctc caccaccaac cacagcataa caattactta tatactatat
     1681 atagccctgt aattacggta ggtttctacc cacatgatag catatttttt accctatttt
     1741 acatatgtag tcatatagtt attttttgtt aggtttatac tttttttgta tcaggagcgg
     1801 agttattttc ccataagaaa aaaatttttt atttttttgt tgacatcttc tatataataa
     1861 tgtaagtata atacatcagc ttctttaagc tgaaataact ttcggaggtg tagcatgaaa
     1921 tacgataaaa acgagttatt tgaattaatc aatgatgact catgggacga aattgtgttt
     1981 gaggacgaca ttgtggactg ccttacagta gtaaggcgtg gattcggcat gtttcgtaat
     2041 actaacgata tggaagatgg cttccatata gaaactttat aatttaagga gtcagatatg
     2101 ttttggggaa aaagtgatag ggcaatggca aataaagaga cgtacgtcat caggtacata
     2161 ttctctaagg atgacgtagt tgaacacatg gtatattgta cagcaaaaac cctggatagt
     2221 tgggttagag cttttggtaa ggtctttacc gaacaaaagc ctatgagggt agagaccgaa
     2281 atcttaccat cgaggaagta atcatgccta ttcgagtaga gatttccaaa gaagggagtg
     2341 cgtggtcggg tttctggctc aagaggacag agctgaaagg ggtgcttagg aaacccgact
     2401 acaatgcatt cctgaactac gtatgggacc ttgtaggtga aatacctatg cctacaacag
     2461 attgtcccta tgtagctgaa actaaagagg aagggatggc aaactggttg ggattgaagt
     2521 tt
//
LOCUS       DAOSMY010000233         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_41334_length_2517_cov_3.059301, whole genome shotgun sequence.
ACCESSION   DAOSMY010000233 DAOSMY010000000
VERSION     DAOSMY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41334_length_2517_cov_3.059301"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2372
                     /gene="pflB"
                     /locus_tag="PLD76_05560"
     CDS             156..2372
                     /gene="pflB"
                     /locus_tag="PLD76_05560"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOU27503.1"
                     /translation="MNFKNGKWNNAVDVRDFVSLNITPYDGDSGFLCSPTQRTLDLWQ
                     ICKDAISEERANNGVRSIDNELVSTITSHAPGYIDKEKELIVGLQTDQLLRRAMKPYG
                     GIAVVEKACSENGVEVSPFVKEVFTKYAKTHNDGVFDVYTPEIRTFRSLGVLTGLPDN
                     YARGRIIGDYRRVALYGVNFLIQQKKKDLESLLGPMSDERIRLREEVSEQIKALYAII
                     EMGKSYGFDLSRPAQNAIEAVQWVYMGYLAAVKEQDGAAMSLGNVSSFLDIYIEQDIA
                     EGKISEEFAQELIDQFVMKLRMVRHLRMDAYNEIFAGDPTWVTESIGGALADGRHKVT
                     KTSYRFLQTLYNLGPSPEPNMTLLWSEKLPENFKKFCAQVSIDTSSIQYENDDLMQST
                     RCSDDYGIACCVSYQEIGKQIQFFGARTNLAKTLLLAINEGRCELSGQQVLTGIPKLK
                     SEVLDYEEVMEVFKLTMKEVARVYSETMNIIHYMHDKYYYEKAQMALIDTNPKINLAY
                     GVAGLSIVADSLSAIKHAKVTPIRDEHGVAKSFKIEGEFPFYGNDDDRVDAIAVEVPA
                     YFNQLLKKLPTYKNAEPTLSILTITSNVVYGKKTGATPDGRAHGVPFAPGANPMHGRD
                     HSGAIASLSSVSKLDYSEALDGISNTFSIVPKSLGPTDEERADNLVSIIDGYFNKEAH
                     HLNVNVLNREMLEDAMEHPENYPQLTIRVSGYAVNFVRLTRAQQQEVISRSFFESL"
     gene            2390..>2517
                     /locus_tag="PLD76_05565"
     CDS             2390..>2517
                     /locus_tag="PLD76_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOU27504.1"
                     /translation="MINVHSVESLGTYDGPGIRLVLFLQGCNFKCLYCANPDTIEP"
BASE COUNT          805 a          423 c          519 g          770 t
ORIGIN      
        1 cggtattttg taatacaatg gtaacattta acttaaaaat cactaaatag aaatgtcttt
       61 ttgctaaaat aatatgttgt acaacataaa tatagtaatt ttgtggcatc aaattaccag
      121 cataattata tataatactc taaatacttt acactatgaa ttttaaaaac ggaaaatgga
      181 acaatgcggt tgatgtaaga gattttgttt ccttaaatat tactccatac gatggagata
      241 gtggcttttt gtgttcacct acacaacgta cactcgattt gtggcaaata tgtaaggatg
      301 ccatttcaga agagcgtgcc aataatggcg ttcgctccat tgacaatgaa ttagtatcta
      361 caattacttc gcatgctcca gggtatattg ataaggaaaa agagttaatt gtagggcttc
      421 aaacagacca actacttcgc agagctatga aaccctatgg aggtattgcc gtagtagaaa
      481 aagcatgtag cgaaaatggt gttgaggtga gtccatttgt aaaagaagta tttactaaat
      541 atgcaaaaac gcacaatgat ggtgtatttg atgtgtatac acctgaaatt agaacatttc
      601 gttcgttagg tgtgcttacg ggtttgcccg ataattatgc tcgtggtcgt ataataggcg
      661 attatcgtcg tgtggcatta tatggtgtta attttcttat tcaacaaaag aaaaaagatt
      721 tagaaagctt gcttggacct atgagcgatg aaagaattcg gttgcgtgaa gaggtttctg
      781 agcaaatcaa agccctgtat gccattatcg aaatgggtaa atcctatgga tttgatttgt
      841 ctcgtccagc acaaaatgcg atagaagctg tacaatgggt atatatgggt tatttagctg
      901 ccgtaaaaga acaagacggt gcagctatgt cgttgggtaa tgtgtcgtcg tttttagata
      961 tttacattga acaagatatt gctgaaggaa aaatttcaga agagtttgct caagagctta
     1021 tcgaccagtt tgtgatgaaa ttgcgtatgg tacgtcattt gcgtatggat gcgtataacg
     1081 aaatttttgc tggcgaccca acttgggtaa ctgagtctat tgggggggca ttggctgacg
     1141 gacgccacaa ggtaaccaaa acatcatacc gtttcttaca aacattatat aacctcggac
     1201 catcacccga gcctaatatg accttgttat ggtcagaaaa attacccgaa aactttaaaa
     1261 aattctgtgc tcaagtatct attgacacat cttctataca gtacgaaaat gatgatttga
     1321 tgcaaagtac acgttgcagt gatgattatg gtattgcttg ttgtgtatcg taccaagaaa
     1381 ttggcaaaca aattcaattc tttggtgctc gtaccaattt agcaaaaaca ttattgttgg
     1441 ctatcaacga aggtcgttgc gaactttctg gtcaacaagt gctaacaggt attccaaagt
     1501 tgaagtcaga agtgcttgat tatgaggagg taatggaagt gtttaaactt accatgaaag
     1561 aagtggcacg tgtgtatagc gaaacaatga acattatcca ctacatgcac gataaatatt
     1621 attacgaaaa agcacaaatg gctttgattg ataccaatcc aaaaattaat ttagcctatg
     1681 gtgtagctgg tttaagtatt gtagccgatt cattatctgc catcaaacat gctaaagtaa
     1741 cccctatacg tgatgaacat ggagtggcaa aaagttttaa aattgaagga gaatttcctt
     1801 tttatggcaa tgatgacgac cgtgtagatg ccattgctgt agaagtacct gcatatttca
     1861 atcaattatt gaaaaaattg ccaacgtata aaaatgctga acctaccttg tctattctta
     1921 ccattacatc caatgtagtg tatggtaaaa aaacaggagc tacgcctgat ggacgtgctc
     1981 atggggtgcc ttttgctcct ggagctaatc ctatgcacgg acgcgaccat agtggagcga
     2041 ttgcctcact cagttcggta tctaaattag attattctga ggctcttgat ggtatatcaa
     2101 acacattctc cattgttcca aaatcattag gaccaacaga cgaagaacgt gcagataact
     2161 tagtgtcaat tattgatggt tattttaaca aagaagctca tcacttaaat gtgaatgtgt
     2221 tgaatcgtga gatgctggaa gatgctatgg agcatccaga aaattaccca cagctaacca
     2281 ttcgtgtttc gggatatgct gtaaattttg ttcgtttaac acgagcacaa caacaagaag
     2341 taatttcgag aagtttcttc gaatcactct aattttataa atacactaca tgattaatgt
     2401 acattcggtt gagtcgttag gtacgtacga tggacctggt attcgcttgg tgcttttttt
     2461 acaaggatgt aatttcaagt gcctctattg tgctaatcca gacactatcg agcctaa
//
LOCUS       DAOSMY010000234         2512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_41441_length_2512_cov_3.782255, whole genome shotgun sequence.
ACCESSION   DAOSMY010000234 DAOSMY010000000
VERSION     DAOSMY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41441_length_2512_cov_3.782255"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLD76_05570"
     CDS             complement(<1..549)
                     /locus_tag="PLD76_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU27505.1"
                     /translation="MENKEVILITGSNGLIAKQLASSLASQYTIRLLTRKPTKPNEYC
                     WDIHQQFIDEKALENVSHIIHLAGAGIADKNWTNARKKEIVSSRVDSAKLLLTACIKQ
                     NSKLKSFISASAIGYYGQTSSEHIFTETDQHGTDFVSEVCVAWEKAADEFEKLGVAER
                     VIKLRTGIVMATEGGALPNILFP"
     gene            complement(554..2041)
                     /gene="crtI"
                     /locus_tag="PLD76_05575"
     CDS             complement(554..2041)
                     /gene="crtI"
                     /locus_tag="PLD76_05575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOU27506.1"
                     /translation="MKNQQVIIVGAGLGGLTTALRLVKKGYKVTILEKNTQAGGRLNR
                     IQKDGFTFDTGPSFFSMSYEFKKLAEECNITMPFEFVELDPLYSVHFAGDPRSYHLYK
                     DMKKLAEQFKDIEPDFEAKMAKYLQKSGALFHDTIDLVVKNNFDSIFEYIATLMRVNP
                     VHLPVLLRNFWQQVGLHFSSKEVKRIVSLVAFFLGRTPFDTMAIYTLLSYTEFKHDGY
                     YNVKGGMYKIVEGIVAELEKENVQFVYNTEIVNIQSEKIRVKSVIDQHGKEWLADIFV
                     INADAAVFRGTVMKRKKFSTKKLDKMNWTMGYLTIYLGIDCKLPQVNHHNYYLGTNYE
                     EYANNVMKNPDTLDKPYYYVNVLSKHNEECAPAGCESLFFVCPVPTLLQKPNWDDRDK
                     IVDSIIADFSNRIDKNIAPHIVSKTIFTPKEWETMINLHRGSGLGLSHTMFQIGGFRP
                     ANFDEKFENTFYVGASTIPGAGLPMAVISSELVCKRVYAFTEKNN"
     gene            complement(2062..>2512)
                     /locus_tag="PLD76_05580"
     CDS             complement(2062..>2512)
                     /locus_tag="PLD76_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27507.1"
                     /translation="IDALDNKKYKPADLHAYKAAFYGYKIAITPFKASYLGPKSIWHV
                     KKALEIEPENMFALLQYGNIYYYMPVTFGGSKETASQYYLKVEKWYEKHPQQRITNWN
                     YINVLVTLTNTYIALGKKDKATEYYNKIVTAEPTSSWIKQEIYPQLK"
BASE COUNT          707 a          470 c          414 g          921 t
ORIGIN      
        1 tggaaataat atgtttggta aggcaccgcc ttctgttgcc atcacaatgc ctgtgcgtag
       61 ttttattacc cgttctgcaa cacctagttt ctcaaactca tcggctgcct tttcccaagc
      121 tacacatact tcactcacaa aatcagtacc gtgttgatct gtttcagtaa aaatatgttc
      181 ggagcttgtt tgaccatagt aaccaattgc cgaagccgaa ataaatgatt tcaacttact
      241 gttctgtttg atacaagccg taagtagcag tttggcacta tctactctgc ttgatactat
      301 ctcttttttt cttgcattag tccaattttt gtcggcaata cctgctcccg ccaaatggat
      361 gatgtgagat acattctcaa gtgctttttc atcaatgaat tgttgatgga tgtcccagca
      421 gtattcattc ggttttgttg gtttgcgagt taagaggcga attgtatatt gactagccaa
      481 agaactcgct aattgcttag caattagccc attacttcca gttattaaaa ttacctcttt
      541 gttttccatg taactaatta tttttttccg taaaggcata tacgcgttta catactaatt
      601 ctgaacttat tacagccatg ggcaatcctg ctcctggtat ggttgaagca cctacataaa
      661 aggtgttttc aaatttttcg tcaaaattgg caggtctaaa ccctcctatt tgaaacatcg
      721 tgtgcgacaa tcccaatccg ctaccacgat gaagattaat cattgtttcc cattctttag
      781 gcgtaaaaat agttttagac actatatgtg gagcaatgtt tttgtcaata cgattagaaa
      841 agtctgcaat aatactatct acaattttat cgcggtcgtc ccaatttggt ttttgcaata
      901 gggtaggtac tggacataca aagaataaac tttcgcaacc tgcaggagca cattcttcgt
      961 tgtgcttgga tagtacgttt acatagtaat aaggcttatc taatgtatct ggatttttca
     1021 tcacattgtt ggcatattct tcgtaattag tgccgaggta ataattatga tgattaacct
     1081 gaggaagttt gcaatcaata cctaaataaa ttgttagata gcccattgtc caattcattt
     1141 tatccagttt ttttgtgctg aattttttac gtttcatcac tgtgcctcta aatacagcag
     1201 catctgcatt tatgacaaaa atatcggcaa gccattcttt gccatgttga tcaattacgc
     1261 tttttacacg tattttttca gattggatat tcacaatctc ggtattgtac acaaactgta
     1321 cgttttcttt ttctaattca gcaacaatgc cttctacaat tttatacata cctcctttta
     1381 cgttgtaata gccatcgtgt ttaaattcag tatatgataa tagggtgtaa attgccatgg
     1441 tatcaaaagg agttctgccc aaaaagaatg ctacaagcga tacaatgcgt ttaacttctt
     1501 tagatgaaaa atgtaagcct acttgttgcc aaaagttacg tagcaatacc ggtaagtgta
     1561 ctgggtttac acgcattaag gttgcaatat attcaaatat agagtcaaag ttgtttttta
     1621 ccaccaagtc gattgtgtca tgaaacaatg cacctgattt ttgcaaatat ttagccattt
     1681 tagcttcaaa atcaggttcg atatctttaa attgttcagc caattttttc atatctttgt
     1741 acaaatgata gctacgtggg tcgccagcaa agtgtacact atacaagggg tcgagttcaa
     1801 caaactcaaa tggcatcgtt atattacatt cttcagctaa ttttttaaat tcatacgaca
     1861 tactaaaaaa acttggacct gtgtcgaacg tgaaaccatc tttttggatg cgatttaatc
     1921 ttccaccggc ttgtgtgttt ttttctaata tggttacttt gtagcctttt tttactaaac
     1981 gtaatgctgt ggttaatccc cctaatccag cacctacaat gataacttgt tgatttttca
     2041 tataccaata ttctttgatt tttattttaa ttgcggataa atttcttgtt taatccaaga
     2101 tgatgttggc tctgccgtta ctatcttgtt gtaatactct gttgctttat ctttttttcc
     2161 tagagcaata tacgtgtttg ttaatgttac taatacattg atatagttcc agttagtaat
     2221 acgttgttgt ggatgttttt cgtaccattt ttctactttc aagtaatact ggctagctgt
     2281 ttcttttgat ccgccaaatg ttacaggcat ataatagtaa atatttccat attgcaataa
     2341 cgcaaacata ttttcgggtt caatttccaa tgcctttttt acatgccaaa tgctttttgg
     2401 tcctaaataa cttgctttaa aaggtgttat tgcaatttta tatccataaa atgctgcttt
     2461 ataagcatgt aaatcagcag gtttgtattt tttattatcc aatgcgtcaa tg
//
LOCUS       DAOSMY010000235         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_41860_length_2494_cov_2.838048, whole genome shotgun sequence.
ACCESSION   DAOSMY010000235 DAOSMY010000000
VERSION     DAOSMY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41860_length_2494_cov_2.838048"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /gene="porV"
                     /locus_tag="PLD76_05585"
     CDS             complement(<1..1099)
                     /gene="porV"
                     /locus_tag="PLD76_05585"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOU27508.1"
                     /translation="MKKFSIFLIAIFSLTNIFGQASLEGDVNVFNPITTGVPFLSIAP
                     DARGGAMGDVGAATSPDAYSQYWNPSKFAFIDGAGGVSLSYTPWLRKLVKDIDLAYLS
                     GYYKIADIQTVSASLRYFSLGEVMIRKDATSPAITAKPYDLAVDASYARILAENFSMG
                     VTLRFIYSDLLGGAPVEEAESLTPGAAVAADISAFYSSPVVFPEGEGFARGGLNISNI
                     GSKLSYGDDLNMAFLPTNMRLGGSLTYPLDPYNKLSVNLDLNKLLVPSRPVAPTQGDD
                     ESDADYALRESKYETSLSDYNNGDPIAGIFTSFSDAPGGFSEELQEIMWSLGLEYAYN
                     NQFFARAGYFNESANKGNRKYATIGAGFKLNV"
     gene            complement(1156..>2494)
                     /locus_tag="PLD76_05590"
     CDS             complement(1156..>2494)
                     /locus_tag="PLD76_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU27509.1"
                     /translation="SAGMNVLLNPYGGGIGLLTTTRTVYSFQNYNLSKQIYRYLLPED
                     PFNPMPLGEIMRRAKVGMGADENKLNFALLGDPMLSLSYPTNRVITTQINDAVLPDIA
                     TIKSLSLVTIKGYIENNEQNGILEEFNGFIYVTVYDKEETIHTLSNRGNVPFTYKDRP
                     NVIFSGKTTVVNGEFTILFMVPKDINYRYGDGRIVYYAVDETLNREANGYSEDLIVGG
                     SSDDISTNQEGPIVRMYLNTPHFLSGQTVNNSPVFYAFMNDDFGINTVGAGIGHDIVL
                     KLNNQTNYTYVLNDYYQATLDDYKSGYIRFPLNNLPAGSYELQFKVWNLQNISTTQTL
                     YFTVEDTAKPSIYSFYAYPNPASVSTNFVLEHDRPDVLATATFIVCDITGRKVWQSQT
                     VTILGDEPLQLTWDLTDSSGRKLPSGHYLMNVKIETPNEAFSTATQKIIIIGQ"
BASE COUNT          844 a          499 c          404 g          747 t
ORIGIN      
        1 acacattgag tttgaaccct gcaccaatag ttgcatattt acgatttcct ttgttggcac
       61 tttcgttaaa atatcctgca cgagcaaaaa actgattgtt gtaggcatat tcaagaccca
      121 atgaccacat aatttcttgc aattcttcgg agaaaccacc aggagcatct gagaatgagg
      181 taaatatacc tgcaatagga tccccattat tgtaatcact taaactagtc tcatatttac
      241 tctcacgcaa agcatagtca gcatcacttt catcatctcc ctgagtagga gcaaccggac
      301 gagaaggcac caataactta ttaaggtcaa gattaacaga taatttgttg tatgggtcaa
      361 gtggataggt caacgaacca cctaaacgca tattggtagg caagaaagcc atatttaaat
      421 cgtcaccata cgaaagctta gagccaatat tggaaatatt caaaccacca cgagcaaaac
      481 cttctccctc tgggaatacc acgggagagc tataaaaagc agaaatatca gctgctacag
      541 cagcacctgg agtcaaagat tctgcttctt ccacaggagc cccacctaac aaatcggaat
      601 agataaaacg taacgttaca cccattgaaa aattctctgc caaaatacga gcataacttg
      661 cgtctacagc taaatcatac ggtttggcag taattgcagg agaagtagca tctttacgaa
      721 tcataacttc acccaaagag aagtagcgaa gcgaggcaga aacagtttga atgtcagcaa
      781 ttttataata gccagacaaa taggctaaat caatatcttt tactaattta cgcaaccaag
      841 gagtataaga taaagacaca ccccctgcac catcaataaa agcaaatttt gatggattcc
      901 aatactgcga ataagcatct ggcgaagtgg ctgcacccac atcccccatt gctcctcctc
      961 gtgcatcggg tgctatggat agaaagggta ctcctgtcgt aatcggatta aaaacattca
     1021 catcaccctc aagtgatgct tgaccaaaaa tgttagtcaa agagaaaatg gcaattaaaa
     1081 atatactaaa ttttttcatt ctaaataatg tattgtgtgt ttagcgaatg caaatataac
     1141 gctaaaattt ttaatttatt gtccaataat gataatcttt tgagtagccg tagaaaatgc
     1201 ttcattaggc gtttctattt tcacattcat caaataatgt cctgaaggta gttttcttcc
     1261 tgaactatca gttaaatccc acgtcaattg taaaggttca tctcctaata tagtaacagt
     1321 ttgcgattgc catactttac gaccagttat atcacataca ataaatgtag cagtagctaa
     1381 cacatcaggt ctatcatgct caagcacaaa atttgtactc acagaagcag gatttgggta
     1441 ggcataaaac gaataaatag atggttttgc tgtatcttca accgtaaaat acaaggtttg
     1501 tgtagtagat atgttttgca aattccacac tttaaattgt aattcgtaac tgccagcagg
     1561 taaattattg agtggaaaac gaatataccc acttttataa tcatctaatg tggcttgata
     1621 ataatcgttt agcacatagg tataatttgt ttgattatta agtttgagca caatatcatg
     1681 accgatacct gctcctactg tattaatacc aaaatcgtca ttcataaaag cgtaaaatac
     1741 gggactattg ttaaccgttt gccccgacaa aaaatgcggt gtattaagat acattcggac
     1801 aataggtcct tcttgatttg ttgagatatc atcacttgaa ccacctacaa ttaaatcttc
     1861 cgaataacca tttgcttctc gatttagagt ttcatcaaca gcataataca ctatacgacc
     1921 atcaccatag cgataattga tatctttagg caccataaac aatatagtaa attcgccatt
     1981 tactactgtt gtctttccag aaaaaataac attaggtcta tctttatacg taaatggcac
     2041 atttcctctg ttggataaag tgtgaatggt ttcttcttta tcatatacag ttacataaat
     2101 aaaaccatta aactcttcta aaattccatt ttgctcatta ttttcaatgt atccttttat
     2161 tgtaactaag ctcagtgatt ttatagtagc tatatctggc aaaacagcat catttatttg
     2221 ggtagttata accctatttg ttggataaga taaagatagc ataggatcgc ccagtagtgc
     2281 aaaattcaat ttattttcat ccgcgcccat accaaccttt gctctacgca taatttcacc
     2341 taatggcatt ggattaaaag gatcctctgg caaaagatat cgataaattt gcttacttaa
     2401 attataattt tgaaatgaat ataccgtacg cgttgtagtt aataagccta tacctccccc
     2461 atatggatta agcaatacat tcattcctgc agat
//
LOCUS       DAOSMY010000236         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_42027_length_2487_cov_3.422697, whole genome shotgun sequence.
ACCESSION   DAOSMY010000236 DAOSMY010000000
VERSION     DAOSMY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42027_length_2487_cov_3.422697"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1164..>2487
                     /locus_tag="PLD76_05595"
     CDS             1164..>2487
                     /locus_tag="PLD76_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOU27510.1"
                     /translation="MQLQKIKVGELKLSDIVYDIEHGHLRIPRFQRDFVWERSKVIKL
                     LDSIYKEYPIGSFFIWEADKKYNLFYRNIGELNLPQPDSHTSIRYILDGQQRATSLYA
                     TIKGLEVNGTDYSQICFDFDKEVFVVRYREHENCAPLRNILDENNHLQIYNQLSDARK
                     KTFEKCRNIFTTYPISVIIVREKDLDEASEIFERINQGGKRLSIFDLVVASTWGDDFD
                     LKEKYNQLRDFLVDKGFGKIPPEVVTHTASLTIKGYCRNSYQLQLTKEELKENWEEIS
                     NSIKLSIDFLTNNLGAKIYDFVPYPSIISLLAYLYFKSPGRSLTKQMTEKVHEWFWKA
                     ALSERYASSRETRMEEDRRLIFDKLLEGEDVKISFPINLDEEKIARSKISTKSALRNA
                     FFCMLALRHPRHFKTNNIVTLDYSLCSNFNSPEKHHIFPRHFLRKNKLD"
BASE COUNT          823 a          442 c          419 g          803 t
ORIGIN      
        1 gagcgaattt acagatgaaa gacatgttac cgaatccctg tcccccagcc aagtaaaact
       61 aaccgaagca aggactaaag aagctattct taaatcaatc ttttctttgg tttcgaactc
      121 ctcgggaatt tcagccactt cattattaga tttctcaatt aagtagagcg gttttgctcg
      181 tcaatacaaa gtttttttgt ctttgataga aagtttcgaa cccaatgttg ccaatatttg
      241 tgttttatct ttcaaagtac ctctttcgaa ccaatgtcga gcgtagcaga caaacgtaaa
      301 tgtcctttct gacagttcca accacttgtt tatacgaata tctgtattgt ttacacttgc
      361 cgataagatc tctttttaag ctaccaactc tcttctttgc tttaagtatt cttcttcact
      421 aataaagtta tcatttatgt tctagggcga gattttgagt tttaacaagc tatctatgcg
      481 tttaacacaa tcatcatatg tctcttgctg gttctttcta aagtgaattg tatgttgagt
      541 acaacttttt tatattatta ataacgtttc tacttgtcat ggtttacatg gtttactaag
      601 ctatccctaa caaatatttc aaactctctt ttaggtattc tatagacctt accaagtttt
      661 attgccctta gcttcccact tttaatgtga ctcagaatag tttgccaatg cacctgtaga
      721 agttctgcga actactcaac tgtgtaatat tcgtattttc tgggtttgtt atcattggag
      781 ttcaatataa gcaattataa acgagagtat caatagctaa cgttctataa atactagaat
      841 atattgcaat ctgatagttt tgtgcggtat gtgcgtacac taaatgtata taatatatgt
      901 gtattaaatg gttaataaaa atggctctat gaagataaat agtgccacaa tcgattttga
      961 tagcgaaaca aataagaaat ttatactaaa aactcacctg gtgacacccc acaacgccaa
     1021 aaacataccc aaaacaaagt ttagatagtt tccagtgata taatgcttta tatgtttagt
     1081 tttcttaaaa aagactagaa gatgcacaaa ttagagggta ggattaatat taaaattaag
     1141 agtatttgga aggttaatat gttatgcaac tacaaaaaat taaagtaggt gaattaaaac
     1201 tttctgatat tgtatatgat attgaacacg gacatttacg catacctcgt tttcaaagag
     1261 attttgtttg ggaacgctcc aaagtaataa agcttctgga ttcaatttac aaagagtacc
     1321 ctattggaag tttctttata tgggaagcag ataaaaaata taaccttttt tatcggaaca
     1381 ttggtgagtt aaatctacca caacctgact cacatacatc tattcgatat atactagatg
     1441 ggcagcaaag agcaacatct ctttatgcca caattaaagg acttgaggtt aacggaacgg
     1501 actattctca aatttgtttt gactttgaca aggaagtgtt tgtcgtgaga tacagggaac
     1561 atgaaaattg tgcacccctt agaaatatcc ttgacgaaaa taaccatctt caaatatata
     1621 atcaactgag cgatgcacga aaaaagactt ttgaaaaatg tcgaaacata tttactacgt
     1681 acccaatctc agtcatcatc gttcgtgaga aagatttgga tgaagctagt gaaatttttg
     1741 agcgaattaa tcaaggaggt aaaagattat cgatctttga tcttgttgtc gctagtacct
     1801 ggggcgatga ttttgattta aaagagaagt acaaccaatt gcgtgacttt cttgttgata
     1861 aaggctttgg taaaattcct ccagaggttg ttacgcacac tgcctcactc accattaagg
     1921 gttactgtcg aaattcatat caacttcagt taactaagga ggagttaaaa gaaaattggg
     1981 aagaaatttc aaattccata aaactgtcca tcgactttct aactaataac cttggggcaa
     2041 agatatatga ctttgtgcca tatccctcca taatctcgct actagcgtat ttatatttca
     2101 agtcacctgg gcgttcttta acaaaacaaa tgacagaaaa ggttcatgaa tggttttgga
     2161 aagccgcttt aagcgaacgt tacgcctcat caagagaaac acgtatggaa gaggataggc
     2221 gtttaatatt tgataagctt cttgaagggg aggatgttaa gatttcattc cctattaacc
     2281 tagacgaaga aaaaatagct agaagcaaaa tcagtacaaa atcagctcta cgcaatgcct
     2341 ttttctgtat gttagctctc cgacacccac gacattttaa aaccaataac atagttaccc
     2401 ttgactactc actttgctcc aatttcaaca gtcctgaaaa acatcacatc ttcccaagac
     2461 attttttgcg aaagaataaa ttagatg
//
LOCUS       DAOSMY010000237         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_42059_length_2486_cov_2.769231, whole genome shotgun sequence.
ACCESSION   DAOSMY010000237 DAOSMY010000000
VERSION     DAOSMY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42059_length_2486_cov_2.769231"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PLD76_05600"
     CDS             complement(<1..1489)
                     /locus_tag="PLD76_05600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU27511.1"
                     /translation="MNKNKLSANQLFWILNIAWMLLNLLQASVTELANDEAYYVVFAK
                     HLDWGYYDHPPMIALFIYLGQWLSGELGVRLLTVLSQPIYIWLLWKLIAPATVEKKDV
                     WLFFIIALAMPILHIYGFVAVPDAPLMLFSALFLYFFVQYLQHTSWYYIPFMALAIAA
                     IGYSKYTGVLVVLFACTAYPKVFLRKGIYITATLSLLLMLPHLYWQYQHEWVSFQYHL
                     VGRNQDFNAFYIAEYVGNFIAVFNPFILPLVVWIGFKNRHQDELTLLILKRIALFFFL
                     FFFASCIRGHVQPQWFIFVTFSVIALVWQWARNHEKRYRYIRTVSIISIVILIGFRIV
                     AATNPFGMKAEVFNNKTSYTQIANATGNLPVIFMGKYTMAAKYSYYTGLDAHASPNIH
                     FRTSQYQLWDFDSNLLEKPVAIHVGGDYPNATHIQTANGQFSFVIDSCYMPIRKVQIQ
                     WLNPYNTIASKREEKNMTLSIHNPYPYDIVIDNEKIQLHYIIRKPW"
     gene            complement(1532..>2486)
                     /locus_tag="PLD76_05605"
     CDS             complement(1532..>2486)
                     /locus_tag="PLD76_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU27512.1"
                     /translation="TKLHSTDTLVITAKGNIVRSRGGVTAFYTAQLNDLSSNAQYAYQ
                     VLSGEKQTEWYQFKTTSTNSPFSFMYIGDVQDRDTSNTSQQAFQYIYQHFTEADFWAF
                     GGDQIERPMNSYWEAFMNDLDGIRQSTPIIAIAGNYEYLKYAIRKIDDRFTYTFPYFI
                     NNHNNNHVFTFVYGNVRFILLDSNRESWYIWQQRNYLKEVLKNNSSLFTVVMLHHPVL
                     SNKSSWNNASIRLMMEPILSQYPVDLVLQGHEHGYARNSKKDKSGNSKLPIYVTSHLS
                     KKFYPSFKATKITDAIIPNTALFQHIKVDNNILTLYSYTLQ"
BASE COUNT          795 a          418 c          475 g          798 t
ORIGIN      
        1 accaaggttt tcggataatg taatgaagtt gtattttttc gttatctatc actatatcat
       61 aaggatatgg attgtgtatt gatagagtca tattcttttc ttcccttttt gatgctatcg
      121 tattgtaggg gttaagccac tggatttgta ccttgcggat aggcatatag caactatcta
      181 taacaaaact aaattgacca ttagcagttt gtatatgtgt agcattaggg taatcgccac
      241 ctacgtgaat tgctactggt ttttctagta agttgctatc aaaatcccat agttgatatt
      301 ggctggttct aaaatggata ttgggtgaag catgtgcatc taaacctgta taataactgt
      361 atttagctgc catagtgtat ttacccataa atattactgg taaatttcct gtagcattgg
      421 caatttgagt gtagcttgtt ttattattaa acacctcagc tttcatcccg aaaggattag
      481 tggctgcaac aatgcggaaa cctatcagta taacaatgga aataatacta actgtacgta
      541 tatagcgata ccttttttca tggtttctag cccattgcca aaccaatgca ataacactga
      601 aggttacaaa aataaaccat tgcggttgca cgtgtccgcg tatgcatgag gcaaaaaaga
      661 ataaaaagaa aaataaagct atgcgtttta ggattagcag tgtaagctcg tcttgatgtc
      721 ggtttttgaa accaatccat actaccaaag gcagaataaa tggattgaat acagcaataa
      781 aattacctac gtattctgct atgtaaaagg cattaaaatc ttgatttctg cctacaagat
      841 gatactgaaa cgatacccat tcgtgttgat attgccaata taagtgaggt aacatcaaca
      901 ataaggataa agtggctgta atgtaaattc ccttgcgtaa aaatacttta ggatacgctg
      961 tacaagcaaa tagtactact agcacacctg tatatttact ataaccaatg gcggctattg
     1021 ccaatgccat gaatggaata tagtaccaag atgtatgttg cagatactgc acaaagaagt
     1081 ataaaaatag tgctgaaaaa agcatcagag gtgcatcggg aacagctaca aacccataaa
     1141 tatgcaatat gggcatagcc aatgcgatga tgaaaaataa ccaaacatct tttttttcta
     1201 cggtggcagg tgctatcagt ttccatagca accatatata gattggttgg gatagtaccg
     1261 taagcagacg tacacctaac tcgccagata accattgccc aagataaata aacaaggcaa
     1321 tcataggtgg atgatcataa taaccccaat cgagatgctt ggcaaatact acgtaatatg
     1381 cttcatcatt ggctagttct gttacactgg cttgcaacag atttaacaac atccatgcta
     1441 tgttcaaaat ccaaaatagt tgattggctg acagtttatt cttattcata gtaaggggtt
     1501 attttacaat acttacacta tctacttttt ctcattgtaa ggtgtatgaa tataaagtta
     1561 agatgttatt gtcaactttt atatgctgaa ataaggctgt gtttggaata attgcatcgg
     1621 taatttttgt tgctttaaag ctgggataaa acttctttga cagatgagag gttacgtaaa
     1681 taggtagttt actattgcca cttttgtctt tttttgaatt acgagcatat ccatgttcgt
     1741 gtccttgtag taccaaatca acaggatatt gtgataaaat aggttccatc atcaaacgga
     1801 tagatgcatt gttccagcta cttttgtttg ataatacagg gtggtgtagc attaccactg
     1861 taaatagaga tgagttgttt tttaaaactt cttttaggta atttcgttgt tgccaaatgt
     1921 accaagattc acgatttgag tcgagcaaga taaaccgtac attaccatat acaaaagtaa
     1981 atacatggtt gttgttatgg ttattgataa aataagggaa tgtataagta aacctatcat
     2041 caattttgcg gatggcatat ttaaggtatt cgtaattgcc agcaatagct ataattggag
     2101 tagattgcct tatgccatca aggtcgttca taaaagcctc ccaatagcta ttcattggac
     2161 gctcaatttg gtcgccacca aaagcccaaa aatcagcctc agtaaaatgt tgatatatgt
     2221 attgaaaagc ctgttggctg gtattactgg tatctctgtc ttgcacatca ccgatgtaca
     2281 taaaagagaa cggactatta gttgatgttg ttttaaactg gtaccattca gtttgttttt
     2341 cgcccgaaag tacttgatat gcgtattgtg cattacttga taaatcgttc aattgggcag
     2401 tataaaaggc tgttactcct ccacgcgagc gtacaatgtt tcctttggca gtaattacaa
     2461 gcgtgtcagt agaatgtaat ttggtt
//
LOCUS       DAOSMY010000238         2482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_42138_length_2482_cov_2.344046, whole genome shotgun sequence.
ACCESSION   DAOSMY010000238 DAOSMY010000000
VERSION     DAOSMY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42138_length_2482_cov_2.344046"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PLD76_05610"
     CDS             <1..1594
                     /locus_tag="PLD76_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOU27513.1"
                     /translation="EMLEVVATIQDVSCFGGSDGYIWLTISKGVGPYPVEWTYPDGNI
                     VLNQNITNLKAGLYKAVVTDARGHRFEREYNISQPDLLEVENLISSRLDIPCFGEKTG
                     RLDIRVTGGTTPYSIQWTGPDVPTVVPDSLSVQSLGAGTYTVHIKDDNGCRVQSTQTI
                     NQPTRLQVQAVVTNNTCYDQANGSIDLIVSGGVPSYTFDWTGIGVVPRVEDQANLATG
                     DDYAVRVWDSNDCFVDRKFSISSPTELLIDVEQVNVSCYGKRDGRLHARVSGGSYPYQ
                     HRWTYENGTVVQDSIILALYPGKYTYTLTDASGCTVVSDEYEITQPGPLQVSISGSSM
                     LCQGVDDGELIATASGGTGTGYSFTWTRLSPNTPFGVGPQLTNLGPGWYRVQVTDKNQ
                     CTATDEKEIKYSPIMSIQLVEKQNVLVTGENTGIIELDVQGGTPALTYSWSGGNLPTP
                     SPNSLRVENLIAGRYYFTVEDGLGCSIDTTITITQPEIIDVTAVTKNIACHGESNGYI
                     WPIQGYRKKMCNMHVLVGRTNN"
     gene            1758..>2482
                     /locus_tag="PLD76_05615"
     CDS             1758..>2482
                     /locus_tag="PLD76_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOU27514.1"
                     /translation="MYKGFTDDNDNLEYLMNSDESSDYNINNIINATKKPTLDNLLQF
                     VKQSIEVLSSDKDILKHFTPELNWLKDHLEVWQLHEWRVAIIGITSSGKSTLINALLN
                     DNLLPEKVKPSTNCFVLCRYGKKDKAVIHFKDGKTEEIDNVNKIKERIKILTDEETNP
                     GNNKCVKEIELYWPKFILGEGVVLIDTPGLDAYKLERHEIMTMQMLHDLVDAVIFLTT
                     AKANTDGVIAKYLDTIANQKKPLL"
BASE COUNT          831 a          398 c          538 g          715 t
ORIGIN      
        1 tgaaatgtta gaagttgtgg caactattca agatgtaagt tgttttgggg gtagcgatgg
       61 ctatatttgg ttaactattt ctaagggtgt aggaccttac ccagtagaat ggacatatcc
      121 agatggaaac attgtgttga atcaaaatat tactaacctg aaggctggct tgtacaaagc
      181 tgtagtaaca gacgcaagag gacatcgttt tgagcgtgag tacaatattt cgcaacccga
      241 tttgctcgaa gtggagaatt taatttcttc tcgtttagac attccttgtt ttggagaaaa
      301 aacaggtagg ttagatatca gggttacggg tggaacaaca ccatactcca tacagtggac
      361 tggaccagat gtgcctacgg tggttcccga ttctttgagt gtacagtcct taggagcagg
      421 tacctatacc gtgcatatta aagatgataa tggttgtagg gtacaatcta cacaaacaat
      481 taaccaacct acacgtttgc aagtgcaagc tgtggtaacc aataacacat gttacgatca
      541 ggcaaatggt agtatagatt taattgtgtc aggtggtgtg ccatcatata catttgactg
      601 gactggtatt ggtgtagtac ctagagtcga agatcaagcc aacttggcaa ctggggacga
      661 ctatgctgta agagtgtggg atagcaatga ttgttttgtg gataggaaat tttcaatttc
      721 ttctcctacc gaattactga ttgatgttga gcaagttaat gtgtcatgtt atggtaaacg
      781 tgatggacgt ttgcatgctc gagtaagtgg tggttcgtat ccatatcaac atagatggac
      841 gtatgaaaac ggtactgtgg tacaagattc aattattcta gctttgtatc ctggaaaata
      901 tacctacaca ttaactgatg cgtctggatg tacagtcgtg agtgatgaat atgagataac
      961 ccaacctgga ccattgcaag tatcaatttc tggttcaagt atgttgtgtc agggcgttga
     1021 tgatggtgaa ttgattgcta cggcaagcgg gggaactggt actggatata gctttacatg
     1081 gacaagatta agtcctaata ctccttttgg agtgggacct caattaacca acttgggacc
     1141 aggatggtat agggtacaag taacggataa aaatcaatgt acagccaccg atgaaaagga
     1201 aattaagtat tctcctatca tgagtattca attggttgaa aaacaaaatg tattggttac
     1261 aggcgaaaac acaggtatca ttgaattgga tgtacaagga ggaacacctg cacttacata
     1321 ttcgtggagc ggtggaaatt tacctactcc atcaccaaat agtttaaggg tagaaaattt
     1381 gatagctggt aggtactatt ttactgtaga agatggctta ggttgttcta ttgatacaac
     1441 tattaccatt acacaaccag aaataataga tgtaactgca gttactaaaa acatcgcttg
     1501 tcatggagaa agtaatggct acatatggcc aatacagggt taccgaaaaa aaatgtgcaa
     1561 catgcatgtt ctggtcggga ggacgaacaa ttgattcacg cgcttataaa ccatattttg
     1621 tgaatgcaga ttccaaaaaa gctgattgct tagtacaaaa aggaagaact tcaaccgctg
     1681 caacatactg cccaaaatgg caacaatggg agaagttgtt ttgttcaaaa taacattatt
     1741 taattgaaag tatacttatg tataaaggat ttacggatga taatgacaat ttagagtatt
     1801 tgatgaattc tgatgaatcg tcagactaca atattaataa tataattaat gctacaaaaa
     1861 agcccacact tgataattta ttacaatttg ttaagcaatc aatagaagtt ctatcctctg
     1921 ataaagatat tcttaaacac tttacccccg aattaaactg gcttaaagac catcttgaag
     1981 tctggcaatt acatgaatgg cgtgtagcaa ttattgggat tacgtcatca ggtaaatcaa
     2041 ctcttataaa tgcacttcta aatgataatc tacttcctga aaaagtaaag cccagcacaa
     2101 attgttttgt cctttgtcgc tatgggaaaa aggacaaagc tgtcattcat tttaaggatg
     2161 gaaaaacgga agaaattgat aacgttaata aaataaaaga aaggatcaaa atattaacag
     2221 atgaagaaac caatccaggg aacaataaat gcgtaaaaga aattgaactg tactggccta
     2281 aattcatttt gggcgaaggg gttgttctaa tagacactcc gggacttgat gcttataagc
     2341 ttgaaagaca tgaaataatg acaatgcaaa tgctgcatga tctagttgat gcggtcattt
     2401 ttctgactac ggctaaggct aataccgatg gagtgatagc aaaatattta gatacgattg
     2461 caaatcaaaa aaaaccgctg ct
//
LOCUS       DAOSMY010000239         2475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_42302_length_2475_cov_2.871074, whole genome shotgun sequence.
ACCESSION   DAOSMY010000239 DAOSMY010000000
VERSION     DAOSMY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42302_length_2475_cov_2.871074"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..363
                     /locus_tag="PLD76_05620"
     CDS             94..363
                     /locus_tag="PLD76_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27515.1"
                     /translation="MRKLSISTNCHFDLQKYENQSIASIASQYANTEEELDNLYEFGV
                     LKLAEYQNKLSTEEFDRFGFWYIKQDIIKKTTNAHNRVNHPTSKD"
     gene            843..2141
                     /locus_tag="PLD76_05625"
     CDS             843..2141
                     /locus_tag="PLD76_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOU27516.1"
                     /translation="MIESAPSYKQDDSIISLMVSLQLNGTLKAIQDEYLYWDKVKYKS
                     KDINPVDLWNAVKLHRKINSNAVTFGQYRFFYVVTDYLQRALHQFDIHIGGTLGSNIE
                     IVESDKTKYIISSIMEEAISSSQMEGANTTRKKAKEMIQKEQKPKNKSEQMIMNNFIT
                     MKHIVQHKNEDITPEKILYIHKLMSNKTLDEIEDEGRFRDNNEVHVVNHINSEIVHTP
                     PTNDELEKLINDLCFFFNNDSKDFIHPIIKGCIIHFMVGWIHPFTDGNGRTARALFYW
                     YMLKKGYWLTEYLSISRIIKDTKNQYEKAYLYSENDENDLSYFITYHVKTMEKAFDAL
                     KEYINRKQKEVFQAAKFMKIPLVNERMAQILKILNDDSDRILNIKEIENRFNISNYTA
                     RTDLKTLVDLGFLEVIQVNKKKQNFIKSKNFEDILHQQKI"
BASE COUNT          960 a          383 c          377 g          755 t
ORIGIN      
        1 ctgtttatgg ttatacattt tttagtatat tgacaccaat attatcaatt gtatttttaa
       61 tcggacgcat ttttcacacc taactataaa gctatgagaa aattatcaat ttccacaaat
      121 tgccattttg atttacagaa atatgaaaat caatccattg cttctattgc aagtcaatat
      181 gcaaatacag aagaagaatt ggataattta tacgaatttg gagttctgaa attagcagaa
      241 tatcaaaata aactttcaac agaagagttt gatagatttg gtttttggta cataaaacaa
      301 gatataataa aaaaaacaac gaacgcccat aatcgagtaa accaccccac cagtaaagac
      361 tgatggggag agcctctcac accaaaatca agcaatcttt agttaacacc tttacatata
      421 ttcataacca agcgtttgta aacattctaa agcaatgtta tttagtaata tgagcctaca
      481 acccatattc cctctaaaaa ctttcaactt tatacttttc tctttcaact atcaactatc
      541 aactatcaac tacaataaaa atctctcccc cttttgcaaa ttaaaaaaac tttagtagat
      601 ttacacaaac gttttttaga agtggatata cgccataact attgctacct taaaaaaata
      661 tattattcta actgtcaagt tattatgttt tttataatgc acatatatac gccatgctat
      721 ggcacatata cagatattgg gcaacacctt aatagacagt aaactaaaaa tatcaatgta
      781 aataatgcga gatttgatta aattctcgca ttatttagta tctttgttga aaaatttgaa
      841 atatgattga atctgctcct tcatataagc aagatgatag tataatatcc ctgatggtct
      901 ctttacagtt aaatggaact ttaaaggcta ttcaggatga atatttgtat tgggataaag
      961 ttaaatataa atcaaaggat ataaacccag tcgatttatg gaatgcggtt aaacttcacc
     1021 gcaaaataaa cagcaacgct gtgacatttg gtcaatatcg ttttttttat gtcgttacag
     1081 attacttgca gagagctttg caccagtttg atatacatat aggtggtaca ttaggaagta
     1141 atattgaaat tgtagaatct gacaaaacca aatacataat tagctctatt atggaggagg
     1201 cgatatctag tagccagatg gagggggcaa atactactag aaaaaaggct aaagagatga
     1261 ttcaaaagga acaaaagcct aaaaacaaat ctgagcaaat gattatgaat aatttcataa
     1321 caatgaagca tattgttcag cacaaaaacg aagatatcac acctgagaaa atactatata
     1381 ttcataaact aatgtcaaat aaaacattag atgaaattga agatgaaggc aggtttagag
     1441 ataataatga agttcatgtt gtaaatcaca taaatagcga aattgtacac acgcctccga
     1501 caaatgatga attagaaaaa ctgataaatg atttatgttt cttttttaat aatgattcta
     1561 aagactttat acatccaata ataaaaggat gcatcataca ttttatggtt gggtggatac
     1621 atccttttac agatggtaat ggacgtactg caagagcatt attttactgg tatatgctaa
     1681 aaaaagggta ttggcttaca gaatatttat caatatcaag gataattaaa gataccaaga
     1741 atcaatacga aaaagcttat ttatactcag aaaatgacga aaatgattta agttatttta
     1801 taacatatca cgtaaaaaca atggaaaagg ctttcgatgc tttaaaagaa tatataaaca
     1861 gaaagcaaaa agaggttttt caagctgcta aatttatgaa aataccatta gttaatgaac
     1921 gaatggctca gattcttaaa atacttaatg atgattcaga tagaatttta aacataaaag
     1981 aaatagaaaa tagatttaat atttcaaatt atacagctag aactgattta aaaacacttg
     2041 tagatttagg atttttagaa gttattcaag taaataagaa aaaacaaaat tttataaaat
     2101 caaaaaactt tgaagatatt ctacatcagc agaaaatata aaaaaaggcg aacgcccaat
     2161 cgagtagact gccctaccag taaagactga tggggagagc cctcctccct acccctcgtt
     2221 ttcacaacga ggggtttatg gtttgtcgtt tgtaacgaca ataattcact aaaccaagat
     2281 agctggttaa ttccgtgagt tccaattagg taataagaac tttaaaaagg attacatacc
     2341 cctatttact aaaacgacaa aagtccaaac tattttggac ttttgtcgtt tttttgcgga
     2401 gaagatgaga ttcgaactca cgatacgctt ttgacgtata cacgctttcc aggcgtgcct
     2461 cttcaaccac tcgag
//
LOCUS       DAOSMY010000240         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_43255_length_2433_cov_3.818335, whole genome shotgun sequence.
ACCESSION   DAOSMY010000240 DAOSMY010000000
VERSION     DAOSMY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43255_length_2433_cov_3.818335"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..976)
                     /locus_tag="PLD76_05630"
     CDS             complement(68..976)
                     /locus_tag="PLD76_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU27517.1"
                     /translation="MKPTLYVMAAGMGSRYGGLKQMDGLGPSGETIIDYSIYDAIRAG
                     FGKVVFVIRKAFEADFRKIVISKFENKIPVEVVFQEVDKLPEGFSVHPERVKPWGTNH
                     AILMGKDVVKEPFAVINGDDFYGKESFEVLGKALQQMEGKSAQYCMVGFPVKNTLSES
                     GSVSRGVCQTDNNNNLVSVVEHTKIEAQNGRIVNLNDLGQELVVLDPNTPVSMNMWGF
                     TPDYFEFSEAYFKEFLKENEKSLKAEFYIPSMVDYLIKNNIATVKVLDTPSKWFGVTY
                     ADDKATVLMKLNELIHKGVYPAKLWS"
     gene            complement(1058..>2433)
                     /locus_tag="PLD76_05635"
     CDS             complement(1058..>2433)
                     /locus_tag="PLD76_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOU27518.1"
                     /translation="QQRYSKPGFPITKARLFVYTMQFRKHLEMKLPDEGDNYITAVAW
                     SSNPELMAINVLNREQNERKTIFYNPLSGVARMANQENASPAFSPLAARFLQFLPDNS
                     FTLLSEKTGYTQLYHYANNGILIRQLTNGKMAITDVYGYDTIGKQFFYQSFTKGLLNK
                     GIFAVNLQGKIRTLFDEEGVHTAYFSPQFSHFVHQYSNVNTPPYYTLYDVKMKKLGRV
                     FDNAAMADKMMKRVFPTKELLPMGWMVKPSDFDERKVYPVVVVLQETLNQWDISYAQD
                     IANQGYIVCSFSPTMYRYAGNEQEHLAVWHSYLETLFQYPFTNKNSIALMGVGAYAHT
                     AIELLQKDTTVKVAIALNPITDYAHYLSVLSEGWMGLPQKNFMAYQQAELSSLANYRG
                     KLLLMHNVDNTYVHIQHTWLLVDALIKANQQFDMQLYPSIYYDNVTQTLYLHSFERIM
                     SFLNKNM"
BASE COUNT          769 a          466 c          372 g          826 t
ORIGIN      
        1 caagaaacaa gcaatacaag gatgaaccca ataagataat ttcttttttt catggtttat
       61 gggagtgtta gctccatagt tttgctggat atactccctt atgaatcaat tcatttagtt
      121 tcattaatac cgttgcctta tcatcagcat acgttactcc aaaccattta gacggtgtat
      181 ctaacacttt tacggtagct atattatttt taataaggta atcaaccata cttggaatgt
      241 aaaattcagc ttttaaactt ttttcatttt cttttaaaaa ttctttgaaa taagcttctg
      301 aaaactcaaa ataatcaggt gtaaatcccc acatattcat ggatacaggc gtattgggat
      361 ctaatacgac tagttcttgt ccaaggtcat tcaaattaac aattctgcca ttttgtgctt
      421 caattttggt atgctcaact accgacacca aattattatt gttatctgtt tggcacacac
      481 cacgtgatac tgaaccactt tcggacaagg tatttttaac aggaaatccc accatgcagt
      541 attgagccga ttttccttcc atttgttgta aggcttttcc taatacttca aatgattctt
      601 taccataaaa atcatctcca ttaataacag caaaaggttc ttttaccaca tctttgccca
      661 tcaaaatagc atgattggtt ccccatggtt ttactcgttc gggatgaaca ctaaaacctt
      721 caggcaattt atctacttct tgaaaaacaa cctctacagg gattttgttc tcaaactttg
      781 aaataacgat ttttctaaaa tcagcctcaa aggctttgcg aataacaaat accactttgc
      841 caaaaccagc tctaatggca tcgtaaatag aataatcaat aattgtttct ccgcttggac
      901 ctaagccatc catttgcttt aatccgccat atcggctccc catacccgct gccattacat
      961 acaatgtagg tttcatcttg tttgctgtgt taatatattc tactaataaa atttgttcga
     1021 caaaagtagc aaaaaaagct tacgttttga ttgctattta catgttttta tttaaaaaag
     1081 acataattcg ttcaaaacta tgcagatata acgtttgtgt tacattatca taatagattg
     1141 atggatataa ttgcatatca aattgttgat ttgccttgat caaagcatct actaacaacc
     1201 aagtgtgttg tatatgcaca tacgtattat caacattgtg catgagtaat aattttccac
     1261 gataattagc caaagagctt agctctgctt gttggtaagc cataaaattc ttttgaggca
     1321 aacccatcca accttcactc aaaactgaca aataatgagc ataatcggta attggattca
     1381 gggcaatagc aacttttaca gtagtatctt tttgcaatag ctcaatagct gtatgggcat
     1441 atgcaccaac ccccatcagg gcaatgctat ttttattagt aaatgggtat tgaaaaagtg
     1501 tttctagata cgaatgccat actgctagat gttcctgctc attaccagca tatctataca
     1561 tggtaggact aaaagaacag actatatatc cctgatttgc aatgtcttgt gcatacgaaa
     1621 tatcccactg attaagggtt tcttgtagta caacaacaac tggatagacc tttcgttcat
     1681 caaaatcaga aggttttacc atccacccca ttggcaatag ttctttagta ggaaacacac
     1741 gtttcatcat cttgtctgcc atcgctgcat tatcaaatac acgacctagt tttttcattt
     1801 taacatcata caatgtatag tatggaggag tattcacatt actatattga tgtacaaaat
     1861 gtgaaaactg tggtgaaaaa taagcggtat gtacgccttc ttcatcaaac aaggttcgta
     1921 ttttcccttg taaattcaca gcaaatattc ctttgtttaa caatccttta gtgaatgatt
     1981 gataaaaaaa ttgtttccct attgtgtcat atccatacac atcagtaata gccatcttac
     2041 catttgtaag ttgacgtata agtataccat tgtttgcata atggtaaagc tgtgtatatc
     2101 ctgttttttc acttaataaa gtaaaagaat tatcgggtaa aaactgtaaa aaccgtgctg
     2161 ccaaaggaga aaaagctgga gaggcgtttt cttggtttgc cattctagct acacctgaca
     2221 atggattata aaaaattgtt tttcgttcat tctgctctct atttaaaacg ttaattgcca
     2281 ttaattctgg attagacgac catgctactg ctgtaatata attatctcct tcatcaggta
     2341 atttcatctc aagatgctta cgaaattgca tggtataaac aaataatctg gcttttgtta
     2401 ttgggaagcc tggttttgaa tacctttgct gaa
//
LOCUS       DAOSMY010000241         2400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44100_length_2400_cov_3.681450, whole genome shotgun sequence.
ACCESSION   DAOSMY010000241 DAOSMY010000000
VERSION     DAOSMY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44100_length_2400_cov_3.681450"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLD76_05640"
     CDS             complement(<1..433)
                     /locus_tag="PLD76_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOU27519.1"
                     /translation="MSDEYTINENEDKELDNLAEAETEAFAASSGYKVPTVTTEATKH
                     HLTGMYQKWFLDYASYVILERAVPHLNDGFKPVQRRILHSMKLLDDGRYNKVANIVGH
                     TMQFHPHGDASIGDALVQLGQKDILIDCQGNWGNTLTGDRAA"
     gene            740..2290
                     /locus_tag="PLD76_05645"
     CDS             740..2290
                     /locus_tag="PLD76_05645"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU27520.1"
                     /translation="MASQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVEL
                     KNNIKKYWWDSMVLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDLMAKYDDKINKEIEKAAKKFGDAFDATLFAETNPRIQENKAKKEEIHTRFAK
                     ALNDGDLNELKQIIVDYDVVCPISGTKNWTDVRQFNLMFSTEMGSTADGAMKIYLRPE
                     TAQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMKWFEHWKQFRLRWHKALGLGDHKYRFHDHDKLAHYANAATDIEFELPFGFKEIE
                     GIHSRTDFDLDSHEKYSGKNIKYFDPELNQSYTPYVVETSIGLDRTFLSIMATAYCEE
                     TLEGNDTRVVLKLPPALAPIKVCIMPLVKKDGLPEKAREIMDNLKFEFKCSYDEKDSI
                     GKRYRRQDAIGTPFCLTIDHQTLEDNCVTIRHRDSMQQERVAIDSLETIVGKAVSMKK
                     MLQSIVQD"
BASE COUNT          794 a          454 c          469 g          683 t
ORIGIN      
        1 ctgctgccct atcgccagta agcgtattcc cccagtttcc ctgacaatcg attaatatat
       61 ctttttgtcc caattgcacc agtgcatcac caatagaagc atccccgtga gggtgaaact
      121 gcatggtatg ccccacaatg tttgcgacct tattgtagcg cccatcgtcg agcaatttca
      181 ttgagtgcaa aatacggcgt tgaacaggtt taaagccatc atttaaatgc ggtacagcac
      241 gttcgagaat tacatacgag gcataatcca aaaaccattt ttggtacatg cccgttagat
      301 gatgcttggt agcctcagta gtaacagtag gtactttgta gccagaagat gctgcaaaag
      361 cttcggtttc tgcttctgct aggttatcta actccttgtc ttcgttttcg tttatggtat
      421 attcgtcgct catacgtaaa taatggtgca gaaaaatgcg atagtcaatt ttaggaacat
      481 aaaaaatgag tccgcaaaaa gattggctgt aaaattacaa ttttttttga taccaatgaa
      541 ttaaaaaaaa cacgaagttt gaacattttt tgtcaaaaag ttgtttttcc tgttttttga
      601 ttccataaaa caaggttaag caatgcaaat aaacgataat ttagccagat gccttccaaa
      661 attcaaaaaa aagcagtagt tttgtctctc attttcaata gatagaaaaa atatcgttta
      721 attgaactaa ttttatagta tggcgtcaca agaagatgtt tttaagaaat taattgcaca
      781 ttgcaaagag tatggttttg tatttccatc gagcgaaatt tacgatggct tgagtgctgt
      841 gtacgactat ggtcaaaacg gcgtagagtt aaaaaacaac ataaaaaaat actggtggga
      901 tagcatggtg ttgctacacg aaaacgtagt gggtattgat gccgctattt ttatgcaccc
      961 aaccacatgg aaagcttcgg gacacgtaga tgcttttaac gatccattaa tcgacaacaa
     1021 agactcgaaa aaacgctacc gtgccgatgt gttaatcgaa gatttgatgg caaaatatga
     1081 cgataaaatc aacaaagaaa ttgaaaaagc tgccaagaaa tttggcgacg catttgatgc
     1141 taccttgttt gccgaaacca atcctcgcat acaagaaaat aaagcaaaaa aagaagaaat
     1201 tcatacacgt tttgcaaaag ccttgaacga tggcgacctc aacgaattga aacaaattat
     1261 tgttgattac gatgttgttt gccctattag tggcactaaa aactggactg atgtgcgtca
     1321 gtttaacttg atgtttagca ccgaaatggg ttcgacggca gatggtgcaa tgaaaatata
     1381 tttacgcccc gaaacagcac aaggtatttt tgtaaactac ctaaacgtgc aaaaaacggg
     1441 tagaatgaaa attccttttg gtatagctca aataggtaaa gcattccgca acgaaattgt
     1501 agctcgtcag tttattttcc gtatgcgtga atttgaacaa atggaaatgc aattttttgt
     1561 gcgtccaggt gaggaaatga aatggtttga gcattggaaa caattccgtt tgcgttggca
     1621 caaagccctt gggttgggcg accataaata ccgttttcac gaccacgaca aattagcgca
     1681 ttacgccaat gctgctactg atattgaatt tgaattacca ttcggtttca aagaaattga
     1741 aggcatacac tctcgtacag attttgactt agatagccac gaaaaatatt cgggtaaaaa
     1801 catcaagtat ttcgatcctg aacttaacca atcgtacacg ccttatgtgg ttgaaacctc
     1861 aataggatta gatagaacct tcctttcgat aatggctact gcttattgcg aagaaacctt
     1921 agaaggcaat gatacgcgtg tggtactaaa attaccgcca gccttagctc ctatcaaggt
     1981 ttgtattatg cctttggtaa aaaaagatgg tctgcctgaa aaagcacgtg aaataatgga
     2041 caacctaaaa tttgagttca aatgttcgta tgacgagaaa gattctattg gtaaacgtta
     2101 tcgtcgtcaa gatgccatag gtacaccttt ctgccttacc atcgaccacc aaactttgga
     2161 agacaactgc gtaaccatac gtcatcgcga tagcatgcaa caagaacgtg ttgccattga
     2221 tagcctagaa actattgttg gcaaagctgt aagcatgaaa aaaatgctac aatcaatcgt
     2281 tcaagattaa acctttacta cctccttttt gtctaacaca taaaggaggt ttttttatac
     2341 cccatccgat taccaattaa tctggtcgtg aatgagagca aacaacgata aagaagcaaa
//
LOCUS       DAOSMY010000242         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44352_length_2390_cov_3.582869, whole genome shotgun sequence.
ACCESSION   DAOSMY010000242 DAOSMY010000000
VERSION     DAOSMY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44352_length_2390_cov_3.582869"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLD76_05650"
     CDS             <1..695
                     /locus_tag="PLD76_05650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213989.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOU27521.1"
                     /translation="IFYLHSRLLERAAKIIKNDVVARNMNDLPDSIKHLVKGGGSITA
                     LPIIETREGDVSAFIPTNVISITDGQIFLETSLFNAGIRPAINVGISVSRVGGNAQIK
                     AMKKIAGTLKLDQAQYRELEAFSKFGADLDAGTKLVLNKGSKNVEILKQKLHQPMPVE
                     KQIAIILCGVRGYLSHVPLNKVQEFENNLLEILEAKYKKDVLDILQKGDLTEDVLTKI
                     KDVAFTLCKKYE"
     gene            720..1598
                     /gene="atpG"
                     /locus_tag="PLD76_05655"
     CDS             720..1598
                     /gene="atpG"
                     /locus_tag="PLD76_05655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOU27522.1"
                     /translation="MASLKDIRARILSVRSTKKITSAMKMVSSSKLHKTETLCLNFLP
                     YKNALCESLANYLNSLDEKISVQVAEQRPIKRMAIICFSSNSGLCGTFNTNIAKLASE
                     KIDQYAYLGKENIYVYTIGKKITDYLQKLKYPIHAHYNNLLDNPSYDEVVKLADMLTE
                     LFISKQIDCVELLYNHYKNTACQTPTSEILLPLSSMQLTKTTKSQPLYIVEPSKEEFI
                     NALAPKLIRIKLYAAFLDSFTAEHAARTTAMQIATENAEEMIKTITQQYNKKRQEVIT
                     NELLDIVGGSEALRQD"
     gene            1615..>2390
                     /locus_tag="PLD76_05660"
     CDS             1615..>2390
                     /locus_tag="PLD76_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU27523.1"
                     /translation="MEITRVFDILENYKIIKPNQPIALAHKKEGNWIKYSIDEYIYQT
                     NLISAGLLYNGVKKGDTIAIISSNRAEYNIVDMAAMQIGAIPVPIYPTISESDYRYIL
                     NHAEITFIFLEGEELLRKIEHIVPEIPTLKGIYTFVNRGRFHYFEQLINLGKENLEID
                     KINAIKATITPNDVACMIYTSGTTGNPKGVMLSHQNITMQLKMLQNTPAKWSDTAFSF
                     LPICHAYEKMIVYLYQYLGMSVYYAESLATISENIKEIHPT"
BASE COUNT          916 a          393 c          386 g          695 t
ORIGIN      
        1 atatctttta tttacattca cgtttgttag aaagagcagc taaaatcatt aaaaatgatg
       61 ttgtggcacg taatatgaat gatttgccag attccatcaa acacttagta aaaggaggag
      121 gctctattac agccttacca attattgaaa cacgagaagg agatgtttct gcatttatcc
      181 ctaccaatgt catctccatt actgacggac agatattctt agaaacaagt ctgtttaatg
      241 caggtattcg tcctgcaatt aacgtaggta tatctgtatc acgcgtaggc ggaaatgccc
      301 aaataaaagc aatgaaaaag attgcaggga cactcaaact tgaccaagct cagtaccgcg
      361 aactagaagc tttttcaaaa tttggagcag atcttgatgc tggtaccaag ttggtgctaa
      421 ataaaggaag taaaaatgtt gaaatattaa aacaaaaact tcatcaacca atgcctgtgg
      481 aaaagcaaat tgcaattata ctttgtgggg tacgcgggta tttgtctcat gtaccactaa
      541 ataaggtaca agaatttgaa aataacttgt tagaaattct tgaagctaaa tataaaaaag
      601 atgtattaga catactacaa aagggcgatt tgacagaaga tgttttaaca aaaattaaag
      661 atgtcgcttt tacactttgt aaaaaatatg aataatctaa aaattgtgag taaaaaataa
      721 tggcttcttt aaaagatata cgtgcacgga ttctttctgt tagatcaacg aaaaaaatta
      781 ccagtgcaat gaaaatggtt tcttcatcga aattgcataa aacagagaca ttgtgcctta
      841 attttttacc atacaaaaat gctttgtgtg aaagtttagc taactatttg aactcattag
      901 acgaaaaaat atctgttcaa gtagctgaac aacgcccaat taagcgtatg gcaattatat
      961 gtttttcttc caattcaggt ttatgtggta cgtttaacac aaatattgca aaattagcat
     1021 ctgaaaaaat tgatcaatac gcttatttag gaaaggaaaa tatctatgtt tatactattg
     1081 ggaaaaaaat aactgactac ttgcaaaaac tcaagtaccc tatccatgcc cactacaata
     1141 atttattaga taaccctagt tatgatgaag ttgtaaaatt ggcggatatg ttgactgaac
     1201 tatttatttc aaaacaaata gactgtgtag agttattgta taatcactac aaaaacactg
     1261 cttgccaaac acctacatct gaaatactcc ttccactatc atctatgcaa cttacaaaaa
     1321 ctacgaaatc tcaaccgctg tatattgtgg aaccctctaa agaagaattt attaatgccc
     1381 tagcaccgaa attaattcga atcaaattat atgctgcttt tttagattca tttacagccg
     1441 agcatgctgc tagaactact gcaatgcaaa ttgctaccga aaatgccgaa gaaatgatta
     1501 agacaataac tcaacaatac aataaaaaac gacaagaagt tattaccaat gagttgttag
     1561 atattgtagg aggatctgag gctttacgtc aagattaaca ctaaactgaa aacaatggaa
     1621 ataactagag tttttgatat tttagaaaat tataaaatta taaaaccaaa ccaacctata
     1681 gcactagcac ataaaaaaga aggcaactgg ataaaataca gtattgacga atacatctac
     1741 caaacaaatt taatatcagc aggattgcta tacaatgggg taaaaaaagg agacaccatt
     1801 gctataataa gctctaatag agctgaatac aatattgtag atatggctgc aatgcaaatt
     1861 ggagcaatac ctgtccctat ttatcctacc attagtgaat ctgattatcg atatatttta
     1921 aaccatgccg aaataacgtt tatattttta gaaggagaag aattattacg caaaatagaa
     1981 catattgttc ctgaaatacc tacgctaaaa ggaatttata catttgtaaa cagagggcgt
     2041 tttcattatt ttgagcaatt aataaactta ggaaaggaaa atctcgaaat tgataaaatc
     2101 aatgcaataa aagcaacaat taccccgaat gacgtagcct gtatgatata cacttctggt
     2161 actacaggaa atcctaaagg agtaatgcta agccaccaaa atattacaat gcaactcaaa
     2221 atgttgcaaa atacaccagc gaaatggagc gatacagcat ttagtttttt gcctatttgt
     2281 catgcatacg aaaaaatgat agtttatctc tatcaatact tgggaatgtc tgtatattat
     2341 gccgaaagtt tagctaccat atccgaaaat attaaggaaa tacatcctac
//
LOCUS       DAOSMY010000243         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44539_length_2384_cov_2.482611, whole genome shotgun sequence.
ACCESSION   DAOSMY010000243 DAOSMY010000000
VERSION     DAOSMY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44539_length_2384_cov_2.482611"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLD76_05665"
     CDS             complement(<1..408)
                     /locus_tag="PLD76_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU27524.1"
                     /translation="MNENTLTKMKQMTLHGMHNAFKTAIESGKTDHYTLDQLVSMLID
                     AEWDERHNRRIERSIKNAKFHYQSSIETLNFDTSRNLDRNLVLRLSECEFVEKNENIL
                     ITGSTGVGKSYLGTALGYQACIEGYKVNYFNTSK"
     gene            complement(461..2014)
                     /gene="istA"
                     /locus_tag="PLD76_05670"
     CDS             complement(461..2014)
                     /gene="istA"
                     /locus_tag="PLD76_05670"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOU27525.1"
                     /translation="MANTLTDMSKIRKVLKLYGKGNSKLFISTYLSLSRNTVKKYISL
                     FEVSGKTIEEILAKSDSELELLFSQGKEDAVSPKLQVLYDYFPKMERDLKKVGVTIHH
                     LWEAYIALYSDGFKSSQFRHHYRIWSNRVNPVMHMQHKCGDKMYVDYSGKTLSIIDKT
                     NGESQEVQFFVALLGGSQYTYAEATLSQQKEDFVRSVERAMRYFQGVPAAIVPDNLKS
                     AVIKSSRFEPTINETLADLAEHYDTTILPTRAYKPRDKSLVEGAVKILYRRIYANLKD
                     ASFYSLEEINQAINELLEAHNGRKLTARPYSRRDLFLEDEKPTLRPLPATYFEIKYQS
                     FATVMQNGHVHLSRDKNYYSVPYPYIKKKVKLLYTSSTVEIYYKYNRIALHKRNYKPY
                     VYTTTSEHLASTHRFVAEWSAERFMDWAKSIDTAVGEYILHIIESRNHPEQAYKSCLG
                     ILNFEKKVGKVRLVKACKRGLEFETYSFKSIQKILENGLDYDQNQEENPIPQELPEHS
                     NIRGKQYYN"
     gene            2170..>2384
                     /locus_tag="PLD76_05675"
     CDS             2170..>2384
                     /locus_tag="PLD76_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU27526.1"
                     /translation="MVVILTVFSNQACIEGYRVNYFNTSKLFAKLKIAKADGSYLREL
                     AKIQRQDVIILDDFGLQPLDSQNRITL"
BASE COUNT          628 a          471 c          464 g          821 t
ORIGIN      
        1 cttcgaagta ttgaagtaat ttaccttata accctctata catgcttgat agcccaatgc
       61 tgttcctaga taacttttac caacacccgt gcttcctgtg atgaggatgt tttcgttttt
      121 ttctacaaat tcgcattccg agagtctcaa tacaagattg cgatccagat ttcgggacgt
      181 gtcaaaattt aaggtttcaa tgctggattg gtaatggaat ttggcatttt tgatgcttct
      241 ttcaatgcgc ctattgtgcc tttcgtccca ttctgcatca atgagcattg ataccaattg
      301 atccagcgtg taatggtcgg ttttaccact ttcaatagcg gttttaaagg cgttgtgcat
      361 accgtgaagc gtcatttgct tcattttggt aagggtgttt tcgttcataa ttttaaaatt
      421 taatggttga taattgataa ttttgctaaa cttctaactt ctagttgtaa tactgttttc
      481 cacgaatgtt actgtgctca ggaagttctt gtggaattgg gttttcttct tgattttggt
      541 cgtaatctaa accgttttct agtatttttt gaatggattt aaaactgtag gtctcaaact
      601 ctaaacctcg tttgcaagct ttgacgaggc gcacttttcc tacttttttt tcaaaattta
      661 aaatccctaa acagcttttg taggcttgtt ctggatggtt tctgctctcg atgatatgca
      721 gaatgtattc tccaactgct gtgtcaatgc ttttcgccca atccatgaag cgttctgcac
      781 tccattctgc tacaaaacgg tgcgtgcttg ccaaatgttc cgaagtggtg gtatatacgt
      841 aaggtttgta attgcgcttg tgtagtgcaa ttctgttata tttgtaataa atttctaccg
      901 tagaactggt gtacaatagt tttacttttt tcttgatata aggatatgga acactgtaat
      961 agttcttatc acggcttaaa tgaacatgtc cgttctgcat taccgtggca aaggattggt
     1021 atttgatttc aaaataagta gcaggaagcg gtcgtaaggt gggcttttca tcttccaaaa
     1081 aaagatccct gcgagaataa gggcgagcgg tgagttttcg accattatgg gcttctagca
     1141 attcatttat tgcttggtta atttcttcca agctataaaa tgaagcatct ttcaaattag
     1201 cataaattct tcggtacaaa atcttcacag cgccttctac caatgatttg tctctcggtt
     1261 tgtaggctct ggttggtaaa atggtggtgt cgtaatgttc ggctaaatcg gctaacgttt
     1321 cgttaatggt aggctcgaaa cgactacttt taatgacggc agattttaaa ttatcgggta
     1381 ctattgcagc tggaacgccc tgaaaataac gcattgctcg ttctacagaa cgtacaaaat
     1441 cttccttctg ttggctcagc gtggcttccg catacgtgta ttgacttccg cctaataggg
     1501 ctacaaaaaa ttgtacttct tgggattctc cattcgtttt gtcaatgatg gaaagggttt
     1561 ttcctgaata atctacatac attttatccc cacatttgtg ttgcatatgc atcactggat
     1621 ttacccgatt gctccatatt cggtaatgat ggcggaactg tgagcttttg aaaccatcgg
     1681 aatacagcgc aatgtacgct tcccataaat gatgaatggt aacgcctact ttcttcagat
     1741 ctctttccat ttttggaaaa taatcataca acacctgtag tttgggacta accgcatctt
     1801 ctttgccttg tgaaaaaagt aattccaact ccgaatcgct ctttgctaaa atttcttcta
     1861 tcgttttacc tgaaacttca aataacgaga tatacttctt tactgtgttc ctagataggg
     1921 ataaataagt gcttataaac aacttactgt tccctttgcc gtacaattta aggacttttc
     1981 ttattttact catgtctgtt aaagtgtttg ccataattcg ttattttttt aacgaatgta
     2041 tgggacaaac gacatgaaaa aaaatagtgg tttttagacc tatttccaac ctcaaaatca
     2101 ggtggtcaat ttaatccgaa atgagctgct cactttgctc cgaaaatggt ggtcatttta
     2161 aaccgaaata tggtggtcat tttgaccgtt ttttccaatc aggcgtgcat cgagggatac
     2221 agggtgaact acttcaacac ctcgaagctc ttcgccaaac tcaaaatagc taaagccgac
     2281 ggctcctatc tgcgagaact cgccaagata cagcgccagg acgtgatcat actggacgat
     2341 tttgggctgc agccattgga cagccagaac aggatcaccc tttt
//
LOCUS       DAOSMY010000244         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44564_length_2383_cov_2.414519, whole genome shotgun sequence.
ACCESSION   DAOSMY010000244 DAOSMY010000000
VERSION     DAOSMY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44564_length_2383_cov_2.414519"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1260
                     /locus_tag="PLD76_05680"
     CDS             34..1260
                     /locus_tag="PLD76_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27527.1"
                     /translation="MYGQLQRKLEYDTANVLVPAAYQRMGVGTQVRYQHDAFTIGSSI
                     FLAKDNETSLLNQPDSVGIFPQQNLASQVDISANVATNIRLMGEYAFSLLDRDMRIPS
                     NGSRVYYQSYNMGIAYTFLKNELQFKYERIDPQYSTLGTYYSNSDYENITVNFTTQLL
                     KDKVQLATNVGVQNDDLDGTKGSRTSRFVGAMNVAYTPSQRVSINAGYSNFQSHKNVK
                     SQFNYINQYDIVQNLDTLDFVQLSQNANFMCSYVLQQTDSQNQNVQFTTNWQQAKDTY
                     IGISNPSNFMSMLNSYLMHTIQFNKTNWQVSSSFNYTYSDMQQATNTMFGPTVAVSAR
                     FLNKTLQSGLSYSCNWSYTEAEYDQFVTNIRIHSSYTFLKKHTLQASGTYQLLAKNTT
                     PNIYRTNLTIAYRYTF"
     gene            1263..>2383
                     /locus_tag="PLD76_05685"
     CDS             1263..>2383
                     /locus_tag="PLD76_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27528.1"
                     /translation="MRNRQHTPTLPYNKSKLKERHSFILYLIPFFVFLFCNSLGLYAQ
                     QASKNEANNVLTAIESSQRYIDKLSINDLSVLPVGMQKTIGNIKVIIAVYDITYHSEY
                     ASMSVYAKMLIPQGDGTELFFGMEDVKLSYDGGIIGDAKLKLLGDITIPFDSNTKLVL
                     KGSFQPKTEIKEDLTYAVLTCDGISEIGVSGEVQFATSFITKVNNETATNEAVIGAFK
                     TVITDWNDLLVNINLPSFAIVGLDDYIFSCNNVVLDFSDTRNAQGMVFPETYKELYLP
                     TGNETLWRGVYATAISMKLPEQFTRSDKPKERISIDAQHLIFDHNGVSGLFATNYAIL
                     PYDNGTASGWNFSVERFRLGMLANRVSEASFGGAIGIPLG"
BASE COUNT          789 a          417 c          458 g          719 t
ORIGIN      
        1 tagttgagtc ctgataaatg gaaaattgct gcaatgtatg gtcagttaca gcgtaagtta
       61 gagtacgata cagccaatgt actagtacca gcagcttatc agcgaatggg ggttggcaca
      121 caggtgcgtt atcaacacga tgcatttacg attggttcga gtattttttt agccaaagac
      181 aatgaaacct ctttgctcaa tcaaccagat agcgtaggta tttttccaca acaaaatttg
      241 gcttcacaag tagatatctc tgccaatgtc gctaccaata tacgattgat gggcgagtat
      301 gcgtttagtt tactcgatag agatatgcgt attccttcaa atgggagcag ggtttactat
      361 caatcttata atatgggtat agcctataca ttcttgaaaa atgagttgca atttaaatat
      421 gaacgtattg atcctcaata cagtaccttg ggcacgtatt atagcaatag cgattacgaa
      481 aatataacgg tgaattttac tactcagttg ttaaaagata aagtacaatt agccacaaat
      541 gttggtgtgc aaaacgacga tttggatggc acaaaaggaa gtagaacttc tcgatttgta
      601 ggtgcgatga atgtggcata tactccgagc caacgtgtat caataaatgc tggatattca
      661 aattttcaat cgcataaaaa tgtcaaatct caattcaatt atatcaacca atatgatatt
      721 gtccaaaact tagacacctt agattttgta cagttgtctc aaaatgccaa ttttatgtgt
      781 tcgtatgtgt tgcaacaaac tgacagtcag aaccaaaatg tgcaatttac taccaattgg
      841 cagcaagcaa aagatacgta tattggcata agcaatccat caaactttat gtcgatgctc
      901 aattcttacc tgatgcatac gatacaattt aacaaaacca actggcaggt aagcagttcg
      961 tttaattata cctattcaga tatgcaacag gctacaaaca cgatgtttgg tcctactgta
     1021 gctgtttcgg caagatttct aaacaaaaca cttcagtcgg gcttatcata ttcgtgcaat
     1081 tggtcgtaca cagaagctga gtatgaccag tttgtaacca atattcggat acattcgagt
     1141 tatacatttc tcaagaaaca cactttacaa gcaagtggca catatcaatt attagccaaa
     1201 aatacaacac caaatattta tagaaccaat ttaaccatag cttatagata tacattttaa
     1261 ctatgagaaa tagacaacac acccctacat tgccatacaa taaaagtaag ttgaaagaga
     1321 gacactcttt tatattgtat ttgataccat tttttgtatt tcttttttgt aactcgctag
     1381 gtttatatgc tcaacaagct agcaagaatg aagctaataa cgtgctaacg gcaatagaat
     1441 catcacaacg gtatattgac aaattatcca taaatgattt aagtgtattg cctgttggta
     1501 tgcaaaaaac cattggaaac ataaaagtaa tcatagcggt gtatgatatc acgtaccact
     1561 cagaatatgc ttcgatgagt gtgtatgcaa aaatgcttat tccacaaggc gatggcactg
     1621 agttgttttt tggtatggaa gatgtaaaat tgtcatacga tggaggaatt ataggtgatg
     1681 ccaaattaaa actattgggc gatattacta ttccgttcga tagtaataca aaattggtat
     1741 tgaagggctc attccaaccc aaaacagaaa taaaagaaga ccttacttac gcggtgttaa
     1801 catgcgatgg tatttcagaa attggggtaa gcggagaagt gcagtttgca acatcattta
     1861 taaccaaagt gaataatgaa accgctacaa atgaggcagt aattggtgca tttaaaaccg
     1921 taattactga ttggaatgat ttgttggtga atattaattt gccaagtttt gcaattgttg
     1981 ggcttgacga ttatatattt tcttgcaata atgtagtgtt agattttagc gatacacgca
     2041 atgcacaagg tatggttttc cctgaaacct ataaagaact atatttacct acaggcaatg
     2101 aaacattatg gcgaggcgtg tatgctactg ccataagcat gaaactgcct gaacaattta
     2161 ctcgctcaga taaacccaaa gaacgtattt cgattgatgc tcaacatctt atttttgacc
     2221 ataatggggt atctggtctt tttgccacta actatgccat actgccgtat gataacggaa
     2281 cggcgtcagg ctggaatttt tctgtcgaac ggtttaggtt agggatgtta gccaatagag
     2341 tttccgaagc ctcgtttggc ggagctattg gaattcccct tgg
//
LOCUS       DAOSMY010000245         2373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44822_length_2373_cov_3.599223, whole genome shotgun sequence.
ACCESSION   DAOSMY010000245 DAOSMY010000000
VERSION     DAOSMY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44822_length_2373_cov_3.599223"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLD76_05690"
     CDS             complement(<1..241)
                     /locus_tag="PLD76_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27529.1"
                     /translation="MKNLLLISILFINAIIMQSQVVTETRIETSDDILVIQIDGLPNI
                     CIRNGNIYKVEVEAEKKNQSHIKTSDLEIFSTIFPG"
     gene            complement(266..682)
                     /locus_tag="PLD76_05695"
     CDS             complement(266..682)
                     /locus_tag="PLD76_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOU27530.1"
                     /translation="MNNDILDIISVFKANNLKTSYNKYNDSVRESCYGDKKNQATINF
                     NGDVYKCTARDFKPENREGVLMDDGTIEWNDKYYQRMESKFNNPPCLACNILPICNGS
                     CTQHALDNIGKDYCVHDFDENKKIDIIREKLLYAIN"
     gene            complement(728..1486)
                     /locus_tag="PLD76_05700"
     CDS             complement(728..1486)
                     /locus_tag="PLD76_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOU27531.1"
                     /translation="MKVVKFAILVPIVFVLACSSKEPKLADVVDTGVVGKMIKVEGGK
                     FMMGCSDCAVDEAPVREVTLSTFYIGEFEVLQEEWIAIMEEENPSYHQGDSLPVEQVS
                     WNQVSTFITKLNEKTGKTFRLPTEAEWEFAARGGIKSMGYTYSGSNDINEVAWYLDNN
                     EEQETQKVGQKLPNELGLFDMSGNVAEWVQDRYWVYSEAAETNPAGPSSGTTRVFRGG
                     RCDYEASDCTVTARRSDRPANKYYNLGFRLVMVP"
     gene            complement(1505..>2373)
                     /locus_tag="PLD76_05705"
     CDS             complement(1505..>2373)
                     /locus_tag="PLD76_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU27532.1"
                     /translation="FVDTNGKGGMFTNGWVGFAESHDEERLGYKMITWGAGDLKTNES
                     ARLKRVPLVMAFVSFIPGPKMLWQFQEMGYDYSIEYGGRTSRKPIPWTAFNSNQAKQE
                     AYEASAKIISLRTNYPQVFKEENVTVQATASYWSNGRRIVAQHPDMNVVAIGNFDATA
                     TITATPGFTKTGTWYELLSGTVLNVKNVNRTISLAPGELLVYTDRIPVSVPETLQDET
                     MTYVYPTIAKDYIYVSSLQDILQVNIYSLQGVLVKSEANSEIAISDLYSGYYIVQVVQ
                     ADGVSIHKINKQ"
BASE COUNT          671 a          488 c          354 g          860 t
ORIGIN      
        1 ttccggggaa aatagttgaa aaaatttcta aatcagatgt ttttatatgt gattgattct
       61 ttttttcagc ttccacttct actttgtata tgttgccatt tcttatacaa atatttggta
      121 atccatctat ttgtataacc aaaatatcat cagaagtttc gatacgtgtt tctgttacta
      181 cttgtgattg cataatgatt gcattgataa ataaaatact gattaataat aagtttttca
      241 taaaatgtag gtttttaatt tgtttttagt taatggcgta taataatttt tcgcgtataa
      301 tatctatttt tttattctcg tcaaaatcgt gtacgcaata atctttacct atattatcta
      361 atgcatgctg agtacatgaa ccattacaaa taggtaggat attacaagca aggcagggtg
      421 gattattgaa ttttgactcc atgcgttggt agtatttatc gttccactct atcgtcccat
      481 cgtccattaa aacaccttca cgattctctg gtttaaaatc tcttgccgtg catttatata
      541 catcaccatt gaagttaata gtggcttggt tttttttgtc cccataacac gattctctga
      601 cagagtcatt gtatttattg taacttgttt tgagattgtt tgctttaaaa acacttatta
      661 tatctaaaat gtcgttgttc aaaagccgat gaacatcggc ttttgtttat tgaaattaga
      721 atatctgcta tggcaccatt accagcctga atcctaaatt gtaatattta tttgcaggtc
      781 tatcactgcg tctggcagtt accgtacagt cgcttgcctc gtaatcacat cttcctcctc
      841 taaacacacg agttgttcct gagcttggtc cagcagggtt cgtttctgca gcttcgctat
      901 acacccaata cctatcttgt acccattcgg ctacgtttcc actcatatca aataatccaa
      961 gctcgttggg aagtttttga ccaacttttt gggtttcttg ttcttcgtta ttatccaaat
     1021 accaagccac ttcgtttata tcattgcttc cgctataggt atatcccatt gatttaattc
     1081 ctccacgtgc agcaaactcc cattcagcct cggttggcaa acgaaatgtt ttacctgttt
     1141 tttcgtttaa ctttgtaata aatgtggata cttgattcca cgatacttgt tccacaggca
     1201 ggctatctcc ttgatgatac gatggatttt cttcttccat tatagctatc cattcttctt
     1261 gtaacacctc aaactctcca atataaaagg ttgaaagggt aacttcacgt acaggagctt
     1321 catcaactgc acaatcacta caacccatca taaattttcc gccttcaact ttaatcattt
     1381 ttccgaccac acctgtatct actacatctg ccaattttgg ctctttggac gaacacgcca
     1441 gaacaaatac aataggaaca agaatagcaa atttaactac tttcatacga tttttatttg
     1501 agtattattg tttattaatt ttatgaatag aaacgccatc tgcttgcact acttgaacaa
     1561 tgtaatatcc actatataaa tcacttattg caatttctga gtttgcttct gatttaacta
     1621 aaacaccttg gagcgaataa atatttacct gcaaaatatc ttgtaacgaa gacacatata
     1681 tataatcctt ggctatagtt ggatacacat aagtcatcgt ttcgtcttgt aacgtttcag
     1741 gcacacttac aggtattcta tctgtataca caagtaattc tccaggagct aacgatattg
     1801 ttctattcac attcttaaca ttcaaaaccg ttcctgataa taattcatac catgttccag
     1861 ttttagtaaa acctggcgtt gcagttatag ttgcagtagc atcaaagttt cctatagcta
     1921 ctacattcat atctgggtgt tgtgctacta ttcttcttcc atttgaccaa taggaagcag
     1981 tagcttgtac agtaacattc tcctctttaa aaacttgagg gtaattggtt cgtaaactta
     2041 tgattttagc tgatgcctca taagcctctt gtttggcttg attagagtta aacgctgtcc
     2101 aaggtattgg tttacgtgat gttcttccgc cgtattctat cgaataatca tatcccattt
     2161 cttgaaattg ccataacatt tttggaccag gaatgaatga cacaaatgcc attaccaatg
     2221 gaactctttt taatctggct gactcatttg tttttaaatc accagcaccc caagttatca
     2281 tcttatagcc cagacgttct tcatcatggc tttctgcaaa accaacccat ccgttggtaa
     2341 acattcctcc tttaccattc gtatcaacaa aat
//
LOCUS       DAOSMY010000246         2372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44849_length_2372_cov_4.023738, whole genome shotgun sequence.
ACCESSION   DAOSMY010000246 DAOSMY010000000
VERSION     DAOSMY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44849_length_2372_cov_4.023738"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLD76_05710"
     CDS             complement(<1..328)
                     /locus_tag="PLD76_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOU27533.1"
                     /translation="MNIAALVSGGVDSSVVVHRLKEMGYQPTIFYIQIGMEDETGYMG
                     CTAEEDIEIVSFIAKKYGLSMEVVSLQKEYWDQVVDYTIETVKKGLTPNPDIMCNKLI
                     KFGSFNQ"
     gene            complement(333..1451)
                     /gene="dnaJ"
                     /locus_tag="PLD76_05715"
     CDS             complement(333..1451)
                     /gene="dnaJ"
                     /locus_tag="PLD76_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU27534.1"
                     /translation="MAKRDYYEVLGISKNATEDEIKKAYRKKAIEFHPDKNPGNTEAE
                     EKFKEAAEAYDVLSDPQKKQRYDQFGHAGMGGAASSGGGFGGGMSMDDIFEHFGDIFG
                     GHFGFGSSSRQRTRVNKGTNLRVKVKLTLQEVAKGVEKKIKVKKYVTCSKCGGSGAED
                     ASSIVTCSSCKGTGSVTRVVNSMFGQMQTSSACPTCQGEGKIITKKCSKCNGEGVEIA
                     EEVISINIPAGVQEGMQLSMSGKGNAARRGGVNGDLLILIEEEPHPELIRDDNDLIYN
                     LMLSLPTAILGGTVEIPTIEGKAKIKIESGTQSGKILRLKGKGLPSVNSYGTGDLLVN
                     IGVYIPESLTKEEKKIFEGFEKSQSFSPSKSSKKNFFS"
     gene            complement(1472..2011)
                     /locus_tag="PLD76_05720"
     CDS             complement(1472..2011)
                     /locus_tag="PLD76_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU27535.1"
                     /translation="MKKDKKLEETEESIQETSEQPEIAQNTQEEVSEIDSLKSQLEQV
                     NNSYLLLLADFDNYKKKTLKEKSDLLKYGGENVLSNLLPIIDDFERAMQSLEKEPDSA
                     IKQGIELIHGKFISFLQRNGVKEIDTKEKIFDTDYHEAITLFPAQQPEDKGKIVDCVQ
                     KGYQLHDKVIRYAKVVVAE"
     gene            complement(2128..>2372)
                     /locus_tag="PLD76_05725"
     CDS             complement(2128..>2372)
                     /locus_tag="PLD76_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU27536.1"
                     /translation="LMFLVFFNEYASGLSYYYFISTLFTILQTYIFRLFIDDEKLLKK
                     LNENKKKPSKKSRFAEKLEQLQKQQLELQKQQRNKK"
BASE COUNT          683 a          475 c          304 g          910 t
ORIGIN      
        1 tttgattaaa acttccaaac ttaatcaatt tattgcacat tatatctgga tttggtgtca
       61 agcctttttt tactgtttcg atggtataat ctactacctg atcccaatat tctttttgca
      121 aagaaacaac ctccatcgat aatccatatt ttttggcaat aaatgatact atctcaatat
      181 cttcttctgc cgtacaaccc atatagcctg tttcatcctc cattcctatt tgaatgtaaa
      241 agatagtagg ctgatagccc atttctttta aacgatgtac tactactgag ctatctactc
      301 ctcctgaaac taaggctgct atattcattt acttaactga aaaaattctt tttcgatgat
      361 ttagaaggag aaaatgactg agatttttca aatccttcga agattttctt ttcttccttt
      421 gttaaagatt ctggtatata cacccctata ttcactaata aatctcctgt tccataagaa
      481 tttacgctag gcaaaccttt gcctttcaaa cgtaatattt tacccgattg tgttccactc
      541 tcaattttaa tttttgcttt cccttcaata gtaggtattt ctacagtccc acccaaaata
      601 gcagttggca atgataacat taaattataa attaaatcat tatcatcacg tattaattca
      661 ggatgaggtt cttcttctat taaaatcaat aaatcaccat ttaccccacc tctgcgtgca
      721 gcatttcctt ttcctgacat agatagctgc ataccttctt gcactcctgc tggaatattg
      781 atagaaatta cttcttcagc aatttctact ccttcaccat tacatttgga gcatttcttg
      841 gtaataattt taccttcacc ttggcaagta ggacacgcgg aactcgtttg catttgtcca
      901 aacattgagt ttacaaccct tgttacactt cctgtgcctt tgcatgatga acatgtaaca
      961 atagaagaag catcctctgc tccactccca ccacatttag aacatgtaac gtattttttt
     1021 accttaatct ttttttcaac acctttggct acttcttgaa gtgttaattt tactttaaca
     1081 cgcaagtttg ttcctttatt taccctagta cgctgacgac tactgctacc aaatccaaaa
     1141 tgtccaccaa aaatatctcc aaagtgttca aaaatatcgt ccatagacat acctccgcca
     1201 aatcctccgc cactagaggc tgctcccccc ataccagcat gcccaaactg atcgtaacgt
     1261 tgtttctttt gcggatcact tagcacatca tatgcttctg ctgcctcttt aaatttttct
     1321 tcagcttctg tattaccagg atttttatca ggatgaaact caatagcttt ttttcgataa
     1381 gcttttttaa tttcatcttc agtagcattt ttgcttatac caagaacttc ataataatct
     1441 ctttttgcca tatatacgtt aatctatatt gttattctgc aacgactact ttagcataac
     1501 gaatcacttt atcatgcaat tgatatccct tttgaacaca atccacaatt ttacctttat
     1561 cttctggctg ttgtgctgga aatagagtaa tggcttcgtg ataatctgta tcgaatattt
     1621 tttctttagt gtctatttct tttactccat tgcgttgcaa aaaggaaata aactttccat
     1681 gaatgagctc aataccttgt tttatagcag aatctggttc tttttctaaa ctttgcattg
     1741 ctctttcaaa atcatcaata ataggaagta aattgcttaa tacattttca cctccatatt
     1801 ttaataaatc gcttttttct tttaatgttt ttttcttata attatcaaaa tcagcaagca
     1861 acagtaggta tgagttattt acttgctcta attgactctt taacgaatca atttcactaa
     1921 cttcttcttg agtattttgt gcgatttctg gttgttcaga agtttcttgt atgctttctt
     1981 ctgtttcctc tagttttttg tctttcttca tggtttttta ctaataatta tctgataagc
     2041 tacatgcaat aagcttgcca atcttttaaa acaagtcatt ttggcagtat ttaaaaaaag
     2101 gtgttctaaa aaagaacacc tttatgctta ttttttatta cgttgctgtt tttggagttc
     2161 taactgttgt ttttgtaatt gttctaattt ttcagcaaaa cgagactttt tactaggttt
     2221 ctttttattt tcatttagtt ttttgagaag tttttcatcg tcgataaaaa gacgaaaaat
     2281 atacgtttgt agaattgtaa ataaggttga aataaaatag taataactca atccagatgc
     2341 atattcatta aagaaaacta aaaacataag tg
//
LOCUS       DAOSMY010000247         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_44980_length_2367_cov_3.696799, whole genome shotgun sequence.
ACCESSION   DAOSMY010000247 DAOSMY010000000
VERSION     DAOSMY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44980_length_2367_cov_3.696799"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLD76_05730"
     CDS             complement(<1..596)
                     /locus_tag="PLD76_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOU27537.1"
                     /translation="MNYNNYCVIMAGGVGSRFWPFSKNSMPKQFLDFFGTGRTLLQMT
                     FDRFAKVVPASNILIVSNKDYKDIILEQLPLIKPSQVLLEPNRRNTAPCVAYAVSHIK
                     AINPKANMVVAPADHLILKEIDFLNVIERSLNFVNEHPNLLTLGIKPNRPETGYGYIQ
                     ISDQAMGDFKKVKTFTEKPNLELAKVFVESGEFFWNSGV"
     gene            813..896
                     /locus_tag="PLD76_05735"
     tRNA            813..896
                     /locus_tag="PLD76_05735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Leu,seq:caa)
     gene            complement(940..1728)
                     /locus_tag="PLD76_05740"
     CDS             complement(940..1728)
                     /locus_tag="PLD76_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOU27538.1"
                     /translation="MNYLLPKQLVSVASDAAEMRAKTTVSKTLVLAFLAGAYIAIGGL
                     LAVMIGAGVPGIAEHNPGIPKFLMGAVFPIGLIMCAIAGADLFTGNTAYFIPPVMSGK
                     LQITDLLRNWTLVYVGNFIGALFVAYAMTYLTGLFDNDPWNAGIMHIAEVKTANPFYK
                     TFLKGIGANWLVALAMWLSFASKDVVGKIVAIWFPVMAFVTLGFEHSIANMFFIPTAI
                     FYGADVSWVSFIFDNLIPATLGNIVGGGLLVGMAYWYVYEKEEK"
     gene            complement(1758..>2367)
                     /locus_tag="PLD76_05745"
     CDS             complement(1758..>2367)
                     /locus_tag="PLD76_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU27539.1"
                     /translation="IEPKTGRPYGADDILKMARNQRPFFGKKGGVTVSGGEPLLQARA
                     LIPIFKALKEDGFNTCIDTNGSVLTDAVKELLTYTDLVLLDIKQMDEEAHQAITGVKN
                     HLTLRFATYLAEIGKPTWIRYVLVPGYTDQQEHLHQLGAFVKDMKNVEKIEIQPYHKL
                     GKHKYEALGWQYQLEDVEMNTAEQLTKAKKILSTYGKEVVVN"
BASE COUNT          715 a          492 c          434 g          726 t
ORIGIN      
        1 acacctgagt tccaaaagaa ttcgccactt tcgacaaaaa cttttgctaa ttcaaggttt
       61 ggtttttcgg taaacgtttt cactttttta aaatcgccca tagcctgatc gcttatttgg
      121 atatacccat agcccgtttc aggacgattt ggcttaatgc ccagagtaag caaattagga
      181 tgctcattta caaaatttaa actacgctct attacgttca aaaaatctat ctcttttaga
      241 atcaagtggt cagctggagc cactaccatg ttggcttttg gattaattgc tttgatgtga
      301 ctaactgcat acgctacaca aggggctgta tttctgcggt ttggctctaa caacacttga
      361 gatggtttga tgagaggtaa ttgttctaga atgatatctt tgtaatcttt gttgcttaca
      421 atgagaatat ttgatgctgg tactactttt gcaaagcggt caaaagtcat ctgaagcaag
      481 gtacgacctg taccaaaaaa atccaaaaat tgttttggca ttgaattttt actgaagggc
      541 caaaagcgac ttcctacccc acctgccata ataacacaat agttgttgta attcatacga
      601 ttgatagttt aattggtgtt gtgaaattac atttttgtaa aaaaatagaa aacatcaggg
      661 ttatttattt tagatatctc actttataaa acccatctac tgaatttgaa ggcaaagata
      721 cggaaagcaa ggtaataaaa aaatattttc agaaaaaaga agtttctgta ttgctcaaat
      781 aaaaatttta tgtacttttg cactctccaa ttgcccagat ggcggaattg gtagacgcgc
      841 tggtctcaaa caccagtgga ttcacttcca tgccggttcg atcccggctc tgggtacgaa
      901 aggacctctt aattgattga ttaagaggtc ttttttatac tatttttcct ctttttcgta
      961 cacataccaa taggccatgc ctacaagcaa gccgccacca acgatattgc caagggtagc
     1021 agggataaga ttatcgaaga taaaggaaac ccaagataca tcagctccat aaaagatagc
     1081 tgtagggata aaaaacatat tggcaatgct gtgttcaaaa ccaagcgtta caaaagccat
     1141 tacaggaaac caaatagcca ctatttttcc aactacatct ttggaggcaa aagacaacca
     1201 catggctaat gctaccaacc aattagctcc aatacctttg agaaaggttt tataaaatgg
     1261 gttcgctgtt tttacttcgg caatatgcat aatgcctgca ttccatggat cattatcgaa
     1321 taagccagta aggtatgtca tggcatacgc tacaaaaaga gccccaataa agttgccaac
     1381 atacaccagt gtccaattgc gtagcaaatc agtaatttgc aatttacccg acataacagg
     1441 cggaataaaa taggctgtat ttcctgtaaa caaatcagca cctgcaatag cacacattat
     1501 cagtccgata gggaataccg cacccattaa gaactttgga atacccggat tatgttcggc
     1561 tatacccggt acacctgcac caatcattac agcaagcaaa ccgccaatgg caatatatgc
     1621 acctgctaaa aaagccaata ctaaggtttt cgatactgtt gtttttgctc gcatctcagc
     1681 agcatcgctc gccacagaca ctaattgctt gggaagtaaa taattcatag ttctatttcc
     1741 tattcgtatt tgttgtgtta gtttactact acttcttttc cgtatgtgga taatattttt
     1801 ttagccttgg ttaattgttc ggctgtattc atctctacat cttcgagctg gtattgccac
     1861 ccaagtgctt cgtatttgtg tttaccaagt ttgtgatagg gctggatctc gattttttct
     1921 acgtttttca tgtcttttac aaatgctcca agttgatgca aatgttcttg ttggtcggta
     1981 taaccaggca caagtacata gcgaatccat gtaggcttgc caatttctgc caaataagta
     2041 gcaaaacgca aggtaagatg atttttaacc ccagtaattg cctgatgagc ttcttcatcc
     2101 atctgtttga tatcgagcaa taccaaatcg gtataggtca ataattcttt taccgcatca
     2161 gtcaacacac tcccattggt atcgatgcat gtgttaaaac catcttcttt gagggcttta
     2221 aatatcggta tcaatgcacg agcttgtagc aaaggttcac ctcccgacac cgtaaccccg
     2281 cctttctttc cgaagaaagg acgctgatta cgagccattt tgagtatatc gtctgcacca
     2341 taaggtcgtc ctgttttagg ctcgata
//
LOCUS       DAOSMY010000248         2356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_45242_length_2356_cov_3.295089, whole genome shotgun sequence.
ACCESSION   DAOSMY010000248 DAOSMY010000000
VERSION     DAOSMY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45242_length_2356_cov_3.295089"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /gene="rlmD"
                     /locus_tag="PLD76_05750"
     CDS             complement(<1..1238)
                     /gene="rlmD"
                     /locus_tag="PLD76_05750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOU27540.1"
                     /translation="MRKKKNLPLLEQITITDVAAEGKAIARYNDMVIFIPFAVPGDVV
                     DIQITRKKNSYAEGKIVTTHSYSTNREAPFCEHFGICGGCKWQNLPYASQLTYKQKQV
                     YDNLTRIGKIQSFDMLPILGSEQTQFYRNKLEFTFSNKAWMNYDMLQAGTPRTAALGF
                     HIPGMFDKVLHIEKCWLQDEISNMIRNTLHEFCQQQHISYFDLKEQHGMMRNVIVRTS
                     TTGEIMVIVVFYEKDQSIINEMLSYLLKKVPSISSLLYIHNTKANDTISDQEIHVFHG
                     SDHIFEQMEGLKFKIGPKSFYQTNSRQAYELYKITRDFASLTGNELVYDLYTGTGTIA
                     NFVANRAQKVIGIEYVPEAIEDAKVNASINNITNTSFFAGDMKDILNESFIKTQGKPD
                     VIITDPPRAGMHDDVIKAIL"
     gene            complement(1316..2254)
                     /locus_tag="PLD76_05755"
     CDS             complement(1316..2254)
                     /locus_tag="PLD76_05755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOU27541.1"
                     /translation="MNQTAPFKVFSGTKSRYLAEKICESIGCKLGEMNIQHFADGEFA
                     VSYEESIRGDEVFLVQSTFPNSDNIMELLLMIDAAKRASARKIVAVIPYFGWARQDRK
                     DKPRVSIGAKMIADMLGVAGINRLITLDLHADQIQGFFNVPVDHLYASSIFLPYIQSL
                     KLKDLVIASPDVGGSKRANTYAKHLGVDLVLCHKTRVKANEVEGLKIIGDIQGKNVIL
                     VDDMVDTAGTITKAADIMISEGALSVRAIASHAVLSDPAIERIEKSKLTEIIFTNSIP
                     LRKESPKIRILSIADLLGDTILRVNTNESISSSYLT"
BASE COUNT          713 a          415 c          403 g          825 t
ORIGIN      
        1 aacaaaattg ccttaatcac atcatcatgc atacctgcac gtggaggatc tgttataatc
       61 acatctggtt taccttgtgt tttaatgaat gattcattca agatatcttt catgtcgcca
      121 gcaaaaaaag atgtattggt aatattgttg atagaggcat ttacttttgc atcttctatg
      181 gcttcgggta catattcgat accaattact ttttgagctc tatttgccac aaagttagca
      241 atagtaccag tgccagtata caaatcatac accagttcgt tgcctgtaag tgaagcaaaa
      301 tcacgtgtaa ttttatacaa ttcgtaggct tgacgactat ttgtttgata aaatgattta
      361 ggtccgattt taaattttag cccttccatt tgttcaaata tatggtcaga accgtgaaat
      421 acatgtattt cttgatcgga aatagtatca ttcgcttttg tattatgaat gtataacaat
      481 gaactgatag atggcacttt tttgagtaaa tagcttagca tttcattgat gattgattga
      541 tctttttcat aaaaaactac aataaccata atctcgcccg tagtgctagt gcgtacaata
      601 acgttacgca tcatgccatg ttgttctttt aaatcgaaat agctaatatg ttgttgttga
      661 caaaattcat gtagagtatt tctaatcata ttggatattt catcttgtag ccaacatttt
      721 tcaatatgta atactttgtc aaacatacct ggaatatgaa aacccaatgc tgctgttctg
      781 ggcgtaccag cttgtagcat gtcataattc atccatgctt tatttgagaa cgtaaactca
      841 agtttattgc gataaaattg agtttgttca gaccccaaaa tgggtaacat gtcaaaagat
      901 tgaatttttc caatgcgtgt taaattatca tacacctgtt tttgtttgta tgttagttgg
      961 gaagcatagg gtaaattttg ccacttacat ccaccacata ttccaaaatg ttcgcaaaat
     1021 ggtgcttcac ggttcgttga ataagaatgc gtagttacta tttttccttc agcatacgaa
     1081 ttttttttgc gtgtaatttg tatatctacc acatcgccag gtacagcaaa tggaataaaa
     1141 attaccatgt cattgtagcg tgctatggct tttccctcgg ctgctacatc tgtaatagtt
     1201 atctgctcta gtaaaggaag atttttcttt tttcgcatag tattgggcgt agtgattaaa
     1261 tagaaataac tacccgaggg tagctatctc tatgtttgta aatagatgtc ttctgttatg
     1321 ttaaataact ggaactaatt gattcattgg tgtttacacg aagaattgtg tcgcctaata
     1381 agtctgcaat actcaaaatt cggatttttg gactttcctt tcttagagga atggaatttg
     1441 taaaaataat ttcggttagt ttcgattttt ctatacgttc tatggcagga tcagacaata
     1501 cagcatgcga agcaatagca cgcactgaga gagctccttc ggatatcatt atatctgcag
     1561 ctttggtaat tgtgccagca gtatctacca tatcatctac taatattaca tttttacctt
     1621 gaatgtctcc aataatcttt aacccttcta cttcgttagc ttttacacgt gttttatgac
     1681 aaagaaccaa atcaacacct aaatgcttag catatgtatt ggctcttttc gatccaccaa
     1741 catcaggaga tgcaatcact aaatctttta atttaagcga ttggatataa ggtaaaaaaa
     1801 tcgatgaggc atataagtgg tcgactggta cattgaaaaa tccttgtatt tgatcggcat
     1861 gtaaatcaag tgttattaag cgattaatac cagctacacc tagcatatct gcaatcattt
     1921 tcgccccaat agatacgcga ggtttatctt ttctatcttg acgagcccat ccaaaataag
     1981 ggataacagc tacaatttta cgggcagaag cacgtttagc tgcatctatc attagtaata
     2041 attccatgat attatcggaa ttgggaaatg tagattgtac taaaaaaact tcatctccgc
     2101 gtatggattc ctcgtatgat acggcaaatt caccatcagc aaagtgttga atattcattt
     2161 cgcctaattt acatcctatg ctctcgcaaa ttttttcagc taagtatctc gatttggtgc
     2221 ccgaaaacac tttaaaagga gctgtttgat ttatgggttt tgttttcact tgtatatagt
     2281 atttttttga ttaataagca aataaaggaa aagcagtcat catatcgttt acttttttac
     2341 gcacactagt tatgat
//
LOCUS       DAOSMY010000249         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_45721_length_2337_cov_3.638913, whole genome shotgun sequence.
ACCESSION   DAOSMY010000249 DAOSMY010000000
VERSION     DAOSMY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45721_length_2337_cov_3.638913"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2138
                     /locus_tag="PLD76_05760"
     CDS             <1..2138
                     /locus_tag="PLD76_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU27542.1"
                     /translation="VCYEVHVRDFTVQPGVVKPELAGTYKGFAEKTDYLKELGITHAQ
                     LLPIQNFFTTDEKNRQFSDTDAPVSNYNWGYDPHNYFTPEGWFSSNAADPYARIRELR
                     ELVQDLHNKGIGVIMDVVYNHTYLVETFENVAPGCYYRYNDTNGISAHSGAGPTVESR
                     RKMVQKLTVESLNHFVTEYLVDGFRFDLMGFIDHETMRLIRQEVGKTYNPNNINELVL
                     QGEAWVFSDLDTDAKATGLHAATTKLNHPKEYLNLGFFNDVSRDAFAGREHQKGFVQG
                     SYGEIDRCVTAILGGVKNFPMKGKAFATEVLTDDYNAFAENPAVCLNYLTVHDGFTLW
                     DKINLSVNDPSGLKRAKVMKLATAMIFTSQGKVILHAGDEFLRTKPLAINDIEKTRAH
                     KTPYTNPEEGIEYFHENTYISNDYTNQLRWDRFTNEFSHIAVPMVEYYQGLIAMRRAL
                     PGLRMFDGDNIRKGITFYGMDIKEGAQTSKPKTLGVTDFSDEKLSKLTLKFTNGPANS
                     TYYIAGEVYDKRTDANTTAATMYAVTFNQAGVGEITFSRDQINNFDLGKWGDSKNLNF
                     KLVKNPGTWESIATAYTGSGNNRLRPMDVDDTSTLTVDLSVVDFATGYVPREVDTFIA
                     YEIDNTLENATAPHISNTNYSRIIVIHNADENAITVDIPTITNPQEWSVIADGNKAGI
                     TPIKKDDTAVKIEMKKVTVPALSMAVIVK"
BASE COUNT          789 a          491 c          455 g          602 t
ORIGIN      
        1 tagtatgtta cgaggttcat gtacgtgact ttaccgtaca accaggagtg gtaaaacccg
       61 aacttgcagg aacgtacaaa ggatttgccg agaaaacaga ttatttaaaa gaattaggca
      121 ttacgcatgc tcaactatta cctattcaaa atttctttac tacagacgaa aaaaaccgtc
      181 agttttctga tacagatgct cctgtaagca actacaactg gggatacgac cctcacaact
      241 actttacgcc agaaggatgg ttttcgagca atgctgccga cccctatgca cgtattcgtg
      301 agttacgcga attggtacaa gacttacaca acaaaggtat tggtgtgatt atggacgtag
      361 tttataatca tacctatttg gtagaaacat tcgaaaatgt agctcctggt tgctattacc
      421 gttacaatga taccaatggt atttcggcac attcgggtgc aggacctaca gtagaatcgc
      481 gtcgcaaaat ggtacaaaaa ttaaccgtag aatcactcaa tcattttgtt accgaatact
      541 tggttgatgg tttccgtttc gacctaatgg gctttattga ccacgaaaca atgcgtctta
      601 ttcgccaaga agtgggtaaa acatacaacc cgaacaacat caacgaattg gtattgcaag
      661 gcgaagcgtg ggtattttcc gatttagaca ccgatgccaa agctactgga ctacatgctg
      721 ctaccaccaa actaaaccac ccaaaagaat acctaaatct gggctttttt aacgatgtat
      781 cgcgcgatgc tttcgctgga cgtgaacacc aaaaaggctt tgtacaagga agctatggcg
      841 aaattgaccg ttgcgttaca gccattttgg gaggcgtaaa aaactttccg atgaaaggta
      901 aagcgtttgc caccgaagta cttaccgacg attacaatgc ttttgccgaa aatcctgctg
      961 tatgcctcaa ctacctaacg gttcacgacg gatttacctt gtgggacaaa attaaccttt
     1021 cggtaaacga cccatcggga ttaaaacgtg ccaaagtaat gaaattggct accgccatga
     1081 tattcacttc gcaaggcaaa gtaattttac atgcaggaga tgagttttta cgcactaaac
     1141 cactagcaat taatgatatt gaaaaaacac gtgctcacaa aactccttac accaaccccg
     1201 aagaaggtat tgagtatttc cacgaaaata cgtatatatc taacgactat accaatcaac
     1261 ttcgttggga tcgttttaca aacgaattta gccacattgc tgtgccaatg gtagaatatt
     1321 accaaggctt aattgctatg cgtcgtgcat tgcctggctt gcgtatgttt gatggcgata
     1381 acatcagaaa aggtattaca ttctatggca tggacataaa agaaggtgct caaaccagta
     1441 aaccaaaaac tttgggggtt actgatttta gtgatgaaaa actcagcaaa ctaacgctca
     1501 agtttaccaa cggtcctgcc aatagcacct actacatagc tggcgaagtg tacgacaaac
     1561 gcaccgatgc caatacaact gcagcaacga tgtatgccgt aacatttaac caagcaggtg
     1621 taggcgaaat tacctttagc cgcgaccaaa ttaacaattt tgacttaggc aaatggggcg
     1681 actctaaaaa tctaaacttt aaattggtaa aaaaccctgg cacgtgggaa agcatagcca
     1741 ctgcctatac aggttcgggc aacaatagac taagacctat ggatgtagat gacactagca
     1801 cactaactgt agatttatca gtagtagatt ttgctacagg atatgtgcct cgcgaagtag
     1861 atacatttat tgcctacgaa atagacaata cgcttgaaaa tgctactgct ccacacatca
     1921 gcaacaccaa ctactcgcgc attatcgtaa tacacaatgc agacgaaaat gccattactg
     1981 tggatatccc aacaattacc aacccacaag aatggagcgt aattgccgat ggcaacaaag
     2041 caggtatcac cccaattaaa aaggacgata cagcagtaaa aatagagatg aagaaagtaa
     2101 cagtacctgc actaagcatg gcggtgattg taaaataata ctattttttt ttgaattcaa
     2161 taaaggcagg atacttcgtt atcttgcctt tttgatttga tggggatagc tattgcctgt
     2221 gaaacagaaa aaaccacaat acgaacaaca aaaaaagacc tatacacttt gttacctaaa
     2281 aaaactttag tatttttacg gaaatatttt aaagaataca aacccaaagg atttaat
//
LOCUS       DAOSMY010000250         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_45993_length_2327_cov_3.242958, whole genome shotgun sequence.
ACCESSION   DAOSMY010000250 DAOSMY010000000
VERSION     DAOSMY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45993_length_2327_cov_3.242958"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLD76_05765"
     CDS             <1..524
                     /locus_tag="PLD76_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU27543.1"
                     /translation="PKKSTKSALETTPTDTRLEETLPIAYINIDSLLLNYNLYKKLSE
                     ELLQQEEKSRGKVTQQAVSLQKEMAEFQKKVENQAFLNAERAQAEQSRLLKQQQELQD
                     LDARLTKELMSKQQKMNEQLKASIDSIILEFNKDNKYHLILSNTGNDNILYGNKAYNI
                     TNEILKLMNVEKE"
     gene            605..1573
                     /gene="fmt"
                     /locus_tag="PLD76_05770"
     CDS             605..1573
                     /gene="fmt"
                     /locus_tag="PLD76_05770"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU27544.1"
                     /translation="MTKEELRIVYMGTPEFAVAPLQSLLANQYNVVGVVTMPDKPAGR
                     GHKLQASAVKQVAIEHNLPILQPEKLKDPFFLEALRSLNADLFIVVAFRMLPEVVWNM
                     PRMGTFNLHASLLPQYRGAAPINWALIRGEKETGVTSFFLTHEIDTGDVLLQRKIAID
                     DTDNAGSLHDKLMQLGAETVVETVAYILSGNTSKTPQNELMNGQQIHLAPKIFKETCR
                     INWSLSCEEIKNFVRGLSPYPAAHTEGLDAKGAPFSAKILHVSTQVCTPEARIGTVST
                     DNKTTLKIACADGWVIIDTIQVAGKKAMQTEELLRGFHLLVPQQLT"
     gene            1570..1932
                     /locus_tag="PLD76_05775"
     CDS             1570..1932
                     /locus_tag="PLD76_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOU27545.1"
                     /translation="MKKYVYIALGTISLGLGIVGIFVPLLPTTPFLLLTAALYCHSSD
                     KLYNKLMNHKTLGPYIHDFRTHKSIPLKTKIISISLVWITIGYSIMCVVPLLFVKILL
                     AAIAIGVTIHILSFKTKK"
     gene            complement(1940..>2327)
                     /locus_tag="PLD76_05780"
     CDS             complement(1940..>2327)
                     /locus_tag="PLD76_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU27546.1"
                     /translation="TKAVHYCVENKLYNVVIMGATGKREDHTLGNISLLALYHSLLHQ
                     VVMISDYGIFSAIDKTTVFSSYEHQQVSLFSILPTCPISVAGLRYPIIQRPLEMWWQG
                     TLNESLGTSFTVQLHDKGLCIVFRKW"
BASE COUNT          816 a          438 c          406 g          667 t
ORIGIN      
        1 ttccaaaaaa atcaaccaaa tctgctcttg aaacaacccc aacagatacc aggttagaag
       61 aaacacttcc tattgcatac ataaatatcg actcattact attgaactat aacttgtaca
      121 aaaaactaag tgaagaatta ttgcaacaag aagagaaatc gcgtggtaaa gtaacacaac
      181 aagctgtttc tttacaaaaa gaaatggcag aatttcaaaa gaaagtagaa aatcaagcat
      241 tcttaaacgc tgaaagagca caagctgaac aatcacgttt acttaaacaa caacaagagc
      301 tacaagatct tgatgcacgt ttaaccaaag aattgatgag taaacaacaa aaaatgaatg
      361 agcaattaaa agcgtctatt gattccatta ttttagaatt taataaagac aataagtatc
      421 atctaatttt aagtaatact ggcaatgata atattttata tggaaataaa gcatacaata
      481 ttaccaatga aattttgaaa ttaatgaatg ttgaaaaaga ataaggtata atttacgtcg
      541 catcaaagct actcttatta tcacaataag agtggctttt ttcattatct actatttata
      601 caatatgacg aaagaagaat tacgcatcgt atatatggga acaccagaat ttgctgttgc
      661 tcctctacaa agcttgttgg ctaatcagta taatgtggta ggagtcgtta ctatgcccga
      721 caaacctgct ggtagaggac ataagctaca agcatctgcc gtaaaacaag tagccattga
      781 acataatctt cccattttgc aaccagaaaa actaaaagac ccattttttt tggaagcact
      841 ccgctctttg aatgctgatt tatttatcgt agtggctttt cgtatgttac cagaagttgt
      901 atggaatatg cctcgaatgg gcacttttaa cttacacgcc tccttattac cacagtaccg
      961 tggagcagct cccatcaatt gggctctgat aagaggagag aaagaaactg gcgttactag
     1021 ttttttctta acccacgaaa ttgatacagg agatgtcctg ttgcaacgaa aaatagctat
     1081 cgacgatacc gataatgcgg gttcgctaca cgataaatta atgcaattag gagctgaaac
     1141 ggtagtagaa acggtagcct atattctatc tggtaatact tcgaaaacgc ctcaaaatga
     1201 gttaatgaac ggacaacaga tacaccttgc cccgaaaata tttaaagaaa cttgtcgcat
     1261 aaattggtca ttgtcgtgcg aagaaataaa aaactttgta cgaggtttat ctccgtaccc
     1321 tgcagcgcat acagaaggct tggatgcgaa aggggctcct ttttcggcta aaatattaca
     1381 cgttagcaca caagtttgca cccctgaagc acgtattgga acggtaagta cggataacaa
     1441 aacgacactc aagattgcgt gtgctgatgg ttgggtaatc attgacacca ttcaagtagc
     1501 agggaaaaag gcaatgcaaa cagaagaact actacgtgga ttccacttgc ttgtaccaca
     1561 acaacttaca tgaaaaaata cgtatatatt gcattaggta ccatctcgtt agggctaggc
     1621 atagtgggca tatttgtacc cttattgcct actactcctt ttttgttgct tacagcagct
     1681 ttgtattgcc atagttcgga taagctatat aacaaattaa tgaatcataa aaccttaggt
     1741 ccctatattc atgattttcg cacacacaaa agtattccat taaaaacaaa aatcatatct
     1801 atatcattgg tttggattac cattggctat tcgattatgt gtgttgttcc tctacttttt
     1861 gtaaaaatat tgttagctgc tatagctatt ggcgtaacaa tacatatttt atctttcaaa
     1921 acaaaaaaat aagcattctt taccactttc taaatacaat acataaaccc ttgtcgtgta
     1981 actgaacagt aaaagatgta ccaagacttt cgttgagtgt accctgccac cacatttcga
     2041 gcggtcgttg tataattgga tatcgtaatc ccgccacact gataggacag gtaggaagta
     2101 tggaaaaaag ggagacttgc tgatgctcat aactagaaaa aacagttgtt ttatctatgg
     2161 cggaaaatat tccataatca cttatcatca ctacttggtg taacagcgaa tggtacaacg
     2221 ccaacaacga aatattaccc aaagtatggt cttcacgctt gcctgtagca cccataataa
     2281 ctacattgta tagtttattt tccacacaat aatgtacagc tttggtt
//
LOCUS       DAOSMY010000251         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46136_length_2322_cov_2.397000, whole genome shotgun sequence.
ACCESSION   DAOSMY010000251 DAOSMY010000000
VERSION     DAOSMY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46136_length_2322_cov_2.397000"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLD76_05785"
     CDS             complement(<1..942)
                     /locus_tag="PLD76_05785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27547.1"
                     /translation="MKKILIISYYFPPSGGSGVQRWLKFVKYLPEFGYDPIVLTVDPT
                     VASYPQIDTTLLQDIPSLVRVERTTTREILSLYKRVSPTKEIPYGGFANENTNTFFSI
                     LSRFVRGNLFLPDPRRGWNRFAYKKACELIEQEQIDTVITTSPPHSTQLIGLKLKKRF
                     PSIQWIADLRDPWRDIFYYKDTYPTWLADKLNAYYERAVLQQADQLITVSNGCKNLFL
                     QHAAVAEKFHVIPNGYDEDDFEQLPVDKTANKFVISYIGVLSGLYCIDGLIDAVANLS
                     ASYKQQLIIRFVGKIAPEIRQKLSEKGTDDMLEFVDYV"
     gene            1146..1793
                     /locus_tag="PLD76_05790"
     CDS             1146..1793
                     /locus_tag="PLD76_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27548.1"
                     /translation="MKQKVGSDAKHQLTLLGGINFPSFDIPHSIGPSAGLAYSYYFIP
                     QIGIRGMLHYHYFDGEKNIPAIYSHTMGACVQGIFNFFSYQSGRPEDAGFSFIPSKAY
                     LVAGVGGLYHQPYINGNSQQSDLSFSIPVGIGTRHPIARRWDLGVEFGWNVTSSDMLD
                     QVAIAESTNDSFGNLFLTATYKFPKHNYGPTRGKQFQNRKTKKCDKRKGCAVAFD"
     gene            complement(1806..2270)
                     /locus_tag="PLD76_05795"
     CDS             complement(1806..2270)
                     /locus_tag="PLD76_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU27549.1"
                     /translation="MKKRNYLIGFILVLLVSCICKEPTEPVVVEDDQIVGTWYIHDYT
                     ISNVSIFAIQQCDVGSWIELKADNTMQALIKCNTPPESEGTWSREGDVINIDADKLFN
                     VNKALLSRIDNQATVLLLTDEELQFEFAAYTTLNIKLMVKVFLSRTPLETVE"
BASE COUNT          717 a          472 c          414 g          719 t
ORIGIN      
        1 tacgtaatca acaaattcga gcatatcgtc tgtccccttt tcgctcaatt tttgcctaat
       61 ctcaggagca attttaccga caaaacgtat tataagttgc tgcttataac ttgctgacaa
      121 attagcgaca gcatcaatca acccatcaat acaatacaaa ccacttaaca cgccaatgta
      181 agaaatcaca aacttgttgg ctgttttatc aacgggtagt tgctcaaaat catcttcgtc
      241 gtaaccattg ggtataacat gaaatttttc ggcgactgct gcatgttgca aaaacaaatt
      301 tttacagcca ttgcttaccg taatcaattg gtcggcttgt tgcaaaactg cacgttcgta
      361 atacgcattc aatttgtctg ccaaccatgt aggatacgta tctttgtaat aaaaaatatc
      421 tcgccatggg tcgcgtaaat cggctatcca ttgtatagag ggaaatctct tcttcaactt
      481 cagcccaatt aattgcgtag aatgaggcgg actggtcgta attactgtat caatttgctc
      541 ttgctcaata agttcacagg cttttttgta agcaaatcta ttccaccctc tacgcgggtc
      601 gggcaagaac aaattgcctc gcacaaaacg agaaaggata gagaaaaaag tgttcgtatt
      661 ttcgttggca aaacctccgt atggaatttc tttagtagga gaaacgcgct tgtacaagga
      721 taatatttct ctggtggttg tgcgttctac acgaaccaat gagggtatat cttgcaacaa
      781 agtggtatca atctgaggat aagaagctac tgtagggtca acagtaagca caataggatc
      841 atacccaaat tcaggcaggt acttcacgaa ctttagccat cgctgcacac ccgacccacc
      901 acttggcgga aaataataac taataatcaa gatttttttc atatgcctag caaatatact
      961 gtgcaaatgt acacatttac gagcgatagt aaatctaatt gctaaaaaaa tctgcttgct
     1021 tgttaatgat attaaataaa taacgtattt ttgtgtcgct aatcgtactg ttatctatga
     1081 aaaaaatcat cgtcatttgt ctttcatgtt tttttggcag cctattcact atccacgcac
     1141 aactagtgaa acaaaaagta ggcagtgatg ctaaacatca acttacgttg ttagggggta
     1201 ttaattttcc aagttttgat attccacata gcattggtcc atcggctgga ctcgcttatt
     1261 cgtattattt tattccacaa attggcatta ggggtatgtt gcactatcat tattttgatg
     1321 gagaaaaaaa tattcctgcc atctattcgc atactatggg tgcgtgtgta cagggtattt
     1381 ttaatttctt ttcgtaccaa tcgggcagac cagaagatgc tggattttca tttattccca
     1441 gcaaagcgta ccttgtggct ggcgtgggag gattatatca tcaaccttac ataaacggaa
     1501 actcgcaaca aagcgaccta tcttttagta ttcctgtagg tattggtacc cgacatccaa
     1561 tagctcgcag gtgggatctt ggcgtagaat ttgggtggaa cgtaacttca tcggatatgc
     1621 tcgaccaagt agctattgca gaatcgacaa atgattcgtt tggtaactta tttttaactg
     1681 caacgtacaa attcccaaaa cacaattacg gtcctacacg tggcaaacaa tttcagaacc
     1741 gcaaaacaaa gaaatgcgac aaacgcaaag gatgtgctgt cgcattcgat taaaaaaata
     1801 attttttatt ctaccgtttc taatggagta cgagataaaa atacttttac catcagttta
     1861 atattcagcg tagtataagc agcaaattca aattgtaact cttcgtctgt taaaagtaaa
     1921 actgttgctt gattatcaat ccgactcaat aaagccttat ttacattaaa caatttatcc
     1981 gcatcaatat tgataacatc tccttctctc gaccatgttc cttctgattc aggtggcgta
     2041 ttgcatttaa tgagtgcctg catggtatta tctgctttca actcaatcca cgaacccaca
     2101 tcacattgtt gaatagcaaa tatactcaca ttagaaatag tataatcgtg tatataccat
     2161 gtgcccacaa tctgatcatc ttctacaact acaggctccg ttggttcttt gcaaatgcaa
     2221 gaaacaagca atacaaggat gaacccaata agataatttc tttttttcat gttttgggag
     2281 tgttagctcc atagttttgc tggatatact cccttatgaa tc
//
LOCUS       DAOSMY010000252         2312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46392_length_2312_cov_3.846699, whole genome shotgun sequence.
ACCESSION   DAOSMY010000252 DAOSMY010000000
VERSION     DAOSMY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46392_length_2312_cov_3.846699"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /gene="ovoA"
                     /locus_tag="PLD76_05800"
     CDS             complement(<1..1688)
                     /gene="ovoA"
                     /locus_tag="PLD76_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HOU27550.1"
                     /translation="MKELITKTIDLQSGNPEEKREEIRAYFLKTWALDELLYTQLRDD
                     SIFYHRGDSLRHILLFYLGHTAVFYINKLFLAKIIHQRINPEFESLFAIGVDEMSWDD
                     LNDKNYNWPSVDAVRAYRNQVKELLIHTIDTLPISLPIDWEHPFWIIMMGIEHERIHI
                     ETSSVLIRQLPLEEVVSGRFGNTCTLSGTAPRNEMIPVKGDTVVMGKPSNHNLYGWDN
                     EYGQHKELVADFSASKYLVSNAEYLVFIEENGYNTANYWTEEGWKWRTYTQAEMPLFW
                     RKKGNKFWLRLVAEEIEMPWNWPVEVNYLEAKAFCNWKTQKEGKTYRLPTEAEYVQLT
                     RVCAVPDELEWETAPGNINLEHFTSACPVDMFALGEFYDVIGNVWQWTETPITGYAGF
                     KVHPMYDDFSTPTFDGKHNLIKGGSFISTGNEATHHARYAFRRHFYQHAGVRLVQSET
                     PLVIQHAEYETDREIANECESQWNTEDARNFDVQLAAYIKEQISHLDTKKVLDINTNT
                     GRLPFLLAQSVAKITAIDFSARFIRMPIQLQENGFMRYIVTDENELVHYREIVI"
     gene            complement(1709..1891)
                     /locus_tag="PLD76_05805"
     CDS             complement(1709..1891)
                     /locus_tag="PLD76_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU27551.1"
                     /translation="MKTNKLTRSDERVIGGVCGGIAEYFDLDVSLIRVVWALVALIGG
                     TGLLAYLIAWLIIPQK"
     gene            2022..>2312
                     /locus_tag="PLD76_05810"
     CDS             2022..>2312
                     /locus_tag="PLD76_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOU27552.1"
                     /translation="MIWNEHIECMSREQLRDLQTARLKETVERVYHNTPFYRKKMQEL
                     GILPSDIKDIDDIQKLPFTTKEDLRDNYPFGLMAVPMSEVVRIHASSGTTGKP"
BASE COUNT          690 a          476 c          415 g          731 t
ORIGIN      
        1 tcaattacaa tttcgcggta gtgcaccaac tcgttttcat ctgttacaat gtaccgcata
       61 aagccgtttt cttgcaactg tatgggcata cgtataaacc gtgccgaaaa atcaatggca
      121 gtaatttttg ctacagattg tgccaacaaa aacggaagtc tgcccgtatt ggtattgata
      181 tcaagcactt ttttagtatc taaatggcta atttgttctt tgatataagc ggctaattgt
      241 acatcaaagt ttcttgcatc ttcggtattc cattgcgatt cgcactcatt tgcaatttct
      301 ctatctgttt cgtattcagc atgttgaatt actaatggcg tttcagattg taccaaacgc
      361 acgccagcat gctggtaaaa atggcgtcta aacgcatagc gagcatggtg agtagcttcg
      421 ttaccagtag atataaacga accacctttg attaaattat gtttcccatc gaaagttgga
      481 gtagaaaaat cgtcgtacat agggtgtacc ttaaaccctg catagcccgt aataggcgtt
      541 tcagtccatt gccacacatt gccaattaca tcgtaaaact cacctaaggc aaacatatca
      601 acaggacaag ctgaagtaaa atgctctaaa ttgatattac ctggagctgt ttcccattcc
      661 aattcgtcgg gtacagcaca tacgcgtgtt agttgcacat attcggcttc agtaggcaag
      721 cgataagttt tcccttcttt ctgtgttttc cagttgcaaa aagctttggc ttcgaggtaa
      781 ttaacttcta cgggccagtt ccaaggcatt tcaatttctt cggctaccaa acgtagccaa
      841 aacttgttgc ctttctttct ccaaaacagg ggcatttctg cttgtgtata tgttcgccat
      901 ttccaaccct cttctgtcca gtagttcgct gtattgtacc cattttcttc aataaaaaca
      961 aggtattcgg catttgacac caagtatttg ctagcactaa aatcagctac tagttctttg
     1021 tgttgtccgt attcattgtc ccaaccgtaa aggttatggt tgcttggttt gcccattact
     1081 acagtatcgc cttttacagg tatcatttca tttcttggtg ctgtgccaga taaggtgcag
     1141 gtgttaccaa atcgtccact aaccacctct tccaaaggta attgacgaat aagtaccgat
     1201 gaggtttcga tgtgaatgcg ttcgtgttca atgcccatca taataatcca aaaaggatgt
     1261 tcccaatcaa taggaagcga gatgggtagg gtatcgatgg tatgaattag taattccttt
     1321 acttggttgc ggtatgccct tacagcatct acagagggcc aattatagtt tttatcgttt
     1381 aagtcgtccc aactcatttc atcaacacct atggcaaata atgattcaaa ctctggatta
     1441 attcgttggt gaataatttt tgctaaaaac agcttattga tataaaatac agcagtgtgc
     1501 cccaaataaa aaagcaaaat atgccgcaaa gaatcgcctc tgtgataaaa aatactatca
     1561 tcacgcaatt gtgtatataa cagttcgtcg agagcccagg tttttaaaaa atacgcacgt
     1621 atttcttcgc gtttttcttc aggattaccc gattgcaggt caatggtttt tgtgattaac
     1681 tctttcatgt attactactc ctgattggtt atttttgggg gattattagc caagcgataa
     1741 ggtaagccag aagccctgtg ccacctatta gagctaccaa agcccataca acgcgaatta
     1801 aactaacatc caaatcaaaa tactcggcta ttccaccgca aactccacca ataactcgct
     1861 catcagaacg tgtaagctta tttgttttca tatcgtttcg ttaattattt acttgcaaaa
     1921 ataatcaata tcttttagaa accaatcaag aaataaattg tttcaaatat ttgtgttaag
     1981 ataaattaaa tatgtatatt tgccttttaa tctaatacta tatgatctgg aacgaacaca
     2041 ttgaatgtat gagtcgcgag cagttaagag atttacaaac agctcgccta aaagaaacag
     2101 ttgaaagagt gtaccacaac acaccttttt atcgcaaaaa aatgcaagaa ttgggtatct
     2161 taccctctga tattaaggat attgatgata tacagaaatt accttttacc accaaagaag
     2221 atttacgtga taattatcct tttggattaa tggcagtacc tatgtcagag gtagtacgca
     2281 ttcatgcctc ttcgggtact acaggtaaac ct
//
LOCUS       DAOSMY010000253         2305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46604_length_2305_cov_3.124444, whole genome shotgun sequence.
ACCESSION   DAOSMY010000253 DAOSMY010000000
VERSION     DAOSMY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46604_length_2305_cov_3.124444"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLD76_05815"
     CDS             complement(<1..899)
                     /locus_tag="PLD76_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOU27553.1"
                     /translation="MINITFPDGSVRQYEKGTTGLQIAESISSRLAQDVIAVGVNDQT
                     TEINRPITTDASIKLYKWDDAEGKHAFWHTSAHLMAEALQELYPHVKFGIGPAIENGF
                     YYDIDTADMVIRENDFPTIEAKITEIVARKENVVREEISKADAIKLFTEKDEPYKIEL
                     ISELEDGTISLYRQGNFVDLCKGPHLANTADIKAFKLLSVAGAYWRGDEKRKQLTRIY
                     AISFPKKKMLDEYLLLLEEAKKRDHRKIGKELELFTFSQSVGQGLPLWLPKGTMLRDQ
                     LEDFLKKVQKKYGYLPVRTPHIG"
     gene            complement(1084..>2305)
                     /locus_tag="PLD76_05820"
     CDS             complement(1084..>2305)
                     /locus_tag="PLD76_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27554.1"
                     /translation="VNKHLESTVGKEFDDYVRYVLSGQNTKFSVLNSQGGNPFKHLIK
                     NSESENTKAFALRQVYKFADTDKTPQKDEMSFTVPYLAAKIVLLSNTSNKQLVVVNYK
                     PSHEPNEHYKVYYGRYDSNLKKVEYTDISDSTSYNILLNTRTANAIDDARNTSFLLFV
                     NTLAPSKTDVFTDFDASFELTASPVIAFTDVDYISVGTDFIHTYSDSDVLMPFYISGA
                     KRSFIAIDGEQILDYFRVDYTNSQISIVNDSTYKVSVQYQHSLSISQNRPNLPASLQT
                     KQLTQTIVYNFVLGKLTIEQHANITNKWGAYYDDFMDTHYPESLGSTRTEHTKISLIG
                     VNTFGMVDSDRLFFGTENTADTRKAILSMSDTSNATYYYSNGEVSGTSSRSYVSTDFS
                     SPSVKIYLWVHYDQ"
BASE COUNT          659 a          380 c          456 g          810 t
ORIGIN      
        1 tgtccgatat gaggtgtacg tacaggcaaa taaccgtatt ttttttgtac ttttttcaag
       61 aagtcttcta attggtcccg tagcatcgtt ccttttggta accaaagagg taagccttga
      121 ccaacagatt gagagaaagt gaaaagttct aattctttac caatttttct gtggtcgcgt
      181 ttttttgctt cttctagtaa taacaaatat tcgtcaagca tttttttctt tggaaacgaa
      241 atcgcataaa tacgagttaa ttgtttgcgt ttttcgtcac cacgccagta tgctccagct
      301 acggaaagca atttaaatgc tttgatatca gcagtattag ccaagtgagg tcctttgcat
      361 aaatcaacaa aattcccttg acggtacagg ctaatggtgc catcttcaag ctcgctgatt
      421 aattcaatct tgtagggttc atctttttca gtaaataatt taatagcatc tgctttagat
      481 atttcttcac gcactacatt ttctttgcgt gcaacaatct cagttatttt tgcttcaata
      541 gttggaaaat cattttcgcg aattaccata tcagctgtat ctatatcgta ataaaaacca
      601 ttttcgatag ctggtccgat gccaaatttt acgtgaggat acaattcttg tagggcttca
      661 gccattaagt gagcagaggt gtgccaaaaa gcatgtttgc cttcagcatc atcccatttg
      721 tataatttaa tagaagcgtc tgtcgttatt gggcgattaa tttcagtagt ttggtcgttt
      781 actccaacag caatcacgtc ttgtgctagg cgtgagctga tgctctcggc tatttgtaag
      841 ccagtagttc ctttttcgta ttgacgaaca gaaccgtcgg gaaatgtaat gttaatcata
      901 tagatagttt atcgaatgtg attgattaaa ttttgtaatg actgcaaagg taaataatat
      961 tttttgaaaa ctatttatcg tttgctattt tatggttttg taacgtttaa aatctgattg
     1021 ttatgtataa aaaacccgtt tcaagcagta ctagttgaaa cgggaaataa tgaataagca
     1081 cttttattga tcataatgca cccacaggta aatttttaca gaaggagaac tgaaatctgt
     1141 gcttacatag cttcttgatg atgttccact tacttcgcca ttactgtaat agtaggtagc
     1201 gtttgatgtg tcggacattg agaggatagc tttacgggtg tcagctgtat tttctgtacc
     1261 aaagaataag cggtcagaat ctaccattcc aaatgtattg actccaatta gggatatttt
     1321 agtgtgttcg gtacgtgttg aaccaagcga ttcggggtag tgtgtatcca taaaatcgtc
     1381 gtaatatgct ccccatttat tggtgatgtt ggcatgctgt tcaatggtta attttcctag
     1441 tacaaaatta tatactattg tttgtgttag ctgcttagtt tggagtgagg ctggtagatt
     1501 tggtctattt tgactaatgc ttaatgagtg ttggtattgt actgaaacct tgtatgtgga
     1561 gtcgtttacg atgcttattt gagaattcgt atagtccacc ctaaaatagt ccaagatttg
     1621 ttctccatct attgcaatga aactgcgttt ggctccagag atgtaaaatg gcatgagcac
     1681 atcgctgtcg ctatacgtat gaataaaatc agtacctaca gaaatatagt caacatctgt
     1741 aaaggcaata acgggagatg cggttaattc aaacgaagca tcaaaatcag tgaaaacatc
     1801 tgttttagat ggagctaatg tgttgacaaa cagtaaaaag cttgtgtttc gagcatcatc
     1861 aatagcattt gccgttcgtg tgttgagtaa aatattgtag gaggtagaat cagaaatgtc
     1921 agtatattct actttcttta aattgctatc atagcgccca taatacactt tgtaatgttc
     1981 gttgggttcg tgcgagggtt tgtaatttac tacaactaat tgtttgttgc tagtgtttga
     2041 taataatacg attttagcag ccaaataggg taccgtaaaa gacatttcat ctttttgagg
     2101 cgttttatca gtgtcagcaa atttatatac ttgacgcagt gcaaatgctt tggtgttttc
     2161 gctttctgaa ttcttaatga ggtgtttaaa aggattacca ccttggctat ttagtactga
     2221 gaatttggtg ttttgtccag ataaaacata acgaacataa tcatcaaatt cttttccaac
     2281 agtactttct agatgtttgt ttaca
//
LOCUS       DAOSMY010000254         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46628_length_2304_cov_3.711872, whole genome shotgun sequence.
ACCESSION   DAOSMY010000254 DAOSMY010000000
VERSION     DAOSMY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46628_length_2304_cov_3.711872"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..738)
                     /locus_tag="PLD76_05825"
     CDS             complement(244..738)
                     /locus_tag="PLD76_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU27555.1"
                     /translation="MNNITTTIIEKMIQYFGTDVRRINHALKVYGFARCIARKEKLTE
                     NELFIVDIVAILHDIGIIEAEKKYNSSNGKYQEIEGPAIAKNMLSDQHIDTTTIDRIC
                     FIIGNHHSYQKIDNMDFQVIVEADFLVNIYEDEMEKHSIENIRNKYFKTQTGIAIIQS
                     MYAD"
     gene            1206..1817
                     /locus_tag="PLD76_05830"
     CDS             1206..1817
                     /locus_tag="PLD76_05830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU27556.1"
                     /translation="MEDTFAHKALTWDNPRKISMTNKFVKEIEKLCSFSSSLKILEIG
                     TGTGLVGLQLVSKASEMLLIDTSPSMLQILEKKLQDNPYPHVKCLHATIETINATDFD
                     LVISFMTWHHIADLSSAFTSVYTLLKKGGKLLIGDLLTEDGSFHGDEFVPHLGFDMQM
                     LRTIAENHGFKVLQVYPYAEVEKQVQNGDINRYGQFVLFAEKI"
     gene            complement(2009..2215)
                     /locus_tag="PLD76_05835"
     CDS             complement(2009..2215)
                     /locus_tag="PLD76_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27557.1"
                     /translation="MIQWQKNWINQWEYSLKSPANLLETYELESFRSYVLDVHDDIGG
                     KSCTPMTIKKLEKYWNKLNIIILH"
BASE COUNT          690 a          348 c          386 g          880 t
ORIGIN      
        1 cgatgttact cttttacgaa cagaacaata tttcgcaatt taaagagata tttatcaatc
       61 agtttgaatt tgcggttaag acttattttt aggatgtgac accattttct ccaagcagag
      121 aatatggttg aaattggctc gggtcaatac agataatgat tcaggtatat tacaatttag
      181 taaataaaaa gtgctattat tctttttctt ctaacgttcg tatttgcgtg aatcatattt
      241 gtttcaatca gcgtacatac tttgtattat tgctatacct gtttgagttt taaagtattt
      301 attccgaata ttctctattg aatgtttttc catttcatct tcatatatat ttactaaaaa
      361 atctgcttca acaataacct gaaaatccat attgtctatt ttctgatagg aatgatgatt
      421 gccaataatg aaacaaattc tatcaatagt tgtagtatct atatgttggt ctgacaacat
      481 atttttcgcg atggcaggtc cttctatttc ttggtatttg ccgttactgg aattatattt
      541 tttttcagct tcaatgatac ctatatcgtg tagtatagcc actatatcca caatgaatag
      601 ttcattttct gttagttttt cttttcgtgc aatacacctg gcaaagccat atactttaag
      661 ggcatgattt attcttcgaa cgtctgttcc aaaatattga atcatttttt cgataatagt
      721 agtagttata ttattcatat ttggtatgtt aacgattgtt gttacagtat gatgtgtgtg
      781 tctactaaaa atggtttttc tgtatctgtt tgcttatgat ggtgataagc ctttgtttcg
      841 cttaattatt gctcaaaagt aagatttttt taaggatagt acaaaataga gggaaagaat
      901 tttttagtta gagtgttgca cttatagata tagcattgct tagtctgcat tctgtcgttg
      961 ttttacatgg tatttgtaaa agactaggat aagttaaaaa tataaaaaag ataatcgaca
     1021 gtgaacatag ccgttgttat tgagcaaaaa atccaattaa attcattttc ttttgattat
     1081 ttcgtatttt atagcgaaat ttgtgaagga tgataatgca ggtgtgtctt atttcaaaca
     1141 tggtctttta aagctctatc ctccattatt ttatgcttac acaggacaat actataccta
     1201 attttatgga agatacattt gcccataagg ctttaacttg ggataatccc cgaaaaatca
     1261 gtatgaccaa caaatttgtg aaggaaatag aaaaattgtg ctctttttct tcttctctca
     1321 aaatacttga aattggaaca ggtacgggtt tggttggttt gcagttagta tccaaagctt
     1381 cagaaatgtt gcttatagat acatcgccat caatgctaca aattctagaa aaaaaattac
     1441 aagacaatcc atatcctcac gttaagtgtc tacatgctac tatcgaaacg attaatgcta
     1501 cagattttga tttggttatt tcttttatga cttggcatca tatagcagat ttgtctagtg
     1561 catttacatc tgtgtataca cttttaaaaa aaggagggaa actccttata ggcgatttgc
     1621 tcacagaaga tgggagtttt catggtgatg aatttgttcc tcatttaggg tttgatatgc
     1681 aaatgttgcg tactattgcc gaaaatcatg gatttaaggt gttacaggta tatccgtatg
     1741 ccgaggtcga aaaacaagta caaaacgggg atatcaatcg ttacggtcaa tttgtgttat
     1801 ttgctgaaaa gatataggca tcaatagtcg tttagatacc aacttgttgt ccgttgtagc
     1861 tggaaacagg gcatcaggaa gtagatctta tgtgattatg tttcatgtag ttgatatcta
     1921 catgcctgct cttttaattt cgtatatttg tcatataatg aagaattgtt tagtttttca
     1981 tctccccaaa acagcccatt tcttattttt aatgtaggat aattatgttt aatttgttcc
     2041 agtatttttc taatttttta attgtcattg gtgtacaact cttgccacca atatcatcat
     2101 gtacatctaa tacataagaa cgaaatgatt ccaattcgta cgtttcgagt aagttagctg
     2161 gtgatttcaa actatattcc cactgattaa tccaattttt ttgccattga atcacatctg
     2221 tatcggcatt gttttttcta accaaattaa tttctcgcta tacgcttcta gtgctagtct
     2281 tttagtgttt tcaaaggttt caat
//
LOCUS       DAOSMY010000255         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46724_length_2301_cov_3.257346, whole genome shotgun sequence.
ACCESSION   DAOSMY010000255 DAOSMY010000000
VERSION     DAOSMY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46724_length_2301_cov_3.257346"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..817
                     /locus_tag="PLD76_05840"
     CDS             47..817
                     /locus_tag="PLD76_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOU27558.1"
                     /translation="MKKYILDCVVVANIPLNKRYFLLRVSHPSPFPRMLPGQFVELRV
                     DNSSDVLLRRPISIHDMNYETNEMDLLVQIVGAGTLKMSELKKGDLLNMIAPLGNSFS
                     MPTIDMKHVLLVGGGVGVAPLLLLGRVLQQYSCKVSFLLGARTASDLVQLDSFSQYGE
                     VYLTTEDATVGEKGFVTNHSLLKNKVFDKIYTCGPTPMMKAISAYAKTNNIECEVSLE
                     NTMACGFGVCLCCVTDTTEGHKCVCTEGPVFNLTTLKW"
     gene            811..1719
                     /locus_tag="PLD76_05845"
     CDS             811..1719
                     /locus_tag="PLD76_05845"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOU27559.1"
                     /translation="MVDLGINIGNLQLKNPVMTASGTFGYGTEFSDFFDISALGGIII
                     KGTTLHHREGNPYPRMAETPAGMLNAVGLQNKGVEYFITSIYPSLKTIDTHVLVNVSG
                     STIEEYVQAAEKINDLDKIPAIELNISCPNVKKGGMGFGTSCASAAEVVKAVRKVYTK
                     SIMVKLSPNVTNIAEIAQAVEAEGADSVSLINTLLGMAVDAEKQKPVLSTITGGLSGP
                     CVKPIALRMVWQVAKAVNIPIVGLGGIMNATDAIEFMLVGASAVQIGTANFIDPTVSL
                     KIVNGIEAYLQKHNYSSVKDIIGLIK"
     gene            1752..>2301
                     /locus_tag="PLD76_05850"
     CDS             1752..>2301
                     /locus_tag="PLD76_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27560.1"
                     /translation="MRILILSYFLFVSHLVIYASDMPVAYARTLDDVLLYTNELQLTQ
                     DSLSNQSVLDTSVAIQYPDFELIINKDSLHLILNRILPAYQYVDSIRSDSVHIFEKML
                     IATIQHPYFVDWVFTRNGYKLQNEIEKQELINKIKSGNQVTIIKQADEVYVYELQNQQ
                     KLRKNTQLSFNDYVFINQDIKPR"
BASE COUNT          735 a          347 c          446 g          773 t
ORIGIN      
        1 cagttttggg tgttttgatt actaaattat tctacctaat gattttatga aaaaatacat
       61 tcttgattgt gttgttgtag caaatatccc gttaaataaa cgctattttc tgttacgtgt
      121 atcacatcct agtccttttc ctcgtatgtt acctggtcag tttgttgaat tgcgtgtaga
      181 caattcttct gatgtgttat taagaagacc tatttctatt catgatatga attacgaaac
      241 aaatgaaatg gatttgttag ttcaaattgt tggagcaggt accctcaaaa tgtcggagtt
      301 aaaaaaagga gatttactta atatgatagc tcccttgggt aattcattct cgatgccaac
      361 aattgatatg aaacatgtgc ttttggtagg aggaggtgta ggagttgccc cattattatt
      421 attgggaagg gtattacaac agtattcttg taaggtttca tttcttcttg gtgcacgtac
      481 tgcgagtgat ttagttcaac ttgattcttt tagtcagtac ggcgaggttt atcttacaac
      541 agaagatgcg actgtaggtg aaaaaggttt tgtaactaat cattcgctat tgaaaaataa
      601 ggtttttgac aaaatttata cttgtggtcc aactcctatg atgaaagcca tttcagcata
      661 cgcaaaaacc aataatattg agtgcgaagt atcgttagaa aatactatgg cttgcggttt
      721 tggcgtatgt ttatgctgtg taacagatac aacagaaggt cataaatgtg tgtgtacaga
      781 aggtccagtg tttaatttaa caacattaaa atggtagatt taggtataaa tattggtaat
      841 ttgcagctta aaaatccagt aatgactgca tccggaactt ttggatatgg tacagaattt
      901 tctgatttct ttgatatttc agcgttaggt ggaattatta ttaaaggaac aacgttgcat
      961 catcgcgaag gcaatccgta tccacgaatg gcagaaactc cagcaggaat gctaaacgct
     1021 gtcggcttgc aaaataaagg agtagagtat tttattacat ccatttatcc atcccttaaa
     1081 acgattgata ctcatgtgtt agttaatgtt tctggtagta caatagaaga atatgtgcaa
     1141 gctgcagaaa aaataaatga tttggataag atacctgcta ttgaattaaa tatttcgtgt
     1201 ccaaatgtaa aaaaaggagg tatgggtttt ggtacatcat gtgcatcagc tgcagaggta
     1261 gtaaaagctg tacgcaaggt atataccaaa agcattatgg ttaaactttc tcctaatgtt
     1321 accaatatag ccgaaattgc acaagcagta gaagctgaag gggcagatag tgtatcgttg
     1381 attaatacat tgctgggtat ggctgttgat gctgaaaagc agaaacccgt tttatcaacc
     1441 attacgggtg gattgtctgg tccatgtgtt aaacctatag ctttacgtat ggtatggcaa
     1501 gtagctaaag ctgttaacat accgattgtg ggcttagggg gtattatgaa tgcaactgat
     1561 gctattgaat ttatgttagt aggtgcttct gctgtgcaga taggaacagc aaattttatt
     1621 gacccaactg tgagcctcaa aatagtaaat ggaatcgaag catatttaca gaaacataat
     1681 tacagctctg taaaagatat aattgggctt ataaaataag tatttaattt tcttttattt
     1741 tcggtctttt tatgcgtatt cttattttat catatttcct ctttgtgtcg catttggtta
     1801 tttacgcttc ggatatgccc gtggcgtatg ctcgtacact ggatgatgtt ttactatata
     1861 ccaatgagct acaattaacc caagattcat tatcaaatca atctgttttg gatacgtctg
     1921 ttgcaattca atatcctgat tttgaattga ttattaataa agatagttta catcttatat
     1981 tgaatagaat attaccagcc tatcaatatg ttgattctat tcgctctgat tcagttcata
     2041 ttttcgaaaa aatgctcata gctaccattc agcaccccta ttttgtagat tgggtattta
     2101 ctcgaaatgg atataaactt caaaacgaga tagaaaaaca agaacttatt aacaaaatta
     2161 aatcaggaaa tcaagtaaca atcattaaac aagcagatga agtttatgta tatgagctgc
     2221 aaaaccaaca aaagttgaga aaaaatacac agctgtcgtt taatgattat gtatttatta
     2281 atcaggacat taaaccccgt c
//
LOCUS       DAOSMY010000256         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46725_length_2301_cov_3.014693, whole genome shotgun sequence.
ACCESSION   DAOSMY010000256 DAOSMY010000000
VERSION     DAOSMY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46725_length_2301_cov_3.014693"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLD76_05855"
     CDS             <1..350
                     /locus_tag="PLD76_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HOU27561.1"
                     /translation="HEKEYIVRVNKPITNDFLQKMSTGVPILDTITKPCTVKKMNEHT
                     FSIILTQGLNRQIRRMCEYLDYGVSTLKRIRIMNISLGSLKKGQFRYFTKRELNELLA
                     LVETSSKTHDTFL"
     gene            438..1664
                     /locus_tag="PLD76_05860"
     CDS             438..1664
                     /locus_tag="PLD76_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOU27562.1"
                     /translation="MKVITLFALGTIMLLNACTSTEPTENALKTITLEEGMEARVDEE
                     NSFAFDFLQETLSTTEEDNVFISPLSVSIAMGMTWNGANGATKEEIKQALKHQGLTDE
                     QINRYYQILLNNLPNLDKKTKLILANSIWYKKDFPILKDFLDKNNVYFNAKVQGLDFS
                     KSESLDIINNWCAEKTNNLIKKPLDQISDNSIAFLINAIYFKGLWKNKFDSKHTQEAT
                     FTAEDNTSTTVDMMNQTESFDYTEDDDAQYIDLSYGNGAFSMLVILPKQGKKINDIAP
                     NLTKTKWKSIISSLNTESIALQLPRFKVQNKMQLNPILVKLGIEKAFLPEQADFSGIS
                     SDAQLYISRVIHQTYCEVNEEGTEAAAVTIVDVAVTSIPILKEFRANKPFLFLIREKS
                     TGIIVFAGKIGKIANE"
     gene            1692..>2301
                     /locus_tag="PLD76_05865"
     CDS             1692..>2301
                     /locus_tag="PLD76_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27563.1"
                     /translation="MKVIYVVIALLTSLLYACAPSTENTEKYSVNEPIFMDATEFRSS
                     IAITQDVHPITSYGKISFYKGYLFVSEPQKGIHIINNTDAKNPQVVGYIELLGNADIA
                     IKDDILYADSYIDLVWFDISTPATPLFVGRLENVFEQALPPLEDPSFGIDGSMVWDRP
                     SDQVIVGWTIATRTIKTSNSIFSGFPWWSSSDPTPIMAEKGST"
BASE COUNT          830 a          390 c          381 g          700 t
ORIGIN      
        1 accacgaaaa agaatatatt gtgagagtaa ataaacccat cacaaacgat tttttgcaaa
       61 aaatgagtac aggagtgccc atacttgata caattactaa accatgtact gttaagaaaa
      121 tgaatgaaca cacattttca attattctaa cacaaggact taacagacaa atacgtcgta
      181 tgtgcgaata tcttgattat ggggtaagta cattgaaacg aattcgtatt atgaatattt
      241 cgctaggtag tttaaaaaaa ggacaatttc ggtactttac aaaacgagag ttgaacgaac
      301 tattggcttt ggtagagact tcgtcaaaaa cacacgatac atttttgtaa aattgtttaa
      361 aacttaacac aataattagg atttttaatt cgtttatgtt atatatttgt atataatttg
      421 ctaaaaatct acttattatg aaagttataa ctttatttgc attgggtaca atcatgctac
      481 taaatgcgtg tacatcgaca gaaccaactg aaaatgcctt aaaaactatc actttagagg
      541 aaggcatgga ggcacgtgtt gatgaagaaa atagttttgc cttcgatttc ctgcaagaaa
      601 cacttagcac aactgaagaa gacaacgtat ttatatctcc attaagcgta agcatagcaa
      661 tgggaatgac gtggaatgga gcgaatggtg caacaaaaga agaaattaaa caagccttaa
      721 aacaccaagg attaactgat gaacaaatta atcggtatta tcaaatcctg ctaaataact
      781 taccaaattt agataaaaaa acaaaactta ttttggcaaa ttctatttgg tataaaaagg
      841 attttcccat attaaaagat tttcttgata aaaacaatgt ttattttaat gccaaagtgc
      901 aaggtttaga tttctctaag tccgaatcgc ttgatattat caacaattgg tgtgcagaaa
      961 aaacaaataa tttaataaaa aaacctcttg atcaaatctc tgataattct attgctttcc
     1021 taattaacgc aatttatttt aaaggacttt ggaaaaacaa atttgatagc aaacacacac
     1081 aagaagctac atttacggca gaggataaca cctctactac tgttgatatg atgaatcaaa
     1141 cagaaagttt tgattatacc gaagatgatg atgctcaata cattgattta tcatacggaa
     1201 atggggcttt ttcgatgttg gtgatacttc caaaacaagg aaaaaaaatt aacgatatag
     1261 ctcctaactt aacaaaaaca aaatggaaat caattataag ttccttaaac acagaatcaa
     1321 ttgcactaca attgccccgc tttaaggtac aaaacaaaat gcaactcaat cctattttag
     1381 tgaaattagg aatagaaaag gcttttttac cagagcaagc agacttttct ggcatatcgt
     1441 ctgatgctca actctatatt tcgcgtgtta ttcatcaaac ctactgcgaa gtaaacgaag
     1501 aaggaactga agctgcagct gttaccatcg tagatgttgc ggttacatct attccaattt
     1561 taaaagaatt ccgtgctaat aaaccatttc ttttcttgat tcgcgaaaaa agcacaggaa
     1621 tcattgtatt tgctggaaaa atcggcaaaa ttgctaacga ataattgcat accatttaat
     1681 attcaacaca tatgaaagta atttatgtag ttatagcatt gttaacatcc ctgttatatg
     1741 cgtgtgctcc gagtactgaa aacaccgaaa aatattcagt caatgaacca atttttatgg
     1801 atgctactga atttcgcagt tctattgcta ttacacaaga tgtacaccct attacatcgt
     1861 atggcaaaat tagtttctac aaaggctatt tattcgtgtc agaacctcaa aagggcattc
     1921 atattatcaa taatacggat gccaaaaacc cacaagtagt tggttatatc gaacttcttg
     1981 gcaatgctga catcgccatt aaagatgata tcttgtatgc ggattcgtat attgatttag
     2041 tatggtttga tatttctact ccagctacgc cattgtttgt tggacgacta gaaaatgtat
     2101 ttgaacaagc tttacctccg ctagaagatc ctagttttgg tatagatgga tcaatggtct
     2161 gggacagacc aagcgaccaa gtaattgttg gttggacaat agccactcgt acaattaaaa
     2221 cttctaactc tatcttttct ggtttccctt ggtggtcatc gtctgaccca acacctataa
     2281 tggctgaaaa aggctcaact g
//
LOCUS       DAOSMY010000257         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46912_length_2294_cov_3.051809, whole genome shotgun sequence.
ACCESSION   DAOSMY010000257 DAOSMY010000000
VERSION     DAOSMY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46912_length_2294_cov_3.051809"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..2068
                     /locus_tag="PLD76_05870"
     CDS             122..2068
                     /locus_tag="PLD76_05870"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOU27564.1"
                     /translation="MYNYVKVAAAVPNLEVANCYFNQESICAKIMEARHLGIEVLVFP
                     ELCITGYTCGDLFFQQTLQKATLDALQRILSATESIDMLVAVGAPLCIDNQLFNCAIV
                     LSKGKIVGIVPKTYIPNYNEFYEQRWFSSAFDLKINTINICSQVIPIGTDLLFSLHNG
                     TKIGVEICEDLWVPIPPSAGLCVSGADIILNLSASNETVAKRQYRRNLVVQQSARCMC
                     AYVYASAGGTESTTDLVFSGHSMIAENGVLLKENAQLIDSDYLLSSHVDIDKLRVDRC
                     KYTTYKETHRAYFKEIRCIPSDVQSKHNVSLDYPISRKPFVPSNLHLRDNRCQEIVAM
                     QVAGLVKRLVHTNAPTVVIGISGGLDSTLALLVCVQAFDELKKDRKGILAVTMPGFGT
                     TNRTYDNALTLIQELGVTRRVIDIKKSCYQHFEDIDHDETIHDITYENTQARERTKLL
                     MNLANKHRGFVIGTGDLSELALGWCTYNGDHMSMYAVNVSIPKTLIRSLVLNIAHTQE
                     EKIKQVLLDILDTPVSPELLPIGKNGEMIQKTEDSVGPYELHDFFLFYVMRYGFSPSK
                     ILFLAKHIFSKKETEEDVLFDDETIQKWLIVFYKRFFSQQFKRNCMPDGPKVGSICLS
                     PRGDWRMPSDAVAHEWLKEISGNL"
     gene            complement(2086..>2294)
                     /locus_tag="PLD76_05875"
     CDS             complement(2086..>2294)
                     /locus_tag="PLD76_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU27565.1"
                     /translation="MATAGSGDVLTGIIAGLRAQKYDAIEASLLGVYLHGLAGDYAKE
                     KYSEKSLVATDIIENISEAYKYIL"
BASE COUNT          740 a          359 c          447 g          748 t
ORIGIN      
        1 atctccacta aaaacctaac aattcatcag aacttaacaa gcttctgttg taattggtac
       61 ttaaattatc aacaatatta gatatttttt atacttttgt aacaactaaa tatgtagcta
      121 tatgtataat tatgtaaagg tagcagccgc agtacccaat ctcgaagttg caaattgtta
      181 ttttaatcaa gaatcaattt gtgcaaaaat tatggaagca agacatcttg gaattgaggt
      241 attggttttt ccagaattat gcataacagg atatacttgt ggtgatttat tttttcaaca
      301 gacactacaa aaagccactt tagacgcttt acaacgaata ctttctgcaa cagaaagtat
      361 agatatgctt gttgctgtag gagctccatt gtgtattgat aatcagcttt ttaattgtgc
      421 tattgttttg tcaaaaggta agatagtagg aatagtacct aaaacgtata ttccaaatta
      481 taatgaattt tacgaacagc gttggttttc atctgcattt gatttgaaaa taaacacaat
      541 aaacatatgt agtcaggtaa taccaatagg taccgatttg ttattttcgt tgcacaatgg
      601 cactaaaatt ggtgttgaaa tttgtgaaga tttgtgggtt ccaattcctc ctagtgcagg
      661 tctttgtgtg tctggagcag atattattct taatttatcg gctagtaacg aaactgttgc
      721 caaaagacaa tatagaagaa atttagtcgt tcagcagtca gcacgttgta tgtgtgccta
      781 tgtatatgcg agtgctggag ggactgaatc aacaactgat ttagtgttta gtgggcactc
      841 tatgattgca gaaaatggcg ttttgctaaa agagaacgca caattaatag attcagatta
      901 tctactttcc tcacatgtag atatagataa gttacgtgta gatagatgca agtatacaac
      961 ctacaaagaa acccatcgag cctattttaa agaaattagg tgcataccat ctgatgttca
     1021 aagtaaacat aatgtatcgc ttgactatcc tattagccga aagccttttg tacctagtaa
     1081 tttacaccta cgcgataatc ggtgtcaaga aatagttgct atgcaagtag caggtttagt
     1141 caaacgttta gttcatacaa atgctccaac tgttgtaata ggtatatcag gaggattgga
     1201 ttctacttta gctcttttgg tgtgtgtgca agcttttgat gaactgaaaa aagacagaaa
     1261 aggtatactt gctgtaacca tgccagggtt tggtactacg aatagaacgt atgataatgc
     1321 tcttaccttg attcaggaac ttggagttac acgtagagtt attgatatta aaaaatcttg
     1381 ttatcagcat tttgaagaca tagatcatga tgagactatc catgatatta cttacgaaaa
     1441 tacacaagca cgggagcgaa ctaaactgtt aatgaattta gcgaataagc atagaggttt
     1501 tgttatcgga actggcgatt tgagcgaact tgctttgggt tggtgcacgt acaatggtga
     1561 tcatatgagt atgtatgctg tgaatgtaag tatacccaaa acgttaattc gtagtttagt
     1621 gttgaatata gcacatacac aagaggaaaa gattaaacag gttttgttag acatattaga
     1681 cactccagta agtccagaat tattaccaat aggaaaaaat ggcgaaatga tacaaaaaac
     1741 cgaagatagc gtaggtcctt acgaattgca tgatttcttt ttgttttatg tgatgcgtta
     1801 tgggtttagt ccaagtaaaa ttttatttct tgcaaagcac atattttcta aaaaagaaac
     1861 agaggaagat gtgttatttg acgatgaaac catacaaaaa tggcttattg tgttttataa
     1921 acggtttttt agtcaacaat ttaaacgtaa ctgtatgcct gatggtccta aagtagggag
     1981 tatttgtttg tctccgagag gcgattggcg tatgccaagt gatgccgttg cacacgaatg
     2041 gttaaaagaa atttcgggta atttataatt tgcgaatacg attttctata gtatatattt
     2101 gtatgcttcg cttatgttct caataatatc tgttgcaaca agtgattttt cggaatattt
     2161 ctctttagcg taatccccag ccaagccatg aaggtacact ccgagtaagg atgcctctat
     2221 tgcatcgtat ttttgtgccc gtaatcctgc tataattcct gtaagcacat ctccactacc
     2281 cgcagttgcc atac
//
LOCUS       DAOSMY010000258         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46967_length_2292_cov_3.175682, whole genome shotgun sequence.
ACCESSION   DAOSMY010000258 DAOSMY010000000
VERSION     DAOSMY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46967_length_2292_cov_3.175682"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLD76_05880"
     CDS             <1..402
                     /locus_tag="PLD76_05880"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOU27566.1"
                     /translation="HLIAEAAILDDNSTYSEPSTLLLIREQDIEKFFAQSLFPATSIG
                     IYAFLGTYNSTSKSYTFTSMADYFRMILKNEGDDANTLNDFVLVPVKTITNTSGTITS
                     LRHLYNPRGIRLRSGKNITSPMRIEITYSQF"
     gene            complement(454..705)
                     /locus_tag="PLD76_05885"
     CDS             complement(454..705)
                     /locus_tag="PLD76_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOU27567.1"
                     /translation="MVKTMTFNELRKIKDSLPDGTIRKIAQQLNITEETVRNYFGGHD
                     FDKGKAVVGVHIEPGPDGGVVTLDDTTILDLAMQEMARV"
     gene            complement(805..1533)
                     /locus_tag="PLD76_05890"
     CDS             complement(805..1533)
                     /locus_tag="PLD76_05890"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU27568.1"
                     /translation="MNYLEIVTNIFSSIVSWDTNMLLKLNSWNNLFFDGFMWITTSIL
                     VWLPLYISVIYIIIKNKKKESIIILFSLMLAIVLADQLASSVLKPLVERLRPTHNEDL
                     MPYIHTVNGYKGGIYGFVSSHAANAFAFVVFTAFLFRQFSYINLMLLWALLLSYSRIY
                     LGVHFPLDVIVGALLGGGIGYAAFVFYQLLKRIMPQYVYKKHKTNSRHYSPFSVKDLS
                     PFFFCFVITLFFSILFAYNFSFFE"
     gene            complement(1539..>2292)
                     /locus_tag="PLD76_05895"
     CDS             complement(1539..>2292)
                     /locus_tag="PLD76_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27569.1"
                     /translation="LLIQLFLFFHISLSLNATTAQVYDIKKANDLYVATSYAQAIEVY
                     EEISKQGYESATLYYNLGNAYYRINKIAPAILNYERALRLDPSNLDIQHNLKLAKAKT
                     VDKIVAQEKNIVEIWYNSIVFATHTNVWAYMSIIFFIVALCLVLMYMFLRVSWIRKIT
                     FFSSIIFFTFAICFFIFSYQQKKMVENQKEAIIFVPSSYVKSSPDQLGSDLFLLHEGT
                     KIIIKHQLGSWVQITTEDGNKGWLPVSTIQKI"
BASE COUNT          863 a          369 c          314 g          746 t
ORIGIN      
        1 catttaattg cagaagcagc catattagac gataatagta cttatagtga gccatctacc
       61 cttctgctga taagagaaca agatatagaa aaattctttg ctcaaagttt atttcctgcc
      121 acatccattg gcatctacgc attcttaggc acctataatt ccacatcaaa gagttataca
      181 tttacttcaa tggctgatta ctttagaatg atactgaaaa atgaggggga tgatgcaaat
      241 acgctaaacg acttcgtatt agtgccagta aaaaccatta caaatacaag tggtactatt
      301 acaagcttac gacatttgta taatcctaga gggataaggc tacgcagtgg caagaatata
      361 acatctccaa tgcgtataga aattacatat agccaatttt gattacaaaa aaaaagagga
      421 aaattcctct tttttttgta atgtcttaaa aaattaaact cgagccattt cttgcatagc
      481 taaatctaaa atggtagtat catctaatgt aactacacca ccatcaggac caggttcaat
      541 gtgtacacca acaacagctt tacctttatc gaaatcatgc cccccaaaat aatttctaac
      601 tgtttcttca gtaatgttta actgttgtgc aatttttctg attgttccat caggtaaact
      661 gtctttgatt tttctcaact cgttgaatgt catagttttt accataattt cacagtatta
      721 aattaataaa taaataatta tgtttttaaa gttgcaataa atatacgtca atattttata
      781 taaaaaaaat atttgacaca aaatttactc gaaaaaactg aaattatatg caaatagtat
      841 actaaagaag agggttataa caaaacagaa aaaaaatggg gataaatctt taacagaaaa
      901 tggagaataa tgtctactat ttgttttatg ttttttatag acatactgag gcattattct
      961 ttttaacaat tgatagaata caaaagctgc atacccaatg cctcctccta atagtgcacc
     1021 cacaatcaca tctaaaggaa aatgaactcc taaataaatg cgagaataag acagtaataa
     1081 tgcccaaagt aacattaaat ttatatacga aaattgtcta aatagaaacg ctgtaaaaac
     1141 tacaaaagca aacgcatttg ctgcgtgtga agatacaaat ccataaatac ctcctttata
     1201 tccatttact gtgtgaatat atggcattaa atcctcatta tgagtaggtc tcaatctttc
     1261 aacaagtggt tttaaaacag atgatgctaa ctgatcggct aatacaatag ctaacattaa
     1321 agaaaacaat ataataattg attctttctt tttatttttt ataattatat atataaccga
     1381 aatataaaga ggtaaccaga ctaaaataga ggttgtaatc cacataaaac cgtcaaaaaa
     1441 aagattgttc caactattta gtttcaacaa catatttgta tcccaactaa cgatagaaga
     1501 aaaaatgttt gttacaattt ctaaataatt catgtagttt atattttttg aatagttgaa
     1561 acaggcaacc atcctttatt tccatcttct gttgttattt gcacccaaga gcccaattga
     1621 tgtttaatga taatttttgt tccttcatgt aataagaata aatcactacc taattgatca
     1681 ggagaacttt ttacataaga agatggcaca aagataatag cttctttttg gttttctacc
     1741 attttctttt gttgatacga aaaaataaaa aagcatatcg caaatgtaaa aaatataatg
     1801 gaagaaaaaa acgttatttt tctaatccaa cttacacgta aaaacatata cattaataca
     1861 agacataatg ctacaataaa aaaaatgata ctcatataag cccacacatt tgtatgagta
     1921 gcaaatacga ttgaattgta ccaaatttca actatgttct tttcttgtgc aacaatttta
     1981 tctactgttt tagcttttgc aagtttcaag ttatgttgta tatctagatt ggatggatct
     2041 aaacgcaaag ctctttcata attaagaatg gctggggcaa ttttatttat acgataataa
     2101 gcatttccta agttataata caatgtggca gattcataac cttgcttact tatttcttcg
     2161 tatacctcaa tggcttgtgc atacgacgta gccacatata aatcattggc ttttttaata
     2221 tcatatacct gagcagtagt agcatttagg gacaatgata tatgaaaaaa aagaaacaat
     2281 tggattaaca aa
//
LOCUS       DAOSMY010000259         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_46989_length_2291_cov_3.201252, whole genome shotgun sequence.
ACCESSION   DAOSMY010000259 DAOSMY010000000
VERSION     DAOSMY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46989_length_2291_cov_3.201252"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1376
                     /locus_tag="PLD76_05900"
     CDS             114..1376
                     /locus_tag="PLD76_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOU27570.1"
                     /translation="MLHKVAVDIITIGDELLIGQIVDTNSAYIAKELAKNGFSTEHMY
                     SVSDKKEAIIEAIQRSFSQVDIVLLTGGLGPTKDDITKTVLCEYFQTKLVFSQDVYDD
                     VQELLSYRPNAMNGLNRQQAFVPQSCTVIRNKVGTAPIMWFEKNKKILVSMPGVPNEM
                     KWAMEHAVMDKLKRFFQLPTILHQHIIVYGYPESVLADTLTDWENNLPNSISLAYLPS
                     ISHVKLRMSAIAEDATLLSIQMQKQVELLKTILGKAIIALEDKPLEELLSNWLIDNNL
                     TLSVAESCTGGNIARLVTSISGSSAYFKGGVIAYANEIKEQVLAVSTQSLTEYGAVSQ
                     QVVEQMAKGVMQLTGSDVAIATSGIAGPSGGTENKPVGTVWVAVCNRHTVKSHCFSFG
                     GIRERVIERASYAGIIMLKELIEYTSLK"
     gene            complement(1351..>2291)
                     /locus_tag="PLD76_05905"
     CDS             complement(1351..>2291)
                     /locus_tag="PLD76_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOU27571.1"
                     /translation="ERIWGGQNLKHTLGKVFDNQFIGESWELSGLPNNQSVVANGMLI
                     GKTLTELIETYKHDLVGNIIYQRFGSSFPLLFKYLDACDNLSVQVHPSNEIAQKKHQC
                     DGKTEMWYVVSASDNAQIIAGVKDNIELKDIKQSIEKNTIEDLLHFHDAKQGDVFHIT
                     EGIIHALGKGTIVAEIQQSSDITYRVYDYNRKDQHGKSRDLHINDALDCIVLNDVNKT
                     HADFSETDIQQELVSCPFFTTNLLSITQPTSFNYQAIDSFVVYMSIEGDYVVETSFSS
                     LDVKKGDTILIPSSVKDIRIIPKDGGKALILETYIQ"
BASE COUNT          718 a          389 c          425 g          759 t
ORIGIN      
        1 aaaattatcg gcaaaatcat tgtctaattt acaaccacat ccagcatatg tatttgtgca
       61 ttattcgcat cctactttgt tgcaacgtat taacgcatta actcaaaagt gaaatgttac
      121 acaaagtagc tgttgatatt attaccattg gagatgagtt gttgataggg cagatagtcg
      181 acacaaattc agcatatata gctaaagagc ttgcgaagaa tggattctcc accgaacata
      241 tgtattccgt ttctgataaa aaagaagcca ttattgaagc tattcaacgt tctttcagtc
      301 aggtagatat tgttctttta acaggtggat tgggaccaac taaagatgat attactaaaa
      361 ccgtgttatg cgaatatttt caaactaaac tcgtttttag tcaagatgtg tatgatgatg
      421 tgcaagagtt actttcttat cgcccaaatg cgatgaatgg tttaaatcga caacaagctt
      481 ttgttcctca atcatgtact gttattcgta acaaagttgg tacagctcct attatgtggt
      541 ttgagaaaaa taaaaaaatt cttgtatcca tgcctggtgt gccaaatgaa atgaaatggg
      601 caatggaaca tgctgtaatg gataaattga aacgtttttt tcaacttcca actattcttc
      661 accaacatat aatagtgtat ggctatccag aatcagtact tgcggatacg cttacagatt
      721 gggagaataa tttgcccaat tcaatctctc tagcttatct tcctagtatt tctcatgtta
      781 aattacgaat gagtgccatt gcagaagatg caacgttact ctccattcag atgcaaaaac
      841 aagttgaatt gttaaaaaca attctgggaa aagcgatcat tgcattagaa gacaaacctc
      901 ttgaggaatt attaagcaat tggcttatag ataataatct tacactttct gttgctgaaa
      961 gttgtacagg gggcaatatt gcccgtttag taacatcaat atcaggtagt tctgcttatt
     1021 ttaaaggagg agttattgca tatgcaaatg aaataaaaga acaagtctta gccgtttcta
     1081 ctcaaagttt gacagaatat ggggctgtaa gtcagcaggt tgtagaacaa atggcaaaag
     1141 gggtgatgca attaacaggg agcgatgtag ctattgcaac atcaggaata gctggtccaa
     1201 gtggcggtac tgagaataaa ccagttggaa ccgtttgggt agcagtatgc aatcgacata
     1261 cagtaaaaag ccactgtttt tcgtttggtg gaatcagaga acgtgtgatc gaaagagcct
     1321 cctatgcagg gataattatg ttaaaagagc ttattgaata tacgtctcta aaataagtgc
     1381 ttttcctccg tccttaggga taatgcggat atcttttacc gatgatggta tgaggattgt
     1441 atctcctttt tttacgtcca aacttgaaaa ggatgtttct accacataat cgccttcaat
     1501 actcatatat accacaaacg aatcaatagc ctgatagttg aatgaagtag gttgagttat
     1561 tgaaagtaaa ttagttgtaa aaaatggaca gcttactagc tcttgttgga tgtctgtttc
     1621 tgaaaaatcg gcgtgcgttt tgttgacatc attcaataca atacaatcta aagcatcgtt
     1681 aatatgtaaa tctcttgatt ttccatgttg atcttttctg ttgtagtcat atacccgata
     1741 agtgatatcc gatgattgtt gtatttctgc tacaattgta cctttgccta atgcatggat
     1801 aatgccttct gtaatatgaa atacgtcacc ttgtttagca tcatgaaaat gcaataaatc
     1861 ttcaatagta ttcttttcta tgctttgttt tatatctttt agttcaatgt tatcttttac
     1921 ccctgctata atttgtgcat tatcagaagc agaaactaca taccacattt cagtttttcc
     1981 atcacattgg tgtttttttt gtgctatttc gttgctggga tgcacttgta cggataaatt
     2041 atcgcaagca tctaagtatt taaatagcaa agggaatgac gacccgaatc gttgatagat
     2101 gatattgcca accaaatcgt gtttatatgt ttctattagt tctgttaatg tttttccaat
     2161 taacatacca tttgccacaa ctgactggtt gttgggcaag ccagaaagtt cccaactttc
     2221 gccaataaat tgattatcaa aaactttacc aagagtatgt tttagatttt gaccacccca
     2281 aatacgttct t
//
LOCUS       DAOSMY010000260         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_47303_length_2279_cov_3.388040, whole genome shotgun sequence.
ACCESSION   DAOSMY010000260 DAOSMY010000000
VERSION     DAOSMY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47303_length_2279_cov_3.388040"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="leuB"
                     /locus_tag="PLD76_05910"
     CDS             complement(<1..971)
                     /gene="leuB"
                     /locus_tag="PLD76_05910"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOU27572.1"
                     /translation="MKLNIAVLPGDGIGPEIIKQGLDVTIAVCEKFGHELTYEYGLVG
                     ATAIDQVGNPYPDATHELCMKSNAVLFGAVGDPRFDNNPSAKVRPEQGLLAMRKKLGL
                     YGNIRPVTTFPSLIHKSPLRADLIDGADFVCIRELTGGMYFGRPQGRSEDGTVAYDTC
                     VYSRMEIERVLTLAYSYALKRRKKLTVVDKANVLATSRLWREVAKEMAQNYPDVETEF
                     MFVDNAAMRMIQWPKDFDVMVTENMFGDILTDEGSVITGSMGMLPSASIGEHTSVFEP
                     IHGSWPQAAGKDIANPLATILSAAMMFEYAFDLKEEAALIRKAVDAS"
     gene            complement(1045..1491)
                     /locus_tag="PLD76_05915"
     CDS             complement(1045..1491)
                     /locus_tag="PLD76_05915"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOU27573.1"
                     /translation="MNKQLTIALVAHDRRKADMVEWALHNAETLSQHKLVCTGTTGGL
                     IQEAFENKSIAADITRVNSGPMGGDAEIAAMVVKHQIDLAIFLIDDLNPQPHEADIQM
                     LLRQCRVHNVPIACNRYSADLMITSTLWDNPTYQPTEPKYVVFERK"
     gene            complement(1534..>2279)
                     /locus_tag="PLD76_05920"
     CDS             complement(1534..>2279)
                     /locus_tag="PLD76_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOU27574.1"
                     /translation="STNINEPELNRVSKLVEAYSGIRIPPNKPVVGDNVFTQCAGIHA
                     DGDAKKNLYFNDLLPERFGRIREYALGKTSGKANIRKNLEALGIELDEESMHKVTQRI
                     IELGDQKQVVTQDDLPYIVSDIVKEENIAENVRILNYSLSLAQGLKPIANILIEINGE
                     TYQEYATGDGQYDAFMKALWKIYDTLGKPHPILLDYLVTIPPGGRTDALVQTLITWDL
                     DGNVFKTHGLDGDQTAAAIKATLKMLNRI"
BASE COUNT          687 a          480 c          361 g          751 t
ORIGIN      
        1 attgaggcgt ctacagcttt gcgaattaaa gcagcttctt cttttaagtc aaaagcatat
       61 tcaaacatca tagcagcaga taaaatagtt gctaatggat ttgcaatgtc ttttccagca
      121 gcttggggcc acgatccatg aattggttca aatacagaag tatgttcgcc tatagaggct
      181 gaaggcaaca ttcccataga tccagttata acggaacctt cgtcagttaa aatatcacca
      241 aacatatttt cggttaccat cacatcaaaa tctttgggcc actgaatcat acgcatagcc
      301 gcattatcca caaacataaa ttctgtttct acatcagggt aattttgtgc catttcttta
      361 gctacttctc tccacaaacg cgaagttgcc aatacatttg ctttatctac aacagtcaat
      421 tttttcctac gttttaacgc atagctatat gccaacgtca acacacgttc aatttccata
      481 cgactataaa cgcaggtatc ataagctact gttccatctt cacttctacc ttgaggacga
      541 ccaaaataca taccacctgt taattctctg atacatacaa aatcggcacc atcaattaaa
      601 tccgcacgta atggagattt gtgaatcaac gacggaaacg ttgtaacagg acgaatattt
      661 ccatataaac caagtttttt acgcatagct agtaagcctt gttcgggacg tactttagca
      721 cttgggttgt tatcaaaacg aggatctcct actgctccaa aaagtacagc attggatttc
      781 atacataatt catgtgtagc atcaggataa ggattaccaa cttggtcaat ggcagtagct
      841 cccactaacc catactcata ggttaattcg tgtccaaatt tttcacaaac agcaatggtt
      901 acatctaacc cttgtttaat aatttctggt cctatgccat ctccaggcaa aactgcaatg
      961 tttaatttca ttttagtagt tattgtttat gttaaaagta tacttaatag agataattgg
     1021 ggattatttt ttgctaatgt tgtactattt ccgttcaaat acaacatatt ttggttcagt
     1081 tggctgataa gtaggattat cccacaacgt actagttatc atcaaatcag cactgtatct
     1141 attacaagca ataggcacat tatgcaccct acattgtcgc aaaagcattt gaatatcagc
     1201 ttcgtgaggt tgtggattta agtcatcaat caaaaatata gctaaatcta tttggtgttt
     1261 aacgaccata gcagcaatct ctgcatcacc acccattgga cctgaattaa ctcgtgtaat
     1321 atcagcagca attgatttat tttcaaaagc ttcttgtatt aaacctccag tagtacctgt
     1381 acacactaac ttatgttgag ataaagtttc ggcattatgc aacgcccatt ctaccatatc
     1441 tgcctttctt ctgtcgtgag ctaccaaagc aatggttagt tgtttattca ttttattact
     1501 gtttatttaa tttactccat tcatttgaag taattatata cgatttagca ttttcagtgt
     1561 agccttaata gcagcagccg tttggtcgcc atctagtcca tgtgttttaa acacatttcc
     1621 atccaaatcc caagttatta acgtttgtac caaagcatct gtgcgacctc caggaggaat
     1681 agttaccaaa taatccagca aaatgggatg aggttttcct aatgtatcgt aaattttcca
     1741 taatgccttc ataaatgcat cgtattgacc atcgccagtt gcatattctt gatatgtttc
     1801 tccatttatt tctatcaaaa tattagctat tggctttaat ccctgagcca gtgatagaga
     1861 ataattaagg atacgtacat tttcagcaat attttcttct tttacaatat ccgaaacaat
     1921 atatggcaaa tcgtcttgtg ttaccacttg tttttggtcg cccaactcaa tgatccgctg
     1981 agttacttta tgcatggact cttcatccaa ctcaatacct aaagcctcaa gattttttct
     2041 gatgttggct tttccagatg ttttaccaag agcgtattct ctaatgcgac caaagcgttc
     2101 gggcaataaa tcattaaaat ataaattctt ttttgcatca ccatcagcgt gaataccagc
     2161 acattgtgta aacacattat cgcccacaac aggtttgttt ggtggaatac ggatacccga
     2221 atatgcttct acaagcttac ttacacggtt taattctggc tcattaatat tggtcgata
//
LOCUS       DAOSMY010000261         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_49093_length_2218_cov_3.894591, whole genome shotgun sequence.
ACCESSION   DAOSMY010000261 DAOSMY010000000
VERSION     DAOSMY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49093_length_2218_cov_3.894591"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLD76_05925"
     CDS             complement(<1..990)
                     /locus_tag="PLD76_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27575.1"
                     /translation="MKKRFIVLIVGLLSYFVVSAQFSVTGVDVVEYPQSDIAAVFVVD
                     AFTDGVLSYTLPENKDVTWSYYDESGNKTIIKSEIQVNKTSIPLQNNSSLGYELDIQG
                     ELQYVWVFHYPDFEVQLDDIQIDTNTEDRCQFIQAQLFITANSMKYQGYNQQTMPYEV
                     VRTFELAYDSAYFSQTTYEVESVVRKIEQTSYVQLTSPLMNTLYTLSGDQFARAFNRT
                     KEISSAQVEAFAVQINPTASIQTRDGLNEKERDLSSSKTLRGSAPLEVEVLSYANVPA
                     ARYFDWCLSREADFSTCELQYIDKDFRYSFSQQIRYYVRLNVYNADATCKADTS"
     gene            1227..>2218
                     /locus_tag="PLD76_05930"
     CDS             1227..>2218
                     /locus_tag="PLD76_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU27576.1"
                     /translation="MRKLIVFLVASFITFFVIASNPNVSKQVTVNGIQYYQYTVQKSE
                     GFYAISTKFGVTQDDIILANPSTKEGLRVGQEILIPIKTEAKKENYLLHTVEKGETVF
                     SISQKYKISKEEIYSLNPETKEVLKIGSTIKIKSNATTENKPTNPSPTSIPQKTSVTD
                     KPIQQISDNKTENTYIEHRVRRRETLYSISELYDVEIDTIIKENPSVVNGLIVRSILR
                     IPMGKKAQQVEAITEKAFEEKPIAPIPQTSVVANKQDKKTHIKIAVLMPFELEATVRD
                     AGMDRFVEFYQGILIATDSLAQHGITIEVEAHDIGKIQNQLTKTLGIPSLKEVD"
BASE COUNT          797 a          406 c          358 g          657 t
ORIGIN      
        1 actcgtatca gctttacatg tagcatctgc attgtataca tttaaccgta cataataccg
       61 tatttgttgg gaaaacgaat accgaaaatc tttatcaata tactgtaatt cgcaggtaga
      121 aaaatctgcc tcacgcgaga ggcaccaatc aaaataacgt gcagcaggca catttgcata
      181 gcttagtact tccacttcca aaggagcaga cccacgcaaa gttttcgatg aagacaagtc
      241 cctttctttt tcatttaatc catcacgcgt ttggatactt gcagttgggt taatttgtac
      301 tgcaaatgcc tctacctgag cagatgaaat ttctttggta cgattaaatg cacgtgcaaa
      361 ttgatcacca gacaatgtat aaagtgtatt cataagagga gacgtaagtt gaacgtacga
      421 agtttgttct atttttctta cgactgactc tacctcataa gtagtttggg aaaaataagc
      481 agaatcgtat gctagttcaa atgttcttac tacttcgtat ggcatagttt gttgattgta
      541 cccttgatac ttcatagaat ttgccgtgat aaacaactgt gcctgaataa attgacacct
      601 atcctctgta ttagtatcta tctgtatatc atccaattga acttcaaaat cgggataatg
      661 aaatacccaa acatactgta attcgccctg aatgtcaagt tcgtagccaa gagatgaatt
      721 attttgtaac ggaatggaag ttttatttac ttgtatttcg gatttaataa tagttttatt
      781 tccagattca tcgtaataac tccatgtaac atctttgttc tcaggcaatg tataagataa
      841 tacaccatca gtaaatgcat ctacaacaaa tacagctgca atatcagact gaggatactc
      901 cactacatca acaccagtaa cactaaattg ggctgaaaca acaaaatacg aaagcaaacc
      961 aacaataagt acaataaacc tctttttcat tttataattg cttagttatg tttgaaaaaa
     1021 gcgagcattt actctttatt tgtaaaaagt caaaataata tgtcgttatt attttgttaa
     1081 actgattttt taaaaaatat tgtgtaaaga tagtattttt tatgctacta acaagtgttt
     1141 tcttccccta aaagacagta caatttgtag tttttatgta tctttgcctc attgtaacaa
     1201 ttttatatga aaaaataata gtcgttatgc gaaaactcat tgtgtttctg gttgctagtt
     1261 ttatcacttt ttttgtcatt gcctctaatc caaatgtttc taaacaagta acggtaaatg
     1321 gcatacaata ctaccaatat accgtacaaa aaagcgaggg attttatgcc atatccacaa
     1381 aatttggggt tacacaagat gacattattc tagcaaatcc atctacaaaa gagggactac
     1441 gtgtaggaca ggaaattctc atacctataa aaacggaagc aaaaaaagaa aattatctat
     1501 tacacaccgt ggaaaaaggg gaaaccgttt tttcaatatc acaaaaatac aaaataagca
     1561 aggaagaaat ttacagctta aatccagaga ccaaagaagt acttaaaatt ggttctacga
     1621 taaaaattaa gtctaatgct actactgaaa acaagcctac taacccttct cctacgagta
     1681 tcccacaaaa aacctccgtt acggataaac cgatacaaca aatatctgac aataaaacag
     1741 aaaacaccta tattgaacat agggttcgtc gtagagaaac gttatacagt atttctgagc
     1801 tctatgatgt agaaatagac accattatta aagaaaatcc aagcgttgtt aatgggttaa
     1861 ttgttcgttc cattcttcgt atcccaatgg gtaaaaaagc acaacaagta gaggctatta
     1921 cagaaaaagc ttttgaagaa aaaccgatag cacccatacc acaaacaagt gtagtagcaa
     1981 acaaacaaga caaaaaaaca cacatcaaga ttgctgtatt gatgccattt gaactagaag
     2041 ccacagtaag agatgcaggc atggatcgtt ttgttgaatt ttatcaaggc atactaattg
     2101 ccacagactc gctcgcacag catggtatca ccatagaggt tgaggcacat gatataggta
     2161 aaatacaaaa tcaactaact aaaacccttg gtataccctc gttgaaagag gtagatat
//
LOCUS       DAOSMY010000262         2205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_49461_length_2205_cov_3.566977, whole genome shotgun sequence.
ACCESSION   DAOSMY010000262 DAOSMY010000000
VERSION     DAOSMY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49461_length_2205_cov_3.566977"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..903
                     /locus_tag="PLD76_05935"
                     /pseudo
     CDS             271..903
                     /locus_tag="PLD76_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            1280..1831
                     /locus_tag="PLD76_05940"
     CDS             1280..1831
                     /locus_tag="PLD76_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOU27577.1"
                     /translation="MKRIGERIKKYRRIYNMALGDLATKVGITPSALSQIENAKAFPS
                     IITLKSIAENLHTTVGELIGENEASKHSPVYRAVDYKLLDNLDYKATIYHIPYEDVNK
                     QMDSFVIILKPQEKVENIFASHSGQRMCYVLQGNIEFTIDEKKVELKTGDSAFFHAKN
                     ISILQNKQQEDTKLLLVIAPASM"
     gene            1896..>2205
                     /locus_tag="PLD76_05945"
     CDS             1896..>2205
                     /locus_tag="PLD76_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27578.1"
                     /translation="MMCNREQKITKEKQKFPAIFLDRDGTLIEDVGYIKEPSAVSFFP
                     NTFADLIRLQEHFMLFIITNQSGIGNNLLTEKEVKIVNSYIETELKKQGRAKTIHKRE
                     F"
BASE COUNT          842 a          396 c          315 g          652 t
ORIGIN      
        1 aaagcattat ttctgatggt attctaatgc gattaatgac ttttccaaac gtactgatta
       61 cgtctcatca agctttcttt acacacgaag cacttacaca aattgccgaa acaacctatc
      121 aaaatatgct cgattttacg cacggtactt catcaactaa tcaattacta taaccatgat
      181 gactaacagt agtaacatcg aacttaaaag caagttgcat aacactacat gtaaagtgtc
      241 gcgttttaaa gagcaaataa aaaaactaaa ccacacaaac acaacaacta ttacgagcta
      301 attattttgc tcgaaggcag tggatttcat caaatagaat ctacccatta cctgattcaa
      361 gcccctgttt gttttgtttt gcaacctgga caaatgcatt attggcagtt tacttccata
      421 cccaaaggat atgtcatctt gtttagcaat gatgaatttg atgctctaca tgaaaaaaat
      481 gtatgcttat tgtataagca attagctttt catacgcaca tcaacatatc aaaagaaaca
      541 ctaccaattg ctttattaga agagctatac aaggtataca gcgaaccaaa tcatttttct
      601 caactcatca tacatgggtt attgcaatcg ttgtttgcca ctttattaat taccacacaa
      661 aaccacgacc tacaagctca atcaatgagt ttaccattcg aacagtttag caaattgatt
      721 caacaacaca tacatacaca tcacaaggta taccaacatg ctgcattatt aaacatgaca
      781 ccccaaaact tgaatgccat ctgccttaaa catggaaaaa cgaatgccgc aaaccacatt
      841 aacaatcacc tgcttttaga agccaaacga tacatcctgc atacggaaaa aaaccattgc
      901 tgaaatagct gacctactat cgtttagcga ttcatctcat tttgttaagt tctttaaaaa
      961 atacgaaggc attacacccg tgcaatttag acaacagttt tttcaatagt agcacacata
     1021 tggattcttt tatacatatt actacctaac aaatactggg tgtggtacaa caatcttgca
     1081 taacctattg ttttcaattt ttgcaaacat atgcacagta caaataaggt aggattacaa
     1141 agaagccctc atggcttgtt tcatgttatt ttacacacaa tagatttatt taaagtatgt
     1201 taatttaaaa ttaatatttt aagcgttact taattattta aactaaaata ctatctttgt
     1261 agtcaaataa tagtacacaa tgaaacgcat cggagaaaga ataaaaaaat acagacgcat
     1321 ctataatatg gcattaggag atttagctac aaaagttggt atcactccta gtgcattatc
     1381 tcaaatcgaa aatgcaaaag catttccatc tattattaca ctaaaatcga ttgcagaaaa
     1441 tctacatacc acagttggtg aattaattgg agagaatgag gcatccaaac attctccagt
     1501 atatagagca gtagattata aattacttga taatcttgac tacaaggcta ccatctatca
     1561 cattccatac gaagatgtta acaaacaaat ggatagtttt gtgataatct taaaaccaca
     1621 agaaaaagtt gaaaatatat ttgcatcaca cagcggacaa cgaatgtgtt atgtgttaca
     1681 aggaaatata gaatttacca tcgatgagaa aaaagtagaa ttgaaaacag gtgatagtgc
     1741 attttttcac gcgaaaaaca tctctatatt acaaaataag caacaagagg atacgaaact
     1801 tttattagta atagccccag caagtatgta aaactacatt tatctgatat ttttattgat
     1861 gaataaaaat ttgtattaaa aaaattttta tctttgtgat gtgtaatagg gaacagaaga
     1921 taacaaaaga aaagcaaaaa tttcctgcaa tatttcttga cagagatggc actcttatag
     1981 aagatgtggg atacataaaa gaaccaagcg cggtttcttt ttttccaaat acattcgctg
     2041 atttaatccg tttacaagaa cactttatgc tgtttattat taccaatcaa tctggtattg
     2101 gaaataattt actgacagaa aaagaagtaa aaatagtaaa ctcatatatt gaaacagaat
     2161 taaaaaaaca aggacgagca aagacaatac ataagcgaga gtttc
//
LOCUS       DAOSMY010000263         2205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_49462_length_2205_cov_3.518140, whole genome shotgun sequence.
ACCESSION   DAOSMY010000263 DAOSMY010000000
VERSION     DAOSMY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49462_length_2205_cov_3.518140"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="PLD76_05950"
     CDS             <1..2039
                     /locus_tag="PLD76_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27579.1"
                     /translation="STSQNNKQIQLFNQQEAGVNTLVQSMPQHTYFFNHSHITQLGVY
                     KKNVQKLLSFPQQQAAKQQHDQLTTSTGENPSLFFDRHFDKEIAFGYAPYTSKFDQNA
                     FVLIKIKDEPEAIARLQRMANESSPVATSTKNGNISIIQWQSNGFAGSVFGSQYTFPE
                     EYIAIYKGILYIAPSRNMLVYLLQQKERNRTLHRSSTYASNTQSMYQFANNTLYIDIP
                     SILRRMQSVFTPEKIVWINSTRALWNNFETLILQAENKRNDLQFQQLVVNYTKKTEID
                     ILEEKQADMLVENEQPNIEDTSTIHTTQSEKTETTTPESASPTPAKKTRITALKTWEL
                     ALTKPIATSPQLVINQTTGLHEIMVQDTDNFLYLITSEGKLVWRIRIAGRIISDIHQV
                     DMYRNKRLQMVFNTAEKLYIIDRSGKNVNNFPIIFATKATAGVAVFDYNNNGDYRFFI
                     PLANKRIALYKKDGSQPSDWKFKQSTELVNKPIQYFRSNGMDYLVASTNKKSYFLHRR
                     GNERLIPEKPVISSSNNNFSLIYTGGIEKFVTTDKNGNIVLINPNKKVDKVQLRTWSS
                     QHAFTTFTHQKTNYYVFVDNKRIEIYDEEFNSVMLYEDIQTKKPSIMVWENLLATYNE
                     KSKEIVLVDLIARQVVANDIKTDKNLFYIGQLKPYKKTCVVVSEKNKVIHYVLN"
BASE COUNT          833 a          439 c          362 g          571 t
ORIGIN      
        1 cgagcactag ccaaaacaac aaacagatac aactatttaa ccaacaagaa gctggagtta
       61 ataccttggt acaaagcatg cctcaacaca cctatttttt taatcattca catatcaccc
      121 aattaggggt atacaaaaaa aacgtacaaa aattactctc gtttcctcaa cagcaagcag
      181 caaagcaaca acatgatcaa ctcaccacaa gtacgggtga aaatccgagt ttgttttttg
      241 atcgtcattt tgacaaagag atcgctttcg gctatgctcc ctatacgagc aaatttgacc
      301 aaaacgcatt tgtgctgatt aaaataaaag atgaacccga agcaattgcc cgattacaac
      361 gaatggcgaa tgaaagttct cccgttgcta cctctacaaa aaatggaaat atttcaatta
      421 tacaatggca aagcaatgga tttgctggaa gtgtgtttgg ctctcagtat acttttcccg
      481 aagaatatat cgccatttac aaaggaattt tatacattgc tccatcgcgt aatatgcttg
      541 tgtacttatt acaacaaaaa gaacgtaaca gaaccttgca tcgttcgtct acctatgcta
      601 gcaatacaca atcaatgtac cagtttgcca acaataccct ttacatcgac attccttcta
      661 tactaagacg tatgcaatcg gtatttacgc ccgaaaaaat tgtgtggata aacagtacac
      721 gtgcactgtg gaataatttt gaaacactca tcctgcaagc agaaaataaa cgaaatgatt
      781 tgcaatttca acaactagtg gtcaactaca cgaaaaaaac agaaatagac atattggaag
      841 aaaaacaagc ggatatgtta gtagagaatg agcaaccaaa tatagaagac actagtacta
      901 tacataccac ccaatcagaa aagactgaaa caacaacccc cgaatcggca agccctaccc
      961 ctgctaaaaa aacaaggata acagctctca aaacatggga gttagctctt actaaaccaa
     1021 tagctactag ccctcaactg gtcataaacc aaacaactgg actacacgaa ataatggtgc
     1081 aagacactga taacttttta tatttaatta ccagcgaagg aaaactcgta tggcgtattc
     1141 gcattgctgg acgcattatt agcgatatcc atcaagtgga tatgtacaga aacaaaaggt
     1201 tgcaaatggt gtttaatacc gccgaaaaac tatacatcat cgaccgtagt ggtaagaatg
     1261 taaacaattt ccctataatt tttgcgacaa aagctactgc tggagtggct gtattcgact
     1321 ataacaacaa tggcgattat cgctttttta ttccacttgc caacaaaaga attgccctgt
     1381 acaaaaaaga tggctcacaa ccaagcgact ggaaatttaa gcaaagcaca gaactcgtaa
     1441 acaaacctat acaatatttt agaagtaatg gcatggatta tttagtggca agtacgaata
     1501 aaaaaagcta tttcttacac cgcagaggca atgaacgatt aattccggaa aaacctgtaa
     1561 taagctcctc aaacaataat ttctcactca tatatactgg aggaatagaa aaatttgtta
     1621 ctacagataa aaatggaaat atagtactta ttaatccaaa taagaaagtg gataaggtgc
     1681 aactacgcac ttggagtagc caacatgcct ttacaacatt tacacaccaa aaaacaaact
     1741 attatgtatt tgtagataat aaacgcatcg aaatatacga cgaagagttt aacagcgtta
     1801 tgctgtacga agacatacag acaaaaaaac caagcattat ggtgtgggaa aatttacttg
     1861 ctacctacaa cgaaaaatca aaagaaatcg tattagtaga tttaatagct cgacaagtag
     1921 tagcgaacga tataaaaacg gacaaaaatc tattctacat cggacaatta aaaccataca
     1981 aaaaaacctg tgtagtagta agcgaaaaaa ataaggtaat acattatgta ttgaactaac
     2041 aaggcttttt taattattct tcttgtatat tcgatgtata acggaataaa tttgtggatc
     2101 aaatatgacc ttcgctatcc aattatgaat caccggttca ccgtagctct taacctcgca
     2161 ctacacgcta agtgcatggc gtttcttttc tgatgctttt gttgt
//
LOCUS       DAOSMY010000264         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_49850_length_2193_cov_3.351263, whole genome shotgun sequence.
ACCESSION   DAOSMY010000264 DAOSMY010000000
VERSION     DAOSMY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49850_length_2193_cov_3.351263"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1819
                     /locus_tag="PLD76_05955"
     CDS             206..1819
                     /locus_tag="PLD76_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU27580.1"
                     /translation="MITVSNLAIQFGKRTLFQEVNLKFTAGNCYGIIGANGAGKSTFL
                     RLLSGDLDPTKGSIHFGSNERLSVLKQDHHEFDEFTVLDTVLMGHTKLWDIKNEKDAI
                     YMKEDFSDEDGIRASELEEAFAELDGWNAESNAASLLSGLGIKEDLHYSLMKDISGKE
                     KVRILLARALFGNPDNLLLDEPTNDLDIDTINWLENYLANYENTVLVVSHDRHFLDAV
                     STHIVDIDYGKIQLVPGNYSFWYESSQLALRQQQAQNKKAEEKKKELEEFIRRFSANV
                     AKSKQATSRRKMLEKLNVEEIQPSTRKYPGIIFTPDREPGNMVLEVKGLSHSMEGQVL
                     FKDVNFNVEKGDKIVFLSKDPRAMTAFFEIINGNIKPDSGTFNWGVTITSAYLPLNNV
                     DFFNTDLNLVDWLGQFSDDTTESFIRGYLGRMLFSGEEIYKKAGVLSGGEKMRCMISR
                     MMLKNANVMVLDSPTNHLDLESIQAFNNSLKSFKGNILMSSHDHEFIQTVCNRVIELT
                     PNGIIDKVIEYDDYLDDMHVKTQREKLYK"
     gene            1983..>2193
                     /locus_tag="PLD76_05960"
     CDS             1983..>2193
                     /locus_tag="PLD76_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27581.1"
                     /translation="MNKATYSILCIVLLLTSCNLRDKEVIDPDYFFKNDSNLSLHTVV
                     ITDKATRLTFSILTESPTQIKIDSTA"
BASE COUNT          756 a          378 c          400 g          659 t
ORIGIN      
        1 taattgttgt agggatttat tatgtatcaa gccattttat aggtcatact cacgatcacc
       61 accaccacac cgaagaatgt acgcatgacc acgagcattg agctattatc tctatttgat
      121 tttctgacag ctacactttt cttttaaatc taaagaaaat tgcctatctt tgtggtcgca
      181 aaaataaaac aaacttacaa tagacatgat tacagtttca aatttggcca ttcaatttgg
      241 caaacgcact ttatttcaag aagttaattt aaaatttact gccggaaact gttatggcat
      301 aataggtgct aatggtgcag gaaaatcaac cttcttacgt ttgttaagtg gcgatttaga
      361 cccaacaaaa ggctccattc atttcggttc aaacgaacgt ttatctgtat taaaacaaga
      421 ccaccacgaa tttgatgaat ttactgtact tgacaccgtt ttaatgggac acaccaagtt
      481 gtgggatatt aaaaacgaga aagatgctat ttacatgaaa gaagattttt cggacgaaga
      541 tggtataaga gcatctgaac tagaagaggc ttttgccgaa ctcgacggat ggaacgccga
      601 aagcaatgca gcatcgctac tgagtggttt gggtataaaa gaagacttgc actactcgct
      661 aatgaaagac attagtggaa aagaaaaagt acgtatattg ttggcaaggg ctttgtttgg
      721 aaacccagac aacctattgc ttgatgagcc taccaacgat ttggatattg acaccatcaa
      781 ctggttagaa aattatttag caaactacga aaataccgtt ttggtagtat cgcacgaccg
      841 tcacttttta gatgctgtaa gtacccatat tgtggatatt gattatggca aaatacaatt
      901 agtaccaggt aactattctt tctggtacga atctagtcag ctggctttgc gtcagcaaca
      961 agcacaaaac aagaaagcgg aagaaaagaa aaaggaattg gaagagttta ttcgtcgttt
     1021 tagtgccaac gtggctaaat ccaaacaagc gactagccgt agaaaaatgc ttgaaaaact
     1081 aaatgttgaa gaaattcaac cctctacccg caaataccct ggtatcattt ttacacccga
     1141 tcgcgaacct ggcaatatgg tactggaagt gaaaggactt tcgcatagca tggaaggtca
     1201 agtattgttt aaggatgtaa actttaacgt agaaaaaggg gataaaattg tatttttatc
     1261 caaagaccct agggctatga ctgcgttttt cgagattatc aatggcaata taaaaccaga
     1321 cagtggaaca tttaactggg gagtaaccat tacctcagcc tatttaccgc ttaataatgt
     1381 tgattttttt aatactgatt tgaatttagt agattggttg ggacaatttt ctgacgatac
     1441 taccgaatca tttatacgtg gctatttggg cagaatgttg ttttctggcg aagaaattta
     1501 taaaaaagca ggcgtattat caggaggaga aaaaatgcgt tgtatgattt cgcgtatgat
     1561 gctgaaaaat gccaatgtta tggtgctcga ctctcctacc aaccacttgg atttggaatc
     1621 gattcaagca tttaacaact cgctaaaatc ctttaaggga aatatcctga tgtcgtctca
     1681 cgaccatgag tttattcaaa cagtatgtaa ccgtgtaatt gaactaacgc ccaatggcat
     1741 tattgataaa gttatagaat acgatgatta cttagacgat atgcacgtaa aaactcaacg
     1801 agaaaaatta tacaaataac aatttgttgt aacaaaaaat aaaacctcct atactacttt
     1861 gtttaggagg tttttttatt attttaataa tcgatatact attattactc ttttcacgtt
     1921 tatagaagta agttgaaaga cacaaacaat aatttttttt tgaaatttaa tactaaaact
     1981 ctatgaacaa agcaacatat tccatattgt gtattgtcct attattaact agttgtaacc
     2041 tacgagataa agaagtaata gaccctgatt atttttttaa aaacgatagc aatctttcat
     2101 tacacacagt agtaattaca gataaagcaa cacgtctaac tttttcaata ctcactgagt
     2161 cgccaacaca aataaaaatt gattcaacgg cgt
//
LOCUS       DAOSMY010000265         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_49854_length_2193_cov_2.919083, whole genome shotgun sequence.
ACCESSION   DAOSMY010000265 DAOSMY010000000
VERSION     DAOSMY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49854_length_2193_cov_2.919083"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLD76_05965"
     CDS             <1..220
                     /locus_tag="PLD76_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOU27582.1"
                     /translation="EQGNDLRGKAEIIIAKHRNGSTGDVFLRFKQDFIKFTNLASEES
                     YAEFQSKSTDSQEHDFLQANTYSNPSPF"
     gene            236..1813
                     /locus_tag="PLD76_05970"
     CDS             236..1813
                     /locus_tag="PLD76_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU27583.1"
                     /translation="MQNLLLLGDEAIAQGAIDGGISGVYAYPGTPSTEITTYIQETTD
                     KREIRSDWSVNEKTAMEAAMGLSYVGKRALVCMKHVGLNVAADAFINAAITGINGGIV
                     VLVADDPSMHSSQNEQDSRFYGKFALIPILEPSNQQEAYDMAYTGFELSEKLGYPILM
                     RITTRLAHSRSGIIRKPQKQQRPISLPTNRKQFILLPAIARQRYSALLAAQSEFEEQA
                     NNSLYNEYIDGVNKKMGIIACGLTFNYLMENFPNGCEYPIVKIGQYPLPRKKIERLID
                     ECESILVIEDGYPLVEELLKGYTGKGIQVKGKLDGTLPRQGELNPNNVGQAFGFTLEK
                     DITIPEVVKMRPPSLCKGCGHIDLYESLVEVLKDYPTARVFSDIGCYTLGALPPYTAI
                     DTCLDMGASITMAKGAADGGLHPSVAVIGDSTFTHSGITGLIDCVNDNSNVTIIIVDN
                     ETTAMTGGQTSSGTGKLEAICQAVGVDENHIHVCEPLKKNIDYIVQLIKKEISYTGVS
                     VIIPRRECIQTLKRKKK"
     gene            1817..>2193
                     /locus_tag="PLD76_05975"
     CDS             1817..>2193
                     /locus_tag="PLD76_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU27584.1"
                     /translation="MKIDIILSGVGGQGILSIAAVIGQAALENKHYMKQSEVHGMSQR
                     GGDVQANLRISDKPIFSDLIPLQQADLILSLEPMEALRYMPYVKEAGWIVTNETPFKN
                     ISNYPNEADIEDEIKKHKNHILI"
BASE COUNT          769 a          376 c          425 g          623 t
ORIGIN      
        1 cgaacaagga aacgatttaa gaggtaaagc cgaaattatt attgccaagc atcgaaatgg
       61 ttctacaggc gatgtatttt tacgtttcaa acaagacttt attaaattta ccaatcttgc
      121 atcagaagaa tcgtatgctg aatttcaaag taaaagtaca gatagtcaag aacatgattt
      181 tttgcaagca aatacgtatt ctaatccttc accattttaa actatattta tattcatgca
      241 aaatttatta ttattaggag acgaagcaat agcacaagga gctattgatg gtggtatttc
      301 aggtgtgtat gcttatccag gcaccccatc taccgaaata acaacctata ttcaagaaac
      361 aaccgacaaa agagagatac gtagcgattg gtcggtaaat gaaaaaacag ctatggaggc
      421 tgctatgggt ttatcttatg tagggaaacg agccttagta tgtatgaaac atgttggctt
      481 gaatgttgct gcagatgcct ttattaatgc tgccattaca ggaataaatg gaggaatagt
      541 agttttagta gctgatgatc catccatgca ttcgtctcaa aacgaacaag attcacgttt
      601 ttatggtaaa tttgcactta ttccaattct tgaacctagt aatcaacaag aagcatatga
      661 tatggcttat actggttttg aactttcaga aaaattaggc tatccaatat taatgcgtat
      721 taccacacga ttagctcatt cccgatcagg cattatacgt aaaccacaaa agcaacaacg
      781 ccctatatcg ttacccacaa atagaaaaca gtttatttta ctacctgcca ttgcaagaca
      841 acgttatagt gctttgttgg cagcacaaag tgaatttgaa gaacaagcaa ataactcact
      901 atacaatgaa tatattgatg gagtgaacaa aaaaatggga ataatagcct gtgggttaac
      961 atttaattac ctgatggaga atttccccaa cggatgtgaa tatcccattg taaaaattgg
     1021 acaatatcca ttgcctcgta aaaaaattga aaggttaata gacgaatgcg aaagcatatt
     1081 ggtaattgaa gatggatatc ccctagtgga agaattacta aaaggctata caggaaaggg
     1141 catacaggta aaaggaaaac ttgatggcac gctacctcga caaggagaac tcaaccccaa
     1201 taatgtgggg caagcttttg ggtttactct agaaaaagac attactatac cagaagtagt
     1261 aaaaatgcgt ccaccatcgc tttgcaaagg atgtgggcat attgatttgt acgaatcatt
     1321 ggtagaagtc ctcaaagact atcctactgc tcgtgttttt agcgacattg gttgttatac
     1381 tttaggagca ttgcctccct atactgccat tgatacttgt ttggatatgg gagcatctat
     1441 tacgatggca aaaggggcag ccgatggtgg tttgcatcca tctgttgcag taattggtga
     1501 ttctacattt acccactcag gtattactgg actaatagat tgtgtcaatg acaatagcaa
     1561 tgttaccatc attattgttg ataacgaaac aacggcaatg actggtggac aaacatcgtc
     1621 tgggacagga aaacttgaag caatttgtca agccgtagga gttgacgaaa atcatataca
     1681 tgtatgcgaa ccactaaaga aaaatattga ctacatagta caacttatca aaaaagaaat
     1741 cagctatact ggagtgtccg taattattcc tcgcagagag tgtatacaaa ctttaaaacg
     1801 taaaaagaaa taaactatga aaatagacat cattttatca ggtgtaggtg gtcaaggaat
     1861 tttatctatt gctgccgtta ttggacaagc tgctcttgaa aacaagcatt atatgaaaca
     1921 atccgaagta catggtatga gccagcgtgg tggagatgta caagccaatt tacgtatttc
     1981 ggataagcca attttctctg acttaatacc tttgcaacaa gctgacctta ttttgtctct
     2041 cgaaccgatg gaagcattgc gttatatgcc ttatgtaaaa gaagctggat ggatagtaac
     2101 taatgaaact ccattcaaaa atattagcaa ttatccaaac gaagctgata ttgaagatga
     2161 aataaagaaa cacaagaatc atattttaat taa
//
LOCUS       DAOSMY010000266         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_50097_length_2185_cov_3.100000, whole genome shotgun sequence.
ACCESSION   DAOSMY010000266 DAOSMY010000000
VERSION     DAOSMY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50097_length_2185_cov_3.100000"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..527
                     /locus_tag="PLD76_05980"
     CDS             147..527
                     /locus_tag="PLD76_05980"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOU27585.1"
                     /translation="MAQSYLGVWEQINNDGQKGALVRIEKSSRANTYVGTIIHVFRYP
                     QDILCTHCKGELHNKPVVGMQFMYDMEYKKGELVNGKIIDVKTGKTYYTKLSYNKQDD
                     ALLMRASVDASGFIGETQVWKRYK"
     gene            complement(540..1784)
                     /locus_tag="PLD76_05985"
     CDS             complement(540..1784)
                     /locus_tag="PLD76_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU27586.1"
                     /translation="MNVALFIARKLQFGNKEKKKDANPSIRIAIIGIALGLAIMLLAV
                     AIVTGFKHEIQGKVIGFGSHIQVTSLSNNTTYETPPIIFPDSLIQSIKQIDGVKHVQR
                     FATKPAIIKTDDEFQGIVIKGIDNQFNWEFFSKNLIEGETIVFNDSATSNDVIVSQDI
                     AKTLQLKVNDRFLCYFVKDGIRVRRFTVKGIYNSNFSEFDKLFILADLRHIQRLNGWE
                     ENEITGVEILVTDYRKLDDITDAVFMQTANRFEEKGNTYYTRSIKQLQPQIFGWLDLL
                     DMNVWVILILMLTVAGFSMISGLLILILERTNMIGILKALGGKNWFIRKIFLYQAFFL
                     VGKGMLWGNILGISLALLQQQFNIITLDPLTYYVNTVPINLNLWYIVFVNALCLFFSL
                     LVLLAPSHMIAKIEPSKAIRFE"
     gene            complement(1813..>2185)
                     /locus_tag="PLD76_05990"
     CDS             complement(1813..>2185)
                     /locus_tag="PLD76_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="catalyzes the formation of oxalozcetate and
                     L-glutamate from L-aspartate and 2-oxoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU27587.1"
                     /translation="AEASIDTPKEYLEEMYNEYIERRNFLIEGLNKIPGVYSPLPMGA
                     FYTVARLPVDDADAFCSWILDEFNYEGQTVMMAPASGFYTGTTAGKNEVRLAYVLKKE
                     ELAKALVVLAKALEAYPGRTI"
BASE COUNT          712 a          403 c          362 g          708 t
ORIGIN      
        1 tttttgagta gaaattgata atgttcttaa taatttatag ttttgtaact agtaaaattt
       61 ctaactagta gtgtatgtcg tatccatttg taggtttaaa caaaatatat gtccttttcg
      121 tgtttacact atgcagtgta tcaactatgg ctcaatctta tcttggtgtt tgggaacaaa
      181 taaataatga tggacaaaaa ggagctttgg tgcgtataga aaaatcatct cgagcaaaca
      241 cctatgttgg aactattatt catgtttttc gctatccaca agatatttta tgtacccatt
      301 gtaaaggcga attgcataac aaaccagttg taggcatgca atttatgtac gatatggaat
      361 ataaaaaagg agagctcgta aatggtaaga ttattgatgt aaaaacagga aaaacatatt
      421 acactaaact atcttacaat aaacaagatg atgctctgct aatgagagct tcggtcgatg
      481 catctggttt tattggcgaa acacaggttt ggaaacgcta taaatagtct tcatattcat
      541 tattcgaacc gaatagcttt tgatggctca attttggcaa tcatatgcga tggagctaat
      601 aacaccaaaa gagaaaagaa taagcaaaga gcattcacga atactatgta ccacagattg
      661 agattgatag ggacggtatt tacataataa gtaagaggat cgagggtaat gatattgaat
      721 tgttgttgta gcaaagccaa actaatcccc aatatatttc cccaaagcat gcctttacct
      781 actaagaaga acgcttgata taaaaatatt ttacgaataa accagttttt acctcccaaa
      841 gctttcaata tgccaatcat attggtacgt tccaagatta gaattaacaa gcccgatatc
      901 atactaaaac cagccaccgt aagcatcagt attaaaatta cccacacgtt catatctagc
      961 aaatcgagcc aaccaaatat ctgaggttgc aattgtttaa tgctacgagt gtagtaggta
     1021 ttcccttttt cctcaaaacg attcgccgtt tgcataaata cagcatccgt aatatcatcg
     1081 agctttcgat aatccgtaac caatatttct acgccagtaa tctcattttc ttcccaacca
     1141 tttaatcgct gtatatgacg taaatcagct agtataaaca gtttatcaaa ttcgctaaaa
     1201 ttagaattat atattccttt taccgtaaaa cgccttaccc gaataccatc tttcacaaaa
     1261 taacatagga atcgatcatt taccttcaat tgtaatgttt tggcaatatc ttgcgataca
     1321 attacatcat ttgatgtagc actgtcgtta aaaacaattg tttctccctc aattaagttt
     1381 ttagaaaaaa actcccaatt aaactggttg tcaatacctt ttataacaat accttggaat
     1441 tcgtcatcgg ttttaataat agcaggtttg gtagcaaaac gttgcacatg ttttactcca
     1501 tctatttgtt taattgattg gataagagaa tcgggaaaga taatcggagg tgtttcgtag
     1561 gtggtgttat tgcttaaact cgttacttgg atatgagaac caaatccaat aactttgcct
     1621 tgtatttcgt gtttaaatcc agtaacaata gctacagcta agagcatgat tgccaagccc
     1681 aatgcaatgc caataatggc aatacggata gatggatttg cgtctttttt cttttcctta
     1741 ttgccaaatt gtaatttgcg agctatgaat aatgctacat tcatacacta ttgtttcgtt
     1801 tcatatacct tgttaaattg ttctacctgg atatgcctct aacgctttag ccaataccac
     1861 aagagctttt gctaattctt cttttttcag tacatatgcc aaacgaactt catttttgcc
     1921 agccgtcgtg cctgtataaa aacctgaagc aggtgccatc attacggttt gaccttcgta
     1981 gttgaactca tctaaaatcc atgagcaaaa agcgtctgca tcatcaactg gcaacctggc
     2041 tactgtatag aaagcaccca ttggaagcgg agaatatacg cctggaattt tatttaagcc
     2101 ttcaatcaag aaatttctgc gttcgatgta ttcattatac atttcctcta agtattcttt
     2161 tggcgtatca atagaagctt cggct
//
LOCUS       DAOSMY010000267         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_50206_length_2182_cov_2.521392, whole genome shotgun sequence.
ACCESSION   DAOSMY010000267 DAOSMY010000000
VERSION     DAOSMY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50206_length_2182_cov_2.521392"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLD76_05995"
     CDS             complement(<1..410)
                     /locus_tag="PLD76_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU27588.1"
                     /translation="MRYYIIAGEASGDLHASNLVKSIFQQDNQAVIRGFGGDLMQNEG
                     VQLVKHYRELAFMGFIPVLLNIRTIFRNLSFCQQDITDFKPDVVILIDYPGFNLKIAK
                     FVKLHLKLPVFYYIPPKIWAWKEYRIKAIKKYID"
     gene            complement(421..708)
                     /locus_tag="PLD76_06000"
     CDS             complement(421..708)
                     /locus_tag="PLD76_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27589.1"
                     /translation="MKQKFNHFLWGFIPGFLFPVLLFLVTWGSIYKGEFTFWDSVVRM
                     YGTHLMQQYILFCMLPNLLYIFFAYKTDRLKTASGVIVALVPYLSLLFMNI"
     gene            complement(692..1468)
                     /gene="surE"
                     /locus_tag="PLD76_06005"
     CDS             complement(692..1468)
                     /gene="surE"
                     /locus_tag="PLD76_06005"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU27590.1"
                     /translation="MKNRPLILVTNDDGVDARGIHSLIEAVSSLGEVVVVAPDGPRSA
                     QSNAITVNSPLRFWKHLEKDGLTVYRCNGTPTDCIKLGLCNILDEKPDIIVSGINHGS
                     NSSASVMYSGTMGGVFEGCRAGIPSVGFSLCNYDPRADFTIAMQYAYMISETVLVEGL
                     PTHTCLNVNVPPIQDVKGYKLCRQADGYWHEKFVKREDPYGNNYYWLTGNYVNNEPQA
                     TDTDEAMMAQGYVSVVPCKIDLTDYDALEELKRWNYEAKV"
     gene            complement(1540..>2182)
                     /locus_tag="PLD76_06010"
     CDS             complement(1540..>2182)
                     /locus_tag="PLD76_06010"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU27591.1"
                     /translation="KTNKKNGLAAAKVQLKNFVNEMSSLPSGSAELKTFLEQKMDVDL
                     FLRAYAVNVMVGMWDDYWYNTNNFYFYFDTNGKFYFIPYDYDNTLGTSSGMNSGKQDM
                     LNWGALDDSRLLMKKIMSITAFKNQYKAYIKELANANNDLFDTNKSIARIKQWHAMIG
                     NYIVNDTKEDMLIEDKPASWGNAGFYRLFTGDDKGGNAGDANFFKTKIKSITW"
BASE COUNT          722 a          433 c          308 g          719 t
ORIGIN      
        1 ttatcaatat actttttgat agccttgata cggtattcct tccatgccca tatttttggt
       61 gggatatagt aaaaaacagg taattttaga tgtagtttta caaatttggc tattttgaga
      121 ttaaaaccag gatagtcaat taaaataact acgtcgggtt taaaatcagt aatatcttgt
      181 tggcaaaaac ttaggtttcg gaatatagta cgaatattta ataatacagg aataaagccc
      241 ataaacgcca attcacgata gtgttttaca agctgtacac cttcattttg catcaagtcg
      301 ccaccaaaac cacgtataac tgcctgatta tcttgttgaa agattgattt tactaaattt
      361 gacgcatgta aatcccccga tgcttcgcct gcaataatat aatacctcat ggtgtaacta
      421 ttagatattc ataaacaaca aactcaaata cggaacaaga gctacaatta caccacttgc
      481 tgttttcaat ctgtctgttt tgtaggcaaa gaagatgtat aataaatttg gtaacatgca
      541 aaacaaaata tattgttgca tcaaatgggt gccatacata cgcacgacac tatcccaaaa
      601 agtaaattct cctttgtaaa tacttcccca agtaaccaaa aataatagca ctggaaataa
      661 aaaaccagga ataaatcccc ataaaaaatg attaaacttt tgcttcataa ttccatcgtt
      721 ttagttcttc taatgcatca taatccgtca aatctatttt gcatggtact accgatacat
      781 aaccttgtgc catcatagcc tcatcggtat cggttgcttg tggctcattg tttacataat
      841 tacctgtaag ccaatagtaa ttattaccat aaggatcttc tctttttaca aatttttcgt
      901 gccagtatcc atctgcctga cgacataatt tatatccttt cacatcttga ataggtggca
      961 catttacatt taagcaggta tgtgtaggca atccttctac caatacggtt tcggaaatca
     1021 tgtaggcata ttgcattgct atcgtaaaat cggcacgtgg atcgtagtta cacaacgaaa
     1081 aaccaacaga aggaatacca gccctacaac cttcaaatac tccacccata gtgcctgaat
     1141 acatcacact cgcagaagaa ttagaaccat ggttaatacc cgaaacaata atatctggtt
     1201 tttcatctaa tatattacac aaccctagtt taatgcaatc tgtaggagtt ccattacaac
     1261 gatacactgt caatccatct ttttctaggt gtttccaaaa acgtaatgga gaattgacag
     1321 taatggcatt tgattgagcc gaacgtggtc catcaggtgc taccaccaca acttccccta
     1381 aagagcttac cgcttcaatc agtgaatgta tacctcttgc atctactccg tcatcatttg
     1441 ttactaatat taatggtcta tttttcatat gtattactat tttgttgtat gcacaaatgt
     1501 aagaataagt aacaagaata cgaaattaat aaaccataat taccacgtaa tactcttaat
     1561 ttttgttttg aaaaaattag catcaccagc attacctccc ttatcatcac ctgtaaataa
     1621 gcgataaaaa ccagcattac cccaactggc aggtttatct tctattagca tatcttcttt
     1681 cgtgtcattt acaatatagt taccaatcat tgcatgccat tgtttaatgc gagcaatact
     1741 tttattggta tcaaataaat cattatttgc atttgctagt tccttgatat aggctttgta
     1801 ttggttttta aatgcagtaa tagacattat ctttttcatt aataaacgac tgtcgtctaa
     1861 tgctccccaa ttaagcatat cctgttttcc tgagttcatt ccactagaag tacccaaggt
     1921 attgtcataa tcgtaaggta tgaaataaaa tttgccattg gtatcaaaat aaaagtaaaa
     1981 attatttgta ttataccaat aatcgtccca catgcccacc ataacgttta cagcgtaagc
     2041 tcttaaaaac aaatcaacat ccattttttg ttccaaaaat gtttttaatt ctgcactacc
     2101 acttggcaaa gaagacattt cattaacaaa gttttttaat tgtactttgg ctgcagctaa
     2161 accattcttt ttatttgttt tc
//
LOCUS       DAOSMY010000268         2172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_50533_length_2172_cov_2.747284, whole genome shotgun sequence.
ACCESSION   DAOSMY010000268 DAOSMY010000000
VERSION     DAOSMY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50533_length_2172_cov_2.747284"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..980
                     /locus_tag="PLD76_06015"
     CDS             150..980
                     /locus_tag="PLD76_06015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU27592.1"
                     /translation="MKLTIVTINYNNATGLKKTMDSVFSQTDTDFEYIIVDGASTDTS
                     ISIIKEYEHSNIQHFHWISEPDKGIYNAMNKGIQKATGEYIYFLNSGDWFADTKVVEN
                     IFSAPRKGDVLYGNYYYVTDTKSTLYTYPETLTNGWFYAGGLCHQAVFVKTEQQKKHL
                     FEEKYRFVADWKVLFTLFVLEKKRFEYLNFPISYYTLDGFSSKPENIDALNNERLAIF
                     KELILPVDAKTEIDMAVKLLKLIDSPLTVYESKKKKTLTFSGIKAHILFNIARVLHKI
                     "
     gene            1016..2005
                     /locus_tag="PLD76_06020"
     CDS             1016..2005
                     /locus_tag="PLD76_06020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27593.1"
                     /translation="MSQLQTVPLVSIICPLYNKIDFIAETIQSVIDQSYTHWQFIIVD
                     DGSTDGSYEFVKSLNHSNIVLLQNDSGIKGPSAARNIGIEKATGEYTIFLDADDILLP
                     HCLEQRVREMMQDNKDFIVFQMNSFGTPVKEKTTFYSEDYLSDFIANNWKWTITGPIW
                     RTSSLKSLGGFDIRFRILEDPELHTRALCAGFSFTVLYNSEADCLYRQFENKKINLSL
                     TVESYYLFLYKLLAEENVLEKIGKQYQSAFLLSRKRAYNNVLVYFTPTNRDIDTLKKS
                     LSQIDALYFDKKLISKTKVWFSWALLHAFFIWKKIPILRNRVWLFKKLNTKLL"
BASE COUNT          782 a          319 c          352 g          719 t
ORIGIN      
        1 atattacata gtgcaagata ctaatcctat ccttgtaaag aaaagaatca ttttttcgaa
       61 tatgtatatc acaaaaaatg aaataagacg attgttgtag gcaatgaatg agagtactct
      121 agaagaggat gaacaccaaa agcataaaaa tgaaattaac cattgttacg ataaactaca
      181 ataacgctac aggtttaaag aaaaccatgg atagcgtttt ttcgcaaaca gataccgatt
      241 ttgaatacat cattgtagat ggagcaagta cagacacaag tatctccatt ataaaagaat
      301 acgagcattc gaacattcaa cattttcatt ggataagcga accagataaa ggcatataca
      361 atgcaatgaa caaaggtatc cagaaagcaa ctggagagta tatttatttc cttaattctg
      421 gcgattggtt tgccgatacc aaggtggtag aaaatatatt ttcagcacca cgtaaaggag
      481 atgtattgta tggtaattat tattatgtaa cagatactaa atcaacgctt tatacctacc
      541 cagaaacctt aaccaatggg tggttctatg ctggaggact atgtcatcag gcggtatttg
      601 taaaaacaga acagcaaaaa aaacacttat ttgaagaaaa atatcgtttt gtagccgatt
      661 ggaaagtact ttttacattg tttgtgttgg aaaaaaaacg gtttgagtat cttaattttc
      721 ctatctccta ttacacatta gatggcttta gctcaaaacc cgaaaatatt gatgcactca
      781 ataatgaaag attggcgata tttaaagaat taatcttgcc tgttgatgca aaaactgaaa
      841 tagatatggc tgtgaagctg ttaaagttaa tagattctcc actaaccgtg tatgaatcta
      901 aaaagaaaaa aacactaact ttctcaggca ttaaagccca catattattc aatatagcca
      961 gagtgcttca taaaatttaa tattcccttt atattaattt attaaaaaga aagagatgtc
     1021 ccaattacaa actgtaccat tagtgagtat tatttgtcca ttgtataata aaattgattt
     1081 tattgcagaa accattcaat cagttataga ccaaagctat acccattggc aatttattat
     1141 agtggatgat ggatcgacag atggttctta tgagtttgtg aaatcattaa atcattcaaa
     1201 cattgtactc ctacaaaatg attctggcat aaaaggacca tcagcagcta gaaatattgg
     1261 tattgaaaaa gctacaggag agtatactat ttttttggat gctgatgaca ttttgcttcc
     1321 tcattgtttg gagcaacgtg ttcgagagat gatgcaagac aataaggatt ttattgtttt
     1381 tcagatgaat agttttggta cccctgtaaa agaaaaaaca accttttata gcgaagatta
     1441 tttaagtgat tttatcgcta ataattggaa atggacaatt actggtccta tttggagaac
     1501 ttcttctctc aaaagtttag gaggttttga tattcgtttt agaattctcg aagatccaga
     1561 attacataca agagccttat gtgcaggttt ttcatttaca gtattataca atagtgaggc
     1621 tgattgttta tacagacaat ttgaaaataa aaaaatcaat ttatcgttaa ccgttgaatc
     1681 atattattta tttttatata aactattagc cgaagaaaat gtgttagaga aaatagggaa
     1741 acagtatcaa tcagcctttt tgttgtcgcg aaaacgtgcc tataacaatg ttttggtata
     1801 ttttactcca actaatagag atatagatac cttaaaaaaa tctttatcac aaatcgacgc
     1861 tctctacttc gataaaaaac taatcagtaa aaccaaagtt tggtttagtt gggcactatt
     1921 gcatgctttt tttatttgga aaaaaatacc gatattgcga aacagagtat ggctatttaa
     1981 aaagttaaat acaaaattgc tttaagcttt aatatttact acctttgcac tgttttactt
     2041 atttatttta cacaaatact taaactaaga tgctttttaa ttcaattgat tttgccatat
     2101 ttttaccaat agtatttctg ttttattggt ttgtttttcg taataactta aagcttcaaa
     2161 atctctttat tg
//
LOCUS       DAOSMY010000269         2139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_51633_length_2139_cov_3.032630, whole genome shotgun sequence.
ACCESSION   DAOSMY010000269 DAOSMY010000000
VERSION     DAOSMY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51633_length_2139_cov_3.032630"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="PLD76_06025"
     CDS             <1..90
                     /locus_tag="PLD76_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOU27594.1"
                     /translation="KSRKGFALGAVLAAEFTYGKQGFLGMNDL"
     gene            115..1542
                     /gene="lepB"
                     /locus_tag="PLD76_06030"
     CDS             115..1542
                     /gene="lepB"
                     /locus_tag="PLD76_06030"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU27595.1"
                     /translation="MSDIHNEKKIGRFDVSLIQWIKAFVVCLLYVLFIIWVGNFWWLL
                     LLPIFFDIYITKFIPWSFWKKSKNKYVLKIAEWTDAIVFALIAVYFINLFLFQNYKIP
                     TSSLEKSLLVGDFLLVSKVSFGPRVPNTPLSFPLAQHTLPILNTKSYIEYPQWEYKRL
                     KGLDTIKHLDIVVFNFPAGDTVTTKVSNPDYYTLCHDYGREYVHNNKNLFGDIIYRPV
                     DRRENYVKRCVGLPGDTLQIIEHQVYINGVAQEEVEGTQLSYWVTTDGRKISENQFKR
                     IGVSKDDQQFLINNLPNGDNLLDYCGYERNSEGKHNPVYRLPLTKEALNKVKNLPNVI
                     KIEIDNGLISGQVFPFGYKKWTRDNYGPIYIPRKGATIALNLDNLPVYERVIRNYEGN
                     TLEIMDSTIYINAVATTNYTFKMNYYWLMGDNRHNSADSRYWGFVPEDHVVGKPLFVW
                     LSLDKDASLLESIRWERLFKFVHKE"
     gene            1546..>2139
                     /locus_tag="PLD76_06035"
     CDS             1546..>2139
                     /locus_tag="PLD76_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOU27596.1"
                     /translation="MSGVVLTSTYLGNIQFYSKLVHFSEIWIEQHCHYTKQTYRNRCK
                     IATANGVMALSIPTIKPATEKCKTKDIRIDNSQHWQQIHWRALEAGYNSSPFFEYYKD
                     DFAPFYTNKFEFLVDYTFQLQSLVIALLELDCKVQLTDAYTTYDDALDYRDALSAKKE
                     IEDNDFVISSYYQVFENKFGFIPNLSILDLLFNMGNES"
BASE COUNT          762 a          320 c          397 g          660 t
ORIGIN      
        1 aaaagccgca aagggtttgc attgggtgcg gttttggctg ctgagtttac gtatggcaaa
       61 caaggattct taggaatgaa tgatttataa taattttaaa caaagataaa aaagatgagc
      121 gatatacata acgaaaaaaa aatagggcgt tttgatgttt cactaataca atggataaaa
      181 gcgtttgttg tatgcttgct atacgtacta tttattatct gggtaggaaa tttttggtgg
      241 ttattactac ttcctatatt ttttgatatc tatatcacga agtttattcc ttggagtttt
      301 tggaaaaaat cgaaaaataa atatgtcctt aaaattgctg aatggacaga tgctatcgta
      361 tttgcattga ttgcggtgta ttttatcaac ttatttttat tccaaaatta taaaattcct
      421 acctcatcat tggaaaaatc attgttggta ggcgattttt tgttagtaag caaagtaagc
      481 tttggacccc gagttccaaa cacgccactt tcttttccat tggcacaaca cacattacct
      541 atactcaata ccaaatcgta tattgaatac cctcagtggg aatataaacg attgaaaggg
      601 ctagatacaa ttaaacattt agatattgta gtatttaatt ttcctgcggg cgacacagta
      661 acaacaaaag tatcaaatcc agattactat acattatgtc atgattacgg aagagaatat
      721 gtacacaata ataaaaattt gtttggagac atcatctatc gcccagtaga taggcgagaa
      781 aactatgtaa aaagatgcgt aggattacct ggcgacacat tacaaataat tgaacaccaa
      841 gtatatataa atggagttgc ccaagaagaa gtagaaggca cccagttaag ttattgggtt
      901 acgactgacg gtagaaaaat ttcggaaaac caatttaaac gtataggggt aagcaaagat
      961 gaccaacaat tcttaatcaa taacctgcca aatggtgata acttgctgga ttattgtggc
     1021 tatgaaagaa atagtgaagg caaacacaat ccagtataca gattaccatt aaccaaagag
     1081 gctttaaaca aggtaaaaaa tttaccgaat gtaatcaaaa tagaaattga taatgggtta
     1141 atttcaggtc aagtattccc atttggttat aaaaaatgga cccgcgataa ttatggacct
     1201 atttatattc ctcgcaaagg ggctaccatt gcattaaatt tggataattt acccgtatat
     1261 gaacgtgtaa ttcgcaatta cgaaggcaac actttagaaa ttatggatag tacaatttat
     1321 ataaatgctg ttgcaacgac taattacacc tttaagatga attattattg gttaatggga
     1381 gataaccgcc acaattcagc agattctcgt tattggggat ttgtgccaga agatcatgtg
     1441 gtgggaaaac cactatttgt atggttatca ttagacaaag atgcatcgtt gctagaaagc
     1501 attagatggg aaagattatt taaatttgtg cataaagaat aagaaatgag cggtgtagta
     1561 ctcacatcca cgtatttagg gaatattcag ttttacagta aattggtaca cttttctgaa
     1621 atttggatag aacaacattg ccactataca aaacaaacct atcgaaatag gtgtaaaatt
     1681 gctactgcaa acggagttat ggcgttatca ataccaacca taaagcctgc aacagaaaaa
     1741 tgcaagacaa aagatatccg catagataat agccagcatt ggcaacaaat acactggagg
     1801 gctttagaag ctgggtataa ttcgagtcca ttttttgagt attacaaaga tgattttgct
     1861 cctttttata ctaacaagtt tgaattctta gtagactaca cctttcaatt acaatcgttg
     1921 gttattgcat tattggaact cgactgtaag gtgcaattga cagatgcata taccacatac
     1981 gatgatgcgt tagattacag agatgcacta tctgcaaaaa aagaaataga agataacgat
     2041 tttgtaatta gttcgtatta tcaagtattt gaaaataaat ttggttttat tcctaattta
     2101 agtattcttg atttgttgtt taatatggga aatgaatcc
//
LOCUS       DAOSMY010000270         2138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_51661_length_2138_cov_3.544407, whole genome shotgun sequence.
ACCESSION   DAOSMY010000270 DAOSMY010000000
VERSION     DAOSMY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51661_length_2138_cov_3.544407"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLD76_06040"
     CDS             <1..463
                     /locus_tag="PLD76_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOU27597.1"
                     /translation="REFDHLTKEVEYQTLEIEHCEKNIREFTVAIDVKKEEKAKALAQ
                     LEDMNANLKEKKEELEEIVSETRQEEEKLRDDAKQLEKLIEDRLLQAFKRIRKNARNG
                     LAVVYIQRDACGGCFNKIPPQRQLDIRLRKKIIVCEHCGRILVDSELANAE"
     gene            547..>2138
                     /locus_tag="PLD76_06045"
     CDS             547..>2138
                     /locus_tag="PLD76_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOU27598.1"
                     /translation="MGEIHLVSDLAIILIAAGIVTLLFKRLKQPMVLGYIVAGFLVGP
                     YFDVLPTIIEKESVHQWSEIGVIFLLFALGLEFSFKKLMHVGSSALITAGTEVVTMFS
                     LGTLVGYLLHWTMIESFMLGGMLSMSSTTIIIKAFDDLGIRKHKFTSIVFGTLVVEDI
                     VAIVMMVLVSTAALSKEFAGIDMLYSIVKLAFFLVLWFLVGIYVFPSFFQKARKWMND
                     ENLLIISIGFCFGMVAMATYTGFSAALGAFIMGSIFAETVEGEHIEKLSKGIKDLFGA
                     IFFVSVGMMVDPSVLVEYWFPVLLLTLSTIFGKAFFSFLGVLLSGQDLRTAIQSGMSL
                     AQIGEFAFIIATLGYTLGIMSPHIYPIIVAVSVISTFTTPYMIRLANPLYEWIYPRLS
                     SNTKETLAKYVTYSNRVTTSSDWKQLLRAFAVQVIGYTVICVGIILFMFHFVHPFLLE
                     KLNVWMPNVLINLFQALLTIAFLFPFLKGLMVNRNRKQDIFLKLWNENKVTRGGLMSL
                     LLLRVFIAVVLVTFVLYSCFTH"
BASE COUNT          624 a          280 c          436 g          798 t
ORIGIN      
        1 tcgagagttt gaccatttga caaaagaggt tgaatatcaa acacttgaga ttgaacattg
       61 tgaaaaaaat atacgtgaat tcacagttgc cattgatgta aaaaaagaag aaaaagcaaa
      121 agctcttgct cagctagaag atatgaatgc taatttgaaa gagaaaaaag aagaattaga
      181 agaaattgta tcagaaacta gacaagaaga agaaaagctt cgagatgatg ctaagcagtt
      241 ggaaaaattg atagaagatc gtttattgca agcatttaaa cgtattcgta aaaatgcacg
      301 taatggttta gctgtggtat atattcaacg tgatgcatgt ggcggttgtt ttaataaaat
      361 tccacctcaa agacagttgg atattcgcct acgtaaaaaa attattgtat gcgaacattg
      421 cggacgtatt ttagttgatt ccgaactagc taacgctgaa taaattctat ttttatagtt
      481 aatagcctga tatatgtgtc aggctatttt ttttattttt atcctactta atgtcattat
      541 ttttgtatgg gggaaataca tttagtttct gatttagcca ttatcttaat tgctgctggt
      601 attgttacgt tgctttttaa gcgacttaag caacctatgg ttttgggata tattgtggct
      661 ggatttttag tgggacctta ttttgatgtt ttacccacaa ttatagaaaa ggagagtgtg
      721 catcagtggt ctgaaattgg tgtgattttt cttttgtttg cattggggct tgagtttagt
      781 tttaaaaaac taatgcatgt tggcagttca gctcttatta cagcaggaac tgaagttgta
      841 acgatgtttt cgttgggcac attggtaggg tatttacttc attggacaat gattgaaagt
      901 tttatgcttg gtggtatgct ttcaatgtca tcaactacca tcattatcaa ggcgtttgac
      961 gatttaggaa ttcgcaagca taaatttacc tctattgttt ttggtacctt agtcgttgaa
     1021 gatatagttg ccattgttat gatggtttta gtatcaacag ctgctttaag taaagaattt
     1081 gcaggtattg acatgttata cagcattgtt aaattagcct ttttcttggt attgtggttt
     1141 ttggttggga tttatgtttt tccctctttt tttcaaaaag ctcgaaagtg gatgaacgat
     1201 gaaaatttgt tgattatttc cattgggttt tgttttggga tggtagcaat ggctacttat
     1261 acaggtttct cagccgcatt gggagcattt ataatggggt caatttttgc cgaaacagtg
     1321 gaaggagagc atattgaaaa attgtctaaa ggcattaaag atttatttgg tgctatattt
     1381 tttgtatctg ttggtatgat ggttgaccca tctgtattag ttgaatattg gtttcctgtg
     1441 ttattgctta cgttatcgac gatctttggc aaagcctttt tctccttttt gggggtgtta
     1501 ctttctggtc aagatttacg tacagccatt cagtcaggta tgagtttagc tcaaatcgga
     1561 gagtttgcgt ttattatagc aacattgggt tatactttag gcataatgag cccgcatata
     1621 tatcctatta tagtagcagt atcggttatt tctaccttta caactcctta catgatacga
     1681 ttagctaatc ctttatatga gtggatatat ccgcgtttat caagcaatac caaagaaaca
     1741 ttagccaaat atgtaactta ttcaaatagg gtgacgacta gcagcgattg gaaacaatta
     1801 ttgagagcgt ttgctgtgca agtaattggc tataccgtga tttgtgttgg tatcatttta
     1861 tttatgtttc attttgttca tccttttcta ctagaaaaat taaatgtctg gatgcctaat
     1921 gtattgatta atctttttca ggctttgttg actattgcat ttttatttcc ttttttaaaa
     1981 ggattaatgg taaacagaaa tagaaaacaa gatatatttt taaaactatg gaatgaaaat
     2041 aaagttacac gaggaggatt gatgtcatta ttattgttgc gtgtttttat agctgttgta
     2101 ttagtaactt ttgtattata ttcttgtttt acacatag
//
LOCUS       DAOSMY010000271         2138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_51670_length_2138_cov_2.634662, whole genome shotgun sequence.
ACCESSION   DAOSMY010000271 DAOSMY010000000
VERSION     DAOSMY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51670_length_2138_cov_2.634662"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLD76_06050"
     CDS             complement(<1..1056)
                     /locus_tag="PLD76_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU27599.1"
                     /translation="MTSIYEVRQRFKLLFSSLAILIAISSFVVTNSLVQKLSDEEKSK
                     MQIWANATKELINMDEYCDISFVQQVIEANTTIPVILVTQEDSVISYKNIPERLQESR
                     KLQQLVEEYKKSNTPIEIVFSDTERQYLYYGNSLLLIQLSYFPFVQLIAVFIFLVFVI
                     WALLSTKKAEQNEVWVGLSKETAHQLGTPISSLMAWTEIFKSKYIEDSMFGEMEKDVN
                     RLRTIAERFSQIGSKTDLEIVNLNQVIHNAVDYLRTRISKKITLSIQENEVLYCELNV
                     SLFEWVIENLCKNAADAMSGDGIIKIQLSQKGKEIRIDVIDNGKGISKHNSRKIFNPG
                     FTTKKRGWGLGLSLSRRI"
     gene            complement(1134..2055)
                     /gene="rsgA"
                     /locus_tag="PLD76_06055"
                     /pseudo
     CDS             complement(1134..2055)
                     /gene="rsgA"
                     /locus_tag="PLD76_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
BASE COUNT          691 a          423 c          279 g          745 t
ORIGIN      
        1 aatacggcgg gatagactca agcccagccc ccatcctctt tttttagttg taaaaccagg
       61 attaaagatt ttacgactat tatgttttga tatccccttg ccattatcta ttacatctat
      121 tctaatttct ttaccttttt gcgacaattg aattttaatg atgccatcac cactcatagc
      181 atctgcggca ttcttgcaca aattttcaat cacccactca aataatgata cattcaattc
      241 acaatagagt acttcattct cttgtataga aagagtgatt tttttggaga tgcgtgttcg
      301 taaataatct acagcattgt gtataacttg atttaagttc actatttcca aatctgtttt
      361 ggagcctatt tgcgagaaac gttctgcaat ggtacgtaat ctatttacat ccttttccat
      421 ttcaccaaac atcgaatctt caatgtattt cgacttaaaa atttctgtcc atgccattaa
      481 agaggatatt ggagtcccta gttgatgtgc cgtttcttta gacaatccca cccatacttc
      541 attttgttct gcttttttgg tgcttaaaag agcccatata acaaatacta aaaatataaa
      601 tactgctatt aattgaacaa aaggaaaata cgacaattga ataagcaaca gtgaatttcc
      661 atagtacaaa tattgtcgtt ctgtatcact aaatacaatt tcgataggcg tattgctttt
      721 tttatattct tcaactaatt gctgtaattt tctagattcc tgcaaacgct ctggaatatt
      781 tttatacgaa ataacactgt cctcttgtgt aacaagtata actgggatag tagtatttgc
      841 ctctattact tgttgtacaa atgaaatatc acaatactca tccatattaa tcaactcttt
      901 cgtagcattt gcccatattt gcattttaga cttttcttca tctgaaagct tttgcaccaa
      961 actgttagtt acaacaaacg agcttattgc aatcaatatc gcaagacttg aaaaaagcag
     1021 tttaaagcgt tgccttactt cgtaaatgga agtcataaga atgtatattt tgtgtacgtt
     1081 aatgtaaaca accaatacat ataacgtata ttatataaaa aaagtatatc cgtttatcta
     1141 tatttcgcat cttcagtcat atcctgtaaa atactcaaat aactctggta tcgagattga
     1201 cttatacgaa attctcctac agcttttata acagcacaat ctggctcttg ttgatgtaaa
     1261 caattattat atttgcattg atgcgagtat tcaaaaatat ctctaaaata atgtgctacc
     1321 tcgtgctttt ttatatctac aataccaaag cccttaatac ccggagtatc aataatgtaa
     1381 cctcctgaca atgtatatat gggaattagt tgttgtatgc attcccttat tatgcatttc
     1441 tgagataagc ccaattttaa catctaatgt agggtcaatt tggtgtatca aacttgattt
     1501 accaacaccc gaattaccag aaaaaagaga aatatttcct cttaaacact ctttcaactc
     1561 ttcgataccc tgcatactta ctactgaagt ttctatacaa cgatacccaa tatgttgata
     1621 caactgtttt aaagcatcca aataattacg ctcattttct atatacaaat caaccttatt
     1681 aaacacaatt atcacaggca catggtacgc atcggcactc accaaaaatc tatcaataaa
     1741 acctgtagaa gtttctggat aatttaccgt aaccactaat atagcttggt caatatttgc
     1801 cccaataata tgcgaatgtt tcgacaaatt tgatgacttt cgcacaatat aattctttcg
     1861 atcgcatata tcataaataa atgaatgacc atttgtttgt aaatcaacta gcacattatc
     1921 cccaatggct ataggattag tgcttctaat tcccttcagc ctaaaattcc cctttattct
     1981 acactcaaca gatactcctg tttgtagctg aacaagataa atatttccat tattttgaat
     2041 tacaagacct tgcattcttc tattttaaaa agcttctgta gctgtaatat aaaattccac
     2101 attcccataa ttagttctgg cgatgtctgc ccgtaaat
//
LOCUS       DAOSMY010000272         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_51873_length_2131_cov_2.925819, whole genome shotgun sequence.
ACCESSION   DAOSMY010000272 DAOSMY010000000
VERSION     DAOSMY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51873_length_2131_cov_2.925819"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..964)
                     /gene="pyrB"
                     /locus_tag="PLD76_06060"
     CDS             complement(56..964)
                     /gene="pyrB"
                     /locus_tag="PLD76_06060"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOU27600.1"
                     /translation="MKNKSLVSISDFSKEDILRILVEAEKFENNPNTRILEGKVIAML
                     FFEPSTRTRLSFETAVNRLGGRVIGISDVATSSSTKGETLNDTIKMVSGYADLIIMRH
                     HIDGAARYASEVSAVPIINAGDGSNQHPTQTMLDLYSIKKTQATLENLQISLVGDLKY
                     GRAVHSLVMAMSHFNPSFHFVSPPELQIPDSYKLLCNKQGISYKQYVEISEAINKADI
                     LYMTRVQRERFTDPVEYEKVKNTYVLKNAMLENSKQNLRILHPLPRVNEIDMDVDTNP
                     KAYYFEQARNGVYVRQAIICSILGLL"
     gene            complement(1178..>2131)
                     /locus_tag="PLD76_06065"
     CDS             complement(1178..>2131)
                     /locus_tag="PLD76_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOU27601.1"
                     /translation="NGRTQYGLVVCASVDDYMQEKIKKHELTRRDKEEDRMKHVRINN
                     ANIEPVFLAYPNNKEIDAIVSKTIMQKATYDFVAPDGFGHHFWVINDTNDIHRITKLF
                     ANIPSLYIADGHHRSAAAALVGNEKKLQNPHHKGDEEYNYFLAVCFPDNQLNIMDYNR
                     VVKDLNGLSDSDFLTALEKNFVVEDKGTAIYHPNTLHNFSLYLSGKWYSLTAKPGTYN
                     DNDPIGVLDVTISSNLILDELLGLKDLRSDKRIDFVGGIRGLEELKKRVDSGEMKVAL
                     ALYPVSMKQLIDIADTGNIMPPKTTWFEPKLRSGLVIHKLV"
BASE COUNT          624 a          390 c          350 g          767 t
ORIGIN      
        1 taccaccaat tcgtgattga tatttgtttt catacggata attttattta tttacctaaa
       61 gaagtcctaa tattgaacag ataattgcct gacgaacata tactccgttg cgtgcttgct
      121 caaaataata cgctttcgga tttgtatcca catccatatc aatttcgttt acacgaggta
      181 gcgggtgtaa aatccgtaag ttttgtttac tgttttctaa catagcattt tttagtacat
      241 atgtgttttt tactttctcg tattctacag ggtctgtaaa acgttcacgt tgcactctcg
      301 tcatatataa aatatcagcc ttgtttattg cttccgatat ttccacatac tgtttgtaag
      361 aaattccttg tttattacat agcagtttat acgaatctgg tatttgtaac tctggaggtg
      421 aaacaaagtg aaaggaaggg ttaaaatggc tcatagccat tactaaagaa tgtacagccc
      481 taccatattt taggtcgcca accaaactta tttgaaggtt ttccaatgtg gcttgcgttt
      541 ttttgatgga gtacaaatca agcattgttt gtgttggatg ctgattagat ccatctcctg
      601 cattgataat cggtacggca gatacttctg aagcgtatcg tgcagcaccg tctatatgat
      661 gtcgcataat tattaaatct gcgtagccac ttaccatttt gatggtgtcg tttagcgttt
      721 cgcctttcgt agagcttgaa gtggctacat ctgaaatgcc aataacacgc cctccaagtc
      781 tgtttaccgc tgtttcaaaa cttaatcttg tacgcgtaga tggttcaaaa aacaacattg
      841 ctattacttt accttccaaa atgcgggtat tcggattgtt ctcaaatttt tctgcttcta
      901 caagaatacg caatatgtct tctttggaga agtctgaaat agacacgagg cttttgtttt
      961 tcatcgaaac catttttttt gtgtaataaa taaactaaaa tccaaataaa ataaagccac
     1021 tacaactttc gttatactgg ctttattgtt atggtaaatt acatgttttc atacataaat
     1081 tttctaaagt agatatacaa aaagattcat ccatttcttt gctaatataa tatcatacta
     1141 caaatgtata aaatatcgtt gatatttcat ctgtttgtta aactaattta tgaattacca
     1201 atccagaacg taattttggt tcaaaccaag ttgttttagg tggcatgata tttcctgtgt
     1261 cagcaatatc aatcagttgt ttcatagaaa cgggatacaa cgctaatgct actttcattt
     1321 cgccactatc aacacgtttc tttaattctt ctaaaccacg tattcctcct acaaaatcaa
     1381 tacgtttatc gcttctaagg tctttgagcc ctagtagttc atctagtata aggttagagg
     1441 aaatagttac atccaacaca cctatcggat cattgtcgtt atacgtgcca ggtttggctg
     1501 tgagagagta ccatttccca cttaaataaa gagagaaatt atgtaatgta ttgggatgat
     1561 aaattgctgt acctttatct tctactacaa agtttttttc taatgctgtc aaaaaatcac
     1621 tatcacttaa tccattcaag tcttttacta cacggttgta atccataata ttcagttgat
     1681 tatcagggaa acaaacggct aagaaatagt tgtattcctc gtctccttta tgatggggat
     1741 tttggagttt tttttcgttg cctactaaag cagctgctgc acttcgatga tgaccatcag
     1801 caatgtataa agacggtatg ttagcaaata atttggtaat acgatgaata tcgtttgtgt
     1861 cattgattac ccaaaaatgg tgtccgaaac catcaggagc aacaaaatca tatgttgctt
     1921 tttgcataat agttttacta acaatcgcat ctatttcttt attgtttggg tatgctaaaa
     1981 aaacaggttc aatattggca ttgttaatac gaacgtgttt catgcgatct tcctctttat
     2041 cccttcgagt caattcatgt ttttttattt tttcttgcat atagtcatca actgatgcac
     2101 acacaacgag tccgtattgt gttcttccat t
//
LOCUS       DAOSMY010000273         2129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_51948_length_2129_cov_2.783510, whole genome shotgun sequence.
ACCESSION   DAOSMY010000273 DAOSMY010000000
VERSION     DAOSMY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51948_length_2129_cov_2.783510"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLD76_06070"
     CDS             <1..447
                     /locus_tag="PLD76_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU27602.1"
                     /translation="TIVWYDVPTGGQALNDTDLLQDGNTYYGAVRSTSGCESIIRLEV
                     IVDLNKCDDVLIPDGFSPNGDKINDEFVIKNIENKYPNYRLEIYNRYGAILYKGNRNT
                     PNWDGTTTQGGIKFGDSKVPVGVYFYILEFNDGTTKPKQGRLYLSR"
     gene            460..1410
                     /locus_tag="PLD76_06075"
     CDS             460..1410
                     /locus_tag="PLD76_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU27603.1"
                     /translation="MKKRNFRYYFVLIGIFLVSTLVKAQQDPQYTQYMYNMNVINPAY
                     ATDNSGVINLGGLYRTQWVGAVGAPKTATLFAHSPIAKRVEMGVSFINDAIGDVVNEN
                     NIYVDFAYVLPVSEKNKLSFGIKGGATFFNTNFNGFQFTDPDFDFAFENNINEIFPNV
                     GVGTFFFGENYYLGLSAPNLLTTKHINSKNGIKTTGVEEIHYFFTGGYVFDLNSNLKF
                     KPAFMTKAVSGAPLSVDVTTNFLINNKLELGAGYRFDDSVSGLVNFYVLPNLRVGYSY
                     DYTLSNLGRYNSGTHEIFLLFDLDTSSLSSKGFEKSPRFF"
     gene            1422..>2129
                     /locus_tag="PLD76_06080"
     CDS             1422..>2129
                     /locus_tag="PLD76_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27604.1"
                     /translation="MKKYFYILFIFCFSFTLFAQKATEKKANALFEKRSYVKAAEMYE
                     QLKESQVILQNLGDCYYNNSQMIDAVRVYGKLFLSYKDSVAPEYYFKYAHSLLGTADY
                     AKADAIMAEYLKYEISTPKFIANLVTNVPYNYEIQSMSKNTTNGDFGMSFYGDKVAFA
                     SLRNSESKSFGWNDKPYLDLFSANVSDKGLLANVEPFPKEINTKTHESSATFSADGRI
                     MYFNRTNDKKVKIGDEKI"
BASE COUNT          758 a          295 c          387 g          689 t
ORIGIN      
        1 actatcgtgt ggtatgatgt tcctactggc ggacaagcac taaatgatac agatttattg
       61 caagacggaa atacctatta cggggctgta cgttcaactt ctggctgtga gagtataatt
      121 cgcttagaag taattgttga tttgaataaa tgtgatgatg ttttaatccc tgatggtttt
      181 tcaccaaatg gcgataaaat aaacgacgaa tttgttataa aaaatataga gaataaatat
      241 ccaaattaca gattagaaat ttataataga tatggagcta ttctttacaa aggaaataga
      301 aataccccta attgggacgg aactaccaca caaggaggaa ttaaatttgg agattctaaa
      361 gttccagttg gagtatattt ctatatttta gaatttaatg atggcacaac aaaaccaaaa
      421 caaggccgac tttatttaag tagataaatg aaatacaaga tgaaaaaaag aaattttaga
      481 tattattttg ttctcatagg catttttcta gtttctactc tagtaaaagc gcaacaagat
      541 ccacaatata cgcagtacat gtataatatg aacgtgataa acccagctta tgcaacggac
      601 aattcaggtg ttattaactt gggcggattg tatagaacac aatgggttgg agccgtcggt
      661 gcaccaaaaa cagcaacctt gtttgctcat tcgcccattg ctaaaagagt cgagatggga
      721 gtatccttta taaatgacgc tattggagat gttgtaaatg aaaataatat ctacgtagat
      781 tttgcctatg tattacctgt ttctgaaaag aataaactat catttgggat aaaaggaggg
      841 gcaacatttt tcaatactaa ttttaatgga tttcaattta ctgaccctga tttcgatttt
      901 gcttttgaaa ataatataaa tgagattttc ccaaatgtag gtgttggaac atttttcttt
      961 ggagaaaatt attatttagg attgtcagca ccaaatttat taaccacgaa acacatcaat
     1021 agtaaaaacg gaattaaaac tactggagtt gaagaaatcc attatttttt cacaggaggt
     1081 tatgtattcg atttgaactc aaatttgaaa ttcaaaccag catttatgac taaagcagtt
     1141 tctggagctc ctttatctgt agatgtgaca actaattttt tgataaataa taaattagaa
     1201 ttaggtgcag gctatcgatt tgatgattcg gttagcggat tagtaaattt ctatgtcttg
     1261 ccaaatttgc gtgtagggta ttcttatgac tatactttat ccaatttagg aaggtacaat
     1321 tcaggaacac atgaaatatt tttactattt gatttagata cgtctagttt atcatcaaaa
     1381 gggtttgaaa aatcaccaag gttcttctaa tagcgaaaaa aatgaaaaaa tacttttata
     1441 tactttttat attttgtttt agtttcacct tatttgccca aaaagcaact gagaaaaagg
     1501 caaatgcatt atttgaaaaa agatcttacg ttaaagctgc tgagatgtat gagcagttaa
     1561 aggaaagtca ggtgatattg caaaatttag gagattgtta ttataataat tctcaaatga
     1621 ttgatgctgt tcgtgtatat gggaaattat ttttatccta caaggatagt gtagccccag
     1681 agtattattt taaatacgca cattctttac taggaactgc agattatgca aaggcagatg
     1741 ctataatggc agagtatttg aaatatgaaa taagtactcc taagtttatt gctaatctcg
     1801 ttacaaatgt tccctacaat tatgaaatac aatcgatgtc aaaaaataca acaaatgggg
     1861 actttggcat gtcgttttat ggagataaag tagcatttgc ttcattgaga aattcagaat
     1921 ccaagtcatt tggatggaat gacaaaccct atttagattt atttagcgct aatgttagtg
     1981 ataaagggct attggcaaat gtcgaacctt ttccaaaaga aataaatacc aaaacacatg
     2041 aaagttcagc tacttttagt gccgatggac gtatcatgta ttttaatcga acaaatgata
     2101 aaaaggtaaa gataggcgat gaaaaaatt
//
LOCUS       DAOSMY010000274         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_52361_length_2116_cov_3.616691, whole genome shotgun sequence.
ACCESSION   DAOSMY010000274 DAOSMY010000000
VERSION     DAOSMY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52361_length_2116_cov_3.616691"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..803)
                     /locus_tag="PLD76_06085"
     CDS             complement(237..803)
                     /locus_tag="PLD76_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOU27605.1"
                     /translation="MNFSDPHILLFEIQKGNHLAFEFLFKAYYPRLCNFAARFVDSTT
                     AEDIVQECFLKFWEKRFAIKQGNILSLLFTMVRNACLNHLKHKALVDISSIDFVKNLK
                     GEEALYALDFTAGADYVCLEHELAEQINKVMNDLPARCREVFVLSRMDGLKNKEIALQ
                     LNISTTMVEKHIAKAIAVFSAKFKDDIG"
     gene            1175..>2116
                     /locus_tag="PLD76_06090"
     CDS             1175..>2116
                     /locus_tag="PLD76_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOU27606.1"
                     /translation="MVFDEKMIEEVYKNLSSKLETVQQTLQRPLTLTEKILYTHLSTE
                     EQLRLYERAVDYVNFSPDRVAMQDATAQMALLQLMNSGRKRVAVPSTVHCDHLILAHI
                     AAHVDLTTANESNKEVYDFLKAVSNKFGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTPNAGGLGMIAIGVGGADAVDVMAGMPWELKMPKIIGVKLTGRMSGWTSPKDVILK
                     LAGILTVKGGTNAIIEYFGEGTETISATGKGTICNMGAEVGATTSIFPFDEKMATYLQ
                     ATGRESVAALATKYKKHLQADTEVMANPENYYDRVIEI"
BASE COUNT          731 a          396 c          333 g          656 t
ORIGIN      
        1 gccaggaaaa gataaaattg ctttttctta tcatcgttgt cattgatgaa agcaaacaat
       61 tgacgttctt cttctaaact tatttttcct tcaaaatacg cttcagctaa actcttgagg
      121 ttgtcatgtg tactcataat tatactatct tgtatatata tagcaatcaa tgtcgtattt
      181 tacccaacct aaacaacgtt aaacaacgtt aaaacggcac tagtatctgc cattagctag
      241 ccaatatcat ctttaaactt tgcagaaaaa acggctattg ccttggcaat atgtttttca
      301 accattgttg tagaaatatt taattgaaga gctatctctt tattttttaa tccatccatt
      361 ctgcttaaaa caaacacctc tctacaccga gcaggtaaat cattcattac cttattaatc
      421 tgctctgcta gttcgtgttc caaacaaaca taatctgctc ctgccgtaaa atccaatgca
      481 tacaaagcct cttctccctt caaatttttc acaaaatcta ttgaagatat atcaactaga
      541 gctttatgtt ttaagtgatt taggcatgca ttgcgaacca tcgtaaataa taaagagagg
      601 atattgcctt gttttatagc aaatctcttt tcccaaaatt tcagaaagca ctcttgaaca
      661 atatcttctg cagtagtaga atctacaaat cgagctgcaa aattacacaa acgaggataa
      721 tatgccttga ataaaaattc aaaagccaaa tgattgcctt tttgaatttc gaacaacaat
      781 atgtgtggat ctgaaaaatt catagtgtga tttagatttt acaaaggtaa caattttaca
      841 aaataaatga ttctttcaag ctattttttt cgcataacaa tggacttcaa cgaaaactat
      901 gttctcctat actcattcta ttcatatttt tataccataa tattgattat aaaattgagc
      961 tctcattaaa caataattaa tcgaaacaag tatctattat ttgttatcac atgactctca
     1021 ttttgtatat attccgcata tatacaaaat aaaacactaa aaactctaaa ttgctacatt
     1081 ttttcataca aaaaagcact tctttctatt ttctcatagt ttttttagta tatttgcaaa
     1141 aacgaaaaat ttaactacaa actgaaagaa taatatggtt tttgatgaga aaatgattga
     1201 agaagtatat aaaaacttgt catcaaagct tgaaacagta caacaaacac tacaacgtcc
     1261 tctcacctta acagaaaaaa ttttatatac tcacctctca accgaggaac aattacgact
     1321 atatgaaaga gccgtggatt atgtaaattt ctctcccgac cgtgtagcca tgcaagatgc
     1381 tactgcacaa atggcattgt tacaattaat gaattccgga cgcaaaagag tagccgttcc
     1441 gtctacagta cattgcgacc acttaatact cgctcacatt gctgctcatg tagatttaac
     1501 tacagccaat gagtcgaaca aagaagtata tgatttttta aaagctgtat ccaacaaatt
     1561 tggtattggc ttttggaaac ctggtgcagg cataatacac caagtggtat tagaaaacta
     1621 tgcgtttcca ggaggaatga tgataggaac agactcgcat acacccaatg caggtggttt
     1681 gggaatgatt gccattggcg tgggaggagc tgatgctgtt gacgtaatgg caggtatgcc
     1741 ttgggaactg aaaatgccta aaatcattgg agtgaaatta actggaagaa tgtctggctg
     1801 gacttcaccc aaagatgtaa tccttaaact tgctggtata cttactgtaa aaggaggaac
     1861 caatgccatc attgaatatt ttggcgaagg cacagaaacc atctctgcta cgggcaaagg
     1921 cacaatttgt aatatgggag cagaagtagg tgccacaacc tccattttcc cttttgatga
     1981 aaaaatggct acatacttac aagccacagg cagggaatct gttgctgcat tagctaccaa
     2041 atacaaaaag catttgcaag ctgatacaga agttatggct aatccagaaa attattacga
     2101 tagagtcatt gaaatt
//
LOCUS       DAOSMY010000275         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_52376_length_2116_cov_2.784571, whole genome shotgun sequence.
ACCESSION   DAOSMY010000275 DAOSMY010000000
VERSION     DAOSMY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52376_length_2116_cov_2.784571"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PLD76_06095"
     CDS             complement(<1..1770)
                     /locus_tag="PLD76_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27607.1"
                     /translation="MNTSRTSTSFLYPLLSWNFVLRLLFIGLFLLVSLANTFAIKVRN
                     DNAWASVRIHYWNGGTGSDWGSRPTMTAATTPAWDTSGKSWVEWNINTCTDIKLTGTN
                     EPPSTNDINGVSNDGCYTMSSDATPTLTRVDCATGGAYVPPVPVINFAVTGGVVSGTT
                     EWNTSPPSSCIFEETGTNTYSLCFIYDSKAFKLLRKENGLPNTNWDWSSAVAIVAGSN
                     VSGSSGDLTITGFTTGDPVCLQLVVSGTTYTLSAEQSVYITSDLPNPINQGADITLTA
                     NNVSSACTWEYSTDGGTTWQPYTGATSGTYNEEISPSPLTNMTYRVTSDGKTATYMVQ
                     VVINCQGSIQTHFTETFGTLPTATARTTSAHIHSSVYQYSPEGKEVHDGWYAVVANPR
                     NCGRGTRQSTIVDGVVTVLGDVRTSGDYWYNDRTDHTGDTHGGMLFVNCKDKGEIIYQ
                     RTITDLCANIYMNFSAWFANAAVPSSTAPINTQFRILDQNGDEIQSARINVNNIKASD
                     GWVHGSTAFFSGNNTQLTLQIINNGEAGVGNDILIDDISFTSCVPKLSIQAGLEVECG
                     LVTELSVEHDGINTVFNGPPFYLW"
     gene            complement(1885..>2116)
                     /locus_tag="PLD76_06100"
     CDS             complement(1885..>2116)
                     /locus_tag="PLD76_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27608.1"
                     /translation="DMNYVPSSYTYITITQHYTDNFKQVGKGFGNTFHENFYRYRIDY
                     TFTNKQINPLKIKTHPYDISDHFPLQLDFQTP"
BASE COUNT          639 a          409 c          420 g          648 t
ORIGIN      
        1 ccacaggtag aatgggggac cattaaatac ggtatttatg ccatcatgct caacagataa
       61 ttccgtaacc aaaccacatt caacctccaa tcctgcttga atagaaagtt ttgggacaca
      121 agatgtaaag gaaatatcat caattaaaat gtcattacct actccagctt ctccattgtt
      181 gattatctgt aaggtaagtt gtgtattgtt gcctgagaaa aaagccgtag acccatgtac
      241 ccaaccatca ctggctttga tattatttac attgatacga gccgattgta tttcatctcc
      301 attctggtcg agaatacgga attgtgtatt gataggtgcc gtggacgacg gaacagctgc
      361 attggcaaac caagcagaga aattcatata aatattggca cataagtcag taatggttct
      421 ttgataaata atctcacctt tgtctttgca gttcacaaat agcataccac catgtgtgtc
      481 ccctgtatgg tcggttctgt cgttgtacca ataatcacca ctggtacgta catctcctaa
      541 tacagtaact acgccatcta caatagttga ttgtcttgtt cctctaccgc aattacgagg
      601 gttagccact acggcatacc aaccatcgtg tacttctttt ccctcagggc tatattgata
      661 aactgatgaa tgtatatgag cagaagttgt tcgtgcagta gctgtaggca aagtgccaaa
      721 agtttctgta aaatgagttt gaatacttcc ttggcagtta ataacaactt gtaccatata
      781 cgtagcagtc ttcccatcag atgtaactct atacgtcata ttggtaagag ggcttggaga
      841 tatttcttca ttgtatgtgc cactggttgc tccagtataa ggttgccatg ttgtaccacc
      901 atcggttgaa tattcccatg tacaagcaga agaaacatta ttcgccgtta atgtaatatc
      961 tgctccttgg tttattggat taggaaggtc agacgtaata tatacacttt gctctgctga
     1021 taatgtgtag gtagttcccg atacgactag ttgtaagcag acaggatctc ccgtagtaaa
     1081 cccggtaata gttaaatctc cagaactacc actcacatta cttcctgcta caatcgcaac
     1141 tgcacttgac caatcccaat tcgtatttgg cagcccgttt tcttttcgta acagtttaaa
     1201 tgctttggag tcgtaaataa agcaaagaga ataggtgttg gttcctgttt cttcgaaaat
     1261 acatgatgaa ggaggagatg tgttccattc ggtagtgcca cttactacac caccagttac
     1321 tgcaaaattt attacaggta caggcggtac atacgctcca ccagtagcac aatcaactcg
     1381 tgttaatgta ggggtagcat cagaactcat ggtgtagcaa ccatcattac ttacgccatt
     1441 gatgtcattt gtactgggag gttcatttgt tccagttagt ttgatgtctg tacacgtatt
     1501 aatattccac tccacccagc ttttaccact tgtatcccaa gcaggtgtag tggcagctgt
     1561 catcgttggg cgagaacccc aatcagaacc agttcctccg ttccaataat gtatgcggac
     1621 ggaagcccac gcgttatcgt ttctaacttt tattgcaaag gtatttgcaa gactgactag
     1681 taaaaatagt ccaataaata ataggcgtaa tacaaaattc cacgagagga gtgggtacaa
     1741 aaaggacgtg ctagtacgtg atgtattcat actatgttga gttaaggtta gcgtaacagt
     1801 atatgcccca aagttaaaaa aaaataaatg aaatacaaaa tttcttgaaa gaaaaagttt
     1861 atcgtttcaa tagtagggag ttaactaagg tgtctgaaag tctagttgta aggggaaatg
     1921 gtcagaaata tcataaggat gagtttttat ttttaatggg ttgatttgct tatttgtaaa
     1981 agtataatca atacgatatc ggtaaaagtt ttcgtgaaac gtattgccaa aacctttgcc
     2041 tacttgttta aaattgtctg tataatgttg agtaatcgtt atatatgtat acgaagaagg
     2101 gacataattc atatct
//
LOCUS       DAOSMY010000276         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_52409_length_2115_cov_2.995631, whole genome shotgun sequence.
ACCESSION   DAOSMY010000276 DAOSMY010000000
VERSION     DAOSMY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52409_length_2115_cov_2.995631"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLD76_06105"
     CDS             complement(<1..902)
                     /locus_tag="PLD76_06105"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOU27609.1"
                     /translation="MKKLHKILIMSMLLISLSNCVTKIPEPPKDIDSDFVEDHTFDTP
                     VVITFNENSVTANPASVSGITFTINEGHIVVNSEHTNVEYILTGNTKNGSFKLYSTSK
                     CKLTLKNVHIESDNSPAINIQTSKSIFVNIANGTENTLSDKNPYILTELEDAKGTFFS
                     EGQLIFSGTGSLQIASKQAHGIITDDYIRIRSGEIRINEAVKDGFSAKDHILIEGGDI
                     YLIVSDDGISCRQGYITIEDGNITVESMDEGIVAGYDPETDLVIEGVVPDITINGGAI
                     TIKTDGARGHGIKSVGMITFNGGNT"
     gene            complement(1102..1884)
                     /locus_tag="PLD76_06110"
                     /pseudo
     CDS             complement(1102..1884)
                     /locus_tag="PLD76_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT          621 a          411 c          318 g          765 t
ORIGIN      
        1 gtatttcctc cgttaaaagt aatcattcct accgacttta ttccgtgtcc tcttgctcca
       61 tctgttttta tcgtaatagc acccccattt atggtaatgt ctggaactac tccttctatt
      121 accaagtctg tttcaggatc ataccctgca acaatgcctt catccattga ttcaactgtt
      181 atattgccat cttcaatcgt aatgtagcct tgtctgcacg aaataccatc atcagacacg
      241 ataagataaa tatctcctcc ttcaattaaa atatgatcct tagcactgaa tccatctttt
      301 actgcttcat taatacgtat ttcaccgctt cgtatacgta tgtaatcatc agttataatg
      361 ccatgtgctt gtttactagc tatttgtaaa gaacctgttc cgctaaaaat aagttgacct
      421 tcgctaaaaa atgttccttt tgcatcttct aattccgtta gtatatatgg atttttatca
      481 gacaatgtgt tttcagtacc attcgcaata tttacaaata tacttttgct ggtttgtatg
      541 ttgatggcag gcgagttatc actttctatg tgcacgtttt ttaatgttag tttacatttt
      601 gatgtgctgt ataatttaaa tgatccgttc ttcgtattac ctgttagtat atactctaca
      661 tttgtgtgtt cagaattcac aacaatatgt ccctcattaa tagtaaaggt aataccacta
      721 acagaagctg ggttggctgt aacagaattt tcattaaatg taatgacaac aggagtgtca
      781 aaagtatgat cttcaacaaa atcagaatca atgtcttttg gtggctcagg aattttggta
      841 acgcaattac taagcgagat aagtagcata ctcattataa gtattttgtg taattttttc
      901 atacaattat ttaaggttaa caaaatacaa aaatacacct ttttagctaa tattctcatt
      961 tttatacaaa aaaaatagaa tttaacgtaa aaaaaacact attttcgttt ttcaacaaaa
     1021 atagtgttct atctaaaata gtctcttttc agtttattcg ttggctgcta aaccttttct
     1081 gtaatcttca attacggttt cactcatact catgctcgaa aaacctccat cgtgaagtag
     1141 attctgcata gttacttttt tagttaagtc gctaaacatt actacgcaat aatcagcaca
     1201 atctgcagct gttgcgttgc caagaggaga taatccttct gcaaaatcaa gcaaggtgtc
     1261 taatcctttt acgccattcc cagcggtagt tggtgttggt gattgagaga tagtattgat
     1321 acgtactttt ttatcacgac catatatata accaaaactt cgtgcaatgg attcgagcaa
     1381 tgcttttgca tcagccatgt cgttgtatcc acatacagtg cgttgagcag ccatatacga
     1441 taaagctatt acagaaccat attcattgat agcatccatc ttttttgcta cttgcaatac
     1501 tttgtgaaaa gaaatagcag atatatcaag ggtagtacta agcattccat agtctaaatc
     1561 atcgtaagca cgacctttgc gaacatttgg tgacatacca attgaatgca agacaaaatc
     1621 aattttacca cctaagattt ttattgattc agcaaataca ttttccaaat cggctacgtt
     1681 tgtagcatcg gctgggataa tttgagcatt taatttcttg cctaattcgt tagttgtacc
     1741 catacgtaca gctaaaggtg tgttggtgag agtaatggta gcaccttctt ctactactct
     1801 ttcagctact ttccatgcaa tagacatatc gtttaatgca ccgaagataa ttcctctttt
     1861 tccttttagt aagttatttc ccatgttttc tgtttattaa aattgcacaa aattactcct
     1921 tttgttcaat aagttccaaa ctaaatccct ctttttttat tttttctatc aatctcatat
     1981 tttttcaaca tgctttttgt ttgtaataag gtctttgccc tcccaaatta cataagcctt
     2041 atgtgcttga tttaaaaaca tttgccatcc gtcacaggtc gttgctcctc gtgtttcggc
     2101 ttggtgtaga aaaag
//
LOCUS       DAOSMY010000277         2115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_52411_length_2115_cov_2.906796, whole genome shotgun sequence.
ACCESSION   DAOSMY010000277 DAOSMY010000000
VERSION     DAOSMY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52411_length_2115_cov_2.906796"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLD76_06115"
     CDS             <1..755
                     /locus_tag="PLD76_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU27610.1"
                     /translation="DVAYINEVINKTKNTYTVNNEKVFMLGASMGGAMAYKYAFSANP
                     QITAMAIISGFKGAEVSTSGDFPVATCHFHSTNDEVVEYNGTAFNTPIQELVTLLSGK
                     NHTTTAEEIAIPNAEEEDNMTVTAYDYQTASKPRFLFYKIEGANHMLTGLKDITIFTE
                     ALKFFNNEPILTSITDTYVAQVNVSPNPTSDYIYVSVSGNYTLYTLQGNIILAGSAHE
                     NEAIAVGHLPKGLYLLSIENNGTISSAKVAIK"
     gene            760..942
                     /locus_tag="PLD76_06120"
     CDS             760..942
                     /locus_tag="PLD76_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27611.1"
                     /translation="MDIQLIIVIIASILALLWVVWRVYRTFKQSDSAKNVCDSCAHSA
                     SCKFKEECNDILHKQS"
     gene            955..1794
                     /locus_tag="PLD76_06125"
     CDS             955..1794
                     /locus_tag="PLD76_06125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU27612.1"
                     /translation="MKSLFLIKTILILALTQFSFMTNAQITNSHTLVKISTEYGDMTV
                     KLYNETPLHRDNFIKLVEQGFYNDLLFHRVINGFMIQGGDPQSKNAPKGKQLGSGDVG
                     YTIPAEFVYPQYYHKKGALAAARQGDQMNPKKASSGCQFYIVQGNKIGDAELQQTEQQ
                     AKHKAKQTLFYALADQHKENIKQLRINKDTAGLEKLQTELIAQVEKELATQSFTLPET
                     LKNDYKTLGGTPFLDNEYTVFGEVIDNLELIDKIAATKTLSGDRPEKDIKMTLTILQK
                     GNY"
BASE COUNT          764 a          464 c          358 g          529 t
ORIGIN      
        1 acgatgtagc ctacatcaac gaggtaatca ataaaacaaa aaatacctac accgtaaaca
       61 acgaaaaagt atttatgcta ggagcatcta tgggtggtgc tatggcatac aaatatgctt
      121 ttagtgctaa tccgcaaatt acggctatgg ctatcattag cggttttaag ggtgccgagg
      181 taagcacatc aggggatttc cccgttgcca cctgccattt tcatagcacc aacgatgaag
      241 tggtggaata caacggaact gcatttaata cacctatcca agaattggtt accttattgt
      301 cgggcaaaaa ccatacaact actgccgaag aaattgctat acccaacgcc gaagaagaag
      361 ataatatgac ggtaactgcc tacgactacc aaacagcaag caaacctcgc tttttgtttt
      421 acaaaataga aggtgcaaac cacatgctaa cgggcttaaa agatattacc atttttacag
      481 aagctttgaa atttttcaac aacgaaccaa ttcttacttc tattaccgac acatacgtag
      541 ctcaagtaaa cgtttctcct aaccctacta gcgattatat ctatgtatct gttagtggca
      601 actatactct gtacacatta caaggcaata tcatactcgc tggtagtgct cacgaaaacg
      661 aagccattgc tgtgggtcat ttacccaaag gcttgtatct actatctatt gaaaacaacg
      721 gcacaatcag ctctgccaaa gtagctatta aataagtaaa tggacataca acttatcata
      781 gtcattatag cctccatact cgccctattg tgggtagtat ggagggtgta tcgcacgttt
      841 aaacagtccg attcagcaaa aaatgtctgc gatagttgtg cccatagtgc tagttgcaag
      901 ttcaaagaag aatgcaacga tattctacac aaacaatctt agtttttcat ttttatgaaa
      961 tcgctttttt taatcaaaac cattttaata ctcgcactta cccaattctc ttttatgaca
     1021 aacgcacaaa taaccaactc gcacaccctc gtaaaaatta gtaccgaata cggcgatatg
     1081 accgtaaaat tatataacga aactccccta caccgcgata attttatcaa attagttgaa
     1141 caaggttttt acaacgacct attatttcat cgcgttatca acggatttat gattcaagga
     1201 ggcgaccctc aatcgaaaaa cgcacccaaa ggcaaacagc ttggtagtgg cgatgtgggt
     1261 tataccatac ctgccgaatt tgtgtatcca cagtattacc acaaaaaagg agcattggca
     1321 gccgctcgtc aaggcgacca aatgaatcct aaaaaagcct catcgggttg tcaattttat
     1381 attgtacaag gcaacaaaat aggcgatgcc gaactacaac aaacagaaca acaagcgaaa
     1441 cacaaagcca aacaaacact gttttatgcc cttgccgacc aacacaaaga aaatatcaaa
     1501 cagctacgta taaacaaaga tactgctggt ttagaaaaac tacaaacaga attaattgct
     1561 caagtagaaa aagaactcgc tactcaatca ttcaccctgc ccgaaacact caaaaacgac
     1621 tacaaaacgc tgggtggtac accctttttg gacaatgaat acaccgtttt tggcgaagta
     1681 atcgacaacc tagagcttat tgataaaata gcagctacca aaaccctatc aggcgaccga
     1741 ccagaaaagg acatcaaaat gacactaacc attctacaaa aagggaatta ctaaagaatc
     1801 aagcagtcaa ctatttgcag catttttaca ctaaaaattt ttgcaaaaaa gtgggttatc
     1861 tcaaaataaa ccattacctt tgcggctcat ttttaatcaa cataatgtct aacaactaat
     1921 taggaaacaa aaaaacatga caaaagaatt tattcaacct gatgagagtt tcgactgggg
     1981 agcatacgaa caaggtgcta cctacacaga aattagcaaa gaagacctcc aaaaggctta
     2041 caatcaaaca ctcaaccaag taaatgccaa cgaagtagtg gatggtactg ttatttcgat
     2101 gaacaaacgc gaagt
//
LOCUS       DAOSMY010000278         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_53069_length_2095_cov_3.407353, whole genome shotgun sequence.
ACCESSION   DAOSMY010000278 DAOSMY010000000
VERSION     DAOSMY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53069_length_2095_cov_3.407353"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /gene="murD"
                     /locus_tag="PLD76_06130"
     CDS             <1..1273
                     /gene="murD"
                     /locus_tag="PLD76_06130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU27613.1"
                     /translation="KQGFDVFVSDASGIKQKYREVLQKYAIRWEEGTHTYSEIMDATE
                     VIKSPGIPDKAPLIKEIKARHIPIISEIEFAGRYTKAKTICITGSNGKTTTTMLTYHI
                     LQQAGLNVGLAGNVGKSFALQVAENQFDYYVIELSSFQLDNMYTFRADVAVLLNITPD
                     HLDRYDYNFQNYVNAKMRITQNQTENDVFIYWQDDPSINQELTKHTIKAKLYPFSMKK
                     KDNTKSFVDKNNLIINTEETDMSINIDNLAIKGTHNVYNSMAAALSASVMNIKKEQIR
                     NSLADFEGVEHRLEPVAKVRNVMFINDSKATNVNSCWYALESMKTPVVLILGGTDKGN
                     DYNEIKSLVEQKVHTLVFLGIDNSKLEAFFGKLNKKIISTSSMDDAVKAAYLAANKGD
                     SVLLSPCCASFDLFTNYEDRGTQFKNCVKNL"
     gene            1295..>2095
                     /locus_tag="PLD76_06135"
     CDS             1295..>2095
                     /locus_tag="PLD76_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU27614.1"
                     /translation="MGELFKKVFKGDFILWITIGLLLLVSSIEMFSAISQEAFRRSNH
                     LAPFFSHIQMLGLGLIAMIIVHRFPYRYIRGLNYLILPIALVLLVLLPFIGVEANNAI
                     RAIKVLGFEFQPLELAKFSTIVFISDMLARNQKEGQTADKAFWPIISVLGIFCLLIAP
                     QNLSTAVMLFGVGILLMMIGRISFAKLGITIGVLAALLLSLYVISHYFQPQFLGRLNT
                     WVGRIDDFVSEINEPSADKTYVITDDNMQIMHSKIAIAHGMTPAGPGNS"
BASE COUNT          739 a          356 c          378 g          622 t
ORIGIN      
        1 aaaacaaggc tttgatgtat ttgtttcaga cgcttctggt atcaaacaaa agtatcgaga
       61 agtattacaa aaatatgcta ttagatggga agaaggaaca catacatact cagaaataat
      121 ggatgctaca gaggtgatta aaagccctgg cataccagac aaagcccctc ttattaaaga
      181 aataaaagct agacacatac ccattatttc ggagatagag tttgctggtc gttatacaaa
      241 agctaaaacc atttgtatca caggaagcaa tggaaaaacc accaccacaa tgctaaccta
      301 tcacattttg caacaagcag gattgaatgt gggactagca ggcaacgtgg gtaaaagctt
      361 tgcactacaa gtagcagaaa atcagtttga ttattatgta attgaactaa gcagttttca
      421 actcgacaat atgtatactt tcagagccga cgtagctgta ttgttaaaca ttacacccga
      481 tcatttggac aggtatgact acaattttca aaactatgtg aatgctaaaa tgcgtattac
      541 acaaaatcaa acagaaaacg atgtatttat ctattggcaa gatgatccat ctattaacca
      601 agaactaacg aaacatacta taaaagcaaa actatatcca ttttctatga aaaagaaaga
      661 taacaccaaa tcatttgttg acaaaaataa ccttataatc aatacagaag aaaccgatat
      721 gagcatcaac atcgacaact tggcaatcaa aggcactcac aatgtgtaca attcaatggc
      781 agcggcacta tctgctagcg taatgaacat taaaaaagag cagataagaa actctttggc
      841 agattttgaa ggagttgaac atcgtctaga acctgtagca aaggtacgaa atgtcatgtt
      901 tattaacgat tcaaaagcca ccaatgttaa ctcgtgttgg tacgcacttg aaagtatgaa
      961 aacaccagtg gtattaattt taggtggtac agacaaaggc aatgactata acgaaataaa
     1021 aagcttggta gagcaaaaag tacacaccct ggttttttta ggtattgaca atagtaaatt
     1081 ggaagcgttt tttgggaaac taaataaaaa aataattagc accagttcga tggatgatgc
     1141 tgtaaaagct gcttatttag cagccaacaa aggagattct gttttattat caccctgctg
     1201 tgccagtttt gatttattta ctaactacga agatagagga acacaattta aaaactgtgt
     1261 taaaaattta taacaatttg atgtaagaat aattatgggc gaactattta aaaaagtatt
     1321 taaaggagat tttatcttat ggattaccat aggacttctc cttttggttt cgtctatcga
     1381 aatgtttagt gctatcagtc aagaggcttt tagacgcagt aatcatcttg ctccattttt
     1441 tagccatatt caaatgctcg gattaggctt aatagccatg attattgtac atcgttttcc
     1501 gtatcgctat atcagaggac taaactattt gatactcccc attgcattag ttttgctagt
     1561 attattacca tttataggag tagaagcaaa taatgcaata cgtgcaatta aagtattagg
     1621 atttgagttt cagccactag aattagcaaa attcagtacc attgtattta tcagtgatat
     1681 gcttgcaaga aatcaaaaag aaggtcaaac tgccgataaa gctttttggc ccatcatttc
     1741 agttttaggc atattctgtt tgttaattgc cccacaaaac ctttctacag ccgtaatgtt
     1801 attcggagta ggtattctat taatgatgat aggtagaatt tcctttgcca aattaggtat
     1861 cactattgga gtgttggcag ccttattact ttctctttat gttatttcac attactttca
     1921 gccacaattc ttaggtcgtt taaatacgtg ggtaggtcgt atagacgatt ttgttagcga
     1981 aataaatgaa ccttctgcgg acaagacata tgttatcaca gatgataaca tgcaaatcat
     2041 gcactctaaa attgcaattg cacacgggat gacacctgca ggtccgggaa atagt
//
LOCUS       DAOSMY010000279         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_53215_length_2091_cov_3.594303, whole genome shotgun sequence.
ACCESSION   DAOSMY010000279 DAOSMY010000000
VERSION     DAOSMY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53215_length_2091_cov_3.594303"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLD76_06140"
     CDS             complement(<1..895)
                     /locus_tag="PLD76_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU27615.1"
                     /translation="MEKEYLVYKLSETIKTTTRIDRELFTSYNVKRGLRNEDHSGVLV
                     GLTNIGDVVGYERLPEGGLKAIPGKLFYRGIDVEDLAKGIQKDNRVGFEETAFLLLSG
                     YLPDKEELDTFSSILNDSMVLEKKMKMNILELEGQNIMNILARSVLEMYTFDENPDDI
                     SRDNLIRQSIDLIAKFPTIIAYAYNMMRHSTHCRSLHIRHPKDKVSLAENFLYMMQGP
                     RSYSDLDVKILDLALILHAEHGGGNNSTFTVRVTSSSETDTYSSIAAGIGSLKGPLHG
                     GANLQVVDMLEHLKEAISDWTD"
     gene            complement(910..>2091)
                     /locus_tag="PLD76_06145"
     CDS             complement(910..>2091)
                     /locus_tag="PLD76_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOU27616.1"
                     /translation="TNSSYEDLARAASVVAKAKETNIPVKAKLIINPGSELVRYTAER
                     DGLLETFESVGGVIMANACGPCIGQWKRESTTPDRPNSIVTSFNRNFAKRNDGNPNTH
                     SFVASPEIVAALTIAGDLCFNPLKDKLSTKNGELVKLPEPFGDELPKKGFEVKDAGYI
                     APSENPDQAVVEIDPQSKRLQVLEAFSAWDGKDIIKQPLLIKVKGKCTTDHISMAGPW
                     LRYRGHLDNISDNMLIGAVNAFTDKTNAVLDTETGDYIAVPALARKYKTAGIGSIVVA
                     EDNYGEGSSREHAAMEPRFLNVKAIVVKSFARIHETNLKKQGLLALTFVNKDDYSKIR
                     ENDTISILGVTNMKPGVPLTMVLHHVDGTEESFDVAHTYNLAQINWYKSGSALNALNK
                     I"
BASE COUNT          575 a          439 c          374 g          703 t
ORIGIN      
        1 catctgtcca atcggaaata gcttctttta ggtgctccaa catatctact acttgcaagt
       61 tagcacctcc atgaagaggt ccttttagtg aaccaatacc tgccgctata gaagagtagg
      121 tgtctgtttc agacgagcta gtaacacgca ctgtaaaggt ggaattatta cccccgccat
      181 gttcggcgtg caaaatgagg gctaagtcaa gaatttttac atccaaatcc gaatagctac
      241 gtggaccttg catcatgtac aaaaagtttt cggctaaaga aaccttgtct ttagggtggc
      301 gaatatgcaa cgatcgacaa tgcgtactat gacgcatcat attgtatgca taggcaataa
      361 tcgttggaaa ttttgctatt aagtcgatag attggcgaat gagattatcc cgagaaatgt
      421 catctggatt ttcatcaaac gtatacattt cgagcacact tcgagccaaa atattcatga
      481 tgttttgccc ttcgagttcc aaaatgttca ttttcatttt tttctcaagc accattgagt
      541 cattcaggat actagaaaaa gtatctaatt cttctttgtc gggtaaatag ccagaaagca
      601 acaagaatgc tgtttcttca aaacctacac ggttgtcttt ttgaattcct tttgctaaat
      661 cctcaacatc tattcctcta taaaacaact ttcctggaat agcttttaat cctccttcgg
      721 gtagtctttc gtagcccacc acatcgccaa tgttcgttaa tccaaccaat acgcccgaat
      781 ggtcttcgtt acgcaaacca cgttttacat tataggaggt aaataattct ctatcaattc
      841 gtgtggtagt tttaattgtt tctgacaatt tatataccaa atattctttt tccattctag
      901 tttgtattat taaattttgt ttaacgcatt tagagcactt cccgatttgt accagtttat
      961 ttgtgctaag ttataggtat gtgctacatc aaatgattct tctgttccat caacatgatg
     1021 taacaccatt gttaagggga ctcctggttt catgttcgtt acacccaaaa tactgattgt
     1081 atcattttcg cgaattttgg aataatcatc tttattaaca aatgtaagag caagtaatcc
     1141 ttgctttttc aagtttgttt cgtgtattcg agcaaaagat ttaaccacaa ttgcttttac
     1201 gttcaagaaa cgtggctcca tagcagcatg ttcgcgactt gagccttctc cataattatc
     1261 ttcagccacc acaattgagc ctattccagc agttttgtat ttgcgagcta acgctggtac
     1321 agcaatgtaa tctcctgttt cggtatcgag tactgcattc gttttgtccg taaaggcatt
     1381 aaccgctccg attaacatat tatccgaaat attgtctaag tgacctctgt aacgcaacca
     1441 aggaccagcc atcgagatgt ggtcggtggt gcatttccct ttcactttga tgagtaaagg
     1501 ttgttttata atgtcttttc catcccaagc tgaaaatgct tctaatactt gtaatcgttt
     1561 tgattggggg tctatttcta caacagcttg atcaggattt tcagagggag ctatatagcc
     1621 tgcatctttt acttcaaacc ctttcttagg aagttcatcg ccaaaaggtt cgggaagttt
     1681 tacaagttct ccattcttag tagaaagttt gtctttcaac ggattaaaac acaaatcacc
     1741 agctattgta agtgccgcta ctatttcggg tgatgcaaca aagctatgtg tattaggatt
     1801 cccatcattt ctttttgcga aatttctgtt gaatgaagtt acaatggaat tgggtctatc
     1861 aggagttgtt gattcacgtt tccactgacc aatacatgga ccacaagcgt ttgccataat
     1921 aacaccacct acactttcga atgtttccaa tagtccatct ctttcggcgg tataacgcac
     1981 caactcagaa cctggattaa tgattaactt tgcttttacc ggaatgtttg tttctttcgc
     2041 ttttgctact accgatgctg ccctagctaa atcctcgtat gacgaattgg t
//
LOCUS       DAOSMY010000280         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_53397_length_2085_cov_3.666010, whole genome shotgun sequence.
ACCESSION   DAOSMY010000280 DAOSMY010000000
VERSION     DAOSMY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53397_length_2085_cov_3.666010"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /gene="recF"
                     /locus_tag="PLD76_06150"
     CDS             complement(<1..1079)
                     /gene="recF"
                     /locus_tag="PLD76_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852665.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOU27617.1"
                     /translation="MVLTKLSIVNFKNIADASLDFCAGINCFIGNNGMGKTNLLDAIY
                     YLSFCKSHTNAIDSQNVKHDEGFLMLQGNYIHQQQEEEIYVGLKRGQKKLFKRNKNAY
                     ERISDHIGLIPLVLVSPSDAVLIDGGSDERRKFIDGVLSQYDKKYLDCLLKYNHVLQQ
                     RNALLKSNAVEETMYEVCEMQMAHFADYIYAQRTQFISQFLPVFQQYYVAIANVNEQV
                     SISYKSHIEDGDLTIQLKQSRAKDTLLGFTTKGIHKDELEFYLGDYLVKRVGSQGQNK
                     TFLVALKLAQFEFLKQVHACNPLLLLDDIFDKLDANRVSKIIQMVSGDNFGQIFITDT
                     NREHLVSILQQLPQENRIFLVNEDG"
     gene            1253..>2085
                     /locus_tag="PLD76_06155"
     CDS             1253..>2085
                     /locus_tag="PLD76_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU27618.1"
                     /translation="MEYTFKEIEQKWQQYWKNNATYKVEINATKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRFKRLKGYNVLHPMGYDAYGLPAEQYAIQTGQHPAITTTK
                     NIARYREQLDKIGFSYDWNREVRTCEPDYYKWTQWAFVKMFNHYYDTQLQQAKPIESL
                     VKHFATQGTDSLQAACTEELSFTAKEWNEKTDEEQQHILLNYRIAYLADLKVNWCPAL
                     GTVLANDEVSEGVSIRGGHPVEQRIMRQWSLRISAYAQRLLDGLDTIDWSESIKETQR
                     N"
BASE COUNT          694 a          392 c          359 g          640 t
ORIGIN      
        1 ataccgtctt catttactaa aaaaatgcga ttttcctgtg gtagctgttg taaaatactc
       61 actaaatgtt ccctattcgt gtctgtaata aaaatttgac caaaattatc tcctgatacc
      121 atttgtatta ttttacttac tctatttgca tctaacttat caaaaatatc atctaataat
      181 aataggggat tacaggcatg cacttgtttc aaaaattcaa actgtgccaa tttgagggct
      241 actaaaaagg ttttgttttg accttgtgag ccaactcttt ttaccaaata atctcctaaa
      301 tagaattcca gttcatcttt atgaattcct tttgtggtaa aacctaagag agtatctttg
      361 gctctgcttt gtttaagttg aatggttaaa tcaccatcct caatatgcga cttgtagctt
      421 atgctaactt gctcatttac attggcaata gctacataat actgctgaaa aactggtaaa
      481 aattgagaaa taaactgggt acgttgagca tatatgtagt ccgcaaaatg tgccatttgc
      541 atttcgcaca cttcgtacat agtttcttct actgcattgg atttaagcaa tgcgttgcgt
      601 tgttgcaaca catgattgta tttgagcaaa caatcaaggt attttttatc gtactgagaa
      661 agaacgccat caataaactt ccgtctttca tcactaccac cgtctatcaa cacagcatca
      721 cttggcgaaa ccaacaccaa tggaattaat ccaatatggt cggatattct ttcataagca
      781 tttttattcc gtttaaataa ctttttctga ccacgtttca accccacata aatttcttct
      841 tcttgttgtt ggtgaatata attcccctgc aacattaaaa agccttcatc atgtttaaca
      901 ttttgactat caattgcatt tgtatgactt ttgcaaaaag aaagataata aatagcatct
      961 aacagattgg ttttccccat tccgttgttt ccgataaagc agttaatacc agcacaaaag
     1021 tcaaggctag catctgcaat atttttaaag tttactatgg ataatttggt aagaaccata
     1081 ctgtaatttt tatgcaaaaa tagcaaaaaa gtttgattga atgcgttaaa aaatagaatc
     1141 aatgtttacg aaggaagtgg tatacatata aaatgataga aaaatatggg aaagcactgt
     1201 attttcatta actttgtgcc ctaaataact gaaaatactc tcacaagcta ttatggaata
     1261 tacatttaaa gaaattgaac aaaagtggca acagtattgg aaaaataatg ccacatacaa
     1321 agtagaaatt aacgctacta aaccaaaata ctatgtattg gatatgtttc cgtatccatc
     1381 gggagcaggg ctacatgttg ggcacccgct ggggtatatt gcatctgaca tattcagtag
     1441 attcaaacgc ctaaaaggat ataacgtatt gcatccgatg ggttatgatg cttatggatt
     1501 gcctgccgaa caatatgcca tacaaacagg tcagcatcct gccattacaa ccactaaaaa
     1561 cattgctcgc taccgcgaac aactagataa gattggattt agttacgatt ggaatcgtga
     1621 agtacgcact tgcgagcctg attattacaa atggacacag tgggcttttg taaaaatgtt
     1681 taatcactat tacgacacgc aattacaaca agccaagccc atagaaagtt tggtaaaaca
     1741 ctttgcaacg caaggcacag attcattgca agccgcttgt actgaagaac ttagttttac
     1801 agccaaagaa tggaacgaaa aaactgacga agaacaacaa cacatattgt tgaattatcg
     1861 cattgcctat ttagctgact taaaagtaaa ctggtgtcct gcattgggca ctgtgctagc
     1921 caacgacgag gtaagcgaag gcgtttctat ccgtggtggt catccagtag aacaacgcat
     1981 tatgcgacaa tggagtttgc gtatttctgc ttatgcacaa cgattgcttg atgggttgga
     2041 taccattgac tggtcagaat caatcaaaga aacacaacgc aactg
//
LOCUS       DAOSMY010000281         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_53883_length_2072_cov_3.894893, whole genome shotgun sequence.
ACCESSION   DAOSMY010000281 DAOSMY010000000
VERSION     DAOSMY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53883_length_2072_cov_3.894893"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLD76_06160"
     CDS             <1..625
                     /locus_tag="PLD76_06160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOU27619.1"
                     /translation="SMGDYAASGGYYIATQADSIFSNTETLTGSIGIFGIIPNFSKIS
                     EKFGITYDPQTTHTLADFPSFTRDMTTYERNILERYITDNYQLFLHRCSEGRSITKAA
                     LLPLAGGRVWTGSDALTHGLVDKIGGLQQAIQSAARMASITSYEVEHLPKKKTVWESM
                     LEGTTIQIQSLLTKNIPLHEYRLIRSFIDNKTRIIIQARMEENSEIW"
     gene            649..1743
                     /gene="lpxK"
                     /locus_tag="PLD76_06165"
     CDS             649..1743
                     /gene="lpxK"
                     /locus_tag="PLD76_06165"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOU27620.1"
                     /translation="MLLWPISWLYGISMSIRNMLFDKHILPTESFPISVISVGNITVG
                     GTGKTPHTEYLVSILKKKFNVAVLSRGYKRKTAGYIYANATASAQTIGDEPFQIYQKH
                     PDIVVAVDANRRNGIKQILLSHPQTDVVLLDDAFQHRYVEANQTILLVDYNRPITEDN
                     MLPYGNLRESIDGIKRADIILITKCPPNLTPIEARNIHKSLNLLAYQDIFYTSFAYGD
                     VKSVFTSNQIVTTSIIQEQQFGILLVTGIAQPEPLLDYICTLTPYVEKLLFPDHHFFT
                     QQDMGIIIKMFENMKQEKRLIMVTEKDAARLVNDPFFPDHLKPYTCMLPISVNFLFQQ
                     QKIFNSKIIDYVSKNQRNSNVPKRKNATLS"
     gene            1682..>2072
                     /locus_tag="PLD76_06170"
     CDS             1682..>2072
                     /locus_tag="PLD76_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU27621.1"
                     /translation="MLAKINETATYLKGKMQRFPKVGIILGTGLGELASQITEKQEIE
                     YQTIPNFPVSTVEGHSGKLIIGKLGTTEVLAMQGRFHYYEGYSMQEVTFPVRVMKALG
                     IETLLVSNASGGVNPSFEIGDLMIITDH"
BASE COUNT          735 a          391 c          364 g          582 t
ORIGIN      
        1 atcaatgggc gattatgctg cttcaggagg ctattatatt gcaactcaag ctgattctat
       61 tttcagtaat accgaaacgc ttactggatc cattggtatt tttggtatta tcccaaactt
      121 ctcaaaaatt tctgagaaat ttgggattac ttacgaccct caaacaaccc atacattagc
      181 ggactttcct tcttttacaa gagacatgac cacatatgaa cgtaacatct tggaaagata
      241 tattacagat aattatcagt tgtttttaca cagatgttct gagggtcgat ctatcacaaa
      301 agcagcatta ctaccactag ctggaggcag agtatggaca ggatcggacg ctttaacaca
      361 tggcttagta gacaaaatag gagggctaca acaagccata caatcggctg ctcgtatggc
      421 atcaattaca agctatgagg tagaacacct ccctaaaaag aaaacagtat gggaaagcat
      481 gttagaaggt acaacgatac agatccaatc tttgcttact aaaaacattc ctctgcatga
      541 gtataggtta atacgttcct tcattgacaa taaaactaga ataataatac aggctcggat
      601 ggaagaaaat agtgaaatat ggtaactcga aaaaacattt ttatacgtat gctattgtgg
      661 ccaatctcat ggctatacgg catctcaatg agtatacgca acatgctgtt tgacaaacac
      721 atattaccaa cggaaagctt tccaatcagt gtaatttctg ttggtaacat cacagtaggt
      781 ggtactggta aaactcctca tactgaatat ttagtatcta tcttaaaaaa gaaattcaat
      841 gtagcagtat taagtagagg ctacaaacgc aaaacggcag gatatatcta tgccaatgca
      901 acggcttcag cccaaacaat aggagacgag ccttttcaaa tatatcaaaa acatcctgat
      961 attgtggtag ccgttgatgc taaccgtcgt aatggaatta aacaaatttt actatctcat
     1021 ccacaaacag acgttgtact acttgatgat gcttttcaac atcgttatgt tgaagcaaat
     1081 caaacaatat tgttagttga ttataacaga cctataactg aagacaatat gttgccatat
     1141 ggtaatttac gagaatctat tgatggcatt aaacgtgcag acatcattct catcacaaaa
     1201 tgcccaccca atctaacacc tattgaagca agaaatatac ataaatcgtt aaacttatta
     1261 gcgtaccaag acattttcta tacaagcttt gcatatggtg atgtcaagag cgtatttaca
     1321 tccaaccaaa tagtaactac aagcatcatt caagaacaac agtttggaat tcttttagtt
     1381 acagggatag cacaaccaga acctctgcta gattacattt gcacactgac tccctatgta
     1441 gagaagctac tattccccga ccatcatttt tttacccaac aagatatggg aatcatcata
     1501 aagatgtttg aaaatatgaa acaggaaaaa cgcctgataa tggtaacaga aaaagatgca
     1561 gcacgtttgg taaacgaccc attttttcct gaccatctaa aaccgtacac atgtatgtta
     1621 cccatcagtg tgaatttttt atttcaacaa caaaaaatat ttaattctaa aattatagat
     1681 tatgttagca aaaatcaacg aaacagcaac gtacctaaaa ggaaaaatgc aacgctttcc
     1741 taaagtgggt attattttag gcacaggatt gggagaatta gcttctcaaa ttacagaaaa
     1801 acaagaaata gagtaccaaa caattccaaa ctttccagtt tcaacggtag aagggcatag
     1861 tggcaaattg attattggta aattaggaac aactgaagtt ctggcgatgc aaggtaggtt
     1921 ccactactac gaaggatact ctatgcaaga ggttactttc cctgtacgcg ttatgaaagc
     1981 cttaggaatt gaaacattat tagtttccaa tgccagtgga ggagtaaatc cctcatttga
     2041 aattggggat ttaatgatta tcacggatca ta
//
LOCUS       DAOSMY010000282         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_54834_length_2045_cov_3.467337, whole genome shotgun sequence.
ACCESSION   DAOSMY010000282 DAOSMY010000000
VERSION     DAOSMY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54834_length_2045_cov_3.467337"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLD76_06175"
     CDS             <1..297
                     /locus_tag="PLD76_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOU27622.1"
                     /translation="YISGQIPVNPENGKVDALTIEQQAEQVLQNIGAILQAAGYGYSD
                     VVKSTIFITDMKNFSAVNEVYKKYYTEKFPARSTVAVKELPLGVMVEIETIAVK"
     gene            complement(307..>2045)
                     /locus_tag="PLD76_06180"
     CDS             complement(307..>2045)
                     /locus_tag="PLD76_06180"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HOU27623.1"
                     /translation="SALLIGHRLCKDEKGIFVEHTTKKVSIGDSVRITATLTNNNNRL
                     QLTNVSALDIVNSNNQIPITKLIFPDDFDSLEAWEGTLVELDQTMYVTNNYNLFRYGQ
                     LTLSANRLYIPTHQALPLSDVYFQTISSNEINQLILDDASSVSYPTNKPFTDSNGTRR
                     MGEKIDNLQVIIDQVGTNYVVYAPKDIDFYGNPRTATHPSIGDYSIKVCGTNLEMFKT
                     SYWGDGFGADNSTEFARQRTKILAALHAINADIYAITEIQQGSNALENLVNGLNALRG
                     NTDFTYINDKDNVSEYIKSAFIYNKNKVTPHLNVINNNTISSTRFRKKAQAFIENSSK
                     EKFIVVVNHYKAKSGCPSSGADADKQDGQGCYNATRTQEAVQSLTFINYMINYAKDTD
                     VLFLGDLNSYKYEDPITTLRNGGLEDLLEHFQGNDAYSYVYGGKAGYLDYALANSSML
                     RQATGASPWHLNSDEPSQLSYDNATYYQPNMYRYSDHDPIVVGLKLTPQAPFEPLDKI
                     QLFHDLTSKHIIVTRAKNHQVSIIGIDGRFAHFHSIESDTETINTNMLPAGVYVVKIT
                     GTKEETFKFIQL"
BASE COUNT          604 a          353 c          384 g          704 t
ORIGIN      
        1 tatatttctg gtcaaatacc tgtaaaccca gaaaatggta aagttgatgc tcttacaata
       61 gagcaacaag cagagcaagt acttcaaaat attggagcca ttttgcaagc agctggctac
      121 ggctattcag atgtagtaaa atccactatt tttataacag acatgaagaa tttttcggct
      181 gtaaacgaag tgtacaaaaa atattatact gaaaaatttc ctgctcgttc aactgtagct
      241 gtaaaagaac taccattggg ggtaatggta gaaattgaaa cgattgccgt aaaatagggt
      301 agtattctat aattgaataa acttaaacgt ttcttctttc gttcctgtga tttttacaac
      361 ataaacacca gctggcaaca tgttggtgtt tattgtttct gtatccgatt caattgagtg
      421 gaagtgagca aaacgtccat cgataccaat aatagacact tggtgatttt ttgcacgtgt
      481 tacaatgata tgtttggatg tcaaatcgtg aaataattga attttatcca agggttcaaa
      541 tggagcttgt ggtgttagct tcaaaccaac tacaataggg tcgtggtcgg aatacctgta
      601 catatttggt tgataatagg tggcattatc atatgacaat tgcgatggct catcgctatt
      661 taagtgccag ggactagctc cagttgcttg acgcaacata ctgctgtttg ctagtgcata
      721 atccaaataa cctgcttttc ctccatacac atacgaataa gcatcgtttc cttgaaaatg
      781 ctctagtaaa tcttccaatc ctccattgcg taaggtagtg attggatctt cgtacttgta
      841 agaatttaag tcaccaagaa atagaacgtc tgtatctttg gcataattaa tcatataatt
      901 gataaacgtt aaagactgaa ccgcttcttg tgtacgagta gcattgtagc acccttgacc
      961 gtcttgctta tcagcatctg cccccgatga tgggcatccg cttttggctt tatagtgatt
     1021 aacaacaacg ataaattttt ctttggagct gttttcgata aaagcttgtg ctttttttct
     1081 aaaacgggta cttgatattg tattgttatt gattacattt aagtgtggtg ttaccttatt
     1141 tttgttataa ataaaagcag attttatgta ttcagacaca ttgtccttat cattaatata
     1201 ggtaaaatca gtgtttccac gtagtgcatt aagtccattc actaggtttt ccaatgcgtt
     1261 tgacccttgc tgaatttctg ttatggcata aatatctgca tttattgcat gcaaagctgc
     1321 taaaattttt gttcgttggc gtgcaaactc ggtactatta tcagctccaa acccatctcc
     1381 ccaataagac gttttaaaca tttctaaatt tgtgccacaa acttttattg aataatctcc
     1441 aattgatgga tgcgttgctg tacgtggatt gccataaaaa tcaatgtctt tcggagcata
     1501 cactacataa tttgttccaa cttggtcgat aatgacttgc aagttatcaa ttttttcacc
     1561 cattcgtcgt gtgccattgc tatctgtaaa aggcttattt gtaggatatg atacagaaga
     1621 tgcatcatct agtattagtt ggttaatttc gttactcgaa atagtttgaa aatacacatc
     1681 acttaatggg agtgcttgat gcgttggaat gtataaacgg ttagcagata aggtaagctg
     1741 tccgtatcga aaaaggttgt agttgttggt aacatacatg gtttggtcta attcaacgag
     1801 agttccttcc catgcttcca gtgaatcaaa atcatcagga aaaattaatt ttgtaatggg
     1861 tatttggttg tttgagttta caatatctaa agcagataca ttcgttaatt gtaatctgtt
     1921 attgttgtta gtgagtgtgg cagttatacg tacactatct ccaatgctta ctttttttgt
     1981 ggtatgttca acaaaaattc ctttttcatc tttgcataaa cggtgtccga ttagcaacgc
     2041 actca
//
LOCUS       DAOSMY010000283         2030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_55401_length_2030_cov_3.465316, whole genome shotgun sequence.
ACCESSION   DAOSMY010000283 DAOSMY010000000
VERSION     DAOSMY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55401_length_2030_cov_3.465316"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..76
                     /locus_tag="PLD76_06185"
     CDS             <1..76
                     /locus_tag="PLD76_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU27624.1"
                     /translation="DVDGGLIGGASLKAVDFKAIISAF"
     gene            198..788
                     /locus_tag="PLD76_06190"
     CDS             198..788
                     /locus_tag="PLD76_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOU27625.1"
                     /translation="MKHTFIFATNNKHKLEEIQQIVGDKLSILSLQDMNFREDIPENE
                     PTIEGNALYKARFIYNRFGKDCFADDTGLEVVALNGEPGVLSARYAGEEKNYQSNNEL
                     LLKNLNPFPNKSARFKTVIACVLEGREYVFEGIIEGTIVNEPKGKNGFGYDPIFQPSG
                     HQQTFAELSEEVKNTISHRARAMESLFMFLQQNHII"
     gene            814..>2030
                     /locus_tag="PLD76_06195"
     CDS             814..>2030
                     /locus_tag="PLD76_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27626.1"
                     /translation="MRKIIFLFPFLFFSSIVFSQIAVGEWRMHFSYNSTTNVVVTPKK
                     VYAIADNKLFSVDKKDFSIQTYSKLSGLNSNEVSLLAYDEISNLLIVVYTNSNIDIID
                     QKGGIYNIADLYRKNMTVSKKVNHIYFDQGLAYFSCDFGIMVLNLAKKEIANTYIIGD
                     NATMSAVYAVNSDETYLYALTSESIKKAPKQGVNLLNFENWLVAMSLPDDSQLFRDMD
                     VFNNKFYVAKNNENVYVYEEGQWLVFYANDAQLAISIQTIGNHFIINASNSLLCYTKD
                     LTVEQLTDIVVSDSWYDDTEGVYWIASKTSALLRYKKGEGVLGRYKPSSPYLSTAQKM
                     YYNNGRILVAPGKSWDDRYSIPGAVLLFEDEKWTEYTSENSGVLQYSYWFADVVSIAV
                     DPVDKNKLYVSTWG"
BASE COUNT          675 a          244 c          413 g          698 t
ORIGIN      
        1 agatgtagat ggaggattaa ttggtggagc atcgcttaaa gcagttgact ttaaagcaat
       61 tattagtgcg ttttaattgg ttttggtaat taaatttaag ccatcctttt gtaaggatgg
      121 cttttttgtc taaatagttt tataaatccg aatataaagg tatttttgta aggtaaaact
      181 tgatattttg aattaggatg aaacatacat ttatttttgc tacaaataat aaacataaat
      241 tagaagagat tcagcaaata gttggggata aattatcaat cttgagttta caagatatga
      301 actttagaga ggatattcca gagaatgaac ctactatcga agggaatgca ttgtataaag
      361 ctcgatttat ttacaatcgt tttggcaaag attgttttgc tgatgatacg ggtttggagg
      421 tagttgcgtt gaatggtgaa cctggcgttt tatcagcacg ttatgcggga gaagaaaaaa
      481 attatcagtc caataatgaa ttattgctaa aaaatcttaa cccatttccg aataagtcag
      541 cacgttttaa aactgtgatt gcatgtgtgt tagagggaag agagtatgtg tttgagggta
      601 taattgaggg tactattgtt aatgaaccaa aagggaaaaa tggatttgga tatgatccga
      661 tttttcaacc atctggacat caacagactt ttgcagaatt atctgaagaa gttaaaaata
      721 caattagtca tcgggctaga gccatggaaa gtttatttat gtttttgcaa caaaatcata
      781 ttatttaaat caaagcaaat aaatctttat agtatgcgaa aaatcatctt tttgtttcca
      841 tttctatttt tttctagtat cgtattctct cagattgctg tgggagagtg gcgtatgcac
      901 ttttcatata attctacgac caatgttgta gtaacaccta agaaagtgta tgctatagca
      961 gataataaat tgttttcggt ggacaaaaaa gatttttcta ttcaaaccta ttctaaactg
     1021 tcgggattga actcaaatga agtttcgttg ttagcttatg atgagattag taatttgcta
     1081 atagtggtat atacaaactc caatatagat attattgatc agaagggagg tatctataat
     1141 attgctgatt tgtatcgaaa aaatatgaca gtaagcaaaa aagttaatca tatttatttt
     1201 gatcaaggtt tagcgtactt ttcgtgtgat tttggaatta tggtattaaa cttagctaaa
     1261 aaggaaattg ccaataccta tatcattgga gataatgcca ctatgtctgc agtatatgct
     1321 gtaaattccg atgaaacgta tttatatgca cttaccagtg aatctattaa aaaagcacct
     1381 aaacaaggtg taaatttatt gaattttgaa aattggttgg ttgctatgag tttgcctgat
     1441 gattcgcagt tatttagaga tatggatgta tttaataata agttttatgt cgccaaaaat
     1501 aatgagaatg tatatgtgta tgaagaaggt caatggctag tgttttacgc taatgatgca
     1561 cagctagcta tttctattca aacgataggt aaccatttta ttataaatgc ttccaattcc
     1621 ttgttgtgtt atacgaaaga tttaaccgta gaacaactga ctgacatagt agtatctgat
     1681 tcgtggtacg atgatacaga aggtgtttat tggattgcat caaagacatc agcacttctt
     1741 cggtataaaa aaggggaagg ggtattaggt agatataagc cgtcatctcc gtatttaagt
     1801 actgctcaaa agatgtatta taacaatggt agaatattgg ttgctcctgg caaaagttgg
     1861 gatgatagat atagtatacc tggagcagtt ttattatttg aggatgaaaa atggactgaa
     1921 tatacatctg agaattcggg agtgttacag tattcttatt ggtttgcaga tgtggtttct
     1981 atagcagttg atcctgtgga taagaataaa ttatatgtta gtacatgggg
//
LOCUS       DAOSMY010000284         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_55466_length_2028_cov_3.004562, whole genome shotgun sequence.
ACCESSION   DAOSMY010000284 DAOSMY010000000
VERSION     DAOSMY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55466_length_2028_cov_3.004562"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLD76_06200"
     CDS             complement(<1..1043)
                     /locus_tag="PLD76_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOU27627.1"
                     /translation="MENEMNYSDNVADILRYSHEEAERLGNNYIGPEHLLLAIIRAGE
                     GKAFDVLLSLNLDLLRIKKTIESSIRTSENVMIGSNIPLLKSTEKILKFIVLEARQFG
                     NYMADSEHLLLAILKDKTNLVTEIFSVENISYDTVRALVEKKDIGSIDKKNGVNSNDE
                     DGEDEDSEFSSNSVEKNGKTSTFSTSTKYTKNTSKTPALDNFGTDITKAAEEGKLDPI
                     VGREKEIERLSQILSRRKKNNPILIGDPGVGKSAIVEGLALRIIQRKVSRILFGKRVV
                     PLDMASIVAGTKYRGQFEERLKAILNELETNPDVILFIDEIHTLIGAGATAGSLDAAN
                     MMKPALARGMIQC"
     gene            1299..>2028
                     /locus_tag="PLD76_06205"
     CDS             1299..>2028
                     /locus_tag="PLD76_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOU27628.1"
                     /translation="MIDQERIIKINIEEEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMNELGSNSNKPYKKSARIVGEVMGKYHPHGDSSIYGTLVRMAQEWSMRYQLV
                     DGQGNFGSIDADEPAAMRYTEARMHRLAEEMLMDIDKDTVDFKLNFDDTLKEPTVLPT
                     RIPNLLINGASGIAVGMATNMAPHNLSDTIDAIVAYIDNKEVDDEELIKLIKAPDFPT
                     GGIIYGYAGIRDAYLTGKGRVVIRG"
BASE COUNT          594 a          335 c          381 g          718 t
ORIGIN      
        1 atgcattgta tcatgcctcg agccaaagct ggtttcatca tgtttgcagc atctaacgag
       61 cctgctgttg caccagcacc aattaaggta tgtatttcgt cgatgaataa aataacatca
      121 gggtttgttt ccaattcatt taagattgct tttaatcttt cttcaaactg tcctctatac
      181 tttgttcctg ctacaatgga tgccatatct aacggtacta cacgtttgcc gaataatata
      241 cgagatactt ttcgttgtat aatacgtagt gccaatcctt caacaatagc cgattttcca
      301 acacctgggt cgccaatgag tataggatta ttcttttttc ttctacttaa aatttgagat
      361 aggcgttcaa tttctttttc tctaccaacg attgggtcta actttccttc ttcggctgct
      421 ttggtaatgt ctgtaccaaa attgtcgagt gcaggtgttt tagaagtgtt ttttgtgtat
      481 ttagttgatg tagaaaaggt gcttgtttta ccattttttt ctacagaatt tgagctaaat
      541 tcactatctt cgtcttcgcc atcttcgtca ttcgagttta ctccgttttt tttatcaata
      601 gatccaatgt cttttttttc tactaaagct cttactgtat catacgaaat gttttctaca
      661 ctaaaaattt ctgtaaccag atttgtttta tcttttaata ttgctagtaa aaggtgctca
      721 gaatcagcca tatagttgcc aaattgacga gcctcaagta caatgaattt gagtattttt
      781 tcagttgatt ttagcagggg tatattggat ccaatcatca cgttttctga tgttcggata
      841 ctcgattcaa ttgttttttt tatacgcaat aaatctaagt taagtgacaa aagtacgtca
      901 aaagctttac cctctcctgc acgaataatt gccaaaagta agtgttcggg tccaatgtaa
      961 ttattgccca aacgttctgc ttcttcgtgg ctatatcgta aaatatcggc aacattgtcc
     1021 gaatagttca tttcattttc catattctta cgtgtttgat aaacattatt gttagtgttt
     1081 gctaactatt ttgcacatat tgtattgcaa aggtatagtt tttctttaaa aattgtgtat
     1141 tgtttacaca ttcttaacaa cagattacaa atactatgcc aacaatatat gtgtgtgtta
     1201 aaaagatttt tgacgaaagg tttttgtatt tgaataaaaa gtagtacctt agtgcgaatt
     1261 ttcagaaaag gtatttgttt tacttaacta ttttataaat gattgatcaa gaaaggatta
     1321 taaaaatcaa cattgaggag gagatgaagt cagcatacat tgattactca atgtctgtta
     1381 ttgtatcgcg tgcacttccc gatgtaagag atggattgaa gcctgtacat cgtagagtat
     1441 tgttcggaat gaatgagttg gggagtaact ccaataaacc gtataaaaaa tctgctagaa
     1501 tagtagggga agtgatgggt aagtatcacc ctcatggcga ttcttctatt tatggtacat
     1561 tagtgcgaat ggcacaagaa tggtcaatgc gttatcaact ggttgatggt cagggaaact
     1621 ttggctctat tgatgccgac gaacctgctg ctatgcgtta tacggaagcc cgtatgcatc
     1681 gcttggcaga agaaatgttg atggatattg ataaagatac cgttgatttt aaacttaatt
     1741 ttgacgatac attaaaagaa ccaactgttc tgccaactcg tatacccaat ttattgataa
     1801 atggagcatc gggaattgca gtaggtatgg caaccaatat ggctcctcat aatttatctg
     1861 acacgataga tgccattgtg gcatatattg acaacaaaga ggtagatgat gaagagttaa
     1921 tcaaattaat taaagctcca gattttccaa caggaggtat tatttatggt tatgcgggta
     1981 ttagagatgc ttatttaaca ggtaaaggtc gtgttgttat aagaggta
//
LOCUS       DAOSMY010000285         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_56431_length_2003_cov_2.992300, whole genome shotgun sequence.
ACCESSION   DAOSMY010000285 DAOSMY010000000
VERSION     DAOSMY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56431_length_2003_cov_2.992300"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLD76_06210"
     CDS             <1..528
                     /locus_tag="PLD76_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU27629.1"
                     /translation="NFLSITSKPFIYQEGDANNSSFDLASIPYGSDSFDPSGVGGVGG
                     MGNTSNVGGINGGRFAGREISINEIYYDFDNANLLPQSRFSLDKIILLLSSNPTLKLE
                     FGSHTDTRGSAEYNLQLSNARAKSVVDYLIASGISRNRISWKGYGKTNPIVKNAVTEE
                     EHRLNRRTTFKIIER"
     gene            546..1472
                     /locus_tag="PLD76_06215"
     CDS             546..1472
                     /locus_tag="PLD76_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU27630.1"
                     /translation="MKKILIACCASLLFSLSVFAQHDAQISQHLLSRTHYNPAAAGAS
                     LYGNITGIARQQYVGFTGAPQTGLLNFDMLLPNTSSGIGLSVMYDKYGPLLSYNPMLN
                     YAYHIKLGDNQHLSLGVAAGVFLRQYDASNNIYENIGDPLENYDGAQEYLAPDANVGI
                     EYQLENWIIGASATHIPQFFEEETVMNPTTQYYAYSRYRFEIGRYWDLTPGIAAQYDG
                     YSVHSEINAVLHYLKLFWVGGSYRFSDVFDSESIVPMVGLQLSDYVRLGYAYDYNLSS
                     LQNYNQGSHEIMLQLRFKTRHHTTRTPRFAEW"
     gene            1607..>2003
                     /locus_tag="PLD76_06220"
     CDS             1607..>2003
                     /locus_tag="PLD76_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU27631.1"
                     /translation="MADILDTKEQAENELIEQEYQLLIKEYLASNHRKKVARIEKAFL
                     LAKAAHKGIKRKSGEPYILHPLAVARIVAKEIGLGSTSICAALLHDVVEDTEYTVEDI
                     ANLFDPKIAQIVDGLTKISGGVFGENVSGQ"
BASE COUNT          655 a          361 c          398 g          589 t
ORIGIN      
        1 aacttcttgt ctattacaag taaacctttt atttaccaag aaggagatgc aaacaatagc
       61 tcgttcgatt tagcttccat tccttacggt tcagattcgt ttgatccaag tggtgttggt
      121 ggtgttggtg gtatgggtaa tacaagcaat gttggaggta taaatggagg acgatttgct
      181 ggtcgcgaaa tttcaataaa cgaaatttat tacgatttcg acaacgctaa cttgcttcca
      241 caatcacgtt tttcattaga caaaattatt ttattattaa gctctaaccc aaccttaaaa
      301 cttgaatttg gttcgcatac tgacacacgt ggtagtgctg aatacaacct acaattatct
      361 aatgcacgtg caaaatctgt ggtggactat ttaattgctt cgggcattag tcgcaacaga
      421 atttcgtgga aaggatatgg taaaaccaat cctatagtga aaaatgctgt tacagaagaa
      481 gaacatcgat taaacagacg taccacattc aaaattattg aacgataact ttaaacatga
      541 acacaatgaa aaaaatactt atagcttgct gtgcatccct tctgttttct ttgtcggttt
      601 ttgcacaaca cgacgcacaa atcagtcaac atttgctgag tagaacgcat tacaaccctg
      661 ctgcagcagg agcttcgtta tacggtaaca ttacaggtat tgctcgtcaa cagtatgtgg
      721 gctttacagg agcaccgcaa actggcttgc taaactttga catgttactc ccaaatacca
      781 gctctggtat agggttatca gtaatgtatg ataagtatgg accgctgtta tcgtataatc
      841 caatgcttaa ctatgcgtat catattaaat tgggcgacaa ccaacatctt tcgttaggag
      901 tagccgcagg ggtgttttta cgccaatatg atgcatcaaa caatatttac gaaaatattg
      961 gagacccttt agaaaattat gatggagctc aagaatactt agcaccagat gccaacgtag
     1021 gtatcgaata ccaattagaa aactggatta ttggggcatc agctacccat atacctcaat
     1081 tttttgaaga agaaaccgta atgaatccca caacacagta ttatgcctac tcgcgttatc
     1141 gctttgaaat tggcaggtac tgggatttaa ctcctggcat tgcagctcaa tacgatggct
     1201 actctgtaca tagcgaaata aatgcggtat tgcattacct aaaattattt tgggtaggtg
     1261 gctcgtaccg atttagcgat gtattcgatt ctgaatcaat tgtgcctatg gtaggacttc
     1321 aattaagcga ctatgtacgc ttaggatatg cgtacgatta caatttatcg agcttacaaa
     1381 actacaacca ggggtcgcac gaaattatgc tacaattacg tttcaaaaca cggcaccata
     1441 caacacgaac accgagattt gctgaatggt aagaaagtag aatacatttt aactcaaaaa
     1501 gacgctgaaa ataacacagc gtcttttttt ttgtaaaaca agactgtaaa aatgatacat
     1561 aaataaaaaa agcattgtat atttgtgtta ttaagaaaaa cgatatatgg cagatatttt
     1621 agatacgaaa gaacaagcag aaaatgaact tattgagcaa gaatatcagt tacttattaa
     1681 agaatatctt gcctctaacc atagaaaaaa agttgctcgt atagaaaaag cttttttact
     1741 ggctaaagct gctcataaag gaataaagcg taaatcgggg gagccttaca tcttacaccc
     1801 tttggctgta gcacgcattg ttgctaagga gattggactc ggttctacgt ctatttgtgc
     1861 cgcgttgttg catgatgtgg tagaagatac tgaatatacc gtagaagata ttgcgaattt
     1921 gtttgacccc aagattgcac aaattgtaga tgggttaacc aaaatatcag gtggtgtttt
     1981 tggtgaaaat gtgtcgggac agg
//
LOCUS       DAOSMY010000286         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_56771_length_1994_cov_3.440949, whole genome shotgun sequence.
ACCESSION   DAOSMY010000286 DAOSMY010000000
VERSION     DAOSMY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56771_length_1994_cov_3.440949"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1181
                     /locus_tag="PLD76_06225"
     CDS             3..1181
                     /locus_tag="PLD76_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOU27632.1"
                     /translation="MYIAGFFSEKAKLLYKGQRNTLWLAENSIDKNQPYIWVHAASLG
                     EFEQGRPLIEAIKKDHPTYKIVLSFFSPSGYEVRKDYALADYVCYLPLDTKANATRFI
                     ELIQPVKAIFIKYEFWPNYLQVLRKKGIETYIVSAIFRKEQIFFSWYGGWYKKLLQCF
                     THIFVQDTSSFELLQTHGIHRVSIAGDTRFDRVATIAASPTQLPIIESFKQNKKLIVL
                     GSSWEPDEALLIPFVEKNDCKLIIAPHQIHTERIQSLLAKLSMPTALYTQTTAEDVKH
                     ANCLVINTIGILSSVYRYADVAYIGGGFGVGIHNILEAAVFGVPVLFGPNYHKFKEAV
                     DLINEGGAFCIQTQTDIDSIGKNLLTDNAFYQQVCTQSASFVQHRIGATQGIMNQLFV
                     "
     gene            1185..>1994
                     /locus_tag="PLD76_06230"
     CDS             1185..>1994
                     /locus_tag="PLD76_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOU27633.1"
                     /translation="MKVKLVLLSMMLFSLLVATQAQIAPKREFRGVWMHTVFSDDYAQ
                     KSNEQIKNFLLQRLNEYQSVGMNVLIFQVRPEADAFYKSSYEPWSRFLTGKQGKAPAD
                     SFDPMTFLIDECHKRGIEFHAWLNPYRANANRKNELHPDHLYWKRKDLFVYFGNQLFF
                     NPGIPENRTHIAKVVKDIVARYDVDAIHMDDYFYPYPIKGSSFPDQKEFVKYGLQTGE
                     FKLYQKDDWRRNNVNLLIKELNTVIKKEKPWVKFGISPFGIYRNQRQYAEGS"
BASE COUNT          660 a          411 c          371 g          552 t
ORIGIN      
        1 tcatttatat agctgggttt ttctcggaaa aagcaaaact gctctacaag gggcagcgaa
       61 acaccctttg gttagctgaa aatagtatag acaaaaatca gccctatatc tgggtgcatg
      121 cagcttcgtt gggcgaattt gaacaaggaa gaccccttat cgaagccata aaaaaagacc
      181 atccaacgta caaaatcgta cttagttttt tttccccctc tggctacgaa gtaagaaaag
      241 attatgcact agcagattat gtatgctact taccactcga taccaaagcc aatgctacac
      301 gctttattga gctaatacaa ccagtaaaag cgatttttat caaatacgaa ttttggccca
      361 attacttgca ggtattacgc aaaaaaggca tcgaaacata cattgtttcg gcaatatttc
      421 gcaaagaaca aatctttttt tcgtggtatg gtggctggta caaaaaacta ttacagtgtt
      481 ttacccatat ctttgtacaa gataccagtt cttttgagct acttcaaact catggcatac
      541 accgagtaag tattgccggc gacacccgtt ttgacagggt agctactatt gcagcttcac
      601 ctacgcaact acctattatt gaatctttta aacaaaataa aaaacttatc gttttgggta
      661 gttcgtggga gccagacgaa gccctactta taccgtttgt ggagaaaaac gattgcaaac
      721 tgataattgc accgcatcaa attcacaccg aacgcataca aagtttactt gcaaaactat
      781 ctatgccaac ggctctctat acccaaacca cagcagagga tgtaaaacat gccaattgtt
      841 tagtaatcaa tacaattggt atattgtcat cggtatatcg ctatgcagat gtagcgtata
      901 ttggtggagg gtttggcgta ggtattcaca atatcttaga ggcggctgta tttggtgttc
      961 ctgtactctt tggtccgaat taccacaagt ttaaagaagc tgtcgacttg ataaacgaag
     1021 gtggagcttt ctgtatacaa acgcaaacag atatagattc aattggcaag aatctactca
     1081 cagacaatgc cttttaccaa caagtatgta cccaatccgc ttcgtttgta cagcacagaa
     1141 taggagcaac acaaggtatt atgaaccaac tatttgtata aaatatgaaa gtaaaactag
     1201 tactactttc aatgatgctc ttttctctat tggtggcaac tcaagctcaa attgccccta
     1261 aacgcgaatt tagaggcgta tggatgcata cggtatttag cgacgattat gctcaaaaaa
     1321 gcaacgaaca gataaaaaat ttcttacttc aacgcctcaa cgaataccaa tcggtaggca
     1381 tgaatgtact gatatttcaa gtacgccccg aagccgatgc tttttacaaa tcgtcttacg
     1441 aaccatggag ccgttttctt acgggcaaac aaggaaaagc tcctgccgat agtttcgacc
     1501 caatgacttt tttaattgac gaatgccaca aaagagggat agagtttcat gcatggctca
     1561 acccatatcg tgccaatgca aaccgcaaaa acgaactgca ccccgatcac ttatattgga
     1621 aacgaaaaga tttatttgtg tattttggca accaattgtt ttttaatccc ggtatacccg
     1681 aaaaccgcac ccatatagcc aaagtagtaa aagacattgt agctcgctat gatgtagatg
     1741 cgatacacat ggacgattat ttttatcctt atccgattaa aggaagttcg tttcccgacc
     1801 aaaaagaatt tgttaaatac ggcttacaaa caggagaatt taaactgtac caaaaagacg
     1861 actggcgtag aaataatgtc aatttactga ttaaagaact gaatacggtt atcaaaaaag
     1921 aaaaaccttg ggtaaaattt ggcattagtc cttttggtat ctaccgcaac caacgtcaat
     1981 atgccgaagg tagc
//
LOCUS       DAOSMY010000287         1980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_57317_length_1980_cov_4.099221, whole genome shotgun sequence.
ACCESSION   DAOSMY010000287 DAOSMY010000000
VERSION     DAOSMY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57317_length_1980_cov_4.099221"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLD76_06235"
     CDS             <1..252
                     /locus_tag="PLD76_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOU27634.1"
                     /translation="HSVKADAITQQVFENDEAKVYGVLYEIKGSVASSLQFTLTDSTT
                     HYFRGAVYFNNRPNNDSIAPVLSYIREDVVRMMETMQWK"
     gene            266..877
                     /locus_tag="PLD76_06240"
     CDS             266..877
                     /locus_tag="PLD76_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOU27635.1"
                     /translation="MPILDNIHTPSIDVAIWHITETADELFSMLLPNPVYTNELNRIS
                     SNKRKSEFLAVRLLMQHLVGDSTIAYLSNGKPILSGSSRFISISHTKEYVAVAISNQH
                     EVGIDIETISAKVHQVKERFMNETELVHAQQYPEFVLLSWVAKEAAYKKIGDGVVHFS
                     QSMELVPFVPHLSGVLELKAQLLEETRYSFYYQQHTDFVLVYG"
     gene            969..1042
                     /locus_tag="PLD76_06245"
     tRNA            969..1042
                     /locus_tag="PLD76_06245"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1003..1005,aa:Asp,seq:gtc)
     gene            complement(1250..1429)
                     /locus_tag="PLD76_06250"
     CDS             complement(1250..1429)
                     /locus_tag="PLD76_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU27636.1"
                     /translation="MEEDIIICHCNEIYKSEIVKAIKEKGLTTVDEVGDETSAGTICG
                     QCQDDIQDILDEINK"
     gene            complement(1550..>1980)
                     /locus_tag="PLD76_06255"
     CDS             complement(1550..>1980)
                     /locus_tag="PLD76_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU27637.1"
                     /translation="EIVIDSHLQEISKEKIVFLQDNANNLKPIHSGFDVIIANNVLEE
                     LNNPIQFLQTIHQRLHKQGILIVASSYNWKKNNIPAENWLGGYKKDGEPVTGLDAIKE
                     NLQNTFVLLDKPFDLQKIAYISTRESSVQRCEVSIWQKKS"
BASE COUNT          582 a          367 c          354 g          677 t
ORIGIN      
        1 cattcagtaa aagcagatgc cattacccag caagtttttg aaaatgacga agcaaaggta
       61 tatggggtgt tgtacgaaat taagggcagc gtagcctctt ccttgcagtt tactctgact
      121 gatagtacta cccattattt tagaggtgct gtctatttta acaaccgacc caataacgat
      181 tctattgctc ccgttttgtc ttacattcgc gaagatgtag tacgcatgat ggaaaccatg
      241 caatggaaat aatatttcga ccaaagtgcc tattcttgat aacatacaca cacctagcat
      301 agatgtagct atttggcata taaccgaaac tgctgacgag ctgttttcga tgctattgcc
      361 caatcctgtg tatacaaacg aacttaacag gatatctagc aataaacgaa agtccgaatt
      421 tttggcagtg cgtttattaa tgcaacatct agtaggggat agcactattg cctatttatc
      481 gaatggtaaa cctattttat ctggttcgtc gcgttttatt agtatttcgc atacaaaaga
      541 gtatgttgca gttgccataa gcaatcaaca tgaagtaggc attgatattg aaacaatttc
      601 tgccaaggta catcaggtaa aagaacgttt tatgaacgaa acagagcttg tacatgctca
      661 acagtacccc gaatttgtct tgttatcttg ggtagctaaa gaggctgcat acaaaaagat
      721 aggcgacggg gtggtgcatt tttcccagtc tatggagctt gtgccgtttg ttccgcattt
      781 atcaggcgtg ttggagttaa aggcacaatt acttgaagaa acacggtatt ccttttatta
      841 tcagcaacat actgactttg tattggtata tgggtaattt tttgtcgatt ttatcaaaaa
      901 aataccatca acacttgtat aatataaaaa atcagcatat ctttgcatcg caattgagcg
      961 aaacacatgg tgtggtagtt cagctggtta gaatacctgc ctgtcacgca gggggtcgcg
     1021 ggttcgagtc ccgtccatac cgctaaatag cctgttaatc atctgattat caggctattt
     1081 tgtttttatg tgtcgcattt gccgtgcagt ggtgctgaaa atatagaata ctcaattgga
     1141 ctgtctcatc caaaaatacc tctccaaaaa agtcaatatt gaaaacataa acttttataa
     1201 accaaaatag ccatcaaact tgatggctat ttttagtaga atagtttttt tacttgttta
     1261 tttcatccaa aatatcttgg atatcgtctt ggcactgacc gcaaatagtg cctgctgagg
     1321 tttcgtcgcc cacttcatct acggtagtta agcctttttc tttaatcgct tttactattt
     1381 cgcttttgta aatctcatta caatggcaaa tgattatatc ttcttccata atggtttcca
     1441 gttaaattag taatttcttt cattcactct aaaaacaaag gtatagcaaa gtgattaaaa
     1501 atcgcaatta ttttagttaa tttactttat atactgttta taaaacttac tagctttttt
     1561 tctgccatat acttacttca catcgttgaa cgcttgattc tcttgtagaa atatacgcaa
     1621 ttttttgcaa atcaaaaggt ttatcaagca gaacaaaggt attttgcaga ttttctttta
     1681 tggcatccag tcctgttaca ggttcgccat cttttttgta gccacccaac caattttctg
     1741 caggtatgtt attttttttc caattatagc ttgaagccac tatcaatatg ccttgcttat
     1801 gtaatcgttg atgaatggtt tgcaaaaact ggatagggtt attcaattct tccaatacat
     1861 tgttggctat tattacatca aatccactgt gaattggttt aaggttgttg gcattgtctt
     1921 gcaaaaacac aattttttct ttggaaattt cttgcaaatg gctgtcaatt acaatttcgc
//
LOCUS       DAOSMY010000288         1975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_57542_length_1975_cov_2.644792, whole genome shotgun sequence.
ACCESSION   DAOSMY010000288 DAOSMY010000000
VERSION     DAOSMY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57542_length_1975_cov_2.644792"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1494)
                     /locus_tag="PLD76_06260"
     CDS             complement(160..1494)
                     /locus_tag="PLD76_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27638.1"
                     /translation="MKQLDNANERFAQLLKNYESNLTGGYFDAEEMEQLADFYLRRMR
                     VKDAKRAIEFGLKLHPNNIGLETSKVRILIELNDLELALQHTNSLVALYPSDIELHLL
                     QTEILLKTKQEVQAEKNCKELINTYAQEDETIYLDIAYVYLNNEYYQKALDFLLIGHT
                     RNVYNINILFEIAFCYEKLNQIDNSIATYHRILDIEPYSDEAWFNLGQVFYSQKDYAK
                     AIEAYEYATAINEEDAMAWQLLAHAYYQNDNYKKAAETYLMYAEQTEHKATPLLFAAE
                     SYEKMEDFDLALHYYSMVLDMQPNNTEAMVGVGICHLEKEQFQKSINYFVRAIELDPT
                     LSEAWVYLGEAYINLDDPSKGLQAYLTSIELNSNQPETLTSIGNLYLEQENFSMALHY
                     YLTAFELDHETDNIHLFLAMAYYGEENYLPAKEHLQKAININTEALNLFLEI"
     gene            complement(1590..>1975)
                     /locus_tag="PLD76_06265"
     CDS             complement(1590..>1975)
                     /locus_tag="PLD76_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU27639.1"
                     /translation="ILSTVVDSSQNILLLSNDVDVIGIDEAQFFDMGLIDVCRQLANQ
                     GMRVIIAGLDMDFKGNPFGPMPALCAIAEHVTKVRAICVKCGHLALYSYRLTNSDKQV
                     LLGETADYQPLCRECYHESTTKLKE"
BASE COUNT          585 a          326 c          333 g          731 t
ORIGIN      
        1 ttttgagtat gaatgatgta atggatttcc tataacaccg tattgtttca taatggtagt
       61 ttatttttgt tttattttta gtatgcgtat gtacgtttat taatttttgc aataatccag
      121 ctactaggtt gttagattaa tgtattgtag tgcatctggc tatatttcta gaaataaatt
      181 taatgcttca gtatttatat tgatagcttt ttgtagatgt tctttggctg gcagataatt
      241 ttcttcaccg taatacgcca tagcaagaaa taagtgtata ttgtctgttt catgatctaa
      301 ttcaaacgct gttaaatagt aatgtaatgc cattgaaaag ttttcttgtt ccaaatataa
      361 atttccaatg ctagttaatg tttcgggttg gttgctgttt aactcgatag aagttaaata
      421 ggcttgtagc ccttttgaag ggtcatctaa attgatatat gcttcgccta aatataccca
      481 agcttcactt agtgttggat ctaattcaat ggcacgtaca aaataattga tgcttttttg
      541 aaattgttct ttttccaaat ggcaaatacc tacacctacc attgcctcag tattgtttgg
      601 ttgcatatct agtaccatac tgtaataatg caaagctaaa tcaaaatctt ccattttttc
      661 ataactttcg gctgcaaata gtagtggtgt agctttgtgt tctgtttgct cagcatacat
      721 taaataggtt tcggctgctt ttttgtagtt atcgttttgg taataagcat gtgccaatag
      781 ttgccatgcc atagcatctt cttcatttat agcggtagca tattcgtatg cctctatggc
      841 ttttgcatag tctttttgag aataaaagac ctgccccaaa ttaaaccaag cttcgtcgga
      901 atacggttca atgtctaaaa tacgatggta agttgcaatg ctattgtcta tttgattaag
      961 tttttcgtag caaaaggcaa tttcaaataa aatattgatg ttatacacat ttcgagtgtg
     1021 tccaataagt aagaaatcaa gtgctttttg gtaatattca ttatttaaat acacataagc
     1081 aatatccaaa tagattgttt catcttcttg tgcataggta ttaattagct ctttgcagtt
     1141 tttttctgct tgtacttctt gcttggtttt gagcaatatt tcggtttgta gtagatgtaa
     1201 ttctatatca gagggatata aagctactaa tgaattggta tgttgcaaag ctagctctag
     1261 gtcgttcaat tctattaaga tacgtacttt agatgtttct aacccaatat tgtttgggtg
     1321 gagtttgagc ccaaattcaa tagctcgttt agcatccttt acacgcattc ttcgcaaata
     1381 gaaatcagct aattgctcca tttcttctgc atcaaaatag cctccagtaa gattggattc
     1441 ataattttta agaagttgag cgaaccgttc attagcgtta tctaactgct tcatggtatt
     1501 cctttttctg caaaaataca agtaaattgg ctgataacga aacttataca ttaaaagtta
     1561 ttaacaacaa aaaatgtgtt gattgtaatt tattctttca gttttgttgt agattcgtga
     1621 tagcactctc tacataatgg ttgatagtcg gctgtttctc cgagcaacac ttgtttgtcc
     1681 gaatttgtta aacgatacga ataaagggct aaatgaccac atttaacaca aatagctctt
     1741 actttggtaa catgttcagc aatagcacat aaagcaggca ttggaccaaa cggattaccc
     1801 ttaaagtcca tatccaaacc cgctatgatt acacgcattc cttgattggc taattgtcta
     1861 cacacatcta ttaatcccat atcaaaaaat tgagcctcat caatacctat tacatcaacg
     1921 tcatttgaaa gtagtaatat attttgtgaa gagtcaacca ccgttgatag tattg
//
LOCUS       DAOSMY010000289         1967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_57875_length_1967_cov_3.343619, whole genome shotgun sequence.
ACCESSION   DAOSMY010000289 DAOSMY010000000
VERSION     DAOSMY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57875_length_1967_cov_3.343619"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLD76_06270"
     CDS             complement(<1..903)
                     /locus_tag="PLD76_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU27640.1"
                     /translation="MRNFLKLYGFLLFFVLGIIGLNAQSVSINEIMSSNSKTITDEDG
                     SFEDWIELYNYGDTPVNLHQFGLSDNANNRFKWTFPEIMLPPQEFLLVFASDKDRKFV
                     PMEEQVLVSSTTIWKYVDAGEDLGSDWKEIDFDDTEWKTGTAPLGYNTNGNERYATVL
                     DYGSDKSNKYISYQVRTLFEKPTADFRQFTIQLVADDGIIVYLNGKEIIRQNMPDGEI
                     AYTTLAQSGITSIDTINYQLDGSLLQNGTNTIAVSVHQVTLNSSDIYFDMKLWTNKQY
                     PHANFKIASEGETLFLTNKEDSIIS"
     gene            complement(1004..1930)
                     /locus_tag="PLD76_06275"
     CDS             complement(1004..1930)
                     /locus_tag="PLD76_06275"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOU27641.1"
                     /translation="MKIAIIGTGGVGGYFGARLLEAGFDVSFLARGEHLEAIKKNGIQ
                     LKSLLGDIQTKPCKASDKISDLGVSDVLIIGVKAWQIKEIRTELQSIMHKDSIVLPLQ
                     NGVMAADELSETIDKQHIVGGLCRIVSMITAPGCIAHTGVTPTIVFGELDHTISTRLQ
                     NLQENFKKANIHCQLSTNIETEIWKKFLLICISGLMAVTRCTYGEMNEVELTRQMMFK
                     LLEEIYAVGMAKGIQYEPDIVARTVASFDALPYNTTFSLTRDVWEGKPSELEYQNGTV
                     VHIGKQVKVPTPINEFIYTSLLPLEKKARTLK"
BASE COUNT          581 a          391 c          316 g          679 t
ORIGIN      
        1 cgaaataatt gaatcttctt tgtttgtcag aaaaagggtc tctccttcag atgctatttt
       61 aaaattggca tgtggatatt gtttgtttgt ccacaatttc atgtcaaagt aaatatcact
      121 actattcaag gtaacttggt gtacagaaac ggcaattgta tttgttccat tttgtagcag
      181 cgaaccatct agttggtaat tgattgtgtc aatgctagtg atcccacttt gagctaaggt
      241 agtataagca atttctccat cgggcatgtt ctgacggatt atttcctttc catttagata
      301 cacaataata ccatcatccg ccacaagttg tatagtaaac tgtcgaaaat ctgctgtagg
      361 tttttcaaat aatgttctaa cctgatagct tatatatttg ttgcttttat cactcccata
      421 atctaaaact gtagcataac gttcatttcc atttgtatta tatcctaaag gagctgttcc
      481 tgttttccat tcggtatcat caaaatcaat ttctttccaa tcagaaccta aatcttcgcc
      541 agcgtctaca tacttccata tagtggttga cgaaacaagt acttgctctt ccattgggac
      601 aaattttctg tctttatctg atgcaaaaac tagtagaaat tcctgtggag gtagcataat
      661 ttcagggaat gtccatttaa atcggttgtt tgcgttatca gataagccaa attgatgtag
      721 attgacaggt gtatctccat agttgtacag ttcaatccaa tcttcaaaac tgccatcttc
      781 atcggtaatt gtttttgaat tagacgacat tatctcatta atggacacac tttgagcgtt
      841 tagcccaatg ataccaagaa caaaaaacaa taaaaaacca tataatttga ggaaatttct
      901 cattataagt ttgaatacac ttcctattaa ttttgagggt gcaaaggtac aaaaactaaa
      961 tggaaggggc aaaaatcacc ttaaaaattt acctaaaagt ctattattta agggttctgg
     1021 cttttttttc taagggtaaa aggctggtat aaataaattc gttgatagga gtaggtactt
     1081 ttacttgttt tcctatatga acaacagtac cattttggta ttcaagctct gagggttttc
     1141 cttcccatac atctcgtgta agcgaaaacg ttgtattgta gggtaaagca tcgaaagatg
     1201 ccactgttct tgcaacaata tctggttcat attgaatgcc ctttgccatg cctactgcat
     1261 aaatttcttc aagtaactta aacatcattt gtctcgttaa ctcaacctca ttcatttcac
     1321 cataagtaca tcttgtaaca gccattaacc cactaataca aatcagcaaa aactttttcc
     1381 agatttctgt ttcaatattg gttgaaagtt gacaatgaat gttggctttt ttgaaatttt
     1441 cttgcaaatt ttgcaacctc gtcgaaattg tatggtctaa ttctccaaac acaatagtag
     1501 gtgttactcc cgtatgggca atacaaccgg gagctgtaat catacttaca atacggcaaa
     1561 gtccgcctac aatatgctgt ttgtcaatcg tttcactcag ttcgtctgca gccattactc
     1621 cgttttgtag gggcaatacg atgctatctt tgtgcataat gctttgcaat tctgtgcgaa
     1681 tttcttttat ttgccatgct tttacgccaa taattagtac atcagaaacg cctaaatcgc
     1741 tgattttatc agaagctttg catggtttag tctggatatc tcctaataag ctttttagct
     1801 gtatcccatt cttcttaatt gcctccaaat gttcgcctct tgccaaaaac gacacatcaa
     1861 aacctgcctc caacagccga gcaccaaaat atccgcctac gcctcctgta cctattatcg
     1921 ctattttcat ggtaaattta tttaataatt ttctacaatc cctctag
//
LOCUS       DAOSMY010000290         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_57922_length_1966_cov_4.006803, whole genome shotgun sequence.
ACCESSION   DAOSMY010000290 DAOSMY010000000
VERSION     DAOSMY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57922_length_1966_cov_4.006803"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..512
                     /locus_tag="PLD76_06280"
     CDS             33..512
                     /locus_tag="PLD76_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU27642.1"
                     /translation="MLVNHSESVANKALSIAHNHPELKIDTEFVFQAAMLHDIGIHLT
                     NAPSIACYGSYPYVCHGYLGSGILMEEQLPLHALVCERHTGTGISLEDIVTRKLPLPL
                     RDMKPISLEEQLICFADLFFSKTHLEEELSITTIETKLQKFSSHSVVQFHQWCDLFL"
     gene            complement(501..1403)
                     /locus_tag="PLD76_06285"
     CDS             complement(501..1403)
                     /locus_tag="PLD76_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU27643.1"
                     /translation="MNTENITLAFNKIITDVVNWLLYDFTYVLISLVVVIVLIWLLNA
                     ISRNLEKVILKRSDALHDKEAQKRVKTLMSIGRGIMRLILWAIFALILLKRIGIEIGP
                     IIASAGIAGIAIGFGAQELIRDFLAGFFVLLDNQIRTDDIVVINGTTGVVEKIELRTI
                     RLRDHSGVVHIFQHGKINTLSNMTKEWSALVFEIGVAYKEDVDTVIAIMKSVGEDLEK
                     DSRFSGNILEPISILGVEQFANSSIQLKVRIKTQTGEQWQTRREYMRRLKYAFDKKGI
                     EIPFPHTTLYWGEKSKPMDISLQK"
     gene            complement(1409..>1966)
                     /locus_tag="PLD76_06290"
     CDS             complement(1409..>1966)
                     /locus_tag="PLD76_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27644.1"
                     /translation="KFITTMINELKNTYNIDKNKIFVTGFSNGASMTFRLGMELDCIK
                     AIAPVAGVNWIKNNTSNRKISLLYIIGAQDRAKPLEGGITKTANGIVLEKTPKPPIYE
                     NSKRWATFIDCIGEPTTFTLAQGVSGLRFTDCSSNTSIEYIIVDDLGHIWPGARQIIP
                     KSIVGNASEKLNATEYIWNFFNTAK"
BASE COUNT          582 a          367 c          328 g          689 t
ORIGIN      
        1 aatattattc ttcaaatgaa gaagcgtatg acatattggt aaatcatagc gagagtgttg
       61 ctaataaagc cctttctatt gctcacaatc atccagaatt gaagatagat acagaatttg
      121 tttttcaggc agctatgttg cacgatattg ggatacattt aaccaatgcc ccaagtattg
      181 cttgttatgg ttcttatcca tatgtttgtc atggctattt agggtctggc atcttgatgg
      241 aagaacaatt gcctctgcat gccctagttt gcgaacgcca tacaggtaca ggtattagtt
      301 tggaagatat tgtaactcgc aagttgcctc tcccattacg agacatgaaa cctatatcgt
      361 tagaagagca attaatttgt tttgccgatt tatttttttc gaaaacacat ctagaagaag
      421 aattatctat cacaacgatt gaaacgaaat tgcaaaaatt tagttcacat tctgttgtgc
      481 agttccacca gtggtgcgat ttatttttgt agtgaaatat ccatgggttt gcttttttcg
      541 ccccagtaca gtgtggtatg agggaaagga atttcaatac cttttttatc aaatgcatat
      601 tttaatcgac gcatatattc tcttctagtt tgccattgct cccctgtttg ggttttgata
      661 cgcactttga gttgaataga actgttggca aattgttcta ctccaagtat agagataggc
      721 tctaatatat ttcctgaaaa tcttgaatct ttttctaaat cttcacctac tgatttcata
      781 atagctatta ccgtatccac atcttctttg tatgctacac cgatttcaaa tactaatgcc
      841 gaccattctt tggtcatatt agataaggta ttgatttttc catgttgaaa aatatgaact
      901 acaccagaat ggtcgcgtaa acgaattgta cgtaattcaa ttttttccac taccccagtt
      961 gttccattaa ttactacgat gtcgtctgtt ctaatttgat tgtcaagtaa tacgaaaaaa
     1021 cctgctagaa aatcacgaat taattcttga gctccaaaac cgatagcaat acctgcaatg
     1081 cctgcactag cgataattgg acctatttct attccaatac gttttagtaa aattaatgca
     1141 aaaattgccc ataaaatgag gcgcataatg cctctgccaa tgctcattaa agtttttact
     1201 cttttttgag cttctttgtc gtgcaaagca tccgaacgct ttaggataac tttttctaga
     1261 ttgcgagaga tggcattgag tagccaaatc aaaacaatga caacaactaa actaattagt
     1321 acatacgtaa aatcgtacaa gagccaattt acaacatctg ttataatctt gttgaatgct
     1381 aatgttatat tttcagtatt catattcttt attttgcggt attaaaaaaa ttccaaatat
     1441 actccgtggc atttagtttc tcagaagcat tccctactat tgattttggt attatttgtc
     1501 ttgcccctgg ccatatatgc cccaaatcat caacaataat atattcaata gaggtattag
     1561 atgagcagtc ggtaaatcgc aatccgctga ctccttgggc aagagtaaat gttgttggtt
     1621 ccccaataca atcaataaat gtagcccatc gtttactatt ttcatatata gggggcttgg
     1681 gtgttttttc taacacaatg ccatttgccg tttttgtaat acccccttct agtggcttag
     1741 ctctatcttg tgctccgata atatacagga gagaaatttt tctattggat gtattatttt
     1801 tgatccaatt aactccagct accggggcaa ttgcttttat acagtccaat tccattccca
     1861 agcggaaagt cattgatgca ccattagaaa aacctgttac aaaaatcttg tttttgtcaa
     1921 tattatatgt gttttttaat tcgttgatca ttgtggttat aaactt
//
LOCUS       DAOSMY010000291         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_57926_length_1966_cov_3.529042, whole genome shotgun sequence.
ACCESSION   DAOSMY010000291 DAOSMY010000000
VERSION     DAOSMY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57926_length_1966_cov_3.529042"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..>1966
                     /locus_tag="PLD76_06295"
     CDS             198..>1966
                     /locus_tag="PLD76_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU27645.1"
                     /translation="MKSFVHLHVHSYYSLLDGMSSISALVDKAQKDGMPAIALTDHGS
                     MFGIKEFYDYTKKKNKKVKEDIAILEKELLAATDDKQRELWKEKIAATTCKYVKPIIG
                     SEVYVAKRTRFDKTEEIDRSGNHLVLLAKNKKGYRNLCKLVSYGYIEGYYYRPRIDKE
                     LLEKYSEGLIVSSACLGGEIHRLVEKGNIEEAEKSILWFKRVFGDDYYLELQRHETDK
                     PGADTHVFEKQQRQNKVLIELARKTNTKFIATNDVHFVEEEHAEAHDRLICLSTGKDL
                     DDEKRMRYSKQEWLKTTDEMATIFSDLPEAIENTLEIANKVENYSIDEPNPIMPTFDI
                     PIDFGTEEGYRQQYTHQDLLKEFTADEHGVMHLSEKEALEKVHRLGGIDRLYRIKLEA
                     DYLRKITFDGAQVRYGENIPQEVKERIEFELHVMKTMGFPGYFLIVYDFIQAARKMEV
                     TVGPGRGSAAGSVVAYCLRITDIDPLKYDLLFERFLNPDRISMPDIDIDFDDDGRSKV
                     LDYVAHKYGKERVAQIITYGTMATKMAIKDVARVQKLSLPESERLTKLIPTSFPEENG
                     KSPKVTISNCIERIPELREARYSS"
BASE COUNT          685 a          279 c          403 g          599 t
ORIGIN      
        1 aaggtacatg ctttagaata tgaacatttt ccgcatgtat taaaaatgtt atggtcataa
       61 attttcacta tatcttttga cacttaaaca gttatgggta gacctagctt tttgtttaaa
      121 actttataga attttatttt ttctacttta agagaatcac taactttgga tttctgaaat
      181 aatttacctt tttatatatg aaatcctttg tacatttaca cgttcactct tattattctt
      241 tattggatgg tatgtcttct attagtgcct tggttgataa ggctcaaaaa gatggcatgc
      301 ctgctattgc tcttacagac cacggttcaa tgtttggcat aaaagagttt tatgattata
      361 ccaaaaagaa aaataaaaaa gtaaaggaag atattgctat attagaaaag gaattactag
      421 ctgcaacaga cgacaaacaa cgagaattgt ggaaagaaaa aattgcagca actacttgta
      481 agtatgtcaa accaattata ggaagcgaag tgtatgtagc taagcgtact cggtttgata
      541 aaacagaaga aatagacaga agtggaaatc atcttgtatt gttagcaaaa aataaaaagg
      601 gctatcgaaa tctatgtaaa ttagtttcgt atggatatat cgagggttat tattatcgtc
      661 ctcgcattga caaagaactg ctagaaaaat acagcgaagg cttgattgtg tcttcggctt
      721 gtttgggggg tgaaattcac cgtcttgtag aaaaagggaa tatagaggaa gcagaaaaaa
      781 gtattttgtg gtttaaacgt gtttttggtg atgattatta tttggaattg caacgacatg
      841 aaacggacaa accaggagca gatacacatg tatttgaaaa gcaacaaaga cagaataaag
      901 tattaataga acttgcacgt aaaacgaata cgaaatttat tgcaaccaat gatgttcatt
      961 ttgtagaaga agaacatgca gaggctcatg atagacttat atgcttgagt actggtaaag
     1021 atttggatga tgaaaagcgt atgcggtata gtaaacaaga gtggctaaag actacggatg
     1081 agatggctac tatattttca gatttacccg aagccataga aaatacgctt gaaattgcaa
     1141 acaaggttga aaattatagc attgacgaac ctaatccaat aatgcctact tttgatattc
     1201 caatagattt tggaacagaa gaggggtatc gccaacaata tacacatcaa gatttgctga
     1261 aagagtttac tgcagatgaa catggtgtta tgcatctttc tgaaaaagag gctttggaaa
     1321 aagtacatag attgggaggt attgataggt tgtatcgcat taaactagaa gcagattatt
     1381 tgcgaaaaat tacatttgat ggagcacaag tacgatacgg agaaaatatt ccacaagaag
     1441 tgaaagaacg tatcgaattc gaacttcatg taatgaaaac aatgggtttt ccaggatatt
     1501 ttctaattgt gtacgacttt atccaagctg ctcgtaagat ggaagttaca gtaggtccag
     1561 gaaggggttc ggctgcaggt tccgtagtgg catattgttt gcgaattaca gatatagatc
     1621 ctttaaaata tgatttgctg tttgaacgtt ttctaaatcc tgatcgtatc tcgatgcctg
     1681 atattgatat tgattttgat gatgatggca gatcaaaagt gcttgattat gtcgctcata
     1741 aatatggcaa agagcgtgtg gctcagatta ttacttatgg aacgatggcg actaaaatgg
     1801 ctatcaaaga tgtggcacgc gtgcaaaaat tatctcttcc cgaatcagaa agattaacta
     1861 agttgattcc aactagtttt ccagaagaaa atggcaaatc gcctaaagta actatctcta
     1921 attgtataga acgaattcct gaattacgtg aagcacgtta ttcttc
//
LOCUS       DAOSMY010000292         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_58114_length_1962_cov_2.859990, whole genome shotgun sequence.
ACCESSION   DAOSMY010000292 DAOSMY010000000
VERSION     DAOSMY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58114_length_1962_cov_2.859990"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLD76_06300"
     CDS             complement(<1..603)
                     /locus_tag="PLD76_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOU27646.1"
                     /translation="MTKERIQKILKKRSVFSHKGTFGHCLLMNGSYGKMGAGILSAKA
                     CLRSGVGLLTTHIPVCGNTIMQIAVPEAMTYTDMQELTLTSVHDNINDFDAVGCGCGI
                     GTAIHTQKMIHNLLLSTNTPFVLDADALNCIALNNWKHLLPKNTIITPHPKEFDRLVG
                     ESKHNGERLQKAILLSKQLTIVIILKGAYTRVVLPNGKVYF"
     gene            complement(667..1272)
                     /locus_tag="PLD76_06305"
     CDS             complement(667..1272)
                     /locus_tag="PLD76_06305"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOU27647.1"
                     /translation="MKIFNSTAIRQIDAYTIEKNHIKSIELMEVAARSVFEYIIQHYD
                     IEKKIIIFAGQGNNGGDALALARLLHIAGFSIKVITVFLSDNLSDDCETNYKALLNKN
                     ISICHYSRYLELNIEEDYLIIDGIFGTGLNKPITGKLADMIVYINSRNCEIIAIDIPS
                     GLPSDGVTETNPIAIKATITISFQFPKISFFFAEYESFIGK"
     gene            complement(1328..1960)
                     /locus_tag="PLD76_06310"
     CDS             complement(1328..1960)
                     /locus_tag="PLD76_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU27648.1"
                     /translation="MNVKTFIFNPFQENTYVLSDSNNKCIIIDAGCLNLEEKNDLYSY
                     IKENNLLVERILNTHLHIDHCFGNQFVSETFNKKIEAHPDDEALLNGMNKYAQTFGIE
                     YTENHPTISYIFEGDTITVGEISLKAIHVPGHSKGSLVFYAEKEKYLFAGDVLFSGSI
                     GRTDLPGGNFHDLIDNIKQKLLILPDETVVFSGHGAQTTILHEKQYNPFL"
BASE COUNT          624 a          312 c          327 g          699 t
ORIGIN      
        1 aaaatacact tttccattgg gaagaacgac tctggtatat gcacctttca gtatgataac
       61 aatagttaat tgtttgctta gaaggatggc tttttgtaat ctttcaccat tatgcttgct
      121 ttcgcctacc agtctatcaa attccttagg gtgaggagtg atgattgtat ttttaggtaa
      181 taaatgtttc cagttattta gtgcaataca gttaagagca tcggcatcta aaacaaaagg
      241 ggtattggta gataaaagta agttatgtat cattttttga gtatgtattg cagtaccaat
      301 accacaacca caacctactg catcaaaatc gttgatgtta tcatgtacac tagtcaatgt
      361 aagttcttgc atatctgtat atgtcattgc ttctggtaca gctatttgca ttatggtgtt
      421 tccacaaaca ggaatatgtg ttgtgagcaa gcctactcca gatcgtaaac aagccttagc
      481 tgataagatg ccagcaccca tttttccata acttccattc attaataggc agtgcccaaa
      541 agtgcctttg tgagaaaata cagagcgttt ttttagaatc ttctgaattc tctctttcgt
      601 aattgtatag aaatttgtct ctttattagc agtatattct ttagcaagtc caatattagt
      661 aagtatctat tttcctataa atgattcgta ctcggcaaag aaaaaagata tctttggaaa
      721 ttgaaaacta atggtaatag tagctttaat agcaatagga ttagtttcag taactccatc
      781 agagggtagt ccagaaggga tatcaatggc tataatttcg caattacgtg aattgatata
      841 cacaatcatg tcagcaagtt taccagttat tggtttgttt agtccagtgc caaaaatgcc
      901 atcaataatc aagtaatctt cttctatgtt tagttctaag tatctgctat aatggcagat
      961 agatatattt ttattaagta gtgctttata gtttgtttca caatcatctg ataaattatc
     1021 cgataaaaac acagtaatta ctttgattga aaagcctgct atgtgtaaca aacgagctag
     1081 ggctaaagca tctccaccat tgttgccttg tcctgcaaaa atgattattt ttttttctat
     1141 atcataatgt tgtataatat attcaaatac agaacgagca gctacctcca ttaattcaat
     1201 tgatttaatg tggttttttt caatggtata agcgtctatt tggcgaatag ctgtagaatt
     1261 gaatattttc ataccattgg aaattagatt tggaggtaca tagtatgagg agatgatatt
     1321 ttacgtttta taaaaaaggg ttgtattgtt tttcgtgcaa tatggttgtt tgagccccat
     1381 gaccagaaaa aacaactgtt tcatcgggca aaataagtaa tttttgctta atattatcaa
     1441 ttaaatcgtg aaagtttcct ccaggaagat ctgttcttcc gatggaacca ctaaataata
     1501 catcacctgc aaaaagatat ttttcttttt ctgcataaaa gactaagctc ccttttgaat
     1561 gccctggcac atgtattgct tttaatgaaa tttcacctac tgtaatggta tctccttcaa
     1621 aaatataact gatagtaggg tgattttcag tatattcaat tccgaacgtc tgagcatatt
     1681 tattcatacc atttaataga gcttcatcat cgggatgagc ttcaattttt ttattaaatg
     1741 tttctgaaac aaattgatta ccaaagcaat ggtcaatatg tagatgggta tttaaaatac
     1801 gttcaacgag taaattattt tctttaatgt aggaatacaa atcatttttt tcttccaaat
     1861 ttaggcaacc tgcatcaata ataatgcact tattgttgct gtcgctcaat acataggtat
     1921 tttcttgaaa agggttaaaa ataaatgtct taacgttcat tt
//
LOCUS       DAOSMY010000293         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_58143_length_1961_cov_3.490556, whole genome shotgun sequence.
ACCESSION   DAOSMY010000293 DAOSMY010000000
VERSION     DAOSMY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58143_length_1961_cov_3.490556"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1961)
                     /gene="dnaE"
                     /locus_tag="PLD76_06315"
     CDS             complement(<1..>1961)
                     /gene="dnaE"
                     /locus_tag="PLD76_06315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU27649.1"
                     /translation="RIPELHEARYSSDARLSDTLKYAEMLEGTVRQVGIHACGVIIGA
                     DDLTNFVPISIDKNNSETLVTQYEGSTIETVGLIKMDFLGLKTLSIINETLRNIKASK
                     GITIDIDTIPIDDKKTYELYSKGLTTGTFQFESSGMQKYLRELKPNRFEDLIAMNALF
                     RPGPMAYIPSFINRKHGIEEIQYDIPIMEKRLKETYGITVYQEQVMLLSRDLAGFTRG
                     ESDELRKAMGKKLQDKMDALKVKFINGATKNGYGPIELLEKIWKDWAEFAKYAFNKSH
                     ATCYSWISYQTAYLKANYTADFMAANLTRNKDNITEITKFMEECKQLGIKVLGPSVNE
                     SNLNFISNPAGDIRFGLGGIKGVGYGAVEAIVTERQANGRYTSVFNFLERVNLNACNK
                     RAIESLALSGAFDEFDDVKREAFFAENSKGEVVLETLMRYASRYQLDQQSSQQSLFGD
                     LESVAIQRPEIPQVQSWSNLYKLTKEKDMIGIYLSAHPLDFYEIELASLCTQDVTRLK
                     ELSELHDSIGSNPVKIAGIITKVNRLISQNNQPFARFTLEDYSGSHEISVFGKQFTEL
                     DNMLRENYYVLIHGVVQERQADYKFKPKPEDEARGRELVFKIQTIELLETIKDKGIEE
                     LKIQIPLLNISYELIAELSALLSENVDKK"
BASE COUNT          598 a          393 c          275 g          695 t
ORIGIN      
        1 cttttttgtc tacattttct gacaataaag cagataattc ggcaattaat tcatacgaaa
       61 tatttaaaag gggaatctgt atttttaatt cttctatacc tttatcttta attgtttcca
      121 ataattcaat tgtttgaatt ttaaacacca attcacgtcc acgtgcttca tcttctggtt
      181 taggtttaaa cttatagtct gcttgtcgtt cttgtaccac tccatgtatc aatacataat
      241 aattttctct caacatattg tctagctcgg taaattgttt gccaaataca ctaatttcat
      301 gcgatcctga ataatcttcg agtgtaaagc gagcaaaggg ttgattattt tgagaaatta
      361 atcggttaac tttggttata atacctgcta ttttaacagg atttgaacct atactatcat
      421 gcaattctga taattctttt aatcgtgtaa catcttgtgt acacaaggat gcaagttcaa
      481 tttcgtaaaa atctagagga tgagccgaca agtaaatccc aatcatgtct ttttccttag
      541 ttagtttata taaatttgac cacgattgta cttgaggaat ttcaggtcgt tgaatagcaa
      601 cagactctaa atctccaaac aacgattgtt gagaagattg ctgatcaagt tggtatctac
      661 tagcataacg cataagggtt tctaaaacaa cctctccttt agaattttct gcaaagaaag
      721 cttctctttt tacatcatca aattcatcaa atgctcctga taaagccaaa ctttctatcg
      781 ctcttttgtt acaagcattt agattcaccc tttctaaaaa attaaaaaca cttgtgtacc
      841 ttccatttgc ttgcctttca gttacaatag cttctacagc accatacccc actcctttta
      901 tgccaccaag tccaaaacga atatcgccag ctggattaga tatgaagttt aaattacttt
      961 cgtttacact tggacctaaa actttaatac caagctgctt acactcttcc ataaacttgg
     1021 taatttcagt aatattatcc ttgttcctcg taaggttcgc cgccataaaa tcagccgtat
     1081 agttagcctt taaataagca gtctgataag atatccacga ataacaagta gcatgtgatt
     1141 tattaaaagc atactttgca aattcagccc aatctttcca aattttttcc aacaattcta
     1201 ttggaccata gccatttttg gttgcaccat tgataaattt aacttttaag gcatccattt
     1261 tatcctgcaa ctttttcccc atagcttttc gcaattcatc agactcacca cgtgtaaatc
     1321 cagctaaatc tcgactaagt aacatcacct gttcttgata cacagtaatc ccatacgttt
     1381 cttttaatcg tttttccatg atgggtatat catactgtat ttcctctatt ccatgttttc
     1441 tgttaataaa gcttggaatg taagccatcg gacctggtcg aaacaaagca ttcatggcta
     1501 ttaaatcctc aaaacggttg ggttttagtt cacgcaaata tttttgcatt ccgctcgatt
     1561 caaactgaaa tgtaccggtg gtcaatcctt tgctatacaa ctcataggtt tttttatcat
     1621 ctatgggtat agtatcaata tctatagtga taccttttga tgctttgata ttacgcaacg
     1681 tttcgttaat aatagacaag gttttcaacc ctaaaaagtc catttttatt aaaccaaccg
     1741 tttcaatagt tgatccttca tattgtgtaa ctaaagtttc ggaattattt ttatcaatac
     1801 taataggaac aaaattggtt aaatcatcag caccaataat aacaccacat gcatgtatgc
     1861 ctacttgtct tactgttcct tcaagcattt cggcatattt taaagtatcc gataagcgag
     1921 catctgaaga ataacgtgct tcatgtaatt caggaattcg t
//
LOCUS       DAOSMY010000294         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_58466_length_1953_cov_3.562698, whole genome shotgun sequence.
ACCESSION   DAOSMY010000294 DAOSMY010000000
VERSION     DAOSMY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58466_length_1953_cov_3.562698"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="PLD76_06320"
     CDS             <1..1658
                     /locus_tag="PLD76_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOU27650.1"
                     /translation="MLLRADMYEVNVKFLDPYLSSFAKDIEGEATGVIYIGGDLRAIE
                     VWGKAFINKAKLHIDYLKTDVYFSDYITLTKNSILLSDITIADKKGNKGTVNGKITHK
                     AFSEFLFNIDLLLKNMLVYNTTENDNPDFYGTVYASGIANIYGNVDLLSIDVAGKTEK
                     NTVFYIPINTSTSALEGDFITFVNRKQLSTTTRERRRTQQSESTESKLRISVQIEATP
                     DAEAILILDDRTREYIQGNGSGNIRLSLEPNENLKMYGNYTLNDGKYFFVMQNAIRKT
                     FEIKDGSSIAWDGNPYNGAIDIMATNQVSASLIDLLDASMLQDVKRLTVPVLCKAHLT
                     GELQQPTIKLDLELPTADDELKRRVQNIVNSDEMMSNQMVFLLMLGRFYNPQLLTNTP
                     GMATMIASATLSSQLNYWLSQISNDVNLGVNYRQSEGGDITSREIEVALTTNLLDNRL
                     IINSNVGYKEDAFTETNFIGDFDIEYKINRSGRLRLKAYSHSNDKYYVRNALTTQGVG
                     VIYREDFASRKTLWNYYRRLFKLKAKNKTSQEGIVEEPNENIK"
     gene            1661..>1953
                     /locus_tag="PLD76_06325"
     CDS             1661..>1953
                     /locus_tag="PLD76_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU27651.1"
                     /translation="MITQTIGGNSHLQSSRIIMGCMRIDSLSQHDLQYLIDTAINNGI
                     SYFDHADIYANGYCEEHFGNSFRGNRENIVLQTKCGIKSGVFDFSKQHIISSV"
BASE COUNT          717 a          317 c          347 g          572 t
ORIGIN      
        1 atatgctttt acgtgctgat atgtatgaag tgaatgtgaa atttttagat ccctacttat
       61 cttcatttgc caaagatatt gaaggagaag ctacgggtgt aatatacatt ggtggcgacc
      121 tacgagcaat agaagtatgg ggaaaagctt tcataaataa agctaaatta catattgatt
      181 atcttaaaac agatgtatat ttttctgact atataacctt aacaaaaaat tctattttgt
      241 tatctgacat aaccattgcg gataaaaaag ggaacaaagg taccgttaac ggaaaaatta
      301 cacataaagc attttctgag tttttattta acattgattt attgctcaaa aatatgttgg
      361 tatacaacac aacagagaat gataatcctg atttttatgg aacagtatac gcatcaggta
      421 tagcaaatat atatgggaat gtggatttac tatcaattga tgtagcgggc aaaacagaaa
      481 aaaatacggt cttttatatt ccaatcaaca caagtactag tgcactagaa ggagacttta
      541 taacctttgt caacagaaaa caactttcta caacaacaag agagagaaga cgtacccaac
      601 aatcagaatc aactgaatca aaactacgca tatcagtaca aattgaagcc acaccagatg
      661 ctgaagcaat tctaatttta gatgatagaa cacgagaata cattcaaggt aatggcagtg
      721 gcaacattcg actaagctta gagccaaacg aaaacttaaa aatgtatggt aattacaccc
      781 taaatgacgg aaaatatttt tttgttatgc aaaatgctat cagaaaaaca tttgaaatca
      841 aagacggaag tagtatagct tgggacggaa atccgtataa tggagccatc gacataatgg
      901 cgaccaatca agtatctgca tctcttattg atttattaga tgccagtatg ttgcaagatg
      961 taaaaagact caccgttcct gttttatgta aagctcattt aacaggagaa ttacagcaac
     1021 ctactattaa attagattta gaactaccta ctgctgacga tgaattaaaa cgaagagtgc
     1081 agaatatagt aaactctgac gaaatgatga gcaatcaaat ggtcttttta ttgatgttag
     1141 ggcgtttcta taatcctcag ctacttacta acacaccagg gatggcaact atgatagcct
     1201 ctgccacact ttcaagccaa ttgaattatt ggttatctca aattagtaac gacgtaaacc
     1261 ttggtgtaaa ttatcgtcaa agtgaaggag gagacattac tagtagagaa atagaagtag
     1321 ctttaacgac taatttactt gataataggc ttataataaa tagcaacgtg ggatacaaag
     1381 aagacgcctt tactgaaact aattttattg gcgattttga catcgaatat aaaatcaatc
     1441 gttctggcag gttacgcctc aaagcttact ctcactctaa cgataaatat tatgtgcgta
     1501 atgctcttac tacacaaggt gttggtgtca tataccgcga agacttcgct tcacggaaaa
     1561 ctctttggaa ttactatcga agattattta aactcaaagc gaaaaacaag actagccaag
     1621 aaggaatagt tgaagaacct aatgaaaata taaaataaac atgataacac aaacaattgg
     1681 tggtaattca catctgcaat catctcgtat tattatgggt tgtatgcgta tcgactcctt
     1741 atctcaacac gatttgcaat accttattga tacggctatt aacaatggca ttagctattt
     1801 cgatcatgct gatatatatg ccaatggtta ttgcgaagaa cattttggaa atagttttcg
     1861 tggcaataga gaaaatattg ttttacaaac aaaatgtggt ataaaatcgg gtgtattcga
     1921 tttttccaaa cagcatatta ttagcagtgt aga
//
LOCUS       DAOSMY010000295         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_58653_length_1949_cov_2.733369, whole genome shotgun sequence.
ACCESSION   DAOSMY010000295 DAOSMY010000000
VERSION     DAOSMY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58653_length_1949_cov_2.733369"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLD76_06330"
     CDS             complement(<1..246)
                     /locus_tag="PLD76_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27652.1"
                     /translation="MLYKRTNGIGFVAAKKRFAMEIEDIITTMAIIYCGGDIGKYGEV
                     DFRRHIPKETFTMVNNGIYLFIFRTLLITNHTRRGVVG"
     gene            complement(431..697)
                     /locus_tag="PLD76_06335"
     CDS             complement(431..697)
                     /locus_tag="PLD76_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOU27653.1"
                     /translation="MKTYKFCQSCGYPLKKDTQGGGSEKDGEISKKYCSMCYDNGKFI
                     TPAEIDTAKKMKTFCIQEMKNTGINGFLAWLATRSIPTLERWKT"
     gene            complement(803..1675)
                     /locus_tag="PLD76_06340"
     CDS             complement(803..1675)
                     /locus_tag="PLD76_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOU27654.1"
                     /translation="MKKRYESSELIVNADKSIFHLHLHPEQIADKIILVGDLGRVDVI
                     SGYFDTIESEAQNREFKTVTGTYKGKQITVVATGIGTDNIDIVVNELDALANIDLDKR
                     EDKDAFRKLTFVRIGTCGGLQKNIPVDSFLVSEQSIGFDGVLNYYANRDSVCDLDFEQ
                     AFIQQTGYSSKLTAPYCIHSTPSLVERIAQNDMLRGVTISAPGFYGPQGRELRLPLAF
                     PDLNQRIEDFEYKGKKITNYEMESSAIAGLATLMGHEAMTVCVVIANRMLKQYSKDYH
                     PAVKKLVELVLERI"
     gene            complement(1798..>1949)
                     /locus_tag="PLD76_06345"
     CDS             complement(1798..>1949)
                     /locus_tag="PLD76_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HOU27655.1"
                     /translation="AKVIEAEAEVPKAMAQAFISGNLGIMDYYKMKNIEANTSMRENI
                     AKPKK"
BASE COUNT          567 a          393 c          315 g          674 t
ORIGIN      
        1 gcctacgacg cctcgtcggg tgtgatttgt aatcaggagt gttcggaaga taaataagta
       61 tatgccattg ttaaccatcg taaatgtctc ttttgggata tgtcttctaa aatcgacttc
      121 tccatatttt ccaatatctc caccgcaata gattattgcc attgtagtaa ttatatcttc
      181 aatttccatt gcaaatcttt tttttgcagc cacaaaaccg attccattgg tacgtttgta
      241 aagcattacc tgcaaactat tttcattgac agacagaaaa tgtttaaagt taagttcctg
      301 tttaataaag cgaaagaaaa cttcgatgtc ccatcccctt gttcggcgta gcctaatcag
      361 cgaggctaca actcagcaaa cgtgtgtcaa aaaaaagtat gtgagctgta attgtttatg
      421 tgcacgagca tcaggttttc catctttcca atgtaggaat gctacgagta gctagccatg
      481 caagaaaacc atttatgcca gtatttttca tttcttgtat gcaaaatgtt ttcatttttt
      541 tagcagtatc aatctcggca ggggttataa attttccatt atcataacac atggagcaat
      601 atttcttact tatttctccg tctttctccg aaccaccacc ttgggtgtct ttctttaacg
      661 gataaccaca actttggcaa aatttatacg ttttcatcac agtttattga attacttata
      721 ctaacttcct tcgataacag aataaataca cacatgaacc caatcattat tacgtactta
      781 acagcacaca gtttatgaaa tcctatatcc gttccaacac caattctacc aattttttta
      841 cagcaggatg ataatcttta ctgtattgtt tgagcattct gttggcaatt actacacaca
      901 cagtcatggc ttcgtgtccc atcaatgtgg ctaagcccgc aatagcagaa ctttccattt
      961 cgtaattggt aatttttttg cctttatact caaaatcttc aatgcgttgg tttaaatcag
     1021 gaaaagccaa tggtaaacga agttctcttc cttgtggtcc gtaaaaacca ggagcagaaa
     1081 tagttacccc acgtagcatg tcgttttgtg caatacgctc taccaaactt ggcgtagaat
     1141 gaatgcagta gggtgcagtt agcttactcg aatagcccgt ttgctgaata aatgcttgtt
     1201 caaaatctaa gtcgcataca ctatctcggt ttgcataata attaaggact ccatcaaagc
     1261 caatagattg ttcagacact aaaaaactat ctacaggtat gtttttttgc agaccgccgc
     1321 aagtaccaat acgaacaaat gtaagtttgc gaaaagcatc tttgtcttct cttttatcta
     1381 aatcgatatt agccaaagca tctaattcat ttactactat atcaatgttg tcggtaccaa
     1441 ttcctgtggc tactaccgtt atttgttttc ctttataggt gccggtaacg gttttaaatt
     1501 cacggttttg tgcttcgctt tctatggtgt caaaatagcc acttatcaca tctacacgtc
     1561 ccaaatctcc tacgagaatt attttgtcag caatttgttc tggatgcaaa tgtaagtgga
     1621 agatgctttt gtctgcattt acaattaatt ctgaactttc atatcttttt ttcatagttg
     1681 gtgcttttat tgattagata gtaaataaat actcacttat ttttcgatca ctgatttact
     1741 tctagtgcca aaaacaagtg agtatgtaac tctaagaaaa gaaataagta cgaatgttta
     1801 tttctttggt ttagctatgt tttcgcgcat agaagtatta gcttcgatgt ttttcatttt
     1861 ataataatcc ataataccca agttgccact aataaacgct tgagccatag cttttggcac
     1921 ttctgcctct gcttcaatta ctttggcac
//
LOCUS       DAOSMY010000296         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_58860_length_1944_cov_2.536263, whole genome shotgun sequence.
ACCESSION   DAOSMY010000296 DAOSMY010000000
VERSION     DAOSMY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58860_length_1944_cov_2.536263"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLD76_06350"
     CDS             complement(<1..760)
                     /locus_tag="PLD76_06350"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU27656.1"
                     /translation="MKEMNCLVTGSTGLVGAHLLAHLAQSHLYTVRALKRKTSKMDTL
                     RYVFGFYSQDIDSLLDTITWIEGDILDKDSLAIAMEGIQVVFHCAADVVIGEDITGSV
                     LKTNVQGTQNIVDVALQKKIQILCYVSSVAAIGKSITDSYPDENALWDSNQPHSVYAQ
                     SKYQTEQVVFAIPRNQMRVIVVNPGVILGVNNNKHGSSAVIYLAKKGLPFYTQGGSGY
                     VDVRDVCKAMLLLVQSSVTHERFILVGENCTTKQL"
     gene            848..1567
                     /locus_tag="PLD76_06355"
     CDS             848..1567
                     /locus_tag="PLD76_06355"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOU27657.1"
                     /translation="MHHFYCPDIHSSLQLTEEESAHAIRVLRLGIGDEIEVFDGNGHI
                     CTAIIEQAHPKHTSVRITQTSHIPKNRDYTIAIAIAPTKNMDRLEWFVEKACEIGIDR
                     ITPIICHYSERKICKTDRLHKIIISAIKQSHQAYMPILDETMTFDEWLSTVHAEQKCI
                     AHCYKDATKIELTKKDVINKNISLAIGPEGDFSEKEIKRALQKGFTSVSLGENRLRTE
                     TAGIVACHTIHVLNQLVKKTT"
     gene            complement(1569..1763)
                     /locus_tag="PLD76_06360"
     CDS             complement(1569..1763)
                     /locus_tag="PLD76_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27658.1"
                     /translation="MVIAKRFFLLIVAYQVASASAFAQLMPDTSADKDALSSWELWLT
                     VFVAVLVLGVIQYVKYKNKD"
BASE COUNT          690 a          367 c          286 g          601 t
ORIGIN      
        1 gcaattgttt tgttgtgcaa ttttctccta ctaaaataaa ccgttcatgc gtaacactac
       61 tttgcactaa caatagcata gctttacata catcacgcac atctacatat ccactaccac
      121 cttgcgtata aaaaggtaat ccttttttgg ctaaataaat tactgcacta ctaccatgtt
      181 tattattatt tacaccaaga ataactcccg gattcacaac aataacacgc atttgattac
      241 ggggtatggc aaacactact tgttctgttt gatatttact ttgagcatat acagaatgtg
      301 gttgattact atcccataaa gcattctcat ctggataact atccgttatc gatttgccaa
      361 tagcagccac agaactgaca taacatagaa tttgtatttt cttttgcaat gctacatcta
      421 ctatattttg agttccctgc acattggttt tcaataccga acctgtaata tcttcaccaa
      481 tcactacatc tgcagcacaa tgaaatacga cttgaatacc ctccattgca attgccaaac
      541 tatccttatc taatatatct ccttctatcc aagtaatggt atccaataat gagtctatat
      601 cttgagaata aaacccaaaa acataacgta atgtgtccat tttactggtt ttacgtttta
      661 atgctcgcac agtatataag tgcgattgtg ccaaatgagc caacaaatgt gctcctacta
      721 atccagtact accagtaacc aaacaattca tctctttcat atttgtgcaa agataattaa
      781 aaaggaataa caatacttgt caagttgctt tcaaaatgct atttttgcac ctaaaataag
      841 aaaaactatg catcattttt attgtcctga tattcattcg tcattacaac ttaccgaaga
      901 agaatctgct catgctattc gtgtattacg gttgggtatt ggcgatgaaa tagaagtatt
      961 cgatggtaat ggtcatattt gtacagccat tatcgaacag gctcatccaa aacatacttc
     1021 tgtgcgtatc acccagacct cacatatacc aaagaataga gattatacta ttgcaattgc
     1081 catagctcct acaaaaaata tggataggtt agaatggttt gtagaaaaag catgcgaaat
     1141 aggcatagat agaattacac ccataatttg ccactattcg gaacgtaaaa tatgcaaaac
     1201 agatagattg cataaaatta ttatttcggc aatcaagcaa tctcaccaag cttatatgcc
     1261 aattttagat gaaacaatga catttgatga atggctatca actgtacacg ccgaacaaaa
     1321 atgtattgct cattgctaca aagatgctac aaaaatagaa ttgacaaaaa aagacgttat
     1381 caataaaaac atttcactag caataggacc agaaggagat tttagtgaaa aagaaataaa
     1441 aagggcttta cagaaaggct ttacaagtgt ttcattaggt gaaaacagac tacgcacaga
     1501 aactgctgga attgtggcat gccatactat tcatgtttta aatcagctag ttaaaaaaac
     1561 gacctaaatc aatctttatt tttatattta acgtactgaa taacacctaa aactaacaca
     1621 gctacaaaga ctgttagcca taattcccaa cttgacaatg catctttatc ggctgatgta
     1681 tctggcatta actgagcaaa tgcagaagca cttgcaacct gataagctac gataagcaga
     1741 aaaaatcttt tagcaataac catatttaca ctattagcat ttagtggtac attttttata
     1801 ttgctacaaa gataaaaatt ttatatctca aacatcgaag ttttatgttg tttttatctt
     1861 atttaacatt gaaaaggaat gaagaaatag agattctgca agaaagacta ttttacaaag
     1921 actatggctt gtgtttgctg agta
//
LOCUS       DAOSMY010000297         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_58894_length_1943_cov_3.390890, whole genome shotgun sequence.
ACCESSION   DAOSMY010000297 DAOSMY010000000
VERSION     DAOSMY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58894_length_1943_cov_3.390890"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLD76_06365"
     CDS             complement(<1..789)
                     /locus_tag="PLD76_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOU27659.1"
                     /translation="MNIPTHFSPTKRYLDYSQYMLAFFPERIQKISIHAGFTCPNRDG
                     SKGYGGCTFCNNESFSPAYCSSHTSISQQIEEGISFFGKKHAHQKYLAYFQSYTNSYG
                     SMEVIKQKYSEAIHHRAISGIVIGTRPDCMSDELLDYLHEIQKNTFVLVEYGVESTNN
                     NTLELINRGHTYEESVDAIMRTHAKGIPVAAHIILGLPSENRETILNHATQLATLPLA
                     IIKLHQLQIIKNTPLAKQYTDNPSFIPLYSLQEYIDLCIDFMEHI"
     gene            979..>1943
                     /locus_tag="PLD76_06370"
     CDS             979..>1943
                     /locus_tag="PLD76_06370"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOU27660.1"
                     /translation="MYSEDDLIWLAENGITPESLEKQLQIFTKGVEPPAIKRIATCND
                     GIRVVNDAEVEMYQTAWNDYIENNPDKTTHFIPASGTANRLFRALYRFYYSEHTEPQT
                     NIEKNFFKHLQSFAFFNELNVICIQKEKKDIHELIEQKQYKLILEYMFFEKGANYSQL
                     PNALFKFHTDKSMRYAAFQKYLPKKIAKYASFEHTRTPIQENLLECAMVSGVKKGNVR
                     VCFTVNKEHLNQIAEYIEEYKKPIEKKLSVSLDVHLSVQHPSTQTVIVKDDNSFFRDK
                     DNKITFTYAGHGALLENLNAIDAEIVFIKNIDNAVPDTLKKTTSS"
BASE COUNT          659 a          308 c          345 g          631 t
ORIGIN      
        1 tatatgttcc ataaaatcga tacacaaatc aatatattcc tgtaaactgt acaacggaat
       61 aaacgatgga ttatctgtgt attgttttgc taaaggtgta ttttttataa tttgaagttg
      121 atgcaatttg atgatagcca gcggaagtgt ggctaattgt gtggcatgat ttaatatcgt
      181 ttctctgttt tcagatggca atcccaaaat aatatgtgct gccacaggaa tgcctttggc
      241 gtgtgttcgc atgatggcat caacactttc ttcgtacgta tgtcctcgat ttatcagttc
      301 aagcgtgttg ttattggtac tttctacacc atattcaact agtacaaaag tatttttttg
      361 tatttcatgc aggtaatcaa gcaattcgtc cgacatacag tcgggtcgtg tgccaattac
      421 aatgcccgaa atagctcgat ggtgtatggc ttcgctgtat ttttgtttaa taacttccat
      481 acttccgtac gaatttgtat acgattgaaa gtaggcaaga tatttttgat gtgcatgctt
      541 tttgccaaaa aaagaaatac cttcctctat ttgttgtgaa atggatgtgt gagagctaca
      601 atatgctgga ctaaacgatt cattgttgca aaacgtacaa cctccatatc ctttgcttcc
      661 atctctattg gggcatgtaa atcccgcatg aatagatatt ttctggattc gttctggaaa
      721 aaacgcaagc atatactgtg aataatcaag atatcgtttt gtgggcgaaa aatgggtagg
      781 aatattcatg ctttattatt tttatagtgc tgcatagttg gcttaataca atatccttgc
      841 atgcaaaaaa gctatgtgca aaggtacgat acaaaattga agtattgaaa tagaacgaat
      901 aaaaatcaat acgtaatatt ttttgaagta tcactattaa gttgtatttt tgctgttctt
      961 aattaaaaga aagataggat gtatagtgaa gatgatttaa tttggcttgc agaaaatggt
     1021 attacgcccg aatcgttaga aaaacagtta caaatcttta ccaagggagt tgaaccgcca
     1081 gctattaaac gtatagcaac atgcaacgat ggtattcgtg ttgtaaatga tgctgaggta
     1141 gaaatgtatc aaacagcatg gaatgattat attgaaaata acccagataa aaccactcat
     1201 tttataccag catcaggcac agctaatcgt ctttttagag cattgtaccg tttttattat
     1261 tcagaacata cagaacctca aacaaatatt gaaaagaatt tttttaaaca tttgcaaagt
     1321 tttgcctttt tcaatgagct aaatgtgatt tgcattcaaa aagaaaaaaa agatatacac
     1381 gagttaattg aacaaaagca gtataagctc attctcgaat atatgttttt tgaaaaaggg
     1441 gcgaactata gtcaactccc caatgcattg tttaagtttc atacggacaa atcaatgcga
     1501 tacgctgctt ttcaaaaata tttaccgaaa aaaattgcca aatacgcatc attcgaacac
     1561 acacgcacac ctattcaaga aaatttattg gagtgtgcta tggtgtctgg tgtaaaaaag
     1621 gggaacgtta gagtatgttt taccgtaaat aaggaacacc tcaatcaaat agccgaatat
     1681 attgaagaat ataaaaaacc tattgaaaaa aaattatcag ttagtttaga tgtacattta
     1741 tctgttcagc atccttctac tcaaacagtt attgttaaag atgataattc tttttttcgc
     1801 gataaagata acaaaataac ctttacctat gcaggtcatg gagctttatt ggagaatttg
     1861 aatgcaattg atgctgaaat cgtatttatc aaaaatattg ataatgcagt tcccgacact
     1921 cttaaaaaaa caacatcaag tta
//
LOCUS       DAOSMY010000298         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_59247_length_1935_cov_3.561170, whole genome shotgun sequence.
ACCESSION   DAOSMY010000298 DAOSMY010000000
VERSION     DAOSMY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59247_length_1935_cov_3.561170"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..74
                     /locus_tag="PLD76_06375"
     tRNA            2..74
                     /locus_tag="PLD76_06375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Gly,seq:gcc)
     gene            124..207
                     /locus_tag="PLD76_06380"
     tRNA            124..207
                     /locus_tag="PLD76_06380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:158..160,aa:Leu,seq:gag)
     gene            complement(316..1308)
                     /locus_tag="PLD76_06385"
     CDS             complement(316..1308)
                     /locus_tag="PLD76_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27661.1"
                     /translation="MFFNTSCKDKSRFFINTQQTSTPISIERFDKDIYSLQSGNYTNK
                     IDSLYHTYGSFVDIYTQHILEIGLFRNTGESIVTTNATDEIRKMLQDSMVKVLYQDCF
                     KEYRTTKDVEQTLNTAFGYFQHYFPNKQLPRVLFHFSGFHQSIVFTDSVVSASVEYYL
                     GPDYPIYQQVAYQYEIPTMNRKQLPIDIVHAWIKRYYEIETPHERLLDQLIYEGKIRY
                     LLSIFFPEYTEYEIMGYSKEQYEWSVKNEKNIWAYIIENKRLFSNNWREIAGFINPGP
                     FTSGLSQESPGRIGVWIGFRIVQQYAENNKDVTLEKLMEDNNSQRILELSGYRP"
     gene            complement(1349..>1552)
                     /locus_tag="PLD76_06390"
     CDS             complement(1349..>1552)
                     /locus_tag="PLD76_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU27662.1"
                     /translation="GSFKNETNAKNLRNTLSKNGMNAFVAVNKDGMFRVFAVSSDDEK
                     TVRIELVKVRQTYPDAWILKLSK"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1563..1814)
                     /locus_tag="PLD76_06395"
     CDS             complement(<1563..1814)
                     /locus_tag="PLD76_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27663.1"
                     /translation="MKTKLIISLLIIGLATACGTKKNNLSAAYEVATETQTEVQTETN
                     DTTTAKIATEQAMSDAKITETKDRYQMENGQFVEKPFHII"
BASE COUNT          561 a          312 c          347 g          705 t
ORIGIN      
        1 tgcgaaaata gctcagttgg tagagcataa ccttgccaag gttagggtcg cgggttcgag
       61 tcccgttttt cgctctctaa aaatgcgaag cgtagaggtt tcgcattttc tatctgaaat
      121 attgcacaag tggtgaaatt ggtatacacg ctactttgag gtggtagtgg gcgaaagcct
      181 gtgtgagttc gagtctcatc ttgtgcacaa aaaagaggca gtcttagaat attttaagac
      241 tgcctctttt tattagggta tttgatgaag aatatattcc tagtagttta aaagtttatt
      301 cagacagctt cttttttatg gacggtagcc agataactct aaaatacgtt ggctattgtt
      361 gtcttccata agtttctcta aggttacatc cttattgttt tcagcatatt gttgcacaat
      421 acggaaacct atccaaaccc caatgcgtcc tggcgattct tgagagagtc cagaagtaaa
      481 aggaccagga ttaataaatc cagcaatttc acgccaatta tttgaaaaca atcgtttatt
      541 ttcaataata tatgcccaaa tatttttttc atttttcaca ctccattcat attgctcttt
      601 actatatccc attatttcgt attctgtata ctctggaaag aatatggata gaagatacct
      661 gattttgcct tcatagatta actgatcaag taaacgttca tgtggcgttt ctatttcata
      721 ataacgttta atccatgcat gtacaatgtc gattggtaat tgtttccgat tcattgtcgg
      781 aatttcatat tgataggcaa cttgttggta aataggatag tctggtccta gatagtattc
      841 aacgctggct gatacaacac tatctgtaaa aacaatagat tgatgaaagc ccgaaaaatg
      901 gaaaagtaca cgtggaagct gtttgttagg aaagtaatgc tgaaaataac caaatgccgt
      961 attcagtgtt tgttccacat ctttcgttgt acgatattcc ttaaaacaat cttggtaaag
     1021 tacttttacc atactgtctt ggagcatttt tcttatttca tcagttgcat ttgtggtaac
     1081 aatgctttct cctgtgtttc taaaaagacc tatttctaaa atatgttggg tgtaaatatc
     1141 cacaaatgaa ccatatgtat gatataagct atctatttta tttgtgtaat tacctgactg
     1201 gagcgaataa atatctttat caaaacgttc aatagaaatc ggcgttgaag tctgttgtgt
     1261 gttgataaaa aacctacttt tgtctttaca gcttgtgtta aagaacaaaa gtaggagtat
     1321 aatataaaag tttaaaaaaa tcctcaactt atttgcttag ctttaaaatc catgcatctg
     1381 gatatgtttg acgtactttt actaattcaa tacgaacggt tttttcatca tctgatgaaa
     1441 cagcaaatac gcgaaacatg ccatctttat ttacagctac aaaagcattc atgccgtttt
     1501 tagagagagt gttgcgtagg ttttttgcat ttgtttcgtt tttgaaacta ccnnnnnnnn
     1561 nnaataatgt ggaatggttt ttcaacgaat tgaccatttt ccatttggta acggtctttt
     1621 gtttcggtaa tttttgcatc agacatggct tgttctgttg ctatttttgc agttgttgta
     1681 tcatttgttt ctgtttgtac ttctgtttgt gtttcggttg ccacttcgta agctgctgat
     1741 aggttgtttt ttttagttcc gcaagctgta gctaatccaa tgataagtaa gctgataata
     1801 agttttgttt tcatgtgtgt gctgtttttt tatttgtata aaacgatgtt aaaaatcacc
     1861 acaaatatct gcattttttt tcatacaaaa aaacctataa gtttttttaa gaaaaaaagt
     1921 ctgttgtatt catca
//
LOCUS       DAOSMY010000299         1932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_59384_length_1932_cov_3.777837, whole genome shotgun sequence.
ACCESSION   DAOSMY010000299 DAOSMY010000000
VERSION     DAOSMY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59384_length_1932_cov_3.777837"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1932)
                     /gene="secA"
                     /locus_tag="PLD76_06400"
     CDS             complement(<1..>1932)
                     /gene="secA"
                     /locus_tag="PLD76_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798195.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU27664.1"
                     /translation="RVDCIDKHRPNSDDRRKAYLADITFGTNNEFGFDYLRDNMASTP
                     EDLVQRKHNYSIVDEVDSVLIDDARTPLIISGPIAKGEEQLYEQYCSKVERIVAEQKN
                     LVTKTFVEAKRLMQSADAKEQEKGSLLLFRAFKGLPKNKALIKYLSEPGVKASMLKTE
                     AIYMEQNNRRMHEVTDDLYFVINEQHNTIELTDKGIDLITGDSDDALFFVLPDIGSEM
                     AEIEKAKLSDEEKQAKKDVLMQEFSIKSERVHTINQLLKAYTLFEKDDQYVVMNNKVM
                     IVDEQTGRIMDGRRYSDGLHQAIEAKERVKVEAATQTYATITLQNYFRMYNKLAGMTG
                     TAETEAGEFWDIYKLDVVVIPTNRPIARVDMNDRVYKTARAKYNAVIEEVEKLVAQGR
                     PVLVGTTSVEISELVSKMLKLRKIPHNVLNAKLHQKEADIVAQAGKSGTVTIATNMAG
                     RGTDIKLTPESKAAGGLAIIGTERHESRRIDRQLRGRSGRQGDPGSSVFFVSFEDSLM
                     RLFGSDRIVGIMDRLGLQENEMMEHRQISNSIERAQKKVEENNFGIRKRLLEYDDVMN
                     SQREVIYARRRHALIGERIGIDIVNMIYDGVENLVNQHDDIQDEEEFRLDCLKLFGVD
                     IQLEEGEIKRTKTIDLVEK"
BASE COUNT          543 a          447 c          303 g          639 t
ORIGIN      
        1 ctttttctac taaatcaatt gtttttgtgc gtttaatttc gccttcctcc aattggatat
       61 ctaccccaaa cagtttcaaa caatccaaac gaaactcttc ttcatcttgt atatcatcat
      121 gttgatttac caagttttct acaccatcgt aaatcatatt cacaatatca atcccaatac
      181 gttcgccaat aagtgcatgt ctgcgacgag cataaatcac ttcacgttgc gaattcatga
      241 catcatcata ttctaacaaa cgcttacgaa taccaaagtt attttcttcg accttctttt
      301 gagcacgttc aattgaatta gatatctgac gatgttccat catttcattt tcttgcaagc
      361 ctaatctatc cataatcccc actatacggt cggatccaaa taaacgcatc aacgaatctt
      421 caaaagacac aaaaaatact gaagaacctg gatctccttg acgaccagaa cgaccacgta
      481 actgacggtc gatacgacgg ctttcatgac gttcggtacc aataattgcc aaacctcccg
      541 cagcttttga ttcaggagtt aatttaatgt cagtaccccg acccgccata ttggtggcaa
      601 tggtaactgt tccacttttc ccagcttgag caacaatatc cgcctctttt tgatgcaatt
      661 tagcatttaa tacattgtgt ggtattttgc gaagtttcag catcttactt accagctcgg
      721 atatttctac agaagtagta cccactaaca caggacgacc ttgtgctacc aatttttcta
      781 cttcctctat aactgcattg tatttggcac gagcggtttt atacactcga tcgttcatat
      841 ctacacgagc gataggacga ttcgttggaa tcacgacaac atctaactta taaatatccc
      901 aaaactcacc agcctccgtt tctgctgtac cagtcatacc tgctaacttg ttgtacatgc
      961 gaaaataatt ttgcaaagta atggttgcat aggtctgagt agcagcttcc accttcacac
     1021 gttctttggc ttctatcgct tggtgtaaac catcagaata acgtctaccg tccataatac
     1081 gacctgtttg ttcgtcaaca atcattacct tgttgttcat aacaacatat tggtcgtctt
     1141 tttcaaataa agtatatgct ttgagcaatt gattaattgt atgtacacgt tccgatttta
     1201 tcgagaactc ttgcatcaac acgtctttct tagcttgctt ttcttcatca gacagttttg
     1261 ccttttcgat ttcagccatt tcagagccaa tatctggaag cacaaaaaat aatgcatcgt
     1321 cgctatcgcc agtaattaaa tcaatacctt tatcagtcaa ttcaattgta ttgtgctgct
     1381 cgttgataac aaaatacaaa tcgtctgtaa cctcgtgcat acgacggtta ttttgctcca
     1441 tgtaaatagc ctctgttttc agcatagatg ctttcactcc tggctcactt aaatatttaa
     1501 tcagtgcctt attttttggc aaccctttaa aagctctgaa tagtaacaaa gagccttttt
     1561 cttgctcttt cgcatctgca gattgcatta atcgttttgc ttcaacaaat gttttagtaa
     1621 ctaggttttt ttgctcagct acaatacgtt ctacttttga gcagtattgc tcgtataatt
     1681 gctcttcccc ttttgcaata ggacctgaaa tgataagagg tgtacgagca tcatcaatca
     1741 gtacagagtc cacctcatct acaatggagt agttatgttt tcgttgtact aaatcctcgg
     1801 gagtagatgc catattatcc cgtaagtaat cgaagccaaa ctcgttgttg gtaccaaagg
     1861 taatatcagc caaatacgct tttcgtctgt catctgaatt gggacgatgt ttatcgatac
     1921 aatctacacg ta
//
LOCUS       DAOSMY010000300         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_59514_length_1929_cov_2.927962, whole genome shotgun sequence.
ACCESSION   DAOSMY010000300 DAOSMY010000000
VERSION     DAOSMY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59514_length_1929_cov_2.927962"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1094)
                     /locus_tag="PLD76_06405"
     CDS             complement(99..1094)
                     /locus_tag="PLD76_06405"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOU27665.1"
                     /translation="MKAKRICIMGGGSWATALAKITMSKGDKINWYMRRPEQIEIFQR
                     LGNNPSYLSSIRFDTKKIKFYADINLAVKNSDILVFATPSPFLKQHLQKLTRTLSNKI
                     IVSAIKGIVPDENMIINDYFQKFYGVKQENIAIIAGPCHAEEVALERLSYLTIACANN
                     ELGTYLAERLSCSFLKTSITSDINGIEYSSVLKNVYAIAVGICHGLKYGDNFQAVLIS
                     NSIREINRFVDAVERNNERNTQDSAYLGDLIVTAHSRFSRNRTFGTMIGKGYSVKTSQ
                     LEMEMIAEGYYGTKCIKEINDTLNVDMPILDAVYNILYERIAPTIEIKLLTEKIR"
     gene            complement(1108..>1929)
                     /locus_tag="PLD76_06410"
     CDS             complement(1108..>1929)
                     /locus_tag="PLD76_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOU27666.1"
                     /translation="NLNNNIENTRRKIINFKAPFKRISMTDAIKEHTGFDITGKNEKE
                     LREICNALHIETDETMGIGKLIDEIFGEKCEGNYIQPTFITDYPIEMSPLTKKHRSKA
                     GLTERFELMVNGKELANAYSELNDPIDQRERFEEQLRLSEKGDDEAMFIDQDFLRAME
                     YGMPPMSGIGIGMDRLVMAMTNQTSIQEVLLFPQMRPEKIAKKDTPARFIELGMSEEW
                     AQAIVKHSSLSIKDIASNIDAKLHQQLCGINKKHKLSLPNPTLADVQVWIKKASE"
BASE COUNT          560 a          325 c          294 g          750 t
ORIGIN      
        1 actattgcat gcacttactt ctttttcgta agcaaatact ttatcttttg atataaaacc
       61 taaggtattg tcaattacca atttaatgtt ttccattttt atcgtatttt ttctgttaat
      121 aatttaattt cgattgttgg ggctatgcgt tcgtacaata tattatatac tgcatcaaga
      181 ataggcatat ctacatttag agtatcatta atttctttaa tacatttagt tccataataa
      241 ccttctgcaa tcatttccat ttctaattgt gaagttttta cagaatatcc ttttcctatc
      301 atggtgccaa aagtacgatt acgactaaaa cgagaatgtg ctgttactat taaatcgcct
      361 aaataggctg aatcttgcgt attacgctca ttattccgtt ctactgcatc tacaaaacga
      421 tttatttcac gaatactatt tgaaattagt actgcctgaa agttatcacc atattttagt
      481 ccatgacata ttcctactgc aatagcataa acatttttga gaacagatga atattcaatg
      541 ccattaatat cgcttgtaat agatgttttt aagaatgaac aacttaatct ttcggctaaa
      601 tatgttccta attcgttatt agcacatgca atggttaagt atgaaagacg ctctagagca
      661 acttcttctg catgacaagg tccagcaatg atagcaatat tttcttgttt aacaccatag
      721 aatttttgaa aataatcatt aataatcata ttttcatcag gaactattcc tttgattgca
      781 gatacgatta ttttattact taatgtacgt gttagttttt gtaaatgttg tttaagaaaa
      841 ggagagggag ttgcaaaaac taaaatatca gagtttttaa ctgctaagtt aatgtctgca
      901 taaaatttta ttttttttgt atcaaatcta atcgaactga gataggaagg attatttcct
      961 aagcgttgga atatttcaat ttgctcaggt cttctcatgt accaattaat tttatctcct
     1021 tttgacatgg taattttggc taaagcggtt gcccagcttc cacctcccat aatgcatatt
     1081 cgttttgctt tcatttgttc catactgtta ttcgcttgct ttttttatcc agacttgtac
     1141 gtctgctaaa gtaggattag gtaaggatag tttgtgtttt ttattgatac cacacagttg
     1201 ctgatgcaat tttgcatcaa tattgcttgc aatatctttg atagaaagag acgaatgttt
     1261 tactatagct tgtgcccatt cttcagacat gccaagttct ataaatcgtg caggggtatc
     1321 ttttttagca atcttttcag gacgcatttg tggaaataat aatacttctt gtatactggt
     1381 ttgattggtc atagccataa ctaatcgatc cattcctata cctataccag acatgggtgg
     1441 cattccatat tccattgctc ttaaaaaatc ttgatcgatg aacatagctt catcatcacc
     1501 tttttcactt aaccgaagtt gttcttcaaa tcgttctcgt tgatcaatag ggtcatttag
     1561 ttcagaataa gcatttgcaa gttcttttcc atttaccatt aattcaaaac gttcggttaa
     1621 tcctgcttta gaacggtgtt tttttgtgag aggagacatt tcaattgggt agtctgtaat
     1681 aaatgtaggt tgaatataat tgccttcaca tttttcccca aaaatttcgt caataagctt
     1741 tccaatgccc attgtttcat cggtttctat gtggagtgca ttacatattt cacgtaattc
     1801 tttttcattt ttacctgtta tatcaaaacc tgtatgctct ttaatagcat cggtcattga
     1861 aattcgtttg aatggggctt taaaattaat tattttgcga cgtgtatttt ctatattgtt
     1921 atttagatt
//
LOCUS       DAOSMY010000301         1929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_59535_length_1929_cov_2.143543, whole genome shotgun sequence.
ACCESSION   DAOSMY010000301 DAOSMY010000000
VERSION     DAOSMY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59535_length_1929_cov_2.143543"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="infC"
                     /locus_tag="PLD76_06415"
     CDS             complement(<1..334)
                     /gene="infC"
                     /locus_tag="PLD76_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOU27667.1"
                     /translation="MAKVFTPRPNRNFQKKEEAHKTNEAIHHPEVRLVGENVEPGIYT
                     LAQAKHIADELNLDLVEISPNAVPPVCRVIDYTKFLYQQKKKEKELKAKTAKVTVKEI
                     RFGPQTDEH"
     gene            complement(446..853)
                     /locus_tag="PLD76_06420"
     CDS             complement(446..853)
                     /locus_tag="PLD76_06420"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HOU27668.1"
                     /translation="MKKLLITLFLAVSTVVFSFAQNELPEKARIFLETHFSNIQIQKV
                     SRVDIEQVQIFKVLLADKTQIEFDQTGAWTKINYKKSPKTISYLPETLQQEIESNKQI
                     KKVREIETDGYDIAIQLKNKKQVKGNRFGKLEK"
     gene            complement(874..>1929)
                     /gene="thrS"
                     /locus_tag="PLD76_06425"
     CDS             complement(874..>1929)
                     /gene="thrS"
                     /locus_tag="PLD76_06425"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOU27669.1"
                     /translation="IGQKELWITSGHYAKYGKDSFQPINTPQEGEEYLLKPMNCPHHC
                     EIYKVSPRSYKDLPIRYAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCRPDQLKDE
                     FMKVMDIIFIIFKALNFENFEAQISLRDPNNKEKYIGSDENWEKSEQAIIESCEAKGL
                     KARVELGEAAFYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFELEYTGADNQKHRPV
                     MIHRAPFGSMERFVAVLIEHTGGKFPLWLTPEQVVILPISEKFNDYAKEVVNTLNMHD
                     IRASVDERNEKIGKKIRDNELKRIPYMVIVGEKEAENNELSVRKQGEGDKGTYSINDF
                     AQLIVDEVNNMTNKFLA"
BASE COUNT          494 a          356 c          341 g          738 t
ORIGIN      
        1 catgttcgtc agtctgtgga ccgaaacgaa tctctttgac tgtaacttta gccgttttag
       61 cttttagttc tttctccttc tttttttgtt ggtacaagaa ttttgtatag tcaattacac
      121 gacaaactgg cggtactgca ttaggcgaaa tctcaactaa atcaagatta agttcatccg
      181 ctatatgctt tgcttgtgct aatgtgtaga taccgggctc tacgttttct cctactagac
      241 gaacttcagg atgatgaata gcctcattgg ttttgtgggc ttcttctttt ttctgaaagt
      301 ttctattggg tctcggtgta aaaacttttg ctatagtaaa cgtcctccta aattaaataa
      361 ttactaaatg cttatttttc agcccgcaaa tataggcttt ttttatgata tatcacaatt
      421 tatcgttaaa aaaatagtgg tttttttatt tttcaagttt accgaatcta tttcctttca
      481 cttgtttttt atttttaagt tggatagcta tatcatatcc atctgtttct atttctctta
      541 ccttttttat ttgcttattg ctctctattt cttgttgtag agtttctggt aagtatgaaa
      601 tagttttcgg tgattttttg taattaatct tggtccaagc tcctgtttgg tcgaattcta
      661 tttgtgtttt atctgctaat aaaaccttga atatttgtac ttgttctatg tcaacgcgac
      721 ttactttttg aatttggata ttgctaaaat gagtctctag aaaaatacgt gctttttcag
      781 gtaattcatt ttgagcaaaa ctaaaaacta cagtacttac tgcaaggaat agggttatta
      841 gtaatttttt catgagttca gtctattaat tgattatgct aaaaatttgt tagtcatgtt
      901 atttacttca tctactatta gctgtgcaaa atcgttgatg gaataggttc ctttatcgcc
      961 ttcgccttgt ttgcgtacag atagttcgtt gttttcagct tctttctcgc caacgattac
     1021 catatacggt atgcgtttta attcgttgtc acgaattttt ttaccaattt tttcgttgcg
     1081 ttcgtccacc gaggcacgta tatcatgcat attaagcgta ttcaccactt ctttggcata
     1141 atcgttaaac ttttcgctaa ttggtaaaat aacaacttgt tcgggcgtta gccataaggg
     1201 aaacttgcct cctgtatgtt caataagaac agccacaaaa cgctccatag agccaaatgg
     1261 ggcacggtga atcattacag gacggtgttt ttgattatca gcccctgtat attccaattc
     1321 aaaacgctcg ggtaggttat agtccacctg aatggtgcct aactgccaac gacgaccaat
     1381 ggcatctttc accataaaat cgagttttgg accatagaag gctgcttctc ctagttcaac
     1441 acgtgctttt agcccttttg cttcgcacga ttctatgata gcttgttcag atttttccca
     1501 gttttcgtca ctaccgatgt atttttcttt gttatttggg tctcgcaacg agatttgtgc
     1561 ttcaaagttt tcgaaattca atgctttgaa aataatgaag ataatgtcca ttactttcat
     1621 aaattcgtct ttaagctggt cgggacgaca gaaaatatgt gcatcgtctt gtgtaaagct
     1681 gcgaacgcgt gttagtccat gtaactcacc actctgttcg tatcgataaa cagtaccaaa
     1741 ttcagcatat cgtatgggta aatctttgta cgaacgtgga gataccttat atatttcgca
     1801 atggtgagga cagttcattg gtttaagtaa atattcttcg ccttcttgtg gtgtgttgat
     1861 tggttgaaat gaatctttac catattttgc ataatgtcca gatgtaatcc acaattcctt
     1921 ttgtccgat
//
LOCUS       DAOSMY010000302         1928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_59570_length_1928_cov_2.527496, whole genome shotgun sequence.
ACCESSION   DAOSMY010000302 DAOSMY010000000
VERSION     DAOSMY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59570_length_1928_cov_2.527496"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PLD76_06430"
     CDS             <1..966
                     /locus_tag="PLD76_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOU27670.1"
                     /translation="KYCFLKPAGIECNEEWLENDFEHLASFLLQDKSNTFMYRDFQSR
                     NVMIRNGNPYFIDFQGGRKGPIQYDVASFVYQAKANFSAEVRETLIASYLEALSKYMP
                     VDEVAFRKQLAFFVLFRNLQVLGAYGFRGLVEKKKHFVESIPFAIQNLNEILRNNYFD
                     THFPYLVSVLQQLIVCYTHQDKPTSPSNLVVKVSSFSYKKGIPEDTTGNGGGYVFDCR
                     AIHNPGKYEQYKQLTGLDKPVIDFLEQDGEILAFLEHIYALADSSVSRYVERGFSSIM
                     FSFGCTGGQHRSVYAAQHVAEYIATRYSVKVELVHREQGIKQQFN"
     gene            979..1716
                     /locus_tag="PLD76_06435"
     CDS             979..1716
                     /locus_tag="PLD76_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU27671.1"
                     /translation="MKAMIFAAGLGTRLKPLTDSMPKALVPINGKPLLEHVLLKLKQE
                     GFTDIIINVHHFADQIVQFLAQNQHFGLNIAISDETQQLLETGGGIKKASWFFGNEPF
                     LIHNVDILSNLPLAELVRFHTNKPDAAATLVVSKRTTTRYLLFDNDMQMKGWTNIQTN
                     EIKSSYPQTIIQACSEFAFSGIHVFSPALFPYFEAYPAKFSVIDFYIDVCGKAAVYGY
                     VPTNLQLLDVGKIDSIQQAELFVQTMY"
BASE COUNT          593 a          333 c          390 g          612 t
ORIGIN      
        1 aaatattgtt ttttaaaacc tgctggtatc gaatgtaacg aagaatggtt agaaaatgat
       61 tttgaacatc tggcatcatt cttgcttcaa gataaaagta acacgtttat gtatcgcgat
      121 tttcagagtc gcaatgtgat gatacgcaac ggaaatcctt attttattga ttttcaggga
      181 ggacgaaaag gtccaattca atacgatgta gcttcgtttg tgtatcaagc caaagccaat
      241 ttttcggcag aagttcgtga aaccttgatt gcaagctatt tggaggcatt gtcgaagtat
      301 atgcctgtag atgaggtggc ttttcgcaaa caacttgcat tttttgtgtt atttaggaat
      361 ttgcaagtac ttggagccta cggttttagg ggattagtgg agaagaaaaa gcattttgta
      421 gaaagcatcc cttttgctat tcagaattta aatgaaatcc tcagaaataa ttattttgat
      481 actcattttc cgtatcttgt tagcgtatta cagcaattaa tcgtttgcta tacacaccaa
      541 gataagccaa ccagcccatc gaacttagtg gtaaaggtaa gtagtttttc gtataaaaaa
      601 ggtatacccg aagatacaac gggcaacgga ggtggttacg tctttgattg tagggctatt
      661 cataatcctg gtaagtacga gcaatataag caactaacag gattagacaa acccgtgatt
      721 gattttttgg aacaagatgg cgaaatttta gcctttcttg agcatattta tgcattagct
      781 gatagttcgg taagcaggta tgtagaaaga ggtttttctt caattatgtt ttcttttggt
      841 tgcaccggag gacaacatcg ctctgtatat gcagcacaac atgtagccga atatatagct
      901 acacgctatt ctgtaaaagt ggaattagta catcgggaac aaggcattaa acaacagttt
      961 aattagaata ccatacgtat gaaagcgatg atttttgcag caggattggg tacacggttg
     1021 aaaccgctta ctgatagtat gcccaaagct cttgtaccca ttaatggaaa accgctactt
     1081 gagcatgttt tgcttaaact caaacaagag ggttttacgg atatcatcat caacgtgcat
     1141 cattttgctg atcagattgt tcagtttttg gctcaaaacc aacattttgg actcaacatt
     1201 gccatctcag acgaaacaca acaattactc gaaacaggag gaggcatcaa aaaagcatct
     1261 tggttttttg gcaacgaacc ttttttgata cacaacgtag atatcttgtc gaatcttccc
     1321 ttagctgaac ttgtacgatt tcataccaat aaacccgatg cggcagccac tttggtggta
     1381 agtaaacgta ctacaacgcg ttacttgttg ttcgataatg atatgcagat gaaagggtgg
     1441 acgaatatac aaaccaacga aataaaatct tcttatccac aaactattat acaagcctgt
     1501 agtgagtttg catttagtgg cattcatgtg ttttcgcccg cattatttcc ctattttgag
     1561 gcttaccccg ctaaattttc tgtaattgat ttttacattg atgtatgtgg caaggctgct
     1621 gtgtatggct acgtgcctac caatttgcag ttgttagatg taggaaaaat tgacagtatt
     1681 cagcaagctg aattgtttgt acaaactatg tactgatttt ttgtcagatt atgtattaaa
     1741 aattttttac gatttctgcc tgctgggtta atacccgtta atgcccaaaa tcaaagtatt
     1801 aaaaattgat aaaaccctta aaaacaagtt ttcggatggg cctgcaaatg gcactcacgg
     1861 gggggctaat aaaatctaca ttgatagtgt tttggttttc tcaaacgaca gttcagttaa
     1921 tattgaag
//
LOCUS       DAOSMY010000303         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_60216_length_1913_cov_2.459096, whole genome shotgun sequence.
ACCESSION   DAOSMY010000303 DAOSMY010000000
VERSION     DAOSMY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60216_length_1913_cov_2.459096"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PLD76_06440"
     CDS             <1..697
                     /locus_tag="PLD76_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27672.1"
                     /translation="FKTDAKKYGYNFDDKGLIVRFADLDNNIAGLAYYKRNPILIEID
                     REYWASASNTKNAHDIKENLLFHELGHGFLQRMHDNTVLANGDWKTIMCGDKLPNDRA
                     SNINYRGFRKAYYIEELFTRTNDTPAWSTLIPQFDNIDENVILQQDFSSGSDWTIGSN
                     SLYESSIENGAYTFTTKTSQAFYVLNKGTLNTSNDFYVEVRLKASAGLDDSFGLVCGS
                     FNDGNTPTSLHYF"
     gene            716..1045
                     /locus_tag="PLD76_06445"
     CDS             716..1045
                     /locus_tag="PLD76_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27673.1"
                     /translation="MYIGESECLGPFIDLYTEILHPNEFNTFAIRKYNNMLYYYINDT
                     FIYHNDLDEIITMYGSQIGFKIPGNSTLYVDYAEVRENSTGLKKRNTTELSVEKATEK
                     KVIHWNK"
     gene            complement(1050..1649)
                     /locus_tag="PLD76_06450"
     CDS             complement(1050..1649)
                     /locus_tag="PLD76_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU27674.1"
                     /translation="MSRTRVIHTTITSTRELLLEVARQLFAKVGFTNTTMNDIAKAAK
                     RGRRTLYVYFKSKEDVYYAVVEQEMYKLLDALELVANSAEPPQNKLIDYIYTRLDKTR
                     EAVKNNGTLRADFFSDYKTVEMVRKKMEIKEYELLKKILKEGVDAGIFYVRSIDFAAF
                     LVLNAIRGLELPYMKQSVADRINDSREDIVRVLFHGLLK"
BASE COUNT          658 a          352 c          296 g          607 t
ORIGIN      
        1 gtttaaaaca gacgccaaaa aatatggata taattttgat gacaaaggac taattgtacg
       61 ttttgctgat ttagacaaca atattgcagg cttagcttat tacaaaagaa atcctatttt
      121 aattgaaatt gatagagaat actgggcatc ggctagtaat acaaagaatg cacatgatat
      181 taaagaaaac ctactgtttc atgaattagg gcatggattt ttgcaacgta tgcacgataa
      241 tactgtatta gcaaatggag attggaaaac aattatgtgt ggcgataaac tcccaaacga
      301 cagagcctct aacatcaatt atagaggttt tagaaaagca tactatatcg aagaactatt
      361 tactcgcacc aacgatacgc cagcatggag tacactcatc cctcaatttg ataatatcga
      421 cgaaaacgtc attctacaac aagatttttc tagtggttca gattggacaa taggctccaa
      481 tagtttatac gaaagttcca ttgaaaatgg agcatataca tttaccacca aaacttctca
      541 ggctttttat gtattaaaca aaggtactct gaatacaagc aatgattttt acgtagaggt
      601 tcgactcaaa gcatcagcag gtcttgatga ctcatttgga cttgtttgtg gctcatttaa
      661 cgatgggaat acgccaactt ctcttcatta tttttaacaa aaaggaaata atcatatgta
      721 tataggagag tccgaatgtt taggtccatt tattgattta tatacagaaa ttttacatcc
      781 taacgaattc aacacatttg ctatacgtaa atacaataat atgttgtact attatatcaa
      841 cgacaccttt atctatcaca acgatttaga tgagattatc accatgtatg gctcacaaat
      901 tgggtttaaa attcctggca atagcaccct atatgttgat tatgcagagg tcagagaaaa
      961 tagcactggg ttgaaaaaac gaaataccac cgaacttagt gtggaaaaag caacggagaa
     1021 aaaagtcatt cattggaata agtaacaggc tacttcaata acccatgaaa caacacacga
     1081 acgatatctt ccctgctatc gtttattcta tcagctacag actgtttcat atagggtagc
     1141 tctaatcctc taattgcatt caatactaaa aaagcagcaa aatcaatact tctaacataa
     1201 aagattccag catcaacacc ttctttaaga attttcttta gtaattcata ttctttgatt
     1261 tccatttttt tacgtaccat ttccaccgtt ttataatcac taaaaaaatc agctctcagt
     1321 gtaccgttat ttttaacagc ctctctggtt ttatctaatc gtgtatagat ataatcaatc
     1381 aatttatttt gaggaggttc ggcagaattt gctactaact ccaaagcatc cagcaattta
     1441 tacatttcct gttctactac tgcataataa acatcttcct tgcttttaaa ataaacgtat
     1501 aaggttcgcc tgccccgttt agccgcttta gcaatatcat tcatagtagt attcgtaaaa
     1561 ccgactttag caaacaattg cctggcaacc tctaacaata actccctagt tgacgtaatg
     1621 gttgtatgta taactctagt tctactcatt gcacaataat gttactttgt gcaaaaataa
     1681 gggagttatt tcaatttatc aaataattaa gcattttttt tattattcct ccaaaaacgt
     1741 aaaaaatata tcgtactttt gcatatatag catatgatac atctagtaac acactacata
     1801 catacacaca aattactctc tcctgcagct acttttagag ttggggtaag tgggggaggc
     1861 gattctatgg cattactcta tattctcaaa gaaatcggat acaattgtgt ggt
//
LOCUS       DAOSMY010000304         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_60463_length_1907_cov_4.129050, whole genome shotgun sequence.
ACCESSION   DAOSMY010000304 DAOSMY010000000
VERSION     DAOSMY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60463_length_1907_cov_4.129050"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLD76_06455"
     CDS             <1..409
                     /locus_tag="PLD76_06455"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOU27675.1"
                     /translation="QALAEAKKAFAKGEVPIGAVVVCNQQIIARAHNFTETLHDVTAH
                     AEMQAITAAASYLGAKYLNDCTLYVTVEPCVMCAGALYWSKVAKVVYGASEDKYGYTK
                     KVPLALHPKTEVVAGVMADECAQIMKDFFITKR"
     gene            414..1706
                     /gene="tyrS"
                     /locus_tag="PLD76_06460"
     CDS             414..1706
                     /gene="tyrS"
                     /locus_tag="PLD76_06460"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU27676.1"
                     /translation="MNFVDELEWRGMIHTVMPGTKEQLEKELTSAYVGIDPTADSLHI
                     GHLVGVMMLKHFQRCGHKPIALIGGATGMIGDPSGKSAERNLLDEKTLRHNQDAIKQQ
                     LARFLDFDSDAPNAAEIVNNYDWMKSFTFLDFIRDVGKHITVNYMMAKDSVKKRLSSE
                     SSVGMSFTEFTYQLVQGYDFLYLYDAKNCKLQMGGSDQWGNITTGTELIRRKVGGEAF
                     ALTCPLITKADGGKFGKTESGNVWLDAKYTSPYHFYQFWLNVSDEDAQKYIKIFTFIS
                     KEEIESLVATHLEAPHLRLLQKRLAQEVTCMVHSQEAYEMAVEASAVLFGNATSETLK
                     KIDTATLLAIFEGVPQFEIDTNDLSGEVKLMDLLTTQAPVFASKGEMRKMIQNGGLFI
                     NKEKITDTEAIATSNLLLNNTFILVQKGKKNYYLLRVN"
BASE COUNT          623 a          342 c          400 g          542 t
ORIGIN      
        1 acaagcccta gcagaggcta aaaaagcgtt tgctaaagga gaagttccta ttggtgcggt
       61 ggtggtatgt aaccaacaga ttattgcccg tgctcataat tttaccgaaa cattgcatga
      121 tgttaccgcc catgccgaaa tgcaagcaat tactgctgct gcaagttatt taggagctaa
      181 atacctgaat gattgcactt tgtatgtaac agtagaaccc tgtgtaatgt gtgctggtgc
      241 attgtactgg tcaaaagtag ccaaagtagt atatggagca agcgaagata aatatggata
      301 taccaaaaaa gttcctttag ccctgcatcc taaaacggaa gttgtggctg gcgtgatggc
      361 agatgaatgt gctcaaataa tgaaagattt ttttataact aaacgataat aacatgaatt
      421 ttgttgatga attagaatgg agaggtatga tacataccgt aatgccaggt acaaaagaac
      481 aattagaaaa agaattaacc tctgcttatg taggtattga cccaacggca gattccttgc
      541 atataggtca tttggtgggt gtaatgatgc tcaagcattt tcaacgttgc ggacacaaac
      601 ccattgcctt aataggaggt gcaaccggta tgattggcga cccatcgggc aaaagtgctg
      661 agcgtaactt gcttgatgaa aagaccttac gtcacaatca agatgcaatt aagcaacaat
      721 tagcccgttt tctcgatttt gatagcgatg cccccaatgc agccgaaatt gtaaacaatt
      781 acgattggat gaaatccttt acgtttttag attttatcag agacgtgggc aaacacatca
      841 cagtgaatta tatgatggca aaagattctg taaaaaaacg cctgtcttcc gaatcttctg
      901 taggcatgtc gtttacggaa tttacttatc aattggtaca aggatacgac tttttatatt
      961 tatacgatgc taaaaactgc aaactacaaa tgggtggctc agaccaatgg ggcaatatta
     1021 caactggtac ggagttaatt cgcagaaaag taggtggaga ggcttttgca cttacttgtc
     1081 cgcttattac caaagccgat ggtggcaaat ttggtaaaac agaaagtggc aatgtgtggt
     1141 tagatgcaaa atatacctca ccatatcatt tttatcagtt ttggctcaat gtaagcgatg
     1201 aagatgctca aaaatacatc aaaatattta cgtttatttc caaagaagaa attgagagtt
     1261 tagtagcaac gcacctcgaa gcacctcatt tgcgactatt gcaaaaacgc ttggcacaag
     1321 aagttacctg catggtacat tcgcaagaag cgtacgaaat ggcagtagaa gcctcggctg
     1381 tgttgtttgg taacgccact tccgaaacgc ttaaaaagat tgataccgcc accttattgg
     1441 caatattcga aggcgtgcct caatttgaaa ttgatacaaa cgacttgtcg ggagaagtaa
     1501 aactgatgga tttacttacc acacaagctc ctgtatttgc ctcgaaaggc gaaatgcgta
     1561 aaatgataca aaacggaggc ttgtttatca ataaagaaaa aattaccgat accgaagcca
     1621 tcgctactag caatttatta ctcaacaata cctttatatt ggtacaaaaa ggcaaaaaga
     1681 actactattt gctccgtgtg aattgatttt tttagaaaaa tatttggtga attaaatctt
     1741 acatactacc tttgcaccac atttgcgata aggtaacttt tgcaatagat ttgattgtct
     1801 catggtgtaa tggtagcact acagtttttg gttctgtctg tctaggttcg aatcctggtg
     1861 agacaacaaa gaaaaaagct atttacttgt aattcaggta gatagct
//
LOCUS       DAOSMY010000305         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_60471_length_1907_cov_3.276998, whole genome shotgun sequence.
ACCESSION   DAOSMY010000305 DAOSMY010000000
VERSION     DAOSMY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60471_length_1907_cov_3.276998"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /gene="sprA"
                     /locus_tag="PLD76_06465"
     CDS             <1..1803
                     /gene="sprA"
                     /locus_tag="PLD76_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU27677.1"
                     /translation="QNLGSTIYNQGAWNVDGRFNFEMLYNKSKYLREINRKYGSSRPQ
                     PTPQSTTAPKKFEQIITLKKGAKVVVQHNLNDLKPIVKNLADGKVYSIRYTTIDANSL
                     SFVPQTDGEVSLAFEQRSSQQPDFSTQTLAYSSRFLMMLRNMSFTYNETEGTSIYGFK
                     PGVTLFGLDKYNGRMAPGWDFVFGLQDENFVNRAMANDWLVLSNALNPAVYKHTQEFQ
                     IRALVEPIAGFKINIVADRVAVKDKQVQFSYADMPSTVGGNFSMSYLAIATAFSSIAD
                     DGSFTSDVYNQFVQNRALIAARLEEKRQGTLYPDKGFISDASLGNSVYDPTISSYSLN
                     SPDVLIPAFLAAYSGADVRKVSLSPFASFWNLMPNWQVSYDGLSRIAWINDNFRSVNL
                     RHAYSAKYTVGAFTSYLTYVGDSEDNGYIKDVLTGNPIASSQYSISSVSINELFNPLI
                     GIDLNMKNSLSTKVEIRKGRNLVLNLASNQLVETLNDEIVVGMGYKISDFDVILRLQS
                     NQQKKVKNDLNLRADFSIRDNKTIVRKIEEAYAQATSGNMILSVKFLADYVFSERINI
                     SFYYDLQANNPVVMTSYPISNSNVGFSVKMLLTR"
BASE COUNT          611 a          304 c          374 g          618 t
ORIGIN      
        1 caaaacttag gaagtacaat ttacaatcaa ggtgcttgga atgtggatgg acggtttaat
       61 tttgaaatgc tgtataataa atctaaatat ctgcgcgaaa tcaatagaaa atatggtagt
      121 agtcgtcctc aaccaactcc acaatcaact actgccccta agaagtttga acagattata
      181 accctaaaga aaggagcaaa agttgttgtt cagcataatc ttaacgacct aaaacctatt
      241 gttaagaatc ttgcagatgg aaaagtatat tccattcgtt acactacaat tgatgctaat
      301 tctctttcgt ttgtgccaca aactgatgga gaggtttcgt tggcttttga acaacgttct
      361 tctcagcaac ctgatttttc gacccaaacg ttggcttatt cttctcgctt tttaatgatg
      421 ttgcgaaata tgtcatttac gtataatgaa acagaaggaa cttctattta cgggtttaaa
      481 ccaggtgtaa cactatttgg tttagataag tataatggta gaatggctcc tggatgggat
      541 tttgtgtttg gtttgcaaga cgaaaatttt gtaaacagag ctatggcaaa tgactggtta
      601 gtattgagta atgcattaaa tccagcggtg tacaaacaca cacaagaatt tcagataagg
      661 gcgttagttg aacctattgc aggtttcaaa ataaatattg tagcagatcg cgttgcagta
      721 aaagataaac aagtgcaatt ttcgtatgca gatatgccat caacggtggg tggaaacttt
      781 tctatgagct atcttgctat tgccacagca tttagttcta ttgctgatga tggttctttt
      841 acttcagatg tgtataatca atttgtgcag aatagggctt tgatagctgc aaggttagaa
      901 gaaaaacgac agggaacgct ctatcccgac aaaggattta tatctgatgc aagtttggga
      961 aatagtgtct atgatccaac tatcagttct tattctctca attcacctga tgtgctcatc
     1021 cctgcttttt tagccgctta ttcgggtgct gatgtgcgaa aagtatcgtt aagtcctttt
     1081 gcttcctttt ggaacttaat gcctaattgg caagtttctt atgatggatt atctcgtatt
     1141 gcatggataa atgataattt ccgtagtgtg aatttgcgac atgcctatag tgcaaaatat
     1201 acggttggag catttacatc ctatttaacc tatgtgggag acagtgaaga caatggctat
     1261 attaaagatg tgttaactgg taatcctatt gcctcgtcac aatacagcat aagtagtgtg
     1321 tctatcaacg aactttttaa tccattaatt ggtattgacc tcaatatgaa aaacagttta
     1381 tcaaccaaag tagaaatacg taaaggaaga aatcttgttc tgaatttagc aagtaaccag
     1441 ctagtggaaa cattgaatga tgaaattgtg gtaggaatgg gatataaaat tagtgatttt
     1501 gatgtgatat tacgtttgca atcaaatcaa caaaaaaagg tgaaaaatga cctcaattta
     1561 cgtgcagatt tctctattcg agataataaa actattgtac gaaaaattga agaagcatat
     1621 gctcaggcta ctagcggtaa catgattttg agcgttaagt ttttggcaga ctatgtattt
     1681 agtgagcgta taaatatcag tttttactac gatttacagg ctaataatcc tgtggttatg
     1741 acatcatatc ctatctccaa ttccaatgta ggatttagtg tcaagatgct tctaactaga
     1801 taaaaaatta ctttggggct attttataaa ggtataatcc aattaaaaaa tagacaacat
     1861 tgggcgacca tatggctacc attggattaa atcctgcttt tactgtt
//
LOCUS       DAOSMY010000306         1900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_60791_length_1900_cov_2.911653, whole genome shotgun sequence.
ACCESSION   DAOSMY010000306 DAOSMY010000000
VERSION     DAOSMY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60791_length_1900_cov_2.911653"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..76)
                     /locus_tag="PLD76_06470"
     CDS             complement(<1..76)
                     /locus_tag="PLD76_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU27678.1"
                     /translation="MAHLITEDCIACGTCISECPVEAIS"
     gene            287..1558
                     /locus_tag="PLD76_06475"
     CDS             287..1558
                     /locus_tag="PLD76_06475"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOU27679.1"
                     /translation="MKVDVLLGLQWGDEGKGKVIDVLTPNYDVIARFQGGPNAGHTLE
                     FDGKKFVLHTIPSGIFQGDKINIIGNGVVIDPIVLKNEIEKLEKAGYDLSQKLVISKK
                     AHLILPTHRLIDAAQEAQKGDAKIGSTLKGIGPTYADKISRTGLRVGDLLHNFTEKYA
                     KKIADHTRLLDQMGFSYSLDGLEDEWMEATKTLLRFQLIDSEHVVNELIADNKRILAE
                     GAQGTMLDIEFGSYPFVTSSNTVSAGVCTGLGVPPTQIGEVYGIMKAYCTRVGSGPFP
                     TELFNEEGEEMRKKGNEFGSTTGRARRCGWIDLVALKYAIMINGVTQLIMMKSDVLDQ
                     FDVIKACVGYKIDGEEYDYFPYEINANLEPMYVELPGWKTDMSKMQKESQFPQAFNDY
                     LDFLEDELEVPIRIVSVGPDREQTILRYINE"
     gene            1643..>1900
                     /locus_tag="PLD76_06480"
     CDS             1643..>1900
                     /locus_tag="PLD76_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOU27680.1"
                     /translation="MIIYVIFGVVALSSWLIQYRLKSKFEKYSKLALGNGMSGKEVAE
                     KMLRDNGISDVTVISTSGHLTDHYNPTNKTVNLSEAVYNSAS"
BASE COUNT          671 a          297 c          383 g          549 t
ORIGIN      
        1 cagagatagc ctctacagga cattcgctaa tacatgtacc acaagcgata caatcttctg
       61 taattaagtg agccattttt tctttattta aaagttaacg tgttgcaaaa gtacattatt
      121 tttttataac acaaaaatta ctatccattt tttgaaaaaa aaagacaact ctaccgttga
      181 aaagttaaaa tataatggta tataataaaa aaatagtatc tttacgggtt caaaaaaaat
      241 aagaacataa tttttatacg caaaaacaat attgaagtaa aaaagaatga aagtagatgt
      301 tttattaggc ttgcaatggg gagatgaagg caaagggaaa gtgattgatg tattaacccc
      361 taattatgat gtaattgccc gatttcaagg aggaccaaat gcgggacata cattagaatt
      421 tgatggtaag aaattcgtat tacatactat cccctctggt atttttcaag gagataaaat
      481 aaatattatt ggaaatggag ttgttattga tcccattgtc ttgaaaaatg aaatagagaa
      541 actagaaaaa gctggatatg atttatccca aaaattagtt atttctaaaa aagcacacct
      601 cattttacct actcaccgct taatagacgc cgcacaagaa gcacaaaagg gtgatgcaaa
      661 aattggttct acgctcaaag gaattggacc tacctatgct gacaaaatta gccgaaccgg
      721 tcttcgggtg ggagatttac tacataattt taccgaaaaa tatgcaaaaa aaatagctga
      781 ccatacgcgt ttgctcgacc aaatggggtt ctcttattcg ttagatgggc tagaggacga
      841 atggatggag gcaacaaaaa ccctccttcg ttttcagtta attgatagcg aacatgttgt
      901 aaacgaatta atcgctgata ataaacgtat tttagctgaa ggtgcacaag gcacgatgct
      961 tgatattgaa tttggttcgt atccttttgt aacatcatca aacactgtgt cagctggagt
     1021 ttgtacagga ctaggcgttc ctcctaccca aattggagaa gtatatggca ttatgaaagc
     1081 atattgtacg cgcgtgggta gtggaccttt ccctacggaa ttgtttaacg aagagggaga
     1141 agaaatgcgt aaaaaaggga atgagtttgg ttcaactacg ggcagagctc gccgttgtgg
     1201 ctggatagat ttagtagcac tgaaatacgc tattatgatt aatggtgtaa cgcaattaat
     1261 tatgatgaaa agcgatgtgc ttgaccaatt tgacgtaata aaagcatgtg tgggctataa
     1321 aattgatggc gaagaatatg attatttccc ttacgaaata aacgccaact tagaacctat
     1381 gtatgtagaa ttgccaggat ggaaaacgga tatgtcaaaa atgcagaaag agagtcaatt
     1441 tccacaagcg tttaatgatt atctcgattt cttagaagac gaattggaag tacccattcg
     1501 cattgtatcg gtaggacccg atagagaaca aacaatttta cgttacatta acgaatagcg
     1561 gggtgttttt aacactgaca aacgcaaaat tggcagtatt tttgatgtat gtaattgtgt
     1621 ataaccatta acgtaatttt gtatgataat ttatgtaatt tttggagtag tagccctgag
     1681 tagttggctt atccaatacc gtttgaaatc aaagtttgaa aaatactcta aacttgcttt
     1741 aggcaatggt atgagtggaa aagaagtagc agaaaaaatg cttcgggata atggcatcag
     1801 cgatgtaaca gttatttcta caagcggaca tttaacagac cattacaacc ccactaataa
     1861 gacagtcaac ttaagtgaag cagtatacaa ctctgccagt
//
LOCUS       DAOSMY010000307         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_61126_length_1893_cov_3.372144, whole genome shotgun sequence.
ACCESSION   DAOSMY010000307 DAOSMY010000000
VERSION     DAOSMY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61126_length_1893_cov_3.372144"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLD76_06485"
     CDS             <1..985
                     /locus_tag="PLD76_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU27681.1"
                     /translation="MTCVPRMLEKIFDKLESAGRKLPFFQQKLYFWAINVAFDYNIEN
                     RTLWYTVKHKIADKLIYSKWRQAIGGNFDIVVSGGAAIQKRLASFFSAIGMPVFEGYG
                     LTETSPVIAVSSRAKYGRCAGTVGFPLEGVEVKILPVTNEIVCRGHNVMLGYYKDEAL
                     TKEVIDEDGWFHTGDTGKFTEKGQLVITGRLKNIFKTSFGKYVNSFLIEEKFVQSPFI
                     ENIVVVGENQKFAAAIIIPDFTFLKLWCERYHIPYSTNEEMLQNEDVKNRFKKEIKKY
                     NSHFGDYEQIKKFELIADEWTQQNGILTPTLKVKRSIILERYKPIIDKLFL"
     gene            1153..1743
                     /locus_tag="PLD76_06490"
     CDS             1153..1743
                     /locus_tag="PLD76_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU27682.1"
                     /translation="MKTLKKLTDDELVGLYVSGNNLAFEVLLLRHKNRVYAYIYNMVR
                     DHDMSDDIFQETFIKVITCIRTESYTANGKFCSWVTRIAHNLCMDTLRYNQKNNTVSA
                     DNTEFNVLNSISLCDKNVEDNLIEEQTFNEIERLVNMLPTNQQEIIKMRFYNDCSFKD
                     IAKKTGVSINTSLGRMRYALLNMRKLAEENNITLSF"
BASE COUNT          716 a          274 c          320 g          583 t
ORIGIN      
        1 gatgacttgc gttcctcgta tgttggagaa aatatttgac aaactcgaaa gtgctggacg
       61 taaattacct tttttccaac aaaaactata tttctgggca ataaatgttg cctttgatta
      121 caatattgaa aatcgtacac tttggtatac agtaaaacac aaaattgctg acaaattaat
      181 ttatagcaaa tggagacaag ccattggagg aaactttgac attgtagtgt ctggaggagc
      241 tgccatacaa aaacgattgg caagtttttt ttcagctata ggtatgcccg tatttgaagg
      301 ttatggatta acagaaacct ctccggtaat agcagtaagt tcgagagcaa aatatggcag
      361 atgtgctgga acagtaggat ttccgttaga aggagtagaa gtaaaaatac tccctgtaac
      421 aaatgaaatt gtatgtagag gacacaacgt tatgctcggt tattataaag atgaagctct
      481 tacaaaagaa gtaattgacg aagatggttg gtttcatact ggagatacag gtaaatttac
      541 agaaaaaggg caattggtaa ttactggaag attaaaaaac atctttaaaa cctcttttgg
      601 taaatatgta aactcctttt taatagaaga aaaatttgta caatctccct ttatagaaaa
      661 tattgtcgta gtaggcgaaa atcaaaaatt tgctgctgcc attattattc ccgattttac
      721 atttttaaaa ttatggtgcg aaaggtacca tatcccctat tctactaatg aagaaatgct
      781 tcaaaatgaa gatgtaaaaa atagatttaa aaaagaaata aaaaaataca actctcattt
      841 tggtgactat gaacaaatta aaaaatttga acttattgca gatgagtgga ctcaacaaaa
      901 tggcatttta actcctacac taaaggtaaa acgttccatt attcttgaaa gatataaacc
      961 gattattgat aaattatttt tataaaggaa taattgtatt acataacaag atacttattg
     1021 tgtaggaaat tatgttaaat attgaataaa attttgttgt attaaatttt attattttat
     1081 ttgcagttgt ataatcctta aaatcgaaag cctattgccc tattttactc tagaacacta
     1141 aaataaagag taatgaaaac actaaagaaa cttactgacg atgagttggt tgggttgtat
     1201 gtaagtggca ataacctagc tttcgaggta cttttacttc gtcacaaaaa tagagtttac
     1261 gcttatatat acaatatggt gcgtgaccat gatatgagtg acgacatttt tcaggaaact
     1321 tttattaaag taattacttg tatacgtacg gaaagttata cagcaaacgg taaattctgt
     1381 agctgggtta ctcgtatagc ccacaacttg tgtatggata cactacgtta caatcaaaaa
     1441 aacaataccg tatcagcaga taatacagaa tttaacgtat taaattctat ctctctttgt
     1501 gataaaaatg tagaagacaa cctaattgag gagcaaacat ttaacgaaat agaacgcctt
     1561 gttaatatgc tacctactaa tcaacaagaa ataatcaaaa tgcgttttta caacgattgt
     1621 agttttaaag atattgctaa aaaaacaggt gtaagcatca atacgtcatt aggtagaatg
     1681 aggtatgcac tacttaatat gcgaaaatta gctgaagaaa acaatattac actttcattt
     1741 tgaaaaatta agattattct gtacaataaa ctagagattg gtacgttcta agattatgaa
     1801 caatctaaaa tgcgtgccta tgaatcattt tacctcccgt ctacacaaaa aagaagtatc
     1861 tcaagaaaac aaccttatgc aacccaaggc aga
//
LOCUS       DAOSMY010000308         1890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_61270_length_1890_cov_2.680109, whole genome shotgun sequence.
ACCESSION   DAOSMY010000308 DAOSMY010000000
VERSION     DAOSMY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61270_length_1890_cov_2.680109"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="PLD76_06495"
     CDS             <1..1651
                     /locus_tag="PLD76_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOU27683.1"
                     /translation="DAKVYIALNTLIKDDELQEITDLIHSLYAINADALIIQDMGILE
                     LDIPPIALHASTQTDNRTLAKVAFLESVGFDQIVLARELSLQQIKHIAQNSSSKIEVF
                     VHGSLCVSYSGQCYISEALHGRSANRGECAQLCRLPYDLLDANKKIIAKDKHLLSLKD
                     LNLSDHLEELIDAGVYSFKIEGRLKDTAYVKNITAYYRQKIDAIIAKNNNLKRSSSGI
                     STYTFEPKPEKTFQRSTTSYFFHKPTESVFSVLTPKSTGEYIGTIIDIQKDFFVVDTT
                     ISIANGDGFCYISTKQLLEVFRVNKAIGNRIYPSEMLQMPIGTKIYRNADFAFSKLLH
                     AKSANRKIAISISIQETNVGYLIEFCDENMNKACREIHIDNNKAQKDQQPNIEQQLSK
                     LGNTPFYCKHISIIFTDNKFIPSSVLTQIKQTLSQELLLHRQVRYKPTRAVKTNSASL
                     FPEKELSYTGNVSNNLAHKFYTKHGVIKIDKAFELEKRLPNPVLMFCKACIKDSIGFC
                     PKKNATPFPYKEPLFLKYKEQEFLLSFNCSTCEMTIVQEKI"
BASE COUNT          718 a          313 c          284 g          575 t
ORIGIN      
        1 tgatgcaaaa gtatatattg cactaaatac attaattaag gatgatgagc tgcaagaaat
       61 tacagatcta attcactctt tatatgcaat taatgcagat gcacttatta tacaagatat
      121 gggcattttg gaacttgaca taccgcctat tgcgttgcat gctagtactc aaacagataa
      181 taggacatta gctaaagttg catttttaga gtctgtagga ttcgaccaaa tagtattggc
      241 acgagaatta tcgttacaac aaatcaaaca tattgctcaa aatagctcta gtaaaataga
      301 agtatttgta catggctcat tatgtgtaag ctacagcgga caatgctata taagcgaagc
      361 tttgcatggg cgaagtgcca atcgaggtga gtgtgctcaa ttatgtaggc ttccatacga
      421 cttattagat gctaataaaa aaataattgc aaaagataag catcttttat cgcttaaaga
      481 tttaaatttg tctgatcacc ttgaagaact aattgacgct ggagtttatt cgtttaaaat
      541 agagggacga ttaaaagata ctgcatatgt taaaaatatt acggcttact acagacaaaa
      601 aatagatgca attattgcaa aaaataataa tctaaaacgc tcatcatctg gcatatcaac
      661 ctacacattt gaacctaaac cagagaaaac cttccaacga agtactacat cctatttttt
      721 tcacaaacct actgaatcag tattttcagt tttaactcca aaatcaactg gggaatatat
      781 tggtaccata attgatatac agaaagattt ttttgtagtt gataccacta tatccatagc
      841 aaatggtgat ggattttgct atatatctac aaaacaacta ctggaagtat tcagagtaaa
      901 caaagctatt ggaaaccgca tttatccatc tgaaatgcta cagatgccta ttggaaccaa
      961 aatatataga aatgctgatt ttgctttttc aaaattactc catgcaaaat cagcaaatag
     1021 aaaaatagct atttctatct caatacaaga aacaaatgtg gggtatttaa ttgagttttg
     1081 tgatgaaaat atgaataaag catgtaggga aatacatatt gataacaaca aagcacaaaa
     1141 agatcagcaa ccaaatattg aacaacaact ttcaaaatta ggaaatacac cattttattg
     1201 caaacatatc tccattatat ttacggataa taaatttatt ccttcttctg tattaacaca
     1261 gataaaacaa actctttcac aagaattact tttacataga caagtaagat ataagccaac
     1321 aagggcagta aaaacaaact cggcatcctt attccctgaa aaagaattaa gttatacagg
     1381 caacgtaagc aataacctag cacataaatt ttataccaaa catggggtta taaaaataga
     1441 caaagccttt gaacttgaaa aaaggctacc caaccccgta cttatgtttt gtaaagcatg
     1501 tatcaaagat tcaataggat tctgtcccaa aaaaaacgca actcccttcc cttataaaga
     1561 acctttattt ttaaaatata aagagcaaga gtttctgtta tcatttaact gctcaacatg
     1621 tgaaatgact attgttcaag aaaaaatata attttttttg gaataataca attttcattc
     1681 tatatttgta agttacttaa tacctactac ttatgagaaa attgtacagt aaattatttg
     1741 ccttgatgct agtaattagt ggatttctcg ttccaatgca actacaagct gatgttcata
     1801 aaagagtcgt attgcaagca ttttggtgga actattggaa tgaaaactac ccaaatagtt
     1861 atgcaaacta ccttactgag ttagctccta
//
LOCUS       DAOSMY010000309         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_61983_length_1875_cov_2.955495, whole genome shotgun sequence.
ACCESSION   DAOSMY010000309 DAOSMY010000000
VERSION     DAOSMY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61983_length_1875_cov_2.955495"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /gene="nrdD"
                     /locus_tag="PLD76_06500"
     CDS             <1..1559
                     /gene="nrdD"
                     /locus_tag="PLD76_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOU27684.1"
                     /translation="NYCASITMYPWLIGGTNSIGGNSKAPTNLKSFSGGFINMVFMVS
                     SMLSGACATPEFLMYMNYFIQLEFGDDYYLEADKVVDLSKKQRTLDKVITDYFEQIVY
                     SINQPTGARNFQAVFWNISYYDRYYFESLFEHFVFPNGSKPHWESLNWLQKRFMKWFN
                     QERTKTVLTFPVETMAILSDNGDVKDKEYGDFAAEMYAEGHSFFTYMSDNADSLSSCC
                     RLRNEIQDNGFSYTLGAGGVSTGSKSVLTINLNRCIQHATRKGMHYLFFLEEVVDLVH
                     KVQVAYNENLKYMHEKGMLPLFDAGYINMDRQYLTIGVNGLVEAAEALDIEISDNPHY
                     LEFVQQVLGLIESYNKKYKTKGMMFNCEMIPAENVGVKHAKWDKRSGYKVNRDCYNSY
                     FYIVEDESLNIIDKFRLHGKKYIEHLTGGSALHMNLEEHLSKIQYQHLLRVAIQEGCN
                     YFTFNIPNTVCNVCGHIDKNNLKTCPKCNSHDIDYLTRIIGYMKRVSNFSQSRQLEAN
                     KRHYATISQL"
     gene            1537..>1875
                     /locus_tag="PLD76_06505"
     CDS             1537..>1875
                     /locus_tag="PLD76_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOU27685.1"
                     /translation="MLRYLNYNIVFQEVPDEVTLAIHISQCPHRCKGCHSPILQQNSG
                     EYLNESVLDHLLHTYQNAITCICFMGGDVDPKELERLALYVKQHSNIQTAWYSGSVKI
                     PEFCNILAFDY"
BASE COUNT          636 a          326 c          366 g          547 t
ORIGIN      
        1 ccaattattg tgccagcatt accatgtatc cttggctcat tggtggtaca aactcaattg
       61 gaggaaactc taaagccccc accaacttaa aatcctttag cggaggtttt atcaatatgg
      121 tgtttatggt atcgagcatg ctgagtggag cttgtgccac tcccgaattt ttgatgtaca
      181 tgaattattt tattcaacta gaatttggcg atgattatta ccttgaggca gacaaggtgg
      241 ttgacttgtc gaaaaagcaa cgtactttgg ataaggtaat taccgattat tttgagcaaa
      301 ttgtgtactc tatcaatcaa cctactggtg ccagaaactt tcaagctgta ttttggaata
      361 tttcgtatta cgaccgttac tattttgaaa gtttatttga acattttgta tttccaaacg
      421 gtagtaaacc acattgggaa tcattaaatt ggttacaaaa gcggtttatg aaatggttta
      481 atcaagaacg cacaaaaaca gttttgactt ttcccgtaga aacgatggct atactaagcg
      541 ataatggcga tgtaaaagac aaagaatatg gagattttgc agcagagatg tatgcagaag
      601 gacactcctt ttttacctat atgagcgata atgctgactc gttaagcagt tgctgtaggt
      661 tacgcaacga aattcaggac aatggattta gctatacctt aggtgctggt ggtgtttcta
      721 cgggatcgaa aagtgtactt actattaatc taaataggtg tattcaacac gctacacgca
      781 agggaatgca ttacttattt ttccttgaag aagtggtaga tttagtacac aaagtacaag
      841 tggcatacaa cgaaaacctg aaatacatgc acgaaaaagg gatgttaccc ttgtttgatg
      901 ctggatacat caacatggat cgtcagtacc taaccatagg tgtgaatgga ctggtagaag
      961 ctgccgaagc tttggatata gaaatatccg acaacccaca ttacctagaa tttgtgcaac
     1021 aagtattggg actaatagaa agctacaaca aaaagtacaa aacgaaaggc atgatgttta
     1081 attgcgaaat gatacctgcc gaaaatgtag gtgtaaaaca tgctaaatgg gacaaacgaa
     1141 gtggttataa agtaaataga gattgttaca acagctattt ctacattgta gaagatgagt
     1201 cgctcaatat tatagataaa ttccgtttgc atggtaaaaa atacattgaa cacctaacag
     1261 gaggctccgc actacatatg aatttagaag aacacctaag taaaattcaa taccagcatt
     1321 tgcttcgtgt agccattcaa gaaggatgta attattttac cttcaatatt ccaaatactg
     1381 tgtgcaatgt atgtgggcat attgacaaaa acaacctaaa aacttgtcca aaatgcaaca
     1441 gtcatgatat tgattaccta acacgcatta ttggatacat gaaacgagta agtaattttt
     1501 cgcaaagcag acaattagaa gcaaataaac gccattatgc tacgatatct caattataac
     1561 attgtttttc aagaagtgcc tgatgaagtt acattggcta ttcatattag tcaatgtcct
     1621 catcggtgca agggatgcca tagccctatc ctacaacaaa atagtgggga atatttaaac
     1681 gaaagtgtgc ttgaccacct actccatacg tatcaaaacg ccattacttg tatctgtttt
     1741 atgggtggag acgtcgatcc aaaagaatta gaaagacttg ccctatacgt aaaacaacac
     1801 agtaatatac aaactgcatg gtactcagga agcgtaaaaa ttcctgagtt ttgtaacatc
     1861 cttgcttttg attat
//
LOCUS       DAOSMY010000310         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_61985_length_1875_cov_2.931319, whole genome shotgun sequence.
ACCESSION   DAOSMY010000310 DAOSMY010000000
VERSION     DAOSMY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61985_length_1875_cov_2.931319"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1351)
                     /locus_tag="PLD76_06510"
     CDS             complement(206..1351)
                     /locus_tag="PLD76_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU27686.1"
                     /translation="MKNIYFVIIISVISVIYLLLNAYVYHRANVALPNKMARNTFTVL
                     FWLCSVSFLIGQFLERTPWVNLTTVISGIGSVWLALFFYALLFVLVIDIFALLHRLTS
                     LLPVSFLEIATPFRLFVVVAVSTIFLVTAGYINARYPTINEYEVHINKQVEGKKHLTI
                     ALATDIHMGYIIGNKRLSDLVSSLNAIEADVIIFAGDMIDHNMVPVKYHTMGEILSQL
                     QAPLGVWAVTGNHEYIGNAESAVDYLSRYGIQFVRDTVVNVQGIQLVGREDAQKINFT
                     QVNRKSLETIFEQVDSKLPIIVIDHQPLEQDRVVALGADLMVSGHTHQGQLWPFKILT
                     KLIYKIEDGLVQKGNTHLYVSPGYGTWGPPVRIGNRPEIAVFRLVFD"
     gene            complement(1456..>1875)
                     /locus_tag="PLD76_06515"
     CDS             complement(1456..>1875)
                     /locus_tag="PLD76_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU27687.1"
                     /translation="LYKSLLFEVNNEDSIIEKAPETPIDTSSKPMLEKVVIKEGSTIR
                     VIPLQDIVYLQAEGDYVMIRTLDGSFIKEQTMKSFEQQLPPQQFVRVHRSYIVALAAL
                     KGVELYEKQQYLLHLKNGEKIKMSINGYKLLKQQLAL"
BASE COUNT          588 a          377 c          310 g          600 t
ORIGIN      
        1 aaccttactt aacttattca tatttggcta ttatttgggt gattgtttct tttttcagcg
       61 acaaaaatag atataataaa cacaacaaac aagaagataa gagctccccc cccccgatgt
      121 ttaactttta ctaactatct cgttagcgag tttgaaacat tccctgtaat aaagcctaat
      181 catagtatat attaatggta aattatcaat caaaaaccaa tctaaacaca gcaatttcgg
      241 gtctgttgcc tatgcgtaca ggaggacccc aagtgccgta tcctggcgaa acatacaaat
      301 gggtattgcc tttttgtact aacccatctt caattttata aataagttta gtcagaattt
      361 taaagggcca aagttgcccc tgatgggtat gtcctgaaac catcaaatca gcccccaaag
      421 ccactactct atcctgctct aaaggttggt ggtcaattac tatgatgggc aacttgctat
      481 ctacttgttc aaagatggtt tctagcgatt ttctgttgac ttgcgtaaaa ttaatcttct
      541 gagcatcttc cctgcctacc agttgaatgc cctgcacatt gactacagta tcgcgtacaa
      601 actgaatgcc ataacgagac aaataatcta cagcactctc tgcattgcca atatattcgt
      661 gattgcctgt aacagcccat acgcccaaag gtgcttgtag ttggctcaat atctctccca
      721 tagtgtggta tttaacaggc accatattat gatcaatcat atctcctgca aaaataatca
      781 catcggcttc aatcgcatta agtgaggaga ctaaatcact aagcctcttg ttaccaataa
      841 tatagcccat atgtatatcc gtagccaaag caatggtgag gtgttttttc ccttctactt
      901 gtttgtttat atgcacttcg tattcattga tagtgggata ccgtgcattg atatatcctg
      961 cggttactaa aaaaatggtg cttacggcaa ctaccacaaa caaccgaaat ggtgtggcta
     1021 tttctaaaaa cgatacaggc aatagcgatg taagcctgtg tagcaaggca aatatatcaa
     1081 tcacaagtac gaacaacaag gcataaaaaa acaaagcaag ccatacagaa cctatgccac
     1141 taatcaccgt agtgagattg acccaaggtg tacgttctaa aaactgaccg attaaaaacg
     1201 atacgctaca caaccaaaat aacaccgtaa aagtatttct cgccatttta ttgggtaaag
     1261 ctacattagc cctgtgatac acgtaagcat ttagtaataa ataaattaca ctaattaccg
     1321 atatgataat gacaaaatag atatttttca tccgtaaata atttttagtt taaggttgga
     1381 tggaactcca tgtttgctat tttcattggt gtttgtgttt tttcgcaaac atgttcgttt
     1441 cataattggt acttattaga gtgcaagttg ctgttttaat aatttgtatc cattgatact
     1501 cattttaatt ttctctccat tttttagatg cagcaaatat tgttgttttt catacaattc
     1561 aacccctttt aaagctgcta aagctactat atacgaacga tgtacacgca caaattgttg
     1621 tgggggtaat tgttgttcaa aagatttcat ggtttgctct ttgataaaac tcccatcaag
     1681 agttcttatc attacataat cgccttcagc ttgcaaataa acaatatctt gaagcggaat
     1741 aacacgaatt gtgctacctt cttttatcac aactttttca agcataggtt ttgagctagt
     1801 gtcaatggga gtttcgggtg ctttttcgat aatcgaatct tcgttgttta cctcaaaaag
     1861 taagctttta tacaa
//
LOCUS       DAOSMY010000311         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_62256_length_1869_cov_2.628997, whole genome shotgun sequence.
ACCESSION   DAOSMY010000311 DAOSMY010000000
VERSION     DAOSMY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62256_length_1869_cov_2.628997"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1280)
                     /locus_tag="PLD76_06520"
     CDS             complement(42..1280)
                     /locus_tag="PLD76_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU27688.1"
                     /translation="MKKTLWMTLGLLLFTIATGCAQKSQVNKAYNRLFTEPIDYAAAT
                     EDIEVAKKDSTTATQSRTWYVAGRIGYTMANTEVTKMRMQQPANDENLYQGLKQMYDN
                     YVVADKFDGVVDKKGRIKYSQRRNIKADFKEMHPFYINAGATMFEYKEFAKAYTLFNQ
                     YIKIADLAIWEEKDAIKIDSTYNTIQFYAGIVASNMDSTQLAIKHFNNLIGTSYPQKE
                     AVYEMLYSLYQHANDTVNFISILKEGVELYPESPFFIGNLINYYATASKFTEALDYLN
                     EALERSPKNIEYLNVKGTLLIQMGEFDKARAIYTESLALNNQHPNSLFGMGRSWAFEG
                     DLINEKSQDIKDNKLYKKEQERAKEYFINSIKYLEEAKALMSKEDAQYSDLLQNLRVL
                     YLRLGETEKYNKIDAEIKTY"
     gene            complement(1300..>1869)
                     /locus_tag="PLD76_06525"
     CDS             complement(1300..>1869)
                     /locus_tag="PLD76_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOU27689.1"
                     /translation="EMGRTATGVRGITLDEDGQDEVIGMITIPKEEKTKESILVVSEK
                     GYGKRTLIDEIDENGNAIVDENGDPVPVYRITNRGGKGVKTMNITDKTGKLVAIKNVS
                     DNNDLVIINKSGIAIRLEVASMRIMGRATQGVRLINLEKRNDEIASVCKVVSEEKENL
                     DEVSPELLDAENNFNSIEGIATNDETNNE"
BASE COUNT          557 a          367 c          238 g          707 t
ORIGIN      
        1 cgaaaagcct cacaataata aaacaggagt atcacaacat tttagtaagt tttgatttct
       61 gcatctattt tgttgtattt ttctgtctca cctaaacgca aatacaatac acgcaaattt
      121 tgcaataaat cgctatattg tgcatcttct ttagacatca atgcttttgc ttcctccaaa
      181 tatttaattg aattaataaa atattctttg gctctttctt gttctttttt atacaactta
      241 ttatccttaa tatcttgcga tttctcatta attaaatctc cttcaaaagc ccatgatcta
      301 cccattccaa acaacgaatt tggatgctga ttatttagtg ctagtgattc agtataaata
      361 gctcttgctt tatcaaactc acccatttgt atcaacaaag tacctttcac attaaggtat
      421 tcaatatttt taggagaacg ttccaaggct tcatttaaat agtctaatgc ttctgtaaat
      481 ttacttgctg tagcataata attaattaaa ttacctataa aaaaaggact ttctggatac
      541 aattcaacgc cttcttttaa tattgatata aaatttactg tatcattggc atgttgatac
      601 aatgaataaa gcatctcgta aacagcctcc ttttgtggat acgaagtacc tattaaatta
      661 ttaaagtgct taatagccag ttgagtacta tccatattag atgcaacaat ccctgcataa
      721 aactgaattg tattataagt ggagtctatt tttatcgcat ctttttcttc ccatatagct
      781 aaatcagcaa tcttaatgta ctgattaaac aatgtatacg ctttggcaaa ctctttatat
      841 tcaaacatag tagcaccagc attgatataa aaaggatgca tttctttaaa atcggctttt
      901 atatttctcc gttgagaata ttttatacga ccctttttat ctactacacc atcgaacttg
      961 tctgcaacaa cataattgtc gtacatttgt tttaatcctt gatacaaatt ttcatcgtta
     1021 gcaggttgtt gcatacgcat cttcgttact tctgtatttg ccattgtata gccaatacga
     1081 ccagcaacat accaagtacg ggattgtgta gcagttgtag aatctttttt tgcaacttca
     1141 atatcttcgg ttgctgcagc ataatcgatt ggttcagtaa acaaacgatt atacgcctta
     1201 tttacttgcg atttttgtgc acagcctgtt gctattgtaa acaataacaa acctagtgtc
     1261 atccatagtg tttttttcat aaatagattt ttttgtttat tattcattat ttgtttcatc
     1321 gttggtagca ataccttcaa tcgaattaaa gttattttct gcatctagta attctggcga
     1381 aacttcatct aagttttctt tctcttctga aacaacttta caaacagaag ctatttcatc
     1441 gttacgtttt tcgagattga ttagccttac gccttgtgta gcacgtccca taatacgcat
     1501 acttgctact tctaggcgta tagcaatacc cgatttgtta ataatcacca aatcattgtt
     1561 atcagatacg ttctttatag ctactaattt acctgtttta tcagtaatat tcatggtttt
     1621 tacaccttta cctcctctat tagttattct gtaaacaggc acaggatctc cattttcatc
     1681 tactatagca tttccatttt catcaatttc atcaatcaat gtacgtttac catatccttt
     1741 ttcagaaacc actaaaatac tttcctttgt tttttcttct ttcggaatgg taatcatacc
     1801 aattacctca tcttgtccat cttcgtcaag tgtaatacca cgaacaccag tagcagtacg
     1861 tcccatttc
//
LOCUS       DAOSMY010000312         1861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_62591_length_1861_cov_3.217608, whole genome shotgun sequence.
ACCESSION   DAOSMY010000312 DAOSMY010000000
VERSION     DAOSMY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62591_length_1861_cov_3.217608"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLD76_06530"
     CDS             complement(<1..509)
                     /locus_tag="PLD76_06530"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOU27690.1"
                     /translation="MIHVVFRYDDYVLRENSFQNSLLAVFETYNTPLICGIIPATYNA
                     STDITFLKKLYQEKKVIIAQHGYRHEDNKLNSFKSEFCGSSFGSQIVAIRGGQAVLKD
                     LGFESSIFIPPFNTADNQTYRSLYKLGYKLISTSGSYNSPYVSSLPATSTFDQLLADI
                     DLAKKQYEI"
     gene            complement(569..1699)
                     /locus_tag="PLD76_06535"
     CDS             complement(569..1699)
                     /locus_tag="PLD76_06535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU27691.1"
                     /translation="MENKFIPVCEPFLNGNELKYVTDAVETGWISSAGKYVTEFEKQF
                     AAYCGCNYGVAVCNGTVSIHVALVALGIGKGDEVLVPTFTMIASAFAVCYTGATPIFV
                     DADKDTWNIDISKIEEKITPRTKAIMPVHLFGLMCDMDAIVALAKKYNLFIIEDAAQA
                     HGAEYKGIRAGAFSDIASFSFFANKNLTTGEGGMLVCKNESYYNKLRYHKNLCFPLDG
                     NRNYTHDEIGFNYRMSNVVAAIGLAQVEKADEYKAMRMRNNNLYRTLLADVPGIIFQP
                     HLEGYVNVGWMNAIVIDADVYGRTKDEMIPLLKEKGIDTRLLFNGMHNQEALKKYTGN
                     TNMGEFPVSDWLTQNGLYLPSASNLSEDTISEICDLISQYAK"
BASE COUNT          601 a          375 c          277 g          608 t
ORIGIN      
        1 tctatctcat actgtttttt tgccaaatca atatctgcaa gcagttggtc aaacgtagaa
       61 gttgcaggca atgatgatac ataagggcta ttataagatc ccgaagtaga aattagttta
      121 tatcccaact tatataaact acggtaagtt tggttatctg ccgtattaaa aggaggaata
      181 aaaatagaag actcaaaacc taaatctttt agcaccgctt gcccccctcg aatcgcaaca
      241 atctgactac caaaagacga gccacaaaat tctgatttaa aactatttag tttgttgtct
      301 tcatgcctat atccatgttg tgcaataatc actttttttt cttgatacaa ttttttcaga
      361 aaagttatat ctgtagaggc attataagtt gctggaataa taccgcaaat caagggagta
      421 ttataagttt caaaaacagc taacaagcta ttttgaaaac tattctctct caaaacataa
      481 tcatcatatc taaaaacaac atgtatcata gagtgtaaat tgagtccaat taggctaaat
      541 ataataaata ataaaaaacg ttgcattgtt actttgcata ttgacttatc agatcacaaa
      601 tttcagaaat tgtatcttcc gacaaattac tagccgaagg caagtacagt ccattttgag
      661 ttagccaatc gctcacagga aattcgccca tgtttgtatt gcctgtatat ttttttaatg
      721 cttcttgatt gtgcatacca ttaaataata aacgtgtatc aatccctttt tctttgagca
      781 atggaatcat ctcatctttg gtacgtccat atacatcggc atcaataaca atagcattca
      841 tccaacctac atttacatat ccctctaaat gtggttgaaa aataatgcct ggtacatctg
      901 ctaacaacgt gcggtataaa ttattatttc gcatacgcat ggctttgtat tcgtccgctt
      961 tttctacttg agcaagtcca atagcagcca caacattact catacgataa ttaaatccaa
     1021 tttcatcgtg ggtataattg cgattaccat ctaatgggaa acataaattc ttgtggtaac
     1081 gtagtttgtt gtagtaactt tcgtttttac atacaagcat accaccctcg cctgtagtta
     1141 ggtttttgtt agcaaaaaaa ctaaatgagg caatatccga aaatgcccca gctctaattc
     1201 ctttgtattc agctccatgt gcttgtgcag catcttctat tataaataaa ttatattttt
     1261 ttgccaacgc tacaatcgca tccatatcgc acattagccc aaataaatgc acaggcataa
     1321 tggctttggt acgaggagtt attttttctt caatttttga aatatcaatg ttccaggtat
     1381 ctttatctgc atctacaaaa atgggcgtag cacctgtata gcaaacggca aaagcagaag
     1441 ctatcatggt aaaagtggga actaatacct catctccttt gcctataccc aaggctacta
     1501 aagctacatg gatagataca gtaccattgc atacagccac tccatagttg caaccacaat
     1561 atgcagcaaa ctgtttttca aactcggtaa catacttccc tgcagatgat atccaaccag
     1621 tttctacagc atcagttaca tattttaatt cgtttccatt taaaaatggt tcacaaacag
     1681 gtataaattt attttccatg gttttttatt ttactattac gtgcgaatca tcaacggcat
     1741 caaatcgcac tttatctaat tcgccacaat aaggaccttg ttttacttct atcatttccg
     1801 atggttctaa cattttgaat ccatgaccac cgtgagctaa taaaattaca tctccttggg
     1861 t
//
LOCUS       DAOSMY010000313         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_63101_length_1851_cov_2.248330, whole genome shotgun sequence.
ACCESSION   DAOSMY010000313 DAOSMY010000000
VERSION     DAOSMY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63101_length_1851_cov_2.248330"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..>1851
                     /locus_tag="PLD76_06540"
     CDS             206..>1851
                     /locus_tag="PLD76_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27692.1"
                     /translation="MRAQALHARDLGNDLYLAVSGVARWADEGIIEGSFYNSAGIFTA
                     LEKKFADKHSLSLTLFAAPTQRGQQGATLQEVYDLAGTIYYNPYWGYQDGQKRNSRVV
                     KTIDPTAIVSYEWKIDPQQVLRAGFGYHYNFYSNSALNFYNAPDPRPDYYRYLPSFQT
                     TQEDKDLITSLWKTNPSVSQINWDELYRANYALNTYDEEMGTESSAKYALERRHNDLE
                     ELTFNAAYVNQFTKALKLTVAIEAKNNVGKHYKTMEDLLGGKRWIDTDQFAERDFADN
                     PDIIQNDLQDPNRVIGVGDIFGYHYNMHVFNAKAFIANDWTISSLDFYYAGQLSYTQF
                     FREGFMENGRATDQGIGSYGVGDLWFFTDPSVKAGMTYHIDGRNRISYNMLGEYRAPL
                     VNNAYVSPRIKDELIPYLTQEKVLSYDLAYEFVYPTFRGRLSVFQTHVYDAVENMGYY
                     DDEYRTFVNHSLYDVNKVYRGVEAGVAIQLPYSLTLSLTGTLADYHYKDDSQGIMSAE
                     NGSDLDGGVEGDILEIVKTKDLKINAGPQTAASVKLSYVHP"
BASE COUNT          590 a          302 c          395 g          564 t
ORIGIN      
        1 aatgccttag ctcgtggaat gtttaactat tcgatgcttg gagggttaaa tgatgcgatg
       61 cgtaataagg atgtgaacag aggaatgtcc ccccacagct tattcatatg gaaatgtagc
      121 tggagttacc aatattgata ccagagccag tcagtatgca ttaggtacac gcaccagtgt
      181 tgcctttagc aaccgttctt ataccttgcg ggcacaagca ttacatgcac gtgatttggg
      241 caatgatttg tacttggctg tatctggagt ggcacgttgg gctgatgaag gaattatcga
      301 aggttcgttt tataattcag cagggatttt tactgcttta gaaaagaaat ttgcagataa
      361 acacagcctt tcactcacac tttttgctgc acctacgcaa cgagggcaac aaggagccac
      421 tttgcaagaa gtatatgatt tagcgggtac tatctattac aatccgtatt ggggctatca
      481 agatggacaa aaacgtaatt cgcgtgtagt gaaaaccatt gaccctactg ctattgtgag
      541 ctacgaatgg aagattgatc cacagcaagt tttacgtgct gggtttggat atcattataa
      601 cttttatagc aattcagctc tcaactttta caatgcacct gatccacgac cagattatta
      661 ccgctacttg cctagttttc agacaacaca agaagacaaa gatttgatta cctctttgtg
      721 gaaaacaaac cctagtgttt cgcaaattaa ttgggacgaa ttatacagag ccaattatgc
      781 tttaaatacc tatgatgaag aaatgggcac agaatcgagt gcaaaatatg cattagaaag
      841 aagacacaat gatttagaag aactaacatt taatgcagca tacgtaaatc aatttacaaa
      901 agctctaaaa cttaccgttg ctatagaagc caagaataat gttggtaaac attacaaaac
      961 gatggaagat ttgcttggtg gcaaacgttg gattgatact gatcaatttg ctgaaagaga
     1021 ttttgcagat aatccagata ttattcaaaa tgatttacaa gatccaaata gagtgattgg
     1081 agtgggagat atttttggct accattacaa tatgcatgta tttaatgcaa aagcttttat
     1141 cgccaatgat tggacaattt cttctttgga tttttattat gcaggacaat tatcgtacac
     1201 tcaatttttc cgtgaaggat ttatggaaaa tggtagagct acagatcaag gtattggttc
     1261 gtatggagtt ggggatttgt ggttttttac cgacccctct gttaaagcag gtatgaccta
     1321 tcatattgat ggtagaaatc gtatttcgta taatatgttg ggagaatata gagctccatt
     1381 ggttaacaat gcctatgtgt cgcctagaat aaaagatgaa ttaattcctt atttaactca
     1441 ggaaaaggtc ttgtcatacg accttgcgta cgaatttgtg tatccaacgt ttagaggacg
     1501 tttatccgtt tttcaaacac atgtatatga tgccgttgag aatatgggat attacgatga
     1561 tgagtatcgt acatttgtaa accattcctt gtatgatgtg aacaaagtat acagaggtgt
     1621 tgaggctgga gttgctattc agttgccata tagcttaaca ttatcattaa caggaacttt
     1681 ggctgattac cattataaag atgattctca aggaattatg agtgcagaaa atggtagtga
     1741 tttagacgga ggagttgagg gcgatattct agaaatagta aaaacaaaag acttaaaaat
     1801 taatgcaggt ccgcaaactg ctgctagtgt aaaattgagt tatgtgcatc c
//
LOCUS       DAOSMY010000314         1826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_64294_length_1826_cov_4.224167, whole genome shotgun sequence.
ACCESSION   DAOSMY010000314 DAOSMY010000000
VERSION     DAOSMY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64294_length_1826_cov_4.224167"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLD76_06545"
     CDS             complement(<1..659)
                     /locus_tag="PLD76_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOU27693.1"
                     /translation="MEYTFFDLLVLIGSLGLFLYGMKLMSEGLQRVAGDSLRSVLAAM
                     TKNRIMGVITGVLVTAIIQSSSATTVMVVSFVNAGLLTLFQSITVIMGANIGTTVTAW
                     IISLFGFKVSIAAFAVPLIAFAFPLVFSSNNKRKSLGELILGFALLFMGLDMLKNAVP
                     DLRSNPEILAFLTQYTNFGFGSVLIFLLIGTILTIVVQSSSATMAITLIMCSKGWISF
                     E"
     gene            758..>1826
                     /locus_tag="PLD76_06550"
     CDS             758..>1826
                     /locus_tag="PLD76_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU27694.1"
                     /translation="MGNYLEIICKNNQKKALIPFGRSLAEVFEDLKIELPYQVVAARV
                     NNKIESLNYCLYRNKTIEFVDLSCESGMRVYVRSLVFVLFKAIVDLIPNGRLRVEHPV
                     SKGYYCEITANGEKISFEQLAAIKIRMKEIIEQNIRFVSEDKPTSEVIEVFAKHNRTD
                     NVDLLQTYKNYYTEYHSLDGLPDFYSGVVLPSTGYLSLFDIHPYYKGFLIQIPKRSNP
                     IELEEIVVQNKLLEVFADHYKWCRIMGINNIADFNNIEKGKLRDFIKVTEALHEKQIS
                     KMADTIAERSEKTKIVLVSGPSSSGKTTFTKRLSVQLMASGIKPISLSLDNYFVDREN
                     TPLDEKGDYDFESLYALDIAYF"
BASE COUNT          667 a          309 c          315 g          535 t
ORIGIN      
        1 aattcaaatg atatccaacc tttactacac ataatcagcg taattgccat agtagcagaa
       61 gaagactgca ctacgatagt taatattgtt cctatgagca agaatatcaa cacagaacca
      121 aacccaaagt tggtatattg ggttaagaac gccaaaattt ctggattact tcgtaaatca
      181 ggaacggcat ttttcaacat atccaatccc ataaacaaaa gggcaaaacc caagatcagc
      241 tctcctaagg attttctttt gttattagaa gaaaatacca atggaaatgc aaaagcaatc
      301 agtggaacgg caaatgcagc aatactaacc ttaaaaccaa acagagaaat tatccaagcg
      361 gtaacagttg tacctatatt ggctcccatg ataacggtga ttgattggaa aagcgttaat
      421 aatcctgcgt taacaaaact tacaaccatc actgtggttg cagaagacga ctggatgata
      481 gccgtaacta aaacgcctgt aataaccccc atgatacggt ttttggtcat tgctgccaat
      541 actgaacgta agctatcgcc tgctactcgt tgcaaacctt cactcataag cttcattcca
      601 tacaaaaaca agcctaaaga gcctattaat actaataaat caaaaaatgt gtattccata
      661 acggatttta tttaaaaaag taaggtacaa aaataatatt ttttatacat ttacaaagat
      721 tttttaaata aaagttaaat aaatatagca tacagttatg ggaaactacc ttgagattat
      781 ctgtaagaac aaccaaaaga aagctttaat tccttttggt agaagcttag cagaagtatt
      841 tgaagattta aaaattgaac taccttatca agtggtagcc gctcgagtaa ataataaaat
      901 tgaaagctta aattattgcc tttatcgcaa taaaactatc gaatttgtag atttgagttg
      961 cgaatcgggc atgcgtgtat atgtacgttc gttggtgttt gtattgttta aagccattgt
     1021 agacttaatt cccaatggga ggcttcgggt agaacatcca gtatcgaagg gatattattg
     1081 cgaaattacg gctaatggag aaaaaatctc ttttgaacaa ttagctgcta ttaaaatacg
     1141 tatgaaagaa atcattgagc aaaacatccg atttgtatca gaagacaaac caacatcgga
     1201 ggtaattgaa gtttttgcaa aacacaacag aacagataat gttgatttgt tgcaaacata
     1261 taaaaactat tacacagagt accattcgct tgatggatta cccgattttt acagtggcgt
     1321 agtattaccc agcactggtt atttatctct atttgatata catccgtatt ataaaggctt
     1381 tttgatacaa atacccaaac gtagtaaccc aatagaactt gaagagattg tggtgcaaaa
     1441 taaattatta gaagtttttg ctgatcatta taaatggtgc agaataatgg gcatcaataa
     1501 tatagctgat ttcaataaca tagaaaaagg caagctacgt gattttatta aagtaactga
     1561 agctttacac gagaagcaaa tttcaaaaat ggcagatact atcgccgaac ggagtgaaaa
     1621 aacaaaaatt gtattggttt caggtccttc ttcttctggt aaaactacct ttaccaaacg
     1681 attgagtgtg caacttatgg cgtcgggtat taaaccaatt tctttatcat tagacaatta
     1741 ttttgtagat agagaaaata ccccattaga cgaaaagggc gattatgatt ttgaatcttt
     1801 gtatgcactc gacatcgctt atttta
//
LOCUS       DAOSMY010000315         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_64599_length_1820_cov_2.308782, whole genome shotgun sequence.
ACCESSION   DAOSMY010000315 DAOSMY010000000
VERSION     DAOSMY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64599_length_1820_cov_2.308782"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLD76_06555"
     CDS             complement(<1..912)
                     /locus_tag="PLD76_06555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861786.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOU27695.1"
                     /translation="MMASIKASEVSNLLQMELEGFSDKLKFEETGKVLQISDGVARVF
                     GLTKVQSNELVLFENGTKGVVLNLEEDNVGIVLLGSTRGIKEGDTVQRTGNIASILAG
                     EGLLGRVVNTLGEPIDGKGEIKGELFEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKTEIAIDTIINQRSNFLAGDPIYCIYVGIGQKGSTIAGIVK
                     TLEEYGAMDYTIVVSATASDPAAMQFYAPYAGVAIAEYFRDTGRHALIIYDDLSKQAV
                     SYREVSLVLRRPPGREAYPGDIFYLHSR"
     gene            complement(924..1460)
                     /gene="atpH"
                     /locus_tag="PLD76_06560"
     CDS             complement(924..1460)
                     /gene="atpH"
                     /locus_tag="PLD76_06560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOU27696.1"
                     /translation="MNEGKISVRYARAILNLAKNKGIEDAVYKEMFVLSDNLTHYAKD
                     FKTAFAHPDITEENKKKLFKLAVGNTISSFTSQSIDFIVEKKRSSFMLRIALMYQTLY
                     QIEHNIIISNVISAKELDKKTLDKIKAFASKTFDASVELVSTIDPDLIGGFILDIANQ
                     RLDASIKGKLNNIRKIVY"
     gene            complement(1469..>1820)
                     /locus_tag="PLD76_06565"
     CDS             complement(1469..>1820)
                     /locus_tag="PLD76_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOU27697.1"
                     /translation="ANEAQQKLLEIEDKGKEIIANAEQQQLLIVKESQELKKKLVEEA
                     KMQAQKEADNIIENARIQIEKEKETALSDIRNEVLQVSFSIAEKVLEKELENKKAHTQ
                     YVEKLLDNMSMSQS"
BASE COUNT          500 a          358 c          272 g          690 t
ORIGIN      
        1 acgtgaatgc aaataaaaga tatcaccagg atatgcttca cgaccaggtg gacgacgaag
       61 taccaacgat acttcacgat aagaaactgc ttgttttgat aaatcatcat aaataattaa
      121 tgcatgacga cctgtatctc taaaatattc agcaatggca actcctgcat agggagcata
      181 aaattgcatt gcagcagggt cagaggctgt tgcagatacc actatggtat aatccatggc
      241 accatactct tcgagggttt ttacaatgcc agctatggtc gacccttttt gtcctatacc
      301 cacataaata cagtaaatag gatctcctgc taaaaagtta ctacgttgat tgattatcgt
      361 atcaatggct atttctgttt tacccgtttg tctgtctcca atgattaact ctctctgccc
      421 acgtccaatc ggaatcatag catcaatggc tttaattcct gtttgtagtg gttcgtttac
      481 gggttgtcta aatattacac ctggagcttt cctttccaac ggcatttcaa ataattcgcc
      541 tttgatttcg ccttttccat ctataggttc gcccaatgta tttactacac gccctaatag
      601 tccttcgcca gctaagatag aagcaatatt tcctgttctt tgtacagtgt ctccttcctt
      661 aattccacgg gtagatccca ataaaacaat acctacattg tcttcttcca gatttaaaac
      721 tactcctttt gtgccatttt caaaaagtac aagctcattc gattgcacct tagttaatcc
      781 aaaaaccctt gctacaccgt cacttatttg taacaccttg cctgtctctt caaattttag
      841 tttatcacta aaaccttcta gttccatttg tagcagattc gacacttcac ttgcttttat
      901 gcttgccata attggatata tttttagtat actatttttc taatattgtt taattttcct
      961 ttaatactcg catctagccg ttggttggca atatctaaaa taaaccctcc tattaagtcg
     1021 ggatctattg tactgactag ttctacagat gcatcaaacg ttttacttgc aaacgctttt
     1081 attttatcta atgttttttt gtctaattct ttagcactta ttacattgct aatgataata
     1141 ttgtgttcta tttgatataa agtttgatac attaatgcaa tgcgtagcat aaatgagctt
     1201 cttttttttt ccacaataaa atcaatagat tgtgatgtaa atgatgagat ggtattacca
     1261 acagcaagtt taaacaattt ttttttgttt tcttcggtta tgtcaggatg ggcaaaagcg
     1321 gttttaaaat cttttgcgta atgagttaag ttgtccgaaa gtacaaacat ttctttgtac
     1381 acagcatctt caataccttt atttttagct aaattaagga ttgctctagc atatctaact
     1441 gatattttac cttcgttcat gtataattct atgattgaga cattgacata ttatctaata
     1501 acttttctac atactgggta tgagcttttt tattttcgag ttctttttca agtacctttt
     1561 ctgcaataga aaaggatacc tgaagtactt cattacgtat atcagataaa gctgtttctt
     1621 tttctttttc aatttggatg cgagcatttt ctataatatt atcagcttcc ttttgtgctt
     1681 gcattttagc ctcttcaact aatttctttt ttaactcttg agattcttta actatcaaga
     1741 gttgttgttg ttcagcattg gcaataattt ctttcccttt gtcttctatt tctaggagtt
     1801 tttgttgagc ctcattagcc
//
LOCUS       DAOSMY010000316         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_64714_length_1818_cov_2.048213, whole genome shotgun sequence.
ACCESSION   DAOSMY010000316 DAOSMY010000000
VERSION     DAOSMY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64714_length_1818_cov_2.048213"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLD76_06570"
     CDS             <1..268
                     /locus_tag="PLD76_06570"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU27698.1"
                     /translation="IVALESAVLFESGFDKYIDKSIVVTAPLQVRINRIMKRDKLTYP
                     EVEARIKNQLSDEEREARADFVIKNDGVEHLPSQLFAFLKAVDF"
     gene            complement(265..816)
                     /gene="def"
                     /locus_tag="PLD76_06575"
     CDS             complement(265..816)
                     /gene="def"
                     /locus_tag="PLD76_06575"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU27699.1"
                     /translation="MIRPIVVYGNAVLRKKADPIDKNYPELSSLINDMFDTLSHAEGV
                     GLAAPQVGLPIRLFIVDLSILGDDEPSFVNFKKVMINPLITSFEGEELSQEEGCLSIP
                     GISETVKRSDKISIEYYDENWQKHVEQYSGFPSRVMQHEYDHIEGHVFVDKISPIRRQ
                     MISSKLNALTKGKFNCHYKVQKV"
     gene            complement(813..1238)
                     /gene="ruvX"
                     /locus_tag="PLD76_06580"
     CDS             complement(813..1238)
                     /gene="ruvX"
                     /locus_tag="PLD76_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOU27700.1"
                     /translation="MGRIIAIDYGKKRVGIAVTDTLQLIANGLTTVASHEVIAFLEHY
                     IQTEPVDKIIIGLPKQMNNELSESMKYITPFVHTLKKRITHIPIEYVDERFTSVLAHR
                     TMLEAGLKKKDRQNKALVDTISATIILQTYLESIKTNTL"
     gene            1386..>1818
                     /locus_tag="PLD76_06585"
     CDS             1386..>1818
                     /locus_tag="PLD76_06585"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOU27701.1"
                     /translation="MTKIIVTGGTGYIGSHTVVELQQAGYEVVIIDNLSNSSVDVLQG
                     IFAITGVMPSFEKIDCTDYQAMESVFKKHADAQAIIHFAASKAVNESVEKPLLYYRNN
                     LVSLMNLLELMATYTMPNLVFSSSCTVYGQPDILPVTENSPI"
BASE COUNT          543 a          305 c          335 g          635 t
ORIGIN      
        1 aatagtcgca ttagaatctg ctgtattgtt tgaaagtggt tttgacaagt atattgataa
       61 gtctattgtg gtaacagcac cattacaggt acgcattaat agaattatga aaagagataa
      121 acttacttat ccagaggtag aagcaagaat taaaaatcaa ctttctgatg aagaaagaga
      181 agcaagagcc gattttgtta tcaaaaacga tggggtggag cacttgcctt ctcaattatt
      241 tgcttttttg aaggcagttg atttttaaac tttctgcact ttgtaatggc aattaaattt
      301 acctttcgtt aatgcgttca gttttgacga aatcatttgc ctgcgtatag gtgatatctt
      361 atctacaaat acatgccctt cgatatggtc gtattcgtgt tgcattactc tgctagggaa
      421 accactgtat tgctcaacgt gtttttgcca attttcgtca tagtattcaa ttgaaatttt
      481 gtcgcttctt tttactgttt cagaaatacc aggtatgctt aagcatcctt cttcttgaga
      541 taattcttct ccttcaaaag atgtaattaa aggattaatc attactttct tgaaatttac
      601 aaaacttggt tcgtcatcac ctaaaattga caaatcaaca ataaataaac gaatagggag
      661 tcccacttgt ggtgcggcta atcctactcc ctctgcatga gaaagggtat caaacatgtc
      721 gttgattaat gaactgagtt ctgggtagtt tttgtcaatt gggtcagctt ttttgcgtaa
      781 tacagcattg ccataaacaa ctataggacg tatcataatg tgttagtttt tatactttca
      841 aggtatgttt gtaaaatgat ggttgcacta atggtatcca ccaaagcttt gttttgtcta
      901 tctttctttt ttaatccagc ctcgagcatg gtacgatgag ctaacacaga tgtaaatcgc
      961 tcatctacgt attcaatggg aatatgcgtg attctctttt tgagcgtatg tacaaacggt
     1021 gtgatgtatt tcatcgattc agatagctca ttattcattt gtttgggtaa tcctataatg
     1081 attttatcca cgggttctgt ttgtatatag tgttctaaaa aagcaataac ttcgtgactt
     1141 gccaccgttg ttaatccgtt ggcaatcagt tgtaaagtgt cggtaacagc tatccctaca
     1201 cgttttttgc cataatcaat agcaataatt cttcccataa ttaagcacca aaagtagcta
     1261 taaaccgcca taaaagcaaa tttactagta ttttttaaaa atttagacac ttttttaacc
     1321 cttagcatgg gcactttcta ctctgtatct gaaaaatatg tatctttgtg caaaaattta
     1381 attgtatgac aaaaattatt gttacaggag gcacgggtta tataggttcg catacagtgg
     1441 ttgagttaca acaagctggt tatgaagttg tcatcattga caacttatct aattcttctg
     1501 tagatgtgtt gcaaggtatt tttgctatca cgggcgttat gccaagtttt gaaaagattg
     1561 attgtactga ttatcaagca atggagtctg tattcaaaaa acacgcagat gcacaagcaa
     1621 ttattcattt tgctgccagt aaggcggtga atgaatcggt ggaaaagcct ttgttgtact
     1681 accgcaataa tttagtgtcg ttgatgaatc tattagaatt aatggctaca tatactatgc
     1741 caaatttggt gttttcgtca tcgtgcaccg tatatggtca accagatata ttgcctgtaa
     1801 ctgaaaattc tccaataa
//
LOCUS       DAOSMY010000317         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_65089_length_1810_cov_2.051852, whole genome shotgun sequence.
ACCESSION   DAOSMY010000317 DAOSMY010000000
VERSION     DAOSMY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65089_length_1810_cov_2.051852"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1275)
                     /locus_tag="PLD76_06590"
     CDS             complement(37..1275)
                     /locus_tag="PLD76_06590"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOU27702.1"
                     /translation="MVLINENYLKLPENFFFTEIEKRINAFNVIHPNSHVIRLGLGDV
                     CLPLPDEIISAMEVASKEMGNIDTFRGYGPETGYRFLIDKIIKHDYHTRGITLDASEV
                     FVSDGSKADLGNIGHIFGRDNIIAITDPVYPVYENAAIMGGRAGHLTEENIWSNIVYI
                     PCTAENNFTPQLPKEKVDIIYLCFPNNPTGVALDKIELKKWIDYAIENQTIIIYDGAY
                     AAYITQKDVPRSIYEIKGAKKVAIEIRSFSKTAGFTGLRCSYTIVPRELIGYSLMGEP
                     VDINKLWRRRATTYYNGTSYIIQRGAEASYSPKGKKQLQKNIDYYLKNAAIIRKELIE
                     IGFQVFGGINSPHIWIKTPKNTPSWKFFQQLLYEAQIVCIPGAGFGQFGEGYFRFSGF
                     NTRQNTIEAMERMQVWRNRI"
     gene            1369..>1810
                     /locus_tag="PLD76_06595"
     CDS             1369..>1810
                     /locus_tag="PLD76_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOU27703.1"
                     /translation="MNLAKPMQDDSYNSIAQLSEGMYKEKGSKFLSFAIPVTSVEEAK
                     DILKDYRKQYYDARHICYAYVIGYPNSVVRSSDDGEPSGTAGKPILAQIQAKQLSDVM
                     VVVVRYFGGVLLGTGGLVVAYREATVDALNNTTVYTKIVEQALTI"
BASE COUNT          528 a          331 c          342 g          609 t
ORIGIN      
        1 atattgaaaa atcgaaattg aaaacatact ttcgtgttat atcctatttc tccatacttg
       61 catacgttcc atcgcttcta tcgtattttg gcgtgtgtta aaacccgaaa aacggaaata
      121 tccttcgcca aattgcccaa accctgcacc tgggatacag actatttgtg cctcgtatag
      181 taattgctga aagaatttcc acgagggtgt atttttgggt gttttaatcc aaatatgcgg
      241 ggagttaatt cctccaaata cttgaaagcc gatttctatt aattctttgc gaataatagc
      301 tgcgttttta aggtaataat caatgttttt ctgtaattgt tttttcccct taggtgagta
      361 ggaggcttca gctcctcgtt gaatgatgta agatgttccg ttgtagtatg ttgtagcacg
      421 cctacgccac agcttgttta tatctacggg ttctcccata aggctatatc caatcaattc
      481 acgaggtact attgtgtaag agcaacgaag ccctgtaaat ccagctgttt tagaaaagct
      541 tcgtatttcg atagccactt tttttgcccc ttttatttcg tatatcgaac gaggtacatc
      601 tttttgcgtt atatacgcag cataagctcc atcgtatatt ataattgttt gattttcaat
      661 tgcatagtct atccattttt ttaattcaat tttgtctaac gctacacctg taggattgtt
      721 tggaaaacac aaatagataa tgtctacttt ttctttaggt aactgaggcg taaaattatt
      781 ttctgccgta caagggatat atacgatatt actccaaata ttttcttccg ttaaatgacc
      841 tgctcgacca cccataatgg cggcattttc atacacagga tatacagggt cggtgatggc
      901 aatgatatta tctcgtccaa aaatatgtcc aatattgccc aaatctgctt tagagccatc
      961 gcttacaaat acctcgctag catctagagt aattcctcgt gtatgatagt cgtgcttaat
     1021 tattttatca attaagaaac gatagccagt ttcaggccca tatcccctaa aagtgtcgat
     1081 gtttcccatt tccttcgagg ctacttccat cgccgatata atttcgtctg gtaggggcag
     1141 gcacacatct cccaaaccca atcgaataac atgactattt ggatgaatga cattgaaagc
     1201 attgatgcgt ttttctattt cggtgaaaaa gaaattttca ggaagtttca gataattttc
     1261 gttgattaat accatgattt gttgtttaca tcagctaatg ttatgcgaag ataaaaaaac
     1321 aaaatcaaaa acgagctatt tttaacttaa aagtgtaatt ttgtctgtat gaatttagcc
     1381 aaaccgatgc aagacgatag ttacaatagt attgctcaat tatcagaagg tatgtataaa
     1441 gagaagggta gtaaattttt gtcatttgct attccagtta cttctgtcga agaggcaaag
     1501 gatatattga aagattatag gaaacagtac tacgatgctc gccatatttg ctatgcttat
     1561 gttattggtt atccaaattc cgttgtgcgt agtagcgatg atggagaacc atctggcaca
     1621 gcaggaaaac ccattttagc tcagatacaa gccaagcaat tgtcggatgt tatggttgtt
     1681 gtggtgcgtt attttggcgg tgtattatta ggtacaggag ggttggtggt ggcttatcgt
     1741 gaagctaccg tagatgcatt gaataatact accgtttata caaaaattgt agaacaagca
     1801 ctaactattc
//
LOCUS       DAOSMY010000318         1805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_65330_length_1805_cov_2.800000, whole genome shotgun sequence.
ACCESSION   DAOSMY010000318 DAOSMY010000000
VERSION     DAOSMY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65330_length_1805_cov_2.800000"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLD76_06600"
     CDS             <1..867
                     /locus_tag="PLD76_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOU27704.1"
                     /translation="YGTENIGASVIPMSSGNTQKQLQIMQDFGATVLCCTPSYALFIA
                     DKLADMGISKDDLKVRIGVFGAEPWTENMRKELEEKLGIKAYDIYGLSEICGPGVGYE
                     CQNQSGTHLCEDQFYPEIVDPKTLQPVKQGTEGELVFTHLTKEAMPLLRYRTKDLTSL
                     NYDTCACGRTAVRMGKILGRSDDMLIIRGVNVFPSQIESVILQMPEFEPHYMIYVNRE
                     NNLDTMELHVEARPDLYSDNVGTMISLTKKLTAQLNSILGIAVKVKIVEPRSLARSEG
                     KAKRVFDNRMLK"
     gene            889..1284
                     /locus_tag="PLD76_06605"
     CDS             889..1284
                     /locus_tag="PLD76_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOU27705.1"
                     /translation="MVIQQLSIFLENKAGRLNDVAALLGKAGVNMTAFSLADNSDFGI
                     LRVIVSDPHKAQEVLKAQKFAVSLTDVIHLQIANTPGSLSNVLAVLADAGMYIEYMYA
                     FSEGNGANVIIKPDNVPNAVEALQAHKIM"
     gene            1345..>1805
                     /locus_tag="PLD76_06610"
     CDS             1345..>1805
                     /locus_tag="PLD76_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27706.1"
                     /translation="MKKILLFLALMLLLGCATKPPYVPPGGDIAGSSDYVFDITSLPE
                     ITITVPLEEWNLLLSNYDINHDNEEKVVAHFAFKKNGKVDEIANVGLRLRGNTSRKRP
                     EGYEGQPHNPINPDWRHAHFSVKFDEYVSKRTFYSIDHLILKWFKDDGMYCR"
BASE COUNT          593 a          282 c          377 g          553 t
ORIGIN      
        1 tatggtactg aaaatattgg agcatctgta atccccatgt cgagtggaaa tacccaaaaa
       61 cagttgcaaa taatgcaaga ttttggagct accgtactgt gttgtacgcc atcgtatgcc
      121 ttgtttattg ccgataagct agccgatatg ggtatttcta aagatgacct aaaggtacgc
      181 ataggcgtgt ttggtgctga accgtggaca gaaaacatgc gtaaagaatt agaagaaaaa
      241 ttgggtataa aagcctatga tatttatgga ttgagcgaaa tatgcggacc tggagttggg
      301 tacgaatgcc aaaatcaatc gggtacgcat ttatgcgaag accaattcta tcccgaaatt
      361 gtagatccca aaactttaca acctgtaaag caaggtactg agggcgaatt ggtgtttaca
      421 catttaacca aagaggctat gccattgttg cgttatcgca ccaaagattt aacttcgttg
      481 aattacgata catgtgcgtg tggacgtaca gcggtgcgta tgggtaaaat attaggcaga
      541 agcgatgata tgttgattat tagaggcgta aacgtgtttc cttctcaaat tgaatcggtt
      601 attttgcaaa tgcccgaatt tgaacctcac tacatgattt atgtaaacag agaaaataat
      661 ttggatacaa tggaattgca tgttgaagca cgtcccgatt tgtattcgga caatgtgggc
      721 acgatgattt cgttaaccaa aaaattaact gcacaattaa atagtatttt agggatagct
      781 gtaaaagtga aaattgtgga gccacgctcg ttggcacgta gcgaaggaaa agcaaaacgt
      841 gtgtttgata atcggatgtt aaaataacct ttaaaaacag ataatgatat ggtcattcaa
      901 caattgtcaa tttttttaga aaataaagca ggtagattaa atgatgtagc agccttgttg
      961 ggaaaagcag gtgttaatat gacagcattt agtttagctg ataattctga ttttggtatt
     1021 ttgcgtgtta ttgtatctga tccacacaag gctcaggagg tactcaaggc acaaaaattt
     1081 gctgtttcgc ttaccgatgt tattcattta cagatagcaa atacgcctgg ctcattatcc
     1141 aatgtacttg cagtattggc agatgctggt atgtatattg agtatatgta tgctttttcc
     1201 gaaggaaatg gagctaatgt aataatcaaa ccagataatg tgccaaatgc tgttgaagca
     1261 ttacaagcac ataaaataat gtaaaataat tagacaataa tctgtctttt tatttatttt
     1321 cgtttacaga ccttattctt atacatgaaa aaaatacttc tttttttggc tctaatgctt
     1381 ttactaggat gtgccacaaa acctccctat gtacctcctg gaggagatat tgctggaagt
     1441 agtgattatg tatttgatat tacctcattg cctgaaataa caataacggt tcctttagaa
     1501 gaatggaatt tattgctatc taattatgat attaaccatg ataatgagga aaaggtagta
     1561 gcacattttg cattcaaaaa aaatggcaaa gtagatgaaa tagccaacgt tggtttacgt
     1621 cttagaggaa ataccagtag gaaaagaccc gaagggtatg agggtcaacc acacaaccca
     1681 attaatcctg attggcgtca tgctcatttc tcagttaaat ttgacgaata tgttagtaaa
     1741 cgtacatttt atagcatcga ccatttaatt ttaaaatggt ttaaagatga tggcatgtat
     1801 tgtcg
//
LOCUS       DAOSMY010000319         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_65414_length_1803_cov_3.902746, whole genome shotgun sequence.
ACCESSION   DAOSMY010000319 DAOSMY010000000
VERSION     DAOSMY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65414_length_1803_cov_3.902746"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLD76_06615"
     CDS             complement(<1..533)
                     /locus_tag="PLD76_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOU27707.1"
                     /translation="MKQVRVRFAPSPTGPLHIGGVRTALYNYLFAKKHNGKFILRIED
                     TDTNRFVPGAEAYIIESFNWLGIQFDEGVSFGGNYGPYRQSERKEIYKVYVKELLDKQ
                     LAYIAFDTPEELDTKRAEISNFQYDASTRMQMCNALTLSANEVAQRIANGDNYVVRIK
                     IEPNQDIIVQDIIRGEV"
     gene            complement(558..>1803)
                     /gene="mutL"
                     /locus_tag="PLD76_06620"
     CDS             complement(558..>1803)
                     /gene="mutL"
                     /locus_tag="PLD76_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU27708.1"
                     /translation="VYQLASSNHRVRIGAIFGTKINQQLLPIEVNTQLISFFGFIGNP
                     SFAQKRSANQYFFVNGRYIQHPYFYKAIALAYEKLLPPDTKPSFFMYLQVDPSAIDVN
                     IHPTKTEVKFEHEQAIFPILSAAVKETLGKTNAVPSIDFDQDNALEIPVVSQDTYVAP
                     PTIHVNPSYNPFKSTNFSAPTAPKTIGWESLYKNFDAKHTPQSDNAFEHQFEEVIDNE
                     QLSLYPSHEGNKAMLQLRKHYIVLPTENGITIIHQHRAHMHLLYTQYVENMQQKRGIS
                     QQLLFPEIVELTPQEHSCLLEILDDISYIGFRLEEFGKHAYQLAGVPTQLESATCADV
                     LHALLESVQETGNTLKTDMQQTIALTLAKKAAIKTGATLSHEEMNEIVQSIHQGTIPA
                     YTTDGKKILSTISFAEIDKTFN"
BASE COUNT          488 a          309 c          384 g          622 t
ORIGIN      
        1 tttacttcgc ctctaataat gtcttgtaca ataatatctt ggtttggttc aattttgata
       61 cgaacaacat aattatcgcc attggctatg cgttgagcca cttcatttgc cgataaggta
      121 agtgcgttgc acatctgcat acgtgtgcta gcgtcgtatt gaaagttgga aatttctgct
      181 ctttttgtgt cgagttcttc gggcgtatca aaagcaatat atgccaactg tttatccaat
      241 agttctttta catagacttt ataaatttct ttgcgttcgc tttggcgata cggaccgtag
      301 ttgcctccga acgatacacc ttcgtcaaat tgaatgccga gccagttgaa agattcgata
      361 atatacgctt cagcacctgg cacaaagcgg ttggtatcgg tatcttcgat acgtaggata
      421 aattttccgt tgtgtttttt tgcaaataaa taattataca aggctgtgcg tacaccgcct
      481 atgtgcaaag gacctgtggg agagggagca aaacgaactc taacttgttt catgaatctt
      541 gtatctaaat gtgttgttta attaaacgtt ttatctattt cggcaaatga aattgttgat
      601 agtatttttt taccatcagt tgtatatgcg ggtattgttc cttgatgaat ggattgtact
      661 atttcattca tttcttcgtg cgacaatgtt gcacctgttt taatagcagc ttttttcgct
      721 aaggtaaggg ctattgtttg ttgcatatct gttttaagag tatttcctgt ctcttgaaca
      781 gattcgagta aggcatgcaa cacatctgca caagtagcac tctctaattg agtaggaacc
      841 cctgcaagtt ggtaggcatg tttgccaaac tcctcaagac ggaacccaat atacgatatg
      901 tcatcaagta tttcgagcaa gcaactatgc tcttgaggtg tgagttctac tatttccgga
      961 aacaatagtt gttgcgaaat acctcgtttt tgttgcatat tttccacata ctgagtatat
     1021 aataagtgca tgtgagctct atgctggtga atgatggtaa tgccattttc ggttggcaat
     1081 acaatatagt gtttgcgaag ttgcaacata gctttgttgc cttcgtgcga agggtataat
     1141 gaaagttgtt cattgtcaat tacttcctca aattgatgct caaacgcatt atcagactga
     1201 ggagtatgtt tagcatcaaa atttttgtac aacgattccc agccaattgt tttaggagct
     1261 gtgggtgctg aaaaattcgt tgatttaaat gggttgtaag atgggttaac gtgtatcgtt
     1321 ggtggagcca catatgtatc ttggcttaca acgggtattt cgagtgcatt atcttggtca
     1381 aaatcaatag agggcactgc atttgttttg cctaaggttt cttttaccgc tgctgataga
     1441 attggaaaaa tggcttgttc gtgctcaaat ttcacttctg ttttcgttgg atgaatgttt
     1501 acatcaattg ccgatgggtc aacttgcaaa tacatgaaaa aagaaggttt ggtatctggt
     1561 ggtagtagtt tttcgtatgc caatgctatg gctttgtaaa aatagggatg ttgaatgtac
     1621 ctaccattta caaaaaaata ctgatttgcc gatcgttttt gagcaaaaga aggattacct
     1681 ataaacccaa aaaatgagat aagctgtgtg tttacttcta tcggaagcaa ttgttggttg
     1741 attttagtgc caaaaatagc tccaatacgt acgcggtggt tgctactagc tagttgatat
     1801 act
//
LOCUS       DAOSMY010000320         1792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_66005_length_1792_cov_2.419689, whole genome shotgun sequence.
ACCESSION   DAOSMY010000320 DAOSMY010000000
VERSION     DAOSMY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66005_length_1792_cov_2.419689"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="obgE"
                     /locus_tag="PLD76_06625"
     CDS             <1..1090
                     /gene="obgE"
                     /locus_tag="PLD76_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU27709.1"
                     /translation="NFVDYVKIYCRSGKGGAGSMHLHREKFITKGGPDGGDGGRGGHI
                     ILRANANYWTLLHLKYARHVFAGHGEKGSGSRSFGKDGEDQIIEVPCGTVAYDAETGE
                     FVCDVTDHGQEVILLKGGRGGLGNWHFRSATNQTPRYAQPGEPMQEKTIILELKILAD
                     VGLVGFPNAGKSTLLSVVSAAKPKIDNYAFTTLEPNLGIVSYHDHKSFVMADIPGIIE
                     GAHEGRGLGLRFLRHIERNSVLLFMVPADSADIHKEYRVLLNEIEQYNPELLDKRRVL
                     AISKSDMIDDELELAMKKDLPSIPCVFISSITGKGIAELKDKLWDEIMAEPIKDIVHK
                     SKFVEIADDEVVDDEEPFYEEDSEEDDE"
     gene            1179..1493
                     /locus_tag="PLD76_06630"
     CDS             1179..1493
                     /locus_tag="PLD76_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU27710.1"
                     /translation="MNIFVGSLSFNVKDDQLKAAFENFGEVSSARVLTDKFTGRSKGF
                     GFVDMPDNAAAQKAIDALNGTELEGRAMVVNEARERTEDSRRPSTGGYQKRDNSGFKK
                     RY"
BASE COUNT          598 a          258 c          398 g          538 t
ORIGIN      
        1 aaattttgta gattacgtaa aaatctattg tcgttcgggt aaaggtggtg caggttcgat
       61 gcacttacac agggaaaaat ttattaccaa aggaggacct gatggaggtg atggaggtag
      121 agggggacat attatattaa gagcgaatgc caattattgg actttgttgc acttaaaata
      181 tgctcgtcac gtatttgctg gacacggcga aaaaggcagt ggtagtcgaa gttttggtaa
      241 agatggtgaa gaccaaataa ttgaagttcc ttgtggcact gtagcttatg atgccgaaac
      301 gggagaattt gtatgtgatg taactgacca tggtcaagaa gtcattttgc tcaaaggagg
      361 aagaggaggt ttaggtaatt ggcattttcg ctcggcaacg aatcaaactc ctcgttacgc
      421 tcaaccagga gagccaatgc aggaaaaaac cattattttg gaacttaaaa ttcttgctga
      481 tgtagggcta gttggtttcc cgaatgcagg aaaatctacc ttactttcag tagtatccgc
      541 agccaaaccc aaaattgata attatgcttt tacaacttta gaaccgaatt taggcattgt
      601 aagttatcat gaccataaat cgtttgtcat ggcagatatt cctggtatta ttgaaggagc
      661 acacgagggt agaggtttag gtttacgatt tttacgccat atcgaacgta attccgtgtt
      721 attatttatg gttccagctg atagtgcgga tattcataaa gaataccgtg tattacttaa
      781 tgaaatagaa caatataatc ctgaactatt agacaaacgt agggtattgg caattagtaa
      841 gtcagatatg attgatgatg aattagagtt ggcgatgaaa aaggacttac caagcattcc
      901 ttgtgtattt atatcttcaa ttactggaaa aggtattgct gaattgaaag ataaattgtg
      961 ggatgaaatt atggctgaac caattaaaga cattgtacac aaatctaaat ttgttgaaat
     1021 agctgatgat gaggtggttg atgatgaaga acctttctac gaagaagata gcgaggaaga
     1081 tgatgaataa gcttaaaaca atcttatttt ttagataata gtttgtaaac aagaaaaata
     1141 cattatcttt gtcgaattat ttttaataca tataacgaat gaacattttt gtaggaagtc
     1201 tatcttttaa cgtaaaagat gaccaattga aagcagcctt tgagaatttt ggagaagtat
     1261 cttcagcacg agttttaact gataaattta ctggaagaag taaaggtttt ggatttgttg
     1321 atatgccgga taatgcagca gcacaaaaag ctatcgacgc attaaatggt acagaactag
     1381 aaggtcgtgc aatggtagta aacgaggcta gagaaagaac tgaggactct cgcagaccaa
     1441 gtactggtgg ttaccaaaag agagataaca gcggttttaa aaagagatat taataaggtg
     1501 taaatcttta ttatgagaaa aagagcgttt tattatttaa aatgctcttt ttgtttgtaa
     1561 ttagctttgt tcaaaggata atttacaaag aaaaagattg tatttttgtg ttttgatgta
     1621 atacaacttg aaacatgcca ggtacactag ttaatttatc ctctttttta cagcaacaac
     1681 tagcggtggc gcaacaacgg tataaaaaac catcccagcg tcaacatcct gcattcggtt
     1741 cgtggcgggg acggcaaatt gaggcgcttg attatcagca catgctccgt tc
//
LOCUS       DAOSMY010000321         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_66621_length_1780_cov_3.382029, whole genome shotgun sequence.
ACCESSION   DAOSMY010000321 DAOSMY010000000
VERSION     DAOSMY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66621_length_1780_cov_3.382029"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1429
                     /gene="mtaB"
                     /locus_tag="PLD76_06635"
     CDS             131..1429
                     /gene="mtaB"
                     /locus_tag="PLD76_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU27711.1"
                     /translation="MIDTSVFDNKTVAFYTLGCKLNFSETSAIGKSLIEQGYRKVAPH
                     EPASLCIINTCSVTDSADKKCRQTITKAIKENPGALVIVTGCYAQLKPDDITSIPNVD
                     LVLGANDKFNIPDIIAKKTAMPNKLQASPTNRMTEFHHSYSSEDRTRHFLKVQDGCDY
                     FCSYCTIPLARGRSRNGSIENLVKDAQSIAALGGKEIVLTGVNIGDFGHSTNESFIDL
                     LKALEQVEGIERYRISSIEPNLLTDDIIALVAQSTKLMPHFHIPLQSGCNEVLNLMKR
                     RYTTELFAQKIATIKQLMPHAFIGVDVIVGVRGETTSYFETTYQFIESLPISQLHVFS
                     YSERAGTQALKIEHVVTPNEKKRRSHLLLSLSEQKTEAFYTNFKHTIRPVLWEGTKKG
                     GFMYGFTDNYIRVKTPYVIHKINTIESIILTAETISIEKS"
     gene            1434..>1780
                     /locus_tag="PLD76_06640"
     CDS             1434..>1780
                     /locus_tag="PLD76_06640"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU27712.1"
                     /translation="MTFELQHTDPKSNARAGLITTDHGQIETPIFMPVGTVASVKAVH
                     QHELKEDIKAQIILGNTYHLYLRPGIEILEKAGGLHKFNGWDKPILTDSGGFQVFSLS
                     HRRKMKEEGVTFHS"
BASE COUNT          641 a          324 c          320 g          495 t
ORIGIN      
        1 atatatccac ctaaaaaaca tagctataaa acgttaagga atattcattt ataacaacaa
       61 atagatgcaa tacagcaaaa aaatagtact tttgtatcct gattttgaat gattccatgt
      121 acatataatt atgattgaca catctgtttt tgacaataaa acggtagctt tttatacact
      181 aggttgcaaa ctgaattttt cggaaacatc tgctataggt aaaagtttga ttgagcaagg
      241 gtatcgcaaa gtagcccctc acgagccagc ctcgctttgt attattaaca cctgctcggt
      301 aacagatagt gctgataaga aatgtagaca aactattacc aaagccatta aagaaaaccc
      361 aggagcttta gttattgtta caggttgtta tgctcaactc aaacccgatg atattacttc
      421 tatacccaat gttgatttgg tgttgggagc aaacgataaa ttcaatattc ctgatattat
      481 agcaaagaag actgctatgc ccaacaaatt gcaagctagc cctaccaacc gaatgactga
      541 atttcatcat tcatattcga gcgaagacag gacaaggcat tttttaaaag tacaagatgg
      601 gtgtgattat ttttgtagct actgtactat tccattggct agaggcagaa gcagaaatgg
      661 ctctatcgaa aatttagtaa aagatgcaca aagcattgca gcacttggtg gtaaagaaat
      721 agtattaacg ggcgtcaata ttggtgattt tggacattca acaaacgaat catttattga
      781 tttattaaag gcacttgaac aagtagaagg aatagaacgt tataggatat cttctattga
      841 accaaattta ttaacagatg atattattgc tctagtggca caatctacca aactaatgcc
      901 tcattttcat attccactac agtcgggctg taatgaggta ttgaacttaa tgaaaagacg
      961 ctacacaacc gaattgtttg ctcaaaaaat tgccactata aaacaactga tgccacacgc
     1021 atttattggt gtagatgtaa ttgtaggcgt acgaggagaa accacatcct attttgaaac
     1081 aacttaccaa ttcattgaat ctttacctat ttcacaattg catgtatttt cgtattcgga
     1141 aagagcaggc acacaagcat taaaaattga gcatgtggta acacccaacg aaaaaaaaag
     1201 acggagccac ttattactct cgttatccga gcaaaagaca gaggcttttt acactaactt
     1261 taagcatacc atccgacccg ttttatggga aggcactaaa aaaggcggtt ttatgtatgg
     1321 atttacagat aactatattc gtgtaaaaac gccttacgta atacataaaa ttaatacgat
     1381 tgagtctatt atattaaccg cagaaacgat tagcatagaa aaaagttaat gaaatgacat
     1441 ttgaactaca acataccgac cccaaaagta atgctcgagc aggcttaata acaaccgacc
     1501 atggacaaat agaaacaccc atttttatgc ccgtaggtac agtagcatca gtaaaagcag
     1561 tacatcaaca cgagttgaaa gaggatataa aagcacaaat catcttagga aacacctacc
     1621 atttatacct ccgaccaggt atcgagattc tggaaaaagc aggaggctta cataaattta
     1681 acggatggga caagcctata cttacggata gtggtggttt tcaggtattt tcactgtcgc
     1741 atagacgaaa aatgaaagaa gagggcgtaa cctttcattc
//
LOCUS       DAOSMY010000322         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_66723_length_1778_cov_3.175276, whole genome shotgun sequence.
ACCESSION   DAOSMY010000322 DAOSMY010000000
VERSION     DAOSMY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66723_length_1778_cov_3.175276"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLD76_06645"
     CDS             complement(<1..474)
                     /locus_tag="PLD76_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27713.1"
                     /translation="MKKILLIAFSLLSISAFAKNCENNPHRIDKVHIPAQLSQYNLFG
                     STNDVIDVAFIVSPTKIKVTLKNETPYNQGDISLYTYNYAKKPTTESFRLGTKITPAF
                     TQKGNIITIDLASLNLNTNYLIDIKGNQSPVFLDHAIGGILDTYFDASNEQNLGVN"
     gene            866..1048
                     /locus_tag="PLD76_06650"
                     /pseudo
     CDS             866..1048
                     /locus_tag="PLD76_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(1094..1465)
                     /locus_tag="PLD76_06655"
     CDS             complement(1094..1465)
                     /locus_tag="PLD76_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU27714.1"
                     /translation="MIIQFIKFCVVGASGLLVDFGITYLCKEKIKLNKYIANSLGFIA
                     AASSNYLLNRMWTFENTDPNISTQYLLFIIISLIGLGINNGVIYLVLRKRDYNFYIVK
                     VFAIAVVTLWNFFMNFFFTFQ"
     gene            complement(1566..>1778)
                     /locus_tag="PLD76_06660"
     CDS             complement(1566..>1778)
                     /locus_tag="PLD76_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27715.1"
                     /translation="KPWSWVEYFPVDVENPLILTAFSTTNIQQKISIESEKAKPNETY
                     ETGFLLTKPPYIVWYNSPIYNIHIVP"
BASE COUNT          553 a          255 c          339 g          631 t
ORIGIN      
        1 gttaacacct aaattttgtt cgttagatgc atcaaaatag gtatctaaaa taccaccaat
       61 agcatggtcg aggaatacgg gcgattgatt tcctttgata tcaattaggt aattggtgtt
      121 taagttgagc gatgctaggt cgatggtaat gatgttgcct ttttgtgtaa aagcaggcgt
      181 aattttagtg cctaaacgaa agctttctgt ggtcggtttt ttagcgtagt tatacgtgta
      241 aagagatata tctccttggt tgtaaggtgt ttcgtttttt agggttactt ttattttggt
      301 aggcgatacg ataaaggcta cgtctattac atcgttggta gagccaaaca aattatactg
      361 acttaattgt gctggtatat gcaccttgtc gatgcgatgt ggattatttt cgcagttttt
      421 tgcaaaagca cttatactca gcaaagagaa agcaataagt aggatttttt tcatatgatt
      481 acactattga ttggttaatt attatctgct atttttttat cacggtaaaa atagcaaagt
      541 tttttttatt tgcaaaaaaa atacgaattt acacaccttt tattatgcag atagtcatgg
      601 ttttaatgcg aatgtaaaat agtattgata gtttgttggt atcataccta aaaccttgta
      661 tatttgcaca atctaaaata attggatgtt tacgttaaat tcggatttgg ttaagtaaat
      721 aagtgtaggc aattttttat acttatccct acaactggta ctcaggcata ttcttgtccg
      781 agtagtttat cataactatt tattagttgg cattgaatac gtatgtattt tagccatatt
      841 tattaacgaa acaattttaa ttatcatgag taacgaaaat aaatcaattc acgaatttga
      901 ttatcaatta atttgcgact atttttcacg tatcgaacgt caaggaccag gtagtcctga
      961 agtaaccgta aaagccttga gttttatcga caagctaaat gaaacctcaa aaattgcaga
     1021 tttaggttgt ggtacaggtg ggcaatccgc ttgttttaac ggatagtgag tgaatcaaat
     1081 tcgttgcatc ttttcactga aaagtaaaaa agaagttcat aaagaagttc caaagtgtta
     1141 ccactgcgat ggcaaaaact ttaactatgt aaaaattgta gtctcgtttg cgaagcacca
     1201 aatatatcac tccattgtta atgcccaaac caataagaga aataatgata aacagcagat
     1261 attgtgtaga gatgttgggg tcagtgttct caaatgtcca catgcgattg agtaggtagt
     1321 tgctactagc tgcagcaata aagcctaatg aattggcaat gtatttattg agcttaattt
     1381 tttctttaca cagataggta atgccaaaat cgaccaataa gccagatgct cctactacac
     1441 aaaatttgat aaattgaata atcattgttc tgttatacta tttataatgg caaagataaa
     1501 taaaaggaat tcattgtagg tgcttttttg tgcttttttc tttttagctt actatataca
     1561 gtttttcagg gtactatatg tatgttatat atggggctgt tgtaccacac aatatatggt
     1621 ggtttggtaa gcaaaaagcc tgtttcgtat gtttcatttg gcttggcttt ttcgctttca
     1681 atactgattt tttgttggat gttggtggtt gaaaatgctg ttaaaataag cgggttctct
     1741 acatcaacag gaaagtattc aacccacgac caaggttt
//
LOCUS       DAOSMY010000323         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_66991_length_1773_cov_3.279977, whole genome shotgun sequence.
ACCESSION   DAOSMY010000323 DAOSMY010000000
VERSION     DAOSMY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66991_length_1773_cov_3.279977"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..850)
                     /locus_tag="PLD76_06665"
     CDS             complement(176..850)
                     /locus_tag="PLD76_06665"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOU27716.1"
                     /translation="MKNIFIYVCSCFLCIVSVLNTNAQELSNPEKLSMPLYQGSFIGL
                     NLAPSVESFFVPSYFSTEVSIDVSLKQKYFPIVEAGIENRKLLTSDYTYNSSGYFAKF
                     GINYNFLKTSTLVPNNIFYIGARYAIASTTYELATQVTESYWNEQGEVSLMPTKSLAT
                     WGEIVLGVRAELFPSVLLGTSVRYGFAPSLSNSLLGYPTFIPNYGLFLPNKWLFTYSI
                     TYKLPF"
     gene            complement(822..1301)
                     /locus_tag="PLD76_06670"
     CDS             complement(822..1301)
                     /locus_tag="PLD76_06670"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOU27717.1"
                     /translation="MEITSLVKNIYNRLIVFICLACIFSACVEKECITYKHVLVGIEL
                     IQDTSNTVYTADSITVKGVGSDEIIYDNAKNISSIYLPLKSVSNTTSFDIVLNETPFV
                     LSITHQNIPQLLSIECGTVMFHKIEDATIDGEIQAVIQVENTHVQNRENEKHIYIRL"
     gene            complement(1292..>1773)
                     /locus_tag="PLD76_06675"
     CDS             complement(1292..>1773)
                     /locus_tag="PLD76_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU27718.1"
                     /translation="RYSVRSFLPTQVEKEKIDYLLECARWAPSAVNAQPWHIIVVQQE
                     KNKRALHQCYQREWFKQAPMYMLVCGNHQESWKRKYDNNDHCSIDIAIAVEHIALAVQ
                     EVGLGTCWVCNFDAVLCAELFELPNHIEPIVILPIGYPNDSLNEKKRKSIQDIVEWK"
BASE COUNT          607 a          322 c          262 g          582 t
ORIGIN      
        1 ctcctggatg accagatttg gctttttcta ccatagatgc tgataaaata cgtatgttat
       61 cggctgcttt atttactaga tgtatatcag acatatcatt tatatattaa tttcgattca
      121 aaaaaattag aaagtgcaaa gttacaagaa taatcacttt ctacaaaaaa aaatatcaaa
      181 atggtaattt atatgttata gaatatgtaa ataaccactt attgggtaaa aataatccat
      241 aatttggtat aaaagttgga tatcccagca aagaatttga gagcgaagga gcaaatccgt
      301 aacgaacact tgtacctaat aatactgatg gaaacaattc tgctctcact cctaatacta
      361 tttctcccca agttgcaagg ctcttcgttg gcataagaga aacctctcct tgttcattcc
      421 aataactttc agtaacctga gttgccaatt cgtaagtagt agaggctata gcatagcgtg
      481 ctcctatgta aaatatatta ttgggaacaa gtgtagaagt tttcaaaaaa ttataattaa
      541 tcccaaactt agcaaaatat cctgatgaat tataggtata atcggaagtt aatagttttc
      601 tattttcaat acctgcctca actataggaa aatatttttg ttttaaacta acatctatcg
      661 aaacttctgt tgaaaaatag gatggtacaa agaaactctc tacagacggt gcaagattta
      721 atccaataaa agagccttga tacaacggca tggaaagttt ctctggatta gaaagttcct
      781 gtgcattcgt attgagtaca gaaactatgc acagaaaaca actacaaacg tatataaata
      841 tgtttttcat tctctcgatt ttgtacatgt gtattctcaa cttgaataac tgcttgtatt
      901 tctccatcaa tagtagcatc ctctatttta tgaaacatta ctgttccaca ttcgatgctc
      961 aaaagctgag ggatattctg atgtgtaata gataaaacaa agggagtttc attcaataca
     1021 atatcaaaac tagtggtgtt actaactgat tttaatggta aataaatgga tgaaatgttt
     1081 tttgcattat cgtaaataat ttcatccgat cctactcctt ttaccgtaat actatccgca
     1141 gtatacacag tattggatgt atcttgtatc aattcaatgc ccactaaaac atgtttataa
     1201 gtaatgcatt ccttttctac acatgcagaa aaaatacatg ccaaacaaat aaatacaatc
     1261 aatctattat atatgttttt caccaaacta gttatttcca ttcaacaata tcttgtatcg
     1321 attttcgttt cttttcattc agagaatcgt taggataccc tatcggcaaa atgacaatag
     1381 gctctatgtg attaggtaat tcaaataact cggcacacaa tacagcatca aaattacaca
     1441 cccaacatgt ccccaaacca acttcctgaa ctgctaaagc aatatgttca actgcaatag
     1501 caatatctat ggaacaatgg tcgttattat catattttcg tttccacgat tcttgatgat
     1561 taccacacac aagcatatac ataggagctt gtttgaacca ttctctttga taacattggt
     1621 ggagggctcg tttattcttt tcttgttgca caactattat atgccatggt tgagcgttaa
     1681 ccgccgaagg agcccatcta gcacattcta acaaataatc aattttttct ttttctacct
     1741 gtgttggcaa gaaagacctt acagaatatc ttg
//
LOCUS       DAOSMY010000324         1750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_68233_length_1750_cov_2.956342, whole genome shotgun sequence.
ACCESSION   DAOSMY010000324 DAOSMY010000000
VERSION     DAOSMY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68233_length_1750_cov_2.956342"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLD76_06680"
     CDS             <1..316
                     /locus_tag="PLD76_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU27719.1"
                     /translation="LSPEIKEVVTATLEIMEVFKITKVGNVAGCLVREGKIKRTNKIR
                     LIRDGIVIYSGQLGSLKRMKEDVKEVALGYECGLNIGNYNDLRVGDIIESFEEVEVKK
                     TL"
     gene            407..907
                     /locus_tag="PLD76_06685"
     CDS             407..907
                     /locus_tag="PLD76_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU27720.1"
                     /translation="MENYIDIVLIIPLIWGLIRGLFKGFIMTVGSIIGFILGFYVSNN
                     YALSLSSYLQSWFSLSTQIAYVFAYLMIFLVVILSIFSISLLLEKTIKHISLGWLNKV
                     IGAIVGCLKYALIVSLLLNLVEIIDEKVDIVAEETKQSSYMYKPLLQIVPAILPSITY
                     YEDKKL"
     gene            910..>1750
                     /gene="sufB"
                     /locus_tag="PLD76_06690"
     CDS             910..>1750
                     /gene="sufB"
                     /locus_tag="PLD76_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOU27721.1"
                     /translation="MDEKNIIEDVTSSEYKYGFTTEIETDVIPIGLNEDVVRIISAKK
                     NEPDWMLDFRLKAYRYWITQKMPSWANLSLPEIDYQAISYYAAPRSTMPQSIDDVDPE
                     VIKTFDKLGIPLHERDKLLGIAVDAVMDSVSVKTTYKESLAELGIIFCSFSEAVTNYP
                     DLVKKYLGSVVPYTDNFFAALNCAVFSDGSFVYIPKGVRCPMELSTYFRINAANTGQF
                     ERTLIVAEEDSYVSYLEGCTAPMRDENQLHAAIVEIVILDRAEVKYSTVQNWYPGDKN
                     GVGG"
BASE COUNT          574 a          232 c          346 g          598 t
ORIGIN      
        1 gctgtcgcct gagatcaaag aagttgtaac tgctacgttg gaaataatgg aggtgtttaa
       61 aattacgaaa gtagggaatg tggcagggtg tttagttcgt gaaggaaaaa tcaaaagaac
      121 aaacaaaatc cgcctaattc gtgatggtat agtgatatat tcaggtcaat taggttcttt
      181 gaaacggatg aaggaagatg taaaagaagt ggcattagga tatgagtgtg gattgaatat
      241 tggtaattac aatgatttac gtgtaggaga tattatagaa tcgtttgaag aagtagaagt
      301 taaaaaaaca ttgtaatatt ttttattatt attatctgca ttcacattaa aactgatttt
      361 tttttggatt aatgtaaatg cagatttttt tttaatatta tttaagatgg aaaattacat
      421 agatattgta ttgattattc cgttgatttg ggggttaatt cgaggtttat ttaagggttt
      481 tataatgacc gttggctcta taattggttt tatactaggt ttttatgtat caaacaacta
      541 tgctttgtct ttaagctcat atttgcaatc ttggttttct ctgtctactc agattgcata
      601 tgtttttgca tatcttatga tttttttagt agttattctc tcaatttttt ccatatcttt
      661 attgctcgaa aaaacaatca aacatatttc gcttggttgg ttaaataaag taataggagc
      721 gatagtaggg tgcttgaaat atgcacttat agtaagcctt ttacttaatt tagttgaaat
      781 tatagatgaa aaggtcgata ttgttgcaga agaaactaag cagtcttcat atatgtacaa
      841 acctttatta cagattgtac ctgcaatatt gccttcaatt acctattacg aagataaaaa
      901 attataacaa tggacgaaaa aaatataatt gaagacgtaa catctagtga atataaatat
      961 ggattcacta cagaaattga aacagatgtt attcctattg gcttaaacga agatgtagta
     1021 cgaattattt ctgccaaaaa gaatgaaccc gactggatgt tggatttcag actaaaagcg
     1081 tatcgttatt ggataacaca aaaaatgcca agctgggcaa atttatcctt gccagaaatt
     1141 gattatcaag caatttccta ttatgctgca ccacgttcta ccatgcccca aagtattgat
     1201 gatgtagatc ccgaagtaat aaaaacgttt gataaactcg gcattccgtt acatgagagg
     1261 gataagctac ttggaattgc tgtggatgca gtaatggata gtgtatctgt aaaaactacc
     1321 tataaggaga gtttagctga gttaggtatt attttttgct cttttagcga agcagttacc
     1381 aattatccag atttggtgaa aaaataccta ggctctgtag taccttatac agataatttc
     1441 tttgctgctc ttaattgtgc cgtttttagt gatggctctt ttgtgtatat accaaaagga
     1501 gtgcgttgtc ccatggagtt atcaacctat tttagaatta atgcagctaa tacaggtcaa
     1561 tttgaacgta cccttattgt tgcagaagaa gattcgtacg tttcttatct agagggatgt
     1621 acagctccaa tgagagatga aaatcaactg catgcagcca tcgtagagat tgtgatatta
     1681 gatagggctg aagtaaaata ttcaacagtt caaaattggt atccaggtga taaaaatggc
     1741 gttggtggta
//
LOCUS       DAOSMY010000325         1748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_68337_length_1748_cov_3.510337, whole genome shotgun sequence.
ACCESSION   DAOSMY010000325 DAOSMY010000000
VERSION     DAOSMY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68337_length_1748_cov_3.510337"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLD76_06695"
     CDS             <1..703
                     /locus_tag="PLD76_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU27722.1"
                     /translation="RIVEIAYLKIYPNGNEESKRYLVNPEMPIPQEVTAIHGISDEDV
                     KNEPVFKELAKIIAKDIEGCDLAGYNSARFDLPLLAEEFLRANVDIDLAKRKMIDVQV
                     IFHKMEQRTLSAAYKFYCNSELTNAHTAEADTRATYEVLKAQLDRYDNLKNDVAFLAD
                     FSAQHKNADLAGRIIFNDKNEEVFNFGKYKGRRVEDVLNEDMGYYGWIMNGDFSLYTK
                     KVLTEVKLRGFTTKR"
     gene            complement(777..1724)
                     /locus_tag="PLD76_06700"
     CDS             complement(777..1724)
                     /locus_tag="PLD76_06700"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU27723.1"
                     /translation="MNALTKTDFNFDKQKSVYHGKVRDVYNINDELLVMVVSDRISAF
                     DVVLPKGIPYKGQMLNQIAAKFLDATADIVPNWKLATPDPMVTVGVHCDSFPVEMIVR
                     GYLCGSAWRAYQKGVREICGVKIPDGMRENQRFAEPIITPTTKADQGFHDEDISKEEI
                     LKQGLVSAEDYALLEKYTMQLFLRGTEMALERGLILVDTKYEFGKKNGQIYLIDEIHT
                     PDSSRYFYASEYQARFEKGEQQKQLSKEFVREWLMENGFQGKEGQQVPEMTDEIVASI
                     SERYIELFENISGEKFIKAPTENILERIEKNVSKYLATR"
BASE COUNT          529 a          330 c          332 g          557 t
ORIGIN      
        1 tagaatcgtt gaaatcgcgt atttgaaaat ttatccaaat ggcaacgaag aatcaaaacg
       61 atatttggtt aatccagaaa tgcctatccc ccaagaggtt accgctattc atggtatcag
      121 tgatgaggat gtaaaaaatg aacccgtttt taaagaactt gccaaaataa ttgccaaaga
      181 tattgaaggg tgcgatttgg ctggatacaa ttcggcgcgt tttgatttgc ccttattggc
      241 cgaagaattt ttgagagcaa acgtagatat tgatttggcg aaacgcaaaa tgatagatgt
      301 gcaagtgatt tttcacaaga tggaacaacg taccttgtcg gcagcataca aattttattg
      361 caatagcgaa ttaaccaatg cacacacagc cgaagctgat acacgtgcta cctacgaagt
      421 attaaaagct caattagata ggtatgataa tttgaaaaac gatgtggctt ttttagctga
      481 cttttcggct caacacaaaa atgctgattt ggcagggaga attattttca acgacaaaaa
      541 tgaggaagtt tttaattttg gtaaatacaa agggcgtagg gtagaagatg tgttgaacga
      601 agatatgggc tactatggct ggataatgaa tggcgatttt tcgctctata ccaaaaaagt
      661 gcttaccgaa gtaaaactaa gaggatttac cactaaacgc tagcttagaa tacacaaaaa
      721 aacagaggat atgtttcaag tatatcctct gtttttttat tattttcagt aagtaattat
      781 ctagtagcta aatacttgct tacattcttc tcaatccgtt caaggatatt ctctgtggga
      841 gctttgataa atttttcgcc agatatattt tcaaataact cgatataacg ctcgctgatg
      901 ctggctacaa tctcatctgt catttcaggt acttgttgtc cttctttgcc ttgaaaaccg
      961 ttttccatca gccattcgcg tacaaactct ttcgataatt gtttttgttg ttctcctttt
     1021 tcaaaacgtg cttggtattc ggaggcataa aaatagcgag aagaatcggg cgtatgaatt
     1081 tcgtcaatca aataaatttg accatttttt ttgccaaact catattttgt atctaccaaa
     1141 attaatccac gctctaaagc catttcagta cctcttagaa atagttgcat ggtgtatttt
     1201 tcgagcaaag catagtcctc agccgacaca agtccttgtt ttagaatctc ttctttcgat
     1261 atatcttcgt cgtgaaaacc ttgatctgct tttgtggtag gagtaataat tggctcagca
     1321 aaacgttgat tttcacgcat gccatcaggt atttttacac cacaaatctc acgtacacct
     1381 ttttggtagg ctctccatgc actgccacac aagtagccac gcacaatcat ttctacagga
     1441 aacgaatcgc aatgcacacc cactgttacc attgggtcgg gtgtagccaa tttccagttt
     1501 ggcacaatat cagcagtagc atccaaaaat ttggcagcaa tttggttcag catttgtcct
     1561 ttgtaaggaa tacctttggg caatactaca tcaaaagccg aaatacggtc gcttactacc
     1621 attaccaata attcgtcgtt aatgttatac acatcacgta ctttgccatg atacacgctt
     1681 ttttgcttgt cgaagttgaa atcagttttt gttagtgcat tcattgttgt tagggtttag
     1741 agtgggtt
//
LOCUS       DAOSMY010000326         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_68763_length_1741_cov_2.743179, whole genome shotgun sequence.
ACCESSION   DAOSMY010000326 DAOSMY010000000
VERSION     DAOSMY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68763_length_1741_cov_2.743179"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLD76_06705"
     CDS             complement(<1..358)
                     /locus_tag="PLD76_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU27724.1"
                     /translation="MKNKPISPWAWIPSLYFGQGIPYVVAATTFSLVMYKNMGISNAD
                     AALYTSWLYLPWVLKPFWSPFVDLIKTKRWWILLMQFVIGIALAGVAFAIPLPYFLQS
                     TLAILWLIAFSSATHDI"
     gene            complement(542..>1741)
                     /locus_tag="PLD76_06710"
     CDS             complement(542..>1741)
                     /locus_tag="PLD76_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOU27725.1"
                     /translation="EQVQGECIVSCVNGKILFNNQLYNELCFEPNDANVGTFLVYDVV
                     IGVDFHWQRKENQTFKGTLRFVVEQDNIVLINTLPVEEYLLSVISSEMSATSSISLLK
                     AHAVISRSWLFAQIQQVCSSQAETLSIEGNTMIKWYDHHNHLLFDVCADDHCQRYQGV
                     AKVTTNQVQKAIEETYGEVLVYQNNLCDARFSKCCGGVTEEYATCWENNQVAYLQSIV
                     DEKQEEKKLDLRTESAITSWIKSSPTVFCNTSDAHILSQILPHFDQETTDFFRWKKVY
                     SQHELSTLVHKRSGIDFGVIHDIRPIKRGKSGRLEIIEIVGTTRTMRIGKELEIRKWL
                     SDTHLYSSAFIVERDDERNFIFYGAGWGHGVGLCQIGAAVMSEQGYSYKEILQHYYSG
                     VDILSVY"
BASE COUNT          559 a          368 c          268 g          546 t
ORIGIN      
        1 ctatatcgtg cgtagctgaa ctaaaggcta ttaaccataa tatagccaat gtactttgca
       61 aaaaataggg caagggaatg gcgaaggcaa cgccagccaa tgctattcct atcacaaact
      121 gcataagcaa tatccaccag cgttttgttt tgattaaatc gacaaacggg ctccaaaagg
      181 gtttaagtac ccaaggcaaa tacaaccagc tagtatataa ggctgcatcg gcatttgaaa
      241 tacccatatt tttgtacata acgagagaaa aagtagtggc agctactaca taaggaattc
      301 cttgtccaaa atacaggcta ggaatccatg cccatggtga aataggttta tttttcatat
      361 atattttttt atctactagt aattggcgtt aattaatttt cttcttcgtg taagattctt
      421 tcacagaggg acacagaggt ttcacagagg tacacagagt aatatgtttt tctctcagtg
      481 taacttcgtg tttccttcgt gttcttcgtg aaataatagt atacaaagtg taacaaaaag
      541 actaatatac ggaaagtata tccactcctg aataataatg ctgcaaaatt tctttatacg
      601 aatatccttg ttcactcatt acagctgctc ctatctggca aagacctaca ccatgccccc
      661 agcctgcccc ataaaaaata aaattccttt catcatctcg ctcaacgatg aatgctgaac
      721 tatataaatg cgtatctgat aaccacttac gaatctctaa ttcttttcca attcgcatag
      781 ttctggttgt tcctacaatc tcaataattt ctaatcgacc cgattttcca cgtttaatgg
      841 gacgtatatc gtgtataact ccaaaatcaa tacccgatcg tttgtgtact aatgtcgata
      901 attcgtgttg actatacact ttcttccacc taaaaaagtc tgtagtttcc tgatcgaaat
      961 gaggcagaat ttgagacaaa atatgagcat cagaagtatt acaaaataca gttggtgatg
     1021 atttaatcca agatgttatt gctgactcag tacgtaagtc caactttttc tcttcctgtt
     1081 tttcatctac tattgattgt aaataagcaa cttgattgtt ttcccaacac gtagcatact
     1141 cttcggttac gcctccacaa cattttgaaa agcgtgcatc acacaagtta ttttgatata
     1201 ctaacacctc tccatatgtt tcttcaatag ctttttgcac ctgatttgta gttaccttcg
     1261 ctacgccctg ataccgttgg caatgatcgt cagcacacac atcaaatagt aaatgattgt
     1321 gatggtcata ccattttatc atcgtattcc cctcaatact caacgtttca gcctgacttg
     1381 aacagacttg ttgtatttgt gcaaaaagcc aactacgaga tataacagca tgagctttta
     1441 ataaggaaat agaagatgta gcactcattt cagatgaaat aacactcagc aaatactctt
     1501 caacaggtaa ggtattaatt aatacaatgt tgtcttgttc aacaacaaaa cgtaatgtac
     1561 ccttaaacgt ttggttttct tttcgttgcc aatgaaaatc cacaccaatt actacatcat
     1621 aaaccaaaaa cgtacctaca ttagcatcgt tcggctcaaa acacaattca ttatacagtt
     1681 gattattaaa caaaatttta ccattgacac acgaaactat gcattcgcct tgcacttgct
     1741 c
//
LOCUS       DAOSMY010000327         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_69093_length_1736_cov_2.126115, whole genome shotgun sequence.
ACCESSION   DAOSMY010000327 DAOSMY010000000
VERSION     DAOSMY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69093_length_1736_cov_2.126115"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PLD76_06715"
     CDS             <1..132
                     /locus_tag="PLD76_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOU27726.1"
                     /translation="IGFDDPSHAVGSEEFIWSEFIRVRDEIKEGFYAFYTEKIKPQL"
     gene            140..1189
                     /gene="arsB"
                     /locus_tag="PLD76_06720"
     CDS             140..1189
                     /gene="arsB"
                     /locus_tag="PLD76_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOU27727.1"
                     /translation="MNKEAEKMSFFDRYLSMWVAICIVVGIAIGKLLPIVPETLGQLE
                     YANVSIPIAVLIWIMIFPMMLKIDFKSVVDAVKMPKGLTITLVVNWLIKPFTMFGIAW
                     LFFMVIYSLWIPEDLAKEYLAGAVLLGAAPCTAMVFVWSHLTKGNPAYTLVQVAINDL
                     IIIVAFIPIVTLLLGVSGIVIPWNTLIMSVLLFVVIPLVLAYITRNWVIRKKGIAYFT
                     DVFIKKFSATTISGLLLTLVIIFTFQGDVILQNPLYILLIAVPLIIQTFFIFFVAYRW
                     AKAWKLPHDIAAPASLIGASNFFELSVAVAIVLFGLQSGATLVTVVGVLVEVPVMLAL
                     VKIANKTKHQFPSKQ"
     gene            1264..>1736
                     /locus_tag="PLD76_06725"
     CDS             1264..>1736
                     /locus_tag="PLD76_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOU27728.1"
                     /translation="MTLINKINIGIDAGSTTVKIVVTNQHHALLYKEYVRHKADVAGT
                     IVTSLLPLLHTIGNKDVSVTITGSAGMGIAERCSLPFVQEVVASCELIQTLYPTVKTL
                     VDIGGEDSKMIFFSPNHAPDMRMNGNCAGGTGSFIDQIATLLGVGVHELDELAQAA"
BASE COUNT          468 a          293 c          379 g          596 t
ORIGIN      
        1 attggtttcg acgatccaag tcatgctgta gggagcgagg agtttatttg gagcgaattt
       61 atacgtgttc gcgatgaaat aaaagaggga ttttacgctt tttatacgga aaaaattaaa
      121 ccccaattgt aaggttgtca tgaacaaaga agctgaaaaa atgtcttttt ttgaccgtta
      181 cctctctatg tgggtggcaa tctgtattgt ggttggtatt gctataggca aattgttacc
      241 aatagtaccc gaaaccttag gtcaattaga gtacgccaat gtgtctattc ccattgctgt
      301 gcttatttgg ataatgattt ttccgatgat gctcaaaatt gactttaaaa gtgtggtaga
      361 tgcagtcaaa atgcccaaag gtcttaccat taccttggtg gtaaactggc tcatcaaacc
      421 atttactatg tttggtattg cctggttgtt ttttatggtt atttattcgc tgtggattcc
      481 cgaagacctt gcaaaagagt accttgcagg tgcggtattg cttggtgcgg ctccgtgtac
      541 ggcaatggtg tttgtgtgga gtcatttaac caaagggaat cctgcttaca ccttggtaca
      601 agtagccatc aacgatttaa tcattattgt agcttttatt cctattgtaa cgcttttatt
      661 aggcgtttct ggtatcgtta ttccgtggaa tacgctcatt atgtcggttt tgttgtttgt
      721 agtcattccc ttggtgcttg cttatatcac acgcaattgg gttatccgca aaaaaggtat
      781 cgcctatttt actgatgtgt ttatcaaaaa gttttctgcc actaccataa gtggtttatt
      841 gcttactttg gtaatcattt ttacatttca aggggatgtg attttgcaaa atcctttgta
      901 tattttattg attgcagtac ctcttattat ccaaacattc tttattttct ttgtggcata
      961 tcgatgggct aaagcatgga aactaccgca cgatattgct gctccagcat cgcttattgg
     1021 tgctagtaat ttttttgaac tatcggtggc agtagccatt gtgttgttcg gattgcagtc
     1081 gggagctact ttggtaacag tagtaggcgt attggtagaa gttcctgtaa tgctggcatt
     1141 ggttaaaatt gctaataaaa caaaacatca gtttccaagc aagcaataaa aagaatatct
     1201 tgctattttt ttgtacattt gtggcgattt ttggaaaata tgccatatcc acaaaaaagg
     1261 aagatgacgt taatcaacaa aattaatata ggtattgatg ctggttcaac tacagtaaag
     1321 attgttgtaa ccaaccagca tcatgctttg ttgtataaag aatatgtgcg gcataaagcc
     1381 gatgttgctg gaacaattgt aacttcatta ttgccactat tgcataccat tggcaacaaa
     1441 gatgtttctg taaccattac cggttcggct ggtatgggta tagctgagcg ttgtagtttg
     1501 ccttttgtgc aagaagtagt ggcatcttgc gaactgattc aaaccttata ccctactgta
     1561 aaaacattgg ttgatatagg cggagaagat tctaaaatga tatttttttc gcctaatcat
     1621 gctcctgata tgcgtatgaa tggcaattgt gctggaggaa cgggttcgtt tatcgaccag
     1681 atagcaacgc tgcttggtgt aggtgtacac gaattagatg aattggcaca agctgc
//
LOCUS       DAOSMY010000328         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_69419_length_1729_cov_4.221027, whole genome shotgun sequence.
ACCESSION   DAOSMY010000328 DAOSMY010000000
VERSION     DAOSMY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69419_length_1729_cov_4.221027"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1729
                     /locus_tag="PLD76_06730"
     CDS             <1..>1729
                     /locus_tag="PLD76_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOU27729.1"
                     /translation="SPSYKRKINGIIHDESASGKTIFIEPAEVVETNNQIRELELQEK
                     REITRILVQCANFIRPMLPDLLHSYHFLGTIDFIRSKALLAIQLQATKPVIENNPHID
                     WQDTFHPLLYLSLRKQGKTIVPLSIKLNDQERILVISGPNAGGKSVCLKTVGLIQYMM
                     QCGLLVPMKESSTMGIFQRIFIDIGDEQSIENDLSTYSSHLTNMKFFAKHCQETTLLL
                     IDEFGTGTEPLIGGAIAEATLHVFNQQKAFGVITTHYTNLKHFATENEGVVNGAMLYD
                     RHLMQPLFQLEIGNPGSSFAIEIARKIGLPEHIIADATLKVGAEHIDFDKNLQDIARD
                     KRYWENKRQQIRLKEKKLEETTEQLRKELEQVTKQRKQVLKDAKNEASLLLQQANASI
                     ENTIRTIKQSQADKEKTAIARKKLEEYKQSINDTQQQDVLEQRLSAIKPLKANKEKSE
                     QTENKTLHVGDAVRIIDKETTGTIVSIHQKTAIIAIGQLKSTIKLDQLERISNKQQKQ
                     LQRHISYISQRTQDEMRERKLQFKQEIDVRGKRAEEALQIIRYYIDDAFIVNASRVRI
                     LHGTGTGALR"
BASE COUNT          625 a          364 c          321 g          419 t
ORIGIN      
        1 tttctccttc gtacaaacgc aaaattaatg ggattataca cgacgaatct gccagtggaa
       61 aaaccatttt tattgaacct gccgaagtcg tggaaacaaa taatcaaatt cgcgaattgg
      121 agctgcaaga aaaacgcgaa attacccgca tcttggtgca atgtgccaat tttattcgtc
      181 caatgttacc cgatttactt cattcgtatc attttttagg caccattgat tttattcgct
      241 ccaaagcact gctagccatt cagctacaag cgacaaaacc tgttatcgaa aacaacccgc
      301 acattgattg gcaggatacg tttcatccct tgctttactt atcacttcgc aaacaaggga
      361 aaacgattgt tccactaagt atcaaactaa acgaccaaga acgtattttg gtcatttctg
      421 gaccgaatgc aggcggcaaa tcggtatgtt taaaaacagt aggtcttatt cagtatatga
      481 tgcaatgtgg cttactagtg ccgatgaaag aatcaagcac tatgggtatc tttcagcgaa
      541 tttttattga tattggcgat gaacaaagta ttgaaaacga tttaagtaca tacagttcgc
      601 atttaacgaa tatgaagttt tttgccaaac actgccaaga aactactctg ctactcatcg
      661 acgaatttgg cacaggtact gaaccgctta ttggaggtgc aatagcggag gctaccttac
      721 acgtatttaa ccaacaaaaa gcttttggtg taattactac ccattatacg aatttaaaac
      781 actttgccac cgaaaatgaa ggcgttgtaa acggtgcaat gctctacgat aggcacctta
      841 tgcaaccatt atttcaattg gaaataggta atcctggaag ttcctttgcc atcgaaattg
      901 cacgtaaaat tggcttgccc gaacatatta ttgcagacgc tacgctcaaa gtgggggctg
      961 aacacattga ttttgacaaa aacctgcaag atatagctcg tgacaagcgt tattgggaga
     1021 ataaacgtca acaaatacgc ctgaaagaga aaaaactaga agaaacaaca gaacaacttc
     1081 gcaaggagct tgaacaagtt accaaacagc gtaaacaagt actgaaagac gctaaaaatg
     1141 aagcatcgtt gcttttacaa caagccaatg cgtctatcga aaataccatt cgtactatta
     1201 agcaatcaca agcagataaa gaaaaaactg ccattgctcg aaaaaagctc gaagaataca
     1261 aacaatccat caacgacaca cagcaacagg acgttttaga acaacgactt agtgccatca
     1321 aaccactaaa agcgaacaaa gaaaaaagcg aacagactga aaacaaaaca ctacatgtag
     1381 gggatgcagt acgcattatc gacaaagaaa caactggcac aatcgtaagt atccatcaaa
     1441 aaacagccat catcgccatt ggtcaattaa aatcgactat taaactcgat caacttgaac
     1501 gcataagcaa taagcaacaa aaacaactac aacgccatat tagctatatc agccaacgta
     1561 ctcaagatga gatgagagag cggaaactac aatttaaaca agaaattgat gtgcgtggca
     1621 aacgagcaga ggaagctcta caaattatcc gctactacat tgacgatgct tttattgtaa
     1681 atgccagcag ggtacgtata ttgcatggca caggaaccgg agccttacg
//
LOCUS       DAOSMY010000329         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_69627_length_1726_cov_2.528426, whole genome shotgun sequence.
ACCESSION   DAOSMY010000329 DAOSMY010000000
VERSION     DAOSMY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69627_length_1726_cov_2.528426"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..512
                     /locus_tag="PLD76_06735"
     CDS             351..512
                     /locus_tag="PLD76_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27730.1"
                     /translation="MWGSFVGACKKSHLSNPRTSGKVLDNKFEKDKLIFRINDDEFWV
                     MFFGNPQEE"
     gene            768..1346
                     /locus_tag="PLD76_06740"
     CDS             768..1346
                     /locus_tag="PLD76_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOU27731.1"
                     /translation="MGVLDMKRIIIFSIITLFFISISGIAQENVHPDATEEARAKWVT
                     KPDGTITVRGFTFKRFHVVGDGTIIDWKTLLVWQQEPTSEKIKWQEAMDYAKNLRLAG
                     YSNWRLPTKAEIEALLVGHLDADVGNGADKPGAWLARNGFKNIKDSWYWSSSGNDTDT
                     SVVWGVSMFYGDVTGGNKSYGFGVALCVRYIQ"
     gene            1348..>1726
                     /locus_tag="PLD76_06745"
     CDS             1348..>1726
                     /locus_tag="PLD76_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOU27732.1"
                     /translation="MIINIRSLLYMLRTKLLLITIFTLLIFAGLRAQENVHPDAMEEA
                     RAKWVTRPDGTITVRDFTFKRFHVVGDGTIIDWKTLLVWQQEPTFDKMNWQKAKDYAS
                     ELRLAGYSNWRLPTKAEIEAFLVG"
BASE COUNT          610 a          233 c          369 g          514 t
ORIGIN      
        1 aacagaaata tagaagagta tggaggtgaa aatttaatgt gtttgacatg ggaccggatg
       61 ctactattac tcttatgatt ttgaatatca tgacattaat taaaaagacc ataatgcgac
      121 aagtcataac aaacaggttc tctaaataaa tgagatttta gtgagccaga tgtacacatg
      181 ggatttatga taataaagat aggtgcagga gttattaggt atatctttta aagaagcagg
      241 aaaggaacag aaaatcagat cttttcggct ataattttag tgataaatat tcggaataaa
      301 attttgatga agagtttaaa caaatatgaa tgatataaat atggagaaga atgtggggga
      361 gttttgtagg ggcttgcaaa aaatctcacc taagcaatcc tcgtacatct ggaaaggttc
      421 tggacaacaa atttgaaaaa gataaactga tttttaggat aaatgatgat gaattttggg
      481 ttatgttttt tggcaatcct caagaagaat aactaagata tttacaacct gttatcataa
      541 caaacataaa atgtaattaa gtaagctgag aattgaggtg ctaagtacca aaacttatcg
      601 gcgatgtctt aattcaaaag caaatcttac attttactta ctattagagc tacctgttaa
      661 tgtttttttt ataagtaaaa caattttaat aaagtaacgc aaatttaatt gacaaaaaat
      721 actacacggt ttatggtttt ttaataacat acattctaat ataagttatg ggagtattag
      781 acatgaaaag gataattatt ttttcaatca taacgttatt ttttatttca atatccggta
      841 tagctcagga gaacgtacat cctgatgcta cggaagaagc acgggcaaaa tgggtaacaa
      901 aacctgatgg tacaattact gttcgtggtt ttacattcaa gagatttcac gttgttggag
      961 atgggacaat aatagattgg aaaacactac ttgtatggca gcaggaacca acatctgaaa
     1021 aaataaagtg gcaagaagcg atggactatg ctaaaaattt aagacttgca ggttacagca
     1081 attggagatt accaacaaaa gccgaaatag aagcattact tgtagggcat ttagatgctg
     1141 atgtaggaaa tggtgcagat aaacctggtg cgtggctggc acgtaatggt tttaaaaata
     1201 ttaaagattc atggtattgg tcgtcttctg gtaatgatac agatactagc gtcgtgtggg
     1261 gcgtgagtat gttctatggc gatgtaaccg gtggcaataa gagttacggc tttggcgttg
     1321 ctttatgtgt ccgctacatc cagtaatttg attataaata taaggagttt gctttatatg
     1381 ctaagaacga aacttttact tattacaata tttaccctgc tgatttttgc aggtttaaga
     1441 gctcaggaaa acgtacaccc tgatgcaatg gaagaagcaa gagccaaatg ggtaactaga
     1501 cctgacggaa ctataacggt aagggatttt acttttaaaa ggtttcacgt agttggtgat
     1561 gggacgataa tcgactggaa gactcttctg gtatggcagc aagaaccaac atttgataag
     1621 atgaactggc agaaagctaa ggactacgca tctgaactta gactagcggg ttatagcaat
     1681 tggaggttac ctacaaaagc tgaaatagaa gcatttcttg tagggc
//
LOCUS       DAOSMY010000330         1719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_69985_length_1719_cov_3.238582, whole genome shotgun sequence.
ACCESSION   DAOSMY010000330 DAOSMY010000000
VERSION     DAOSMY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69985_length_1719_cov_3.238582"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLD76_06750"
     CDS             complement(<1..363)
                     /locus_tag="PLD76_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27733.1"
                     /translation="MKKKLRSISFYLLLWMLSSCVTEPSSSSIPYAWVSFEIDVSTGG
                     VHSSLRRDMLGNMLIFSVSNPSILSSSSNAGYGFGGVIVVRGMDNELYAFDATCPHEH
                     TSDVVLASDGYFVVCPLCK"
     gene            516..>1719
                     /locus_tag="PLD76_06755"
     CDS             516..>1719
                     /locus_tag="PLD76_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU27734.1"
                     /translation="MKKLFVTLLSTTILGSLFIGCSTKASTQDNRDCTNIVQPDWSRN
                     AVIYEVNIRQFTEEGTFKAFQSHLPRLKELGVDILWLMPVHPVSEVNRKGELGSYYAV
                     QDYKAVNPEFGTLEDFKSLVDESHQLGMKVILDWVANHTGCDNVWLQSHPEWYKRDSI
                     GNFIAPYDWTDVYELDYTKNEVREAMADALEFWVKDYNIDGYRCDVAFLVPLDFWEMA
                     RKRLDAIKPVFMLAEAEKPELTVNAFNMVYNWPLKDVMNQIAKGKNAEKANYIHESLL
                     SNASPIKNAQDIDTLVALQDSVFPCDAYQMNFITNHDENSWDGTEHERMGKGANTFAV
                     LTYTLNGMPLIYTGQEVGFDRRFEFFKKDTPPNWQDNETVLFYKKLNQLKKSQPALNA
                     GKSGGKFIK"
BASE COUNT          618 a          296 c          306 g          499 t
ORIGIN      
        1 tttacacaat ggacatacca caaaataacc atcacttgcc aaaacaacat cagatgtgtg
       61 ttcgtgagga catgtagcat caaatgcata taattcatta tccatacctc ttacaactat
      121 tacaccccca aaaccataac cagcattgct tgatgaagac aatattgaag gatttgatac
      181 agaaaaaata agcatattac caagcatatc tctacgtaaa gaactatgaa cacctcctgt
      241 agaaacatct atttcaaaag aaacccacgc atacgggata cttgaactag aaggctctgt
      301 tacacaagaa gacagcatcc atagtaatag ataaaatgaa atacttcgca attttttttt
      361 cataacatgc aaagatacat acgtttacat ataaaactta tatgtccgta taaaaaacaa
      421 tgaaaaatta gatatttttt ggtttcgata taaatatatc gcatatttgt aacacaattg
      481 ccattaatat taactaatca aatgaatgtc tacttatgaa aaaattattt gtaacgttgt
      541 tatccaccac aattttgggt agcctattta ttgggtgctc taccaaagca tctacgcaag
      601 ataaccgaga ctgtaccaat atcgtgcagc cagactggtc gagaaatgct gtaatttacg
      661 aagtaaatat ccgtcagttt acagaagaag gtacatttaa agcttttcaa tctcatttac
      721 cacgtttaaa agaattaggg gtggatattt tgtggcttat gcccgtgcat ccagtatctg
      781 aagtaaacag aaagggagaa ttaggaagtt actatgctgt acaagattac aaagctgtca
      841 atcctgaatt tggcacatta gaagatttta agtcgttggt cgacgaatct catcaattgg
      901 gaatgaaagt aattcttgac tgggtagcaa atcatactgg gtgtgataat gtttggttac
      961 aatctcatcc agagtggtac aaacgagatt ctataggtaa ttttatagct ccatacgatt
     1021 ggactgacgt atatgaatta gactatacca aaaatgaagt gagagaagca atggctgatg
     1081 cattagaatt ttgggtcaaa gattacaata tagatggcta tcgctgtgat gtagcgtttt
     1141 tggttcctct tgatttttgg gaaatggctc gtaaaagact tgatgctatt aaaccagttt
     1201 ttatgctagc cgaagcagaa aaacccgaac taaccgtgaa tgcatttaat atggtatata
     1261 actggccact taaagatgtt atgaaccaaa tagccaaggg taaaaatgct gaaaaagcaa
     1321 actacataca cgaaagctta ttaagcaatg caagtccaat taaaaatgct caagacattg
     1381 acacacttgt ggctcttcag gactctgttt ttccttgtga tgcatatcaa atgaatttta
     1441 ttaccaacca tgatgaaaat tcgtgggatg gaactgaaca tgaacgcatg ggtaaaggtg
     1501 cgaacacatt tgctgtatta acctatacat taaatggaat gcctcttatt tatacaggtc
     1561 aagaagtggg tttcgatcgt cgttttgaat ttttcaaaaa agatactcct cccaattggc
     1621 aagataacga aactgtacta ttctacaaaa agctaaatca actaaaaaaa tctcaaccgg
     1681 ctttaaatgc agggaaaagt ggaggcaagt ttataaaat
//
LOCUS       DAOSMY010000331         1719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_69986_length_1719_cov_3.230769, whole genome shotgun sequence.
ACCESSION   DAOSMY010000331 DAOSMY010000000
VERSION     DAOSMY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69986_length_1719_cov_3.230769"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1719
                     /locus_tag="PLD76_06760"
     CDS             <1..>1719
                     /locus_tag="PLD76_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU27735.1"
                     /translation="ADFINILYSEFLVFDPENPRWENRDRFFLDPGHMSPMLYAVLSL
                     MGNYSMDDLKAFRQWGSITPGHPELDVERGVENTSGPLGQGHTMAVGAAIAERFLAAR
                     FGDWTSHKIYTFISDGGIQEEISQGAGRIAGSLGLSNLIMFYDANNVQLSTYVDDITD
                     EDTAKKYEAWGWSVISINGNDAHEIRMALKKANEETQKPTLIIGKTLMGKGSMDANGN
                     SFENKVSTHGQPLTAAGACIRKTIENLGGNADEPFQIFDDVAQLYASRKESLKKIVAQ
                     RKLVQAQWEKENPALALKFHSFYANELPNIDFASIVQKPDQATRVASATILGHFSEKI
                     ENMIVSSADLSNSDKTDGFLKHTKPIQKGDFTGAFLHAGVSELTMACIMNGLALHGGV
                     IAVCGTFFVFSDYMKPALRMSALMELPVKYVWTHDAFRVGEDGPTHQPIEQEMQVRLL
                     ESLKNHSGKNSMLVLRPADVEETTVAWKMALENIDSPTGLILSRQNIKNIPRSANPYQ
                     EAAQVAKGAYIVKKAMSTPDVVLLASGSEVATLFEAAQLLETRKKLAIQIVSAPSEGL
                     YMNQDAS"
BASE COUNT          560 a          276 c          382 g          501 t
ORIGIN      
        1 ggcggatttt attaatattt tatattctga attcttagtt tttgatccag agaatccacg
       61 ttgggaaaat agagatagat tctttttaga tcctggacat atgtcgccaa tgttgtatgc
      121 agtattgtca ttaatgggta actactcaat ggatgatttg aaagcattta gacaatgggg
      181 tagtattaca ccaggacatc ccgaattaga cgtagaaaga ggagtggaaa atacctcagg
      241 tccattagga caaggacata ccatggctgt aggtgcagct attgcagaac gttttttagc
      301 cgctcgtttt ggcgattgga catctcataa aatttacacc tttatttcag atgggggaat
      361 tcaagaagag atatcccaag gagcaggtcg tatagcaggt tcgctggggt tgagtaatct
      421 aatcatgttt tatgatgcaa ataatgtgca actgtctacc tatgtagatg atataacaga
      481 cgaagatact gctaaaaaat atgaggcttg gggttggagt gttatttcta ttaatgggaa
      541 tgatgctcat gaaattagaa tggctttgaa aaaagcaaac gaagaaacac aaaaacctac
      601 tcttattata ggaaaaacct taatgggtaa aggatccatg gatgctaatg gtaatagctt
      661 tgaaaacaaa gtgtcgacac atggtcaacc tttaactgcc gctggtgcat gtataaggaa
      721 aacaattgaa aatttgggtg gaaatgctga tgagccattt cagatttttg atgacgttgc
      781 ccaattatat gcttctcgca aagagagttt aaagaaaata gtagctcaac gtaagctcgt
      841 gcaagcacaa tgggaaaaag aaaaccccgc attagctctt aagtttcatt cgttttatgc
      901 aaatgaatta cctaatattg attttgcttc catcgttcaa aagccagacc aagcaactcg
      961 agttgcatca gctaccatat tgggacattt ttctgaaaag atagagaata tgattgtttc
     1021 ttcagctgat ttatccaatt ccgataaaac agatggattc ctaaaacaca cgaaacctat
     1081 acaaaaagga gattttactg gagctttttt acatgctggt gtaagcgaat taaccatggc
     1141 atgtattatg aatgggttgg ctttgcatgg tggtgttatt gctgtgtgtg gcacattctt
     1201 tgtgttttcg gattatatga aaccagcctt gcgtatgtct gcattgatgg aattgcctgt
     1261 aaagtatgtt tggacacacg atgcgtttag agtgggtgag gatggtccta cgcatcaacc
     1321 tattgaacaa gaaatgcaag tgcgtttgtt ggaatcgcta aaaaatcata gtggaaaaaa
     1381 ttcgatgttg gtactaagac ctgctgatgt agaagaaaca acagtggctt ggaaaatggc
     1441 attagaaaat atcgattcgc ctacaggact tattttgtca agacaaaaca taaaaaacat
     1501 accaagaagt gctaatccgt atcaagaagc tgctcaggta gcaaaaggag catatatagt
     1561 taaaaaagcg atgtcaaccc ctgatgttgt gttgcttgca agtggttcgg aagtagctac
     1621 tttgtttgaa gcagctcaat tactggaaac aagaaaaaaa ctagcgatac agattgtatc
     1681 tgcaccttcc gaagggttgt acatgaatca agatgcatc
//
LOCUS       DAOSMY010000332         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_70816_length_1704_cov_3.848393, whole genome shotgun sequence.
ACCESSION   DAOSMY010000332 DAOSMY010000000
VERSION     DAOSMY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70816_length_1704_cov_3.848393"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PLD76_06765"
     CDS             complement(<1..1024)
                     /locus_tag="PLD76_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27736.1"
                     /translation="MKFVIYVIIFFYVMLTNVYAISEVSEIVAAKDSLHSDTATVMPS
                     CPLIKESWFTIPMGYYQQETSVALGIVGGYYFPSTNLRKISSISGSVIYTFRNQFIIN
                     ANPRFYGGDGNWFFNGNVNLRYYPTYYYGIGNSNSIYNESYISKSFQLMLQSGYFIND
                     NWQIGPYVSLRGEKITVGESFENHKEAIFARYGSAGWNPYWVTGLGFFSTYDTRDNVF
                     YPQKTSVFIKTSVLNYFSILGSTFDVASFNGDFRCFLPTWFGQLIACQLYLDARLGGN
                     IPFQLLPTVGGSDNLRGFRERKYIDDMFVQLQTEYRIPIWNRLKATLFYSVGDVVSTD
                     NPSFRLK"
     gene            complement(1122..1679)
                     /gene="frr"
                     /locus_tag="PLD76_06770"
     CDS             complement(1122..1679)
                     /gene="frr"
                     /locus_tag="PLD76_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU27737.1"
                     /translation="METKQYLTAAIEKMETTLLFLDDALAHIRAGKANVRILDAVRVD
                     YYGSMVPLSNVATLTTPDARTIAIQPWEKGLIKEIEKAILHSDVGITPENNGEMIRLG
                     IPPLTEERRKMLVKQSKQEGEEAKISVRNVRRDVIDAIKKAVKDGLAEDMAKDAEAEI
                     QKIHDKFIKKIDDAVLAKEKEIMTI"
BASE COUNT          556 a          354 c          223 g          571 t
ORIGIN      
        1 tttttaatcg aaaagaagga ttatcagttg acacaacatc cccaacagaa taaaaaaggg
       61 ttgcctttaa tctattccat atcggtattc tatactctgt ttgtaattga acaaacatat
      121 catctatgta ttttctctct ctaaatcccc gtaaattgtc agatcctcca acagttggca
      181 acaattgaaa tgggatattc cctcccaaac gtgcatccaa atacaattgg caagctatca
      241 attgaccaaa ccatgttggt aaaaaacacc taaaatcacc attaaatgaa gctacatcaa
      301 aagtactacc taaaatcgaa aagtagttca atacacttgt ctttataaat acacttgttt
      361 tctggggata aaatacatta tctcgcgtat cgtatgtaga aaaaaaacct aaacctgtca
      421 cccaataggg attccatcca gcactaccat aacgtgcgaa tatagcttct ttatgatttt
      481 caaaactctc acccacagtt atcttttctc ctctgagtga tacatatgga ccaatttgcc
      541 aattatcatt tataaaatag ccagattgaa gcatcaactg aaagctctta gatatataac
      601 tttcattata tattgaatta ctattaccta taccataata atacgtggga taatagcgta
      661 aatttacatt cccgttaaaa aaccaattgc catcgcctcc ataaaaacga ggattagcat
      721 ttataatgaa ttgatttcta aatgtgtata tcacactgcc agatatacta ctaattttac
      781 gcaagtttgt actaggaaaa taatatccac caacgatacc caatgcaaca ctcgtttctt
      841 gctgataata acccatagga atagtaaacc aactttcttt aataagcgga caagagggca
      901 taacagtagc tgtatcacta tgcaatgaat cttttgcagc aactatctca gatacttctg
      961 aaatagcata aacattggta agcatcacat aaaaaaaaat aattacataa ataacaaact
     1021 tcattagaga ccttctttag gtgttgattt acgttcttct ggtataaaaa accattcctc
     1081 gttgttacaa agaatggtaa aatactcgtt tattttaaat attatattgt cataatctct
     1141 ttctctttag ctaatacagc atcatcaatc ttttttataa acttatcatg aatcttttgt
     1201 atctcagctt ctgcatcttt agccatatcc tctgctaatc catcttttac agcttttttt
     1261 atcgcatcaa tcacatctct acgaacatta cggacactaa tctttgcctc ttcgccttct
     1321 tgtttcgact gttttactaa cattttacgt ctttcctctg tcaaaggagg aatgcctaaa
     1381 cgaatcattt ctccattatt ttcaggagta atacctacat ctgaatgcaa aattgctttc
     1441 tcaatctctt ttattaaccc tttctcccat ggttgtattg caatagttct tgcatcgggg
     1501 gtggttaatg ttgcaacatt actcaatggt accattgaac cataataatc gactcttaca
     1561 gcgtctaaaa tacggacatt tgctttgcct gcacgtatat gtgccaaagc atcatctaaa
     1621 aacagaaggg tggtttccat cttctctatc gctgccgtta agtattgttt tgtttccatc
     1681 tttaagttat atttacgtcg ttat
//
LOCUS       DAOSMY010000333         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_71442_length_1694_cov_3.090299, whole genome shotgun sequence.
ACCESSION   DAOSMY010000333 DAOSMY010000000
VERSION     DAOSMY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71442_length_1694_cov_3.090299"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLD76_06775"
     CDS             complement(<1..452)
                     /locus_tag="PLD76_06775"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU27738.1"
                     /translation="MLLPYVHKDLIECGCDEAGRGCLAGAVYAAAVILPTDFCCPELN
                     DSKKLTEKQRYALRPYIEQHALAFAIGVVSESEIDEINILNASFLAMHRAIEGLPIQP
                     QHLLIDGNRFKNKTTIPHTCIVKGDGKYASIAAASILAKTYRDDYMST"
     gene            complement(458..1444)
                     /locus_tag="PLD76_06780"
     CDS             complement(458..1444)
                     /locus_tag="PLD76_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOU27739.1"
                     /translation="MKSVVYLALFSLLLFVSCQSNEQSTSSQKQETQDLIQNPFYNAD
                     SAYVFVANQVAFGPREPNTDAHKKCGDYMVATLQRFGAEVTEQRVQLKAYNNTILDAR
                     NIIGSFNPEQKTRIFLCAHWDSRPYSDNDTDAANYHTPVQGANDGASGVGVLLELARL
                     MQQVPPTIGVDIILFDAEDYGRPSFDKSADIGDSFCLGSQYWSRNPHKEGYKPKYGIL
                     LDMVGGKNPTFLQEYFSMLYAPDVVKMVWKKAHQLGYQDYFISQPGNPVIDDHYYINK
                     IANIPCIDIIHYSNQTGFVDTWHTIYDTMENIDKTTLGVVGRVVTAVVYSEK"
     gene            complement(1455..>1694)
                     /locus_tag="PLD76_06785"
     CDS             complement(1455..>1694)
                     /locus_tag="PLD76_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27740.1"
                     /translation="IVIAGALAIFNMMFVPIFISLFFSKWVVDSWLLYKASDFLGQKR
                     LVLYSLLLSLVYPFYVISVAFCSMICPIKWKNRNI"
BASE COUNT          560 a          333 c          286 g          515 t
ORIGIN      
        1 aatgtggaca tatagtcgtc tctatacgtt ttggcaagaa tagaagcagc ggcaatggat
       61 gcatatttac catctccttt tactatgcat gtatgcggaa ttgtggtctt atttttaaat
      121 cgattgccat ctattaataa atgttgaggt tgaataggca aaccctcaat agcacgatgc
      181 atggctaaaa aagaggcatt gaggatattt atttcatcaa tttcactctc agaaaccaca
      241 ccaatggcaa acgccaaagc atgttgctca atatatggac gtaaggcgta gcgttgtttt
      301 tcggttagtt ttttcgagtc gtttagttct gggcaacaaa aatcggttgg caaaattact
      361 gcagctgcgt atactgcacc agccaaacaa cctcttccag cttcatcgca accacattct
      421 atcaagtctt tatgtacata tggcaacaac atacacctta tttttctgaa taaacaacag
      481 ccgttacaac tctaccaact acacctaagg tcgttttatc aatattctcc atggtgtcgt
      541 agatagtatg ccaagtatca acaaaacccg tttgattact ataatgaata atgtcaatac
      601 agggaatatt ggctattttg ttaatataat aatgatcgtc aataacagga ttacctggct
      661 gtgaaataaa ataatcttga tatcctaatt gatgagcctt tttccacacc attttcacca
      721 catctggagc atagagcatc gaaaaatatt cctgcaagaa agtaggattt ttacccccta
      781 ccatatccag taaaatacca tatttaggtt tatagccttc tttatgcgga ttacgtgacc
      841 aatattgaga gcccaaacag aatgaatcgc ctatatcggc agacttatcg aatgagggtc
      901 taccataatc ttctgcatca aaaagaataa tatctacacc aattgttgga ggtacttgtt
      961 gcattaaacg agctagttca agcaatacgc ctacaccact cgctccatca tttgctcctt
     1021 gcacaggcgt atggtagtta gcagcatctg tatcattatc agaatacgga cgagaatccc
     1081 aatgtgcaca taaaaagata cgtgtttttt gttctggatt aaatgaccca ataatattac
     1141 gtgcatctag tattgtattg ttgtatgctt ttaattgcac cctttgctct gttacttctg
     1201 ccccaaaacg ctgtaaagtg gcaaccatat aatctccaca ctttttatgt gcatctgtat
     1261 tgggttctct aggtccaaat gccacttggt tggctacaaa tacataggca gaatccgcat
     1321 tataaaatgg attttggatg aggtcttgtg tctcttgctt ttgcgatgag gtagactgtt
     1381 cgttcgattg acatgataca aataagagca aagaaaataa tgctaagtat actactgatt
     1441 tcatagaatt ataattaaat gtttcgattt ttccatttta tggggcaaat catagaacaa
     1501 aaagccaccg atataacata aaaaggatag actaacgaga gaagtaaact atataaaact
     1561 aatctttttt gtcctaaaaa atcagacgcc ttatagagta accaactatc tacaacccat
     1621 ttactaaaga acaaagatat aaaaattggc acaaacatca tattaaaaat agctaaagca
     1681 ccagcaataa caat
//
LOCUS       DAOSMY010000334         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_71462_length_1694_cov_2.259915, whole genome shotgun sequence.
ACCESSION   DAOSMY010000334 DAOSMY010000000
VERSION     DAOSMY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71462_length_1694_cov_2.259915"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..785
                     /locus_tag="PLD76_06790"
     CDS             183..785
                     /locus_tag="PLD76_06790"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU27741.1"
                     /translation="MKTKKTFFKAFGVAIAVALLGASCTNVEPTPKPIPPSDKLPVSE
                     SFATNQGAFTIVDVTLPEGGTYVWKWNTNKYMKASAFISSAKASESWLISPSIDLSSA
                     TAPKLTFEHTGKYFTSNKLTEQTLWVSTNYTEGAPSTATWTQLTIPTWPSGNDWMFVS
                     SGAIDLSAYTANSNVRLAFKYASTTAGAATWEFKNLVVKE"
     gene            complement(893..1564)
                     /locus_tag="PLD76_06795"
     CDS             complement(893..1564)
                     /locus_tag="PLD76_06795"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOU27742.1"
                     /translation="MKKILFLTSVLVVWQLSLTAQNFYEFKELPYSYSALEPYIDSTT
                     METHYAKHHKGYYNNFVKAMQEGNFDNKPLEQVIQNISSYSVFIRNNGGGYWNHEFFW
                     NCMSPNKTQASNALTKAITDNFGSFENFKKEFSSAASTVFGSGWAWLIVTDSGKLKIV
                     KTANQDNPLMDAVADKGTPILAIDVWEHAYYLKHKNLRAAYVENFWQVINWQFVSEKY
                     ADAVK"
BASE COUNT          530 a          291 c          312 g          561 t
ORIGIN      
        1 gtttgctaat aaaaaattac caacaggtac gggtgatatt gttgccttag taggttggta
       61 taatgacaaa gatgaaaaca tagacccaac aaaaatttac catcaactta ccatccgttc
      121 gttgagtgat ttaactgggt ttacttttcc tgaataaata ataatttaaa aataggatag
      181 atatgaaaac aaaaaaaaca ttttttaaag cctttggagt agctattgct gttgcactcc
      241 ttggtgcatc gtgtacgaat gtagaaccaa ctcccaaacc tattcctcct agtgataaat
      301 tacctgtgag cgaatcgttt gctaccaatc aaggagcctt taccattgta gatgtaactt
      361 taccagaagg aggtacttat gtttggaaat ggaatacaaa taaatatatg aaagcctctg
      421 catttatcag ttctgctaaa gcatcagagt catggttaat ttcgccaagt attgatttat
      481 caagtgctac agctcctaaa ttgacatttg aacacactgg aaaatatttt acatcgaaca
      541 aacttacaga acaaacccta tgggtatcta ccaattacac agaaggagct ccttctacag
      601 ctacttggac acagctaaca ataccaacat ggccaagtgg caatgattgg atgtttgtat
      661 cttctggagc aattgattta tcagcttata ctgccaatag caatgtgcgt ttagccttta
      721 agtatgcaag tacaacagct ggtgctgcta cgtgggagtt taaaaatttg gttgtaaaag
      781 aataatagaa ctatttgtta gatagtacaa aaagagggtg aatcgtgcga ttcatcctct
      841 ttttattgag taattaatat tcagtttatc aatttgcggt tatatagatg tgctatttta
      901 ctgcatccgc atatttttct gatacaaatt gccaattgat aacttgccag aaattttcaa
      961 catacgctgc acgtaggttt ttatgtttca aataataggc gtgctcccat acatcaatgg
     1021 ctaagatggg tgttccttta tctgcaacag catccatcaa tggattgtct tggttagcgg
     1081 tttttacaat tttcagtttg ccactatctg ttacgataag ccatgcccat cccgaaccaa
     1141 aaacggtaga tgctgccgaa gagaattctt ttttgaaatt ctcaaacgaa ccgaaattgt
     1201 ctgtaatggc ttttgtgagt gcattgctgg cttgtgtttt gtttggcgac atacaattcc
     1261 agaaaaactc atgattccaa tatccccctc cgttgttacg aataaataca ctgtaagagg
     1321 aaatgttttg tattacttgt tcgagaggtt tattgtcaaa attgccctct tgcatcgctt
     1381 ttacaaagtt gttgtagtat cctttgtggt gtttagcata atgcgtttcc atggtggtgg
     1441 agtcgatata aggctctaat gcactatacg aatagggtag ttctttaaat tcatagaaat
     1501 tttgagctgt aagtgatagt tgccatacaa ctagtacact cgtaaggaat agtatttttt
     1561 tcatattttt attgtttaat tttacaaata taaacccaaa atgccctttc gtaattttag
     1621 ttaacaagtt ttagtgatat gcaatgaata taaacagaag agtttcttat taatcgtaga
     1681 gaaaaagcgg cttg
//
LOCUS       DAOSMY010000335         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_73668_length_1657_cov_3.051186, whole genome shotgun sequence.
ACCESSION   DAOSMY010000335 DAOSMY010000000
VERSION     DAOSMY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73668_length_1657_cov_3.051186"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..>1657
                     /locus_tag="PLD76_06800"
     CDS             97..>1657
                     /locus_tag="PLD76_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU27743.1"
                     /translation="MKPKTKKTILIGLWTLFASIIVGTVLLFFLIAKGVIGYIPEIEE
                     LQNPKNKFASEIYTADGEVLGRFFYGKDNRVAVNYTQISPYMIEALVATEDIRFFNHS
                     GIDARSLGRAIILRGLLGKASAGGGSTITQQLAKLLYSPNADNLFQRAMQKPIEWVIA
                     VQLERFYAKEEIIAMYLNQFDFLNNAVGIKSASQVYFNVAPNELNREQAALLVGMCKN
                     PSLFNPNRFPERAKNRRNTVLDQLYKYDYITKQEVDSLKSIPIKLNFQRVDHKLGLAP
                     YFREYLRQTMMADKPQRSNYMSWQMQKYKEDSLAWETNPLYGWCKKNKKPNGSNYNIY
                     ADGLRIYTTIDARMQRYAEEAVKEHIGDNLQKRFFNEKRGRSYAPFSRNITPEEMSSI
                     LTKAKRQSDRYRNMKKANYTNAEIDRAFAKKIPMSVFTWTGVRDTIMSPMDSIKWQKH
                     FLRCGFMSMDPYNGHVKAYVGGPNFAEFQYDMVTQGKRQVGSTIKPYLYTLAMEEGLT
                     PCQKVPNIPQTF"
BASE COUNT          577 a          265 c          308 g          507 t
ORIGIN      
        1 atattcatac ttttttgttt aaaaatagag tctttactag tcgtttgaaa aaaatactgt
       61 acctttgtgg gcaaaaattt atcaagaaac tactacatga aaccaaaaac taaaaaaacg
      121 attcttattg gactttggac actttttgcg tcaatcatag ttggtacagt attgctattt
      181 ttccttatag caaaaggtgt tattggttat ataccagaaa ttgaagagtt gcaaaatcca
      241 aaaaataaat ttgcatctga aatatacacg gcagatggag aggttttagg aagatttttc
      301 tatggtaaag acaaccgagt agcggtaaat tatacccaaa tttctcctta catgatagaa
      361 gcattggtag ctacagaaga tattcgtttt tttaaccatt ctggtattga tgcacgttct
      421 ttgggaagag ctattatttt acgaggttta cttggaaaag ccagtgcggg tgggggtagt
      481 actattacac aacagttagc taaattactt tattctccta atgcagataa tctctttcag
      541 cgtgctatgc aaaaacctat agaatgggtg atagctgtac aactagaacg tttttatgct
      601 aaagaggaaa taattgcaat gtatcttaac caatttgatt ttttgaataa tgccgttggt
      661 ataaaatcag cttcacaggt atatttcaat gttgccccca atgaactaaa tagagaacaa
      721 gcagctttgc ttgtaggaat gtgtaaaaac ccttcgttat ttaatccaaa ccgctttcca
      781 gaacgtgcta aaaataggcg taatacagta cttgaccaat tgtataagta tgattatatt
      841 actaaacaag aagtagattc attaaaatca attccaatta aattgaattt ccaacgagtt
      901 gatcataagt taggtttagc tccttatttt agagaatatt tgcgtcaaac gatgatggct
      961 gataaacccc aaagaagcaa ttatatgtcg tggcaaatgc aaaaatataa agaagactcg
     1021 ttagcctggg aaacaaatcc attgtatggt tggtgtaaaa aaaataaaaa acccaatggg
     1081 tcaaattaca atatttatgc agatggatta cgcatttata ctacgataga tgcacgcatg
     1141 caacggtatg cagaagaggc agtgaaagag catattggag ataatttgca aaaacgtttt
     1201 tttaacgaaa aacgtggacg ttcgtatgct ccattttctc gaaatattac tccagaagaa
     1261 atgtctagca ttttaacaaa agcaaaacgt cagtcagata ggtatcgcaa tatgaaaaaa
     1321 gccaattata caaatgctga aattgatcgt gcttttgcta aaaaaatacc gatgagtgta
     1381 tttacttgga caggtgttcg tgatactatt atgagcccaa tggattctat caaatggcaa
     1441 aaacactttt tacgctgtgg atttatgtct atggatccct ataacggaca tgtaaaagcc
     1501 tatgtaggtg gaccaaactt tgctgaattc caatatgata tggtaacgca aggaaaacgg
     1561 caagtgggtt ctaccatcaa gccttattta tatacactgg ctatggaaga aggcttgact
     1621 ccttgtcaga aagtgcccaa tattccgcaa acctttt
//
LOCUS       DAOSMY010000336         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_73957_length_1652_cov_4.361929, whole genome shotgun sequence.
ACCESSION   DAOSMY010000336 DAOSMY010000000
VERSION     DAOSMY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73957_length_1652_cov_4.361929"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(406..549)
                     /locus_tag="PLD76_06805"
     CDS             complement(406..549)
                     /locus_tag="PLD76_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27744.1"
                     /translation="MHTKASSIGGIFLSHFAMSTISQSILFLIANSENYKAMRFALPV
                     PEK"
     gene            518..1411
                     /locus_tag="PLD76_06810"
     CDS             518..1411
                     /locus_tag="PLD76_06810"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOU27745.1"
                     /translation="MPPIDDALVCIISPIHGFNYPKITLDFIRNFPKGNNRVVLMNTR
                     AGMKIGNFVTPGLTGIAFFVSSFILRNKGYKIVGQIPFDMPSNWLSIHPALNEKTVKY
                     LHRTNYNRVEKHAVNIFSGQRDFLAYRDVIQDILISPIAIAYYFIGRFILAKSFYATI
                     DCDSCGLCIKQCPVQAINLFHKHPFWTFKCESCMRCMNNCPKRAIETAHGLLIGVSIV
                     SSVGFTIVVERVLQLEIHSSFVSLTVWSVIFIAILWILYYIQHMLLKYRILSKIVSLL
                     SLTHYTCWGRYKSIPDKKWKE"
     gene            complement(1446..>1652)
                     /locus_tag="PLD76_06815"
     CDS             complement(1446..>1652)
                     /locus_tag="PLD76_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27746.1"
                     /translation="YIFYGSNSYGVYPFVNLYFTSNLSLAFSVFKPKILIFCKATKDF
                     LIVKTKCEFLDKIFSTSINVTNEG"
BASE COUNT          573 a          250 c          277 g          552 t
ORIGIN      
        1 cgttccaaag tacttcgttt acaggtttta cttcttcgtt gccacaatag catccttcgg
       61 tacgaatttc gttaactagt tgtaaaataa gtttttcatt aagtgtttgt tgggcaaata
      121 gccaagttgg taaaacaacg aatgccaata gccagataga tttgttcata ctttatgtta
      181 tttttttttg aaacgcaaat gtaacaaaaa atattattta ttagatacaa ttctactcaa
      241 taatatcaaa aatataccag taaattttga aacgtcccta aaacgcatta cttttgaaca
      301 aaaaatcaac ttaatggagg ttttttgaga ccgccgttag gtagaatata gaatatagag
      361 aaattaacaa caggcgatga atactattca gaaagttatc ctattttatt tttcaggaac
      421 aggtaatgca aaacgcattg ctttgtagtt ttctgaattt gcaatcaaaa acagaataga
      481 ttgcgaaata gttgacattg caaaatgtga taaaaatata cctccaattg atgatgcttt
      541 ggtatgcata atttctccga tacatgggtt taattatcca aaaattacat tggattttat
      601 tcgtaacttt ccgaaaggaa acaatcgtgt tgttttgatg aatacacgag caggaatgaa
      661 aatagggaat tttgtaactc caggcttaac gggaattgca ttttttgtat cgtcattcat
      721 attaagaaac aaaggctata aaattgtagg gcaaattcct tttgatatgc cttctaattg
      781 gctttcgata catccagcat tgaatgaaaa gacggtaaaa tacctccaca gaacaaatta
      841 caatagagta gaaaaacatg cagtcaacat tttctcaggt cagcgagatt ttcttgcata
      901 cagagacgta attcaggata tacttatttc gcctattgct atagcttatt acttcatagg
      961 cagatttata ttggcaaaat cattttatgc tacgatagat tgtgattcgt gtggtttgtg
     1021 tattaaacaa tgtcctgtac aggcaattaa cctgtttcat aaacatcctt tttggacttt
     1081 taagtgtgaa agttgtatgc gttgtatgaa taattgcccc aaaagagcca ttgaaacagc
     1141 acacgggtta cttataggag taagcatagt aagttctgta gggtttacaa tagtagtaga
     1201 acgtgttttg caattggaga tacactcttc atttgttagc ttgactgtat ggtcggttat
     1261 cttcattgca attttgtgga ttttgtatta catacagcat atgttactaa aatacagaat
     1321 actatctaaa attgtatcct tactatcatt gacacattat acatgttggg gacgttacaa
     1381 atcaataccc gacaaaaaat ggaaagaata actacagtcc acttgtggag gatagggtaa
     1441 tctctttacc cctcgtttgt aacattgatg cttgtactaa aaattttatc taaaaactca
     1501 catttagttt ttacaatcag aaaatcttta gtagctttac aaaaaattag tatttttggc
     1561 ttaaaaactg aaaaagctaa ggataaattg gaggtaaaat ataaattaac aaatggatat
     1621 acgccataac tattgctacc ataaaaaata ta
//
LOCUS       DAOSMY010000337         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_73973_length_1652_cov_3.370695, whole genome shotgun sequence.
ACCESSION   DAOSMY010000337 DAOSMY010000000
VERSION     DAOSMY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73973_length_1652_cov_3.370695"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLD76_06820"
     CDS             <1..431
                     /locus_tag="PLD76_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOU27747.1"
                     /translation="IPIEISTNDTTSFLCNAAVSACSNLGSKAIFTDTYSGLTARYLA
                     AYRGQNPIFAICYHERSTRLLALSYGVFPFFQDSGNDNTQQYIIKGLQFLLNEKWINI
                     NDTVCYLSGSFGIGFGTTFLEINTVKKIFESREKYILPNF"
     gene            675..1367
                     /locus_tag="PLD76_06825"
     CDS             675..1367
                     /locus_tag="PLD76_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOU27748.1"
                     /translation="MKQIHFTPQMKLASLILHNFKLLPVITRFGIPLGFGEKTVAEVC
                     SHYAVPTDFFLLVCTIYTQDDYMPTPSEIQTIDIHTLLSYLSNSHQYYLTERIVEIEQ
                     SIHAMNQSCSNKHYSIIETFFDGYKKEVIKHLEYEDSSVFPYVYKLIAGEKTPGFGIQ
                     QFEKNHTNIEEKLGDLKNIIIKYVPNDCPSRERNKLLYDIFLFEEDLNRHTLIEDKVL
                     IPYVESIEKSQN"
     gene            1391..>1652
                     /locus_tag="PLD76_06830"
     CDS             1391..>1652
                     /locus_tag="PLD76_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27749.1"
                     /translation="MTTQTNQRIIIIEPSTIVFAGLQSIIERMHIAESIYHVTELDTI
                     TQKLAQLRADIIIVNTAVFGFHKNLSIKSIFKDCTHASIIAFV"
BASE COUNT          571 a          330 c          249 g          502 t
ORIGIN      
        1 atattcctat tgaaataagc accaacgaca ccacatcttt cctatgcaat gctgctgttt
       61 cagcatgctc taacttaggg tcaaaagcca tttttactga cacctacagc ggacttacag
      121 ctcgctattt ggcggcgtat cgtggtcaaa acccaatttt tgctatctgc tatcacgaac
      181 gatctacccg attattagct ctttcctatg gagtttttcc attttttcaa gatagtggta
      241 acgacaacac gcaacaatac atcatcaaag gattacagtt tctacttaac gaaaaatgga
      301 ttaacatcaa cgatactgta tgttatttaa gtgggagctt tggtatcggt tttggcacta
      361 ccttcctcga aataaatact gtaaagaaaa tatttgaatc acgagaaaaa tacatcttac
      421 caaacttcta aaatcactac tttacaaaca aaactagata gacgttttag aggataaaag
      481 agaatctttt ttgctacaaa caaactagat aaactataat acaaaggtat gcatattccg
      541 cgtaggatat atctccatta ctgactagtg gttcgtttac ctacaatttt ctgattgcaa
      601 gcggacgatt tctaaaaaaa acgcctatct ttgtatgagt taattgccta cataatatac
      661 ttattccttt gccaatgaaa cagatacatt ttactccaca gatgaaatta gctagcctta
      721 ttttgcataa tttcaaactg ttgcccgtaa ttacccgatt tggtattcct ctcgggtttg
      781 gcgaaaaaac cgtagctgaa gtgtgtagcc actatgctgt tccaacagat ttttttctac
      841 tcgtatgcac tatttatacg caagacgatt atatgcctac acctagcgaa atacaaacaa
      901 ttgacataca cactttgctc tcgtatctat ccaattcgca ccaatactat ctaactgaac
      961 gcattgtaga aatagaacaa tctatacatg caatgaacca atcctgctcc aataagcatt
     1021 atagtattat cgaaactttt tttgatggat acaaaaaaga agtaatcaaa cacctcgaat
     1081 acgaggatag tagcgttttt ccttatgtgt ataaactgat tgcaggagaa aaaacaccag
     1141 gttttggtat ccaacaattt gaaaaaaacc acaccaatat tgaagagaaa ctaggggact
     1201 taaaaaacat tatcattaaa tatgtaccca acgattgccc ttcgcgtgaa cgcaataaat
     1261 tactatacga tattttctta ttcgaagaag acctcaaccg acatacattg atagaagata
     1321 aagtgttaat cccatatgtt gaaagcatag aaaaaagtca aaactaagtg agtagttact
     1381 acatgccatt atgacaacac aaacaaacca acgcattatc attatcgaac cttctactat
     1441 tgtgtttgct ggattgcaat caattataga acgaatgcac atagcggagt caatctacca
     1501 cgttacagaa ttagacacaa ttactcaaaa actagctcaa ctgcgtgcag atattattat
     1561 tgtgaataca gctgtgtttg gatttcataa aaacttatct atcaaatcta tttttaaaga
     1621 ttgtacgcat gcatctatca ttgcttttgt ca
//
LOCUS       DAOSMY010000338         1625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_75696_length_1625_cov_2.538217, whole genome shotgun sequence.
ACCESSION   DAOSMY010000338 DAOSMY010000000
VERSION     DAOSMY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75696_length_1625_cov_2.538217"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLD76_06835"
     CDS             <1..377
                     /locus_tag="PLD76_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27750.1"
                     /translation="MLLAMGRRNDLPHFFELIHKKTAVPHIAILFTSAIILLITLFGS
                     FPFVVRTATFTILLYYTIANAAAIRQPRIEQIYGSIIPWLGLIGCIVMAFSLPCMVIV
                     SGFGLLLIGFIARYIYTNIVAR"
     gene            643..>1625
                     /gene="pyk"
                     /locus_tag="PLD76_06840"
     CDS             643..>1625
                     /gene="pyk"
                     /locus_tag="PLD76_06840"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOU27751.1"
                     /translation="MSKATKIIATISDKRCDVDFLRQLYNEGMNVVRLNSAHLDLEGF
                     LRIINNTREVSNQIAILMDTKGPEVRTTIVQNNSIDIKTGDKIKISGNPDKISTHEHI
                     YVNYPFFVRDLNVNDDILFDDGEIDLKVIEKTTDYLLCEAQNDGMLGSRKSVNVPGVR
                     INLPSLTERDKQNILIAIENDIDFIAHSFVRNAQDIKDIQAILDQHNSPIKIISKIEN
                     QEGVDNINEILEQTYGVMIARGDLGIEVAQEKIPGIQRRLIRKCVQAKKPVIVATQML
                     HTMIHNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVEAVRTMATIA"
BASE COUNT          559 a          320 c          277 g          469 t
ORIGIN      
        1 gcatgctttt ggcaatgggt cgccgcaatg atttgcctca tttttttgaa ttgattcaca
       61 aaaaaacagc agttcctcat atagccattt tattcacttc ggcaattatt ttgttaatca
      121 ctctattcgg ttctttccca tttgttgtac gtacagccac tttcaccatt ctattgtatt
      181 acaccatagc taatgcagct gctattcgtc aacctcgcat tgaacagata tatggctcta
      241 taataccttg gttaggactg atagggtgta tcgtaatggc tttttctctc ccatgtatgg
      301 ttattgtttc tggctttggg ttattgctta ttggttttat tgcaagatat atatatacca
      361 atatagtagc aagataaaaa atggggaata gattctattc atataatcga aagcaattca
      421 tcttcaatat aaaagcaaaa tacaccgttt taagacaaat taactctaat aaatatcatt
      481 tctagaaaat ttgtcgcgtt tttcagtttt ttattgatat tatttgtatt tttgcatcca
      541 aaaaaaaaga aagcagcaat aactctataa ttacccttta gtatacactg tttttctttt
      601 aaaaaagaat aaaaattccc cctaatttaa taacaagctt ttatgtcaaa agccacaaaa
      661 atcatagcca ctatcagcga caagcgttgc gatgtggatt ttctacgcca attatacaac
      721 gaaggaatga atgttgtgcg gttaaactca gcacacctcg acttggaggg atttttacgc
      781 atcataaaca ataccagaga agttagcaac caaattgcca tactgatgga taccaagggt
      841 cctgaagtac gaacaaccat tgtgcaaaac aatagcattg atataaaaac aggggataaa
      901 atcaaaattt caggcaatcc agataaaata tctacccacg aacatattta tgttaactat
      961 ccgtttttcg ttcgtgactt gaatgtaaac gacgatattt tgttcgacga tggtgaaatt
     1021 gacctaaaag taattgaaaa aacaactgat tacctactat gcgaagccca aaacgatggt
     1081 atgctaggta gccgaaaaag cgtaaacgta cccggtgtac gcatcaattt accctcatta
     1141 accgaacggg acaaacaaaa catcttaata gcaattgaaa atgatatcga ttttattgcc
     1201 cactcttttg tacgcaatgc acaagatata aaagacattc aagctatact cgaccaacac
     1261 aacagtccaa taaaaattat ctcaaaaatc gaaaaccaag agggggttga taatatcaac
     1321 gaaatattgg aacaaacgta tggggtaatg attgctcgtg gtgatttggg cattgaagta
     1381 gctcaagaaa aaatacccgg aattcaacgc cgcttaatac gcaaatgtgt acaagccaaa
     1441 aaaccagtga ttgtagctac gcaaatgcta cacaccatga tacacaatcc tcgcccaaca
     1501 cgtgccgaag taactgatat tgccaacgct atctactacc gcacggatgc gttaatgctg
     1561 agtggcgaaa cagcttatgg caaatacccg gtagaggcgg tacgcaccat ggcaacaata
     1621 gccaa
//
LOCUS       DAOSMY010000339         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_77227_length_1601_cov_2.436611, whole genome shotgun sequence.
ACCESSION   DAOSMY010000339 DAOSMY010000000
VERSION     DAOSMY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77227_length_1601_cov_2.436611"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PLD76_06845"
     CDS             <1..1007
                     /locus_tag="PLD76_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOU27752.1"
                     /translation="AAFIMDKIMHKMGLHGKSFVPLLMGFGCNVPAILATRTIESKNS
                     RMITMLATPFMSCSARLPVYVLFAGAFFPNSASLVMLGLYLIGMLMAIISARLFKRFL
                     FNTEDVPFVMELPPYRMPTARATLRHMWEKSQQYLRKMGTIILFASIIIWALGYFPLA
                     ETRTPQLDDKINQLTALPTTEETNTQLQKLQWERNTQQQKTSYIGKIGNTILPIIKPL
                     GFNWEMGIGLIAGLPAKELVVSTLGVLYTNNATDENSAELSYKLQHVTDASGKQQITP
                     LIAFGYMIFILLSFPCIATIIAIKNESGSWKWGLFSIVYSTSLAWVCAFLIFQLGSLL
                     "
     gene            1142..>1601
                     /locus_tag="PLD76_06850"
     CDS             1142..>1601
                     /locus_tag="PLD76_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27753.1"
                     /translation="MKKITTLCIALLVASITALAATKTTETFELAGKTRTYLQYIPSS
                     YNAETPSKVLILLHGLNGNMNFFENQGYDLATMADLTNTIILSLQALDEQDTEVYDFI
                     TDIGTYQATSFTTTGVWGAGVSVPVSSFPRDAQFFISITYPSIAAAGKVKL"
BASE COUNT          486 a          348 c          307 g          460 t
ORIGIN      
        1 gtgctgcgtt tattatggat aagattatgc acaaaatggg cttgcacggc aaatcgtttg
       61 tgcctttgtt aatgggtttt gggtgcaacg tgcctgccat tttagctaca cgcactatcg
      121 aaagcaaaaa tagccgcatg ataaccatgc tagctacacc ctttatgtcg tgtagtgctc
      181 ggctaccggt gtatgtgttg tttgcggggg catttttccc taacagtgcc tcactcgtaa
      241 tgcttggatt atacctcatt ggcatgctca tggcaattat atcggctcgt ttgttcaaac
      301 gctttttatt caacaccgaa gatgtgcctt ttgtaatgga attgccacca taccgaatgc
      361 ctacggctag ggctactcta cgccatatgt gggaaaaatc gcaacaatac ttgcgtaaaa
      421 tgggtaccat catcttgttt gcatctatca ttatctgggc tttagggtat tttcccttag
      481 cagaaacgcg tacaccgcaa ctagatgata aaatcaacca actaacagca cttcccacca
      541 ccgaggaaac caacacgcaa ctgcaaaaac tgcaatggga acgtaacacg caacaacaaa
      601 aaacgagtta tattggtaaa attggcaata cgattctccc tattatcaaa cccctgggtt
      661 ttaattggga aatgggaatt ggtcttattg ctggactacc tgccaaagag ctagttgtta
      721 gcacattggg cgtattatat accaacaatg caaccgacga aaattcagcc gaactatcgt
      781 ataaactaca acatgtaacc gatgcctcgg gcaagcaaca aattacaccg cttatcgctt
      841 ttggctacat gatttttatt ctgttgagtt ttccgtgcat cgccactatc attgctatta
      901 agaacgaatc gggctcgtgg aaatggggat tattctctat tgtgtacagc acatctttgg
      961 catgggtatg tgcatttctc atttttcaat tagggagttt gttatagaaa aaaaataatc
     1021 ttaaaaaaag tagtaatttc taaaacttta ttacctttgc atagtaaact aacttagtat
     1081 aaactactta tgcgtttgca aatacaataa gcgtgtaaga caaaaaaaac aaaaaaactg
     1141 tatgaaaaaa atcactacac tttgcattgc cctattggta gcatcgatta cagcattggc
     1201 tgctactaaa accaccgaaa cctttgagtt agcaggcaaa acacgcacct acctgcaata
     1261 catccctagt tcctacaatg ccgaaacacc tagcaaggta ttgatcctgt tacacggact
     1321 gaatggcaac atgaattttt tcgaaaacca aggctacgac ctagcgacta tggcagatct
     1381 taccaacacc attattttat cgttgcaagc cttagatgag caagataccg aagtatatga
     1441 ttttataaca gatataggaa cttatcaagc tacctcgttt accactacgg gcgtatgggg
     1501 tgctggtgta agtgttcctg taagcagctt tcctcgagat gctcaatttt ttatttccat
     1561 tacgtacccg tccatcgcag ctgcaggaaa agtaaaactc a
//
LOCUS       DAOSMY010000340         1587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_78191_length_1587_cov_3.139687, whole genome shotgun sequence.
ACCESSION   DAOSMY010000340 DAOSMY010000000
VERSION     DAOSMY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78191_length_1587_cov_3.139687"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1297
                     /locus_tag="PLD76_06855"
     CDS             2..1297
                     /locus_tag="PLD76_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU27754.1"
                     /translation="MLAAIVVVFLGIYFLPLESVVFATAIDATLDLSRWYAREHVILC
                     LLPAFLIAGVIAVFVSQGSVIKYFGAKAKRWVAYTVAAVSGTILAVCSCTILPLFSSI
                     HKRGAGLGPAVAFLYSGPAINILAIILTARILGFEMGVARVIGAVVFSVVIGLIMAFI
                     YRKEEKVKAQEQLSFPDVPEKRPLWQTSLHFFTLVFILVFANWGRPAEGDTTSFWFYL
                     WQYKWHITGVFGLVLVYSMIRILKLSSWQVLLGAVVTVLAAFFIGNPLVVMVVAIAAV
                     SSIALLDKSEDTDNKEWILSSWGFTKQIIPLLAIGVVIAGFLLGSTHDDVSIPGIIPN
                     EWVAWAVGGNSILSNFLASIVGAFMYFATLTEVPIVQGLLASGMGKGPALALLLAGPS
                     LSLPNMLVIRGVMGTQKTIVYVAIVVVLSTIAGVVFGSL"
BASE COUNT          401 a          274 c          352 g          560 t
ORIGIN      
        1 aatactagct gccattgtcg tagtattttt aggtatttat tttttacctc tcgagagtgt
       61 tgtttttgct acggctattg atgctacgct cgatttatcc cgatggtacg cacgcgagca
      121 tgttattttg tgtttgttgc cagcgtttct tattgcgggt gttattgcgg tatttgtaag
      181 ccaaggttcg gtaattaagt attttggagc caaagccaaa cgctgggtag catatacagt
      241 agctgccgta tcgggcacta ttttagccgt ttgctcgtgt actatattac ccttattttc
      301 tagcattcat aagcgaggag caggcttagg tccagcagta gcatttttgt attcaggtcc
      361 tgcaatcaat atcttagcca ttattcttac ggcacgtatt ttgggtttcg aaatgggcgt
      421 tgcacgtgta attggagcag tagtgtttag tgtggttatc ggattgataa tggcatttat
      481 ctaccgcaaa gaagaaaaag tgaaagcaca agagcaatta tcgtttcctg acgtacccga
      541 aaaaagacct ttatggcaaa catcgctgca tttcttcacc ttggtattta tcttggtatt
      601 tgccaattgg gggcgaccag ccgaaggcga tacaacgagt ttttggtttt atctatggca
      661 atataaatgg catattacag gggtatttgg tttagtgctc gtttattcca tgatacgtat
      721 tttaaaactc tcatcgtggc aagtgttgtt aggagctgta gttactgtat tggctgcttt
      781 ttttattggc aatcccttgg tggtaatggt ggtagcaata gcagcagtaa gttctattgc
      841 tttgcttgat aaatcggaag acacagacaa caaggagtgg attttatcta gttggggttt
      901 taccaagcaa attataccct tattggcaat tggggtagtg atagcaggat ttttgttagg
      961 ctctacacac gacgatgtat ccattccagg cattattccc aacgaatggg tagcgtgggc
     1021 agtaggtggt aattccattt tatcaaactt tttagcctct attgtgggag cgtttatgta
     1081 ttttgctaca cttaccgaag ttcccatcgt acaagggctt ttagcttcgg ggatgggcaa
     1141 aggtcctgcc ttggcattgt tgctcgcagg tccatcgttg tcgttgccca atatgttggt
     1201 aattagaggt gtaatgggca cgcaaaaaac tattgtgtat gtggctattg tggttgtatt
     1261 atcaactatt gcaggagttg tttttgggtc gttgtaaata gtttacaagt tcataaagtt
     1321 ctaaagttgc atatttgttt tctttcaact ctattgcctg aaattcaatt aatcttccta
     1381 ctcctcgttt gtaacgagga gtttatatat tgtcgtttgt aacgacaata atacgctaat
     1441 cttccctcag ttcagatttt gtaattgtat cctaaggcta agtatactga ataaaaaata
     1501 aaaatagata ccataaaaat acctatattt ggttacgtta tatggtaagc cctcttgaca
     1561 ttactcaatc aattgtccta ttttatt
//
LOCUS       DAOSMY010000341         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_78424_length_1584_cov_2.712884, whole genome shotgun sequence.
ACCESSION   DAOSMY010000341 DAOSMY010000000
VERSION     DAOSMY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78424_length_1584_cov_2.712884"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLD76_06860"
     CDS             <1..327
                     /locus_tag="PLD76_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27755.1"
                     /translation="KSYNAYNTVYAKFTTNRREILSAGVLSVRIIDASTNRILQHQNF
                     VGEYVWYTEWSSYSGDERALSDKQKRMTQLQPQLPPPHQDLFIEFTKPIYSQVLTFVR
                     SAYRNY"
     gene            362..1027
                     /locus_tag="PLD76_06865"
     CDS             362..1027
                     /locus_tag="PLD76_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27756.1"
                     /translation="MKKSRFVLLSLLLSLFTYNILAQHDAGYKKYIPSYDISVAIGDP
                     MLASFYTDGYFLFRPYYDPKPFSFDDYVFGPEEYAGNTYITPVISTKITYQAASWYKV
                     GGAFGYFGYYAKKHDVFTDAIVRRENTHVFLLMPEMMLLWLNESIIQLYSGAGLGIAI
                     TMKEKLFTNEPSKNGLELSLLPTYHLNIIGARFGKELFGFVEYGVGFKGMFSAGIGYK
                     FVK"
     gene            1048..>1584
                     /locus_tag="PLD76_06870"
     CDS             1048..>1584
                     /locus_tag="PLD76_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27757.1"
                     /translation="MKHLSVLYTLFLLFILFSFSSCIFENQREPQPTQSGKAIYDSWH
                     THMFTVLQGDILKALHFSYYLDTQESQKIAVEDSLFSLFKIRDLSNGVWGLFLGADKI
                     YDINTSTTSLYHVGTEWIFKRTVSSSNHPLFSNSEIAYTITCIADSTWNISAVGDGNP
                     FCYVSLQLQQLGPTQFDVS"
BASE COUNT          491 a          233 c          305 g          555 t
ORIGIN      
        1 aaaagttata acgcatataa taccgtatat gcaaaattta ctaccaatag aagagaaatt
       61 ttatcagctg gagtgttgag tgttcgtatc attgatgcat caactaatcg tattttgcaa
      121 catcaaaatt ttgttggaga atatgtatgg tataccgaat ggtcaagtta tagtggggat
      181 gaaagagctc tttcggataa acaaaaacgt atgacacagt tgcaaccaca actccctcca
      241 cctcatcaag atttgtttat agaatttaca aaaccaattt attctcaggt gcttactttt
      301 gtgcgttcgg cttacagaaa ttattaatac ttgaagtgta tgtatgcata aatatatagg
      361 gatgaagaag agtagatttg ttcttttaag cctgttatta agtcttttta cttacaatat
      421 actagctcaa catgatgcgg ggtacaagaa gtatattcct agctatgata tttctgttgc
      481 tataggcgat ccaatgcttg catcgtttta tacagatgga tatttcttgt ttagaccata
      541 ttacgacccc aaaccgttca gttttgatga ttatgtattt ggacctgaag aatatgcggg
      601 taacacatat attactcccg taatctcaac aaaaattacc taccaggctg catcatggta
      661 caaagtaggt ggggcatttg gttattttgg atattatgct aaaaagcatg atgtatttac
      721 ggatgctatt gtacgaagag aaaatacaca tgtcttttta ttgatgcctg aaatgatgtt
      781 gttgtggctc aatgagtcaa ttattcaatt atatagtgga gcgggacttg gaatagctat
      841 aacgatgaaa gagaagctat ttacgaatga accatcaaag aatggtttag agttgagttt
      901 gttgcctact tatcatctaa acattattgg ggcacgtttt gggaaggaat tatttggttt
      961 tgtagagtat ggagtcggtt ttaaaggcat gttttctgct ggtattggat ataagtttgt
     1021 aaaataattt aaattaaaca attagttatg aaacatttat ctgttttata tacattattc
     1081 cttttgttta tactgttttc attttcgagt tgtattttcg agaatcaacg cgagccacag
     1141 cctacgcaga gtgggaaagc tatatatgat agttggcata ctcatatgtt tactgtttta
     1201 caaggagata ttctcaaagc attacacttt tcttattatc tagatacaca agaaagtcaa
     1261 aaaatagccg tagaagatag tcttttctct ctgtttaaga ttagagattt gtcaaatggt
     1321 gtgtgggggt tgtttttggg tgcagataag atttatgaca taaatacatc aactacatca
     1381 ttgtatcatg ttggtactga atggatattt aaacgtactg tttcttcgag taatcatcct
     1441 cttttttcta attcagaaat agcatataca attacctgca tagccgattc aacatggaat
     1501 atttctgctg ttggagatgg gaatcctttt tgctatgtgt cattacaatt acagcaactg
     1561 ggaccaacac agtttgatgt ttct
//
LOCUS       DAOSMY010000342         1572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_79257_length_1572_cov_2.406724, whole genome shotgun sequence.
ACCESSION   DAOSMY010000342 DAOSMY010000000
VERSION     DAOSMY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79257_length_1572_cov_2.406724"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLD76_06875"
     CDS             complement(<1..214)
                     /locus_tag="PLD76_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOU27758.1"
                     /translation="MKERNKRLEAIKEIIAHTTISSQDNLLESLIDKGFNATQATLSR
                     DLKQLKITKILTDDGIYKYVIPFTNYA"
     gene            328..>1572
                     /locus_tag="PLD76_06880"
     CDS             328..>1572
                     /locus_tag="PLD76_06880"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOU27759.1"
                     /translation="MMETRDNFTGKIGIVLATVGSAVGLGNIWRFPYEAGQNGGGAFI
                     LIYLLCVFFLGFPLVMAEFAVGVKAKANVVGSFKLLAPNTKWFLVGIMGVVAGFMIMG
                     FYYVVTGWTAEYFWQAIQGNLLHKSSEEYKVMFDSFIASPIKPVIWTVIMIALVHIVI
                     INGVKNGIEKVSKVLMPMLFVLMIILAIHSLMLPNAMQGIRFMFEPKVENVTSYGILT
                     ALGQAFFSLSLGMGCLITYASYFGKDTHIPRIAFQVTLLDSLVAIVAGVVIFPAVFSF
                     GLQPDEGTGLVFVILPTIFQQMPFPIFWSSLFYLLLALAAFTSVISLHEVVTAFVKEE
                     FSYTRKKAVTLVSVAVLVLAVLASLSLTLLFPVIFDKTLFEWLDFITATILLPLGGVA
                     VSLFVGWYVDKRILLDVLQQEGT"
BASE COUNT          412 a          205 c          332 g          623 t
ORIGIN      
        1 ttgcataatt tgtaaatggg attacatatt tataaatgcc atcatcggtt aatatcttgg
       61 taattttgag ttgttttaaa tcgcgggata acgtagcttg agttgcatta aaacctttat
      121 caatcaacga ttcaaggagg ttatcctgac tgctaattgt cgtatgtgct ataatttctt
      181 ttatagcttc tagccgtttg ttacgttctt tcatttttaa ataaatatac aattattctg
      241 caaaaatata cataaaaaat gaatatgcaa tcgtttatac attttttttg taaatattat
      301 gtatttttgc agtcataata tagatacatg atggaaacaa gggataattt tacaggaaaa
      361 attggcattg tgcttgctac tgttggttca gcagttgggt tgggaaatat ttggcgtttc
      421 ccgtatgagg ctggtcaaaa tggaggaggt gcatttattt taatttattt gctgtgcgtt
      481 tttttcttag gatttccgtt ggtaatggca gaatttgctg tgggagtgaa agcaaaagcc
      541 aatgtggtag gttcgtttaa attactagct cctaatacga aatggttttt agttggtatt
      601 atgggcgttg tggcaggttt tatgattatg gggttttatt acgtagttac tggttggact
      661 gctgaatatt tttggcaagc aattcaaggg aatttgttac ataaatcgtc cgaagagtac
      721 aaagtgatgt ttgattcttt tatagcaagt cctattaagc ctgttatttg gactgtaatt
      781 atgattgctt tagtacatat tgtaataata aatggggtta aaaatgggat tgaaaaagta
      841 tcaaaagtat tgatgcctat gttatttgtg ttgatgatta ttttagccat tcattcgtta
      901 atgttgccta atgcaatgca gggaatacga tttatgtttg agccaaaagt tgaaaatgtt
      961 acttcgtatg ggatattaac ggcattagga caagcttttt tttcgttgag tttgggtatg
     1021 ggttgtttaa ttacgtatgc ctcatatttt ggtaaagata cacatattcc tcgcattgca
     1081 tttcaggtta ctttgctcga ttctttagtc gcaattgtgg ctggtgtagt aatttttcct
     1141 gctgtatttt cgtttggttt acagcccgat gaaggtacag gactagtttt tgttatatta
     1201 cctaccattt ttcaacaaat gccttttcct attttttggt caagtctatt ttatttgttg
     1261 ttagcactag ccgcttttac ttcggttatt tcgttgcacg aggttgttac ggcttttgtg
     1321 aaagaagaat tttcctatac tagaaaaaag gctgttacgc ttgtatctgt agcagtgttg
     1381 gtattagctg ttttagcttc gttatcttta accttattat ttcccgttat ttttgataaa
     1441 acgctcttcg aatggttaga ttttattacg gctactattt tgttgccatt aggaggggtt
     1501 gccgtgtcat tatttgttgg ctggtatgta gataagcgta ttctgttgga tgtattacaa
     1561 caagagggga ct
//
LOCUS       DAOSMY010000343         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_79361_length_1570_cov_3.673927, whole genome shotgun sequence.
ACCESSION   DAOSMY010000343 DAOSMY010000000
VERSION     DAOSMY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79361_length_1570_cov_3.673927"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLD76_06885"
     CDS             complement(<1..639)
                     /locus_tag="PLD76_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU27760.1"
                     /translation="MKQTDIVNLWYLSLLKWRERKIKTSNFILVLSFLVGIFTALAAF
                     FLKSAIHFIQHILTTNFSVNHENYLYLAYPIIGVFITTLFVRYVVKDDISHGVTRILY
                     AISKRKSRLKPHNNWTSLVASSITIGFGGSVGAEAPIVLTGSSIGSNLGKFFKMDQKT
                     LMLLVGCGAAGAIGGIFKCPIAGVVFTLEVLMLDMTMTSVVPLLISSVTATSI"
     gene            complement(655..>1570)
                     /locus_tag="PLD76_06890"
     CDS             complement(655..>1570)
                     /locus_tag="PLD76_06890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU27761.1"
                     /translation="LSEMNQSLGYIIEGFVALQKNETRKVNNSSVVGSIVDINNVLKK
                     YPVDVVIIAVDSITEDDIYQLMQSLWSKNIKIKIVPSLFEILTGSVKMNSIYAVPLID
                     LLEIQMSDFQLNLKRLFDILLSSMVLLIGLPFFVIIALRIKLDSKGEVLYKQERVGLH
                     GIPFMMYKFRTMYENAEQDGVPRLSCENDERITPFGRFMRKYRIDEFPQFYNVLKGEM
                     AIVGPRPERRYFVDQIIEHAPYYYRLFNVRPGITSWGMVKYGYANTIEKMIERAQFDI
                     SYIENMSLVIDSKILIYTIKTIVTGRGI"
BASE COUNT          551 a          315 c          203 g          501 t
ORIGIN      
        1 aattgaggtt gcagttacag acgatattaa caatggcacc acagatgtca ttgtcatatc
       61 taacatcaat acttctaacg taaaaacaac tccagctatg gggcacttaa aaatacctcc
      121 gatagctcct gctgctccac atccaacgag taacattaag gtcttttggt ccattttaaa
      181 aaattttcct aaattagaac caatcgaaga tcctgtaaga acaataggtg cctctgctcc
      241 aactgaacct ccaaatccaa ttgtgataga actagccaca agagatgtcc aattattatg
      301 aggtttgagt ctactcttac gtttggatat ggcgtacaaa atacgtgtta cgccatgact
      361 gatatcatcc ttcactacat aacgcacaaa aagtgtagta ataaaaacac caatgatagg
      421 atacgctaga tacaaataat tctcatgatt aacactaaaa ttagtggtta atatatgttg
      481 gataaaatgt attgcacttt tcagaaaaaa agcagctaaa gctgtaaaaa ttcctaccaa
      541 aaaactcaaa acaaggataa agttactcgt tttaattttt ctttctcgcc atttaagcaa
      601 cgataaatac cacaaattta caatgtctgt ttgtttcata tctttcctta ttcgtcaaat
      661 ccctcttcct gttacaattg tttttatcgt ataaatcaat atcttactat ctattactaa
      721 cgacatattc tcaatataag agatatcaaa ttgagccctt tcaatcattt tttctatggt
      781 gttggcataa ccatatttta ccatgcccca cgatgtaata cctggtcgca cattgaacaa
      841 acggtaataa tacggagcgt gttcgataat ttggtctaca aaatatctac gttctggacg
      901 aggtcctaca atagccattt ctcctttcag aacattataa aattgaggaa actcatcaat
      961 tctatattta cgcataaaac gtccaaatgg agtaatccgt tcatcatttt cacaagataa
     1021 acgtggtaca ccatcctgtt ctgcattttc atacatggtt ctaaacttat acatcataaa
     1081 aggaattcca tgtaaaccta cacgctcttg cttatacaat acttcccctt ttgaatctaa
     1141 tttaatgcgt aaagcaataa ttacaaaaaa tggcaatcca atcaacaaaa ccatactaga
     1201 aaggaggata tcaaataagc gcttcaaatt gagctgaaaa tcgctcattt gtatttctag
     1261 taaatcaatt aaaggtacag cataaatact attcattttc acactaccag ttaatatttc
     1321 aaacaaactt ggtacaattt ttattttaat atttttcgac cacaacgact gcatcaattg
     1381 ataaatatca tcttcagtaa tactatcaac agcaataata actacatcaa caggatattt
     1441 ctttaaaacg ttattgatat ccacaataga gccaactaca ctgctattat taacttttct
     1501 tgtttcattt ttttgtaatg ccacaaagcc ttctattatg taacctaaag attggttcat
     1561 ttccgaaagt
//
LOCUS       DAOSMY010000344         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_79519_length_1568_cov_3.087244, whole genome shotgun sequence.
ACCESSION   DAOSMY010000344 DAOSMY010000000
VERSION     DAOSMY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79519_length_1568_cov_3.087244"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1538
                     /locus_tag="PLD76_06895"
     CDS             222..1538
                     /locus_tag="PLD76_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOU27762.1"
                     /translation="MLTKPKNTTQLGFFITFDDQLNHEHPLFQLANHIRWQVFEDAFS
                     KHYSATQGKPAKPIRLMVSLLILKQLRNLSDESIVEQWSENAYYQYFGGEQIFRPEQP
                     CVATELVEFRKRIGTEGVELIFKESIRINGKDSNDDTLSGDTTVQEKNITYPTDDKLY
                     KKIIDKCLIIAEKEQIELRQSYKYTIKKLSIIQRLRRNKGGDLKARKASKKIKIIAGR
                     LVRKLSATALNKHSIDLALFTKVLAQKRSDSNKIYSLHEPHVKCYTKGKEHKKFEFGS
                     KVSILVTQKTGVIVGALNFNDTLHDSKTLPDAIAQYERLTGKQAKDIFLDRGYRGPKT
                     INQTNLHTPKPNSNITKTKRKNHRQRAAIEPTIGHLKHDYRMIRNYLKGAIGDEMNVM
                     LSAAAMNFKRVMNLWVLGLKFFIEKICLLIIKKFCCLFGLMLKPTF"
BASE COUNT          641 a          248 c          263 g          416 t
ORIGIN      
        1 attaacagtg taataatttt caacaaaagg cagctttaaa gttgcctttt ttattcccct
       61 ttaaatctta taccagttgc cagtatttca ttaattagca ataattagtc gaacctcaaa
      121 aaggactaca ctctaatgat attaacaaat taggttgaaa agatgttata aaaaactgca
      181 agaaaagaat aaaaatatag aaaaaacttg cagatttgta tatgctaaca aaacctaaaa
      241 acaccacaca acttgggttt ttcatcacct ttgatgacca gttaaaccat gaacatccat
      301 tatttcaatt ggctaatcat attcgatggc aagtttttga agacgctttt tcaaaacact
      361 acagtgcaac acaaggcaag ccagcaaaac caatcaggct aatggtatct ttattaattc
      421 taaaacaact tcgcaattta agtgatgaaa gtattgttga acaatggagc gaaaacgcct
      481 attatcagta ttttggaggc gaacaaatct ttagaccaga acaaccatgc gtagcaacag
      541 aactggtaga atttcgtaaa cgcataggaa ctgagggagt agagttgatt ttcaaagaaa
      601 gtatccgcat aaatggaaag gattcaaacg atgatacatt aagtggagat acaacagtac
      661 aagaaaaaaa catcacttat ccaacagatg ataaattgta caaaaaaatt attgacaaat
      721 gcctgatcat agcagagaaa gaacaaattg aacttcgtca aagctacaag tacaccataa
      781 aaaagcttag tattattcaa cggctaaggc gtaataaagg aggagactta aaagcacgca
      841 aagcaagtaa gaaaataaaa ataatcgcag gtcgattagt acgaaaatta tcagcaacag
      901 cactcaataa acattcaata gatttagcat tattcacaaa agtattggca caaaaaagaa
      961 gtgatagtaa taagatttat agcctacatg agccccacgt aaagtgttac acgaaaggaa
     1021 aagaacataa gaaatttgag tttggaagta aagtatcaat acttgtgaca caaaaaacgg
     1081 gggtgattgt tggagcatta aattttaatg atacattgca cgattcaaaa acattacccg
     1141 atgcaatagc acaatacgaa cgtcttacag gaaaacaggc gaaagatatt tttttagata
     1201 gaggttacag aggaccaaaa acaataaatc aaactaactt acatacacct aaaccaaatt
     1261 caaacataac aaaaacaaag cgtaaaaatc atagacaaag agctgcaatt gagccaacaa
     1321 tagggcatct aaagcacgat tatagaatga tacgtaacta cctaaaaggt gctattggag
     1381 atgaaatgaa cgttatgtta tctgctgcag ctatgaattt taaaagggtt atgaatctct
     1441 gggttcttgg ccttaaattt tttattgaaa aaatttgctt gttaataatc aaaaaatttt
     1501 gttgtttgtt tgggctaatg ttaaaaccga ctttttgagg gtcgactaat tattaaatat
     1561 ataccaaa
//
LOCUS       DAOSMY010000345         1568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_79520_length_1568_cov_3.083939, whole genome shotgun sequence.
ACCESSION   DAOSMY010000345 DAOSMY010000000
VERSION     DAOSMY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79520_length_1568_cov_3.083939"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1568
                     /locus_tag="PLD76_06900"
     CDS             <1..>1568
                     /locus_tag="PLD76_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27763.1"
                     /translation="NCDNIQRDTVYVYAPSVIDMNLKSDYCIKEVIILAATPLGGIYS
                     SDAVANALVNNRFYTERAGLGLHKIRYIQTNEYGCISKDSTTIQVRGANELSIADLKD
                     QYCEPKGTANIYGFPNNPADGSVWFTGPTFLTSLPNGYASLDLSQATYNANYNVTYHY
                     RENYVNAQGTKESCTTTVTKGFRVLNEAADFWGYNHMDVICGTQDSVVITGNKAPLGR
                     FVFTGPAANFKDRGNGTAVIYPPTLSVGSYQVTYYYDHKNTQGVTICSTEQAKTFKIA
                     QIAPIEDISVFCSSGNNAIRIPNTELDVEYELQVNGVTFEKKMGTGSILEFSPITDNT
                     AQIKVFARLGQCVIKASQEFTVRKLTLTTTKTDITCHGANDGKMLSTISGGGYPYSYS
                     FEKISGTPMSTILDSVALNLTAGNYRFTVTDTIGCELQRQIAIIEPPLLSATVQSKSV
                     ECPTTRLGNASVTPSGGRPPYKYKWYNSAGLLIDSVASISNLTSDTYRVEVFDRSGCK
                     VERFATITTPTPIK"
BASE COUNT          517 a          279 c          306 g          466 t
ORIGIN      
        1 aaattgtgat aatatacaaa gagatactgt atatgtatat gctccttcgg taattgatat
       61 gaatttgaaa tcggattact gtatcaaaga ggtaattatt ttagcagcaa ctccattagg
      121 tggtatttat tcatctgatg cggttgcgaa tgctcttgtt aacaatcgtt tttatacaga
      181 aagagctgga ttgggtttac ataaaataag gtatattcaa accaatgagt atggatgtat
      241 ttctaaagat agtactacaa tacaagttag aggtgccaat gagttgtcga ttgcagattt
      301 aaaagaccaa tattgtgagc ctaaaggaac tgccaatatt tatggttttc caaataaccc
      361 agctgatggt agcgtttggt ttactggacc aacatttcta acaagcttgc caaacggata
      421 cgcaagttta gacttgtcac aagctacata caatgcaaat tacaatgtaa cgtaccatta
      481 tcgcgagaat tatgtaaatg cacaaggtac gaaagaatca tgtacaacta ctgtaaccaa
      541 aggctttagg gtgttaaatg aagcagccga tttttgggga tacaaccaca tggatgtaat
      601 atgtggtaca caagattctg tggtaataac aggtaataaa gccccacttg gcaggtttgt
      661 atttactgga ccagcagcca attttaagga tagaggaaac ggaacagccg tgatttaccc
      721 tccaaccttg tctgtagggt cttatcaggt tacctattat tatgaccata aaaatacgca
      781 aggtgtaact atttgctcta cggaacaagc caaaacattt aaaatagctc aaatagcacc
      841 tatcgaagat atttctgtat tttgcagtag cggtaataat gccattcgta ttccaaatac
      901 agaacttgat gtagaatatg agttacaagt aaatggggta acctttgaaa agaaaatggg
      961 aacaggatct attctagaat tttctcctat cactgataat acagcccaaa ttaaagtatt
     1021 tgctcgtctt ggacaatgtg taatcaaggc ttcacaagaa tttactgtac gcaagttaac
     1081 cctcacaaca acgaaaacag atatcacgtg ccatggagca aatgatggta aaatgctctc
     1141 aacaatatca ggtggtggct atccttattc atattcattt gaaaagattt ctggtacacc
     1201 aatgtctact atattagatt ctgtggcatt aaatcttacg gctggtaatt accgtttcac
     1261 tgttactgat accattggct gtgaattgca acgtcagatc gctatcattg aacctccatt
     1321 actatcggcc acggtgcagt caaagtctgt agaatgccct actacacgtt taggtaatgc
     1381 aagtgttact ccttctggtg gcagaccacc ctataagtat aaatggtata attcggcagg
     1441 tctgcttata gattcagtag catcaattag taatttaacc tcagacacat atcgtgtaga
     1501 agtgtttgat agaagtggat gcaaggtaga aaggtttgca actattacga ctcccactcc
     1561 gattaaaa
//
LOCUS       DAOSMY010000346         1532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_82168_length_1532_cov_2.935003, whole genome shotgun sequence.
ACCESSION   DAOSMY010000346 DAOSMY010000000
VERSION     DAOSMY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82168_length_1532_cov_2.935003"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..885)
                     /locus_tag="PLD76_06905"
     CDS             complement(490..885)
                     /locus_tag="PLD76_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOU27764.1"
                     /translation="MPVTLHHIGIWVDDLHKMRQFYEHYFSAHCSKKYINPITNFSSY
                     FLSFGNDSTRIELMCRVDTTIEPTKRGIMKGYAHIALSVGSTSKVNQLTENLRNDHYK
                     IVSEPRTTGDGYYESIVLDPEGNYIEITV"
     gene            complement(897..>1532)
                     /locus_tag="PLD76_06910"
     CDS             complement(897..>1532)
                     /locus_tag="PLD76_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU27765.1"
                     /translation="HTYQATAPNRSIFIQQNALDDKKIIALLPGSRKQELEACLPIML
                     QVDFSQFADYQFIVAATDALPCTLYHTLMKGSNAKIVYGKTYDLLQHAEAAIVTSGTA
                     TLETALFNVPQVVCYEAFLSRLVAPFLRMVLKIKYVSLVNIIAQKELVKELLIQDFTP
                     KNTQKELQKLVFEKAYRANILAGYQQLHTELGTTIASDTAAKAMVNYLKKE"
BASE COUNT          474 a          263 c          235 g          560 t
ORIGIN      
        1 aaaaaagcat cattattttt atttttaaga ctttcgattt tagtgtaaag tcttttaata
       61 tctctattta attctgaatt tgtcataata ttaaatttta attattgttt ttattacctg
      121 ctcccgaagg agcaacgctt tgagcctaaa atctatttaa agtcgcccaa acgactgtta
      181 aacgtgcata tttccaaaaa tcaaaactat tactaaaata aatgtactta tttggaaata
      241 taatgtcttc ataataaata tgttatatta atattcatgc ataaaaatac agatgataaa
      301 aataatcatt ttctattact taaacgaatg tgggcattct tctttttcta ttcttttttg
      361 tggaattttt atcggtattc gataccgttg agagcaattt cccttaggat atgcttgtat
      421 tatgttccct tttgatcgta tatttttaaa cctaattaaa aaaaaatcag acagtagtaa
      481 ggtagaatgt tagacagtaa tttcaatata attcccttct gggtcgagta cgatgctttc
      541 ataataccca tcacctgtag tacgtggttc acttacaatc ttgtaatggt cattgcgtag
      601 gttttccgtc agttgattta ctttacttgt actacccacc gatagtgcta tgtgtgcata
      661 acctttcatt atgcctcttt ttgtgggttc aatggttgta tcaaccctac acattaactc
      721 tattctagta ctatcattac caaacgacaa aaaataggaa ctgaaattcg ttatgggatt
      781 gatatatttt ttgctacaat gagcactaaa ataatgttca tagaattgac gcattttgtg
      841 caagtcatca acccaaatcc ctatatgatg gagtgtaact ggcattggct atttatttat
      901 tctttcttca aataattaac cattgctttt gctgcagtat cagaggcaat tgttgtacct
      961 aattcagtat gtaattgctg atagcctgcc aatatattag cacgatatgc tttttcgaat
     1021 actagctttt gcaattcctt ttgcgtattt tttggggtaa aatcttgtat aagtaattct
     1081 tttactaatt ctttttgggc aataatattt accaaagaca catacttaat tttcaacacc
     1141 attcgtaaaa atggagctac caaacgtgat aaaaaagcct cataacacac cacttgtggc
     1201 acattaaaaa gagctgtttc taaggttgct gtgcccgagg taacaatcgc tgcttcggca
     1261 tgttgcaata aatcataggt ttttccgtac actattttag cattgctccc tttcattaag
     1321 gtatgataca gtgtacaagg taacgcatcg gtagctgcta ctataaactg ataatcagca
     1381 aattgagaaa aatcaacttg caacattatc ggcaaacaag cctctaactc ttgcttacga
     1441 ctacctggta gcaaggcaat tattttttta tcatctaagg cattttgttg tataaaaata
     1501 ctgcgattag gtgctgttgc ttggtatgta tg
//
LOCUS       DAOSMY010000347         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_85240_length_1493_cov_2.341446, whole genome shotgun sequence.
ACCESSION   DAOSMY010000347 DAOSMY010000000
VERSION     DAOSMY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85240_length_1493_cov_2.341446"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLD76_06915"
     CDS             complement(<1..596)
                     /locus_tag="PLD76_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27766.1"
                     /translation="MTKIHKILVFISLCGLIFYSSLVANDTIDTSVSLAKKETEPSDE
                     SLRYPISQSLENAPLTQHTNAVIDLPSPQNIASSIEYDPLTEQFVFSKKLNDQDLITP
                     TYLTPEEYSKYQMQQSVNAYWRTRNRQEKDNEKPFSLTDIQIGLGDADKIFGPGGVQL
                     KLQGSAELLFGLKFYRSKNPSLSERLQSPPPVFDFDQK"
     gene            complement(617..1204)
                     /gene="ruvA"
                     /locus_tag="PLD76_06920"
     CDS             complement(617..1204)
                     /gene="ruvA"
                     /locus_tag="PLD76_06920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU27767.1"
                     /translation="MIEYIKGDVVELNPSFVVIETNGIGYFIHISLTTYAHLQQTTAC
                     QLYVYESIREDAHVLYGFSKKEERQIFLHLISVSGVGASTARVILSSLSALEIQEAIA
                     TANENVLKAVKGIGLKTAQRIIVDLKDKLGNMELATKNGVTLQVNTSMKKEAVSALVM
                     LGFPASASQKVVDKILADNADMKIEDVIKKALKML"
BASE COUNT          477 a          277 c          245 g          494 t
ORIGIN      
        1 attttttgat caaaatcaaa tacaggaggt ggactctgta atcgttcact taaagatgga
       61 tttttagacc tatagaattt aagcccgaac aataactctg ccgaaccttg aagcttcaac
      121 tgcacccctc ctggtccaaa tattttgtca gcatcgccca acccaatttg aatatcggta
      181 agagaaaatg gtttttcatt gtctttttcc tgtctattgc gtgtacgcca ataggcatta
      241 acagattgtt gcatttggta cttagagtac tcttctggcg ttagatatgt tggcgttatt
      301 aaatcttgat cgttcagttt cttagaaaaa acaaactgtt cagtcaacgg gtcatattcg
      361 atagaagatg ctatattttg tggagatggt aagtctatta cggcattcgt atgttgggtt
      421 aaaggggcat tttccaacga ttgcgaaata ggataacgta acgattcatc agatggttct
      481 gtttcttttt ttgctaaact tacagaggta tcaatagtat cgttagccac caacgaagag
      541 tagaaaatca acccacaaag gcttataaaa acgagtattt tatgtatctt agtcaaggta
      601 aacgaacgct ttattgttac aacattttta atgctttttt gataacgtct tcaattttca
      661 tatctgcgtt atctgccaaa attttatcca ccactttttg tgaagcagag gctggaaaac
      721 ctaacatcac caatgcagat accgcctctt ttttcattga ggtattaact tgcagagtaa
      781 ctccattttt agtagctaac tccatattgc ctagtttgtc tttcaaatca acaatgatac
      841 gttgtgccgt ttttaaccca attcctttta cagccttcaa cacattttca ttggctgttg
      901 caatggcttc ttgtatttct aatgccgaaa gagatgataa aataacccga gcagtactgg
      961 ctcctactcc cgacacagag ataagatgta aaaagatttg tctttcttct ttttttgaga
     1021 acccatacaa cacatgtgca tcttcgcgaa tggattcgta aacatacaat tgacaagctg
     1081 tcgtttgttg taaatgggca taggtagtaa gtgaaatatg gataaaatac cctattccat
     1141 tggtttctat tactacaaaa ctgggattta gctctacaac atctccctta atatattcaa
     1201 tcataaatac ttatctaata ttggatggct ctaaaaaagg gttcaaagat acataaaaaa
     1261 aatagtcttt tcaaaatttt ccatcacatg ctatcttata cactatataa aacgtgtgta
     1321 gctagaacga agaaaaggca aaaaacgtat ataaaataat cttttttaac gtataaaaaa
     1381 ggtagatccc aacaatgatt agtaaaaact gttatgtata tgcacactaa agctatgcat
     1441 gttacaatac ttagctacct aaccatctcg gtatccactg tatctaaatt att
//
LOCUS       DAOSMY010000348         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_85935_length_1484_cov_3.507348, whole genome shotgun sequence.
ACCESSION   DAOSMY010000348 DAOSMY010000000
VERSION     DAOSMY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85935_length_1484_cov_3.507348"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1466
                     /locus_tag="PLD76_06925"
     CDS             6..1466
                     /locus_tag="PLD76_06925"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOU27768.1"
                     /translation="MATFVVSVAVATLISFFIVKNHGLSNQAFLSSKQHAPSTFVAMS
                     EDSPIASHGFVKAAEATVNTVVHVRTKSPAKQVAFSHDPFFEFFFGYPQMERQEPRMQ
                     EGAGSGVIISSDGYIVTNSHVVASSTNIEVTLNDKRTFEATLIGSDPNTDIALIKIDA
                     KDLPVSVFGDSDALSVGEWVLAVGNPFNLTSTVTAGIVSAKARSINILSGDMKIESFI
                     QTDAAINPGNSGGALVNLQGELVGINTAIATTTGAYAGYGFAVPSSIVQKVVVDLKQY
                     GTVQRAVLGVQIADITSELAKEKGLKTLDGVYIHSVVENGAADNAGLKSEDIIQQING
                     IRVNTVAQLQEQLSRFRPGDQVTIQVLRKNKELSMRATLKNKLGNTDVVGSQVNAEEY
                     LSIRVKEIDNQTKSQLRLSYGLQITEIKKGKLFDQGIKNNYIIVSVNNQAIRTKDDLQ
                     QALDTALNAADRNKVLFITGVYPNGVRAYYAINLDE"
BASE COUNT          460 a          280 c          333 g          411 t
ORIGIN      
        1 ttgcggtggc tacttttgta gtcagtgtag cagtagcaac cctcatcagt ttttttattg
       61 tcaaaaatca tggattgagc aatcaagctt ttttatcaag caaacagcat gctccgtcca
      121 cctttgtcgc catgtcggag gatagtccca ttgcctcgca tggttttgta aaagcagccg
      181 aggctaccgt aaatacagtg gtgcacgtac gaacaaaatc gcctgccaag caagttgctt
      241 tttcgcacga cccatttttt gaattctttt ttggatatcc ccaaatggaa cgtcaagagc
      301 cacgtatgca agaaggagca ggttcgggag ttattatctc ttctgatggg tatattgtaa
      361 ccaatagcca tgtggtagca agttctacca atatagaagt tacgttgaat gataaacgta
      421 cttttgaggc gaccttaatt ggctctgacc ccaataccga tattgccctg ataaaaattg
      481 atgccaaaga tttgcctgtg tccgtatttg gcgactccga tgcacttagt gtaggtgagt
      541 gggtattggc tgtgggtaat ccgtttaacc tcacttctac cgtaacagca ggtattgtta
      601 gtgccaaggc acgcagcatc aatatcttgt cgggcgatat gaaaattgaa tcatttatcc
      661 aaacagacgc agccatcaat ccagggaata gcggaggtgc gttggtgaat ttacaagggg
      721 aattggtagg tattaatacc gctattgcta caacgactgg agcgtatgct ggttatgggt
      781 ttgctgtgcc atccagtatt gtgcaaaaag tagtcgtaga tttaaaacaa tatggcactg
      841 tgcaacgtgc tgtgttgggt gttcaaattg ctgatattac cagcgaatta gccaaagaaa
      901 aaggcttaaa aaccctagat ggtgtatata tccattcggt agtagagaat ggagcagctg
      961 ataatgcagg acttaaatcc gaagacatta ttcagcaaat taacggtata agggtaaata
     1021 cggtggctca attgcaagaa caactaagtc gttttagacc aggcgatcag gttactatac
     1081 aggtacttag aaagaataaa gaattaagta tgagggctac tcttaaaaat aaacttggca
     1141 acacagacgt agtgggttcg caagtgaatg ccgaagaata tctttcgata cgggtaaagg
     1201 aaattgataa tcaaactaaa tctcaattaa ggttgtcgta tggtttgcaa attaccgaaa
     1261 tcaaaaaagg taaattattc gaccaaggta taaaaaacaa ctacatcatt gtgtcggtaa
     1321 acaaccaagc tattcgcacc aaagacgatt tgcaacaagc cctagataca gccctaaatg
     1381 ctgccgatcg caataaggta ttatttatta ctggcgtata tcccaatggc gtaagagcgt
     1441 actatgccat taatttggac gagtaataac cagtctgaat gatt
//
LOCUS       DAOSMY010000349         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_86561_length_1477_cov_3.400844, whole genome shotgun sequence.
ACCESSION   DAOSMY010000349 DAOSMY010000000
VERSION     DAOSMY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86561_length_1477_cov_3.400844"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLD76_06930"
     CDS             complement(<1..1193)
                     /locus_tag="PLD76_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOU27769.1"
                     /translation="MSKVVIIGAGGVGTVVAHKVAQNSKVFTEVLLASRTKSKCDAIA
                     ADVQSRYGVTMQTAQVDADNVPELVALLRSFQPKLVINVALPYQDLTIMDACLEVGVN
                     YLDTANYEPKDEAHFEYSWQWAYQDKFKKAGLTAILGCGFDPGVTSVFTAYAAKHHFD
                     EMHYLDIVDCNAGDHGKAFATNFNPEINIREVSQRGKYWENGKWVETEPHEIHKPLNY
                     PEIGPKESYVIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMARIDEVE
                     YNGQKIVPIQFLKAVLPNPGDLGENYTGWTSIGCRIKGIKNGEEKTYYIYNNCSHEEA
                     YKETGTQGVSYTTGVPATVGAMMFLTGEWGGAGVFNVEEFNPDPFLHELAQQGLPLHE
                     LHNID"
     gene            complement(1213..>1477)
                     /locus_tag="PLD76_06935"
     CDS             complement(1213..>1477)
                     /locus_tag="PLD76_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU27770.1"
                     /translation="SDNQIYLSGQDIDFSISGEEYIPCQFQGEAMEIGFKASFIIELL
                     SNINASDVEFTLIDRTRPGIFTPAEQDDNKNILMLLMPMMLND"
BASE COUNT          419 a          320 c          255 g          483 t
ORIGIN      
        1 aaatcaatgt tatgcaattc gtgcaaaggc aagccttgct gtgctaattc gtgcaaaaat
       61 ggatctggat taaactcttc tacattaaat acacctgctc caccccattc gccagttaaa
      121 aacatcatag cacctactgt ggctggtaca cctgtggtat aacttacccc ttgtgtgcct
      181 gtttctttgt atgcctcttc gtggctacaa ttattataga tgtaatacgt tttttcttct
      241 ccatttttaa tacccttaat gcgacatcca atagatgtcc atcctgtata attttcgccc
      301 aaatcgcctg gattaggcaa tacagctttt aaaaattgaa ttggcacaat tttctgtcca
      361 ttgtattcca cctcatcaat gcgagccata ccaatattct gaatcacacg taagtgagtt
      421 aaatattctt gtccaaatgt catccaaaaa cgggctcttt tcagggtcgg aaaattcttt
      481 actagcgact ccaactcttc gtggtagata acgtaagatt cttttggacc aatttctgga
      541 taattcagtg gtttatgaat ttcgtgtggt tctgtttcta cccatttgcc attttcccaa
      601 tatttgcctc gttggctcac ttcacggata ttgatttcgg ggttaaaatt ggtagcaaat
      661 gctttgccat ggtcgccagc attgcaatcg acaatatcta aataatgcat ctcatcgaaa
      721 tgatgtttgg ctgcataggc agtaaatacg cttgttacac cagggtcgaa accacaacct
      781 aaaatggcag ttaaacccgc ttttttaaat ttatcttggt aagcccattg ccacgagtac
      841 tcaaaatgag cctcgtcttt gggttcgtag ttggctgtat ctaggtaatt tacacctact
      901 tccaaacacg catccatgat agttaaatct tggtatggaa gagcaacatt aatgactaat
      961 tttggctgaa aagaacgcaa caaagcgact aattcgggca cattatcggc atctacttgt
     1021 gcagtttgca tagttacacc ataacgcgat tgcacatcgg cagcaatggc atcacatttt
     1081 gatttggtgc ggctagctaa caatacctct gtaaacacct tactattttg agctactttg
     1141 tgtgctacta ccgtacctac accgcctgct cctataatta ctacttttga catattactt
     1201 ttctgttatt gtttaatcgt ttagcatcat tggcatcaac aacatcaata tatttttatt
     1261 atcatcttgt tcggctggtg taaaaatacc aggacgtgta cggtcaataa gcgtaaattc
     1321 aacatccgaa gcattgatat tgctcaataa ttcgatgata aaactggctt tgaacccaat
     1381 ctccatagct tctccttgga attgacaagg gatatattct tctcctgaaa tagagaaatc
     1441 aatatcttga cccgataaat aaatttgatt gtcagag
//
LOCUS       DAOSMY010000350         1447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_89251_length_1447_cov_2.667385, whole genome shotgun sequence.
ACCESSION   DAOSMY010000350 DAOSMY010000000
VERSION     DAOSMY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89251_length_1447_cov_2.667385"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="tsaB"
                     /locus_tag="PLD76_06940"
     CDS             <1..588
                     /gene="tsaB"
                     /locus_tag="PLD76_06940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU27771.1"
                     /translation="NHSELLGVFVQKALQEAKKQHLQLDAVAVSSGPGSYTGLRIGVS
                     MAKGLCYGFSIPLIAINSLKILAQGVVANHSIEHDAILCPMIDARRMEVYTAFFDARL
                     NILKPTWAEVLHADTFETLHDKKVYIFGNGAEKSIGIVQHPNIQFLPHQTPLASSMIV
                     LAEEAFQQQQFEDMAYFEPFYLKEFMTSIPKNKLA"
     gene            622..1233
                     /locus_tag="PLD76_06945"
     CDS             622..1233
                     /locus_tag="PLD76_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOU27772.1"
                     /translation="MEYYTEKEKLILPEYGRNIQQMVNHALTIEDKAERTRCVHSIIA
                     IMGKLFPHLRDVNDFKHKLWDHLAIMSNFTLDIDYPYEVPKQEILNTKPERIPYPSMH
                     IKYRHYGKTVDTMVKKAIEYPEGDDKKRFIEMIANYMKKSFLTWNKEGVDDEKIYEDL
                     KELSNGQLIYTSESLKLKESKEILAQNKVNKSKQNIPHKKIKK"
     gene            1238..>1447
                     /locus_tag="PLD76_06950"
     CDS             1238..>1447
                     /locus_tag="PLD76_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU27773.1"
                     /translation="MASFVIEGGHSLSGEITPQGAKNEALQVICATLLTKQKVTIHNI
                     PSILDVNNLIDLLKNIGVRVEKISKH"
BASE COUNT          533 a          259 c          273 g          382 t
ORIGIN      
        1 aatcacagtg agttactggg tgtttttgta caaaaagctc tgcaagaagc aaaaaaacaa
       61 catctgcaac tcgacgcggt tgctgtaagt agtggaccag gttcctacac ggggttacgc
      121 attggggtgt caatggctaa aggattgtgt tatggctttt ctatccctct tattgctatc
      181 aatagcttaa aaatactggc acaaggggta gtagccaatc attcgattga acacgatgca
      241 attttatgcc caatgattga tgccagacga atggaggtat atacggcttt ttttgatgca
      301 cgattaaata tactgaaacc aacatgggct gaagtgctac atgccgacac attcgaaacc
      361 ttgcacgata aaaaagtata tatttttggc aacggagccg aaaaatcaat aggcattgtg
      421 caacatccaa acatacagtt tttgccacat caaacgcctt tggcttcgag tatgattgtg
      481 cttgctgagg aggcttttca acaacaacaa tttgaagata tggcttactt tgaaccattt
      541 tacctaaaag aatttatgac ttctattcct aaaaacaaat tagcttagta aaattatatg
      601 acaacaactt acgatactgc aatggagtat tacacagaga aagagaaatt aatcttgccc
      661 gaatatggtc gtaatatcca acaaatggtc aatcacgcat taacgattga ggacaaagcc
      721 gaacgcacac gctgtgtaca tagcatcatt gccattatgg gtaaactgtt tcctcatctt
      781 cgtgatgtaa atgattttaa acacaaactt tgggatcatt tggctattat gtcgaacttt
      841 acattggata ttgactaccc atacgaagtg ccaaaacaag aaatactgaa tacaaaacca
      901 gagcgaattc cgtatcctag catgcatata aaataccgcc attacggtaa aaccgtagat
      961 actatggtaa aaaaagccat tgaatatcct gaaggcgatg acaaaaaacg ctttattgag
     1021 atgattgcca actacatgaa aaaaagtttc ttaacatgga acaaagaagg ggtagatgac
     1081 gagaaaatat acgaagattt gaaagaactc tctaatgggc aattaatcta tacgagcgaa
     1141 tcgctcaaac tcaaagaatc caaagagata ctggctcaaa acaaagtgaa taaaagcaaa
     1201 caaaacatac cgcataaaaa aattaaaaag taagtttatg gcatcttttg taatagaagg
     1261 aggacattct ttgtcgggag aaataacgcc gcaaggagct aagaatgaag cactacaagt
     1321 gatatgtgcc acattgctaa caaaacaaaa agttaccatt cataatatcc ctagtatttt
     1381 ggatgtaaac aacttaatag atttactaaa aaacattggt gtacgtgttg aaaaaataag
     1441 taagcat
//
LOCUS       DAOSMY010000351         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_90059_length_1438_cov_2.514100, whole genome shotgun sequence.
ACCESSION   DAOSMY010000351 DAOSMY010000000
VERSION     DAOSMY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90059_length_1438_cov_2.514100"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLD76_06955"
     CDS             complement(<1..446)
                     /locus_tag="PLD76_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOU27774.1"
                     /translation="MKQLTLLAISIGLLVTSCSEASNKQQKQPTPTEKKIQTTNNTLI
                     MKPIYLTKAEFLKKVVNYETNPNEWKYLGDKPAIIDFYASRCGPCKMIAPILDELAAE
                     YAGEIYIYKIDTEQEQELAAAFGIQSIPTLLLVPMNENPQIIRGALP"
     gene            complement(443..682)
                     /locus_tag="PLD76_06960"
     CDS             complement(443..682)
                     /locus_tag="PLD76_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27775.1"
                     /translation="MSKYLKNWNTVRIIRLLLGASLSIAAYFSGHYLFVGLGVWFMIQ
                     AFFNVSCCGTAGCDSETPSNSSDTVFKNEIKEYKK"
     gene            complement(675..887)
                     /locus_tag="PLD76_06965"
     CDS             complement(675..887)
                     /locus_tag="PLD76_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOU27776.1"
                     /translation="MEKITNWIKKNRLYFVGSILGGIGGYIYWYFIGCDSGGCPITSS
                     PYNSVIWGAIMGSLLLSMFKKDKKDE"
     gene            complement(1051..>1438)
                     /locus_tag="PLD76_06970"
     CDS             complement(1051..>1438)
                     /locus_tag="PLD76_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27777.1"
                     /translation="GYAVKTHDVATGKRTYTPSNAISSWINFTYGKKWIAGVFGGYHK
                     NLGFSDNILDTTANGALFGRWQNIDHIYRASTSLTYKIERWVFGAEVDYNVAAYGEVD
                     YGKKGKIKNATETSNIRGVLSATFIF"
BASE COUNT          438 a          262 c          242 g          496 t
ORIGIN      
        1 ggtaatgcac ctcgaataat ttgtgggttt tcgttcatag gtacaagcaa caaggttggt
       61 attgactgaa tgccaaatgc agctgccaat tcttgttctt gttctgtgtc tattttataa
      121 atgtaaattt ctcccgcata ttctgctgcc aattcatcaa ggatgggagc tatcatttta
      181 catggaccac accgtgatgc atagaagtcg attattgcgg gtttgtctcc caaatatttc
      241 cattcgttgg gattggtttc gtaattaacc acttttttta aaaactctgc ttttgttaaa
      301 tagattggtt tcataattaa tgtattatta gttgtttgta tttttttttc agttggtgtt
      361 ggttgttttt gttgcttgtt cgaggcttcg gaacaagatg taaccaataa gcctatggat
      421 atggcaagta aggttagttg tttcattttt tatattcttt tatttcattt ttaaaaactg
      481 tatcagaact attagaaggc gtttcggaat cgcaaccagc agtaccacaa caagatacat
      541 taaaaaatgc ttgaatcata aaccatactc ctaatcccac aaaaagataa tgacccgaaa
      601 agtacgcggc aatgctcaaa gaagctccta aaagcaatct tattatacga actgtgttcc
      661 agtttttgag gtacttactc atcttttttg tcttttttaa acatgcttaa taggagcgaa
      721 cccataatag caccccatat tacactgttg tagggcgatg aggttatagg gcatccgcct
      781 gaatcgcatc cgatgaaata ccagtagata tatccaccaa tacctcctag tatgctccca
      841 acgaagtaca gcctattttt ttttatccag tttgttattt tttccatgtg tagctttttt
      901 ttttacactg caaaaataag tattatttta caatgaaaca aatttccaat ggaaaatata
      961 tcaaaagaaa ataaaattta acaaaaaaaa taatcaaggt taaaaaaaga gtatgtaacc
     1021 ttgattatat gatggtgtgt aataaacttt ctaaaatata aaagtagctg ataaaacccc
     1081 tcttatgtta gatgtttcgg tagcattttt tatttttcct tttttgccat aatcaacctc
     1141 tccgtaggca gccacattgt aatccacctc agccccaaaa acccaacgtt ctattttata
     1201 tgtaagcgaa gtagatgcac ggtaaatatg gtcaatgttt tgccagcgac caaacaaagc
     1261 accattggca gttgtatcaa gaatgttatc agagaaccct aaatttttgt ggtaccctcc
     1321 aaaaacacct gctatccatt ttttaccata cgtaaagttt atccatgacg aaattgcgtt
     1381 tgatggtgta taggtacgtt tgccagttgc tacatcgtgt gtttttacag catatccg
//
LOCUS       DAOSMY010000352         1434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_90401_length_1434_cov_3.190718, whole genome shotgun sequence.
ACCESSION   DAOSMY010000352 DAOSMY010000000
VERSION     DAOSMY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90401_length_1434_cov_3.190718"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>1434)
                     /gene="asnB"
                     /locus_tag="PLD76_06975"
     CDS             complement(131..>1434)
                     /gene="asnB"
                     /locus_tag="PLD76_06975"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HOU27778.1"
                     /translation="AFALYDIEKDEYLIARDHIGIIPLYMGWDKNNQFYVASELKALE
                     GTCVRIEEFLPGHYLSSKDCKITPWYKRDWMKYDAVNNNSSSIDELREALEKAVERQL
                     MTDVPYGVLLSGGLDSSIISAVAKKYSTKRIESGSIQDAWWPQLHSFAVGLDGSPDLI
                     AARKVADYIGSVHHEIHFAIEEGLDAVSDVIYHLETYDVTTVRASTPMYLMARVIKSM
                     GVKMVLSGEGADEIFGGYLYFHKAPNAKAFHEETVRKLSKLHSYDCLRANKSLAAWGV
                     EGRVPFLDKEFMDVAMRLNPTDKMAGNGKMEKWILRKAFESYLPEEVVWRQKEQFSDG
                     VGYSWIDNLKLIANERITDTMMKNATHTFPINTPETKEAYLYRTLFTEHFPSRTAAET
                     VPYERSVACSTAIALEWDAEFKKMADPSGRAVKSVHTDAYK"
BASE COUNT          435 a          293 c          247 g          459 t
ORIGIN      
        1 acaaagatag ggtgtatggc acagataaag tgtaacattt gttacactta caaaataaat
       61 ggttgtgcct agatttagac acaaccattt agaaaataaa aactataaaa atgaaaaact
      121 aacagatact ttatttgtag gcatcagtat gaactgattt tacagctctt cctgatgggt
      181 ctgccatttt cttaaattca gcatcccact ccaatgctat agcggtacta caggctacag
      241 accgttcgta tggaacagtt tcggctgcag tacggcttgg aaaatgttct gtaaatagtg
      301 tgcgatataa atatgcctct tttgtttcgg gtgtattaat tggaaaagta tgagtagcat
      361 ttttcatcat ggtatcggtt atgcgttcat tggcaatcag ttttaagttg tctatccagc
      421 tatatcctac accatcagaa aattgttctt tttgacgcca cacaacctct tcgggtaaat
      481 acgattcaaa ggctttacgc aatatccatt tttccatttt tccattacca gccattttat
      541 ctgtaggatt taagcgcata gcgacatcca taaattcttt gtctaaaaat ggaacgcgac
      601 cttctacacc ccaagctgct aaggatttat tcgctctcaa acagtcgtaa gaatgcagtt
      661 tggataattt tcgcactgtt tcttcatgaa aagctttagc gtttggagct ttatggaaat
      721 acaaatatcc gccaaaaatt tcgtcagcac cctcgccaga taataccatt tttactccca
      781 ttgatttgat tacacgtgcc atcagataca taggggtaga agccctaacc gtggttacat
      841 catatgtttc gagatgataa ataacgtcac ttacagcatc taaaccttct tcgatggcaa
      901 agtgtatttc gtggtgtact gaaccaatgt agtcagctac tttacgagca gcaataaggt
      961 cgggcgaacc atctaatcct acggcaaatg aatgtagttg aggccaccaa gcatcttgaa
     1021 tactgccaga ctcaatacgt ttggttgaat atttttttgc tactgctgaa ataatagatg
     1081 aatctaatcc tccagacaat aatacgccat atggtacatc tgtcattaat tgtctttcaa
     1141 ctgctttttc tagagcctct cgcaattcat caatggaaga agagttgttg tttaccgcat
     1201 catatttcat ccaatcccgt ttgtaccatg gcgtgatttt gcaatcttta ctacttaaat
     1261 agtgcccagg taaaaattcc tcaatacgca cacaggttcc ttctaaggct ttaagttcag
     1321 atgcaacata aaattgattg tttttatccc aacccatgta caatggtata attccaatat
     1381 gatcgcgtgc aattaaatat tcatctttct caatatcata tagtgcaaat gcaa
//
LOCUS       DAOSMY010000353         1421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_91609_length_1421_cov_3.159590, whole genome shotgun sequence.
ACCESSION   DAOSMY010000353 DAOSMY010000000
VERSION     DAOSMY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91609_length_1421_cov_3.159590"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLD76_06980"
     CDS             complement(<1..368)
                     /locus_tag="PLD76_06980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27779.1"
                     /translation="MKLSIVIVNYKVPHLLLQCLDAVEKAIQCMDAEVIVVDNNSNDQ
                     SRDLVSTYFSSVIYIANAENVGFSKANNQAIKIAKGEYVLLLNPDTIIAEDTLKTVCD
                     IADTTDRFGALGVRMVDMKGA"
     gene            complement(402..824)
                     /locus_tag="PLD76_06985"
     CDS             complement(402..824)
                     /locus_tag="PLD76_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27780.1"
                     /translation="MIIAVDFDGTIVEHAYPSIGDEIPFAIETLKKIQDEGEHQLVLW
                     TVREGELLQEAVEYCRKKGLEFYAVNKDYPEEEYDIRANRKLKADLFIDDRNVGGLPD
                     WGVIYAMIKGKVKPKYDGGYEIPKRKNVFIHIGEWLQK"
     gene            complement(862..1302)
                     /locus_tag="PLD76_06990"
     CDS             complement(862..1302)
                     /locus_tag="PLD76_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27781.1"
                     /translation="MEKEIKTIQKTLLKFYYLLYATLMLILIAGGFLLPKFDPLQAVT
                     VQILNTISIALVLVCIPLGLKYFSDKTKKLKNEVTDKQTRIVSYVRYAKQLMFVLGLP
                     GIFAGVSYYLIPEDKTSLFCFLMSFVALVLSKPTEVKLQKFFIE"
     gene            complement(1317..>1421)
                     /locus_tag="PLD76_06995"
     CDS             complement(1317..>1421)
                     /locus_tag="PLD76_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HOU27782.1"
                     /translation="ARGISIGDELEYADEVTLGRSIVHRVPFASSLQL"
BASE COUNT          452 a          292 c          200 g          477 t
ORIGIN      
        1 gcccctttca tatctaccat tcgtacacct aatgctccga aacgatcggt agtatccgct
       61 atatcgcata cagttttcaa tgtatcctca gctataatag tatctggatt gagcaaaaga
      121 acatactctc cttttgctat ttttatcgcc tgattgtttg ctttggaaaa accaacattt
      181 tcggcattag ctatgtaaat aacagaagag aaatatgtag agactaaatc gcgtgattgg
      241 tcattggaat tattgtccac cacaattacc tcagcatcca tacattgaat tgctttttct
      301 acagcatcta aacattgcaa cagcaaatgg ggcaccttat aattaacaat gacaatagac
      361 aacttcatac attaaaaata aatatcaaca actcactact tctatttctg taaccactct
      421 ccgatatgga taaatacgtt ttttcgtttt ggaatttcat aaccaccatc gtatttaggt
      481 tttactttcc ctttaatcat ggcatatatt accccccaat caggtagtcc tcccacattt
      541 ctatcatcaa taaataaatc agcctttagc ttacgattgg ctcttatgtc gtattcttct
      601 tctggataat ctttattaac ggcataaaat tctaagcctt tttttcgaca atattccaca
      661 gcctcttgca ataattctcc ttctcgtact gtccaaagca ccaactggtg ctctccttcg
      721 tcttgaattt tttttagggt ttcgatggca aatggtatct catcaccaat gcttggatag
      781 gcatgctcaa caatagttcc atcaaaatca acagctatta tcataggtat ttgttatata
      841 tactaggtac caaacttata tttattctat aaaaaacttt tgcaatttaa cttcagtagg
      901 tttagaaagg actaatgcta caaacgacat taaaaaacaa aacaaggaag ttttatcttc
      961 tggtatcaga taatacgata caccagcaaa tatgcctggc aaacctagca caaacataag
     1021 ctgtttagca tagcgaacat agctcacaat acgtgtttgc ttatcagtta cttcgttttt
     1081 taactttttt gttttatccg aaaaatactt taatcctaag ggtatacata ccaacaccaa
     1141 ggcaatagaa attgtgttta gtatttgtac tgtaacagcc tgtaatgggt caaacttggg
     1201 tagcaaaaat ccaccagcta tcaatataag catcaatgtg gcatacagta gataataaaa
     1261 ctttagcaac gttttctgta ttgtttttat ttctttttcc atatattgta ttaaaattat
     1321 agttgtaatg aagatgcaaa tggaacacga tgcacaatag atctgcccaa tgttacttca
     1381 tcggcatatt ccaattcgtc tcctattgaa ataccacgtg c
//
LOCUS       DAOSMY010000354         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_91817_length_1419_cov_2.777859, whole genome shotgun sequence.
ACCESSION   DAOSMY010000354 DAOSMY010000000
VERSION     DAOSMY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91817_length_1419_cov_2.777859"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLD76_07000"
     CDS             <1..829
                     /locus_tag="PLD76_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU27783.1"
                     /translation="VAMIVGQGALVMLAGYLETTMPSIAIAWSITFYGIAVFFFALVL
                     YHWFVLPKPSTDIVRNNKRVVTDFFATFGAFFTKKGIWLSIVFILLYRLGEAQLVKIA
                     SPFLLDPRSVGGLGLTTSEVGFVYGTIGVIALTLGGILGGIVAAKDGLKHWLWIMVIA
                     MNLPNLVYVYFAYMQPDNFYIIASGVAIEQFGYGFGFTAFMLYLIYIAKGEFQTAHYA
                     IATGFMALGMMLPGMFAGILQEYMGYFPFFVWVCICTIPGFLLIPFLKIDPSFGKKE"
     gene            836..>1419
                     /locus_tag="PLD76_07005"
     CDS             836..>1419
                     /locus_tag="PLD76_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOU27784.1"
                     /translation="MNTTKYKMLVLDLDDTLLRDDYSISERTINALIATQQMGVKVVL
                     ASGRPTLAMTQYAKMLQQHNFDSYMISFNGAVVSSTRTNEVLFETSLTQSEIHSLYDF
                     SVEHGVHIITYSDKGIVSETHSEYIDVELKLTGIPHYKVPSFKDEVSYSAIKCILLEH
                     PDYLKQVELVLKSQRTDLSVSRSKPFFLEVMPKG"
BASE COUNT          408 a          220 c          297 g          494 t
ORIGIN      
        1 tgttgcgatg attgtggggc aaggagcctt ggtaatgctt gctggctatt tagaaacaac
       61 catgccctct atcgccattg catggagtat tacattttat ggaatagctg tttttttctt
      121 tgcattggtt ctttatcatt ggtttgtatt accaaaacca tcaacggata tcgttagaaa
      181 taataaacgt gtagttacag attttttcgc tacatttggt gcttttttca ctaaaaaagg
      241 catttggttg tccattgtgt ttatattgct ctatcgcttg ggagaagctc aattggttaa
      301 aattgcatcc ccatttttac ttgacccacg tagtgttggg ggcttaggat taactacctc
      361 tgaagtgggt tttgtatatg gtaccatagg tgtaattgca cttacgttag ggggtatttt
      421 gggaggtatt gtggcagcta aagatggatt aaaacattgg ttgtggataa tggttatagc
      481 catgaactta cccaacctag tgtatgtgta ttttgcttat atgcaacctg acaattttta
      541 tatcatagct tcaggtgtgg ctatagaaca atttggctat ggatttggtt tcactgcctt
      601 tatgctgtac cttatatata ttgccaaagg agaatttcaa acggcacatt atgctatagc
      661 tactggtttt atggctttag gaatgatgtt gccgggcatg tttgcaggaa ttttgcaaga
      721 atatatgggt tatttcccat tttttgtatg ggtgtgtatt tgtaccattc ctgggttttt
      781 attaatacca tttttgaaaa ttgatccatc atttgggaag aaagaataac aagaaatgaa
      841 cacaactaag tataaaatgt tggtgctcga cttagatgat accttacttc gagacgatta
      901 tagtatttca gagagaacca ttaacgctct tattgctacc caacagatgg gggtaaaggt
      961 tgtattagca tctggcagac caactttagc tatgactcaa tatgctaaga tgttgcaaca
     1021 acacaatttc gactcttata tgatttcgtt taatggagca gtagttagtt ctaccagaac
     1081 gaatgaagtt ctttttgaaa caagtttaac ccaatcggaa atccacagtt tatacgattt
     1141 tagcgtagaa catggtgtac atattattac gtattcagac aagggtattg tgagcgaaac
     1201 tcactctgag tatattgatg tagaactaaa actgacagga atacctcatt acaaagtacc
     1261 tagttttaaa gatgaagtta gctattcggc tataaaatgt attttgttag agcatccaga
     1321 ttatttaaaa caagtggaat tggtacttaa atcgcaacgt acagatttga gtgtgtctcg
     1381 ttctaagccg ttttttttag aagtgatgcc caaaggaat
//
LOCUS       DAOSMY010000355         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_92128_length_1416_cov_3.380602, whole genome shotgun sequence.
ACCESSION   DAOSMY010000355 DAOSMY010000000
VERSION     DAOSMY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92128_length_1416_cov_3.380602"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLD76_07010"
     CDS             complement(<1..288)
                     /locus_tag="PLD76_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27785.1"
                     /translation="MKKILLSLVALFATVSLVSAITYTVTVPAGTNTVYIAGSMNAWN
                     ASANEMTKVDATTFTLTIADATELDEYKYCSGPGWIYVEKTESGTEVANRTW"
     gene            complement(372..1370)
                     /locus_tag="PLD76_07015"
     CDS             complement(372..1370)
                     /locus_tag="PLD76_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27786.1"
                     /translation="MKKVLIILLSLIAMVWSMNAAPGELTTIPSNPTVNNSITLIYEP
                     LPNQSWMLSQDVFIYTCLEFDENGEWVKQKAEWSKTSLPNFKWVKEDDGKLYYIISNI
                     KTFFNLTDDEVSRVSGIFVILKNEKFQSTDKYVAIRGERKPVEKFTGTVSFEVKVPEG
                     TKSVYVAGTFGPEGTPNFWKHADEKYKLTRKDATTFVGVIKDVPEDLQYLYVYGSRVD
                     QAEFRMGHRPLGGKTKVHDVVEYWGDMTLNVSVPKGTKEVYVSGNFNNWGHSLMTDIG
                     NNTWIYHVAPVKIGADEQVEYKYYTKADKTASEPQQNNRKLKFVGFSTQYDEVTKW"
BASE COUNT          437 a          289 c          189 g          501 t
ORIGIN      
        1 ccatgtgcga ttagcaacct ctgttcctga ttcagttttt tcaacatata tccatcctgg
       61 accactgcaa tatttgtact catctaattc tgtcgcatcg gcaatagtta aagtaaaagt
      121 tgtagcatct acctttgtca tttcattagc tgaagcattc caagcattca ttgaacctgc
      181 aatataaaca gtattcgtac cagcaggtac agtaacagtg taagtaatgg cacttaccaa
      241 actaacagta gcaaatagag ctactaaaga aagtaaaatc tttttcattg ttgtaaaatt
      301 tttaattaat aaatagttaa agaataaaag aataaatata attaggtttt aaattatatg
      361 tacattaaca cttaccattt ggtaacctca tcgtattgtg tagagaaccc tacaaatttc
      421 aatttacggt tattttgttg tggctcgctt gccgttttat ctgctttggt ataatatttg
      481 tattccacct gctcgtcagc acctattttt acaggagcta catgataaat ccatgtatta
      541 ttacctatat cagtcatcaa actatgtccc cagttattaa aattaccaga aacatacact
      601 tctttagttc cttttggcac agacacattc aatgtcatat ctccccaata ctcaaccaca
      661 tcgtgtactt ttgttttgcc tcctaatggg cggtgtccca tacgaaattc agcttgatct
      721 actcttgaac catacacata caaatattgt aaatcctctg gcacatcctt gataacaccg
      781 acaaatgtag tagcatcttt tcttgttaac ttatattttt catcagcatg tttccaaaag
      841 ttgggtgttc cttctggtcc aaatgttcca gccacgtata cactttttgt tccttcaggt
      901 acttttacct caaaagaaac agttccagta aatttttcta caggttttct ttctcctctt
      961 atggctacat acttgtctgt agattgaaat ttctcatttt tcaaaatcac aaaaatacca
     1021 gaaactctag aaacttcatc atccgttaaa ttaaaaaatg ttttgatatt tgatataata
     1081 taatacaatt ttccatcatc ctcttttacc catttaaaat taggcaaact cgttttactc
     1141 cattcagctt tttgttttac ccactcccca ttctcatcaa actctaaaca cgtataaata
     1201 aaaacatctt gtgatagcat ccaactttga tttggaagag gctcgtagat aagagtgatt
     1261 gagttattta ctgtcggatt cgacggtatc gtcgttaatt cgccaggtgc agcattcatg
     1321 ctccaaacca tggctatcaa gcttaaaaga ataattaaaa ctttcttcat atcattaatt
     1381 ttttaaggtg tttttctttt caacaaaaag acgttt
//
LOCUS       DAOSMY010000356         1400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_93623_length_1400_cov_3.314498, whole genome shotgun sequence.
ACCESSION   DAOSMY010000356 DAOSMY010000000
VERSION     DAOSMY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93623_length_1400_cov_3.314498"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1393
                     /locus_tag="PLD76_07020"
     CDS             248..1393
                     /locus_tag="PLD76_07020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU27787.1"
                     /translation="MQNFIFQNTTKLIFGKGSIASLANAIPSTARLLLVFGGGSVRTN
                     GVYDQVIASLSLHTTTEFWGIEPNPTVETIRKAVLVGKDCRADYVLAIGGGSVLDAVK
                     LIACAIPSNDDAWQLVLNGKLIKNPLPFASVMTLPATGSEMNRGSVISNKATQEKFAF
                     YAQYPQFSILDPETTFSLPKVQLANGIADTFVHVMEQYLTTPNQSLLMDRWAEGILQV
                     LVEIAPSVLANNRDYDVMSQFMLAATMALNGFISMGVSQDWATHMIGHELTALHGIDH
                     AQSLATVLPGTMKVLQTQKAEKILQYGARVWGILQGDDNQRITKSIEATETFFRSLGL
                     ATRLSQLQIGDATIMEIEKRFMQRNVHFGEARTVTAQVTTAILNEVR"
BASE COUNT          397 a          277 c          311 g          415 t
ORIGIN      
        1 cgcacgaact acgctaaacg ctaaacgcta aacgctaaac caacgctact gcaagattaa
       61 tgtcattacc cgcacgattg tattgcttag gcaggaggta ggtatatata catagttgat
      121 agtttttgac gaataacgaa aaacgttgtt cgttttctct ctatacacaa aacattcaac
      181 gccaaccatt cactactttc aacttatttt tttatctttg taaggtatca attcatactt
      241 tatatatatg caaaacttta tatttcagaa taccacaaaa ttaatttttg gcaaaggtag
      301 tattgcttcg ttagcaaatg caattccttc tacagcacgt ttgttgttag tatttggagg
      361 tggtagtgta cgtacaaatg gtgtgtacga tcaggtaatc gctagtttat cactgcacac
      421 tactaccgaa ttttggggta ttgaacccaa tccaacagta gaaaccattc gcaaggctgt
      481 gctagttggc aaggattgta gagccgacta tgtgttggca ataggtggtg gttctgtgtt
      541 agatgcggtt aaattgattg cttgtgccat tccgagcaac gatgatgctt ggcagctggt
      601 gcttaacggt aagctgataa aaaatccctt gccctttgct tcggttatga ccttacctgc
      661 tacgggttcg gaaatgaacc gtggttcagt tatttccaat aaggctacgc aagagaaatt
      721 tgccttttat gcacagtatc ctcaattttc tattctcgac ccagaaacga ccttttcctt
      781 acccaaggta caattggcta atggcatagc ggataccttt gtgcatgtga tggagcagta
      841 tcttaccacc cccaaccaat cgcttttgat ggatagatgg gcagagggga tattgcaggt
      901 attggtagag atagctccta gtgtgcttgc caacaaccgc gattacgatg tgatgagcca
      961 gtttatgtta gctgctacta tggctctcaa tggttttata agcatgggtg tttcgcaaga
     1021 ctgggctaca cacatgatag ggcacgagct tacagctttg cacggtatcg accatgcaca
     1081 gagtttggct accgtattgc caggtacgat gaaagtattg caaacgcaaa aagctgaaaa
     1141 aattttgcaa tacggtgctc gggtatgggg tattttgcaa ggagatgata accagcgaat
     1201 tactaaatcc atagaagcta ccgaaacgtt tttccgctca ttgggattag ccactcgcct
     1261 tagtcaactc caaattggtg atgctacgat aatggaaatt gaaaaacgat ttatgcagcg
     1321 taatgtgcat tttggcgaag cacgtaccgt tacagctcaa gttactacag ctattttgaa
     1381 tgaagtgagg taggggcaga
//
LOCUS       DAOSMY010000357         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_95444_length_1383_cov_2.170181, whole genome shotgun sequence.
ACCESSION   DAOSMY010000357 DAOSMY010000000
VERSION     DAOSMY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95444_length_1383_cov_2.170181"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1383)
                     /locus_tag="PLD76_07025"
     CDS             complement(<1..>1383)
                     /locus_tag="PLD76_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU27788.1"
                     /translation="YDFSYSHNDKTWVDYEAFNQALKNNPTRSVFYDPSVGADPLNPN
                     YADYYNYGGYVETDGFSSYNPVSIIRGIDDDGRADVFLGSVRATLSLTNKLRLGTFVS
                     YQTGNEWTGKYWDKTTKYNEGYSRNGIASHSYKNYQNQLIENTLQYTDQIAAHSISGL
                     LGQSYQYNVWSGFSATNTDFITNLYSYNNLGAGMGVKNNDDRIGMGSYQDSDKLASFF
                     ARAMYNYNQKYYVNASIRMEGSSRFGPQAHPVLGRYGLFPAISGSWRMKEESFLRSVK
                     DINDLKIRAGVGVTGNIPGTTNLYFEKVGPTGNYLWLDGEFVQPWGITSNVNENLRWE
                     KKTEYNLGIDGEFFNSKLSATLDGYYRNTTDLLWEYEMPQPPYPFGTMWDNHGQIENY
                     GVEMAVNGNVFKNSDWKIDAGIVAAWNRNMVTRITSGYTKGKEGANLPYLNVGYLSGD
                     GLTGVNIMRL"
BASE COUNT          431 a          302 c          196 g          454 t
ORIGIN      
        1 caagtcgcat tatatttact ccagtaagcc catctccact cagatatcca acatttaagt
       61 acggcaaatt agcaccttct tttccttttg tatatccaga agtaatccta gtaaccatat
      121 ttctattcca agctgcaaca attccagcat ctattttcca atcactattt ttaaatacat
      181 taccatttac tgccatttct accccgtagt tttctatttg tccatgatta tcccacattg
      241 ttccaaatgg ataaggaggt tgtggcattt catattccca caacaaatca gttgtgttac
      301 ggtaataacc atctaaagta gcagacaatt tactattaaa aaactcccca tctataccaa
      361 ggttgtactc cgttttcttt tcccaacgca agttttcgtt tacattagaa gtaatacccc
      421 atggttgaac aaattctcca tctaaccata aataattccc tgttggaccc accttctcaa
      481 aatacaaatt tgtagttcca gggatatttc cagttacccc taccccagca cgaattttta
      541 aatcgttaat atcttttaca gaacgtaaga aactttcttc tttcattctc cagctaccac
      601 tgatagcagg aaacaaacca taacgcccta atacgggatg tgcttggggt ccaaaacgag
      661 aagatccttc catacgaata cttgcattta catagtattt ttggttgtaa ttgtacatag
      721 cacgtgcaaa aaaggaggct aacttatcag aatcttggta acttcccata ccaatgcggt
      781 catcattatt ttttacgccc atacctgcac ctaaattgtt atacgaatac aagtttgtta
      841 taaaatctgt atttgttgca ctaaagccac tccatacatt gtattggtag gattgaccta
      901 acaaaccaga aatactgtga gcggctattt ggtcggtata ctgtaaggta ttctcaatta
      961 attgattttg ataattctta tagctatgtg aagctattcc attacgagaa taaccttcat
     1021 tatacttagt tgttttgtcc caatacttac cagtccattc atttccagtt tgataagaaa
     1081 caaaggtacc tagacgcaat ttatttgtta aactcaaggt agcacgtaca cttcctaaaa
     1141 acacatcggc acgtccatca tcgtctatcc ccctaataat ggagactgga ttataagaag
     1201 agaatccatc tgtttctaca tatcctccat aattataata atctgcataa ttgggattca
     1261 aaggatcagc acctacagat ggatcataaa atacagaacg tgtaggatta ttttttaatg
     1321 cttggttaaa tgcctcgtaa tctacccatg ttttatcatt atggctataa ctaaagtcgt
     1381 atg
//
LOCUS       DAOSMY010000358         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_95650_length_1381_cov_2.207391, whole genome shotgun sequence.
ACCESSION   DAOSMY010000358 DAOSMY010000000
VERSION     DAOSMY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95650_length_1381_cov_2.207391"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLD76_07030"
     CDS             complement(<1..1311)
                     /locus_tag="PLD76_07030"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU27789.1"
                     /translation="MVKRIILSIAFITFIQFANANEGMWILPSIPDSIAKNMDELGYE
                     LKMRDLYNEKKSSLKDVVVSFSNGYTGVMISSDGMLLTNYTAIMPFIEQLDESSQYKE
                     EGFWASSYTREIPLHHLSVSIVKTTENVTNRILANIPEFETQEKKQHLIDSISQLLIK
                     EKQATTSYIVDFKASNKGTQFHLYTYEKFNDIRLVYAPPTSIALFGEEKDKRSTPKHN
                     ANFAVLRVYVSQENQPTPYDPYNKPYNTKQYAKISTRGYEMDDFVVTVGYPSFTNRQI
                     TSFALKEQVYAINQAELNVALVKDKVWEHAMRESNYVKNLYEKKYTQNNERLHYLIQL
                     QESVKKQNIIAEKERTEKNFMQWSIKSDFNTMLKYEHVVPTLENKYTSRKQEMYHVHF
                     LTESFVKLDILTVAYMLLTISPQNEREIYSEIAKFYERYDEQTEK"
BASE COUNT          383 a          211 c          238 g          549 t
ORIGIN      
        1 tttctctgtt tgttcatcat aacgttcata gaactttgct atttcgctgt atatttccct
       61 ttcgttttga ggagaaatcg tcaataacat ataggctact gtaagtatat caagcttaac
      121 aaaagattct gttaagaaat ggacatggta catttcttgt ttgcggcttg tatatttatt
      181 ttctaacgta ggtactacgt gttcatattt aagcatggtg ttaaaatcgg attttatgga
      241 ccactgcata aaattctttt ctgtacgttc tttttcagct atgatgtttt gtttttttac
      301 tgattcttgt aattggatta aataatgtag tcgttcgttg ttttgagtat attttttttc
      361 gtacaagttt tttacataat tactttctct catagcatgt tcccatactt tatctttcac
      421 tagggcaaca ttcagttcgg cttgattgat agcatacacc tgttctttca gtgcaaatga
      481 tgtaatctgt ctgttggtaa aacttggata tcctaccgtt acaacaaaat catccatctc
      541 gtatccacgt gtgcttattt ttgcatattg tttggtattg taaggcttgt tatatgggtc
      601 gtatggggtt ggttgatttt cttgcgaaac atacactctg agtactgcaa aatttgcatt
      661 gtgttttggg gtagagcgtt tatctttttc ctccccaaat agtgcaatgg aggtaggagg
      721 ggcatatacc aaacgaatat cattaaattt ttcgtatgtg tataaatgga attgggtacc
      781 tttattactt gctttaaaat caacaatata tgaggttgta gcttgttttt cttttattaa
      841 tagttgactt atagaatcta tcaagtgttg ttttttttct tgagtttcaa attctggaat
      901 gttggcaaga attctattgg taacgttttc tgttgttttt actatgctaa cagatagatg
      961 atgcaatgga atttcgcgtg tataagagct tgcccaaaaa ccttcttctt tgtattgaga
     1021 ggattcatct aactgctcaa taaatggcat aatcgctgtg taattagtaa gcaacattcc
     1081 gtccgaagaa atcattacgc ctgtgtatcc atttgaaaat gaaacgacta catcttttaa
     1141 agaagatttt ttttcattat ataaatcacg catttttaat tcatatccca gttcatccat
     1201 gttcttagca atggagtctg gtatggatgg taatatccac ataccttcgt tggcattggc
     1261 aaactgtatg aatgtaataa aagctatact taagattatt ctttttacca tattattttt
     1321 attgctataa ggttgctaat tacctgcaaa tatacaaaat taatttagta tagataaaat
     1381 a
//
LOCUS       DAOSMY010000359         1373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_96462_length_1373_cov_2.862671, whole genome shotgun sequence.
ACCESSION   DAOSMY010000359 DAOSMY010000000
VERSION     DAOSMY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96462_length_1373_cov_2.862671"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLD76_07035"
     CDS             <1..581
                     /locus_tag="PLD76_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU27790.1"
                     /translation="QQAIVNDISTISAVLLTHEHFDHVTGIDDLRAYGEVEIYAEERV
                     CQSLKRTIPYCFGEIKYPGVPLISLHEIDEMPFDLHNTEIVPIRIWHHKLPILGFRIG
                     AFAYLTDISGIDDKEYEKLQNLDVLVVDALRLKPHFSHFTLEQAISFAEKTNAKEVYF
                     THISHQLGKHDEIQSLLPHNMMLAYDGLKITI"
     gene            716..1027
                     /gene="rplU"
                     /locus_tag="PLD76_07040"
     CDS             716..1027
                     /gene="rplU"
                     /locus_tag="PLD76_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU27791.1"
                     /translation="MYAIVEIAGQQFKVEKDRTLFVHRLNEERGATVEFDKVLLIDAD
                     GKVKVGAPTIKGAKVLAEVISHVKGEKVIIFKKKRRKGYRVRNGHRQCFTEIMIKELV
                     A"
     gene            1047..1310
                     /gene="rpmA"
                     /locus_tag="PLD76_07045"
     CDS             1047..1310
                     /gene="rpmA"
                     /locus_tag="PLD76_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU27792.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIFGGQYAKAGSIIIRQRGTV
                     HHPGENVGIGKDHTLFALIEGTVVFRRKKDNRSFVSVQAIEQN"
BASE COUNT          502 a          203 c          264 g          404 t
ORIGIN      
        1 gtcaacaagc cattgttaat gatatttcca caatatcagc tgttctgctt acacacgagc
       61 attttgacca cgttactggc atagatgatt tacgagccta tggcgaagta gaaatttatg
      121 cagaagagcg agtgtgtcaa tcattaaaac gaaccatacc gtattgtttt ggcgaaatta
      181 aatacccagg agttcctctt atttcgttac acgaaattga tgaaatgcct tttgatttac
      241 acaatacgga gattgttccg atacgtattt ggcatcataa attacctatt ttaggtttta
      301 gaataggtgc ttttgcttat ttaaccgaca ttagtgggat agatgacaaa gaatacgaaa
      361 aactccaaaa tttggatgta ttagtagtag atgcactacg ccttaaaccg catttttctc
      421 actttacctt agaacaagcc atcagttttg cagaaaaaac aaatgctaaa gaagtgtatt
      481 ttacacacat tagccatcag ttgggcaaac atgatgagat tcaaagttta cttcctcaca
      541 atatgatgtt ggcatacgat gggttgaaaa tcactatatg acacaaaaaa gaatataaat
      601 tacttgtaca gaataaaatt atatagtatc tttgccactc aaattctgaa aggtagaaaa
      661 gaaatgcagc taaagcattc acctcacgaa ggtattttat aattaattta taactatgta
      721 cgctattgta gaaattgcgg gacaacaatt taaagttgaa aaagacagaa cattgtttgt
      781 tcatcgctta aacgaagaaa gaggtgcaac tgtagaattt gacaaagttc tattgattga
      841 tgctgatgga aaagtaaaag ttggtgctcc aactataaaa ggagcaaaag tattggcaga
      901 ggttatttct catgtaaaag gagaaaaagt aatcattttc aaaaagaaaa gaagaaaagg
      961 ttatagagta agaaacggtc atcgccaatg ttttacagag attatgatta aagaactagt
     1021 tgcttaacaa ataaaaattt acagaaatgg cacataagaa aggagtcggt agttcaaaga
     1081 atggacgtga atcggaaagt aaacgacttg gagtgaaaat atttggaggt caatatgcta
     1141 aggcaggtag tatcattatc agacaacgtg gtacagttca tcacccaggt gagaatgtag
     1201 gtatcggtaa agatcataca ttatttgctt taatcgaagg tactgttgtt tttagaagaa
     1261 aaaaagacaa tcgctctttt gtttctgttc aagcaatcga acaaaactaa gtttagaaaa
     1321 ctatatttat aaaaaccctc ctggattctt cttgaagaaa agggtgggtt ttt
//
LOCUS       DAOSMY010000360         1372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_96547_length_1372_cov_3.606682, whole genome shotgun sequence.
ACCESSION   DAOSMY010000360 DAOSMY010000000
VERSION     DAOSMY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96547_length_1372_cov_3.606682"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLD76_07050"
     CDS             <1..859
                     /locus_tag="PLD76_07050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU27793.1"
                     /translation="NQVMASVALRAKTDKIILGGMGLTNDDYLYFKNVFENAGALEKI
                     ISFVNTTDNPPVERLLIPDMALTAAEYFAVDKNESVLVLLTDMTLFADALSIVSNRMD
                     QIPSKDSMPGSLYSDLARIYEKAVQFPSGGSITIIAVTTLSGGDITHAIPDNTGYITE
                     GQLFLRRDTEIGKVIVDPFRSLSRLKQLVIGKQTREDHPQVMNAAIRLYADASNARTK
                     MENGFDLTDYDERTLEYAKAYSEKLLAIDVNVDTETMLSISWDLFSTHFSKAEVGIKA
                     AITEKYWKN"
     gene            875..>1372
                     /locus_tag="PLD76_07055"
     CDS             875..>1372
                     /locus_tag="PLD76_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU27794.1"
                     /translation="MAIKFQYNKTSLQHLDKQLKVRKRALPTLKNKESALRSEVKRAK
                     DKAATLEEEFDKEWNKYTYMVKLWGEFDQSLIRVEDVVLSSKKIAGVLTPLLDEVKFS
                     IAPYNPFTAPMWFADGIEILRSLATIGINRAVWLRKMELLEFARKKTTQKVNLYEKVQ
                     IPGFEE"
BASE COUNT          479 a          260 c          267 g          366 t
ORIGIN      
        1 caatcaagta atggctagcg tagcgttgcg tgccaaaacc gataagatta ttttaggagg
       61 tatgggactt actaacgatg actatttgta ttttaaaaac gtatttgaaa atgcaggagc
      121 attagaaaaa atcatcagct ttgtaaatac taccgacaac cctcctgtag aacgcttatt
      181 aataccagat atggctctta ctgcagccga gtatttcgca gttgataaaa acgaaagcgt
      241 attggtgtta cttaccgaca tgaccttgtt tgcagatgct ttaagtattg tatcaaatag
      301 aatggatcaa attccatcta aagacagtat gcccggttcg ttgtattcag acttagctcg
      361 tatttacgaa aaagcagtgc aatttccatc gggaggttcc attaccatta tagcagttac
      421 caccctatct ggtggcgata ttacacatgc tattccagat aatacagggt atattaccga
      481 aggacaatta ttcttgcgtc gcgataccga aattggaaaa gtaattgttg acccattccg
      541 ctcattatca cgtttaaaac aattggttat tggcaaacaa acacgcgaag accatccgca
      601 agtgatgaat gcagccattc gtttgtacgc agatgcatca aatgcacgca ccaaaatgga
      661 aaacggattt gacctaacag actatgacga acgtacgcta gaatatgcca aagcctattc
      721 agaaaaacta ttggcaattg atgtaaatgt agataccgaa accatgcttt ctatctcttg
      781 ggatttattt agtacacatt tctcgaaagc agaagtaggt attaaagcag caattactga
      841 aaaatattgg aaaaattaag caccctagta gtttatggcg ataaaatttc aatacaataa
      901 aacctcttta caacacctcg acaaacaact taaggtaagg aaaagagcac taccaacgct
      961 gaaaaacaaa gaatcagcct tgcgtagcga agtaaaacgc gccaaagata aagcagcaac
     1021 gttagaggaa gagtttgaca aagaatggaa taaatacacc tatatggtaa agctatgggg
     1081 agaattcgac caatcgttaa tacgcgtaga ggatgtagta ttatcttcaa aaaaaattgc
     1141 aggtgtgctc acgcccttgt tagatgaagt gaaattttct attgctccat acaatccttt
     1201 taccgcccca atgtggtttg ctgatggcat cgaaatactt cgatcactag caactattgg
     1261 aattaaccgt gctgtatggc tacgcaaaat ggagttactg gaatttgcac gcaaaaaaac
     1321 tacgcaaaaa gtaaacttgt acgaaaaagt gcaaatacct ggttttgaag aa
//
LOCUS       DAOSMY010000361         1370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_96774_length_1370_cov_2.806084, whole genome shotgun sequence.
ACCESSION   DAOSMY010000361 DAOSMY010000000
VERSION     DAOSMY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96774_length_1370_cov_2.806084"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1253
                     /gene="nspC"
                     /locus_tag="PLD76_07060"
     CDS             123..1253
                     /gene="nspC"
                     /locus_tag="PLD76_07060"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOU27795.1"
                     /translation="MNLSQIPSPCFVLDEKLLRANLLLIQSVKEQAEVEIILAFKAFA
                     MWKVFPIIREYIPYSTASSLSEARLAYEEMGSKAHTYAPVYKPEEFDAIAACSSHITF
                     NSVAQYNQYAAVCKSKSISCGLRINPEYSSVETDLYNPCAVGSRLGVSVANLGEILPQ
                     GIEGLHFHTLCESSSFDLEATLEVVEQKFGRFFSQIKWLNMGGGHLMTRKGYDTEHLI
                     NLLKSFKSKYPHIQLILEPGSAFLWQTGVLVSTVLDIVENRGVSTAMLDVSFACHMPD
                     CLEMPYKPRIVGATDVIEGKPTYRMGGNSCLSGDFVGEWSFEKPLKVGDKIVFEDMIH
                     YTMVKTTMFNGVSHPSIGMWTLEDKFELFRVFTYLDYKQRMA"
BASE COUNT          383 a          215 c          303 g          469 t
ORIGIN      
        1 atacaagaaa acttagttaa aacttatcgg ttgtaacata tactttggtt ttacatggta
       61 tttttcggtt tcacttcgta aataaactaa atttctgtac ttttgtaaaa aaaaatatcc
      121 gtatgaacct atcacagatt ccttctcctt gttttgtgct tgacgaaaag ttgttgcgtg
      181 ctaatttact ccttattcag tctgttaaag agcaagcgga agttgaaatt attcttgcat
      241 ttaaagcttt tgcaatgtgg aaagtgtttc ctattattcg agaatatatt ccatattcaa
      301 cggcaagttc tttgagtgag gcacgtttgg cttacgaaga gatggggagc aaagctcata
      361 cgtatgcacc cgtatataag ccagaagagt ttgatgctat tgctgcgtgt agtagtcata
      421 tcacgtttaa ttcagtagca cagtacaatc agtatgctgc tgtttgtaag agcaagtcaa
      481 tttcgtgtgg cttacgcatc aatcctgagt attcttctgt cgaaacagat ttatacaatc
      541 cctgtgcagt tggttcccgc ttaggtgttt ccgttgctaa tttaggcgaa attttgcctc
      601 agggaattga aggcttgcat tttcacactt tatgcgaatc aagttcgttt gacttagaag
      661 ctactttaga ggttgtagaa caaaagtttg gtcgtttctt ttctcaaatt aaatggttga
      721 atatgggtgg aggacattta atgactcgca aaggttacga taccgagcat ttaatcaatc
      781 tattaaaatc attcaaaagc aagtatcctc atattcagtt aattttagag ccagggagtg
      841 ctttcttgtg gcaaacgggg gtgttggttt ctaccgtgtt ggatattgta gagaataggg
      901 gtgtttctac ggctatgcta gatgtgtcgt ttgcttgtca tatgcccgat tgtttagaaa
      961 tgccttataa accgcgtatt gttggagcta cagatgttat tgaaggtaaa ccaacgtatc
     1021 gaatgggggg taatagttgt ctgtcgggcg atttcgtagg cgagtggtcg tttgaaaaac
     1081 cactaaaagt tggggataaa attgtgtttg aagatatgat tcattataca atggtcaaaa
     1141 caaccatgtt taatggtgtg tcgcatccaa gtattggtat gtggacacta gaagataagt
     1201 ttgagttgtt tcgtgttttt acttaccttg actataagca gaggatggca taatatactt
     1261 ttttcttttg caaattttga aaaatcaaga ttagcatgta tctttgcacc atatttgcga
     1321 aaatagctca gttggtagag cataaccttg ccaaggttag ggtcgcgggt
//
LOCUS       DAOSMY010000362         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_97205_length_1366_cov_2.932876, whole genome shotgun sequence.
ACCESSION   DAOSMY010000362 DAOSMY010000000
VERSION     DAOSMY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97205_length_1366_cov_2.932876"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLD76_07065"
     CDS             <1..1223
                     /locus_tag="PLD76_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27796.1"
                     /translation="NWGFAYNRAKTFDKNIRCSTNSNLSLGDFLANYTEISGNAAGYT
                     ASVFDEDNDVYRDQRVPWASVLAWDGMMIANPQDESNNSINNQYESYRNNLISQSIGG
                     GVQNTYTIAERGSVNEYAFSGGANISNILQLGVSFVLLNLDYQMESIFEEKFENNSTY
                     KYTSLYQTDGSGFSIKVGAIARPTQWLRIGGAFHSPSWYYIKDNNEGSIRSKIVTEVK
                     PDYNGSTPVGYGSYYLNTPMKGLANLGFVIGNYGFVGVDYEYTDYSTMKMKNNDIINS
                     YMVDQNNKIATNFDATHTIRVGTELRPIENVSVRLGYAMVTPAIKADAYRTYATNTTR
                     TDFDFTNDKSTNYITAGLGYRFGKSYMDVAYVLQTKLQDYYTYNPTYVNGFGAPVEIT
                     SKHNQILLTYGVRF"
BASE COUNT          473 a          209 c          256 g          428 t
ORIGIN      
        1 ttaattgggg ttttgcttat aatagagcaa aaacttttga taaaaacatt cgttgttcaa
       61 ctaatagtaa tttatcatta ggcgacttct tagccaatta tactgaaatt tctggaaatg
      121 cagcaggcta tactgctagt gtttttgatg aagataatga tgtatataga gaccaacgtg
      181 tgccatgggc ttcggtatta gcatgggatg gcatgatgat tgcaaaccca caagatgaat
      241 cgaataattc tatcaataac caatacgaat catatagaaa taatttaata agtcaaagta
      301 ttggaggtgg agttcagaat acgtatacca tagctgagcg tggatcagta aatgagtatg
      361 catttagtgg aggtgcaaat ataagtaata tactgcaact gggagtaagt tttgtgttac
      421 tcaatctgga ttatcaaatg gaatcaattt ttgaagaaaa atttgaaaat aatagcacat
      481 acaaatatac aagtttatat caaactgatg gttctggctt tagtattaaa gtaggagcta
      541 ttgcccgacc aacgcagtgg cttcgtattg gtggtgcatt ccactcccct tcttggtatt
      601 atatcaaaga caataacgaa ggatcaatta gaagtaaaat tgtaaccgaa gtaaagcctg
      661 actacaacgg atcaactcct gtgggctatg gttcttacta cctaaataca ccgatgaaag
      721 gactagctaa ccttggtttt gtaattggaa attatggatt tgttggagta gattacgagt
      781 ataccgacta ttcaacaatg aaaatgaaaa ataatgatat tatcaattcc tatatggtgg
      841 atcagaataa taaaattgct accaattttg atgctacaca taccattcgt gttggtactg
      901 aacttagacc aattgaaaac gtgtctgtaa gacttggata cgccatggta actccagcaa
      961 taaaagctga tgcatacaga acatacgcaa ccaataccac tcgtacagat tttgatttta
     1021 caaatgataa aagtacaaat tacattacag caggtttggg gtatcgtttt ggaaaatcgt
     1081 atatggatgt ggcttatgta ttgcaaacaa aattacaaga ttattatacc tataacccta
     1141 cctatgtgaa tggatttggg gctcctgtag agattactag caagcataat caaattttac
     1201 taacttatgg agttcggttt taaataacaa acaatgagaa caaaaaaatg gagcactttt
     1261 accaagttgc tccatttttt taagctgtta tttagatatt acaatatagc taataatgtg
     1321 gcattcttcg taattttctc tacggcttct atttgatcaa atgtat
//
LOCUS       DAOSMY010000363         1355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_98352_length_1355_cov_2.046154, whole genome shotgun sequence.
ACCESSION   DAOSMY010000363 DAOSMY010000000
VERSION     DAOSMY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98352_length_1355_cov_2.046154"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLD76_07070"
     CDS             complement(<1..711)
                     /locus_tag="PLD76_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU27797.1"
                     /translation="MIYFAYIVLGFALLQLLVVGVNWIFRQSLTQEYTHNDKLVSVLI
                     PARNEEKNIASILGDLRKQTYQHIEIIVLDDDSTDNTSSIIEQQIQHDSRVKLLASKG
                     LPKGWLGKNYACHQLAKEAKGEYLLFLDADVRFQPRLIGKLLHFSINKQKQLVSIFPK
                     QEMKSAGEWKTVPLMNYILLTLLPLILVRFPYFSSLSAANGQCMFFSQNSYQSILPHQ
                     QLKNTAVEDIEIARLYKKR"
     gene            complement(708..1253)
                     /locus_tag="PLD76_07075"
     CDS             complement(708..1253)
                     /locus_tag="PLD76_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU27798.1"
                     /translation="MHINTLLPTDTMDKPILLIGNHISWWDGFIVQYINQKLWKKKFH
                     FMMLEEQLKKHQFLNLTGGFSINKKSKEIIASLTYAQELLHTKNNLVLMFPQGKITSM
                     HQQTMVFEKGIERILQHASDTIEVVFMVNIIDYFSDEKPTLYIYLTNYQTDKPNHKDI
                     EKAFQDFYNSCLGTQQKRATE"
BASE COUNT          423 a          221 c          220 g          491 t
ORIGIN      
        1 tcgttttttg tataaacgtg caatttcaat atcttcaact gcggtatttt taagttgttg
       61 gtgtggtaaa atagactgat aactattttg cgaaaaaaac atacattgcc cattagctgc
      121 cgataatgat gaaaaataag gaaaccgaac taaaatgagc ggtagtaaag taagcagtat
      181 gtaattcatt aaaggaacag ttttccattc tccagcactt ttcatctctt gtttcggaaa
      241 aatggatact aattgttttt gtttgttgat actaaaatgc agcaatttac caatgagcct
      301 tggctgaaag cgtacatctg catctaaaaa aagcaggtat tctccttttg cttcttttgc
      361 caattgatga caagcatagt tttttcctaa ccagcctttt ggtagacctt tcgatgctag
      421 cagttttact cgcgaatcgt gctgtatttg ttgctcaata attgaggacg tattgtcagt
      481 cgaatcatca tcaagtacta taatctctat gtgttgatac gtttgtttgc gtaaatcgcc
      541 taaaatagat gctatgtttt tttcctcgtt tcgtgcagga attaatacag ataccaattt
      601 gtcattatgg gtgtattctt gtgtcaaact ttgacgaaat atccagttta cacctaccac
      661 gagcagttgt aacaaagcaa aaccaagcac tatgtaggca aaataaatca ttccgtagca
      721 cgtttttgtt gggtacctaa acaactgttg taaaaatctt gaaaggcttt ctcaatgtct
      781 ttatgatttg gtttatctgt ttgataatta gttaaataga tatatagggt aggcttttca
      841 tcagaaaaat aatcaattat atttaccata aatactacct caatggtatc gcttgcatgc
      901 tgtaaaattc gttcaatgcc tttttcaaaa accatcgttt gttggtgcat gctggtaatt
      961 tttccttgcg gaaacataag taccaaattg ttttttgtat gtaataattc ttgtgcatac
     1021 gtaagtgatg caataatctc ttttgatttc ttattaatag aaaaacctcc tgttaagttc
     1081 aaaaattgat gttttttcaa ttgctcttct aacatcataa aatgaaactt ttttttccat
     1141 aatttctgat tgatatattg tacgataaat ccatcccacc aacttatatg gttgccaatg
     1201 agtaaaatag gtttatccat agtgtccgtt ggtaaaagcg tattaatgtg cacatcttta
     1261 aagtgccttt ttattttcca aaaagaatag aaaataaaaa atgggtagat aaaccaatga
     1321 tgattcactt atttcaaatc ctaaatcttt tattt
//
LOCUS       DAOSMY010000364         1343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_99639_length_1343_cov_2.829969, whole genome shotgun sequence.
ACCESSION   DAOSMY010000364 DAOSMY010000000
VERSION     DAOSMY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99639_length_1343_cov_2.829969"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLD76_07080"
     CDS             complement(<1..1158)
                     /locus_tag="PLD76_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOU27799.1"
                     /translation="MRLSDLQTGEKAIIVKVLGHGNFRKRIIEMGFIKGKKVEVLLNA
                     PLKDPIKYKVMDYEVSLRRNEAELIRVISEKEALASSTTSNYFGTIEASSLDAIAKKT
                     GHIINVALVGNPNSGKTSFFNIASGSHERVGNYSGITVDAKEGHFTLGDYKFRIFDLP
                     GTYSLAAYSPEERYVRKHIFEETPDVIINIVSASNLERNLYLTTQLIDMDVPMVIALN
                     MYDELEKHNHTLNYLQLGKMIGVPIVPTVAVKGYNNEKNGIKHVFEKVIEVYEGNEPT
                     VRHIHINHGGDLQLAIDKLNDLIKEDAHFKSHIAARFFAVKLLEKDKEAEQYIHSLAN
                     QKDILGYTEKAIPFLENELKDDTESAIINAKYGFIAGALAETFTEGKKDAFY"
BASE COUNT          350 a          240 c          282 g          471 t
ORIGIN      
        1 gtaaaaggca tcttttttcc cttcggtaaa agtttcggca agagctcccg ctatgaaccc
       61 atattttgca ttgataatag ctgattcagt gtcgtctttt agctcatttt ccagaaaagg
      121 aattgctttt tcagtatatc ccaaaatgtc tttttggttt gccagcgagt gaatatattg
      181 ttcggcttct ttgtcttttt cgagaagttt aacagcaaaa aaacgagcgg caatatgact
      241 tttaaaatga gcatcttctt taatcaggtc gttgagtttg tcaatagcca gttgtaaatc
      301 gccaccatgg ttgatatgta tatggcgtac ggtaggttca ttcccttcgt aaacctctat
      361 tactttttcg aatacgtgtt tgattccgtt tttttcgttg ttgtatcctt ttacggcaac
      421 tgtgggcaca atgggtacac caatcatttt acctagttga aggtaattga gcgtatggtt
      481 gtgtttttcc agttcatcgt acatattgag tgctattacc ataggcacgt ccatgtcgat
      541 gagttgtgtt gtaaggtaca aattgcgttc gaggttagag gctgatacaa tattgatgat
      601 tacatcgggt gtttcttcaa aaatatgttt ccgcacatag cgttcttcgg gcgagtaggc
      661 agctaaggag tatgtgccag gaaggtcgaa aatacgaaat ttataatcgc caagtgtaaa
      721 atgtccttct ttggcatcta ccgtaatgcc actgtagttg ccaacacgct cgtggctacc
      781 tgaggcaatg ttgaaaaagg aagttttgcc agagtttgga ttccctacta aagctacgtt
      841 gataatatga cctgtttttt tagcaatggc gtctaacgaa gaagcttcta ttgtaccaaa
      901 atagttggat gtagtagagc tcgctaaggc ttccttttcg ctgataacac gtattaattc
      961 agcttcgtta cgacgtagag atacctcgta atccattacc ttgtatttaa tggggtcttt
     1021 taaaggagca ttgagcagta cttctacttt tttgcccttg ataaagccca tttcgataat
     1081 gcgtttgcga aaattgccat gtcctagaac ctttactata atggcttttt ctcctgtttg
     1141 caaatcggat agacgcattg attgtagttt ttagcttgca aaggtaagta ttttattttt
     1201 gctttgcaat gactataagt agtgaaaaat agcctgttga ctcactataa ctagtgatga
     1261 ggctattgta cgtcttcttg cttaattagg ttgttgaata gggcatatac agtaagtcca
     1321 gatatggatt taatccctgt ttt
//
LOCUS       DAOSMY010000365         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_99839_length_1341_cov_3.424572, whole genome shotgun sequence.
ACCESSION   DAOSMY010000365 DAOSMY010000000
VERSION     DAOSMY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99839_length_1341_cov_3.424572"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..>1341
                     /locus_tag="PLD76_07085"
     CDS             266..>1341
                     /locus_tag="PLD76_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HOU27800.1"
                     /translation="MEQVLIVVEKFFEDASSFLWGIPMIVLLFGTHLYLTVRTGFVQR
                     KIGTAIKLSFTSDKESAGDISQFGSLAIALAATIGTGNIIGIATAIALGGPGAVFWCW
                     LTGVFGIATKYSESLLAVKYRVKTESGTMIGGAMVVLDKVLKMRWLAVLFCVFTAIAA
                     FGIGNMTQANSISVLVEQHFAIPPYVTGIILAVSVGIVMFGGVKWVATVCKALVPTMA
                     LVYIIGCLLLLGLNYQYVGSAISLIISSAFSSDAVGGGFTGAAVMMAMRFGIARGLFS
                     NESGLGSAPIVAAAAQTKNAVRQALISATGTFWDTVVICALTGIVLVSSVLAFPDIDF
                     HHGPMLTSMAFSKLHVWGSSILTFG"
BASE COUNT          348 a          195 c          298 g          500 t
ORIGIN      
        1 ttccatttat ctggtttttt aaaaactccc gtcattaatt gagaatacct accaacgtgt
       61 gtaatcagat tcatagattt tgcttaaaaa tagttcaacc cacaaaggta attatttttt
      121 tattcttatt ttgttaatat ttattatact tttgtcgcaa caagcaatac gccattagtg
      181 tattgtttgc agtatcgtct ttttattttt tattaactgg gtgtagggtt cttgtaagta
      241 gttgatagtt aaataaataa attgtatgga acaagtctta attgttgtag aaaaattctt
      301 tgaagatgca agttcttttt tgtggggtat ccctatgatt gtgttgttgt ttggtacaca
      361 cttgtatctt actgttcgta caggttttgt acaacgaaag ataggtactg ctattaaatt
      421 atcgtttact tctgataaag aatcagcagg agatatcagt cagtttggtt cgctggcaat
      481 agccctagca gctactattg gcacaggcaa tattattggc attgctacag ccattgcctt
      541 aggtggtcca ggtgctgttt tttggtgttg gctaacaggt gtttttggta ttgctaccaa
      601 atattcggag tcgctattag ctgtaaaata tcgggttaaa accgaatcgg gaacaatgat
      661 tggtggagct atggtagttt tagataaggt tttaaaaatg agatggttgg ctgtgttatt
      721 ttgtgttttt acagccatag ctgcttttgg tattggtaac atgacacaag caaattcaat
      781 ctcggtattg gtagaacagc attttgctat acctccgtat gttacaggta ttatattggc
      841 agtaagtgtt gggatagtga tgtttggtgg tgtaaaatgg gtagcaactg tgtgcaaagc
      901 attagtacct acgatggctt tggtatatat tattggatgt ttgttactcc ttggtttaaa
      961 ttatcagtat gtaggttcag caatttcatt aatcatatcc tcggcttttt cttcggatgc
     1021 cgttggaggc ggatttactg gagcagcagt aatgatggca atgcgttttg gtattgctcg
     1081 tggattattt tctaatgaat cgggattggg ttcggcacct atagttgcag cagcagctca
     1141 aacaaaaaat gctgttcgtc aagctcttat ctcagctact ggtacatttt gggatacagt
     1201 ggttatttgt gctcttacag ggattgtact agtaagtagt gtattggctt ttccagatat
     1261 tgattttcac catggaccaa tgcttacaag catggcattt agtaaattgc atgtatgggg
     1321 atcgtctatt ttaacgtttg g
//
LOCUS       DAOSMY010000366         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_99897_length_1341_cov_1.889580, whole genome shotgun sequence.
ACCESSION   DAOSMY010000366 DAOSMY010000000
VERSION     DAOSMY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99897_length_1341_cov_1.889580"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..828)
                     /gene="truA"
                     /locus_tag="PLD76_07090"
     CDS             complement(58..828)
                     /gene="truA"
                     /locus_tag="PLD76_07090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU27801.1"
                     /translation="MNEQPYFRYFVYLAYNGSSYHGWQIQPNGISVQEVLEKSFSTLL
                     KQPIAITGAGRTDTGVHANMMVAHFDTHTAIDDCTRLVGKLNAFLPQSIAIFAIKQVQ
                     PTAHARFDALSRTYQYHITTQKNPFIHQVSCKVPPTIDVNKMNTAAEKLLQYADFTSF
                     SKLHTDVKTNICTIHQARWTQVSDTEWVFTIQADRFLRNMVRAIVGTLLEVGRGKLSI
                     SEFENIILAKDRSKAGSSAPAQGLYLVDIKYPDSIFIH"
     gene            complement(834..>1341)
                     /locus_tag="PLD76_07095"
     CDS             complement(834..>1341)
                     /locus_tag="PLD76_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU27802.1"
                     /translation="TENTPKFASLIKKCKSSISCSHLYIMYITSSTEEDFILAMYKHG
                     VSEVVNLHTPEAILQARANKLAEHIIRCTNAHHKEELSLDPLLLSVSKGDIILQFTKK
                     EFDILHLLSNHPNKTFSRSEIYQKVWGNGIVVSDRTLDVHISKIRTKIGEQFIQTYKG
                     VGYRFMPR"
BASE COUNT          374 a          232 c          291 g          444 t
ORIGIN      
        1 gaaataagaa caaatattag ccacatgaag tatttatcaa ttattaatgg taaatgatta
       61 atgaataaaa atagagtcag gatattttat atcaacgaga taaagtcctt gtgcaggggc
      121 tgaactaccg gctttagaac ggtctttagc taaaataatg ttttcgaatt cgctgatact
      181 taattttcct cgccccactt ccaacaacgt tcccacaatg gcgcgtacca tattgcgcaa
      241 aaatcggtcg gcttgtatgg taaataccca ttcagtatcg cttacttgtg tccatcgggc
      301 ttggtgtatg gtgcaaatat tggtttttac gtcagtatgt aatttgctaa aactcgtaaa
      361 atcagcatat tgcagtagtt tttctgctgc agtgttcatt ttatttacat caatggttgg
      421 tggtactttg caacttactt ggtggataaa aggatttttt tgtgtagtaa tgtggtattg
      481 ataggtacgt gagagtgcat cgaaccgagc gtgagcagtg ggttgtactt gtttgatagc
      541 aaaaatggca atggattgtg gtaaaaatgc gtttaacttg cctaccagcc gtgtgcaatc
      601 gtctatagcc gtgtgcgtgt cgaaatgggc aaccatcatg ttggcgtgta cacccgtatc
      661 tgtgcgtcca gctccagtaa tggcaatggg ttgttttagt agggtagaaa aagatttttc
      721 gagcacttct tgcacactta ttccattggg ttgtatttgc caaccgtgat acgagctacc
      781 attgtaggct agatacacaa aatagcgaaa ataaggctgt tcgttcatag agctcaacgt
      841 ggcataaaac ggtatcctac tcccttgtat gtttgaataa actgttcccc aatttttgtg
      901 cgaatcttac taatgtgtac atctaaggtt ctgtcactta caacaatgcc attaccccat
      961 actttttggt aaatctcgga gcgagaaaag gttttgttgg gatgattgga taataagtgt
     1021 aagatatcaa attctttttt tgtaaattgc agtataatat cacctttgct tacggataat
     1081 agtaaagggt cgagcgataa ttcttctttg tggtgggcat tggtacaccg tataatatgt
     1141 tcggcaagct tgttcgctct tgcttgcaga atagcttctg gggtatgcag atttaccact
     1201 tcgcttacac cgtgtttgta cattgcaagg ataaagtctt cctctgtaga ggaggtaata
     1261 tacataatat acagatgact acaggatatc gaagatttgc atttttttat taaactagca
     1321 aatttgggcg tattttccgt a
//
LOCUS       DAOSMY010000367         1329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_101222_length_1329_cov_2.422292, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000367 DAOSMY010000000
VERSION     DAOSMY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101222_length_1329_cov_2.422292"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PLD76_07100"
     CDS             <1..1023
                     /locus_tag="PLD76_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU27803.1"
                     /translation="YKVSFLKKVLSLLPYIGKIWKRKIKKQRQKATLQAYEKQLTQAG
                     VKFIYYIRQTECALPDFPFVATNWDIGHCSTYAFPELIADGEFERRTSFYTKVLPKAL
                     LVFAESEEGKNELIKYTTIDERKLKIVPIFAGNCVTTVVSEQEQADILATYSLTKDTF
                     FFYPAQFWAHKNHYTLLKSFAELVHAHPSYKLVLTGSDKGNLNYIKQLAQDWNIADKV
                     LFLGFLPIEHINTFFRNATALIMASHFGPSNMPPLEAMELACPVIASDTIGHKEMLGK
                     AALYFDAMDSNALLVAMNKMIAETPRYKQAIVERQKTSVFNVEYALNQINIHLKEAVS
                     IRQNWN"
BASE COUNT          457 a          227 c          227 g          418 t
ORIGIN      
        1 tataaagttt cctttttaaa gaaagtactt tctttattac cctacatagg caaaatttgg
       61 aaacgtaaaa taaaaaaaca acgacaaaag gctactttac aggcatacga aaaacagcta
      121 acacaagctg gtgtaaagtt tatctattat atccgacaaa ctgaatgtgc tttgcctgat
      181 tttccgtttg tagctactaa ctgggatatt ggtcattgta gcacctatgc ttttcccgaa
      241 ttaattgctg atggtgagtt tgaaagaaga accagttttt ataccaaggt attacccaag
      301 gctcttttag tttttgccga atcagaagaa ggtaagaacg agttaattaa atataccact
      361 attgatgagc gtaaacttaa aattgtacct atctttgcag gaaattgtgt tacaacagta
      421 gttagtgaac aagagcaagc cgatatttta gcaacatatt cgcttacaaa agatactttt
      481 ttcttttatc ctgctcagtt ttgggcacac aaaaatcatt acaccttact gaaaagtttt
      541 gctgagttgg tgcacgcaca tccgtcgtac aaattagtgc taacgggttc ggataaagga
      601 aatttgaact acatcaaaca attagctcaa gattggaata ttgccgataa ggtcttgttt
      661 ttaggttttt tacccataga gcatatcaat acctttttta gaaatgctac tgcccttatt
      721 atggcatctc attttggacc cagtaacatg ccaccactag aagcaatgga attagcatgt
      781 cctgtgatag cctcagatac cataggtcat aaagaaatgc taggaaaagc tgcactttat
      841 tttgatgcta tggatagcaa tgcattgtta gttgctatga ataaaatgat tgctgaaacc
      901 ccaaggtata agcaagccat tgttgaaaga caaaaaacct ccgtctttaa cgtagaatat
      961 gcattaaacc aaatcaatat ccatctcaaa gaggctgttt ccatacgcca aaattggaac
     1021 taatgtaatt gtcttctctc ttcccctcgc tgaaagcgag gggataatta tcttagcgtt
     1081 tgtaatgcgt tcttataatt tatcttctcg ttgaaaacga ggaggatagg aatatacaaa
     1141 gtataatcct gaattttgca caacaaaata tcttaaatct gcacaataga aaagctcaat
     1201 tttgcacaat taaaaattac ttgtatcttt gtaggctatg ttttgttaca aaattaagta
     1261 taaataacct atattatgat acaaataaag tcgagtttaa ggtatagaat gtgataaata
     1321 taaaacaat
//
LOCUS       DAOSMY010000368         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_103131_length_1312_cov_3.163087, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000368 DAOSMY010000000
VERSION     DAOSMY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103131_length_1312_cov_3.163087"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLD76_07105"
     CDS             complement(<1..247)
                     /locus_tag="PLD76_07105"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOU27804.1"
                     /translation="MIGTLVNTAAVIVGGSLGMFIKHRLPEKYVRIYFQAVGLFTIVL
                     GISMSLKMAFPLMVVASLACGGILGEWLQLEEKTNRLG"
     gene            293..1198
                     /locus_tag="PLD76_07110"
     CDS             293..1198
                     /locus_tag="PLD76_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU27805.1"
                     /translation="MSEKYGYLLVNMLDMIKSYLTILVFIFVSMNVLAEQEHKMPPSN
                     TIGICLSGGGALGFSHIGVLQALEDNGIKPAYISGSSMGAIIGVLYAQGISPKEMMKI
                     IPKEKMNRFSRIWKLPSSILGFSSMITVHELLSKYVPHNRFDHLQIPLFVCVTNLYSG
                     EWEIKSTGDRLIDYVCASAAIPSVFEAQIIDSMHYVDGGVLNNLPVEPLLDKCDVIIG
                     VDVLEYPRKKELKKASDVVLYSILAANAETHRVRKPYCNYYISCNGASTYSIFDFHAF
                     KELYEIGYQRTVEYIQDHPEMLLHR"
BASE COUNT          381 a          237 c          275 g          419 t
ORIGIN      
        1 cgcccagtcg gtttgttttt tcttcgagct gaagccattc gccgagtatg ccgccacacg
       61 ccaaactggc tactaccatc aagggaaatg ccatttttag cgacatgcta atgcccaata
      121 ctatggtaaa caacccaacc gcttgaaaat agatacgcac gtatttttcg ggcaaacggt
      181 gtttgataaa catacccaaa ctgcctccca ctattaccgc agctgtattt actagtgttc
      241 caatcatctt attttaatta attcgacaca aaaataacaa aataattgct ttgtgtctga
      301 aaaatacgga tatttgttgg taaatatgct tgatatgata aaaagctacc ttacaatttt
      361 agtatttatt tttgtatcta tgaacgtatt agcagaacaa gaacataaaa tgcccccatc
      421 gaataccatt ggtatttgtt taagcggagg aggagcctta ggtttttcgc acattggtgt
      481 gttgcaagct ctcgaagaca atggtataaa acccgcctat atttcggggt cgagtatggg
      541 tgccataatt ggggtattgt atgctcaagg catttcgccc aaagagatga tgaaaattat
      601 tccgaaagag aaaatgaatc gcttttcgcg tatttggaaa ttaccaagtt ctattttggg
      661 tttttcaagt atgattacgg tacacgaact attgtcaaaa tatgttcccc ataatcgttt
      721 tgaccacttg caaataccct tgtttgtgtg tgtaactaac ctgtattcag gcgaatggga
      781 gattaaatca acgggagata gattgattga ttatgtgtgt gcatcggctg ctataccctc
      841 tgtgtttgag gctcagatta tcgactccat gcactatgta gatgggggtg tattaaataa
      901 tttgcccgtt gagccattat tggataagtg cgatgtgatt attggtgtag atgtgttgga
      961 atatcccaga aaaaaggaat taaaaaaagc ctctgatgtc gttttatatt ctattttagc
     1021 agccaatgcc gaaacccatc gggtgcgaaa accctactgc aattactata tatcgtgcaa
     1081 tggtgcttct acctattcga tttttgattt tcatgccttc aaggagttgt atgagattgg
     1141 ctaccagcgt actgtagagt atatacagga tcatccagag atgttgttgc atagatgaag
     1201 actggttgat agttgatagt tgatagttga tagttggagc tgtttaaagt tcattacccc
     1261 tctttggtgc aagtttagcg atagcgtaac ttgtacctat aataaaagca gt
//
LOCUS       DAOSMY010000369         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_105222_length_1295_cov_2.257258, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000369 DAOSMY010000000
VERSION     DAOSMY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105222_length_1295_cov_2.257258"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLD76_07115"
     CDS             complement(<1..416)
                     /locus_tag="PLD76_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27806.1"
                     /translation="MCQFVDAIQYSNGEFKRIAYHQKRAEAAVKEYYPLSNVFSIMDY
                     LRQLELPTTGIYKLRICYTDTIQSYECIPYKKREIKELHLVETHIKSRKYKLADRSDY
                     TIANKFDLNNSDILFVRDGLLTDTSYANIALLKNGI"
     gene            complement(400..>1295)
                     /locus_tag="PLD76_07120"
     CDS             complement(400..>1295)
                     /locus_tag="PLD76_07120"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOU27807.1"
                     /translation="LHHAIALLADEIDAENILYKIGGYTNESMQNDSLPENLVWQPQP
                     LSFADYSKKCTYVKEQIQLGNSFLVNLTQPTPIQTNVSLQDLYQLTDAKYKLWVKNHF
                     VVLSPEPFIRIQGLHISSFPMKGTIDASIPNAEKIILENPKEEAEHATIVDLIRNDLS
                     MIATDVHIKRYRYIEHIHSLQKHLLQVSSEICGTLPSNYHENLGTMIRKLLPAGSISG
                     APKTKTLDIIQSAEGYDRNFYTGICGYFDGENLDSAVMIRFIEQQDSQLVYKSGGGIT
                     ANSDIQTEYEELIQKIYVPIR"
BASE COUNT          361 a          213 c          245 g          476 t
ORIGIN      
        1 cagatgccat tttttaacaa tgcaatattt gcataactgg tatcggtcag taaaccatct
       61 cttacgaata gtatgtctga attatttaaa tcgaatttgt tcgcaatagt gtaatcactc
      121 ctgtcggcta atttgtattt acggctttta atatgtgttt ccactaaatg aagttcttta
      181 atttctcttt ttttatatgg tatacattcg taagattgaa ttgtatctgt atagcaaatg
      241 cgtagtttgt atatcccagt tgttggtaac tctaactgac gtagataatc catgatagaa
      301 aaaacattgg ataaaggata atactctttt acagcagctt cggcacgttt ttggtggtag
      361 gctattcgtt taaactcacc attactatat tgtatggcat caacgaattg gcacataaat
      421 cttttgtatt agttcttcgt attcagtttg tatatcacta ttagctgtta taccaccacc
      481 acttttgtat actaattgac tgtcttgttg ttcaataaat cgaatcataa cagcactatc
      541 caaattttct ccatcaaagt agccacaaat acctgtataa aaattgcggt cgtagccctc
      601 ggctgattgt ataatgtcaa gtgttttggt ttttggagca ccgctaattg aacctgcagg
      661 taatagtttt cgtatcattg tgcctaaatt ttcgtggtag ttactaggca atgttccaca
      721 tatttcggaa cttacttgca atagatgttt ttgcaatgaa tggatgtgtt caatataccg
      781 ataccgtttg atatgtacat cggtggctat catgcttaaa tcatttcgaa ttaaatcaac
      841 aatagtagcg tgttcggctt cttctttggg gttttctaaa attatttttt ctgcattagg
      901 aatagaagca tcaattgttc ctttcatagg gaatgaagaa atgtgtaagc cttgtatgcg
      961 aataaatggc tcgggagaca acacaacaaa gtggtttttt acccaaagtt tgtattttgc
     1021 atctgttagt tggtacaaat cttgtaagga tacattcgtc tgaatgggtg ttggttgtgt
     1081 taagtttacc aaaaacgaat tgcctaattg aatttgctct tttacatagg tgcatttctt
     1141 gctgtaatct gcaaatgaga gtggttgtgg ttgccaaact agattttcgg gtagcgaatc
     1201 attctgcata gattcgttgg tatacccacc aattttgtaa aggatgtttt ctgcgtcaat
     1261 ttcgtctgct aaaagtgcta tagcgtgatg caggt
//
LOCUS       DAOSMY010000370         1289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_105902_length_1289_cov_2.714749, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000370 DAOSMY010000000
VERSION     DAOSMY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105902_length_1289_cov_2.714749"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLD76_07125"
     CDS             complement(<1..261)
                     /locus_tag="PLD76_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU27808.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPSSVIKELVENAIDAGATSI
                     QIIIKDAGRTLIQVIDNGKGMSETDARMSFERHATSKITNAAD"
     gene            complement(293..565)
                     /locus_tag="PLD76_07130"
     CDS             complement(293..565)
                     /locus_tag="PLD76_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27809.1"
                     /translation="MPSFFHRPKPRQFHLTPRYYDEHKERIAEARKRVAQNSENKEEL
                     ADKPYYSAIKGKFGNVSNNLKSKNRQQIGMGIAIGILVLVLYFFFW"
     gene            complement(584..1288)
                     /locus_tag="PLD76_07135"
     CDS             complement(584..1288)
                     /locus_tag="PLD76_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOU27810.1"
                     /translation="MDWIKEENPDVLCFQETKAQPEQIDTSLFEALGYHCYFHSAEKK
                     GYSGVAILSKTKPDNVVIGMGNPKYDSEGRVIRADFGDISVMSVYVPSGTTGGIRQDF
                     KMEFLYDFTTFIHNLRLSYPKLVISGDYNIAHKPIDINHPERQVGVSGFLPEERDWLT
                     DFLESGFTDSFRAIDPSPEKYSWWSYRAGARTRNMGWRIDYHLISQALVAQLKHAAIL
                     SDVVHSDHCPVRIDLT"
BASE COUNT          373 a          258 c          241 g          417 t
ORIGIN      
        1 atcagcagca ttggtgattt tggatgtagc atgacgctca aaactcatgc gagcatctgt
       61 ttctgacatc ccttttccat tatcaataac ctgtattagg gttctgcctg catctttaat
      121 aatgatttga atagaagtag cacctgcatc aatagcattt tctactaact ctttgataac
      181 agacgagggg cgttgtataa cttcacctgc tgctatttgg ttagctaccg agtcgggtaa
      241 taaatggatt atatctgaca tagtgttgtg tacttattct acaaaagaac cattaccaaa
      301 agaaaaaata aagaaccaat accagaatgc caatagctat acccataccg atttgttgac
      361 ggtttttgct tttgaggtta ttacttacat tgccaaattt accttttata gccgaatagt
      421 aaggtttgtc tgctagctct tccttatttt cactgttttg tgctacacgt ttgcgtgctt
      481 cagcaatacg ctctttgtgt tcatcataat accgaggggt tagatgaaat tgacgtggtt
      541 ttggacgatg aaaaaaactt ggcataatca gtttcgttta attttacgtt aaatcaatgc
      601 gtactgggca atggtcagaa tgaacgacat cacttaaaat ggcagcgtgt tttagttgtg
      661 caactaaggc ttggctgata agatgatagt cgatacgcca acccatattt ctggttctgg
      721 ctcctgctcg ataactccac caactgtatt tttcaggtga agggtcaata gccctaaatg
      781 agtcggtaaa accgctttct aaaaagtcag tgagccagtc gcgttcttcg ggcaaaaatc
      841 ccgaaacacc tacttgcctt tcagggtgat tgatatcaat aggcttgtgt gcaatgttat
      901 aatcgccact aattactagt ttaggatacg agaggcgtaa attatgaata aaggtagtaa
      961 agtcgtataa aaattccatt ttaaaatctt gccgaatgcc cccagtagta cccgatggca
     1021 cgtatacact catcacactt atatcaccaa aatcagctct gataacgcga ccttcgctat
     1081 cgtatttggg attgcccatg ccaataacta cattgtcggg ttttgttttg ctcaaaatag
     1141 ctacaccact gtaccctttt ttttcagctg aatgaaaata acaatgatat cctaatgctt
     1201 caaatagact tgtatcaatc tgctcaggtt gagctttggt ttcttgaaag cataatacat
     1261 caggattttc ttcttttatc caatcaatt
//
LOCUS       DAOSMY010000371         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_107134_length_1279_cov_2.807190, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000371 DAOSMY010000000
VERSION     DAOSMY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107134_length_1279_cov_2.807190"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLD76_07140"
     CDS             <1..778
                     /locus_tag="PLD76_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU27811.1"
                     /translation="MAAVLAQGKTTIYKAACEPYLQQLCKMLNAMGAKISGIGSNLLT
                     IEGVESLGGCEHTILPDMIEIGSFIGMAAMTNSSITIKNVSYENLGIIPDSFRKLGIN
                     IEVSGDDIIVPKQSGFTIESYIDGSIMHLADAPWPGLTPDLLSVLLVVATKAKGSVLI
                     HQKMFESRLFFVDKLIDMGAQIILCDPHRATIIGLGENFRLRKAVMSSPDIRAGIALL
                     IAAMSAEGTSVIHNIEQIDRGYEDIELRINQLGGKIKREA"
     gene            complement(814..>1279)
                     /gene="tpx"
                     /locus_tag="PLD76_07145"
     CDS             complement(814..>1279)
                     /gene="tpx"
                     /locus_tag="PLD76_07145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOU27812.1"
                     /translation="TNGDLPKVGSVAPNFTLVNTGLADVSLADYKGKNVVLSINPSLD
                     TSVCAATARKFNTTLSQLANTVVLYITKDLPFAHNRFCTTEGIENVVTLSDYRNPEFA
                     TTYGVLVQDGALKGLMARAIVVINPQGVVTYTELVPEITTEPNYDNVIAALK"
BASE COUNT          403 a          242 c          265 g          369 t
ORIGIN      
        1 aatggctgcc gtattagctc aaggcaaaac gactatctac aaggcggcat gcgaaccgta
       61 tttgcaacaa ttatgtaaga tgctaaatgc catgggagca aaaataagtg gtattggctc
      121 taacttgctt acaattgagg gcgttgaatc attgggtggt tgcgaacata ccatattacc
      181 cgatatgatt gaaattggta gttttatcgg tatggcggct atgaccaact cttctatcac
      241 aattaaaaat gtatcatacg aaaacctggg tatcatccct gatagttttc gcaaattagg
      301 cataaacatc gaagtaagcg gtgatgatat tatcgtgcca aagcaatcgg gatttacaat
      361 cgaatcgtat atcgatggct ctatcatgca tttagccgat gctccttggc ccggattaac
      421 acccgacttg cttagtgtat tattggtagt agctaccaaa gccaaaggga gtgttttgat
      481 acatcaaaaa atgttcgaaa gtcgcctatt ttttgtagat aaactcattg acatgggtgc
      541 tcaaatcatc ttatgcgacc cgcatagagc tacaattatt ggattgggag aaaatttcag
      601 gttacgcaag gcagtaatga gttcgcctga tattcgtgct ggtatagcct tgctaatagc
      661 agcaatgagt gccgaaggaa caagcgtaat acacaatatt gaacaaatag acaggggata
      721 cgaggatata gaattacgca tcaaccaatt aggtggtaaa ataaaaagag aagcataaag
      781 cttctctttt tattttcaga caattgagtg ctgttatttt aaagcagcaa tgacattgtc
      841 gtaattaggt tcggtggtaa tttctggaac caactcggta tacgtaacca ctccttgtgg
      901 attaataacc acgatagcac gtgccattaa accttttaag gctccatctt gtactaacac
      961 gccatacgtt gtagcaaact caggattacg ataatcggat aaagtaacca cattctcaat
     1021 gccttctgtt gtacaaaaac gattgtgtgc aaaaggtaaa tctttggtga tgtacaacac
     1081 cactgtattc gctaattgcg acaaggtggt gttaaatttg cgtgcagtag ctgcacatac
     1141 agacgtatcc aaactgggat tgatactcaa aactacattc tttcctttgt aatctgctaa
     1201 cgaaacatct gctaagcctg tattcactaa ggtaaaatta ggtgctactg aacctacttt
     1261 aggtaaatct ccattggta
//
LOCUS       DAOSMY010000372         1255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_110208_length_1255_cov_2.660833, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000372 DAOSMY010000000
VERSION     DAOSMY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110208_length_1255_cov_2.660833"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLD76_07150"
     CDS             <1..575
                     /locus_tag="PLD76_07150"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOU27813.1"
                     /translation="AFYKFPVEIAFQLPSSQKTAILTSYFSNQSAMATSRYNTPVQLM
                     MVDTLGNYMQYQTSSASTTELKTITATNGKTYIFHNAITCGPVCDSHITIFDENWYYV
                     PIQQIAPALIKLQVSDFFDVTTATSEGIDIADLLTSIRPVFYELNFAIQHNDLLVTLH
                     LNALPETTRQQLQPYYHQTIRLSWNGERFE"
     gene            568..810
                     /locus_tag="PLD76_07155"
     CDS             568..810
                     /locus_tag="PLD76_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27814.1"
                     /translation="MNKEKNLSTQKKKQLKTYNPAKHHRPSIRLKGYNYVQSGLYSIP
                     ICCQNRACVFGHIENGIMIMNDAGKMVENEWLTLAH"
BASE COUNT          450 a          304 c          212 g          289 t
ORIGIN      
        1 aagcctttta caaatttcca gtagaaatag cgtttcaact acccagttca caaaaaacag
       61 ctatccttac cagctatttt tctaaccaat ctgctatggc tacaagtagg tacaacacac
      121 ctgtgcaact gatgatggta gacacccttg gcaactacat gcagtaccaa acttcatcag
      181 caagcactac cgaactcaaa accattaccg ccaccaatgg caaaacctac atttttcata
      241 atgccattac ctgcggaccg gtatgcgact cgcacattac catttttgac gagaattggt
      301 actatgtgcc cattcagcaa attgcaccag ccttgataaa attgcaagta agcgattttt
      361 ttgacgttac tacagccaca tcagagggta tagatattgc tgacttactt accagcatac
      421 gtcccgtatt ttacgaactc aattttgcta ttcagcacaa tgatttgctc gtaactctgc
      481 accttaacgc cttgccagaa accacacgcc agcaactaca gccctattac caccaaacta
      541 tacgcctatc atggaatggc gagcgatttg aataaagaga agaatctctc aacgcaaaag
      601 aaaaagcaat taaagaccta taaccctgcc aaacatcacc gccccagtat acgcctgaaa
      661 ggatataatt atgtacaatc aggattgtat tccataccca tttgttgcca aaacagggca
      721 tgcgtatttg ggcatattga aaatgggata atgataatga acgatgccgg gaaaatggtt
      781 gaaaacgaat ggttaacatt agcacattga ttgaaacgaa tcccataatg gcaaccccca
      841 accgtcaacc aaaatagaac gaatcacgta ggggcgaccc tcgtggtcga cccaaaataa
      901 aaacgattcc ccaaaccgcc gaccaaaata atatgaaagg gcaaccacaa gggattgccc
      961 caaccaaaaa cccatcacaa gggattgccc caaccgaaaa tccataacca ccatagaata
     1021 tatacgtggg ataaaaaaga ataattggca agcaatagat aaaagatttt ggcaacgaaa
     1081 ttaccacgaa cacatcattc gcaatgaaca atcgtatata catatatcaa attacatcat
     1141 acataaccct gcaaattggg ataaagatgt attgcaataa atctataaat ctgaataact
     1201 gatagtgtag cgaccgtatg caaatggtag catagcggac atcccgctca aacgg
//
LOCUS       DAOSMY010000373         1230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_113627_length_1230_cov_2.544681, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000373 DAOSMY010000000
VERSION     DAOSMY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113627_length_1230_cov_2.544681"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PLD76_07160"
     CDS             complement(<1..1041)
                     /locus_tag="PLD76_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27815.1"
                     /translation="MKKTAFLVASLIGCLFFAQAQDNVSTEKPLIPVVTLSKDVNVKF
                     GGFMRAEYYVDSKEMVGAFDDLFGFFPEKPVYDTNGKDINNVVRHNFSTQATRFNALF
                     TGPSVFNAKSSAFFEFDFTRGNSVNLRYRHAYGQLSWTKTDVLFGKSWNPLAAIHFPS
                     VLGLHTAIPFRPFGRGDQVRVTYRLTDKISILGAALIQTEHRSTLETSAATDVRANPI
                     PDMHLQLFHKTNNLMLGLLSEYKITRPATFTEGAGTSKPKYITKETVSSFSLGGIMDY
                     KKDLFNAKASVLYGQNLSELYIAGGYATTSIDATTGHRTYSPSNSVSSWVNFTYGKKW
                     IAGVFGGYHKNLG"
BASE COUNT          377 a          212 c          235 g          406 t
ORIGIN      
        1 ccctaaattt ttgtggtacc ctccaaaaac acctgctatc cattttttac catatgtaaa
       61 gtttacccac gatgacactg agttagaagg actgtaggta cggtgtcctg tagtagcatc
      121 aatagaagta gttgcatatc cgccagcgat gtataattcg cttaggtttt gaccatacaa
      181 aacacttgct ttggcattaa ataaatcttt tttgtaatcc ataattccac ctaaggaaaa
      241 agaagaaacg gtttctttgg taatgtattt tggttttgaa gttcctgcac cttcggtaaa
      301 agtagctgga cgtgtaattt tatattcaga cagtaaaccc agcatcaggt tgtttgtttt
      361 atgaaataat tgcaaatgca tatcaggtat tgggttagca cgtacatctg tagcagccga
      421 agtttctaag gtgctacggt gttcggtttg tattaaggca gcacccaaaa tgcttatctt
      481 gtctgtgagt ctgtaagtaa ctcgtacttg gtcgcctcta ccaaacggac gaaacggaat
      541 ggctgtgtgt aatcccaata ctgatggaaa atggatggct gctaaggggt tccacgattt
      601 accaaataga acatctgttt ttgtccatga taattgacca taagcatgac gatagcgtag
      661 gttaacgcta tttcctctgg taaaatcaaa ttcgaaaaat gccgatgatt ttgcattaaa
      721 caccgatggt cctgtaaaca aggcattaaa acgtgtagct tgtgtggaga aattgtgacg
      781 tactacattg tttatatctt ttccatttgt atcatatacg ggtttttcag gaaaaaagcc
      841 aaataaatca tcgaatgctc ctaccatttc tttggagtca acatagtatt cagcacgcat
      901 aaagccgcca aattttacgt tcacatcttt gcttaatgta acaacgggaa ttagcggttt
      961 ctctgtactt acattgtctt gtgcctgagc aaaaaatagg catccaatga gtgatgctac
     1021 taaaaaagct gttttcttca tagtgctttt tattagttat ctgttatctt gttatcgaat
     1081 tgtgtaaaaa actgattaac tgtaaatttt gtcgccgcaa aagtaagtat ttgttttagg
     1141 ttatgcaaat ttggtaataa aataaaaata gttaatagta tacatcggaa aaattatttt
     1201 tagttacata tttgcaacaa tgatgcaaat
//
LOCUS       DAOSMY010000374         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_116681_length_1208_cov_3.127493, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000374 DAOSMY010000000
VERSION     DAOSMY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116681_length_1208_cov_3.127493"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLD76_07165"
     CDS             <1..775
                     /locus_tag="PLD76_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU27816.1"
                     /translation="ELYANEATNEFMPDIYTTINSKKYKGNIEQYVNDTFKKSFITNE
                     VRFNKYLDKPSDKQFNNDPIILLSKSILKKRAEIARIAFADEKEIEHLTALLIEGYQR
                     KNKENTLLADANYTLRMSYGTITTYSPSDGVFFGYTSNFEGIIEKNNTRHAAYKIDKR
                     LVDIYQSKHNKNEKPFAVSFLTSIDYTIDKMGAPVFDAEGNLIGILSDGNNETISNIY
                     HYNERFQRTIALDIRYMLFILEHYAGAKRVIEKLNLVDE"
     gene            768..>1208
                     /locus_tag="PLD76_07170"
     CDS             768..>1208
                     /locus_tag="PLD76_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU27817.1"
                     /translation="MNNKIRVIADCHIPFLQGTIDNIATVTYLAASEISNRTVKDADA
                     LLIRTRTHCNESLLNSSKVKFIGTATIGYDHIDTQYCLQKGIVWKNAPGCNAASVAQY
                     IVAALLYMARKHSLSLTESTIGIIGVGHVGKQVAQMAETLGMRVL"
BASE COUNT          433 a          235 c          210 g          330 t
ORIGIN      
        1 cgaactctat gccaacgaag caacaaacga gtttatgccc gacatatata ctactattaa
       61 tagtaaaaaa tacaaaggaa atattgaaca atacgtaaac gatacattta aaaaatcatt
      121 tattaccaac gaagtacgct tcaacaaata ccttgacaaa ccctctgaca agcaatttaa
      181 caacgaccca atcattctat tgagcaaaag tatattaaaa aaacgtgccg aaatagcccg
      241 tattgctttt gctgacgaaa aagaaattga acatttaacc gcattattaa tagaaggcta
      301 ccaacgaaaa aacaaagaaa acaccttgtt agcggatgct aattacacct tacgtatgag
      361 ttatggcacc attactacgt atagcccctc agacggagta ttttttggct atacatctaa
      421 ttttgaagga attatcgaaa aaaataatac acgccacgcc gcctataaga tagacaaacg
      481 cttagtagat atttaccaaa gcaaacacaa taagaatgaa aaaccttttg cggtatcctt
      541 tcttacatct attgattata ctatagataa aatgggagct cccgtatttg atgcagaagg
      601 aaatttaatt ggcatactaa gtgatggaaa taacgaaacc atcagcaata tataccacta
      661 caacgaacgt tttcagcgaa ctattgccct tgatatacga tatatgttgt ttattcttga
      721 acattatgct ggagcaaaac gtgtgattga aaaattaaac ctcgttgatg aataacaaaa
      781 tacgtgtaat agctgactgt catatcccat ttttacaggg gactattgac aatattgcta
      841 ctgttactta cttagctgcg agtgaaataa gcaatagaac agtgaaagat gccgatgcac
      901 tacttattcg cacacgcact cactgcaacg aatcgctttt aaactctagt aaggtaaaat
      961 ttattggcac tgctaccatt ggctacgacc atatcgatac ccaatactgc cttcaaaaag
     1021 ggattgtatg gaaaaatgct ccagggtgca acgctgcttc ggtagctcaa tatatcgttg
     1081 ctgcattgct gtatatggca cgcaaacatt ctctatcgct tacagaaagt actattggca
     1141 ttattggtgt aggacatgtg ggcaagcaag tagcacaaat ggcagaaact cttggtatgc
     1201 gtgtgttg
//
LOCUS       DAOSMY010000375         1206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_117010_length_1206_cov_2.874891, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000375 DAOSMY010000000
VERSION     DAOSMY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117010_length_1206_cov_2.874891"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLD76_07175"
     CDS             <1..751
                     /locus_tag="PLD76_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27818.1"
                     /translation="INNPEYVYIYNDTPSEPNPTINIRVTQRWNPWDYSYFTPYYYPS
                     YHWSWNSNYGWGYTYNTYYPFYSTYPSSFYYGYYSPGYGYYGYGYYGYPSYYHHRPYY
                     YHHPYHHKPTYTTRKGYNNYDRTPNYVNDGRGSSSSYGYQNSGQRSTRSVGTNPTNTN
                     ASSTRNTSTYNNRERTFNSAPSSSTQNTRKSDVTTNTSTPRETSSGRSFNSSSSSSNS
                     NNSGTRSTQRSDNSSSSNGGSRSNGGRSFSR"
     gene            781..>1206
                     /locus_tag="PLD76_07180"
     CDS             781..>1206
                     /locus_tag="PLD76_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27819.1"
                     /translation="MKKIIITSLCALGIMSFANAQFQSEYDALKVAQTDLNGTARFMS
                     VAGAMGALGGDASTLFYNPAGIATYRSSELTFTGNWNWNNTSALGSMASRFLFNLNNV
                     SYISAYKTGSENGLVAFNWGFAYNRAKTFDKNIRYSTNSN"
BASE COUNT          411 a          236 c          203 g          356 t
ORIGIN      
        1 tatcaacaac ccagaatatg tgtacatata caacgacaca ccctctgaac caaatccaac
       61 tataaatatt cgtgttacac aacgctggaa tccatgggat tatagttatt ttacacctta
      121 ttattatcca tcctatcatt ggtcatggaa ctcaaactat ggatggggtt atacatacaa
      181 cacctactat ccattttata gtacatatcc ttcatcattt tattatggtt attattctcc
      241 aggttatggt tattatggtt atgggtacta tggttaccca tcttattatc atcatagacc
      301 ctactactat caccatccct atcatcataa acctacttac acaacaagaa agggctataa
      361 caattacgac cgcacaccca attatgtaaa tgatggcaga ggcagtagct cttcatatgg
      421 gtatcaaaat tctggacaac gttcaacacg ttctgttgga acaaacccaa caaatactaa
      481 cgcatctagt acacgtaata catcaactta caataataga gagcgtacat ttaatagtgc
      541 accatcttct tctacacaaa ataccagaaa aagtgacgta acaacgaata cctctacacc
      601 aagagaaaca tctagcggta gaagtttcaa ctcctcttca tcatcgtcta atagcaataa
      661 ttctggtaca agaagtacgc aaagaagtga taattcatcg tcttccaacg gaggaagcag
      721 aagcaatgga ggtcgctcgt ttagcagata gtaattatga aataaataaa aaataatacg
      781 atgaaaaaaa taataattac aagtttgtgt gcattaggaa ttatgagctt tgcaaatgct
      841 caatttcagt cggaatacga tgcactaaaa gtagctcaaa ctgacttaaa tgggacagca
      901 cgttttatgt ctgtggcagg agcgatggga gctttaggtg gagatgcctc taccctattt
      961 tacaatccag ctgggattgc tacgtaccgc agttctgagc ttacctttac tggaaactgg
     1021 aattggaaca atacctcagc tttaggcagt atggctagcc gttttttatt caatttgaat
     1081 aatgtatctt atatcagtgc atataaaaca ggatctgaga atggcttagt ggcttttaat
     1141 tggggttttg cttataatag agcaaaaact tttgataaaa acattcgtta ttcaactaat
     1201 agtaat
//
LOCUS       DAOSMY010000376         1206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_117034_length_1206_cov_2.463944, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000376 DAOSMY010000000
VERSION     DAOSMY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117034_length_1206_cov_2.463944"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1206)
                     /gene="proS"
                     /locus_tag="PLD76_07185"
     CDS             complement(<1..>1206)
                     /gene="proS"
                     /locus_tag="PLD76_07185"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOU27820.1"
                     /translation="KSFLSKEAEHVEGFAKECAVVTHYRLKNAEDGSGVVVDPAAKLE
                     EELIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVFRWEMRTRMFLRTAEFLWQEG
                     HTAHATKEEAEEEALKMLHVYGDFAENFMAVPVVKGIKTANERFAGALETYTIEAMMQ
                     DGKALQSGTSHFLGQNFAKAFDVQFITKENKQEYVWATSWGVSTRLMGALIMTHSDDN
                     GLVLPPKLSPIQVVIVPIYKGEEQLQAINEKVDGIVKKLKALGISVKYDNADNKRPGF
                     KFAEYELKGVPVRLVMGGRDLESNTIEVTRRDTFEKQTRSCDDIDIFVKQLLDDMQQT
                     ILQKALDLRTSLTCTVETYDEFKEKIEEGGFILAHWDGTSETEEKIKEETKATIRCIP
                     LEGDKTPGV"
BASE COUNT          331 a          273 c          191 g          411 t
ORIGIN      
        1 cacacaccag gagttttatc tccctctaac ggaatacaac gaatggttgc tttggtttct
       61 tccttaattt tttcttcagt ttctgaagta ccatcccaat gggcaagtat aaaaccacct
      121 tcttcaattt tttctttaaa ctcgtcgtac gtttccacag tgcaagttag agatgttctc
      181 aaatcaagtg ctttttgcaa aatagtttgt tgcatatcat ccaacaattg tttcacaaaa
      241 atatctatat catcacaact acgtgtttgt ttttcaaacg tatctctacg agtaacttct
      301 atggtattgc tttctaaatc gcgaccaccc attaccaatc gtacaggcac tcctttgagt
      361 tcgtattcgg caaacttaaa tcctggtcgt ttgttgtcag cattgtcgta ttttacactt
      421 ataccgagtg ctttcaattt ttttacgata ccatctactt tttcgttaat ggcttgtagc
      481 tgttcttctc ccttataaat aggtacaatt accacctgaa taggggataa ttttggaggc
      541 aataccaatc cattatcatc gctatgtgtc attatcaatg ctcccatcaa acgggtagaa
      601 acaccccacg aagtagccca cacatattct tgtttgtttt ctttggtaat aaactgcaca
      661 tcaaaagctt tcgcaaaatt ttgtcctaaa aagtgcgaag tacccgattg taaggctttt
      721 ccatcttgca tcatagcttc aatggtatag gtttcgaggg ctccagcaaa acgttcgttg
      781 gctgttttta tgccttttac aacaggaact gccataaaat tctctgcaaa atcgccatat
      841 acgtgtagca tttttaatgc ttcttcttca gcctcttctt tagtagcatg agcagtatgc
      901 ccttcttgcc acaaaaattc tgctgtacgc aaaaacatcc gtgtacgcat ttcccaacga
      961 aatacgtttg cccattgatt acataaaatg ggtaaatcgc ggtacgattg tatccaattg
     1021 cggtaagtat tccaaataat agtttcggag gttggacgaa taattaattc ctcttctaac
     1081 ttggcagctg ggtcaaccac tacacccgat ccatcttcag cattttttaa tctataatga
     1141 gttaccacgg cacattcttt cgcaaaacct tctacgtgct cagcttcttt acttaaaaaa
     1201 gatttg
//
LOCUS       DAOSMY010000377         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_132551_length_1111_cov_2.985795, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000377 DAOSMY010000000
VERSION     DAOSMY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132551_length_1111_cov_2.985795"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLD76_07190"
     CDS             complement(<1..214)
                     /locus_tag="PLD76_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27821.1"
                     /translation="MKNINWNGFSPFIHLPHTGFIKFITKIYLIIYIYNRLPKFLSNI
                     NYIATNTNNEHSRAKKTAHSNLSCCLI"
     gene            545..946
                     /locus_tag="PLD76_07195"
     CDS             545..946
                     /locus_tag="PLD76_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU27822.1"
                     /translation="MWCLSLHAYAVNWDKLLVSYETGDTTKITKKYTIAISGVYAFVG
                     DKKREVIVLKNDGNTWKPMQILKPTTKKRVEFGKKITIWNDVVIIEAPKESKNGTIYV
                     YRIVNNRWRRVQKIKNALRRAMLLYTNDWHC"
     gene            complement(1054..1111)
                     /locus_tag="PLD76_07200"
     tRNA            complement(1054..1111)
                     /locus_tag="PLD76_07200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1088..1090),aa:Arg,seq:acg)
BASE COUNT          383 a          154 c          197 g          377 t
ORIGIN      
        1 ttattaaaca gcacgataaa ttactatgtg ctgttttttt tgctctggag tgttcgttat
       61 tcgtgttggt tgcaatatag ttaatattcg ataaaaattt gggtaatctg ttgtaaatat
      121 aaataattag atagattttt gttataaatt ttataaaacc agtatgtggt agatgaatga
      181 atgggctaaa gccattccaa ttgatatttt tcacatcccc tttatcattt cctcaataaa
      241 tgtctatatt cgcaaatatt cgcaagtttt tccaagaaaa agaaattctt gctaaaaatg
      301 aaacggaata cattcccaac ttaatgattg aatgagcaag aaaaacgatg tcttccttta
      361 aacgagttaa gccaaaaata atgtatcttt gcgatcgatt aaaatacatg ctagagattg
      421 atacgattat gctagataga ttatatgtat actttgttat gaaagctgag atgaatgcta
      481 tgaatgttaa attgctatat acaaaataat gaaaaatctt ttgtacatag tttttattac
      541 actaatgtgg tgtttgtctt tacatgcgta tgctgttaat tgggataaac tattagtgtc
      601 gtatgaaact ggagatacca caaagataac gaaaaaatat acgattgcta ttagtggggt
      661 atatgctttt gtaggagata aaaaacgtga agtaatcgtg ctaaaaaatg atggaaatac
      721 ctggaaaccg atgcaaattc ttaaacctac taccaagaaa cgcgttgagt ttggcaaaaa
      781 aattacgatc tggaatgatg tcgttatcat cgaggctcct aaggaaagca aaaatggaac
      841 gatatatgtg tatcgtattg tgaataatag atggagaaga gttcagaaaa taaaaaacgc
      901 cctacgccgt gcaatgttgc tctatacgaa tgattggcat tgttaatatc tccgatattt
      961 acgcttttat cctctatttc tgttgtaaat ctattgaaat aaaaaaacac ctacaagtac
     1021 ttttgtaggt gttttttgtg cttttatttg tgaccgcgat aggattcaaa cctataacct
     1081 tctgatccgt agtcagatac tctattcagt t
//
LOCUS       DAOSMY010000378         1110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_132795_length_1110_cov_2.169668, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000378 DAOSMY010000000
VERSION     DAOSMY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132795_length_1110_cov_2.169668"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PLD76_07205"
     CDS             complement(<1..1057)
                     /locus_tag="PLD76_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HOU27823.1"
                     /translation="MGISTIRSYRGAQLFEALGISSELLSKYLHGTITKLEGIGLEDI
                     HRDIVACHQQAFASELELLSNSGQYAYRRNGEYHAWNPETIAKLQIATRTGDYAKFKE
                     YTRHVDEKEQTVFIRDLIDYKRNPIDISLVEPIENITSGYRMDISHGFCGSDCTNRTD
                     IMFDYYNNGVKAGNEQAYFILEHWEFGDGERENYNQWGMNCHLNNTNSYMQTAMGWLK
                     DGDGLDASNQDGFISYCESHDEERTMFKAKSYGNGATLRTLAGYCNRVPLNTAFNVLL
                     NGSHMMWMFQELGYDYSIFSNAQGGTGDRVDPKPIPESLNWYTNTTRMNAYQKVGQLI
                     QLRTRILPHVFAGDPTNS"
BASE COUNT          325 a          225 c          185 g          375 t
ORIGIN      
        1 cggagttagt agggtcgcca gcaaatacgt gtggtaaaat acgcgtacgt agttgaatta
       61 attgtcccac tttttggtat gcattcatac gggtagtgtt cgtataccaa tttaaacttt
      121 cgggtattgg tttagggtca acacgatctc ctgtccctcc ttgagcattg ctaaatatac
      181 tataatcata gcctaattct tggaacatcc acatcatatg cgaaccattt aatagtacat
      241 taaatgctgt atttaagggt acacgattgc aatagcccgc taaagtacgt aaagtggcac
      301 cattaccata cgattttgct ttaaacatag tgcgttcttc gtcatggctc tcgcaataag
      361 aaataaatcc gtcttggttt gatgcatcta gtccatctcc atctttcaac caacccatag
      421 ctgtttgcat ataactattc gtattgttta gatggcaatt catgccccat tggttgtagt
      481 tttcgcgttc tccatctcca aattcccaat gttcaagaat aaaataagct tgttcgtttc
      541 ctgctttcac accattgttg taataatcaa acataatatc tgtacggttg gtacaatcag
      601 agccacaaaa accatgcgaa atatccatac gataaccact agtaatattt tcgataggtt
      661 ctaccaaaga aatatctata ggattacgtt tataatcaat caagtcacga ataaacactg
      721 tttgttcttt ttcgtccaca tgacgcgtat attccttaaa tttagcataa tcacctgtgc
      781 gggttgctat ttgtaacttg gcaatggttt caggattcca agcgtggtat tctccattgc
      841 gtctgtatgc gtattgacca ctattagaaa gtaattctaa ttcagatgca aaagcttgtt
      901 gatgacacgc tacgatatct cgatgaatat cttctaatcc aatgccttct aattttgtga
      961 tagttccatg tagatatttg ctaagcaact cagacgaaat acctaaagct tcaaacaatt
     1021 gtgctccacg gtaactacga attgtagaaa tacccatttt agaaattact tttttcagcc
     1081 ctttgtttaa agactttatg taattttttt
//
LOCUS       DAOSMY010000379         1027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2385
            NODE_150006_length_1027_cov_3.739712, whole genome shotgun
            sequence.
ACCESSION   DAOSMY010000379 DAOSMY010000000
VERSION     DAOSMY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864452
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:42:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,442
            CDSs (total)                      :: 1,423
            Genes (coding)                    :: 1,403
            CDSs (with protein)               :: 1,403
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 7 of 20
            Pseudo Genes (incomplete)         :: 12 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150006_length_1027_cov_3.739712"
                     /isolate="AMR_MDS_2385"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="PLD76_07210"
     CDS             complement(<1..>1027)
                     /locus_tag="PLD76_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU27824.1"
                     /translation="FAHLDFVRVNAYQALKMQGNSMYFRQTDQNVRYDVLQYELGNAT
                     SDIRIWDLSDPLNMVEIPTTFSTNTLFFVPSVRSDLLVREFLAIRTSATFPKPEVIGK
                     IANQNLHAIQQVDYVIISPNEFREQAMRLATFHQQKNGYSVVIVEPQQIYNEFSSGTP
                     DATAYRWFLKMLYDRAGDNINNQPKALLLFGLSSHDNRGLVYSRMPLLSYQSFNSFDS
                     HISYTTDDYYAFLDDSEGVNIIQDIMDVGVGRLPVATKEEAKIVVDKIINYANNTKKG
                     SWKNLLAFLGDDGDSNDHMEQSDNLVQKWQEKWNEYQPIKVFLDSYQMTQTASGSTYP
                     GARDRMLN"
BASE COUNT          332 a          204 c          165 g          326 t
ORIGIN      
        1 aattgagcat cctgtctctg gctccaggat atgtacttcc acttgcagtt tgcgtcattt
       61 gatatgagtc taaaaacact ttaattggtt gatattcatt ccatttctct tgccattttt
      121 gcactaaatt gtcagactgt tccatgtggt cattggaatc accatcatcc cctaaaaaag
      181 caagcaaatt tttccaagat cctttttttg tattgttggc ataattgata attttatcta
      241 ccactatttt agcttcttct ttagttgcga caggcagacg acccacccca acgtccataa
      301 tatcttgtat gatatttacc ccctctgaat catccaaaaa agcataataa tcgtcagttg
      361 tataagaaat atgagagtca aaagaattga atgattgata actcaataaa ggcatacgag
      421 aatacacaag cccacgatta tcgtgagatg ataatccgaa aagcaacaaa gccttaggtt
      481 gattgttgat attatctcca gctctatcat acaacatttt tagaaaccat cgatacgctg
      541 tagcatcagg tgttcctgaa gaaaattcat tataaatctg ctgaggttct actatcacta
      601 cagaataacc atttttttgt tgatgaaatg tagccaaacg cattgcctgt tctctaaatt
      661 catttggaga aataattaca taatccactt gttgaatcgc atgtaaattt tgattggcta
      721 ttttaccaat tacttctggt tttggaaatg tagccgacgt gcgtatagcc aaaaattctc
      781 gcaccaacag atcagagcga acagaaggga caaaaaacag cgtattagtc gagaatgtag
      841 ttggtatttc taccatattc agaggatcgg ataaatccca gatacgtata tccgaagtag
      901 catttcctaa ctcatactgc aatacatcat accgtacatt ttgatctgtt tgcctaaaat
      961 acatactatt cccttgcatt ttaagagctt gataagcatt tacacgaaca aaatcaagat
     1021 gagcaaa
//
